The science and application of a double-beam transmission interference microscope in which the illuminating light beam is split into two paths. One beam passes through the specimen while the other beam reflects off a reference mirror before joining and interfering with the other. The observed optical path difference between the two beams can be measured and used to discriminate minute differences in thickness and refraction of non-stained transparent specimens, such as living cells in culture.
A form of interference microscopy in which variations of the refracting index in the object are converted into variations of intensity in the image. This is achieved by the action of a phase plate.
Measurement of the index of refraction (the ratio of the velocity of light or other radiation in the first of two media to its velocity in the second as it passes from one into the other).
A triglyceride that is used as an antifungal agent.
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
Long, pliable, cohesive natural or manufactured filaments of various lengths. They form the structure of some minerals. The medical significance lies in their potential ability to cause various types of PNEUMOCONIOSIS (e.g., ASBESTOSIS) after occupational or environmental exposure. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p708)
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
Microscopy in which television cameras are used to brighten magnified images that are otherwise too dark to be seen with the naked eye. It is used frequently in TELEPATHOLOGY.
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
Microscopy using polarized light in which phenomena due to the preferential orientation of optical properties with respect to the vibration plane of the polarized light are made visible and correlated parameters are made measurable.
The study of the structure of various TISSUES of organisms on a microscopic level.
The recording of images in three-dimensional form on a photographic film by exposing it to a laser beam reflected from the object under study.
Computer-assisted processing of electric, ultrasonic, or electronic signals to interpret function and activity.
Anhydride polymers with a repeating structure of RC(=O)OC(=O)R. They readily hydrolyze in water making them useful for DELAYED-ACTION PREPARATIONS.
Methods of creating machines and devices.
A plant family of the order Myrtales, subclass Rosidae, class Magnoliopsida composed of tropical plants with parallel-nerved leaves.
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Cytometry Digital holographic microscopy Holographic interference microscopy Live cell imaging Phase-contrast microscopy ... Conventional phase contrast microscopy and related methods, such as differential interference contrast microscopy, visualize ... holographic interference microscopy and digital in-line holographic microscopy. Common to these methods is that an interference ... Quantitative phase contrast microscopy is primarily used to observed unstained living cells. Measuring the phase delay images ...
... are traditionally viewed in a phase-contrast microscope or in a differential interference contrast microscope. These methods ... Digital holographic microscopy makes it possible to perform cell counting and to measure cell viability directly in the cell ... digital holography quantitative phase contrast microscopy Holographic interferometry holotomography holography microscopy ... Label-free cell cycle analysis. The phase shift induced by cells has been shown to be correlated to the cell dry mass. The cell ...
Quantitative phase contrast microscopy has an advantage over fluorescent and phase contrast microscopy in that it is both non- ... Janicke B. "Digital holographic microscopy video showing cell division of unlabeled JIMT-1 breast cancer cells". The Cell - an ... Other later phase contrast techniques used to observe unstained cells are Hoffman modulation and differential interference ... live cell imaging rapidly became popular using phase contrast microscopy. The phase contrast microscope was popularized through ...
Other related microscopy method to holographic interference microscopy are phase contrast microscopy, holographic ... "Blood cell imaging by holographic method", Imaging and microscopy, 2:46-49. Tishko, T.V., Titar, V.P., Barchotkina, T.M., ... Holographic phase-contrast method is the holographic interference microscopy technique for phase micro-object visualization ... Holographic interference-contrast method is the holographic interference microscopy technique for phase micro-object ...
... phase shifting interferometry, and differential interference contrast microscopy (Nomarski microscopy); focus detection methods ... Non-scanning technologies as digital holographic microscopy enable 3D topography measurement in real-time. 3D topography is ... "Fast in-line surface topography metrology enabling stress calculation for solar cell manufacturing for throughput in excess of ... Optical methods include interferometry based methods such as digital holographic microscopy, vertical scanning interferometry/ ...
... translational moiré fringes can be seen as parallel contrast lines formed in phase-contrast TEM imaging by the interference of ... "Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy". Journal of Microscopy. ... If we consider a cell of the lattice formed, we can see that it is a rhombus with the four sides equal to d = p/sin α; (we have ... A similar effect can be obtained by the superposition of a holographic image of the object to the object itself: the hologram ...
Phase contrast and differential interference contrast (DIC) microscopy are important tools in biology and medicine. Most animal ... 24 and 25, phase contrast and DIC microscopes allow unstained, living cells to be studied.[98] DIC also has non-biological ... phase shifting techniques can be adapted to almost any system that uses fringes for measurement, such as holographic and ... For an elementary discussion, see Phase-contrast x-ray imaging (introduction). For a more in-depth review, see Phase-contrast X ...
Phase contrast illumination, sample contrast comes from interference of different path lengths of light through the sample. ... If there is no cell attached to the glass, there will be no interference. Interference reflection microscopy can be obtained by ... In digital holographic microscopy (DHM), interfering wave fronts from a coherent (monochromatic) light-source are recorded on a ... An additional technique using interference is interference reflection microscopy (also known as reflected interference contrast ...
... translational Moiré fringes can be seen as parallel contrast lines formed in phase-contrast TEM imaging by the interference of ... Microscopy[edit]. In super-resolution microscopy, the moiré pattern can be used to obtain images with a resolution higher than ... See also: theory of elasticity, strain tensor and holographic interferometry. Image processing[edit]. Some image scanner driver ... If we consider a cell of the lattice formed, we can see that it is a rhombus with the four sides equal to d = p/sin α; (we have ...
For illumination techniques like dark field, phase contrast and differential interference contrast microscopy additional ... Courjon, D.; Bulabois, J. (1979). "Real Time Holographic Microscopy Using a Peculiar Holographic Illuminating System and a ... concepts in optical microscopy Online tutorial of practical optical microscopy OpenWetWare Cell Centered Database. ... Phase contrast illumination, sample contrast comes from interference of different path lengths of light through the sample. ...
... and Medicine in Frontiers in Physics quantitative phase imaging digital holographic microscopy holography Wolf, Emil (1969). " ... "Combining Three-Dimensional Quantitative Phase Imaging and Fluorescence Microscopy for the Study of Cell Pathophysiology". Yale ... Because the RI can serve as an intrinsic imaging contrast for transparent or phase objects, measurements of RI tomograms can ... Baber, R. (1952). "Interference microscopy and mass determination". Nature. 169 (4296): 366-7. Bibcode:1952Natur.169..366B. doi ...
High resolution phase-contrast x-ray image of a spider Phase contrast and differential interference contrast (DIC) microscopy ... 24 and 25, phase contrast and DIC microscopes allow unstained, living cells to be studied. DIC also has non-biological ... such as holographic and speckle interferometry. In coherence scanning interferometry, interference is only achieved when the ... For an elementary discussion, see Phase-contrast x-ray imaging (introduction). For a more in-depth review, see Phase-contrast X ...
Named after Frits Zernike, the Dutch optical physicist, and the inventor of phase contrast microscopy, they play an important ... A uniflagellar sperm cell that is motile is referred to as a "spermatozoon", whereas a non-motile sperm cell is referred to as ... The holographic principle is a property of string theories and a supposed property of quantum gravity that states that the ... The interference experiments of Thomas Young vindicated Huygens' s wave theory in 1801. In 1678, Huygens discovered the ...
... yielding constructive interference. Both SB and RB will have undergone a phase shift of (1 × wavelength + k) due to two front- ... 1, a compensating cell made of the same type of glass as the test cell (so as to have equal optical dispersion) would be placed ... In contrast to the well-known Michelson interferometer, each of the well-separated light paths is traversed only once. If the ... Francois Bruno; Jérôme Laurent; Daniel Royer; Michael Atlan (2014). "Holographic imaging of surface acoustic waves". Applied ...
"Quantitative phase contrast microscopy - label-free live cell imaging and quantification". Phase Holographic Imaging AB.. ... Niu XY, Peng ZL, Duan WQ, Wang H, Wang P (2006). "Inhibition of HPV 16 E6 oncogene expression by RNA interference in vitro and ... The sub images were extracted from a 61-hour time-lapse microscopy video, created using quantitative phase-contrast microscopy ... HeLa cells are an immortalized cancer cell line used frequently in research. The cell line was established by removing cells ...
... such as atomic force microscopy (Raman-AFM) and scanning electron microscopy (Raman-SEM) to compare Raman distribution maps ... Taking the cell culture example, a hyperspectral image could show the distribution of cholesterol, as well as proteins, nucleic ... Raman scattering also contrasts with infrared (IR) absorption, where the energy of the absorbed photon matches the difference ... This may still be used to record very small Raman shifts as holographic filters typically reflect some of the low frequency ...
Multipass spectroscopic absorption cells. *Multiphoton lithography. *Multiple-prism dispersion theory. *Multiple-prism grating ... Group delay and phase delay. *Group velocity. *Group velocity dispersion. *Guided-mode resonance ... Minimum resolvable contrast. *Minnaert function. *Lazzaro Mongiardini. *Monochromatic aberration. *Morphology-dependent ...
K. F. A. Ross, Phase Contrast and Interference Microscopy for Cell Biologists (Edward Arnold Publishers, London, 1967). ... K. F. A. Ross, Phase Contrast and Interference Microscopy for Cell Biologists (Edward Arnold Publishers, London, 1967). ... K. F. A. Ross, Phase Contrast and Interference Microscopy for Cell Biologists (Edward Arnold Publishers, London, 1967). ... "Quantitative phase-contrast imaging of cells with phase-sensitive optical coherence microscopy," Opt. Lett. 29(13), 1509-1511 ( ...
Cytometry Digital holographic microscopy Holographic interference microscopy Live cell imaging Phase-contrast microscopy ... Conventional phase contrast microscopy and related methods, such as differential interference contrast microscopy, visualize ... holographic interference microscopy and digital in-line holographic microscopy. Common to these methods is that an interference ... Quantitative phase contrast microscopy is primarily used to observed unstained living cells. Measuring the phase delay images ...
The classical unlabeled phase contrast imaging methods such as differential interference contrast (DIC) and digital holographic ... 10887, Quantitative Phase Imaging V. KEYWORDS: Confocal microscopy, Eye, Holography, Phase contrast, Digital holography, Retina ... Quantitative phase imaging of retinal cells (Conference Presentation) Timothé LaForest, Dino Carpentras, Laura Kowalczuk, ... In digital holographic microscopy (DHM), the long coherence length of laser light causes parasitic interferences due to ...
Quantitative phase contrast microscopes can provide both intensity and phase profiles of the cells under study. The phase ... The lateral shearing phase shifting interference microscopy with low coherent illumination for tomography of living cells has ... Identification of malaria infected red blood samples by digital holographic quantitative phase microscope Author(s): Nimit R. ... We review our technique for tomographic phase microscopy with optical tweezers [1]. This tomographic phase microscopy approach ...
... enabling acquisition of information such as phase, which cannot otherwise be captured. ... Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells ... with contrast methods such as Zernike phase contrast and differential interference contrast (DIC). Such complete acquisition of ... Recently, a desire to understand live cell dynamics has rekindled interest in label-free phase contrast microscopy. This ...
... phase image. The produced quantitative phase image may have sufficient contrast for label-free auto-segmentation of cell bodies ... images with various relative phase shifts between light diffracted by a sample and light not diffracted by the sample to ... Systems and methods described herein employ multiple phase-contrast ... Conventional phase-contrast imaging techniques (e.g., Zernike, different interference contrast (DIC)) enable imaging of cell ...
Holographic, phase contrast or differential interference contrast (DIC) microscopes have been implemented especially for making ... Quantum dots (Q-dots) label structures that can be observed in live cell mode by fluorescence microscopy. As light microscopy ... We, the Imaging & Microscopy team, would like to present you again the kindly provided winning pictures of this ... ... Lens-Free Microscopy: A Light Microscope Made Only with Consumer Electronic Products. ...
3 Quantitative phase image of a red blood cell smear obtained by means of a digital holographic microscope. ... 2 Quantitative phase image of a drosophila melanogaster head cut obtained by digital holographic microscopy. ... There are mainly two groups of phase-imaging techniques: the first group, known as phase-contrast techniques, involves the ... which only requires recording the interference between a beam diffracted by the sample and a free-space propagated beam. If the ...
Autofocusing in digital holographic phase contrast microscopy on pure phase objects for live cell imaging. Appl Opt. 47, (19), ... Majeed, H., et al. Breast cancer diagnosis using spatial light interference microscopy. J Biomed Opt. 20, (11), 111210 (2015). ... Modular digital holographic microscopy system for marker free quantitative phase contrast imaging of living cells. Proc. SPIE. ... Integral refractive index determination of living suspension cells by multifocus digital holographic phase contrast microscopy. ...
... differential interference contrast (DIC) microscopy, Hoffmans microscopy, and Zernikes phase-contrast microscopy (2, 3). ... Holographic videos of sperm cells were acquired by off-axis holographic phase microscopy, which allows high-resolution complex ... Identification of bovine sperm head for morphometry analysis in quantitative phase-contrast holographic microscopy. Opt. ... Interferometric phase microscopy for label-free morphological evaluation of sperm cells. Fertil. Steril. 104, 43-47.e2 (2015). ...
... imaging of phase microscopic objects. Classical and holographic methods of phase, interference and polarization contrast are ... The first 3D images of native phase microscopic objects such as blood cells were obtained using the DHIM. The results of DHIM ... Holographic Microscopy of Phase Microscopic Objects: Theory and Practice (Hardcover) By Titar Vladimir, Tatyana Tishko, Tishko ... The book presents a clear and comprehensive review of the current status of the holographic microscopy with discussion of the ...
... called spatial light interference microscopy (SLIM) - offers ne ... A new imaging method that can measure cell mass using two beams ... Unlike most other cell-imaging techniques, SLIM - a combination of phase-contrast microscopy and holography - does not need ... In the holographic process, a coherent beam first is split into two component beams, one of which irradiates the object, the ... mammalian cells, adherent cells, nonadherent cells, single cells and populations" said Mustafa Mir, a co-author of the paper. " ...
Clearly observing these specimens by bright field microscopy is difficult. ... Digital holographic microscopy (DHM) for phase-contrast imaging is employed for live cell imaging to study pathophysiology of ... Yu LF, Kim MK (2005) Wavelength-scanning digital interference holography for tomographic three-dimensional imaging by use of ... Cellular imaging using phase holographic microscopy: for the study of pathophysiology of red blood cells and human umbilical ...
Living mouse osteoblastic cells are quantitatively measured to demonstrate the capability and applicability of the system. ... and a deep learning-based holographic microscope using above neural network is implemented for quantitative phase imaging. ... and a deep learning-based holographic microscope using above neural network is implemented for quantitative phase imaging. ... Living mouse osteoblastic cells are quantitatively measured to demonstrate the capability and applicability of the system. ...
... a microfluidic chamber can be characterized by an interference microscopy configuration to obtain quantitative phase-contrast ... Cell (21) Cell Biochemistry and Biophysics (3) Cell Calcium (1) Cell Death and Disease (1) Cell Reports (6) Cellular and ... We demonstrate the use of holographic optical tweezers for trapping and manipulating silicon nanomembranes. These macroscopic ... We describe a method that advances live cell imaging by integrating an optical trap with spinning disk confocal microscopy with ...
In digital holographic beam shaping speckle occurrence is caused by interference of adjacent waves of a pixel-wise discretized ... contrast as well as high shape accuracy can be achieved by speckle-reduction of flat-top profiles with constrained phase. ... The AOS technology enables wavefront shaping [11] particularly for 3D nonlinear microscopy [12] and intensity profile shaping [ ... they lead to a lower electrical performance in manufacturing of solar cells [8]. The wear resistance of carbon-based coatings ...
"Digital holographic microscopy: A noninvasive contrast imaging technique allowing quantitative visualization of living cells ... The interference fringes due to the multiple reflections are not noticeable in the area where titanium is not deposited, but ... Precision phase-contrast imaging has been achieved with in-line phase-shifting digital holographic microscopy. The complex ... In this letter, very precise phase-contrast imaging using in-line phase-shifting digital holographic microscopy is reported. ...
"Autofocusing in digital holographic phase contrast microscopy on pure phase objects for live cell imaging." Applied Optics 47( ... "Enhanced quantitative phase imaging in self-interference digital holographic microscopy using an electrically focus tunable ... Modular digital holographic microscopy system for marker free quantitative phase contrast imaging of living cells. Progress in ... Determination of the integral refractive index of cells in suspension by digital holographic phase contrast microscopy. ...
... photo-activated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM)). In all cases, ... Smoother progression is facilitated by the use of comparable imaging modalities throughout the discovery and trial phases, ... In this paper, we will explore the techniques that have found success in aiding discovery in stem cell therapies and try to ... Microscopies and nanoscopies, especially those requiring fluorescent markers, have made an extraordinary impact on discovery at ...
Diffraction phase microscopy for quantifying cell structure and dynamics. Cuche et al. 1999. Simultaneous amplitude-contrast ... Digital holographic microscope for 3d imaging and process using it US20070070496A1 (en) 2007-03-29. Confocal self-interference ... For full discussion of the phase contrast method, see A. Bennett et al, Phase Microscopy (NY, J. Wiley & Sons, 1952).The ... Efficient phase defect detection system and method US8184298B2 (en) 2012-05-22. Spatial light interference microscopy and ...
Digital holographic microscopy. DIC. Digital interference phase contrast microscopy. DIHM. Digital in-line holographic ... Quantitative phase microscopy. QPI. Quantitative phase imaging. PS. Phase shifting. RBC. Red blood cell. ... Quantitative phase microscopy (QPM), a technique combining phase imaging and microscopy, enables visualization of the 3D ... Single-shot slightly off-axis digital holographic microscopy with add-on module based on beamsplitter cube J. A. Picazo-Bueno, ...
... noninterferometric quantitative phase microscopy (NIQPM), to perform measurements of total cell mass and subcellular density ... and Hilbert transform differential interference contrast microscopy (HTDIC) to determine volume. NIQPM is based on a simplified ... Basic Protocol, Issue 84, holography, digital inline holographic microscopy (DIHM), Microbiology, microscopy, 3D imaging, ... Bioengineering, Issue 86, Label-free optics, quantitative microscopy, cellular biophysics, cell mass, cell volume, cell density ...
SLIM is well suited for imaging single cells in both 2D and 3D, over many cell cycles, without photodamage. GLIM ... Quantitative Phase Imaging with SLIM and GLIM Fig.: ... Digital holography for quantitative phase-contrast imaging. Opt ... diffraction phase microscopy [30], spatial light interference microscopy (SLIM) [31], digital holographic microscopy [32], ... Spatial Light Interference Microscopy (SLIM) and Gradient Light Interference Microscopy (GLIM) are two QPI techniques that ...
... are traditionally viewed in a phase-contrast microscope or in a differential interference contrast microscope. These methods ... Digital holographic microscopy makes it possible to perform cell counting and to measure cell viability directly in the cell ... digital holography quantitative phase contrast microscopy Holographic interferometry holotomography holography microscopy ... Label-free cell cycle analysis. The phase shift induced by cells has been shown to be correlated to the cell dry mass. The cell ...
Quantitative phase imaging (QPI) is a novel label-free imaging technique bringing a completely new contrast into the live cell ... Q-PHASE uses a patented technology of coherence-controlled holographic microscopy to provide QPI of the highest quality. ... Strong suppression of coherent noise (speckles) & parasitic interferences (as opposed to laser-based approaches) ... Q-PHASE Brochure Q-PHASE - MULTIMODAL HOLOGRAPHIC MICROSCOPE - Quantitative phase imaging. Download the brochure! ...
"Single pixel quantitative phase imaging with spatial frequency projections, Methods" on DeepDyve, the largest online rental ... Spectral-domain optical coherence phase microscopy for quantitative phase-contrast imaging. Joo, C.; Akkin, T.; Cense, B.; Park ... Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells. Bon, P.; Maucort, G.; Wattellier ... Spatial light interference microscopy (slim). Wang, Z.; Millet, L.; Mir, M.; Ding, H.; Unarunotai, S.; Rogers, J.; Gillette, M. ...
The quantitative phase measurement apparatus ,b,1,/b, includes a reflective polarization splitting element ,b,17,/b,. The ... Provided is a small-sized quantitative phase measurement apparatus. ... Spatial light interference microscopy and fourier transform light scattering for cell and tissue characterization. 2009-11-26. ... In contrast, in the quantitative phase measurement apparatus 1 according to this embodiment, the distance between the principal ...
Image formation with phase objects. *Phase contrast according to Zernike. *Dark field microscopy and amplitude spatial filters ... multi-junction solar cell approach to increase the sunlight conversion efficiency, different solar cell architectures/li, ... Microscopy with holographic scan beams. *Lattice light-sheet microscopy. *Nearfield and Evanescent Field Microscopy *The ... Superposition of waves: Interference and Coherence. *Fourier-Optics. *Wave propagation and diffraction ...
Second, iterative phase retrieval algorithms can be applied to the holographic diffraction pattern to enhance the resolution to ... The intensities of the holographic interference pattern, which is reproduced in Figure 1 as a false color image was recorded ... A spatial resolution of about 50 nm is indicated by the width over which the contrast between black and white domains changes ... 1 the method can be extended by placing various samples like nanoparticles, colloids or cells into the object area. The shown ...
... with holographic (1-3) phase-contrast (4, 5) or differential interference contrast (DIC) (6) microscopy. Most of these ... Lensless high-resolution on-chip optofluidic microscopes for Caenorhabditis elegans and cell imaging. Proc. Natl. Acad. Sci. U. ... On-chip differential interference contrast microscopy using lensless digital holography. Opt. Express 18, 4717-4726 (2010).. ... Spiral phase contrast imaging in microscopy. Opt. Express 13, 689-694 (2005).. ...
  • Such objects do, however, induce a phase shift that can be observed using a phase contrast microscope. (wikipedia.org)
  • 7, 8 This makes it possible to upgrade a conventional light microscope with an add-on module to a real-time quantitative phase microscope. (spie.org)
  • Schematic of the electrically tunable lens (ETL)-based transport-of-intensity (TIE) equation quantitative phase microscope (TL-TIE). (spie.org)
  • Fig. 3 Quantitative phase image of a red blood cell smear obtained by means of a digital holographic microscope. (imperial.ac.uk)
  • Combination of the developed holographic methods with the methods of digital image processing allowed creating the digital holographic interference microscope (DHIM). (booksandbooks.com)
  • In this paper, an optimized structural convolution neural network PhaseNet is proposed for the reconstruction of digital holograms, and a deep learning-based holographic microscope using above neural network is implemented for quantitative phase imaging. (frontiersin.org)
  • Optical microscope is an effective diagnostic tool in modern healthcare which allows pathologists to clearly and qualitatively observe the details of cells and tissues, and make judgments based on experience. (frontiersin.org)
  • However, a bright field optical microscope records the intensity information of the specimen and suffers from low contrast for transparent biological cells which presents minimal light absorption. (frontiersin.org)
  • Transparent objects, like living biological cells, are traditionally viewed in a phase-contrast microscope or in a differential interference contrast microscope. (wikipedia.org)
  • The formation of an image in the microscope relies on the complex interplay between two critical optical phenomena: diffraction and interference. (microscopyu.com)
  • In the USSR, electron microscopy also is an independent scientific discipline whose subjects and objectives include the development of new electron microscopes and microscope accessories, the improvement of existing instruments, the development of techniques for preparing specimens investigated in electron microscopes, the study of formation mechanisms of electron-optical images, and the development of methods of analyzing the various types of information-not only images-obtained by means of electron microscopes. (thefreedictionary.com)
  • In Soviet practice, electron microscopy also deals with the development or improvement of other particle-beam microscopes, such as the proton microscope. (thefreedictionary.com)
  • Moreover, holographic imaging can be performed at a fraction of the size and cost of a conventional bright-field microscope, also covering a much larger field of view. (phys.org)
  • Despite these advantages, the resulting images of a holographic microscope in general suffer from light interference related spatial artifacts, which can limit the achievable contrast in the reconstructed hologram. (phys.org)
  • In Bright-field Holography, a deep neural network is trained using co-registered pairs of digitally refocused holograms and their corresponding bright-field microscope images to learn the statistical image transformation between two different microscopy modalities. (phys.org)
  • After its training, the deep neural network takes in a digitally refocused hologram corresponding to a given depth within the sample volume and transforms it into an image that is equivalent to a bright-field microscope image acquired at the same depth, matching the spatial and color contrast as well as the optical sectioning capability of a bright-field microscope. (phys.org)
  • Bright-field Holography bridges the contrast gap between the classical hologram reconstruction methods and a high-end bright-field microscope , while also eliminating the need to use complex hardware and mechanical scanning to rapidly image sample volumes. (phys.org)
  • Here, we demonstrate that a convolutional deep neural network can enhance optical microscopy images, without any hardware modification to the microscope. (aps.org)
  • 3 The FLUOVIEW FV3000 Series FV3000 and FV3000RS The Next Evolution of Confocal Laser Scanning Microscope Technology for Cell Biology, Cancer Research, Stem Cell Research, and Advanced Applications The FLUOVIEW FV3000 Series is designed to meet some of the most difficult challenges in modern science. (hobbydocbox.com)
  • The system features a modular, upgradable design that includes 2-tier detection options, easily upgradeable laser configurations, and the stable and flexible IX83 microscope with a field-upgradable z drift compensator (IX3-ZDC2) for fast and robust live cell autofocus. (hobbydocbox.com)
  • We firstly demonstrate the three-dimensional (3D) measurement of a nanometer-sized sphere held in optical tweezers in water using an in-line digital holographic microscope with a green light emitting diode. (blogspot.com)
  • Current applications for limited viewing angle three-dimensional tomographic reconstruction from a single viewpoint window are: measurement of fluid temperature using the CSLH (Confocal Scanning Laser Holography) microscope and detecting cancer cells using the ACHM (Acoustic Confocal Holography Microscope) ultra-sonic diagnostic instrument. (pbjacquemin.com)
  • The rate of particle uptake by rat alveolar macrophages (AM) exposed to zymosan (mean number of zymosan particles becoming bound to 100 cells at a fixed time interval) was determined with the aid of the scanning electron microscope (SEM). (faintpower.ga)
  • ON-CHIP PHASE MICROSCOPE/BEAM PROFILER BASED ON DIFFERENTIAL INTERFERENCE CONTRAST AND/OR SURFACE PLASMON ASSISTED INTERFERENCE - A differential interference contrast (DIC) determination device and method utilizes an illumination source, a layer having a pair of two apertures that receive illumination from the illumination source, and a photodetector to receive Young's interference from the illumination passing through the pair of two apertures. (patentsencyclopedia.com)
  • Quantum dots (Q-dots) label structures that can be observed in live cell mode by fluorescence microscopy. (imaging-git.com)
  • M. Sun & colleagues studied the behaviour of mitochondria during apoptosis using fluorescence microscopy in combination with 3D electron tomography of the same cells. (imaging-git.com)
  • Imaging of biological samples using fluorescence microscopy has advanced substantially with new technologies to overcome the resolution barrier of the diffraction of light allowing super-resolution of live samples. (jove.com)
  • Three-dimensional SIM (3D-SIM) is a wide-field fluorescence microscopy technique that offers a number of advantages over both single-molecule localization and STED. (jove.com)
  • This exposure problem could be completely avoided by using phase-contrast microscopy, implemented through the same optics as fluorescence microscopy. (google.com)
  • Functions for both phase-contrast and fluorescence autofocus were evaluated using the present invention and the suitability of phase-contrast autofocus for fluorescence microscopy was determined. (google.com)
  • 3i designs and manufactures technologies for living cell, live cell, and intravital fluorescence microscopy including digital holography, spinning disk confocal, multi-photon and lightsheet. (rms.org.uk)
  • Other closely related microscopy methods to digital holographic microscopy are interferometric microscopy, optical coherence tomography and diffraction phase microscopy. (wikipedia.org)
  • DHM is diffraction-limited by the numerical aperture, in the same way as traditional light microscopy. (wikipedia.org)
  • From such a holographic x-ray diffraction pattern the real space image can be retrieved by a single two-dimensional Fourier transformation. (stanford.edu)
  • The seemingly close relationship between diffraction and interference occurs because they are actually manifestations of the same physical process and produce ostensibly reciprocal effects. (microscopyu.com)
  • An interference pattern is created that is very similar to the one formed by double-slit diffraction (see Figure 17.8 and Figure 17.10 ). (texasgateway.org)
  • While the underlying thin layer of metal serves the purpose of suppressing the thermophoretic effect, the metal film step is found to induce a highly localized intensity distribution of light around the edge via self-interference of an incident wave and its boundary diffraction wave. (blogspot.com)
  • The photonic crystals of titanium dioxide and polymeric materials were characterized by scanning electron microscopy and optical diffraction measurements. (ox.ac.uk)
  • The microstructure, including microphase segregation and crystallinity, is quantitatively analyzed by using a combination of X-ray diffraction, grazing-incidence X-ray scattering, and atomic force microscopy. (anl.gov)
  • 23 Near Diffraction Limited Soft X-Ray Microscopy: 20 nm Spatial Resolution at 2.07 nm Wavelength W. Chao et al. (hobbydocbox.com)
  • Liquid crystalline materials with 5,5' -bis-(alkylpyridinyl)-2,2' -bithiophene structures with different positions of the nitrogen atoms in the aromatic core were synthesized, characterized by differential scanning calorimetry, polarized optical microscopy and, in some cases, by synchrotron X-ray diffraction analysis and single crystal X-ray analysis. (kent.edu)
  • This technology may enable acquisition of more information than is currently obtained through commonly used fluorescence modalities, such as confocal, multi-photon, and stimulated emission depletion microscopy imaging. (spie.org)
  • Scanning confocal microscopy has been used for this purpose, but the process of the three-dimensional scanning is rather time-consuming. (spiedigitallibrary.org)
  • Improving axial resolution in confocal microscopy with new high refractive index mounting media. (jove.com)
  • Resolution, high signal intensity and elevated signal to noise ratio (SNR) are key issues for biologists who aim at studying the localisation of biological structures at the cellular and subcellular levels using confocal microscopy. (jove.com)
  • Quantitative confocal spiral phase contrast. (sppin.fr)
  • Fluorescence confocal microscopy imaging was used to demonstrate the uptake of fluorescently labelled microspheres by cells and the ability to leverage their optical absorptivity in order to cause carbon graphitization and cell death. (blogspot.com)
  • The proposed method takes advantage of the precise optical manipulation by the laser microbeam and quantitative phase imaging by digital holographic microscopy with high spatial and temporal resolution. (osapublishing.org)
  • Quantitative phase contrast microscopy or quantitative phase imaging are the collective names for a group of microscopy methods that quantify the phase shift that occurs when light waves pass through a more optically dense object. (wikipedia.org)
  • Such systems enable real-time quantitative phase imaging with nanometer-scale and millisecond temporal resolution. (spie.org)
  • By use of this information, the whole complex amplitude distribution of the field can be recovered, enabling both quantitative phase imaging and numerical refocusing of the field to be done. (imperial.ac.uk)
  • In comparison, quantitative phase imaging techniques enable quantitative light field information and the visualization of transparent specimens [ 2 - 4 ]. (frontiersin.org)
  • Björn Kemper , Biomedical Technology Center, University of Muenster, "Digital Holographic Microscopy Enhances Cytometry and HistologyTechnology expands upon quantitative phase imaging, which can be integrated into microscopes commonly found in the laboratory. (uni-muenster.de)
  • Barroso, R. Radhakrishnan, S. Ketelhut, J. Schnekenburger, B. Kemper "Refractive index determination of buffer solutions from visible to near-infrared spectral range for multispectral quantitative phase imaging using a calibrated Abbe refractometer ", Proc. (uni-muenster.de)
  • J. Min, B. Yao, V. Trendafilova, S. Ketelhut, L. Kastl, B. Greve, B. Kemper,"Quantitative phase imaging of cells in a flow cytometry arrangement utilizing Michelson interferometer-based off-axis digital holographic microscopy", J. Biophoton. (uni-muenster.de)
  • B. Kemper, L. Pohl, M. Kaiser, J. Schnekenburger, and S. Ketelhut, "Quantitative phase imaging with digital holographic microscopy applied for label-free analysis of confluent cell layers," in Digital Holography and Three-Dimensional Imaging 2019, OSA Technical Digest (Optical Society of America, 2019), paper Th1A.4. (uni-muenster.de)
  • B. Kemper, L. Pohl, M. Kaiser, E. Doepker, J. Schnekenburger, S. Ketelhut, "Quantitative phase imaging-based concepts for the analysis of global morphology changes in confluent cell layers" Proc. (uni-muenster.de)
  • This enables label-free quantitative phase measurement (QPM), also called quantitative phase imaging (QPI), of living cells. (wikipedia.org)
  • Quantitative phase imaging (QPI) is a novel label-free imaging technique bringing a completely new contrast into the live cell imaging field. (tescan.com)
  • The quantitative phase imaging directly visualizes cellular mass (thickness × refractive index). (tescan.com)
  • Single pixel quantitative phase imaging is described with scattering theory to provide an intuitive understanding of the object spatial frequency information collected in both forward and back scattered geometries. (deepdyve.com)
  • Label-Free Classification of Cell Types by Imaging of Cell Membrane Fluctuations using Low Coherent Full-Field Quantitative Phase Microscopy", Proceedings of SPIE--New Developments in Microscopy, vol. 7500, 2010, San Francisco,California, USA. (patentgenius.com)
  • This has led to the development of non-invasive live cell imaging and automated cell culture analysis systems based on quantitative phase contrast microscopy. (wikipedia.org)
  • Imaging speeds as high as 800 cells/second in 3D were achieved by using HiLo wide-field depth resolved imaging and a remote z-scanning technique. (spie.org)
  • Such complete acquisition of scalar field (amplitude and phase) enables lens-free imaging, abberation compensation, and numerically focusing images at different depths. (spie.org)
  • It was originally derived by Teague from the Helmholtz equation under paraxial approximation more than 30 years ago, 3 when its main application fields were adaptive optics, electron microscopy, and x-ray imaging. (spie.org)
  • Recently, technological advancements in optical microscopy and digital signal processing have brought TIE back to the forefront of biomedical microscopic imaging. (spie.org)
  • We have successfully applied these systems for investigations of drug-induced morphology changes and phagocytosis of macrophages, 9 imaging of cellular dynamics of breast cancer cells, 10 and characterization of micro-optical elements. (spie.org)
  • Recently, we reformulated the TIE in the phase space to provide an elegant description of TIE phase-retrieval and computational imaging under partially coherent illumination. (spie.org)
  • 8 The phase-space formulation of the TIE further enables computational light-field imaging at full sensor resolution, without spatio-angular resolution trade-offs, which are often inevitable in conventional microlens array-based light-field imaging. (spie.org)
  • Deconvolved Spatial Light Interference Microscopy for Live Cell Imaging", IEEE Transactions on Biomedical Engineering, vol. 58, Issue 9, Sep. (patentgenius.com)
  • Label-Free High-Resolution Imaging of Live Cells With Deconvolved Spatial Light Interference Microscopy",Conference Proceedings IEEE Engineering in Medicine and Biology Society, pp. 3382-3385, 2010. (patentgenius.com)
  • 4. The optical system of claim 1, wherein the system is configured for label-free, high-contrast imaging of samples including one or more cells. (patentgenius.com)
  • Aiming to solve this problem, the combination of Brillouin and phase imaging systems is a promising assembly for separately monitoring the mechanical and optical properties of biological samples. (imperial.ac.uk)
  • Phase imaging techniques tackle the problem of measuring variations in the optical path length of light that passes through a given sample. (imperial.ac.uk)
  • There are mainly two groups of phase-imaging techniques: the first group, known as phase-contrast techniques, involves the acquisition of qualitative information of phase changes in the sample, which is typically related with the derivative of the phase. (imperial.ac.uk)
  • CHAMPAIGN, Ill., Aug. 29, 2011 - A new imaging method that can measure cell mass using two beams of light - called spatial light interference microscopy (SLIM) - offers new insights into whether cells grow at a constant rate or exponentially. (photonics.com)
  • Illinois researchers have developed a novel imaging technique, dubbed spatial light interference microscopy, that can quantitatively measure cell mass. (photonics.com)
  • Unlike most other cell-imaging techniques, SLIM - a combination of phase-contrast microscopy and holography - does not need staining or any other special preparation. (photonics.com)
  • Using this refractive-index imaging approach, we believe that we have detected an area in the cell that is attributed to the centriole. (sciencemag.org)
  • Today, clinicians base the process of sperm selection on morphology and dynamics assessment, which is carried out using two-dimensional (2-D) imaging methods, such as bright-field microscopy (BFM), differential interference contrast (DIC) microscopy, Hoffman's microscopy, and Zernike's phase-contrast microscopy ( 2 , 3 ). (sciencemag.org)
  • These imaging methods lack quantitative contrast, because of the prohibition of using exogenous contrast agents in human ICSI, and have distinctive imaging artifacts. (sciencemag.org)
  • Holographic imaging allows for a fully quantitative measurement of the cell optical thickness [i.e., the product of the refractive index (RI) and the physical thickness] ( 4 ). (sciencemag.org)
  • The book presents a clear and comprehensive review of the current status of the holographic microscopy with discussion of the positive and negative features of classical and holographic methods for solving the problem of three-dimesional (3D) imaging of phase microscopic objects. (booksandbooks.com)
  • Digital holographic microscopy (DHM) for phase-contrast imaging is employed for live cell imaging to study pathophysiology of red blood cells (RBCs) and human umbilical vein endothelial cells (HUVECs). (springerprofessional.de)
  • Marquet P, Rappaz B, Magistretti PJ, Cuche E, Emery Y, Colomb T et al (2005) Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy. (springerprofessional.de)
  • As a typical representative of this technique, digital holographic microscopy (DHM) can be implemented for complex amplitude imaging and be used to investigate transparent specimens, such as biological samples including tissues, dry mass, membrane fluctuation, etc [ 5 - 8 ]. (frontiersin.org)
  • The label-free, submicron scale sensitivity, full-field, non-destructive, real-time, quantitative and three-dimensional imaging abilities of DHM present a variety of advantages for biomedical applications, especially for live cell imaging [ 14 - 16 ]. (frontiersin.org)
  • In our previous work, a common-path digital holographic microscopy based on a beam displacer unit was proposed for quantitative and dynamic phase imaging of biological cells [ 17 ]. (frontiersin.org)
  • A. Vollmer, Book Chapter "Label-free quantitative in-vitro live cell imaging with digital holographic microscopy", in Bioanalytical Reviews, Ed.: J. Wegener (Springer Publishing, 2019). (uni-muenster.de)
  • L. Tacke, K. Brinker, and B. Kemper, "Digital Filtering Approaches for Noise Reduction in Quantitative Digital Holographic Phase Contrast Imaging of Living Cells," in Digital Holography and Three-Dimensional Imaging 2019, OSA Technical Digest (Optical Society of America, 2019), paper Th3A.38. (uni-muenster.de)
  • Precision phase-contrast imaging has been achieved with in-line phase-shifting digital holographic microscopy. (spiedigitallibrary.org)
  • Quantitative phase microscopy (QPM), a technique combining phase imaging and microscopy, enables visualization of the 3D topography in reflective samples, as well as the inner structure or refractive index distribution of transparent and translucent samples. (osapublishing.org)
  • With the drive to translate stem cell therapies into pre-clinical and clinical trials, early selection of the right imaging techniques is paramount to success. (biomedcentral.com)
  • There are many instances in regenerative medicine where the biological, biochemical, and biomechanical mechanisms behind the proposed function of stem cell therapies can be elucidated by appropriate imaging. (biomedcentral.com)
  • Microscopies and nanoscopies, especially those requiring fluorescent markers, have made an extraordinary impact on discovery at the molecular and cellular level, but due to their very limited ability to focus in the scattering tissues encountered for in vivo applications they are largely confined to superficial imaging applications in research laboratories. (biomedcentral.com)
  • Smoother progression is facilitated by the use of comparable imaging modalities throughout the discovery and trial phases, giving label-free techniques an advantage wherever they can be used, although this is seldom considered in the early stages. (biomedcentral.com)
  • Hence we try to provide, in this paper, a means to compare the best known imaging technologies in terms of their capabilities and limitations for stem cell research. (biomedcentral.com)
  • Table 1 provides an overview of the optimal stem cell tracking characteristics, the probes used to achieve this, and the appropriate imaging modalities with their advantages and disadvantages. (biomedcentral.com)
  • Resolution is improved, with typical lateral and axial resolutions of 110 and 280 nm, respectively and depth of sampling of up to 30 µm from the coverslip, allowing for imaging of whole cells. (jove.com)
  • Fig.: SLIM is well suited for imaging single cells in both 2D and 3D, over many cell cycles, without photodamage. (imaging-git.com)
  • Gradient Light Interference Microscopy (GLIM) is a new QPI technique that provides label-free and quantitative 3D tomographic imaging of tissues, organoids, embryos, model multicellular organisms with high resolution and remarkable contrast. (imaging-git.com)
  • An important challenge in imaging thick 3D biostructures with any optical method is loss of contrast due to multiple scattering [11]. (imaging-git.com)
  • It provides information that can be interpreted into many underlying biological processes as explained in the section "Living cells imaging" below. (wikipedia.org)
  • QPI images offer the highest cell/background contrast among other label-free imaging techniques thus enabling the most reliable and precise automatic segmentation of individual cells. (tescan.com)
  • We demonstrate a method of common-path single pixel quantitative phase microscopy that opens the ability for phase imaging in new spectral regions, such as short wave infrared and mid infrared. (deepdyve.com)
  • Simultaneous imaging of quantitative phase, absorption, and fluorescence through spatial frequency projections recorded on two separate detectors. (deepdyve.com)
  • Photoacoustic tomography is an emerging biomedical imaging technique that combines optical contrast with ultrasound resolution. (uwm.edu)
  • Bright-field Holography (BH): a novel technique for high contrast microscopic object imaging in sample volumes. (phys.org)
  • Digital holographic microscopy is an imaging modality that can digitally reconstruct the images of 3-D samples from a single hologram by digitally refocusing it through the entire 3-D sample volume. (phys.org)
  • Researchers at UCLA have developed a novel artificial neural network -based method to overcome these limitations of holographic 3-D imaging. (phys.org)
  • This new method, termed Bright-field Holography, has the best of both worlds as it combines the image contrast advantage of bright-field microscopy and the snapshot volumetric imaging capability of holography. (phys.org)
  • Yichen Wu et al, Bright-field holography: cross-modality deep learning enables snapshot 3D imaging with bright-field contrast using a single hologram, Light: Science & Applications (2019). (phys.org)
  • Trendsetting in materials microscopy, circular polarization with DIC solves a familiar imaging problem. (zeiss.com)
  • 3. F. Tiaho, G. Recher, and D. Rouède, Estimation of helical angles of myosin and collagen by second harmonic generation imaging microscopy, Opt. (docplayer.net)
  • 9. G. Latour, I. Gusachenko, L. Kowalczuk, I. Lamarre, and M. C. Schanne-Klein, In vivo structural imaging of the cornea by polarization-resolved second harmonic microscopy, Biomed. (docplayer.net)
  • Efficient bioluminescence resonance energy transfer (BRET) from a bioluminescent protein to a fluorescent protein with high fluorescent quantum yield has been utilized to enhance luminescence intensity, allowing single-cell imaging in near real time without external light illumination. (spie.org)
  • Moreover, BRAC signals were acquired by ratiometric imaging capable of canceling out Ca 2+ -independent signal drifts due to change in cell shape, focus shift, etc. (spie.org)
  • The work extends previous x-ray imaging of biological cells in the simple in-line holography geometry to full three-dimensional reconstruction, based on a fast iterative phase reconstruction algorithm which circumvents the usual twin-image problem. (springeropen.com)
  • Optical nanoscopy based on visible light fluorescence is becoming an important tool for three-dimensional imaging of biological cells at the nanoscale. (springeropen.com)
  • However, for biological samples the applied dose is a crucial parameter in view of structural changes and radiation damage during the imaging process, inhibiting live cell imaging and creating a need for fixation. (springeropen.com)
  • With the high sensitivity and speed required for live cell and tissue imaging and the ease of use and flexibility required for microplate imaging and complex screening protocols, the FV3000 Series supports complete workflows from live cell 2D 6D (x,y,λ,z,t,p) imaging through image processing, like deconvolution, and analysis. (hobbydocbox.com)
  • The FV3000 is optimized for macro to micro imaging of cells, tissues, and small organisms. (hobbydocbox.com)
  • The optical path enables macro to micro imaging from 1.25X to 150X magnification combined with robust, intuitive automation to simplify complex experiments, including one-click cellsens macro analysis for cell counting and segmentation analysis. (hobbydocbox.com)
  • 1) (2) (3) (4) Microfluidics and High-Speed Blood Flow Circulating tumor cells in peripheral blood and microfluidic device imaging can require high-speed imaging for accurate measurements. (hobbydocbox.com)
  • Olympus exclusive silicone objectives provide clear and bright images at depth in live cells and tissues for accurate imaging and quantification. (hobbydocbox.com)
  • UPLSAPO60XS2 (NA 1.3, W.D. 0.3 mm, silicone oil ne = 1.4) 5 μm 5 μm 35 μm 35 μm UPLSAPO 60XO (NA 1.35, W.D mm, immersion oil ne = 1.52) (5) (6) Macro to Micro and Whole Slide Imaging Cell biology research demands the flexibility to image small organisms at the macro scale down to the micro at high resolution. (hobbydocbox.com)
  • 5 Spheroid, Gel Matrix, Long-Term Time-Lapse, and Microplate Imaging Long-term time-lapse imaging of live cells in 3D captures physiologically relevant information. (hobbydocbox.com)
  • As stem cells grow into spheroids and organoids, the FV3000 Series enables precise, stable time-lapse imaging with high sensitivity and low phototoxicity. (hobbydocbox.com)
  • Key amongst these technologies is the new technique Infrared Photo-induced Force Microscopy (IR PiFM) which delivers robust, easy and superfast Nanoscale Chemical Imaging & Spectroscopy. (rms.org.uk)
  • In addition to the changes in spectroscopic signal and contrast, near-field optical imaging of nanoobjects is also affected by strong plasmonic interactions between the tip and the sample. (umk.pl)
  • Digital holographic (DH) microscopy is a digital high-resolution holographic imaging technique with the capacity of quantification of cellular conditions without any staining or labeling of cells. (intechopen.com)
  • The technique is cell friendly, fast and simple to use and has unique imaging capabilities for time-lapse investigations on both the single cell and the cell-population levels. (intechopen.com)
  • X-ray phase-contrast imaging (X-PCI) offers increased sensitivity with respect to standard radiography and CT allowing an enhanced visibility of adjoining, low density structures with an almost histological image resolution. (biomedcentral.com)
  • While morphological MRI is limited by satisfactory spatial and contrast resolutions, quantitative MRI techniques for analyzing cartilage matrix composition (collagen/proteoglycan content), such as delayed gadolinium-enhanced MRI of cartilage, sodium imaging [ 10 ] or T2 relaxation time mapping [ 11 ], have not been established in routine examinations because of their time-consuming acquisition, post-processing and resultant low resolution map of matrix changes. (biomedcentral.com)
  • COHERENT ANTI-STOKES RAMAN HOLOGRAPHY - Apparatus and methods of four wave mixing (FWM) holography are described, including illuminating a sample with a first beam, a second beam, and a third beam, and combining the generated FWM signal with a reference beam at a imaging device to obtain holographic image data. (patentsencyclopedia.com)
  • Cell viability assays and brightfield imaging after one week confirm cell survival and that the patterns persist. (mpg.de)
  • Single-molecule localization microscopy such as PALM, STORM, GSDIM, etc allow to get super-resolved images of cell ultrastructure by precise localization of individual fluorescent molecules via their temporal isolation. (spie.org)
  • There are currently three main types of super-resolution techniques - stimulated emission depletion (STED), single-molecule localization microscopy (including techniques such as PALM, STORM, and GDSIM), and structured illumination microscopy (SIM). (jove.com)
  • Conventional phase contrast microscopy and related methods, such as differential interference contrast microscopy, visualize phase shifts by transforming phase shift gradients into intensity variations. (wikipedia.org)
  • Although phase contrast microscopy or differential interference contrast microscopy, which converts the sightless phase shifts introduced by the specimen of interest into observable intensity variations, provide an approach to survey phase specimens without labeling, they cannot provide quantitative phase information on the specimen-induced phase shifts for subsequent accurate diagnosis. (frontiersin.org)
  • We present a new acquisition method that enables high-resolution, fine-detail full reconstruction of the three-dimensional movement and structure of individual human sperm cells swimming freely. (sciencemag.org)
  • The reconstruction is based solely on the natural movement of the sperm cell and a novel set of algorithms, enabling the detailed four-dimensional recovery. (sciencemag.org)
  • Cuche E, Marquet P, Depeursinge C (1999) Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms. (springerprofessional.de)
  • In this handheld device, we also employ an iterative phase retrieval-based image reconstruction method, which offers the ability to digitally image a highly multiplexed array of sensors on the same plasmonic chip, making this approach especially suitable for high-throughput diagnostic applications in field settings. (nature.com)
  • Moreover, radiation damage of samples is considered to be the ultimate limitation in x-ray microscopy, if resolution due to the characteristics of sources, optical components, reconstruction algorithms and detection is successfully scaled down (Howells et al. (springeropen.com)
  • Here we present a 3D tomographic reconstruction of unstained freeze-dried cells of the gram-positive bacterium Deinococcus radiodurans using hard x-rays (13.8 keV photon energy). (springeropen.com)
  • 2012 ). Despite the low dose, phase reconstruction based on recorded holographic intensities was not hampered by noise, primarily due to two reasons: (i) the use of a highly coherent beam with curved wavefronts (Salditt et al. (springeropen.com)
  • Here, we demonstrate that a deep convolutional neural network can achieve phase retrieval and holographic image reconstruction, using a single intensity-only hologram of a complex sample, which conventionally required multiple measurements for phase recovery. (aps.org)
  • Using a trained convolutional neural network, phase recovery and holographic image reconstruction of a complex-valued sample are performed in a single feed-forward pass, considerably reducing the measurement and the computation time when compared to measurement diversity based phase recovery approaches. (aps.org)
  • Holography was invented by Dennis Gabor to improve electron microscopy. (wikipedia.org)
  • Most of the currently available information on structures of macromolecules and proteins has been obtained from either X-ray crystallography experiments or cryo-electron microscopy investigations by means of averaging over many molecules assembled into a crystal or over a large ensemble selected from low signal-to-noise ratio electron micrographs, respectively ( 1 ). (pnas.org)
  • The objects investigated in electron microscopy are mainly solids. (thefreedictionary.com)
  • In addition to the investigation of static objects, which do not vary in time, electron microscopy makes it possible to study various processes in their dynamical evolution. (thefreedictionary.com)
  • In this article, off-axis electron holography, a transmission electron microscopy (TEM) technique that yields a vector map of the magnetic field in a sample with nanometer resolution ( 6-8 ), is used to image the magnetic microstructure of a natural titanomagnetite intergrowth. (pnas.org)
  • 2011 ). The electron density distribution and an effective mass density distribution of the cellular structure was extracted quantitatively from the reconstructed 3D phase information. (springeropen.com)
  • While studying tissues using electron microscopy, John Foxton Ross Kerr at University of Queensland was able to distinguish apoptosis from traumatic cell death. (wikipedia.org)
  • This study examined the feasibility of X-PCI for high-resolution (sub-) micrometer analysis of different stages in tissue degeneration of human cartilage samples and compare it to histology and transmission electron microscopy. (biomedcentral.com)
  • For correlation, osteochondral samples were processed for histology and transmission electron microscopy. (biomedcentral.com)
  • X-PCI provides a three-dimensional visualization of healthy and moderately degenerated cartilage samples down to a (sub-)cellular level with good correlation to histologic and transmission electron microscopy images. (biomedcentral.com)
  • Mann CJ, Yu LF, Lo CM, Kim MK (2005) High-resolution quantitative phase-contrast microscopy by digital holography. (springerprofessional.de)
  • Therefore, an in-line phase-shifting digital holography system can be more useful in the precision shape measurement of transparent objects whose thickness or height profiles are proportional to the phase profiles of their complex images. (spiedigitallibrary.org)
  • Digital holographic microscopy (DHM) is digital holography applied to microscopy. (wikipedia.org)
  • Digital holography comes in different flavors, such as off-axis Fresnel, Fourier, image plane, in-line, Gabor and phase-shifting digital holography, depending on the optical setup. (wikipedia.org)
  • From the recorded interference pattern, the intensity and the phase shift image is numerically created by a computer algorithm. (wikipedia.org)
  • v Abstract Thin Film Dielectric Properties Characterization By Scanning Near-Field Microwave Microscopy We present a method for extracting high-spatial resolution dielectric constant data at microwave frequencies. (docme.ru)
  • It is possible to precisely quantify the phase of optically transparent biological cells and tissues using digital holographic microscopy. (spie.org)
  • Light propagation within an optically thick biological specimen (e.g. tissues) depends on the scattering and absorption properties of its components: cells, cell organelles, and various fiber structures. (imaging-git.com)
  • Recent studies using dielectric spectroscopy have reported on the structures of numerous types of cell suspensions and tissues, and also time-dependent processes involving them, such as cell sedimentation, cell aggregation, cell division, and tissue viability after excision. (uwm.edu)
  • The mass density distribution within native unstained and unsliced biological cells and tissues is such a case in point. (springeropen.com)
  • This work demonstrates that organic transistors based on organic blends are excellent bioelectronics transducer for extracellular electrical recording of excitable cells and tissues thus providing a valid alternative to electrochemical transistors. (ibecbarcelona.eu)
  • Expression of IL-10 in human normal and cancerous ovarian tissues and cells. (faintpower.ga)
  • However, arbitrary, non-symmetrically shaped cell assemblies are necessary to obtain the irregular cellular arrangements found in biological tissues. (mpg.de)
  • One of the most suitable techniques for performing this task is digital holographic microscopy, which only requires recording the interference between a beam diffracted by the sample and a free-space propagated beam. (imperial.ac.uk)
  • In the holographic process, a coherent beam first is split into two component beams, one of which irradiates the object, the second of which irradiates a recording medium. (photonics.com)
  • Both methods enable flexible holographic beam shaping due to the local variation of the phase of the laser beam. (springer.com)
  • LCoS-SLM is based on the individual manipulation of the refractive index of pixels of a liquid crystal display, while acousto-optic beam shaping (AOS) is based on a fast variation of an acoustic wave enabling a spatially dependent phase variation of laser beams. (springer.com)
  • One major issue of holographic laser beam shaping is given by occurrence of speckle in the image plane of focusing optics which significantly reduces flat-top quality by strongly appearing intensity fluctuations. (springer.com)
  • The well-known "phase problem of x-ray scattering" which is the intrinsic loss of the scattering phases in the recorded intensities is overcome by interfering the beam from the sample with a reference beam through a tiny pinhole. (stanford.edu)
  • Third, the interference between sample and reference beam breaks up the scattering intensities into individual speckles by superimposing a high-frequency oscillation that causes a stripe-like appearance of the scattering pattern. (stanford.edu)
  • The path difference, the difference in the distance traveled by each beam, creates a phase difference between them. (wikipedia.org)
  • : 14-17 If a single beam has been split along two paths, then the phase difference is diagnostic of anything that changes the phase along the paths. (wikipedia.org)
  • The fringes can be interpreted as the result of interference between light coming from the two virtual images S ′ 1 and S ′ 2 of the original source S . The characteristics of the interference pattern depend on the nature of the light source and the precise orientation of the mirrors and beam splitter. (wikipedia.org)
  • A holographic image is produced by constructive and destructive interference of a split laser beam. (texasgateway.org)
  • Microstructuring of large scale 3D scaffolds is investigated and the direct laser writing technique is supplemented by fabrication by multi-beam interference and micromolding of large scale structures. (spie.org)
  • In our group, an optical super-resolution microscopy system, based on Bessel Beam has been developed. (aps.org)
  • When a vortex beam with the correct number of coils per wavelength strikes the gold plating on the detector's surface, it encounters a holographic interference pattern that has been etched into the gold. (phys.org)
  • If the incoming light doesn't match the interference pattern, the plasmon beam fails to focus or converge and is blocked from reaching the detector. (phys.org)
  • More than just detecting a specific twisted beam, our detectors gather additional information on the phase of the light beam. (phys.org)
  • Using the same holographic approach, the same device patterned in different ways should be able to couple any type of free-space light beam into any type of surface wave," says Genevet. (phys.org)
  • Quantitative phase contrast methods are distinguished from conventional phase contrast methods in that they create a second so-called phase shift image or phase image, independent of the intensity (bright field) image. (wikipedia.org)
  • Phase unwrapping methods are generally applied to the phase shift image to give absolute phase shift values in each pixel, as exemplified by Figure 1. (wikipedia.org)
  • The principal methods for measuring and visualizing phase shifts include ptychography and various types of holographic microscopy methods such as digital holographic microscopy, holographic interference microscopy and digital in-line holographic microscopy. (wikipedia.org)
  • Common to these methods is that an interference pattern (hologram) is recorded by a digital image sensor. (wikipedia.org)
  • Arbitrary images can be projected with amplitude-only calibration (no phase measurement) and fidelities on par with conventional full-measurement methods. (spiedigitallibrary.org)
  • 1 Historically, visualization of phase has been achieved non-quantitatively, with contrast methods such as Zernike phase contrast and differential interference contrast (DIC). (spie.org)
  • To this end, we have been working on the development of specialized computational methods for the extraction of quantitative information (for phase and beyond) non-interferometrically, by solving the so-called transport-of-intensity equation (TIE). (spie.org)
  • This is without the need for phase unwrapping (removing discontinuities in the extracted phase), as is common for many interferometric methods. (spie.org)
  • Systems and methods described herein employ multiple phase-contrast images with various relative phase shifts between light diffracted by a sample and light not diffracted by the sample to produce a quantitative phase image. (patentgenius.com)
  • A significant advantage over existing methods is that we can measure all types of cells - bacteria, mammalian cells, adherent cells, nonadherent cells, single cells and populations" said Mustafa Mir, a co-author of the paper. (photonics.com)
  • Most commonly, four-dimensional (4-D) tracking methods for sperm cells have been suggested on the basis of pinpointing the 3-D location of a single point on the sperm, usually the sperm head centroid, over time ( 4 , 8 - 11 ). (sciencemag.org)
  • Classical and holographic methods of phase, interference and polarization contrast are discussed. (booksandbooks.com)
  • Digital holographic microscopy distinguishes itself from other microscopy methods by not recording the projected image of the object. (wikipedia.org)
  • Common to all methods is the use of a reference wave front to obtain amplitude (intensity) and phase information. (wikipedia.org)
  • These methods visualize phase shifting transparent objects by distorting the bright field image with phase shift information. (wikipedia.org)
  • Also, methods to tackle the effect of optical distortions on this microscopy system, using Adaptive Optics will be discussed, which can further improve the resolution as well as photon efficiency. (aps.org)
  • We achieve both retrieval of the three-dimensional refractive-index profile of the sperm head, revealing its fine internal organelles and time-varying orientation, and the detailed four-dimensional localization of the thin, highly-dynamic flagellum of the sperm cell. (sciencemag.org)
  • Park YK, Diez-Silva M, Popescu G, Lykotrafitis G, Choi WS, Feld MS et al (2008) Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum. (springerprofessional.de)
  • M. Baczewska, K. Eder, S. Ketelhut, B. Kemper , M. Kujawińska, "Holotomographic investigation of an influence of PFA cell fixation process on refractive index of cellular organelles in epithelial cells," Proc. (uni-muenster.de)
  • P. Heiduschka, G. Nettels-Hackert, J. Schnekenburger, B. Kemper "Morphology and spatial refractive index distribution of the retina accessed by hyperspectral quantitative phase microscopy", Proc. (uni-muenster.de)
  • Barroso, S. Ketelhut, P. Heiduschka, G. Nettels-Hackert, J. Schnekenburger and B.Kemper "Refractive index properties of the retina accessed by multi-wavelength digital holographic microscopy", Proc. (uni-muenster.de)
  • In transparent objects, these mechanisms are often too weak, and interference effects are more suitable to observe the tiny refractive index variations that produce phase shifts. (sciencemag.org)
  • With this technique we have made microperiodic polymeric structures, and we have used these as templates to create complementary structures with higher refractive-index contrast. (ox.ac.uk)
  • The main optical parameters, such as the shape of illumination and the numerical aperture of the objective, together with the noise of the camera used to determine the minimum phase that can be detected. (spiedigitallibrary.org)
  • By controlling the scanning pattern in multi-photon microscopy and applying appropriate numerical algorithms, we developed a striped-illumination approach, which enabled us to achieve 3-fold better axial resolution and improved signal-to-noise ratio, i.e. contrast, in more than 100 µm tissue depth within highly scattering tissue of lymphoid organs as compared to standard multi-photon microscopy. (jove.com)
  • By using the striped-illumination approach, we are able to observe the dynamics of immune complex deposits on secondary follicular dendritic cells - on the level of a few protein molecules in germinal centers. (jove.com)
  • To create the necessary interference pattern, i.e., the hologram, the illumination needs to be a coherent (monochromatic) light source, a laser for example. (wikipedia.org)
  • This invention is also for a method and apparatus to direct the lateral motion and induce the assembly into planar arrays of cells on semiconductor surfaces in response to temporally and spatially varying electric fields and to projected patterns of illumination. (google.es)
  • 16 A Novel Illuminator is Required Scanning optics modify phase space, transforming elliptical spatial distribution to circular, and increasing the angular illumination to provide the desired degree of partial coherence. (hobbydocbox.com)
  • Refining Common Path Interferometry with a Spiral Phase Fourier filter", Journal of Optics A:Pure and Applied Optics, 11 094023, 2009. (patentgenius.com)
  • Interferometry is a family of techniques in which waves, usually electromagnetic waves , are superimposed , causing the phenomenon of interference , which is used to extract information. (wikipedia.org)
  • Digital holographic microscopy enables the measurement of the quantitative light field information and the visualization of transparent specimens. (frontiersin.org)
  • The powerful Q-PHASE analysis toolbox processes segmented images on-the-fly and provides a complex portfolio of tools for data visualization, subpopulation gating and multi-parameter data mining. (tescan.com)
  • With this BRAC, we succeeded visualization of Ca 2+ dynamics at the single-cell level with temporal resolution at 1 Hz. (spie.org)
  • A combination of optics coding and digital processing enhances the capabilities of traditional light microscopes, enabling acquisition of information such as phase, which cannot otherwise be captured. (spie.org)
  • Label-Free Intracellular Transport Measured by Spatial Light Interference Microscopy", Journal of Biomedical Optics,vol. 16, Issue 2, Feb. 2011. (patentgenius.com)
  • This phase moiré effect and the classical moiré effect from opaque lines are two ends of a continuous spectrum in optics, which is called the universal moiré effect. (wikipedia.org)
  • He realizado un total de dos años de estancias en otros centros de investigación, incluyendo entre otros: la Universidad de Purdue (Estados Unidos), la FernUnivesitat en Hagen (Alemania) y el Institute of Applied Optics (Polonia). (uji.es)
  • Three components contribute to our vision capabilities: the optics of the eye, the light detection by the photoreceptor cells (PRCs), and the processing of the visual information in the neuronal structures of the retina and the brain. (springer.com)
  • Fig. 1 Numerical refocusing of the amplitude of a test target USAF 1951 by means of digital holographic microscopy: (a) Reconstructed intensity in the camera plane. (imperial.ac.uk)
  • In DHM, a hologram that carries specimen information is recorded digitally first, and then the hologram is numerically reconstructed to extract the amplitude or phase of the specimens' complex field [ 9 - 13 ]. (frontiersin.org)
  • The complex amplitude of the object field on the charge-coupled device plane is measured by the phase-shifting method with a self-calibration algorithm, and the magnified object image is reconstructed with a plane wave expansion method. (spiedigitallibrary.org)
  • We show the distortion of plasmon patterns in nanoscale optical microscopy of metallic objects, such as gold nanodisks and dimers when the tip interacts strongly with the objects, modifying the near-field plasmon patterns both in amplitude and phase. (umk.pl)
  • Within milliseconds, FF-SS-OCT acquires three-dimensional images of the retina which contain information on amplitude and phase of the scattered light virtually free of motion artifacts. (springer.com)
  • Here, we report a quantitative phase laser microsurgery system for evaluation of cellular/sub-cellular dynamic changes during laser micro-dissection. (osapublishing.org)
  • The latter paves the way to use RBCs as model cells to investigate molecular regulation of cellular adhesion processes. (blogspot.com)
  • Monitoring cellular communication by intravital deep-tissue multi-photon microscopy is the key for understanding the fate of immune cells within thick tissue samples and organs in health and disease. (jove.com)
  • It is directly proportional to the cellular dry mass and can be used for monitoring and direct quantification of dry mass changes inside the cells in pg/μm 2 . (tescan.com)
  • Recently, DH microscopy has been used for cellular diagnosis in the clinic, providing support for using the concept of DH, e.g., screening of malaria infection of red blood cells (RBC), cervix cancer screening, and sperm quality. (intechopen.com)
  • Various cellular parameters can be visualized and calculated from the particular hologram, including individual cell area, thickness, volume, and population confluence and cell counts [ 4 - 10 ]. (intechopen.com)
  • In contrast to necrosis , which is a form of traumatic cell death that results from acute cellular injury, apoptosis is a highly regulated and controlled process that confers advantages during an organism's lifecycle. (wikipedia.org)
  • Without ergosterol, 14 alpha-methyl sterols increase which subsequently causes the fungal cell membrane to exhibit increased cellular permeability. (statescale.ga)
  • Acoustophoretic cell manipulation by holographic fields thus holds promise for non-contact, long-range, long-term cellular pattern formation, with a wide variety of potential applications in tissue engineering and mechanobiology. (mpg.de)
  • Specifically, TIE links the phase to variations in intensity induced by wave propagation, which enables retrieval of the phase simply by measuring the intensity along the propagation direction at multiple positions. (spie.org)
  • Using an optimum finite difference scheme to estimate an intensity derivative, 4 and combining this with the boundary-artifacts-free TIE solver based on a fast discrete cosine transform, 5, 6 we can directly and accurately reconstruct the continuous phase of the optical field. (spie.org)
  • In contrast, a Gaussian shaped intensity distribution can be disadvantageous in several micromachining applications. (springer.com)
  • In traditional microscopy, which do not use a reference wave front, only intensity information is recorded and essential information about the object is lost. (wikipedia.org)
  • A general theory for single pixel intensity and quantitative phase microscopy is presented. (deepdyve.com)
  • This encodes the scattering phases in additional intensity modulations. (stanford.edu)
  • They may either interfere constructively (strengthening in intensity) if their light waves arrive in phase, or interfere destructively (weakening in intensity) if they arrive out of phase, depending on the exact distances between the three mirrors. (wikipedia.org)
  • This works because when two waves with the same frequency combine, the resulting intensity pattern is determined by the phase difference between the two waves-waves that are in phase will undergo constructive interference while waves that are out of phase will undergo destructive interference. (wikipedia.org)
  • Waves which are not completely in phase nor completely out of phase will have an intermediate intensity pattern, which can be used to determine their relative phase difference. (wikipedia.org)
  • The phase sensitivity limit of Differential Phase Contrast (DPC) with partially coherent light is analyzed in details. (spiedigitallibrary.org)
  • We propose a simple algorithm to predict phase sensitivity of a DPC setup, which can be performed before the setup is built. (spiedigitallibrary.org)
  • Because of SLIM's sensitivity, the researchers could monitor cells' growth through different phases of the cell cycle. (photonics.com)
  • We and others have used DH microscopy showing that the technique has the sensitivity to distinguish between different cells and treatments. (intechopen.com)
  • In contrast to the current state of the art in structural biology, we do away with the need for averaging over many molecules. (pnas.org)
  • This effect is calculated and addressed both for the far-field signal of ODT molecules on gold nanowires, as well as for the near-field infrared signal of a molecular thin layer of PMMA on a resonant cavity in scattering-type near-field scanning optical microscopy (s-SNOM). (umk.pl)
  • Among the most significant drawbacks, a selective delivery of the editing molecules to the target cells, markedly affecting the editing efficiency must be emphasized. (vutbr.cz)
  • Hence, the aim of this PhD project will be the use of variously modified hybrid organic/inorganic nanoparticles either static or dynamic as biocompatible delivery tools for gene editing molecules to increase the tissue/cell specificity and editing efficiency. (vutbr.cz)
  • The second group allows retrieving the quantitative information of the phase (by means of a single-shot in some cases) and are based on interferometric-holographic acquisition of the field scattered by the sample. (imperial.ac.uk)
  • 1 nm) achieved using a novel phase-shifting interferometric operation makes the proposed device ideal for examining transparent substrates and reading microarrays of biomarkers. (sciencemag.org)
  • Interferometric lithography (IL) uses the interference of laser light to produce nanostructures over large areas via a simple process implemented under ambient conditions. (rsc.org)
  • Furthermore, the complete scalar field acquisition provides tremendous flexibility to realize various microscopy modalities computationally, such as dark field, Zernike phase contrast, and DIC, without requiring specialized hardware components. (spie.org)
  • The PDLC material offers all of the features of holographic photopolymers with the added advantage that the hologram can be switched on and off with the application of an electric field. (google.co.uk)
  • Upon irradiation, the liquid crystal separates as a distinct phase of nanometer-size droplets aligned in periodic channels forming the hologram. (google.co.uk)
  • We show that arbitrarily shaped cell patterns can be obtained from the complex acoustic field distribution defined by an acoustic hologram. (mpg.de)
  • Recently, an intense research effort has been devoted to developing lens-free microscopes (LFMs), which work in a scattering or holographic configuration and can offer unprecedented large DOF and FOV by using unfocused light beams and the full photosensitive area of a complementary metal-oxide semiconductor (CMOS) or a charge-coupled camera ( 7 - 15 ). (sciencemag.org)
  • Investigations on Label-Free Identification of Subcellular Tumor Cell Structures by Digital Holographic Phase Contrast Microscopy",DGaO-Proceedings, 2009. (patentgenius.com)
  • Tissue-like structures called organoids [7-9] are used to recapitulate the phenotypes, function, and regulation of signaling pathways for cells in in-vivo tumors [10]. (imaging-git.com)
  • Here we describe a technique--three-dimensional holographic lithography--that is well suited to the production of three-dimensional structures with sub-micrometre periodicity. (ox.ac.uk)
  • The fabrication of three-dimensional photonic crystal structures with sub-micron periodicity was performed by holographic lithography. (ox.ac.uk)
  • X-PCI is able to resolve the three layers and the architectural organization of cartilage including changes in chondrocyte cell morphology, chondrocyte subgroup distribution and (re-)organization as well as its subtle matrix structures. (biomedcentral.com)
  • The Bergamo ® II Series is Thorlabs' platform for multiphoton microscopy. (thorlabs.com)
  • Multiphoton Microscopy in the Biomedical Sciences XVIII). (etis.ee)
  • In areas where the waves are out of step with each other, even by some fractional portion of a wavelength, destructive interference effects will begin to occur, attenuating or canceling the reflected light (and the color). (microscopyu.com)
  • The optical recording of the object wave formed by the resulting interference pattern of two mutually coherent component light beams. (photonics.com)
  • This letter reported the biophysical characterization of immunophenotypically distinct hematopoietic cells from normal and leukemic sources, through manipulation with optical tweezers at single cell level. (blogspot.com)
  • We demonstrate the use of holographic optical tweezers for trapping and manipulating silicon nanomembranes. (blogspot.com)
  • Finally we performed force measurements with holographic optical tweezers (HOT): The intercellular adhesion of RBCs (aggregation) exceeds a force of 25 pN. (blogspot.com)
  • The sub images were extracted from a 61-hour time-lapse microscopy video, created using quantitative phase-contrast microscopy . (wikipedia.org)
  • This enables the stain-free examination of the sperm based not only on the 2-D morphology of the cell head but also on optical thickness-based parameters, such as dry mass, and phase volume ( 5 , 6 ). (sciencemag.org)
  • In this field, it has shown unique applications for 3D characterization of technical samples and enables quantitative characterization of living cells. (wikipedia.org)
  • Contrary to conventional phase contrast images[citation needed], phase shift images of living cells are suitable to be processed by image analysis software. (wikipedia.org)
  • A population of cells with highly clustered foci inside nuclei were imaged, in 3D with submicron resolution, using an in-house developed high throughput image cytometer. (spie.org)
  • The produced quantitative phase image may have sufficient contrast for label-free auto-segmentation of cell bodies and nuclei. (patentgenius.com)
  • Fig. 2 Quantitative phase image of a drosophila melanogaster head cut obtained by digital holographic microscopy. (imperial.ac.uk)
  • The morphology and membrane fluctuation of healthy RBCs are investigated by adapting post-image processing which automatically quantify the morphological properties of RBCs, such as perimeter, mean diameter, cell volume, cell thickness and membrane fluctuation. (springerprofessional.de)
  • The phase fluctuation in the blank image without sample is 1.30 deg and the three-dimensional shape of a titanium phase test pattern is measured with an accuracy of 5.51 nm (corresponding to the phase resolution of 3.63 deg), which are better than those of off-axis systems. (spiedigitallibrary.org)
  • 6 , 7 , 8 The object image reconstructed from this complex field is free from zero-order and the twin images and can have better phase resolution than can be obtained with an off-axis system. (spiedigitallibrary.org)
  • Here we describe the use of one such method to image bacterial cells harboring the fluorescently-labelled cytokinetic FtsZ protein to show how cells are analyzed and the type of unique information that this technique can provide. (jove.com)
  • The image forming lens in traditional microscopy is thus replaced by a computer algorithm. (wikipedia.org)
  • Phase shift images Besides the ordinary bright-field image, a phase shift image is created as well. (wikipedia.org)
  • The phase shift image is unique for digital holographic microscopy and gives quantifiable information about optical distance. (wikipedia.org)
  • In reflection DHM, the phase shift image forms a topography image of the object. (wikipedia.org)
  • Instead of distorting the bright field image, transmission DHM creates a separate phase shift image showing the optical thickness of the object. (wikipedia.org)
  • The shown image of the magnetic domain structure plotted within the object aperture in Figure 1 was obtained by scanning transmission x-ray microscopy. (stanford.edu)
  • The intensities of the holographic interference pattern, which is reproduced in Figure 1 as a false color image was recorded with an in-vacuum charge-coupled device (CCD) camera. (stanford.edu)
  • It makes use of two sheared and quasi-overlapped illuminating beams experiencing relative phase shifts when going through the object, and a complementary metal-oxide-semiconductor image sensor array to record the resulting interference pattern. (sciencemag.org)
  • Some of the divergent light scattered by the specimen is captured by the objective and focused onto the intermediate image plane, where the superimposed light waves are recombined or summed through the process of interference to produce a magnified image of the specimen. (microscopyu.com)
  • Over 1000 customers worldwide use 3i's SlideBook microscopy software to manage everything from instrument control to image capture, processing and data analysis. (rms.org.uk)
  • Diseases are known to interfere with physiological function of the vision system on all three levels: They may result in failing to form a high-quality image on the retina, loss of the photoreceptor cell function, or deficiencies in the neuronal processing of the visual information. (springer.com)
  • Attenuation of the sound field induces localized acoustic streaming and the resultant convection flow gently delivers the suspended cells to the image plane where they form the designed pattern. (mpg.de)
  • An orthotopic glioblastoma mouse model maintaining brain parenchymal physical constraints and suitable for intravital two-photon microscopy. (sppin.fr)
  • The technique uses white light and can be combined with more traditional microscopy techniques, such as fluorescence, to monitor cells as they grow. (photonics.com)
  • Such optical elements may be utilized in a holographic grating, in an optical correlator system such as for pattern recognition, in a dynamic holographic recording system, and in an optical switch. (mvssolutions.com)
  • Byun H, Hillman TR, Higgins JM, Diez-Silva M, Peng ZL, Dao M et al (2012) Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient. (springerprofessional.de)
  • Provided is a small-sized quantitative phase measurement apparatus. (freepatentsonline.com)
  • The quantitative phase measurement apparatus 1 includes a reflective polarization splitting element 17 . (freepatentsonline.com)
  • Recently, a desire to understand live cell dynamics has rekindled interest in label-free phase contrast microscopy. (spie.org)
  • thus, an entire aspect of the dynamics and morphology of sperm cells is currently disregarded because of technological barriers. (sciencemag.org)
  • Multifocal ERG even provides spatial resolution, but the weak ERG signal does not allow testing of single cells. (springer.com)
  • 21 Soft X-Ray Microscopy at the ALS: 20 nm Spatial Resolution W. Chao (UCB & LBNL) W. Chao et al. (hobbydocbox.com)
  • Total-Internal-Reflection-Fluorescence (TIRF) microscopy experiments and data from a laser tweezers assay that measures in vitro microtubule assembly with nanometer resolution, provides evidence that the subunit dissociation rate from a microtubule tip increases as the free subunit concentration increases. (blogspot.com)
  • The system is optimized for long-term several-day experiments with living cells. (tescan.com)
  • In the talk, details of the novel microscopy set up and results from the resolution testing experiments will be presented. (aps.org)
  • Zeiss has granted a license for patents relating to LED fluorescence excitation in microscopy to Lumencor (Beaverton, USA). (imaging-git.com)
  • Zero-order suppression for two-photon holographic excitation. (sppin.fr)
  • Measurements do not affect the cells, enabling long term studies. (wikipedia.org)
  • As early as 1925, at a time when microscopy techniques were not developed sufficiently to study the cell membrane, Henry Fricke determined its thickness using dielectric measurements. (uwm.edu)
  • Because SLIM is completely noninvasive, the researchers can study the natural functions of cells as they occur. (photonics.com)
  • Translucent objects, like a living human cell, absorb and scatter small amounts of light. (wikipedia.org)
  • Spatial Light Interference Microscopy (SLIM) is an established method for investigating thin, transparent samples, such as single cells. (imaging-git.com)
  • 2000 nm) range light is mostly absorbed with a small scattering contribution and it only penetrates through a few cell layers. (imaging-git.com)
  • Actually, DHM has mostly been applied to light microscopy. (wikipedia.org)
  • The lateral optical resolution of digital holographic microscopy is equivalent to the resolution of traditional light microscopy. (wikipedia.org)
  • Since its invention, light microscopy has been evolving, and nowadays, a wide range of techniques is available to make "visible" what is "invisible. (sciencemag.org)
  • Most of us observe some type of optical interference almost every day, but usually do not realize the events in play behind the often-kaleidoscopic display of color produced when light waves interfere with each other. (microscopyu.com)
  • One of the best examples of interference is demonstrated by the light reflected from a film of oil floating on water. (microscopyu.com)
  • The interference effect is observed because light reflected from the inner surface of the bubble must travel farther than light reflected from the outer surface, and variations in the soap film thickness produce corresponding differences in the distances light waves must travel to reach our eyes. (microscopyu.com)
  • When the waves reflected from the inner and outer surfaces of the soap film recombine, they will interfere with each other to either remove or reinforce some wavelengths of white light by destructive or constructive interference (as illustrated in Figure 2 ). (microscopyu.com)
  • Like the soap bubble, the color is derived from interference between reflected light waves bouncing from neighboring tracks on the disk. (microscopyu.com)
  • White light reflected from the wings of the beetle produces a stunning spectral display of interference patterns similar to that originating from the surface of a compact disk. (microscopyu.com)
  • As light shines through two overlaid masks of similar phase patterns, a broad moiré pattern occurs on a screen some distance away. (wikipedia.org)
  • High-contrast innovations Carl Zeiss provides an optical polarization technique that operates with circular polarized light (Circular DIC) in contrast to conventional DIC. (zeiss.com)
  • Holographic femtosecond laser processing is very useful for high-speed processing with low-loss of light. (spie.org)
  • Does the phase of the moon affect the germination of seeds?Effect of artificial vs. natural light on plants. (science-medic.com)
  • Measuring the phase delay images of biological cells provides quantitative information about the morphology and the drymass of individual cells. (wikipedia.org)
  • When none of these physical mechanisms can produce any detectable signal, which is often the case with highly transparent objects such as biological cells and protein layers, one may still exploit phase shifts, that is, optical path differences (OPDs). (sciencemag.org)
  • Digital holographic microscopy thus makes it possible to visualize and quantify transparent objects and is therefore also referred to as quantitative phase-contrast microscopy. (wikipedia.org)
  • Clinical computed tomography (CT), as well as standard laboratory-based micro-CT set-ups, lack the contrast resolution to sufficiently visualize cartilage composition and its pathological alterations due to poor X-ray absorption of the tissue. (biomedcentral.com)
  • The first 3D images of native phase microscopic objects such as blood cells were obtained using the DHIM. (booksandbooks.com)
  • When applied to microscopy and, especially, to biological specimens, these techniques are a unique tool for obtaining well-contrasted images of label-free samples. (imperial.ac.uk)
  • To increase the contrast the specimens are stained with agents such as fluorophores [12] (chemical or genetically encoded) and dyes (including nanoparticles) [13]. (imaging-git.com)
  • Autofocus could be performed directly with fluorescence images, but due to photobleaching and destructive fluorescence by-products, it is best to minimize fluorescence exposure for photosensitive specimens and live cells. (google.com)
  • All information about the specimen is rendered visible, contrasts are sharper - and your workflow is substantially more efficient. (zeiss.com)
  • Our technical support staff has a wealth of experience in specimen preparation and microscopy techniques. (rms.org.uk)
  • Hence they are limited to counting a low number of foci per cell (5 foci per nucleus) as the quantification process is extremely labour intensive. (spie.org)
  • 2 Unbiased quantification of tissue changes Quantitative microscopy is a powerful tool in research and clinical settings, when a detailed analysis of tissue microstructure is essential to understand pathological or natural changes. (docplayer.net)
  • The interference pattern generated at the intersection of four beams from a neodymium laser was employed for the exposure of the photoresist. (ox.ac.uk)