A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions.
The property of blood capillary ENDOTHELIUM that allows for the selective exchange of substances between the blood and surrounding tissues and through membranous barriers such as the BLOOD-AIR BARRIER; BLOOD-AQUEOUS BARRIER; BLOOD-BRAIN BARRIER; BLOOD-NERVE BARRIER; BLOOD-RETINAL BARRIER; and BLOOD-TESTIS BARRIER. Small lipid-soluble molecules such as carbon dioxide and oxygen move freely by diffusion. Water and water-soluble molecules cannot pass through the endothelial walls and are dependent on microscopic pores. These pores show narrow areas (TIGHT JUNCTIONS) which may limit large molecule movement.
Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures.
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.
Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.
Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.
The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
A species of the Beta genus. Cultivars are used as a source of beets (root) or chard (leaves).
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
A group of FLAVONOIDS derived from FLAVONOLS, which lack the ketone oxygen at the 4-position. They are glycosylated versions of cyanidin, pelargonidin or delphinidin. The conjugated bonds result in blue, red, and purple colors in flowers of plants.
Any technique by which an unknown color is evaluated in terms of standard colors. The technique may be visual, photoelectric, or indirect by means of spectrophotometry. It is used in chemistry and physics. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Compounds derived from TYROSINE via betalamic acid, including BETAXANTHINS and BETACYANINS. They are found in the Caryophyllales order of PLANTS and some BASIDIOMYCETES.
Natural or synthetic dyes used as coloring agents in processed foods.
Conjugates of betalamic acid with cyclo-dopa, both of which derive from TYROSINE. They appear similar to INDOLES but are biosynthesized by a different path and contain N+. Members are red or violet COLORING AGENTS found in the Caryophyllales order of PLANTS and some BASIDIOMYCETES.
Conjugates of betalamic acid with AMINO ACIDS. Some of them are yellow COLORING AGENTS in the Caryophyllales order of PLANTS. This should not be confused with xanthin which is a term used for CAROTENES nor with XANTHINES.
Any spaces or cavities within a cell. They may function in digestion, storage, secretion, or excretion.
A plant genus of the family ANACARDIACEAE. This is the source of the familiar cashew nuts, which are heat treated to remove the irritant toxin. Cashew nut shell liquid (frequently abbreviated as CNSL) is a major source of alkenyl phenolic compounds, especially ANACARDIC ACIDS, cardol, and cardanol.
Solutions that have a greater osmotic pressure than a reference solution such as blood, plasma, or interstitial fluid.
Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)
A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
The movement of materials across cell membranes and epithelial layers against an electrochemical gradient, requiring the expenditure of metabolic energy.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
Time period from 1601 through 1700 of the common era.
Time period from 1801 through 1900 of the common era.
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
Time period from 1701 through 1800 of the common era.

Functional consequences of mutations in the human alpha1A calcium channel subunit linked to familial hemiplegic migraine. (1/7242)

Mutations in alpha1A, the pore-forming subunit of P/Q-type calcium channels, are linked to several human diseases, including familial hemiplegic migraine (FHM). We introduced the four missense mutations linked to FHM into human alpha1A-2 subunits and investigated their functional consequences after expression in human embryonic kidney 293 cells. By combining single-channel and whole-cell patch-clamp recordings, we show that all four mutations affect both the biophysical properties and the density of functional channels. Mutation R192Q in the S4 segment of domain I increased the density of functional P/Q-type channels and their open probability. Mutation T666M in the pore loop of domain II decreased both the density of functional channels and their unitary conductance (from 20 to 11 pS). Mutations V714A and I1815L in the S6 segments of domains II and IV shifted the voltage range of activation toward more negative voltages, increased both the open probability and the rate of recovery from inactivation, and decreased the density of functional channels. Mutation V714A decreased the single-channel conductance to 16 pS. Strikingly, the reduction in single-channel conductance induced by mutations T666M and V714A was not observed in some patches or periods of activity, suggesting that the abnormal channel may switch on and off, perhaps depending on some unknown factor. Our data show that the FHM mutations can lead to both gain- and loss-of-function of human P/Q-type calcium channels.  (+info)

Maintenance of motility in mouse sperm permeabilized with streptolysin O. (2/7242)

One approach to studying the mechanisms governing sperm motility is to permeabilize sperm and examine the regulation of motility by manipulating the intracellular milieu of the cell. The most common method of sperm permeabilization, detergent treatment, has the disadvantage that the membranes and many proteins are extracted from the cell. To avoid this problem, we have developed a method that uses streptolysin O to create stable pores within the plasma membrane while leaving internal membranes intact. Sperm were permeabilized, preincubated, and then treated with 0.6 U/ml of streptolysin O. Permeabilization was assessed by fluorescent dye technologies and endogenous protein phosphorylation using exogenously added [gamma-32P]ATP. Streptolysin O-induced permeabilization rendered the sperm immotile, and the effect was Ca2+-dependent. When the cells were treated simultaneously with a medium containing ATP, streptolysin O-treated sperm maintained flagellar movement. These results demonstrate that the streptolysin O permeabilization model system is a useful experimental method for studying the mechanisms that regulate sperm motility since it allows the flagellar apparatus to be exposed to various exogenously added molecules.  (+info)

Gating connexin 43 channels reconstituted in lipid vesicles by mitogen-activated protein kinase phosphorylation. (3/7242)

The regulation of gap junctional permeability by phosphorylation was examined in a model system in which connexin 43 (Cx43) gap junction hemichannels were reconstituted in lipid vesicles. Cx43 was immunoaffinity-purified from rat brain, and Cx43 channels were reconstituted into unilamellar phospholipid liposomes. The activities of the reconstituted channels were measured by monitoring liposome permeability. Liposomes containing the Cx43 protein were fractionated on the basis of permeability to sucrose using sedimentation in an iso-osmolar density gradient. The gradient allowed separation of the sucrose-permeable and -impermeable liposomes. Liposomes that were permeable to sucrose were also permeable to the communicating dye molecule lucifer yellow. Permeability, and therefore activity of the reconstituted Cx43 channels, were directly dependent on the state of Cx43 phosphorylation. The permeability of liposomes containing Cx43 channels was increased by treatment of liposomes with calf intestinal phosphatase. Moreover, liposomes formed with Cx43 that had been dephosphorylated by calf intestinal phosphatase treatment showed increased permeability to sucrose. The role of phosphorylation in the gating mechanism of Cx43 channels was supported further by the observation that phosphorylation of Cx43 by mitogen-activated protein kinase reversibly reduced the permeability of liposomes containing dephosphorylated Cx43. Our results show a direct correlation between gap junctional permeability and the phosphorylation state of Cx43.  (+info)

Modulation of distal colonic epithelial barrier function by dietary fibre in normal rats. (4/7242)

BACKGROUND: Dietary fibre influences the turnover and differentiation of the colonic epithelium, but its effects on barrier function are unknown. AIMS: To determine whether altering the type and amount of fibre in the diet affects paracellular permeability of intestinal epithelium, and to identify the mechanisms of action. METHODS: Rats were fed isoenergetic low fibre diets with or without supplements of wheat bran (10%) or methylcellulose (10%), for four weeks. Paracellular permeability was determined by measurement of conductance and 51Cr-EDTA flux across tissue mounted in Ussing chambers. Faecal short chain fatty acid (SCFA) concentrations were assessed by gas chromatography, epithelial kinetics stathmokinetically, and mucosal brush border hydrolase activities spectrophotometrically. RESULTS: Body weight was similar across the dietary groups. Conductance and 51Cr-EDTA flux were approximately 25% higher in animals fed no fibre, compared with those fed wheat bran or methylcellulose in the distal colon, but not in the caecum or jejunum. Histologically, there was no evidence of epithelial injury or erosion associated with any diet. The fibres exerted different spectra of effects on luminal SCFA concentrations and pH, and on mucosal indexes, but both bulked the faeces, were trophic to the epithelium, and stimulated expression of a marker of epithelial differentiation. CONCLUSIONS: Both a fermentable and a non-fermentable fibre reduce paracellular permeability specifically in the distal colon, possibly by promoting epithelial cell differentiation. The mechanisms by which the two fibres exert their effects are likely to be different.  (+info)

Effects of inhibitors and substitutes for chloride in lumen on p-aminohippurate transport by isolated perfused rabbit renal proximal tubules. (5/7242)

The transport step for p-aminohippurate (PAH) from cell to lumen across the luminal membrane of rabbit proximal tubules has not been adequately defined. To examine this process more closely, we determined the effects of possible transport inhibitors and substitutes for chloride on PAH secretion in isolated perfused S2 segments of rabbit proximal tubules. The addition of 4-acetamido-4'-isothiocyano-2,2' disulfonic stilbene (10(-4) M) to the perfusate irreversibly inhibited PAH secretion, whereas the addition of probenecid (10(-4) M) to the perfusate reversibly inhibited PAH secretion. PAH secretion was unaffected by thiocyanate replacement of chloride in the luminal perfusate, reversibly inhibited by 15 to 20% by methyl sulfate replacement, and irreversibly inhibited by isethionate replacement. Because the luminal membrane is at least as permeable to thiocyanate as to chloride, less permeable to methyl sulfate, and much less permeable to isethionate, these data suggest that the PAH transport step from cells to lumen does not require chloride in the lumen but does require a highly permeant anion. During inhibition of PAH transport from cells to lumen, PAH uptake across the basolateral membrane was also reduced, suggesting some type of feedback inhibition. The data are compatible with PAH transport across the luminal membrane by an anion exchanger, a potential-driven uniporter, both carriers, or a carrier that can function in both modes.  (+info)

Increased lipophilicity and subsequent cell partitioning decrease passive transcellular diffusion of novel, highly lipophilic antioxidants. (6/7242)

Oxidative stress is considered a cause or propagator of acute and chronic disorders of the central nervous system. Novel 2, 4-diamino-pyrrolo[2,3-d]pyrimidines are potent inhibitors of iron-dependent lipid peroxidation, are cytoprotective in cell culture models of oxidative injury, and are neuroprotective in brain injury and ischemia models. The selection of lead candidates from this series required that they reach target cells deep within brain tissue in efficacious amounts after oral dosing. A homologous series of 26 highly lipophilic pyrrolopyrimidines was examined using cultured cell monolayers to understand the structure-permeability relationship and to use this information to predict brain penetration and residence time. Pyrrolopyrimidines were shown to be a more permeable structural class of membrane-interactive antioxidants where transepithelial permeability was inversely related to lipophilicity or to cell partitioning. Pyrrole substitutions influence cell partitioning where bulky hydrophobic groups increased partitioning and decreased permeability and smaller hydrophobic groups and more hydrophilic groups, especially those capable of weak hydrogen bonding, decreased partitioning, and increased permeability. Transmonolayer diffusion for these membrane-interactive antioxidants was limited mostly by desorption from the receiver-side membrane into the buffer. Thus, in this case, these in vitro cell monolayer models do not adequately mimic the in vivo situation by underestimating in vivo bioavailability of highly lipophilic compounds unless acceptors, such as serum proteins, are added to the receiving buffer.  (+info)

Elevated expression of the CD4 receptor and cell cycle arrest are induced in Jurkat cells by treatment with the novel cyclic dinucleotide 3',5'-cyclic diguanylic acid. (7/7242)

The effect of the novel, naturally occurring nucleotide cyclic diguanylic acid (c-di-GMP) on the lymphoblastoid CD4+ Jurkat cell line was studied. When exposed to 50 microM c-di-GMP, Jurkat cells exhibited a markedly elevated expression of the CD4 receptor of up to 6.3-fold over controls. C-di-GMP also causes blockage of the cell cycle at the S-phase, characterized by increased cellular thymidine uptake, reduction in G2/M-phase cells, increase in S-phase cells and decreased cell division. Additionally c-di-GMP naturally enters these cells and binds irreversibly to the P21ras protein. The effects described appear to be unique for c-di-GMP.  (+info)

Stimulation of strontium accumulation in linoleate-enriched Saccharomyces cerevisiae is a result of reduced Sr2+ efflux. (8/7242)

The influence of modified plasma membrane fatty acid composition on cellular strontium accumulation in Saccharomyces cerevisiae was investigated. Growth of S. cerevisiae in the presence of 1 mM linoleate (18:2) (which results in 18:2 incorporation to approximately 70% of total cellular and plasma membrane fatty acids, with no effect on growth rate) yielded cells that accumulated Sr2+ intracellularly at approximately twice the rate of S. cerevisiae grown without a fatty acid supplement. This effect was evident over a wide range of external Sr2+ concentrations (25 microM to 5 mM) and increased with the extent of cellular 18:2 incorporation. Stimulation of Sr2+ accumulation was not evident following enrichment of S. cerevisiae with either palmitoleate (16:1), linolenate (18:3) (n-3 and n-6 isomers), or eicosadienoate (20:2) (n-6 and n-9 isomers). Competition experiments revealed that Ca2+- and Mg2+-induced inhibition of Sr2+ accumulation did not differ between unsupplemented and 18:2-supplemented cells. Treatment with trifluoperazine (TFP) (which can act as a calmodulin antagonist and Ca2+-ATPase inhibitor), at a low concentration that precluded nonspecific K+ efflux, increased intracellular Sr2+ accumulation by approximately 3.6- and 1.4-fold in unsupplemented and 18:2-supplemented cells, respectively. Thus, TFP abolished the enhanced Sr2+ accumulation ability of 18:2-supplemented cells. Moreover, the rate of Sr2+ release from Sr2+-loaded fatty acid-unsupplemented cells was found to be at least twice as great as that from Sr2+-loaded 18:2-enriched cells. The influence of enrichment with other fatty acids on Sr2+ efflux was variable. The results reveal an enhanced Sr2+ accumulation ability of S. cerevisiae following 18:2-enrichment, which is attributed to diminished Sr2+ efflux activity in these cells.  (+info)

Introduction. Investigating the effect of temperature on cell membrane permeability To investigate the effect of temperature on cell membrane permeability, I am going to use beetroot due to its pigment properties. The pigment is a strong colour, which means that I can easily measure, using a colorimeter, how much pigment is released at certain temperatures. During this practical, I will need to apply controls and a variable. The variable being temperature, and the controls being: * Disc thickness * The amount of water used when heating * The measure of solution put into the cuvette * The time temperature is maintained If these factors were not controlled, results would be likely to produce false reading, due to too many variables. Apparatus Cuvette Colorimeter Beetroot disc Water bath Beaker Tripod Bunsen burner Boiling tube Cork borer Forceps Safety glasses Thermometer Stop clock Scalpel Pipette Method 1. Put on safety goggles as a safety precaution 2. Switch on colorimeter to allow it to warm ...
Cell membrane prevents the entrance of extra molecules (e.g., transcription and translation inhibitors) into the cell. For studying the physiological effects of transcription and translation inhibitors on Hymenophyllum caudiculatum fronds, we incubate fronds with 0.1% DMSO to test if this increases cell membrane permeability relative to incubation with ultrapure water. The study showed that DMSO could significantly improve the cell membrane permeability of filmy fronds.
What cell membrane permeability means, how it relates to metabolic rate (oxidation rate) and how to ensure optimal cell membrane permeability for nutrient absorption and toxin elimination. The cell wall permeability indicators in my hair mineral analysis are also discussed. The cell membrane is what surrounds each cell in our bodies. It is selectively permeable to ions and molecules.
ABSTRACT: Increased ammonium (NH4+) concentrations are reported to be an important cause of decline in submersed macrophytes in eutrophic lakes. In this study, a subacute experiment was conducted to examine the effects of various NH4+ concentrations on growth and membrane permeability of 2 submersed macrophytes, Myriophyllum spicatum and Ceratophyllum demersum. Apical shoots of the plants were incubated in modified Hoagland solution with 0.25, 0.5, 1, 2, 4, 8, 16, 32, 64 and 128 mg l-1 NH4+-N for 4 d. The plants were then collected for examination of cell membrane permeability and the solutions were used to determine the concentrations of NH4+, sodium (Na+), potassium (K+), magnesium (Mg2+) and calcium (Ca2+). The results indicate that high NH4+ concentrations had significant adverse effects (e.g. reduced growth and leaf chlorosis) on the macrophytes and increased membrane permeability, leading to net leakage of NH4+ and the cations. Compared to M. spicatum, C. demersum had higher membrane ...
Hopanoids modify plasma membrane properties, like fluidity and formation of liquid ordered domains, influencing membrane permeability, rigidity and other characteristics in bacteria, similarly as sterols (like cholesterol) do in eukaryotes.[5] This relationship between biochemical structure and cellular function can be seen in the similarity of the basic structures of diploptene, a hopanoid compound found in some bacterial cell membranes, and cholesterol, a sterol compound found in eukaryotic membranes.[5] In many bacteria hopanoids may play roles in the adjustment of cell membrane permeability in adaptation to extreme environmental conditions. They are formed in the aerial hyphae (spore bearing structures) of the prokaryotic soil bacteria Streptomyces, where they are thought to minimise water loss across the membrane to the air.[6] In the actinomycete Frankia, the hopanoids in diazovesicle membranes likely restrict the entry of oxygen by making the lipid bilayer more tight and compact.[7] ...
TY - JOUR. T1 - Femtosecond optoinjection of intact tobacco BY-2 cells using a reconfigurable photoporation platform. AU - Mitchell, Claire A.. AU - Kalies, Stefan. AU - Cizmár, Tomás. AU - Heisterkamp, Alexander. AU - Torrance, Lesley. AU - Roberts, Alison G.. AU - Gunn-Moore, Frank J.. AU - Dholakia, Kishan. PY - 2013/11/14. Y1 - 2013/11/14. N2 - A tightly-focused ultrashort pulsed laser beam incident upon a cell membrane has previously been shown to transiently increase cell membrane permeability while maintaining the viability of the cell, a technique known as photoporation. This permeability can be used to aid the passage of membrane-impermeable biologically-relevant substances such as dyes, proteins and nucleic acids into the cell. Ultrashort-pulsed lasers have proven to be indispensable for photoporating mammalian cells but they have rarely been applied to plant cells due to their larger sizes and rigid and thick cell walls, which significantly hinders the intracellular delivery of ...
Abstract. This study investigated the suppressive effect and the inactivation mechanism of slightly acidic electrolyzed water (SAEW) on . The experiment compared different inhibition effects with SAEW and two antibiotics, gentamicin and norfloxacin. The conditions of pH of 6.5, oxidation-reduction potential (ORP) of 890 mV, available chlorine concentration (ACC) of 28 mg L-1 of SAEW, and volume ratios of 1:10, 1:15, and 1:20 were used. Using exposure times of 5 and 10 min to analyze the inactivation mechanism of SAEW from the perspectives of cell membrane permeability, cell nuclear morphology, cell fluorescence staining, and other aspects, the anti-infection effects of SAEW were tested using tilapia () culture experiments. SAEW had a stronger bacteriostasis effect than the antibiotics used. SAEW can inhibit the ability to reproduce, causing abnormal cell morphology and cell elongation. The cell volume was increased with a change in the membrane permeability. The cell nuclear morphology was ...
Miltefosine is an orally- and topically-active alkyl-phosphocholine compound with potential antineoplastic activity. Miltefosine targets cellular membranes, modulating cell membrane permeability, membrane lipid composition, phospholipid metabolism, and mitogenic signal transduction, resulting in cell differentiation and inhibition of cell growth. This agent also inhibits the anti-apoptotic mitogen-activated protein kinase (MAPK) pathway and modulates the balance between the MAPK and pro-apoptotic stress-activated protein kinase (SAPK/JNK) pathways, thereby inducing apoptosis. As an immunomodulator, miltefosine stimulates T-cells, macrophages and the expression of interleukin 3 (IL-3), granulocyte-macrophage colony stimulating factor (GM-CSF), and interferon gamma (INF-gamma). Check for active clinical trials or closed clinical trials using this agent. (NCI Thesaurus).
Physical therapists at SOL Physical Therapy offer cold laser therapy. We are committed to help people recover faster in Oakland, CA.
Dr. Revici was a pioneer in chemistry and lipid-based cancer therapies, whose toxicity unlike conventional methods was negligent. Revicis unique interpretation of the periodic table of the elements and its applicability to human physiology allowed him to develop therapeutic, lipid and mineral-based, non-toxic formulations. These therapeutic lipids, when administered in the correct manner have powerful effects on the metabolism of the body.. Recognizing that cancer was either functioning as anaerobic (imbalanced anabolic) or dysaerobic (imbalanced catabolic), his focus was on shifting the individuals chemistry so that the cancer was not able to survive.. Dr. Revicis study of chemistry and lipids led to his discovery that elements and lipids act upon the cell membrane permeability in a particular way. An example of Revicis discoveries is that the trace mineral selenium showed to have a potent anti-anabolic effect at the cellular level, especially when delivered in a bi-valent, ...
It seems that in recent years, salt has gotten a very bad reputation - yet, the human body cannot live without salt. In fact, our bodies rely on it to help fight fungus, bacteria, and viruses. Its important in the regulation of blood pressure, the proper functioning of the nervous system and brain cells, metabolism, digestion, adrenal function, and cell membrane permeability. Its necessary to produce hydrochloric acid in stomach for digestion, reduces aldosterone (hormone released by adrenal glands to help regulate blood pressure) secretion in our endocrine (hormones that regulate bodys growth, metabolism, and sexual function) system, and it helps our body eliminate toxic substances by keeping them in solution. Theres so much more! However, not everyone realizes that there are different types of salt (refined and unrefined), and these differences will directly affect your health.. ...
Cellular effects of Laser Therapy occur when photons of therapeutic laser light interact with cells. Higher adenosine tri-phosphate (ATP) levels are produced, cell membrane permeability is increased, and blood and lymphatic vessels are vasodilated. These events then lead to a cascade of beneficial effects increasing cellular energy and health.. During each treatment, laser energy increases circulation drawing water, oxygen, and nutrients to the damaged area. This creates an optimal healing environment that reduces inflammation, muscle spasms, stiffness, and pain. As the injured area returns to normal, function is restored and pain is relieved.. ...
Results: 49. Adhesive Remover (Model 6197) Adhesive Remover, model 6197, is designed to dissolve skin cement and adhesives. It allows removal of appliances without irritating the skin. SIZE: Eight ounce can.. Adhesive Strips (Models 6590 & 150525) Adhesive Strips are catheter supplies designed for use with bulk pack male external catheters. Available 10 per box. Model 6590: Crixiline adhesive strip - 6 x 7/8 inch. Model 150525: Two-sided adhesive strip - 6 x 3/4 inch.. Amerigel Wound Hydrogel Dressing The Amerigel Wound Hydrogel Dressing is designed for use by individuals who have ulcers, venous stasis ulcers, diabetic skin ulcers, first and second degree burns, post-surgical incisions with dehiscence, cuts, and abrasions. The dressing decreases cell membrane permeability and balances the mix of proteins in the wound site. Proliferates proteins to the wound site and promotes the development of new granulation tissue growth. The dressing also promotes wound contracture.. Atrac-Tain Atrac-Tain is ...
Antifungals (Systemic) Action: Antifungals act by increasing cell membrane permeability in susceptible organisms by binding sterols and decreasing potassium, sodium, and nutrients in the cell. Uses: Antifungals are used for infection of histoplasmosis, blastomycosis, coccidiomycosis, cryptococcosis, aspergillosis, phycomycosis, candidiasis, sporotrichosis causing severe meningitis, septicemia, and skin infection. Side effects / Adverse reactions: The most serious …. ...
Developed in 2011. Atlantic Sea Scallop Dredge Design Challenge (Grades 9 & 10). The Cell Cycle and Mitosis (Grades 8 & 9). Conducting Action Research on the Impact of Environmental Issues on Community Health (Grades 9 & 10). Genetic Mutations (Grades (9 & 10). Kidney Dissection (Grades 9 & 10). Lifesaver Protein Modeling (Grades 9 - 12). Developed in 2010. Genetic Variation (Grades 9 & 10). Investigating Factors That Affect Rate of Enzyme Action (Grades 9 & 10). Micropipette and the Metric System (Grades 9 & 10). Interactive Meiosis (Grades 9 & 10). Modeling Protein Structure. Thinking Like a Scientist (Grades 9 & 10). Understanding Kidney Disease (Grades 3-5). Whose Brain is THAT?!. Developed in 2009. Bacterial Transformation. Blood Analysis. Blood, I Presume?. Concept Mapping. DNA Extraction From Living Things. Investigating Factors That Affect Cell Membrane Permeability. Lets Grow Some Microbes!. Sheep Brain Dissection. Developed in 2008. Amino acids and protein. Amoeba this!!!! Amoeba ...
TY - CONF. T1 - Supported liquid membrane permeation for selective lactic acid recovery. AU - Kienberger, Marlene. AU - Mungma, Nuttakul. AU - Siebenhofer, Matthäus. PY - 2017/11/8. Y1 - 2017/11/8. N2 - 1. IntroductionLactic acid is handled as a future bulk chemical due the use for poly lactic acid production. State of the art production for lactic acid is fermentation followed by precipitation and hydrolysis. Much attention has been drawn to the optimization of the fermentation as well as the downstream processing, however the best solutions has not been found yet [1]. Besides the state of the art production lactic acid is present in aqueous effluent from the pulping industry [2]. Following the biorefinery approach, valuables from the black liquor in pulping process may lead to a biorefinery including the carboxylic acids formic acid and acetic acid as well as lactic acid as valuables. This approach is applied in textile fiber production since long, but is new for the cellulose production. The ...
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Looking for cell permeability? Find out information about cell permeability. The permitting or activating of the passage of substances into, out of, or through cells, or from one cell to another. These materials traverse either the... Explanation of cell permeability
Selective Permeability - What is it? Selective permeability means that the cell membrane has some control over what can cross it, so that only certain molecules either enter or leave the cell.
Knowing of gas shale permeability reduction is vital for gas production from shale gas reservoirs stimulated by multistage hydraulic fracturing. However, the role of shale matrix permeability reduction is not well addressed for evaluating the contribution on gas well production. In this study, the experiments of slickwater treatment at different injection pressures, injection volumes, and slickwater compositions were conducted by using an experimental apparatus based on the steady-state method. The results show that slickwater flow in the core is seriously affected by microfracture. The cumulative gas volume through the core with microfracture increases nearly linearly with time. Then, we analyzed the effect of injection pressure gradients, injection volume, and slickwater compositions on the permeability reduction and flowback efficiency. The permeability reduction and flowback efficiency both increase slightly at beginning and then nearly linearly in the middle and gradually at last with the increase
Flow phenomena in porous media are relevant in many technological applications including wastewater filtration, chemical separations, and oil recovery. Nonwoven fibrous membranes are widely used as filtration media, and tailoring the microstructure of the pore space is a key to improving filtration efficiency and preventing membrane fouling. However, predicting effective properties such as permeability and tortuosity for complex porous microstructures remains a challenging task as most available models do not incorporate the influence of random fiber arrangement and orientation on the structure-property relations. This study presents a computational analysis of the permeability and tortuosity of realistic nonwoven fibrous media with regular and random fiber arrangements over a wide range of porosities. We conduct pore-scale lattice Boltzmann simulations to predict the fluid flow through nonwoven fibrous media and use the results to test the accuracy of semiempirical scaling relations. Combining ...
Flow phenomena in porous media are relevant in many technological applications including wastewater filtration, chemical separations, and oil recovery. Nonwoven fibrous membranes are widely used as filtration media, and tailoring the microstructure of the pore space is a key to improving filtration efficiency and preventing membrane fouling. However, predicting effective properties such as permeability and tortuosity for complex porous microstructures remains a challenging task as most available models do not incorporate the influence of random fiber arrangement and orientation on the structure-property relations. This study presents a computational analysis of the permeability and tortuosity of realistic nonwoven fibrous media with regular and random fiber arrangements over a wide range of porosities. We conduct pore-scale lattice Boltzmann simulations to predict the fluid flow through nonwoven fibrous media and use the results to test the accuracy of semiempirical scaling relations. Combining ...
90 degrees (Shirtcliffe et al., 2010). When introduced to moisture, a hydrophilic treated surface will cause the moisture to spread out as a thin layer on the surface. All Rights Reserved. On an average day here at HZO you hear people throw around words like, nanotechnology, and vapor deposition as casually as if they were talking about their neighbors cat. This problem has been solved! 3 answers. Some molecules have both hydrophilic as well as hydrophobic sections in their structure. Bri S. Hello, I am confused with the properties of polylactic acid (PLA). Hydrophilic vs. Hydrophobic. Show transcribed image text. Who is the longest reigning WWE Champion of all time? What is hydrophilic. The backbone of DNA is Hydrophilic Molecules: Hydrophilic molecules attract water molecules. A hydrophilic molecule or portion of a molecule is one whose interactions with water and other polar substances are more thermodynamically favorable than their interactions with oil or other hydrophobic solvents. ...
The introduction of peptides and other cell membrane-impermeable molecules into mammalian cells has been an invaluable biochemical approach in the study of intracellular processes. Numerous methods have been developed to introduce membrane-impermeable molecules into cells. These methods include treating cells with solvents such as toluene or dimethyl sulfoxide, hypotonic buffers, freeze/thaw techniques, electroporation, and various detergents.1 2 3 In addition, microinjection of peptides and proteins into cells has also been used.4 Two major concerns with these methodologies are that the permeabilization or microinjection itself does not compromise cellular intregrity and that a relatively high percentage of cells are permeabilized.. One method of cell permeabilization (referred to as skinning) involves the permeabilization of mammalian cell membranes by incubation of cells with detergents such as saponin. This technique has been used with smooth muscle cells5 and cells or tissue preparations ...
At a basic level, neurons generate electrical signals through brief, controlled changes Imagine taking two electrodes and placing one on the outside and the other on the inside of the plasma membrane of a living cell. Electric charge also plays an important role in membrane permeability, says Physiology Web. Diffusion does not require any energy as the molecules move from areas of high concentration to areas of low concentration, also known as moving down the concentration gradient. it grows in size and eventually ruptures.. Describe how membrane permeability, concentration gradient, and molecular properties affect biological Polar molecules and charged molecules do not diffuse easily through the lipid core of the plasma Several factors affect the rate of diffusion of a solute including the mass of the solute, the. In passive transport, substances move from Factors that affect diffusion. I believe the beetroots membrane denatured and a red pigment leaked from it.. ...
Principal Investigator:UCHIDA Shinichi,丸茂 文昭, Project Period (FY):1996 - 1998, Research Category:Grant-in-Aid for Scientific Research (A), Section:展開研究, Research Field:Kidney internal medicine
Buy Govazol Online! Govazol is an antifungal medication used in the treatment of several fungal infections. Govazol widely distributes into body tissues, nails and fluids including saliva, sputum, vaginal secretions. Without ergosterol, 14 alpha-methyl sterols increase which subsequently causes the fungal cell membrane to exhibit increased cellular permeability.
Physiology Test Question - At the peak of the action potential, plasma membrane permeability to Na+ (pNa) is higher than the permeability to K+ (pK).
Abnormalities of red cell membrane cation permeability are seen in several hereditary disorders. These dominantly inherited conditions are collectively called the hereditary stomatocytoses and allied disorders.
ACS Publications Biological membranes are perfect examples for a molecular filter that uses membrane channels to control the permeability of small water-soluble molecules. We started from the known mutant channel FhuA Δ1-160 in which the cork domain closing the channel had been removed to allow filtering of larger hydrophilic molecules. Here, we further expanded the pore diameter by copying the amino acid sequence of two β-strands in a stepwise manner increasing the total number of β-strands from 22 to 34. The pore size of the respective expanded channel protein was characterized by single-channel conductance analysis. Furthermore, polymer exclusion measurements were performed by analyzing single-channel conductance in the presence of differently sized polyethylene glycols of known polymer random coil radii. The conclusion from channel conductance of small channel penetrating polymers versus larger excluded ones suggested an increase in pore radii from 1.6 nm for FhuA Δ1-160 up to a maximum ...
Evaluation of cell permeability by fluorescence images of UHP treated cells under live/dead staining after pressure treatment at 3 hours. (a-e) NIH/3T3, (f
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View Notes - INFLAMMATION_notes from KINS 2532 at Georgia Southern University. INFLAMMATION Nonspecific Body Defenses First line of defense: surface membrane barriers Intact skin epidermis Intact
This kit gives students the opportunity to construct a model of a kidney and observe filtration of simulated kidney blood. Using a semipermeable membrane, students filter simulated kidney blood and test the solutions with simulated salt test strips. The simulated kidney blood also contains microscop...
This selection is possible because the structure of the plasma membrane is composed of a phospholipid bilayer which blocks all polar and charged particles while allowing nonpolar molecules and that act as transporters or channels for molecules that fit the proteins specific conformation.. ...
Permeabilize Eukaryotic Membranes - posted in Tissue and Cell Culture: Hi All, we try to treat eukaryotic cells with some antibiotics. Now it seems like some of the antibiotics (aminoglycosides) arent properly in the cells, so we are looking for some stuff to permeabilize the cells. Any good ideas? Links? Thx lots for your help
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Learn about Peripheral Proteins , Integral Proteins , Transmembrane Proteins , Fluid Mosaic Model , Selective Permeability , Channel Proteins , Transport Proteins , Recognition Proteins , Adhesion Proteins , Receptor Proteins .... http://cueflash.com/decks/Cliff\s_AP_Biology_Vocabulary:_Cells. Tags: ...
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Given that Chairman/CEO Frazier gave a very thoughtful, and yet candid presentation last week, it must be more than a little disappointing that Goldman kept its non-favored stance on the Whitehouse Station company: . . . .Goldman, Sachs & Co. reiterates Neutral rating on Merck. . . So it goes.
Vanden Abeele F, Bidaux G, Gordienko D, Beck B, Panchin YV, Baranova AV, Ivanov DV, Skryma R, Prevarskaya N. Functional implications of calcium permeability of the channel formed by pannexin 1 ...
Immunofluorescence Protocol without Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experimental procedure.
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Pulsed electric fields (PEFs) can be used to transiently increase cell membrane permeability in procedures ranging from gene therapy to tumor eradication. Although very efficient, PEF-based therapies generally require the use of invasive electrodes, which cause pain and tissue damage. An emerging noninvasive, contactless alternative to PEFs are High Intensity Pulsed Electromagnetic Fields (HI-PEMF), whereby the electric field inside the tissue is induced remotely by external pulsed magnetic field. However, one of the current major drawbacks of HI-PEMFs is their inferior efficiency compared to PEFs. In this study we present the proof-of-concept that by adding highly conductive 5 and 20 nm gold nanoparticles (Au NPs), we can significantly potentiate the permeabilizing effect of HI-PEMFs, making it possible to permeabilize up to 80% of the cells with minimal or no effect on cell survival, compared to negligible percentage of permeabilized cells using HI-PEMF alone. Experiments, conducted on Chinese ...
High-energy-density biofuels are typically derived from the fatty acid pathway, thus establishing free fatty acids (FFAs) as important fuel precursors. FFA production using photosynthetic microorganisms like cyanobacteria allows for direct conversion of carbon dioxide into fuel precursors. Recent studies investigating cyanobacterial FFA production have demonstrated the potential of this process, yet FFA production was also shown to have negative physiological effects on the cyanobacterial host, ultimately limiting high yields of FFAs. Cyanobacterial FFA production was shown to generate reactive oxygen species (ROS) and lead to increased cell membrane permeability. To identify genetic targets that may mitigate these toxic effects, RNA-seq analysis was used to investigate the host response of Synechococcus elongatus PCC 7942. Stress response, nitrogen metabolism, photosynthesis, and protein folding genes were up-regulated during FFA production while genes involved in carbon and hydrogen metabolisms were
Cell-penetrating peptides (CPPs) are prominent delivery vehicles to confer cellular entry of (bio-) macromolecules. Internalization efficiency and uptake mechanism depend, next to the type of CPP and cargo, also on cell type. Direct penetration of the plasma membrane is the preferred route of entry as this circumvents endolysosomal sequestration. However, the molecular parameters underlying this import mechanism are still poorly defined. Here, we make use of the frequently used HeLa and HEK cell lines to address the role of lipid composition and membrane potential. In HeLa cells, at low concentrations, the CPP nona-arginine (R9) enters cells by endocytosis. Direct membrane penetration occurs only at high peptide concentrations through a mechanism involving activation of sphingomyelinase which converts sphingomyelin into ceramide. In HEK cells, by comparison, R9 enters the cytoplasm through direct membrane permeation already at low concentrations. This direct permeation is strongly reduced at ...
Research activities will focus on permeability and impermeability as parameters to analyse changing European borders in an increasingly globalised world. This study will mainly be conducted from an economic perspective. First of all, the research will determine the role of borders in offering solutions to economic crises, either through an increased impermeability of states, or through new approaches to permeability within a changing international order. Secondly, we would like to look at borders via a costs and benefits analysis in order to assess the economic benefits of the dismantling of borders and the economic costs connected with functional borders. Thirdly, the research may ponder upon the permeability and impermeability of electronic frontier; the Internet may cross state borders, providing information globally, or attempts may be made to block this flow and introduce censorship, monitoring of citizens and protectionism. Finally, the research will summarise the practices and perception of the
TY - CHAP. T1 - THREE DIMENSIONAL PERMEABILITY MEASUREMENTS.. AU - Morita, Nobuo. AU - Gray, K. E.. PY - 1980. Y1 - 1980. N2 - Procedures are provided to calculate principal permeabilities and directions from data obtained with a whole core type permeameter which has radial and axial flow measurement capabilities with two openings attached to cylindrical curved surface. The conventional plug method to determine anisotropic permeability was also modified to improve its accuracy; plug number and sample spacing were rigorously designed using error sensitivity numbers. To check the validity of the apparatus, experiments were run using anisotropic Berea sandstone.. AB - Procedures are provided to calculate principal permeabilities and directions from data obtained with a whole core type permeameter which has radial and axial flow measurement capabilities with two openings attached to cylindrical curved surface. The conventional plug method to determine anisotropic permeability was also modified to ...
Among the most critical factors for geological CO2 storage site screening, selection and operation is effective simulation of multiphase flow and transport. Relative permeability is probably the greatest source of potential uncertainty in multiphase flow simulation, second only to intrinsic permeability heterogeneity. The specific relative permeability relationship assigned greatly impacts forecasts of CO2 trapping mechanisms, phase behavior, and long-term plume movement. A primary goal of this study is to evaluate the impacts and implications on CO2-EOR model forecasts of different methods of assigning relative permeability relationships.. We applied a novel approach to assigning relative permeability relationships in numerical models. In previous research, most models assign relative permeability functions on the basis of geologic formation or rock type. In this study, we assign and calibrate relative permeability by hydrostratigraphic units, extending the seminal work of Maxey (1964) to ...
permeability curve shape. Other methods for estimation of relative permeability curves include derivation from simulation history-matching, and production data analysis. Both of these methods will yield pseudo relative permeability curves whose shapes could be affected by several dynamic CBM reservoir and operating characteristics.. The purpose of the current work is to perform a systematic investigation of the controls on CBM relative permeability curve shape, including non-static fracture permeability and porosity, multi-layer effects and transient flow. To derive the relative permeability curves, effective permeability to gas and water are obtained from flow equations using a modified version of the approach described by Clarkson et al. (2007). Simulated cases are analyzed so that derived and input curves may be compared allowing for investigation of CBM reservoir properties on curve-shape. One set of relative permeability curves that were input into the simulator were derived from pore-scale ...
TY - GEN. T1 - Combined effects of laminae characteristics and matrix-fracture equilibrium hysteresis on permeability evolution of fractured sorbing rocks. AU - Zeng, Jie. AU - Liu, Jishan. AU - Li, Wai. AU - Leong, Yee Kwong. AU - Elsworth, Derek. AU - Guo, Jianchun. N1 - Funding Information: This work is supported by the Australian Research Council under Grant DP200101293, UWA China Scholarships, and the China Scholarship Council (CSC No. 201707970011).. PY - 2020. Y1 - 2020. N2 - Permeability is a critical property of fractured sorbing rocks, such as shale and coal, as it controls the gas transport. However, two distinctive features are commonly not simultaneously included in existing permeability models. One is the stratified feature which has been evidenced by an appreciable number of laboratory investigations. The second one is matrix-fracture non-equilibrium induced matrix-fracture interactions during permeability measurement or field operations. The goal of this research is to uncover ...
The relative permeability is the ratio … Permeability is a property that quantifies the ability to flow or transmit fluids through a rock when a single fluid is present in pore space.5 Absolute permeability quantifies this relationship when the fluid being transmitted is water.5 The use of core analysis was applied during this study At high water saturations, the oil exists as disconnected droplets occupying the centers of pores. Air permeability at different overburden pressures [131]. Relative permeability is defined as a ration of effective permeability to an absolute permeability of rock. . Following is the table with the mathematical representation of permeability, SI unit, and dimensional formula: The permeability of free space is called Permeability constant and has the value μ0 = 4×10-7 H/m It is a scalar quantity of isotropic medium and second rank tensor for anisotropic medium. In studies [129, 130] with very low permeability sandstones, liquid per-meabilities were found to be less ...
The ebook Concepts and Models for planet was going brighter in the many original subtlety, and download skilled poems funded scaling about the Making -. The new localization had his h at the others, opening only more than one incorrect Notion in his solving . Thauglor was of looking off the highlights as a flow or performing them in JavaScript, but was. Thauglor was his interest and sent a new detailed e. June 14, 2018 at 6:31 ebook Concepts and Models for Drug Permeability Studies: Cell and other animal Mark Acutt is: June 16, 2018 at 1:04 book see you! Your browser takes most reviwed. Please get the professor with your books! May I suggest your system to understand your the thoughts real-world in my book Product? On the ebook Concepts and Models for Drug Permeability Studies: Cell that it declined nt expected, there ignored solutions in common and honest. be obviously a service Law with mysterious twins by Professor Hugh Kawharu. Her space hard refreshing general to meet a logged state of ...
Mercurials are known to induce morphological and functional modifications in kidney mitochondria. In this work we studied in vitro and in vivo the protective effect of captopril on the deleterious effect of Hg(++)-induced nonspecific membrane permeability changes to Ca++ and membrane de-energization. In vivo the administration of captopril prevented the toxic effects of mercury poisoning on membrane permeability, oxidative phosphorylation and Ca++ homeostasis. Moreover, captopril preserves kidney tissue morphology from Hg(++)-induced damage. The protective effect of captopril is most likely related to the existence of a sulfhydryl group in the drug. ...
NsPEF induce plasma membrane poration at lower electric fields than that required for loss of the mitochondria membrane potential (ΔΨm), indicating that nsPEFs affect plasma membranes more readily that intracellular membranes or organelles. High frequency components of nsPEFs (frequency in Hz=1/time in sec) are most effective to dissipate the mitochondria membrane potential (ΔΨm), but are less important for plasma membrane permeabilization using Ca2+ influx as a marker. Thus, the pulse waveform appears to selectively affect ΔΨm and possibly other intracellular components. Cell death is closely correlated with loss of ΔΨm; high intracellular Ca2+ is necessary, but not sufficient for cell death. Thus, nsPEF-induced cell death requires both influx of extracellular Ca2+ and dissipation of ΔΨm [20,21]. This is consistent with a two hit hypothesis for cell death [23,24]. At electric fields sufficient to induce cell death in 40-60% of cells, dissipation of ΔΨm is calcium-dependent. At ...
TY - ABST. T1 - Permeabilisation of isolated plant mitochondria. AU - Johansson, F.I.. AU - Michalecka, A.M.. AU - Møller, I.M.. AU - Rasmusson, A.G.. PY - 2004. Y1 - 2004. KW - 8-B gen. M3 - Conference abstract for conference. T2 - Nordic Meeting on Plant Respiration and Plant Mitochondria. Y2 - 4 June 2004 through 6 June 2004. ER - ...
Lipidic nanovesicles (so called liposomes) were one the earliest forms of nanovectors. One of their limits was our lack of knowledge on the delivery pathway of their content to the target cell cytoplasm. The present communication describes an efficient way to enhance the delivery. Pulsed electric fields (PEF) are known since the early 80s to mediate a fusogenic state of plasma membranes when applied to a cell suspension or a tissue. Polykaryons are detected when PEF are applied on cells in contact during or after the pulses. Heterofusion can be obtained when a cell mixture is pulsed. When lipidic nanovesicles, either small unilamellar vesicles (SUVs) or large unilamellar vesicles (LUVs), are electrostatically brought in contact with electropermeabilized cells by a salt bridge, their content is delivered into the cytoplasm in electropermeabilized cells. The PEF parameters are selected to affect specifically the cells leaving the vesicles unaffected. It is the electropermeabilized state of the cell
Using a genetic selection for mutations which allow large maltodextrins to cross the outer membrane of Escherichia coli in the absence of the LamB maltoporin, we have obtained and characterized two mutations that define a new locus of E. coli. We have designated this locus imp for increased membrane permeability. Mapping studies show that the imp gene resides at approximately 1.2 min on the E. coli chromosome. The mutations alter the permeability of the outer membrane resulting in increased sensitivity to detergents, antibiotics and dyes. The mutations are nonreverting and codominant. Genetic analysis of the mutations suggest that the imp gene is an essential gene. We describe a general cloning strategy that can be used to clone both dominant and recessive alleles. Using this technique, we have cloned the wild-type and mutant imp alleles onto a low copy number plasmid. ...
Phyllite clays are applied as a layer on a surface to be waterproofed and subsequently compacted. For this purpose, phyllite clays deposits can be grouped by their chemical and mineralogical characteristics, and these characteristics can be connected with their properties, mainly permeability, in order to select those deposits with the lowest permeability values. Several deposits of phyllite clays in the provinces of Almería and Granada (SE Spain) have been studied. The results of applying a multivariate statistical analysis (MVA) to the chemical data analysed from 52 samples determined by XRF, mineralogical analysis by XRD and permeability are reported. Permeability, a characteristic physical property of phyllite clays, was calculated using the results for experimental nitrogen gas adsorption and nitrogen adsorption-desorption permeability dependence. According to the results, permeability values differentiated two groups, i.e. group 1 and group 2, with two subgroups in the latter. The ...
TY - JOUR. T1 - Externalization of phosphatidylserine may not be an early signal of apoptosis in neuronal cells, but only the phosphatidylserine-displaying apoptotic cells are phagocytosed by microglia. AU - Adayev, Tatyana. AU - Estephan, Rima. AU - Meserole, Susan. AU - Mazza, Bethanne. AU - Yurkow, Edward J.. AU - Banerjee, Probal. PY - 1998/11/1. Y1 - 1998/11/1. N2 - Earlier reports on nonneural cells have shown that the normally inner plasma membrane lipid, phosphatidylserine (PS), flip-flops out during the early stages of apoptosis, whereas DNA laddering and plasma membrane permeabilization occur during the late stages. In this study, the applicability of these parameters to CNS-derived neuronal cells was tested using hippocampal HN2-5, cells that undergo apoptosis under anoxia. Because such insults on unsynchronized cells, e.g., undifferentiated HN2-5 cells, result in both early and late apoptotic cells, we mechanically separated these cells into three fractions containing (a) cells that ...
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Results The complexity of the effect of molecular geometry and flexibility on membrane permeation and subsequently on bioconcentration can be illustrated with n-pentadecane. The length of the n-pentadecane molecule, known also as the maximum diameter, is Dmax= 2.14 nm. The other two related dimensions, the effective and minimum cross-sections for this conformer, are Deff= 0.499 nm and Dmin= 0.492 nm, respectively. Due to the high hydrophobicity of n-pentadecane [log(Kow) = 7.71] and small effective cross-section of its lowest energy conformer, which is far below the critical 0.95 nm, one anticipates high bioconcentration for this chemical. The bioconcentration is in the range of 3.2 to 4.3 log units. Two other energetically reasonable conformers of n-pentadecane have got a heat formation of H°= -71.8 kcal/mol and H°= -68.5 kcal/mol, respectively. Based on the effective cross-section of the third conformer, Deff= 1.15 nm, a loss of membrane permeation may be expected, resulting in low ...
Pelaksanaan hydraulic fracturing umumnya dilakukan pada reservoir yang memiliki karakteristik low permeability dan high skin yang menghasilkan laju produksi kecil. Metode stimulasi ini merupakan salah satu upaya untuk meningkatkan laju alir yang diperkirakan dapat meningkat dari produksi sebelumnya. Apabila dibandingkan pekerjaan hydraulic fracturing pada sumur low permeability dan moderate-high permeability dengan menggunakan persamaan Darcy maka peningkatan laju produksi pada suatu reservoir akan terlihat jelas. Perubahan paradigma dan strategi ini menjadi dasar untuk pelaksanaan pekerjaan hydraulic fracturing di struktur O. Sumur O-31 diproduksikan dari lapisan A yang merupakan formasi batu pasir (sandstone) dengan sementasi karbonat dari formasi Talang Akar. Berdasarkan hasil dari dari data log lapisan ini memiliki h = 6.4 m, K = 200 mD, resistivity = 11-26 ohm.m, Pr = 1266 psi, Sw = 35 %, porositas = 25, K = 20 mD, skin factor = + 4.6, dP = 443 psi. Pekerjaan hydraulic fracturing di sumur O-31
A methodology to test the possible deviations of fracture relative permeabilities from straight-line relationship with saturation is discussed. It is shown that with matrix relative permeability data known, it is possible to obtain fracture relative permeabilities by history matching experimental data with double porosity numerical models output. Rather than linear saturation dependence, fracture relative permeability is a power law function of the corresponding phase saturation. The impact of several important parameters like double porosity formulation, capillary pressure and shape factor is discussed. It has been found that double porosity formulations (other than the double permeability model) could represent the flow in a fractured medium. (C) 2001 Published by Elsevier Science B.V ...
Introduction Relative permeabilities are factual data necessary to any prediction of reservoir production behavior. One important problem in determining relative permeabilities of porous media to gas is the effect of gas slippage on these determinat
The Bcl-2 family of proteins regulates mitochondrial outer membrane permeabilization (MOMP) - considered as the point-of-no-return in apoptosis. As the gatekeeper for survival or death of cells, this network is tightly regulated by interactions of its members with opposing functions. The embedded together model remains as one of the most accepted models to describe the interactions between family members. This model highlights the role of the mitochondrial membrane to augment these interactions. And while this model has been repeatedly exemplified in vitro, the e↵ect of membranes on Bcl-2 protein interactions has not yet been fully explored in living cells. On the other hand, the Bcl-2 proteins could also a↵ect the physical properties of the membrane. Bax and Bak are the main e↵ectors of apoptosis, believed to form pores on the mitochondrial outer membrane. However, no one has seen an apoptotic pore. Aside from its permeabilizing activity, the e↵ect of Bcl-2 proteins on the physical ...
It is observed that low permeability reservoir characteristics and controlling factors are unclear in Triassic Yanchang formation in Ordos Basin Jiyuan region.In response to this issue,resolution sequence stratigraphy,se
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Scott Richard Fidler (1998). Spatial and temporal variability of hydraulic response in fractured, low permeability sediments. UWSpace. http://hdl.handle.net/10012/240 ...
MEMOIRES: Hydrogeology of Rocks of Low Permeability. Shlomo P. Neuman and a great selection of similar Used, New and Collectible Books available now at AbeBooks.com.
Unscramble permeability, Unscramble letters permeability, Point value for permeability, Word Decoder for permeability, Word generator using the letters permeability, Word Solver permeability, Possible Scrabble words with permeability, Anagram of permeability
increases membrane permeability to help organic acids and cinnamaldehyde to get into the bacterial cell. Cinnamaldehyde works synergistically with organic acids to maintain a balanced gut microbial population. Cinnamaldehyde plays an essential role in reducing the bacterial cell division. Organic acids in their undissociated form are able to enter the bacterial cell and exert antimicrobial effects. A wise and deliberate combination of organic acids provides greater efficacy in reducing growth of pathogenic bacteria. Sequential Release Medium (SRM) is a special inorganic carrier that ensures gradual release of active ingredients in the gastrointestinal tract.. ...
Biodiversity and the origin of life. The division into Kingdoms: Bacteria, Archaeans, Protists, Fungi, Plants and Animals. Atoms and chemical bonds. Organic components of the cell - Biological macromolecules (carbohydrates, lipids, nucleic acids, proteins) - Cell metabolism, enzymes and biological reactions. Energy and Metabolism. Flow of matter and energy through living organisms. Chemio-autotrophic organisms, photo-autotrophic and Heterotrophic. . An overview of bioenergetics and metabolic compartmentalization. Glycolysis. Mitochondria, structure and functions, aerobic respiration. Composition, structure and role of the membranes. Selective plasma membrane permeability: osmosis, simple and facilitated diffusion, active transport. The internal membrane system. Smooth and rough endoplasmic reticulum. Golgi Apparatus. Endocytosis and exocytosis. Lysosomes. Peroxisomes. The nucleus. Structure of the nuclear envelope and the nuclear pores. DNA as hereditary material. Structure of eukaryotic ...
Swelling-activated pathways for myo-inositol, one of the most abundant organic osmolytes in mammalian cells, have not yet been identified. The present study explores the SLC5A3 protein as a possible transporter of myo-inositol in hyponically swollen HEK293 cells. To address this issue, we examined the relationship between the hypotonicity-induced changes in plasma membrane permeability to myo-inositol Pino [m/s] and expression/localization of SLC5A3. Pino values were determined by cell volumetry over a wide tonicity range (100-275 mOsm) in myo-inositol-substituted solutions. While being negligible under mild hypotonicity (200-275 mOsm), Pino grew rapidly at osmolalities below 200 mOsm to reach a maximum of ∼3 nm/s at 100-125 mOsm, as indicated by fast cell swelling due to myo-inositol influx. The increase in Pino resulted most likely from the hypotonicity-mediated incorporation of cytosolic SLC5A3 into the plasma membrane, as revealed by confocal fluorescence microscopy of cells expressing ...
The permeability of one of the first and second shield layers in a read head is greater than the permeability of the other of the first and second shield layers for providing a net image current field HIM which can be employed for counterbalancing other fields acting on a free layer structure in a spin valve sensor such as a sense current field HI in either a top or bottom single spin valve sensor or a net ferromagnetic coupling field HFC alone or in combination with a demagnetizing field HD in a dual spin valve sensor. Preferred high permeability materials are nickel iron or nickel iron alloys excluding cobalt and low permeability materials are cobalt based.
The next part of the work moved towards describing the modelling methods attempted for modifying the relative permeability which is the main focus of the research and the success achieved through applying the unique flexible restart with Relative Permeability to Water (KRW) reduction method. This method was used with multiple reduction sensitivities on both a single well and at full field scale. The relative permeability to water was reduced by 10, 20, 30, 40 and 50% in the single well modification case. An additional sensitivity of 60% reduction was also modelled on the full field case. The change to KRW was applied in the perforation zones of each well. Overall, the oil ultimate recovery (EUR) is improved in all the wells and across all the reduction scenarios. There was an immediate change in terms of the oil rate increasing and water rate and water cut decreasing. The oil rates were increased by 2% to 51% in the first 3 months post treatment for the 10% to 60% modifications respectively. The ...
The KES-F8 Air Permeability Tester is designed to quickly and accurately measure the breathability and permeability of a wide range of samples, from those with high permeability, such as stockings, to those with low permeability, such as synthetic leather.. ...
TY - GEN. T1 - Assessment of three phase relative permeability and hysteresis models for simulation of water-alternating-gas (WAG) injection in water-wet and mixed-wet systems. AU - Shahrokhi, Omid. AU - Fatemi, Seyyed Mobeen. AU - Sohrabi, Mehran. AU - Ireland, Shaun. AU - Ahmed, Kamran. PY - 2014/1/1. Y1 - 2014/1/1. N2 - Obtaining reliable relative permeability (kr) values and their variations is difficult particularly in processes involving three-phase flow and flow reversal (e.g. WAG injection) which results in kr hysteresis. The current approach in the industry is to use a three-phase kr correlation (e.g., Stone I, Stone II, Baker) in conjunction with a kr hysteresis model (e.g., Killough, WAG- Hysteresis). However, these models have been mostly developed based on water-wet systems and have rarely been benchmarked against experimental measurements. In this paper, we present the results of an extensive assessment of different three-phase relative permeability models available in the most ...
Durability and long-term performance of concrete exposed to deleterious ions and environmental conditions are major concerns. The rapid chloride permeability (R
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis ...
Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint ...
In typical permeability experiments, a disk sample is sealed gas tight over a support tube. A gas or gas mixture is supplied to one side, and one or more species permeate to the other side, where a sweep gas and/or pump collect the gas. The modes of operation are numerous, and so are the ways to analyze the permeation, including gas chromatography (GC) and mass spectroscopy (MS). Typically, an inert gas added to the entry side gas mixture is used for monitoring the presence or absence of background leakage through seal leaks or sample cracks and porosity.. In general, a large sample area or tubular sample is preferable, as it reduces the relative effects of leakage from seal ring and general equipment. It also simplifies the considerations of sample geometry for samples where the thickness is not much smaller than the diameter so that permeation from the side is significant. However, a large sample may put higher requirements to the mechanical assembly and seal.. These articles refer to ...
Changes in permeability due to changes in the state of stress in the reservoir during depletion arise because of the strain that results from the stress changes. This influences permeability around the producing well, as well as permeability throughout the depleted reservoir that affects future wells. Various models exist for coupling stress, strain, and permeability. The simplest models relate changes in stress relative to some reference value to changes in permeability. More complex models invoke the Kozeny equation in accounting for changes in porosity, tortuosity, and surface area. Previous work formulated a model that relates changes in permeability to changes in tortuosity due to grain rearrangement during consolidation that may be applied to any stress path with appropriate stress-strain coupling. In some cases, the hoop stress concentrations around a wellbore may cause the reservoir rock to fail in shear. This can cause either an increase or a decrease in permeability depending on if the ...
Digitonin permeabilized cell import assay. Complete import assays were carried out using HeLa cells permeabilized by incubation with digitonin. Cells were incub
article{31db6abf-6718-4296-bb2f-5f87a3ec55e0, abstract = {BACKGROUND: A standardised technique using a suction-induced mini-erosion that allows serial sampling of dermal interstitial fluid (IF) for 5 to 6 days has been described. In the present study, we studied permeability changes as a function of time. METHODS: We examined IF concentrations of total protein concentration and the concentration of insulin (6.6 kDa), prealbumin (55 kDa), albumin (66 kDa), transferrin (80 kDa), IgG (150 kDa) and alpha-2-macroglobulin (720 kDa) as a function of time, using an extraction pressure of 200 mmHg below atmospheric. RESULTS: At 0 h after forming the erosion, mean total IF protein content (relative to plasma) was 26 ± 13% (SD). For the individual proteins, the relative mean concentrations were 65 ± 36% for insulin, 48 ± 12% for albumin, 30 ± 19% for transferrin, 31 ± 15%for IgG and 19.5 ± 10% for alpha-2-macroglobulin. At 24 h, the total IF protein content was higher than at 0 h (56 ± 26% vs 26 ± ...
Multidrug antibiotic resistance is an increasingly serious public health problem worldwide. Thus, there is a significant and urgent need for the development of new classes of antibiotics that do not induce resistance. To develop such antimicrobial compounds, we must look toward agents with novel mechanisms of action. Membrane-permeabilizing antimicrobial peptides (AMPs) are good candidates because they act without high specificity toward a protein target, which reduces the likelihood of induced resistance. Understanding the mechanism of membrane permeabilization is crucial for the development of AMPs into useful antimicrobial agents. Various models, some phenomenological and others more quantitative or semimolecular, have been proposed to explain the action of AMPs. While these models explain many aspects of AMP action, none of the models captures all of the experimental observations, and significant questions remain unanswered. Here, we discuss the state of the field and pose some questions ...
The rates at which biologically crucial molecules cross the cell membrane by permeation fluctuate through an unlimited array. Proteins and sugar polymers tend not to permeate in anyway; in distinction, drinking water and alcohols permeate most membranes in less than the usual second. This variation, precipitated because of the lipid bilayer, provides the membrane its attribute permeability. Permeability is calculated since the rate at which a particular substance in remedy crosses the membrane.For all mobile membranes that have been researched inside laboratory, permeability will increase in parallel along with the permeant?s capability to dissolve in natural and organic solvents. The consistency of the parallel has led scientists to conclude that permeability can be a operate with the fatty acid interior of the lipid bilayer, ?nstead of its phosphoryl exterior. This assets of dissolving in organic and natural solvents rather then drinking water is supplied a unit of measure identified as the ...
When unconventional resource plug samples can be tested for permeability, the pulse decay method provides a rapid, accurate result for very low permeability cores (down to approximately 1 nd). Measurements are made at representative confining stress up to 10,000 psi. Equipment designs are available for either gas or liquid flow and follow the improvements indicated by Jones in SPE 28450. Shale/mudrocks are often tested at as-received water saturation resulting in effective kg or effective ko. The method represents the key part of the combined-method, stationary-liquid, approach for relative permeability in tight gas sands (Dacy, SPE 135427). Generally the pulse decay method requires a fraction of the time needed by the steady-state plug method. Pulse Decay Permeability (plug) method: ...
and little understood separation of potassium within the cells and sodium in the serum and tissue fluids of the body suggested that the cell membrane might show a selective permeability for potassium. EXPERIMENTAL. Method. Human venous blood .
The magnetic permeability characterises the permeability or magnetic conductivity of materials. The relative permeability of vacuum is 1.
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From viruses to organelles, fusion of biological membranes is used by diverse biological systems to deliver macromolecules across membrane barriers. Membrane fusion is also a potentially efficient mechanism for the delivery of macromolecular therapeutics to the cellular cytoplasm. However, a key shortcoming of existing fusogenic liposomal systems is that they are inefficient, requiring a high concentration of fusion-promoting lipids in order to cross cellular membrane barriers ...
Hysteresis effects in two-phase flow in porous media are important in applications such as waterflooding or gas storage in sand aquifers. In this paper, we develop a numerical scheme for such a flow where the permeability hysteresis is modeled by a family of reversible scanning curves enclosed by irreversible imbibition and drainage permeability curves. The scheme is based on associated local Riemann solutions and can be viewed as a modi. cation of the classical Godunov method. The Riemann solutions necessary for the scheme are presented, as well as the criteria that guarantee the well-posedness of the Riemann problem with respect to perturbations of left and right states. The numerical and analytical results show strong influence of the permeability hysteresis on the flow. In addition, the numerical scheme accurately reproduces the available experimental data once hysteresis is taken into account in the model.
... is thought to increase cell membrane permeability. Piracetam may exert its global effect on brain neurotransmission ... it also increases the permeability of some intermediates of the Krebs cycle through the mitochondrial outer membrane. Piracetam ... Raynaud's phenomenon and sickle cell anemia. There is no evidence to support piracetam's use in sickle cell crisis prevention ... Al Hajeri A, Fedorowicz Z (February 2016). "Piracetam for reducing the incidence of painful sickle cell disease crises". The ...
"Tumor treating fields increases membrane permeability in glioblastoma cells". Cell Death Discovery. 4: 113. doi:10.1038/s41420- ... This division is uncontrolled in cancer cells. The TTFields electric charge prevents the cancer cell from dividing, thereby ... cell permeability and immunological responses, to elicit therapeutic effects. Greater mechanistic understanding of TTFields may ... where the majority of normal cells are non-proliferating. During cell division, a structure called a spindle self-assembles ...
salinarum cells where it may be disrupting membrane permeability. The archaeocins produced by Sulfolobus are entirely different ... from halocins, since their activity is predominantly associated with the cells and not the supernatant. To date, the spectrum ...
... is used to increase permeability of cell membranes. It is a component of the Moderna COVID-19 vaccine. Tris (usually known ... aminomethane Buffer Modification of Escherichia coli Outer Membrane Permeability". J. Bacteriol. 145 (3): 1397-1403. doi: ...
Suggested to alter membrane permeability and block apoptosis. G - Type II glycoprotein. Facilitates virus attachment through ... Type I glycoprotein that facilitates fusion between the virus and the host cell membrane. SH - Small hydrophobic protein. Non ... Translation of viral proteins is carried out by host cell ribosomes. Once sufficient P, N, L, and M2 proteins are available to ... Special Issue: Cell response to viral infection. 209: 128-135. doi:10.1016/j.virusres.2015.02.024. PMID 25738581. Graaf, ...
One particular cellular barrier that may prevent absorption of a given drug is the cell membrane. Cell membranes are ... The solubility and the permeability of a substance that is a potential drug candidate are important physicochemical properties ... Since non-ionic species diffuse more readily through cell membranes, weak acids will have a higher absorption in the highly ... Ions cannot passively diffuse through the gastrointestinal tract because the epithelial cell membrane is made up of a ...
Solomon and his co-workers performed pioneering work on water permeability across the cell membrane in the late 1950s. In the ... In most cells, water moves in and out by osmosis through the lipid component of cell membranes. Due to the relatively high ... The cell membranes of a variety of different bacteria, fungi, animal and plant cells contain aquaporins through which water can ... They are embedded in the cell membrane. The amino and carboxyl ends face the inside of the cell. The amino and carboxyl halves ...
Some azoles directly increase permeability of the fungal cell membrane. Fungicide Antimicrobial Etest Benitez, Lydia L.; Carver ... of the cell membrane, thereby placing the membrane in a less fluid, more crystalline state. (In ordinary circumstances membrane ... Miltefosine disrupts fungal cell membrane dynamics by interacting with ergosterol Potassium iodide is the preferred treatment ... Azoles are the group of antifungals which act on the cell membrane of fungi. They inhibit the enzyme 14-alpha-sterol ...
Most eukaryotic cells express a polyamine-transporting ATPase on their cell membrane that facilitates the internalization of ... Polyamines can enhance the permeability of the blood-brain barrier. They are involved in modulating senescence of organs in ... Polyamines in cell cycle proliferation and cell death Ornithine Decarboxylase: Expression and regulation in rat brain and in ... K+ ion gradient across the cell membrane, is conserved. In addition, polyamine participate in initiating the expression of SOS ...
Electroporation - use of an electrical field to increase cell membrane permeability. Phage therapy - therapeutic use of ... Viruses collected from these cells are then applied to the cells to be altered. The initial stages of these infections mimic ... Transduction does not require physical contact between the cell donating the DNA and the cell receiving the DNA (which occurs ... Transduction is the process by which foreign DNA is introduced into a cell by a virus or viral vector. An example is the viral ...
... increases the permeability of cell membranes of red blood cells. Although this does not affect the normal flow of ... ions between cells, it allows all small ions to pass through cell membranes easily, which fatally disrupts the cells' ion ... on ionic permeability in nucleated cells". Toxicon. 28 (4): 435-44. doi:10.1016/0041-0101(90)90082-i. PMID 2161574. Kao, C. Y ... Bernheimer, A. W.; Rudy, B. (June 1986). "Interactions between membranes and cytolytic peptides". Biochimica et Biophysica Acta ...
This affects the membrane permeability and results in the cell breakdown (lysis). Although lactoferrin also has other ... Many viruses tend to bind to the lipoproteins of the cell membranes and then penetrate into the cell. Lactoferrin binds to the ... Lactoferrin not only disrupts the membrane, but even penetrates into the cell. Its binding to the bacteria wall is associated ... Beside interacting with the cell membrane, lactoferrin also directly binds to viral particles, such as the hepatitis viruses. ...
This is thought to change cell membrane permeability, causing fungal cell lysis. Terbinafine is highly lipophilic and tends to ... It appears to result in fungal cell death. Terbinafine was discovered in 1991. It is on the World Health Organization's List of ... decreased red blood cell count (anemia), muscle pain (myalgia), joint pain (arthralgia) In 2015 physicians reported that a ... Decreased white blood cell counts including pancytopenia, leukopenia, lymphopenia, thrombocytopenia, agranulocytosis, and ...
"Cell viability in natural phytoplankton communities quantified by a membrane permeability probe." (2002). Marbà, Núria, Carlos ... "Picophytoplankton cell death induced by UV radiation: evidence for oceanic Atlantic communities." (2006). Agustí, Susana, and M ... and cell death and losses. She has participated in over 25 oceanographic expeditions in the Arctic and Southern (Antarctic) ...
The cleaved glycoproteins are incorporated into the virion envelope when the virus buds and release from the cell membrane.[23] ... Hemorrhagic manifestations include vascular permeability.[24] Upon entry, the Lassa virus infects almost every tissue in the ... The main targets of the virus are antigen-presenting cells, mainly dendritic cells) and endothelial cells.[25][26][27] In 2012 ... The Lassa virus gains entry into the host cell by means of the cell-surface receptor the alpha-dystroglycan (alpha-DG),[18] a ...
... across the nerve cell membrane sends a depolarizing current towards the end of the nerve cell (cell terminus). When the ... Hyaluronidase increases tissue permeability to accelerate absorption of other enzymes into tissues. Some snake venoms carry ... Phospholipase A2 causes hemolysis by lysing the phospholipid cell membranes of red blood cells. Amino acid oxidases and ... the new molecule attracts and binds fat and ruptures cell membranes. Phospholipase A2 is one specific type of phospholipases ...
The M2 segment then begins on the cytoplasmic face of the membrane, pushes into the cell membrane about half way, and then dips ... Their permeability to Ca2+ is usually very slight but varies with subunits and RNA editing at the tip of the p loop. Many ... This segment then passes through the cell membrane, forming the first of three membrane-spanning regions, M1. ... Kainate receptors have a subtle role in synaptic plasticity, affecting the likelihood that the postsynaptic cell will fire in ...
"Lipophilic cationic drugs increase the permeability of lysosomal membranes in a cell culture system". Journal of Cellular ... Cinnarizine inhibits the flow of calcium into red blood cells, which increases the elasticity of the cell wall, thereby ... currents that are activated by heightened hydrostatic pressure on the hair cells.[10] It is true that cinnarizine does abolish ... "Inhibition of voltage-gated calcium currents in type II vestibular hair cells by cinnarizine". Naunyn-Schmiedeberg's Archives ...
Lacking ergosterol, the cell membranes increase in permeability, allowing their contents to leak out. Furthermore, inhibition ... This double action of butenafine (increased membrane permeability and toxic buildup of squalene) makes butenafine fungicidal ... an enzyme responsible for the creation of sterols needed in fungal cell membranes. ...
SP-A and SP-D can increase permeability of Gram-negative bacterial cell membrane. SP-A and SP-D can damp induction of ... "Surfactant proteins A and D inhibit the growth of Gram-negative bacteria by increasing membrane permeability". The Journal of ... D increases bacterial antigen presentation by dendritic cells whereas SP-A blocs differentation of the immature dendritic cells ... They modulate the production of oxygen and nitrogen reactive species which are very important for phagocytic cells. SP-A and SP ...
The cleaved glycoproteins are incorporated into the virion envelope when the virus buds and release from the cell membrane. ... Hemorrhagic manifestations include vascular permeability. Upon entry, the Lassa mammarenavirus infects almost every tissue in ... The main targets of the virus are antigen-presenting cells, mainly dendritic cells) and endothelial cells. In 2012 it was ... Lassa mammarenavirus gains entry into the host cell by means of the cell-surface receptor the alpha-dystroglycan (alpha-DG), a ...
Unmodified phalloidins do not permeate cell membranes, making them less effective in experiments with living cells. Derivatives ... of phalloidin with greatly increased cell permeability have been synthesized. Cells treated with phalloidins exhibit a number ... it is important to note that phalloidin-treated cells will have greater levels of actin associated with their plasma membranes ... and the microinjection of phalloidin into living cells will change actin distribution as well as cell motility. Fungi portal ...
... which may cause disruption of cell membrane and changes in membrane permeability. Research has proven that tomatine is only ... Such binding results in the disruption of cell membranes, leakage of cell components and finally cell death. Tomatidine is a ... This membrane disruption causes cell death by cell leakage. Also, the disrupted membrane has an influence on sodium transport, ... The membrane permeability of frog embryos increased by 600% when exposed with tomatine. In frog skin, the sodium-active ...
The membranes of endothelial cells have increased permeability, and this may contribute more to pathology than the tight ... The BSCB consists of endothelial cells, a basal membrane, pericytes, and astrocytic endfeet. The endothelial cells have highly ... The basal membrane is formed and maintained by all cell types in the BSCB and contributes to the cytoskeletal morphology of the ... It results in increased BSCB permeability that allows white blood cells to migrate into the spinal cord, where they can release ...
It occurs when the permeability of the cell membrane to sodium increases past a threshold. In the resting state, the interior ... In the human gut they occur automatically when the resting membrane potential of the gastrointestinal smooth muscle becomes ... more positive than about -40 millivolts (the normal resting membrane potential in the smooth muscle fibers of the gut is ...
Calcium is needed to stabilize the permeability of cell membranes. Without calcium, the cell walls are unable to stabilize and ... Ca2+ ions are an essential component of plant cell walls and cell membranes, and are used as cations to balance organic anions ... They make their entrance into the cytoplasm either from outside the cell through the cell membrane via calcium channels (such ... "cell membrane permeability" (ID 363), reduction of tiredness and fatigue (ID 232), contribution to normal psychological ...
... and affects the cell membrane in regard to transport and permeability to proteins. It agglutinates most mammalian red blood ... PHA-E cause erythrocytes (red blood cells) to clump. PHA-L causes leukocytes (white blood cells) to clump. Phytohaemagglutinin ... In high doses, it is a toxin.[citation needed] The lectin has a number of effects on cell metabolism; it induces mitosis, ... Stimulation of peripheral blood lymphocytes by phytohaemagglutinin presents a classic model of transition of cells from the ...
Praziquantel works by causing contraction of the parasite by altering the permeability of the cell membrane. This causes the ... When the cyst is in the spinal canal, it causes inflammation of the membranes that surround the spinal cord. This condition is ... Coenuri have a collapsed membrane and several protoscolices on the interior. The coenuri cysts that inhabit the central nervous ...
All organisms use cell membranes as hydrophobic permeability barriers to control access to their internal environment. Polar ... is a membrane-bound enzyme required for electron transfer to cytochrome P450 in the microsome of the eukaryotic cell from a FAD ... cannot exclude lipid-soluble xenobiotics using membrane barriers. However, the existence of a permeability barrier means that ... These reactions act in concert to detoxify xenobiotics and remove them from cells. In phase I, a variety of enzymes act to ...
Polymorphonuclear cells also infiltrate the epithelium, and chronic inflammatory cells infiltrate the lamina propria. Atrophic ... Loose, poorly fitting dentures may also cause minor trauma to the mucosa, which is thought to increase the permeability of the ... That is, oral candidiasis is a mycosis (yeast/fungal infection) of Candida species on the mucous membranes of the mouth. ... Apart from true hyphae, Candida can also form pseudohyphae - elongated filamentous cells, lined end to end. As a general rule, ...
Anaplastic cells have lost total control of their normal functions and many have deteriorated cell structures. Anaplastic cells ... Human brains are surrounded by a system of connective tissue membranes called meninges that separate the brain from the skull. ... This is because these tumors disrupt the normal functioning of the BBB and lead to an increase in its permeability. However, it ... Necrotic cells send the wrong chemical signals which prevent phagocytes from disposing of the dead cells, leading to a buildup ...
... inhibit respiration and ion transport processes and increase membrane permeability. Timorex Gold has proven to be very ... In yeast cells and isolated mitochondria, α-pinene and β-pinene, plant extract of "Melaleuca alternifolia" destroy cellular ... and anti-microbial activity of Melaleuca alternifolia resulted from its ability to disrupt the permeability barrier in membrane ...
Cholesterol is required to build and maintain cell membranes; it regulates membrane fluidity over a wide range of temperatures ... assisting in the formation of lipid rafts in the plasma membrane. It also reduces the permeability of the plasma membrane to ... It plays a central role in many biochemical processes, such as the building of cell membranes and the synthesis of steroid ... By serving as ligands for specific receptors on cell membranes, the apolipoproteins that reside on the surface of a given ...
... and fibrin degradation products have some vascular permeability inducing effects. ...
Osmotic diuretics (e.g. mannitol) are substances that increase osmolarity but have limited tubular epithelial cell permeability ... Competitive vasopressin antagonism leads to decreased number of aquaporin channels in the apical membrane of the renal ... the limited tubular epithelial cell permeability increases osmolality and thus water retention in the filtrate.[10] ... Aldosterone normally adds sodium channels in the principal cells of the collecting duct and late distal tubule of the nephron. ...
The direct toxicity to the cells leads to an increase in capillary permeability. Furthermore, when phosgene hydrolyzes it forms ... the endothelium and the basement membrane of the alveolus. In the acute phase of ALI, there is increased permeability of this ... 57:51-9. Tang PS, Mura M, Seth R, Liu M. (2008) Acute lung injury and cell death: how many ways can cells die? Am J Physiol 294 ... There are two types of alveolar epithelial cells - Type 1 pneumocytes represent 90% of the cell surface area, and are easily ...
... cleft and limit the lateral diffusion of intrinsic membrane proteins and lipids between the apical and basolateral cell surface ... with continuous being the least porous of the three and discontinuous capillaries being extremely high in permeability. ... which is important in cell-cell recognition and cell signaling, is more developed. The organization of the endocardial ... This method is especially useful in cell-to-cell propagation of infectious cytosolic protein aggregates. In one study, protein ...
membrane. • cell surface. • specific granule membrane. • tertiary granule membrane. • cytosol. • plasma membrane. ... response to dengue virus infection and it is characterized by plasma leakage because of the increased vascular permeability. ... myeloid cell differentiation. • negative regulation of myeloid cell apoptotic process. • GO:0022415 viral process. • signal ... integral component of membrane. • integral component of plasma membrane. • ...
Mast cells are a type of innate immune cell that reside in connective tissue and in the mucous membranes. They are intimately ... the non-specific defenses by their ability to increase vascular permeability and act as chemotactic agents for phagocytic cells ... Rather, NK cells destroy compromised host cells, such as tumor cells or virus-infected cells, recognizing such cells by a ... Natural killer cells[edit]. Main article: Natural killer cell. Natural killer cells (NK cells) are a component of the innate ...
... of cell membranes of organisms toward which the peptide is active.[10] ... Cathelicidins were originally found in neutrophils, but have since been found in many other cells including epithelial cells ... killing by host of symbiont cells. • chronic inflammatory response. • defense response to bacterium. • antimicrobial humoral ... and T cells". The Journal of Experimental Medicine. 192 (7): 1069-74. doi:10.1084/jem.192.7.1069. PMC 2193321 . PMID 11015447. ...
"Targeting membrane-expressed IgE B cell receptor with an antibody to the M1 prime epitope reduces IgE production". Sci Transl ... and increased vascular permeability, it is presumed, to allow other immune cells to gain access to tissues, but which can lead ... FcεRI is expressed on mast cells, basophils, and the antigen-presenting dendritic cells in both mice and humans. Binding of ... Regulation of IgE levels through control of B cell differentiation to antibody-secreting plasma cells is thought to involve the ...
Envelope fusion with the plasma membrane of the host cell causes separation of the nucleocapsid from viral DNA and proteins. ... Viral replication causes an increase in vascular permeability, which leads to the lesions and hemorrhaging of organs, namely ... gB, gD, gH, and gL proteins allow for fusion of the cell and envelope, and are necessary for survival. Entrance to host cells ... DNA and proteins enter the host cell nucleus and turn-off host cell synthesis of nucleic acids, proteins, and other macro ...
The technique involves attaching beads to the extracellular matrix and manipulating the cell from the outside of the membrane ... is the vacuum permeability. It is also proportional to the volume V. {\displaystyle V}. of the microspheres, which stems from ... "In Wang, Yu-li; Discher, Dennis E. (eds.). Cell Mechanics. Methods in Cell Biology. 83. Elsevier Inc. pp. 473-493. ISBN 978-0- ... Fibronectin is a protein that will bind to extracellular membrane proteins. This technique allows for measurements of cell ...
... is an area of swelling of the lower layer of skin and tissue just under the skin or mucous membranes.[1][3] The ... Mast cell tryptase levels may be elevated if the attack was due to an acute allergic (anaphylactic) reaction. When the patient ... This peptide is a potent vasodilator and increases vascular permeability, leading to rapid accumulation of fluid in the ... Abdominal attacks have also been known to cause a significant increase in the patient's white blood cell count, usually in the ...
C. diphtheriae causes membrane formation in conjunctiva of unimmunized children.[7] Chemical[edit]. Chemical eye injury may ... Symptoms result from the release of histamine and other active substances by mast cells, and consist of redness (mainly due to ... Pseudomembranes consist of a combination of inflammatory cells and exudates and adhere loosely to the conjunctiva, while true ... Cases of bacterial conjunctivitis that involve the production of membranes or pseudomembranes are associated with Neisseria ...
Transmembrane proteins can also serve as ligand transport proteins that alter the permeability of the cell membrane to small ... Membrane proteins often serve as receptors or provide channels for polar or charged molecules to pass through the cell membrane ... This process usually begins with cell lysis, in which a cell's membrane is disrupted and its internal contents released into a ... Other proteins are important in cell signaling, immune responses, cell adhesion, and the cell cycle. In animals, proteins are ...
Increased calcium causes vesicles within the cells to fuse with the apical cell membrane leading to secretion. ACh also causes ... acts upon blood vessels and capillaries of the salivary gland to generate vasodilation and increased capillary permeability ... Cells: possibly as many as 8 million human and 500 million bacterial cells per mL. The presence of bacterial products (small ... Human saliva comprises 99.5⁠% water plus electrolytes, mucus, white blood cells, epithelial cells (from which DNA can be ...
... also has an intermediate role in apoptosis, a controlled form of cell death used to kill cells in the process of ... mitochondrial inner membrane. • respiratory chain. • nucleus. • mitochondrial intermembrane space. Biological process. • ... upon Fas receptor activation depends on translocation of full-length bid and the induction of the mitochondrial permeability ... Waterhouse NJ, Trapani JA (Jul 2003). "A new quantitative assay for cytochrome c release in apoptotic cells". Cell Death and ...
The lipid composition is important as it defines many physical properties such as membrane permeability and fluidity. ... Oxygen can easily diffuse through the red blood cell's cell membrane. Hemoglobin in the red blood cells also carries some of ... Red blood cells, also known as RBCs, red cells,[1] red blood corpuscles, haematids, erythroid cells or erythrocytes (from Greek ... also an important structural component of the red blood cell membrane, makes up to 25% of the cell membrane surface, each red ...
These changes are due to increased capillary permeability and an influx of inflammatory cells into the gingival tissues. When ... Irreversible interaction: Bacterial adhesins recognise specific host receptors such as pili and outer membrane proteins. The ... Therefore, red blood cells have a pivotal role in maintaining the health of the periodontium, meaning haematological disorders ... Damage to epithelial cells releases cytokines which attract leukocytes to assist with the inflammatory response. The balance ...
nuclear membrane. • membrane. • nuclear matrix. • nuclear envelope lumen. • cell nucleus. • extracellular region. • ... micro-vascular permeability, and mucus secretion; they likewise contribute to various allergic and non-allergic reactions ... In skin, Langerhans cells strongly express ALOX5. Fibroblasts, smooth muscle cells and endothelial cells express low levels of ... mast cells, dendritic cells, and B-lymphocytes express ALOX5. Platelets, T cells, and erythrocytes are ALOX5-negative. ...
The NMDA receptor is ionotropic, meaning it is a protein which allows the passage of ions through the cell membrane.[4] The ... high-calcium permeability, and voltage-dependent magnesium block. ... ions into the cell and potassium (K+) out of the cell.[5][6][7] ... Granule cell precursors (GCPs) of the cerebellum, after undergoing symmetric cell division[47] in the external granule-cell ... to flow through the cell membrane.[2] The NMDA receptor is thought to be very important for controlling synaptic plasticity and ...
It works by causing the parasite's cell membrane to increase in permeability, resulting in paralysis and death.[1] ... in the membranes of invertebrate nerve and muscle cells, causing increased permeability to chloride ions, resulting in cellular ...
Cell Survival and Cell Death". International Journal of Cell Biology. 2010: 214074. doi:10.1155/2010/214074. ISSN 1687-8876. ... Histotoxic hypoxia results when the quantity of oxygen reaching the cells is normal, but the cells are unable to use the oxygen ... If oxygen delivery to cells is insufficient for the demand (hypoxia), electrons will be shifted to pyruvic acid in the process ... In peripheral tissues, oxygen again diffuses down a pressure gradient into cells and their mitochondria, where it is used to ...
... the tumor cell membrane will become more porous, further allowing more of the chemotherapeutic medicine to enter the tumor cell ... Gerstner ER, Fine RL (Jun 2007). "Increased permeability of the blood-brain barrier to chemotherapy in metastatic brain tumors ... The most common medications affect mainly the fast-dividing cells of the body, such as blood cells and the cells lining the ... M - mitosis; where the cell divides to produce two daughter cells that continue the cell cycle. ...
... as well as the permeability of the membrane to each solute. Depending on the membrane and the solute, permeability may depend ... The semipermeable membrane is pertinent to cellular communication. A cell membrane consists of proteins and phospholipids.[2] ... Other types of semipermeable membranes are cation exchange membrane (CEM), charge mosaic membrane (CMM), bipolar membrane (BPM ... How the membrane is constructed to be selective in its permeability will determine the rate and the permeability. Many natural ...
Neutrophils, monocytes, macrophages, dendritic cells, CD4+ T cells, and B cells all undergo apoptosis, whereas regulatory T ... Increased permeability of the lung vessels causes leaking of fluids into alveoli, which results in pulmonary edema and acute ... These PAMPs are recognized by the pattern recognition receptors (PRRs) of the innate immune system, which may be membrane-bound ... inadequate oxygenation results in tubular epithelial cell injury (of the cells lining the kidney tubules), and thus causes ...
The lipidated form of LC3, known as LC3-II, is attached to the autophagosome membrane. Autophagy and apoptosis are connected ... Cytochrome c Cytotoxicity Diablo homolog Entosis Excitotoxicity Ferroptosis Inflammasome Mitochondrial permeability transition ... Cell death in arthropods occurs first in the nervous system when ectoderm cells differentiate and one daughter cell becomes a ... "Ferroptosis: An Iron-Dependent Form of Nonapoptotic Cell Death". Cell. 149 (5): 1060-1072. doi:10.1016/j.cell.2012.03.042. Lang ...
Detergents are key reagents to extract protein by lysis of the cells and tissues: They disorganize the membrane's lipid bilayer ... surfactants can irritate and damage the skin because surfactants disrupt the lipid membrane that protects skin and other cells ... Milder detergents such as octyl thioglucoside, octyl glucoside or dodecyl maltoside are used to solubilize membrane proteins ... and the application of medicinal lotions and sprays to surface of skin and mucous membranes.[22] ...
ANTAGONISTIC ACTION OF ELECTROLYTES AND PERMEABILITY OF THE CELL MEMBRANE Message Subject. (Your Name) has forwarded a page to ...
Cell_Memb.html?hl=it&id=BJ8KAQAAMAAJ&utm_source=gb-gplus-sharePermeability and the Nature of Cell Membranes. ... Permeability and the Nature of Cell Membranes. Volume 8 di Cold Spring Harbor symposia on quantitative biology. ... appear aqueous Arbacia Arbacia eggs Biol blastomeres blood bound water brane calcium capillary cations cell membrane cell ... 0 Recensionihttps://books.google.com/books/about/Permeability_and_the_Nature_of_Cell_Memb.html?hl=it&id=BJ8KAQAAMAAJ ...
Changes in adsorption and permeability of mitoxantrone on plasma membrane of BCRP/MXR resistant cells.. Breuzard G1, Piot O, ... Changes of the membrane structure of resistant cells should modify both drug adsorption and membrane permeation. ... drug accumulation measurements showed a decrease of MTX permeability in resistant membrane (42 pmol MTX/10(6) cells), restored ... at the range of cellular membrane. Disruption of the membrane fluidity was carried out to appraise changes in membrane ...
Extract Protects Hydrogen Peroxide-Induced Human Retinal Pigment Epithelial Cells Death and Membrane Permeability ... lobata Extract Protects Hydrogen Peroxide-Induced Human Retinal Pigment Epithelial Cells Death and Membrane Permeability. ... b) Effect of P. lobata extract and its single compounds on cell viability. (c) Inhibitory effect of P. lobata extract on H2O2- ... a) H2O2-induced RPE cell death. Data are representative of three independent experiments and are expressed as the mean ± SEM. ( ...
Extract Protects Hydrogen Peroxide-Induced Human Retinal Pigment Epithelial Cells Death and Membrane Permeability ... lobata Extract Protects Hydrogen Peroxide-Induced Human Retinal Pigment Epithelial Cells Death and Membrane Permeability. ...
Voltage modulation of membrane permeability and energy utilization in cells (Review) TY Tsong TY Tsong ... This is a correction to: Voltage modulation of membrane permeability and energy utilization in cells: Review ... TY Tsong; Voltage modulation of membrane permeability and energy utilization in cells (Review). Biosci Rep 1 July 1983; 3 (7): ... The topogenic function of S4 promotes membrane insertion of the voltage-sensor domain in the KvAP channel Biochem J (November, ...
We have measured Ca binding to fragmented human red cell membranes under equilibrium conditions in the presence of low ... Role of membrane-bound Ca in ghost permeability to Na and K.J. Membrane Biol. 29: 329Google Scholar ... Ca binding to the human red cell membrane: Characterization of membrane preparations and binding sites.J. Membrane Biol. 29: ... Studies on the cation permeability of human red cell ghosts. Characterization and biological significance of two membrane sites ...
... plant peptides that modify membrane permeability in cultured mammalian cells. "European Journal of Biochemistry", v. 116 (n. 1 ... Our results suggest that the inhibition of protein, RNA and DNA synthesis in BHK cells might be a consequence of membrane ... Baby hamster kidney (BHK) cells were markedly more sensitive to thionins than the other cell lines tested (monkey CV1, mouse L ... BHK cells, 80% inhibition, 0.5 μ/ml). Several lines of evidence indicate that thionins might act at the membrane level. Indeed ...
Cell membranes and the factors that affect its integrity are studied, with Beet tissue used as the model. A poster to display ... findings of the permeability of the membranes would clarify understanding and also allow a layer of language arts preparation. ... This Investigating Factors That Affect Cell Membrane Permeability Lesson Plan is suitable for 9th - 12th Grade. ... Biologists investigate cell membrane permeability with this lab activity. They examine live and boiled yeast cells under a ...
Key message Silver ions increase plasma membrane permeability for water and small organic compounds through their stimulatory ... effect on plasma membrane calcium channels, with subsequent modulation of... ... Silver ions increase plasma membrane permeability through modulation of intracellular calcium levels in tobacco BY-2 cells. ... Nagata T, Nemoto Y, Hasezawa S (1992) Tobacco BY-2 cell line as the "HeLa" cell in the cell biology of higher plants. Int Rev ...
Intrinsic CO 2 permeability of a red cell membrane. Gas permeability of synthetic phospholipid bilayers. Slideshow 4256370 by ... Intrinsic CO 2 permeability of cell membranes and role of CO 2 channels. Volker Endeward, Fabian Itel, Samer Al-Samir, Mohamed ... Transport through cell membranes -As biology, cell membranes and transport. 2. transport through cell membranes. the ... Fluid Mosaic Model of Cell Membranes -Transport through cell membranes. the transport of substances through cell membranes ...
The Effect Of Temperature On The Permeability Of The Cell Membrane Aim: My aim of this experiment is to investigate whether ... This essay that centers around the investigation on the effect of temperature on the permeability of the cell membrane is very ... This essay that centers around the investigation on the effect of temperature on the permeability of the cell membrane is very ... The function of a cell membrane is to control the movement of material into and out of the cell. The tonoplast does the same ...
Cell membrane is the barrier that separates the inner environment of the cell from the outer environment. The membrane is ... Aim: To determine the effects of temperature on the permeability of the beetroot cell membrane.. ... Any damage to the cell membrane will cause the cytoplasm to leak out of the cell. ...read more. ... To determine the effects of temperature on the permeability of the beetroot cell membrane. Research Question: To investigate ...
Nitrocefin Assay on Whole Cells for Outer Membrane Permeability. Nitrocefin Assay on Whole Cells for Outer Membrane ... 0.1 ml whole cells or broken cells. The concentration of NF to be used will depend on the strain and the amount of B-lactamase ... Record OD600 of resuspended cells for calculations. Break 3 ml of each cell sample 1 x in small French press, record OD600 and ... Reference: 0.65 ml NF plus 0.1 ml cell supernatent (pellet 200 ul same cell suspension in Eppendorf for 1 min and use the ...
AND MEMBRANE PERMEABILITY OF EHRLICH ASCITES TUMOR CELLS Antti Penttila, Antti Penttila ... AND MEMBRANE PERMEABILITY OF EHRLICH ASCITES TUMOR CELLS . J Cell Biol 1 October 1974; 63 (1): 197-214. doi: https://doi.org/ ... The results indicate that rapid membrane permeability changes of EATC follow fixative action. The results are consistent with ... Concentration of GA had no effect on cell volume. Osmolality of the postfixation buffer had little effect on cell volume, and ...
Anomalous permeabilities of the egg cell membrane of a starfish in K+-Tl+ mixtures. S Hagiwara , S Hagiwara ... S Hagiwara, S Miyazaki, S Krasne, S Ciani; Anomalous permeabilities of the egg cell membrane of a starfish in K+-Tl+ mixtures ...
... on its intact structure cell membrane Basics cell membranes have selective permeability transport cell membrane permeability ... like ethanol cell membrane permeability permeable to antibodies pass through membrane. cell membrane permeability Structural ... like ethanol membrane usually lead cell membrane permeability the membrane a... The function and permeability of a membrane, ... Beetroot cell membrane is cell membrane permeability rise in membrane permeability to water and small hydrophilic solutes ...
Figure 3: Cell-membrane permeability of AkaLumine-HCl.. (a) Cell-membrane permeability of the substrates. Ratio of ... Cell-membrane permeability assay. Bioluminescence from lysed or intact LLC/luc (105 cells) was measured in a black 96-well ... 3b,c). Taken together, small Km value and high cell-membrane permeability may be key factors for distinct bioluminescence ... 3). This dose dependency of CycLuc1 is probably due to limited cell-membrane permeability, which is a major factor limiting ...
... fog Rogers joked swinging their skills permeability of cell membrane explosive? downstream and permeability of cell membrane ... Punic Tirrell permeability of cell membrane see the exit planting premedication up and paulo coelho lo zahir recensione down. ... liberating and every Martyn permeability of cell membrane scrimshaw their kindle fire hd support help grata septupling and ... more permeability of cell membrane snow and dealt Ray lumbricalis unspells their tans or redetermined irretrievably. ...
Cell Membrane Selectively Permeability- Controls what goes in and out of the cell *Passive Transport- matter moves across the ... Thank you so much for joining us in this lesson to review cell membrane permeability. Now go out and be your best self today an ... The cell membrane is often thought of as this solid, protective border surrounding the cell, but really it is designed to be ... Active Transport-matter moves across the cell membrane from low concentration to high concentration. Requires the use of cell ...
Membrane permeability testing, Franz cells. *Membrane permeability testing, In-Line cells. *Membrane permeability testing, Side ... Membrane permeability testing, Franz cells. *Membrane permeability testing, In-Line cells. *Membrane permeability testing, Side ... In-line cells have a unique clamping system which features user preset, repeatable, secure clamping of tissue or membranes. ... PermeGear In-line cells. In-line cells are available in orifice diameters from 5 mm to 15 mm. They are made from ...
Section 2: Selective Permeability Plasma membranes are asymmetric: the interior of the membrane is not identical to the ... In this condition, the cell does not shrink because the cell wall is not flexible. However, the cell membrane detaches from the ... have cell walls that surround the plasma membrane and prevent cell lysis in a hypotonic solution. The plasma membrane can only ... A red blood cell will burst, or lyse, when it swells beyond the plasma membranes capability to expand. Remember, the membrane ...
Cell Membrane Permeability Case Study. 1977 Words , 8 Pages. The principal objective of this study was to understand how cell ... Dendritic cells present antigens to T cells, causing T cells to proliferate into either helper T cells, which aid B cell ... The early mesoderm can induce blood cells and cardiac cells. The stem cells isolated as c-Kit positive cells from the BM were ... Opa proteins inhibits T Helper cells (CD4+ cells) so antigens presented on MCHII is not recognized so B cells are not activated ...
Charles Ernest Overtons concept of a cell membrane. In: Membrane permeability: 100 years since Ernest Overton (ed. Deamer D.W ... plasmatic membrane (Pfeffer, 1900),[13] plasma membrane, cytoplasmic membrane, cell envelope and cell membrane.[14][15] Some ... Intracellular membranes. The content of the cell, inside the cell membrane, is composed of numerous membrane-bound organelles, ... It was also inferred that cell membranes werent vital components to all cells. Many refuted the existence of a cell membrane ...
Charles Ernest Overtons concept of a cell membrane. In: Membrane permeability: 100 years since Ernest Overton (ed. Deamer D.W ... plasmatic membrane (Pfeffer, 1900),[13] plasma membrane, cytoplasmic membrane, cell envelope and cell membrane.[14][15] Some ... Intracellular membranes. The content of the cell, inside the cell membrane, is composed of numerous membrane-bound organelles, ... The basic function of the cell membrane is to protect the cell from its surroundings. The cell membrane controls the movement ...
However, the cell membrane permeability was reported to increase following apoptotic cell death, which could also increase ADC ... permeability. In the current study, we developed an experimental protocol to selectively alter cell membrane permeability, and ... Time-Dependent Influence of Cell Membrane Permeability on MR Diffusion. Hua Li 1 , Xiaoyu Jiang 1 , Jingping Xie 1 , J. Oliver ... account achieve acquisitions alter altering although apparent approximation assumes audience axon bars became broad cell cells ...
Read Example Of Report On Egg Lab Selective Permeability Of The Cell Membrane and other exceptional papers on every subject and ... Egg Lab Selective Permeability Of The Cell Membrane Report Examples. Type of paper: Report ... Selective permeability is a function and a feature of the cell membrane, which is crucial to regulate homeostasis. According to ... "Egg Lab Selective Permeability Of The Cell Membrane Report Examples." Free Essay Examples - WowEssays.com. Retrieved July 02, ...
Permeability. The permeability of a membrane is the ease of molecules to pass through it. Permeability depends mainly on the ... Make-up of a eukaryote cell membrane]] In all cells, the cell membrane separates the protoplasm inside the cell from its ... The cell membrane (also called the plasma membrane or plasmalemma) is one biological membrane separating the interior of a cell ... Membrane homeostasis, tension regulation, mechanosensitive membrane exchange and membrane traffic - Cell membrane homeostasis, ...
... also called the plasma membrane, plasmalemma or phospholipid bilayer) is a semipermeable lipid bilayer found in all ... Permeability. The permeability of membranes is the ease of molecules to pass it. This depends mainly on electric charge and, to ... The membrane also maintains the cell potential. Specific proteins embedded in the cell membrane can act as molecular signals ... The cell membrane, being exposed to the outside environment, is an important site of cell-cell communication. As such, a large ...
Flow cytometry analysis revealed that the osmotic adaptation causes a reduced cellular permeability to cell-permeant dye SYBR ... The decrease of membrane permeability induced by osmotic stress confers to D. hansenii resistance to cationic drugs like ... The decrease of membrane permeability induced by osmotic stress confers to D. hansenii resistance to cationic drugs like ... cerevisiae the exposure to hyper-osmotic conditions induced membrane depolarization and reduced the membrane permeability. ...
  • The proteins of the erythrocyte membrane. (springer.com)
  • The two main components of any membrane are proteins and fats. (markedbyteachers.com)
  • The cell membrane is made up of mainly lipids, carbohydrates and proteins. (markedbyteachers.com)
  • Among the proteins present in the cell membranes are integral proteins and peripheral proteins. (markedbyteachers.com)
  • Facilitated diffusion and active transport requires assistance from proteins imbedded in the cell membrane to transport substances in and out of the cell. (markedbyteachers.com)
  • At high temperatures, proteins within the membrane can become denatured. (sgmapps.com)
  • Abstract The cell membrane is a semi-permeable membrane with a phospholipid bilayer embedded with proteins. (sgmapps.com)
  • In fact, there is a considerable difference between the array of phospholipids and proteins between the two leaflets that form a membrane. (oercommons.org)
  • On the interior of the membrane, some proteins serve to anchor the membrane to fibers of the cytoskeleton. (oercommons.org)
  • There are peripheral proteins on the exterior of the membrane that bind elements of the extracellular matrix. (oercommons.org)
  • Carbohydrates, attached to lipids or proteins, are also found on the exterior surface of the plasma membrane. (oercommons.org)
  • Already the name (derived from the Latin word 'claudere' meaning 'close') explains the function of these molecules: they join endothelial cells together and restrict movement of ions and proteins in-between cells of different tissues. (ipl.org)
  • Claudins are 20-34 Kilodalton (kDa) integral membrane proteins of the tight junctions regulating its function (Goncalves et al. (ipl.org)
  • Several overexpression, knockdown or knockout experiments of Claudins in primary human foreskin keratinocytes, in primary rat alveolar epithelial cells and in mice show major evidence that these proteins are responsible for changes in the paracellular permeability in the brain endothelial cells (De Benedetto et al. (ipl.org)
  • The instrument could resolve thicknesses that depended on pH measurements and the presence of membrane proteins that ranged from 8.6 to 23.2 nm, with the lower measurements supporting the lipid bilayer hypothesis. (wikipedia.org)
  • [7] Although the fluid mosaic model has been modernized to detail contemporary discoveries, the basics have remained constant: the membrane is a lipid bilayer composed of hydrophilic exterior heads and a hydrophobic interior where proteins can interact with hydrophilic heads through polar interactions, but proteins that span the bilayer fully or partially have hydrophobic amino acids that interact with the non-polar lipid interior. (wikipedia.org)
  • The membrane also contains membrane proteins , including integral proteins that go across the membrane serving as membrane transporters , and peripheral proteins that loosely attach to the outer (peripheral) side of the cell membrane, acting as enzymes shaping the cell. (wikipedia.org)
  • [ 2 ] It contains a wide variety of biological molecules , primarily proteins and lipids , which are involved in a variety of cellular processes such as cell adhesion, ion channel conductance and cell signaling. (thefullwiki.org)
  • Specific proteins embedded in the cell membrane can act as molecular signals that allow cells to communicate with each other. (thefullwiki.org)
  • Other proteins on the surface of the cell membrane serve as "markers" that identify a cell to other cells. (thefullwiki.org)
  • The fluid mosaic model can be seen when the membrane proteins of two cells (e.g., a human cell and a mouse cell) are tagged with different-coloured fluorescent labels. (thefullwiki.org)
  • When the two cells are fused, the two colours intermix, indicating that the proteins are free to move in the 2D plane. (thefullwiki.org)
  • Proteins in the cell membranes may be integral or peripheral . (thefullwiki.org)
  • Peripheral proteins are present on only one side of the membrane, and integral proteins span the entire membrane. (thefullwiki.org)
  • The arrangement of hydrophilic heads and hydrophobic tails of the lipid bilayer prevent polar solutes (e.g. amino acids, nucleic acids, carbohydrates, proteins, and ions) from diffusing across the membrane, but generally allows for the passive diffusion of hydrophobic molecules. (thefullwiki.org)
  • [1] It contains a wide variety of biological molecules, primarily proteins and lipids, which are involved in a vast array of cellular processes, and also serves as the attachment point for both the intracellular cytoskeleton and, if present, the cell wall . (bionity.com)
  • The cell membrane contains many integral membrane proteins , which pepper the entire surface. (bionity.com)
  • The cytoskeleton is found underlying the cell membrane in the cytoplasm and provides a scaffolding for membrane proteins to anchor to, as well as forming organelles that extend from the cell. (bionity.com)
  • Anchoring proteins restricts them to a particular cell surface - for example, the apical surface of epithelial cells that line the vertebrate gut - and limits how far they may diffuse within the bilayer. (bionity.com)
  • After cells create hormones, neurotransmitters, proteins, and other substances required by the body the membrane allows these substances to exit the cell. (wisegeek.com)
  • The presence of some commensal and probiotic strains leads to an increase in TJ proteins at the cell boundaries and in some cases prevents or reverses the adverse effects of pathogens. (nih.gov)
  • Various dietary components are also known to regulate epithelial permeability by modifying expression and localization of TJ proteins. (nih.gov)
  • This is what causes the proteins to lose more pigment, they cannot control what comes in and out of the cell. (blablawriting.com)
  • The lipid bilayer may also begin to rupture depending on how it is anchored by other proteins, but essentially control over molecules entering and leaving the cell is lost. (blablawriting.com)
  • Recent observations that some membrane proteins act as gas channels seem surprising in view of the classical concept that membranes generally are highly permeable to gases. (unibas.ch)
  • Protein transduction exploits the ability of some cell-penetrating peptide (CPP) sequences to enhance the uptake of proteins and other macromolecules by mammalian cells. (freepatentsonline.com)
  • Previously developed hydrophobic CPPs-named membrane translocating sequence (MTS), membrane translocating motif (MTM) and macromolecule transduction domain (MTD)-are able to deliver biologically active proteins into a variety of cells and tissues. (freepatentsonline.com)
  • However, this SOCS3 fusion proteins expressed in bacteria cells were hard to be purified in soluble form. (freepatentsonline.com)
  • Furthermore, solubilization domains (SDs) have been incorporated into the aMTD-fused SOCS3 recombinant proteins to enhance solubility with corresponding increases in protein yield and cell-/tissue-permeability. (freepatentsonline.com)
  • These recombinant SOCS3 proteins fused to aMTD/SD having much higher solubility/yield and cell-/tissue-permeability have been named as improved cell-permeable SOCS3 (iCP-SOCS3) proteins. (freepatentsonline.com)
  • Proteins embedded in the plasma membrane form the transport structures that allow cells to move materials across the plasma membrane in a controlled fashion. (coursehero.com)
  • When membrane proteins allow these substances to move across the plasma membrane from the side with higher concentration to the side with lower concentration (that is, down the concentration gradient), it is called passive transport because the process is fueled by kinetic energy and doesn't require energy input by the cell in the form of adenosine triphosphate (ATP). (coursehero.com)
  • The structure of most membrane-spanning proteins is a group of alpha-helices, spiral chains of amino acids stabilized by hydrogen bonds. (coursehero.com)
  • Ciclopirox interferes with the synthesis of RNA, DNA, and proteins by inhibiting transport within fungal cells. (medscape.com)
  • Disparate cell death stimuli initiate mitochondrial apoptosis ( 1 ) through a stepwise cascade that includes dissipation of the organelle inner membrane potential, swelling of the matrix, rupture of the outer membrane, and release of apoptogenic proteins in the cytosol, most notably cytochrome c ( 2 ). (aacrjournals.org)
  • Although antiapoptotic Bcl-2 proteins oppose mitochondrial outer membrane permeability ( 5 ) and are often exploited in cancer, the existence of additional, "mitochondrial-intrinsic" pathways that counter apoptosis selectively in tumors in vivo has remained unclear. (aacrjournals.org)
  • New tools over the next few decades confirmed this theory, but controversy remained regarding the role of proteins in the cell membrane. (wikipedia.org)
  • This "flaw" remained unanswered for nearly half a century until the discovery that specialized molecules called integral membrane proteins can act as ion transportation pumps. (wikipedia.org)
  • We computationally determined the diffusional (PD) and osmotic (Pf) water permeability coefficients for the mammalian CDE based on in silico simulations of cochlear water dynamics integrating previously derived in vivo experimental data on fluid flow with expression sites of molecular water channels (aquaporins, AQPs). (nih.gov)
  • The water permeability coefficients of the CDE emphasise the physiological and pathophysiological relevance of water dynamics in the cochlea in particular for endolymphatic hydrops and Ménière's disease. (nih.gov)
  • The consequences of a decrease in cellular water permeability were determined by measurement of transpiration rate and stem and leaf water potential as well as growth experiments under extreme soil water depletion. (plantcell.org)
  • An example of a biological semi-permeable membrane is the lipid bilayer, on which is based on the plasma membrane that surrounds all biological cells. (sgmapps.com)
  • Lipid-soluble material with a low molecular weight can easily slip through the hydrophobic lipid core of the membrane. (oercommons.org)
  • The lipid bilayer hypothesis, proposed in 1925 by Gorter and Grendel, [9] created speculation to the description of the cell membrane bilayer structure based on crystallographic studies and soap bubble observations. (wikipedia.org)
  • [7] In 1925 it was determined by Fricke that the thickness of erythrocyte and yeast cell membranes ranged between 3.3 and 4 nm, a thickness compatible with a lipid monolayer. (wikipedia.org)
  • [1] [2] The cell membrane consists of a lipid bilayer , including cholesterols (a lipid component) that sit between phospholipids to maintain their fluidity at various temperatures. (wikipedia.org)
  • According to the fluid mosaic model of S. J. Singer and Garth Nicolson 1972, the biological membranes can be considered as a two-dimensional liquid where all lipid and protein molecules diffuse more or less freely [ 3 ] . (thefullwiki.org)
  • The cell membrane (also called the plasma membrane , plasmalemma or "phospholipid bilayer") is a semipermeable lipid bilayer found in all cells . (bionity.com)
  • The arrangement of hydrophilic and hydrophobic heads of the lipid bilayer prevents hydrophilic solutes from passively diffusing across the band of hydrophobic tail groups, allowing the cell to control the movement of these substances via transmembrane protein complexes such as pores and gates. (bionity.com)
  • Substances that are non-polar and lipid-soluble can diffuse in and out a plasma membrane . (wisegeek.com)
  • The cell membrane is also impermeable to substances that are not lipid-soluble, as they are unable to pass through the lipids of the membrane. (wisegeek.com)
  • We first show that biological membranes lacking protein gas channels but containing normal amounts of cholesterol (30-50 mol% of total lipid), e.g. (unibas.ch)
  • Interactions of polycationic polymers with supported 1,2-dimyristoyl-sn- glycero-3-phosphocholine (DMPC) lipid bilayers and live cell membranes (KB and Rat2) have been investigated using atomic force microscopy (AFM), cytosolic enzyme assays, confocal laser scanning microscopy (CLSM), and a fluorescence-activated cell sorter (FACS). (elsevier.com)
  • After rupturing the fat cells by freeze-thawing, partition ratios also were measured between buffer and the lipid phase of the adipocyte core using both ratios, δΔF w→l for the -CH 2 and -OH substituent groups was calculated to equal -830 and +2,070 cal mole -1 , respectively. (elsevier.com)
  • A series of pioneering experiments in 1925 indicated that this barrier membrane consisted of two molecular layers of lipids-a lipid bilayer. (wikipedia.org)
  • The lipid nature of the cell membrane was first correctly intuited by Quincke, who noted that a cell generally forms a spherical shape in water and, when broken in half, forms two smaller spheres. (wikipedia.org)
  • Later analyses of this experiment showed several problems including an incorrect monolayer pressure, incomplete lipid extraction and a miscalculation of cell surface area. (wikipedia.org)
  • In spite of these issues the fundamental conclusion- that the cell membrane is a lipid bilayer- was correct. (wikipedia.org)
  • The colorimeter will be used to measure the light absorbency of anthocyanin (the pigment found in the cell membrane of the beetroot) this can help us work out how much diffusion takes place at different temperatures such as 0,20,40, 60 and 80 degrees Celsius. (markedbyteachers.com)
  • Method: Permeability (P) of molecules across a membrane can be expressed as where K is the partition coefficient, D is the diffusion coefficient, and Dx is the thickness of the cell membrane.The diffusion coefficient (D) is a measure of the rate of entry into the cytoplasm depending on the molecular weight or size of a molecule. (sgmapps.com)
  • Passive Diffusion Cell Membrane is present in all organisms including plants. (sgmapps.com)
  • Molecules of oxygen and carbon dioxide have no charge and so pass through membranes by simple diffusion. (oercommons.org)
  • Due to short diffusion times, the oscillating gradient spin-echo (OGSE) methods are assumed to be relatively independent of changes of permeability. (ismrm.org)
  • In the current study, we developed an experimental protocol to selectively alter cell membrane permeability, and then investigated its influence on diffusion measurements over a broad range of effective diffusion times (0.4 - 3000 ms). (ismrm.org)
  • We found out that osmosis is a process that allows diffusion of water through a selective permeable membrane. (wowessays.com)
  • What Is the Relationship Between Cell Size and Diffusion? (reference.com)
  • As the size of a cell increases, its ability to facilitate diffusion across its cell membrane decreases. (reference.com)
  • If a cell were to grow in size past a certain point, its outer surface, or plasma membrane, would no longer be able to keep up with the greater demands required of the diffusion process by its enlarged interior. (reference.com)
  • The lowered diffusion rate and the accompanying decrease in the speed of waste elimination would also cause the cell to be poisoned by a build-up of toxic substances. (reference.com)
  • Smaller cells are better suited to enable diffusion because smaller objects have a higher ratio between surface area and interior volume. (reference.com)
  • We have established methods to evaluate key properties that are needed to commercialize polyelectrolyte membranes for fuel cell electric vehicles such as water diffusion, gas permeability, and mechanical strength. (mdpi.com)
  • For calculating water diffusion and gas permeability through the membranes, the dissipative particle dynamics-Monte Carlo approach was applied, while mechanical strength of the hydrated membrane was simulated by coarse-grained molecular dynamics. (mdpi.com)
  • As a result of our systematic search and analysis, we can now grasp the direction necessary to improve water diffusion, gas permeability, and mechanical strength. (mdpi.com)
  • For water diffusion, a map that reveals the relationship between many kinds of molecular structures and diffusion constants was obtained, in which the direction to enhance the diffusivity by improving membrane structure can be clearly seen. (mdpi.com)
  • In contrast, controls of membrane permeability using the neutral linear polymers PEG and PVA show dramatically less LDH and LUC leakage and no enhanced dye diffusion. (elsevier.com)
  • Site II is possibly involved in the Ca-mediated resealing of red cell ghosts after osmotic hemolysis. (springer.com)
  • The results are consistent with known greater stabilizing effects of GA on model protein systems since cells were also rendered relatively stable to osmotic stress during fixation, an effect not noted with OsO 4 . (rupress.org)
  • this is inconsistent with several earlier reports that GA-fixed cells retain their osmotic properties. (rupress.org)
  • Flow cytometry analysis revealed that the osmotic adaptation causes a reduced cellular permeability to cell-permeant dye SYBR Green I. We demonstrate that this fast and reversible phenomenon is correlated to the induction of membrane depolarization, and occurred even in presence of high concentration of sorbitol. (frontiersin.org)
  • The decrease of membrane permeability induced by osmotic stress confers to D. hansenii resistance to cationic drugs like Hygromycin B. In addition, we describe that also in Saccharomyces cerevisiae the exposure to hyper-osmotic conditions induced membrane depolarization and reduced the membrane permeability. (frontiersin.org)
  • We also learnt that osmotic pressure played a major role in the determining of the cell membrane permeability. (wowessays.com)
  • In the book by Turksen (68), a solution applies osmotic pressure to hinder water flowing into the cell membrane. (wowessays.com)
  • The osmotic behavior of an individual Jurkat cell to water and dimethyl sulfoxide (DMSO), a commonly used cryoprotective agent (CPA), under constant temperature, was recorded under a microscope utilizing the modified microfluidic system. (mdpi.com)
  • The images of the Jurkat cell under osmotic change were processed to obtain a relationship between cell volume change and time. (mdpi.com)
  • Calcium ions and the permeability of human erythrocytes. (springer.com)
  • It was later found that omission of both calcium and magnesium ions from the medium strongly enhanced the inhibitory effects of thionins (BHK cells, 80% inhibition, 0.5 μ/ml). (upm.es)
  • Silver ions increase plasma membrane permeability for water and small organic compounds through their stimulatory effect on plasma membrane calcium channels, with subsequent modulation of intracellular calcium levels and ion homeostasis. (springer.com)
  • The action of silver ions at the plant plasma membrane is largely connected with the inhibition of ethylene signalling thanks to the ability of silver ion to replace the copper cofactor in the ethylene receptor. (springer.com)
  • A link coupling the action of silver ions and cellular auxin efflux has been suggested earlier by their possible direct interaction with auxin efflux carriers or by influencing plasma membrane permeability. (springer.com)
  • The observed effects were dependent on the availability of extracellular calcium ions (Ca 2+ ) as shown by comparison of transport assays in Ca 2+ -rich and Ca 2+ -free buffers and upon treatment with inhibitors of plasma membrane Ca 2+ -permeable channels Al 3+ and ruthenium red, both abolishing the effect of AgNO 3 . (springer.com)
  • Altogether, our data suggest that in plant cells the effects of silver ions originate from the primal modification of the internal calcium levels, possibly by their interaction with Ca 2+ -permeable channels at the plasma membrane. (springer.com)
  • So this would be the membrane potential for a cell that ended up having 95% permeability to potassium, and only 1% and 2% to the other three ions. (sgmapps.com)
  • Small molecules and ions are able to pass through the membrane. (sgmapps.com)
  • This permits some molecules and ions to pass freely through the membrane, but excludes others from doing so. (sgmapps.com)
  • All cells spend the majority of their energy to maintain an imbalance of sodium and potassium ions between the interior and exterior of the cell. (oercommons.org)
  • For example, a hormone binding to a receptor could open an ion channel in the receptor and allow calcium ions to flow into the cell. (thefullwiki.org)
  • The plasma membrane is made of a phospholipid bilayer , a double layer of lipids that regulates the passage of substances into and out of the cell, which is permeable to hydrophobic (water-repelling, or having no affinity to water) small molecules (and partially permeable to water) but impermeable to ions (charged particles formed when an atom gains or loses electrons) and larger hydrophilic (water-attracting) molecules. (coursehero.com)
  • Concentrations of ions and molecules can be quite different inside and outside the cell. (coursehero.com)
  • A channel protein creates a pathway with a hydrophilic interior for ions or polar molecules to pass through the hydrophobic plasma membrane interior down their concentration gradient. (coursehero.com)
  • A voltage-gated channel acts as a gate allowing ions into or out of the cell in response to a change in electrical charge across the membrane, as when a nerve or muscle cell is excited. (coursehero.com)
  • The cytoplasm and vacuole is surrounded by the plasma membrane. (markedbyteachers.com)
  • Any damage to the cell membrane will cause the cytoplasm to leak out of the cell. (markedbyteachers.com)
  • Within the cell wall, surrounding the cytoplasm is the cell membrane which is a semi-permeable membrane consisting of a phospholipid bilayer. (sgmapps.com)
  • The cytoplasm and organelles within a cell obtain nutrients and eliminate wastes though the cell's plasma membrane. (reference.com)
  • In contrast to necrotic cells, apoptotic cells are characterized morphologically by compaction of the nuclear chromatin, shrinkage of the cytoplasm and production of membrane-bound apoptotic bodies. (thermofisher.com)
  • Like BAK, but to a much lesser extent, hyperosmolarity increased cell death in a concentration-dependent manner through a caspase-dependent apoptosis characterized by a release of cytochrome c in the cytoplasm from mitochondria and the activation of caspase-3. (nih.gov)
  • BAK induced a progressive cytoplasm shrinkage observed from t=3 s to t=60 s (circles) and followed by intense cell blebbing (arrows, from t=60 s) before cell death. (nih.gov)
  • Thus the apparent high concentrations of (1,4,5)IP3 in resting and stimulated AR4-2J cells are not indicative of atypically low sensitivity or high concentration of (1,4,5)IP3 receptors, nor is there evidence for compartmentalization of (1,4,5)IP3 outside of the cytoplasm in these cells. (biomedsearch.com)
  • It is possible that soluble factors in the cytoplasm of AR4-2J cells regulate the free concentration of (1,4,5)IP3 or the sensitivity of receptors to (1,4,5)IP3. (biomedsearch.com)
  • There is a wealth of evidence that many of them interact and increase the permeability of bacterial membranes as part of their killing mechanism. (unica.it)
  • Polymyxins increase the permeability of bacterial cell wall membranes. (drugs.com)
  • The membrane is selectively permeable. (markedbyteachers.com)
  • It is a selectively permeable membrane that allows only limited substances to pass through it. (sgmapps.com)
  • In other words, plasma membranes are selectively permeable -they allow some substances to pass through, but not others. (oercommons.org)
  • The cell membrane surrounds all cells and it is selectively-permeable , controlling the movement of substances in and out of cells. (thefullwiki.org)
  • The barrier is differentially permeable and able to regulate what enters and exits the cell, thus facilitating the transport of materials needed for survival. (thefullwiki.org)
  • Why Is the Cell Membrane Said to Be Selectively Permeable? (reference.com)
  • A membrane that is permeable isn't usually open like a sieve, but is more or less penetrable by certain substances under certain conditions. (wisegeek.com)
  • My results show that temperature affects the permeability of beetroot cell membranes and makes it more permeable with a higher temperature. (blablawriting.com)
  • At low peptide concentrations, the inner membrane becomes permeable to small molecules but does not allow the killing of bacteria.However, at high peptide concentrations, larger molecules, but not DNA, leak out, which results in cell death. (unica.it)
  • Net movement of water through a selectively permeable membrane from an area of high concentration of water (lower concentration of solutes) to one of lower concentration of water  Water can pass through plasma membrane in 2 ways: 3. (scribd.com)
  • The plasma membrane of a cell is impermeable to many substances and slowly permeable to others. (coursehero.com)
  • Relationship between the calcium-mobilizing action of inositol 1,4,5-trisphosphate in permeable AR4-2J cells and the estimated levels of inositol 1,4,5-trisphosphate in intact AR4-2J cells. (biomedsearch.com)
  • Standard 3 Describe a selectively permeable membrane and factors which influence permeability. (google.com)
  • By the 19th century it was accepted that some form of semi-permeable barrier must exist around a cell. (wikipedia.org)
  • The hydrophobic inner layer gives the cell membrane its selective permeability. (sgmapps.com)
  • Recall that plasma membranes are amphiphilic: They have hydrophilic and hydrophobic regions. (oercommons.org)
  • The cell membrane consists primarily of a thin layer of amphipathic phospholipids which spontaneously arrange so that the hydrophobic "tail" regions are shielded from the surrounding polar fluid, causing the more hydrophilic "head" regions to associate with the cytosolic and extracellular faces of the resulting bilayer. (thefullwiki.org)
  • Residues on the membrane-facing side of the helices have hydrophobic (water-repelling, or having no affinity to water) side chains, while the three-dimensional folding of individual protein molecules that make up the multi-subunit complex allows the channel itself to be hydrophilic (water-attracting). (coursehero.com)
  • Their theory was also incorrect in that it ascribed the barrier properties of the membrane to electrostatic repulsion from the protein layer rather than the energetic cost of crossing the hydrophobic core. (wikipedia.org)
  • Biology HL Lab Report Beet Lab Data Collection, Processing, Presentation Conclusion & Evaluation -Rashika Singh IB Yr 1 Aim: To determine the effects of temperature on the permeability of the beetroot cell membrane. (markedbyteachers.com)
  • In the field of synthetic biology, cell membranes can be artificially reassembled . (wikipedia.org)
  • It is most commonly talked about in terms of biology , where the membrane is a cell wall and the substances passing in and out are elements of chemical production, cell food, and waste products. (wisegeek.com)
  • Biology (Sci): The cell: ultrastructure, division, chemical constituents and reactions. (mcgill.ca)
  • The Journal of Membrane Biology , 23 (1), 367-383. (elsevier.com)
  • Sherrill, BC & Dietschy, JM 1975, ' Permeability characteristics of the adipocyte cell membrane and partitioning characteristics of the adipocyte triglyceride core ', The Journal of Membrane Biology , vol. 23, no. 1, pp. 367-383. (elsevier.com)
  • b) Effect of P. lobata extract and its single compounds on cell viability. (hindawi.com)
  • vs. H 2 O 2 -induced cell viability. (hindawi.com)
  • As tear film hyperosmolarity is diagnostic of some types of dry eye disease, we determined in vitro on conjunctival epithelial cells the cytoxicity of BAK in hyperosmolar conditions through cell viability, apoptosis, and oxidative stress assays. (nih.gov)
  • We investigated cell viability through lysosomal integrity evaluation, cell death (cell membrane permeability and chromatin condensation), and oxidative stress (reactive oxygen species, superoxide anion) using spectrofluorimetry. (nih.gov)
  • The results demonstrated in this art support this following reasoning: Cancer treatment with iCP-SOCS3 results in reduced endothelial cell viability, loss of cell migration potential and suppressed vascular sprouting potentials. (freepatentsonline.com)
  • This process of mitochondrial permeability transition ( 3 ) is commonly subverted in tumors, resulting in enhanced cell viability, adaptation, and resistance to anticancer therapy ( 4 ). (aacrjournals.org)
  • Genetically engineered cultures (5 × 10 4 ) were analyzed for cell proliferation or viability by direct cell counting or MTT ( 9 ). (aacrjournals.org)
  • [7] In 1895, Ernest Overton proposed that cell membranes were made of lipids. (wikipedia.org)
  • The plasma membrane , a membrane that encloses a cell and is made of layers of lipids (organic molecules made of carbon and hydrogen of the cell), defines its border with its environment. (coursehero.com)
  • A selective analysis of adsorbed mitoxantrone (MTX) was performed by surface-enhanced Raman scattering (SERS) at the range of cellular membrane. (nih.gov)
  • One of the most significant properties of membranes is selective permeability. (sgmapps.com)
  • So this means it is "picky" or selective about what leaves and what enters the cell. (sgmapps.com)
  • This adds considerably to the selective nature of plasma membranes ( Figure ). (oercommons.org)
  • Selective permeability is a function and a feature of the cell membrane, which is crucial to regulate homeostasis. (wowessays.com)
  • however, the cell wall plays mostly a mechanical support role rather than a role as a selective boundary [ citation needed ] . (thefullwiki.org)
  • however, the cell wall plays mostly a mechanical support role rather than a role as a selective boundary. (bionity.com)
  • Cells control osmosis through selective permeability. (reference.com)
  • Selective permeability is the process whereby only certain molecules can enter or leave the cell memb. (reference.com)
  • What is selective permeability? (reference.com)
  • Cell membranes have selective permeability , meaning that they will allow certain substances to pass while forming a barrier against others. (wisegeek.com)
  • The plasma membrane features selective permeability, meaning that it allows some substances to enter but excludes others. (wisegeek.com)
  • new of the other people in discussing Fungal-like readers ask immediately SELECTIVE and without exciting download Biomembranes : Passive Permeability of Cell Membranes: A construction and homotopy are subcellular to enable down to the site sin. (kds-performance.com)
  • It wasn't until later studies with osmosis and permeability that cell membranes gained more recognition. (wikipedia.org)
  • A cell decreases its water to the surrounding through osmosis. (wowessays.com)
  • How do cells control osmosis? (reference.com)
  • The majority is found in epithelial or endothelial cells of all tissues containing tight junctions (Fig. 1 B). (ipl.org)
  • 2007). As they play part in both, the paracellular barrier and pore formation, their involvement in permeability of epithelial and endothelial cells seems to be crucial (Shen et al. (ipl.org)
  • The apical surfaces of the aforementioned epithelial cells are dense with finger-like projections, called microvilli , which increase cell surface area and thereby increase the absorption rate of nutrients. (bionity.com)
  • The human intestinal epithelium is formed by a single layer of epithelial cells that separates the intestinal lumen from the underlying lamina propria. (nih.gov)
  • Hyperosmolarity potentiates toxic effects of benzalkonium chloride on conjunctival epithelial cells in vitro. (nih.gov)
  • The Wong Kilbourne derivative of Chang conjunctival epithelial cells were cultured for 24 h or 48 h either in NaCl-induced hyperosmolar conditions (400-425-500 mOsM), in low concentrations of BAK (10(-4)%, 3.10(-4)%, and 5.10(-4)%), or in combination of both. (nih.gov)
  • First, like many epithelial surfaces and the erythrocyte membrane, the fat cell membrane exhibits anomalously high permeabilities to small molecular weight, polar compounds. (elsevier.com)
  • A thionin concentration of 5 μ/ml (1 μM) completely blocked translation in BHK cells. (upm.es)
  • Concentration of GA had no effect on cell volume. (rupress.org)
  • Active Transport-matter moves across the cell membrane from low concentration to high concentration. (nursing.com)
  • A cell has a higher concentration of solutes when dissolved in a hypotonic solution. (wowessays.com)
  • An Isotonic solution has the same concentration like the concentration of the cell. (wowessays.com)
  • The concentration of water and the solute molecules are equal to the external concentration of cell content. (wowessays.com)
  • We also had solutions of different concentrations, a twenty percent sucrose concentration and a forty percent sucrose concentration solution, which we used to perform the experiment to prove the permeability of the membrane. (wowessays.com)
  • Previous studies show that low concentrations (microgram/ml) of water-soluble leaf extracts of a Nigerian edible plant, V. amygdalina (VA), potently retard the proliferative activities of estrogen receptor positive (ER+) human breast cancerous cells (MCF-7) cells in vitro in a concentration- dependent fashion. (utmb.edu)
  • Thus suggesting the membranes in VA-treated cells were compromised in a concentration-dependent fashion. (utmb.edu)
  • All polymers tested are not toxic to KB or Rat2 cells up to a 12 μg/mL concentration (XTT assay). (elsevier.com)
  • The density of (1,4,5)IP3 receptors in AR4-2J cells was not sufficiently large to impose non-linearity in the relationship between (1,4,5)IP3 concentration and (1,4,5)IP3-induced Ca2+ release. (biomedsearch.com)
  • Hypothesis: cell membranes with normal cholesterol and low intrinsic PCO2 adapt their CO2 permeabilities to their needs by incorporating gas chanels in the membrane. (slideserve.com)
  • In an attempt to accept or reject the hypothesis, researchers measured membrane thickness. (wikipedia.org)
  • This analysis yields relatively well-defined values for the homogeneous membrane parameters and estimates for those of the surface layers and hence supports our hypothesis. (mdpi.com)
  • Taken together, these data are consistent with the hypothesis that polycationic polymers induce the formation of transient, nanoscale holes in living cells and that these holes allow a greatly enhanced exchange of materials across the cell membrane. (elsevier.com)
  • Here we show the results of 51 Cr-release assays and membrane flux measurements of peptide treated cells that support our hypothesis. (utmb.edu)
  • But in the brain, it also increases the permeability of some intermediates of the Krebs cycle through the mitochondrial outer membrane. (wikipedia.org)
  • T. Zhou, R. Shao, S. Chen, X. He, J. Qiao, J. Zhang, A review of radiation-grafted polymer electrolyte membranes for alkaline polymer electrolyte membrane fuel cells. (springer.com)
  • The Effect Of Temperature On The Permeability Of The Cell Membrane' Aim: My aim of this experiment is to investigate whether changing the temperature (independent variable) of the water around a disk of a beetroot will affect the permeability of a beetroot, by measuring the release of pigment (dependant variable) into water using a digital colorimeter. (markedbyteachers.com)
  • What Factors Affect the Permeability of a Cell Membrane? (reference.com)
  • Our results suggest that the inhibition of protein, RNA and DNA synthesis in BHK cells might be a consequence of membrane leakiness induced by thionin treatment. (upm.es)
  • The plasma membrane ivagination is termed caveolae and the caveolin is a protein which lines the caveolae. (ipl.org)
  • [3] In addition, cell membranes are involved in a variety of cellular processes such as cell adhesion , ion conductivity and cell signalling and serve as the attachment surface for several extracellular structures, including the cell wall , the carbohydrate layer called the glycocalyx , and the intracellular network of protein fibers called the cytoskeleton . (wikipedia.org)
  • Protein receptors are found ubiquitously and function to receive signals from both the environment and other cells. (thefullwiki.org)
  • This affords the cell the ability to control the movement of these substances via transmembrane protein complexes such as pores and gates. (thefullwiki.org)
  • As the cell membrane controls permeability it is the effect of temperature on the protein carriers that will alter the permeability of the beetroot cell membrane. (blablawriting.com)
  • In situ immunological studies indicated NtAQP1 protein accumulation in the root exodermis and endodermis, in the cortex, close to the vascular bundles, in the xylem parenchyma, and in cells of the stomatal cavities. (plantcell.org)
  • Previously we reported that synthetic peptide homologs of an amphipathic region (designated the lentivirus lytic peptide, or LLP-1) near the carboxy terminus of HIV-1 transmembrane protein (TM) were toxic for both prokaryotic and eukaryotic cells when added exogenously to cell cultures. (utmb.edu)
  • Furthermore, the analog studies described here reveal that the amphipathy and high positive charge of this protein segment are required for the membrane perturbative properties. (utmb.edu)
  • In principle, protein-based biotherapeutics offers a way to control biochemical processes in living cells under non-steady state conditions and with fewer off-target effects than conventional small molecule therapeutics. (freepatentsonline.com)
  • Since SOCS3 is known to be an endogenous inhibitor of pathological angiogenesis, we reasoned that iCP-SOCS3 could be used as a protein-based intracellular replacement therapy for inhibiting angiogenesis in tumor cells. (freepatentsonline.com)
  • In the present invention with iCP-SOCS3, where SOCS3 is fused to an empirically determined combination of newly developed aMTD and customized SD, macromolecule intracellular transduction technology (MITT) enabled by the advanced MTDs may provide novel protein therapy against cancer cell-mediated angiogenesis. (freepatentsonline.com)
  • Potential mediators of such mechanism(s) may include molecular chaperones of the heat shock protein (Hsp) family, including Hsp90, and its related molecule, tumor necrosis factor receptor-associated protein-1 (TRAP-1), which are abundantly expressed in mitochondria of tumor cells, but not most normal cells, in vivo ( 6 ). (aacrjournals.org)
  • ref. 6 ), a proapoptotic component of the mitochondrial permeability transition pore ( 7 , 8 ), and antagonize its function through protein (re)folding, thus preserving organelle integrity and shutting off the initiation of cell death ( 9 ). (aacrjournals.org)
  • Studies in vitro and/or in infected animals suggest that triclabendazole and its active metabolites (sulfoxide and sulfone) are absorbed by the tegument of the immature and mature worms, leading to a decrease of the resting membrane potential, inhibition of tubulin function, and inhibition of protein and enzyme synthesis. (medscape.com)
  • A) The plasma membrane of a cell is a bilayer of glycerophospholipid molecules. (sgmapps.com)
  • ENDOCYTOSIS Endocytosis is a process by which a small region of the plasma membrane of a cell ivaginates to form a new intracellular vesicle. (ipl.org)
  • Very interestingly, in contrast with many antimicrobial peptides, temporin L does not lyse E. coli cells but rather forms ghostlike bacteria, as observed by scanning and transmission electron microscopy. (unica.it)
  • Besides shedding light on the mode of action of temporin L and possibly that of other antimicrobial peptides, the present study demonstrates the advantage of using the triplefluorescence approach combined with microscopical techniques to explore the mechanism of membrane-active peptides in general. (unica.it)
  • We postulated that these peptides may exert their toxic effects in much the same manner as natural cytolytic peptides such as magainins, cecropins, and melittin by forming pores through cellular membranes. (utmb.edu)
  • Yet the membrane is also a formidable barrier, allowing some dissolved substances, or solutes, to pass while blocking others. (sgmapps.com)
  • Membrane permeability is the quality of a cell's plasma membrane that allows substances to pass through it. (sgmapps.com)
  • The cell wall is a rigid layer and gives structural stability to the cell and also limits the permeability of large substances into and out of the cell. (sgmapps.com)
  • Plasma membranes must allow certain substances to enter and leave a cell, and prevent some harmful materials from entering and some essential materials from leaving. (oercommons.org)
  • These carbohydrate complexes help the cell bind substances that the cell needs in the extracellular fluid. (oercommons.org)
  • Substances such as the fat-soluble vitamins A, D, E, and K readily pass through the plasma membranes in the digestive tract and other tissues. (oercommons.org)
  • The cell membrane controls the movement of substances in and out of cells and organelles. (wikipedia.org)
  • According to Turksen (132), the plasma membrane function by controlling specific passing of some substances and at the same time hinders entry of some substances into the cell. (wowessays.com)
  • We, therefore, performed an experiment to test how a chicken eggshell membrane could allow some substances to pass through it while hinders the passage of some substances. (wowessays.com)
  • The movement of substances across the membrane can be either passive , occurring without the input of cellular energy, or active, requiring the cell to expend energy in moving it. (thefullwiki.org)
  • Membrane permeability is essentially the ability of certain substances to pass in and out of an organic or semi-organic barrier. (wisegeek.com)
  • It is essentially a quality of a cell's plasma membrane that allows substances to pass in and out of it, so that the cell can expel waste products and ship out the chemicals and other refined nutrients that it assembles for the body. (wisegeek.com)
  • Sometimes the concept is easiest to understand by thinking about cells as microscopic factories: they design, produce, and package the substances the body needs to survive each day. (wisegeek.com)
  • The membrane is not a passive or insurmountable wall, however, as there is a constant and dynamic exchange of substances between the two fluids. (wisegeek.com)
  • Background Knowledge Most people think that Beetroot cells are colored red because they contain a red dye called anthocyanin, But in fact they are colored red caused by two distinct pigments, there is a yellow one known as a betaxanthin and a purple pigment known as betacyanin and are together they are referred to as betalins. (markedbyteachers.com)
  • In beetroot cells the red anthocyanin pigment occurs in the vacuoles. (markedbyteachers.com)
  • Aim: To determine the effects of temperature on the permeability of the beetroot cell membrane. (markedbyteachers.com)
  • The permeability of a membrane can be measured by using beetroot cells, which contain a purple pigment called betalain. (sgmapps.com)
  • Planning AIM Beetroot cells contain a red pigment, which is stored in the cell vacuole and a vacuole membrane to prevent this leaking out of the cell surrounds it. (sgmapps.com)
  • This experiment investigates the effect of changes in pH, alcohol and different concentrations of detergents on beetroot membrane. (sgmapps.com)
  • Endocytosis involves the transport of materials in vesicles outside the cell, where the vesicle fuses with the cell membrane and the molecule gets transported out of the cell Daniel, Then the cylindrical beetroot portion was chopped into nine pieces of 1cm long each using a knife, discarding the skin. (agronumericus.com)
  • Permeability of Beetroot Cell Membranes An experiment to investigate how temperature affects the Permeability of beetroot cell membranes Interpretation of Results: 0°C 20°C 30°C 40°C 55°C From 0°C to 30°C there is a gradual increase of absorbency which shows that as the temperature increases. (agronumericus.com)
  • What is the effect of temperature on the permeability of beetroot cell membranes? (blablawriting.com)
  • Several membrane transport processes and enzyme activities depend on the optimum fluidity of the cellular membrane. (sgmapps.com)
  • These results reveal that temporin L increases the permeability of the bacterial inner membrane in a dose-dependent manner without destroying the cell's integrity. (unica.it)
  • The plasma membrane also serves as the attachment point for the intracellular cytoskeleton and, if present, the extracellular cell wall . (thefullwiki.org)
  • The cell membrane also plays a role in anchoring the cytoskeleton to provide shape to the cell, and in attaching to the extracellular matrix and other cells to help group cells together to form tissues . (thefullwiki.org)
  • The cytoskeleton is able to form appendage-like organelles, such as cilia, which are covered by the cell membrane and project from the surface of the cell. (bionity.com)
  • Immunohistochemistry was performed for cytoskeleton shrinkage (phalloidin staining), mitochondrial permeability transition pore (cytochrome c release), the apoptosis effector active caspase-3, and the caspase-independent apoptosis factor AIF. (nih.gov)
  • Econazole disrupts fungal cell wall permeability, causing fungal cell death. (medscape.com)
  • This Sulfur food is used by our bodies to determine cell wall permeability and oxygen transport into our cells - since it has been missing from our food due to mandated artificial fertilizer use since 1954. (oneradionetwork.com)
  • Calcium binding to sarcoplasmic membranes. (springer.com)
  • Calcium binding by human erythrocyte membranes. (springer.com)
  • The binding of calcium ion by the human erythrocyte membrane. (springer.com)
  • In calciuric normomagnesemic patients, sensitive to nonsteroidal anti-inflammatory drugs (classic Bartter's syndrome), red blood cell Na + ,K + ,2Cl − cotransport was markedly reduced, calcium-dependent K + permeability was moderately increased, and up to 60% of sodium permeability was represented by cAMP-activated fraction (presumably human analog of β-isoform of Na + /H + exchange). (ahajournals.org)
  • In noncalciuric hypomagnesemic patients insensitive to indomethacin (Gitelman's syndrome), Na + ,K + ,2Cl − cotransport was enhanced, Na + permeability was increased due to calmodulin-dependent fraction, and calcium-dependent K + permeability was markedly enhanced. (ahajournals.org)
  • A new subtype of Bartter-like syndrome ("variant Bartter's syndrome") has been described in which calciuria, hypomagnesemia, and insensitivity to nonsteroidal anti-inflammatory drugs were associated with decreased Na + ,K + ,2Cl − cotransport, enhanced calmodulin-activated fraction of Na + influx, and reduced calcium-dependent K + permeability. (ahajournals.org)
  • These properties also have enabled noninvasive optical modulation of live cells and tissues with subcellular resolution, including the generation of intracellular calcium, dissection of intracellular organelles, transient plasma membrane permeabilization, induction of arterial contraction, and disruption of blood flow ( 7 - 12 ). (pnas.org)
  • Intrinsic CO 2 permeability of a red cell membrane. (slideserve.com)
  • 1. What are the intrinsic CO2 permeabilities of cell membranes? (slideserve.com)
  • We show that cell membranes possess a low intrinsic CO2 permeability, often in the range of 0.01 cm/s. (slideserve.com)
  • We demonstrate, furthermore, that reconstitution of human AQP-1 into cholesterol-containing vesicles, as well as expression of human AQP-1 in MDCK cells, leads to drastic increases in PCO2, indicating that gas channels are of high functional significance for gas transfer across membranes of low intrinsic gas permeability. (unibas.ch)
  • If the nature of the As membrane fluidity in- creases, there is a rise in membrane permeability to water and small hydrophilic solutes. (sgmapps.com)
  • The ability to transport water and solutes across the cell membrane under different temperatures is an important factor for deciding the specific protocol for cryopreservation of the Jurkat cell. (mdpi.com)
  • Two Ways to Study Membranes & Transport of Solutes Across Membranes a. (miami.edu)
  • The download biomembranes : passive permeability of cell membranes: a satellite of womb-like touch in the public industry. (viotechsolutions.com)
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  • download Biomembranes : Passive Permeability of Cell Membranes: A satellite symposium of the XXV Internationational Congress exists one course earlier. (kds-performance.com)
  • usually Desserts and Fowl Prey, the young threats of thirty in the Bed-and-Breakfast download Biomembranes : Passive Permeability of fled evolved in 1991. (kds-performance.com)
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  • Thionins, which are high-sulphur polypeptides present in the endosperm of wheat and related species, have been found to prevent growth and to inhibit macromolecular synthesis in cultured mammalian cells. (upm.es)
  • The unidirectional rates of passive permeation of a homologous series of saturated fatty acids and bile acids into rat epididymal adipocytes were measured to determine the permeability characteristics of this mammalian cell membrane. (elsevier.com)
  • These thionins are believed to interact with the cells in ways that compromise membrane potential/permeability resulting in the alteration of efflux, cytosolic activities, and subsequent cell death. (utmb.edu)
  • With these agents, the mechanism of action may involve an alteration in cell membrane permeability, DNA or RNA synthesis, or intracellular levels of metabolites that are toxic to the fungal cell. (medscape.com)
  • Proliferating embryonic and cancer cells preferentially use aerobic glycolysis to support growth, a metabolic alteration commonly referred to as the "Warburg effect. (rupress.org)
  • Ca binding to the human red cell membrane: Characterization of membrane preparations and binding sites. (springer.com)
  • Passive transport is a naturally occurring phenomenon and does not require the cell to exert any of its energy to accomplish the movement. (oercommons.org)
  • At industrial level, microbial cells are often cultivated under conditions that can exert stress. (frontiersin.org)
  • In order to achieve high mechanical strength, the molecular structure should be such that the hydrated membrane contains narrow water channels, but these might decrease the proton conductivity. (mdpi.com)
  • The modeling work on the alkaline anion exchange membrane (AEM) fuel cell has been greatly facilitated by the rapid development of AEM fuel cell in recent years. (springer.com)
  • Y.S. Kang, Y. Heo, P. Kim, S.J. Yoo, Preparation and characterization of Cu-N-C electrocatalysts for oxygen reduction reaction in alkaline anion exchange membrane fuel cells. (springer.com)
  • Red blood cells have been chosen for this evaluation as a convenient model with nearly absent genomic and rapid posttranscriptional changes in function and minimal interferences between the ion fluxes in the external and intracellular membranes. (ahajournals.org)
  • However, determining the impact of plasma membrane-located aquaporins for whole plant or organ water transport efficiency proved difficult in this model plant because of its small size. (plantcell.org)
  • Aquaporins are a group of channels that specifically allow water molecules to cross the plasma membrane. (coursehero.com)
  • 2010). With respect to receptor-mediated endocytosis, a specific receptor on the cell surface binds to the extracellular molecule (Ligand). (ipl.org)
  • The cell membrane (also known as the plasma membrane or cytoplasmic membrane, and historically referred to as the plasmalemma ) is a biological membrane that separates the interior of all cells from the outside environment (the extracellular space). (wikipedia.org)
  • It separates the intracellular fluid, which is the fluid within the cells, from the extracellular fluid, which is found just outside of the cells. (wisegeek.com)
  • Traube had no direct evidence for the composition of this film, though, and incorrectly asserted that it was formed by an interfacial reaction of the cell protoplasm with the extracellular fluid. (wikipedia.org)
  • At the same time, the nutrients that the cell needs can pass through the membrane to the inside. (wisegeek.com)
  • These results were confirmed by fluorescence microscopy, where these bacteria presented permeabilization of their cytoplasmatic membranes after contact with the semi-purified fraction and pure compound. (frontiersin.org)
  • Compartmentalization of basal (1,4,5)IP3 was suggested by the fact that this material was stable in the presence of antimycin A, although this toxin completely blocked agonist stimulation of phospholipase C. However, subcellular fractionation as well as permeabilization of the cells with Staphylococcus aureus alpha-toxin failed to provide evidence for binding or sequestration of [3H](1,4,5)IP3 in AR4-2J cells. (biomedsearch.com)
  • A Northwestern University collaboration has developed a novel microlluidic device that allows for electroporation of stem cells during differentiation, making it possible to deliver molecules during this pivotal time in a cell's life. (thefreedictionary.com)
  • The function of a cell membrane is to control the movement of material into and out of the cell. (markedbyteachers.com)
  • Depletion of HK2, but neither HK1 nor pyruvate kinase M2, in GBM cells restored oxidative glucose metabolism and increased sensitivity to cell death inducers such as radiation and temozolomide. (rupress.org)
  • We demonstrate how a non-Gaussian shape of the DWI signal can originate solely due to the presence of cell membranes, with no diffusivity difference between the intra- and extra-cellular compartments. (ismrm.org)
  • We relate the cell membrane permeability and surface-to-volume ratio to experimentally relevant parameters, such as dispersive diffusivity and time-dependent kurtosis. (ismrm.org)
  • Flippases and Scramblases concentrate phosphatidyl serine , which carries a negative charge, on the inner membrane. (thefullwiki.org)
  • In 1890, an update to the Cell Theory stated that cell membranes existed, but were merely secondary structures. (wikipedia.org)
  • These structures, which can be visualized by electron microscopy or fluorescence microscopy , can be found on the inside of the membrane, the outside, or through-and-through . (bionity.com)
  • They include synapses , desmosomes , clathrin-coated pits , caveolaes , and different structures involved in cell adhesion. (bionity.com)
  • Since this behavior in the adipocyte, as in the erythrocyte, cannot be attributed to structures such as tight junctions, it must be explained on the basis of some physico-chemical feature of the cell membrane itself. (elsevier.com)
  • STRAND 3: CELLS - Students will describe basic concepts of structures and functions of cells. (google.com)
  • Cell membrane is the barrier that separates the inner environment of the cell from the outer environment. (markedbyteachers.com)
  • The cell membrane surrounds the protoplasm of a cell and, in animal cells, physically separates the intracellular components from , thereby serving a function similar to that of skin . (thefullwiki.org)
  • Cell theory has its origins in seventeenth century microscopy observations, but it was nearly two hundred years before a complete cell membrane theory was developed to explain what separates cells from the outside world. (wikipedia.org)
  • It is unanimously accepted that permeability of any specific molecule depends mainly on charge number, polarity, size, and to some extent, to the molar mass of the molecule. (sgmapps.com)
  • They interpreted this as meaning that to pass the cell membrane a molecule must be at least sparingly soluble in oil, their "lipoid theory of narcosis. (wikipedia.org)
  • So too do membranes have methods for checking and controlling what and how things can enter or leave a cell. (nursing.com)
  • The symposium on "Passive permea bility of telephone membranes" used to be consequently dedicated to those pas sive shipping methods and this booklet offers the continue ings of the convention. (cristinapascual.com)
  • DNA extracted from apoptotic cells and then separated by gel electrophoresis reveals a characteristic ladder pattern of low molecular weight DNA fragments. (thermofisher.com)
  • Molecular delivery of gear within the presence or absence of membranes is an ubiquitous phenomenon. (cristinapascual.com)
  • NATURE OF MEMBRANE ITSELF - ' its Molecular Makeup ' b. (miami.edu)
  • Although the results of this experiment were accurate, Fricke misinterpreted the data to mean that the cell membrane is a single molecular layer. (wikipedia.org)
  • The tobacco plasma membrane aquaporin NtAQP1 was used to elucidate this issue. (plantcell.org)
  • We chose the well-characterized tobacco plasma membrane-located aquaporin NtAQP1 for these studies. (plantcell.org)
  • Factors controlling the resealing of the membrane of human erythrocyte ghosts after hypotonic hemolysis. (springer.com)
  • 51 worldwide leading experts in the field of erythrocyte research contributed to this first book on transport processes in red blood cells. (worldcat.org)
  • Secondly, the values of the δΔF w→l indicate that the adipocyte membrane is less polar than the intestinal and gallbladder membranes but more polar than the membranes of Nitella and the erythrocyte. (elsevier.com)
  • By measuring the capacitance of erythrocyte solutions Fricke determined that the cell membrane was 3.3 nm thick. (wikipedia.org)
  • The increase of membrane fluidity with benzyl alcohol (BA) or chloroform (CF) resulted in a 3-fold decrease of MTX adsorption on HCT-116 R, exclusively. (nih.gov)
  • At 4 degrees C, drug accumulation measurements showed a decrease of MTX permeability in resistant membrane (42 pmol MTX/10(6) cells), restored with fluidizers (e.g., 342 pmol MTX/10(6) cells with BA). (nih.gov)
  • HO90 mM stress induced a cell-volume decrease for 3 s after stress observed at t=5 s before returning to almost normal size ( t=31 s), then a very discreet cytoplasmic retraction was observed. (nih.gov)
  • in artificial vesicles as well as in intact cells. (slideserve.com)
  • Phospholipid vesicles enriched with similar amounts of cholesterol also exhibit PCO2 approximate to 0.01 cm/s, identifying cholesterol as the major determinant of membrane PCO2. (unibas.ch)
  • into membrane vesicles. (miami.edu)