Cell Fractionation
Subcellular Fractions
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
Chemical Fractionation
Cytoplasm
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Cell Compartmentation
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Cell Membrane
Amino Acid Sequence
Microscopy, Immunoelectron
Endoplasmic Reticulum
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
Cytosol
Golgi Apparatus
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Biological Transport
Membrane Proteins
Trichomonas
Electrophoresis, Polyacrylamide Gel
Fractionation, Field Flow
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Protein Transport
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Base Sequence
Intracellular Membranes
Cells, Cultured
Recombinant Fusion Proteins
Caveolins
Lysosomes
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Cloning, Molecular
Centrifugation, Density Gradient
Microscopy, Fluorescence
Blotting, Western
Protein Processing, Post-Translational
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
Mitochondria
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
Digitonin
HeLa Cells
Microbodies
Immunoblotting
Microsomes
Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Saccharomyces cerevisiae
Carrier Proteins
Caveolin 1
Sequence Homology, Amino Acid
Proteins
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Liver
Transfection
COS Cells
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Microscopy, Confocal
Protein Binding
Precipitin Tests
Vacuoles
Polyribosomes
A multiribosomal structure representing a linear array of RIBOSOMES held together by messenger RNA; (RNA, MESSENGER); They represent the active complexes in cellular protein synthesis and are able to incorporate amino acids into polypeptides both in vivo and in vitro. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Mutation
Fluorescent Antibody Technique, Indirect
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Brefeldin A
DNA, Complementary
3T3 Cells
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
Protein Sorting Signals
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Detergents
Peroxisomes
Nuclear Proteins
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Cricetinae
Saccharomyces cerevisiae Proteins
Nuclear Envelope
Phosphorylation
Fibroblasts
Protein Biosynthesis
Tissue Distribution
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
Endocytosis
Plasmids
Solubility
Green Fluorescent Proteins
Cytoskeleton
Cercopithecus aethiops
Chromatography, Gel
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Pancreas
A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.
Tumor Cells, Cultured
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Peptides
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Bacterial Outer Membrane Proteins
Chromatography
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
Ribosomes
Rabbits
RNA-Binding Proteins
Chromatography, Ion Exchange
Luminescent Proteins
Enzyme Activation
Cattle
Isoenzymes
Cell Line, Transformed
Chemistry Techniques, Analytical
Membrane Transport Proteins
Plant Proteins
Transcription, Genetic
Signal Transduction
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Kidney
Receptors, Cell Surface
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Phenotype
Gene Expression
Immunohistochemistry
DNA-Binding Proteins
Cytoskeletal Proteins
Gene Deletion
Sulfur Isotopes
DNA Primers
Centrifugation, Isopycnic
A technique used to separate particles according to their densities in a continuous density gradient. The sample is usually mixed with a solution of known gradient materials and subjected to centrifugation. Each particle sediments to the position at which the gradient density is equal to its own. The range of the density gradient is usually greater than that of the sample particles. It is used in purifying biological materials such as proteins, nucleic acids, organelles, and cell types.
Cell Division
Binding Sites
Actins
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
Mutagenesis, Site-Directed
Plant Extracts
Virus Replication
Hydrogen-Ion Concentration
Transcription Factors
Chromatography, DEAE-Cellulose
Protein-Serine-Threonine Kinases
Chromatography, Affinity
Amino Acids
Gene Expression Regulation
Isoelectric Focusing
Carbon Isotopes
Chromatography, High Pressure Liquid
Electrophoresis
Centrifugation
Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Fractional Precipitation
Centrifugation, Zonal
Mass Spectrometry
Substrate Specificity
Chemical Precipitation
Plants, Medicinal
Chromatography, Agarose
Carbon-Carbon Lyases
Organelles
Ultracentrifugation
Temperature
Hydroxyapatites
A group of compounds with the general formula M10(PO4)6(OH)2, where M is barium, strontium, or calcium. The compounds are the principal mineral in phosphorite deposits, biological tissue, human bones, and teeth. They are also used as an anticaking agent and polymer catalysts. (Grant & Hackh's Chemical Dictionary, 5th ed)
Chemistry
Isoelectric Point
Biological Assay
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
Carbohydrates
Chemical Phenomena
Oxygen Isotopes
Immunodiffusion
Chromatography, Thin Layer
Immune Sera
Biochemical Phenomena
Molecular Structure
Macromolecular Substances
Blood Proteins
Glycoproteins
Rats, Inbred Strains
Madagascar
One of the Indian Ocean Islands off the southeast coast of Africa. Its capital is Antananarivo. It was formerly called the Malagasy Republic. Discovered by the Portuguese in 1500, its history has been tied predominantly to the French, becoming a French protectorate in 1882, a French colony in 1896, and a territory within the French union in 1946. The Malagasy Republic was established in the French Community in 1958 but it achieved independence in 1960. Its name was changed to Madagascar in 1975. (From Webster's New Geographical Dictionary, 1988, p714)
Plant Leaves
Electrophoresis, Disc
Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.
Isotopes
Methyl Chloride
Metrizamide
Plants
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Liver Extracts
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Erythrocytes
Peptide Hydrolases
Electrophoresis, Gel, Two-Dimensional
NMD3 encodes an essential cytoplasmic protein required for stable 60S ribosomal subunits in Saccharomyces cerevisiae. (1/4968)
A mutation in NMD3 was found to be lethal in the absence of XRN1, which encodes the major cytoplasmic exoribonuclease responsible for mRNA turnover. Molecular genetic analysis of NMD3 revealed that it is an essential gene required for stable 60S ribosomal subunits. Cells bearing a temperature-sensitive allele of NMD3 had decreased levels of 60S subunits at the nonpermissive temperature which resulted in the formation of half-mer polysomes. Pulse-chase analysis of rRNA biogenesis indicated that 25S rRNA was made and processed with kinetics similar to wild-type kinetics. However, the mature RNA was rapidly degraded, with a half-life of 4 min. Nmd3p fractionated as a cytoplasmic protein and sedimented in the position of free 60S subunits in sucrose gradients. These results suggest that Nmd3p is a cytoplasmic factor required for a late cytoplasmic assembly step of the 60S subunit but is not a ribosomal protein. Putative orthologs of Nmd3p exist in Drosophila, in nematodes, and in archaebacteria but not in eubacteria. The Nmd3 protein sequence does not contain readily recognizable motifs of known function. However, these proteins all have an amino-terminal domain containing four repeats of Cx2C, reminiscent of zinc-binding proteins, implicated in nucleic acid binding or protein oligomerization. (+info)Gibberellic acid stabilises microtubules in maize suspension cells to cold and stimulates acetylation of alpha-tubulin. (2/4968)
Gibberellic acid is known to stabilise microtubules in plant organs against depolymerisation. We have now devised a simplified cell system for studying this. Pretreatment of a maize cell suspension with gibberellic acid for just 3 h stabilised protoplast microtubules against depolymerisation on ice. In other eukaryotes, acetylation of alpha-tubulin is known to correlate with microtubule stabilisation but this is not established in plants. By isolating the polymeric tubulin fraction from maize cytoskeletons and immunoblotting with the antibody 6-11B-1, we have demonstrated that gibberellic acid stimulates the acetylation of alpha-tubulin. This is the first demonstrated link between microtubule stabilisation and tubulin acetylation in higher plants. (+info)Isolation of rat liver albumin messenger RNA. (3/4968)
Rat liver albumin messenger RNA has been purified to apparent homogeneity by means of polysome immunoprecipitation and poly(U)-Sepharose affinity chromatography. Specific polysomes synthesizing albumin were separated from total liver polysomes through a double antibody technique which allowed isolation of a specific immunoprecipitate. The albumin-polysome immunoprecipitate was dissolved in detergent and the polysomal RNA was separated from protein by sucrose gradient centrifugation. Albumin mRNA was then separated from ribosomal RNA by affinity chromatography through the binding of poly(U)-Sepharose to the polyadenylate 3' terminus of the mRNA. Pure albumin mRNA migrated as an 18 S peak on 85% formamide-containing linear sucrose gradients and as a 22 S peak on 2.5% polyacrylamide gels in sodium dodecyl sulfate. It coded for the translation of authentic liver albumin when added to a heterologous protein-synthesizing cell-free system derived from either rabbit reticulocyte lysates or wheat germ extracts. Translation analysis in reticulocyte lysates indicated that albumin polysomes were purified approximately 9-fold from total liver polysomes, and that albumin mRNA was purified approximately 74-fold from albumin polysomal RNA. The total translation product in the mRNA-dependent wheat germ system, upon addition of the pure mRNA, was identified as authentic albumin by means of gel electrophoresis and tryptic peptide chromatography. (+info)Studies on a nonpolysomal ribonucleoprotein coding for myosin heavy chains from chick embryonic muscles. (4/4968)
A messenger ribonucleoprotein (mRNP) particle containing the mRNA coding for the myosin heavy chain (MHC mRNA) has been isolated from the postpolysomal fraction of homogenates of 14-day-old chick embryonic muscles. The mRNP sediments in sucrose gradient as 120 S and has a characteristic buoyant density of 1.415 g/cm3, which corresponds to an RNA:protein ratio of 1:3.8. The RNA isolated from the 120 S particle behaved like authentic MHC mRNA purified from chick embryonic muscles with respect to electrophoretic mobility and ability to program the synthesis of myosin heavy chain in a rabbit reticulocyte lysate system as judged by multi-step co-purification of the in vitro products with chick embryonic leg muscle myosin added as carrier. The RNA obtained from the 120 S particle was as effective as purified MHC mRNA in stimulating the synthesis of the complete myosin heavy chains in rabbit reticulocyte lysate under conditions where non-muscle mRNAs had no such effect. Analysis of the protein moieties of the 120 S particle by sodium dodecyl sulfate-polyacrylamide gel electrophoresis shows the presence of seven distinct polypeptides with apparent molecular weights of 44,000, 49,000, 53,000, 81,000, 83,000, and 98,000, whereas typical ribosomal proteins are absent. These results indicate that the 120 S particles are distinct cellular entities unrelated to ribosomes or initiation complexes. The presence of muscle-specific mRNAs as cytoplasmic mRNPs suggests that these particles may be involved in translational control during myogenesis in embryonic muscles. (+info)Nuclear location of mammalian DNA polymerase activities. (5/4968)
Nuclei were isolated from monolayer cultures of mouse and human cells using a nonaqueous procedure of cell fractionation in which lyophilized cells were homogenized and centrifuged in 100% glycerol. In previous work we have shown that the nuclear pellet and cytoplasmic supernatant fraction contained 10% or less of the nucleic acids characteristic of the other cell fraction. Aqueous extracts made from fresh cultures and from nonaqueous material at each step of the fractionation procedure were assayed fro DNA polymerase activity. Activities were normalized to DNA contents of extracted material. Specific activity was preserved quantitatively through freezing and drying the cells, but was found to be unstable in glycerol suspensions with approximate half-lives and 1 h at 23 degrees and 4 h at 0-4 degrees. Activities were relatively stable at -25 degrees, however, so that by homogenizing only 15 min at 4 degrees and centrifuging at -25 degrees we preserved approximately 85% of the specific activity of fresh cultures in the nonaqueous nuclear fraction. Sedimentation analyses showed that the nuclear fraction contained both DNA polymerase-alpha and-beta in approximately the proportions expected if all polymerase activities were confined to the nucleus in living cells. DNA polymerase-alpha was found to be more unstable in glycerol suspensions than DNA polymerase-beta. Nuclear location of both activities was found in exponential cultures and in 3T3 mouse cultures synchronized in the G1 and S phases of the cell division cycle. We found no evidence for cytoplasmic factors affecting nuclear polymerase activities. We have concluded that the two major DNA polymerases are nuclear although one, DNA polymerase-alpha, frequently is present as a weakly bound nuclear protein. (+info)Characterization of nuclear structures containing superhelical DNA. (6/4968)
Structures resembling nuclei but depleted of protein may be released by gently lysing cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids sediment in gradients containing intercalating agents in a manner characteristic of DNA that is intact, supercoiled and circular. The concentration of salt present during isolation of human nucleoids affects their protein content. When made in I-95 M NaCl they lack histones and most of the proteins characteristic of chromatin; in 1-0 M NaCl they contain variable amounts of histones. The effects of various treatments on nucleoid integrity were investigated. (+info)Purification of gibberellic acid-induced lysosomes from wheat aleurone cells. (7/4968)
Using isopycnic density gradient centrifugation, lysosomes were concentrated in a single region of a sucrose-Ficoll gradient (p = 1-10 g cm-3), well separated from most other cell organelles. Gibberellic acid-induced lysosomes were found to be rich in alpha-amylase and protease but not ribonuclease. The lysosomal band also contained a majority of the NADH2-cytochrome c reductase, a marker enzyme for endoplasmic reticulum, found in the gradient. Examination of electron micrographs revealed that a purified band of lyosomes contained at least 3 vesicle types, ranging in size from 0-1 to 0-5 mum. The significance of these findings to proposed mechanisms of action of gibberellic acid is discussed. (+info)Syntaxin 11 is associated with SNAP-23 on late endosomes and the trans-Golgi network. (8/4968)
SNARE proteins are known to play a role in regulating intracellular protein transport between donor and target membranes. This docking and fusion process involves the interaction of specific vesicle-SNAREs (e.g. VAMP) with specific cognate target-SNAREs (e.g. syntaxin and SNAP-23). Using human SNAP-23 as the bait in a yeast two-hybrid screen of a human B-lymphocyte cDNA library, we have identified the 287-amino-acid SNARE protein syntaxin 11. Like other syntaxin family members, syntaxin 11 binds to the SNARE proteins VAMP and SNAP-23 in vitro and also exists in a complex with SNAP-23 in transfected HeLa cells and in native human B lymphocytes. Unlike other syntaxin family members, no obvious transmembrane domain is present in syntaxin 11. Nevertheless, syntaxin 11 is predominantly membrane-associated and colocalizes with the mannose 6-phosphate receptor on late endosomes and the trans-Golgi network. These data suggest that syntaxin 11 is a SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network in mammalian cells. (+info)Best French Press Reviews of 2021 at TopProducts.com
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Cell fractionation
Cell disruption Media for cell separation by density: Percoll Ficoll Alberts, B; Johnson, A (2002). "Fractionation of Cells". ... It is the formation of homogenous mass of cells (cell homogenate or cell suspension). It involves grinding of cells in a ... In cell biology, cell fractionation is the process used to separate cellular components while preserving individual functions ... This step may not be necessary depending on the source of the cells. Animal tissue however is likely to yield connective tissue ...
Fractionation of carbon isotopes in oxygenic photosynthesis
The flux of CO2(aq) into and out of a cell is roughly proportional to the cell surface area, and the cell carbon biomass varies ... These cells are part of the characteristic kranz leaf anatomy, which spatially separates photosynthetic cell-types in a ... and variations in cell growth and geometry. The isotopic fractionations of different photosynthetic pathways are uniquely ... "Effect of Phytoplankton Cell Geometry on Carbon Isotopic Fractionation". Geochimica et Cosmochimica Acta. 62 (1): 69-77. ...
Centrifugation
"Fractionation of Cells". Molecular biology of the cell (4th ed.). New York: Garland Science. ISBN 0-8153-4072-9. Frei, Mark. " ... fractionation of subcellular organelles, fractionation of membrane vesicles, fractionation of macromolecules and macromolecular ... In biological research, cell fractionation typically includes the isolation of cellular components while retaining the ... Through low-speed centrifugation, cell debris may be removed, leaving a supernatant preserving the contents of the cell. ...
Fractionation
In cell fractionation, cell components are separated by difference in mass. A typical protocol to isolate a pure chemical agent ... The process of blood fractionation involves separation of blood into its main components. Blood fractionation refers generally ... blood cells). These separated components can be analyzed and often further separated. Fractionation is also used for culinary ... Fractionation Laboratory Handbook for Fractionation of Natural Extracts., by Peter J. Houghton and Amala Raman, publisher: ...
Jean Gruenberg
Howell, K. E.; Devaney, E.; Gruenberg, J. (1989). "Subcellular fractionation of tissue culture cells". Trends in Biochemical ... and motor-dependent fusion in vitro between apical and basolateral endocytic vesicles from MDCK cells". Cell. 62 (4): 719-731. ... His research in the fields of cell biology and biochemistry has significantly contributed to a better understanding of the ... Gruenberg, J.; Howell, K. E. (1988). "Fusion in the endocytic pathway reconstituted in a cell-free system using immuno-isolated ...
Chromatolysis
Other stages included cell fractionation into smaller bodies. Flemming named this degenerative process "chromatolysis" to ... It is an induced response of the cell usually triggered by axotomy, ischemia, toxicity to the cell, cell exhaustion, virus ... The cells exhibited decreased numbers of Nissl bodies throughout the cell, especially at the peripheral cytoplasm were the ... The cytoskeleton maintains the nuclear components of a cell and the size of the cell body in neurons. The increase in protein ...
Sialidase-2
Its cytosolic localization was demonstrated by cell fractionation experiments. GRCh38: Ensembl release 89: ENSG00000115488 - ... Expression studies in COS-7 cells confirmed that this gene encodes a functional sialidase. ... Cell Res. 17 (4): 357-362. doi:10.1038/cr.2007.27. PMID 17426694. Chavas LM, Tringali C, Fusi P, et al. (2005). "Crystal ...
Kinetic isotope effects of RuBisCO
"Effect of Phytoplankton Cell Geometry on Carbon Isotopic Fractionation". Geochimica et Cosmochimica Acta. 62 (1): 69-77. ... Organisms using RuBisCOs with high values of VC / KC, and low values of Sc/o have localized RuBisCO to areas within the cell ... Isotope geochemistry Fractionation of carbon isotopes in oxygenic photosynthesis Isotopes of carbon Isotopic signature Farquhar ... Farquhar, G. D.; Hubick, K. T.; Condon, A. G.; Richards, R. A. (1989), "Carbon Isotope Fractionation and Plant Water-Use ...
Homogenizer
Cell fractionation is done by homogenizer to release the organelles from cell. Whereas older technologies just focused on the ... Homogenization (chemistry) French pressure cell press Cell disruption Ultrasonic homogenizer "Homogenizers for Mixing, ... There are often many different names for the same piece of mechanical homogenizing equipment, including Cell Lysor, Disperser, ... Homogenization is a very common sample preparation step prior to the analysis of nucleic acids, proteins, cells, metabolism, ...
Cell survival curve
Dose fractionation Dose-response relationship Chronic radiation syndrome "Cell Survival Curves" (PDF). Massachusetts Institute ... A cell survival curve is a curve used in radiobiology. It depicts the relationship between the fraction of cells retaining ... Cell survival fractions are exponential functions with a dose-dependent term in the exponent due to the Poisson statistics ... The linear quadratic model is now most often used to describe the cell survival curve, assuming that there are two mechanisms ...
Outline of cell biology
... especially animal cells. Cell disruption, and cell unroofing - Methods for releasing molecules from cells. Cell fractionation ... Meristemic cell - Undifferentiated plants cells analogous to animal stem cells. Stem cell - Undifferentiated cells found in ... Cell type - Distinct morphological or functional form of cell. When a cell switches state from one cell type to another, it ... Cell biology - (formerly cytology) The study of cells. Cell division - The process of one parent cell separating into two or ...
Erythrocytapheresis
In erythrocytapheresis, centrifugation is the most commonly used red blood cell fractionation method. This is because the ... The centrifugal force will separate the red blood cells from other cells due to their high specific weight. These cells can ... This includes either two standard units of red blood cells or one unit plus of red blood cells and another of either plasma or ... After removal of the affected cells, the patient can then be transfused with a dose of normal red blood cells. ...
CGAS-STING cytosolic DNA sensing pathway
Through biochemical fractionation of cell extracts and quantitative mass spectrometry, Sun, et al. identified cGAS as the DNA- ... and T cells. In many tumor cells, the DNA damage response is constitutively active, leading to the accumulation of cytoplasmic ... Cell. 176 (6): 1432-1446.e11. doi:10.1016/j.cell.2019.01.049. PMC 6697112. PMID 30827685. Volkman HE, Cambier S, Gray EE, ... "Cell intrinsic immunity spreads to bystander cells via the intercellular transfer of cGAMP". Nature. 503 (7477): 530-4. Bibcode ...
Organelle
... and can also be purified by cell fractionation. There are many types of organelles, particularly in eukaryotic cells. They ... While most cell biologists consider the term organelle to be synonymous with cell compartment, a space often bound by one or ... In cell biology, an organelle is a specialized subunit, usually within a cell, that has a specific function. The name organelle ... Not all eukaryotic cells have each of the organelles listed below. Exceptional organisms have cells that do not include some ...
Nucleoid
Cell-fractionation and electron microscopy studies first indicated the possibility of DNA-membrane connections. There are now ... 1/cell volume in liter) x 103. Cell volume in liter ( 2 x 10−15) was determined by assuming volume of the E. coli cell to be 2 ... coli cells: analogous measurements in insect and human cells". Cell. 21 (3): 773-83. doi:10.1016/0092-8674(80)90440-7. PMID ... coli nucleoid organization and dynamics in living cells". Cell. 153 (4): 882-95. doi:10.1016/j.cell.2013.04.006. PMC 3670778. ...
Christian de Duve
His work on cell fractionation provided an insight into the function of cell structures. He specialized in subcellular ... of the cell fractionation procedure) had come from membranous fractions that were known to be cell organelles. In 1955, de Duve ... So they tried the more laborious procedure of cell fractionation to detect the enzyme activity. This was the moment of ... Later, prokaryotic cells were incorporated to form more organelles. De Duve proposed that peroxisomes, which allowed cells to ...
Cell biology
... and cell composition. The study of cells is performed using several microscopy techniques, cell culture, and cell fractionation ... Direct cell-cell contact is when a receptor on a cell binds a molecule that is attached to the membrane of another cell. ... Cell signaling or cell communication is important for cell regulation and for cells to process information from the environment ... Biology portal Science portal The American Society for Cell Biology Cell biophysics Cell disruption Cell physiology Cellular ...
Alex B. Novikoff
After he developed cell fractionation method, it became possible to identify and isolate cell organelles. He was the first to ... Novikoff had successfully developed a technique of cell fractionation. Using this he had separated cell fractions and ... Novikoff, PM; Novikoff, AB (1972). "Peroxisomes in absorptive cells of mammalian small intestine". The Journal of Cell Biology ... His works in cell biology are best summed up in a textbook he wrote with his student Eric Holtzman, Cells and Organelles, first ...
Albert Claude
In 1930 he developed the technique of cell fractionation, by which he discovered the agent of the Rous sarcoma, components of ... The process consists of grinding up cells to break the membrane and release the cell's contents. He then filtered out the cell ... In 1930, he discovered the process of cell fractionation, which was groundbreaking in his time. ... He discovered that mitochondria are the "power houses" of all cells. He also discovered cytoplasmic granules full of RNA and ...
Great Lakes
Effect of Phytoplankton Cell Geometry on Carbon Isotopic Fractionation, Vol. 62, Iss. pp. 69-77. Durbin, E.G. (1977), "Studies ... The Influence of Cell Size on Growth Rate, and Carbon, Nitrogen, Chlorophyll a and Silica Content". Journal of Phycology, 13: ... Although a drop of lake water may contain 1 million bacteria cells and 10 million viruses, only since 2012 has there been a ... There is a positive correlation between the surface area and the chlorophyll concentration of diatom cells. Several Native ...
Visual artifact
Different techniques including freeze-fracturing and cell fractionation may be used to overcome the problems of artifacts. A ... Stacking of cells on top of each other gives a dark look, and in this breast tissue it may mimic microcalcifications. Pap ... Inflammatory and tumor cells are most susceptible to crush artifacts. Cellulose contamination, in H&E stain and polarized light ... Staining can cause the appearance of solid chemical deposits that may be seen as structures inside the cell. ...
Field flow fractionation
Split flow thin-cell fractionation (SPLITT) is a special preparative FFF technique, using gravity for separation of µm-sized ... Field-flow fractionation, abbreviated FFF, is a separation technique which does not have a stationary phase. It is similar to ... Separation in Field flow fractionation takes place in a channel. It is composed of a top and bottom block which are separated ... As mentioned above, in field-flow fractionation the field can be hydraulic (with a cross flow through a semi-permeable membrane ...
Isotope electrochemistry
Bioelectrochemical reactor Concentration cell Electrochemical cell Electrochemical engineering Equilibrium fractionation ... electrochemical isotope separation electrochemical isotope cell ACS radioelectrochemistry electroplating isotope effects JES ... Transient kinetic isotope fractionation Rock, Peter A. (1975). "The Electrochemical Determination of Equilibrium Constants for ... electrochemical pumping isotope effects electrochemical isotope fractionation https://web.archive.org/web/20120319145651/http ...
Dose fractionation
... which produce blood cells), and the loss of other cells in the wall of the intestines, that is fatal. Fractionation effects are ... so caution should be used in using results for a cancer cell line to make predictions for healthy cells if the cancer cell line ... The human body contains many types of cells, and the human can be killed by the loss of a single type of cell in a vital organ ... surviving fraction have been drawn for a hypothetical group of cells with and without a rest time for the cells to recover. ...
Brown algae
"Chemical and enzymatic fractionation of cell walls from Fucales: Insights into the structure of the extracellular matrix of ... As this apical cell divides, the new cells that it produces develop into all the tissues of the alga. Branchings and other ... There are no known species that exist as single cells or as colonies of cells, and the brown algae are the only major group of ... The cell wall consists of two layers; the inner layer bears the strength, and consists of cellulose; the outer wall layer is ...
Reinhard Dallinger
Subcellular fractionation of metal-accumulating lysosomes from hepatopancreas. In: Cell Biol Toxicol. 4:97-109 Dallinger R., ... In: Mol Cell Biochem. 85: 135-145 Gruber C., Stürzenbaum S., Gehrig P., Sack R., Hunziker P., Berger B., Dallinger R. (2000) ( ... In: Cell Biol. Toxicol. 4:81-96 Dallinger R., Prosi F., 1988: Heavy metals in the terrestrial isopod Porcellio scaber Latreille ... In: Cell Mol Biology 46: 331-346 Egg M., Höckner M., Chabicovsky M., Brandstätter A., Schuler D., Dallinger R. (2009) ...
Zanvil A. Cohn
His interest in Rockefeller stemmed from the fact that many of the advances, in electron microscopy, cell fractionation, and ... this cell, which Steinman named the dendritic cell, would later be found "to be the sentinel cells of the immune system." In ... J. Cell Biol. 86:304-314. With C. F. Nathan and H. W. Murray. The macrophage as an effector cell. N. Engl. J. Med. 303:622-626 ... The enzymatic iodination of the red cell membrane. J. Cell Biol. 55:390-405. With R. M. Steinman. The interaction of soluble ...
Free-flow electrophoresis
At the end of the separation cell, the separated sample is split up at the fractionation tubes and collected in microtiter ... cells, subcellular compartments (like organelles, ribosomes, etc.), Separation size range from ions to complete cells Sample ... Until the 1980s, it was a standardized technology for the separation of cells and organelles, and FFE was even tested in space ... The front plate also contains the inlets for the separation buffers and the sample, the outlets for the fractionation tubes and ...
Deductive-nomological model
... cell fractionation and advancements in staining and fixation). James Fetzer, ch 3 "The paradoxes of Hempelian explanation", in ... the molecular biology research program cracked a genetic code in the early 1960s and then converged with cell biology as cell ... Discovering Cell Mechanisms: The Creation of Modern Cell Biology (New York: Cambridge University Press, 2006). Bechtel, William ... cell biology arose and established existence of cell organelles besides the nucleus. Launched in the late 1930s, ...
George Emil Palade
The prize was granted for his innovations in electron microscopy and cell fractionation which together laid the foundations of ... The first relied exclusively on cell fractionation, and was developed in collaboration with Philip Siekevitz, Lewis Joel Greene ... Tartakoff, Alan M (November 2002). "George Emil Palade: charismatic virtuoso of cell biology". Nature Reviews Molecular Cell ... was a Romanian cell biologist. Described as "the most influential cell biologist ever", in 1974 he was awarded the Nobel Prize ...
Ammonia
... can also be used as a source of hydrogen for acid fuel cells if the unreacted ammonia can be removed. Ruthenium and ... Millar, T. J. (2003). "Deuterium Fractionation in Interstellar Clouds". Space Science Reviews. 106 (1): 73-86. Bibcode:2003SSRv ...
Mary Locke Petermann
In earlier work using cell fractionation to investigate the content of animal cells, Albert Claude found a pool of particles ...
Linear no-threshold model
For example, a hot particle embedded in an organ (such as lung) results in a very high dose in the cells directly adjacent to ... DNA repair Dose fractionation Nuclear power debate#Health effects on population near nuclear power plants and workers Radiology ... January 2012). "Evidence for formation of DNA repair centers and dose-response nonlinearity in human cells". Proceedings of the ... such as DNA repair and programmed cell death, that would protect it against carcinogenesis due to low-dose exposures of ...
Bioarchaeology
The fractionation of 34S (δ34S) is particularly useful since it is the most abundant of the rare sulfur isotopes, allowing the ... That as the body attempts to compensate for low iron levels by increasing red blood cell production in the young, sieve-like ... These fractionations are then reported relative to Canyon Diablo Troilite (V-CDT), the agreed upon standard for the field. The ... The science is based on the preferential fractionation of lighter or heavier isotopes, which results in enriched and depleted ...
Mauro Ferrari
2013). "Synthetic nanoparticles functionalized with biomimetic leukocyte membranes possess cell-like functions". Nature ... "Combinational multidomain mesoporous chips and a method for fractionation, stabilization, and storage of biomolecules", ... "Particles for cell targeting", published 2013 US patent 8568877, Ferrari M, Liu X, Chiappini C, Fakhoury JR, "Porous and non- ... "Particle compositions with a pre-selected cell internalization mode", published 2012 US patent 7993271, Liu J, Ferrari M, ...
Alan Hall
DNA from a rhabdomyosarcoma cell line and a fibrosarcoma cell line transformed a NIH/3T3 mouse fibroblast cell line. After ... After size fractionation of FCS and analysis of the lipids that bound to serum albumin, the lysophosphatidic acid (LPA) was ... "Prominent Cell Biologist Dies". In Memoriam: Alan Hall, Pioneer in Rho GTPases and Chair of Cell Biology at Sloan-Kettering "At ... cells. Downregulation of RhoA in the HBE cell lines using siRNAs showed a lack of apical junction formation in contrast with ...
Archean life in the Barberton Greenstone Belt
see Isotope analysis section) Cells are preserved in the rock record because their cell walls are made of proteins which ... Stable isotope fractionation is a useful way of characterising organic carbon and inorganic carbon. These numbers are reported ... These cell-sized prokaryote fossils are seen in the Barberton fossil record in rocks as old as 3.5 billion years. The Barberton ... The cell wall structure can be preserved, but the original composition changes over time and becomes mineralised. There are six ...
Dose-response relationship
... cell or population response). A commonly used dose-response curve is the EC50 curve, the half maximal effective concentration, ... Certain safety factor Hormesis Pharmacodynamics Spatial epidemiology Weber-Fechner law Dose fractionation Crump, K. S.; Hoel, D ...
Spatial memory
Place cells are also found in the hippocampus. The parietal cortex encodes spatial information using an egocentric frame of ... Within the literature, it is suggested that further research into the fractionation of STM and WM be explored. However, much of ... Brun, V. H.; Otnaess, M. K.; Molden, S.; Steffenach, H.; Witter, M. P.; Moser, M.; Moser, E. I. (2002). "Place cells and place ... Participants are presented with a series of matrix patterns that have half their cells colored and the other half blank. The ...
Pea protein
... can be produced using two methods: The wet fractionation method; and The dry fractionation method. The wet ... also aids digestibility as it is attributed to the nonavailability to amylases of starch granules enclosed in intact cell wall ... Dry fractionation is a more sustainable method of processing as it does not require the use of water and energy is not required ... The dry fractionation method is used to produce pea protein concentrates. It involves dry milling technology; a traditional ...
RuBisCO
Xi J, Wang X, Li S, Zhou X, Yue L, Fan J, Hao D (November 2006). "Polyethylene glycol fractionation improved detection of low- ... There are typically several related small-chain genes in the nucleus of plant cells, and the small chains are imported to the ... Lodish H, Berk A, Zipursky SL, Matsudaira P, Baltimore D, Darnell JE (2000). "Molecular Cell Biology" (4th ed.). New York: W. H ... Cooper GM (2000). "10.The Chloroplast Genome". The Cell: A Molecular Approach (2nd ed.). Washington, D.C: ASM Press. ISBN 978-0 ...
Oxygen-18
Water molecules are also subject to Rayleigh fractionation as atmospheric water moves from the equator poleward which results ... An example of the production cycle is a 90-minute irradiation of 2 milliliters of 18O-enriched water in a titanium cell, ... the temperature of ice formation can be calculated as equilibrium fractionation between phases of water that is known for ... and ions leached from the target cell and sputtered from the Havar foil. Willi Dansgaard - a paleoclimatologist Isotopes of ...
Radiation therapy
Fractionation also allows tumor cells that were in a relatively radio-resistant phase of the cell cycle during one treatment to ... Fractionation allows normal cells time to recover, while tumor cells are generally less efficient in repair between fractions. ... Targeting double-stranded breaks increases the probability that cells will undergo cell death. Cancer cells are generally less ... Single-strand DNA damage is then passed on through cell division; damage to the cancer cells' DNA accumulates, causing them to ...
Carbon-13
Isotopes of carbon Isotope fractionation "Exact Masses of the Elements and Isotopic Abundances". sisweb.com. Francey, R. J.; ... One important application is in stable isotope labeling by amino acids in cell culture (SILAC). 13C-enriched compounds are used ... Biological processes preferentially take up the lower mass isotope through kinetic fractionation. In aqueous geochemistry, by ...
Lithium
1990). Lithium and Cell Physiology. New York, NY: Springer New York. pp. 25-46. doi:10.1007/978-1-4612-3324-4. ISBN 978-1-4612- ... Nuclear weapons manufacture and other nuclear physics applications are a major source of artificial lithium fractionation, with ... A typical lithium-ion battery can generate approximately 3 volts per cell, compared with 2.1 volts for lead-acid and 1.5 volts ... "You've got the power: the evolution of batteries and the future of fuel cells" (PDF). Toshiba. Archived (PDF) from the original ...
Stromatoxin
Its target channels can be found in cardiac tissue, neurons and smooth muscle cells. In cardiac cells, their role more ... 2002). Bioassay guided fractionation and chromatography identified stromatoxin as the functional component. The full sequence ... Cell Physiol., 291 (2): 348-356, doi:10.1152/ajpcell.00086.2006, PMID 16571867. Shiau, Y.S.; Huang, P.T.; Liou, H.H.; Liaw, Y.C ... Wang, D.; Schreurs, B.G. (2006), "Characteristics of IA currents in adult rabbit cerebellar Purkinje cells", Brain Res., 1096 ( ...
Phycotoxin
Excreted toxins would seem most advantageous to the individual cell in their ability to keep predators and/or parasitic and ... Most commonly, the presence of a phycotoxin is verified by bioassay-guided fractionation. The sample must be fractionated, or ... Post-ingestion toxins, also known as suicide toxins, are not beneficial to individual cells because unlike terrestrial plants, ... These toxins are located at the cell surface and are typically classified as glycoproteins, glycolipids, or polypeptides. These ...
Eurofins Scientific
The group focuses on "G protein coupled receptors," a major class of proteins in human cells, on which many molecules can act. ... Assay Site-Specific Natural Isotope Fractionation-Nuclear Magnetic Resonance (SNIF-NMR) - used by Eurofins for the analysis of ...
Ventricular fibrillation
Myocardial cells are exposed to different environments. Normal cells may be exposed to hyperkalaemia; abnormal cells may be ... "Primary ventricular fibrillation is associated with increased paced right ventricular electrogram fractionation". Circulation. ... When myocardial cell are exposed to hyperkalemia, the maximum diastolic potential is depolarized as a result of the alteration ... For example, with a healed myocardial infarction, abnormal cells can be exposed to an abnormal environment such as with a ...
CSL Limited
In August 2017, the business announced it would acquire Calimmune and its stem cell therapy platform. Completed in 2018, ... In 1952, operation commenced plasma fractionation. Thereafter the range of antivenoms increased, including those against other ... CSL's product areas include blood plasma derivatives, vaccines, antivenom, and cell culture reagents used in various medical ... Sequirus Commonwealth Serum Laboratories "CSL Behring Acquires Biotech Company Calimmune and its Proprietary Stem Cell Gene ...
University of Reims Champagne-Ardenne
IFR 53 Epidemiological surveillance of vector-borne and parasitic diseases JE 2533 Extracellular matrix and cell dynamics ( ... CNRS UMR 6089 Fractionation of Agricultural Resources and Environment (FARE) UMR INRA 614-A Research Unit of the Vine and Wine ... INSERM UMR-S 926 Cellular and molecular mechanisms involved in the pre-and postconditioning myocardial EA 3801 Cell ... Research Institute Federations interactions Cells Micro-Environment. ...
Young-Ki Paik
Potential Regulatory Role of Human-Carboxylesterase-1 Glycosylation in Liver Cancer Cell Growth. Y., K. Paik; J., E. Graham; M ... "Biomarker discovery from the plasma proteome using multidimensional fractionation proteomics". Current Opinion in Chemical ... Aging Cell. 8 (4): 473-483. doi:10.1111/j.1474-9726.2009.00495.x. ISSN 1474-9726. PMID 19489741. S2CID 593237. Na, Keun; Jeong ... CA and started his postdoc carrier with John Taylor on molecular cell biology of human apolipoprotein E gene which is involved ...
Sulfate-reducing microorganism
Many organisms reduce small amounts of sulfates in order to synthesize sulfur-containing cell components; this is known as ... Sulfur mass-independent fractionation in subsurface fracture waters indicates a long-standing sulfur cycle in Precambrian rocks ...
Serum albumin
Bovine serum albumin, or BSA, is commonly used in immunodiagnostic procedures, clinical chemistry reagents, cell culture media ... Blood plasma fractionation Bovine serum albumin Chromatography in blood processing Human serum albumin Lactalbumin Ovalbumin ...
"Altered Fractionation of Radical Radiation Therapy in the Management of Unresectable Non-Small Cell Lung Cancer" by E....
cell-fractionation-v19 - Practically Science
Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors. - Oxford Stem Cell Institute
1. power c) Cell fractionation d) Scanning electron Research paper for students
The cell theory states that a) New cells come from preexisting cells b) All cells are descended from ancient cells ) Cells ... 1. power c) Cell fractionation d) Scanning electron. 1.. The golgi complex functions to a) Modify proteins b) Process proteins ... Cell fractionation d) Scanning electron microscopy e) Phase contrast 3. Detailed information about the shape and external ... Label the diagrams of the animal and plant cells? How is the structure of each organelle related to its function? ...
Blood Fractionation Protocol for Collection of White Blood Cells | Thermo Fisher Scientific - US
SICOF | Karolinska Institutet
Our goal is to facilitate the use of single cell/nuclei sequencing technologies for all researchers. ... The Single Cell Core Facility for Flemingsberg campus (SICOF) is a state of the art core facility, offering services to the ... fractionations ... Microbiology, Tumor and Cell Biology * Molecular Medicine and ...
Archaeal TFEα/β is a hybrid of TFIIE and the RNA polymerase III subcomplex hRPC62/39 | eLife
Cell lysate fractionation. Request a detailed protocol S. solfataricus P2 cells were grown in Brock medium, 0.1% NZ-amine, 0.2 ... cells from plasmid p988. Cells were resuspended in N(300) and disrupted using a using a French Pressure cell at 16,000 psi. The ... Cells were disrupted by threefold passage through a French pressure cell (Thermo Scientific) at 16,000 psi. Cell debris was ... All samples were immediately chilled on ice, cells were harvested by centrifugation and stored at −80°C. Cells were then ...
Separation of cancer cells from white blood cells by pinched flow fractionation. - MRC Weatherall Institute of Molecular...
... is used to separate cancer cells from white blood cells (WBCs). The cells are separated at efficiencies above 90% for both cell ... Circulating tumor cells (CTCs) are found in the blood of cancer patients and can form new tumors. CTCs are rare cells in blood ... The separation is challenged by the size overlap between cancer cells and the 10(6) times more abundant WBCs. The size overlap ... which also enables further analysis of the separated cells. ... however we demonstrate that cell deformability can be exploited ...
Comparison of altered fractionation schedule with concurrent chemo-radiation for squamous cell carcinoma of head and neck | K. ...
Comparison of altered fractionation schedule with concurrent chemo-radiation for squamous cell carcinoma of head and neck ... Comparison of altered fractionation schedule with concurrent chemo-radiation for squamous cell carcinoma of head and neck. ... Acute toxicity, Altered fractionation, Concurrent chemo-radiation, Radiotherapy, Squamous cell carcinoma of head and neck ... Accelerated fractionation compared to conventional fractionation improves loco regional control in radiotherapy of advanced ...
WikiGenes
Nonaqueous fractionation of HeLa cells in glycols.. A further modification of Behrens method of nonaqueous cell fractionation ... Nonaqueous fractionation of HeLa cells in glycols. Gurney, T., Collard, M.W. Anal. Biochem. (1984) [Pubmed] ... The chief reason for use of the glycol method instead of other methods of cell fractionation is that easily solubilized ... HeLa cells were frozen in melting Freon-12 ( CCl2F2 ), dried under vacuum at -30 degrees C, sonicated in hexylene glycol at -35 ...
Antiandrogen-induced cell death in LNCaP human prostate cancer cells | Cell Death & Differentiation
... and cell adhesion. In contrast, cells treated with Casodex display loss of cell adhesion, but sustained mitochondrial ... Overexpression of Bcl-2 in LNCaP cells attenuates the induction of cell death by TNF-α but not Casodex, suggesting that ... of the cells by 48 h in a dose-dependent manner. In cells treated with TNF-α, this is accompanied by the loss of mitochondrial ... non-metastatic LNCaP human prostate cancer cells with 0-100 μ M Casodex or 0-10 ng/ml TNF-α induces cell death in 20-60% ...
A Comprehensive Immunization Strategy to Eliminate Transmission of Hepatitis B Virus Infection in the United States:...
Inactivation and partition of human T-cell lymphotrophic virus, type III, during ethanol fractionation of plasma. Transfusion ... Bauer T, Jilg W. Hepatitis B surface antigen-specific T and B cell memory in individuals who had lost protective antibodies ... antigen-specific B and T cell proliferation (160). Although direct measurement of immune memory is not yet possible, these data ... hematopoietic stem-cell transplant recipients, and patients undergoing chemotherapy) (145--147). Modified dosing regimens, ...
Hemoglobin Electrophoresis: MedlinePlus Medical Test
Its used to diagnose anemia, sickle cell disease, and other hemoglobin disorders. Learn more. ... Hemoglobin is a protein in your red blood cells that carries oxygen. Hemoglobin electrophoresis is a test that measures ... Other names: Hb electrophoresis, hemoglobin evaluation, hemoglobinopathy evaluation, hemoglobin fractionation, Hb ELP, sickle ... Sickle cell trait. In this condition, you have one sickle cell gene and one normal gene. Most people with sickle cell trait do ...
Radiation Necrosis: Background, Pathophysiology, Epidemiology
Tumor cells have less ability than healthy cells for DNA repair. Thus, between fractionation doses, healthy cells have a ... With each subsequent mitosis, the cumulative effects of unrepaired DNA result in apoptosis (cell death) of these tumor cells. ... Genetic Profiling Identifies High-Risk Tumors in Cutaneous Squamous Cell Carcinoma * Mohs Surgery Saves Costs and May Improve ... The ratio of HLA-DR and VNN2+ expression on CD14+ myeloid derived suppressor cells can distinguish glioblastoma from radiation ...
Search results - ORA - Oxford University Research Archive
Neutralizing-antibody-mediated protection of chickens against infectious bursal disease via one-time vaccination with...
Nisin-controlled expression, cell fractionation and protein extraction. Nisin-controlled expression was carried out as ... c Western immunoblot analysis localization of VP2-RCK protein in recombinant r-L. lactis-OptiVP2-RCK cells. The cell surface ... Cell wall fractions were prepared for further analysis as previously described [44, 45], Briefly, For cell wall preparations, ... cell lines, with the highest invasion level obtained in MA104 cells. However, just as importantly, only a 46 amino acid region ...
Structural and functional features and significance of the physical linkage between ER and mitochondria | Journal of Cell...
The role of mitochondria in cell metabolism and survival is controlled by calcium signals that are commonly transmitted at the ... Fractionation of RBL-2H3 cells Cells grown overconfluent in tissue culture flasks (∼7 × 107 cells) were loaded with fura2FF/AM ... Cells RBL-2H3 cells were cultured as described previously (Csordas et al., 1999). Cells were transfected with cDNA by means of ... 4 A, bottom). In control cells, the first [Ca2+]c rise was similar to those in the OMM-ER linker cells; however, the second [Ca ...
Search | Canadian Forest Service Publications | Natural Resources Canada
Update on Immunization - Recommendations of the ACIP
Inactivation and partition of human T-cell lymphotrophic virus, type III, during ethanol fractionation of plasma. Transfusion ... Evaluation of a human diploid cell strain rabies vaccine: final report of a three year study of pre-exposure immunization. J ... Laboratory studies have shown that the margin of safety based on the removal of HIV infectivity by the fractionation process is ... Rubella vaccine is prepared in human diploid cell cultures and has rarely been reported to be associated with allergic ...
Radiotherapy Gives Long-Term Disease Control in Prostate Cancer
"The so-called fractionation of radiation is thought to help preferentially kill tumor cells and minimize chronic tissue damage ... The higher-dose fractionation group was also associated with a lower rate of distant metastases, at 5%, compared with 11% for ... "This fractionation schedule appears to be acceptable for patients across all risk groups," Abu-Gheida observed. ... Grades 2 and 3 late genitourinary (GU) toxicity was higher with the higher fractionation dose, at 14.6%, compared with 8.2% for ...
Frontiers | SINEUPs are modular antisense long non-coding RNAs that increase synthesis of target proteins in cells
SINEUPs function in an array of cell lines and can be efficiently directed toward N-terminally tagged proteins. Their ... SINEUPs function in an array of cell lines and can be efficiently directed towards N-terminally tagged proteins. Their ... synthetic SINEUPs targeting endogenous Parkinsons disease-associated DJ-1 and proved to be active in different neuronal cell ... Cellular Fractionation. For fractionation experiments, GFP/SINEUP-GFP were transfected in 10 cm plates at 1:6 ratio using ...
MojoSort Mouse CX3CR1 Selection Kit
Cells were gated on CD11b and CX3CR1. The bottom plot shows cells after percoll fractionation. Debris and Dead cells were gated ... Cells were gated on CD11b and CX3CR1. The bottom plot shows cells after percoll fractionation. Debris and Dead cells were gated ... Cell Separation (MojoSort™) - Quality tested. Recommended Usage 10 µl of antibody cocktail for 1 X 106 cells in 100 µl of ... Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers Molecular Family Cytokine/Chemokine Receptors, GPCR Gene ID ...
Structural characterisation of TNRC6A nuclear localisation signal in complex with importin-alpha | PLOS ONE
... immunostaining and cell fractionation analyses have demonstrated strong nuclear localisation of TNRC6A in some cancer cell ... Gagnon Keith T, Li L, Chu Y, Janowski Bethany A, Corey David R. RNAi Factors Are Present and Active in Human Cell Nuclei. Cell ... Human Impα1ΔIBB (hImpα1ΔIBB) was expressed in BL21(DE3) (NEB) cells in LB media at 25°C for 5 hours after induction with IPTG, ... Specific and potent RNAi in the nucleus of human cells. Nat Struct Mol Biol. 2005;12(2):133-7. pmid:15643423 * View Article ...
A regulatory domain in the C-terminal extension of the yeast glycerol channel Fps1p<...
We have performed growth tests, membrane localization following cell fractionation, and glycerol accumulation measurements as ... We have performed growth tests, membrane localization following cell fractionation, and glycerol accumulation measurements as ... We have performed growth tests, membrane localization following cell fractionation, and glycerol accumulation measurements as ... We have performed growth tests, membrane localization following cell fractionation, and glycerol accumulation measurements as ...
Hypothesis
Cell fractionation kit was obtained from Fermentas (USA). Kitfor TUNEL assay kit wasobtained from Invitrogen(Carlsbad, CA, USA ... Media for cell culture (DMEM and Hams F12) and foetal bovine serum (FBS) were obtained from Gibco, Invitrogen (Carlsbad, CA, ... This property enables pACYC184 to co-exist in cells with ColE1 plasmid vectors, including all the ones mentioned above ... pACYC184 is a medium-copy-number cloning vector (~ 20 copies/cell) with Cmr and Tetrselectable markers. It carries the origin ...
Expression of AE1/p16 promoted degradation of AE2 in gastric cancer cells | BMC Cancer | Full Text
AE2 expression and pHi were measured after knockdown or forced expression of AE1 or p16 in GC cells. The effect of AE2 on GC ... AE1/p16 expression promoted AE2 degradation in GC cells. Gastrin is a potential candidate drug for targeted therapies for AE1- ... The relationship among AE1, AE2 and p16 in GC cells was characterized by molecular and cellular experiments. ... Gastrin inhibited the growth of GC cells at least partially through up-regulation of AE2 expression. ...
Proteomes | Free Full-Text | Differential Proteome Analysis of Extracellular Vesicles from Breast Cancer Cell Lines by...
... released from cancer cells in an accelerated manner compared to normal cells. The Vn96 peptide was herein employed to recover a ... Reflecting their cells of origin, cancer EVs displayed discrete differences from those of normal phenotype. GELFrEE LC/MS ... The protein data sets provide valuable information from material shed by cultured cells. It is probable that a vast amount of ... Pathway analysis of the Vn96-affinity proteome unequivocally distinguished EVs from tumorigenic cell lines (SKBR3 and MCF-7) ...
Whole Cell Lysis Reagents & Enhancers
... cell lysis buffers, and reagents for solubilizing proteins from bacteria, yeast, and insect cultures, as well as plant and ... Subcellular Fractionation, Enrichment, and Depletion Reagents. Subcellular fractionation and protein enrichment enable the ... A variety of reagents and enzymes for cell lysis and protein extraction provide an array of options tailored for specific cell ... CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. ...
Automated Blood Fractionation
Discover our Blood fractionation workstations for the automated processing of whole blood into separate components of plasma, ... For the automated processing of whole blood into separate components of plasma, buffy layer or red blood cells for downstream ... High-Throughput, End-to-End Cell Free DNA Analysis Workflow from Plasma ... buffy coat and red blood cells. The blood components can be reformatted into nearly any consumable for downstream storage or ...
Standard fractionation radiotherapyProteinsTumor cellsSubcellularEfficient cell lysisEukaryotic cellsRadiotherapyChromatin2019LocalizationMammalian cellsLysisCellularEpithelialConventionalCytoplasmicConcurrentNucleusHeLaGeneticNucleiMitochondriaChromatographyPlasmaWhite BloodElectronInhibitionSoybeanReagentsDigestion2016AssaysToxicityDegradationCompartmentFlow cytometryAcuteEndothelial cellsSurvivalVitroTumorsMembraneQualifierCancer cellsEmbryonicSerumDoseTransformationTechniquesMarkersBufferSeparationDownstreamCulturesBiolYeast cellOxidative stressPathway
Standard fractionation radiotherapy1
- A Radiation Therapy Oncology Group (RTOG) phase III randomized study to compare hyper-fractionation and two variants of accelerated fractionation to standard fractionation radiotherapy for head and neck squamous cell carcinomas: First report of RTOG 9003. (msjonline.org)
Proteins11
- SINEUPs function in an array of cell lines and can be efficiently directed toward N-terminally tagged proteins. (frontiersin.org)
- Isolation and purification of proteins requires disruption of cells or tissue samples and extraction of the relevant protein fraction. (sigmaaldrich.com)
- Radioimmunoprecipitation assay (RIPA) buffer is used for rapid cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. (sigmaaldrich.com)
- CelLytic™ lysis reagents are specifically formulated to lyse and extract cellular proteins under non-denaturing conditions based on the cell or tissue type and target protein localization. (sigmaaldrich.com)
- Subcellular fractionation and protein enrichment enable the identification and study of proteins in proteomics research. (sigmaaldrich.com)
- Transactivator (TAT) proteins are known to have cell-penetrating properties. (biomedcentral.com)
- The transactivator (TAT) domain (11 amino acids, YGRKKRRQRRR) of the human immunodeficiency virus-1 (HIV-1) TAT protein can efficiently deliver proteins into cells and appears to not be limited by the size of the fusion protein [ 26 ]. (biomedcentral.com)
- As shown in Figure 1, proteins that serve as markers for cellular fractionation, such as nuclear marker RNA pol II and cytoplasmic marker alpha Tubulin, were only detected in their respective cellular fractions and in whole-cell extracts. (activemotif.com)
- To evaluate the efficiency of cellular fractionation, 20 μg of nuclear (Nu), cytoplasmic (Cy) and whole-cell (WC) extracts, along with nuclear pellet collected during nuclear extraction using the Nuclear Extract Kit were analyzed by Western blot using antibodies against various specific proteins. (activemotif.com)
- Magnetic Beads are superparamagnetic, non-aggregating iron oxide particles (or microspheres ) for capturing or purifying targets such as proteins, antigens, antibodies, DNA/RNA, cells, and more. (raybiotech.com)
- These proteins are rich in antibodies and clotting factors and can be separated through a process called fractionation. (hemarusplasma.us)
Tumor cells4
- Circulating tumor cells (CTCs) are found in the blood of cancer patients and can form new tumors. (ox.ac.uk)
- 1 Casodex, an antiandrogen used in prostate cancer therapy, is designed to reduce tumor size by interfering with normal androgen receptor (AR)-mediated processes that ensure prostate cell survival and by triggering tumor cells to undergo apoptosis. (nature.com)
- The so-called fractionation of radiation is thought to help preferentially kill tumor cells and minimize chronic tissue damage," Kishan explained. (medscape.com)
- In MDA-MB-231 cells, BTK-C expression confers modest increases in proliferation and glucose uptake rates compared to BTK-A. Conclusion: Palmitoylation affects localization and regulation of BTK-C in epithelial tumor cells where it functions as an important survival factor. (iiarjournals.org)
Subcellular2
- Materials and Methods: Site-directed mutagenesis, acylation assays and expression studies in cancer cell lines were used to determine the effects that the BTK-C first exon sequence has on kinase activity, subcellular localization and cell physiology. (iiarjournals.org)
- Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS . (bvsalud.org)
Efficient cell lysis2
- RIPA buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with immunoreactivity and biological activity. (sigmaaldrich.com)
- Learn about methods and technologies for efficient cell lysis, protein extraction and fractionation, and targeted inhibition of unwanted protease and phosphatase activity. (thermofisher.com)
Eukaryotic cells3
- In eukaryotic cells, DNA is found in a) Chromosomes ) Chromatin c) Mitochondria d) a, b, c e) a and b 5. (campuscrosswalk.org)
- The membranes that partition the cytoplasm of eukaryotic cells (endomembrane system) include a) Golgi complex b) Lysosomes c) Endoplasmic reticulum d) Transport vesicles e) All of the above 1. (campuscrosswalk.org)
- An outline -- Preparation of escherichia coli samples for 2-D gel analysis -- Preparing 2-D protein extracts from yeast -- 2-D protein extracts from drosophila melanogaster -- Preparing 2-D protein extracts from caenorhabditis elegans -- Eukaryotic cell labeling and preparation for 2-D -- Differential detergent fractionation of eukaryotic cells. (epa.gov)
Radiotherapy3
- Accelerated fractionation compared to conventional fractionation improves loco regional control in radiotherapy of advanced head and neck cancers: results of the EORTC 22851 randomized trial. (msjonline.org)
- Concurrent radiotherapy and chemo therapy with cisplatin in inoperable squamous cell carcinoma of the head and neck. (msjonline.org)
- Aim: This retrospective review was conducted to compare outcomes using different fractionation schedules for early stage glottic cancer treated with radiotherapy between 1991 and 2003. (edu.au)
Chromatin2
- Proliferating fibroblasts and HeLa cells co-cultured in vitro reciprocally influence growth patterns, protein expression, chromatin features and cell survival. (iiar-anticancer.org)
- Consistent with this notion, we found that PLU-1 is present in the chromatin fraction in biochemical cell fractionation experiments using both somatic and meiotic cells. (ox.ac.uk)
20191
- 14. Yating Qin, Hui Peng, Xiwen He, Wenyou Li* , Yukui Zhang, Highly Effective Drug Delivery and Cell Imaging Using Fluorescent Double-Imprinted Nanoparticles by Targeting Recognition of the Epitope of Membrane Protein, Analytical Chemistry , 2019, 91: 12696-12703. (dicp.ac.cn)
Localization1
- We have performed growth tests, membrane localization following cell fractionation, and glycerol accumulation measurements as well as an investigation of the osmotic stress response. (aston.ac.uk)
Mammalian cells2
- CytoBuster™ protein extraction reagent is available for lysis and protein extraction from insect and mammalian cells. (sigmaaldrich.com)
- Fractionation of nucleic acids from mammalian cells in culture by polylysine-Kieselguhr column chromatography. (iiar-anticancer.org)
Lysis2
- A variety of reagents and enzymes for cell lysis and protein extraction provide an array of options tailored for specific cell types and application needs. (sigmaaldrich.com)
- It is used as a non-ionic detergent for cell lysis and for nuclei isolation & cell fractionation. (orchemproducts.in)
Cellular6
- Traditionally, B cells have been believed to be the lymphocytes responsible for antibody production via maturation into plasma cells (ie, humoral immunity), and T cells have been believed to be the lymphocytes responsible for killing other cells or organisms (ie, cellular immunity). (medscape.com)
- The relationship among AE1, AE2 and p16 in GC cells was characterized by molecular and cellular experiments. (biomedcentral.com)
- To demonstrate the efficiency of cellular fractionation using the Nuclear Extract Kit, nuclear, cytoplasmic, and whole-cell extracts were prepared from HeLa cell samples. (activemotif.com)
- Multiple and different cellular activities have already been related to the PP-InsPs, but an over-arching hypothesis sights them as performing as an user interface between energy fat burning capacity and cell-signaling [3,5,6]. (immune-source.com)
- Our work unveils a crucial role for TFIIIA in transcriptional regulation of a cellular RIG-I agonist and shows that GTF3A genetic defects lead to impaired cell-intrinsic anti-HSV-1 responses and can predispose to HSE. (bvsalud.org)
- Exploration of mutations and analysis of cellular changes related to lymphocyte defects and immune dysregulation has fueled the development of novel treatment options for some primary T-cell disorders that might otherwise by fatal. (medscape.com)
Epithelial2
- Active cell death, or apoptosis, plays a central role in maintaining tissue homeostasis and proper disposal of damaged or excess cells, including the epithelial cells of the prostate after castration or administration of antiandrogens. (nature.com)
- Nonrandom association of acrocentric chromosomes in human epithelial cells. (iiar-anticancer.org)
Conventional1
- Conventional therapy appropriate for the immunologically healthy host is administered, although patients with T-cell defects characteristically have more prolonged and severe clinical courses. (medscape.com)
Cytoplasmic6
- Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors. (ox.ac.uk)
- The Nuclear Extract Kit isolates high-quality nuclear, cytoplasmic or whole-cell extract from mammalian cell or tissue samples. (activemotif.com)
- The Nuclear Extract Kit works with fresh or frozen cell and tissue samples and yields protein amounts of approximately 0.5 to 1 mg from cytoplasmic extract (at 1-2 mg/ml concentration), 150 to 250 μg from nuclear extract (at 3-5 mg/ml concentration), 1.2 to 2.4 mg from whole cell extract (at 4-8 mg/ml concentration) from 8.8 x 10 6 cells. (activemotif.com)
- To demonstrate the quality of the materials and protocol of the Nuclear Extract Kit, nuclear and cytoplasmic extracts were prepared from HeLa cell samples that were either unstimulated or stimulated with TNF-α for 30 minutes. (activemotif.com)
- NFκB activation was assayed with the TransAM ® NFκB p50 Kit using increasing amounts of nuclear and cytoplasmic extracts isolated from unstimulated and stimulated HeLa cells using the Nuclear Extract Kit. (activemotif.com)
- Active Motif also offers a number of ready-to-use nuclear, cytoplasmic and whole-cell extracts . (activemotif.com)
Concurrent1
- Aim of the study was to compare the response of altered fractionation schedule with concurrent chemo-radiation in patients with primary and the nodal disease. (msjonline.org)
Nucleus2
- Using cell fractionation and immunoprecipitation, we further demonstrated that Nrf2 and Keap1 were both detected in the cytoplasm and nucleus. (cdc.gov)
- Because activated NFκB translocates into the nucleus, only nuclear extract from stimulated cells should contain activated NFκB. (activemotif.com)
HeLa4
- Interactions between human fibroblastsand HeLa cells in vitro. (iiar-anticancer.org)
- Cytogenetical changes during early stages of developmentof methotrexate resistance in HeLa cells. (iiar-anticancer.org)
- We confirmed that the TAT-S3 probe localized in the mitochondria, without permeabilization, and fluoresced in H 2 O 2 -treated HeLa cells and zebrafish embryos. (biomedcentral.com)
- De Souza CEA, Andrade Pires AR, Cardoso CR, Carlos RM, Cadena SMSC, Acco A (2020) Antineoplastic activity of a novel ruthenium complex against human hepatocellular carcinoma (HepG2) and human cervical adenocarcinoma (HeLa) cells. (mitophysiology.org)
Genetic4
- The cell theory states that a) New cells come from preexisting cells b) All cells are descended from ancient cells ) Cells divide d) Cells contain genetic material e) None of the above 15. (campuscrosswalk.org)
- Genetic experiments using mouse embryonic fibroblast (MEF) cells derived from wild type or Nrf2 knockout mice revealed that the cells lacking Nrf2 exhibited markedly increased production of ROS and sensitivity to Cd cytotoxicity. (cdc.gov)
- Vectors carry genetic instructions to specific cells. (biospace.com)
- These genetic instructions remain in the target cells, but never actually become a part of a person's own DNA. (biospace.com)
Nuclei1
- Our goal is to facilitate the use of single cell/nuclei sequencing technologies for all researchers. (ki.se)
Mitochondria4
- Overexpression of Bcl-2 in LNCaP cells attenuates the induction of cell death by TNF- α but not Casodex, suggesting that mitochondria depolarization is not required for the induction of cell death by Casodex. (nature.com)
- The role of mitochondria in cell metabolism and survival is controlled by calcium signals that are commonly transmitted at the close associations between mitochondria and endoplasmic reticulum (ER). (rupress.org)
- However, the physical linkage of the ER-mitochondria interface and its relevance for cell function remains elusive. (rupress.org)
- Interestingly, confocal immunocytochemistry and cell fractionation studies indicate that Sab is associated with mitochondria, where it co-localizes with a fraction of active JNK. (portlandpress.com)
Chromatography3
- We report on a new integrated platform for automated harvesting of whole unclarified cell-culture broths, followed by in-line tandem affinity-capture, pH neutralization and size-exclusion chromatography of recombinant antibodies expressed transiently in mammalian human embryonic kidney 293T-cells at the 1-L scale. (cyberleninka.org)
- The system consists of two bench-top chromatography instruments connected to a central unit with eight disposable filtration devices used for loading and filtering the cell cultures. (cyberleninka.org)
- This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. (rockland.com)
Plasma8
- For the automated processing of whole blood into separate components of plasma, buffy layer or red blood cells for downstream analysis or biobanking. (perkinelmer.com)
- The JANUS ® G3 Blood iQ ™ Workstation fully automates the process of separating centrifuged whole blood into components of plasma, buffy coat and red blood cells. (perkinelmer.com)
- The Expert Committee recommended adoption of guidelines for DNA vaccines and rotavirus vaccines, and advised that recommendations should be adopted for whole-cell, pertussis vaccine, human plasma for fractionation and rabies vaccine. (who.int)
- Fresh whole blood has long been thought of as the criterion standard for transfusion, but the advent of whole blood fractionation techniques subsequent to World War II provided a means of more efficient use of the various components (i.e., packed red blood cells [PRBCs], fresh frozen plasma [FFP], individual factor concentrates, platelet concentrates, cryoprecipitate). (medscape.com)
- Developing countries thus have to create their own sustainable supplies of blood-derived products using blood plasma collected by their own establishments and from their own populations, even if fractionation is done in developed countries. (who.int)
- Plasma is yellow in color and is the liquid portion of the blood in which blood cells are suspended. (hemarusplasma.us)
- However, the plasma is separated from the red blood cells and the red cells are returned to the donor. (hemarusplasma.us)
- WHO also supported Somalia in the establishment of a national blood centre and Egypt in initiating mechanisms for the use of plasma for fractionation. (who.int)
White Blood3
- Separation of cancer cells from white blood cells by pinched flow fractionation. (ox.ac.uk)
- In this paper, the microfluidic size-separation technique pinched flow fractionation (PFF) is used to separate cancer cells from white blood cells (WBCs). (ox.ac.uk)
- The complete blood count (CBC) is a group of tests that evaluate your overall health that is made up of a group of cells within your blood, including red blood cells (RBC's), white blood cells (WBC's), platelets (PLTs). (arcpointlabs.com)
Electron1
- The ability of a microscope to reveal fine detail is known as a) Magnification b) Resolving power c) Cell fractionation d) Scanning electron microscopy e) Phase contrast 3. (campuscrosswalk.org)
Inhibition1
- These compounds showed immunosuppressant effect in the T-cell proliferation assay with IC 50 values between 31.2 to 2.7 μg/mL, while the IC 50 values for ROS inhibition, representing anti-inflammatory effect, were in the range of 25.6 to 2.0 μg/mL. (biomedcentral.com)
Soybean2
- For example, rice bran oil refining machine, palm oil refining/refinery machine, palm oil fractionation machinery, soybean oil refining machine, sunflower oil refining machine, cotton oil refining/winterization machinery, corn oil refinery machine/winterization line, peanut oil refinery machine and others crude cooking oil refinery line. (sozailink.com)
- d.flaking process for Soybean Oil Plant: rolling raw material into flakes, and the purpose is to increae surface area, destroy the soybean cell tissue, shorten the way of oil out from cake. (ipras.org)
Reagents2
- In 2015, the single cell field was revolutionized by the introduction of droplet sequencing, in other words the encapsulation of cells and reagents into small droplets for library preparation. (ki.se)
- PopCulture ® reagents enable protein extraction from cells directly in the culture medium with no centrifugation required. (sigmaaldrich.com)
Digestion2
- A single cell suspension from C57BL/6 mouse brain was prepared using Trypsin digestion and 70/37/30% percoll gradient to isolate CX3CR1 + microglia using the MojoSort™ Mouse CX3CR1 Selection Kit. (biolegend.com)
- Tissue enzymatic digestion, followed by percoll fractionation, is recommended. (biolegend.com)
20161
- The team has abundant experience with single cell technologies since the inception of SICOF in 2016 as an in-house facility for the Integrated Cardio Metabolic Institute (ICMC). (ki.se)
Assays3
- Extracts prepared using the Nuclear Extract Kit can be used with Active Motif's TransAM ® Kits , or in other procedures that require cell extracts, like gelshift assays, Western blots, DNA footprinting, as a starting point for protein purification for binding or activity assays or or other analysis techniques. (activemotif.com)
- 1997. In vitro genotoxic effects of hard metal particles assessed by alkaline single cell gel and elution assays. (cdc.gov)
- The immunomodulatory, and anti-inflammatory activities and cytotoxicity of compounds 1 - 7 were evaluated by observing their effects on T-cell proliferation, reactive oxygen species (ROS) production, and normal cell growth in MTT assays, respectively. (biomedcentral.com)
Toxicity2
- Grades 2 and 3 late genitourinary (GU) toxicity was higher with the higher fractionation dose, at 14.6%, compared with 8.2% for the lower fractionation dose, but there were no differences in grade 2 gastrointestinal (GI) toxicity between the two schedules. (medscape.com)
- Conclusion: The two RT fractionation schedules showed equal efficacy in terms of symptoms relief, radiological response of the primary thoracic tumor, respiratory functions and toxicity. (scirp.org)
Degradation1
- In this study, we revealed that p16 binds not only AE1 but also AE2, and that the formation of the AE1/p16 complex accounted for the enhanced degradation of AE2 in poorly differentiated GC cells. (biomedcentral.com)
Compartment1
- Once cells are enriched in the upper compartment, they can be exhausted by closing the outlet of the lower compartment and opening the outlet of the upper compartment. (microfluidic-chipshop.com)
Flow cytometry3
- The MedH Flow Cytometry core facility for single cell sorting (FACS). (ki.se)
- Although we do offer single cell sorting support, we would like to refer you in the first place to the MedH Flow Cytometry Core Facility , which is cheaper and has more dedicated support for your flow cytometry and single cell sorts. (ki.se)
- For the patients in the clinical trials, naïve T-cell levels were measured using flow cytometry at 6, 12, and 24 months after implantation. (medscape.com)
Acute1
- Establishment and characterization of a B-cell line from a patient with acute lymphoblastic leukemia. (iiar-anticancer.org)
Endothelial cells3
- Receptor-mediated endocytosis of VEGF-A in rat liver sinusoidal endothelial cells. (uio.no)
- PURPOSE: Fuchs endothelial corneal dystrophy (FECD) is a progressive corneal disease that impacts the structure and stiffness of the Descemet's membrane (DM), the substratum for corneal endothelial cells (CECs). (bvsalud.org)
- METHODS: In this study, we investigated the effect of AA, TGF-ß1 and TGF-ß3 on the deposition, ultrastructure, stiffness, and composition of the extracellular matrix (ECM) secreted by primary bovine corneal endothelial cells (BCECs). (bvsalud.org)
Survival5
- Antiandrogens such as Casodex (Bicalutamide) are designed to treat advance stage prostate cancer by interfering with androgen receptor-mediated cell survival and by initiating cell death. (nature.com)
- Taken together, these data support the hypothesis that Casodex induces cell death by a pathway that is independent of changes in ΔΨ m and Bcl-2 actions and results in an extended lag phase of cell survival that may promote the induction of an invasive phenotype after treatment. (nature.com)
- We previously identified an isoform of the PH domaincontaining kinase, Bruton's tyrosine kinase (BTK), in an RNAi kinome screen as a critical survival factor for breast cancer cells ( 6 ). (iiarjournals.org)
- Opinions are embraced and defended, but transfusion of red blood cells has not reliably demonstrated increased survival, other than in 2 specific populations, as follows: (1) those with active hemorrhage, and (2) those with active cardiac ischemia. (medscape.com)
- Using a metastatic Lewis lung carcinoma (LLC) cell model, we show that GsdmD knockout (GsdmD-/-) mice form significantly fewer cancer foci in lungs, exhibit markedly decreased lung cancer metastasis, and show a significant â ¼50% increase in median survival rate. (bvsalud.org)
Vitro6
- Cell-cell interactions, stromal cells, drug resistance, in vitro evaluation of anticancer agents, mechanisms of invasion and metastasis. (iiar-anticancer.org)
- Fibroblasts against cancer cells in vitro. (iiar-anticancer.org)
- Interactions between human lymphoblastoid cells and human fibroblast feeder layers in vitro. (iiar-anticancer.org)
- In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
- 2012) reported on human skin penetration of cobalt nanoparticles through intact and damaged skin suggesting that Co applied as NPs is able to penetrate the human skin in an in-vitro diffusion cell system. (malvernpanalytical.com)
- During current study, compound 1 was found to exhibit significant immunomodulatory and anti-inflammatory activities in vitro in T-cell proliferation and reactive oxygen species (ROS) production assay, respectively. (biomedcentral.com)
Tumors1
- Double minutes in fibroblast-like cells isolated from human tumors. (iiar-anticancer.org)
Membrane3
- In cells treated with TNF- α , this is accompanied by the loss of mitochondrial membrane potential (ΔΨ m ) and cell adhesion. (nature.com)
- Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures. (mpg.de)
- Cells are filtered by a membrane with determined pore size. (microfluidic-chipshop.com)
Qualifier1
- Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. (yeastgenome.org)
Cancer cells5
- The separation is challenged by the size overlap between cancer cells and the 10(6) times more abundant WBCs. (ox.ac.uk)
- Treatment of androgen sensitive, non-metastatic LNCaP human prostate cancer cells with 0-100 μ M Casodex or 0-10 ng/ml TNF- α induces cell death in 20-60% of the cells by 48 h in a dose-dependent manner. (nature.com)
- An increasingly attractive strategy is to primarily enrich extracellular vesicles (EVs) released from cancer cells in an accelerated manner compared to normal cells. (mdpi.com)
- Teaming Up for Trouble: Cancer Cells, Transforming Growth Factor-β1 Signaling and the Epigenetic Corruption of Stromal Naïve Fibroblasts. (iiar-anticancer.org)
- The BTK-C isoform identified in breast and prostate cancer cells ( 7 ) is similar to the original BTK isoform, which we refer to as BTK-A for clarity, but contains an alternative first exon encoding parts of a 34 amino acid amino-terminal extension ( 6 ). (iiarjournals.org)
Embryonic1
- Viral infection of embryonic day 7 chick retinal ganglion cells with mutated NgR demonstrates that the NgR C-terminal domain is required for inhibitory signaling but not ligand binding. (jneurosci.org)
Serum2
- Insect PopCulture ® reagent lyses insect cells directly in serum-free medium and is ideal for expression screening of many small samples. (sigmaaldrich.com)
- Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. (jnephrol.com)
Dose3
- Arm A was altered fractionation schedule where in patients received 6 fractions per week to a total dose of 6600 cGy in 33 fractions. (msjonline.org)
- In MEF cells with wild type Nrf2 and mouse hepa 1c1c7 cells, Cd increased the Nrf2 protein level but not the Nrf2 mRNA level dose-dependently. (cdc.gov)
- Par exemple, seuls 6,1 % des répondants étaient capables de décrire le principe ALARA et 98,2 % ignoraient qu'il n'existait pas de seuil en dessous duquel une dose est sans danger, selon les recommandations internationales. (who.int)
Transformation1
- In this manuscript, we report for the first time the microbial transformation of a synthetic anabolic steroidal drug, oxymetholone, by fungal cell cultures. (biomedcentral.com)
Techniques1
- This course introduces cell growth and fractionation techniques, and topics include fermentation theory and application and cell harvesting. (cccc.edu)
Markers1
- The core biopsy revealed a spindle cells malignant tumor, negative for pan cytokeratine and most of immuno-histochemical (IH) markers, suggesting sarcoma, but requiring study of surgical specimen to exclude Metaplastic Carcinoma. (bvsalud.org)
Buffer1
- 10 µl of antibody cocktail for 1 X 10 6 cells in 100 µl of buffer. (biolegend.com)
Separation1
- The size overlap prevents high efficiency separation, however we demonstrate that cell deformability can be exploited in PFF devices to gain higher efficiencies than expected from the size distribution of the cells. (ox.ac.uk)
Downstream1
- While both TNF- α and Casodex-induced release of cytochrome c in LNCaP cell is predominantely associated with the translocation and cleavage of Bax, our data also suggest that Casodex induces cell death by acting on components downstream of decline of ΔΨ m and upstream of cytochrome c release. (nature.com)
Cultures1
- The revised recommendations, therefore, provide specifications for rabies vaccines produced in cell cultures or purified from embryonated eggs since these vaccines are safe and have dramatically decreased the number of human deaths throughout the world, most notably in countries where canine rabies is endemic. (who.int)
Biol5
- J Cell Biol (2006) 174 (7): 915-921. (rupress.org)
- Biol Cell 59(1):69-77, 1987. (iiar-anticancer.org)
- Delinassios JG, Kottaridis SD and Garas J. Exp Cell Biol 51(4):201-9, 1983. (iiar-anticancer.org)
- Biol Cell 50(1):9-16, 1984. (iiar-anticancer.org)
- Exp Cell Biol 49(4):177-84, 1981. (iiar-anticancer.org)
Yeast cell2
- The main function of Fps1p seems to be the efflux of glycerol in the adaptation of the yeast cell to lower external osmolarity. (aston.ac.uk)
- Lyticase enzyme: enzymatically cleaves yeast cell wall β-glucan layers allowing for access to cell contents. (sigmaaldrich.com)
Oxidative stress1
- Oxidative stress and transforming growth factor-ß (TGF-ß) pathways have been implicated in endothelial cell loss and endothelial to mesenchymal transition of CECs in FECD. (bvsalud.org)
Pathway2
- 7 The death receptor-mediated pathway is activated upon ligand binding of cell surface death receptors such as tumor necrosis factor- α (TNF- α ), initiating ligand-induced receptor trimerization and the formation of death-inducing signaling complex (DISC). (nature.com)
- Based on previous studies in bacterial cells, base excision repair (BER) has been established as the major pathway for the removal of this lesion [ 8 ]. (biomedcentral.com)