Fatigue Syndrome, Chronic: A syndrome characterized by persistent or recurrent fatigue, diffuse musculoskeletal pain, sleep disturbances, and subjective cognitive impairment of 6 months duration or longer. Symptoms are not caused by ongoing exertion; are not relieved by rest; and result in a substantial reduction of previous levels of occupational, educational, social, or personal activities. Minor alterations of immune, neuroendocrine, and autonomic function may be associated with this syndrome. There is also considerable overlap between this condition and FIBROMYALGIA. (From Semin Neurol 1998;18(2):237-42; Ann Intern Med 1994 Dec 15;121(12): 953-9)Pleurodynia, Epidemic: An acute, febrile, infectious disease generally occurring in epidemics. It is usually caused by coxsackieviruses B and sometimes by coxsackieviruses A; echoviruses; or other enteroviruses.Bipolar Disorder: A major affective disorder marked by severe mood swings (manic or major depressive episodes) and a tendency to remission and recurrence.Attention Deficit Disorder with Hyperactivity: A behavior disorder originating in childhood in which the essential features are signs of developmentally inappropriate inattention, impulsivity, and hyperactivity. Although most individuals have symptoms of both inattention and hyperactivity-impulsivity, one or the other pattern may be predominant. The disorder is more frequent in males than females. Onset is in childhood. Symptoms often attenuate during late adolescence although a minority experience the full complement of symptoms into mid-adulthood. (From DSM-V)Amyotrophic Lateral Sclerosis: A degenerative disorder affecting upper MOTOR NEURONS in the brain and lower motor neurons in the brain stem and SPINAL CORD. Disease onset is usually after the age of 50 and the process is usually fatal within 3 to 6 years. Clinical manifestations include progressive weakness, atrophy, FASCICULATION, hyperreflexia, DYSARTHRIA, dysphagia, and eventual paralysis of respiratory function. Pathologic features include the replacement of motor neurons with fibrous ASTROCYTES and atrophy of anterior SPINAL NERVE ROOTS and corticospinal tracts. (From Adams et al., Principles of Neurology, 6th ed, pp1089-94)Gastroesophageal Reflux: Retrograde flow of gastric juice (GASTRIC ACID) and/or duodenal contents (BILE ACIDS; PANCREATIC JUICE) into the distal ESOPHAGUS, commonly due to incompetence of the LOWER ESOPHAGEAL SPHINCTER.Irritable Bowel Syndrome: A disorder with chronic or recurrent colonic symptoms without a clearcut etiology. This condition is characterized by chronic or recurrent ABDOMINAL PAIN, bloating, MUCUS in FECES, and an erratic disturbance of DEFECATION.Encephalomyelitis: A general term indicating inflammation of the BRAIN and SPINAL CORD, often used to indicate an infectious process, but also applicable to a variety of autoimmune and toxic-metabolic conditions. There is significant overlap regarding the usage of this term and ENCEPHALITIS in the literature.Anxiety Disorders: Persistent and disabling ANXIETY.Caliciviridae Infections: Virus diseases caused by CALICIVIRIDAE. They include HEPATITIS E; VESICULAR EXANTHEMA OF SWINE; acute respiratory infections in felines, rabbit hemorrhagic disease, and some cases of gastroenteritis in humans.Rejuvenation: The phenomenon of youthfulness, vitality, and freshness being restored. This can apply to appearance, TISSUES, organ functions, or other areas.Plastocyanin: A copper-containing plant protein that is a fundamental link in the electron transport chain of green plants during the photosynthetic conversion of light energy by photophosphorylation into the potential energy of chemical bonds.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Hematopoietic Stem Cells: Progenitor cells from which all blood cells derive.Stem Cell Transplantation: The transfer of STEM CELLS from one individual to another within the same species (TRANSPLANTATION, HOMOLOGOUS) or between species (XENOTRANSPLANTATION), or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). The source and location of the stem cells determines their potency or pluripotency to differentiate into various cell types.Embryonic Stem Cells: Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.Adult Stem Cells: Cells with high proliferative and self renewal capacities derived from adults.Skin Aging: The process of aging due to changes in the structure and elasticity of the skin over time. It may be a part of physiological aging or it may be due to the effects of ultraviolet radiation, usually through exposure to sunlight.Cosmetic Techniques: Procedures for the improvement or enhancement of the appearance of the visible parts of the body.Pluripotent Stem Cells: Cells that can give rise to cells of the three different GERM LAYERS.Telefacsimile: A telecommunication system combining the transmission of a document scanned at a transmitter, its reconstruction at a receiving station, and its duplication there by a copier.Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.JapanGreen Fluorescent Proteins: Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.Cellular Phone: Analog or digital communications device in which the user has a wireless connection from a telephone to a nearby transmitter. It is termed cellular because the service area is divided into multiple "cells." As the user moves from one cell area to another, the call is transferred to the local transmitter.Luminescent Proteins: Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.Cell-Free System: A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)Agriculture: The science, art or practice of cultivating soil, producing crops, and raising livestock.Fluorescence: The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.Faculty: The teaching staff and members of the administrative staff having academic rank in an educational institution.Dielectric Spectroscopy: A technique of measuring the dielectric properties of materials, which vary over a range of frequencies depending on the physical properties of the material. The technique involves measuring, over a range of frequencies, ELECTRICAL IMPEDANCE and phase shift of an electric field as it passes through the material.Solar Energy: Energy transmitted from the sun in the form of electromagnetic radiation.Recombination, Genetic: Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.Electric Impedance: The resistance to the flow of either alternating or direct electrical current.Gastrointestinal Transit: Passage of food (sometimes in the form of a test meal) through the gastrointestinal tract as measured in minutes or hours. The rate of passage through the intestine is an indicator of small bowel function.Chemical EngineeringPlethysmography, Impedance: Recording changes in electrical impedance between electrodes placed on opposite sides of a part of the body, as a measure of volume changes in the path of the current. (Stedman, 25th ed)Semiconductors: Materials that have a limited and usually variable electrical conductivity. They are particularly useful for the production of solid-state electronic devices.Crossing Over, Genetic: The reciprocal exchange of segments at corresponding positions along pairs of homologous CHROMOSOMES by symmetrical breakage and crosswise rejoining forming cross-over sites (HOLLIDAY JUNCTIONS) that are resolved during CHROMOSOME SEGREGATION. Crossing-over typically occurs during MEIOSIS but it may also occur in the absence of meiosis, for example, with bacterial chromosomes, organelle chromosomes, or somatic cell nuclear chromosomes.Cardiography, Impedance: A type of impedance plethysmography in which bioelectrical impedance is measured between electrodes positioned around the neck and around the lower thorax. It is used principally to calculate stroke volume and cardiac volume, but it is also related to myocardial contractility, thoracic fluid content, and circulation to the extremities.Invertebrate Hormones: Hormones produced by invertebrates, usually insects, mollusks, annelids, and helminths.Aplysia: An opisthobranch mollusk of the order Anaspidea. It is used frequently in studies of nervous system development because of its large identifiable neurons. Aplysiatoxin and its derivatives are not biosynthesized by Aplysia, but acquired by ingestion of Lyngbya (seaweed) species.Habituation, Psychophysiologic: The disappearance of responsiveness to a repeated stimulation. It does not include drug habituation.Reflex: An involuntary movement or exercise of function in a part, excited in response to a stimulus applied to the periphery and transmitted to the brain or spinal cord.Neurosecretion: The production and release of substances such as NEUROTRANSMITTERS or HORMONES from nerve cells.Learning: Relatively permanent change in behavior that is the result of past experience or practice. The concept includes the acquisition of knowledge.Oviposition: The process of laying or shedding fully developed eggs (OVA) from the female body. The term is usually used for certain INSECTS or FISHES with an organ called ovipositor where eggs are stored or deposited before expulsion from the body.Behavior, Animal: The observable response an animal makes to any situation.Neurons, Afferent: Neurons which conduct NERVE IMPULSES to the CENTRAL NERVOUS SYSTEM.Ganglia: Clusters of multipolar neurons surrounded by a capsule of loosely organized CONNECTIVE TISSUE located outside the CENTRAL NERVOUS SYSTEM.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.beta-Glucans: Glucose polymers consisting of a backbone of beta(1->3)-linked beta-D-glucopyranosyl units with beta(1->6) linked side chains of various lengths. They are a major component of the CELL WALL of organisms and of soluble DIETARY FIBER.Saccharomyces cerevisiae Proteins: Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.Glucans: Polysaccharides composed of repeating glucose units. They can consist of branched or unbranched chains in any linkages.Fungal Proteins: Proteins found in any species of fungus.Genes, Fungal: The functional hereditary units of FUNGI.Gene Expression Regulation, Fungal: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Phosphotransferases (Paired Acceptors): A group of enzymes that transfers two phosphate groups from a donor such as ATP to two different acceptors. (From Enzyme Nomenclature, 1992) EC 2.7.9.Saccharomyces: A genus of ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES.ColoradoPneumonia, Pneumococcal: A febrile disease caused by STREPTOCOCCUS PNEUMONIAE.Evolution, Planetary: Creation and development of bodies within solar systems, includes study of early planetary geology.Immunity, Innate: The capacity of a normal organism to remain unaffected by microorganisms and their toxins. It results from the presence of naturally occurring ANTI-INFECTIVE AGENTS, constitutional factors such as BODY TEMPERATURE and immediate acting immune cells such as NATURAL KILLER CELLS.Evans Blue: An azo dye used in blood volume and cardiac output measurement by the dye dilution method. It is very soluble, strongly bound to plasma albumin, and disappears very slowly.Pneumonia: Infection of the lung often accompanied by inflammation.Streptococcus pneumoniae: A gram-positive organism found in the upper respiratory tract, inflammatory exudates, and various body fluids of normal and/or diseased humans and, rarely, domestic animals.Colorado tick fever virus: A species of COLTIVIRUS transmitted by the tick DERMACENTOR andersonii and causing fever, chills, aching head and limbs, and often vomiting. It occurs in the northwestern United States, except the Pacific Coast.Lung: Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.Colorado Tick Fever: A febrile illness characterized by chills, aches, vomiting, leukopenia, and sometimes encephalitis. It is caused by the COLORADO TICK FEVER VIRUS, a reovirus transmitted by the tick Dermacentor andersoni.
Impaired translesion synthesis in xeroderma pigmentosum variant extracts. (1/1728)Xeroderma pigmentosum variant (XPV) cells are characterized by a cellular defect in the ability to synthesize intact daughter DNA strands on damaged templates. Molecular mechanisms that facilitate replication fork progression on damaged DNA in normal cells are not well defined. In this study, we used single-stranded plasmid molecules containing a single N-2-acetylaminofluorene (AAF) adduct to analyze translesion synthesis (TLS) catalyzed by extracts of either normal or XPV primary skin fibroblasts. In one of the substrates, the single AAF adduct was located at the 3' end of a run of three guanines that was previously shown to induce deletion of one G by a slippage mechanism. Primer extension reactions performed by normal cellular extracts from four different individuals produced the same distinct pattern of TLS, with over 80% of the products resulting from the elongation of a slipped intermediate and the remaining 20% resulting from a nonslipped intermediate. In contrast, with cellular extracts from five different XPV patients, the TLS reaction was strongly reduced, yielding only low amounts of TLS via the nonslipped intermediate. With our second substrate, in which the AAF adduct was located at the first G in the run, thus preventing slippage from occurring, we confirmed that normal extracts were able to perform TLS 10-fold more efficiently than XPV extracts. These data demonstrate unequivocally that the defect in XPV cells resides in translesion synthesis independently of the slippage process. (+info)
Mutations of oncoprotein 18/stathmin identify tubulin-directed regulatory activities distinct from tubulin association. (2/1728)Oncoprotein 18/stathmin (Op18) is a recently identified phosphorylation-responsive regulator of the microtubule (MT) system. It was originally proposed that Op18 specifically regulates dynamic properties of MTs by associating with tubulin, but it has subsequently been proposed that Op18 acts simply by sequestering of tubulin heterodimers. We have dissected the mechanistic action of Op18 by generation of two distinct classes of mutants. One class has interruptions of the heptad repeats of a potential coiled-coil region of Op18, and the other involves substitution at all four phosphorylation sites with negatively charged Glu residues. Both types of mutation result in Op18 proteins with a limited decrease in tubulin complex formation. However, the MT-destabilizing activities of the coiled-coil mutants are more severely reduced in transfected leukemia cells than those of the Glu-substituted Op18 derivative, providing evidence for tubulin-directed regulatory activities distinct from tubulin complex formation. Analysis of Op18-mediated regulation of tubulin GTPase activity and taxol-promoted tubulin polymerization showed that while wild-type and Glu-substituted Op18 derivatives are active, the coiled-coil mutants are essentially inactive. This suggests that Op18-tubulin contact involves structural motifs that deliver a signal of regulatory importance to the MT system. (+info)
Regulation of human hsp90alpha gene expression. (3/1728)Mammalian HSP90alpha and HSP90beta are encoded by two individual genes. On the basis of the upstream sequences of the human hsp90alpha gene, GenBank accession number U25822, we have constructed CAT reporter plasmids driven by individual fragments of the hsp90alpha gene. We found that (1) the proximal heat shock element complex located at -96/-60 enhances hsp90alpha promoter expression; (2) heat shock induction depends upon the coexistence of distal heat shock element at -1031/-1022 and the proximal heat shock element complex of the hsp90alpha gene; (3) unlike hsp90beta, downstream sequences of the transcription start site inhibit hsp90alpha expression. We conclude that the regulatory mechanisms for the expression of hsp90alpha and hsp90beta genes are different. (+info)
unr, a cellular cytoplasmic RNA-binding protein with five cold-shock domains, is required for internal initiation of translation of human rhinovirus RNA. (4/1728)Initiation of translation of the animal picornavirus RNAs occurs via a mechanism of direct ribosome entry, which requires a segment of the 5' UTR of the RNA, known as the internal ribosome entry site (IRES). In addition, translation of the enterovirus and rhinovirus (HRV) subgroups requires cellular trans-acting factors that are absent from, or limiting in rabbit reticulocytes, but are more abundant in HeLa cell extracts. It has been shown previously that HeLa cells contain two separable activities, each of which independently stimulates HRV IRES-dependent translation when used to supplement reticulocyte lysate; one of these activities was identified as polypyrimidine tract-binding protein (PTB). Here, the purification of the second activity is achieved by use of an RNA-affinity column based on the HRV 5' UTR. It comprises two components: a 38-kD protein (p38), which is a novel member of the GH-WD repeat protein family and has no intrinsic RNA-binding activity; and a 96- to 97-kD protein doublet, which was identified as unr, an RNA-binding protein with five cold-shock domains. Coimmunoprecipitation with antibodies against either protein shows that the two proteins interact with each other, and thus p38 is named unrip (unr-interacting protein). Recombinant unr acts synergistically with recombinant PTB to stimulate translation dependent on the rhinovirus IRES. In contrast, unr did not significantly augment the PTB-dependent stimulation of poliovirus IRES activity. (+info)
Fertilization, embryonic development, and offspring from mouse eggs injected with round spermatids combined with Ca2+ oscillation-inducing sperm factor. (5/1728)Round spermatids, precursor male gametes, are known to possess the potential to achieve fertilization and embryonic development when injected into eggs. However, injection of spermatids alone seldom activates eggs in the mouse, as spermatids by themselves cannot induce an increase in intracellular Ca2+, a prerequisite for egg activation. We injected a mouse round spermatid into an egg simultaneously with partially purified sperm factor from differentiated hamster spermatozoa. The combined injection produced repetitive Ca2+ increases (Ca2+ oscillations) lasting for at least 4 h as observed at fertilization, and induced activation in 92% of eggs. This method provided 75% fertilization success associated with male and female pronucleus formation and development to 2-cell embryos, while only 7% of eggs were fertilized by injection of a spermatid alone. Of the 2-cell embryos, approximately 50% developed to blastocysts during 5 days of culture in vitro, while no blastocysts were obtained following injection of sperm factor alone. Furthermore, the 2-cell embryos, that were created by spermatids and sperm factor and transplanted into foster mothers, developed into normal offspring, although the percentage was only 22%. All infants grew into healthy adults carrying normal chromosomes. The sperm factor served as a complementary factor for successful fertilization by round spermatid injection. (+info)
Discrete regional distributions suggest diverse functional roles of calcium channel alpha1 subunits in sperm. (6/1728)The Ca channels of male germ-line cells are partially characterized, but the molecular properties and subcellular localization of the Ca channels of mature sperm are unknown. Here, we probe rodent sperm with anti-peptide antibodies directed to cytosolic domains of cloned rat brain alpha1A, alpha1C, and alpha1E Ca channel subunits. Each recognizes a 200- to 245-kDa band on immunoblots of whole rat sperm extracts. A smaller ( approximately 110-kDa) alpha1C band also is detected. Confocal fluorescence images of mouse sperm show characteristic patterns of punctate alpha1A-, alpha1C-, and alpha1E-immunoreactivity. For alpha1A, the puncta are larger, less numerous, and more variable in distribution than for alpha1C and alpha1E. They are absent from the acrosomal crescent, but are present elsewhere over the sperm head, often at the apical tip and equatorial segment. They also are found at irregular intervals along both the midpiece and the principal piece of the flagellum. For alpha1C and alpha1E, puncta are dense along dorsal and ventral aspects of the acrosomal cap. For alpha1E but not alpha1C, the remainder of the acrosomal region also is labeled. Neither is found in the postacrosomal region or on the midpiece. Puncta of alpha1C and alpha1E occur at regular intervals each in two parallel rows, at the dorsal and ventral aspects of the proximal segment of the flagellar principal piece. The puncta in these arrays become less abundant and intense in the distal flagellum. These results demonstrate that multiple Ca channel proteins are present in mature sperm and are regionally localized in ways that may give them different regulatory roles. (+info)
Defective repair of cisplatin-induced DNA damage caused by reduced XPA protein in testicular germ cell tumours. (7/1728)Metastatic cancer in adults usually has a fatal outcome. In contrast, advanced testicular germ cell tumours are cured in over 80% of patients using cisplatin-based combination chemotherapy . An understanding of why these cells are sensitive to chemotherapeutic drugs is likely to have implications for the treatment of other types of cancer. Earlier measurements indicate that testis tumour cells are hypersensitive to cisplatin and have a low capacity to remove cisplatin-induced DNA damage from the genome  . We have investigated the nucleotide excision repair (NER) capacity of extracts from the well-defined 833K and GCT27 human testis tumour cell lines. Both had a reduced ability to carry out the incision steps of NER in comparison with extracts from known repair-proficient cells. Immunoblotting revealed that the testis tumour cells had normal amounts of most NER proteins, but low levels of the xeroderma pigmentosum group A protein (XPA) and the ERCC1-XPF endonuclease complex. Addition of XPA specifically conferred full NER capacity on the testis tumour extracts. These results show that a low XPA level in the testis tumour cell lines is sufficient to explain their poor ability to remove cisplatin adducts from DNA and might be a major reason for the high cisplatin sensitivity of testis tumours. Targeted inhibition of XPA could sensitise other types of cells and tumours to cisplatin and broaden the usefulness of this chemotherapeutic agent. (+info)
Bcl-2 regulates a caspase-3/caspase-2 apoptotic cascade in cytosolic extracts. (8/1728)Apoptosis is accompanied by the activation of a number of apoptotic proteases (caspases) which selectively cleave specific cellular substrates. Caspases themselves are zymogens which are activated by proteolysis. It is widely believed that 'initiator' caspases are recruited to and activated within apoptotic signalling complexes, and then cleave and activate downstream 'effector' caspases. While activation of the effector caspase, caspase-3, has indeed been observed as distal to activation of several different initiator caspases, evidence for a further downstream proteolytic cascade is limited. In particular, there is little evidence that cellular levels of caspase-3 that are activated via one pathway are sufficient to cleave and activate other initiator caspases. To address this issue, the ability of caspase-3, activated upon addition to cytosolic extracts of cytochrome c, to cause cleavage of caspase-2 was investigated. It was demonstrated that cleavage of caspase-2 follows, and is dependent upon, activation of caspase-3. Moreover, the activation of both caspases was inhibited by Bcl-2. Together, these data indicate that Bcl-2 can protect cells from apoptosis by acting at a point downstream from release of mitochondrial cytochrome c, thereby preventing a caspase-3 dependent proteolytic cascade. (+info)
"Methenyl-tetrahydromethanopterin cyclohydrolase in cell extracts of Methanobacterium". Arch. Biochem. Biophys. 242 (2): 430-9. ...
The antigens are either from cell extracts or recombinant. Blood serum is incubated in the wells of the plate and is washed out ... T-cells and B-cells) and antigen presenting cells. These cells coordinate an immune response upon the detection of foreign ... the cell line was contaminated and displaced by HeLa cells, and has now been identified as actually HeLa cells. They are ... They are termed ENA because they can be extracted from the cell nucleus with saline. The ENAs consist of ribonucleoproteins and ...
doi:10.1007/s13752-013-0155-6. Barbieri, M (1979). "Ribosome crystallization in homogenates and cell extracts of chick embryos ... and the Max Planck Institute of Molecular Cell Biology and Genetics in Berlin. Since 1992 he is professor of embryology at the ... in the cytoskeleton and in cell compartments. This adaptor-dependent definition of code has been used by Kühn and Hofmeyr to ...
In 1961, Eddy showed that an extract of rhesus monkey kidney cells (RMKC), notable because of its use as a growth medium in the ... Eddy BE, Borman GS, Berkely WH, Young RD (May 1961). "Tumors induced in hamsters by injection of rhesus monkey cell extracts". ... Eddy suggested that this contamination could be avoided in the future by screening cultures of C. aethiops kidney cells for the ... Eddy BE, Borman GS, Grubbs GE, Young RD (May 1962). "Identification of the oncogenic substance in rhesus monkey kidney cell ...
AYERS WA (1959). "Phosphorolysis and synthesis of cellobiose by cell extracts from Ruminococcus flavefaciens". J. Biol. Chem. ...
"Evidence for the presence of kinetin in DNA and cell extracts". FEBS Letters. 393 (2-3): 197-200. doi:10.1016/0014-5793(96) ... Callus cultures are not cell cultures, since whole tissue associations are cultivated. Though many cells keep their ability to ... Cell. 154 (4): 737-747. doi:10.1016/j.cell.2013.07.030. PMC 3950538 . PMID 23953109. ... It was given the name kinetin because of its ability to induce cell division, provided that auxin was present in the medium. ...
Eddy B, Borman G, Berkeley W, Young R (1961). "Tumors induced in hamsters by injection of rhesus monkey kidney cell extracts". ... Oral polio vaccine (OPV) is an attenuated vaccine, produced by the passage of the virus through non-human cells at a sub- ... In 1960, it was determined that the rhesus monkey kidney cells used to prepare the poliovirus vaccines were infected with the ... Carbone M (1999). "Simian virus 40 and human tumors: It is time to study mechanisms". J Cell Biochem. 76 (2): 189-93. doi: ...
Keil, C.; Petermann, E.; Oei, S. L. (2004). "Tannins elevate the level of poly(ADP-ribose) in HeLa cell extracts". Archives of ... Cell Genet. 85 (3-4): 269-70. doi:10.1159/000015310. PMID 10449915. Affar, E. B.; Germain, M.; Winstall, E.; Vodenicharov, M.; ... Experimental Cell Research. 297 (2): 521-532. doi:10.1016/j.yexcr.2004.03.050. PMID 15212953. Putt, K. S.; Hergenrother, P. J ... glycohydrolase in mammalian cells". Biochemical and Biophysical Research Communications. 307 (4): 915-921. doi:10.1016/S0006- ...
"Quantitative assay of senescence-associated beta-galactosidase activity in mammalian cell extracts". Anal. Biochem. 343 (2): ... "Quantitative assay of senescence-associated β-galactosidase activity in mammalian cell extracts". Analytical Biochemistry. 343 ... However, when DNA fragments are inserted in the vector, the production of LacZα is disrupted, the cells therefore show no β- ... Nevertheless, it remains the most widely used biomarker for senescent and aging cells, because it is reliable and easy to ...
CGAS-STING cytosolic DNA sensing pathway
Through biochemical fractionation of cell extracts and quantitative mass spectrometry, Sun, et al. identified cGAS as the DNA- ... cGAMP was discovered by James Chen and colleagues by collecting cytoplasmic extracts from cells transfected with different ... and T cells. In many tumor cells, the DNA damage response is constitutively active, leading to the accumulation of cytoplasmic ... In normal cells, nucleic acid is confined to the nucleus or mitochondria. The presence of nucleic acid in the cytosol is ...
"Quantitative assay of senescence-associated beta-galactosidase activity in mammalian cell extracts". Anal. Biochem. 343 (2): ... "SA-β-galactosidase expressing cells from human renal proximal tubular epithelial cells". www.sciencellonline.com. Archived from ... However, it remains as the most widely used biomarker for senescent and aging cells, because it is easy to detect and reliable ... following the observation that when beta-galactosidase assays were carried out at pH 6.0, only cells in senescence state ...
Rolling circle replication
"Rolling circle replication of human papillomavirus type 16 DNA in epithelial cell extracts". Genes to Cells. 16 (1): 23-33. doi ... The virus has a circular, single stranded, DNA that replicates in host plant cells. The entire process is initiated by the ... HPV-16 infects human epithelial cells and has a double stranded circular genome. During replication, at the origin, the E1 ... Some DNA viruses replicate their genomic information in host cells via rolling circle replication. For instance, human ...
Wackett, L. P.; Gibson, D. T. (1982). "Metabolism of xenobiotic compounds by enzymes in cell extracts of the fungus ...
The second type of experiment consisted of in vitro experiments done on cell extracts. Laboratory synthesis of ICZs has been a ... with special attention paid to cancer cell effects. The general modes of action found in mammalian cells are inhibition of ... Two of the more important ones to date have been Arcyriacyanin A, which was found to inhibit a panel of human cancer cells by ... Because of this, it is thought that most of the anti-cell growth function of ICZs comes from inhibition of various protein ...
... formulas also contain stem cells extracted from Nicotiana sylvestris plant. Together, along with several other ... The skin care line's main ingredient contains stem cells from the Alpine Rose flower. The Alpine Rose is a small pink flower ... vbeauté is a skin care line based on advanced botanical technology using plant stem cell complexes. Julie Macklow launched ... botanical ingredients, these plant extracts combine to form a complex compound which has anti-aging and protective properties. ...
The crude cell extracts of all Equisetum species tested contain mixed-linkage glucan : Xyloglucan endotransglucosylase (MXE) ... Due to the correlation between MXE activity and cell age, MXE has been proposed to promote the cessation of cell expansion.[ ... An extract is often used to provide silica for supplementation. Horsetail was often used by Indians to polish wooden tools. ... Extracts and other preparations of E. arvense have served as herbal remedies, with records dating to ancient Greek and Roman ...
Estrogen receptor alpha
Studies in cell extracts and intact cells and their implications for hormone-dependent transcriptional activation". J. Biol. ... "Interaction of Vault Particles with Estrogen Receptor in the MCF-7 Breast Cancer Cell". J. Cell Biol. 141 (6): 1301-10. doi: ... Cell. Biol. 19 (8): 5363-72. PMC 84379 . PMID 10409727. Bu H, Kashireddy P, Chang J, Zhu YT, Zhang Z, Zheng W, Rao SM, Zhu YJ ( ... Cell. 3 (6): 903-10. doi:10.1016/S1534-5807(02)00360-X. PMID 12479814. Ogawa S, Inoue S, Watanabe T, Hiroi H, Orimo A, Hosoi T ...
Small nucleolar RNA SNORA40
2004) from a HeLa cell extract immunoprecipitated with an anti-GAR1 antibody. It is predicted to guide the pseudouridylation of ...
These have been found in cell-extracted degradosome preparations from E. coli. The structure of RNA degradosome is not as rigid ... Even in resting cells, RNA is degraded in a steady state, and the nucleotide products of this process are later reused for ... This muli-protein complex is stimulated by a non-coding RNA, called miRNA in Eukaryotic cells and sRNA in bacteria. Small ... The store of cellular RNA in the cells is constantly fluctuating. For example, in Escherichia coli, Messenger RNA's life ...
Small nucleolar RNA SNORA53
2004) from a HeLa cell extract immunoprecipitated with an anti-GAR1 antibody. It has no identified target RNA. RNA residues ...
"Pyrogallol-to-phloroglucinol conversion and other hydroxyl-transfer reactions catalyzed by cell extracts of Pelobacter ...
... terminal phosphate cyclase activity and RNA ligation in HeLa cell extract". Nucleic Acids Res. 11 (5): 1405-18. doi:10.1093/nar ...
Cell-free protein synthesis
A cell extract is obtained by lysing the cell of interest and centrifuging out the cell walls, DNA genome, and other debris. ... Common cell extracts in use today are made from E. coli (ECE), rabbit reticulocytes (RRL), wheat germ (WGE), and insect cells ( ... Common components of a cell-free reaction include a cell extract, an energy source, a supply of amino acids, cofactors such as ... Most notably, a cell-free reaction, including extract preparation, usually takes 1 -2 days, whereas in vivo protein expression ...
"Protein phosphorylation regulated by cyclic nucleotide-dependent protein kinases in cell extracts and in intact human ... proteins and the Arp2/3 complex link T cell receptor (TCR) signaling to the actin cytoskeleton". J. Cell Biol. 149 (1): 181-94 ... Cell. 11 (1): 117-29. doi:10.1091/mbc.11.1.117. PMC 14761 . PMID 10637295. Krause M, Sechi AS, Konradt M, et al. (2000). "Fyn- ... VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may ...
Centre for Genomic Regulation
The investigators have proposed a dual approach, combining in vitro and cell extract techniques. The goal is to gain knowledge ... Cell polarity is a prerequisite for several fundamental operations in animal cells, such as asymmetric cell division and ... Cell and Developmental Biology, or CDB, is built on the idea that the cell should be the primary focus of developmental biology ... Stem cell research includes differentiation and transdifferentiation in the hematopoietic system, somatic cell reprogramming, ...
Ariosa v. Sequenom
6,258,540, which claims methods of using cell-free fetal DNA (cffDNA) circulating in maternal plasma (cell-free blood) to ... "The claimed method is a new process of detecting cffDNA by devising a novel sample source from which to extract it, namely, ...
OCIAD1兔多克隆抗体(ab91574)可与人样本反应并经WB, ELISA, ICC/IF实验严格验证。中国75%以上现货，所有产品均提供质保服务，可通过电话、电邮或微信获得本地专属技术支持。
Kairos Web Brasil / Pre os de medicamentos: Broncho-vaxom (Lisado bacteriano + Diplococcus pneumoniae + Haemophilus influenzae ...
Pre o de Broncho-vaxom, laborat rio Takeda, droga: Lisado bacteriano + Diplococcus pneumoniae + Haemophilus influenzae + Klebsiella ozanae + Klebsiella pneumoniae + Neisseria catarrhalis + Staphylococcus aureus + Streptococcus. Encontre pre os atualizados de todos os medicamentos / rem dios / princ pios ativos comerciais e gen ricos.
Clearsate.jpg CLEARSATE IS:A potent platelet lysate product to be used as a media supplement alternative to fetal bovine serum (FBS)Produced from expired human platelets and able to be used without an anticoagulantTriple-filter
Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton...
TY - JOUR. T1 - Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton jelly mesenchymal stromal cells. AU - Chen, Ming Sheng. AU - Wang, Tsung Jen. AU - Lin, Hsiu Chen. AU - Thierry, Burnouf. PY - 2019/3/25. Y1 - 2019/3/25. N2 - There is accumulating experimental evidence that human platelet lysate (HPL) made from platelet concentrates can replace fetal bovine serum (FBS) as a xeno-free clinical-grade supplement of growth media to expand mesenchymal stromal cells (MSCs). However, uncertainties exist in regard to impacts that various manufacturing methods of HPL can exert on the expansion and differentiation capacity of MSCs. In particular, there is a need to evaluate the possibility of implementing virus-inactivation treatment during HPL production to ensure optimal safety of industrial HPL pools. Expired human platelet concentrates from four different donors were pooled and subjected to freeze-thaw cycles (-80/+37 °C), ...
Like OCIAD1, OCIAD2 is a cancer-related protein and its expression level increases during the course of malignant progression and is thought to be a very useful marker for evaluating the malignancy of ovarian mucinous tumors ...
MCF7 whole cell lysate Lysates datasheet (ab29537). Abcam offers quality products including antibodies, assays and other reagents.
Currently Unavailable Frog eggs: Rana genus. The actual species may vary depending on what is available at the time. We will ship as available in early spring. Per/clump. …
Alternative to Spine Surgery and Epidural Steroid Injections | Center for Regenerative Orthopedic Medicine, PC
Regenexx Platelet Lysate can offer relief from lower back or neck pain, buttock and leg pain, or numbness and tingling in the arms, hands, legs or feet. Its a safe, non-surgical treatment alternative for patients suffering from bulging discs and herniated cervical, thoracic, or lumbar disc related problems.. Most patients with bulging or herniated discs respond well to a platelet lysate injection into the space surrounding the inflamed spinal nerves. Other clinics resort to an injection of stems cells directly into a patients disc. While this can sometimes ultimately be necessary, our platelet lysate procedure is less invasive and lower risk. Nobody other than Regenexx offers this type of procedure, which utilizes natural growth factors produced from the patients own blood platelets. Regenexx is the only provider with experience in both stem cells and platelet lysate so we are uniquely able to determine the best treatment options to address your source of pain. ...
HeLa whole cell lysate Lysates datasheet (ab29545). Abcam offers quality products including antibodies, assays and other reagents.
ProSci offers more than 700 cell lysates that have been rigorously tested for your research projects. Order your research lysates online from ProSci today.
ProSci offers more than 700 cell lysates that have been rigorously tested for your research projects. Order your research lysates online from ProSci today.
Myc-DDK-tagged ORF clone of Homo sapiens OCIA domain containing 1 (OCIAD1), transcript variant 4 as transfection-ready DNA -...
Myc-DDK-tagged ORF clone of Homo sapiens OCIA domain containing 1 (OCIAD1), transcript variant 4 as transfection-ready DNA - 10 µg - OriGene - cdna clones
494; 1536; L895; S425; S424; 1048; T048; L030; 103; S175; S468; S832; S831; Q888; M365; CA19; 684; CA27; 698; Q620; 6050; M301; PCAN; X621; 130; 383; X587; 977; 1460; 0616; 0618; VAP1; ACT4; S602; C17; C12; C15; X860; T281; 1565; L067; L379; 2560; L529; T080; Q371; 433; 1717; 20; 21; NK57; CD57; 1065; 1532; CENP; 392; SMAC; CM24; 6520; 6513; 6510; S006; T261; T039; Q258; 135; S488; A870; 109; C1ES; X599; A125; Q469; S503; 468; V111; 4962; T449; M162; CDIF; 4964; T046; A867; S994; COC; 485; X605; X601; L251; 478; S240; 551; 0464; 0459; 666; 1978; Q240; 12; 2676; A937; T112; 950; S513; U204; S239; X328; S728; S726; T358; A322; A462; T228; 572; S867; M273; 110; CPKI; 712; S252; S120; 112; 497A; 2007; CRYO; Y224; A006; M371; N394; URCS; BFCG; 6011; EYCU; FGCO; FGCL; GENC; S491; Q602; NASC; SINC; S150; 7457; THAB; WOUC; L776; YEAS; YSTC; 2003; 7962; BLCU; EACU; STCU; A604; URCU; A900; 1074; 543; 5091; 5092; 5705; X624; CF60; X622; X613; Q190; X708; 619; 546; CYTI; NGYN; S410; S412; Q640; WODC; 306; L502;
Fibroblast growth factor 2 and platelet-derived growth factor, but not platelet lysate, induce proliferation-dependent,...
FGF-2 and, to a lesser extent, PDGF-BB induced in adult human MSCs the expression of HLA-DR (normally induced by inflammatory cytokines), which was able to stimulate CD4+ T cells via superantigen binding. In contrast to IFNγ, FGF induced HLA-DR expression only in human MSCs proliferating under its mitogenic effect and not in mouse MSCs or in differentiated human cells. Although it induced cell proliferation, human platelet lysate did not cause HLA-DR expression in human MSCs. HLA-DR expression occurred following FGF-specific binding to its receptor(s), mainly FGF receptor 1, without inducing IFNγ or tumor necrosis factor α expression. Both MAPK/ERK-1/2 and phosphatidylinositol 3-kinase/Akt controlled cell proliferation and HLA-DR expression, but only MAPK/ERK-1/2 controlled the induction of the class II MHC transcription activator protein CIITA, the major determinant of HLA-DR transcription. ...
Purified Recombinant Human PLIN2 293 Cell Lysate from Creative Biomart. Recombinant Human PLIN2 293 Cell Lysate can be used for research.
TY - JOUR. T1 - Whats hot in the prostate?. AU - Gleave, Martin. AU - Evans, Christopher P.. PY - 2003. Y1 - 2003. UR - http://www.scopus.com/inward/record.url?scp=0347268981&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0347268981&partnerID=8YFLogxK. U2 - 10.1038/sj.pcan.4500699. DO - 10.1038/sj.pcan.4500699. M3 - Article. C2 - 14663465. AN - SCOPUS:0347268981. VL - 6. SP - 268. EP - 271. JO - Prostate Cancer and Prostatic Diseases. JF - Prostate Cancer and Prostatic Diseases. SN - 1365-7852. IS - 4. ER - ...
OK, well thanks to everyones advice we now have a clean and clear swimming pool. However, when we initially shocked the pool it was basically a mass murder on the local frog population.. We probably pulled out 4 or 500 dead tadpoles and close to 20 full grown frogs. We thought we had wiped them out, but we were wrong... Every morning I check the pool out and skim it and every morning I see all of these little black dots that are everywhere. At first I thought it was just dirt, but after
Nuclear assembly is independent of linker histones.: The role of linker histones in the assembly of functional nuclei was examined with the use of a cell-free e
This image is part of a large data set of Xenopus laevis eggs imaged at various times post fertilization (the first number of the file name correspond...
OM-85 treatment increases polyclonal antibody production with the capacity to neutralize influenza infection. BALB/c mice were treated with OM-85 or control sol
Purified Recombinant Human AGXT 293 Cell Lysate from Creative Biomart. Recombinant Human AGXT 293 Cell Lysate can be used for research.
Copyright © 2020 The President and Fellows of Harvard College , Accessibility , Digital Accessibility , Report Copyright Infringement ...
PatientsLikeMe | Animal Embryo Cell Extracts report for patients like you
Find the most comprehensive real-world treatment information on Animal Embryo Cell Extracts at PatientsLikeMe. 0 patients with ... bipolar I disorder or psoriasis currently use Animal Embryo Cell Extracts. ... What is Animal Embryo Cell Extracts?. Category: Others false Animal Embryonic Live Cell Therapy uses liquid extracts obtained ... Stopped using Animal Embryo Cell Extracts Duration. Patients. This item is relevant to you: 1 - 6 months 1 * 1 ...https://www.patientslikeme.com/treatments/show/5515-animal-embryo-cell-extracts-side-effects-and-efficacy
Cancer cells extracted from blood | Health24
An experimental process that snags lung cancer cells from a blood sample could give doctors real-time feedback on the most ... Cancer cells extracted from blood. An experimental process that snags lung cancer cells from a blood sample could give doctors ... In December, the same group reported in Nature that their circulating tumour cells, or CTC, chip could extract malignant cells ... and Harvard Medical School and colleagues were able to extract blood-borne cancer cells from 27 volunteers with non-small-cell ...https://www.health24.com/Medical/Archive/Cancer-cells-extracted-from-blood-20120721
Doctor Utilizes Stem Cell Extracts with Promisi... ((PRWEB) August 30 2013 Dr. Ann J Pete...)
The liquid stem cells come from a frozen st,Doctor,Utilizes,Stem,Cell,Extracts,with,Promising,Results,medicine,medical news ... Ann J Peters internationally recognized anti-aging specialist has found significant success utilizing liquid stem cells for ... The liquid stem cells come from a frozen stem cell extract that is consumed orally. This comes as good news to millions of ... Doctor Utilizes Stem Cell Extracts with Promising Results. (PRWEB) August 30 2013 Dr. Ann J Peters internationally recognized ...http://www.bio-medicine.org/medicine-news-1/Doctor-Utilizes-Stem-Cell-Extracts-with-Promising-Results-115198-1/
In vitro repair of complex unligatable oxidatively induced DNA double-strand breaks by human cell extracts. - PubMed - NCBI
In vitro repair of complex unligatable oxidatively induced DNA double-strand breaks by human cell extracts.. Pastwa E1, Neumann ... In vitro repair of complex unligatable oxidatively induced DNA double-strand breaks by human cell extracts ... In vitro repair of complex unligatable oxidatively induced DNA double-strand breaks by human cell extracts ... In vitro repair of complex unligatable oxidatively induced DNA double-strand breaks by human cell extracts ...https://www.ncbi.nlm.nih.gov/pubmed/11504886?dopt=Abstract
GFP assay in cell-free extracts?
... Satoshi Naito naito at abs.agr.hokudai.ac.jp Wed Nov 11 19:13:37 EST 1998 *Previous message: ... Dear Netters, Is there anyone who assayed GFP fluorescence in plant/cell extracts ...http://www.bio.net/bionet/mm/arab-gen/1998-November/006717.html
Interpreting impedance spectra of organic photovoltaic cells-Extracting charge transit and recombination rates (Journal Article...
Journal Article: Interpreting impedance spectra of organic photovoltaic cells-Extracting charge transit and recombination rates ... Title: Interpreting impedance spectra of organic photovoltaic cells-Extracting charge transit and recombination rates ... Extracting the transit and recombination rate constants permits a detailed understanding of how OPV architecture and processing ... spectroscopy has been widely used to extract the electron-hole recombination rate constant in organic photovoltaic cells (OPVs ...https://www.osti.gov/scitech/biblio/22305731-interpreting-impedance-spectra-organic-photovoltaic-cellsextracting-charge-transit-recombination-rates
Differentiation of Helicobacter pyloriIsolates Based on Lectin Binding of Cell Extracts in an Agglutination Assay | Journal of...
Differentiation of Helicobacter pyloriIsolates Based on Lectin Binding of Cell Extracts in an Agglutination Assay. Sean O. ... Protein concentration.Protein concentrations of whole-cell samples and extracts were determined with a commercial assay kit ( ... Differentiation of Helicobacter pyloriIsolates Based on Lectin Binding of Cell Extracts in an Agglutination Assay ... Differentiation of Helicobacter pyloriIsolates Based on Lectin Binding of Cell Extracts in an Agglutination Assay ...https://jcm.asm.org/content/37/6/1994
Proof of concept for microarray-based detection of DNA-binding oncogenes in cell extracts - Infoscience
This approach was designed to determine the DNA-binding activity of proteins in crude cell extracts, complementing conventional ... The microarray is also of high sensitivity, as it allows the detection of a rare DNA-binding protein from breast cancer cells, ... Proof of concept for microarray-based detection of DNA-binding oncogenes in cell extracts Egener, T. ; Roulet, E. ; Zehnder, M. ... This approach was designed to determine the DNA-binding activity of proteins in crude cell extracts, complementing conventional ...https://infoscience.epfl.ch/record/114904/?ln=fr
Phosphonoacetate biosynthesis: in vitro detection of a novel NADP(+)-dependent phosphonoacetaldehyde-oxidizing activity in cell...
A novel phosphonoacetaldehyde-oxidizing activity was detected in cell-extracts of the marine bacterium Roseovarius nubinhibens ... dependent phosphonoacetaldehyde-oxidizing activity in cell-extracts of the marine Roseovarius nubinhibens ISM.. [N A Cooley, A ... ISM grown on 2-aminoethylphosphonic acid (2-AEP; ciliatine). Extracts also contained 2-AEP transaminase and phosphonoacetate ...https://www.sigmaaldrich.com/catalog/papers/21861368
Extract cell comments using Excel Formula
Any way to extract a comment to a cell WITHOUT using VBA? Unfortunately MS extracted the VBA utility from my Excel For Mac 2008 ... You could also send the file to a Windows user and get the comments extracted to cells with some simple VBA. Sub Extract_ ... is there a way to have Excel randomly select a cell from an array and duplicate that cell in another cell, INCLUDING any ... But what if you have typed a ton of cell comments and now want a way to extract them and do something with that data? ...https://chandoo.org/wp/get-cell-comments/
Swertia Chirata Stem Cell Extract | Temple Spa
Swertia Chirata Stem Cell Extract An ultra-high tech ingredient that is used in Indian medicine for its healing properties. The ...https://www.templespa.com/swertia-chirata-stem-cell-extract
AID 675297 - Inhibition of HDAC in human HeLa cell extracts using Fluor de Lys as substrate at 0.1 uM by fluorescence assay -...
Inhibition of HDAC in human HeLa cell extracts using Fluor de Lys as substrate at 0.1 uM by fluorescence assay. ...https://pubchem.ncbi.nlm.nih.gov/bioassay/675297
RNA-Binding Protein Immunoprecipitation from Whole-Cell Extracts
A whole-cell extract is subjected to immunoprecipitation with an antibody against the GFP tag. In parallel, a mock ... RNA-Binding Protein Immunoprecipitation from Whole-Cell Extracts. Köster T, Staiger D (2014) Methods in molecular biology ( ... RNA-Binding Protein Immunoprecipitation from Whole-Cell Extracts. Methods in molecular biology (Clifton, N.J.), 1062, p 679-695 ... Köster T, Staiger D. RNA-Binding Protein Immunoprecipitation from Whole-Cell Extracts. Methods in molecular biology (Clifton, N ...https://pub.uni-bielefeld.de/publication/2629103
Bag cell extract inhibits tail-siphon withdrawal reflex, suppresses long-term but not short-term sensitization, and attenuates...
To address this issue, we examined the effects of an extract (bag cell extract, BCE) that contained BCPs, as well as egg-laying ... Bag cell extract inhibits tail-siphon withdrawal reflex, suppresses long-term but not short-term sensitization, and attenuates ... Bag cell extract inhibits tail-siphon withdrawal reflex, suppresses long-term but not short-term sensitization, and attenuates ... Bag cell extract inhibits tail-siphon withdrawal reflex, suppresses long-term but not short-term sensitization, and attenuates ...http://www.jneurosci.org/content/13/4/1688
Reduction of Silver Ions by Cell Free Extracts of Westiellopsis sp.
... P. T. V. Lakshmi,1,2 Devi Priyanka,1 and A. Annamalai3 ...https://www.hindawi.com/journals/ijbm/2015/539494/abs/
Differential regulation of iron regulatory element-binding protein(s) in cell extracts of activated lymphocytes versus...
The cells were grown in either standard or iron-supplemented culture. TfR and ferritin expression was evaluated at both the ... It is noteworthy the effect of beta-mercaptoethanol is cell specific, i.e. the ratio of total versus spontaneous IRE-BP ... Both the rise of TfR and spontaneous IRE-BP activity are completely inhibited in iron-supplemented T cell cultures. In contrast ... expression in cells requiring iron for their proliferation, in contrast to the positive feedback observed in monocytes- ...https://infoscience.epfl.ch/record/117559
Nissan's Fuel-Cell Car Extracts Hydrogen from Biofuel - News - Car and Driver.
Nissan is testing a different kind of fuel-cell electric car that extracts hydrogen onboard from biofuel, such as ethanol. Read ... Nissans Fuel-Cell Car Hits the Road in Brazil, Extracts Hydrogen from Biofuel. ... Hydrogen Stations Needed for Fuel-Cell Vehicles of the Future * Toyota Details 2015 Hydrogen Fuel-Cell Vehicle: 300-Plus-Miles ... Unlike the fuel cells used by the Honda Clarity, the Toyota Mirai, and the Hyundai Tucson, which convert hydrogen and air to ...https://www.caranddriver.com/news/a15345839/nissans-fuel-cell-car-hits-the-road-in-brazil-extracts-hydrogen-from-bio-fuel/
Chapter 9 (2) - Chapter 9-Avery MacLeon and McCarty What is the transforming material o Cell extracts treated with protein...
... o Cell extracts treated with protein, carbohydrate, and RNA ... o Cell extracts treated with protein, carbohydrate, and RNA ... Binary Fission The Eukaryotic Cell Cycle Figure 11 3 Two Types of Cell Division ... digesting enzymes could still transform bacteria o Cell extracts treated with DNA-digesting enzymes lost their transforming ...https://www.coursehero.com/file/6855855/Chapter-9-2/
Text Toolkit for Excel - remove spaces, split cells, extract substrings, and more
... split cells, remove line breaks, punctuation marks and other symbols, extract substring, change case, convert numbers formatted ... Try the Extract Text utility. It can pull any number of first or last characters from a cell. Also, you can extract a substring ... add the same text to the beginning or end of all cells in a range, convert text to numbers, split cells, extract substrings, ... How to split cells is Excel? Have a look at the Split Text utility. It splits cells by character, mask, or strings. Choose how ...https://www.ablebits.com/excel-clean-cells/index.php?tool=SplitText
Text Toolkit for Excel - remove spaces, split cells, extract substrings, and more
... split cells, remove line breaks, punctuation marks and other symbols, extract substring, change case, convert numbers formatted ... Try the Extract Text utility. It can pull any number of first or last characters from a cell. Also, you can extract a substring ... add the same text to the beginning or end of all cells in a range, convert text to numbers, split cells, extract substrings, ... How to split cells is Excel? Have a look at the Split Text utility. It splits cells by character, mask, or strings. Choose how ...https://www.ablebits.com/excel-clean-cells/index.php?tool=TrimSpaces
Activity of a Chick Collagen Gene in Heterologous and Homologous Cell-Free Extracts | SpringerLink
Activity of a Chick Collagen Gene in Heterologous and Homologous Cell-Free Extracts. ... Activity of a Chick Collagen Gene in Heterologous and Homologous Cell-Free Extracts. In: Kumar A. (eds) Eukaryotic Gene ... Hansen, U., Tenen, D. G., Livington, D. M., and Sharp, P. A., 1981, Cell 27: 603-612.PubMedCrossRefGoogle Scholar ... Weil, P. A., Luse, D. S., Segall, J., and Roeder, R. G., 1979, Cell 18: 469-484.PubMedCrossRefGoogle Scholar ...https://link.springer.com/chapter/10.1007/978-1-4684-7459-6_10
Biosynthesis of beta-glucans by cell-free extracts from Saccharomyces cerevisiae.
Cell-free extracts from Saccharomyces cerevisiae catalyzed the incorporation of glucosyl residues from UDP-[U-14C]glucose into ... Cell-free extracts from Saccharomyces cerevisiae catalyzed the incorporation of glucosyl residues from UDP-[U-14C]glucose into ... 22355 - Biosynthesis of beta-glucans by cell-free extracts from saccharomyces cerevisiae.. 391565 - A novel atp-driven glucose ... Cell Cycle. Cell-Free System. Glucosyltransferases / metabolism. Glycogen Synthase / metabolism. Hydrogen-Ion Concentration. ...http://www.biomedsearch.com/nih/Biosynthesis-beta-glucans-by-cell/22355.html
Cell Extracts | Colorado PROFILES
"Cell Extracts" by people in this website by year, and whether "Cell Extracts" was a major or minor topic of these publications ... "Cell Extracts" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Studying the composition of mRNPs in vitro using splicing-competent cell extracts. Methods. 2014 Feb; 65(3):342-9. ... Below are the most recent publications written about "Cell Extracts" by people in Profiles. ...https://profiles.ucdenver.edu/display/218518
A tomato stem cell extract, containing antioxidant compounds and metal chelating factors, protects skin cells from heavy metal...
A tomato stem cell extract, containing antioxidant compounds and metal chelating factors, protects skin cells from heavy metal- ... To protect human skin cells from heavy metal toxicity, we developed a new cosmetic active ingredient from Lycopersicon ... This product, besides its high content of antioxidant compounds, contained PC, effective in the protection of skin cells ... Plants have evolved sophisticated mechanisms to protect their cells from heavy metal toxicity, including the synthesis of metal ...https://insights.ovid.com/ijcos/201112000/00013257-201112000-00010
BRAF protein immunoprecipitation, elution, and digestion from cell extract using a microfluidic mixer for mutant BRAF protein...
This study utilized a microfluidic mixer for the sample pretreatment of cell extracts for target protein quantification by mass ... Whole cell lysates of the cancer cell line HT-29 with gene mutations were used as the sample. The target protein BRAF was ... Three micromixers were used to reduce the sample pretreatment time of cell extracts for target protein quantification by mass ... This study utilized a microfluidic mixer for the sample pretreatment of cell extracts for target protein quantification by mass ...https://rd.springer.com/article/10.1007%2Fs00216-018-1536-2
- We describe a new assay for in vitro repair of oxidatively induced DNA double-strand breaks (DSBs) by HeLa cell nuclear extracts. (nih.gov)
- Coommassie stained SDS-PAGE of 25 ?g of Doxorubicin stimulated Human Derived Hela Cell Nuclear Extract (Ready-to-Use) separated in a 4-20% gradient gel under non-reducing conditions. (antikoerper-online.de)
- In vitro repair of complex unligatable oxidatively induced DNA double-strand breaks by human cell extracts. (nih.gov)
- Phosphonoacetate biosynthesis: in vitro detection of a novel NADP(+)-dependent phosphonoacetaldehyde-oxidizing activity in cell-extracts of the marine Roseovarius nubinhibens ISM. (sigmaaldrich.com)
- Studying the composition of mRNPs in vitro using splicing-competent cell extracts. (ucdenver.edu)
- Lasda EL, Kuersten S, Blumenthal T. SL trans-splicing in a Caenorhabditis elegans in vitro extract. (ucdenver.edu)
- Animal Embryonic Live Cell Therapy uses liquid extracts obtained from porcine mesenchyme, liver, and central nervous system tissues. (patientslikeme.com)
- In this paper, we extend an earlier mathematical model of embryonic cell cycles to include experimentally motivated roles for PP2A:B55δ and its regulation by Greatwall kinase. (nih.gov)
- Our model is consistent with what is already known about the regulation of CDK and PP2A:B55δ in frog eggs, and it suggests a previously undescribed role for the Greatwall-PP2A:B55δ interaction in creating a toggle switch for activation of the anaphase-promoting complex as embryonic cells exit mitosis and return to interphase. (nih.gov)
- PSC ® Distribution LLC is world renowned for the distribution of quality embryonic plant extracts. (epsce.com)
- PRWEB) August 30 2013 Dr. Ann J Peters internationally recognized anti-aging specialist has found significant success utilizing liquid stem cells for both the rejuvenation of damaged organs and for general anti-aging purposes. (bio-medicine.org)
- Dr. Ann J Peters, internationally recognized anti-aging specialist, has found significant success utilizing liquid stem cells for both the rejuvenation of damaged organs and for general anti-aging purposes. (bio-medicine.org)
- The liquid stem cells come from a frozen stem cell extract that is consumed orally. (bio-medicine.org)
- Stem cells represent probably some of the most promising innovations in medicine today," said Dr. Peters. (bio-medicine.org)
- Anyone can benefit from liquid stem cells, from young children to adults. (bio-medicine.org)
- Entire industries have blossomed around the use of stem cells, notably its use in sports medicine. (bio-medicine.org)
- Clinicians continually discover new uses for stem cells, from the repair of damaged or failing organs, damaged tissue, and diseases, such as Type I diabetes. (bio-medicine.org)
- The umbilical cord of a newborn contains a large amount of the baby's own stem cells, which can be effectively stored for future use. (bio-medicine.org)
- Dr. Peters recently visited a clinic in Switzerland that utilizes similar liquid stem cells extracts to the ones she uses in her practices in San Francisco, New York and Palm Beach. (bio-medicine.org)
- To protect human skin cells from heavy metal toxicity, we developed a new cosmetic active ingredient from Lycopersicon esculentum (tomato) cultured stem cells. (ovid.com)
- Have higher concentrations of phytochemicals present in nanoparticle size, better for osmosis into the nucleus of cells and tissues. (epsce.com)
- Antinuclear antibodies (ANAs, also known as antinuclear factor or ANF) are autoantibodies that bind to contents of the cell nucleus. (wikipedia.org)
- They are termed ENA because they can be extracted from the cell nucleus with saline. (wikipedia.org)
- An experimental process that snags lung cancer cells from a blood sample could give doctors real-time feedback on the most effective therapy, researchers reported. (health24.com)
- The study, reported on the Web site of the New England Journal of Medicine , is another step in the quest for individualised medicine, where doctors strive to quickly assess a tumour, choose the most effective treatment, and alter that treatment as cancer cells adapt. (health24.com)
- Unlike the fuel cells used by the Honda Clarity , the Toyota Mirai , and the Hyundai Tucson , which convert hydrogen and air to electricity, the SOFC design contains lower-cost ceramic-based materials instead of expensive platinum catalysts and operates at higher temperatures. (caranddriver.com)
- Looking for a quick way to remove extra spaces and unwanted characters, change text case, count characters and words, add the same text to the beginning or end of all cells in a range, convert text to numbers, split cells, extract substrings, replace irrelevant symbols with the right ones? (ablebits.com)
- Although the mechanisms by which cells sense and convert mechanical force into intracellular biochemical signals have been a subject of keen interest, the problem is not easy to approach experimentally. (rupress.org)
- This graph shows the total number of publications written about "Cell Extracts" by people in this website by year, and whether "Cell Extracts" was a major or minor topic of these publications. (ucdenver.edu)
- Below are the most recent publications written about "Cell Extracts" by people in Profiles. (ucdenver.edu)