A cell line derived from cultured tumor cells.
Established cell cultures that have the potential to propagate indefinitely.
Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
The total amount (cell number, weight, size or volume) of tumor cells or tissue in the body.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.
Genes that inhibit expression of the tumorigenic phenotype. They are normally involved in holding cellular growth in check. When tumor suppressor genes are inactivated or lost, a barrier to normal proliferation is removed and unregulated growth is possible.
Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A malignant kidney tumor, caused by the uncontrolled multiplication of renal stem (blastemal), stromal (STROMAL CELLS), and epithelial (EPITHELIAL CELLS) elements. However, not all three are present in every case. Several genes or chromosomal areas have been associated with Wilms tumor which is usually found in childhood as a firm lump in a child's side or ABDOMEN.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
Proteins that are normally involved in holding cellular growth in check. Deficiencies or abnormalities in these proteins may lead to unregulated cell growth and tumor development.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.
Transplantation between animals of different species.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A malignant epithelial tumor with a glandular organization.
Neoplasms of the intracranial components of the central nervous system, including the cerebral hemispheres, basal ganglia, hypothalamus, thalamus, brain stem, and cerebellum. Brain neoplasms are subdivided into primary (originating from brain tissue) and secondary (i.e., metastatic) forms. Primary neoplasms are subdivided into benign and malignant forms. In general, brain tumors may also be classified by age of onset, histologic type, or presenting location in the brain.
A usually small, slow-growing neoplasm composed of islands of rounded, oxyphilic, or spindle-shaped cells of medium size, with moderately small vesicular nuclei, and covered by intact mucosa with a yellow cut surface. The tumor can occur anywhere in the gastrointestinal tract (and in the lungs and other sites); approximately 90% arise in the appendix. It is now established that these tumors are of neuroendocrine origin and derive from a primitive stem cell. (From Stedman, 25th ed & Holland et al., Cancer Medicine, 3d ed, p1182)
Tumors or cancer of the COLON.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
In vivo methods of screening investigative anticancer drugs, biologic response modifiers or radiotherapies. Human tumor tissue or cells are transplanted into mice or rats followed by tumor treatment regimens. A variety of outcomes are monitored to assess antitumor effectiveness.
The transfer of a neoplasm from one organ or part of the body to another remote from the primary site.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
DNA present in neoplastic tissue.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Tumors whose cells possess secretory granules and originate from the neuroectoderm, i.e., the cells of the ectoblast or epiblast that program the neuroendocrine system. Common properties across most neuroendocrine tumors include ectopic hormone production (often via APUD CELLS), the presence of tumor-associated antigens, and isozyme composition.
Experimentally induced mammary neoplasms in animals to provide a model for studying human BREAST NEOPLASMS.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Antibodies produced by a single clone of cells.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The milieu surrounding neoplasms consisting of cells, vessels, soluble factors, and molecules, that can influence and be influenced by, the neoplasm's growth.
Tumors or cancer of the human BREAST.
Experimental transplantation of neoplasms in laboratory animals for research purposes.
A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
Tumors or cancer of the LIVER.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS.
Elements of limited time intervals, contributing to particular results or situations.
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Benign and malignant central nervous system neoplasms derived from glial cells (i.e., astrocytes, oligodendrocytes, and ependymocytes). Astrocytes may give rise to astrocytomas (ASTROCYTOMA) or glioblastoma multiforme (see GLIOBLASTOMA). Oligodendrocytes give rise to oligodendrogliomas (OLIGODENDROGLIOMA) and ependymocytes may undergo transformation to become EPENDYMOMA; CHOROID PLEXUS NEOPLASMS; or colloid cysts of the third ventricle. (From Escourolle et al., Manual of Basic Neuropathology, 2nd ed, p21)
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
Tumors or cancer of the PANCREAS. Depending on the types of ISLET CELLS present in the tumors, various hormones can be secreted: GLUCAGON from PANCREATIC ALPHA CELLS; INSULIN from PANCREATIC BETA CELLS; and SOMATOSTATIN from the SOMATOSTATIN-SECRETING CELLS. Most are malignant except the insulin-producing tumors (INSULINOMA).
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
RNA present in neoplastic tissue.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
A sarcoma derived from deep fibrous tissue, characterized by bundles of immature proliferating fibroblasts with variable collagen formation, which tends to invade locally and metastasize by the bloodstream. (Stedman, 25th ed)
A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
A common neoplasm of early childhood arising from neural crest cells in the sympathetic nervous system, and characterized by diverse clinical behavior, ranging from spontaneous remission to rapid metastatic progression and death. This tumor is the most common intraabdominal malignancy of childhood, but it may also arise from thorax, neck, or rarely occur in the central nervous system. Histologic features include uniform round cells with hyperchromatic nuclei arranged in nests and separated by fibrovascular septa. Neuroblastomas may be associated with the opsoclonus-myoclonus syndrome. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2099-2101; Curr Opin Oncol 1998 Jan;10(1):43-51)
A primary malignant neoplasm of epithelial liver cells. It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. The cells may be uniform or markedly pleomorphic, or form GIANT CELLS. Several classification schemes have been suggested.
A cytologic technique for measuring the functional capacity of tumor stem cells by assaying their activity. It is used primarily for the in vitro testing of antineoplastic agents.
Tumors or cancers of the KIDNEY.
Tumors or cancer of the LUNG.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Tumors or cancer of the SKIN.
All tumors in the GASTROINTESTINAL TRACT arising from mesenchymal cells (MESODERM) except those of smooth muscle cells (LEIOMYOMA) or Schwann cells (SCHWANNOMA).
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Proteins prepared by recombinant DNA technology.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
A malignant form of astrocytoma histologically characterized by pleomorphism of cells, nuclear atypia, microhemorrhage, and necrosis. They may arise in any region of the central nervous system, with a predilection for the cerebral hemispheres, basal ganglia, and commissural pathways. Clinical presentation most frequently occurs in the fifth or sixth decade of life with focal neurologic signs or seizures.
Methods of investigating the effectiveness of anticancer cytotoxic drugs and biologic inhibitors. These include in vitro cell-kill models and cytostatic dye exclusion tests as well as in vivo measurement of tumor growth parameters in laboratory animals.
Diminished or failed response of an organism, disease or tissue to the intended effectiveness of a chemical or drug. It should be differentiated from DRUG TOLERANCE which is the progressive diminution of the susceptibility of a human or animal to the effects of a drug, as a result of continued administration.
The action of a drug in promoting or enhancing the effectiveness of another drug.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
A transplantable, poorly differentiated malignant tumor which appeared originally as a spontaneous breast carcinoma in a mouse. It grows in both solid and ascitic forms.
Addition of methyl groups to DNA. DNA methyltransferases (DNA methylases) perform this reaction using S-ADENOSYLMETHIONINE as the methyl group donor.
Experimentally induced neoplasms of CONNECTIVE TISSUE in animals to provide a model for studying human SARCOMA.
The worsening of a disease over time. This concept is most often used for chronic and incurable diseases where the stage of the disease is an important determinant of therapy and prognosis.
The rate dynamics in chemical or physical systems.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Mapping of the KARYOTYPE of a cell.
Methods which attempt to express in replicable terms the extent of the neoplasm in the patient.
Experimentally induced tumor that produces MELANIN in animals to provide a model for studying human MELANOMA.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Tumor suppressor genes located on the short arm of human chromosome 17 and coding for the phosphoprotein p53.
Tumors or cancer located in bone tissue or specific BONES.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
A sarcoma originating in bone-forming cells, affecting the ends of long bones. It is the most common and most malignant of sarcomas of the bones, and occurs chiefly among 10- to 25-year-old youths. (From Stedman, 25th ed)
Interruption or suppression of the expression of a gene at transcriptional or translational levels.
A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS.
Resistance or diminished response of a neoplasm to an antineoplastic agent in humans, animals, or cell or tissue cultures.
Transport proteins that carry specific substances in the blood or across cell membranes.
Tumors or cancer of the MAMMARY GLAND in animals (MAMMARY GLANDS, ANIMAL).
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Antineoplastic antibiotic obtained from Streptomyces peucetius. It is a hydroxy derivative of DAUNORUBICIN.
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
Experimentally induced tumors of the LIVER.
A general term for various neoplastic diseases of the lymphoid tissue.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Tumors or cancer of the URINARY BLADDER.
Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells such as the GOBLET CELLS.
Family of retrovirus-associated DNA sequences (ras) originally isolated from Harvey (H-ras, Ha-ras, rasH) and Kirsten (K-ras, Ki-ras, rasK) murine sarcoma viruses. Ras genes are widely conserved among animal species and sequences corresponding to both H-ras and K-ras genes have been detected in human, avian, murine, and non-vertebrate genomes. The closely related N-ras gene has been detected in human neuroblastoma and sarcoma cell lines. All genes of the family have a similar exon-intron structure and each encodes a p21 protein.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
A rare but highly lethal childhood tumor found almost exclusively in infants. Histopathologically, it resembles RHABDOMYOSARCOMA but the tumor cells are not of myogenic origin. Although it arises primarily in the kidney, it may be found in other parts of the body. The rhabdoid cytomorphology is believed to be the expression of a very primitive malignant cell. (From Holland et al., Cancer Medicine, 3d ed, p2210)
A selective increase in the number of copies of a gene coding for a specific protein without a proportional increase in other genes. It occurs naturally via the excision of a copy of the repeating sequence from the chromosome and its extrachromosomal replication in a plasmid, or via the production of an RNA transcript of the entire repeating sequence of ribosomal RNA followed by the reverse transcription of the molecule to produce an additional copy of the original DNA sequence. Laboratory techniques have been introduced for inducing disproportional replication by unequal crossing over, uptake of DNA from lysed cells, or generation of extrachromosomal sequences from rolling circle replication.
Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene.
An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
An inorganic and water-soluble platinum complex. After undergoing hydrolysis, it reacts with DNA to produce both intra and interstrand crosslinks. These crosslinks appear to impair replication and transcription of DNA. The cytotoxicity of cisplatin correlates with cellular arrest in the G2 phase of the cell cycle.
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
Neoplasms of the brain and spinal cord derived from glial cells which vary from histologically benign forms to highly anaplastic and malignant tumors. Fibrillary astrocytomas are the most common type and may be classified in order of increasing malignancy (grades I through IV). In the first two decades of life, astrocytomas tend to originate in the cerebellar hemispheres; in adults, they most frequently arise in the cerebrum and frequently undergo malignant transformation. (From Devita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2013-7; Holland et al., Cancer Medicine, 3d ed, p1082)
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A neoplasm composed entirely of GRANULOSA CELLS, occurring mostly in the OVARY. In the adult form, it may contain some THECA CELLS. This tumor often produces ESTRADIOL and INHIBIN. The excess estrogen exposure can lead to other malignancies in women and PRECOCIOUS PUBERTY in girls. In rare cases, granulosa cell tumors have been identified in the TESTES.
Membrane proteins encoded by the BCL-2 GENES and serving as potent inhibitors of cell death by APOPTOSIS. The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma.
Tumors or cancer of the STOMACH.
Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.
Highly proliferative, self-renewing, and colony-forming stem cells which give rise to NEOPLASMS.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Adherence of cells to surfaces or to other cells.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
The concentration of a compound needed to reduce population growth of organisms, including eukaryotic cells, by 50% in vitro. Though often expressed to denote in vitro antibacterial activity, it is also used as a benchmark for cytotoxicity to eukaryotic cells in culture.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
Soft tissue tumors or cancer arising from the mucosal surfaces of the LIP; oral cavity; PHARYNX; LARYNX; and cervical esophagus. Other sites included are the NOSE and PARANASAL SINUSES; SALIVARY GLANDS; THYROID GLAND and PARATHYROID GLANDS; and MELANOMA and non-melanoma skin cancers of the head and neck. (from Holland et al., Cancer Medicine, 4th ed, p1651)
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.
Substances that increase the risk of NEOPLASMS in humans or animals. Both genotoxic chemicals, which affect DNA directly, and nongenotoxic chemicals, which induce neoplasms by other mechanism, are included.
The local recurrence of a neoplasm following treatment. It arises from microscopic cells of the original neoplasm that have escaped therapeutic intervention and later become clinically visible at the original site.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
The artificial induction of GENE SILENCING by the use of RNA INTERFERENCE to reduce the expression of a specific gene. It includes the use of DOUBLE-STRANDED RNA, such as SMALL INTERFERING RNA and RNA containing HAIRPIN LOOP SEQUENCE, and ANTI-SENSE OLIGONUCLEOTIDES.
A tumor necrosis factor receptor subtype that has specificity for TUMOR NECROSIS FACTOR ALPHA and LYMPHOTOXIN ALPHA. It is constitutively expressed in most tissues and is a key mediator of tumor necrosis factor signaling in the vast majority of cells. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
A malignant solid tumor arising from mesenchymal tissues which normally differentiate to form striated muscle. It can occur in a wide variety of sites. It is divided into four distinct types: pleomorphic, predominantly in male adults; alveolar (RHABDOMYOSARCOMA, ALVEOLAR), mainly in adolescents and young adults; embryonal (RHABDOMYOSARCOMA, EMBRYONAL), predominantly in infants and children; and botryoidal, also in young children. It is one of the most frequently occurring soft tissue sarcomas and the most common in children under 15. (From Dorland, 27th ed; Holland et al., Cancer Medicine, 3d ed, p2186; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, pp1647-9)
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
A heterogeneous group of sporadic or hereditary carcinoma derived from cells of the KIDNEYS. There are several subtypes including the clear cells, the papillary, the chromophobe, the collecting duct, the spindle cells (sarcomatoid), or mixed cell-type carcinoma.
Manipulation of the host's immune system in treatment of disease. It includes both active and passive immunization as well as immunosuppressive therapy to prevent graft rejection.
The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.
A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Chemical substances, produced by microorganisms, inhibiting or preventing the proliferation of neoplasms.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
An anaplastic, highly malignant, and usually bronchogenic carcinoma composed of small ovoid cells with scanty neoplasm. It is characterized by a dominant, deeply basophilic nucleus, and absent or indistinct nucleoli. (From Stedman, 25th ed; Holland et al., Cancer Medicine, 3d ed, p1286-7)
The treatment of a disease or condition by several different means simultaneously or sequentially. Chemoimmunotherapy, RADIOIMMUNOTHERAPY, chemoradiotherapy, cryochemotherapy, and SALVAGE THERAPY are seen most frequently, but their combinations with each other and surgery are also used.
Methods for maintaining or growing CELLS in vitro.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.
The loss of one allele at a specific locus, caused by a deletion mutation; or loss of a chromosome from a chromosome pair, resulting in abnormal HEMIZYGOSITY. It is detected when heterozygous markers for a locus appear monomorphic because one of the ALLELES was deleted.
Transfer of a neoplasm from its primary site to lymph nodes or to distant parts of the body by way of the lymphatic system.
Agents that inhibit PROTEIN KINASES.
A benign epithelial tumor with a glandular organization.
A group of malignant tumors of the nervous system that feature primitive cells with elements of neuronal and/or glial differentiation. Use of this term is limited by some authors to central nervous system tumors and others include neoplasms of similar origin which arise extracranially (i.e., NEUROECTODERMAL TUMORS, PRIMITIVE, PERIPHERAL). This term is also occasionally used as a synonym for MEDULLOBLASTOMA. In general, these tumors arise in the first decade of life and tend to be highly malignant. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, p2059)
A protein-serine-threonine kinase that is activated by PHOSPHORYLATION in response to GROWTH FACTORS or INSULIN. It plays a major role in cell metabolism, growth, and survival as a core component of SIGNAL TRANSDUCTION. Three isoforms have been described in mammalian cells.
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
A connective tissue neoplasm formed by proliferation of mesodermal cells; it is usually highly malignant.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
A type of connective tissue neoplasm typically arising from intralobular stroma of the breast. It is characterized by the rapid enlargement of an asymmetric firm mobile mass. Histologically, its leaf-like stromal clefts are lined by EPITHELIAL CELLS. Rare phyllodes tumor of the prostate is also known.
Cytoplasmic proteins that bind estrogens and migrate to the nucleus where they regulate DNA transcription. Evaluation of the state of estrogen receptors in breast cancer patients has become clinically important.
Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)

Characterization of DNA binding, transcriptional activation, and regulated nuclear association of recombinant human NFATp. (1/67632)

BACKGROUND: NFATp is one member of a family of transcriptional activators whose nuclear accumulation and hence transcriptional activity is regulated in mammalian cells. Human NFATp exists as a phosphoprotein in the cytoplasm of naive T cells. Upon antigen stimulation, NFATp is dephosphorylated, accumulates in nuclei, and functions to regulate transcription of genes including those encoding cytokines. While the properties of the DNA binding domain of NFATp have been investigated in detail, biochemical studies of the transcriptional activation and regulated association with nuclei have remained unexplored because of a lack of full length, purified recombinant NFATp. RESULTS: We developed methods for expressing and purifying full length recombinant human NFATp that has all of the properties known to be associated with native NFATp. The recombinant NFATp binds DNA on its own and cooperatively with AP-1 proteins, activates transcription in vitro, is phosphorylated, can be dephosphorylated by calcineurin, and exhibits regulated association with nuclei in vitro. Importantly, activation by recombinant NFATp in a reconstituted transcription system required regions of the protein outside of the central DNA binding domain. CONCLUSIONS: We conclude that NFATp is a bona fide transcriptional activator. Moreover, the reagents and methods that we developed will facilitate future studies on the mechanisms of transcriptional activation and nuclear accumulation by NFATp, a member of an important family of transcriptional regulatory proteins.  (+info)

Differential expression of aquaporin 8 in human colonic epithelial cells and colorectal tumors. (2/67632)

BACKGROUND: The gene expression pattern in tumor cells differs from that in corresponding normal cells. In order to identify differentially expressed genes in colorectal tumors and normal colorectal epithelium, a differential display experiment was used to compare RNA expression in normal and tumor tissue samples. RESULTS: One gene fragment was expressed only in normal tissue and not, or to a much lesser extent, in the adenomas, carcinomas and cancer cell lines. The isolated gene fragment was identical to Aquaporin 8 (AQP8), a water channel protein. In situ hybridization demonstrated that AQP8 was expressed in the cells facing the lumen in the normal colonic epithelium. CONCLUSION: Our result suggests that the expression of AQP8 is a marker of normal proliferating colonic epithelial cells and suggest these cells to be involved in fluid transport in the colon.  (+info)

Developmental expression of survivin during embryonic submandibular salivary gland development. (3/67632)

BACKGROUND: The regulation of programmed cell death is critical to developmental homeostasis and normal morphogenesis of embryonic tissues. Survivin, a member of the inhibitors of apoptosis protein (IAP) family primarily expressed in embryonic cells, is both an anti-apoptosis and a pro-survival factor. Since our previous studies have demonstrated the importance of apoptosis during embryonic submandibular salivary gland (SMG) development, we postulated that survivin is a likely mediator of SMG epithelial cell survival. RESULTS: We investigated the developmental expression of survivin in Pseudoglandular (approximately E14), Canalicular (approximately E15) and Terminal Bud (approximately E17) Stage SMGs. We report a significant 26% increase in transcript levels between the Canalicular and Terminal Bud Stages. Immunohistochemical studies demonstrate nuclear-localized survivin protein in epithelial cells bounding forming lumina in Canalicular and Terminal Bud Stage SMGs. CONCLUSIONS: Survivin is known to be a pro-survival and anti-apoptotic factor. Given that survivin translocation into the nucleus is required for the induction of entry into the cell cycle and the inhibition of apoptosis, our demonstration of nuclear-localized survivin protein in presumptive ductal and proacinar lumen-bounding cells suggests that survivin may be a key mediator of embryonic SMG epithelial cell survival.  (+info)

p53-dependent apoptosis induced by proteasome inhibition in mammary epithelial cells. (4/67632)

We have examined the effects of inhibition of the 26S proteasome in a murine mammary cell line, KIM-2 cells using the peptide aldehyde inhibitor MG132. These studies have demonstrated a clear requirement for proteasome function in cell viability. Induction of apoptosis was observed following MG132 treatment in KIM-2 cells and this death was shown to be dependent on the cell actively traversing the cell cycle. KIM-2 cells were generated using a temperature sensitive T-antigen (Tag) and studies at the permissive temperature (33 degrees C) have shown that a Tag binding protein was essential for this apoptotic response. Studies in two additional cell lines, HC11, which is a mammary epithelial cell line carrying mutant p53 alleles and p53 null ES cells suggest that p53 is actively required for the apoptosis induced as a consequence of proteasome inhibition. These results suggest a pivotal role for the 26S proteasome degradation pathway in progression through the cell cycle in proliferating cells.  (+info)

Staurosporine-induced apoptosis of HPV positive and negative human cervical cancer cells from different points in the cell cycle. (5/67632)

In the present study, we compare the sensitivity of CaSki and HeLa cells (HPV positive, wild-type p53) and C33A cells (HPV negative, mutated p53) to a protein kinase inhibitor, the staurosporine (ST). We show that ST can reversibly arrest the three cervical-derived cell lines, either in G1 or in G2/M. Beyond certain ST concentrations or/and over 24 h exposure, the cells underwent apoptosis. This process took place in G1 and G2/M for C33A and CaSki plus HeLa cell lines, respectively. By using an in vitro cell-free system, we demonstrated that cytoplasmic extracts from apoptotic cells were sufficient to induce hallmarks of programmed cell death on isolated nuclei. Moreover, we found that only G2/M cytoplasmic extracts from viable CaSki and HeLa cells supplemented with ST, triggered apoptosis while exclusively G1 cytoplasmic fractions from C33A cells were efficient. Our study describes a possible involvement of the HPV infection or/and p53 status in this different ST-induced apoptosis susceptibility.  (+info)

Pro-caspase-3 overexpression sensitises ovarian cancer cells to proteasome inhibitors. (6/67632)

The ubiquitin-proteasome pathway plays a critical role in the degradation of several proteins involved in the cell cycle. Dysregulation of this pathway leads to inhibition of cellular proliferation and the induction of apoptosis. Ubiquitination and its downstream consequences have been investigated intensively as targets for the development of drugs for tumour therapy. Here we have investigated the mechanism of apoptosis induced by the proteasome inhibitors MG-132, lactacystin and calpain inhibitor I (ALLN), in the HEK 293 cell line and the ovarian cancer cell lines SKOV3 and OVCAR3. We have found strong caspase-3-like and caspase-6-like activation upon treatment of HEK 293 cells with MG-132. Using a tricistronic expression vector based on a tetracycline-responsive system we generated stable SKOV3 nd OVCAR3 cell lines with inducible expression of pro-caspase-3. Induction of pro-caspase-3 expression in normally growing cells does not induce apoptosis. However, in the presence of the proteasome inhibitors MG-132, lactacystin or ALLN we found that cells overexpressing pro-caspase-3 are rapidly targeted for apoptosis. Our results demonstrate that pro-caspase-3 can sensitise ovarian cancer cells to proteasome inhibitor-induced apoptosis, and a combination of these approaches might be exploited for therapy of ovarian and other cancers.  (+info)

A20 zinc finger protein inhibits TNF-induced apoptosis and stress response early in the signaling cascades and independently of binding to TRAF2 or 14-3-3 proteins. (7/67632)

A20 zinc finger protein is a negative regulator of tumor necrosis factor (TNF)-induced signaling pathways leading to apoptosis, stress response and inflammation. A20 has been shown to bind to TNF-receptor-associated factor 2 (TRAF2) and 14-3-3 chaperone proteins. Our data indicate that the zinc finger domain of A20 is sufficient and that neither TRAF2 nor 14-3-3 binding is necessary for the inhibitory effects of A20. Mutations in the 14-3-3 binding site of A20 did, however, result in a partial cleavage of A20 protein suggesting that 14-3-3 chaperone proteins may stabilize A20. Furthermore, we show that A20 acts early in TNF-induced signaling cascades blocking both TNF-induced rapid activation of c-Jun N-terminal kinase and processing of the receptor-associated caspase-8. Taken together our data indicate that the zinc finger domain of A20 contains all necessary functional domains required for the inhibition of TNF signaling and that A20 may function at the level of the receptor signaling complex.  (+info)

Apoptosis-inducing protein, AIP, from parasite-infected fish induces apoptosis in mammalian cells by two different molecular mechanisms. (8/67632)

AIP (apoptosis-inducing protein) is a protein purified and cloned from Chub mackerel infected with the larval nematode, Anisakis simplex, which induces apoptosis in various mammalian cells including human tumor cell lines. AIP has shown structural and functional homology to L-amino acid oxidase (LAO) which oxidizes several L-amino acids including L-lysine and AIP-induced apoptosis has been suggested to be mediated by H2O2 generated by LAO activity of AIP. In this study, we confirmed that recombinant AIP generated enough H2O2 in culture medium to induce rapid apoptosis in cells and this apoptosis was clearly inhibited by co-cultivation with antioxidants such as catalase and N-acetyl-cysteine. Surprisingly, however, we found that AIP still could induce H2O2-independent apoptosis more slowly than H2O2-dependent one in HL-60 cells even in the presence of antioxidants. In addition, the HL-60-derived cell line HP100-1, which is a H2O2-resistant variant, underwent apoptosis on treatment with AIP with a similar delayed time course. The latter apoptosis was completely blocked by addition of L-lysine to the culture medium, which is the best substrate of AIP as LAO, indicating that decreased concentration of L-lysine in the culture medium by AIP-treatment induced apoptosis. We also showed that the both apoptosis by AIP were associated with the release of cytochrome c from mitochondria and activation of caspase-9, and overexpressed Bcl-2 could inhibit both of the AIP-induced apoptosis. These results indicate that AIP induces apoptosis in cells by two distinct mechanisms; one rapid and mediated by H2O2, the other delayed and mediated by deprivation of L-lysine, both of which utilize caspase-9/cytochrome c system.  (+info)

Zhao, Q., Huo, X., Sun, F., Dong, R.[Retracted] Polyphenol‑rich extract of ,em,Saliva chinensis,/em, exhibits anticancer activity in different cancer cell lines, and induces cell cycle arrest at the G0/G1‑phase, apoptosis and loss of mitochondrial membrane potential in pancreatic cancer cells. Molecular Medicine Reports 23, no. 6 (2021): 462. https://doi.org/10.3892/mmr. ...
298168476 - EP 0869803 B1 20030416 - ALLOGENEIC PARACRINE CYTOKINE TUMOR VACCINES - [origin: WO9724132A1] The present invention provides a method of treating cancer comprising (a) obtaining a tumor cell line, (b) modifying the tumor cell line to render it capable of producing an increased level of a cytokine relative to the unmodified tumor cell line, and (c) administering the tumor cell line to a mammalian host having at least one tumor that is the same type of tumor as that from which the tumor cell line was obtained, wherein the tumor cell line is allogeneic and is not MHC-matched to the host. The present invention also provides a pancreatic tumor cell line, a method and medium for obtaining such a tumor cell line, and a composition comprised of cells of a purified pancreatic tumor cell line.[origin: WO9724132A1] The present invention provides a method of treating cancer comprising (a) obtaining a tumor cell line, (b) modifying the tumor cell line to render it capable of producing an increased level
Polyamines (PAs) are involved in regulation of cell growth and cellular survival by interacting with processes like translation, transcription or ion transport. It is described that polyamines can induce apoptosis in mesenchymal cell lines. The aim of our study was to analyze whether the physiological PAs (putrescine, spermidine or spermine) or the PA-derivate deoxyspergualin (DSG), a novel immunosuppressant, induce apoptosis in immunocompetent cells. Furthermore, we wanted to investigate which molecular mechanisms are involved in the execution of the cell death program. By means of flow cytometric analysis we found an induction of apoptosis by spermine (Spm) and DSG in quiescent and activated PBMCs, PHA generated lymphoblasts, and various tumor cell lines (Jurkat, SKW-3, U937). Moreover, DSG and Spm triggered apoptosis in human Fas-deficient cells and in cell lines MV4.11. and RS4.11., which are described to be resistant to apoptosis induction by many conventional chemotherapeutic agents. ...
Figure s2. Metformin (MET) effects on proliferation of human lung embryonic fibroblast cell line. Human lung embryonic fibroblast cell line was treated with an increasing concentrations of MET (0µmol-25µmol) for a period of 48 hours. Cells were subsequently fixed with ethanol and DNA content was used as a marker for proliferation rate determined by crystal violet staining. Results of three independent experiments are shown. Values represented are Mean±SEM.
Plant polyphenols have been highlighted not only as chemopreventive, but also as potential anticancer substances. Flavones are a subclass of natural flavonoids reported to have an antioxidant and anticancer activity. The aim of our study was to evaluate antioxidant and anticancer activity of seventeen trihydroxyflavone derivatives, including apigenin (API) and baicalein (BCL). Also, we wanted to find out if there is a correlation between those two effects. Cell growth inhibition testing was carried out using MTT assay in three different human cancer cell lines: lung (A549), breast (MCF-7) and brain epithelial (U87). Antioxidant activity was determined by the DPPH radical scavenging method. Thirteen trihydroxyflavones possessed anticancer activity against at least one tested cancer cell line. They were more active against the MCF-7 cell line, and the lowest activity was determined against the U87 cell line. The majority of compounds inhibited cancer cell growth at EC50 values between 10-50 µM. The most
2075 American ginseng extract (GE) has been shown to have anti-proliferative effects on breast cancer cells, possibly mediated by induction of the cyclin inhibitor protein p21. Little is known regarding the effects of GE in other cancer cell lines or its anti-cancer mechanism(s) of action. The goals of the present study were to determine the effects of water-extracted GE on the proliferation of human colon cancer cells and to examine the role of p21 in mediating these effects using wild-type and p21-null HCT116 cells (courtesy of Dr. B. Vogelstein). Cells were treated with a wide concentration-range of GE (0-2.0 mg/ml) every two days for a total of six days and total cell number was determined. Although proliferation was inhibited by GE in both wild-type and p21-null cells, the IC50 in p21-null cells (0.42 mg/ml) was nearly two fold lower than that in wild-type cells (0.8 mg/ml). Cells were then treated with an inhibitory concentration of GE (1.0 mg/ml) and viability was assessed by trypan blue ...
The systemic balance of angiogenic and anti-angiogenic factors has been proposed to play a key-role in primary tumor growth dependent growth suppression of secondary tumors. Despite the importance of the organ microenvironment to angiogenesis and microcirculation, the influence of a primary tumor on secondary bone tumors has not been investigated so far. Since breast cancer has a high propensity to spread to bone, we used an in vivo xenograft model to determine the impact of growing breast cancer cells (MCF-7) in the mammary fat pad on the microvascular properties of subsequently inoculated secondary breast cancer tumors in bone. Mice were either treated with a resection of the primary tumor (n = 10) or no surgery (n = 9) and intravital microscopy was performed over 25 days in bone tumors. Tumor growth in bone was temporarily suppressed by the primary tumor on days 10 and 14. While microvascular permeability and vascular diameter decreased in both groups over time, the presence of the primary ...
Distant metastasis and drug resistance represent the two major causes for breast cancer mortality. The majority of breast cancer patients who develop metastases also demonstrate multi-drug resistance. The presence of breast cancer stem cells (BCSCs) in these patients might be the underlying key elements for their clinical manifestations. Hence, targeting BCSCs represents an ideal goal for therapeutic development. Recently, cadherin 11 (CDH11) has been shown to be a key mediator for breast-to-bone metastasis; disrupting CDH11-mediated signaling represents an ideal target for drug development. Using bioinformatics approach, our team has identified two known small molecules (temporarily named RD-1 and RD-2) which can down-regulate CDH11 expression in different cancer cell lines including breast. In parallel, we have teamed up with an industrial partner developed who has produced a line of monoclonal anti-CDH11 antibodies with high specificity and neutralizing ability in suppressing breast cancer ...
Despite extensive investigations on the monocyte response to cancer cells, the data available are not sufficient to complete the picture of the molecular mechanisms underlying this phenomenon. When monocytes/macrophages contact a tumor, they activate an inflammatory response within a few hours (12, 40, 41). Nevertheless, monocytes become deactivated after their first exposure to cancer cells (12, 41). This kind of tolerance is characterized by a down-regulation in the expression of several cytokines, and might be termed cancer-induced tolerance. Indeed, the transcription of TNF-α, IL-12, and other proinflammatory molecules is negatively regulated following the initial monocyte contact with tumor cells (12).. Our current observations confirm these previous reports regarding down-regulation of inflammatory responses when monocytes are re-exposed to a particular tumor cell line. In our hands, the inflammatory responses of human monocytes exposed to six different human cancer cell lines were ...
F the soft agar colony formation in comparison with vector control cells exposed to arsenite for eight weeks. A single explanation of these information is the
Previously (Liu et al., Cancer Res., 56: 3371-3379, 1996), we isolated a novel serine protease-like gene-Normal Epithelial Cell Specific-1 (NES1)-that is expressed in normal mammary epithelial cells but is down-regulated in most breast cancer cell lines. Here, we demonstrate that stable expression of NES1 in the NES1-negative MDA-MB-231 breast cancer cell line suppressed the oncogenicity as revealed by inhibition of the anchorage-independent growth and tumor formation in nude mice. Fluorescence in situ hybridization localized the NES1 gene to chromosome 19q13.3, a region that contains genes for related proteases (including the prostate-specific antigen) and is rearranged in human cancers. Similar to breast cancer cell lines, prostate cancer cell lines also lacked NES1 mRNA and protein expression. Together, these results strongly suggest a tumor-suppressor role for NES1 in breast and prostate cancer.. ...
Pazopanib and Sorafenib delay tumor growth in vivo and prolong the survival of mice bearing intracranial human medulloblastomaIn a orthotopic xenograft mouse mo
Effect of Cav-1 expression in prostate cancer cells on in vitro lymphangiogenesis(A) LEC differentiation into tube-like structures was investigated by plating t
Panelists Suresh S. Ramalingam, MD; Marina Garassino, MD; Benjamin Besse, MD, PhD; and Giorgio Scagliotti, MD, PhD, explain what promising molecular targets are emerging for non–small cell lung cancer, particularly |em|HER2|/em| and |em|MET|/em|.
Preclinical validation of potential therapeutic targets via the use of in vivo models is traditionally regarded as an obligatory step of anticancer drug development, but it is also considered a problematic issue. There is now increasing concern that what is still deemed a successful end point at the preclinical level-positive performance of a drug in xenografts of different human cancer cell lines-is in fact not predictive of a compounds efficacy in the clinical setting (44). The obvious objection is that immortalized cancer cells, which are commonly used in xenograft experiments, have been adapted to grow on plastic in the laboratory for decades and thus exhibit a genetic drift, a biologic compliance, and phenotypic features different from original cancers in patients.. Besides this evident flaw, another (often underestimated) drawback of such an approach is that the catalog of currently available cell lines is inevitably finite and possibly poor for some tumor types. The main reason for this ...
Preclinical validation of potential therapeutic targets via the use of in vivo models is traditionally regarded as an obligatory step of anticancer drug development, but it is also considered a problematic issue. There is now increasing concern that what is still deemed a successful end point at the preclinical level-positive performance of a drug in xenografts of different human cancer cell lines-is in fact not predictive of a compounds efficacy in the clinical setting (44). The obvious objection is that immortalized cancer cells, which are commonly used in xenograft experiments, have been adapted to grow on plastic in the laboratory for decades and thus exhibit a genetic drift, a biologic compliance, and phenotypic features different from original cancers in patients.. Besides this evident flaw, another (often underestimated) drawback of such an approach is that the catalog of currently available cell lines is inevitably finite and possibly poor for some tumor types. The main reason for this ...
This study is for patients with breast, prostate, ovarian, non-small cell lung (NSCL) or bladder cancer who have failed potentially curative treatments or for whose disease a curative treatment does not exist.. OGX-427 is a second-generation ASO that inhibits expression of Hsp27. Hsp27 is one of the heat shock proteins. Hsp27 increases with cell stress, including cytotoxic chemotherapy, radiation therapy and hormone therapy and has been shown to inhibit cell death. Thus, decreasing Hsp27 as a cancer therapy is attractive as a therapy as it should result in down regulation of pathways implicated in cancer progression and development of resistance to treatment.. A number of in vitro and in vivo pharmacological studies have demonstrated that OGX-427 has single-agent activity in reducing Hsp27, inhibiting cell growth and inducing cell death in several human cancer cell lines. OGX-427 has also demonstrated chemosensitizing activity in studies using cell lines and animal models in combination with ...
In this study, we showed that SPARCL1 suppresses the proliferation, migration, invasion, and anchorage-independent growth of colon cancer cells (Fig. 1). The expression of SPARCL1 also induces the differentiation of colon cancer cells (Fig. 4). The results are consistent with previously conducted in vitro studies (8, 22). Here, we further report the in vivo findings obtained using xenograft animal models. In the subcutaneous xenograft mouse model, the expression of SPARCL1 retarded tumor growth, which points to its anti-proliferation potential (Supplementary Fig. S4). We used an intrasplenic injection mouse model mimicking liver metastasis of CRC at the later stages. This was based on the anatomy assumption that colon cancer cells migrate mostly through vena porta hepatic (23). The liver metastasis animal model indicated that SPARCL1 significantly reduces the liver metastasis by RKO cells (Fig. 2). These in vitro and in vivo studies validate that the expression of SPARCL1 has potential ...
Hypoxia plays an important role in the resistance of tumour cells to chemotherapy. However, the exact mechanisms underlying this process are not well understood. Moreover, according to the cell lines, hypoxia differently influences cell death. The study of the effects of hypoxia on the apoptosis induced by 5 chemotherapeutic drugs in 7 cancer cell types showed that hypoxia generally inhibited the drug-induced apoptosis. In most cases, the effect of hypoxia was the same for all the drugs in one cell type. The expression profile of 93 genes involved in apoptosis as well as the protein level of BCL-2 family proteins were then investigated. In HepG2 cells that are strongly protected against cell death by hypoxia, hypoxia decreased the abundance of nearly all the pro-apoptotic BCL-2 family proteins while none of them are decreased in A549 cells that are not protected against cell death by hypoxia. In HepG2 cells, hypoxia decreased NOXA and BAD abundance and modified the electrophoretic mobility of BIMEL. BIM
We used the cancer-intrinsic property of oncogene-induced DNA damage as the base for a conditional synthetic lethality approach. To target mechanisms important for cancer cell adaptation to genotoxic stress and thereby to achieve cancer cell-specific killing, we combined inhibition of the kinases ATR and Wee1. Wee1 regulates cell cycle progression, whereas ATR is an apical kinase in the DNA-damage response. In an orthotopic breast cancer model, tumor-selective synthetic lethality of the combination of bioavailable ATR and Wee1 inhibitors led to tumor remission and inhibited metastasis with minimal side effects. ATR and Wee1 inhibition had a higher synergistic effect in cancer stem cells than in bulk cancer cells, compensating for the lower sensitivity of cancer stem cells to the individual drugs. Mechanistically, the combination treatment caused cells with unrepaired or under-replicated DNA to enter mitosis leading to mitotic catastrophe. As these inhibitors of ATR and Wee1 are already in phase ...
Background:. Esophageal cancer (EC) is an aggressive malignancy with increasing incidence and poor outcome (1). New therapeutic strategies are urgently required. The phosphatidylinositol 3-kinase (PI3K)/AKT signal pathway has been documented as a central hub for the malignant behaviors of cancer cells (2). However, the functional role and therapeutic effect of PI3K/AKT inhibitors in esophageal cancer metastasis is underappreciated.. Aim:. We aim to study the clinical significance of PI3K/AKT signaling pathway in EC metastasis and evaluate the therapeutic effect of PI3K/AKT-targeted therapy.. Methods:. A highly invasive cancer cell line (KYSE410-I3) was established by serial selection of the EC cells invading through the matrigel-coated Boyden chamber. Cell migration and invasion were determined using Boyden chamber migration and invasion assays. Western blot and immunohistochemistry were used to detect protein expressions in cell lysates and in a tissue microarray containing 40 pairs of ...
Single Cell Profiling of Circulating Tumor Cells: Transcriptional Heterogeneity and Diversity from Breast Cancer Cell Lines. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
An isogenic cell line ATCC CCL-185IG was created by using CRISPR gene editing technology, and contains EML4-ALK fusion variant 1 (E13; A20).
An isogenic cell line ATCC CCL-185IG was created by using CRISPR gene editing technology, and contains EML4-ALK fusion variant 1 (E13; A20).
Ideal cancer targets should have the following features: (a) they play an essential role in carcinogenesis, and/or are required for the maintenance of cancer cell phenotype, and/or are survival proteins that confer resistance to cancer cells against apoptosis; (b) they are overexpressed in cancer cells, which is associated with a poor prognosis of patient survival; (c) inhibition of their expression or activity induces growth suppression and/or apoptosis in cancer cells, but not in normal cells, achieving a potential therapeutic window; and (d) it is druggable, that is, it is an enzyme (e.g., kinase) or a cell surface molecule (e.g., membrane-bound receptor) that can be easily screened for small-molecule inhibitors or being targeted by a specific antibody (27, 28). In this study, we validated SAG, a dual-function protein with antioxidant and ligase activities, as a potential anticancer target. We showed here that (a) SAG is overexpressed in several human cancers originated from different ...
We here show that the physiological estrogen metabolite 2-Methoxyestradiol (2-ME) has a very strong growth inhibitory effect on cell lines from different solid cancer types. The tumors investigated were hepatocellular carcinome (HCC), pancreatic cancer, and lung cancer. All three tumor types present with a very poor prognosis, which did not improve significantly the last 20 years in spite of new operation techniques, new anticancer drugs, or new molecular approaches. Using several different cell lines of each cancer type we studied, we could confirm a generalized phenomenon of cancer growth inhibition by 2-ME. We found up to 90% growth inhibition in all cell lines with the exception of one pancreatic cancer cell line. This effect could even be increased using combination therapies of 2-ME and Gemcitabine, 5-FU, Taxol or a monoclonal antibody against the EGF receptor. We found an additive growth inhibition when all of these anticancer agents were combined with 2-ME. Our studies on lung cancer ...
We here show that the physiological estrogen metabolite 2-Methoxyestradiol (2-ME) has a very strong growth inhibitory effect on cell lines from different solid cancer types. The tumors investigated were hepatocellular carcinome (HCC), pancreatic cancer, and lung cancer. All three tumor types present with a very poor prognosis, which did not improve significantly the last 20 years in spite of new operation techniques, new anticancer drugs, or new molecular approaches. Using several different cell lines of each cancer type we studied, we could confirm a generalized phenomenon of cancer growth inhibition by 2-ME. We found up to 90% growth inhibition in all cell lines with the exception of one pancreatic cancer cell line. This effect could even be increased using combination therapies of 2-ME and Gemcitabine, 5-FU, Taxol or a monoclonal antibody against the EGF receptor. We found an additive growth inhibition when all of these anticancer agents were combined with 2-ME. Our studies on lung cancer ...
Raji Xenograft Model. What is a Xenograft?. Development of an anti-cancer therapeutic requires intense, well planned studies that follow a streamlined path for success. Primary studies are performed in an in vitro setting that allows for high throughput screening and analysis of multiple compounds of interest. This method enables a focused compound screening approach of multiple cell lines within a specific cancer type, or a divergent approach across a broad range of cancer types. Ultimately, in vitro screening results need to be confirmed in an animal model due to in vitro inadequacies of cells cultured on plastic, as this method is far removed from the microenvironment of a tumor.. As the logical next step in therapeutic development is the administration of the test compound in a living animal, a cell line derived xenograft model (CDX) is created by inoculating human cancer cell lines in test animals. The injected cell lines grow into established tumors, thus, permitting efficacy studies of ...
Interleukin-15 (IL-15) is a common γ-chain cytokine that plays a significant role in the activation and proliferation of T and NK cells and holds great potential in fighting cancer. We have previously shown that bioactive IL-15 in vivo comprises a complex of the IL-15 chain with the soluble or cell-associated IL-15 receptor alpha chain that are together termed heterodimeric IL-15 (hetIL-15). We investigated the anti-tumor efficacy of hetIL-15 in several mouse cancer models. Repeated injections of hetIL-15 were effective in delaying tumor growth in the MC38 colon carcinoma and the B16 melanoma models. The beneficial effects of hetIL-15 therapy included a significantly reduced onset of lung metastasis in 4T1 breast cancer-bearing mice. We study the mechanisms of antitumor effects of hetIL-15 in several tumor models. hetIL-15 administration promoted a dramatic increase of tumor infiltration and persistence of CD8+ T cells, including tumor specific T cells, and resulted in an increased CD8+/Treg ...
Development of novel and effective therapeutics for treating various cancers is probably the most congested and challenging enterprise of pharmaceutical companies. Diverse drugs targeting malignant and nonmalignant cells receive clinical approval each year from the FDA. Targeting cancer cells and nonmalignant cells unavoidably changes the tumor microenvironment, and cellular and molecular components relentlessly alter in response to drugs. Cancer cells often reprogram their metabolic pathways to adapt to environmental challenges and facilitate survival, proliferation, and metastasis. While cancer cells dependence on glycolysis for energy production is well studied, the roles of adipocytes and lipid metabolic reprogramming in supporting cancer growth, metastasis, and drug responses are less understood. This Review focuses on emerging mechanisms involving adipocytes and lipid metabolism in altering the response to cancer treatment. In particular, we discuss mechanisms underlying cancer-associated ...
IL-13Rα2 chain has been shown to play a unique role in tumor biology. It is overexpressed in a variety of primary tumor cell cultures and tumor cell lines (13, 15-18, 25, 34), whereas normal cells including lymphoid cells, endothelial cells, and astrocytes (13, 15, 17, 26) do not express or express low levels of this cytokine receptor chain. Recent studies have demonstrated that overexpression of IL-13Rα2 chain in certain breast and pancreatic cancer cell lines resulted into loss of tumorigenicity, whereas unmodified control tumor cells formed enlarging tumor nodules when injected in immunodeficient mice (46). In another study, Terabe et al. (47) have demonstrated that the soluble form of IL-13Rα2 chain (5) can modulate immune environment and shift Th2 phenotype to a dominant Th1 phenotype. This shift resulted into resistance of 15-12RM cell-derived tumor recurrence. Thus, further investigation of the role of IL-13Rα2 chain in tumor immunology and targeting has become extremely ...
Abstract: The synthesis of an innovative delivery system for targeted cancer therapy which combines the drug controlled release ability of Molecularly Imprinted Polymers (MIPs) with magnetic properties of magnetite is described herein. In the present study, an easy and smart synthetic strategy, involving new engineered precipitation photo-polymerization, was developed with the aim to obtain Magnetic Molecularly Imprinted Polymers (MMIPs) for 9H-carbazole derivative sustained delivery in cancer treatment. Both in vitro drug release and cytotoxicity studies on different cancer cell lines, such as HeLa and MCF-7, were performed in order to evaluate the controlled release ability and the potential application as a drug carrier in targeted cancer therapy. The synthesized polymeric materials have shown not only good selective recognition and controlled release properties, but also high magnetic responding capacity. The performed cytotoxicity studies highlighted the high inhibitory activity against the ...
Researchers at the University of Washington have blended the past with the present in the fight against cancer, synthesizing a promising new compound from an ancient Chinese remedy that uses cancer cells rapacious appetite for iron to make them a target.
Bao et al. uncover that the subpopulation of CD133+ cells isolated from mind tumors exhibit CICs houses, are refractory to chemo- or radiotherapy, and market
Certain compounds in cancerous cells - derivatives of the chemical phenanthridine - end up disrupting the spindle, as the team found, preventing cell chromosomes from splitting, and stopping the cell from dividing.. The growth of the cancer is essentially mitosis out of control, but with the division halted thanks to the new protein mechanism, these cancer cells can die off instead of spreading throughout the body.. According to the mechanism we discovered, the faster cancer cells proliferate, the faster and more efficiently they will be eradicated, says Cohen-Armon.. This technique was tested with positive results on a variety of tumour types in human cancer cell cultures, including breast, lung, ovary, colon, pancreas, blood, and brain cancer.. This is one of many researches currently exploring ways to kill off cancer cells with minimum damage to the body.. Last year, a protein called ProAgio that could trigger cancer cell death was identified from researchers from Georgia State University. ...
BioAssay record AID 103734 submitted by ChEMBL: Compound was tested for cancer cell line growth inhibition against human breast (MCF-7) cell line.
Pdf is an enzyme that, during protein production, removes a modification called an N-formyl group from the first amino acid, a methionine, in the protein chain. While work began on the development of antibiotics against what was thought to be a bacterial-exclusive enzyme, genome-based data searches identified several classes of Pdf-like sequences in parasites, plants and mammals. Subsequent studies showed that the Pdfs were active both in culture and in the living organism, thus potentially derailing the usefulness of these antibiotics for specifically combating infectious agents. In previous studies, Scheinberg and colleagues had found that actinonin had an antiproliferative effect on human cancer cell lines and on tumor growth in a mouse model. They theorized this growth inhibitory activity might be related to actinonin s inhibition of human Pdf ...
CD146, a marker of endothelial cells, promotes tumor progression of many cancers including melanoma and the prostate. Strikingly, several lines of evidence suggest that it is a suppressor of breast cancer (BC) progression. In addition, not only the ligand(s) has not been identified, but CD146-downstream mechanisms remain unknown. Here, we report a novel molecular mechanism by which CD146 acts as a suppressor of breast tumor growth. A novel transcriptional target of CD146-suppressed BC, TimpV, the only endogenous protein inhibitor known for metallocarboxypeptidases, was identified and validated using novel validated Enhanced Green Fluorescent Protein (EGFP)-inducible systems of CD146 expression in both, the weakly and the highly invasive BC cell lines MCF-7 and MDA-MB-231, respectively. CD146/TimpV association was validated by quantitative PCR and immunoblotting experiments in a range of BC cells. In functional experiments, both CD146 induction and siRNA experimental approaches revealed that, ...
Circulating Tumor Cells Circulating Tumor Cells (CTC) • Metastasis: the spread of cancer from its primary site to other places in the body. Metastatic disease is the most common cause of cancer-related death in patients with solid tumors. Metastasis Process CTCs • Standard definition of a CTC: - Epithelial tumor cell which has adopted genetic mutations that enable migration through the basement membrane and the extracellular matrix, and is free to circulate in the blood stream - confirmed by: 1) visualization of an intact nucleus (using DAPI, 4′,6-diamidino-2phenylindole, a DNA-binding fluorescent stain) 2) expression of cytokeratin 3) lack of expression of the white blood cell marker, CD45, the leukocyte-common antigen gene - Invasive tumor cells tend to loose their epithelial antigens by the epithelial to mesenchymal transition (EMT) process, so CTCs cannot be diagnosed based on the expression of epithelial-specific transcripts or antigens Clinical Usefulness • Many metastatic lesions ...
Ski, the transforming protein of the avian Sloan-Kettering retrovirus, inhibits transforming growth factor-β (TGF-β)/Smad signaling and displays both pro-oncogenic and anti-oncogenic activities in human cancer. Inhibition of TGF-β signaling is likely responsible for the pro-oncogenic activity of Ski. We investigated the mechanism(s) underlying the tumor suppressor activity of Ski and found that Ski suppressed the activity of the Hippo signaling effectors TAZ and YAP to inhibit breast cancer progression. TAZ and YAP are transcriptional coactivators that can contribute to cancer by promoting proliferation, tumorigenesis, and cancer stem cell expansion. Hippo signaling activates the the Lats family of kinases, which phosphorylate TAZ and YAP, resulting in cytoplasmic retention and degradation and inhibition of their transcriptional activity. We showed that Ski interacted with multiple components of the Hippo pathway to facilitate activation of Lats2, resulting in increased phosphorylation and ...
Macrophages within the tumor microenvironment facilitate angiogenesis and extracellular-matrix breakdown and remodeling and promote tumor cell motility. Recent studies reveal that direct communication between macrophages and tumor cells leads to invasion and egress of tumor cells into the blood vess …
As combining therapeutic agents with different action mechanisms may enhance efficacy, YSC-02, an oncolytic adenovirus with multi targets was loaded with five different genes, which were designed with the expectation that different action mechanisms would cooperate. In spite of concerns regarding many APIs, YSC-02 was constructed to be an adenovirus-based anti-cancer drug. By using our own established mouse model system suitable for efficient adenoviral infection and replication, immune activity as well as survival potential could be precisely estimated for anti-cancer drug efficacy. YSC-02 was designed to decrease tumor cell survival, metastasis and to increase tumor cell death, and immune activation. It is composed of five different target genes, including one fused form and two shRNAs, but each of these genes functions is closely linked to produce the maximal antitumor effect. YSC-02 is like an organic complex designed to be applied primarily to heterogeneous liver cancer and melanoma. The ...
BioAssay record AID 83769 submitted by ChEMBL: The compound was tested in vitro for antiproliferative activity against HT-29 tumor cell lines.
Background: Medicinal plants, especially examples rich in polyphenolic compounds, have been suggested to be chemopreventive on account of antioxidative properties. Punica granatum (PG) (pomegranate) is a well known fruit in this context, but its cytotoxicity in cancer cells has not been extensively studied. Here, we investigated the antiproliferative properties of a peel extract of PG from Iran in different human cancer cells. Materials and Methods: A methanolic extract of pomegranate peel (PPE) was prepared. Total phenolic content(TPC) and total flavonoid conetnt (TFC) were determined by colorimetric assays. Antioxidant activity was determined by DPPH radical scavenging activity. The cytotoxicity of different doses of PPE (0, 5, 20, 100, 250, 500, |TEX|$1000{\mu}g/ml$|/TEX|) was evaluated by MTT assays with A549 (lung non small cell cancer), MCF-7 (breast adenocarcinoma), SKOV3 (ovarian cancer), and PC-3 (prostate adenocarcinoma) cells. Results: Significant (P|0.01) or very significant (P|0.0001)
TY - JOUR. T1 - Enhancement of sensitivity of human lung cancer cell line to TRAIL and gefitinib by IGF-1R blockade. AU - Lee, Yoon Jin. AU - Park, Mi Young. AU - Young, Ae Kang. AU - Kwon, Sung Youn. AU - Yoon, Ho Il. AU - Lee, Jae Ho. AU - Lee, Choon Taek. PY - 2007/7. Y1 - 2007/7. N2 - Background: TRAIL is a cytokine that selectively induces apoptosis in various cancer cell lines. Gefitinib is new targeted drug applied in lung cancer that selectively inhibits EGFR tyrosine kinase. However, lung cancers have shown an initial or acquired resistance to these drugs. This study examined the effect of IGF-1R and its blockade on enhancing the sensitivity of lung cancer cell lines to TRAIL and gefitinib. Methods: Two lung cancer cell lines were used in this study. NCI H460 is very sensitive to TRAIL and gefitinib. On the other hand, A549 shows moderate resistance to TRAIL and gefitinib. The IGF-1R blockade was performed using adenoviruses expressing the dominant negative IGF-1R and shRNA to IGF-1R ...
Thuja, a bioactive derivative of Thuja occidentalis, has been widely accredited for its antitumorigenic potential (25,26). Although reports have verified the anticancer effect of this remedy (8-10), detailed reports elucidating the molecular mechanisms underlying the anticancer effect of thuja are needed. The present study demonstrated that the antitumorigenic effect of thuja on breast cancer cells was not a placebo effect as the placebo (potentized hydro-alcoholic solution)-treated cells failed to induce significant cell death when compared to the control cells. The present study further revealed that thuja asserted its effects by re-orienting the molecular choreography of cancer cells. Importantly, the preferential induction of the cytotoxic effects in breast cancer cells, as compared to normal cells, suggests a safe and non-toxic therapeutic opportunity.. It was shown that thuja is a potent inducer of apoptosis in mammary epithelial carcinoma cells, with a more pronounced effect in ...
TY - JOUR. T1 - Determinants of drug response in a cisplatin-resistant human lung cancer cell line. AU - Fujiwara, Yasuhiro. AU - Sugimoto, Yoshikazu. AU - Kasahara, Kazuo. AU - Bungo, Masami. AU - Yamakido, Michio. AU - Tew, Kenneth D.. AU - Saijo, Nagahiro. PY - 1990/5. Y1 - 1990/5. N2 - To elucidate the mechanism(s) of cisplatin resistance, we have characterized a human non-small cell lung cancer cell line ( PC-9 CDDP) selected from the wild type (PC-9) for acquired resistance to cisplatin. PC-9 CDDP demonstrated 28-fold resistance to cisplatin, with cross resistance to other chemotherapeutic drugs including chlorambucil (×6.3), melphalan (×3.7) and 3-[(4-amino-2-methyl-5-pyrimidinyl)]methyl-1-(2-chloroethyl)-1-nitrosourea (ACNU) (×3.9). There was no expression of mdr-1 mRNA in either wild-type or resistant cells. The mRNA and protein levels of glutathione S-transferase (GST) π were similar in the two lines. A GST-μ isozyme was present in equal amounts and the activities of ...
Lung cancer is the commonest type of cancer with the highest fatality rate worldwide. There is continued research that experiments on drug development for lung cancer patients by assessing their responses to chemotherapeutic treatments to select novel targets for improved therapies. This study aims to analyze the anticancer drug sensitivity in human lung cancer cell lines by using machine learning techniques. The data for this analysis is extracted from the National Cancer Institute (NCI). This experiment uses 408,291 human small molecule lung cancer cell lines to conclude. The values are drawn from describing the raw viability values for 91 human lung cancer cell lines treated with 354 different chemical compounds and 432 concentration points tested in each replicate experiments. Our analysis demonstrated the data from a considerable amount of cell lines clustered by using Simple K-means, Filtered clustering and by calculating sensitive drugs for each lung cancer cell line. Additionally, our analysis
The aim of this study was the evaluation of the effects of strawberry anthocyanin extracts treatment on two in vitro models of murine breast cancer cell line, trying to detect a specific pathway (AMP-activated protein kinase, or AMPK) through which strawberries exert their anticancer activity. The anticancer
There is increasing evidence for the involvement of miRNAs in mammalian biology and breast cancer. For instance, the levels of MiR-206 have been found to be higher in ERalpha-negative MB-MDA-231 cells than in ERalpha-positive MCF-7 cells [12], and enforced expression of miR-125a or miR-125b leads to coordinate suppression of ERBB2 and ERBB3 in the human breast cancer cell line SKBR3 [13]. Furthermore, MiR-27b, which is expressed in MCF-7 cells, may be one of the causes of high expression of the drug-metabolising enzyme CYP1B1 in cancerous tissues [14]. Finally, as a tumor suppressor in breast cancer cells, miR-17-5p regulates breast cancer cell proliferation by inhibiting the translation of AIB1 mRNA [15].. Research on the roles of BCSC-related miRNAs in breast cancer has great significance. Ponti [16] isolated tumorigenic breast cancer cells with stem/progenitor cell properties from a breast cancer cell line, and Huang [17] screened side population (SP) cells from a breast cancer cell line. ...
Focal adhesion kinase, FAK is a 125 kDa nonreceptor tyrosine kinase that localizes to focal adhesions. FAK is overexpressed in human tumors and regulates cellular adhesion and survival signaling. We have shown previously that the dominant-negative FAK, C-terminal FAK-CD, caused detachment and apoptosis in human breast cancer cells, and that overexpression of an activated form of Src tyrosine kinase or epidermal growth factor receptor, EGFR, suppressed FAK-CD induced apoptotic effects in breast cancer cells. In the present study, we studied the effect of a novel FAK inhibitor, TAE226 (Novartis, Inc.), on the breast cancer cell lines. We used stable breast cancer cell lines overexpressing Src (MCF-7-Src and BT474-Src) or overexpressing EGFR (BT474-EGFR), and control breast cancer cell lines for the treatment with different doses of TAE226 drug. The detachment and apoptosis caused by TAE226 was analyzed and compared with the effect of the dominant-negative adenoviral FAK-CD. The TAE226 drug caused ...
The genus Stachys belongs to Lamiaceae family with about 300 species and worldwide distribution. In the present study, the cytotoxic activity of four fractions of different Satchys species (S. byzatina C. Koch., S. inflata Benth., S. setifera Ten. and S. persica Gmel.), has been investigated against HT-29 (colon carcinoma), Caco-2 (colorectal adenocarcinoma), T-47D (breast ductal carcinoma) and NIH-3T3 (Swiss mouse embryo fibroblast) cell lines by MTT test. The samples were extracted by percolation method with four solvents; petroleum ether (60-80 ºC), chloroform, ethyl acetate and 80% aqueous methanol, susseccively. All cell lines were cultured in proper medium. Concentrations of 62.5-750 μg/mL from partition fractions of all samples, dissolved in 1% (v/v) DMSO were tested on each cell line. Cells with no treatment and methotrexate were examined as negative and positive controls, respectively. Cell viability was determined by MTT assay. Some fractions showed good cell inhibitory activity with IC50S.
Research highlights: {yields} PPAR{gamma} ligands increased the rate of apoptosis and inhibition of proliferation in ovarian cancer cells. {yields} PPAR{gamma} ligands induced p63 and p73 expression, but not p53. {yields} p63 and p73 leads to an increase in p21 expression and apoptosis in ovarian cancer cells with treatment PPAR{gamma} ligands. {yields} These findings suggest that PPAR{gamma} ligands suppressed growth of ovarian cancer cells through upregulation of p63 and p73. -- Abstract: Peroxisome proliferator-activated receptor gamma (PPAR{gamma}) agonists, including thiazolidinediones (TZDs), can induce anti-proliferation, differentiation, and apoptosis in various cancer cell types. This study investigated the mechanism of the anticancer effect of TZDs on human ovarian cancer. Six human ovarian cancer cell lines (NIH:OVCAR3, SKOV3, SNU-251, SNU-8, SNU-840, and 2774) were treated with the TZD, which induced dose-dependent inhibition of cell growth. Additionally, these cell lines exhibited ...
To address the role of β(1) integrins in pancreatic cancer progression, we stably knocked down β(1) integrin subunit expression in human FG-RFP pancreatic cancer cells using lentiviral-based RNA interference. We then examined the effects of β(1) integrin subunit knockdown on pancreatic cancer cell adhesion, migration and proliferation on tumor microenvironment-specific extracellular matrix proteins in vitro and on tumor progression in vivo using a clinically relevant fluorescent orthotopic mouse model of pancreatic cancer. Knockdown of the β(1) integrin subunit inhibited cell adhesion, migration and proliferation on types I and IV collagen, fibronectin and laminin in vitro. In vivo, knockdown of the β(1) integrin subunit reduced primary tumor growth by 50% and completely inhibited spontaneously occurring metastasis. These observations indicate a critical role for the β(1) integrin subunit in pancreatic cancer progression and metastasis in particular. Our results suggest the β(1) integrin
Using a broad panel of 44 human breast cancer cell lines and 3 immortalized breast epithelial lines, we have shown that HER2 amplification status was a strong predictor of response to dacomitinib, with IC50 values below 1 μmol/L in the vast majority of HER2-amplified lines. MDA-MB-453 and UACC-732 were the only HER2-amplified lines that were resistant to dacomitinib. Of note, these 2 lines have some of the lowest levels of HER2 DNA copy number by comparative genomic hybridization (data not shown) and HER2 mRNA among the HER2-amplified lines. In addition, these 2 lines express low baseline levels of total and phosphorylated HER2 (25) and total and phosphorylated EGFR (data not shown). These 2 cell lines were also previously shown to be resistant to trastuzumab (25) and lapatinib (25, 26). These findings may have potential clinical implications for selecting the patients for clinical trials with dacomitinib.. Dacomitinib showed an antiproliferative activity superior to trastuzumab in vitro. The ...
There has been a long‐standing debate over the role of LNs in promoting cancer malignancy. Some clinical evidence has suggested that metastasis to the LNs in breast cancer patients is strongly associated with distant organ metastasis, poor disease‐free survival, and shorter overall survival (Rouzier et al, 2002; Ran et al, 2010). Although breast cancer cell‐induced lymphangiogenesis in TDLNs is important to distant organ metastasis in mouse model, there is no direct evidence to demonstrate that breast cancer cells metastasized to LNs was required for further distant organ metastasis (Hirakawa et al, 2007; Shibata et al, 2008). Several clinical trials showed no survival benefits for patients underwent lymphadenectomy (Gervasoni et al, 2007). Thus, whether TDLNs involved in the progression of systemic metastasis remain controversial (Ran et al, 2010; Pereira et al, 2015). Using a syngeneic mouse model, we observed that breast tumor cells derived from TDLN gained higher malignancy compared ...
HMGB3 silence inhibits breast cancer cell proliferation and tumor growth by interacting with hypoxia-inducible factor 1alpha Jun Gu, Tao Xu, Qin-Hua Huang, Chu-Miao Zhang, Hai-Yan ChenDepartment of Health Check-Up Center, Jinshan Hospital, Fudan University, Shanghai 201508, Peoples Republic of ChinaBackground: Breast cancer is the most common malignant tumor that affects women with higher incidence. High-mobility group box 3 (HMGB3) plays critical functions in DNA repair, recombination, transcription and replication. This study aimed to investigate the effects of HMGB3 silence on mammosphere formation and tumor growth of breast cancer.Methods: LV5-HMGB3 and LV3-siHMGB3 vectors were transfected into MCF10A, MDA-MB-231, HCC1937, ZR-75-1 and MCF7 cells. Cell counting kit-8 (CCK-8) assay was used to evaluate cell proliferation. Xenograft tumor mice model was established by injection of MDA-MB-231. qRT-PCR and western blot were used to examine the expression of Nanog, Sox2 and OCT-4. Mammosphere forming
ARHI is a Ras-related imprinted gene that inhibits cancer cell growth and motility. ARHI is downregulated in the majority of breast cancers, and loss of its expression is associated with its progression from ductal carcinoma in situ (DCIS) to invasive disease. In ovarian cancer, re-expression of ARHI induces autophagy and leads to autophagic death in cell culture; however, ARHI re-expression enables ovarian cancer cells to remain dormant when they are grown in mice as xenografts. The purpose of this study is to examine whether ARHI induces autophagy in breast cancer cells and to evaluate the effects of ARHI gene re-expression in combination with paclitaxel. Re-expression of ARHI was achieved by transfection, by treatment with trichostatin A (TSA) or by a combination of TSA and 5-aza-2-deoxycytidine (DAC) in breast cancer cell cultures and by liposomal delivery of ARHI in breast tumor xenografts. ARHI re-expression induces autophagy in breast cancer cells, and ARHI is essential for the induction of
Brain metastasis is an increasingly common complication for breast cancer patients; approximately 15- 30% of breast cancer patients develop brain metastasis. However, relatively little is known about how these metastases form, and what phenotypes are characteristic of cells with brain metastasizing potential. In this study, we show that the targeted knockdown of MMP-1 in breast cancer cells with enhanced brain metastatic ability not only reduced primary tumor growth, but also significantly inhibited brain metastasis. Two variants of the MDA-MB-231 human breast cancer cell line selected for enhanced ability to form brain metastases in nude mice (231-BR and 231-BR3 cells) were found to express high levels of matrix metalloproteinase-1 (MMP-1). Short hairpin RNA-mediated stable knockdown of MMP-1 in 231-BR and 231-BR3 cells were established to analyze tumorigenic ability and metastatic ability. Short hairpin RNA-mediated stable knockdown of MMP-1 inhibited the invasive ability of MDA-MB 231 variant cells
HE4, also known as WFDC2, is a useful biomarker for ovarian cancer when either used alone or in combination with CA125. HE4 is also overexpressed in endometrial cancer (EC), but its function in cancer cells is not clear. In this study, we investigate the role of HE4 in EC progression. An HE4-overexpression system was established by cloning the HE4 prototypic mRNA variant (HE4-V0) into a eukaryotic expression vector. Following transfection, stable clones in two EC cell lines were selected. The effects of HE4 overexpression on cell growth and function were measured with the use of cell proliferation assay, matrigel invasion, and soft agar gel colony formation assays. HE4-induced cancer cell proliferation in vivo was examined in a mouse xenograft model. HE4 overexpression significantly enhanced EC cell proliferation, matrigel invasion, and colony formation in soft agar. Moreover, HE4 overexpression promoted tumor growth in the mouse xenograft model. HE4 overexpression enhanced several malignant phenotypes
TY - JOUR. T1 - Amphiregulin is a potent mitogen in human pancreatic cancer cells. T2 - Correlation with patient survival. AU - Yokoyama, M.. AU - Ebert, M.. AU - Funatomi, H.. AU - Friess, H.. AU - Buchler, M. W.. AU - Johnson, G. R.. AU - Korc, Murray. PY - 1995. Y1 - 1995. N2 - The epidermal growth factor (EGF) receptor (EGFR) is activated by EGF and other EGF-like growth factors, including amphiregulin (AR). We characterized the localization and mitogenic action of AR in T3M4 and COLO-357 human pancreatic cancer cell lines and determined whether the presence of AR in human pancreatic cancers correlates with patient survival. Both T3M4 and COLO-357 cells were found to be extremely sensitive to AR, one-half maximal stimulation occurring at a concentration of 70 and 50 pM, respectively. The magnitude of the stimulatory effect was greater with AR than with EGF. Both cell lines exhibited AR immunostaining, which was present in a variable manner in the cytoplasm, nucleus and nucleoli. ...
Cell lines and culture conditions. Human breast cancer cell lines (MDA-MB-231 and MCF-7) and 293T were purchased from the American Type Culture Collection (ATCC). These cell lines were authenticated at ATCC before purchase by standard short tandem repeat DNA-typing methodology. The murine mammary carcinoma cell line E0771 was purchased from BeNa Culture Collection. The Py8119 cell line was provided by Suling Liu (Fudan University, Shanghai, China). The MDA-MB-231, E0771, and 293T cell lines were maintained in Dulbeccos modified Eagle medium (DMEM; Invitrogen) supplemented with 10% FBS (Invitrogen Corp.). The MCF-7 cell line was maintained in Eagles minimum essential medium (Invitrogen Corp.) supplemented with 10% FBS and 0.01 mg/ml human recombinant insulin. The Py8119 cell line was maintained in F12K nutrient media (HyClone) supplemented with 5% FBS. Each cell line was cultured in standard medium as recommended by ATCC. All cells were incubated at 37°C in a humidified incubator containing 5% ...
Most human cancers have CNVs, which impact upon gene dosage through loss or gain of whole chromosomes or chromosome segments (Hanahan and Weinberg 2011). Previous studies have described CNVs in PC3 and LNCaP using targeted techniques, such as exome sequencing. However, WGS, together with continuously updated gene annotations, offers improved detection of copy number changes (Meynert et al. 2014; Belkadi et al. 2015; Warr et al. 2015).. CNVs were identified using the R package cn.mops (Klambauer et al. 2012). In particular, we wished to identify genes that are lost in PC3 and LNCaP. The absence of this information can misinform even the most well-designed in vitro or cell line xenograft experiment (e.g., where a gene in an important pathway is lost). In the context of CNV analysis, we were interested in identifying putative homozygous deletions (CNV = 0; CNV0 events), i.e., genes that are inactivated by partial or complete gene deletion. To inform this analysis, we also considered the ...
The invasive, mesenchymal phenotype of CD44posCD24neg breast cancer cells has made them a promising target for eliminating the metastatic capacity of primary tumors. It has been previously demonstrated that CD44neg/lowCD24pos breast cancer cells lack the ability to give rise to their invasive CD44posCD24neg counterpart. Here we demonstrate that noninvasive, epithelial-like CD44posCD24pos cells readily give rise to invasive, mesenchymal CD44posCD24neg progeny in vivo and in vitro. This interconversion was found to be dependent upon Activin/Nodal signaling. Breast cancer cell lines were sorted into CD44posCD24pos and CD44posCD24neg populations to evaluate their progeny for the expression of CD44, CD24, and markers of a mesenchymal phenotype. The populations, separated by fluorescence activated cell sorting (FACS) were injected into immunocompromised mice to evaluate their tumorigenicity and invasiveness of the resulting xenografts. CD24 expression was dynamically regulated in vitro in all evaluated breast
The invasive, mesenchymal phenotype of CD44posCD24neg breast cancer cells has made them a promising target for eliminating the metastatic capacity of primary tumors. It has been previously demonstrated that CD44neg/lowCD24pos breast cancer cells lack the ability to give rise to their invasive CD44posCD24neg counterpart. Here we demonstrate that noninvasive, epithelial-like CD44posCD24pos cells readily give rise to invasive, mesenchymal CD44posCD24neg progeny in vivo and in vitro. This interconversion was found to be dependent upon Activin/Nodal signaling. Breast cancer cell lines were sorted into CD44posCD24pos and CD44posCD24neg populations to evaluate their progeny for the expression of CD44, CD24, and markers of a mesenchymal phenotype. The populations, separated by fluorescence activated cell sorting (FACS) were injected into immunocompromised mice to evaluate their tumorigenicity and invasiveness of the resulting xenografts. CD24 expression was dynamically regulated in vitro in all evaluated breast
TY - JOUR. T1 - Rad51b activity and cell cycle regulation in response to DNA damage in breast cancer cell lines. AU - Lee, Phoebe S.. AU - Fang, Jun. AU - Jessop, Lea. AU - Myers, Timothy. AU - Raj, Preethi. AU - Hu, Nan. AU - Wang, Chaoyu. AU - Taylor, Philip R.. AU - Wang, Jianjun. AU - Khan, Javed. AU - Jasin, Maria. AU - Chanock, Stephen J.. PY - 2014/10/12. Y1 - 2014/10/12. N2 - Common genetic variants mapping to two distinct regions of RAD51B, a paralog of RAD51, have been associated with breast cancer risk in genome-wide association studies (GWAS). RAD51B is a plausible candidate gene because of its established role in the homologous recombination (HR) process. How germline genetic variation in RAD51B confers susceptibility to breast cancer is not well understood. Here, we investigate the molecular function of RAD51B in breast cancer cell lines by knocking down RAD51B expression by small interfering RNA and treating cells with DNA-damaging agents, namely cisplatin, hydroxyurea, or ...
Podocalyxin (gene name PODXL) is a CD34-related sialomucin implicated in the regulation of cell adhesion, migration and polarity. Upregulated expression of podocalyxin is linked to poor patient survival in epithelial cancers. However, it is not known if podocalyxin has a functional role in tumor progression. We silenced podocalyxin expression in the aggressive basal-like human (MDA-MB-231) and mouse (4T1) breast cancer cell lines and also overexpressed podocalyxin in the more benign human breast cancer cell line, MCF7. We evaluated how podocalyxin affects tumorsphere formation in vitro and compared the ability of podocalyxin-deficient and podocalyxin-replete cell lines to form tumors and metastasize using xenogenic or syngeneic transplant models in mice. Finally, in an effort to develop therapeutic treatments for systemic cancers, we generated a series of antihuman podocalyxin antibodies and screened these for their ability to inhibit tumor progression in xenografted mice. Although deletion of
Fibroblast growth factors (FGFs) and their high-affinity receptors contribute to the autocrine growth stimulation in several human malignancies. Here, we describe that FGF18 expression is up-regulated in 34/38 colorectal tumours and is progressively enhanced during colon carcinogenesis reaching very high levels in carcinoma. Moreover, our data suggest that FGF18 affects both tumour cells and tumour microenvironment in a pro-tumorigenic and pro-metastatic way. Addition of recombinant FGF18 to the culture media of slowly growing colorectal tumour cell lines LT97 and Caco-2 stimulated proliferation. Phosphorylation of externally regulated kinase 1/2 and S6 was increased already 5 min after growth factor addition. SW480 cells, endogenously producing large amounts of FGF18, were not affected in this setting, but recombinant FGF18 supported tumour cell survival under conditions of serum starvation. Down-modulation of endogenous FGF18 production by small interference RNA (siRNA) significantly reduced ...
Genome-wide genetic information in about 1,000 cancer cell lines is available on COSMIC DB(Sanger Center, UK), and on recent NGS analyses(Klijin C et al., Nat Biotechnol 2015); however, among them, only 28 cell lines are derived from GC. Since driver gene mutation frequency in a certain cancer is often less than 5%, the establishment of cell lines from each patient to be analyzed is desired for functional selection of driver gene mutations. Furthermore, almost all of the 28 GC cell lines were established many years ago, thereby, the clinical and pathological information is insufficient. The wait is on for the establishment of new GC cell lines, especially from metastatic sites after therapy. Peritoneal metastasis is most frequent in GCs, especially diffuse-type GCs. In 2016, we successfully established 13 diffuse-type GC cell lines from the cancer ascites of 12 patients. In collaboration with the Division of Genetics, we have totally established 84 diffuse-type GC cell lines(National ...
In addition to serving as a physical support, the extracellular matrix (ECM) actively influences cell behavior. However, the definitive effects of different chemical structures present in the ECM on cell behavior remain obscure. The current study aimed to investigate the effects of different chemical structures present in the ECM on cellular physiology using the ovarian cancer cell line SKOV-3 as a model. Self-assembled monolayers (SAMs) with different chemical modifications, including methyl (-CH|sub|3|/sub|), hydroxyl (-OH), amino (-NH|sub|2|/sub|), carboxyl (-COOH), and mercapto (-SH) groups, were used as microenvironmental models to explore the effects of different structures on SKOV-3 cells. The cell morphology, cell adhesion, cytotoxicity, and functional alterations in cancer cells cultured on different SAMs were analyzed. The results showed that SKOV-3 cells cultured on -NH|sub|2|/sub| surfaces exhibited the largest contact area, whereas those on -CH|sub|3|/sub| surfaces exhibited the smallest
Analysis of H3K4me3 and H3K27me3 bivalent promotors in HER2+ breast cancer cell lines reveals variations depending on estrogen receptor status and significantly correlates with gene expression.
The apoptosis-stimulating protein of p53 (ASPP) family comprises three members, namely, ASPP1, ASPP2, and iASPP. They regulate the promotive effect of p53 on apoptosis. Breast cancer (BC) remains as one of the leading causes of cancer or cancer-related mortality among women. However, the relationship between the ASPP family members and p53, as well as the dissemination and expression pattern of ASPP family members in p53+ BC, has not been elucidated. Our objectives are to detect the expression of ASPP family members in p53+ BC cell lines and determine its significance in tumor cell apoptosis. The mRNA expression of ASPP family members in five p53+ BC cell lines was detected through RT-PCR and assayed using Quality-one software. The p53 protein expression was detected by immunohistochemistry. Afterward, the apoptosis indices of the five BC cell lines were detected by flow cytometry. The iASPP mRNA was expressed in Bcap-37, MCF-7, and HBL-100. Compared with the human peripheral blood mononuclear cells,
Breast cancer, which derives from the epithelium of the mammary glands, is one of the most common cancers diagnosed in women globally. To date, the authors of many studies have reported that the deregulation of microRNAs (miRNAs) plays a crucial role in the occurrence, development, and metastasis of tumors. Here, we discovered that miR-660-5p was upregulated in the breast cancer cell lines MCF7 and MDA-MB-231 compared with that in the normal control cell line CCD-1095Sk. We then inhibited the expression of miR-660-5p to investigate its biological function in cancer development, progression, and metastasis. We determined the changes in the levels of expression of transcription factor CP2 (TFCP2) and CDKN1A to further clarify the specific mechanism involved. The results showed that downregulation of miR-660-5p significantly suppressed the proliferation, migration, and invasion of MCF7 breast cancer cell. Moreover, inhibition of miR-660-5p promoted cell cycle G1 arrest and reduced apoptosis in ...
Our objective was to evaluate the cancer suppression activity of extracts from a commercial variety of yellow-fleshed peach Rich Lady (RL) and a red-fleshed plum Black Splendor (BS) and identify the phenolic fractions that may possess potential as chemopreventive and/or chemotherapeutic natural compounds. The peach RL extract effectively inhibited the proliferation of the estrogen-independent MDA-MB-435 breast cancer cell line. The concentration to inhibit 50% of cell proliferation (IC(50)) was approximately 42 mg/L for this cell line compared to an IC(50) of approximately 130 and approximately 515 mg/L for the noncancerous breast cell line MCF-10A and the estrogen-dependent breast cancer cell line MCF-7, respectively. Similarly, BS extracts showed greater effects on MDA-MB-435 cells as compared to the other breast cancer or the normal breast cell lines. In general, BS extracts were less effective than RL extracts. Within all RL and BS fractions, fraction 3 (F3, flavonoids) and fraction 4 ...
By using sophisticated gene sequencing methods to demonstrate a regulatory link between the stem cell factor Lin28 and the signaling molecule bone morphogenic protein 4, Yale researchers are creating a path for developing new targeted ovarian cancer therapies.. Researchers at Yale School of Medicine have identified a key link between stem cell factors that fuel ovarian cancers growth and patient prognosis. The study, which paves the way for developing novel targeted ovarian cancer therapies, is published online in the current issue of Cell Cycle.. Lead author Dr. Yingqun Huang, associate professor in the Department of Obstetrics, Gynecology & Reproductive Sciences, and her colleagues have demonstrated a connection between two concepts that are revolutionizing the way cancer is treated.. First, the cancer stem cell idea suggests that at the heart of every tumor there is a small subset of difficult-to-identify tumor cells that fuel the growth of the bulk of the tumor. This concept predicts that ...
"Isolation and characterization of spheroid cells from human malignant melanoma cell line WM-266-4". Tumour Biology. 30 (5-6): ... Wang Q, Chen ZG, Du CZ, Wang HW, Yan L, Gu J (September 2009). "Cancer stem cell marker CD133+ tumour cells and clinical ... CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastoma, neuronal and glial stem cells, ... "CD133 negative glioma cells form tumors in nude rats and give rise to CD133 positive cells". International Journal of Cancer. ...
"Phosphorylation of pyruvate kinase and glycolytic metabolism in three human glioma cell lines". Tumour Biology. 12 (6): 339-52 ... In tumor cells, PKM2 is mainly in the dimeric form and has, therefore, been termed Tumor M2-PK. The quantification of Tumor M2- ... Thus, PKM2 is a regulator of LPS- and tumor-induced PD-L1 expression on macrophages and dendritic cells as well as tumor cells ... such as normal proliferating cells, embryonic cells, and especially tumor cells. Two isozymes are encoded by the PKM gene: PKM1 ...
... ability to induce apoptosis in cancer cell lines, which could play an important role in developing treatments for tumor cells ... and developed a cell line. Previously, cells cultured from other human cells would only survive for a few days. Cells from ... is an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line is ... The cell line was named "HeLa" after the first two letters in Lacks's name. At first, the HeLa cell line was said to be named ...
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Expression was also detected in many epithelial tumor cell lines. Two transcript variants encoding distinct isoforms have been ... Tumor suppressor candidate 3 is a protein that in humans is encoded by the TUSC3 gene. This gene is a candidate tumor ... "Entrez Gene: TUSC3 tumor suppressor candidate 3". Pak BJ, Park H, Chang ER, et al. (1998). "Differential display analysis of ... Cell. 12 (1): 101-11. doi:10.1016/S1097-2765(03)00243-0. PMID 12887896. Anderson NL, Polanski M, Pieper R, et al. (2004). "The ...
"NCI-60 DTP Human Tumor Cell Line Screen". Developmental Therapeutics Program, National Cancer Institute. Archived from the ... "60-cell line screen" The complete chemical synthesis of halichondrin B was achieved by Yoshito Kishi and colleagues at Harvard ... these authors also reported the exquisite anticancer activity of halichondrin B against murine cancer cells both in culture and ...
ARK5 is often overexpressed in multiple myeloma cell lines. ARK5 promotes tumor cell survival under regulation by Akt. ARK5 ... also inhibits ARK5 and reduces proliferation of multiple myeloma and mantle cell lymphoma cell lines. NUAK1 has been shown to ... 2004). "ARK5 is a tumor invasion-associated factor downstream of Akt signaling". Mol. Cell. Biol. 24 (8): 3526-35. doi:10.1128/ ... Suzuki A, Kusakai G, Kishimoto A, Lu J, Ogura T, Esumi H (Sep 2003). "ARK5 suppresses the cell death induced by nutrient ...
... in breast tumors and six breast cancer cell lines". Tumour Biology. 36 (10): 8201-6. doi:10.1007/s13277-015-3546-4. PMID ... Expression is typically limited to meiotic cells, but overexpression occurs in some cancer cell lines. PLZF, a transcription ... producing multinucleated cells and tumors in vivo when overexpressed. When cells overexpressing Aurora C were treated with ... Although all of the Aurora kinases are overexpressed in many cancer cell lines, only Aurora A and C possess oncogenic activity ...
"A human pituitary tumor-derived folliculo-stellate cell line". J Clin Endocrinol Metab. 85 (3): 1180-1187. doi:10.1210/jc.85.3. ... This tumor is thought to be derived from the parenchymal cells of the posterior lobe of the pituitary gland, called pituicytes ... Some researchers believe that they arise from the folliculostellate cells in the anterior lobe of the pituitary. As such, it is ... Inoue K, Couch EF, Takano K, Ogawa S (1999). "The structure and function of folliculo-stellate cells in the anterior pituitary ...
The anti-tumor activity of withaferin A was tested on human prostate cancer cell line, PC-3 and confirmed in PC-3 xenografts in ... "Growth inhibition of human tumor cell lines by withanolides from Withania somnifera leaves". Life Sciences. 74 (1): 125-132. ... It has been suggested that sensitization of cancer cells to radiation is due to the inhibition of NF-κB. It exhibits anti-tumor ... Withaferin A has been shown to enhance radiation-induced apoptosis in certain cell lines. However, its mechanism of action on ...
Sep 2016). "Top-down proteomic characterization of DAOY medulloblastoma tumor cell line". EuPA Open Proteomics. 12: 13-21. doi: ... Sep 2003). "Protein profiles of medulloblastoma cell lines DAOY and D283: identification of tumor-related proteins and ... DAOY is a widely used human primary medulloblastoma cell line. It has epithelial morphology and was obtained from a 4-year-old ... Srivastava VK, Nalbantoglu J (Oct 2008). "Flow cytometric characterization of the DAOY medulloblastoma cell line for the cancer ...
It also has strong cytotoxicity against various tumor cell lines. Because of its potent biological activities, kendomycin has ...
The widely used angiotensin-II-responsive steroid-producing cell line H295R was originally isolated from a tumor diagnosed as ... On microscopic examination, the tumor usually displays sheets of atypical cells with some resemblance to the cells of the ... Wang T, Rainey WE (March 2012). "Human adrenocortical carcinoma cell lines". Molecular and Cellular Endocrinology. 351 (1): 58- ... "Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid ...
... near the promoters of tumor suppressor genes have been documented in specific tumor cell lines. In the case of the tumor ... In both human normal and tumor cell lines, YPEL3 has been shown to be a p53-inducible gene. Two putative p53 binding sites have ... YPEL3 has growth inhibitory effects in normal and tumor cell lines. One of five family members (YPEL1-5), YPEL3 was named in ... Campisi J (2005). "Senescent Cells, Tumor Suppression, and Organismal Aging: Good Citizens, Bad Neighbors". Cell. 120 (4): 513- ...
2-methoxyestradiol is cytotoxic to several tumor cell lines, binds to the colchicine site of tubulin, inducing the formation of ... Hemiasterlin A and hemiasterlin B show potent activity against the P388 cell line and inhibit cell division by binding to the ... was described to be the main cause of the inhibition of cell division and of tumor cell death in paclitaxel treated cells. ... cell shape maintenance, polarity, cell signaling and mitosis. They play a critical role in cell division by involving in the ...
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"Mutation analyses of 268 candidate genes in human tumor cell lines". Genomics. 74 (3): 352-64. doi:10.1006/geno.2001.6551. PMID ... March 2020). "SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor". Cell ... doi:10.1016/j.cell.2020.02.052. PMC 7102627. PMID 32142651. Lay summary - Deutsches Primatenzentrum GmbH. Rahman N, Basharat Z ... This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen- ...
The single layer of cells lining the tubules are cuboidal with a scant to moderate amount of cytoplasm. In some areas they may ... Microscopically the tumor shows closely packed small tubular structures in edematous stroma. The tubules show considerable ... It is thought to result from displacement and implantation of renal tubular cells, as this entity in kidney transplant ... Aug 2002). "Derivation of nephrogenic adenomas from renal tubular cells in kidney-transplant recipients". N Engl J Med. 347 (9 ...
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HEK 293 became one of the most used cell lines worldwide, coming close to the use of the HeLa cell line. In 1974 van der Eb and ... In 1974 van der Eb became a lector of fundamental tumor virology. In 1979 he was named professor of fundamental tumor virology ... With Graham's contributions in transfection of the cells with the adenovirus 5 this led to the cell line of HEK 293 cells. ... he created the PER.C6 cell line with enhanced traceability compared to the HEK 293 line, this time coming from retinal cells. ...
It is upregulated in several human colon and prostate cancer cell lines and tissues. Knockdown of mLST8 prevented mTORC ... "mLST8 Promotes mTOR-Mediated Tumor Progression". PLOS ONE. 10 (4): e0119015. doi:10.1371/journal.pone.0119015. PMC 4408021. ... 2002). "Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control". Mol. Cell. 10 ... Cell. 11 (4): 895-904. doi:10.1016/S1097-2765(03)00114-X. PMID 12718876. Jacinto E, Loewith R, Schmidt A, et al. (2004). " ...
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Aberrant splice variants expressed in tumor cell lines have also been described. In humans, mutations in BIN1 have been ... "A Role for the Putative Tumor Suppressor Bin1 in Muscle Cell Differentiation". Mol. Cell. Biol. 18 (1): 566-75. doi:10.1128/MCB ... Cell. 8 (10): 2003-15. doi:10.1091/mbc.8.10.2003. PMC 25662. PMID 9348539. Kadlec L, Pendergast AM (1997). "The amphiphysin- ... Cell Sci. 116 (Pt 10): 1937-48. doi:10.1242/jcs.00403. ISSN 0021-9533. PMID 12668730. Andersson B, Wentland MA, Ricafrente JY, ...
"Isolation of two human tumor epithelial cell lines from solid breast carcinomas". Nat. Genet. 11 (2): 198-200. doi:10.1038/ ... "Stable interaction between the products of the BRCA1 and BRCA2 tumor suppressor genes in mitotic and meiotic cells". Mol. Cell ... In breast cancer cell lines, there is an inverse correlation of BRCA1 protein levels with miR-182 expression. Thus it appears ... Another microRNA known to reduce expression of BRCA1 in ovarian cancer cells is miR-9. Among 58 tumors from patients with stage ...
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Brown VL, Proby CM, Barnes DM, Kelsell DP (July 2002). "Lack of mutations within ST7 gene in tumour-derived cell lines and ... Sivasundaram K, Suzuki H, Seto M, Hosokawa Y (2004). "Mutational analysis of the ST7 gene in human myeloid tumor cell lines". ... "Chromosome 7 suppresses indefinite division of nontumorigenic immortalized human fibroblast cell lines KMST-6 and SUSM-1". ... Vincent JB, Petek E, Thevarkunnel S, Kolozsvari D, Cheung J, Patel M, Scherer SW (September 2002). "The RAY1/ST7 tumor- ...
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A tumor cell line with defective δ-catenin, low levels of E-cadherin and poor cell-to-cell adhesion could be restored to normal ... F9 embryonal carcinoma cells are similar to the P19 cells shown in Figure 1 and normally have cell-to-cell adhesion mediated by ... However, decreases in this adhesion ability of the cell has been linked to metastasis and tumor progression. In normal cells, α ... "Knockdown of Sec6 improves cell-cell adhesion by increasing α-E-catenin in oral cancer cells". FEBS Lett. 586 (6): 924-33. doi: ...
B. C. Giovanella u. a.: Development of invasive tumors in the nude mouse after injection of cultured human melanoma cells. In: ... L. Miers u. a.: Implantation of different malignant human cell lines in an athymic mouse does not alter success and growth ... D. Kong u. a.: Establishment and characterization of human pancreatic adenocarcinoma cell line in tissue culture and the nude ... T. Devos u. a.: Occurrence of autoimmunity after xenothymus transplantation in T-cell-deficient mice depends on the thymus ...
... it is possible to localize their source along a straight line of coincidence (also called the line of response, or LOR). In ... For example, 11C-labelled metomidate (11C-metomidate), has been used to detect tumors of adrenocortical origin.[5][6] Also, ... This tracer is a glucose analog that is taken up by glucose-using cells and phosphorylated by hexokinase (whose mitochondrial ... Sweet, W.H.; G.L. Brownell (1953). "Localization of brain tumors with positron emitters". Nucleonics. 11: 40-45.. ...
In a patient with Cushing's disease, the tumor cells will be stimulated to release corticotropin and elevated plasma ... The first-line treatment of Cushing's disease is surgical resection of ACTH-secreting pituitary adenoma; this surgery involves ... the large tumor can compress adjacent structures.[citation needed] These tumors can compress the nerves that carry information ... Some tumors do not contain a discrete border between tumor and pituitary gland; therefore, careful sectioning through pituitary ...
The retinoids appear to influence the cell life cycle in the follicle lining. This helps prevent the accumulation of skin cells ... These gene candidates include certain variations in tumor necrosis factor-alpha (TNF-alpha), IL-1 alpha, and CYP1A1 genes, ... and Th1 cells.[45] IL-1α stimulates increased skin cell activity and reproduction, which, in turn, fuels comedo development.[45 ... and accumulation of skin cells in the hair follicle.[1] In healthy skin, the skin cells that have died come up to the surface ...
... has been known to stimulate cell growth in normal and cancer cell line cultures,[37] and it was shown that ... a potential target for novel medicines in malignant brain tumour therapies (mini-review)". Folia Neuropathologica. 45 (3): 99- ... In line with its role as a first line defense system, SP is released when toxicants or poisons come into contact with a range ... on cells (including cancer cells) bestowing upon them mobility.[40] and metastasis.[41] It has been suggested that cancer ...
Graft-versus-tumor effect[edit]. Main article: Graft-versus-tumor effect. Graft-versus-tumor effect (GVT) or "graft versus ... The mucosal lining of the bladder could also be involved in approximately 5 percent of the children undergoing hematopoietic ... a b Memorial Sloan-Kettering Cancer Center , Blood & Marrow Stem Cell Transplantation , The Graft-versus-Tumor Effect Archived ... who have lost their stem cells after birth. Other conditions[13] treated with stem cell transplants include sickle-cell disease ...
California Institute for Regenerative Medicine, Tumor Cells Become Drug Resistant by Reverting to a Stem Cell-Like State, New ... 19,0 19,1 P. De Coppi, G Barstch, Anthony Atala (2007). "Isolation of amniotic stem cell lines with potential for therapy". ... Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM (1998). "Embryonic stem cell lines ... Lindvall O (2003). "Stem cells for cell therapy in Parkinson's disease". Pharmacol Res 47 (4): 279-87. PMID 12644384. ...
Agonists: Glial cell line-derived neurotrophic factor (GDNF). *Liatermin. *Kinase inhibitors: Vandetanib ... Minkovsky N, Berezov A (December 2008). "BIBW-2992, a dual receptor tyrosine kinase inhibitor for the treatment of solid tumors ... It has received regulatory approval for use as a treatment for non-small cell lung cancer,[6][4][7][8] although there is ... Afatinib, sold under the brand name Gilotrif among others, is a medication used to treat non-small cell lung carcinoma (NSCLC). ...
A thyroglossal cyst is lined by pseudostratified, ciliated columnar epithelium while a thyroglossal fistula is lined by ... The removal of cells for biopsy, using a needle Clinical features[edit]. Clinical features can be found in the subhyoid portion ... These tumors usually arise from the ectopic thyroid tissue within the cyst.[6][7] ... Thyroglossal cysts can be defined as an irregular neck mass or a lump which develops from cells and tissues left over after the ...
... make it useful for making cell lines that can be used to test serotyping antibodies. As a result, HLA-A1 and B8 produce some of ... Most important of which is the TNF (tumor necrosis factors) with 3 loci in the region. Starting from B8, immediately followed ... In 1975, association with "HL-A1,8" (Current name: HLA A1-B8) was confirmed by serological typing of cells from myasthenics.[11 ... June 2004). "Genetic polymorphisms in tumor necrosis factor (TNF)-alpha and TNF-beta in a population-based study of systemic ...
... example of applying the use of retroviral replicating vectors towards transducing cell lines derived from solid tumors.[61] ... A brain tumor occurs when abnormal cells form within the brain.[2] There are two main types of tumors: malignant or cancerous ... Choroid plexus tumor, Dysembryoplastic neuroepithelial tumour, Ependymal tumor, Fibrillary astrocytoma, Giant-cell glioblastoma ... Less commonly, and seen usually in infants, are teratomas and atypical teratoid rhabdoid tumors.[65] Germ cell tumors, ...
Cell Biol. 42 (6): 813-27. doi:10.1016/j.biocel.2009.11.013. PMID 19931639.. ... Lang F, Alevizopoulos K, Stournaras C (2013). "Targeting membrane androgen receptors in tumors". Expert Opin. Ther. Targets. 17 ... Katsambas AD, Dessinioti C (2010). "Hormonal therapy for acne: why not as first line therapy? facts and controversies". Clin. ... Bennett NC, Gardiner RA, Hooper JD, Johnson DW, Gobe GC (2010). "Molecular cell biology of androgen receptor signalling". Int. ...
... or tumors such as lymphoma or squamous cell carcinoma. A tonsillolith (also known as a "tonsil stone") is material that ... The tonsils are immunocompetent organs which serve as the immune system's first line of defense against ingested or inhaled ... These M cells then alert the underlying B cells and T cells in the tonsil that a pathogen is present and an immune response is ... The tonsils have on their surface specialized antigen capture cells called M cells that allow for the uptake of antigens ...
"Defining the sister rat mammary tumor cell lines HH-16 cl.2/1 and HH-16.cl.4 as an in vitro cell model for Erbb2". PLOS ONE. 7 ... Cells, circulating tumor cells (CTCs), or formalin-fixed paraffin-embedded (FFPE) or frozen tissue sections are fixed, then ... in cells, circulating tumor cells, and tissue samples. In this context, it can help define the spatial-temporal patterns of ... FISH can also be used to detect diseased cells more easily than standard Cytogenetic methods, which require dividing cells and ...
Once the attention of the swarm is drawn to a certain line within the canvas, the capability of PSO is used to produce a ' ... the possibility of using swarm intelligence to control nanobots within the body for the purpose of killing cancer tumors.[23] ... swarmic sketch' of the attended line. The swarms move throughout the digital canvas in an attempt to satisfy their dynamic ... swarms embark on interpreting the input line drawings. In other works while PSO is responsible for the sketching process, SDS ...
This gene is a transcription factor that regulates the cell cycle and hence functions as a tumor suppressor. By inducing G ( ... the body's first line of defense to fight infections. In 2016, the molecular mechanism was uncovered as damage to the ... pyrene diol epoxide inactivates the tumor suppression ability in certain cells, leading to cancer. ... BaP was shown to cause genetic damage in lung cells that was identical to the damage observed in the DNA of most malignant lung ...
... cancer that begins in egg cells).. *Mucinous appendiceal carcinoma: A type of cancer that begins in cells that line the ... Mesothelioma: A benign (noncancerous) or malignant (cancerous) tumor affecting the lining of the chest or abdomen. Exposure to ... and mucus-secreting cells may attach to the peritoneal lining and continue to secrete mucus. ... Thymoma and Thymic carcinoma: These tumors which arise from the thymus gland in the upper part of the chest overlying the heart ...
"The consequences of Rad51 overexpression for normal and tumor cells". DNA Repair (Amst.). 7 (5): 686-93. doi:10.1016/j.dnarep. ... initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines". ... Esophageal squamous cell cancer. Over-expression. 47%. Immunohistochemistry. [24]. Renal cell carcinoma. Under-expression. 100% ... "Stable interaction between the products of the BRCA1 and BRCA2 tumor suppressor genes in mitotic and meiotic cells". Molecular ...
β-Glucans (beta-glucans) comprise a group of β-D-glucose polysaccharides naturally occurring in the cell walls of cereals, ... Sikora, Per (14 June 2012). "Identification of high b-glucan oat lines and localization and chemical characterization of their ... 3-glucans enhance the tumoricidal activity of antitumor monoclonal antibodies in murine tumor models". Journal of Immunology. ... One of the most common sources of β(1,3)D-glucan for supplement use is derived from the cell wall of baker's yeast ( ...
tumors, frequently solid tumors of the lung and colon; hematological malignancies such as chronic lymphocytic leukemia are less ... Recommended first line therapy often includes: cyclophosphamide alternating with a corticosteroid.[10] ... This, in turn, stimulates release of proteases and oxidants by the mesangial and epithelial cells, damaging the capillary walls ... and causing them to become "leaky". In addition, the epithelial cells also seem to secrete an unknown mediator that reduces ...
In line with the Hindu yoga tradition, Harrison became a vegetarian in the late 1960s.[354] After being given various religious ... it was reported that he was being treated for a brain tumour at a clinic in Switzerland.[196] While in Switzerland, Starr ... he began radiotherapy at Staten Island University Hospital in New York City for non-small cell lung cancer that had spread to ... Greene 2006, p. 69: In line with the Hindu yoga tradition; Clayson 2003, p. 208; Greene 2006, p. 158: Harrison became a ...
DNA from a rhabdomyosarcoma cell line and a fibrosarcoma cell line transformed a NIH/3T3 mouse fibroblast cell line. After ... injection into mice, tumors started to form in as little as 10 days. Next, the transforming activities of the rhabdomyosarcoma ... cells. Downregulation of RhoA in the HBE cell lines using siRNAs showed a lack of apical junction formation in contrast with ... Further DNA testing showed that the transforming sequences in the two cancer cell lines were the same, and the gene was later ...
... and intraocular tumors. It is also being used increasingly during surgery for brain tumors. ... In cellular biology, the isothiocyanate derivative of fluorescein is often used to label and track cells in fluorescence ... DyLight Fluor, a product line of fluorescent dyes. *Fluorescein diacetate hydrolysis, a biochemistry laboratory test ... allowing biologists to target the fluorophore to specific proteins or structures within cells. This application is common in ...
His interest in immunology has led to publications in HIV disease, cellular activation and natural killer cell function, tumor ... Additionally, Strauss has published on peripheral and central line catheters, anesthesia and surgical devices, safety injection ... The staging and prognostic value of subset markers on CD8 cells in HIV disease. In Janossy G, Autran B. Miedema F (eds): ... Enumeration of CD4+ T-cells in the peripheral blood of HIV-infected patients: interlaboratory study of the FACSCount system. ...
... cells - CDC National Prevention Information Network (CDC-NPIN) - cell lines - cell-mediated immunity (CMI) - cellular immunity ... tumor necrosis factor (TNF) ... T suppressor cells - T4 cell - T4 cells (T-helper cells) - T8 ... B-cell lymphoma - B cells - B lymphocytes (B cells) - bactericidal - bacteriostatic - bacterium - baculovirus - baseline - ... human T cell lymphotropic virus type I (HTLV-I) - human T cell lymphotropic virus type II (HTLV-II) - humoral immunity - HVTN ...
Natural killer cells: virus-infected and tumor cells.. Deeply staining, eccentric. NK-cells and cytotoxic (CD8+) T-cells. Years ... Leukocytosis may affect one or more cell lines and can be neutrophilic, eosinophilic, basophilic, monocytosis, or lymphocytosis ... T cells: *CD4+ helper T cells: T cells displaying co-receptor CD4 are known as CD4+ T cells. These cells have T-cell receptors ... CD8+ cytotoxic T cells: virus-infected and tumor cells.. *γδ T cells: bridge between innate and adaptive immune responses; ...
... cell responses to mitogens and allogeneic cells, cytokine production by cells Tests for B cell function: antibodies to routine ... Immune deficiencies can result in persistent or recurring infections, autoinflammatory disorders, tumors, and disorders of ... which is a basic line of defence that is independent of the more advanced lymphocyte-related systems. Many of these conditions ... natural killer cells and monocytes (CD15+), as well as activation markers (HLA-DR, CD25, CD80 (B cells). Tests for T cell ...
The first-line psychiatric treatment for schizophrenia is antipsychotic medication,[35] which can reduce the positive symptoms ... but clozapine can lower the white blood cell count in 1 to 4 percent of people who take it. This is a serious side effect.[16][ ... A stroke or brain tumor. *A metabolism (chemical reactions keeping the person alive) that is too high or too low ... using stem cells. This could help with schizophrenia and autism neurological research (research related to the brain.) [62] ...
Rous sarcoma virus contains the src gene that triggers tumor formation. Later it was found that a similar gene in cells is ... When retroviruses have integrated their own genome into the germ line, their genome is passed on to a following generation. ... the cell membrane degrades, becoming part of the host cell, and the RNA strands and enzymes enter the cell (3). Within the cell ... In this way some retroviruses can convert normal cells into cancer cells. Some provirus remains latent in the cell for a long ...
Wilms Tumour(tumor) cell lines. Mr. D.M. Shaw incubus at liverpool.ac.uk Thu Jun 13 09:11:29 EST 1996 *Previous message: A260 ... Hi cell biologists, I am looking for tumour cell lines derived from childhood renal tumours such as Wilms or the bone marrow ... metastasizing renal tumour of chilhood (BMRTC). Can anyone help? Please send replies vie E-mail incubus at liv.ac.uk Many ...
ATCC announces the addition of ATCC Tumor Cell Panels to its product offering for aid in cancer research, drug discovery, ... KRAS were assessed and tumor cell lines in the ATCC collection that carried those mutations were identified, with key cells ... ATCC announces the addition of ATCC Tumor Cell Panels to its product offering for aid in cancer research, drug discovery, ...
However, the T3 effect on cell proliferation appears to be dependent on the type of tumor cell line with aggressive tumors to ... whereas its effect on cell proliferation appears to be dependent on the type of tumor cell line with aggressive tumors being ... Cell-Type-Dependent Thyroid Hormone Effects on Glioma Tumor Cell Lines. Liappas Alexandros,1,2 Mourouzis Iordanis,1 Zisakis ... All cell lines were maintained in a 37°C humidified incubator with 5% CO2. For all experiments, each of the glioma cell lines ...
The US National Cancer Institute (NCI) 60 human tumour cell line anticancer drug screen (NCI60) was developed in the late 1980s ... The NCI60 human tumour cell line anticancer drug screen.. Shoemaker RH1. ... model was rapidly recognized as a rich source of information about the mechanisms of growth inhibition and tumour-cell kill. ...
Analysis of topoisomerase II immunoprecipitates from 32P-labeled HeLa cells indicated that phosphorylation of the enzyme ... The phosphorylation of the nuclear enzyme DNA topoisomerase II was characterized from HeLa human cervical carcinoma cells ... 1991). Phosphorylation of DNA topoisomerase II in a human tumor cell line. Journal of Biological Chemistry, 266(12), 7957-7961 ... The phosphorylation of the nuclear enzyme DNA topoisomerase II was characterized from HeLa human cervical carcinoma cells ...
In the present work we have screened a variety of different tumor cell lines for p185HER2 expression using both enzyme-linked ... Differential responses of human tumor cell lines to anti-p185HER2 monoclonal antibodies Cancer Immunol Immunother. 1993 Sep;37( ... In the present work we have screened a variety of different tumor cell lines for p185HER2 expression using both enzyme-linked ... was also tested in soft-agar growth assays for activity against p185HER2-overexpressing tumor cell lines of each type, with ...
... Fulya Üstün Alkan,1 Oya Üstüner,1 Tülay Bakırel,1 ... "Synergistic growth inhibitory effect of deracoxib with doxorubicin against a canine mammary tumor cell line, CMT-U27," Journal ... "Citrate and celecoxib induce apoptosis and decrease necrosis in synergistic manner in canine mammary tumor cells," Cellular and ... "In Vitro Effects Of Doxorubicin And Deracoxib On Oxidative-Stress-Related Parameters In Canine Mammary Carcinoma Cells," Acta ...
... can become any type of cell in the adult body, offering great potential in disease modeling, drug discovery and creating ... replacement cells for conditions ranging from cardiovascular to Alzheimers disease. ... Novel Stem Cell Line Avoids Risk of Introducing Transplanted Tumors Progenitor cells might eventually be used to repair or ... Home / Newsroom / Releases / Novel Stem Cell Line Avoids Risk of Introducing Transplanted Tumors ...
In women, germ cells form the eggs in the ovaries. In men, germ cells form the sperm in the testicles. ... Germ cells are the cells in your body that are used for reproduction. ... Germ cell tumors grow in the ovaries and testes. Germ cells are the cells in your body that are used for reproduction. In women ... How are germ cell tumors treated?. Germ cell tumors tend to respond well to treatments. Your doctor will work with you to ...
... researchers at Washington State University report a new approach for the effective capture of tumor-derived exosomes from a ... prostate cancer cell line. Exosomes are small secreted vesicles that play a key role in intercellular communication and cancer ... New approach for the capture of tumor-derived exosomes from a prostate cancer cell line Findings enable a non-invasive approach ... New approach for the capture of tumor-derived exosomes from a prostate cancer cell line. Springer ...
PubMed journal article Expression of selenium-dependent glutathione peroxidase in human breast tumor cell line were found in ... A near absence of hgpx1 mRNA expression was observed in 3 of 13 ER-negative cell lines; 1 of 4 ER-positive cell lines had high ... A near absence of hgpx1 mRNA expression was observed in 3 of 13 ER-negative cell lines; 1 of 4 ER-positive cell lines had high ... Here we report the results from a larger survey of breast cancer cell lines, including six recently isolated cell lines. ...
... in cervical cancer cells and in one line of leukemia, tumor cells. Other cell lines were resistant, including ovarian and ... Published online today in Cell Cycle, the researchers describe how four different human tumor cells lines out of 16 tested were ... Synthetic cocoa chemical slows growth of tumors in human cell lines. 17.06.2008 ... The strongest response was seen in two different colon cancers; growth was cut in half and most of the tumor cells were damaged ...
Resistant cell lines are indicated in black. Cell lines not screened with a particular compound are indicated in gray. ... Details regarding the most sensitive cell lines identified are shown in the chart, and the cell lines are shown in order of ... The tumor type is also indicated. For comparison, the sorafenib table also shows the sensitivity of cell lines to 200 and 2 μM ... Profiling 500 tumor cell lines with a variety of selective kinase inhibitors reveals a wide range of sensitivities for most ...
... were produced by immunizing mice with the GD2-expressing neuroblastoma cell line LAN-1 and a prefusion boost with purified GD2 ... Disialoganglioside GD2 in human neuroectodermal tumor cell lines and gliomas Acta Neuropathol. 1991;82(1):45-54. doi: 10.1007/ ... tumor cells in 24 of 30 (80%) cases stained positively with DMAb-20. Reactive astrocytes, both within the adjacent to tumors, ... DMAb-20 was used to evaluate the expression of GD2 by malignant glioma and medulloblastoma cell lines using cell surface ...
... by which some genetically deregulated and aggressive tumour cells generat ... Vascuologenesis is the de novo establishment of blood vessels and vascular networks from mesoderm-derived endothelial cell ... cell lines and other aggressive tumour cell lines. We present experimental evidence of vasculogenic mimicry in HNSCC cell lines ... Metastatic HNSCC cells lines were found to have vasculogenic properties similar to HUVEC cell lines when compared to cell lines ...
Cell cycle arrest at G2/M phase was found after SB treatment. UOK146 cell line shows autophagy mode of cell death as displayed ... Sodium butyrate induces cell death by autophagy and reactivates a tumor suppressor gene DIRAS1 in renal cell carcinoma cell ... translocated renal cell carcinoma cell line UOK146 was studied. Anti-proliferative effect of SB in renal cell carcinoma (RCC) ... Verma SP, Tripathi VC, Das P (2014) Asparagus racemosus leaf extract inhibits growth of UOK 146 renal cell carcinoma cell line ...
Following transfection, stable clones in two EC cell lines were selected. The effects of HE4 overexpression on cell growth and ... HE4-induced cancer cell proliferation in vivo was examined in a mouse xenograft model. HE4 overexpression significantly ... HE4 is also overexpressed in endometrial cancer (EC), but its function in cancer cells is not clear. In this study, we ... HE4 overexpression enhanced several malignant phenotypes in cell culture and in a mouse model. These results are consistent ...
Locale about Experts and Doctors on tumor cell line in Houston, United States ... Responses in mantle cell lymphoma cells to SNS-032 depend on the biological context of each cell line. Cancer Res. 2010;70:6587 ... You are here: Locale , United States , Texas , Experts and Doctors on tumor cell line in Houston, United States ... Experts and Doctors on tumor cell line in Houston, United States. Summary. Locale: Houston, United States ...
Top : New Forum Archives (2009-): : Cell Biology. p53 +/- matched-pair tumor cell lines? - (Dec/06/2009 ). Hi,. I ll try to ... Is there any matched-pair cell line available so we can test our compounds on p53 positive tumor cells and p53 mutated/Null ... Theres some HCT116 cells around that are isogenic (I think), though they do contain some of the isoforms of p53, so p53 may ... Hopefully these compounds will have an antiproliferative activity against p53 Null cells. ...
P-selectin Expression in a Metastatic Pancreatic Tumor Cell Line (SUIT-2). Takeshi Iwamura, Thomas C. Caffrey, Norio Kitamura, ... endothelial cells, and these pancreatic tumor cells. The finding that P-selectin is expressed by metastatic pancreatic tumor ... The human pancreatic tumor cell line SUIT-2 was derived from a metastatic lesion in the liver of a patient with pancreatic ... P-selectin Expression in a Metastatic Pancreatic Tumor Cell Line (SUIT-2) ...
Autologous, proliferating, self-renewing tumor cells (cancer stem cells... ... or autologous dendritic cells (DCV), loaded with antigens from their tumor cell line. Cell lines were successfully established ... self-renewing tumor cells (cancer stem cells and/or early progenitor cells), which are responsible for metastatic tumors, could ... Cancer Stem Cell Antigens from Autologous Tumor Cell Lines in Patient-Specific Active Immunotherapy for Metastatic Cancer. ...
Identification of tumor-initiating cells derived from two canine rhabdomyosarcoma cell lines * * KISHIMOTO Takuya Evan ... p,Cancer stem cells or tumor-initiating cells (TICs) are a small subpopulation of cells that have the capacity to self-renew, ... differentiate and initiate tumors. These cells may function in tumor initiation, aggression and recurrence. Whether spheres ... We induced sphere formation in the canine rhabdomyosarcoma cell lines, CMS-C and CMS-J, and characterized the spheres ,i,in ...
Premium Human Tumor Total RNAs are high-quality RNA samples purified using a modified guanidinium thiocyanate method. ... Total RNA from human tumors and human cell lines. ... Human Stem Cell Derived Beta Cells * Human Stem Cell Derived ... We offer an extensive selection of total RNA from human tumors and human cancer cell lines. ... Total RNA, Human Cancer & Cell Line. Total RNA from Clontech is meticulously prepared to high quality using our proprietary ...
Effects of TKIs on sarcoma cell line proliferation. Which, if any, of the roughly 40 TKs we identified in tumor cell lines act ... A, phosphotyrosine expression in cell lines and human tumor tissues. Lysates from cells and tumor tissues were subjected to SDS ... Cell lines. Cell lines A204 (HTB-82), Saos2 (HTB-85), MG63 (CRL-1427), MNNG/HOS Cl#5 (CRL-1547), A673 (CRL-1598), RD-ES(HTB-166 ... A204 cell line dependent on PDGFRα signaling. A, signaling changed by TKIs in A204 cells. A204 cells were exposed to DMSO, ...
Buy or Rent Atlas of Human Tumor Cell Lines as an eTextbook and get instant access. With VitalSource, you can save up to 80% ... Atlas of Human Tumor Cell Lines Edition by Robert K.M. Hay and Publisher Academic Press. Save up to 80% by choosing the ...
Inhibitory Effects of Cholesterol Sulfate on Progesterone Production in Human Granulosa-like Tumor Cell Line, KGN * * TSUTSUMI ... Establishment and characterization of a steroidogenic human granulosa-like tumor cell line, KGN, that expresses functional ... Cholesterol sulfate (CS) is a component of cell membranes that plays a role in stabilizing the cell membrane. We previously ... derived from human granulosa-like tumor. KGN cells were cultured with CS (10 μM) or cholesterol (10 μM) in the presence of 8- ...
... using cell suspensions of 22 cloned and 8 subcloned cell lines derived from RINm as well as 11 other continuous cell lines ... Cytosolic Insulin-degrading Activity in Islet-derived Tumor Cell Lines and in Normal Rat Islets. ... Cytosolic Insulin-degrading Activity in Islet-derived Tumor Cell Lines and in Normal Rat Islets ... Cytosolic Insulin-degrading Activity in Islet-derived Tumor Cell Lines and in Normal Rat Islets ...
6-Aminonicotinamide sensitizes human tumor cell lines to cisplatin.. I I Budihardjo, D L Walker, P A Svingen, C A Buckwalter, S ... 6-Aminonicotinamide sensitizes human tumor cell lines to cisplatin.. I I Budihardjo, D L Walker, P A Svingen, C A Buckwalter, S ... 6-Aminonicotinamide sensitizes human tumor cell lines to cisplatin.. I I Budihardjo, D L Walker, P A Svingen, C A Buckwalter, S ... 6-Aminonicotinamide sensitizes human tumor cell lines to cisplatin. Message Subject (Your Name) has forwarded a page to you ...
Human Tumor Cell Line: A431, 0.1 mg. Tissue total protein is prepared from whole tissue homogenates and presents a consistent ... Human Tissue Tumor Cell line A431, Human Tumor Cell line A431, Human Tumor Cell line A431 Lysate, Human Tumor Cell line A431 ... Human protein lysate Tumor Cell line A431, Protein from Human, Tissue from Human, Tumor Cell line lysate, Tumor Cell line ... NB820-89386 Human Tumor Cell Line: A431 Search for all "Human Tumor Cell Line: A431" ...
Safety Study of DIPG Treatment With Autologous Dendritic Cells Pulsed With Lysated Allegenic Tumor Lines. The safety and ... treatment with autologous dendritic cells pulsed with lysated allegenic tumor lines. Evaluate the nonspecific immune response ... Neuroectodermal Tumors. Neoplasms, Germ Cell and Embryonal. Neoplasms by Histologic Type. Neoplasms. Neoplasms, Glandular and ... Ib Clinical Trial on the Safety of Immunotherapy With Autologous Dendritic Cells Primed With Lysate Allogeneic Tumor Lines in ...
  • Through use of the COSMIC database, 20 genes associated with tumorigenesis including TP5E, CDKN2A, BRAF and KRAS were assessed and tumor cell lines in the ATCC collection that carried those mutations were identified, with key cells lines parceled into easy- to-order, cost-effective panels. (drugdiscoverynews.com)
  • These data suggest that crosstalk mechanisms exist between the 15-LOX-2 gene and PPARgamma to counterbalance expression and help explain the inverse relationship of these genes in normal versus cancer cells. (labome.org)
  • In the present study, we investigated the effect of CS on the expression of progesterone production-related genes in KGN cells, derived from human granulosa-like tumor. (nii.ac.jp)
  • AQPs 1, 3, 4, 5, 8 and 9 were screened in the NSCLC cell line H1299, and the present results showed that AQP5 mRNA was upregulated compared with the other AQP genes. (spandidos-publications.com)
  • Moreover, we studied the transcriptional targets in these cells in response to cisplatin, particularly the expression profiles of genes associated with DNA damage response. (uio.no)
  • When rat 6 cells were cotransfected with pT24 and neo genes and grown in the absence or presence of TPA, the presence of TPA did not increase the yield of Neo+ colonies but caused a fivefold increase in the number of Neo+ colonies that displayed a transformed morphology. (asm.org)
  • In vivo genomic footprinting of thyroid hormone-responsive genes in pituitary tumor cell lines. (asm.org)
  • For this purpose, the introduction of genes that confer tumors with bioluminescent properties has been a critical advance for oncologic studies in rodents. (bio-protocol.org)
  • Methods of introducing bioluminescent genes, such as firefly luciferase, by viral transduction has allowed for the production of tumor cell lines that can be followed in vivo longitudinally over long periods of time. (bio-protocol.org)
  • Lower methylation of CpNpG sequences characteristic of all transformed cells could result from the disturbance of one of several plant DNA methyltransferase genes following its homologous recombination with the M·EcoRII gene. (deepdyve.com)
  • Activation of tumor suppressor genes in nontumorigenic revertants of the HeLa cervical carcinoma cell line -- Boylan et al. (aacrjournals.org)
  • Cell fusion experiments indicated that the revertant phenotypes of HA and HF cells resulted from mutations in cellular genes that activate one or more tumor suppressor genes. (aacrjournals.org)
  • MicroRNAs (miRNAs) regulate cell proliferation, differentiation, and apoptosis functioning as tumor suppressor genes but their role in NET proliferation and metastasis has not been fully evaluated. (enets.org)
  • Since the early stages of tumorigenesis involve adhesion, escape from immune surveillance, vascularization and angiogenesis, we devised a strategy to study the expression profiles of all publicly known and putative secreted and cell surface genes. (biomedcentral.com)
  • We designed a custom oligonucleotide microarray containing probes for 3531 secreted and cell surface genes to study 5 diverse human transformed cell lines and their derivative xenograft tumors. (biomedcentral.com)
  • 05) to tumor data revealed a larger set of 149 differentially expressed genes. (biomedcentral.com)
  • 3) After MANOVA was performed on data from individual tumors, a comparison of differential genes amongst all tumor types revealed 12 common differential genes. (biomedcentral.com)
  • In this study, we examined this problem with a more focused approach with respect to the transcripts as well as a broader survey by examining multiple tumor sources in order to identify differential genes common to multiple solid tumors in a xenograft model of tumorigenesis. (biomedcentral.com)
  • Since the end-point of the xenograft assay is the formation of a solid tumor, genes supporting vasculogenesis and angiogenesis are likely differentially expressed relative to the parental cell lines that were adapted to culture in vitro. (biomedcentral.com)
  • Aberrant promoter methylation of tumor suppressor genes has not been fully investigated in pediatric tumors. (nih.gov)
  • Therefore, we examined the methylation status of nine genes (p16(INK4A), MGMT, GSTP1, RASSF1A, APC, DAPK, RARbeta, CDH1 and CDH13) in 175 primary pediatric tumors and 23 tumor cell lines using methylation-specific PCR. (nih.gov)
  • High rates of loss of heterozygosity commonly affect multiple chromosomes in individual tumor types, yet the number of known tumor suppressor genes (TSGs) systematically mutated in the corresponding tumors is usually low. (pasteur.fr)
  • Evolution of the initial tumour cell may occur by two methods: Sequentially ordered mutations accumulate in driver genes, tumour suppressor genes, and DNA repair enzymes, resulting in clonal expansion of tumour cells. (wikipedia.org)
  • The US National Cancer Institute (NCI) 60 human tumour cell line anticancer drug screen (NCI60) was developed in the late 1980s as an in vitro drug-discovery tool intended to supplant the use of transplantable animal tumours in anticancer drug screening. (nih.gov)
  • Willert, with co-corresponding author David Brafman, PhD, at Arizona State University, and colleagues engineered an in vitro microenvironment that permitted homogenous expansion of hPSC progenitor cells from the mesoderm - one of the three primary germ layers in early embryonic development. (ucsd.edu)
  • DMAb-20 will be useful in determining the functional role of GD2 in cell-cell interaction, adhesion, and invasion, and in defining altered growth control mechanisms of central nervous system neoplasms in in vitro models. (nih.gov)
  • Dillman RO, Nayak SK, Beutel L (1993) Establishing in vitro cultures of autologous tumor cells for use in active specific immunotherapy. (springer.com)
  • cDDP-resistant cells (T7800R) had significantly higher MT mRNA and MT protein, increased resistance to killing by cDDP and altered in vitro growth kinetics compared to parental T7800 cells. (aspetjournals.org)
  • These data indicate that selection in vitro for cDDP resistance in human germ cell tumors coselects for cells with enhanced MT content. (aspetjournals.org)
  • Therefore, the aim of the present systematic review was to summarize the available literature on the in vitro anti‑tumor effects of metformin on head and neck squamous cell carcinoma (HNSCC). (spandidos-publications.com)
  • Only in vitro studies analyzing the effects of metformin on HNSCC cell lines were included. (spandidos-publications.com)
  • These studies demonstrated that metformin is important in inhibiting cell proliferation, inducing G0/G1 cell cycle arrest and apoptosis, and in regulating proteins involved in carcinogenesis pathways, which corroborates its potential in vitro anti‑tumor effects. (spandidos-publications.com)
  • Sapphyrins have been explored as potential photodynamic therapy agents to inactivate viruses in vitro and also to treat superficial squamous cell carcinoma in mice ( 5 , 6 ). (aacrjournals.org)
  • Taken together, the data indicate that flavopiridol has activity against glioma cell lines in vitro and should be considered for clinical development in the treatment of glioblastoma multiforme. (aacrjournals.org)
  • It induces growth arrest at either the G 1 and/or G 2 phases of the cell cycle in numerous cell lines in vitro by acting as a competitive binding agent for the ATP-binding pocket of CDK ( 18 ). (aacrjournals.org)
  • HBLAK may be valuable for evaluating the tumor specificity of novel cancer drugs, but may also be applied as an urothelial in vitro carcinogenesis model. (iospress.com)
  • Tu-2449 thus displays growth characteristics and patterns of resistance to apoptosis similar to those of other mouse and human glioma cell lines and may therefore become a valuable tool to evaluate new therapies for malignant gliomas in vitro and in vivo. (uzh.ch)
  • Re-expression of the miR-200b/200a/429 cluster in RJ423 cells significantly suppressed the expression of Vim, Snai1, Twist1, Twist2 and Zeb1, reverted RJ423 cells to a more epithelial morphology and significantly inhibited proliferation in vitro. (ovid.com)
  • 1990 ). Tumor necrosis factor-alpha augments pulmonary arterial transendothelial albumin flux in vitro. (biologists.org)
  • We thus decided to investigate the effects in vitro of STS in combination with radiotherapy in metastatic cancer cells and non-cancer cells. (physiciansweekly.com)
  • In summary, IPH-926 significantly extends the biological spectrum of the established breast cancer cell lines and will facilitate functional analyses of genuine human ILBC cells in vitro and in vivo. (ovid.com)
  • In vitro human prostate cell culture models are critical for clarifying the mechanism of prostate cancer progression and for testing preventive and therapeutic agents. (elsevier.com)
  • Metastatic HNSCC cells lines were found to have vasculogenic properties similar to HUVEC cell lines when compared to cell lines from their corresponding primary tumour. (biomedsearch.com)
  • The human pancreatic tumor cell line SUIT-2 was derived from a metastatic lesion in the liver of a patient with pancreatic adenocarcinoma. (aacrjournals.org)
  • The finding that P-selectin is expressed by metastatic pancreatic tumor cells demonstrates that the range of cell types that express these adhesion molecules is broader than believed previously. (aacrjournals.org)
  • Autologous, proliferating, self-renewing tumor cells (cancer stem cells and/or early progenitor cells), which are responsible for metastatic tumors, could be excellent sources of antigen for vaccines that could be used for the active specific immunotherapy of patients with advanced cancer. (springer.com)
  • Dillman RO, Beutel LD, Barth NM, de Leon C, O'Connor AA, DePriest C, Nayak SK (2002) Irradiated cells from autologous tumor cell lines as patient-specific vaccine therapy in 125 patients with metastatic cancer: induction of delayed-type hypersensitivity to autologous tumor is associated with improved survival. (springer.com)
  • Skeletal metastatic disease is a deleterious consequence of dissemination of tumor cells from numerous primary sites, such as prostate, lung and breast. (diva-portal.org)
  • Whether cancer exosomes are involved in tumor and bone cell interactions in the metastatic site is still, however, a rather unexplored field. (diva-portal.org)
  • Our findings suggest that exosomes released from tumor cells in the tumor-bone interface are involved in pathological regulation of bone cell formation in the metastatic site. (diva-portal.org)
  • Circulating tumor cell counts could be used to help clinicians choose between first-line hormone therapy (the recommendation option) or chemotherapy for patients with ER-positive, HER2-negative metastatic breast cancer. (ascopost.com)
  • A phase III study by Bidard et al investigated whether circulating tumor cells could help physicians choose between hormone therapy or chemotherapy as front-line therapy for patients with estrogen receptor (ER)-positive, HER2-negative metastatic breast cancer. (ascopost.com)
  • Effects of radiotherapy and short-term starvation combination on metastatic and non-tumor cell lines. (physiciansweekly.com)
  • STS followed by single high-dose radiation significantly increased DNA damage in metastatic cancer cell lines but not in normal cells. (physiciansweekly.com)
  • Furthermore, STS reduced the surviving fraction of irradiated tumor cells, indicating a good radio-sensitizing effect on metastatic cell lines. (physiciansweekly.com)
  • We report the establishment of a permanent ILBC cell line, named IPH-926, which was derived from a patient with metastatic ILBC. (ovid.com)
  • To investigate the molecular mechanisms by which carcinoma cells metastasize, we have established liver metastatic models. (nii.ac.jp)
  • After from 6 to 8 weeks, the liver and regional lymph nodes with a few metastatic foci of AZ521 cells were dissected, we established AZH1G and AZL5G. (nii.ac.jp)
  • 2) Each metastatic cell grew significantly larger than parental AZ521 in vivo. (nii.ac.jp)
  • 3) A cell motility assay demonstrated that the migration ability of each metastatic cell was clearly higher than that of AZ521. (nii.ac.jp)
  • Moreover, Comprehensive analysis of the biological behavior of highly metastatic cell lines and the less metastatic same parental line were demonstrated the differences in adhesive activity and VEGF production. (nii.ac.jp)
  • Neuroendocrine tumors (NET), especially well-differentiated endocrine tumors, are often slow-growing, indolent, and may not become clinically apparent until the manifestations of metastatic spread or carcinoid syndrome. (enets.org)
  • To recapitulate the attachment and growth of a micro- or metastatic tumor, our experimental tumorigenesis model examined human xenograft tumors in nude mice. (biomedcentral.com)
  • In this study we have explored the effect of glucocorticoids on the migration of the ER-negative MDA-MB-231 human breast tumour cell line and the highly metastatic MDA-MB-231-HM.LNm5 cell line that was generated through in vivo cycling. (qub.ac.uk)
  • Selection of cells for high metastatic potential resulted in a less migratory cell phenotype that was resistant to regulation by glucocorticoids and showed decreased GR receptor expression. (qub.ac.uk)
  • These findings highlight the highly plastic nature of tumour cells, and underscore the need to more fully understand the direct effect of glucocorticoid treatment on different stages of metastatic progression. (qub.ac.uk)
  • Metastatic capacity of these treated cell lines was also depleted. (oncotarget.com)
  • Tumour heterogeneity describes the observation that different tumour cells can show distinct morphological and phenotypic profiles, including cellular morphology, gene expression, metabolism, motility, proliferation, and metastatic potential. (wikipedia.org)
  • Solid tumors, such as melanoma, expressing high levels of dGK should be considered for nucleoside analog therapy preferably in combination with their standard treatment. (diva-portal.org)
  • More recently some of the newer analogs have been used successfully to treat solid tumors as well. (diva-portal.org)
  • We are pursuing similar vaccination approaches for solid tumors. (leidenbiosciencepark.nl)
  • The molecular mechanisms underlying the sensitivity of testicular germ cell tumor cell lines to cisplatin treatment seem to be multifactorial, possibly including a low capacity to remove cisplatin-DNA adducts by nucleotide excision repair (NER), and their proneness to die by apoptosis. (uio.no)
  • Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is considered to induce apoptosis in a variety of cancer cells but not normal cells. (unboundmedicine.com)
  • In the present study, we examined whether chemotherapeutic agents enhance TRAIL-induced apoptosis in the sarcoma cell lines MG-63 and SaOS-2. (unboundmedicine.com)
  • Pretreatment with sub-toxic or slightly toxic concentrations of chemotherapeutic agents (cis-diammine dichloroplatinum, CDDP and doxorubicin, DXR) sensitized both cell lines to TRAIL-induced apoptosis, as assessed by the propidium iodide or Annexin V-Cy5 staining method. (unboundmedicine.com)
  • The CDDP-pretreated cells indeed demonstrated more increased TRAIL-mediated caspase-8 activation, loss of mitochondrial membrane potential (DeltaPsi(m)), and apoptosis than untreated cells. (unboundmedicine.com)
  • It was found that a dihydroxylated water-soluble sapphyrin derivative (PCI-2000) is a potent inducer of apoptosis in a wide variety of tumor cell lines including lymphoma (Ramos, DHL-4, and HF-1), leukemia (Jurkat and HL-60), and myeloma (8226/S, 1-310, C2E3, and 1-414). (aacrjournals.org)
  • Apoptosis can be partially inhibited by overexpression of the antiapoptotic protein Bcl-2 or treatment with benzyloxycarbonyl-valine-alanine-aspartic acid-fluoromethylketone, a cell-permeable caspase inhibitor. (aacrjournals.org)
  • In the present study, we show that water-soluble sapphyrins, such as the bis-hydroxypropyl derivative PCI-2000, can act as potent inducers of apoptosis in a wide variety of hematopoietic tumor-derived cell lines including lymphoma, leukemia, and myeloma. (aacrjournals.org)
  • Independent of retinoblastoma and p53 tumor suppressor pathway alterations, flavopiridol induced apoptosis in all cell lines but through a caspase-independent mechanism. (aacrjournals.org)
  • Tu-2449 and SRB-10 cells that showed low CD95 expression were resistant to CD95 ligand (CD95L)-induced apoptosis unless coexposed to CD95L and inhibitors of RNA or protein synthesis. (uzh.ch)
  • Inhibition of cellular proliferation and induction of apoptosis were investigated in three human lymphoid Leukaemia cell lines. (omicsonline.org)
  • Jurkat cells showed a significant synergistic induction of apoptosis following joint treatment with Falcarinol and a Death Receptor 5 agonist (DR5), whereas CCRF-CEM cells showed only an additive response. (omicsonline.org)
  • Conversely within MOLT-3 cells Falcarinol partially inhibited the induction of apoptosis by DR5 agonist although this failed to reach significance. (omicsonline.org)
  • However MOLT-3 cells demonstrated synergistic induction of apoptosis when Falcarinol was combined with either Bortezomib (proteosome inhibitor), or Sulforaphane (histone deacetylase inhibitor). (omicsonline.org)
  • 2000. Induction of apoptosis by hydrolysable tannins from Eugenia jambos L. on human leukemia cells. (dbpia.co.kr)
  • Loss of chromosome 6q21-qter is the second most frequent loss of chromosomal material in sporadic breast neoplasms suggesting the presence of at least one tumor suppressor gene on 6q, We recently isolated a cDNA encoding a new Zinc finger protein which we named ZAC according to its functional properties, namely induction of apoptosis and control of cell cycle progression. (cnrs.fr)
  • Thyroid hormone action on cell growth, differentiation and survival during development may be of therapeutic relevance Methods and Results 1321N1 cell line, an astrocytoma grade II, and U87MG, a glioblastoma grade IV, were exposed for 2 and 4 days in medium deprived of T3 and in medium containing 1 nM T3. (hindawi.com)
  • This screening model was rapidly recognized as a rich source of information about the mechanisms of growth inhibition and tumour-cell kill. (nih.gov)
  • Some monoclonal antibodies directed against the extracellular domain of p185HER2 inhibited growth in monolayer culture of breast and ovarian tumor cell lines overexpressing p185HER2, but had no effect on the growth of colon or gastric adenocarcinomas expressing increased levels of this receptor. (nih.gov)
  • The most potent growth-inhibitory anti-p185HER2 monoclonal antibody in monolayer culture, designated mumAb 4D5 (a murine IgG1 kappa antibody), was also tested in soft-agar growth assays for activity against p185HER2-overexpressing tumor cell lines of each type, with similar results. (nih.gov)
  • First, cultures derived from the latter often harbor undifferentiated cells that retain the potential to seed tumor growth. (ucsd.edu)
  • A synthetic chemical based on a compound found in cocoa beans slowed growth and accelerated destruction of human tumors in laboratory studies, and should be tested further for cancer chemoprevention or even treatment, say researchers at Georgetown University Medical Center. (innovations-report.com)
  • growth was cut in half and most of the tumor cells were damaged. (innovations-report.com)
  • The researchers do not yet clearly understand the mechanism by which GECGC disrupts tumor growth, but they think it inhibits the physical connections between cancer cells and blocks internal cell signaling pathways. (innovations-report.com)
  • RESULTS: There were observable differences in growth of the cells on laboratory plastic and collagen matrix. (biomedsearch.com)
  • The endothelial growth factor antibodies used did not inhibit or stimulate cell growth when compared to control but did discourage vascular mimicry. (biomedsearch.com)
  • This may explain its cell growth modulatory, anticarcinogenic, and radiosensitizing effects previously described. (labome.org)
  • Four of 10 cell lines showed dependence on tyrosine kinases for growth and/or survival, including platelet-derived growth factor receptor (PDGFR)α, MET, insulin receptor/insulin-like growth factor receptor signaling, and SRC family kinase signaling. (aacrjournals.org)
  • These include not enriching for patients whose tumor depends on the particular tyrosine kinase for growth/survival and a lack of assays that detect an activated tyrosine kinase that predicts drug sensitivity. (aacrjournals.org)
  • One technique that may be helpful to identifying tumor cells dependent on kinases for growth and/or survival, as well as charting the landscape of activated tyrosine kinases in tumor cells, is mass spectrometry (MS)-based phosphoproteomics ( 7 ). (aacrjournals.org)
  • NCI human tumor cell line growth inhibition assay. (nih.gov)
  • Similarly, vascular endothelial growth factor was significantly increased in control cells (si‑NC) compared with cells transfected with small interfering RNA targeting AQP5. (spandidos-publications.com)
  • They had a slower growth rate and, although the rank order of MT level in T7800, T7800R and other human tumor cell lines correlated very well with cDDP resistance, differences in the level of MT expression did not correspond with differences in the absolute level of cDDP resistance. (aspetjournals.org)
  • In addition, the cell models exhibit predictable growth rates and stable proteomic expression. (technologynetworks.com)
  • AMSBIO's extensive quality control process ensures stable growth rate for each lot of Cellaria cell models delivered. (technologynetworks.com)
  • PCI-2010 showed less toxicity in mice than PCI-2000 and was active in slowing the growth of Ramos and HL-60 tumor xenografts in nude mice. (aacrjournals.org)
  • Accordingly, PCI-2010 activity was studied in vivo and it was found to inhibit the growth of Ramos and HL-60 cells in mouse xenograft models. (aacrjournals.org)
  • When culturing TIC‐high cells, ensure that DMEM/F‐12, trypsin and growth factors cocktail mix are prewarmed in a 37°C water bath. (currentprotocols.com)
  • BACKGROUND: The role of estrogen in the growth and survival of ovarian cancer cells is controversial. (biomedsearch.com)
  • RESULTS: Estrogen 0.01-1.0μM inhibited the growth of both cell lines. (biomedsearch.com)
  • We examined an endogenous gene the growth hormone (GH) gene, and a stably transfected plasmid containing the chicken lysozyme silencer (F2) T3 response element (TRE) gene, F2-TRE-TK-CAT, both in pituitary tumor (GC) cells. (asm.org)
  • Therefore, re-expression of one or more miR-200 family members in mammary tumor cells with mesenchymal characteristics may restore an epithelial phenotype including growth and metastasis suppression. (ovid.com)
  • The transformed lines differed in their morphology, growth dependence on hormones, and nopaline-synthesizing capacity. (deepdyve.com)
  • 1963. Effects of sulphated degraded laminarin on experimental tumor growth. (dbpia.co.kr)
  • Cell Growth Differ. (aacrjournals.org)
  • Insulin-like growth factor-1 receptor (IGF-1R) was reported to be expressed in NET (neuroendocrine tumor) cells but its biological roles and activation status in non-functioning NET are unknown. (enets.org)
  • DCprime reported reduced tumour growth in both A375 and U87-MG tumour models following vaccination with KLH and tumour marking compared to the mice injected with PBS. (leidenbiosciencepark.nl)
  • Our previous studies demonstrated that, in other cell types, galangin is a potent inhibitor of the aryl hydrocarbon receptor (AhR), an environmental carcinogen-responsive transcription factor implicated in mammary tumor initiation and growth control. (biomedcentral.com)
  • Galangin inhibited transition of cells from the G 0 /G 1 to the S phases of cell growth, likely through the nearly total elimination of cyclin D3. (biomedcentral.com)
  • These results show that the cytotoxicity of retinoic acids and the growth promoting/inhibiting ability of the conditioned media is retinoic acid isoform, time, concentration, and cell type dependent. (usu.edu)
  • Moreover, physalin A significantly suppressed tumor xenograft growth. (oncotarget.com)
  • According to this hypothesis, tumours would be complex cell-subsets, even nameable as tissues, with the growth capacity and disorganized framework of the tumour being triggered by a small subset of tumour pathological cells exhibiting the characteristics of stem cells. (oncotarget.com)
  • Targeted inhibition of histone deacetylase leads to suppression of Ewing sarcoma tumor growth through an unappreciated EWS-FLI1/HDAC3/HSP90 signaling axis. (childrensmercy.org)
  • When proposed, this model allowed for the understanding of tumour growth, treatment failure, and tumour aggression that occurs during the natural process of tumour formation. (wikipedia.org)
  • The stability of topoisomerase II protein and topoisomerase II phosphorylation was also investigated in HeLa cells. (rti.org)
  • B ) Hierarchical clustering of the sensitivity of 500 human cancer cell lines to a single concentration of each of the 14 protein kinase inhibitors indicated in A . The concentration selected for each compound was that which most clearly discriminated between sensitive and resistant cell lines. (nih.gov)
  • SUIT-2 and clonal cell lines derived from it show spontaneous metastasis to lung and regional lymph nodes from s.c. nude mouse xenografts and were found to express P-selectin mRNA and protein. (aacrjournals.org)
  • Surface expression of P-selectin protein was increased by exposure of the pancreatic tumor cells to thrombin, oxygen radicals, and trypsin, suggesting that common cellular mechanisms for regulating P-selectin surface expression exist among platelets, endothelial cells, and these pancreatic tumor cells. (aacrjournals.org)
  • Whole cell lysates were extracted for western blot analysis with antibody for StAR protein. (nii.ac.jp)
  • At the protein level, AQP5 was significantly increased in H1299 cells compared with 16HBE cells. (spandidos-publications.com)
  • We found that dGK mRNA and protein expression was considerably higher in melanoma cells than in a leukemic cell line, while the difference at the activity level was less profound. (diva-portal.org)
  • Mini‐protein Tetra Cell for ready gel (Bio Rad, cat. (currentprotocols.com)
  • Given that MDM2 protein can confer oncogenic properties under certain circumstances, loss of MDM2 expression in tumor cells could promote increased chemosensitivity. (aacrjournals.org)
  • The cell lines express either wild-type p53 (A172, CCF-STTG1, and U87MG) or mutant p53 (T98G, U118MG, and U251MG), whereas one of six cell lines overexpresses MDM2 protein (CCF-STTG1), which acts to disrupt the p53 cell cycle pathway ( Fig. 1 Refs. (aacrjournals.org)
  • Only Tu-2449 cells accumulated p53 protein in response to genotoxic stress. (uzh.ch)
  • 1982 gamma MSH, a putative hormone in the N-terminal region of the ACTH/beta-endorphin (beta-EP) precursor protein, was studied by RIA with an antiserum against gamma 3MSH in ACTH-producing mouse pituitary tumor cells, AtT-20/D16v. (eurekamag.com)
  • Cell killing, DNA-interstrand crosslinks, and DNA-protein crosslinks were assayed in nitrogen mustard-resistant Walker 256 carcinoma (WR) cells and the parent cell line (WS) after treatment with 5-[3-(2-chloroethyl)-1-triazenyl]imidazo-4-carboxamide (MCTIC). (aspetjournals.org)
  • Following treatment with 100 microM MCTIC, there was a rapid accumulation of both DNA-interstrand and DNA-protein crosslinks in the WR cell line, which reached a maximum at 6 and 12 hr, respectively. (aspetjournals.org)
  • Protein levels of phospho-(p) and total AKT, ERK and p70S6K were assessed by Western blotting in MPC cells. (enets.org)
  • All mice - treatment arm and control group - received intraperitoneal vaccination with the KLH protein while the tumour marking in the treatment population was done by injecting DCOne mDC cells loaded with KLH protein directly into the tumour. (leidenbiosciencepark.nl)
  • By immunoprecipitation, two int-1 protein species, of 42 and 40 kD were identified in polygonal and in sphere-forming-cells but not in the culture media. (stanford.edu)
  • Membrane Protein _ Human Tumor Cell Line Raji Human samples 80 % of the research is conducted on human samples. (antibody-antibodies.com)
  • GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Membrane Protein _ Human Tumor Cell Line Raji. (antibody-antibodies.com)
  • Immunofluorescence assays demonstrated that mTOR protein is distributed throughout the cytoplasm and the nucleus at baseline in MM cell lines and in plasma cells of 13 MM patients and that pomalidomide facilitated the shift of the mTOR protein in the nucleus. (impactjournals.com)
  • The BAD protein is a pro-apoptotic member of the Bcl-2 family whose ability to heterodimerize with survival proteins such as Bcl-X(L) and to promote cell death is inhibited by phosphorylation. (elsevier.com)
  • Monoclonal antibodies were generated against the human BAD protein and used to evaluate its expression by immunoblotting and immunohistochemistry in normal human tissues and by immunoblot analysis of the National Cancer Institute anti-cancer drug screening panel of 60 human tumor cell lines. (elsevier.com)
  • Immunostaining of tissues revealed many examples of cell-type-specific expression of BAD, suggesting dynamic regulation of BAD protein levels in vivo. (elsevier.com)
  • The relative levels of BAD protein varied widely among established human tumor cell lines, with colon, lung, and melanomas generally having the highest expression. (elsevier.com)
  • As a group, hematopoietic and lymphoid lines contained the least BAD protein. (elsevier.com)
  • The BAD protein derived from 11 of 41 tumor lines that expressed this pro-apoptotic protein migrated in gels as a clear doublet, consistent with the presence of hyperphosphorylated BAD protein. (elsevier.com)
  • Taken together, these findings define for the first time the normal cell-type-specific patterns of expression and intracellular locations of the BAD protein in vivo and provide insights into the regulation of this pro-apoptotic Bcl-2 family protein in human tumors. (elsevier.com)
  • Reed, John C. / Expression and location of pro-apoptotic bcl-2 family protein BAD in normal human tissues and tumor cell lines . (elsevier.com)
  • In this study, we examine the effects of protein kinase A (PKA) and protein kinase C (PKC) on the androgen regulation of PSA in a human adenocarcinoma cell line, LNCaP. (elsevier.com)
  • In summary, the androgenic regulation of PSA protein and mRNA is repressed by tumor-promoting phorbol esters through the PKC pathway. (elsevier.com)
  • By Western blotting analysis hMSH2 protein was detected in all the tumor samples analyzed and in eight out of nine human ovarian cancer cell lines, while hMLH1 was undetectable in four out of 20 ovarian tumors and in five out of nine human ovarian cancer cell lines analyzed. (oup.com)
  • Cancer vaccines can be cell-based, protein- or peptide-based, or gene-based (DNA/RNA). (wikipedia.org)
  • in agreement with previous studies, 5 of 5 neuroblastoma and 2 of 3 melanoma cell lines also reacted with DMAb-20, GD2 was proportionally increased in the glioma and medulloblastoma cell lines relative to levels in normal brain, as determined by densitometric analysis. (nih.gov)
  • Cell lines were successfully established for nearly half of patients, with the highest success rates in melanoma, renal cell, sarcoma, and glioblastoma. (springer.com)
  • For patients with melanoma and renal cell, who were treated with TCV, observed 5-year survival rates were nearly three times longer than national figures. (springer.com)
  • Cornforth AN, Lee GJ, Fowler AW, Carbonell DJ, Dillman RO (2009) Increases in serum TARC/CCL17 levels are associated with progression-free survival in advanced melanoma patients in response to dendritic cell-based immunotherapy. (springer.com)
  • Cornforth AN, Fowler AW, Carbonell DJ, Dillman RO (2011a) Resistance to the proapoptotic effects of interferon-gamma on melanoma cells used in patient-specific dendritic cell immunotherapy is associated with improved overall survival. (springer.com)
  • Cornforth AN, Fowler AW, Carbonell DJ, Fan E, Dillman RO (2011b) Characterization of interferon-γ-treated melanoma tumor cells for use in dendritic cell-based immunotherapy. (springer.com)
  • Screening malignant melanoma cell lines against nucleoside analogs revealed as high sensitivity to fludarabine, clofarabine, and gemcitabine as to leukemic cells, and especially in those cells expressing high levels of the mitochondrial enzyme deoxuguanosine kinase (dGK). (diva-portal.org)
  • Downregulation of dGK in the melanoma cell line RaH5 using siRNA led to a compensatory increase in TK2 activity, which led to significantly increased sensitivity of the cells to gemcitabine. (diva-portal.org)
  • The compensatory regulation of deoxynucleoside kinases with over-lapping substrate specificity differed in leukemic and melanoma cell lines probably because they preferably rely on different deoxynucleoside kinases for nucleoside and nucleoside analog activation. (diva-portal.org)
  • dGK and TK2 that are both located in the mitochondria, seems to be able to compensate for each other to a higher extent in the dGK-dependent melanoma cells compared to CEM cells that possess high dCK activity. (diva-portal.org)
  • For this proof-of-concept study, keyhole limpet hemocyanin (KLH) was used as the foreign antigen and A375 melanoma or U87-MG glioblastoma cells were subcutaneously engrafted into CD34-humanised NCG mice to establish the solid tumour models. (leidenbiosciencepark.nl)
  • Canvaxin, which incorporates three melanoma cell lines, failed phase III clinical trials. (wikipedia.org)
  • Analyses showed that these newly created "mesoderm progenitors" lacked tumor-forming potential, but retained the capacity to differentiate into specific kinds of tissue, such as cells that comprise the adult kidney. (ucsd.edu)
  • Your surgeon may also remove surrounding tissue to make sure no cancer cells have spread. (mainlinehealth.org)
  • It overcomes many of the limitations of alternative approaches that are often ineffective in isolating tumor-derived exosomes from those derived from normal tissue because of the low recovery yields and the time required for the process. (eurekalert.org)
  • Phospholipid hydroperoxide GPX activity was not found to be generally elevated above normal tissue levels in newly established breast cancer-derived cell lines. (unboundmedicine.com)
  • Therefore, we determined the HLA-DR expression in 50 primary tumors, in 45 specimens of non-transformed renal tissue of tumor bearing kidneys and in two newly established cell lines (DNT-11 and DNT-63) derived from metastases by indirect immunofluorescence. (spandidos-publications.com)
  • Cytochrome P450 (P450) enzyme expression patterns were determined for a panel of 60 human tumor cell lines, representing nine tumor tissue types, used by the National Cancer Institute (NCI) Anticancer Drug Screening Program. (aspetjournals.org)
  • The expression of P450 enzymes in tumor tissue can have a major impact on the responsiveness of tumors to cancer chemotherapeutic drugs, owing to the central role that these enzymes play in the metabolism of numerous clinically useful anticancer agents ( LeBlanc and Waxman, 1989 ). (aspetjournals.org)
  • 9 ) reported that a sapphyrin derivative was cytotoxic to Jurkat cells in tissue culture and suggested that sapphyrins or other porphyrin-like systems might display useful anticancer properties even in the absence of light. (aacrjournals.org)
  • Benefits of luciferase bioluminescence in cell tracking include penetration of tissue for non-invasive monitoring and the re-usability of the enzymatic marker. (bio-protocol.org)
  • Detection of the same CDH1 241ins4 mutation in archival tumour tissue of the corresponding primary ILBC proved the clonal origin of IPH-926 from this particular tumour. (ovid.com)
  • These results show that a xenograft model using multiple cell lines of diverse tissue origin can identify common tumorigenic cell surface or secreted molecules that may be important biomarker and therapeutic discoveries. (biomedcentral.com)
  • The process of tumorigenesis has long been recognized to depend upon complex interactions of a tumor with its non-transformed tissue environment [ 1 ]. (biomedcentral.com)
  • Similarly, alterations in TH signaling have been observed in malignancies [ 3 , 4 ], and hypothyroidism is shown to enhance tumor invasiveness and metastasis development [ 5 , 6 ]. (hindawi.com)
  • Moreover, the miR-200b/200a/429 cluster prevented lung metastasis in an experimental metastasis model and although tumor initiation was not prevented, re-expression of the miR-200b/200a/429 cluster induced a dormancy-like state where mammary tumors failed to grow beyond ˜150mm3 or grew extremely slowly following intra-mammary injection. (ovid.com)
  • Therefore, re-expression of the miR-200b/200a/429 cluster in the claudin-low mammary tumor cell line, RJ423, is sufficient to alter cell morphology, impair metastasis and induce tumor dormancy. (ovid.com)
  • However, cancers are able to develop mechanisms to escape NK cell attack or to induce defective NK cells. (hep.com.cn)
  • Cell lines derived from human tumors for vaccine manufacture have been approved by FDA for trials although it is certainly not convincing (to me at least) that these vaccines will not induce tumors in the recipients, nor that the vaccines will be free from advantageous agents from the substrates. (sanevax.org)
  • The study demonstrated that CCRF-CEM cells failed to induce synergistic response with any of the investigated chemotherapies, but importantly no inhibition was observed either. (omicsonline.org)
  • 100-fold reduced cloning efficiencies in semisolid medium relative to HeLa cells and failed to induce s.c. tumors when injected into nude mice. (aacrjournals.org)
  • mTOR inhibitors are therefore considered effective anti-tumor reagents but they may also induce an activation of PI3K/Akt and MAPK pathways as a compensatory action and reduce their own anti-tumor activities. (enets.org)
  • In the future, anti-cancer drug development is likely to use a combination of molecular, cell line, primary or early passage cell culture, and xenograft methods for lead optimisation before clinical trials are contemplated. (strath.ac.uk)
  • In xenograft tumours, IPH-926 cells recapitulated the linear cord invasion pattern that defines ILBCs. (ovid.com)
  • theThe antitumor effects of physalin A were also validated usingin an in vivo mouse xenograft models of NSCLC cells. (oncotarget.com)
  • These methods suffer from inherent limitations such as the need to control immune response in the transplant animal, and the significant difference in environmental conditions from the primary tumour site to the xenograft site (e.g. absence of required exogenous molecules or cofactors). (wikipedia.org)
  • This is based on the finding of stable hgpx1 gene expression during serial culture of ER-negative breast cancer cell lines newly isolated from malignant effusion and absence of hgpx1 mRNA expression in COH-BR-5. (unboundmedicine.com)
  • only one (DMAb-20) was subsequently shown by analysis with a panel of defined ganglioside species to be specific for the minimum epitope of GD2 GalNAc beta 1-4(NeuAc alpha 2-8-NeuAc alpha 2-3)Gal-, DMAb-20 was used to evaluate the expression of GD2 by malignant glioma and medulloblastoma cell lines using cell surface radioimmunoassay. (nih.gov)
  • In a phenotypic survey of malignant glioma biopsies, tumor cells in 24 of 30 (80%) cases stained positively with DMAb-20. (nih.gov)
  • The cells from each different malignant cell line are grown on slides, followed by a paraformaldehyde fixation. (spie.org)
  • The current study explores novel targeted therapies for malignant pheochromocytomas (PCCs) and paragangliomas, utilizing a more benign (MPC) and a more malignant (MTT) PCC cell line. (enets.org)
  • Cuboidal cells were poorly tumorigenic, but elongated cells produced highly malignant sarcoma-like tumors. (stanford.edu)
  • In the cuboidal and elongated cells no expression of int-1 was detectable, showing that the continued expression of int-1 was not required for progression to more malignant cells. (stanford.edu)
  • RASSF1A methylation was tumor specific and was absent in adjacent non-malignant tissues. (nih.gov)
  • Therefore, we recently have generated five immortal human prostate epithelial cell cultures derived from both the benign and malignant tissues of prostate cancer patients with telomerase, a gene that prevents cellular senescence. (elsevier.com)
  • Furthermore, the chromosome alterations observed in these immortalized cell lines expressing aspects of the malignant phenotypes imply that these cell lines accurately recapitulate the genetic composition of primary tumors. (elsevier.com)
  • Linear expansion is less likely to reflect the endpoint of a malignant tumour because the accumulation of mutations is stochastic in heterogeneic tumours. (wikipedia.org)
  • However, T3 increased cell proliferation in 1321N1 (2 days) which declined thereafter (4 days) while in U87MG resulted in suppression of cell proliferation. (hindawi.com)
  • In conclusion, T3 can re-differentiate glioma tumor cells, whereas its effect on cell proliferation appears to be dependent on the type of tumor cell line with aggressive tumors being more sensitive to T3. (hindawi.com)
  • It is now recognized that thyroid hormone (TH) may have a critical role in the pathogenesis and the progression of the diseases due to its regulatory action on cell differentiation, proliferation, and survival [ 1 ]. (hindawi.com)
  • However, recent experimental studies provide evidence showing that acute, short-term TH treatment may increase cell proliferation and survival via its nongenomic action [ 11 - 13 ]. (hindawi.com)
  • In contrast, long-term TH treatment appears to suppress cell proliferation in neuroblastoma cells [ 5 ]. (hindawi.com)
  • Cianfanelli V, Fuoco C, Lorente M, Salazar M, Quondamatteo F, Gherardini PF et al (2015) AMBRA1 links autophagy to cell proliferation and tumorigenesis by promoting c-Myc dephosphorylation and degradation. (springer.com)
  • Cellular proliferation was determined via ATP quantification using the Cell Titer Glo assay. (omicsonline.org)
  • Metabolic viability was assessed by cell proliferation assay (MTS). (enets.org)
  • Beyond transformation and increased proliferation, many pathways are activated both in the growing tumor and its environment to culminate in an established solid tumor. (biomedcentral.com)
  • This study was designed to determine if and how a non-toxic, naturally occurring bioflavonoid, galangin, affects proliferation of human mammary tumor cells. (biomedcentral.com)
  • Because some current breast cancer therapeutics are ineffective in estrogen receptor (ER) negative tumors and since the AhR may be involved in breast cancer proliferation, the effects of galangin on the proliferation of an ER - , AhR high line, Hs578T, were studied. (biomedcentral.com)
  • The effects of these agents on cell proliferation were studied by 3 H-thymidine incorporation and by flow cytometry. (biomedcentral.com)
  • Constitutive and environmental chemical-induced AhR activity was profoundly suppressed by galangin as was cell proliferation. (biomedcentral.com)
  • However, the failure of α-NF or FhAhRR transfection to block proliferation indicated that galangin-mediated AhR inhibition was either insufficient or unrelated to its ability to significantly block cell proliferation at therapeutically relevant doses (IC 50 = 11 μM). (biomedcentral.com)
  • Galangin is a strong inhibitor of Hs578T cell proliferation that likely mediates this effect through a relatively unique mechanism, suppression of cyclin D3, and not through the AhR. (biomedcentral.com)
  • These studies investigated the effects of retinoic acids on endothelial cell proliferation. (usu.edu)
  • cis retinoic acid decreased endothelial cell proliferation. (usu.edu)
  • Most importantly, the conditioned media from tumor cells treated with low concentrations of all- trans or 9- cis retinoic acids significantly increased endothelial cell proliferation. (usu.edu)
  • Burgess LC and Hall JO: Conditioned Media from Solid Tumor Cell Lines Treated with Retinoic Acids both Decreased and Increased Proliferation of Capillary Endothelial Cells. (usu.edu)
  • Its and determined whether it effect oninhibited both constitutive and induced STAT3 activity, through repressing the phosphorylation levels of JAK2 and JAK3, resulting in anti-proliferation and pro-apoptotic effects on NSCLC cells was also determined, and. (oncotarget.com)
  • A tumor suppressor gene DIRAS1 was upregulated after SB treatment, displaying its anti-cancer potential at molecular level. (springer.com)
  • Infiltrating lobular breast cancer (ILBC) is a clinically and biologically distinct tumour entity defined by a characteristic linear cord invasion pattern and inactivation of the CDH1 tumour suppressor gene encoding for E-cadherin. (ovid.com)
  • Together with its functional properties and chromosomal localization, these findings substantiate ZAC as a good candidate for the tumor suppressor gene on 6q24-q25. (cnrs.fr)
  • We have evidence to suggest that one cell line, COH-BR-5 (ER-negative), lacked hgpx1 gene expression prior to culture. (unboundmedicine.com)
  • For example, gastrointestinal stromal tumors (GIST) that harbor activating mutations in the c-KIT gene are sensitive to treatment with imatinib mesylate, a tyrosine kinase inhibitor, whereas those without c-KIT mutations are less sensitive ( 1 ). (aacrjournals.org)
  • Disruption of the Rb cell cycle control pathway occurs in 80% of adult GBM through loss of p16 INK4a function via gene deletion, amplification of CDK4, or mutation in Rb ( 2 , 3 ). (aacrjournals.org)
  • DMS and DNase I footprinting identified protected G residues in the Pit-1, Sp1, and Zn-15 binding sites of the GH gene in GC, but not in 235-1, cells. (asm.org)
  • We conclude that the GH gene is accessible to specific nuclear proteins in GC, but not in 235-1, cells and that T3 enhances this interaction, although there is no evidence of TR binding to the low-affinity rat GH TRE. (asm.org)
  • For instance, explant and three-dimensional models allow cell:cell interactions to be examined in live cells, and endpoints can include the measurement of gene expression and image analysis. (strath.ac.uk)
  • The Effect of the Gene Encoding EcoRII DNA Methyltransferase on the Phenotype of Transgenic Tumor. (deepdyve.com)
  • 2004-10-10 00:00:00 Several tumor cell lines were obtained by transforming Nicotiana tabacumplants with the recombinant Ti plasmid comprising the gene encoding EcoRII DNA methyltransferase (M·EcoRII) and subjected to analysis. (deepdyve.com)
  • Southern blot-hybridization showed that the M·EcoRII gene was present in the cells of all transformed lines. (deepdyve.com)
  • Correlation of Ataxia-Telangiectasia-Mutated (ATM) gene loss with outcome in head and neck squamous cell carcinoma. (semanticscholar.org)
  • Expression of an exogenous tumor-necrosis-factor (TNF) gene in TNF-sensitive cell-lines confers resistance to TNF-mediated cell-lysis. (ugent.be)
  • To characterize the target cell for the oncogenic action of int-1, we have isolated permanent cell lines with distinct morphologies and differentiation characteristics, starting from a tumor with a rearranged int-1 gene. (stanford.edu)
  • In all lines, the int-1 gene was identically rearranged due to insertion of proviral DNA of the Mouse Mammary Tumor Virus, but the expression of int-1 varied with the state of differentiation of the cells. (stanford.edu)
  • Aberrant promoter methylation and silencing of the RASSF1A gene in pediatric tumors and cell lines. (nih.gov)
  • The most frequently methylated gene in both primary tumors and cell lines was RASSF1A (40, 86%, respectively). (nih.gov)
  • Relating hepatocellular carcinoma tumor samples and cell lines using gene expression data in translational research. (escholarship.org)
  • All the cell lines presented the normal alleles for the BAX gene while only in one of the tumor samples a heterozygous frameshift mutation was found. (oup.com)
  • The results indicate that frameshift mutations in the BAX gene, possibly arising as a consequence of microsatellite instability (detectable in these tumors), is detectable in human ovarian cancer although quantitatively it does not appear to be a major determinant of the low apoptotic response to chemotherapy observed in ovarian cancer cells. (oup.com)
  • T3 had no effect on ERK activation in both cell lines but significantly increased phospho-Akt levels in 1321N1. (hindawi.com)
  • AQP5 knockdown in H1299 cells significantly decreased cell migration compared with untransfected cells, as demonstrated by both Transwell and wound closure assays. (spandidos-publications.com)
  • The supernatant of AQP5‑downregulated cells exhibited significantly low tube formation potential compared with untransfected cells. (spandidos-publications.com)
  • There were no differences in cyclin D1 and E expression between the two cell lines after estrogen treatment, but the expression of p16/INK4a and p27/KIP1 was significantly higher in the OC-1170-VGH cell line than in the OVCAR3 cell line. (biomedsearch.com)
  • Patients whose treatment was escalated to chemotherapy based on their circulating tumor cell count had a significantly longer progression-free survival (median = 10.5 months with hormone therapy in the clinically driven arm in patients with a high circulating tumor cell count vs 15.5 months with chemotherapy in the circulating tumor cell arm) and showed a trend toward longer overall survival-37.1 vs 42 months, respectively. (ascopost.com)
  • An analysis that focused on the two subgroups of 292 patients with discordant treatment recommendations showed that patients treated with front-line chemotherapy had a significantly longer progression-free survival (34% less likely to experience disease progression) and overall survival (35% lower risk of death). (ascopost.com)
  • 4) The expression of several cell adhesion molecules by FACS analysis showed that the incidence of α_1 and α_2 integrins expression in AZL5G cells was significantly higher than in AZ521. (nii.ac.jp)
  • We have previously shown that the dual PI3K/mTORC1 inhibitor NVP-BEZ235 significantly reduced MPC and MTT cell viability, but increased ERK signalling. (enets.org)
  • Notably, mice from the KLH/KLH loaded DCOne-treated group produced significantly more anti-KLH IgG antibodies than the PBS control group confirming that the vaccination approach triggered a robust humoral immune response with long-term immunity and reactivity towards the antigen used for tumour marking. (leidenbiosciencepark.nl)
  • Knockdown of STAT3 expression by small interfering RNA (siRNA) significantly enhanced the pro-apoptotic effects of physalin A in NSCLC cells. (oncotarget.com)
  • In addition, the comparison of investigated blended mushroom extracts with three well-known commercial mushroom products derived from single mushroom species or single mushroom compounds shows that blended mushroom extracts exhibit significantly stronger cytotoxic effects on human tumor cell lines. (begellhouse.com)
  • Such treatment is associated with encouraging long-term survival rates, and results are superior in patients treated with DCV loaded with antigen from such cells. (springer.com)
  • Additionally, genetically modifying NK cell lines by increasing their expression of cytokines and engineering chimeric tumor antigen receptors could improve their specificity and cytotoxicity. (hep.com.cn)
  • Combining a vaccination against a highly-immunogenic foreign antigen and planting the same antigen directly into the tumour-microenvironment using our DCOne cell line as a vehicle represents a lean and elegant therapeutic strategy to break immunotolerance. (leidenbiosciencepark.nl)
  • The therapeutic strategy described in DCprime's SITC presentation is based on two key elements: inducing immunity against a foreign antigen and flagging the tumour as a target for the induced immune response with the same foreign antigen. (leidenbiosciencepark.nl)
  • A tumor antigen vaccine may stimulate the body's immune system to find and kill cancer cells. (wikipedia.org)
  • As such, tumor antigen vaccines are a type of cancer immunotherapy. (wikipedia.org)
  • Tumor antigen vaccines work the same way that viral vaccines work, by training the immune system to attack cells that contain the antigens in the vaccine. (wikipedia.org)
  • The difference is that the antigens for viral vaccines are derived from viruses or cells infected with virus, while the antigens for tumor antigen vaccines are derived from cancer cells. (wikipedia.org)
  • Antigen-presenting cells (APCs) such as dendritic cells take up antigens from the vaccine, process them into epitopes, and present the epitopes to T-cells via Major Histocompatibility Complex proteins. (wikipedia.org)
  • In this strategy, the antigen-presenting dendritic cells directly stimulate T-cells rather than relying on processing of the antigens by native APCs after the vaccine is delivered. (wikipedia.org)
  • T3 promoted re-differentiation in both cell lines. (hindawi.com)
  • In a new study published November 10, 2015 in the online journal eLIFE , researchers at University of California, San Diego School of Medicine describe a new "progenitor cell" capable of unlimited expansion and differentiation into mature kidney cells, but without the risk of forming tumors. (ucsd.edu)
  • Progenitor cells are early descendants of stem cells, with more limited differentiation capacity. (ucsd.edu)
  • He said colleagues are also pursuing similar bioengineering-based approaches to generate other similar expandable progenitor cell populations capable of differentiation into mature cell types derived from other germ layers. (ucsd.edu)
  • Bai Z, Zhang Z, Ye Y, Wang S (2010) Sodium butyrate induces differentiation of gastric cancer cells to intestinal cells via the PTEN/phosphoinositide 3-kinase pathway. (springer.com)
  • Here we show that exosomes isolated from the murine prostate cancer cell line TRAMP-C1 dramatically decrease fusion and differentiation of monocytic osteoclast precursors to mature, multinucleated osteoclasts. (diva-portal.org)
  • The presence of tumor cell-derived exosomes also clearly decreased the expression of established markers for osteoclast fusion and differentiation, including DC-STAMP, TRAP, cathepsin K, and MMP-9. (diva-portal.org)
  • For the differentiation of the cell lines a principal component analysis (PCA) is performed. (spie.org)
  • Cell culture models of normal urothelial cells are important for studying differentiation, disease mechanisms and anticancer drug development. (iospress.com)
  • Beyond primary cultures with their limitations in lifespan, interindividual heterogeneity and supply, few conditionally immortalized cell lines with limited applicability due to partial transformation or impaired differentiation capacity are available. (iospress.com)
  • HBLAK cells retain some differentiation potential and respond to cytotoxic agents similar to normal urothelial cells, but contain genetic changes contributing to immortalization in urothelial tumors. (iospress.com)
  • Cell culture models of normal urothelial cells are valuable for studying urothelial physiology and differentiation, for investigating the function of genetic and epigenetic changes found in urothelial carcinoma and as controls in the evaluation of the tumor specificity of novel cancer drugs. (iospress.com)
  • Differentiation-dependent expression of provirus-activated int-1 oncogene in clonal cell lines derived from a mouse mammary tumor. (stanford.edu)
  • Polygonal cells had retained many differentiation markers of epithelial cells and produced adenocarcinomas upon transplantation in syngenic mice. (stanford.edu)
  • We established a high-throughput platform to profile 500 cell lines derived from diverse epithelial cancers for sensitivity to 14 kinase inhibitors. (nih.gov)
  • Proteomics-based identification of proteins secreted in apical surface fluid of squamous metaplastic human tracheobronchial epithelial cells cultured by three-dimensional organotypic air-liquid interface method. (labome.org)
  • The device surface was coated with anti-epithelial cell adhesion molecule antibody by just contacting the antibody solution, and a flow system including the device was established to send a cell suspension through it. (springer.com)
  • This family of microRNAs maintain mammary epithelial identity and downregulation of miR-200 expression has been associated with epithelial-to-mesenchymal transition in mammary tumors. (ovid.com)
  • The aim of this study is to characterize the effect of tumor necrosis factor-alpha (TNFalpha) on epithelial barrier function in the colonic epithelial cell line HT-29/B6. (biologists.org)
  • IPH-926 expressed various epithelial cell markers but lacked expression of E-cadherin due to a previously unreported, homozygous CDH1 241ins4 frameshift mutation. (ovid.com)
  • Two nontransformed revertants of HeLa cells, designated HA and HF, were isolated using a selection procedure based on prolonged retention of the fluorescent dye rhodamine 123 within the mitochondria of HeLa (ATCC CCL2) cells versus normal epithelial cells. (aacrjournals.org)
  • Cuboidal and elongated cells were negative for epithelial markers. (stanford.edu)
  • P450 expression in tumor cells may lead to the localized production of intracellular drug metabolites, and may thereby either increase or decrease the cytotoxicity of test chemicals being evaluated. (aspetjournals.org)
  • To better understand the signaling pathways active in sarcoma, we examined global tyrosine phosphorylation in sarcoma cell lines and human tumor samples. (aacrjournals.org)
  • Resistance to treatment may be associated with deregulation of the two major cell cycle pathways: Rb (p16 INK4a , cyclin D 1 , CDK4) and p53 (p14 ARF , MDM2, p21 WAF1 ) tumor suppressor pathways. (aacrjournals.org)
  • Alterations of these pathways occur in the majority of human cancers, implicating these two genetic pathways in tumor development ( 1 ). (aacrjournals.org)
  • In this study, we wished to examine the effect of flavopiridol on a panel of glioma cell lines, which have alterations in both the Rb and p53 tumor suppressor pathways. (aacrjournals.org)
  • CONCLUSION: Although the ER-positive and ER-negative ovarian cancer cell lines were inhibited by estrogen, the influence of cell-cycle regulatory proteins was different between the two, suggesting that the inhibitory effect of estrogen on ovarian cancer cell lines might be mediated through different pathways. (biomedsearch.com)
  • Identification of interactions between natural bioactive compounds with anti-cancer drugs may provide new pathways to target cancerous cells. (omicsonline.org)
  • These developments led us to test if MMR-deficient cells may be compromised in their ability to activate appropriate cellular signaling pathways after ionizing radiation. (semanticscholar.org)
  • For example, adhesive pathways are activated to enable transformed cells to aggregate and form a microtumor. (biomedcentral.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • They found sensitivity to GECGC in both colon cancer cell lines they tested, in cervical cancer cells and in one line of leukemia, tumor cells. (innovations-report.com)
  • Identification of genotype-correlated sensitivity to selective kinase inhibitors by using high-throughput tumor cell line profiling. (nih.gov)
  • Cells with exquisite sensitivity to EGFR, HER2, MET, or BRAF kinase inhibitors were marked by activating mutations or amplification of the drug target. (nih.gov)
  • A ) Pie chart representation of the sensitivity of 500 cancer cell lines to inhibitors of the indicated kinases after treatment for 72 h. (nih.gov)
  • Sensitivity is calculated as the fraction of viable cells relative to untreated controls. (nih.gov)
  • The sensitivity of each cell line is indicated by the increasing intensity of the green signal. (nih.gov)
  • A ) Pie chart representation of the sensitivity of 131 human lung cancer cell lines to treatment with 200 nM EGFR kinase inhibitor erlotinib. (nih.gov)
  • Colony-forming assays using human tumor cell lines demonstrated that pretreatment with 30-250 microM 6AN for 18 h resulted in increased sensitivity to the DNA cross-linking agent cisplatin, with 6-, 11-, and 17-fold decreases in the cisplatin dose that diminishes colony formation by 90% being observed in K562 leukemia cells, A549 non-small cell lung cancer cells, and T98G glioblastoma cells, respectively. (aacrjournals.org)
  • We have used cancer cell lines as a model system for examining the relationship between underlying genotypes and sensitivity to candidate anticancer agents. (aacrjournals.org)
  • P450 enzyme profiling may thus aid in interpreting the patterns of drug sensitivity and resistance in the NCI tumor cell panel, and may facilitate the identification of anticancer agents whose activity can be altered via cytochrome P450 metabolism. (aspetjournals.org)
  • The REST signature was found to be associated with drug sensitivity in neuroblastoma cell lines. (harvard.edu)
  • The WR cells, which also express collateral sensitivity to chloroethylnitrosoureas, were approximately twice as sensitive to the cytotoxic effects of MCTIC as were WS cells. (aspetjournals.org)
  • These data indicate that the collateral sensitivity of nitrogen mustard-resistant WR cells to chloroethylating drugs is in part due to the loss of guanine-O6-alkyl transferase activity which is present in the parent line. (aspetjournals.org)
  • Specific down-regulation of XIAP with RNA interference enhances the sensitivity of canine tumor cell-lines to TRAIL and doxorubicin. (uu.nl)
  • Immuno-MALDI (iMALDI) for quantifying AKT1 and AKT2 in breast and colorectal cancer cell lines and tumors. (proteincentre.com)
  • In the present work we have screened a variety of different tumor cell lines for p185HER2 expression using both enzyme-linked immunosorbent and fluorescence-activated cell sorting assays employing murine monoclonal antibodies directed against the extracellular domain of the receptor. (nih.gov)
  • To determine cytotoxic effects of single and blended mushroom extracts, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and neutral red assays were conducted using human small cell lung cancer, lung adenocarcinoma, colon cancer, and brain astrocytoma cancer cells. (begellhouse.com)
  • Gliomas represent the most common primary brain tumor and are among the most aggressive of cancers. (hindawi.com)
  • A germ layer is a primary layer of cells that form during embryogenesis. (ucsd.edu)
  • These results indicate that primary medulloblastomas express significant levels of TP73 isoforms, and suggest that they can modulate the survival and genotoxic responsiveness of medulloblastomas cells. (labome.org)
  • Directly derived from patient tumors without any genetic manipulation, these new products provide the assurance of primary cells with long-term reproducibility and scalability. (technologynetworks.com)
  • AMSBIO also offers a range of high quality cell culture media including Renaissance Essential Tumor Medium (RETM) and WIT culture media for the primary normal cell culture. (technologynetworks.com)
  • In a previous study, we reported that salmonellae possess the ability to stimulate tumor necrosis factor alpha (TNF-α) accumulation in primary human monocytes, as well as in the human promonocytic cell line U38. (asm.org)
  • Our data showed that Salmonella species can upregulate TNF-α in primary monocytes, as well as in human promonocytic cells, through a released polypeptide(s). (asm.org)
  • The primary endpoint was met with progression-free survival proving noninferior in the circulating tumor cell-driven arm, compared with the clinically driven arm. (ascopost.com)
  • We describe characteristics of the new spontaneously immortalized cell line HBLAK derived from a primary culture of uroepithelial cells. (iospress.com)
  • Primary cultures of urothelial cells, usually established from healthy ureters removed during tumor nephrectomy, provide one valuable model [ 1 ]. (iospress.com)
  • This has led to the increasing use of primary cell cultures, primary tumour cell explants, early passage cell lines, and xenografts to improve the accuracy of results during drug development. (strath.ac.uk)
  • Treatment of three of these cell lines with the methylation-interfering agent 5-azacytidine induced ZAC re-expression, In addition, Northern blot and RNase protection assay analysis of ZAC expression in 23 unselected primary breast tumors showed a reduced expression in several samples. (cnrs.fr)
  • unfortunately, explanted primary prostate cells survive only short-term in culture, and rarely immortalize spontaneously. (elsevier.com)
  • In order to increase the spectrum of tumor types potentially susceptible to monoclonal antibody-mediated anti-p185HER2 therapies, to decrease potential immunogenicity issues with the use of murine monoclonal antibodies for human therapy, and to provide the potential for antibody-mediated cytotoxic activity, a mouse/human chimeric 4D5 (chmAb 4D5) and a "humanized" 4D5 (rhu)mAb 4D5 HER2 antibody were constructed. (nih.gov)
  • In contrast, exosomes derived from murine fibroblastic cells did not affect osteoclast formation. (diva-portal.org)
  • We characterize the first glial cell line derived from v-src transgenic mice, Tu-2449 in comparison with a virally induced murine glioma, SRB-10, and a spontaneous murine glioma, P497. (uzh.ch)
  • To test this hypothesis, the miR-200b/200a/429 cluster was re-expressed in a murine claudin-low cell line, RJ423. (ovid.com)
  • Murine tumor models have been critical to advances in our knowledge of tumor physiology and for the development of effective tumor therapies. (bio-protocol.org)
  • In estrogen receptor (ER)-positive breast cancer cell lines, very low expression of glutathione peroxidase-1 (GPX-1) activity and hgpx1 mRNA has been observed. (unboundmedicine.com)
  • VL - 55 IS - 4 N2 - In estrogen receptor (ER)-positive breast cancer cell lines, very low expression of glutathione peroxidase-1 (GPX-1) activity and hgpx1 mRNA has been observed. (unboundmedicine.com)
  • The results suggest that this non-toxic bioflavonoid may be useful as a chemotherapeutic, particularly in combination with agents that target other components of the tumor cell cycle and in situations where estrogen receptor-specific therapeutics are ineffective. (biomedcentral.com)
  • However, this action has not been investigated in estrogen receptor (ER)-negative breast tumour cells, although activation of the glucocorticoid receptor (GR) is associated with a worse prognosis in ER-negative breast cancers. (qub.ac.uk)
  • During the last decade, tumor cell-derived microvesicles have been identified and suggested to be involved in cancer disease progression. (diva-portal.org)
  • This further strengthens the role of tumor cell-derived microvesicles in cancer progression and disease aggressiveness. (diva-portal.org)
  • We have measured mRNA expressions and enzymatic activities and correlated them with the cytotoxic response to nuc1eoside analogs and changes in cell cycle progression. (diva-portal.org)
  • In contrast, patients whose treatment was de-escalated to hormone therapy based on their circulating tumor cell count had nonsignificantly shorter progression-free survival and overall survival compared with patients who received chemotherapy in the clinically driven arm with a low circulating tumor cell count. (ascopost.com)
  • The signal transducers and activators of transcription 3 (STAT3) signaling pathway plays critical roles in the pathogenesis and progression of various human cancers, including non-small cell lung cancer (NSCLC). (oncotarget.com)
  • The long-term mutational accumulation may provide a selective advantage during certain stages of tumour progression. (wikipedia.org)
  • Despite reduced levels of several NER proteins in the testis cancer cell lines, a proficient removal of UVC-induced DNA lesions by NER was observed using an alkaline comet assay. (uio.no)
  • The tumor model simulates different malignancies by controlled expression of the tumor suppressor proteins PTEN and TP53 within the cell lines derived from the wild type. (spie.org)
  • In this study, we investigated the changes in cell-cycle regulatory proteins in ovarian cancer cell lines after estrogen treatment to explore the role of estrogen in ovarian cancers. (biomedsearch.com)
  • The cell-cycle regulatory proteins, including cyclin D1, cyclin E, p16/INK4a, and p27/KIP1, were used to check the possible mechanism of an estrogen effect on survival of the cancer cell line. (biomedsearch.com)
  • 1989 ). Tumor necrosis factor/cachectin increases permeability of endothelial cell monolayers by a mechanism involving regulatory G proteins. (biologists.org)
  • Western blot analyses of immunoprecipitated human papillomavirus 18 E6 and E7 proteins further demonstrated that the levels of these viral oncoproteins were comparable in HeLa cells and revertants. (aacrjournals.org)
  • In many types of normal cells, BAD immunoreactivity was associated with cytosolic organelles resembling mitochondria, suggesting that BAD is often heterodimerized with other Bcl-2 family proteins in vivo. (elsevier.com)
  • The expression of mismatch repair proteins hMSH2 and hMLH1 was investigated in human ovarian cancer cell lines and in biopsies of ovarian carcinomas obtained from 20 patients undergoing surgical operation. (oup.com)
  • Cancer-specific tumor antigens include peptides from proteins that are not typically found in normal cells but are activated in cancer cells or peptides containing cancer-specific mutations. (wikipedia.org)
  • Anti-proliferative effect of SB in renal cell carcinoma (RCC) cell line UOK146 was evaluated by MTT assay and morphological characteristics were observed by phase contrast microscopy which displayed the cell death after SB treatment. (springer.com)
  • The sphere assay showed that the sphere-forming cells were present in these tumors. (nii.ac.jp)
  • The cell surviving fraction was assessed by clonogenic assay. (physiciansweekly.com)
  • This process is believed to be mediated by the upregulation of tumor necrosis factor alpha (TNF-α) production by macrophages. (asm.org)
  • 1993 ). Modulation of human endothelial cell permeability by combinations of the cytokines interleukin-1 alpha/beta, tumor necrosis factor-alpha and interferon-gamma. (biologists.org)
  • Examination of these cell lines for their morphologies and proliferative capacities, their abilities to grow in low serum, to respond to androgen stimulation, to grow above the agar layer, to form tumors in SCID mice, suggests that they may serve as valid, useful tools for the elucidation of early events in prostate tumorigenesis. (elsevier.com)
  • It's easier to create mature cell populations for research or therapeutic use. (ucsd.edu)
  • We have established proliferating pure tumor cell cultures from cancer samples, followed by further expansion for patient-specific therapeutic purposes. (springer.com)
  • In this review, NK cells in tumor immunotherapy are discussed, and a list of therapeutic NK cell lines currently undergoing preclinical and clinical trials of several kinds of tumors are reviewed. (hep.com.cn)
  • This method provides a very convenient way to examine the effect of therapeutic drugs on the neuroendocrine tumor cells. (jove.com)
  • Thus, as an inhibitor of JAK2/3-STAT3 signaling, physalin A, has potent anti-tumor activities, which may facilitate the development of a therapeutic strategy for treating NSCLC. (oncotarget.com)
  • Therapeutic vaccines focus on killing existing tumors. (wikipedia.org)
  • 1321N1 cell line, an astrocytoma grade II, and U87MG, a glioblastoma grade IV, were used in this study. (hindawi.com)
  • Among the morphological variants of glioblastoma examined, the most intense staining with DMAb-20 was observed in neoplastic gemistocytes, with the weakest or absent staining in small cell glioblastomas. (nih.gov)
  • Glioblastoma multiforme represents a highly lethal brain tumor. (spie.org)
  • Three human neoplastic cell lines, U-373 MG glioblastoma, DU-145 prostate carcinoma, and TCCSUP bladder transitional cell carcinoma, were treated with all- trans , 9- cis , or 13- cis retinoic acids at 0.0001 to 10 μM. (usu.edu)
  • Evidence of the cancer stem cell model has been demonstrated in multiple tumour types including leukemias, glioblastoma, breast cancer, and prostate cancer. (wikipedia.org)
  • hMLH1 and hMSH2 expression and BAX frameshift mutations in ovarian cancer cell lines and tumors. (oup.com)
  • One such hypothesis is based on the high susceptibility of stem cells to accumulate mutations, together with the existence of epigenetic changes that are essential for the initiation and development of tumourgenic behaviour. (oncotarget.com)
  • A minimal level of intra-tumour heterogeneity is a simple consequence of the imperfection of DNA replication: whenever a cell (normal or cancerous) divides, a few mutations are acquired-leading to a diverse population of cancer cells. (wikipedia.org)
  • Stem cell variability is often caused by epigenetic changes, but can also result from clonal evolution of the CSC population where advantageous genetic mutations can accumulate in CSCs and their progeny (see below). (wikipedia.org)
  • In this model, tumours arise from a single mutated cell, accumulating additional mutations as it progresses. (wikipedia.org)
  • Based on previous limited surveys of breast cancer cell lines, it has been suggested that there may be an inverse correlation between ER status and GPX-1 production. (unboundmedicine.com)
  • Here we report the results from a larger survey of breast cancer cell lines, including six recently isolated cell lines. (unboundmedicine.com)
  • Expression of hgpx2 mRNA (producing GPXGI, the GI tract GPX) was detected in several long and newly established, ER-negative breast cancer cell lines. (unboundmedicine.com)
  • In these studies, the scientists tested the effects of three different doses of GECGC on the cancer cell lines - the first time that a synthetic cocoa derivative has been used to screen human cancer cell lines. (innovations-report.com)
  • We offer an extensive selection of total RNA from human tumors and human cancer cell lines. (clontech.com)
  • These studies have revealed the utility of large panels of cancer cell lines to capture the genomic heterogeneity of human cancer and to identify clinically relevant genotype-phenotype relationships. (aacrjournals.org)
  • These findings support the involvement of ATM signaling in response to cisplatin in the testis cancer cell lines. (uio.no)
  • We present a simple agarose overlay platform to grow 3D multicellular spheroids using neuroendocrine cancer cell lines. (jove.com)
  • Effects of interferons and tumour necrosis factor-a on human lung cancer cell lines and the development of an interferon-resistant lung cancer cell line. (diva-portal.org)
  • Do Cancer Cell Lines Really Resemble Tumors? (thinkpinkrocks.com)
  • Cancer cell lines are used extensively to study cancer biology and to test hypotheses in translational research. (escholarship.org)
  • Acute and chronic exposition of colon cancer cell lines to CT/CTE PEDF-derived peptides decreased drug-resistance to conventional colorectal cancer treatments, such as oxaliplatin or irinotecan. (oncotarget.com)
  • Using a combination of established cancer cell lines that resemble the patient's tumor can overcome these barriers, but this approach has yet to be effective. (wikipedia.org)
  • Both engineered antibodies, in combination with human peripheral blood mononuclear cells, elicited antibody-dependent cytotoxic responses in accordance with the level of p185HER2 expression. (nih.gov)
  • Monoclonal antibodies (mAbs) recognizing the disialoganglioside II3(NeuAc)2GgOse3Cer (GD2) were produced by immunizing mice with the GD2-expressing neuroblastoma cell line LAN-1 and a prefusion boost with purified GD2 coupled to Salmonella minnesota. (nih.gov)
  • 1990 ). Antibodies to a soluble form of a tumor necrosis factor (TNF) receptor have TNF-like activity. (biologists.org)
  • Based on this evidence, in the present study, we further explored the long-term T3 effects on glioma tumors in relation to the degree of tumor aggressiveness and potential alterations in thyroid hormone nuclear receptor (TR) expression which may characterize different types of glioma cell lines. (hindawi.com)
  • TY - JOUR T1 - Expression of selenium-dependent glutathione peroxidase in human breast tumor cell lines. (unboundmedicine.com)
  • Dadakhujaev S, Noh HS, Jung EJ, Hah YS, Kim CJ, Kim DR (2008) The reduced catalase expression in TrkA-induced cells leads to autophagic cell death via ROS accumulation. (springer.com)
  • Despite encouraging preclinical studies and studies showing receptor expression in sarcoma tumor specimens, activity of tyrosine kinase inhibitors (TKI) in patients with advanced sarcoma has been limited. (aacrjournals.org)
  • The expression of MHC class II antigens in renal cell carcinoma may be important for the response to interleukin-2 based immunotherapy. (spandidos-publications.com)
  • There was no correlation between HLA-DR expression and tumor stage or grading, respectively. (spandidos-publications.com)
  • Metallothionein expression and resistance to cisplatin in a human germ cell tumor cell line. (aspetjournals.org)
  • Expression of intracellular metallothionein (MT) has been linked to cis-diamminedichloroplatinum (cDDP) resistance in human germ cell tumor cell lines. (aspetjournals.org)
  • To determine whether exposure to cDDP would select for cells with increased MT expression, the MT content of the human teratocarcinoma cell line T7800 was measured after development of resistance to cDDP by exposure to progressively higher drug concentrations (6.25-25 microM). (aspetjournals.org)
  • These results suggest that increased MT expression is concomitant with increased cDDP resistance in a variety of human tumor cell lines. (aspetjournals.org)
  • In GC cells stably transfected with a plasmid containing F2-TRE-TK-CAT or TRalpha, chloramphenicol acetyltransferase expression was T3 inducible and DMS footprinting revealed both F2 TRE TR-binding half sites in a pattern suggesting the binding of TR homodimers before and during T3 exposure. (asm.org)
  • BCL-2 and BAX expression were lower in Tu-2449 and P497 than in SRB-10 cells. (uzh.ch)
  • 1996 ). Induction of RANTES expression by astrocytes and astrocytoma cell lines. (biologists.org)
  • Transient expression by plasmid transfection or non-integrating virus transduction limits the time over which cell tracking can be performed. (bio-protocol.org)
  • Expression of an exogenous tumor-necr. (ugent.be)
  • AhR expression and function in the presence or absence of galangin, a second AhR inhibitor, α-naphthoflavone (α-NF), an AhR agonist, indole-3-carbinol, and a transfected AhR repressor-encoding plasmid ( FhAhRR ) were studied in Hs578T cells by western blotting for nuclear (for instance, constitutively activated) AhR and by transfection of an AhR-driven reporter construct, pGudLuc . (biomedcentral.com)
  • In the sphere-forming-cuboidal cells, expression of int-1 was low but inducible by dexamethasone. (stanford.edu)
  • Willert noted that the progenitor cells developed are likely capable of differentiating into other cell types of the intermediate mesodermal lineage as well, most notably the germ line to generate eggs and sperm in a dish. (ucsd.edu)
  • Germ cells are the cells in your body that are used for reproduction. (mainlinehealth.org)
  • In women, germ cells form the eggs in the ovaries. (mainlinehealth.org)
  • In men, germ cells form the sperm in the testicles. (mainlinehealth.org)
  • Sometimes when germ cells don't fully develop, they become tumors. (mainlinehealth.org)
  • Germ cell tumors may or may not be cancerous. (mainlinehealth.org)
  • Overall, germ cell tumors are rare. (mainlinehealth.org)
  • Most germ cell tumors occur in the ovaries and testicles, forming ovarian cancer or testicular cancer. (mainlinehealth.org)
  • However, germ cell tumors can also very rarely form in other parts of your body, such as the brain or spine. (mainlinehealth.org)
  • What are the symptoms of germ cell tumors? (mainlinehealth.org)
  • Germ cell tumors may cause symptoms in children and young adults. (mainlinehealth.org)
  • How are germ cell tumors treated? (mainlinehealth.org)
  • Germ cell tumors tend to respond well to treatments. (mainlinehealth.org)
  • If germ cell tumors are cancerous, surgery is often followed by chemotherapy , which uses medicines to kill cancer cells. (mainlinehealth.org)
  • Cisplatin-based chemotherapy is known to be exceptionally effective in the treatment of testicular germ cell tumors. (uio.no)
  • In this study we investigated the DNA damage response in two testicular germ cell tumor cell lines. (uio.no)
  • Thus, this model system may be useful for analyzing synergistic interactions between tumor promoters and activated oncogenes during multistage carcinogenesis. (asm.org)
  • The NCI60 human tumour cell line anticancer drug screen. (nih.gov)
  • Such cell lines have been used as models in studies of resistance to redox cycling anticancer drugs. (unboundmedicine.com)
  • Natural killer (NK) cells are considered to be critical players in anticancer immunity. (hep.com.cn)
  • Significant negative correlations between the patterns of P450-dependent 7-benzyloxyresorufin metabolism activity and cell line chemosensitivity were observed for 10 standard anticancer agents (including 6 alkylating agents) and 55 investigational compounds, suggesting a role for P450 metabolism in the inactivation of these agents. (aspetjournals.org)
  • Although, as noted above, cytochrome P450 enzymes contribute to the metabolism of a large number of drug substrates and can have a large impact on a drug's anticancer activity, little is presently known about the P450 activity levels present in the individual tumor cell lines that constitute the NCI panel. (aspetjournals.org)
  • In the present study, the activity of sapphyrins as anticancer agents in hematopoietic-derived tumor cells was explored. (aacrjournals.org)
  • The int-1 mammary oncogene is frequently activated by proviral insertion in mouse mammary tumors. (stanford.edu)
  • Hi cell biologists, I am looking for tumour cell lines derived from childhood renal tumours such as Wilms' or the bone marrow metastasizing renal tumour of chilhood (BMRTC). (bio.net)
  • In the present study, anti-cancer activity of SB in Xp11.2 (TFE3) translocated renal cell carcinoma cell line UOK146 was studied. (springer.com)
  • Cell-based vaccines include tumor cells or tumor cell lysates. (wikipedia.org)
  • Human gastric carcinoma cell line AZ521 (5 X 10^6/0.1ml) was transplanted orthotopically into the stomach of nude mice using cell suspension technique. (nii.ac.jp)
  • To characterize utility and limitations of HBLAK cells as an urothelial cell culture model. (iospress.com)
  • Characterization of a human prostate adenocarcinoma cell line (DU 145) as a monolayer culture and as a solid tumor in athymic mice. (semanticscholar.org)
  • Both of these sapphyrins were found to have potent cytotoxic activity in hematopoietic-derived tumor cells, with PCI-2010 displaying a lower level of toxicity in mice. (aacrjournals.org)
  • Methanolic and aqueous extracts from 37 seaweed species (10 green and 27 brown seaweeds) collected from Jeju Island coast were prepared at high (70℃) and room (20℃) temperatures and examined for cytotoxic activity against 4 tumor cell lines: U937 (human monoblastoid leukemia cell line), HL60 (human promyelocytic leukemia cell line), HeLa (woman cervical carcinoma cell line) and CT26 (mouse colon carcinoma line). (dbpia.co.kr)
  • The present study investigated the potential effects of long-term T3 treatment on glioma tumor cell lines. (hindawi.com)
  • Glioma cell line U87MG was obtained from the American Type Culture Collection (ATCC) (Manassas, VA), and glioma cell line 1321N1 was obtained from the European Collection of Cell Culture (ECACC) (Salisbury, Wiltshire, UK). (hindawi.com)
  • Selective repression of YKL-40 by NF-kappaB in glioma cell lines involves recruitment of histone deacetylase-1 and -2. (labome.org)
  • Mitochondrial damage measured by cytochrome c release and transmission electron microscopy was not observed in drug-treated glioma cells. (aacrjournals.org)
  • In glioma cell lines, overexpression of MDM2 has been associated with drug resistance ( 8 , 9 ). (aacrjournals.org)
  • In clinical trials, patients were treated with a series of s.c. injections of irradiated autologous tumor cells (TCV), or autologous dendritic cells (DCV), loaded with antigens from their tumor cell line. (springer.com)
  • 1982). Further, retroviral vectors require cell division for genomic integration and can be inefficient at transducing highly differentiated cells such as neurons, dendritic cells, or resting lymphocytes. (bio-protocol.org)
  • Another cell-based vaccine strategy involves autologous dendritic cells (dendritic cells derived from the patient) to which tumor antigens are added. (wikipedia.org)
  • Unlike traditional protocols for cell line creation, these cancer models eliminate the possibility of large scale cellular adaptation through culture and genetic drift. (technologynetworks.com)
  • The DNA fingerprint of IPH-926 verified genetic identity with the patient and had no match among the human cell line collections of several international biological resource banks. (ovid.com)
  • Multiple types of heterogeneity have been observed between tumour cells, stemming from both genetic and non-genetic variability. (wikipedia.org)
  • Genetic heterogeneity is a common feature of tumour genomes, and can arise from multiple sources. (wikipedia.org)
  • These results suggest that AtT-20/D16v cells produce gamma MSH-LIs with molecular weights of 31K, 21-23K, 16-17K, 13-14K, and 3.8K, and they are secreted concomitantly with ACTH-LI and beta-EP-LI. (eurekamag.com)
  • Recently a novel mechanism, by which some genetically deregulated and aggressive tumour cells generate "micro-vascular" channels without the participation of endothelial cells and independent of angiogenesis, has been proposed. (biomedsearch.com)
  • Then CM were transferred to bovine capillary endothelial cells for 48 hours of normoxic culturing, counted and compared to controls. (usu.edu)
  • We are now investigating to what extent these cells can generate other tissues and organs that derive from intermediate mesoderm, including reproductive organs. (ucsd.edu)
  • Highly active insulin-degrading activity was found using cell suspensions of 22 cloned and 8 subcloned cell lines derived from RINm as well as 11 other continuous cell lines derived from a variety of nonislet tissues of rat, mouse, and human origin. (diabetesjournals.org)
  • Although insulin protease is present in a variety of tissues, it may have an additional regulatory function in cells that are actively synthesizing, storing, and secreting insulin. (diabetesjournals.org)
  • These results reveal that these extracts exhibit different cytotoxic effects on tumor cells originating from different tissues. (begellhouse.com)
  • Non‑small cell lung cancer (NSCLC) constitutes the majority of all lung‑cancer cases. (spandidos-publications.com)
  • The origins of these human cell lines were lung (A549), breast (MDA MB-231), colon (HCT-116), ovarian (SK-OV-3) and prostate (PC3) carcinomas. (biomedcentral.com)
  • In non-small cell lung cancers with a programmed death-ligand 1 (PD-L1) tumor proportion score (TPS) of ≥50%, first-line treatment with the PD-1 inhibitor pembrolizumab improves survival compared to platinum-doublet chemotherapy. (asco.org)
  • Here, we report a detailed homozygous deletion (HD) profiling for 246 critical loci on a panel of 89 tumor cell lines containing significant subsets of lung, ovarian and head and neck squamous cell carcinomas. (pasteur.fr)
  • Among the remaining alterations, HDs affecting TP73 or telomeric markers have never been previously described, whereas other HDs represent the first examples associating lesions of certain TSGs with a given tumor type (NF2 in lung and ovarian cells, STK11 in HELA cells). (pasteur.fr)
  • Overall, tumor cell lines established from ovarian or lung carcinomas displayed a surprising diversity of loci targeted by HDs with 7 and 6 loci involved, respectively. (pasteur.fr)
  • Dillman RO, Nayak SK, Brown JV, Mahdavi K, Beutel LD (1999) The feasibility of using short-term cultures of ovarian cancer cells for use as autologous tumor cell vaccines as adjuvant treatment of advanced ovarian cancer. (springer.com)
  • Serial concentrations of estrogen were used to evaluate the effects of estrogen on the survival of ovarian cancer cells. (biomedsearch.com)
  • ATCC announces the addition of ATCC Tumor Cell Panels to its product offering for aid in cancer research, drug discovery, biomarkers and pathway analysis. (drugdiscoverynews.com)
  • These data suggest that IL-15 has an important role in activating an NK cell-mediated pathway that leads to the elimination of intracellular protozoans from the intestines, which is a previously unrecognized feature of NK cell function. (labome.org)
  • Five of six cell lines have deletions of the CDK inhibitor p16 INK4a (all except CCF-STTG1) inactivating the Rb cell cycle control pathway. (aacrjournals.org)
  • Results of previous studies demonstrated that phosphoinositide 3-kinase (PI3K)/Akt/mTOR signaling pathway may be activated in the great majority of NET cells. (enets.org)
  • In MM cells the anti-myeloma activity of pomalidomide may be mediated by the regulation of the mTOR pathway. (impactjournals.com)
  • These cells may function in tumor initiation, aggression and recurrence. (nii.ac.jp)
  • These are the cancer stem cell model and the clonal evolution model. (wikipedia.org)

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