Cell extracts. Medical search

Impaired translesion synthesis in xeroderma pigmentosum variant extracts. (1/1728)

Xeroderma pigmentosum variant (XPV) cells are characterized by a cellular defect in the ability to synthesize intact daughter DNA strands on damaged templates. Molecular mechanisms that facilitate replication fork progression on damaged DNA in normal cells are not well defined. In this study, we used single-stranded plasmid molecules containing a single N-2-acetylaminofluorene (AAF) adduct to analyze translesion synthesis (TLS) catalyzed by extracts of either normal or XPV primary skin fibroblasts. In one of the substrates, the single AAF adduct was located at the 3' end of a run of three guanines that was previously shown to induce deletion of one G by a slippage mechanism. Primer extension reactions performed by normal cellular extracts from four different individuals produced the same distinct pattern of TLS, with over 80% of the products resulting from the elongation of a slipped intermediate and the remaining 20% resulting from a nonslipped intermediate. In contrast, with cellular extracts from five different XPV patients, the TLS reaction was strongly reduced, yielding only low amounts of TLS via the nonslipped intermediate. With our second substrate, in which the AAF adduct was located at the first G in the run, thus preventing slippage from occurring, we confirmed that normal extracts were able to perform TLS 10-fold more efficiently than XPV extracts. These data demonstrate unequivocally that the defect in XPV cells resides in translesion synthesis independently of the slippage process. (+info)

Mutations of oncoprotein 18/stathmin identify tubulin-directed regulatory activities distinct from tubulin association. (2/1728)

Oncoprotein 18/stathmin (Op18) is a recently identified phosphorylation-responsive regulator of the microtubule (MT) system. It was originally proposed that Op18 specifically regulates dynamic properties of MTs by associating with tubulin, but it has subsequently been proposed that Op18 acts simply by sequestering of tubulin heterodimers. We have dissected the mechanistic action of Op18 by generation of two distinct classes of mutants. One class has interruptions of the heptad repeats of a potential coiled-coil region of Op18, and the other involves substitution at all four phosphorylation sites with negatively charged Glu residues. Both types of mutation result in Op18 proteins with a limited decrease in tubulin complex formation. However, the MT-destabilizing activities of the coiled-coil mutants are more severely reduced in transfected leukemia cells than those of the Glu-substituted Op18 derivative, providing evidence for tubulin-directed regulatory activities distinct from tubulin complex formation. Analysis of Op18-mediated regulation of tubulin GTPase activity and taxol-promoted tubulin polymerization showed that while wild-type and Glu-substituted Op18 derivatives are active, the coiled-coil mutants are essentially inactive. This suggests that Op18-tubulin contact involves structural motifs that deliver a signal of regulatory importance to the MT system. (+info)

Regulation of human hsp90alpha gene expression. (3/1728)

Mammalian HSP90alpha and HSP90beta are encoded by two individual genes. On the basis of the upstream sequences of the human hsp90alpha gene, GenBank accession number U25822, we have constructed CAT reporter plasmids driven by individual fragments of the hsp90alpha gene. We found that (1) the proximal heat shock element complex located at -96/-60 enhances hsp90alpha promoter expression; (2) heat shock induction depends upon the coexistence of distal heat shock element at -1031/-1022 and the proximal heat shock element complex of the hsp90alpha gene; (3) unlike hsp90beta, downstream sequences of the transcription start site inhibit hsp90alpha expression. We conclude that the regulatory mechanisms for the expression of hsp90alpha and hsp90beta genes are different. (+info)

unr, a cellular cytoplasmic RNA-binding protein with five cold-shock domains, is required for internal initiation of translation of human rhinovirus RNA. (4/1728)

Initiation of translation of the animal picornavirus RNAs occurs via a mechanism of direct ribosome entry, which requires a segment of the 5' UTR of the RNA, known as the internal ribosome entry site (IRES). In addition, translation of the enterovirus and rhinovirus (HRV) subgroups requires cellular trans-acting factors that are absent from, or limiting in rabbit reticulocytes, but are more abundant in HeLa cell extracts. It has been shown previously that HeLa cells contain two separable activities, each of which independently stimulates HRV IRES-dependent translation when used to supplement reticulocyte lysate; one of these activities was identified as polypyrimidine tract-binding protein (PTB). Here, the purification of the second activity is achieved by use of an RNA-affinity column based on the HRV 5' UTR. It comprises two components: a 38-kD protein (p38), which is a novel member of the GH-WD repeat protein family and has no intrinsic RNA-binding activity; and a 96- to 97-kD protein doublet, which was identified as unr, an RNA-binding protein with five cold-shock domains. Coimmunoprecipitation with antibodies against either protein shows that the two proteins interact with each other, and thus p38 is named unrip (unr-interacting protein). Recombinant unr acts synergistically with recombinant PTB to stimulate translation dependent on the rhinovirus IRES. In contrast, unr did not significantly augment the PTB-dependent stimulation of poliovirus IRES activity. (+info)

Fertilization, embryonic development, and offspring from mouse eggs injected with round spermatids combined with Ca2+ oscillation-inducing sperm factor. (5/1728)

Round spermatids, precursor male gametes, are known to possess the potential to achieve fertilization and embryonic development when injected into eggs. However, injection of spermatids alone seldom activates eggs in the mouse, as spermatids by themselves cannot induce an increase in intracellular Ca2+, a prerequisite for egg activation. We injected a mouse round spermatid into an egg simultaneously with partially purified sperm factor from differentiated hamster spermatozoa. The combined injection produced repetitive Ca2+ increases (Ca2+ oscillations) lasting for at least 4 h as observed at fertilization, and induced activation in 92% of eggs. This method provided 75% fertilization success associated with male and female pronucleus formation and development to 2-cell embryos, while only 7% of eggs were fertilized by injection of a spermatid alone. Of the 2-cell embryos, approximately 50% developed to blastocysts during 5 days of culture in vitro, while no blastocysts were obtained following injection of sperm factor alone. Furthermore, the 2-cell embryos, that were created by spermatids and sperm factor and transplanted into foster mothers, developed into normal offspring, although the percentage was only 22%. All infants grew into healthy adults carrying normal chromosomes. The sperm factor served as a complementary factor for successful fertilization by round spermatid injection. (+info)

Discrete regional distributions suggest diverse functional roles of calcium channel alpha1 subunits in sperm. (6/1728)

The Ca channels of male germ-line cells are partially characterized, but the molecular properties and subcellular localization of the Ca channels of mature sperm are unknown. Here, we probe rodent sperm with anti-peptide antibodies directed to cytosolic domains of cloned rat brain alpha1A, alpha1C, and alpha1E Ca channel subunits. Each recognizes a 200- to 245-kDa band on immunoblots of whole rat sperm extracts. A smaller ( approximately 110-kDa) alpha1C band also is detected. Confocal fluorescence images of mouse sperm show characteristic patterns of punctate alpha1A-, alpha1C-, and alpha1E-immunoreactivity. For alpha1A, the puncta are larger, less numerous, and more variable in distribution than for alpha1C and alpha1E. They are absent from the acrosomal crescent, but are present elsewhere over the sperm head, often at the apical tip and equatorial segment. They also are found at irregular intervals along both the midpiece and the principal piece of the flagellum. For alpha1C and alpha1E, puncta are dense along dorsal and ventral aspects of the acrosomal cap. For alpha1E but not alpha1C, the remainder of the acrosomal region also is labeled. Neither is found in the postacrosomal region or on the midpiece. Puncta of alpha1C and alpha1E occur at regular intervals each in two parallel rows, at the dorsal and ventral aspects of the proximal segment of the flagellar principal piece. The puncta in these arrays become less abundant and intense in the distal flagellum. These results demonstrate that multiple Ca channel proteins are present in mature sperm and are regionally localized in ways that may give them different regulatory roles. (+info)

Defective repair of cisplatin-induced DNA damage caused by reduced XPA protein in testicular germ cell tumours. (7/1728)

Metastatic cancer in adults usually has a fatal outcome. In contrast, advanced testicular germ cell tumours are cured in over 80% of patients using cisplatin-based combination chemotherapy [1]. An understanding of why these cells are sensitive to chemotherapeutic drugs is likely to have implications for the treatment of other types of cancer. Earlier measurements indicate that testis tumour cells are hypersensitive to cisplatin and have a low capacity to remove cisplatin-induced DNA damage from the genome [2] [3]. We have investigated the nucleotide excision repair (NER) capacity of extracts from the well-defined 833K and GCT27 human testis tumour cell lines. Both had a reduced ability to carry out the incision steps of NER in comparison with extracts from known repair-proficient cells. Immunoblotting revealed that the testis tumour cells had normal amounts of most NER proteins, but low levels of the xeroderma pigmentosum group A protein (XPA) and the ERCC1-XPF endonuclease complex. Addition of XPA specifically conferred full NER capacity on the testis tumour extracts. These results show that a low XPA level in the testis tumour cell lines is sufficient to explain their poor ability to remove cisplatin adducts from DNA and might be a major reason for the high cisplatin sensitivity of testis tumours. Targeted inhibition of XPA could sensitise other types of cells and tumours to cisplatin and broaden the usefulness of this chemotherapeutic agent. (+info)

Bcl-2 regulates a caspase-3/caspase-2 apoptotic cascade in cytosolic extracts. (8/1728)

Apoptosis is accompanied by the activation of a number of apoptotic proteases (caspases) which selectively cleave specific cellular substrates. Caspases themselves are zymogens which are activated by proteolysis. It is widely believed that 'initiator' caspases are recruited to and activated within apoptotic signalling complexes, and then cleave and activate downstream 'effector' caspases. While activation of the effector caspase, caspase-3, has indeed been observed as distal to activation of several different initiator caspases, evidence for a further downstream proteolytic cascade is limited. In particular, there is little evidence that cellular levels of caspase-3 that are activated via one pathway are sufficient to cleave and activate other initiator caspases. To address this issue, the ability of caspase-3, activated upon addition to cytosolic extracts of cytochrome c, to cause cleavage of caspase-2 was investigated. It was demonstrated that cleavage of caspase-2 follows, and is dependent upon, activation of caspase-3. Moreover, the activation of both caspases was inhibited by Bcl-2. Together, these data indicate that Bcl-2 can protect cells from apoptosis by acting at a point downstream from release of mitochondrial cytochrome c, thereby preventing a caspase-3 dependent proteolytic cascade. (+info)

The main chapters imported in 2012 were: coffee (75.3% of the total); juices and vegetable extracts (18.9%); nuts (3.5%); ... The main exported chapters consisted of: sounding devices (44.5% of the total); photovoltaic cells (20.4%); centrifugal pumps ( ...

https://en.wikipedia.org/wiki/Rwanda–Spain_relations

The structure and properties of levan, a polymer of d-fructose produced by cultures and cell-free extracts of Aerobacter ... The structure and properties of levan, a polymer of d-fructose produced by cultures and cell-free extracts of Aerobacter ... 78:401-406, 1958 Xylosyl transfer by asparagus extracts. Feingold, D. S., Neufeld, E. F. and Hassid, W. Z. J. Biol. Chem. 234: ... Interferon production in mice by cell wall mutants of Salmonella typhimurium. Youngner, J. S. and Feingold, D. S. Virology 1: ...

https://en.wikipedia.org/wiki/David_Sidney_Feingold

Cell. 122 (3): 379-91. doi:10.1016/j.cell.2005.06.015. PMC 4401993. PMID 16096058. Glanzer J, Miyashiro KY, Sul JY, Barrett L, ... Jamison SF, Crow A, Garcia-Blanco MA (October 1992). "The spliceosome assembly pathway in mammalian extracts". Molecular and ... doi:10.1016/j.cell.2017.04.033. PMID 28502770. Schmucker D, Clemens JC, Shu H, Worby CA, Xiao J, Muda M, Dixon JE, Zipursky SL ... Zhang X, Yan C, Hang J, Finci LI, Lei J, Shi Y (May 2017). "An Atomic Structure of the Human Spliceosome". Cell. 169 (5): 918- ...

https://en.wikipedia.org/wiki/Spliceosome

The company extracts cells from live animals. It cultures those cells in stainless steel fermenters, where they reproduce and ... Purdy, Chase (22 January 2020). "A startup says it's building a US pilot plant for cell-based meat". Quartz. Retrieved 27 May ... Future Meat Technologies, or Future Meat for short, is a biotechnology firm which produces cultured meat from chicken cells and ... Future Meat Technologies mainly seeks to supply hardware and cell lines to manufacturers of cultured meat rather than directly ...

https://en.wikipedia.org/wiki/Future_Meat_Technologies

Kornberg T, Gefter ML (1971). "Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II". Proc. ... Kornberg T, Gefter ML (10 September 1972). "Deoxyribonucleic acid synthesis in cell-free extracts. IV. Purification and ...

https://en.wikipedia.org/wiki/Thomas_B._Kornberg

1 Preparation and properties of cell-free extracts". J. Biol. Chem. 240 (12): 4669-74. PMID 5846987. Bradbeer C (1965). "The ...

https://en.wikipedia.org/wiki/Ethanolamine_ammonia-lyase

Bloodroot extracts have also been promoted by some dietary supplement companies as a treatment or cure for cancer, but the U.S ... Sanguinarine kills animal cells by blocking the action of Na+/K+-ATPase transmembrane proteins. As a result, applying bloodroot ... Bloodroot and its extracts are thus considered escharotic. Although applying escharotic agents (including bloodroot) to the ... An overdose of bloodroot extract can cause vomiting and loss of consciousness. Comparing the biosynthesis of morphine and ...

https://en.wikipedia.org/wiki/Sanguinaria

"Methenyl-tetrahydromethanopterin cyclohydrolase in cell extracts of Methanobacterium". Arch. Biochem. Biophys. 242 (2): 430-9. ...

https://en.wikipedia.org/wiki/Methenyltetrahydromethanopterin_cyclohydrolase

... extract against two types of herpes simplex viruses in cell culture. Die Pharmazie-An International Journal of Pharmaceutical ... Extracting seed from the fruit involves cleaning the flesh off by placing fruits in a macerator, blender, or by soaking the ... Rayne, S., & Mazza, G. (2007). Biological activities of extracts from sumac (Rhus spp.): a review. Nature Precedings, 1-1. ... ovata extract have been proven to have antibacterial properties that impede the growth of gram-positive bacteria like ...

https://en.wikipedia.org/wiki/Rhus_ovata

Philpott A, Leno GH (May 1992). "Nucleoplasmin remodels sperm chromatin in Xenopus egg extracts". Cell. 69 (5): 759-767. doi: ... Seminars in Cell Biology. 1 (1): 11-17. PMID 1983266. Rice P, Garduño R, Itoh T, Katagiri C, Ausio J (June 1995). " ... Cell. 30 (2): 449-458. doi:10.1016/0092-8674(82)90242-2. PMID 6814762. Tejun S, Yaozhou Z (2007). "Nucleoplasmin, an Important ...

https://en.wikipedia.org/wiki/Nucleoplasmin

Cell. Biochem. 5 (1-2): 55-63. doi:10.1007/BF01874172. PMID 4279328. Kalckar, H. M. Biological phosphorylations: development of ... Kalckar, H. M. (1939). "The Nature of Phosphoric Esters Formed in Kidney Extracts". Biochemical Journal. 33 (5): 631-641. doi: ... which they purified from muscle extracts. Further work on nucleotide metabolism allowed him to identify nucleoside ...

https://en.wikipedia.org/wiki/Herman_Kalckar

Hydrolysis of nucleic acids in crude cell-free extracts. Sequencing of RNA. Preparation of rabbit reticulocyte lysates. Studies ... A common source is E.coli cells carrying a cloned nuc gene encoding Staphylococcus aureus extracellular nuclease (micrococcal ...

https://en.wikipedia.org/wiki/Micrococcal_nuclease

Studies have shown that its extracts have renal effects. It has also been seen that in rats, such extracts cause an increase in ... The surface cells are loosely arranged. The number of chromosomes is 16, which is 2n = 8. It has been said that the species of ... "Effects of hydroalcoholic extracts of Portulaca pilosa and Achyrocline satureioides on urinary sodium and potassium excretion ...

https://en.wikipedia.org/wiki/Portulaca_pilosa

"Toxic effects of Karenia mikimotoi extracts on mammalian cells". Chinese Journal of Oceanology and Limnology. 29 (4): 860-868. ...

https://en.wikipedia.org/wiki/Karenia_mikimotoi

The antigens are either from cell extracts or recombinant. Blood serum is incubated in the wells of the plate and is washed out ... T-cells and B-cells) and antigen presenting cells. These cells coordinate an immune response upon the detection of foreign ... the cell line was contaminated and displaced by HeLa cells, and has now been identified as actually HeLa cells. They are ... They are termed ENA because they can be extracted from the cell nucleus with saline. The ENAs consist of ribonucleoproteins and ...

https://en.wikipedia.org/wiki/Antinuclear_antibody

One study showed that extracts of H. stigonocarpa suppressed cell division in onions, and this may imply that it contains some ... Fabaceae) on the cell cycle of Allium cepa L." Anais da Academia Brasileira de Ciências. 86 (3): 1147-1150. doi:10.1590/0001- ... Lacerda, Lourran P.; Malaquias, Geiz; Peron, Ana Paula (2014). "Antiproliferative action of aqueous extracts of Hymenaea ...

https://en.wikipedia.org/wiki/Hymenaea_stigonocarpa

Research in the early 20th century focused on extracting crude extracts and reconciling some of the conflicting views on ... Sugawara, I (1982). "Polysaccharides with sulfate groups are human T-cell mitogens and murine polyclonal B-cell activators ( ... Methods of extracts and isolation of fucoidan from brown seaweeds were determined on laboratory scale by Black et al. at the ... Fucoidan extracts are utilised in a range of therapeutic health care preparations, being incorporated as high value ingredients ...

https://en.wikipedia.org/wiki/Fucoidan

Knysh O, Martynov A (2020). "Lactobacillus reuteri cell-free extracts against antibiotic-resistant bacteria". Zaporozhye ... Cell. 165 (7): 1762-1775. doi:10.1016/j.cell.2016.06.001. PMC 5102250. PMID 27315483. Wang XD, Soltesz V, Molin G, Andersson R ...

https://en.wikipedia.org/wiki/Limosilactobacillus_reuteri

Fermentation is another process by which cells can extract energy from glucose. It is not a form of cellular respiration, but ... This phenomenon is called the Warburg effect and is found primarily in cancer cells. Muscles cells under great exertion will ... Generally, lactic acid fermentation occurs only when aerobic cells are lacking oxygen. However, some aerobic mammalian cells ... CO2 is excreted from the cell via diffusion into the blood stream, where it is transported in three ways: Up to 7% is dissolved ...

https://en.wikipedia.org/wiki/Cellular_waste_product

... is typically extracted by ethanol precipitation from liquid culture or by alkaline treatment from the cell wall. Once extracted ... Cell Microbiol 12, 405-410 Robinet, P., Baychelier, F., Fontaine, T., Picard, C., Debre, P., Vieillard, V., Latge, J.P., and ... Bardalaye, P.C., and Nordin, J.H. (1976). Galactosaminogalactan from cell walls of Aspergillus niger. J Bacteriol 125, 655-669 ... Similar to other fungal cell wall polysaccharides, galactosaminogalactan is synthesized by polymerization of nucleotide sugars ...

https://en.wikipedia.org/wiki/Galactosaminogalactan

Through experiments in Xenopus laevis cell-free egg extracts, such model was confirmed as the basis for entry into mitosis. ... Cells with a defective G2-M checkpoint will undergo apoptosis or death after cell division if they enter the M phase before ... September 2002). "Hysteresis drives cell-cycle transitions in Xenopus laevis egg extracts". Proceedings of the National Academy ... The G2-M DNA damage checkpoint is an important cell cycle checkpoint in eukaryotic organisms that ensures that cells don't ...

https://en.wikipedia.org/wiki/G2-M_DNA_damage_checkpoint

Mechanism of action: A monoclonal antibody against HER2/neu (erb-B2). Helps kill breast cancer cells that overexpress HER-2, ... Extracts from some medicinal mushrooms are natural biological response modifiers. Immunotherapy "Biological Response Modifiers ... where targeted therapy often relies on the immune system being used to attack cancer cells) and in treating autoimmune diseases ... "Persistence of Autoreactive IgA-Secreting B Cells Despite Multiple Immunosuppressive Medications Including Rituximab". JAMA ...

https://en.wikipedia.org/wiki/Biological_response_modifier

Chiang C, Knight SG (1961). "L-Arabinose metabolism by cell-free extracts of Penicillium chrysogenum". Biochim. Biophys. Acta. ...

https://en.wikipedia.org/wiki/L-arabinitol_4-dehydrogenase

doi:10.1007/s13752-013-0155-6. Barbieri, M (1979). "Ribosome crystallization in homogenates and cell extracts of chick embryos ... and the Max Planck Institute of Molecular Cell Biology and Genetics in Berlin. Since 1992 he is professor of embryology at the ... in the cytoskeleton and in cell compartments. This adaptor-dependent definition of code has been used by Kühn and Hofmeyr to ...

https://en.wikipedia.org/wiki/Marcello_Barbieri

"Hysteresis drives cell-cycle transitions in Xenopus laevis egg extracts". Proceedings of the National Academy of Sciences of ... Biochemical switches in the cell cycle Dynamical Systems Dynamical systems theory Bifurcation Theory Cell cycle Theoretical ... For instance, egg extracts of Xenopus laevis are driven in and out of mitosis irreversibly by positive feedback in the ... For instance, this is important in contexts where a cell decides whether to commit to a particular pathway; a non-hysteretic ...

https://en.wikipedia.org/wiki/Biological_applications_of_bifurcation_theory

D'Ari L, Barker HA (1985). "p-Cresol formation by cell-free extracts of Clostridium difficile". Arch. Microbiol. 143 (3): 311-2 ...

https://en.wikipedia.org/wiki/4-hydroxyphenylacetate_decarboxylase

"Hysteresis drives cell-cycle transitions in Xenopus laevis egg extracts". Proceedings of the National Academy of Sciences. 100 ... Cell. 122 (4): 565-78. doi:10.1016/j.cell.2005.06.016. PMID 16122424. Cross, Frederick R; Siggia, Eric D (2005). "Shake It, ... and cell fate. These studies helped demonstrate how ultrasensitivity, positive feedback, and bistability can allow cells to ... Ferrell Jr, J. E; Machleder, E. M (1998). "The Biochemical Basis of an All-or-None Cell Fate Switch in Xenopus Oocytes". ...

https://en.wikipedia.org/wiki/James_Ferrell

Hydrocarbon oil is typically not used in cell microfluidic research because it is not compatible with cells and damages cell ... Hydrocarbon oil also extracts organic molecules from the aqueous phase. However, fluorosurfactants with fluorinated tails, for ... Droplets containing cells can be stored off-chip in PTFE tubing for up to several days while maintaining cell viability and ... High-throughput cell culture experiments, for example, testing the behavior of bacteria, finding rare cell types, directed ...

https://en.wikipedia.org/wiki/Droplet-based_microfluidics

"Hysteresis drives cell-cycle transitions in Xenopus laevis egg extracts". Proceedings of the National Academy of Sciences of ... Curiously, G2 phase is not a necessary part of the cell cycle, as some cell types (particularly young Xenopus embryos and some ... G2 phase is a period of rapid cell growth and protein synthesis during which the cell prepares itself for mitosis. ... Finally, the continuation of the cell cycle requires persisting oscillations in cyclin-B/CDK1 activity as the cell and its ...

https://en.wikipedia.org/wiki/G2_phase

Turner AM, Mann NH (1989). "Protein kinase activities in cell-free extracts of Rhodomicrobium vannielii". FEMS Microbiology ... After observation, the cells showed a budding filament formation. R. vannielli is unique as it is the only species of its genus ... Mature R. vannielii cells are typically ovoid to spherical in shape, do not form intracellular sulfur globules, and are about ... The cells are then attached by means of slender branched filaments, with the connecting filaments varying in length, but a ...

https://en.wikipedia.org/wiki/Rhodomicrobium_vannielii

"Cell. 126 (1): 107-120. doi:10.1016/j.cell.2006.05.036. PMID 16839880. S2CID 15006256.. ... Boiling the yeast extract renders all proteins inactive (as it denatures them). The ability of boiled extract plus dialyzed ... All cells contain the enzyme hexokinase, which catalyzes the conversion of glucose that has entered the cell into glucose-6- ... Hexokinase is inhibited by high levels of G6P in the cell. Thus the rate of entry of glucose into cells partially depends on ...

https://en.wikipedia.org/wiki/Glycolysis

These are found in all cell membranes and the membranes of most cell organelles.[2] Phosphatidylcholines are structurally ... In 1849, Adolph Strecker was the first to isolate choline from pig bile.[48][49] In 1852, L. Babo and M. Hirschbrunn extracted ... Choline is stored in the cell membranes and organelles as phospholipids, and inside cells as phosphatidylcholines and ... Acetylcholine is even present in the placenta and may help control cell proliferation and differentiation (increases in cell ...

https://en.m.wikipedia.org/wiki/Choline

"Life-like cells are made of metal". New Scientist. September 14, 2011. Retrieved 2014-05-25.. ... Marine diatoms - carbon-based organisms that extract silicon from sea water, in the form of its oxide (silica) and incorporate ... A hypothetical cell membrane termed an azotosome, capable of functioning in liquid methane in Titan conditions was computer- ... The Cronin group at Glasgow University reported self-assembly of tungsten polyoxometalates into cell-like spheres.[27] By ...

https://en.m.wikipedia.org/wiki/Non-organic_life

Phillips, W. A, Bachmann, T., & Storm, J. F. (2018) Apical Function in Neocortical Pyramidal Cells: A Common Pathway by Which ... Bachmann, T. (2012). Can quality be extracted from quantification of interactions by NBS? Frontiers in Computational ... Bachmann, T. (2015) How a (sub)cellular coincidence detection mechanism featuring layer-5 pyramidal cells may help produce ... Implications forvulnerability testing and neuromodulation of pyramidal cells. Neuroscience Letters, 584, 129-134. ...

https://et.wikipedia.org/wiki/Talis_Bachmanni_publikatsioonide_loend

Cell Biol. Advances in Molecular and Cell Biology. Vol. 14. pp. 29-55. doi:10.1016/S1569-2558(08)60339-2. ISBN 9780762301133. . ... By using in vitro transcription and translation system, targeted and query protein was synthesized by the same extract. The ... The activity of the cell is regulated by extracellular signals. Signal propagation inside and/or along the interior of cells ... Cooper G (2000). The cell : a molecular approach (2nd ed.). Washington DC: ASM Press. ISBN 978-0-87893-106-4. .. [page needed] ...

https://en.wikipedia.org/wiki/Protein–protein_interaction

They are, in effect, released from the postsynaptic cell and act on the presynaptic cell, where the target receptors are ... Once extracted, isolated components can be separated using wiped film vacuum distillation or other distillation techniques.[56] ... "Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1821 (4): 694-705. doi:10.1016/j.bbalip.2012.01.009 ... signaling molecules that are released from one cell and activating the cannabinoid receptors present on other nearby cells. ...

https://en.m.wikipedia.org/wiki/Endocannabinoids

The straightforward solution, which is to extract such a substring at every character position in the text and compute h ... and the hashing function can be interpreted as a partition of that space into a grid of cells. The table is often an array with ... A mid-squares hash code is produced by squaring the input and extracting an appropriate number of middle digits or bits. For ...

https://en.wikipedia.org/wiki/Hash_function

Kimball C, Murlin J. Aqueous extracts of pancreas III. Some precipitation reactions of insulin. J Biol Chem 1923;58:337-348. ... anat/phys/devp/horm/cell noco(d)/cong/tumr, sysi/epon proc, drug (A10/H1/H3/H5) ...

https://ta.wikipedia.org/wiki/குளூக்கொகான்

The ammonia extracts the fluid and destroys eye cells and tissue in minutes. ...

https://en.wikipedia.org/wiki/LPG_carrier

A general blotting procedure[5] starts with extraction of total RNA from a homogenized tissue sample or from cells. Eukaryotic ... and the probe is RNA extracted from a tissue and radioactively labelled. The use of DNA microarrays that have come into ... Molecular Biology of the Cell, 5th ed. Garland Science, Taylor & Francis Group, NY, pp 538-539. ... "Northern blot analysis for detection of RNA in pancreatic cancer cells and tissues". Nature Protocols. 4 (1): 37-43. doi: ...

https://en.wikipedia.org/wiki/Northern_blot

... extract any protocol like https:// local prot = url:match('(https*://).+[ \'\']') -- extract address local addr = '' if prot ... each cell being the first value of the qualifier corresponding to the list in ,quals ... extract the qid in the form 'Qnnn' from the value.unit url -- and then fetch the label from that - or symbol if unitabbr is ... extract any icon local url, icon = txt:match('(.+) (.+)') -- make sure there's at least a space at the end url = (url or ...

https://tl.wikipedia.org/wiki/Module:WikidataIB

Extracts from Blackwood's Edinburgh Magazine, 3 (1818) pp. 519-524. Nineteenth Century Literary Manuscripts, Part 4. Retrieved ... For Summer has o'er-brimm'd their clammy cells. First stanza of "To Autumn",[86]. September 1819. ...

https://en.wikipedia.org/wiki/John_Keats

Single-cell protein, meat alternatives containing protein extract from pure or mixed cultures of algae, yeasts, fungi, or ... Another type of single cell protein-based meat alternative (which does not use fungi however but rather bacteria[18]) is ... The seeds are then treated with solvents such as hexane to extract the oil from them. The oil-free soybean meal is then ... Soy protein isolate is a highly pure form of soy protein with a minimum protein content of 90%. The process of extracting the ...

https://en.wikipedia.org/wiki/Meat_analogue

Voltaire was imprisoned in the Bastille from 16 May 1717 to 15 April 1718 in a windowless cell with ten-foot thick walls.[11] ... http://fmls.oxfordjournals.org/cgi/pdf_extract/I/3/230. பார்த்த நாள்: 28 February 2012. ...

https://ta.wikipedia.org/wiki/வோல்ட்டயர்

... s are also used in the cells of organisms in a process known as cellular respiration, where organic molecules are oxidized ... By the 19th century, gas extracted from coal was being used for street lighting in London. In the 20th and 21st centuries, the ... Conventional diesel is similar to gasoline in that it is a mixture of aliphatic hydrocarbons extracted from petroleum. Kerosene ... Kerosene was also produced during the same period from oil shale and bitumen by heating the rock to extract the oil, which was ...

https://en.m.wikipedia.org/wiki/Fuel

"Extracting a Climate Signal from 169 Glacier Records" (PDF). Science. 308 (5722): 675-677. doi:10.1126/science.1107046. PMID ... "Expansion of the Hadley cell under global warming" (PDF). Geophysical Research Letters. 34: L06805. doi:10.1029/2006GL028443 ...

https://kn.wikipedia.org/wiki/ಜಾಗತಿಕ_ತಾಪಮಾನ_ಏರಿಕೆ

"Pomegranate extract demonstrate a selective estrogen receptor modulator profile in human tumor cell lines and in vivo models of ... Eukaryotic cells respond to damaged DNA by stimulating or impairing G1, S, or G2 phases of the cell cycle to initiate DNA ... Estradiol is produced by action of aromatase mainly in the Leydig cells of the mammalian testis, but also by some germ cells ... Cell. Endocrinol. 265-266: 138-42. doi:10.1016/j.mce.2006.12.010. PMC 1847610. PMID 17222505.. ...

https://en.m.wikipedia.org/wiki/Estradiol

2,0 2,1 2,2 2,3 Vonshak, A. (ed.). Spirulina platensis (Arthrospira): Physiology, Cell-biology and Biotechnology. Londres: ... "Administration of the hot-water extract of Spirulina platensis enhanced the immune response of white shrimp Litopenaeus ...

https://gl.m.wikipedia.org/wiki/Espirulina

"Plant and Cell Physiology. 43 (7): 718-725. doi:10.1093/pcp/pcf091. ISSN 1471-9053. PMID 12154134.. ... the properties of starches according to the mixture of these related substances and variable saccharification by malt extract.[ ... This causes a high sense of biocompatibility with cells and molecules within the body. Amylopectin is also able to biodegrade ... Bionanocomposites have been used in tissue engineering to replace, support, or regenerate the cells, organs, or parts of human ...

https://en.wikipedia.org/wiki/Amylopectin

... denominator end local function extract_number(parms, text, another, no_fraction) -- Return true, info if can extract a number ... Return text for a user-specified style for a table cell, or '' if none, -- given i = 1 (input style) or 2 (output style). local ... extract_number(parms, valstr, i , 1) if not success and valstr and i , 20 then -- check i to limit abuse local lhs, sep, rhs = ... extract_number(parms, parms[i], false, true) end if success then i = i + 1 in_unit_table.altitude = info.value end end local ...

https://simple.wikipedia.org/wiki/Module:Convert

"Cell. 167 (3): 633-642.e11. doi:10.1016/j.cell.2016.09.028. PMC 5484524. PMID 27768887.. ... Blood is then extracted from the immunized horse. The serum is separated and further purified and freeze-dried. It is ... "Cell. 167 (3): 633-642.e11. doi:10.1016/j.cell.2016.09.028. PMC 5484524. PMID 27768887.. ... The thymus, located in fatty tissue above the heart, is responsible for the generation of immune cells in the blood. The ...

https://en.m.wikipedia.org/wiki/Snakes

Extracted teeth. Cause[edit]. Several theories regarding the aetiology of hypodontia have been proposed in existing literature ... which is important in regulating cell fate, proliferation, differentiation and apoptosis.[42] Its polymorphic variant may be ...

https://en.wikipedia.org/wiki/Hypodontia

... while the corresponding cells of phoronids', brachiopods' and pterobranchs' lophophores have one cilium per cell; and bryozoan ... Chemicals extracted from a marine bryozoan species have been investigated for treatment of cancer and Alzheimer's disease, but ... Multiciliated cells in epithelium Yes[20]. no[20]. Yes[20]. not applicable ... A group of chemicals called bryostatins can be extracted from the marine bryozoan Bugula neritina. In 2001 pharmaceutical ...

https://en.wikipedia.org/wiki/Bryozoa

The rounded growths are filled with a spongy mass and a single wasp larva is located in a hard, seed-like cell in the centre.[7 ... Oak marble gall extract is used in deodorants because of tannic acid's anti-bacterial properties.[21] ... Sectioned gall showing central 'cell' and inquiline chamber; exit-hole and a possibly parasitised stunted gall specimen ... both bank voles and yellow-necked mice feed on larvae and pupae extracted from oak marble galls.[15] ...

https://en.m.wikipedia.org/wiki/Andricus_kollari

Cette raison a engagé M. Mosander à donner au nouveau métal le nom de Lantane." (The oxide of cerium, extracted from cerite by ... a b Kuipers, J., Giepmans, B.N.G. Neodymium as an alternative contrast for uranium in electron microscopy. Histochem Cell Biol ... J Cell Biol 4:475-478 *^ Hosogi N, Nishioka H, Nakakoshi M (2015) Evaluation of lanthanide salts as alternative stains to ... He X, Liu B (2017) Oolong tea extract as a substitute for uranyl acetate in staining of ultrathin sections based on examples of ...

https://en.wikipedia.org/wiki/Neodymium

Lossy formats are often used for the distribution of streaming audio or interactive communication (such as in cell phone ...

https://en.wikipedia.org/wiki/Audio_compression_

They extracted cells from young mouse embryos and injected them into another mouse of different strains. When the mouse ... cells gradually acquire the ability to distinguish between their own tissue substances on the one hand and unwanted cells and ... "Peter Brian Medawar". Cell. Immunol. 62 (2): 235-42. August 1981. doi:10.1016/0008-8749(81)90319-1. PMID 7026052.. ... Lawrence, H. (August 1981). "Advances in immunology: a meeting in honor of Sir Peter Medawar". Cell. Immunol. 62 (2): 233-310. ...

https://en.wikipedia.org/wiki/Peter_Medawar

ASIN: B00085KPWK) (online extract pages 155-157, 210-211). *. Lehmann, Ernst A.; Mingos, Howard. 1927. The Zeppelins. The ... separate multiple internal gas cells, each free to expand and contract thus obviating the need for ballonets; modular frame ... allowing addition of sections and gas cells; controls, engines and gondola rigidly attached. After publishing the idea in March ...

https://en.wikipedia.org/wiki/Ferdinand_von_Zeppelin

It recounted that police found photos and messages on Turner's cell phone that indicated extensive drug use, including LSD, ... ecstasy, marijuana extracts, and excessive alcohol. Turner was arrested in 2014 for possession of alcohol while under legal age ... However, evidence recovered from his cell phone texts recorded in the year before his 2015 arrest showed that he had ... her underwear and cell phone having been dropped beside her.[33] Jonsson and Arndt saw Turner thrust his hips into the woman,[ ...

https://en.m.wikipedia.org/wiki/People_v._Turner

... (/ˈkɔɪər/), also called coconut fibre, is a natural fibre extracted from the outer husk of coconut[1] and used in products ... The individual fibre cells are narrow and hollow, with thick walls made of cellulose. They are pale when immature, but later ... The coir fibre thus extracted is then combed using steel combs to make the fibre clean and to remove short fibres. Bristle coir ... become hardened and yellowed as a layer of lignin is deposited on their walls.[14] Each cell is about 1 mm (0.04 in) long and ...

https://en.wikipedia.org/wiki/Coir

Surprise discovery of bird-like blood cells in dinosaur bones suggests that fleshy remains might be commonplace even in poorly ... Although the cells are unlikely to contain DNA, those extracted from better preserved fossils using the same technique may do ... Blood cells came from this claw (Image: Laurent Mekul). "But the fossils we looked at were not rare at all," she says. ... Instead, they found blood-like cells and collagen from 75-million-year-old dinosaur fossils - 10 million years before T. rex ...

https://www.newscientist.com/article/dn27687-dinosaur-blood-cells-extracted-from-75-million-year-old-fossil/

Cell Extract Kit for studying Cell Extracts in the research area. ... Apoptosis Cell Extracts (Jurkat Untreated): Total cell extracts ... Apoptosis Cell Extracts (Jurkat + Etoposide): Total cell extracts from Jurkat cells treated with 25 μM etoposide for 5 hours ... Jurkat Apoptosis Cell Extracts (etoposide) 2043. Toggle Between Dark and Light Modes Filter: *WB ... Western blot analysis of Jurkat Apoptosis Cell Extracts #2043 using Caspase-3 (8G10) Rabbit mAb, #9665.. Show Less Show More ...

https://www.cellsignal.com/products/experimental-controls/jurkat-apoptosis-cell-extracts-etoposide/2043

We demonstrate that these in-house cell lysates provide sensor performance comparable to commercial PURE cell-free systems. We ... We then demonstrate that these in-house cell lysates provide sensor performance comparable to commercial PURE cell-free systems ... The local implementation of low-cost cell-free toehold sensors could enable biosensing capacity at the regional level and lead ... The local implementation of low-cost cell-free toehold sensors could enable biosensing capacity at the regional level and lead ...

https://www.frontiersin.org/articles/10.3389/fbioe.2021.727584/full

More from Plant Cell Extracts. Cutting edge research in plant science from The Plant Cell, published by the American Society of ... Reproduced from Movie S1 from Meena et al 2019, The plant Cell.. Like countries keeping an eye on their enemies, plant cells ... The same idea applies to cells. To import or export ions, plants have holes in their external cell wall. Moreover, like you can ... Reproduced from Figure 1E from Meena et al 2019, The Plant Cell.. Lets compare the plants in the figure above. The intact ...

https://medium.com/plant-cell-extracts/green-alert-how-to-wave-the-warning-flag-like-a-plant-b79367bdad6d

... peppers purported weight management properties after they revealed that piperine from black pepper extract may block fat cell ... finding that both black pepper extract and piperine strongly inhibited the fat cell differentiation of 3T3-L1 cell, without ... "We found that Piper nigrum Linne [back pepper] extract and its constituent piperine reduce lipid storage in 3T3-L1 cells and ... Black pepper extract could fight fat by blocking cell formation. By Nathan Gray ...

https://www.nutraingredients.com/Article/2012/05/09/Black-pepper-extract-could-fight-fat-by-blocking-cell-formation

Placenta mesenchymal stem cells are suggested to be a useful stem cell source in cell therapy.Apr 06, 2012. ... 9 Abstracts with Placenta Extract (or Stem Cells) Research. Filter by Study Type. Animal Study. ... 4 Pharmacological Actions Researched for Placenta Extract (or Stem Cells) Name. AC. CK. Focus. ... 5 Diseases Researched for Placenta Extract (or Stem Cells) Name. AC. CK. Focus. ...

https://greenmedinfo.com/substance/placenta-extract-or-stem-cells

Some cells remained isolated but with altered morphology (Figure 1F). RD, Hep-2C, and BGM cells injected with an extract ... Image of mammalian cell lines injected with extracts from healthy shrimp: A, human rhabdomyosarcoma (RD), Cep-2C, BGM RD cells ... Image of mammalian cell lines injected with extracts from healthy shrimp: A, human rhabdomyosarcoma (RD), Cep-2C, BGM RD cells ... The cells showed an apparent increase in size, diffuse cell rounding, and a refringent aspect (Figure 1B). In Hep-2C cells, the ...

https://wwwnc.cdc.gov/eid/article/9/2/02-0181_article

The inhibition of water-extracted branch of C. cassia (WBCC) on the activity of PDHK using both in vitro and cell-based kinase ... Water-extracted branch of Cinnamomum cassia promotes lung cancer cell apoptosis by inhibiting pyruvate dehydrogenase kinase ... WBCC reduced viabilities of several lung cancer cells with minimal cytotoxicity on normal bronchial epithelial cells. WBCC ... This is first report that WBCC induces apoptosis of lung cancer cells through inhibiting PDHK activity. Our findings suggest ...

https://pubmed.ncbi.nlm.nih.gov/30392804/

Uptill now, using this, I have not been able to extract values of cells which… ... this, I have not been able to extract values of cells which are. evaluated using formulae. Also, the excel files that I need to ... cell(col,row). returns the content of a cell. The upper left corner is (1,1) or (A,. 1) ... also need values of cells which have formulae, aslo some of the cells In. the excel refer to different excel files or different ...

https://www.ruby-forum.com/t/excel-parsing-extract-values-of-cells-with-formulae/117267

A question was raised within my digital forensic unit and I just wanted to hear additional inputIf you have a cell phone with ... Unless you are "extracting" logical file, in this case a video file, how would you distinguish one block of data from an other ... If you have a cell phone with multiple videos stored on the device and you only wanted to pull only one of them, I know ... If you have a cell phone with multiple videos stored on the device and you only wanted to pull only one of them, I know ...

https://www.forensicfocus.com/forums/mobile-forensics/extracting-video-from-cell-phone/

Most of the glucuronosyltransferase activity in the cell-free extract was found in a 20000 g particulate fraction. Optimum pH ... The primer ability of native cell walls was comparable to that of mucoric acid. ... Two different polyuronide fractions isolated from the cell walls of this fungus were used as primers. Mucoran, a heteropolymer ... Cell-free extracts from Mucor rouxii contain enzymes that catalyse the synthesis of uridine diphosphate glucuronic acid ( ...

https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-128-9-2023

Cell viability was determined using MTT assay.. Measurement of cell cycle. AGS cells were treated with ethyl alcohol and ... Cell Signal. 2012;24:1420-5 21. Dorstyn L, Akey CW, Kumar S. New insights into apoptosome structure and function. Cell Death ... Alisma canaliculatum Extract Affects AGS Gastric Cancer Cells by Inducing Apoptosis Min Ji Kwon1, Jeong Nam Kim1, Joon Park2,3 ... Alisma canaliculatum Extract Affects AGS Gastric Cancer Cells by Inducing Apoptosis. Int J Med Sci 2021; 18(10):2155-2161. doi: ...

https://medsci.org/v18p2155.htm

... cytoplasmic or whole-cell extract from mammalian cell or tissue samples with a detailed protocol to ensure your extract is not ... The Nuclear Extract Kit isolates high-quality nuclear, ... Cell, Acid, & Tissue Extracts * Cell Extracts. * Acid Extracts ... The Nuclear Extract Kit isolates high-quality nuclear, cytoplasmic or whole-cell extract from mammalian cell or tissue samples ... Active Motif also offers a number of ready-to-use nuclear, cytoplasmic and whole-cell extracts. These extracts have been ...

https://www.activemotif.com/catalog/100/nuclear-extract-kit

... which can extract the characters in the middle of the cell. In this example, what can we do to extract the four numbers 9135? ... If we want to extract the first three numbers from the left in cell B2, how can we realize it? Get into theFormulas tab, click ... Here we enter B2 into the input box, which means well extract characters from cell B2. The second parameter start_num stands ... The three practical functions can help extract characters and text content from cells. Make your work easier with WPS ...

https://www.wps.com/academy/use-the-text-function-to-extract-characters-in-cells/1861726/?chan=Mobile_campain

A fluid containing the contents of lysed cells is called a "lysate". Cell lysis is used to break open cells to avoid shear ... How do you extract protein from cell lysate?. January 4, 2021. by Rhyley Bryan ... 5. Using a cell scraper or silicone spatula, scrape the cells and transfer the lysate to a 15 ml conical. 6. Incubate the ... How do you extract protein from cell lysate?. Protein Extraction Protocol Steps ...

https://www.handlebar-online.com/other/how-do-you-extract-protein-from-cell-lysate/

With the ability to teach your skin to be young again, imagine a "chemical messenger" that can enter your skin cells and do ...

https://www.cellmedicine.com/news212/

... , a news source for Brock University ... The team exposed these cells to rosemary extract and studied the extracts impacts on a signalling pathway called Akt and on ... rosemary extract stops the growth of cancer cells.. At the core of the research is understanding cell signalling - a complex ... The team found that the rosemary extract:. *Stopped the survival and spread of cancer cells and enhanced the process of ...

https://brocku.ca/brock-news/2017/02/brock-research-finds-that-rosemary-extract-reduces-lung-cancer-cell-growth/

Cells were seeded in 48-well plates at a concentration of 2×104 cells/well and incubated for 24 h at 37°C to allow the cells to ... Exposure of the cells to tanshinones transformed the shapes of the cells from polygonal to circular, and resulted in cell ... Cytotoxic effect of extract fractions D and E on five human cancer cell lines (IC50, μg/ml). ... Cytotoxic effect of extract fractions D and E on five human cancer cell lines (IC50, μg/ml). ...

https://www.spandidos-publications.com/10.3892/etm.2015.2252

The Effects of Viscum album (Mistletoe) QuFrF Extract and Vincristine in Human Multiple Myeloma Cell Lines - A Comparative ...

https://www.intechopen.com/chapters/32143

... was powdered and extracted in refluxing n-hexane the extract was chromatographed on silica gel and eluted with gradient n- ... labeled secondary antibodies were purchased from Cell Signaling Technology (MA). Celastrol was extracted as previously reported ... donate to gastric tumor cell proliferation and migration [17] and work as an oncogene in human being colorectal tumor cells [18 ... Celastrol is an all natural substance extracted in the place triperygium. * Post author By exposed ...

http://www.exposed-skin-care.net/2016/03/01/celastrol-is-an-all-natural-substance-extracted-in-the-place-triperygium/

... a T cell-mediated disease with rising prevalence. Models of αβ T cell response emphasize T cell receptor (TCR) contact with ... a T cell-mediated disease with rising prevalence. Models of αβ T cell response emphasize T cell receptor (TCR) contact with ... a T cell-mediated disease with rising prevalence. Models of αβ T cell response emphasize T cell receptor (TCR) contact with ... a T cell-mediated disease with rising prevalence. Models of αβ T cell response emphasize T cell receptor (TCR) contact with ...

https://research.monash.edu/en/publications/human-t-cell-response-to-cd1a-and-contact-dermatitis-allergens-in

... chromatin condensation and loss of cell-cell contacts which were known as sign of apoptosis. In conclusion, green tea extract ... The Role of Green Tea Extract on the Proliferation of Human Ovarian Cancer Cells (in vitro) Study. International Journal of ... Abstract: This study investigated the role of green tea extracts as a cytotoxic agent against human ovarian cancer cells in ... Cytotoxic Effect of Berberis vulgaris Fruit Extract on the Proliferation of Human Liver Cancer Cell Line (HepG2) and its ...

https://scialert.net/abstract/index.php?doi=ijcr.2010.78.88

Here, we describe the effects of whole cranberry extract (WCE) on the behaviour of DU145 human prostate cancer cells in vitro. ... Recently, there has been interest in examining the effects of whole extracts from commonly harvested crops on the behaviour and ... proportion of cells in the G2-M phase of the cell cycle and increased the proportion of cells in the G1 phase of the cell cycle ... extract affects human prostate cancer cell growth via cell cycle arrest by modulating expression of cell cycle regulators ...

https://www.cranberryinstitute.org/cranberry-health-research/abstract/american-cranberry-vaccinium-macrocarpon-extract-affects-human-prostate-cancer-cell-growth-via-cell-cycle-arrest-by-modulating-expression-of-cell

Dissection of the Cell Cycle Using Cell-Free Extracts From Xenopus Laevis. Research output: Contribution to Journal/Magazine › ... Extracts for studying M phase can be prepared from unfertilized eggs or from interphase cells approaching mitosis, but always ... the use of these and derivative extracts has expanded prodigiously. The relative ease with which these extracts are made from ... However, free calcium in extracts is present at physiological levels. A calcium-mediated event is required, not only in exit ...

https://www.research.lancs.ac.uk/portal/en/publications/dissection-of-the-cell-cycle-using-cellfree-extracts-from-xenopus-laevis

Abstract #954: Guggulipid, the extract from the Commiphora mukul tree, induces apoptosis in human prostate cancer cells by ... Since JNK can be activated ROS, we subsequently explored the effect of GL on ROS generation in human PCa cells using oxidative ... In LNCaP and C-81 cells, GL treatment at low concentrations caused a dose-dependent increase in ROS that peaked around 2.5 ... The GL-induced ROS production in both PCa cell lines was transient and limited to the first 2 hours post-exposure. In ...

https://aacrjournals.org/cancerres/article/69/9_Supplement/954/553047/Abstract-954-Guggulipid-the-extract-from-the

About the Journal. As one of the first Open Access journals in its field, Food & Nutrition Research (FNR) offers an important forum for researchers to exchange the latest results from research on human nutrition broadly and food-related nutrition in particular. Learn more about the journals Aims & Scope. FNR is widely indexed by relevant services and databases, including PubMed Central/PubMed, Scopus, Science Citation Index, with an Impact Factor of 3.89 (2020).. ...

https://foodandnutritionresearch.net/index.php/fnr/article/view/1189/4477

Effect of Erica australis extract on Caco-2 cells, fibroblasts and selected pathogenic bacteria responsible for wound infection ...

https://www.ccmar.ualg.pt/en/node/4601

Cell cycle analysis on RSC96 Schwann cells showed that, after exposure to Alpinia oxyphylla Miq. fruit extract, the transition ... fruits in RSC96 Schwann cell proliferation. Our current results showed that treatment with the extract of Alpinia oxyphylla Miq ... fruits facilitate the survival and proliferation of RSC96 cells via insulin-like growth factor-1 signaling. ... which resulted in a reduction of the expression of the survival factor B-cell lymphoma 2. We provide positive results that ...

https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-017-1695-2

The extract "significantly induced death in breast cancer cells." She also says it stopped breast cancer cells from growing and ... Tropical Extract Kills 80 Percent of Breast Cancer Cells. In All Health Watch, Cancer, Featured Article ... Two Percent Concentration Kills Cancer Cells. The tropical extract comes from the fruit of the bitter melon. Like its name, the ... Ray applied a two percent bitter melon extract to breast cancer cells. She found that this was the minimum amount needed to ...

https://www.institutefornaturalhealing.com/2012/04/tropical-extract-kills-80-percent-of-breast-cancer-cells/

Thus, ensuring that they are safe for human consumption is a critical issue for the preparation of plant extracts as dietary supplements. (spandidos-publications.com)
Torricelumn™, plant stem cells, blue-green algae, and 3 unique forest plant extracts offer antioxidant and soothing properties help boost hydration, revealing plumper, more youthful-looking eyes. (elizabethgrant.com)
Jones then gathered all the plant extracts that fixed his issues into a digestible supplement. (williamsburgplace.com)

What is the mechanisms of action to inhibit cancer cell proliferation? (brocku.ca)
reported that overexpressing miR-223 decreased migration increased cell death in anoikis conditions and augmented sensitivity to chemotherapy but had no effect on adhesion and proliferation [15]. (exposed-skin-care.net)
In vitro proliferation assays were performed to compare the growth rate of all groups of cells. (scialert.net)
fruits in RSC96 Schwann cell proliferation. (biomedcentral.com)
fruits facilitate the survival and proliferation of RSC96 cells via insulin-like growth factor-1 signaling. (biomedcentral.com)
Schwann cells are capable of dedifferentiation, migration, proliferation, the expression of growth-promoting factors and the myelination of regenerating axons. (biomedcentral.com)
Therefore, enhancing Schwann cell proliferation might be a potential approach for neuron regeneration in neuron injury. (biomedcentral.com)
Cell Proliferation. (rupress.org)
From an immune perspective, tests have also reportedly shown a dose-dependent increase in interleukin-2 (IL-2), proteins made by white blood cells which plays a key role in T-cell activation, proliferation, and regulation. (nutraingredients-usa.com)
Cinnamon extract strongly inhibited tumor cell proliferation in vitro and induced active cell death of tumor cells by up-regulating pro-apoptotic molecules while inhibiting NFκB and AP1 activity and their target genes such as Bcl-2 , BcL-xL and survivin . (biomedcentral.com)
These agents inhibit cell growth and proliferation. (medscape.com)
Subsequently, this blocks DNA and RNA polymerases, resulting in the inhibition of cell growth and proliferation. (medscape.com)
Curcumol reduced the proliferation of breast cancer cells by targeting NCL/ERα36 and inactivating the PI3K/AKT pathway' - See curcumin at Amazon.com . (qualitycounts.com)
10 Astragalus polysaccharides are also shown to promote proliferation and function of intestinal intraepithelial T cells - a group of specialized T cells in the gastrointestinal mucosa that may also have systemic immune modulating effects. (ndnr.com)
In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
Building on maturation, and T cell proliferation would cover the critical the previous development of a time course in vitro human skin steps leading to induction. (cdc.gov)

Dihydrotanshinone I was observed to exhibit a higher cytotoxic potential compared with the other tanshinones in the majority of the examined cell lines. (spandidos-publications.com)
This study investigated the role of green tea extracts as a cytotoxic agent against human ovarian cancer cells in vitro using different concentrations of green tea extracts (20, 50,100 and 250 μg mL -1 ) for 24 h. (scialert.net)
The purpose of this study was to investigate the cytotoxic effects of CECF on U-2 OS human osteosarcoma cells. (umn.edu)
Regarding to Caco-2 cells, the coloured extract exhibited the most cytotoxic effects, while the total extract was the most efficient in protecting these cells against oxidative damage induced by t-BHP. (preprints.org)
Some of them have not yet reported its cytotoxic activity in those cell lines. (elsevier.com)
The aims of this study were to evaluate the wound healing potential in vivo and the cytotoxic effects in vitro of Psidium guajava (Myrtaceae) leaf extract and commonly used corticosteroids. (bvsalud.org)

Western blot analysis of Jurkat Apoptosis Cell Extracts #2043 using Caspase-7 Antibody, #9492. (cellsignal.com)
Western blot analysis of Jurkat Apoptosis Cell Extracts #2043 using PARP Antibody #9542 (left), and Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb #5625 (right). (cellsignal.com)
Western blot analysis of Jurkat Apoptosis Cell Extracts #2043 using Caspase-3 (8G10) Rabbit mAb, #9665. (cellsignal.com)
Load 10 µl of untreated and etoposide treated Jurkat Apoptosis Control Cell Extracts (etoposide) per lane. (cellsignal.com)
Apoptosis Cell Extracts (Jurkat Untreated): Total cell extracts from Jurkat cells serve as a negative control. (cellsignal.com)
Apoptosis Cell Extracts (Jurkat + Etoposide): Total cell extracts from Jurkat cells treated with 25 μM etoposide for 5 hours serve as a positive control for activated apoptotic cascades. (cellsignal.com)
Apoptosis is a regulated physiological process leading to cell death. (cellsignal.com)
This is first report that WBCC induces apoptosis of lung cancer cells through inhibiting PDHK activity. (nih.gov)
In every test using different concentrations, oligosaccharides induced programmed cell death ( apoptosis ) at greater levels than the chemo drug . (naturalsociety.com)
It is reported that apple polysaccharide can prevent colon cancer growth and impede colon cancer progression…The aim of this study is to explore the effect of apple oligosaccharide on the cellular viability of human colon carcinoma cells (HT29 cells) and its mechanism…These results indicated that apple oligosaccharide attenuated HT29 cell viability by inducing cell apoptosis and cell cycle arrest. (naturalsociety.com)
These results suggest that ACE induces apoptosis in AGS gastric cancer cells, and therefore, ACE may have the potential to treat gastric cancer. (medsci.org)
Apoptosis plays a vital role in maintaining the balance between cell death and cell division. (medsci.org)
Apoptosis induced abnormalities can result in uncontrolled cell division, leading to diseases such as cancer [ 12 ]. (medsci.org)
Thus, apoptosis has become a key tool in cancer treatment, and the mechanism underlying cancer cell growth has become an important target for cancer cell therapy [ 13 ]. (medsci.org)
Cancer cells also override a process called apoptosis, where a cell programs itself to die. (brocku.ca)
Morphologically, the images of the fixed and stained human ovarian cancer cells with Coomassie stain revealed that the Green tea extracts causing cells shrinkage, blabbing, chromatin condensation and loss of cell-cell contacts which were known as sign of apoptosis. (scialert.net)
In our earlier studies, GL (standardized to z-guggulsterone) inhibited cell viability in androgen-dependent (LNCaP) and androgen-independent (C-81, DU145) cultured human PCa cells, in part by selectively inducing apoptosis in a dose-dependent manner. (aacrjournals.org)
Finally, pretreatment with antioxidant N-acetylcysteine (NAC) conferred significant protection against GL-induced apoptosis in LNCaP and C-81 cells, confirming that ROS generation is required for a significant proportion of GL-induced apoptosis. (aacrjournals.org)
The lack of full protection with NAC also indicated the presence of alternative non-ROS mediated mechanisms by which GL induces apoptosis in human PCa cells. (aacrjournals.org)
In conclusion, GL induces apoptosis in human PCa cells by causing ROS generation independent of androgen-responsiveness. (aacrjournals.org)
Western blotting was used to examine apoptosis-associated protein levels in U-2 OS cells after exposed to CECF. (umn.edu)
CECF decreased the percentage of viability, induced DNA damage and DNA condensation, G 0 /G 1 arrest, and apoptosis in U-2 OS cells. (umn.edu)
These findings suggest that CECF triggers apoptosis in U-2 OS cells via ROS-modulated caspase-dependent and -independent pathways. (umn.edu)
hence selective killing of tumor cells by promoting apoptosis pathway is an attractive and effective way for development of anti-cancer agents. (biomedcentral.com)

The inhibition of water-extracted branch of C. cassia (WBCC) on the activity of PDHK using both in vitro and cell-based kinase assay were examined in several lung cancer cells. (nih.gov)
Here, we describe the effects of whole cranberry extract (WCE) on the behaviour of DU145 human prostate cancer cells in vitro. (cranberryinstitute.org)
These findings demonstrate that phytochemical extracts from the American cranberry (Vaccinium macrocarpon) can affect the behaviour of human prostate cancer cells in vitro and further support the potential health benefits associated with cranberries. (cranberryinstitute.org)
Because the study was conducted in vitro, further clinical studies are needed to understand how the dandelion extract is absorbed and utilized in biological systems of the human body. (consciouslifenews.com)
In this study, we tested anti-tumor activity and elucidated action mechanism of cinnamon extract using various types of tumor cell lines including lymphoma, melanoma, cervix cancer and colorectal cancer in vitro and in vivo mouse melanoma model. (biomedcentral.com)
Oral administration of cinnamon extract in melanoma transplantation model significantly inhibited tumor growth with the same mechanism of action observed in vitro . (biomedcentral.com)
Our study suggests that anti-tumor effect of cinnamon extracts is directly linked with enhanced pro-apoptotic activity and inhibition of NFκB and AP1 activities and their target genes in vitro and in vivo mouse melanoma model. (biomedcentral.com)
This study aimed to assess in vitro cytoprotective effects of Arrabidaea chica Verlot extract, at 1 and 5 µg mL-1, associated with a fraction enriched with geranylgeraniol from Bixa orellana L., at 1, 7, and 15 µg mL-1, in keratinocytes exposed to zoledronic acid (ZA). (academicjournals.org)
Although clinical trials are mostly lacking, Astragalus has been credited with immune-modulating actions via numerous molecular, cell culture, animal, and in-vitro research, including effects on T cells, T-cell receptors, and cytokines. (ndnr.com)
For the in vitro toxicity assay, each substance was applied to mucosa fibroblast cell cultures in conditioned media. (bvsalud.org)
In vitro, the guava extract caused a decrease in the cell viability and growth when compared to the control and corticosteroids. (bvsalud.org)
Although in a short-term cytotoxicity analysis, the guava extract reduced the cell population in vitro, while in vivo, the extract accelerated wound healing. (bvsalud.org)
Estimates of majority of the data considered were measures of reactivity, the uncertainty, as well as average values of the epidermal both purely chemical and in vitro cell based, whereas epidermal disposition kinetics parameters, were made by fitting to the time disposition was simply represented by the octanol-water course skin permeation data from individual skin donors. (cdc.gov)

Um and his colleagues investigated the underlying mechanisms of the fat-fighting activity of black pepper extract and its constituent piperine - finding that both black pepper extract and piperine strongly inhibited the fat cell differentiation of 3T3-L1 cell, without affecting cytotoxicity. (nutraingredients.com)
WBCC reduced viabilities of several lung cancer cells with minimal cytotoxicity on normal bronchial epithelial cells. (nih.gov)
This experiment concerns cytotoxicity in both cancer and normal cells of their methanol extracts from wood, bark and leaf parts. (elsevier.com)
The leaf extracts of M. pruinosa, M. tanarius, M. trichocarpa showed more cytotoxicity than wood and bark in all cancer cell lines. (elsevier.com)
Prior to the quantification of Aβ 1-40 peptide with an enzyme-linked immunosorbent assay (ELISA), we determined the cytotoxicity of the extracts using an MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, assay to discern whether decreases in Aβ 1-40 concentrations were the result of cell death. (emerginginvestigators.org)
Endothelial cell cytotoxicity of cotton bracts tannin and aqueous cotton bracts extract: tannin is the predominant cytotoxin present in aqueous cotton bracts extract. (cdc.gov)
MTT cytotoxicity assay demonstrates that our phyto-synthesized ZnFe 2 O 4 nanoparticles exhibited a selective and potent anticancer activity against K562 and MDA-MB-231 cell lines with IC50 values 4.53 μM and 4.19 μM, respectively. (nih.gov)

abstract = "Power conversion efficiencies of perovskite solar cells (PSCs) have rapidly increased from 3.8% to a certified 25.2% within only a decade. (edu.sa)

Like countries keeping an eye on their enemies, plant cells have 'sentinels', proteins that look out for troubles. (medium.com)
As shown in Figure 1, proteins that serve as markers for cellular fractionation, such as nuclear marker RNA pol II and cytoplasmic marker alpha Tubulin, were only detected in their respective cellular fractions and in whole-cell extracts. (activemotif.com)
Figure 1: Western blots of specific proteins from cellular fractions obtained with the Nuclear Extract Kit. (activemotif.com)
To evaluate the efficiency of cellular fractionation, 20 μg of nuclear (Nu), cytoplasmic (Cy) and whole-cell (WC) extracts, along with nuclear pellet collected during nuclear extraction using the Nuclear Extract Kit were analyzed by Western blot using antibodies against various specific proteins. (activemotif.com)
Cell lysis is used to break open cells to avoid shear forces that would denature or degrade sensitive proteins and DNA. (handlebar-online.com)
Usually, proteins are isolated from cells or tissues via lysis. (handlebar-online.com)
Lysis breaks down the cell membrane to separate proteins from the non-soluble parts of the cell. (handlebar-online.com)
As a result, extracted proteins become degraded or artifactually modified by the activities of these molecules. (handlebar-online.com)
Some cell lysis and protein solubilization methods cause the denaturation of proteins. (handlebar-online.com)
The team exposed these cells to rosemary extract and studied the extract's impacts on a signalling pathway called Akt and on certain proteins within the pathway. (brocku.ca)
These alterations in cell cycle were associated with changes in cell cycle regulatory proteins and other cell cycle associated proteins. (cranberryinstitute.org)
The expression of the cell cycle regulatory proteins cyclin D1, cyclin E and cyclin A increased in a dose-dependent manner. (biomedcentral.com)
Gel electrophoresis of gels shrunken by added myosin or electrophoresis of the proteins which can be sedimented from extract after incubation in the presence of HMM indicate that both myosin and HMM interfere with the changes in sedimentability of the high molecular weight protein (HMWP) thought to participate (together with actin) in gel formation in HeLa cell extracts (R. R. Weihing, 1976. (rupress.org)
A German university study found that the common dandelion ( Taraxacum officinale ) can block spike proteins from binding to the ACE2 cell surface receptors in human lung and kidney cells. (consciouslifenews.com)
Better yet, the dandelion extract could prove to prevent infections altogether, by blocking the precise channel by which the spike proteins attach and cause viral replication. (consciouslifenews.com)
Cytoskeleton offers several reagents for live-cell research including fluorescent proteins, cell permeable protein activators and inhibitors, as well as our recent addition of live cell imaging probes. (cytoskeleton.com)

The 21 methanol extracts were prepared from seven Macaranga tree species (Macaranga bancana, Macaranga gigantea, Macaranga hullettii, Macaranga pruinosa, Macaranga tanarius, Macaranga trichocarpa and Macaranga triloba). (elsevier.com)
It was found that the ethyl acetate extracts from barks (VR12874) and fruits (VR11626) of G. hanburyi exhibited strong growth inhibitory activity against five CCA cell lines with IC50 values range from 1.84±0.10 to 2.49±0.03 mg/ml and 1.69±0.04 to 4.41±0.10 mg/ml, respectively whereas the methanol extracts from barks (VR12875) and fruits (VR11627) showed no growth inhibitory activity on CCA cell lines tested. (who.int)
It was found that five fractions including VR12877, VR12878, VR12881, VR12882 and VR12883 showed strong growth inhibitory activity whereas VR12876 and VR12879 exhibited low growth inhibitory activity and VR12880 showed no effect.Discussion and conclusion: It could be concluded that the ethyl acetate extracts from barks and fruits of G. hanburyi showed strong growth inhibitory effect against 5 human CCA cell lines whereas the methanol extracts had no effect. (who.int)

In recent research , compounds in apples known as oligosaccharides were found to kill up to 46% of human colon cancer cells . (naturalsociety.com)
The lipid-soluble compounds, usually extracted using alcohol solvents, are rich in abietanoids and diterpene quinones (tanshinones). (spandidos-publications.com)
Screening of structurally related compounds revealed additional stimulants of CD1a-restricted T cells, including farnesol and coenzyme Q2. (monash.edu)
The researchers used high molecular weight compounds taken from a water-based dandelion extract and put them to the test in human HEK293-hACE2 kidney and A549-hACE2-TMPRSS2 lung cells. (consciouslifenews.com)
These natural compounds (along with dandelion extract) can be readily mass-produced, combined, and deployed as preventative medicine for all future spike protein variants. (consciouslifenews.com)
There were seven major terpenoid compounds in the leaf extracts, including 2 monoterpenes of camphor and L-borneol, and 5 sesquiterpenes of silphiperfol-5-ene, 7-epi-silphiperfol-5-ene, ß-caryophyllene, ɤ-eudesmol, and α-eudesmol. (biorxiv.org)
Fuse, H 1995, ' Transformation of Dimethyl Sulfide and Related Compounds by Cultures and Cell Extracts of Marine Phyto plankton ', Bioscience,Biotechnology, and Biochemistry , vol. 59, pp. 1773-1775. (elsevier.com)
Antioxidants are compounds that help neutralize harmful molecules called free radicals, preventing cell damage ( 1 ). (healthline.com)
Live-cell imaging probes developed by Spirochrome are cell-permeable compounds which stain microtubules (SiR-Tubulin), F-actin (SiR-Actin), Lysosomes (SiR-Lysosome) and chromosomal DNA (SiR-DNA) in living cells. (cytoskeleton.com)
We carry fan favorites like aloe vera and colloidal oatmeal, as well as more exotic compounds like marine seaweed extract and carrot cells. (makingcosmetics.com)

Dried S. miltiorrhiza root was extracted with various solvents and under varying extraction conditions, and the effects of the extracts on the viability of five human cancer cell lines were compared. (spandidos-publications.com)
Finally we attempted to get the feasible molecular basis where miR-223 modifications affected mobile viability in cells treated or not treated with celastrol. (exposed-skin-care.net)
Following treatment of DU145 human prostate cancer cells with 10, 25 and 50 mg ml 1 of WCE, respectively for 6 h, WCE significantly decreased the cellular viability of DU145 cells. (cranberryinstitute.org)
Cells were submitted to assessments of MTT cell viability, antiproliferative activity, and clonogenic assay potential. (academicjournals.org)
With an association of A. chica at 1 and 5 µg mL-1 and a fraction enriched with geranylgeraniol at 15 µg mL-1, an increase was observed in cell viability of 73.5% and 71.1%, respectively. (academicjournals.org)
The antiproliferative activity showed this treatment was not able to increase the viability of tumor cells, while the clonogenic assay potential assessment showed the association with A. chica at the highest concentration tested (5 µg mL-1) was able to reverse the effects of ZA on the cells. (academicjournals.org)
Cell viability. (academicjournals.org)

Myosin mixed with extract induces shrinkage of the gel, but jelled extract or myosin alone does not shrink. (rupress.org)

Aqueous extract of human placenta, used as wound healer, has shown significant cell adhesion property on mouse peritoneal macrophages and P388D1 cultured macrophage cell line. (elsevier.com)
Nath, S & Bhattacharyya, D 2007, ' Cell adhesion by aqueous extract of human placenta used as wound healer ', Indian Journal of Experimental Biology , vol. 45, no. 8, pp. 732-738. (elsevier.com)
In this study, we investigated the effect of an aqueous extract from A. millefolium on experimental autoimmune encephalomyelitis (EAE) and on the serum cytokine levels in C57BL/6 mice. (ijp-online.com)
Following immunization, the treatment protocol was initiated by using different doses of an aqueous extract from A. millefolium (1, 5, and 10 mg/mouse/day). (ijp-online.com)
The results demonstrated that treatment with aqueous extract of A. millefolium may attenuate disease severity, inflammatory responses, and demyelinating lesions in EAE-induced mice. (ijp-online.com)
In the present study, zinc-iron bimetallic nanoparticles (ZnFe 2 O 4 ) were synthesized via an aqueous extract of Boswellia carteri resin mixed with zinc acetate and iron chloride precursors, and they were tested for their anticancer activity. (nih.gov)

The Nuclear Extract Kit isolates high-quality nuclear, cytoplasmic or whole-cell extract from mammalian cell or tissue samples. (activemotif.com)

These sensors, however, have been mainly implemented using reconstituted PURE cell-free protein expression systems that are difficult to source in the Global South due to their high commercial cost and cold-chain shipping requirements. (frontiersin.org)
The Nuclear Extract Kit works with fresh or frozen cell and tissue samples and yields protein amounts of approximately 0.5 to 1 mg from cytoplasmic extract (at 1-2 mg/ml concentration), 150 to 250 μg from nuclear extract (at 3-5 mg/ml concentration), 1.2 to 2.4 mg from whole cell extract (at 4-8 mg/ml concentration) from 8.8 x 10 6 cells. (activemotif.com)
Extracts prepared using the Nuclear Extract Kit can be used with Active Motif's TransAM ® Kits , or in other procedures that require cell extracts, like gelshift assays, Western blots, DNA footprinting, as a starting point for protein purification for binding or activity assays or or other analysis techniques. (activemotif.com)
How do you extract protein from cell lysate? (handlebar-online.com)
How do you get protein samples from cells? (handlebar-online.com)
How does cell lysate protein extraction take place? (handlebar-online.com)
The amount of lysis buffer should be empirically determined for each cell type to ensure efficient lysis as well as an optimal final concentration of protein in the lysate. (handlebar-online.com)
Transfection with a small interfering RNA blocked the expression of phosphatidylinositol 3-kinase and induced down-regulation both on the mRNA and protein levels, which resulted in a reduction of the expression of the survival factor B-cell lymphoma 2. (biomedcentral.com)
The water-based dandelion extract, taken from the plant's dried leaves, was effective against spike protein D614 and a host of mutant strains, including D614G, N501Y, K417N, and E484K. (consciouslifenews.com)
The dandelion blocked the protein-to-protein interactions between the S1 sub unit of the spike protein and the human ACE2 cell surface receptor. (consciouslifenews.com)
Hydroalcoholic pomegranate peel extract blocks the spike protein at the ACE2 receptor with 74 percent efficacy. (consciouslifenews.com)
Fig. 2: SarkoSpin extracts from different disease subtypes have distinct protein compositions beyond TDP-43. (nature.com)
A MiniblotterTM device (Immunetics, Cambridge, MA) is used to immobilize antigenic extracts and protein-A standards on nitrocellulose membranes (Amersham 2020D). (cdc.gov)
Dexamethasone increases airway epithelial cell neutral endopeptidase by enhancing transcription and new protein synthesis. (cdc.gov)
The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). (cellsignal.com)
Sickle-cell disease is a genetic condition in which the red blood cells contain an abnormal form of the oxygen-carrying protein haemoglobin S. Children who inherit sickle-cell genes from both parents will develop sickle-cell disease, while those who inherit the gene from only one parent will have the sickle-cell trait. (who.int)
But the researchers noted a trend in the women on the extract toward lowered cholesterol and decreased vascular endothelial growth factor, a protein which, in elevated levels, may signify the beginning of cancerous spread. (prevention.com)
Specifically, information about epidermal disposition, step in human skin sensitization induction is the transport of sensitizer from the applied dose on the skin surface to the protein reactivity, epidermal inflammation, dendritic cell epidermis, where innate immune activation occurs. (cdc.gov)

These studies identify molecular connections between CD1a and hypersensitivity to consumer products, defining a mechanism that could plausibly explain the many known T cell responses to oily substances. (monash.edu)
A special extract active substances horsetail increases the life cycle of hair. (rv.ua)
Preparations of cell constituents or subcellular materials, isolates, or substances. (bvsalud.org)

On the anti-inflammatory potential, data indicates the extract can suppress numerous pro-inflammatory cytokines in a dose-dependent manner in cell models, with additional data showing activity against the inflammatory response to UV stress in skin cells. (nutraingredients-usa.com)
Due to the herbal extracts' common anti-inflammatory property, the experiment determined whether or not this property had the potential to change Aβ 1-40 concentrations. (emerginginvestigators.org)
In this study, we found that coffee extract at more than 2.5%(v/v) significantly inhibited LPS-induced inflammatory responses in RAW264.7 cells and that anti-inflammatory activity of coffee required the roasting process. (nature.com)
an overnight lotion with 2.5 percent Benzoyl Peroxide, and an oil-free, glycerin-based moisturizer with physical and chemical sunscreen and cinnamon extract, a natural anti-inflammatory and anti-bacterial. (beautynewsnyc.com)

These herbs are generally recognized as safe, and there are no known cases of overdose with dandelion leaf extract . (consciouslifenews.com)
Effect of aqueous leaf extract of Azadirachta indica on the reproductive organs in male mice. (contraceptionjournal.org)
Reversible antifertility effect of aqueous leaf extract of Allamanda cathartica L. in male laboratory mice. (contraceptionjournal.org)

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. (cellsignal.com)
Anti-phospho-HSF-1 (Ser326) anti-phospho-mTOR (Ser2481) and anti-mTOR were purchased from Epitomics (CA). Anti-β-action anti-HSP70 anti-phospho-NF-κB (Ser536) and horseradish peroxidase (HRP)-labeled secondary antibodies were purchased from Cell Signaling Technology (MA). (exposed-skin-care.net)
SignalSilence ® mTOR siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit mTOR expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. (cellsignal.com)

This study aimed to compare three different extracts of Saco sweet cherry, namely non-coloured fraction, coloured fraction and total extract concerning phenolic composition, antioxidant and antidiabetic potential, erythrocytes' protection and effects on Caco-2 cells. (preprints.org)
Volatiles and antioxidant capacities in essential oils (EOs) from fresh immature and mature leaves of Blumea balsamifera, extracted with different extraction periods of hydrodistillation, were investigated. (biorxiv.org)
In fact, one test-tube study found that treating cells with goji berry extract significantly increased antioxidant levels and reduced several markers of oxidative stress ( 7 ). (healthline.com)
4 University of Madrid researchers called Spirulina a "powerful antioxidant" that was capable of interfering with cell death caused by free radical damage. (bodyecology.com)
The researchers suggested that Spirulina extract could be useful in some treatments for neurodegenerative disorders - since Spirulina helped to increase levels of the body's most important antioxidant, glutathione. (bodyecology.com)

Overexpression of MECP2 attenuates cigarette smoke extracts induced lung epithelial cell injury by promoting CYP1B1 methylation. (cdc.gov)

NFκB activation was assayed with the TransAM ® NFκB p50 Kit using increasing amounts of nuclear and cytoplasmic extracts isolated from unstimulated and stimulated HeLa cells using the Nuclear Extract Kit. (activemotif.com)

To demonstrate the efficiency of cellular fractionation using the Nuclear Extract Kit, nuclear, cytoplasmic, and whole-cell extracts were prepared from HeLa cell samples. (activemotif.com)
To demonstrate the quality of the materials and protocol of the Nuclear Extract Kit, nuclear and cytoplasmic extracts were prepared from HeLa cell samples that were either unstimulated or stimulated with TNF-α for 30 minutes. (activemotif.com)
Effects of myosin and heavy meromyosin on actin-related gelation of HeLa cell extracts. (rupress.org)
The gelation induced by warming (to 25 degrees C) the 100,000 g supernatant fraction (extract) of HeLa cells lysed in a buffer containing sucrose, ATP, DTE, EGTA, imidazole, and Triton X-100 was studied in the presence of myosin and heavy meromyosin (HMM). (rupress.org)
These results, together with previous results showing that actin is present and that HMWP is enriched in the plasma membrane fraction of HeLa cells (R. R. Weihing, 1976. (rupress.org)
Western blot analysis of extracts from HeLa cells, transfected with either control siRNA (-) or SignalSilence ® mTOR siRNA I (+), using mTOR Antibody #2972 and eIF4B Antibody #3592. (cellsignal.com)
Fluorescent detection of SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency. (cellsignal.com)

Water soluble cinnamon extract was obtained and quality of cinnamon extract was evaluated by HPLC (High Performance Liquid Chromatography) analysis. (biomedcentral.com)

HMM (0.4-0.8 mg/ml) can inhibit gel formation by extract in tubes or floated on a sucrose cushion. (rupress.org)

fruits triggers the phosphorylated insulin-like growth factor-1 receptor- phosphatidylinositol 3-kinase/serine-threonine kinase pathway, and up-regulated the proliferating cell nuclear antigen in a dose-dependent manner. (biomedcentral.com)
One attractive strategy is the discovery of new drugs from medicinal plants which have a potential growth inhibitory activity on cholangiocarcinoma cells.Objective: To examine the growth inhibitory activity of crude extracts and fractions from barks and fruits of Garcinia hanburyi on human cholangiocarcinoma cell lines.Method: Growth inhibitory activity was assessed by sulforhodamine B assay. (who.int)
The mean IC50 values of three independent experiments were reported.Results: The growth inhibitory activity of four extracts from barks and fruits of G. hanburyi on five human CCA cell lines was performed. (who.int)
Thanks to their growing popularity, goji berries are also frequently featured in supplements and superfood blends alongside other fruits, herbs, and extracts. (healthline.com)

The extract "significantly induced death in breast cancer cells. (institutefornaturalhealing.com)
Conclusion: Watermelon seeds extract significantly increases the number of fibroblasts immunoreactive cells expressing iNOS after 72 hours on Guinea pig. (researchgate.net)
A treatment with 5%(v/v) coffee extract and more than 2.5 μM pyrocatechol inhibited the LPS-induced activation of NF-κB and also significantly activated Nrf2, which acts as a negative regulator in LPS-induced inflammation. (nature.com)

Herbal extracts and dietary supplements may be extracted from the medicinal plants used in traditional Chinese medicine, and are used increasingly commonly worldwide for their benefits to health and quality of life. (spandidos-publications.com)
Therefore, treating Schwann cells with Chinese herbal medicines to enhance their therapeutic potential in guiding neuron regrowth is considered a possible approach to treat nerve injury. (biomedcentral.com)
The objective of the experiment was to test the effects of various herbal extracts (bugleweed, hops, sassafras, and white camphor) on Aβ 1-40 peptide levels in human neuroblastoma cells that were transfected to overexpress APP. (emerginginvestigators.org)
These natural vitiligo remedies include vitamins, amino acids, and herbal extracts. (progressivehealth.com)

Two different polyuronide fractions isolated from the cell walls of this fungus were used as primers. (microbiologyresearch.org)
Subsequent to these results eight fractions from VR12874 were examined for their growth inhibitory activities against 5 human CCA cell lines. (who.int)

In addition, WBCC induced ROS- and mitochondria-dependent apoptotic cell death. (nih.gov)

Organic botanicals, extracted with the latest CO2 technology, are incorporated into our Alphasomes® skin care delivery system to assure effectiveness and purity without chemical additives. (ca-botana.com)

The total extract was the most effective against DPPH, nitric oxide and superoxide radicals, and in the inhibition of α-glucosidase enzyme. (preprints.org)

The number of poly-morpho-nuclear, fibroblasts, endothelial and macrophage cells expressing iNOS are counted in all groups using light microscopes. (researchgate.net)
0.05) on the number of immunoreactive fibroblasts cells on experimental group 2 compared to control group 2. (researchgate.net)
Various analytic methods were applied for the characterization of the phyto synthesized ZnFe 2 O 4 , and they were tested for their anticancer activity against MDA-MB-231, K562, MCF-7 cancer cell lines, and normal fibroblasts. (nih.gov)
Ultrastructurally, most studies have concluded that the foam cells in verruciform xanthoma are fat-laden macrophages, although other cell types, including Langerhans cells and even fibroblasts, have been proposed. (medscape.com)
[ 11 , 92 ] Cobb et al described myelin figures and reduplication of the overlying basal lamina and fibroblasts in addition to macrophagelike cells, which contained lipid inclusions. (medscape.com)

When analyzed by coal type, organic solvent extracts of bituminous and subbituminous coal produced the most SCEs, followed by lignite and peat. (cdc.gov)
Organic anthracite extracts did not induce SCEs. (cdc.gov)
Organic extracts of all five coal types induced cell cycle delays. (cdc.gov)
Organic solvent extracts of bituminous and subbituminous coal dust were tested with lymphocytes from five other donors to assess variability in the responses. (cdc.gov)
Keravita Pro is comprised of a combination of organic natural plant and vitamin extracts. (williamsburgplace.com)
This method uses a mixture of organic solvents, phenol and chloroform, to extract unwanted cellular components from nucleic acid. (cdc.gov)
Add the organic mixture consisting of trizol and chloroform directly to a cell lysate and mix thoroughly to generate a biphasic emulsion. (cdc.gov)

The team also produced a review paper "Anticancer Effects of Rosemary (Rosmarinus officinalis L.) Extract and Rosemary Extract Polyphenols" published in the November 2016 issue of the journal Nutrients . (brocku.ca)

Placenta-derived stem cells exhibit neuroprotective properties in an animal model of stroke. (greenmedinfo.com)
Placenta mesenchymal stem cells are suggested to be a useful stem cell source in cell therapy. (greenmedinfo.com)
Placenta-derived mesenchymal stem cells of maternal origin displays plasticity indicating their potential value in disease treatment. (greenmedinfo.com)
Stem Cells Int. 2012 ;2012:174328. (greenmedinfo.com)
Placenta-derived stem cells have anti-proliferative properties against cancer cell lines. (greenmedinfo.com)
Activation of endogenous stem cell mobilization can contribute to myocardial regeneration after ischemic injury. (biomedcentral.com)
This study aimed to evaluate the possible role of Avemar or Echinacea extracts in inducing mobilization and homing of CD34 + stem cells in relation to the inflammatory and hematopoietic cytokines in rats suffering from acute myocardial infarction (AMI). (biomedcentral.com)
Avemar and Echinacea extracts can effectively induce mobilization and homing of CD34 + stem cells to the myocardial tissue and thus may help in stem cell-based regeneration of the infarcted myocardium. (biomedcentral.com)
In last decade, stem cell (SC) therapy has emerged as a potential new strategy for incurable and life-threating MI. (biomedcentral.com)
Stem cells and gene therapy may be used in the future. (medlineplus.gov)
Researchers Dr. Magdelena Pasarica and Dr. Nikhil Dhurandhar collected fatty tissue from 10 patients having liposuction from which they extracted stem cells. (news-medical.net)
they found over a period of one week that many of the stem cells exposed to the virus had been converted into early stage fat cells and begun storing fat. (news-medical.net)
In samples that were not infected, only a third as many stem cells had become fat cells. (news-medical.net)
Hair raising Japanese researchers have successfully grown hair on hairless mice by implanting follicles created from stem cells, sparking new hopes of a cure for baldness. (abc.net.au)
When stem cells are grown into tissues or organs, they usually need to be extracted from embryos, but Tsuji and his researchers found hair follicles can be grown with adult stem cells. (abc.net.au)
Our current study thus demonstrates the potential for not only hair regeneration therapy but also the realisation of bioengineered organ replacement using adult somatic stem cells," the researchers write. (abc.net.au)
Powerful Peptides, natural antioxidants, and Grape Stem Cells give your skin a needed boost to improve radiance and clarity for a brighter, flawless complexion. (ca-botana.com)
PICO 1: What is the age limit for autologous stem cell tion? (bvsalud.org)
question, "What is the age limit for autologous stem cell transplantation in patients with Multiple Myeloma? (bvsalud.org)

Mice of the Parkes (P) strain were orally administered aqueous rhizome extract of C. longa (600 mg/kg body weight per day for 56 and 84 days), and effect of the treatment on various male reproductive endpoints and fertility was evaluated. (contraceptionjournal.org)

Finally, the oligosaccharides were added at various concentrations to cultured human HT29 colon cancer cells, while a common chemo drug was added to others. (naturalsociety.com)
At about 0.9 PPM), oligosaccharides killed 17.6% of the colon cancer cells within 36 hours. (naturalsociety.com)
At 9.0 PPM, 46% of colon cancer cells were killed by oligosaccharides. (naturalsociety.com)
The anti-cancer effects of Alisma canaliculatum extracts (ACE) were identified in AGS gastric cancer cells. (medsci.org)
However, the anti-cancer roles of A. canaliculatum on gastric cancer cells have not yet been studied. (medsci.org)
Thus, we investigated the mechanisms underlying the anti-cancer effects of A. canaliculatum extract (ACE) on AGS gastric cancer cells. (medsci.org)
We have some evidence that rosemary extract stops the growth of cancer cells," Tsiani said. (brocku.ca)
Tsiani, her graduate student Jessy Moore and colleagues from the Departments of Health Sciences, Kinesiology and the Centre for Bone and Muscle Health, set out to see if, and how, rosemary extract stops the growth of cancer cells. (brocku.ca)
Cancer cells are created when there are errors in the way a cell processes information during a signalling pathway, often through mutations in one or more molecules. (brocku.ca)
The research team grew non-small lung cancer cells in its lab. (brocku.ca)
It has been reported that miR-223 influences the survival ability of various cancer cells [13]. (exposed-skin-care.net)
found that miR-223 promoted the invasion of breast cancer cells via CCL2 the Mef2c-β-catenin pathway [14] while Pinatel et al. (exposed-skin-care.net)
The results of statistical analysis revealed significant decrease in the number of treated human ovarian cancer cells inversely proportion with increasing the concentration of green tea extracts, this experiments determined that the IC 50 = 100 μg mL -1 . (scialert.net)
She also says it stopped breast cancer cells from growing and spreading. (institutefornaturalhealing.com)
In her study, Dr. Ray applied a two percent bitter melon extract to breast cancer cells. (institutefornaturalhealing.com)
She found that this was the minimum amount needed to kill a significant number of breast cancer cells. (institutefornaturalhealing.com)
In fact, at that concentration, the extract killed 80 percent of breast cancer cells. (institutefornaturalhealing.com)
Resveratrol reverses TGF-β1-mediated invasion and metastasis of breast cancer cells via the SIRT3/AMPK/autophagy signal axis - Phytother Res 2022 Sep 9 - 'Taken together, our study provided novel insight into the anticancer effects of Resv and revealed that targeting the SIRT3/AMPK/autophagy pathway can serve as a new therapeutic target against breast cancer' - See resveratrol products at Amazon.com . (qualitycounts.com)

"Our findings suggest that piperine, a major component of black pepper, inhibits fat cell differentiation by down-regulating the transcriptional activity of PPARγ (and LXRα) and suppressing the expression of PPARγ (and LXRα)," said the researchers, led by Soo-Jong Um from Sejong University, Korea. (nutraingredients.com)
They added that the findings may lead to the wider use of piperine or black-pepper extracts in fighting obesity and related diseases. (nutraingredients.com)
These findings indicated the extracts from leaves of M. pruinosa, M. tanarius and M. trichocarpa had more potential than wood and bark parts to be developed as anti-cancer agents. (elsevier.com)
Gene expression analysis of distinct populations of cells isolated from mouse and human inner ear FFPE tissue using laser capture microdissection - a Technical report based on preliminary findings. (cdc.gov)
New findings out of Columbia University's Medical Center suggest that when women with breast cancer take oral green tea extract, Polyphenon E, their conditions improve. (prevention.com)

The present study investigated extracts of Salvia miltiorrhiza Bunge (S. miltiorrhiza), traditionally used in Asian countries to treat a variety of conditions, as a dietary supplement or as an ingredient in functional foods. (spandidos-publications.com)

These results indicated that the ethyl acetate extracts might contain active constituents. (who.int)

And even without DNA, soft tissue cells and molecules could help us learn much more about dinosaur physiology and behaviour, the team says. (newscientist.com)
Microscopic image of the subcuticular tissue of the pleopod from a shrimp infected with the supernatant of the third passage of a human larynx carcinoma (Hep-2C) cell culture inoculated with an. (cdc.gov)
At the core of the research is understanding cell signalling - a complex communications system that regulates basic activities and co-ordinates actions within a cell, resulting in such processes as normal cell development and growth and tissue repair. (brocku.ca)
Such increases in circulating CD34 + cells were accompanied by increased homing to myocardial tissue 14 days after AMI. (biomedcentral.com)
Basically, the efficacy of SC therapy in regenerative medicine depends on sufficient recruitment of available cells (either exogenously administered populations or endogenously mobilized residents) to the target tissue. (biomedcentral.com)
The scientists from Pennington Biomedical Centre at Louisiana State University, conducted laboratory tests on human fatty tissue and found that the virus triggered changes in the tissue that left people with more, and larger, fat cells than people who were not infected. (news-medical.net)
Laser Capture Microdissection (LCM) allows microscopic procurement of specific cell types from tissue sections that can then be used for gene expression analysis. (cdc.gov)
For example, following exposure to noise , ototoxic drugs or age, it would be highly desirable to analyze gene expression profiles of selected populations of cells within the organ of Corti or spiral ganglion cells rather than a mixed population of cells from whole inner ear tissue. (cdc.gov)
This photomicrograph revealed histopathology exhibited by a tissue sample extracted from an established lesion, diagnosed as granuloma inguinale, also known as Donovanosis. (cdc.gov)
This tissue sample was extracted from the patient depicted in PHIL 6440. (cdc.gov)
The most striking and characteristic histologic feature of the verruciform xanthoma is the presence of large foam cells in the connective-tissue papillae. (medscape.com)
In vivo, guava extract caused accelerated wound healing from the 3rd day, whereas the corticosteroids delayed tissue repair and were associated with bacterial surface colonization, the presence of micro-abscesses and intense inflammatory infiltrate in the submucosa. (bvsalud.org)

Figure 2: Specific extraction of nuclear and cytoplasmic extracts. (activemotif.com)
The RIGHT function starts to extract characters from the right to the left, while the LEFT function starts the extraction from the left to the right. (wps.com)
Akonni Biosystems, a life science tools/molecular diagnostics company, will present results of its work to develop an automated extraction method to isolate cell-free fetal DNA from maternal plasma, using its TruTip ® extraction products, at the ASHG conference in San Francisco on November 8. (labwrench.com)
Traditionally, water or vegetable oil is used for this purpose, although solvent extraction is also employed to produce annatto extracts with an higher pigment content. (inchem.org)
Gently remove dead skin cells and target the pores for a fast and easy extraction. (exit15.com)
We failed to extract Brucella DNA with a commercial kit, but an extraction kit designed in-house using 2 sets of primers [B4/B5 (223 bp) and JPF/JPR (193 bp)] was successful and more economical. (who.int)
The platform utilizes an algorithms for segmentation, feature extraction and classifications that were based on online cervical cell datasets. (who.int)

Celastrol continues to be discovered to induce temperature surprise response in multiple tumor cell lines due to its activation of temperature surprise element-1 (HSF-1) [10]. (exposed-skin-care.net)

In conclusion, green tea extract has been shown antiproliferative activity against the growth of HOCC which might be considered in clinical situation. (scialert.net)
In conclusion, our biosynthesized ZnFe 2 O 4 nanoparticles show a promising, environmentally friendly, and low coast chemotherapeutic approach against selective cancers with a predicted low adverse side effect toward normal cells. (nih.gov)

Since JNK can be activated ROS, we subsequently explored the effect of GL on ROS generation in human PCa cells using oxidative stress indicator dyes H2DCFDA and HE. (aacrjournals.org)
Finally, the protective effect of the extracts to prevent oxidative damage in human erythrocytes was assessed. (preprints.org)
"Our story is really where science meets luck and the magic of biology and nature," said Voigt: DeBolt's initial discovery was fortunate, but having continued to research and develop the extracts, the company now understands that these bioactives work on three fundamental and interconnected physiologies - oxidative stress, inflammation, and immunity. (nutraingredients-usa.com)

The dandelion extract stopped SARS-CoV-2 spike pseudotyped lentivirus particles from attaching to lung cells and stopped an inflammatory process called interleukin-6 secretion. (consciouslifenews.com)
Furthermore, intake of 60% (v/v) coffee extract and 74.4 μM pyrocatechol, which is the concentration equal to contained in 60% (v/v) coffee, markedly inhibited the LPS-induced inflammatory responses in mice. (nature.com)
EAE-induced mice, which were treated with A. millefolium , had less cerebral infiltration of inflammatory cells. (ijp-online.com)

Studies of mutagenicity, additional long-term (1year) studies in rats, and observations of the effects of annatto extract in humans were also considered. (inchem.org)
Evidence in humans and animal models suggests that IgE-mediated mast cell activation gives rise to both the acute and late-phase responses. (jci.org)

In this study, we further identified that anti-tumor effect of cinnamon extracts is also link with enhanced pro-apoptotic activity by inhibiting the activities NFκB and AP1 in mouse melanoma model. (biomedcentral.com)

Extracts obtained using 100% ethanol and 100% acetone as solvents exhibited more potent effects compared with extracts obtained using 70 and 30% aqueous ethanol. (spandidos-publications.com)
Celastrol was extracted as previously reported by us [30 31 briefly the air-dried root bark of triperygium wilfordii Hook F (from Fujian Province China) was powdered and extracted in refluxing n-hexane the extract was chromatographed on silica gel and eluted with gradient n-hexane/acetone. (exposed-skin-care.net)
Peripheral lymphocytes from three healthy nonsmoking donors were incubated with dichloromethane plus acetone or aqueous extracts of anthracite, bituminous, subbituminous coal, lignite, or peat dust or snuff alone or in combination. (cdc.gov)

Models of αβ T cell response emphasize T cell receptor (TCR) contact with peptide-MHC complexes, but this model cannot readily explain activation by most contact dermatitis allergens, which are nonpeptidic molecules. (monash.edu)
This property was offered primarily by fibronectin type III like peptide present in the extract and is comparable to fibronectin on a molar basis. (elsevier.com)
The peptide induce adhesion of cell through cell surface receptors having K d = 2.8±0.9 × 10 -5 M suggesting weak binding. (elsevier.com)
Cell adhesion was partially inhibited by Arg-Gly-Asp (RGD) peptide, anti-β 1 integrin suggesting that integrin β 1 receptors have roles to play in the process. (elsevier.com)

Using CD1a-autoreactive human αβ T cell clones to screen clinically important allergens present in skin patch testing kits, we identified responses to balsam of Peru, a tree oil widely used in cosmetics and toothpaste. (monash.edu)
Neuron injury stimulates various physiological responses that facilitate nerve cell regeneration. (biomedcentral.com)
Th2 cells are critical in maintaining both the state of chronic and relapsing eosinophil-predominant inflammation and the acute hypersensitivity responses characteristic of the atopic diseases. (jci.org)
IgE-induced mast cell degranulation in vivo is often followed by a late-phase reaction (LPR), a second wave of hypersensitivity responses occurring many hours after the acute reaction and dependent upon eosinophils. (jci.org)
Acute responses are accompanied by evidence of mast cell activation and mediator release. (jci.org)

1990. Cell replication and unscheduled DNA synthesis (UDS) activity of low molecular weight chlorinated paraffins in the rat liver in vivo. (cdc.gov)

For example, narcotic drugs like opioids attach to certain receptors in brain cells. (medlineplus.gov)

The extract is also suggested to have anti-cancer properties, and could enhance cognitive functions and provide anti-depressant effects. (nutraingredients.com)
RD, Hep-2C, and BGM cells injected with an extract similarly processed but from healthy shrimp, showed no cytopathic effects, even after 7 days of culture ( Figures 1A , 1C , and 1 E ). As a positive control, RD cells were injected with Sabin viral extract and showed the characteristic cytopathic effect produced by an enterovirus infection. (cdc.gov)
It exhibits various pharmacological properties, such as anti-bacterial and anti-cancer effects, and protects the liver cells [ 6 - 8 ]. (medsci.org)
The next step in the research, she said, is to "find the chemicals or components of rosemary extract that are responsible for these anti-cancer effects. (brocku.ca)
Recently, there has been interest in examining the effects of whole extracts from commonly harvested crops on the behaviour and progression of cancer. (cranberryinstitute.org)
The effects on cell cycling were determined. (cdc.gov)
Resveratrol (Res) exerts inhibitory effects on breast cancer cell lines and animal models, while its efficacy against individual breast cancer cases remains unknown. (qualitycounts.com)
Astragaloside IV is the primary pure saponin of Astragalus , and has been shown to be important to its cytokine and T-cell modulation effects, promoting T-helper cells toward a T-helper 1 (Th1) phenotype. (ndnr.com)

Rats pre- and post-treated with Avemar or Echinacea exhibited substantial increases in the number of circulating CD34 + cells, peaking on the first day after AMI to approximately 13-fold and 15-fold, respectively, with a decline in their level on day 7 followed by a significant increase on day 14 compared to their corresponding AMI levels. (biomedcentral.com)

The cytopathic effect in RD cells began with a partial destruction of the cellular layer. (cdc.gov)
The cytopathic effect in the BGM cell line began as a partial destruction of the cellular layer, which evolved to a syncytial-like formation of rounded, refringent cells. (cdc.gov)
Apple oligosaccharides don't negatively effect healthy cells, unlike chemo. (naturalsociety.com)
The effect of green tea extracts was also studied for long period (24, 48 and 72 h) using the IC 50 = 100 μg mL -1 . (scialert.net)
Previously, we have reported that anti-cancer effect of cinnamon extracts is associated with modulation of angiogenesis and effector function of CD8 + T cells. (biomedcentral.com)
However, there is no research on the effect of watermelon seed extract on the expression of i-NOS. (researchgate.net)
Purpose: The aim of this study is to prove the effect of watermelon seed extract on inducing the expression of i-NOS by immunoreactive cells. (researchgate.net)
The aim of the study was to assess the synergistic effect of green tea extract and curcumin in patients with oral potentially malignant disorders (OPMDs) and to ascertain the mechanism of action of these chemopreventive agents through assessment of suitable biomarkers. (kanker-actueel.nl)

One team studies the biology of sensations such as pain, and the other has expertise in cell imaging. (medlineplus.gov)
Dr. Yamada, who is Chief of the NIDCR Cell Biology Section, has expertise in developmental biology. (medlineplus.gov)

Hydroxycinnamic acids were the most predominant in both non-coloured fraction and total extract, while cyanidin-3-O-rutinoside was the main anthocyanin found in the coloured fraction. (preprints.org)

Amniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis. (greenmedinfo.com)

Characterization of procyanidin extracts from hawthorn (Crataegus pinnatifida) in human colorectal adenocarcinoma cell line Caco-2, simulated Digestion, and fermentation identified unique and novel prebiotic properties. (bvsalud.org)

We demonstrate that these in-house cell lysates provide sensor performance comparable to commercial PURE cell-free systems. (frontiersin.org)
Here we demonstrate that TSV also infects human cell lines, which may suggest that Penaeus is a potential reservoir of this virus. (cdc.gov)

In LNCaP and C-81 cells, GL treatment at low concentrations caused a dose-dependent increase in ROS that peaked around 2.5 microM. (aacrjournals.org)
No evidence was found for the accumulation of annatto pigments in the tissues of rats fed at low concentrations (20 220 mg/kg bw per day of annatto extracts containing up to 2.3% bixin/norbixin mixture) for 1 year. (inchem.org)

Serially diluted (1/500, 1/1000 and 1/2000) serum from each subject as well as human IgG standards (2000 ng/ml, 1000 ng/ml, 500 ng/ml and 250 ng/ml) are loaded perpendicularly to the bacterial extracts, and are allowed to interact. (cdc.gov)
In this case the invention resided in the observation that cell free foetal DNA (cffDNA) is to be found in maternal plasma (or serum), coupled with the explanation of how to extract the cffDNA from the plasma or serum. (ficpi.org)
The American College of Obstetricians and Gynecologists (ACOG) has recommended that serum screening, cell-free DNA screening, and diagnostic tests, such as chorionic villus sampling (CVS) and amniocentesis , be discussed with and offered to all women early in pregnancy, regardless of their age. (medscape.com)

Plasma glucose is determined using blood drawn into a gray-top (sodium fluoride) tube, which inhibits red blood cell glycolysis immediately. (medscape.com)

The freeze-thaw method is commonly used to lyse bacterial and mammalian cells. (handlebar-online.com)
Numerous diterpenoid tanshinones have been extracted from S. miltiorrhiza , including dihydrotanshinone I, cryptotanshinone and tanshinones I and IIA, which are the three most commonly studied. (spandidos-publications.com)
If larger crystals are formed in the process of growing crystals (usually 6 commonly oriented), and from such a crystal block are cut the plates to produce a solar cell, then such cells are called polycrystalline or multi-crystalline cells. (differencebetween.net)

The methods used to generate these extracts are considered first, together with the features that contribute to nuclear construction. (lancs.ac.uk)

Crude extract of Corni Fructus (CECF) has been used in Traditional Chinese medicine for the treatment of different diseases for hundreds of years. (umn.edu)

Subjects with OPMDs ( n = 60) were randomized to receive green tea extract [topical + systemic (800 mg/day)] or curcumin [topical + Systemic (950 mg/day)] or a combination therapy with 20 patients in each group for 3 months. (kanker-actueel.nl)
Treatment of OPMDs with curcumin and green tea extract combination demonstrated a significant clinical response supported by downregulation of molecular biomarkers in the short-term (12 weeks). (kanker-actueel.nl)
Scientists found that when mice consumed green tea extract, their endurance increased by up to 24% . (prevention.com)

Celastrol is an all natural substance extracted in the place triperygium wilfordii Hook F which includes been found in anti-inflammation and anti-cancer remedies in Chinese language folk medicine for quite some time. (exposed-skin-care.net)

In the ten years since Lohka and Masui developed extracts from amphibian eggs capable of nuclear assembly, DNA replication, and mitotic chromosome condensation, the use of these and derivative extracts has expanded prodigiously. (lancs.ac.uk)
In the present study, we found that resveratrol dramatically suppressed the replication of vaccinia virus (VACV), the prototypic member of poxviruses, in various cell types. (researchgate.net)

However, free calcium in extracts is present at physiological levels. (lancs.ac.uk)

Impaired translesion synthesis in xeroderma pigmentosum variant extracts. (1/1728)

Mutations of oncoprotein 18/stathmin identify tubulin-directed regulatory activities distinct from tubulin association. (2/1728)

Regulation of human hsp90alpha gene expression. (3/1728)

unr, a cellular cytoplasmic RNA-binding protein with five cold-shock domains, is required for internal initiation of translation of human rhinovirus RNA. (4/1728)

Fertilization, embryonic development, and offspring from mouse eggs injected with round spermatids combined with Ca2+ oscillation-inducing sperm factor. (5/1728)

Discrete regional distributions suggest diverse functional roles of calcium channel alpha1 subunits in sperm. (6/1728)

Defective repair of cisplatin-induced DNA damage caused by reduced XPA protein in testicular germ cell tumours. (7/1728)

Bcl-2 regulates a caspase-3/caspase-2 apoptotic cascade in cytosolic extracts. (8/1728)

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