A telecommunication system combining the transmission of a document scanned at a transmitter, its reconstruction at a receiving station, and its duplication there by a copier.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
Analog or digital communications device in which the user has a wireless connection from a telephone to a nearby transmitter. It is termed cellular because the service area is divided into multiple "cells." As the user moves from one cell area to another, the call is transferred to the local transmitter.
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
A syndrome characterized by persistent or recurrent fatigue, diffuse musculoskeletal pain, sleep disturbances, and subjective cognitive impairment of 6 months duration or longer. Symptoms are not caused by ongoing exertion; are not relieved by rest; and result in a substantial reduction of previous levels of occupational, educational, social, or personal activities. Minor alterations of immune, neuroendocrine, and autonomic function may be associated with this syndrome. There is also considerable overlap between this condition and FIBROMYALGIA. (From Semin Neurol 1998;18(2):237-42; Ann Intern Med 1994 Dec 15;121(12): 953-9)
An acute, febrile, infectious disease generally occurring in epidemics. It is usually caused by coxsackieviruses B and sometimes by coxsackieviruses A; echoviruses; or other enteroviruses.
A major affective disorder marked by severe mood swings (manic or major depressive episodes) and a tendency to remission and recurrence.
A behavior disorder originating in childhood in which the essential features are signs of developmentally inappropriate inattention, impulsivity, and hyperactivity. Although most individuals have symptoms of both inattention and hyperactivity-impulsivity, one or the other pattern may be predominant. The disorder is more frequent in males than females. Onset is in childhood. Symptoms often attenuate during late adolescence although a minority experience the full complement of symptoms into mid-adulthood. (From DSM-V)
A degenerative disorder affecting upper MOTOR NEURONS in the brain and lower motor neurons in the brain stem and SPINAL CORD. Disease onset is usually after the age of 50 and the process is usually fatal within 3 to 6 years. Clinical manifestations include progressive weakness, atrophy, FASCICULATION, hyperreflexia, DYSARTHRIA, dysphagia, and eventual paralysis of respiratory function. Pathologic features include the replacement of motor neurons with fibrous ASTROCYTES and atrophy of anterior SPINAL NERVE ROOTS and corticospinal tracts. (From Adams et al., Principles of Neurology, 6th ed, pp1089-94)
Retrograde flow of gastric juice (GASTRIC ACID) and/or duodenal contents (BILE ACIDS; PANCREATIC JUICE) into the distal ESOPHAGUS, commonly due to incompetence of the LOWER ESOPHAGEAL SPHINCTER.
A disorder with chronic or recurrent colonic symptoms without a clearcut etiology. This condition is characterized by chronic or recurrent ABDOMINAL PAIN, bloating, MUCUS in FECES, and an erratic disturbance of DEFECATION.
Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.
A specialized CONNECTIVE TISSUE that is the main constituent of the SKELETON. The principle cellular component of bone is comprised of OSTEOBLASTS; OSTEOCYTES; and OSTEOCLASTS, while FIBRILLAR COLLAGENS and hydroxyapatite crystals form the BONE MATRIX.
Fats present in food, especially in animal products such as meat, meat products, butter, ghee. They are present in lower amounts in nuts, seeds, and avocados.
The study of natural phenomena by observation, measurement, and experimentation.
The glyceryl esters of a fatty acid, or of a mixture of fatty acids. They are generally odorless, colorless, and tasteless if pure, but they may be flavored according to origin. Fats are insoluble in water, soluble in most organic solvents. They occur in animal and vegetable tissue and are generally obtained by boiling or by extraction under pressure. They are important in the diet (DIETARY FATS) as a source of energy. (Grant & Hackh's Chemical Dictionary, 5th ed)
Cells in the body that store FATS, usually in the form of TRIGLYCERIDES. WHITE ADIPOCYTES are the predominant type and found mostly in the abdominal cavity and subcutaneous tissue. BROWN ADIPOCYTES are thermogenic cells that can be found in newborns of some species and hibernating mammals.
A genus of perennial herbs used topically and internally. It contains echinacoside, GLYCOSIDES; INULIN; isobutyl amides, resin, and SESQUITERPENES.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
The effect of herbs, other PLANTS, or PLANT EXTRACTS on the activity, metabolism, or toxicity of drugs.
The above-ground plant without the roots.
Receptors that are specifically found on the surface of NATURAL KILLER CELLS. They play an important role in regulating the cellular component of INNATE IMMUNITY.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
Glucose polymers consisting of a backbone of beta(1->3)-linked beta-D-glucopyranosyl units with beta(1->6) linked side chains of various lengths. They are a major component of the CELL WALL of organisms and of soluble DIETARY FIBER.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
Polysaccharides composed of repeating glucose units. They can consist of branched or unbranched chains in any linkages.
Proteins found in any species of fungus.
The functional hereditary units of FUNGI.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.

Impaired translesion synthesis in xeroderma pigmentosum variant extracts. (1/1728)

Xeroderma pigmentosum variant (XPV) cells are characterized by a cellular defect in the ability to synthesize intact daughter DNA strands on damaged templates. Molecular mechanisms that facilitate replication fork progression on damaged DNA in normal cells are not well defined. In this study, we used single-stranded plasmid molecules containing a single N-2-acetylaminofluorene (AAF) adduct to analyze translesion synthesis (TLS) catalyzed by extracts of either normal or XPV primary skin fibroblasts. In one of the substrates, the single AAF adduct was located at the 3' end of a run of three guanines that was previously shown to induce deletion of one G by a slippage mechanism. Primer extension reactions performed by normal cellular extracts from four different individuals produced the same distinct pattern of TLS, with over 80% of the products resulting from the elongation of a slipped intermediate and the remaining 20% resulting from a nonslipped intermediate. In contrast, with cellular extracts from five different XPV patients, the TLS reaction was strongly reduced, yielding only low amounts of TLS via the nonslipped intermediate. With our second substrate, in which the AAF adduct was located at the first G in the run, thus preventing slippage from occurring, we confirmed that normal extracts were able to perform TLS 10-fold more efficiently than XPV extracts. These data demonstrate unequivocally that the defect in XPV cells resides in translesion synthesis independently of the slippage process.  (+info)

Mutations of oncoprotein 18/stathmin identify tubulin-directed regulatory activities distinct from tubulin association. (2/1728)

Oncoprotein 18/stathmin (Op18) is a recently identified phosphorylation-responsive regulator of the microtubule (MT) system. It was originally proposed that Op18 specifically regulates dynamic properties of MTs by associating with tubulin, but it has subsequently been proposed that Op18 acts simply by sequestering of tubulin heterodimers. We have dissected the mechanistic action of Op18 by generation of two distinct classes of mutants. One class has interruptions of the heptad repeats of a potential coiled-coil region of Op18, and the other involves substitution at all four phosphorylation sites with negatively charged Glu residues. Both types of mutation result in Op18 proteins with a limited decrease in tubulin complex formation. However, the MT-destabilizing activities of the coiled-coil mutants are more severely reduced in transfected leukemia cells than those of the Glu-substituted Op18 derivative, providing evidence for tubulin-directed regulatory activities distinct from tubulin complex formation. Analysis of Op18-mediated regulation of tubulin GTPase activity and taxol-promoted tubulin polymerization showed that while wild-type and Glu-substituted Op18 derivatives are active, the coiled-coil mutants are essentially inactive. This suggests that Op18-tubulin contact involves structural motifs that deliver a signal of regulatory importance to the MT system.  (+info)

Regulation of human hsp90alpha gene expression. (3/1728)

Mammalian HSP90alpha and HSP90beta are encoded by two individual genes. On the basis of the upstream sequences of the human hsp90alpha gene, GenBank accession number U25822, we have constructed CAT reporter plasmids driven by individual fragments of the hsp90alpha gene. We found that (1) the proximal heat shock element complex located at -96/-60 enhances hsp90alpha promoter expression; (2) heat shock induction depends upon the coexistence of distal heat shock element at -1031/-1022 and the proximal heat shock element complex of the hsp90alpha gene; (3) unlike hsp90beta, downstream sequences of the transcription start site inhibit hsp90alpha expression. We conclude that the regulatory mechanisms for the expression of hsp90alpha and hsp90beta genes are different.  (+info)

unr, a cellular cytoplasmic RNA-binding protein with five cold-shock domains, is required for internal initiation of translation of human rhinovirus RNA. (4/1728)

Initiation of translation of the animal picornavirus RNAs occurs via a mechanism of direct ribosome entry, which requires a segment of the 5' UTR of the RNA, known as the internal ribosome entry site (IRES). In addition, translation of the enterovirus and rhinovirus (HRV) subgroups requires cellular trans-acting factors that are absent from, or limiting in rabbit reticulocytes, but are more abundant in HeLa cell extracts. It has been shown previously that HeLa cells contain two separable activities, each of which independently stimulates HRV IRES-dependent translation when used to supplement reticulocyte lysate; one of these activities was identified as polypyrimidine tract-binding protein (PTB). Here, the purification of the second activity is achieved by use of an RNA-affinity column based on the HRV 5' UTR. It comprises two components: a 38-kD protein (p38), which is a novel member of the GH-WD repeat protein family and has no intrinsic RNA-binding activity; and a 96- to 97-kD protein doublet, which was identified as unr, an RNA-binding protein with five cold-shock domains. Coimmunoprecipitation with antibodies against either protein shows that the two proteins interact with each other, and thus p38 is named unrip (unr-interacting protein). Recombinant unr acts synergistically with recombinant PTB to stimulate translation dependent on the rhinovirus IRES. In contrast, unr did not significantly augment the PTB-dependent stimulation of poliovirus IRES activity.  (+info)

Fertilization, embryonic development, and offspring from mouse eggs injected with round spermatids combined with Ca2+ oscillation-inducing sperm factor. (5/1728)

Round spermatids, precursor male gametes, are known to possess the potential to achieve fertilization and embryonic development when injected into eggs. However, injection of spermatids alone seldom activates eggs in the mouse, as spermatids by themselves cannot induce an increase in intracellular Ca2+, a prerequisite for egg activation. We injected a mouse round spermatid into an egg simultaneously with partially purified sperm factor from differentiated hamster spermatozoa. The combined injection produced repetitive Ca2+ increases (Ca2+ oscillations) lasting for at least 4 h as observed at fertilization, and induced activation in 92% of eggs. This method provided 75% fertilization success associated with male and female pronucleus formation and development to 2-cell embryos, while only 7% of eggs were fertilized by injection of a spermatid alone. Of the 2-cell embryos, approximately 50% developed to blastocysts during 5 days of culture in vitro, while no blastocysts were obtained following injection of sperm factor alone. Furthermore, the 2-cell embryos, that were created by spermatids and sperm factor and transplanted into foster mothers, developed into normal offspring, although the percentage was only 22%. All infants grew into healthy adults carrying normal chromosomes. The sperm factor served as a complementary factor for successful fertilization by round spermatid injection.  (+info)

Discrete regional distributions suggest diverse functional roles of calcium channel alpha1 subunits in sperm. (6/1728)

The Ca channels of male germ-line cells are partially characterized, but the molecular properties and subcellular localization of the Ca channels of mature sperm are unknown. Here, we probe rodent sperm with anti-peptide antibodies directed to cytosolic domains of cloned rat brain alpha1A, alpha1C, and alpha1E Ca channel subunits. Each recognizes a 200- to 245-kDa band on immunoblots of whole rat sperm extracts. A smaller ( approximately 110-kDa) alpha1C band also is detected. Confocal fluorescence images of mouse sperm show characteristic patterns of punctate alpha1A-, alpha1C-, and alpha1E-immunoreactivity. For alpha1A, the puncta are larger, less numerous, and more variable in distribution than for alpha1C and alpha1E. They are absent from the acrosomal crescent, but are present elsewhere over the sperm head, often at the apical tip and equatorial segment. They also are found at irregular intervals along both the midpiece and the principal piece of the flagellum. For alpha1C and alpha1E, puncta are dense along dorsal and ventral aspects of the acrosomal cap. For alpha1E but not alpha1C, the remainder of the acrosomal region also is labeled. Neither is found in the postacrosomal region or on the midpiece. Puncta of alpha1C and alpha1E occur at regular intervals each in two parallel rows, at the dorsal and ventral aspects of the proximal segment of the flagellar principal piece. The puncta in these arrays become less abundant and intense in the distal flagellum. These results demonstrate that multiple Ca channel proteins are present in mature sperm and are regionally localized in ways that may give them different regulatory roles.  (+info)

Defective repair of cisplatin-induced DNA damage caused by reduced XPA protein in testicular germ cell tumours. (7/1728)

Metastatic cancer in adults usually has a fatal outcome. In contrast, advanced testicular germ cell tumours are cured in over 80% of patients using cisplatin-based combination chemotherapy [1]. An understanding of why these cells are sensitive to chemotherapeutic drugs is likely to have implications for the treatment of other types of cancer. Earlier measurements indicate that testis tumour cells are hypersensitive to cisplatin and have a low capacity to remove cisplatin-induced DNA damage from the genome [2] [3]. We have investigated the nucleotide excision repair (NER) capacity of extracts from the well-defined 833K and GCT27 human testis tumour cell lines. Both had a reduced ability to carry out the incision steps of NER in comparison with extracts from known repair-proficient cells. Immunoblotting revealed that the testis tumour cells had normal amounts of most NER proteins, but low levels of the xeroderma pigmentosum group A protein (XPA) and the ERCC1-XPF endonuclease complex. Addition of XPA specifically conferred full NER capacity on the testis tumour extracts. These results show that a low XPA level in the testis tumour cell lines is sufficient to explain their poor ability to remove cisplatin adducts from DNA and might be a major reason for the high cisplatin sensitivity of testis tumours. Targeted inhibition of XPA could sensitise other types of cells and tumours to cisplatin and broaden the usefulness of this chemotherapeutic agent.  (+info)

Bcl-2 regulates a caspase-3/caspase-2 apoptotic cascade in cytosolic extracts. (8/1728)

Apoptosis is accompanied by the activation of a number of apoptotic proteases (caspases) which selectively cleave specific cellular substrates. Caspases themselves are zymogens which are activated by proteolysis. It is widely believed that 'initiator' caspases are recruited to and activated within apoptotic signalling complexes, and then cleave and activate downstream 'effector' caspases. While activation of the effector caspase, caspase-3, has indeed been observed as distal to activation of several different initiator caspases, evidence for a further downstream proteolytic cascade is limited. In particular, there is little evidence that cellular levels of caspase-3 that are activated via one pathway are sufficient to cleave and activate other initiator caspases. To address this issue, the ability of caspase-3, activated upon addition to cytosolic extracts of cytochrome c, to cause cleavage of caspase-2 was investigated. It was demonstrated that cleavage of caspase-2 follows, and is dependent upon, activation of caspase-3. Moreover, the activation of both caspases was inhibited by Bcl-2. Together, these data indicate that Bcl-2 can protect cells from apoptosis by acting at a point downstream from release of mitochondrial cytochrome c, thereby preventing a caspase-3 dependent proteolytic cascade.  (+info)

OCIAD1兔多克隆抗体(ab91574)可与人样本反应并经WB, ELISA, ICC/IF实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
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Clearsate.jpg CLEARSATE IS:A potent platelet lysate product to be used as a media supplement alternative to fetal bovine serum (FBS)Produced from expired human platelets and able to be used without an anticoagulantTriple-filter
Jun 1, 2017 , Platelet Lysate, Prolotherapy, PRP, Regenerative Treatments. June is National Scoliosis Awareness Month!. We can all agree that our spine is essential since it protects our spinal cord and also allows us to bend and move. However, for those with scoliosis, every day movements can become painful and debilitating. Scoliosis is a disease that affects about 2-3% of the population in the United States, roughly 6 to 9 million people. It usually has an early onset in children around 10-15 years old. The spine has a natural S curve when examined from a side view. It is when the S shape forms from side-to-side that problems can occur. This can be caused by factors such as: genetics, growth spurts in children, or bone degeneration. Symptoms vary by severity of the condition. For those with mild scoliosis (measured as less than 40 degrees), they might experience deformities such as having one shoulder higher than the other, or a slight hunchback form. People with curvatures greater than ...
PELOBIOTECH GmbH offers a broad range of primary cells, stem cells, genetically modified cell systems and 3D cell models. To be able to offer you these premium products we work together with companies worldwide. This will guarantee best quality and a huge number of highly innovative products and services ...
Discover our brand new portfolio of cell culture supplements! Our Human Platelet Lysate enables higher proliferation rates and a much better lot-to-lot consistency and availability than FBS.
TY - JOUR. T1 - Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton jelly mesenchymal stromal cells. AU - Chen, Ming Sheng. AU - Wang, Tsung Jen. AU - Lin, Hsiu Chen. AU - Thierry, Burnouf. PY - 2019/3/25. Y1 - 2019/3/25. N2 - There is accumulating experimental evidence that human platelet lysate (HPL) made from platelet concentrates can replace fetal bovine serum (FBS) as a xeno-free clinical-grade supplement of growth media to expand mesenchymal stromal cells (MSCs). However, uncertainties exist in regard to impacts that various manufacturing methods of HPL can exert on the expansion and differentiation capacity of MSCs. In particular, there is a need to evaluate the possibility of implementing virus-inactivation treatment during HPL production to ensure optimal safety of industrial HPL pools. Expired human platelet concentrates from four different donors were pooled and subjected to freeze-thaw cycles (-80/+37 °C), ...
Like OCIAD1, OCIAD2 is a cancer-related protein and its expression level increases during the course of malignant progression and is thought to be a very useful marker for evaluating the malignancy of ovarian mucinous tumors ...
MCF7 whole cell lysate Lysates datasheet (ab29537). Abcam offers quality products including antibodies, assays and other reagents.
Scaffolds for bone tissue engineering lack often control of cellular instructions. We propose a triple sequential approach for customizing scaffold features from the macro to the nanoscale. The nano/meso-scale is composed by human platelet lysate and marine-origin polysaccharides assembled by layer-by-layer and shaped into fibrils by freeze-drying. We show that osteogenic induction of stem cells is tunable within a low range of layers. This approach has the potential to develop new scaffolds with enhanced cell-instructive capabilities using affordable autologous sources of bioactive molecules ...
TY - CONF. T1 - Human platelet lysate gel (HPLG) supports and stimulates endothelial progenitor cell-driven vasculogenesis. AU - Pula, Giordano. AU - Fortunato, Tiago. AU - De Bank, Paul. N1 - Poster presentation at the XXV Congress of the International Society on Thrombosis and Haemostasis, 20-25 June 2015, Toronto, Canada.. PY - 2015/6. Y1 - 2015/6. M3 - Poster. T2 - XXV Congress of the International Society on Thrombosis and Haemostasis. Y2 - 20 June 2015 through 25 June 2015. ER - ...
Currently Unavailable Frog eggs: Rana genus. The actual species may vary depending on what is available at the time. We will ship as available in early spring. Per/clump. …
Background We recently developed and characterized a standardized and clinical grade human Platelet Lysate (hPL) that constitutes an advantageous substitute for fetal bovine serum (FBS) for human mesenchymal stem cell (hMSC) expansion required in cell therapy procedures, avoiding xenogenic risks (virological and immunological) and ethical issues. Because of the progressive use of pathogen-reduced (PR) labile blood components, and the requirement of ensuring the viral safety of raw materials for cell therapy products, we evaluated the impact of the novel procedure known as THERAFLEX UV-Platelets for pathogen reduction on hPL quality (growth factors content) and efficacy (as a medium supplement for hMSC expansion). This technology is based on short-wave ultraviolet light (UV-C) that induces non-reversible damages in DNA and RNA of pathogens while preserving protein structures and functions, and has the main advantage of not needing the addition of any photosensitizing additives (that might secondarily
Regenexx Platelet Lysate can offer relief from lower back or neck pain, buttock and leg pain, or numbness and tingling in the arms, hands, legs or feet. Its a safe, non-surgical treatment alternative for patients suffering from bulging discs and herniated cervical, thoracic, or lumbar disc related problems.. Most patients with bulging or herniated discs respond well to a platelet lysate injection into the space surrounding the inflamed spinal nerves. Other clinics resort to an injection of stems cells directly into a patients disc. While this can sometimes ultimately be necessary, our platelet lysate procedure is less invasive and lower risk. Nobody other than Regenexx offers this type of procedure, which utilizes natural growth factors produced from the patients own blood platelets. Regenexx is the only provider with experience in both stem cells and platelet lysate so we are uniquely able to determine the best treatment options to address your source of pain. ...
HeLa whole cell lysate Lysates datasheet (ab29545). Abcam offers quality products including antibodies, assays and other reagents.
ProSci offers more than 700 cell lysates that have been rigorously tested for your research projects. Order your research lysates online from ProSci today.
ProSci offers more than 700 cell lysates that have been rigorously tested for your research projects. Order your research lysates online from ProSci today.
Myc-DDK-tagged ORF clone of Homo sapiens OCIA domain containing 1 (OCIAD1), transcript variant 4 as transfection-ready DNA - 10 µg - OriGene - cdna clones
494; 1536; L895; S425; S424; 1048; T048; L030; 103; S175; S468; S832; S831; Q888; M365; CA19; 684; CA27; 698; Q620; 6050; M301; PCAN; X621; 130; 383; X587; 977; 1460; 0616; 0618; VAP1; ACT4; S602; C17; C12; C15; X860; T281; 1565; L067; L379; 2560; L529; T080; Q371; 433; 1717; 20; 21; NK57; CD57; 1065; 1532; CENP; 392; SMAC; CM24; 6520; 6513; 6510; S006; T261; T039; Q258; 135; S488; A870; 109; C1ES; X599; A125; Q469; S503; 468; V111; 4962; T449; M162; CDIF; 4964; T046; A867; S994; COC; 485; X605; X601; L251; 478; S240; 551; 0464; 0459; 666; 1978; Q240; 12; 2676; A937; T112; 950; S513; U204; S239; X328; S728; S726; T358; A322; A462; T228; 572; S867; M273; 110; CPKI; 712; S252; S120; 112; 497A; 2007; CRYO; Y224; A006; M371; N394; URCS; BFCG; 6011; EYCU; FGCO; FGCL; GENC; S491; Q602; NASC; SINC; S150; 7457; THAB; WOUC; L776; YEAS; YSTC; 2003; 7962; BLCU; EACU; STCU; A604; URCU; A900; 1074; 543; 5091; 5092; 5705; X624; CF60; X622; X613; Q190; X708; 619; 546; CYTI; NGYN; S410; S412; Q640; WODC; 306; L502;
FGF-2 and, to a lesser extent, PDGF-BB induced in adult human MSCs the expression of HLA-DR (normally induced by inflammatory cytokines), which was able to stimulate CD4+ T cells via superantigen binding. In contrast to IFNγ, FGF induced HLA-DR expression only in human MSCs proliferating under its mitogenic effect and not in mouse MSCs or in differentiated human cells. Although it induced cell proliferation, human platelet lysate did not cause HLA-DR expression in human MSCs. HLA-DR expression occurred following FGF-specific binding to its receptor(s), mainly FGF receptor 1, without inducing IFNγ or tumor necrosis factor α expression. Both MAPK/ERK-1/2 and phosphatidylinositol 3-kinase/Akt controlled cell proliferation and HLA-DR expression, but only MAPK/ERK-1/2 controlled the induction of the class II MHC transcription activator protein CIITA, the major determinant of HLA-DR transcription. ...
Purified Recombinant Human IL31RA cell lysate from Creative Biomart. Recombinant Human IL31RA cell lysate can be used for research.
Purified Recombinant Human PLIN2 293 Cell Lysate from Creative Biomart. Recombinant Human PLIN2 293 Cell Lysate can be used for research.
To engineer functional tissue substitutes, it is required a multi-component, multi-scale approach that combines both physical, chemical and biological cues. Fiber-based techniques have been explored in the field of tissue engineering to produce structures recapitulating tissue architecture and mechanical properties. In this work, we engineered biofunctional composite living fibers (CLF) as multi-compartment fibers with a mechanically competent core and a hydrogel layer. For this purpose, commercial silk suture threads were coated with a platelet lysate (PL) hydrogel by first embedding the threads in a thrombin solution and then incubating in PL. The fabrication set-up was optimized and the biological performance was studied by encapsulating human adipose-derived stem cells (hASCs). The developed coating process rendered CLF with a homogenous PL hydrogel layer covering suture threads. Encapsulated hASCs were viable up to 14 d in culture and were able to align at the surface of the core fiber and ...
TY - JOUR. T1 - Whats hot in the prostate?. AU - Gleave, Martin. AU - Evans, Christopher P.. PY - 2003. Y1 - 2003. UR - http://www.scopus.com/inward/record.url?scp=0347268981&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0347268981&partnerID=8YFLogxK. U2 - 10.1038/sj.pcan.4500699. DO - 10.1038/sj.pcan.4500699. M3 - Article. C2 - 14663465. AN - SCOPUS:0347268981. VL - 6. SP - 268. EP - 271. JO - Prostate Cancer and Prostatic Diseases. JF - Prostate Cancer and Prostatic Diseases. SN - 1365-7852. IS - 4. ER - ...
OK, well thanks to everyones advice we now have a clean and clear swimming pool. However, when we initially shocked the pool it was basically a mass murder on the local frog population.. We probably pulled out 4 or 500 dead tadpoles and close to 20 full grown frogs. We thought we had wiped them out, but we were wrong... Every morning I check the pool out and skim it and every morning I see all of these little black dots that are everywhere. At first I thought it was just dirt, but after
Adult stem cells are cells from your own body that can renew themselves and turn into other cells (differentiate). They live inside all of us in various tissues, poised to leap into action to repair damage as it occurs. As we age or have big injuries, we may not be able to recruit enough of these cells to the site to fully repair the area. Regenexx Stem Cell Procedures help overcome this problem by extracting stem cells from an area of high volume, then concentrating the cells and reinjecting them into the damaged area to help the body heal naturally. Our Patented Stem Cell Procedures can be used for a wide range of conditions and are the tool of choice for injuries, arthritis and other conditions that may be more significant than what may be treated with our Platelet Rich Plasma or Platelet Lysate Procedures. ...
Adult stem cells are cells from your own body that can renew themselves and turn into other cells (differentiate). They live inside all of us in various tissues, poised to leap into action to repair damage as it occurs. As we age or have big injuries, we may not be able to recruit enough of these cells to the site to fully repair the area. Regenexx Stem Cell Procedures help overcome this problem by extracting stem cells from an area of high volume, then concentrating the cells and reinjecting them into the damaged area to help the body heal naturally. Our Patented Stem Cell Procedures can be used for a wide range of conditions and are the tool of choice for injuries, arthritis and other conditions that may be more significant than what may be treated with our Platelet Rich Plasma or Platelet Lysate Procedures. ...
Adult stem cells are cells from your own body that can renew themselves and turn into other cells (differentiate). They live inside all of us in various tissues, poised to leap into action to repair damage as it occurs. As we age or have big injuries, we may not be able to recruit enough of these cells to the site to fully repair the area. Regenexx Stem Cell Procedures help overcome this problem by extracting stem cells from an area of high volume, then concentrating the cells and reinjecting them into the damaged area to help the body heal naturally. Our Patented Stem Cell Procedures can be used for a wide range of conditions and are the tool of choice for injuries, arthritis and other conditions that may be more significant than what may be treated with our Platelet Rich Plasma or Platelet Lysate Procedures. ...
Nuclear assembly is independent of linker histones.: The role of linker histones in the assembly of functional nuclei was examined with the use of a cell-free e
This image is part of a large data set of Xenopus laevis eggs imaged at various times post fertilization (the first number of the file name correspond...
This image is part of a large data set of Xenopus laevis eggs imaged at various times post fertilization (the first number of the file name correspond...
OM-85 treatment increases polyclonal antibody production with the capacity to neutralize influenza infection. BALB/c mice were treated with OM-85 or control sol
हम इन विट्रो में स्व-निरंतर mitotic दोलनों की पीढ़ी के लिए एक विधि वर्तमान में Xenopus laevis के अंडे के अर्क...
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Human reproductive cells. Some biologics that were previously extracted from animals, such as insulin, are now more commonly ... mammalian cell lines (see cell culture) and plant cell cultures (see plant tissue culture) and moss plants in bioreactors of ... somatic cells, gene therapies, tissues, recombinant therapeutic protein, and living cells used in cell therapy. Biologics can ... Extracted from living systems[edit]. Some of the oldest forms of biologics are extracted from the bodies of animals, and other ...
They are termed ENA because they can be extracted from the cell nucleus with saline.[7][14] The ENAs consist of ... T-cells and B-cells) and antigen presenting cells. These cells coordinate an immune response upon the detection of foreign ... the cell line was contaminated and displaced by HeLa cells, and has now been identified as actually HeLa cells.[54] ... Hargraves M, Richmond H, Morton R. Presentation of two bone marrow components, the tart cell and the LE cell. Mayo Clin Proc ...
Information Extracted. Limitations X-ray Diffraction(XRD)[11]. DAC or MAP. Powder or Single Crystal. cell parameters. ... cell parameters. Large Sample needed Infrared spectroscopy[12]. DAC. Powder, Single Crystal or Solution. Chemical Composition. ... Laser heating is done in a diamond-anvil cell with Nd:YAG or CO2 lasers to achieve temperatures above 6000k. Spectroscopy is ... The most common tool is a diamond anvil cell, which uses diamonds to put a small sample under pressure that can approach the ...
"Identification of a specific telomere terminal transferase activity in Tetrahymena extracts". Cell. 43 (2 Pt 1): 405-13. doi: ... Cell division is essential for an organism to grow, but, when a cell divides, it must replicate the DNA in its genome so that ... Within eukaryotic cells, DNA is organized into long structures called chromosomes. Before typical cell division, these ... and stop the DNA repair systems in the cell from treating them as damage to be corrected.[58] In human cells, telomeres are ...
The main chapters imported in 2012 were: coffee (75.3% of the total); juices and vegetable extracts (18.9%); nuts (3.5%); ... The main exported chapters consisted of: sounding devices (44.5% of the total); photovoltaic cells (20.4%); centrifugal pumps ( ...
The structure and properties of levan, a polymer of D-fructose produced by cultures and cell-free extracts of Aerobacter ... The structure and properties of levan, a polymer of D-fructose produced by cultures and cell-free extracts of Aerobacter ... 78:401-406, 1958 Xylosyl transfer by asparagus extracts. Feingold, D. S., neufeld, E. F. and Hassid, W. Z. J. Biol. Chem. 234: ... Interferon production in mice by cell wall mutants of Salmonella typhimurium. Youngner, J. S. and Feingold, D. S. Virology 1: ...
"Characterization of mediator complexes from HeLa cell nuclear extract". Molecular and Cellular Biology. 21 (14): 4604-13. doi: ... "Characterization of mediator complexes from HeLa cell nuclear extract". Molecular and Cellular Biology. 21 (14): 4604-13. doi: ... Cell nucleus. • nucleolus. Biological process. • regulation of transcription, DNA-templated. • phosphorylation. • protein ... Cell division protein kinase 8 is an enzyme that in humans is encoded by the CDK8 gene.[5][6] ...
Cell. 122 (3): 379-91. doi:10.1016/j.cell.2005.06.015. PMC 4401993. PMID 16096058. Glanzer J, Miyashiro KY, Sul JY, Barrett L, ... Jamison SF, Crow A, Garcia-Blanco MA (October 1992). "The spliceosome assembly pathway in mammalian extracts". Molecular and ... doi:10.1016/j.cell.2017.04.033. PMID 28502770. Schmucker D, Clemens JC, Shu H, Worby CA, Xiao J, Muda M, Dixon JE, Zipursky SL ... Zhang X, Yan C, Hang J, Finci LI, Lei J, Shi Y (May 2017). "An Atomic Structure of the Human Spliceosome". Cell. 169 (5): 918- ...
Kornberg T, Gefter ML (1971). "Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II". Proc. ... "Deoxyribonucleic acid synthesis in cell-free extracts. IV. Purification and catalytic properties of deoxyribonucleic acid ...
Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. „Cell". 43 (2 Pt 1), s. 405-13, ... Cell". 123 (6), s. 1121-31, Dec 2005. DOI: 10.1016/j.cell.2005.11.020. PMID: 16360040. ... Cell". 51 (6), s. 887-98, Dec 1987. PMID: 3319189. *EH. Blackburn, CW. Greider, E. Henderson, MS. Lee i inni. Recognition and ... Mol Cell". 24 (3), s. 423-32, Nov 2006. DOI: 10.1016/j.molcel.2006.10.020. PMID: 17070718. ...
"J. Cell Biol. 165 (1): 41-52. doi:10.1083/jcb.200309132. PMC 2172089 . PMID 15078901.. ... Terminally misfolded proteins, however, must be extracted from CNX/CRT. This is carried out by members of the EDEM (ER ... Cell Mol. Biol. 292: 197-280. doi:10.1016/B978-0-12-386033-0.00005-0. PMID 22078962.. ... In cells that are infected with HIV, SCFβTrCP interacts with Vpu and ubiquitinates CD4, which is subsequently degraded by the ...
Embryonic stem cells incorporate the altered gene, which replaces the already present functional copy. These stem cells are ... These segments can then be extracted through gel electrophoresis. If the chosen gene or the donor organism's genome has been ... As only a single cell is transformed with genetic material, the organism must be regenerated from that single cell. In plants ... DNA is generally inserted into animal cells using microinjection, where it can be injected through the cell's nuclear envelope ...
1 Preparation and properties of cell-free extracts". J. Biol. Chem. 240 (12): 4669-74. PMID 5846987. Bradbeer C (1965). "The ...
Sanguinarine kills animal cells by blocking the action of Na+/K+-ATPase transmembrane proteins. As a result, applying bloodroot ... Bloodroot and its extracts are thus considered escharotic. Although applying escharotic agents (including bloodroot) to the ... "Analysis by this department of a sample of the article showed that it consisted essentially of extracts of plant drugs ... Products made from sanguinaria extracts, such as black salve, are escharotic and can cause permanent damage. Claims that it can ...
"Methenyl-tetrahydromethanopterin cyclohydrolase in cell extracts of Methanobacterium". Arch. Biochem. Biophys. 242 (2): 430-9. ...
Philpott, Anna; Leno, Gregory H. (1992). "Nucleoplasmin remodels sperm chromatin in Xenopus egg extracts". Cell. 69 (5): 759- ... Dingwall, C.; Laskey, R. A. (1990). "Nucleoplasmin: the archetypal molecular chaperone". Seminars in Cell Biology. 1 (1).. ... Seminars in Cell Biology. 1 (1): 11-17. PMID 1983266. Rice, Philip; Garduno, Rafael; Itoh, Toru; Katagiri, Chiaki; Ausio, Juan ... Cell. 30 (2): 449-458. doi:10.1016/0092-8674(82)90242-2. PMID 6814762. Jun, SHUTe; Zhou, ZHANGYao (2007). "Nucleoplasmin, an ...
"Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c". Cell. 86 (1): 147-57. doi:10.1016 ... Cytochrome c also has an intermediate role in apoptosis, a controlled form of cell death used to kill cells in the process of ... Waterhouse NJ, Trapani JA (Jul 2003). "A new quantitative assay for cytochrome c release in apoptotic cells". Cell Death and ... Cytochrome c is known to play a role in the electron transport chain and cell apoptosis. However, a recent study has shown that ...
"Observation of the effect of Spirobolus bungii extract on cancer cells". Journal of Traditional Chinese Medicine. 1 (1): 34-8. ... The secretions of Spirobolus bungii have been observed to inhibit division of human cancer cells.[83] The only recorded usage ...
Younger cats tend to have T-cell lymphoma and older cats tend to have B-cell lymphoma. Older cats tend to have gastrointestinal ... Alternative treatments include vitamin C and Pau d'Arco (a bark extract). The prognosis for lymphoma in ferrets depends on ... Flow cytometry detects antibodies linked to tumour cell surface antigens in fluid samples or cell suspensions. Polymerase chain ... "Distinct B-cell and T-cell lymphoproliferative disease prevalence among dog breeds indicates heritable risk". Cancer Res. 65 ( ...
"Extracts of mosquito salivary gland inhibit tumour necrosis factor alpha release from mast cells". Parasite Immunology. 15 (1 ... 2005) observed a suppression of antibody-specific T cell responses mediated by mosquito saliva and dependent on mast cells and ... Cellular proliferation in response to IL-2 is clearly reduced by prior treatment of cells with mosquito salivary gland extract. ... T cell populations are decidedly susceptible to the suppressive effect of mosquito saliva, showing increased mortality and ...
Cell. Biochem. 5 (1-2): 55-63. doi:10.1007/BF01874172. PMID 4279328. Kalckar, H. M. Biological phosphorylations: development of ... Kalckar, H. M. (1939). "The Nature of Phosphoric Esters Formed in Kidney Extracts". Biochemical Journal. 33 (5): 631-641. doi: ... which they purified from muscle extracts. Further work on nucleotide metabolism allowed him to identify nucleoside ...
Hydrolysis of nucleic acids in crude cell-free extracts. Sequencing of RNA. Preparation of rabbit reticulocyte lysates. Studies ... A common source is E.coli cells carrying a cloned nuc gene encoding Staphylococcus aureus extracellular nuclease (micrococcal ...
Studies have shown that its extracts have renal effects. It has also been seen that in rats, such extracts cause an increase in ... The surface cells are loosely arranged. The number of chromosomes is 16, which is 2n = 8. It has been said that the species of ... "Effects of hydroalcoholic extracts of Portulaca pilosa and Achyrocline satureioides on urinary sodium and potassium excretion ...
"Toxic effects of Karenia mikimotoi extracts on mammalian cells". Chinese Journal of Oceanology and Limnology. 29 (4): 860-868. ...
One study showed that extracts of H. stigonocarpa suppressed cell division in onions, and this may imply that it contains some ... Fabaceae) on the cell cycle of Allium cepa L." Anais da Academia Brasileira de Ciências. 86 (3): 1147-1150. doi:10.1590/0001- ... Lacerda, Lourran P.; Malaquias, Geiz; Peron, Ana Paula (2014). "Antiproliferative action of aqueous extracts of Hymenaea ...
Knysh O, Martynov A (2020). "Lactobacillus reuteri cell-free extracts against antibiotic-resistant bacteria". Zaporozhye ... Cell. 165 (7): 1762-1775. doi:10.1016/j.cell.2016.06.001. PMC 5102250. PMID 27315483. Wang XD, Soltesz V, Molin G, Andersson R ...
Yeast extract is made by centrifuging inactive nutritional yeast and concentrating the water soluble yeast cell proteins which ... Nutritional yeast is a whole cell inactive yeast that contains both soluble and insoluble parts; which is different from yeast ... Microbial Cell Factories. 16 (1): 15. doi:10.1186/s12934-017-0631-y. ISSN 1475-2859. PMC 5282855. PMID 28137297. "Nutritional ...
Fermentation is another process by which cells can extract energy from glucose. It is not a form of cellular respiration, but ... This phenomenon is called the Warburg effect and is found primarily in cancer cells. Muscles cells under great exertion will ... Generally, lactic acid fermentation occurs only when aerobic cells are lacking oxygen. However, some aerobic mammalian cells ... Molecular Cell Biology (7th ed.). W. H. Freeman and Company. pp. 520-523. ISBN 978-1-4292-3413-9. Voet, Donald & Voet, Judith G ...
... is typically extracted by ethanol precipitation from liquid culture or by alkaline treatment from the cell wall. Once extracted ... Cell Microbiol 12, 405-410 Robinet, P., Baychelier, F., Fontaine, T., Picard, C., Debre, P., Vieillard, V., Latge, J.P., and ... Bardalaye, P.C., and Nordin, J.H. (1976). Galactosaminogalactan from cell walls of Aspergillus niger. J Bacteriol 125, 655-669 ... Similar to other fungal cell wall polysaccharides, galactosaminogalactan is synthesized by polymerization of nucleotide sugars ...
An extract of the plant's fruit Fructus psoraleæ has been shown to act as a norepinephrine-dopamine reuptake inhibitor in vitro ... inhibits mitochondrial complex I and proteasome activities in SH-SY5Y cells". Annals of the New York Academy of Sciences. 1100 ... Extracts obtained from the seeds of P. corylifolia have been shown to inhibit mitochondrial complex I in vitro and may ... Zhao G, Li S, Qin GW, Fei J, Guo LH (2007). "Inhibitive effects of Fructus Psoraleae extract on dopamine transporter and ...
6,258,540, which claims methods of using cell-free fetal DNA (cffDNA) circulating in maternal plasma (cell-free blood) to ... "The claimed method is a new process of detecting cffDNA by devising a novel sample source from which to extract it, namely, ...
Certain cells in the brain respond specifically to an increase of CO2 in the blood.[4][24] The response involves vasodilatation ... by extracting a piece of bone.[42] Any correction of the nasal tip, which points towards the contralateral side, will not be ... Symmetry is restored by extracting a small piece of bone from the supraorbital bar at the contralateral side, thereby reducing ... Parts of the flattened occiput are extracted and given a rounder shape by greenstick fracturing them.[11] ...
Hamming codes are only suitable for more reliable single-level cell (SLC) NAND. Denser multi-level cell (MLC) NAND requires ... the corruption of some symbols by noise usually allows the original user data to be extracted from the other, uncorrupted ... "Error Correction Code in Single Level Cell NAND Flash memories" 16 February 2007 ...
In vitro modulation of oxidative burst via release of reactive oxygen species from immune cells by extracts of selected ... Gurib-Fakim, A.; Sewraj, M.; Gueho, J.; Dulloo, E.. «Studies on the Antisickling Properties of Extracts of Sideroxylon ... Mahomoodally, M. F.; Gurib-Fakim, A.; Subratty, A. H.. «Momordica charantia extracts inhibit uptake of monosaccharide and amino ... MAHOMOODALLY, M.; GURIB FAKIM, A.; SUBRATTY, A. H.. «INHIBITORY EFFECTS OF A TRADITIONAL ANTIDIABETIC MEDICINAL FRUIT EXTRACT ...
Cette raison a engagé M. Mosander à donner au nouveau métal le nom de Lantane." (The oxide of cerium, extracted from cerite by ... The element is known to damage cell membranes of water animals, causing several negative influences on reproduction and on the ... He separated it from pitchblende residues left by Marie and Pierre Curie after they had extracted radium. In 1899, Debierne ... Lanthanum is much more common, being the second most abundant rare earth, and in addition to monazite can also be extracted ...
Cellular respiration, the process in which nutrients are converted into useful energy in a cell *Anaerobic respiration, ... Aquatic respiration, animals extracting oxygen from water. *Artificial respiration, the act of simulating respiration, which ... Respiration (physiology), transporting oxygen and carbon dioxide between cells and the external environment *Respiratory system ...
... has been known to stimulate cell growth in normal and cancer cell line cultures,[37] and it was shown that ... The original discovery of Substance P (SP) was in 1931 by Ulf von Euler and John H. Gaddum as a tissue extract that caused ... on cells (including cancer cells) bestowing upon them mobility.[40] and metastasis.[41] It has been suggested that cancer ... stem cells, white blood cells) in many tissues and organs. SP amplifies or excites most cellular processes.[15][16] ...
It is also possible to extract stem cells from amniotic fluid for both autologous or heterologous use at the time of childbirth ... who have lost their stem cells after birth. Other conditions[13] treated with stem cell transplants include sickle-cell disease ... Peripheral blood stem cells[26] are now the most common source of stem cells for HSCT. They are collected from the blood ... Sources and storage of cells[edit]. To limit the risks of transplanted stem cell rejection or of severe graft-versus-host ...
If you extract that energy, they will slow down like anything else would. --Jayron32 11:59, 2 August 2021 (UTC). Would a oloid ... doi:10.1016/j.cell.2021.06.02: Convalescent serum (i.e. antibodies in blood from people previously infected) of people who had ...
... the yeast cells appear globose, ellipsoidal or cylindrical, 2-6 x 3-11 μm in size.[6] In a glucose-yeast extract broth, K. ... The formation of the ascospores occurs through the conjugation of the haploid cells preceding the formation of the ascus.[8] ... Alternatively, ascosporogensis can arise directly from diploid cells.[8] Each ascus contains 1-4 ascospores.[8] The ploidy of K ...
Thyroxine was first isolated in pure form in 1914 at the Mayo Clinic by Edward Calvin Kendall from extracts of hog thyroid ... T4 and T3 bind to thyroid receptor proteins in the cell nucleus and cause metabolic effects through the control of DNA ...
"The Resurrection of Cell and Frieza" / "The Villains Of Hell!! The Revival of Cell and Frieza". Transcription: "Jigoku no ... Baby extracts himself from this person, revealing that it was he who was possessing these mysterious fighters. During his ... Cell and Frieza show up, and now that he's trapped in hell, he has no choice but to fight them. ... He says that there is a single cell on the chest of the robot, and that hitting it with simultaneous blasts from the inside and ...
At the same time, Michael confronts Leila over the call made using the stolen cell phone. He then kills Leila's handler as he ... In Murmansk, a scientist is extracting the weapon from a frozen body when he accidentally cuts himself and infects himself with ... At the end, Leila manages to contact Sean using a stolen cell phone and informs him of Sophia's plan. ...
Even up to the present day, a flask of manna is extracted from the tomb of Saint Nicholas every year on 6 December (the Saint's ... but Christ and the Virgin Mary appear to him in his cell.[48][54] He tells them he is imprisoned "for loving you"[48] and they ...
The mesoderm-derived epithelial cells of the sex cords in developing testes become the Sertoli cells, which will function to ... These are Leydig cells. Soon after they differentiate, Leydig cells begin to produce androgens. ... Dihydrotestosterone increased the number of BrdU cells, while flutamide inhibited these cells. ... Cell Biol. 42 (6): 813-27. doi:10.1016/j.biocel.2009.11.013. PMID 19931639.. ...
Potassium is the major cation (positive ion) inside animal cells,[223] while sodium is the major cation outside animal cells.[ ... Lithium salts have to be extracted from the water of mineral springs, brine pools, and brine deposits. The metal is produced ... The balance between potassium and sodium is maintained by ion transporter proteins in the cell membrane.[231] The cell membrane ... Unit cell ball-and-stick model of lithium nitride.[118] On the basis of size a tetrahedral structure would be expected, but ...
A combination of bile and bilirubin, which comes from dead red blood cells, gives feces the typical brown color.[1][2] ... To extract and analyze the information contained within, researchers generally have to freeze the feces and grind it up into ... and the body starts expelling bilirubin from dead red blood cells, its matter acquires the familiar brown color.[2] ... and the dead epithelial cells from the lining of the gut.[1] ...
Cell Biol. 42 (6): 813-27. doi:10.1016/j.biocel.2009.11.013. PMID 19931639.. ... and saw palmetto extract (Serenoa repens).[63] A number of other antiandrogens, including cyproterone acetate, spironolactone, ... Bennett NC, Gardiner RA, Hooper JD, Johnson DW, Gobe GC (2010). "Molecular cell biology of androgen receptor signalling". Int. ... cells in culture". Journal of Steroid Biochemistry. 31 (5): 845-52. doi:10.1016/0022-4731(88)90295-6. PMID 2462135.. ...
... to higher temperatures and either combusted to do work or in the case of hydrogen gas extracted for use in a hydrogen fuel cell ... Exner, T; Ahuja, M; Ellwood, L (24 April 2019). "Effect of an activated charcoal product (DOAC Stop™) intended for extracting ... Activated carbon is useful for extracting the direct oral anticoagulants (DOACs) such as dabigatran, apixaban, rivaroxaban and ...
Blood is then extracted; the serum is separated, purified and freeze-dried.[146] The cytotoxic effect of snake venom is being ... Cell. 25 (4): 326-328. doi:10.1016/j.devcel.2013.05.011. PMID 23725759.. ...
Cells, circulating tumor cells (CTCs), or formalin-fixed paraffin-embedded (FFPE) or frozen tissue sections are fixed, then ... The artificial chromosomes (BAC) can be grown, extracted, and labeled, in any lab containing a library. Genomic libraries are ... FISH can also be used to detect diseased cells more easily than standard Cytogenetic methods, which require dividing cells and ... "Defining the sister rat mammary tumor cell lines HH-16 cl.2/1 and HH-16.cl.4 as an in vitro cell model for Erbb2". PLOS ONE. 7 ...
The viruses infect, amongst others, monocytes, macrophages, and dendritic cells. They attach to the cell surfaces via specific ... A French team developed the French neurotropic vaccine (FNV), which was extracted from mouse brain tissue. Since this vaccine ... After entering the host cell, the viral genome is replicated in the rough endoplasmic reticulum (ER) and in the so-called ... After transmission from a mosquito, the viruses replicate in the lymph nodes and infect dendritic cells in particular. From ...
A stained preparation of the cell Bacillus subtilis showing endospores as green and the vegetative cell as red ... The spores germinated when the amber was cracked open and the material from the gut of the bee was extracted and placed in ... Endospores are resistant to most agents that would normally kill the vegetative cells they formed from. Unlike persister cells ... The DNA is replicated and a membrane wall known as a spore septum begins to form between it and the rest of the cell. The ...
Thus, the extracting and purifying methods used to extract iron in a blast furnace can affect the quality of steel that is ... Lighter and cheaper wires, antistatic materials, organic solar cells Femtotechnology, picotechnology Hypothetical Present ... Other examples can be seen in the "plastic" casings of television sets, cell-phones and so on. These plastic casings are ... Besides material characterization, the material scientist or engineer also deals with extracting materials and converting them ...
"Cell. 157 (6): 1380-1392. doi:10.1016/j.cell.2014.05.009. PMC 4144415 . PMID 24906154.. ... Boron is an essential plant nutrient, required primarily for maintaining the integrity of cell walls.[45][46][47] Boron has ... A component of bones (see apatite), cells, in energy processing, in DNA and ATP (as phosphate) and many other functions Red ... Needed for muscle, heart and digestive system health, builds bone, supports synthesis and function of blood cells Dairy ...
Using a β2 adrenergic receptor preparation derived from transfected HEK 293 cells, Liappakis and co-workers found that in wild- ... and Halostachys caspica extracts have been included as a constituent of certain OTC dietary supplements, but halostachine has ... Measurements of cAMP accumulation in intact transfected HEK 293 cells, after treatment with EEDQ to inactivate 98-99% of the ...
Bark extracts of Lannea coromandelica caused lysis of cell membranes followed by fragmentation of cellular materials. ...
Further information: Cell wall § Archaeal cell walls. Most archaea (but not Thermoplasma and Ferroplasma) possess a cell wall.[ ... Viscosity increase in soluble extracts". BioSystems. 29 (2-3): 151-60. doi:10.1016/0303-2647(93)90091-P. PMID 8374067.. ... Cell division is controlled in a cell cycle; after the cell's chromosome is replicated and the two daughter chromosomes ... In euryarchaea the cell division protein FtsZ, which forms a contracting ring around the cell, and the components of the septum ...
In yeast cells and isolated mitochondria, α-pinene and β-pinene, plant extract of "Melaleuca alternifolia" destroy cellular ... Timorex Gold is a natural botanical broad spectrum fungicide with prophylactic and curative activity, based on a plant extract ... The unique and powerful potential of this plant extract is highly effective for control in agricultural crops while ...
Konradt C, Ueno N, Christian DA, Delong JH, et al «Endothelial cells are a replicative niche for entry of Toxoplasma gondii to ... Drinić M, Wagner A, Sarate P, Zwicker C, et al «Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against ...
... Satoshi Naito naito at abs.agr.hokudai.ac.jp Wed Nov 11 19:13:37 EST 1998 *Previous message: ... Dear Netters, Is there anyone who assayed GFP fluorescence in plant/cell extracts ...
... or contents of a cell, as well as the cells protection status and the filename. Use the INFO function to return information ... Use the CELL function to return information about the formatting, location, ... Use the CELL function to return information about the formatting, location, or contents of a cell, as well as the cells ... in cell C1,…Im gonna type equal cell,…left parentheses,…double quote,…file name.… ...
... shows this synthesized compound induces the cell death known as apoptosis in prostate cancer cells while leaving healthy cells ... "Weve shown similar anti-cancer activity in the past with grape seed extract (GSE), but now we know B2G2 is its most ... For example, previous studies have shown the GSE effectiveness against cancer cells and have also shown its mechanism of action ... "its expensive and it takes a long time to isolate B2G2 from grape seed extract," Tyagi says. ...
This step is necessary to equalize the amount of cells (and protein) in a given volume of whole cell extract. ... 5. Calculate the volume of resuspended cells that would translate to an OD600 nm reading of 10. For example, this would equal 1 ... 6. Transfer the calculated volume of resuspended cells to a microfuge tube and centrifuge at 16,000 x g for 1 min. ... Home / Resources / Laboratory Methods / SDS Whole Cell Extracts. SDS Whole Cell Extracts ...
Join Dennis Taylor for an in-depth discussion in this video Extracting information with the CELL and INFO functions, part of ... Join Dennis Taylor for an in-depth discussion in this video Extracting information with the CELL and INFO functions, part of ... The two that were going to look at in this movie are Cell and Info. …Both of them give us information about the worksheet, the ... Just imagine that we might want to …display the current file name and its location, equal cell left parenthesis and …look at ...
... which limits cells to a fixed number of divisions. During cell replication, the telomeres function by ensuring the cells ... "Withania somnifera Root Extract Enhances Telomerase Activity in the Human HeLa Cell Line"​. Authors: V. Raguraman, J.R. ... RCT: Ashwagandha root extract may improve memory and cognitive functions * RCT supports Ashwagandha root extracts weight ... Ashwagandha root extracts shows anti-aging effect: Cell study. By Stephen Daniells ...
We examined the effects of algae extracts on normal hematopoietic cells and leukemia cells. Ethanol extracts were prepared of ... Cell-cycle analysis of AML cell lines showed G0/G1 arrest in the presence of AFA. These data suggest that algae extracts may ... Cytotoxicity of Algae Extracts on Normal and Malignant Cells. Jeremy Bechelli,1 Myra Coppage,2 Karen Rosell,1 and Jane Liesveld ... High AFA concentrations decreased viability of normal marrow cells. Normal C. D. 3. 4. +. viability was inhibited by Dun. Dun ...
Activity of a Chick Collagen Gene in Heterologous and Homologous Cell-Free Extracts. ... Activity of a Chick Collagen Gene in Heterologous and Homologous Cell-Free Extracts. In: Kumar A. (eds) Eukaryotic Gene ... Hansen, U., Tenen, D. G., Livington, D. M., and Sharp, P. A., 1981, Cell 27: 603-612.PubMedCrossRefGoogle Scholar ... Weil, P. A., Luse, D. S., Segall, J., and Roeder, R. G., 1979, Cell 18: 469-484.PubMedCrossRefGoogle Scholar ...
Nissan is testing a different kind of fuel-cell electric car that extracts hydrogen onboard from biofuel, such as ethanol. Read ... Nissans Fuel-Cell Car Hits the Road in Brazil, Extracts Hydrogen from Biofuel. ... Hydrogen Stations Needed for Fuel-Cell Vehicles of the Future * Toyota Details 2015 Hydrogen Fuel-Cell Vehicle: 300-Plus-Miles ... Unlike the fuel cells used by the Honda Clarity, the Toyota Mirai, and the Hyundai Tucson, which convert hydrogen and air to ...
Find the most comprehensive real-world treatment information on Animal Embryo Cell Extracts at PatientsLikeMe. 0 patients with ... bipolar I disorder or psoriasis currently use Animal Embryo Cell Extracts. ... What is Animal Embryo Cell Extracts?. Category: Others false Animal Embryonic Live Cell Therapy uses liquid extracts obtained ... Stopped using Animal Embryo Cell Extracts Duration. Patients. This item is relevant to you: 1 - 6 months 1 * 1 ...
if not impossible for any single Formula or VBA Macro to extract the data.. If it is only a single long string of text. Where ... All of these are in one column; each has its separate cell, but not all of the companies has a website listing - does this make ... I am trying to extract email addresses only from one column that contains various types of contact data, including email ...
... has been backed by a new study finding that it can selectively target cancer cells. ... and cancer-targeting ability of the broccoli extract sulforaphane, ... cell cycle arrest and apoptosis in normal prostate cells versus hyperplastic and cancerous prostate cells"​. Authors: J.D. ... "cell death in cancer cell lines, but not in healthy cell tissue. ... Broccoli extract can target cancer cells: Study. By Nathan ...
Phosphorylated SAPK/JNK Control Cell Extracts: Total cell extracts from 293 cells, treated with 50 mJ UV light and a 30 minute ... Nonphosphorylated SAPK/JNK Control Cell Extracts: Total cell extracts from 293 cells, serve as a negative control. Supplied in ... SAPK/JNK Control Cell Extracts 9253. Toggle Between Dark and Light Modes Filter: *WB ... Western blot analysis of extracts from 293 cells, treated with or without UV light, using Phospho-SAPK/JNK (Thr183/Tyr185) ( ...
... extract. In contrast, the leaf but not root extract inhibited the enzymatic activity of cyclooxygenase-2. While both extracts ... We focused on defining the effects of Echinacea purpurea extracts in dendritic cells (DCs), which generate innate and adaptive ... The fate and function of DCs from C57Bl/6 mice was evaluated following 48h exposure to E. purpurea root and leaf extracts. Flow ... We hypothesized that E. purpurea extracts would enhance murine bone marrow-derived DC (BMDC) activation leading to increased ...
An experimental process that snags lung cancer cells from a blood sample could give doctors real-time feedback on the most ... Cancer cells extracted from blood. An experimental process that snags lung cancer cells from a blood sample could give doctors ... In December, the same group reported in Nature that their circulating tumour cells, or CTC, chip could extract malignant cells ... and Harvard Medical School and colleagues were able to extract blood-borne cancer cells from 27 volunteers with non-small-cell ...
Role for regulated phosphatase activity in generating mitotic oscillations in Xenopus cell-free extracts.. Zhang T1, Tyson JJ, ... Role for regulated phosphatase activity in generating mitotic oscillations in Xenopus cell-free extracts ... Role for regulated phosphatase activity in generating mitotic oscillations in Xenopus cell-free extracts ... Role for regulated phosphatase activity in generating mitotic oscillations in Xenopus cell-free extracts ...
"Root bark extracts of Juncus effusus and Paeonia suffruticosa protect salivary gland acinar cells from apoptotic cell death ... Methanol and Butanol Extracts of Paeonia lutea Leaves Repress Metastasis of Squamous Cell Carcinoma. Yoshiki Mukudai,1 Meilin ... "Aqueous extract of Paeonia suffruticosa inhibits migration and metastasis of renal cell carcinoma cells via suppressing VEGFR-3 ... D. Brenner and T. W. Mak, "Mitochondrial cell death effectors," Current Opinion in Cell Biology, vol. 21, no. 6, pp. 871-877, ...
... can be rendered soluble and delivered to tumours to kill cancer cells, scientists say. ... Extracts of turmeric - a condiment commonly used in Indian cuisine - ... Extracts of turmeric - a condiment commonly used in Indian cuisine - can be rendered soluble and delivered to tumours to kill ... Pans team also hopes to prove that this method will be effective in killing cancer stem cells, in effect cancers root system ...
In December, the same group reported in Nature that their circulating tumor cells, or CTC, chip could extract malignant cells ... and Harvard Medical School and colleagues were able to extract blood-borne cancer cells from 27 volunteers with non-small-cell ... said there are practical questions about whether enough cells can be extracted to make the technique effective and whether it ... Food and Drug Administration-approved technique that uses magnetic beads to try to extract cancer cells, according to Haber. ...
"In an oncology setting, fucoidan [seaweed extract] acts via a number of cell pathways leading to cell cycle arrest in the first ... showed two compounds extracted from seaweed were found to halt the cell cycle, and prevent cancer cells from dividing and ... Seaweed extracts could inhibit cancer cell growth, says Australian biotech By Flora Southey ... Seaweed extracts can reduce tumour growth and improve the effectiveness of chemotherapy says Marinova, which is developing the ...
Cell cycle-dependent phosphorylation of smooth muscle myosin light chain in sea urchin egg extracts.. Mishima M1, Mabuchi I. ... The activity in the presence of EGTA showed cell cycle-dependent changes similar to that of histone H1 kinase, namely, it ... We studied enzymatic activities in sea urchin egg extracts that phosphorylate myosin regulatory light chain (MRLC) from chicken ...
The major products of colicin E1 plasmid DNA synthesis in cell extracts are completely replicated molecules and a class of ... Replication of Colicin E1 Plasmid DNA in Cell Extracts: II. Selective Synthesis of Early Replicative Intermediates. Yoshimasa ... Replication of Colicin E1 Plasmid DNA in Cell Extracts: II. Selective Synthesis of Early Replicative Intermediates ... Replication of Colicin E1 Plasmid DNA in Cell Extracts: II. Selective Synthesis of Early Replicative Intermediates ...
Brown University scientists were able to retrieve a high yield of cells that showed an especially strong propensity to make ... By sorting human fat tissue cells by their expression of a certain gene, ... sorting cells based on surface proteins presumed to indicate that a cell is a stem cell (4 percent). ... A new way to extract bone-making cells from fat tissue. Brown University ...
Active Agents »B2G2 »Colorado river »GSE »Pharmacy »anti-cancer activity »cancer cells »grape seed extract » gWe fve ... Solar cells convert sunlight into electricity. During this process, the electrons of the material inside the cell absorb the ... Active component of grape seed extract effective against cancer cells. 05.12.2013 ... An active ingredient of grape seed extract, B2G2, acts against cancer cells. ...
PHILADELPHIA - Bitter melon extract, a common dietary supplement, exerts a significant effect against breast cancer cell growth ... Bitter melon extract is cultivated in Asia, Africa and South America. Extract of this vegetable is being popularized as a ... "Our findings suggest that bitter melon extract modulates several signal transduction pathways, which induces breast cancer cell ... Using human breast cancer cells and primary human mammary epithelial cells in vitro, Ray and colleagues found the mechanism of ...
Cell-free extracts from Saccharomyces cerevisiae catalyzed the incorporation of glucosyl residues from UDP-[U-14C]glucose into ... Cell-free extracts from Saccharomyces cerevisiae catalyzed the incorporation of glucosyl residues from UDP-[U-14C]glucose into ... 22355 - Biosynthesis of beta-glucans by cell-free extracts from saccharomyces cerevisiae.. 391565 - A novel atp-driven glucose ... Cell Cycle. Cell-Free System. Glucosyltransferases / metabolism. Glycogen Synthase / metabolism. Hydrogen-Ion Concentration. ...
NF Cell Proliferation Assay that is based on the estimation of DNA content in viable cells. Cell viability decreased with ... In the search for a cell proliferation assay that would not be directly affected by the plant extracts, we identified the ... Intrinsic reductive potential was observed in a cell-free system when extracts were added directly to the CellTiter assay ... Conflicting results were obtained from this assay whereby higher concentrations of extracts significantly increased cell ...
Tomato extracts KILL stomach cancer cells, new study shows. Wednesday, June 13, 2018 by: Isabelle Z. Tags: antioxidants, cancer ... could stop malignant stomach cancer cells from cloning and growing. The whole tomato extracts stopped the cells from migrating ... www.naturalnews.com/2018-06-13-tomato-extracts-kill-stomach-cancer-cells-new-study-shows.html",Tomato extracts KILL stomach ... The researchers analyzed the lipophilic extracts of whole tomatoes to determine their impact on the neoplastic features of cell ...
Galectin-3 is also said to help malignant cells escape apoptosis, or programmed cell death. In turn, these cancerous cells can ... Natural citrus fruit extract can activate cancer-killing cells. Saturday, January 14, 2017 by: Vicki Batts Tags: anticancer ... www.naturalnews.com/2017-01-14-natural-citrus-fruit-extract-can-activate-cancer-killing-cells.html",Natural citrus fruit ... a study published in 2015 by the journal PLOS One found that MCP induced cell death in two different types of cancer cells. ...
In this study we show the biological effect of Echinacea augustifolia extract on cell viability and cell differentiation in ... Effect of Echinacea augustifolia extract on cell viability and differentiation in mammary epithelial cells. ... These effects have been observed in two different cell line derived from mouse (HC11) and bovine (BME-UV). Echinacea extract ... enhanced cell liability from 100 to 1000 ng/ml in association with growth factors, epidermal growth factor (EGF) or insulin, ...
  • A University of Colorado Cancer Center study published online ahead of print in the journal Nutrition and Cancer describes the laboratory synthesis of the most active component of grape seed extract, B2G2, and shows this synthesized compound induces the cell death known as apoptosis in prostate cancer cells while leaving healthy cells unharmed. (redorbit.com)
  • Primary CLL cells showed apoptosis at 24 hours after exposure to Dun, Ast, Spir, and AFA. (hindawi.com)
  • Galectin-3 is also said to help malignant cells escape apoptosis, or programmed cell death. (naturalnews.com)
  • A research group of the University of Granada has found out that maslinic acid, a compound present in the leaf and the olive skin wax extracted from alpeorujo (crushed olive pulp), has the capacity of preventing cancer as well as regulating apoptosis in carcinogenic processes. (eurekalert.org)
  • These findings indicated that thymoquinone induced cell death in oral cancer cells via two distinct anti-neoplastic activities that can induce apoptosis and autophagy. (greenmedinfo.com)
  • Using a commercially available grape seed extract, Shi exposed leukemia cells to the extract in different doses and found the marked effect in causing apoptosis in these cells at one of the higher doses.Given that epidemiological evidence shows that eating vegetables and fruits helps prevent cancer development, Shi and his colleagues have been studying chemicals known as proanthocyanidins in fruits that contribute to this effect. (goodnewsnetwork.org)
  • Shi has found that apple peel extract contains these flavonoids, which have antioxidant activity, and which cause apoptosis in several cancer cell lines but not in normal cells. (goodnewsnetwork.org)
  • The investigators, who report their findings in the January 1, 2009, issue of Clinical Cancer Research , a journal of the American Association for Cancer Research, also teased apart the cell signaling pathway associated with use of grape seed extract that led to cell death, or apoptosis. (goodnewsnetwork.org)
  • The researchers then used pharmacologic and genetic approaches to determine how the extract induced apoptosis. (goodnewsnetwork.org)
  • In their own laboratory study, Kane and Sadava found that the extract encouraged natural cell death, also known as apoptosis. (cityofhope.org)
  • In the cell model, the Noni leaf extract "inhibited the proliferation and induced apoptosis" (programmed cell death). (davidwolfe.com)
  • We previously identified that vanillin, an active constituent of vanilla, enhances TRAIL-induced apoptosis in cancer cells via suppression of NF-κB ( 9 ). (spandidos-publications.com)
  • The study revealed that this compound can cause cell death termed as "apoptosis" in prostate cancer cells and does not disturb the healthy cells in the body. (themedguru.com)
  • Further analysis revealed that cell death induced by this extract was associated with intracellular calcium release, opening of the MPTP, caspase 3- and PARP-cleavage suggesting that this extract induced aberrant calcium signaling that resulted in apoptosis via the mitochondrial pathway. (spandidos-publications.com)
  • The results from the current study suggest that the dichloromethane extract derived from Scrophularia orientalis effectively kills NB cells by inducing apoptosis via calcium release and consequently, the opening of mPTP. (spandidos-publications.com)
  • This study investigates the effect of eosinophil MBP extract on the expression of oncogenes p53, bcl-xl, bax, and c-myc, which modulate tumor growth, proliferation, and apoptosis. (scirp.org)
  • Treated prostate tumor cell lines trended toward apoptosis-induction, as evident in bcl-xl/bax ratios (scirp.org)
  • Dandelion root extract causes cancer cells to go through apoptosis. (davidwolfe.com)
  • Apoptosis is a natural cell process where a cell will activate an intracellular death program because it is no longer needed. (davidwolfe.com)
  • Two cells perform apoptosis or cell suicide. (davidwolfe.com)
  • The investigators found that grape seed extract selectively induced apoptosis (programmed cell death) in human colorectal cancer cells. (oncologynurseadvisor.com)
  • Thus the present study revealed that the GA isolated from the pomegranate peel extract (Kabul variety) induced apoptosis in A549 cells through intrinsic pathway. (scirp.org)
  • S. Elango, R. Balwas and V. Padma, "Gallic Acid Isolated from Pomegranate Peel Extract Induces Reactive Oxygen Species Mediated Apoptosis in A549 Cell Line," Journal of Cancer Therapy , Vol. 2 No. 5, 2011, pp. 638-645. (scirp.org)
  • Sodium iodate induces oxidative stress, photoreceptor cell disorganization and apoptosis through RPE disintegration and dysfunction. (arvojournals.org)
  • PA1 caused apoptosis in several cancer-cell lines in culture. (scielo.cl)
  • Apoptosis as the mechanism of cell death was confirmed by morphology study, caspases activity assay, as well as apoptosis related gene expression, Bcl-2 . (frontiersin.org)
  • In HeLa cells, the activity of caspase-9 and -3 and downregulation of Bcl-2 shows intrinsic pathway or mitochondrial pathway, whereas HepG2 shows caspase independent apoptosis. (frontiersin.org)
  • The loss of balance between cell proliferation and apoptosis is a hallmark that increases the failure of damaged cells to be eliminated through apoptosis. (frontiersin.org)
  • Fluorescence microscopy revealed characteristic morphological features of apoptosis and detected the appearance of apoptotic bodies following treatment with the extract in the PC cells. (unboundmedicine.com)
  • Researchers found that sea cucumber stopped cancer cells from spreading and activated a process that killed the cells - and this happened within five minutes of the cells being exposed to the extract!The researchers concluded that sea cucumber extract causes cell cycle arrest and apoptosis (cell death) in human pancreatic cells. (doctorshealthpress.com)
  • Lead author and Brown graduate student Hetal Marble said targeting gene expression rather than surface proteins for the purpose of gathering cells to make a new tissue is a "paradigm shift" in the following regard: Gene expression provides a way to target any cell based on whether it can produce another tissue, while targeting surface proteins limits researchers to harvesting cells that fit a presumed definition of being a stem cell. (eurekalert.org)
  • GFP assay in cell-free extracts? (bio.net)
  • The CellTiter 96 ® AQ ueous One Solution Cell Proliferation Assay (CellTiter) was used to measure cell viability in response to antioxidant-rich extracts of Terminalia ferdinandiana fruits. (mdpi.com)
  • Conflicting results were obtained from this assay whereby higher concentrations of extracts significantly increased cell viability compared to lower concentrations. (mdpi.com)
  • Intrinsic reductive potential was observed in a cell-free system when extracts were added directly to the CellTiter assay reagent. (mdpi.com)
  • To confirm this effect in a similar cell proliferation assay, we employed the CellTiter-Blue ® Cell Viability Assay and again observed increased viability with increased concentrations of the extracts and direct reduction of the assay reagent by the extracts in cell-free systems. (mdpi.com)
  • In the search for a cell proliferation assay that would not be directly affected by the plant extracts, we identified the CyQUANT ® NF Cell Proliferation Assay that is based on the estimation of DNA content in viable cells. (mdpi.com)
  • Could you give me a protocol for Hela cell extract preparation which amenable for further GST pulldown assay?or from your viewpoints,whether it is a good way for screening binding partner?your kind help will be greatly appreciated. (protocol-online.org)
  • In our in vitro study, the effect of different concentrations (0, 50, 100, and 200 µg/mL) in HepG2 cells was evaluated using MTT assay. (magiran.com)
  • The safety of L. citriodora aqueous extract was also confirmed by cell viability assay. (magiran.com)
  • Cytotoxicity and increased intracellular calcium levels were determined using the Sulforhodamine B (SRB) assay and Fluo4-AM (acetoxymethyl) staining and fluorescence microscopy in NB cells in order to screen a library of plant extracts. (spandidos-publications.com)
  • In addition, the mitochondrial transition pore assay and western blot analysis revealed that cell death is associated with opening of the mitochondrial permeability transition pore (mPTP), and caspase-3 and PARP cleavage in NB cells. (spandidos-publications.com)
  • We used male and female Swiss mice and Wistar rats and the comet assay and micronucleus test to investigate the mutagenic potential of a crude extract of P. cubeba seeds. (scielo.br)
  • For the Wistar rats, peripheral blood and hepatic cells were collected for the comet assay and bone marrow cells were collected for the micronucleus test 24 h after treatment. (scielo.br)
  • These compounds were subjected to a sulphorhodamine B (SRB) cytotoxicity assay against human large cell lung carcinoma (COR-L23), and normal human fetal fibroblast (MRC-5), cell lines. (thefreelibrary.com)
  • This study investigated the in vitro anticancer effects of the ethanol extracts against human epithelial MCF-7 breast cancer cells in a dose-dependent manner (1-50 μg/ml) by using cell cycle analysis, viability assay, annexin V/PI staining, TUNEL method and expression determination of apoptotic and adhesion relevant proteins. (sigmaaldrich.com)
  • To investigate the hypothesis that nonsecretors express unique receptors for uropathogenic E. coli related to their genetic background, we extracted glycosphingolipids (GSLs) from vaginal epithelial cells collected from nonsecretors and secretors and used an assay in which radiolabeled uropathogenic E. coli were bound to these GSLs separated on TLC plates. (jci.org)
  • Cell viability was scored by use of MTT assay. (koreamed.org)
  • Cell viability of RAW264.7 cells treated with cranberry extract were analyzed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. (cranberryinstitute.org)
  • The cell viability was determined by the MTT assay. (arvojournals.org)
  • An MTT assay was used to evaluate the cell viability of five cancer cell lines and one normal cell line. (unboundmedicine.com)
  • Cell viability effects were completed by Annexin staining. (hindawi.com)
  • High AFA concentrations decreased viability of normal marrow cells. (hindawi.com)
  • The impact of plant extracts and phytochemicals on in vitro cell viability is usually assessed by employing cell viability assays dependent upon the activity of dehydrogenase enzymes. (mdpi.com)
  • Cell viability decreased with increasing concentrations of the extracts. (mdpi.com)
  • Accordingly, the results of the present study indicated that cell viability assays reliant upon dehydrogenase activity may lead to false positive results when testing antioxidant-rich plant extracts with intrinsic reductive potential, and alternative cell viability assays should be used to measure the cell viability. (mdpi.com)
  • In this study we show the biological effect of Echinacea augustifolia extract on cell viability and cell differentiation in mammary epithelial cell lines. (greenmedinfo.com)
  • Their findings are quite remarkable as they were able to demonstrate that thymoquinone was a potent inhibitor of oral cancer cell viability via two distinct anti-neoplastic mechanisms. (greenmedinfo.com)
  • Therefore, we used Fluo4-AM staining and fluorescence microscopy while concurrently performing Sulforhodamine B (SRB) staining on NB cells in order to determine the effect of this extract on intracellular calcium signaling and cell viability, respectively. (spandidos-publications.com)
  • The dichloromethane extract of Scrophularia orientalis significantly increased intracellular-free calcium levels and reduced NB cell viability. (spandidos-publications.com)
  • Moreover, carnosic acid caused the lowest cell viability with values ranging from 13 to 30 % at a concentration of 19 muM after 48 h of treatments, resulting in superior antiproliferative effect. (herbs.org)
  • Its biological activity on proliferation, viability and ROS protection was investigated towards K562, CaCo-2 and SH-SY5Y human cancer cell lines. (rsc.org)
  • Moreover, the viability of sodium iodate-treated APRE-19 cells was reduced. (arvojournals.org)
  • The viability of cells was assessed after 72 h incubation with various combinations of B and D using the trypan-blue staining method. (degruyter.com)
  • SFN exerts differential effects on cell proliferation, HDAC activity and downstream targets in normal and cancer cells. (nutraingredients-usa.com)
  • Using human breast cancer cells and primary human mammary epithelial cells in vitro, Ray and colleagues found the mechanism of bitter melon extract significantly decreased proliferation, that is, cell growth and division, and induced death in breast cancer cells. (massagemag.com)
  • The protein is also produced by cancer cells themselves, which can further their proliferation. (naturalnews.com)
  • Cell proliferation was inhibited in K562 and SH-SY5Y cells in the first 48 h at 500 μg mL −1 but slowly resumed after 72 h. (rsc.org)
  • Here, we report that the Tinospora cordifolia extracts (TCE) induced inhibition of proliferation of KB cells was associated with G0/G1-phase arrest. (omicsonline.org)
  • Several antigenic preparations derived from Schistosoma mansoni schistosomula were compared as immunogens in proliferation assays utilizing peripheral blood mononuclear cells (PBMN) from patients with chronic intestinal schistosomiasis mansoni. (ajtmh.org)
  • The bulb methanol extract of A. atroviolaceum was found to be an active cell proliferation inhibitor at the time and dose dependent manner. (frontiersin.org)
  • We've shown similar anti-cancer activity in the past with grape seed extract (GSE), but now we know B2G2 is its most biologically active ingredient which can be synthesized in quantities that will allow us to study the detailed death mechanism in cancer cells," says Alpna Tyagi, PhD, of the University of Colorado Skaggs School of Pharmacy and Pharmaceutical Sciences. (redorbit.com)
  • Eventually the group pinpointed B2G2 as the most active compound, but, "it's expensive and it takes a long time to isolate B2G2 from grape seed extract," Tyagi says. (redorbit.com)
  • An active ingredient of grape seed extract, B2G2, acts against cancer cells. (innovations-report.com)
  • While grape seed extract has shown activity in a number of laboratory cancer cell lines, including skin, breast, colon, lung, stomach and prostate cancers, no one had tested the extract in hematological cancers nor had the precise mechanism for activity been revealed. (goodnewsnetwork.org)
  • These results could have implications for the incorporation of agents such as grape seed extract into prevention or treatment of hematological malignancies and possibly other cancers," said the study's lead author, Xianglin Shi, Ph.D., professor in the Graduate Center for Toxicology at the University of Kentucky. (goodnewsnetwork.org)
  • What everyone seeks is an agent that has an effect on cancer cells but leaves normal cells alone, and this shows that grape seed extract fits into this category," he said. (goodnewsnetwork.org)
  • Based on those studies, and findings from other researchers that grape seed extract reduces breast tumors in rats and skin tumors in mice, they looked at the effect of the compound in leukemia cells. (goodnewsnetwork.org)
  • Alpna Tyagi, of University of Colorado Skaggs School of Pharmacy and Pharmaceutical Sciences, said that they have detected similar anti cancer action by grape seed extract in the past but only now they have found out that B2G2 is that active ingredient that when synthesized can trigger a death mechanism in cancerous cells. (themedguru.com)
  • The research group has been involved in this study over a decade to detect the anticancer action of grape seed extract (GSE) under controlled conditions in the laboratory. (themedguru.com)
  • Tyagi said that the research team had discovered this constituent present in the grape seed extract only recently. (themedguru.com)
  • The group of researchers also outlined that even though the component of grape seed extract B2G2 is highly active, it is a bit on the pricey side and takes a high time to isolate itself from the GSE. (themedguru.com)
  • Therefore, they decided to synthesize it in the lab instead of purifying it from grape seed extract. (themedguru.com)
  • Grape seed extract was shown to inhibit the growth and survival of colorectal cancer cells in advanced disease while leaving healthy cells undisturbed in a recent study. (oncologynurseadvisor.com)
  • We've known for quite a while that the bioactive compounds in grape seed extract selectively target many types of cancer cells," explained investigator Molly Derry in a statement from the University of Colorado in Denver. (oncologynurseadvisor.com)
  • Now, according to Derry, the findings demonstrate that many of the same mutations that allow colorectal cancer cells to metastasize and survive traditional therapies make them especially sensitive to treatment with grape seed extract. (oncologynurseadvisor.com)
  • Whereas generally much more chemotherapy is needed to kill a stage IV cancer cell than a stage II cell, Derry's group found the reverse to be true with grape seed extract. (oncologynurseadvisor.com)
  • It required less than half the concentration of [grape seed extract] to suppress cell growth and kill 50% of stage IV cells than it did to achieve similar results in the stage II cells," recounted Derry in the statement. (oncologynurseadvisor.com)
  • The researchers identified several contributors to colorectal cancer cell death induced by grape seed extract: oxidative stress, loss of mitochondrial membrane potential, modulation of pro- and anti-apoptotic proteins, and involvement of both caspase-dependent and caspase-independent apoptopic pathways. (oncologynurseadvisor.com)
  • Grape seed extract halts cell cycle, checking growth of colorectal tumo. (bio-medicine.org)
  • Their study, published in the October 18 issue of Clinical Cancer Research, documented a 44 percent reduction of advanced colorectal tumors in the animals, and also revealed, for the first time, the molecular mechanism by which grape seed extract works to inhibit cancer growth. (bio-medicine.org)
  • With these results, we are not suggesting that people run out and buy and use grape seed extract. (bio-medicine.org)
  • The value of this preclinical study is that it shows grape seed extract can attack cancer, and how it works, but much more investigation will be needed before these chemicals can be tested as a human cancer treatment and preventive," he said. (bio-medicine.org)
  • Agarwal and his team of investigators were first to report, in 1999, that grape seed extract also has chemopreventive activity against skin cancer. (bio-medicine.org)
  • Patients with colorectal cancer may benefit from the cancer-growth-inhibiting power of grape seed extract. (realfarmacy.com)
  • Grape seed extract is amazing in that it attacks the cancer cells but leaves the healthy cells untouched. (realfarmacy.com)
  • Actually, the power of grape seed extract seems quite remarkable in this research. (realfarmacy.com)
  • Molly Derry, a doctoral candidate in the lab of Rajesh Agarwal, PhD, and investigator at the CU Cancer Center and her team saw that while doses of chemotherapy only increase with more severe cancer cases, such as a stage IV instead of stage II, the amount of grape seed extract required actually decreased. (realfarmacy.com)
  • Grape seed extract is made from the seed of the grape and is beneficial for a number of cardiovascular conditions such as poor circulation and high cholesterol. (realfarmacy.com)
  • The bioactive compounds in grape seed extract selectively target many types of cancer cells. (realfarmacy.com)
  • But thankfully, as mentioned, researchers found that it required less grape seed extract to kill advanced cancer cells than it did cells in the early stage. (realfarmacy.com)
  • In the present study, we screened 138 medicinal plant extracts in TNBC cells to identify candidates that are not cytotoxic as a single agent but that can increase TRAIL-induced cytotoxicity synergistically. (spandidos-publications.com)
  • Evaluation of 138 plant extracts for their cytotoxicity as a single regimen and/or additive or synergistic effect with TRAIL. (spandidos-publications.com)
  • Gold nanoparticles do not induce myotube cytotoxicity but increase the susceptibility to cell death. (sigmaaldrich.com)
  • No cytotoxicity was observed in CaCo-2 cells under the same experimental conditions. (rsc.org)
  • The study confirms the very low cytotoxicity of the T. lanceolatus ethanolic extract and highlights its nutraceutical properties as an antioxidative and preservative agent and its possible use as an ingredient in functional foods. (rsc.org)
  • The results of the present study demonstrated that the polyphenol‑rich extract from S. chinensis induced potent cytotoxicity in the MCF‑7 human breast cancer cells, A549 human lung cancer cells, HCT‑116 and COLO 205 human colon cancer cells, and MiapaCa‑2 human PC cells. (unboundmedicine.com)
  • The cytotoxic data were analyzed by the combination index method of Chou and Talalay to establish synergy between B and D. The results indicated that an overall positive reduction in drug concentration was achieved by D when combined with B in its cytotoxicity profile in the three human cancer cells tested. (degruyter.com)
  • The synergistic cytotoxicity was best when the B:D ratio of 1:5 was used in PaCa cells at IC 50 , IC 75 and IC 90 dose levels and in MCF-7 cells at IC 90 dose level. (degruyter.com)
  • Results published in Advances in Bioscience and Biotechnology ​ ​ indicated that incubating human HeLa cells with the commercially available KSM-66 Ashwagandha root extract led to an enhancement of approximately 45% in telomerase activity at a concentration of 10 to 50 micrograms. (nutraingredients.com)
  • Using M13mp2 DNA containing the simian virus 40 (SV40) origin of replication and a plus-one frameshift mutation in the lacZ alpha reporter gene, we performed replication reactions using a HeLa cell extract and the SV40 large T antigen. (nih.gov)
  • Coommassie stained SDS-PAGE of 25 ?g of Doxorubicin stimulated Human Derived Hela Cell Nuclear Extract (Ready-to-Use) separated in a 4-20% gradient gel under non-reducing conditions. (antikoerper-online.de)
  • To demonstrate the efficiency of cellular fractionation using the Nuclear Extract Kit, nuclear, cytoplasmic, and whole-cell extracts were prepared from HeLa cell samples. (activemotif.com)
  • To demonstrate the quality of the materials and protocol of the Nuclear Extract Kit, nuclear and cytoplasmic extracts were prepared from HeLa cell samples that were either unstimulated or stimulated with TNF-α for 30 minutes. (activemotif.com)
  • NFκB activation was assayed with the TransAM ® NFκB p50 Kit using increasing amounts of nuclear and cytoplasmic extracts isolated from unstimulated and stimulated HeLa cells using the Nuclear Extract Kit. (activemotif.com)
  • This unit describes the preparation of nuclear extracts from cultured cells and optimized production of transcriptionally active extracts from HeLa cells. (currentprotocols.com)
  • The multiple therapeutic effects of natural compounds in traditional medicine motivate us to evaluate the cytotoxic activity of bulb of Allium atroviolaceum in MCF7 and MDA-MB-231, HeLa and HepG2 cell lines. (frontiersin.org)
  • Determination of DNA content by flow cytometry demonstrated S and G2/M phase arrest of MCF-7 cell, correlated to Cdk1 downregulation, S phase arrest in MDA-MB-231 which is p53 and Cdk1 -dependent, sub-G0 cell cycle arrest in HeLa aligned with Cdk1 downregulation, G0/G1, S, G2/M phase arrest in HepG2 which is p53-dependent. (frontiersin.org)
  • Further, the combination of the extract with tamoxifen against MCF7 and MDA-MB-231 and combination with doxorubicin against HeLa and HeG2 demonstrated synergistic effect in most concentrations, suggests that the bulb of A. atroviolaceum may be useful for the treatment of cancer lonely or in combination with other drugs. (frontiersin.org)
  • In HeLa cells, Pds5 proteins physically interact with cohesin and associate with chromatin in a cohesin-dependent manner. (biologists.org)
  • Although a wide variety of cytotoxic plant extracts and phytochemicals are known to act synergistically with anticancer drug doxorubicin (D), their clinical application is hindered by safety concerns of such combination therapy. (degruyter.com)
  • These data suggest that algae extracts may inhibit AML cell lines and leukemia blasts, but they may also have potential inhibitory effects on normal hematopoiesis. (hindawi.com)
  • Agarwal gave a note of caution, stating that while these results do provide hope as an anti-cancer agent, it is important to establish the validity of these results in animal models before adding them to one's diet to inhibit breast cancer cell growth. (massagemag.com)
  • And lastly, it has a preventive nature , as it can inhibit cancer appearance in those cells with a higher predisposition to develop it. (eurekalert.org)
  • Conclusion: Daphne Mucronata extract and its purified diterpene through increasing monocytes TNF-α releasing, potentially inhibit Leukemia cell line. (scirp.org)
  • We have investigated eosinophils as potential anti-cancer effector cells, and have reported the ability of their toxic granular proteins (MBP, EPO, ECP, EDN) to inhibit prostate tumor cell growth in vitro . (scirp.org)
  • Compared with the model group, cranberry extract could significantly inhibit the activity of NOS and decreased the content of IL-1 beta , IL-6, TNF- alpha with the increase of dose. (cranberryinstitute.org)
  • These results suggest that cranberry extract can inhibit the inflammatory response induced by lipopolysaccharide, and its mechanism may be related to activation of Keap1/Nrf2/HO-1 signaling pathway and NF- kappa Bp65. (cranberryinstitute.org)
  • This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. (sigmaaldrich.com)
  • The Nuclear Extract Kit isolates high-quality nuclear, cytoplasmic or whole-cell extract from mammalian cell or tissue samples. (activemotif.com)
  • Scientists from the Institute of Biochemistry and Biotechnology, Taiwan, have found that this remarkable compound elicits cytotoxic effects on various squamous cancer cell lines through various mechanisms. (greenmedinfo.com)
  • The results showed that after just 24 hours of treatment there was a significant concentration-dependent cytotoxic effect on these cells. (greenmedinfo.com)
  • LE exhibited a cytotoxic effect on Calu-3 cells and HumRBL-2H3 cells only at high concentrations and in a dose-dependent manner. (mdpi.com)
  • A recent study screened a library of 500 plant extracts for anti-proliferative and cytotoxic effects in neuroblastoma cell lines in order to identify new active substances for chemotherapy. (spandidos-publications.com)
  • Although the extracts exhibited various cytotoxic effects against different cell lines, comparatively low IC(50) values ranging between 12.50 and 47.55 microg/ml were attained against K-562, being the most sensitive cell line. (herbs.org)
  • Further, our recent comparative cytotoxic investigation with B and D in several human cancer cell lines indicated their potential for synergistic activity. (degruyter.com)
  • Different concentrations of B and D (0.29-290 μg/ml) and in various combinations (B:D ratio = 1:0, 1:1, 5:1, 1:5 and 0:1) were tested for cytotoxic effects against the three cancer cells. (degruyter.com)
  • The authors reported that SFN "selectively induced" ​ cell death in cancer cell lines, but not in healthy cell tissue. (nutraingredients-usa.com)
  • This makes adipose tissue, in theory, a readily available reservoir for regenerative therapies such as bone healing if doctors can get enough of those cells and compel them to produce bone. (eurekalert.org)
  • Meanwhile, the ALPL-expressing cells produced on average more than twice as much bone matrix (and as much as nine times more in some trials) during three weeks of subsequent cultivation than a similar-sized population of unsorted adipose tissue cells and almost four times more bone matrix than cells that don't express ALPL. (eurekalert.org)
  • A couple of other research groups have also sorted stem cells based on gene expression, but they have not done so specifically with the goal of enriching cell populations for a specific tissue, the researchers said. (eurekalert.org)
  • Clinicians continually discover new uses for stem cells, from the repair of damaged or failing organs, damaged tissue, and diseases, such as Type I diabetes. (bio-medicine.org)
  • Santa Cruz Biotechnology offers a variety of tissue extracts, whole cell lysates and nuclear extracts for use in combination with our antibodies as Western Blotting or Immunoprecipitation positive controls. (scbt.com)
  • For use as positive controls for phospho-specific antibodies, we offer Phospho-Enriched Whole Cell Lysates and Tissue Extracts. (scbt.com)
  • Stem cells extracted from body fat may pave the way for the development of new regenerative therapies including soft tissue reconstruction following tumor removal or breast mastectomy surgery, the development of tissue-engineered cartilage or bone, and the treatment of cardiovascular disease. (medicalxpress.com)
  • An interdisciplinary team of Queen's University researchers led by Dr. Lauren Flynn, a professor in the Departments of Chemical Engineering and Anatomy and Cell Biology, has been working with stem cells extracted from samples of human fat and is developing new methods in the lab to develop these cells into mature tissue substitutes. (medicalxpress.com)
  • Sarah Fleming, a Master's candidate in the group, is also working to establish a new method for growing the fat stem cells in the lab using a system that mimics the natural tissue environment found within the body. (medicalxpress.com)
  • This work is based on Dr. Flynn's development of a technique for washing away all traces of cells from a sample of body fat, leaving behind a three-dimensional tissue scaffold that she calls "decellularized adipose tissue", or "DAT" for short. (medicalxpress.com)
  • This empty scaffold can then be used for soft tissue reconstruction or as a growing environment for the extracted stem cells. (medicalxpress.com)
  • Dr. Flynn's preliminary studies have shown that when the stem cells are grown on the DAT scaffold, they naturally begin to mature into fat cells , suggesting that the engineered growth environment influences the type of cell that the basic stem cells will turn into during the tissue regeneration process. (medicalxpress.com)
  • In this study, the inner and outer regions of the dermis were separated and used to make two different tissue extracts. (biologists.org)
  • Microscopic image of the subcuticular tissue of the pleopod from a shrimp infected with the supernatant of the third passage of a human larynx carcinoma (Hep-2C) cell culture inoculated with an extract of shrimp infected with Taura syndrome virus. (cdc.gov)
  • The Nuclear Extract Kit works with fresh or frozen cell and tissue samples and yields protein amounts of approximately 0.5 to 1 mg from cytoplasmic extract (at 1-2 mg/ml concentration), 150 to 250 μg from nuclear extract (at 3-5 mg/ml concentration), 1.2 to 2.4 mg from whole cell extract (at 4-8 mg/ml concentration) from 8.8 x 10 6 cells. (activemotif.com)
  • Regional lymph node cells of mongrel dogs which received kidney homografts after bilateral nephrecto-my take up fluorescein-isothiocyanate-labeled extracts of kidney tissue and of peripheral blood leukocytes. (sciencemag.org)
  • These fluorochrome-tissue conjugates were not taken up by cells from lymph nodes excisedfrom the experimental dogs at the time of transplantation or from normal dogs. (sciencemag.org)
  • Adult stem cells are the unique source for skin tissue renewal. (unboundmedicine.com)
  • This step is necessary to equalize the amount of cells (and protein) in a given volume of whole cell extract. (unc.edu)
  • If the cells are alive on the chip, which they are, and if you have a new 'smart' drug that's supposed to attack a particular protein, you can test in the cell if the protein is being attached by the drug," he said. (health24.com)
  • Although current textbook explanations of cell-cycle control in eukaryotes emphasize the periodic activation of cyclin-dependent protein kinases (CDKs), recent experimental observations suggest a significant role for the periodic activation and inactivation of a CDK-counteracting protein phosphatase 2A with a B55δ subunit (PP2A:B55δ), during mitotic cycles in frog-egg extracts and early embryos. (nih.gov)
  • They found that the extract activates JNK, a protein that regulates the apoptotic pathway. (goodnewsnetwork.org)
  • The protein levels of Grp78 and Grp94 increased significantly in mice after bilberry extract administration. (dovepress.com)
  • Bilberry extract administration increased Grp78 and Grp94 protein levels, an effect which may underlie the neuroprotective effect of bilberry extract after optic nerve crush. (dovepress.com)
  • In this study we compared two different codon usages of RHDV2-VP1 to improve the expression of recombinant VP1 of RHDV2 by recombinant baculoviruses after infection of insect SF9 cells or transduction of mammalian RK13 cells in order to gain high protein yields. (springer.com)
  • Protein extracts were fractionated on Sephadex G-50 columns, and prostate tumor cell lines DU-145, LNCaP, PC-3, and HPC8L (established in our laboratory from a tumor resected from an African American patient) were treated with MBP extracts from the pooled third peaks. (scirp.org)
  • Furthermore, the development of cell-free expression systems has demonstrated the ability to reconstitute the protein production, transcription and translation processes (DNA→RNA→protein) in a controlled manner, harnessing synthetic biology. (jove.com)
  • Most often, the purpose of heterologous cell-free synthesis is to produce properly folded and functionally active protein product in the amounts sufficient for structural and functional studies. (frontiersin.org)
  • The developed method is intended for analysis of output from an existing cell-free protein production pipeline. (frontiersin.org)
  • A protein-polysaccharide complex (1 ® 6) b -D-glucan has been isolated from crude aqueous extracts of the fruiting body of the Basidiomycete A. blazei . (scielo.br)
  • Extracts prepared using the Nuclear Extract Kit can be used with Active Motif's TransAM ® Kits , or in other procedures that require cell extracts, like gelshift assays, Western blots, DNA footprinting, as a starting point for protein purification for binding or activity assays or or other analysis techniques. (activemotif.com)
  • The global protein extracts from single cell protein and other conventional sources market size is expected to reach USD 26.3 billion by 2025, according to a new report by Grand View Research, Inc. Registering an 8.4% CAGR during the forecast period, the market is expected to be driven by rising investments in the animal feed industry to cater to rising demand for cattle feed. (reportlinker.com)
  • India ranks first with respect to cattle inventory, with demand for cattle inventory constantly increasing in U.S. This is driving demand for animal feed and protein extracts from different sources. (reportlinker.com)
  • Moreover, there are a number of methods for the removal of ribonucleic acid (RNA) from extracted protein, another factor likely to impact market growth. (reportlinker.com)
  • Animal feed protein extracts and agricultural protein extracts are anticipated to grow at a similar pace in the coming years owing to rising demand for proteins in these sectors. (reportlinker.com)
  • North America accounts for the largest share in the protein extracts market with respect to pharmaceutical applications. (reportlinker.com)
  • Asia Pacific accounted for the largest share in protein extract consumption with regard to animal feed. (reportlinker.com)
  • The microarray is also of high sensitivity, as it allows the detection of a rare DNA-binding protein from breast cancer cells, the human tumor suppressor AP-2. (epfl.ch)
  • The Western blot result showed that cranberry extract inhibited the expression of Keap1, IKK alpha / beta , NF- kappa Bp65 and increase the expression of Nrf2 and HO-1 protein levels. (cranberryinstitute.org)
  • The authors found that it increases availability of a critical protein, Cip1/p21, in tumors that effectively freezes the cell cycle, and often pushes a cancer cell to self destruct. (bio-medicine.org)
  • Membrane vesicles from schistosomula were found to elicit mean proliferative responses similar to those stimulated by a CaCl 2 -extracted tegument preparation and to do so at lower total protein concentrations. (ajtmh.org)
  • Although these results are not considered to provide direct evidence, they are interpreted to mean that (-)-kaurene metabolism in these extracts is protected from inhibition by insoluble PVP. (oregonstate.edu)
  • Results: Fifty percent of growth inhibition was shown by 160 μL (1:100 dilution, 0.5 g of the powdered leaves/ml) of the extract and 0.94 nM of the purified component, and there was more inhibition in K562 cells (P (scirp.org)
  • Four fold increases in growth inhibition was shown in co culture of isolated human monocytes and leukemia cell lines. (scirp.org)
  • M. Mianabadi and R. Yazdanparast, "Inhibition of Substrate-Tumor Cell Adhesion under the Effect of Gnidilatimonoein Purified from Daphne Mucronata," American Journal of Chinese Medicine, Vol. 32, No. 3, 2004, pp. 369-376. (scirp.org)
  • One ml of the cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g (wet weight) of bovine liver, or 10 mL of cell lysate obtained from CHO cells at a cell density of 10 8 cells per mL. (sigmaaldrich.com)
  • The purpose of this study is to elucidate combination effect of cisplatin and green tea extracts on the growth inhibition of TC-1 cell. (koreamed.org)
  • RESULTS: Cell growth inhibition in a dose dependent was observed at the different concentration of ciaplatin, GTP and EGCG. (koreamed.org)
  • Also, in the groups treated by 0.5 uM of cisplatin and GTP (1 and 5 ug/ml) or EGCG (25 and 50 uM), the inhibition of cell growth showed with 12.2%, 6.9% and 63.4%, 72.2% as compared to the group treated by cisplatin only. (koreamed.org)
  • CONCLUSION: Additive effect of the combination of cisplatin with GTP or EGCG on the inhibition of cell growth was observed. (koreamed.org)
  • The Colo 205 and MCF‑7 cancer cell lines were the most susceptible to treatment with the extract, which exhibited increased rate of growth inhibition. (unboundmedicine.com)
  • They studied the growth inhibition and death in human pancreatic cells. (doctorshealthpress.com)
  • Effects against these oral cancer types were seen in-vitro (cell culture) and in-vivo (xenograft mouse model). (greenmedinfo.com)
  • R. P. Singh, K. N. C. Murthy and G. K. Jayaprakasha, "Studies on Antioxidant Activity of Pomegranate (Punica granatum) Peel and Seed Extracts Using in Vitro Models," Journal of Agricultural and Food Chemistry, Vol. 50, No. 1, 2002, pp. 81-86. (scirp.org)
  • The extracts from these mushrooms were isolated in chloroform:methanol (3:1) and used in vitro at three different concentrations. (scielo.br)
  • These extracts are able to recapitulate accurate transcription initiation and splicing in vitro, which has been useful in direct functional studies. (currentprotocols.com)
  • There is a selective toxicity in vitro to cancer-cell lines derived from the lungs, liver, and colon, compared to normal cell lines. (scielo.cl)
  • It offers a powerful cell-free (or in vitro) system for studying various cell biological processes, including cell cycle progression, nuclear transport, DNA replication and chromosome segregation. (wikipedia.org)
  • Extracts from Ashwagandha root may significantly increase telomerase activity, thereby protecting against telomere loss and potentially delaying aging, suggest new results from a cell study. (nutraingredients.com)
  • When tested against Erlotinib in a non-small-cell lung cancer model, the Noni leaf extract significantly increased healthy gene response and decreased unhealthy gene response, increased anti-inflammatory expression in the lungs and enhanced antioxidant responses against oxidative injuries. (davidwolfe.com)
  • Gene expression of Chop , Bax , and Atf4 increased in mice after optic nerve crush and decreased significantly after oral bilberry extract administration. (dovepress.com)
  • Tested A. Melanocarpa extract significantly inhibited the expression of ICAM-1 and VCAM-1, attenuated the phosphorylation of NF-κB p65 and decreased intracellular ROS production in TNFα-treated HAECs. (biomedsearch.com)
  • This extract contained highly potent agents that significantly reduced cell survival and increased calcium levels in NB cells. (spandidos-publications.com)
  • PHILADELPHIA − Chemicals found in grape seeds significantly inhibited growth of colorectal tumors in both cell cultures and in mice, according to researchers who have already demonstrated the extract's anti-cancer effects in other tumor types. (bio-medicine.org)
  • For example, previous studies have shown the GSE effectiveness against cancer cells and have also shown its mechanism of action. (redorbit.com)
  • This naturally occurring compound, GSE, is a complex mixture of polyphenols and also so far it has been unclear about the biologically active constituents of GSE against cancer cells," Tyagi says. (redorbit.com)
  • During cell replication, the telomeres function by ensuring the cell's chromosomes do not fuse with each other or rearrange, which can lead to cancer. (nutraingredients.com)
  • The safety, and cancer-targeting ability of the broccoli extract sulforaphane, has been backed by a new study finding that it can selectively target cancer cells. (nutraingredients-usa.com)
  • The research, published in Molecular Nutrition and Food Research ​, reports for the first time that sulforaphane - one of the primary phytochemicals in broccoli and other cruciferous vegetables - is able to selectively target and kill cancer cells, while leaving normal cells healthy and unaffected. (nutraingredients-usa.com)
  • "It is well documented that sulforaphane can target cancer cells through multiple chemopreventive mechanisms," ​said the authors, led by Dr Emily Ho, associate professor at the Linus Pauling Institute at Oregon State University, USA. (nutraingredients-usa.com)
  • "But ​[sulforaphane] does appear to be a phytochemical that can selectively kill cancer cells," ​ she affirmed. (nutraingredients-usa.com)
  • They added that their results "provide further support for the relevance of sulforaphane as a dietary HDAC inhibitor and chemopreventive ​[cancer preventing] agent" ​ by showing that sulforaphane can selectively target cancer cells while leaving normal cells virtually unaffected. (nutraingredients-usa.com)
  • An experimental process that snags lung cancer cells from a blood sample could give doctors real-time feedback on the most effective therapy, researchers reported. (health24.com)
  • Dr Daniel Haber of the Massachusetts General Hospital Cancer Centre and Harvard Medical School and colleagues were able to extract blood-borne cancer cells from 27 volunteers with non-small-cell lung cancer that had spread. (health24.com)
  • They found that changes in the number of circulating cancer cells correlated with the effectiveness of a patient's treatment and were also able to track genetic changes in the tumour cells over time. (health24.com)
  • The study, reported on the Web site of the New England Journal of Medicine , is another step in the quest for individualised medicine, where doctors strive to quickly assess a tumour, choose the most effective treatment, and alter that treatment as cancer cells adapt. (health24.com)
  • The CTC chip, licensed to the privately held CellPoint Diagnostics in Mountain View, California, is 100 times more sensitive than a US Food and Drug Administration-approved technique that uses magnetic beads to try to extract cancer cells, according to Haber. (health24.com)
  • It takes about eight hours to send the blood across the 80 000 tiny columns so specially designed antibody glue can latch onto passing cancer cells. (health24.com)
  • Extracts of turmeric - a condiment commonly used in Indian cuisine - can be rendered soluble and delivered to tumours to kill cancer cells, scientists say. (deccanherald.com)
  • Recently, scientists have also discovered that curcumin, a naturally occurring substance isolated from turmeric, is an effective agent for killing cancer cells. (deccanherald.com)
  • The study, published in the journal PNAS, showed that the complex was 100 times more effective in treating various cancer types such as melanoma and breast cancer cells than using curcumin and platinum agents separately. (deccanherald.com)
  • Curcumin has shown to prevent the phosphorylation of STAT3, a well-known signalling pathway that triggers the growth of cancer cells and allows them to survive, in vivo studies. (deccanherald.com)
  • Pan's team also hopes to prove that this method will be effective in killing cancer stem cells, in effect cancer's root system, preventing the diseases from recurring in patients. (deccanherald.com)
  • Preclinical study results - published ​ in the journal ​ Integrative Cancer Therapies ​ - showed two compounds extracted from seaweed were found to halt the cell cycle, and prevent cancer cells from dividing and spreading, said Marinova spokesperson Claire Smoorenburg. (in-pharmatechnologist.com)
  • "In an oncology setting, fucoidan [seaweed extract] acts via a number of cell pathways leading to cell cycle arrest in the first growth phase (G1) or cancer cell lines," ​she told us. (in-pharmatechnologist.com)
  • The firm now has a human interaction study underway in the US, which aims to further investigate fucoidan extracts in combination with chemotherapy to assess the safety and quality of life parameters in cancer patients, said Smoorenburg. (in-pharmatechnologist.com)
  • PHILADELPHIA - Bitter melon extract, a common dietary supplement, exerts a significant effect against breast cancer cell growth and may eventually become a chemopreventive agent against this form of cancer, according to results of a recent study. (massagemag.com)
  • Our findings suggest that bitter melon extract modulates several signal transduction pathways, which induces breast cancer cell death," said lead researcher Ratna B. Ray, Ph.D., professor in the Department of Pathology at Saint Louis University. (massagemag.com)
  • This extract can be utilized as a dietary supplement for the prevention of breast cancer. (massagemag.com)
  • This study may provide us with one more agent as an extract that could be used against breast cancer if additional studies hold true. (massagemag.com)
  • Additional studies are needed to further understand the molecular targets of bitter melon extract in cancer cells, as well as for establishing its in vivo efficacy. (massagemag.com)
  • Ray and colleagues are currently conducting follow-up studies using a number of cancer cell lines to examine the anti-proliferative effect of the extract. (massagemag.com)
  • Natural News ) Could a simple fruit extract really help kill cancer? (naturalnews.com)
  • Galectin-3 is one of the most studied galectins, and research continues to show that it is both directly and indirectly connected to cancer cell activity and may contribute to cancer progression in a number of different ways, including cancer progression and metastasis. (naturalnews.com)
  • Essentially, it makes it easier for cancer cells to adhere to the walls of blood vessels, and in this way, helps the cancer spread. (naturalnews.com)
  • Studies have also shown that MCP can stimulate the immune system and boost production of natural killer cells, which can locate and destroy cancer cells. (naturalnews.com)
  • For example, a study published in 2015 by the journal PLOS One found that MCP induced cell death in two different types of cancer cells. (naturalnews.com)
  • Another study, published by BMC Complementary and Alternative Medicine in 2011 found that MCP activated natural killer cells that were then able to target and destroy leukemic cancer cells. (naturalnews.com)
  • The researchers analyzed the lipophilic extracts of whole tomatoes to determine their impact on the neoplastic features of cell lines in gastric cancer. (naturalnews.com)
  • They discovered that extracts taken from two tomato varieties in particular, San Marzano and Corbarino, could stop malignant stomach cancer cells from cloning and growing. (naturalnews.com)
  • Small cell lung cancer: Is milk thistle extract the key to defeat? (cityofhope.org)
  • Small cell lung cancer is an aggressive type of cancer that readily metastasizes to other organs in the body. (cityofhope.org)
  • The disease can be tough to treat, because the cancer cells often develop resistance to the drugs commonly used against them. (cityofhope.org)
  • Study shows silibinin, an extract of the milk thistle plant, reverses drug resistance in small cell lung cancer. (cityofhope.org)
  • Susan Kane, Ph.D., a professor in City of Hope's Department of Cancer Biology , and David Sadava, Ph.D., a visiting professor from the Claremont Colleges, compared effects of the extract silibinin on two specific cancer cell lines that have shown resistance to three common chemotherapeutics - etoposide, doxorubicin and vincristine. (cityofhope.org)
  • Various milk thistle extracts have been used as natural supplements to help protect the liver and treat diabetic neuropathy, and recent studies of silibinin have shown it to have some anti-cancer activity against prostate tumors. (cityofhope.org)
  • Additionally, after incubation with silibinin for 96 hours, the cancer cells showed that their drug resistance to the three chemotherapeutics had reversed. (cityofhope.org)
  • If further research confirms the findings, small cell lung cancer patients who find their tumors no longer responding to chemotherapy could get a second chance against the disease, leading to longer lives for patients. (cityofhope.org)
  • Silibinin, a non-toxic natural product, may be useful in treatment of drug-resistant [small cell lung cancer]. (cityofhope.org)
  • THIS Plant Extract Kills Lung Cancer Cells Better than Chemo! (davidwolfe.com)
  • The study found that extract from a Noni leaf was more effective than the chemotherapy drug known as Erlotinib, when it came to suppressing metastasized lung cancer in an animal model. (davidwolfe.com)
  • Lung cancer takes 1.2 million lives each year, and about 80% of these lung cancer cases are classified as non-small-cell lung cancer (NSCLC) , which conventional chemotherapy and/or radiotherapy is rarely able to kill, due to chemoresistance and radioresistance. (davidwolfe.com)
  • This new study evaluated the effects of Noni leaves on metastasized lung cancer development in both a cell model and an animal model, and compared the results with Erlotinib, using two different types of lung cancer cells. (davidwolfe.com)
  • The extract also suppressed inflammation and increased antioxidant response, and the response of apoptotic genes that suppress cancer. (davidwolfe.com)
  • DENVER--(BUSINESS WIRE)--Jan 19, 2012 - Cannabis Science, Inc. (OTCBB: CBIS) a pioneering U.S. biotech company developing pharmaceutical cannabis (marijuana) products, is very pleased to announce that the second patient suffering from basal cell carcinoma (skin cancer) has self-administered cannabis extracts that appear to have eradicated the cancer. (drugs.com)
  • Following up on the December 12, 2011 news release regarding a second cancer patient self-administering cannabis extract, this patient has now given the photo-documented dramatic results of treatment process. (drugs.com)
  • While the treatment has been completed and the cancer seems to be gone, Cannabis Science is waiting for a full biopsy report from the physician to confirm the patient is free of cancer cells. (drugs.com)
  • Currently it appears that the aforementioned patient will be able to avoid any surgical procedures such as, standard surgical excision (Mohs Surgery), and other various types of treatment for skin cancer, like chemotherapy, radiation, and cryosurgery, by using the cannabis extracts, just like the first patient mentioned in our March 9, 2011 news release. (drugs.com)
  • This finding prompted us to search for other natural products that can sensitize cancer cells to TRAIL or that can overcome the TRAIL-resistant mechanism. (spandidos-publications.com)
  • This component when synthesized in huge quantities can cause it to deal with the cancer cells in prostate glands. (themedguru.com)
  • Last time, they discovered the effectiveness of GSE in dealing with cancer cells and outlined its action mechanism. (themedguru.com)
  • Nanowerk News ) An international group led by scientists at UCLA s California NanoSystems Institute has developed a new method for effectively extracting and analyzing cancer cells circulating in patients blood ( 'Programming Thermoresponsiveness of NanoVelcro Substrates Enables Effective Purification of Circulating Tumor Cells in Lung Cancer Patients' ). (nanowerk.com)
  • Circulating tumor cells are cancer cells that break away from tumors and travel in the blood, looking for places in the body to start growing new tumors called metastases. (nanowerk.com)
  • Capturing these rare cells would allow doctors to detect and analyze the cancer so they could tailor treatment for individual patients. (nanowerk.com)
  • The device, developed at UCLA, enables scientists to control the blood s temperature the way coffeehouses would with an espresso machine to capture and release the cancer cells in optimal conditions. (nanowerk.com)
  • Because it could make extracting the cancer cells much more efficient and cost-effective at a time in a patient s life when information is needed as quickly as possible, Tseng said it is conceivable that the new system will replace laser capture microdissection as the standard protocol. (nanowerk.com)
  • With our new system, we can control the blood s temperature the way coffeehouses would with an espresso machine to capture and then release the cancer cells in great purity, said Tseng, who is also a member of UCLA s Jonsson Comprehensive Cancer Center. (nanowerk.com)
  • This shows the translational value of our device in managing non small-cell lung cancer with underlying mutations. (nanowerk.com)
  • M. Hedayati, R. Yazdanparast, M. Yeganeh, L. Rad and F. Azizi, "A New Diterpene Extracted from Daphne Mucronata, Effects on Human K562 and CCRF-CEM Cell Lines," Journal of Cancer Therapy , Vol. 2 No. 1, 2011, pp. 71-75. (scirp.org)
  • Lymphotoxin β receptor activation promotes mRNA expression of RelA and pro-inflammatory cytokines TNFα and IL-1β in bladder cancer cells. (sigmaaldrich.com)
  • This Plant Extract Forces Cancer Cells to Commit Suicide in 24 Hours! (davidwolfe.com)
  • Pamela Ovadje, a post-doctoral fellow at the University of Windsor, has done extensive work in investigating the anti-cancer properties of dandelions and other natural extracts. (davidwolfe.com)
  • Well, we should all be very glad that researchers started looking into dandelion root extract and its effects on cancer because the results seem almost too good to be true. (davidwolfe.com)
  • Since the commencement of this project, we have been able to successfully assess the effect of a simple water extract of dandelion root in various human cancer cell types in the lab, and we have observed its effectiveness against human T-cell leukemia, chronic myelomonocytic leukemia, and pancreatic and colon cancers, with no toxicity to non-cancer cells. (davidwolfe.com)
  • Furthermore, these efficacy studies have been confirmed in animal models (mice) that have been transplanted with human colon cancer cells. (davidwolfe.com)
  • Dr. Siyaram Pandey, professor of chemistry and biochemistry at the University of Windsor and principal research investigator for the project, believes dandelion root extract has "good potential" to kill cancer cells in the human body. (davidwolfe.com)
  • Basically, the extract causes cancer cells to kill themselves while leaving healthy cells alone. (davidwolfe.com)
  • This is far better than current chemotherapy drugs that kill one healthy cell for every 5 to 10 cancer cells . (davidwolfe.com)
  • It should be understood that the concentration of the extract is much higher than what is currently available on the market, but it is important to remember that this all started when one oncologist noticed dandelion root tea helped cancer patients. (davidwolfe.com)
  • Trials are still underway, but this fantastic extract may be the future of cancer treatment! (davidwolfe.com)
  • He explains how dandelion root extract causes the cancer cells to commit suicide just 24 hours after it is introduced into the body. (davidwolfe.com)
  • As she and her colleagues reported in Cancer Letters , they experimented on colorectal cancer cell lines representing various stages of the disease. (oncologynurseadvisor.com)
  • Moreover, the efficacy of this treatment increased as the metastatic potential of the cancer cells increased. (oncologynurseadvisor.com)
  • For this reason, "Finding a way to selectively target advanced colorectal cancer cells could have major clinical importance," she affirmed. (oncologynurseadvisor.com)
  • root extracts against a lung cancer cell line (COR-L23). (thefreelibrary.com)
  • Pro-apoptotic and anti-adhesive effects of four African plant extracts on the breast cancer cell line MCF-7. (sigmaaldrich.com)
  • The three extracts, termed PS, JCP1 and JCP2 at a concentration of 10 μg/ml induced cell death in MCF-7 breast cancer cells verified by high amounts of PI-positive cells in the cell cycle analysis, Annexin V/PI staining and DNA fragmentation measurements. (sigmaaldrich.com)
  • However, all four plant extracts mediated strong anti-cancer properties with IC50 values between 23-38 μg/ml. (sigmaaldrich.com)
  • PS, JCP1 and JCP2 were found to be very active against MCF-7 cells by inducing anoikis and therefore possessing vast potential as medicinal drugs especially in estrogen receptor positive breast cancer treatment. (sigmaaldrich.com)
  • Inhibitory effects of rosemary extracts, carnosic acid and rosmarinic acid on the growth of various human cancer cell lines. (herbs.org)
  • Rosemary extract is a potential candidate to be included in the anti-cancer diet with pre-determined doses avoiding toxicity. (herbs.org)
  • Moreover, raspberry extracts contain a high level of ellagitannins, which upon hydrolysis release ellagic acid, conferring antiviral properties and protection against certain forms of cancer. (cosmeticsandtoiletries.com)
  • A n international group led by scientists at UCLA's California NanoSystems Institute has developed a new method for effectively extracting and analyzing cancer cells circulating in patients' blood. (healthcanal.com)
  • One essential strategy for cancer therapy is to activate apoptotic pathways in the tumor cells. (frontiersin.org)
  • Fluorescence microscopy, using 4',6‑diamidino‑2‑phenylindole as a staining agent, was performed in order to detect the morphological changes of the MiapaCa‑2 cancer cells and the presence of apoptotic bodies following treatment with the extract. (unboundmedicine.com)
  • Researchers from the University of Colorado Cancer Center report that the more aggressive the cancer cells are, the more effective the grape extract works at targeting and stopping the growth. (realfarmacy.com)
  • This is a departure from conventional cancer treatments that destroy all of the cells in an attempt to stop the spread of cancer. (realfarmacy.com)
  • This study shows that many of the same mutations that allow colorectal cancer cells to metastasize and survive traditional therapies make them especially sensitive to treatment with GSE. (realfarmacy.com)
  • It is thought that the extract kills cancer cells by a process of oxidative stress. (realfarmacy.com)
  • They recommended that, because sea cucumber extract is edible and nontoxic, it may be valuable for nutritional therapy or prevention of pancreatic cancer . (doctorshealthpress.com)
  • Because of these effects, the extract is commonly used in folk medicines as a remedy for diabetes in such locales as India, China and Central America, according to the researchers. (massagemag.com)
  • Stem cell research has expanded exponentially in recent years, as researchers across the globe continue to discover exciting new applications. (bio-medicine.org)
  • An extract from grape seeds forces laboratory leukemia cells to commit cell suicide, according to researchers from the University of Kentucky. (goodnewsnetwork.org)
  • Now City of Hope researchers may have found a way to overcome this drug resistance - by using an extract of the milk thistle plant. (cityofhope.org)
  • Researchers pointed out, "Chemotherapy and/or irradiation usually fail because NSCLC cells are intrinsically resistant to them. (davidwolfe.com)
  • The researchers concluded that "the 300 mg/kg extract was more effective than the 50 mg/kg Erlotinib for most of the parameters measured. (davidwolfe.com)
  • Researchers from Advanced Cell Technology have generated stem cell cultures by plucking individual cells from newly fertilized embryos, which are not harmed. (elixirnews.com)
  • The whole tomato extracts stopped the cells from migrating away from the primary tumor, causing them to die . (naturalnews.com)
  • The E. purpurea-mediated increase in NK cell numbers was indeed paralleled by an increase in their anti-tumor, lytic functional capacity. (greenmedinfo.com)
  • In his laboratory at the UCLA California NanoSystems Institute, Hsian-Rong Tseng, a professor of molecular and medical pharmacology, used a device he invented to capture circulating tumor cells from blood samples. (nanowerk.com)
  • The device, called the NanoVelcro Chip, is a postage-stamp sized chip with nanowires that are 1,000 times thinner than a human hair and are coated with antibodies that recognize circulating tumor cells. (nanowerk.com)
  • When 2 milliliters of blood are run through the chip, the tumor cells stick to the nanowires like Velcro. (nanowerk.com)
  • Capturing the tumor cells was just part of the battle, though. (nanowerk.com)
  • Now Tseng and his colleagues have developed a thermoresponsive NanoVelcro purification system, which enables them to raise and lower the temperature of the blood sample to capture (at 37 degrees Celsius) and release (at 4 degrees Celsius) circulating tumor cells at their optimal purity. (nanowerk.com)
  • In the PA1-treated group, the TC-1 cells had necrosis, whereas in the control groups the tumor cells were undamaged. (scielo.cl)
  • Our earlier studies showed that red beetroot ( Beta vulgaris L.) extract (B), approved by Food and Drug Administration and European Union as red food color E162, reduced multi-organ tumor formations in various animal models when administered in drinking water. (degruyter.com)
  • Since B is considered safe for human use with no known toxicity, we conducted the present study to evaluate its synergistic antiproliferative activity with D against pancreatic (PaCa), breast (MCF-7) and prostate (PC-3) tumor cells of human origin. (degruyter.com)
  • In turn, these cancerous cells can continue to multiply, and produce more galectin-3. (naturalnews.com)
  • Research suggests that this suppresses tumors by isolating damaged parts of a cell and even promoting cell death of cancerous cells. (greenmedinfo.com)
  • R. Yazdanparast and H. Sadeghi, "Nucleic Acid Synthesis in Cancerous Cells under the Effect of Gnidilatimonoein from Daphne Mucronata," Life Sciences, Vol. 74, No. 15, 2004, pp. 1869-1876. (scirp.org)
  • Further, resistance of cancerous cells to a specific drug which initially suppressed them is a major problem of chemotherapy drugs. (frontiersin.org)
  • Therefore, this study attempted to identify novel plant extracts that have anticancer effects. (spandidos-publications.com)
  • The current study examined the anticancer effects of a dichloromethane extract from Scrophularia orientalis L. (Scrophulariaceae), a plant that has been used in Traditional Chinese Medicine. (spandidos-publications.com)
  • The current study examines one extract from Scrophularia orientalis L., as it was shown to have potent anticancer effects in NB cells. (spandidos-publications.com)
  • In the current study, a Scrophularia orientalis dichloromethane extract was found to have anticancer effects in NB, and was further examined to elucidate these effects. (spandidos-publications.com)
  • The present study indicates that this extract be used as an alternative strategy for treating NB, and may lead to the development of more effective anticancer agents for NB. (spandidos-publications.com)
  • Hence GA was purified further through RP-HPLC and evaluated its anticancer potential by studying its effect on mitochondrial respiration, cell-membrane integrity, apoptotic body formation and the DNA fragmentation in cultured A549 cells. (scirp.org)
  • The toxicity of anticancer drugs to normal fast-growing cells limits their efficacy. (frontiersin.org)
  • The aim of the present study was to evaluate the anticancer potential of a polyphenol enriched extract obtained from Salvia chinensis, a Chinese medicinal plant. (unboundmedicine.com)
  • Role for regulated phosphatase activity in generating mitotic oscillations in Xenopus cell-free extracts. (nih.gov)
  • In Xenopus egg extracts, mitotic chromosomes assembled in the absence of Pds5A and Pds5B display no discernible defects in arm cohesion, but centromeric cohesion is apparently loosened. (biologists.org)
  • Xenopus egg extract is a lysate that is prepared by crushing the eggs of the African clawed frog Xenopus laevis. (wikipedia.org)
  • It is also called Xenopus egg cell-free system or Xenopus egg cell-free extract. (wikipedia.org)
  • Later, the same procedure was applied to eggs of Xenopus laevis, becoming popular for studying cell cycle progression and cell cycle-dependent cellular events. (wikipedia.org)
  • Extracts derived from eggs of the Japanese common toad Bufo japonicus or of the Western clawed frog Xenopus tropicalis have also been reported. (wikipedia.org)
  • As shown in Figure 1, proteins that serve as markers for cellular fractionation, such as nuclear marker RNA pol II and cytoplasmic marker alpha Tubulin, were only detected in their respective cellular fractions and in whole-cell extracts. (activemotif.com)
  • Active Motif also offers a number of ready-to-use nuclear, cytoplasmic and whole-cell extracts . (activemotif.com)
  • The 2018 PDA Cell and Gene Therapy Conference (October 23-24) will provide a broad overview of current innovations with a focus on what it takes to successfully steward these therapies toward approval and beyond. (pharmaceuticalonline.com)
  • This approach was designed to determine the DNA-binding activity of proteins in crude cell extracts, complementing conventional expression profiling arrays. (epfl.ch)
  • Our nuclear extracts are suitable as either positive controls for Western Blotting or for use in Gel Shift Assays. (scbt.com)
  • Comparative assays of (-)-kaurene biosynthesis and accumulation in cell-free extracts of excised seed coats, cotyledons and embryonic shoot-root axes revealed that the enzymes responsible for (-)-kaurene biosynthesis apparently are localized exclusively in the cotyledons. (oregonstate.edu)
  • The identity of SGG in the nonsecretor GSL extracts was confirmed in radioimmunoassays using an mAb to SGG and in immunofluorescence assays with this mAb and native vaginal epithelial cells. (jci.org)
  • Extracts are suitable for use in a various applications including EMSA, Western blot and binding assays and is ideally suited for preparation of extracts for our TransAM ® Transcription Factor Assays . (activemotif.com)
  • Genotoxic effects of an aqueous extract of Nicotiana tabacum, a variety commonly used in India for chewing purposes, were analysed on CHO cells utilizing two different cytogenetic end-points, namely, chromosome aberration frequency and sister chromatid exchange frequency. (nih.gov)
  • The present study evaluated the acute, subacute, and cellular toxicity of the aqueous extract of L. citriodora leaves. (magiran.com)
  • The acute and subacute toxicity of the plant was evaluated with a single intraperitoneal (IP) injection of aqueous extract at the dosage range of 0, 1, 2, and 5 g/kg of body weight (acute model) in mice and rats and 21 days administration at the dosage range of 0, 50, 100, and 200 mg/kg of body weight. (magiran.com)
  • Results from the present study elucidated that treatment with the aqueous extract of L. citriodora leaves was well tolerated via daily IP injection at doses up to 200 mg/kg for a period of 21 days and did not produce any toxicity. (magiran.com)
  • Aqueous extract of human placenta, used as wound healer, has shown significant cell adhesion property on mouse peritoneal macrophages and P 388D1 cultured macrophage cell line. (niscair.res.in)
  • The composition of methanolic leek extract (LE) was analyzed by GC-MS and LC-IT/MS, and the total phenolic content and antioxidant capacity were quantified by colorimetric methods. (mdpi.com)
  • As the endothelial cell expression of adhesion molecules has been recognised as an early step in inflammation and atherogenesis, we examined the effect of walnut methanolic extract and ellagic acid, one of its major polyphenolic components (as shown by HPLC analysis), on the expression of vascular cell adhesion molecule (VCAM)-1 and intracellular adhesion molecule (ICAM)-1 in human aortic endothelial cells. (cambridge.org)
  • The leaves of Rosmarinus officinalis harvested from three different locations of Turkey were extracted by both methanolic and supercritical CO(2) extraction. (herbs.org)
  • In this study, GC-MS analysis was carried out for the methanolic extract of pomegranate peel which revealed gallic acid (GA) as the major antioxidant compound in the extract. (scirp.org)
  • While Nissan's approach offers the flexibility of using fuels ranging from natural gas to ethanol to ethanol-water blends, an onboard reformation step is necessary to extract the hydrogen needed for the SOFC from any hydrocarbon fuel. (caranddriver.com)
  • Ethanol extracts were prepared of Dunaliella salina (Dun), Astaxanthin (Ast), Spirulina platensis (Spir), and Aphanizomenon flos-aquae (AFA). (hindawi.com)
  • The pigment-extracted JSC4 residues increased ethanol production by 10.7% and 31.6% compared to raw starch and whole JSC4 cells, respectively. (rsc.org)
  • Overall, this study successfully demonstrates a feasible cell-surface display fermentation system for use in direct high-density ethanol production from pigment-extracted microalgal material. (rsc.org)
  • The cell lines were grown in a lab and treated with different concentrations of thymoquinone. (greenmedinfo.com)
  • METHODS AND RESULTS: Human aortic endothelial cells (HAECs) were pretreated with various concentrations (primarily 50 μg/mL) of Aronia Melanocarpa fruit extract prior to treatment with TNFα (10 ng/mL) for various periods of time. (biomedsearch.com)
  • We observed increased level of reactive oxygen species in the cells treated with GA at the concentrations of 10 and 20 ug/ml. (scirp.org)
  • Even at the lowest concentrations (50 μg mL −1 ), the extract was efficiently able to protect human cells against t -BHP-induced oxidative damage. (rsc.org)
  • In December, the same group reported in Nature that their circulating tumour cells, or CTC, chip could extract malignant cells from people with breast, prostate, pancreatic and colorectal cancers, as well as lung tumours. (health24.com)
  • The enzymes catalyzing the synthesis of (-)-kaurene from meva- Ionic acid were present in the soluble fraction of these extracts, whereas one preliminary experiment indicated that the enzymes catalyzing (-)-kaurene oxidation were localized in a microsomal fraction. (oregonstate.edu)
  • In the present paper, an approach to identify the multiple physicochemical and structural properties of amino acid sequences associated with soluble expression of eukaryotic proteins in cell-free bacterial extracts is presented. (frontiersin.org)
  • Using this method, a number of significant correlations between calculated and predicted properties of amino acid sequences and their propensity for soluble cell-free expression have been revealed. (frontiersin.org)
  • In the present paper, we describe an approach aimed at identification of numerous physicochemical, structural and functional properties of amino acid sequences, including the sites of multiple PTMs, associated with soluble expression of eukaryotic proteins in bacterial cell-free extracts, and highlight major correlations obtained using this approach. (frontiersin.org)
  • Here, the focus is set on the processing of experimental data with the purpose of identification of multiple physicochemical and structural properties associated with soluble expression of eukaryotic proteins in cell-free bacterial extracts. (frontiersin.org)
  • Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. (currentprotocols.com)
  • DNA‐dependent transcription of adenovirus genes in a soluble whole‐cell extract. (currentprotocols.com)
  • A soluble fraction that appears between the lipid cap and the yolk is called an M phase extract. (wikipedia.org)
  • Bacterial extracts are widely used to synthesize recombinant proteins. (frontiersin.org)
  • However, the folding of eukaryotic proteins is greatly compromised in bacterial extracts due to intrinsic differences between the cytoplasmic environments of prokaryotic and eukaryotic cells. (frontiersin.org)
  • Seaweed extracts can reduce tumour growth and improve the effectiveness of chemotherapy says Marinova, which is developing the compounds for pharmaceutical applications. (in-pharmatechnologist.com)
  • The extract caused changes consistent with those of an excellent chemotherapy agent. (davidwolfe.com)
  • The Noni leaf extract also exhibited no toxicity in normal lung cells - something that chemotherapy and radiation cannot match, as they can damage healthy cells in the process. (davidwolfe.com)
  • Green tea extract (GTE) has been shown to be anti-oxidative in different disease models. (arvojournals.org)
  • In reverse, GTE rescued the cell death and oxidative stress in ARPE-19 cells as well as the disorganization of outer nuclear layer in rats. (arvojournals.org)
  • Aronia melanocarpa fruit extract exhibits anti-inflammatory activity in human aortic endothelial cells. (biomedsearch.com)
  • The liquid stem cells come from a frozen stem cell extract that is consumed orally. (bio-medicine.org)
  • Exuviance is among the first to create a skin care product with apple stem cell extract to help stimulate and preserve healthy cells for younger looking skin. (beautyinthebag.com)
  • A stem cell extract was prepared as described below, starting from the leaves of wild red raspberry since they showed the highest antioxidant content of all the plant organs. (cosmeticsandtoiletries.com)
  • A new dermocosmetic product containing apple stem cell extract, urea, creatine and palmitoyl tripeptide-38 (Ureadin Fusion Serum Lift Antiarrugas, ISDIN S.A), has been recently developed to target different aspects involved in skin aging. (unboundmedicine.com)
  • Biosynthesis of beta-glucans by cell-free extracts from Saccharomyces cerevisiae. (biomedsearch.com)
  • Cell-free extracts from Saccharomyces cerevisiae catalyzed the incorporation of glucosyl residues from UDP-[U-14C]glucose into beta-1,3-glucans which contained a significant proportion of beta-1,6-glycosidic linkages. (biomedsearch.com)
  • Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae. (currentprotocols.com)
  • In this study, we successfully developed a consolidated bioprocessing (CBP) system using recombinant Saccharomyces cerevisiae displaying synergistic cellulases/amylases on cell surfaces to overcome energy-conversion limitations. (rsc.org)
  • Collectively, the data indicate that E. purpurea, at least, and possibly other plant compounds, appear to contain phytochemicals capable of stimulating de novo production of NK cells, as well as augmenting their cytolytic function, in animals of advanced age. (greenmedinfo.com)
  • Washed cells were then incubated on ice in lysis buffer containing 10 mM HEPES, 60 mM KCl, 1.0 mM EDTA, 0.075% (v/v) NP40 and 1.0 mM DTT, pH 7.6. (antikoerper-online.de)
  • There are many different lysis buffers for mammalian cells dependent on which proteins you are interested in. (protocol-online.org)
  • This work demonstrates the ability of Protea cell lysis kits, based on acid labile surfactant chemistries, to effectively extract proteins from peanut samples with very high yield. (selectscience.net)
  • The aging and lifespan of normal, healthy cells are linked to the so-called telomerase shortening mechanism, which limits cells to a fixed number of divisions. (nutraingredients.com)
  • Our cells have a safety mechanism of programmed death which kick in if they become disordered, as usually happens when they become malignant. (greenmedinfo.com)
  • Thymoquinone was found to induce this mechanism of programmed death in the squamous cell carcinoma cells, effectively making them shut down and die. (greenmedinfo.com)
  • It is the term given to a cellular mechanism in which intracellular components are isolated within the cell and then destroyed. (greenmedinfo.com)
  • To develop combination therapies to improve TRAIL efficacy and/or to overcome the resistant mechanism, we screened 138 medicinal plant extracts against TRAIL-sensitive and -insensitive TNBC cell lines, MDA-MB-231 and MDA-MB-468. (spandidos-publications.com)
  • Additionally, to determine details of the mechanism of the neuroprotective effect of bilberry extract anthocyanins and the involvement of endoplasmic reticulum stress suppression in the mouse retina. (dovepress.com)
  • Hence the present study was planned to explore the molecular mechanism of the anticarcinogenic activity pomegranate peel on A549 cell line. (scirp.org)
  • To study the anti-inflammatory effect of cranberry extract on inflammation suppression induced by lipopolysaccharide, and explore its mechanism. (cranberryinstitute.org)
  • To evaluate the efficiency of cellular fractionation, 20 μg of nuclear (Nu), cytoplasmic (Cy) and whole-cell (WC) extracts, along with nuclear pellet collected during nuclear extraction using the Nuclear Extract Kit were analyzed by Western blot using antibodies against various specific proteins. (activemotif.com)
  • Figure 2: Specific extraction of nuclear and cytoplasmic extracts. (activemotif.com)
  • Cell-cycle analysis of AML cell lines showed G0/G1 arrest in the presence of AFA. (hindawi.com)
  • There was no influence of different codon usages on RHDV2-VP1 gene expression in the respective cell lines detected. (springer.com)
  • So in this study, the effect of Daphne Mucronata purified diterpene were investigated on co culture of human monocytes and two human leukemia cell lines (K562, CCRF-CEM). (scirp.org)
  • Briefly, granular proteins were differentially extracted from GRC.014.22 and GRC.014.24, eosinophilic cell lines established in our laboratory from a patient with moderate asthma. (scirp.org)
  • Here we demonstrate that TSV also infects human cell lines, which may suggest that Penaeus is a potential reservoir of this virus. (cdc.gov)
  • Since Sabin strain LSc 2ab (Sabin 1), the poliovirus used for human vaccination, is usually replicated in monolayer culture cells of human rhabdomyosarcoma (RD), human larynx carcinoma (Hep-2C) (), or Buffalo green monkey kidney (BGM) ( 7 ), we injected these cell lines with a 0.22-µm membrane-filtered whole extract of the hepatopancreas of shrimp ( Penaeus stylirostris ) affected with TSV. (cdc.gov)
  • Control cell lines were injected with filtered hepatopancreas extracts from healthy shrimp. (cdc.gov)
  • Fresh cell lines were inoculated with the lysate, incubated, and processed in a similar way. (cdc.gov)
  • Materials and Methods: Each cell line mono-layer culture, in log phase growth, was treated with 10 to 160 μL of the extract (1 g/ml leave powder) and purified compound (0.94 nM). (scirp.org)
  • 9 Finally, the extracts include the important compound resveratrol, a polyphenol whose activity has been linked to anticarcinogenic effects and the regulation of low-density lipoproteins, mediated by sirtuin activation. (cosmeticsandtoiletries.com)
  • Simulations of normal mitotic cycles in a frog-egg extract. (nih.gov)
  • 12, 13 Prolonged and chronic UV exposure can cause alterations and damage to the various skin cell types-i.e., epidermal keratinocytes, melanocytes, dermal fibroblasts and even cells of the immune system, such as macrophages and neutrophils, which can transiently reside in the skin. (cosmeticsandtoiletries.com)
  • Epidermal and dermal stem cells compartments are fundamental for the continuous renewal of the skin. (unboundmedicine.com)
  • The major products of colicin E1 plasmid DNA synthesis in cell extracts are completely replicated molecules and a class of molecules containing newly synthesized small DNA fragments. (pnas.org)
  • The addition of 10% glycerol and/or 2 mM spermidine to extracts blocks synthesis of completely replicated molecules while enhancing synthesis of molecules containing newly synthesized DNA fragments. (pnas.org)
  • This new study is based on the synthesis of B2G2, an active component present in the extracts of grape seed. (themedguru.com)
  • Procedures were developed for assaying the biosynthesis of (-)- kaurene-¹⁴C and other intermediates in gibberellin (GA) synthesis from mevalonic acid-¹⁴C in cell-free enzyme extracts of immature pea (Pisum sativum L. cv. (oregonstate.edu)
  • The rates of (-)-kaurene synthesis and accumulation were greater in the 100,000 x g supernatant fraction than in the 40,000 x g supernatant fraction of extracts of excised cotyledons, whereas the opposite relationship was observed with extracts of whole seeds. (oregonstate.edu)
  • We examined the effects of algae extracts on normal hematopoietic cells and leukemia cells. (hindawi.com)
  • We focused on defining the effects of Echinacea purpurea extracts in dendritic cells (DCs), which generate innate and adaptive immune responses. (greenmedinfo.com)
  • These effects have been observed in two different cell line derived from mouse (HC11) and bovine (BME-UV). (greenmedinfo.com)
  • Statistically significant elevations in the values of both the markers clearly indicated chromosome damaging effects of the extract. (nih.gov)
  • Interestingly, the group also examined the effect on normal oral mucosal cells and found no significant inhibitory effects from thymoquinone. (greenmedinfo.com)
  • Here, the authors evaluate a D. biflorus cell culture extract for its effects on skin cells. (cosmeticsandtoiletries.com)
  • To investigate the effects of the extract on skin, it is important to first consider mechanisms of the skin aging process. (cosmeticsandtoiletries.com)
  • Noni leaf extract can cost as little as 12 cents per 250 mg, has no negative side effects, and has been proven to aid in the process of healing. (davidwolfe.com)
  • Among the tested extracts, 5 extracts (Uvaria dac, Artemisia vulgaris, Cortia depressa, Dichasia bengalensis and Cinnamomum obtusifolium) were found to activate TRAIL effects in TRAIL-sensitive TNBC MDA-MB-231 cells and to overcome unresponsiveness in TRAIL-insensitive TNBC MDA-MB-468 cells. (spandidos-publications.com)
  • CONCLUSION: We conclude that A. Melanocarpa fruit extract exhibits anti-inflammatory effects in HAECs by inhibiting the expression of endothelial CAMs, activation of NF-κB and production of ROS. (biomedsearch.com)
  • M. Hedayati, R. Yazdanparast and F. Azizi, "Daphne Mucronata Extracts Effects on down Regulation of TNF-α Receptors on Cultured Human Monocytes," Yakhte, Vol. 27, No. 7, 2005, pp. 152-157. (scirp.org)
  • Under the circumstances of this study, these organic extracts did not show any genotoxic or mutagenic effects, but did protect cells against the induction of micronuclei by MMS. (scielo.br)
  • Nevertheless, since some Basidiomycetes species are poisonous, it is necessary to obtain epidemiological and experimental data on the beneficial effects of the extracts of the species studied. (scielo.br)
  • To address this issue, we examined the effects of an extract (bag cell extract, BCE) that contained BCPs, as well as egg-laying hormone (ELH), an identified BCP, on both the tail-siphon withdrawal reflex and the ability of that reflex to exhibit sensitization, a simple form of nonassociative learning. (jneurosci.org)
  • These effects were not observed in animals receiving an extract of a control ganglion or in animals receiving ELH, and therefore may be attributed to the actions of one or more BCPs, but presumably not to ELH alone. (jneurosci.org)
  • RD, Hep-2C, and BGM cells injected with an extract similarly processed but from healthy shrimp, showed no cytopathic effects, even after 7 days of culture ( Figures 1A , 1C , and 1 E ). As a positive control, RD cells were injected with Sabin viral extract and showed the characteristic cytopathic effect produced by an enterovirus infection. (cdc.gov)
  • Plants have stem cells and plant derived stem cell extracts are now used in topical products for their potential anti-ageing and anti-wrinkle effects. (unboundmedicine.com)
  • In our study we were interested in evaluating, for the first time, the morphological effects of administration of PA1 to implanted TC-1 cells and in the target organs in vivo. (scielo.cl)
  • T-REx293 cells expressed the nicotinic acetylcholine receptor (nAchR) and tubocurarine, an nAChR antagonist, completely blocked the effects of nicotine. (go.jp)
  • In addition, the effects of the extract on apoptotic induction, cell cycle phase distribution, DNA damage and loss of mitochondrial membrane potential (ΛΨm) were evaluated in MiapaCa‑2 human PC cells. (unboundmedicine.com)
  • The effects of the extract on cell cycle phase distribution and ΛΨm were assessed by flow cytometry, using propidium iodide and rhodamine‑123 DNA‑binding fluorescent dyes, respectively. (unboundmedicine.com)
  • Solar cells based on perovskite compounds could soon make electricity generation from sunlight even more efficient and cheaper. (innovations-report.com)
  • In relation, recent studies have shown that plant cell culture extracts in general are rich sources for compounds including phenols, flavonoids and their derivatives, which impart a wide range of efficacy and benefits for cosmetic formulations such as neutralizing free radical damage. (cosmeticsandtoiletries.com)
  • Further, the work presented reinforces cell culture systems as efficient biofactories to produce natural and effective compounds. (cosmeticsandtoiletries.com)
  • Yeast cells are heated until they rupture, then the cells' own digestive enzymes break their proteins down into simpler compounds (amino acids and peptides), a process called autolysis. (wikipedia.org)
  • The umbilical cord of a newborn contains a large amount of the baby's own stem cells, which can be effectively stored for future use. (bio-medicine.org)
  • Patients self-administering cannabis extracts in the convenience of their own home appear to be effectively resolving their cancerous lesions over a period of a few weeks to a few months. (drugs.com)
  • When saline extracts from schistosomular bodies submitted to these two treatments were used as stimulants in parallel PBMN cultures it was found that vesiculation more effectively removed antigens involved in the stimulation of PBMN. (ajtmh.org)
  • The scientists of the UGR have characterized for the first time maslinic acid action from the molecular point of view when it is applied to the development of tumour cells. (eurekalert.org)
  • Hello, I look forward methods to extract hydrophobic proteins from yeast wall cells. (protocol-online.org)
  • This is the first published study to use a standardized, branded ashwagandha extract that show an anti-aging effect with a telomerase promotion effect in the human cell line. (nutraingredients.com)
  • These indications prompted us, therefore, to investigate the potentially immuno-stimulating effect of one such phytocompound, Echinacea purpurea, on natural killer (NK) cells since these cells are active in spontaneous, non-specific immunity against neoplasms and virus-mediated infections. (greenmedinfo.com)
  • To investigate the effect of bilberry extract anthocyanins on retinal ganglion cell (RGC) survival after optic nerve crush. (dovepress.com)
  • The present results suggest that the walnut extract has a high anti-atherogenic potential and a remarkable osteoblastic activity, an effect mediated, at least in part, by its major component ellagic acid. (cambridge.org)
  • Background: Daphne Mucronata extract has a decreasing effect on the size of breast adenocarcinoma in rats. (scirp.org)
  • Supercritical CO(2) extracts had superior antiproliferative effect compared to the soxhlet extracts. (herbs.org)
  • The Mn-liver extract had no effect on incorporation of thymidine-5′-triphosphate into DNA but blocked the phosphorylation of deoxythymidine. (aacrjournals.org)
  • The cytopathic effect in RD cells began with a partial destruction of the cellular layer. (cdc.gov)
  • The cytopathic effect in the BGM cell line began as a partial destruction of the cellular layer, which evolved to a syncytial-like formation of rounded, refringent cells. (cdc.gov)
  • The effect of cranberry extract on nucleus was observed by 4',6-diamidino-2-phenylindole(DAPI)staining. (cranberryinstitute.org)
  • There was no significant toxic effect on RAW246.7 cells in the range of 5 micro g/mL-400 micro g/mL, and the cell nucleus was intact and without obvious damage. (cranberryinstitute.org)
  • We demonstrated for the first time that there is a selective toxic effect of PA1 on the TC-1 cells in vivo. (scielo.cl)
  • Flow cytometric analysis demonstrated that the extract induced G0/G1 cell cycle arrest in a dose‑dependent manner. (unboundmedicine.com)
  • In a new study in the journal Stem Cell Research & Therapy , scientists at Brown University demonstrate a new method for extracting a wide variety of potential bone-producing cells from human fat. (eurekalert.org)
  • The present study was designed to assess the numbers/production of NK cells in the spleen and bone marrow of aging, normal mice, after in vivo dietary administration of E. purpurea (14 days), or, after injection of thyroxin, a stimulant of NK cell function (10 days). (greenmedinfo.com)
  • In the current study, we tested the hypothesis that Ginkgo biloba extract (GBE) prevented hyperinsulinism-induced glucose intolerance in hepatocytes. (herbs.org)
  • [ii] thymoquinone induced significant amounts of autophagic activity in the squamous cell carcinoma cells examined in the study. (greenmedinfo.com)
  • In the present study, we sought a role of walnuts ( Juglans regia L.) in endothelial and bone-cell function. (cambridge.org)
  • This randomized, placebo-controlled, double-blind study evaluates the efficacy and tolerability of the allogeneic human adipose derived stem cell component extract on androgenic alopecia in relatively healthy adults. (clinicaltrials.gov)
  • This study aimed to investigate the ethanolic extract of T. lanceolatus , a species native to north-western Algeria, traditionally used as herbal tea, seasoning and a preservative for meat and poultry. (rsc.org)
  • Our model is consistent with what is already known about the regulation of CDK and PP2A:B55δ in frog eggs, and it suggests a previously undescribed role for the Greatwall-PP2A:B55δ interaction in creating a toggle switch for activation of the anaphase-promoting complex as embryonic cells exit mitosis and return to interphase. (nih.gov)
  • and (3) further metabolism of (-)-kaurene in extracts of immature pea seeds. (oregonstate.edu)
  • Repeated efforts to demonstrate cell-free metabolism of exogenous (-)-kaurene resulted in uniformly negative results. (oregonstate.edu)
  • The product seemed to optimize the metabolic functions in human senescent cultured fibroblast restoring a more efficient cell metabolism therefore contributing to the anti-aging properties of the product. (unboundmedicine.com)
  • In this paper, we extend an earlier mathematical model of embryonic cell cycles to include experimentally motivated roles for PP2A:B55δ and its regulation by Greatwall kinase. (nih.gov)
  • Hardline critics of embryo research, however, are unlikely to accept the manipulation even of a single embryonic cell, which they say could theoretically become a human being. (elixirnews.com)
  • Upon fertilization, the metaphase arrest is released by the action of Ca2+ ions released from the endoplasmic reticulum, thereby initiating early embryonic cell cycles that alternates S phase (DNA replication) and M phase (mitosis). (wikipedia.org)
  • Binding of uropathogenic Escherichia coli R45 to glycolipids extracted from vaginal epithelial cells is dependent on histo-blood group secretor status. (jci.org)
  • We show that SGG and DSGG are selectively expressed by epithelial cells of nonsecretors, presumably as a result of sialylation of the gal-globoside precursor glycolipid, which in secretors is fucosylated and processed to ABH antigens. (jci.org)
  • The peptides released from the bag cells (bag cell peptides, BCPs) in Aplysia californica are responsible for eliciting or modulating a repertoire of behaviors preceding and accompanying egg laying. (jneurosci.org)