Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.
Relating to the size of solids.
Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.
A biocompatible polymer used as a surgical suture material.
The preparation, mixing, and assembling of a drug. (From Remington, The Science and Practice of Pharmacy, 19th ed, p1814)
Forms to which substances are incorporated to improve the delivery and the effectiveness of drugs. Drug carriers are used in drug-delivery systems such as the controlled-release technology to prolong in vivo drug actions, decrease drug metabolism, and reduce drug toxicity. Carriers are also used in designs to increase the effectiveness of drug delivery to the target sites of pharmacological actions. Liposomes, albumin microspheres, soluble synthetic polymers, DNA complexes, protein-drug conjugates, and carrier erythrocytes among others have been employed as biodegradable drug carriers.
A polyester used for absorbable sutures & surgical mesh, especially in ophthalmic surgery. 2-Hydroxy-propanoic acid polymer with polymerized hydroxyacetic acid, which forms 3,6-dimethyl-1,4-dioxane-dione polymer with 1,4-dioxane-2,5-dione copolymer of molecular weight about 80,000 daltons.
Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.
Deacetylated CHITIN, a linear polysaccharide of deacetylated beta-1,4-D-glucosamine. It is used in HYDROGEL and to treat WOUNDS.
Dosage forms of a drug that act over a period of time by controlled-release processes or technology.
A normal intermediate in the fermentation (oxidation, metabolism) of sugar. The concentrated form is used internally to prevent gastrointestinal fermentation. (From Stedman, 26th ed)
Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.
Chemistry dealing with the composition and preparation of agents having PHARMACOLOGIC ACTIONS or diagnostic use.
Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.
Structures which are part of the CELL MEMBRANE or have cell membrane as a major part of their structure.
The application of scientific knowledge or technology to pharmacy and the pharmaceutical industry. It includes methods, techniques, and instrumentation in the manufacture, preparation, compounding, dispensing, packaging, and storing of drugs and other preparations used in diagnostic and determinative procedures, and in the treatment of patients.
Hard or soft soluble containers used for the oral administration of medicine.
A series of progressive, overlapping events, triggered by exposure of the PLATELETS to subendothelial tissue. These events include shape change, adhesiveness, aggregation, and release reactions. When carried through to completion, these events lead to the formation of a stable hemostatic plug.
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.
Constituent composed of protein and phospholipid that is widely distributed in many tissues. It serves as a cofactor with factor VIIa to activate factor X in the extrinsic pathway of blood coagulation.
The process of the interaction of BLOOD COAGULATION FACTORS that results in an insoluble FIBRIN clot.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
Substances made up of an aggregation of small particles, as that obtained by grinding or trituration of a solid drug. In pharmacy it is a form in which substances are administered. (From Dorland, 28th ed)
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Platelet membrane glycoprotein IIb is an integrin alpha subunit that heterodimerizes with INTEGRIN BETA3 to form PLATELET GLYCOPROTEIN GPIIB-IIIA COMPLEX. It is synthesized as a single polypeptide chain which is then postranslationally cleaved and processed into two disulfide-linked subunits of approximately 18 and 110 kDa in size.
Polymerized forms of styrene used as a biocompatible material, especially in dentistry. They are thermoplastic and are used as insulators, for injection molding and casting, as sheets, plates, rods, rigid forms and beads.
A trace element that constitutes about 27.6% of the earth's crust in the form of SILICON DIOXIDE. It does not occur free in nature. Silicon has the atomic symbol Si, atomic number 14, and atomic weight [28.084; 28.086].
Term used to designate tetrahydroxy aldehydic acids obtained by oxidation of hexose sugars, i.e. glucuronic acid, galacturonic acid, etc. Historically, the name hexuronic acid was originally given to ascorbic acid.
Agents that cause clotting.
Anhydride polymers with a repeating structure of RC(=O)OC(=O)R. They readily hydrolyze in water making them useful for DELAYED-ACTION PREPARATIONS.
A sugar acid formed by the oxidation of the C-6 carbon of GLUCOSE. In addition to being a key intermediate metabolite of the uronic acid pathway, glucuronic acid also plays a role in the detoxification of certain drugs and toxins by conjugating with them to form GLUCURONIDES.
Characteristics or attributes of the outer boundaries of objects, including molecules.
Completed forms of the pharmaceutical preparation in which prescribed doses of medication are included. They are designed to resist action by gastric fluids, prevent vomiting and nausea, reduce or alleviate the undesirable taste and smells associated with oral administration, achieve a high concentration of drug at target site, or produce a delayed or long-acting drug effect.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours.
Poly-2-methylpropenoic acids. Used in the manufacture of methacrylate resins and plastics in the form of pellets and granules, as absorbent for biological materials and as filters; also as biological membranes and as hydrogens. Synonyms: methylacrylate polymer; poly(methylacrylate); acrylic acid methyl ester polymer.
Usually inert substances added to a prescription in order to provide suitable consistency to the dosage form. These include binders, matrix, base or diluent in pills, tablets, creams, salves, etc.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
Method of using a polycrystalline powder and Rietveld refinement (LEAST SQUARES ANALYSIS) of X-RAY DIFFRACTION or NEUTRON DIFFRACTION. It circumvents the difficulties of producing single large crystals.
Condition of having pores or open spaces. This often refers to bones, bone implants, or bone cements, but can refer to the porous state of any solid substance.
Differential thermal analysis in which the sample compartment of the apparatus is a differential calorimeter, allowing an exact measure of the heat of transition independent of the specific heat, thermal conductivity, and other variables of the sample.
Salts of alginic acid that are extracted from marine kelp and used to make dental impressions and as absorbent material for surgical dressings.
Platelet membrane glycoprotein complex essential for normal platelet adhesion and clot formation at sites of vascular injury. It is composed of three polypeptides, GPIb alpha, GPIb beta, and GPIX. Glycoprotein Ib functions as a receptor for von Willebrand factor and for thrombin. Congenital deficiency of the GPIb-IX complex results in Bernard-Soulier syndrome. The platelet glycoprotein GPV associates with GPIb-IX and is also absent in Bernard-Soulier syndrome.
Water swollen, rigid, 3-dimensional network of cross-linked, hydrophilic macromolecules, 20-95% water. They are used in paints, printing inks, foodstuffs, pharmaceuticals, and cosmetics. (Grant & Hackh's Chemical Dictionary, 5th ed)
Formation and development of a thrombus or blood clot in the blood vessel.
A complex mixture of PHOSPHOLIPIDS; GLYCOLIPIDS; and TRIGLYCERIDES; with substantial amounts of PHOSPHATIDYLCHOLINES; PHOSPHATIDYLETHANOLAMINES; and PHOSPHATIDYLINOSITOLS, which are sometimes loosely termed as 1,2-diacyl-3-phosphocholines. Lecithin is a component of the CELL MEMBRANE and commercially extracted from SOYBEANS and EGG YOLK. The emulsifying and surfactant properties are useful in FOOD ADDITIVES and for forming organogels (GELS).
Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.
Colloids formed by the combination of two immiscible liquids such as oil and water. Lipid-in-water emulsions are usually liquid, like milk or lotion. Water-in-lipid emulsions tend to be creams. The formation of emulsions may be aided by amphiphatic molecules that surround one component of the system to form MICELLES.
Colloids with a gaseous dispersing phase and either liquid (fog) or solid (smoke) dispersed phase; used in fumigation or in inhalation therapy; may contain propellant agents.
Antibodies that are chemically bound to a substrate material which renders their location fixed.
A network of cross-linked hydrophilic macromolecules used in biomedical applications.
Removal of moisture from a substance (chemical, food, tissue, etc.).
The administration of drugs by the respiratory route. It includes insufflation into the respiratory tract.
An enzyme formed from PROTHROMBIN that converts FIBRINOGEN to FIBRIN.
A cell line established in 1962 from disaggregated Swiss albino mouse embryos. This fibroblast cell line is extremely popular in research.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.
Technique whereby the weight of a sample can be followed over a period of time while its temperature is being changed (usually increased at a constant rate).
Cell adhesion molecule and CD antigen that mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells.
Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.
Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.
A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.
Coating with a metal or alloy by electrolysis.
The design or construction of objects greatly reduced in scale.
The testing of materials and devices, especially those used for PROSTHESES AND IMPLANTS; SUTURES; TISSUE ADHESIVES; etc., for hardness, strength, durability, safety, efficacy, and biocompatibility.
Cell adhesion molecules present on virtually all monocytes, platelets, and granulocytes. CD31 is highly expressed on endothelial cells and concentrated at the junctions between them.
Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.
A cellulose derivative which is a beta-(1,4)-D-glucopyranose polymer. It is used as a bulk laxative and as an emulsifier and thickener in cosmetics and pharmaceuticals and as a stabilizer for reagents.
Suspensions of killed or attenuated microorganisms (bacteria, viruses, fungi, protozoa), antigenic proteins, synthetic constructs, or other bio-molecular derivatives, administered for the prevention, amelioration, or treatment of infectious and other diseases.
A chlorinated hydrocarbon that has been used as an inhalation anesthetic and acts as a narcotic in high concentrations. Its primary use is as a solvent in manufacturing and food technology.
The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.
A product formed from skin, white connective tissue, or bone COLLAGEN. It is used as a protein food adjuvant, plasma substitute, hemostatic, suspending agent in pharmaceutical preparations, and in the manufacturing of capsules and suppositories.
A nonimmunologic, chemically induced type of photosensitivity producing a sometimes vesiculating dermatitis. It results in hyperpigmentation and desquamation of the light-exposed areas of the skin.
High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.
The process by which blood or its components are kept viable outside of the organism from which they are derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
Derivatives of caproic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a carboxy terminated six carbon aliphatic structure.
Methylester of cellulose. Methylcellulose is used as an emulsifying and suspending agent in cosmetics, pharmaceutics and the chemical industry. It is used therapeutically as a bulk laxative.
Two-phase systems in which one is uniformly dispersed in another as particles small enough so they cannot be filtered or will not settle out. The dispersing or continuous phase or medium envelops the particles of the discontinuous phase. All three states of matter can form colloids among each other.
A type of extracellular vesicle, containing RNA and proteins, that is secreted into the extracellular space by EXOCYTOSIS when MULTIVESICULAR BODIES fuse with the PLASMA MEMBRANE.
Homogeneous liquid preparations that contain one or more chemical substances dissolved, i.e., molecularly dispersed, in a suitable solvent or mixture of mutually miscible solvents. For reasons of their ingredients, method of preparation, or use, they do not fall into another group of products.
The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
A property of the surface of an object that makes it stick to another surface.
Endogenous substances, usually proteins, that are involved in the blood coagulation process.
A CHROMATOGRAPHY method using supercritical fluid, usually carbon dioxide under very high pressure (around 73 atmospheres or 1070 psi at room temperature) as the mobile phase. Other solvents are sometimes added as modifiers. This is used both for analytical (SFC) and extraction (SFE) purposes.
Techniques where DNA is delivered directly into organelles at high speed using projectiles coated with nucleic acid, shot from a helium-powered gun (gene gun). One of these techniques involves immunization by DNA VACCINES, which delivers DNA-coated gold beads to the epidermis.
White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
The giving of drugs, chemicals, or other substances by mouth.
The residual portion of BLOOD that is left after removal of BLOOD CELLS by CENTRIFUGATION without prior BLOOD COAGULATION.
Vesicles that are involved in shuttling cargo from the interior of the cell to the cell surface, from the cell surface to the interior, across the cell or around the cell to various locations.
Transparent, tasteless crystals found in nature as agate, amethyst, chalcedony, cristobalite, flint, sand, QUARTZ, and tridymite. The compound is insoluble in water or acids except hydrofluoric acid.
A disorder of HEMOSTASIS in which there is a tendency for the occurrence of THROMBOSIS.
Manufacturing technology for making microscopic devices in the micrometer range (typically 1-100 micrometers), such as integrated circuits or MEMS. The process usually involves replication and parallel fabrication of hundreds or millions of identical structures using various thin film deposition techniques and carried out in environmentally-controlled clean rooms.
Agents causing contraction of the pupil of the eye. Some sources use the term miotics only for the parasympathomimetics but any drug used to induce miosis is included here.
Inorganic or organic compounds containing trivalent iron.
The quality or state of being wettable or the degree to which something can be wet. This is also the ability of any solid surface to be wetted when in contact with a liquid whose surface tension is reduced so that the liquid spreads over the surface of the solid.
Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
The extent to which the active ingredient of a drug dosage form becomes available at the site of drug action or in a biological medium believed to reflect accessibility to a site of action.
Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)
An anionic surface-active agent used for its wetting properties in industry and used in medicine as an irritant and sclerosing agent for hemorrhoids and varicose veins.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Apparatus, devices, or supplies intended for one-time or temporary use.
Tree-like, highly branched, polymeric compounds. They grow three-dimensionally by the addition of shells of branched molecules to a central core. The overall globular shape and presence of cavities gives potential as drug carriers and CONTRAST AGENTS.
Microdevices that combine microfluidics technology with electrical and/or mechanical functions for analyzing very small fluid volumes. They consist of microchannels etched into substrates made of silicon, glass, or polymer using processes similar to photolithography. The test fluids in the channels can then interact with different elements such as electrodes, photodetectors, chemical sensors, pumps, and valves.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
A polyhedral CARBON structure composed of around 60-80 carbon atoms in pentagon and hexagon configuration. They are named after Buckminster Fuller because of structural resemblance to geodesic domes. Fullerenes can be made in high temperature such as arc discharge in an inert atmosphere.
A diuretic and renal diagnostic aid related to sorbitol. It has little significant energy value as it is largely eliminated from the body before any metabolism can take place. It can be used to treat oliguria associated with kidney failure or other manifestations of inadequate renal function and has been used for determination of glomerular filtration rate. Mannitol is also commonly used as a research tool in cell biological studies, usually to control osmolarity.
The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
The analysis of a chemical substance by inserting a sample into a carrier stream of reagent using a sample injection valve that propels the sample downstream where mixing occurs in a coiled tube, then passes into a flow-through detector and a recorder or other data handling device.
Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Endothelial cells that line venous vessels of the UMBILICAL CORD.
A phthalic indicator dye that appears yellow-green in normal tear film and bright green in a more alkaline medium such as the aqueous humor.
The engulfment and degradation of cells by other cells.
The chemical and physical integrity of a pharmaceutical product.
Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)
Colloids with liquid continuous phase and solid dispersed phase; the term is used loosely also for solid-in-gas (AEROSOLS) and other colloidal systems; water-insoluble drugs may be given as suspensions.
Methods of creating machines and devices.
Derivatives of ACETIC ACID which contain an hydroxy group attached to the methyl carbon.
An involuntary contraction of a muscle or group of muscles. Spasms may involve SKELETAL MUSCLE or SMOOTH MUSCLE.
Nanometer-scale wires made of materials that conduct electricity. They can be coated with molecules such as antibodies that will bind to proteins and other substances.
Established cell cultures that have the potential to propagate indefinitely.
Tablets coated with material that delays release of the medication until after they leave the stomach. (Dorland, 28th ed)
A non-steroidal anti-inflammatory agent with analgesic, anti-inflammatory, and antipyretic properties. It is an inhibitor of cyclooxygenase.
Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
The clear, viscous fluid secreted by the SYNOVIAL MEMBRANE. It contains mucin, albumin, fat, and mineral salts and serves to lubricate joints.
The coagulation of tissue by an intense beam of light, including laser (LASER COAGULATION). In the eye it is used in the treatment of retinal detachments, retinal holes, aneurysms, hemorrhages, and malignant and benign neoplasms. (Dictionary of Visual Science, 3d ed)

Tissue factor activity is increased in a combined platelet and microparticle sample from cancer patients. (1/383)


Microparticles are vectors of paradoxical information in vascular cells including the endothelium: role in health and diseases. (2/383)

Both inflammation and thrombosis can be orchestrated by the interactions between circulating cells, such as leukocytes and platelets, with vascular, endothelial and smooth muscle cells, which, during activation or apoptosis, can release circulating microparticles (MPs). Indeed, MPs are membrane vesicles with procoagulant and proinflammatory properties. MPs are present in blood from healthy individuals and in patients under several pathological states, for instance sepsis, preeclampsia, Crohn's disease and diabetes, strengthening the notion that MPs may play a role in these diseases. Circulating MPs or those generated in vitro from apoptotic T cells display deleterious effects on endothelial and/or vasomotor function. In contrast, MPs might be protective to endothelial cells. We have shown that MPs harboring the morphogen sonic hedgehog may represent a new therapeutic approach against endothelial dysfunction during acute severe endothelial injury. Indeed, these types of MPs induce NO release, decrease production of reactive oxygen species and induce angiogenesis from endothelial cells. This protective role for the endothelium was confirmed also by their in vivo injection in mice in which they were also able to reverse endothelial dysfunction in a model of heart ischemia/reperfusion. On the contrary, MPs from preeclamptic women compared to those from normal pregnant women showed pro-inflammatory properties in the vascular wall inducing vascular hyporeactivity in vessels from humans and mice. These effects were associated with complex interactions between NO and cyclooxygenase systems via endothelial cell activation. Altogether, these findings suggest that MPs can be considered as vectors of biological messages for vascular homeostasis, during immunity and inflammation.  (+info)

The role of cell death in the pathogenesis of autoimmune disease: HMGB1 and microparticles as intercellular mediators of inflammation. (3/383)


The relationship between plasma microparticles and disease manifestations in patients with systemic sclerosis. (4/383)


Measurement of platelet-derived microparticle levels in the chronic phase of cerebral infarction using an enzyme-linked immunosorbent assay. (5/383)

Assessment of platelet function is a critical component of the treatment and secondary prevention of cerebral infarction, and measurement of platelet-derived microparticle (PDMP) levels using flow cytometry may be a good indicator of platelet function. However, the flow cytometric analysis is not feasible in a variety of clinical situations. The goal of the present study was to measure PDMP levels using an enzyme-linked immunosorbent assay (ELISA) in chronic cerebral infarction patients and to determine the utility of PDMP level measurement for the monitoring of the effect of cilostazol and aspirin. A crossover study was performed using 4-weeks of aspirin (100 mg/day) and 4-weeks of cilostazol (200 mg/day) in 18 patients. PDMP levels were also measured in 20 volunteers as controls. Experiments demonstrated that PDMP levels were significantly higher in chronic cerebral infarction patients (median 8.8 U/ml, interquartile range 5.1-14.9 U/ml, n=18) than in controls (median 5.5 U/ml, interquartile range 5.0-8.2 U/ml, n=20) (P=0.047). PDMP levels did not decrease after therapy with either aspirin (median 10.9 U/ml, interquartile range 6.2-17.9 U/ml, n=12) or cilostazol (median 9.2 U/ml, interquartile range 6.1-14.3 U/ml, n=12) compared with baseline PDMP levels in the 12 patients who completed this trial (median 11.4 U/ml, interquartile range 5.2-23.7 U/ml, n=12). There were no significant differences in PDMP levels between aspirin and cilostazol (P=0.61). In conclusion, PDMP levels as measured by ELISA were increased in patients with chronic cerebral infarction regardless of the anti-platelet therapy. This methodology may be a useful strategy of assessing platelet function in chronic cerebral infarction patients.  (+info)

Megakaryocyte-derived microparticles: direct visualization and distinction from platelet-derived microparticles. (6/383)


Dynamics of circulating microparticles in liver transplant patients. (7/383)

BACKGROUND & AIMS: Microparticles are small membrane vesicles released from the cell plasma membrane, particularly in cell stress, apoptosis and altered cellular viability. Hepatocellular carcinoma (HCC) is a hypervascular neoplasm with high levels of apoptosis and necrosis. We investigated the levels of circulating microparticles of both tumor and endothelial origins in liver transplant patients with hepatitis C (HepC) cirrhosis with and without HCC and compared them with healthy people and patients with partial hepatectomy. METHODS: Using immunolabeling of microparticles of different origin and flow cytometry-based enumeration of microparticles, the levels of circulating microparticles were studied in 8 patients with HepC and 8 patients with both HepC and HCC before and within two weeks after the transplant. RESULTS: The initial levels of circulating microparticles were increased in patients with HepC and HCC as compared to patients with HepC alone. They were also increased in liver transplant patients as compared to patients with partial hepatectomy or healthy people. Levels of circulating microparticles were dynamically changing after the transplant, showing an initial increase with a subsequent decrease by the end of the second week after surgery. In some patients with a complicated clinical outcome, the levels of microparticles were continuously increasing after the surgery. CONCLUSION: The levels of circulating microparticles of endothelial and hepatic origin in liver transplant patients dynamically change after surgery and correlate with the clinical outcome. Perspectively, the levels of circulating microparticles may be used in clinical practice as a marker of the functional status of the transplanted liver.  (+info)

Diagnostic role of endothelial microparticles in vasculitis. (8/383)


After long controversy, it is now recognized that essentially all neurodegenerative diseases have inflammatory components [1]. Many other neurological disorders are exacerbated by inflammation, including the progressive impairment following stroke or traumatic brain injury (TBI). It has been known that levels of circulating cell-derived microparticles (MP) are generally increased in inflammatory states, making them useful as biomarkers. More recently, certain species of MP or exo- somes have been implicated as causative agents in neuroinflammation, to be reviewed.. L.L. Horstman, BS • W. Jy, PhD (*) • Y.S. Ahn, MD. University of Miami, Department of Medicine (Wallace H. Coulter Platelet Laboratory), 1600 NW 10TH Ave., Mail Code R36A, Miami, FL 33136, USA e-mail: This email address is being protected from spam bots, you need Javascript enabled to view it © Springer International Publishing AG 2017. A. Minagar, J.S. Alexander (eds.), Inflammatory Disorders of the Nervous System, Current ...
RATIONALE: Microparticles are cell-derived membrane vesicles, relevant to a range of biological responses and known to be elevated in cardiovascular disease. OBJECTIVE: To investigate microparticle release during cardiac stress and how this response differs in those with vascular disease. METHODS AND RESULTS: We measured a comprehensive panel of circulating cell-derived microparticles by a standardized flow cytometric protocol in 119 patients referred for stress echocardiography. Procoagulant, platelet, erythrocyte, and endothelial but not leukocyte, granulocyte, or monocyte-derived microparticles were elevated immediately after a standardized dobutamine stress echocardiogram and decreased after 1 hour. Twenty-five patients developed stress-induced wall motion abnormalities suggestive of myocardial ischemia. They had similar baseline microparticle levels to those who did not develop ischemia, but, interestingly, their microparticle levels did not change during stress. Furthermore, no stress-induced
0175]1. Hugel B, Martinez M C, Kunzelmann C, Freyssinet J M. Membrane microparticles: two sides of the coin. Physiology (Bethesda, Md. February 2005; 20:22-27. [0176]2. Mallat Z, Benamer H, Hugel B, Benessiano J, Steg P G, Freyssinet J M, Tedgui A. Elevated levels of shed membrane microparticles with procoagulant potential in the peripheral circulating blood of patients with acute coronary syndromes. Circulation. Feb. 29 2000; 101(8):841-843. [0177]3. Nieuwland R, Berckmans R J, Rotteveel-Eijkman R C, Maquelin K N, Roozendaal K J, Jansen P G, ten Have K, Eijsman L, Hack C E, Sturk A. Cell-derived microparticles generated in patients during cardiopulmonary bypass are highly procoagulant. Circulation. Nov. 18 1997; 96(10):3534-3541. [0178]4. Tesse A, Martinez M C, Hugel B, Chalupsky K, Muller C D, Meziani F, Mitolo-Chieppa D, Freyssinet J M, Andriantsitohaina R. Upregulation of proinflammatory proteins through NF-kappaB pathway by shed membrane microparticles results in vascular hyporeactivity. ...
TY - JOUR. T1 - Phenotypic assessment of endothelial microparticles in patients with heart failure and after heart transplantation. T2 - Switch from cell activation to apoptosis. AU - Garcia, Santiago. AU - Chirinos, Julio. AU - Jimenez, Javier. AU - Del Carpio Muñoz, Freddy. AU - Canoniero, Mariana. AU - Jy, Wenche. AU - Jimenez, Joaquin J. AU - Horstman, Lawrence. AU - Ahn, Yeon. PY - 2005/12/1. Y1 - 2005/12/1. N2 - Background: Endothelial microparticles (EMPs) are sub-microscopic membrane vesicles that are shed from the surface of endothelial cells during activation, injury and/or apoptosis. Endothelial cells release phenotypically and quantitatively distinct endothelial microparticles (EMPs) in activation and apoptosis. Therefore, the phenotypic assessment of EMPs can provide useful information reflecting the nature of endothelial injury. We tested the hypothesis that heart transplantation (HT) modifies the pattern of endothelial injury seen in patients with congestive heart failure (CHF). ...
Acute HIV-1 infection results in dysregulated immunity, which contributes to poor control of viral infection. DCs are key regulators of both adaptive and innate immune responses needed for controlling HIV-1, and we surmised that factors elicited during acute HIV-1 infection might impede DC function. We derived immature DCs from healthy donor peripheral blood monocytes and treated them with plasma from uninfected control donors and donors with acute HIV-1 infections. We found that the plasma from patients with HIV specifically inhibited DC function. This suppression was mediated by elevated apoptotic microparticles derived from dying cells during acute HIV-1 infection. Apoptotic microparticles bound to and inhibited DCs through the hyaluronate receptor CD44. These data suggest that targeting this CD44-mediated inhibition by apoptotic microparticles could be a novel strategy to potentiate DC activation of HIV-specific immunity.
Recurrent miscarriage is frustrating for the physician and a heartbreaking experience for the patient. Approximately 5% of couples trying to conceive have two consecutive miscarriages. Despite a thorough study of patients, the aetiology of this common obstetric complication is unknown in 50% of cases. Known causes include abnormal chromosomes, endocrinological disorders and uterine abnormalities. Although antiphospholipid antibodies have been demonstrated in miscarriages, the role played by alloimmune mechanisms remains unclear. New immunological approaches such as natural killer cells, regulatory T cells, tumour necrosis factor α, cell-derived microparticles, leptin, certain glycoproteins and cytokines should be considered. The management of thyroid diseases and immunological disorders is continuously evolving. Several genetic diagnostic procedures such as parental karyotyping and preimplantation genetic screening should probably not be used routinely. Antiphopholipid syndrome and some ...
TY - JOUR. T1 - Circulating endothelial microparticles as a measure of early lung destruction in cigarette smokers. AU - Gordon, Cynthia. AU - Gudi, Kirana. AU - Krause, Anja. AU - Sackrowitz, Rachel. AU - Harvey, Ben Gary. AU - Strulovici-Barel, Yael. AU - Mezey, Jason G.. AU - Crystal, Ronald. PY - 2011/7/15. Y1 - 2011/7/15. N2 - Rationale: There is increasing evidence thatemphysemais associated with primary loss of pulmonary capillary endothelium. Plasma levels of endothelial microparticles (EMPs), small vesicles released from activated or apoptotic endothelial cells, are elevated in vascularrelated disorders. Objectives: To evaluate whether plasma EMP levels are elevated in smokers with early lung destruction as assessed by normal spirometry but reduced diffusing capacity of the lung for carbon monoxide (DLCO). Methods: Lung health was assessed by pulmonary function tests (PFTs: spirometry, total lung capacity, DLCO) and chest X-ray; smoking status was assessed by urine nicotine and ...
TY - JOUR. T1 - C-reactive protein induces release of both endothelial microparticles and circulating endothelial cells in vitro and in vivo. T2 - Further evidence of endothelial dysfunction. AU - Devaraj, Sridevi. AU - Kumaresan, Pappanaicken R.. AU - Jialal, Ishwarlal. PY - 2011/12. Y1 - 2011/12. N2 - BACKGROUND: Inflammation is pivotal in atherosclerosis. A key early event in atherosclerosis is endothelial dysfunction. C-reactive protein (CRP), the prototypic marker of inflammation in humans, is a risk marker for cardiovascular disease, and there is mounting evidence to support its role in atherothrombosis. CRP has been shown to promote endothelial dysfunction both in vitro and in vivo. Emerging biomarkers of endothelial dysfunction include circulating endothelial cells (CECs) and endothelial microparticles (EMPs). However, there is a paucity of data examining the effect of CRP on CEC and EMP production in vitro and in vivo. METHODS: In this report, we treated human aortic endothelial cells ...
Circulating endothelial microparticles: a promising biomarker of acute kidney injury after cardiac surgery with cardiopulmonary bypass
The results of these studies demonstrate that calcineurin inhibitors cause endothelial cells to increase the number of microparticles released into the circulation from the cell surface. Calcineurin inhibitors also alter the composition of the microparticles such that they become complement activating. We found that alternative pathway-deficient mice are protected from CsA-induced renal and vascular injury. We also found that endothelial microparticles cause injury of unmanipulated endothelial cells in vitro, and they cause mesangial proliferation and complement activation when passively transferred into wild-type mice in vivo. These results demonstrate that CsA-induced endothelial microparticles can cause bystander injury of endothelial cells, and they promote glomerular complement activation and mesangial expansion. Furthermore, preliminary data from human transplant patients indicate that treatment of these patients with tacrolimus is also associated with generation of endothelial ...
Our study demonstrates that CRC cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells, suggesting that microvesicles of cancer cells can be involved in tumor growth and metastasis by facilitating angiogenesis-related processes. This infor …
Maturation (MP) or polishing ponds are widely used throughout the world as a final or tertiary wastewater treatment process for effluent quality improvement from secondary biological treatment systems, including FPs. MPs can be expected to reduce total suspended solids (TSS), ammonia, nitrate and phosphorus levels in effluent, but the primary function of MPs in most parts of the world is the removal of pathogenic organisms. A number of mechanisms in MPs are responsible for pathogen reduction, including settlement, natural die off (a function of time and temperature), elevated pH, UV radiation and predation. The design, size and number (usually in series) of MPs are typically based on the desired bacteriological quality of the final effluent. MPs are typically designed at the same depth as facultative ponds (2 to 5 feet (0.7 to 1.5 m)). Retention times in MPs are typically designed at 10 to 14 days for pathogen reduction; however, shorter retention times are required for TSS removal (four to five ...
Microparticles are membrane vesicles with procoagulant and proinflammatory properties released during cell activation or apoptosis. Microparticles from monocytes have been implicated in atherosclerosis and vascular inflammation, but their direct effects on endothelial cells are not completely elucidated. The present study was designed to dissect the signaling pathways of monocytic microparticles in endothelial cells with respect to both NO pathway and reactive oxygen species. Microparticles were produced by treatment of human monocytic cell line THP-1 with the apoptotic agent VP-16. Human endothelial cells were treated with monocytic microparticles and then, we studied their effects on nitrosative and oxidative stresses. Incubation of human endothelial cells with microparticles enhanced the production of NO without affecting superoxide anions generation. Microparticles did not affect endothelial NO synthase expression and its phosphorylation. Interestingly, microparticles decreased caveolin-1 ...
Acute lung injury (ALI) results from infectious challenges and from pathologic lung distention produced by excessive tidal volume delivered during mechanical ventilation (ventilator-induced lung injury [VILI]) and is characterized by extensive alveolar and vascular dysfunction. Identification of nov …
Microparticles are a heterogeneous population of membrane-coated vesicles which can be released from virtually all cell types during activation or apoptosis. Release occurs from the cell surface in an exogenous budding process involving local rearrangement of the cytoskeleton. Given their origin, these particles can be identified by staining for cell surface markers and annexin V. As shown in in vitro studies, microparticles may represent a novel subcellular element for intercellular communication in inflammation. Thus, microparticles can transfer chemokine receptors and arachidonic acid between cells, activate complement, promote leukocyte rolling and stimulate the release of pro-inflammatory mediators. Under certain conditions, however, microparticles may also exert anti-inflammatory properties by inducing immune cell apoptosis and the production of anti-inflammatory mediators. Microparticles may play an important role in the pathogenesis of rheumatologic diseases as evidenced by their elevation in
Simoncini S, Njock MS, Robert S, Camoin-Jau L, Sampol J, Harlé JR, Nguyen C, Dignat-George F, Anfosso F. Circ Res. 2009 Apr 24 ;104 (8):943-51 (...)
Oxidative Medicine and Cellular Longevity is a unique peer-reviewed, Open Access journal that publishes original research and review articles dealing with the cellular and molecular mechanisms of oxidative stress in the nervous system and related organ systems in relation to aging, immune function, vascular biology, metabolism, cellular survival and cellular longevity. Oxidative stress impacts almost all acute and chronic progressive disorders and on a cellular basis is intimately linked to aging, cardiovascular disease, cancer, immune function, metabolism and neurodegeneration. The journal fills a significant void in todays scientific literature and serves as an international forum for the scientific community worldwide to translate pioneering
Microparticles (MPs) are fragments of cell membranes released from stimulated or apoptotic cells that have long been considered innocent remnants of cell destruction. Evidence is accumulating, however, that the circulating and tissue-derived MPs may elicit a strong thrombogenic and inflammatory response (1). In addition to MPs, activated cells can also release smaller vesicle-denominated exosomes that are relatively unexplored in the research area of vascular biology (2).. Circulating MPs are being explored as a potential source for biomarker discovery. Platelet MPs have been associated with clinically evident atherosclerotic disease in diabetes type II patients (3), and leukocyte-derived MPs are predictive of subclinical atherosclerosis in asymptomatic patients (4).. Experimental and clinical data consistently point to a causal role for MPs in atherosclerosis development and progression. Recent reports revealed their contribution to hemostatic and inflammatory responses, vascular remodeling and ...
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To study the role of microparticles in causing a major complication of malaria infection, namely cerebral malaria. - Research Supervisor Connect - University of Sydney, Australia
Background: Early and non-invasive biomarkers of kidney damage are needed to identify hypertensive patients at risk for kidney damage. Urinary micropaticles (UMPs) have gained significant attention as potential novel biomarkers for kidney damage, and have already been identified in pre-albuminuric diabetic glomerular injury. These vesicles are less than 1 micron in size and carry markers of the parent cell. We hypothesized that podocyte derived UMPs are elevated in angiotensin II-induced hypertension (HTN). Methods: Primary podocytes were isolated and grown in culture. Wild-type mice were treated with AII (400ng/kg/min) via mini-osmotic pumps. Untreated WT mice served as controls. 24 hour urines were collected after 5 days of AII treatment. Enumeration and phenotyping of MPs was done of podocyte culture supernatant and urine. Podocalyxin (Pcal), podoplanin (Ppla) and annexin 5 (AV) were used as surface markers.. Result: Pcal and Ppla positive MPs as well as AV positive and negative MPs were ...
CA153 : The instrument used is the Roche Cobas. The Roche cancer antigen 15-3 (CA 15-3) method is a sandwich electrochemiluminescence immunoassay that employs a biotinylated monoclonal CA 15-3-specific antibody and a monoclonal CA 15-3-specific antibody. CA 15-3 in the automatically prediluted specimen reacts with both the biotinylated monoclonal CA 15-3-specific antibody (mouse) and the monoclonal CA 15-3-specific antibody (mouse) labeled with a ruthenium complex, forming a sandwich complex. Streptavidin-coated microparticles are added and the mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell. Application of voltage to the electrode induces the chemiluminescent emission, which is then measured.(Package insert: Roche CA 15-3 reagent, Roche Diagnostic Corp., Indianapolis, IN 2010-09, V14)
BOSTON, Massachusetts (PNN) - March 28, 2017 - A team of scientists at the Boston Childrens Hospital have invented what is being considered one the greatest medical breakthroughs in recent years. They have designed a microparticle that can be injected into a persons bloodstream that can quickly oxygenate his or her blood. This will even work if the ability to breathe has been restricted, or even cut off entirely.. This finding has the potential to save millions of lives every year. The microparticles can keep an object alive for up to 30 minutes after respiratory failure. This is accomplished through an injection into the patients veins. Once injected, the microparticles can oxygenate the blood to near normal levels. This has countless potential uses as it allows life to continue when oxygen is needed but unavailable. For medical personnel, this is just enough time to avoid risking a heart attack or permanent brain injury when oxygen is restricted or cut off to patient.. Dr. John Kheir, who ...
2015. Ghelani H, Razmovski-Naumovski V, Kyada AV, Parekh HS, Li GQ, Nammi S. Myoglobin and low density lipoprotein glycation in cardiac complications of diabetes: Current perspectives and therapeutic options. Indian J Med Res. 2015. In Press. Jadeja R, Devkar RV, Nammi S. Hepatoprotective potential of herbal medicine. Evid-Based Compl Alt. [Editorial]. 2015;2015.. Kam A, Li KM, Razmovski-Naumovski V, Nammi S, Chan K, Grau GE, et al. Curcumin reduces tumour necrosis factor-enhanced annexin V-positive microparticle release in human vascular endothelial cells. J Pharm Pharm Sci. 2015;18(4):424-33.. Thounaojam MC, Nammi S, Jadeja R. Natural products for the treatment of obesity, metabolic syndrome, and type 2 diabetes 2014. Evid-Based Compl Alt. [Editorial]. 2015;2015. DOI: 10.1155/2015/392681.. Yang WC, Chang CLT, Li CR, Nammi S, Cho WCS. Complementary and alternative medicine for diabetes 2014. Evid-Based Compl Alt. [Editorial]. 2015;2015. DOI: 10.1155/2015/685248.. 2014. Li Y, Tran VH, Kota BP, ...
TY - JOUR. T1 - Modification of molecular conformation of spray-dried whey protein microparticles improving digestibility and release characteristics. AU - Ye, Qianyu. AU - Woo, Meng Wai. AU - Selomulya, Cordelia. PY - 2019/5/15. Y1 - 2019/5/15. N2 - This study reports on the preparation of riboflavin-loaded whey protein isolate (WPI) microparticles, using desolvation and then spray drying. Ethanol desolvation led to the exposure of embedded hydrophobic amino acids of WPI to riboflavin, facilitating the formation of riboflavin-WPI complexes. The extent of desolvation and cross-linking influenced the morphology of the spray-dried microparticles, while the moisture content of microparticles decreased with desolvation and increased with crosslinking. The modification of WPI conformation upon desolvation could be retained in the dry state via spray drying. The gastric resistance, release site and release characteristics of microparticles were readily adjusted by varying the ethanol and calcium ion ...
In the study reported in this paper, we characterized anisotropic van der Waals interactions programmed into microparticles via control of internal LC ordering by quantifying kinetically controlled colloid adsorption over the surfaces of the LC microparticles. Our experiments and supporting calculations revealed that spatial variation of the van der Waals interactions across the surfaces of the LC microparticles was as large as 20 kBT, a magnitude that is sufficiently large to be useful in engineering the bottom-up assembly of soft materials (18). We note that the LC microparticles used in our experiments were prepared by polymerization of RM257. The polymer network formed by the RM257 prevented lateral motion of probe colloids that adsorbed to the surfaces of the LC microparticles. In contrast, a number of past studies have reported adsorption of colloids onto the surfaces of LC microdroplets with mobile interfaces (23, 30). When the interfaces of the LC microdroplets are mobile, adsorbed ...
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Overall research in my lab explores the molecular mechanisms of cellular signal transduction, with several related foci: 1) intercellular signaling in platelet-tumor interactions, including the contributions of Ras family small G proteins; 2) the contributions of protein trafficking to the signaling and oncogenic functions of Ras proteins; 3) Ras signaling in platelets, regulating thrombosis and hemostasis. My long term goal is to reach a new understanding of cellular information processing and its contributions to signal responses and to translate these findings into pre-clinical approaches.. 1. Intercellular signaling in platelet-tumor interactions.. We recently discovered that platelet microparticles - small extracellular vesicles released by blood platelets into plasma - infiltrate solid tumors by means of the unusual permeability of tumor vasculature, whereupon the platelet microparticles anchor to tumor cells and transfer genetic information in the form of platelet-derived microRNAs ...
55% carbohydrates), with endothelial function measurement repeated after 1 year. As secondary objectives and to explore different underlying mechanisms in the modulation of endothelial function, we quantified endothelial microparticles (EMPs) and endothelial progenitor cells (EPCs) and evaluated, in 24 preselected patients, in vitro cellular processes related to endothelial damage (reactive oxygen species, apoptosis, and senescence) and endothelial repair (cell proliferation and angiogenesis), as well as other modulators (micro-RNAs [miRNAs] and proteins). Patients who followed the Mediterranean diet had higher FMD (3.83%; 95% confidence interval [CI]: 2.91-4.23) compared with those in the low-fat diet (1.16%; 95% CI: 0.80 to 1.98) with a difference between diets of 2.63% (95% CI: 1.89-3.40, p = 0.011), even in those patients with severe endothelial dysfunction. We observed higher EPC levels (group difference: 1.64%; 95% CI: 0.79-2.13, p = 0.028) and lower EMPs (group difference: -755 EMPs/µl; ...
VEGF/VEGFR inhibitors, used as anti-angiogenic drugs to treat cancer, induce severe hypertension. Molecular mechanisms are unclear, but nitric oxide (NO) and oxidative stress may be involved. We questioned whether reactive oxygen species (ROS) and Ang II also play a role in VEGF inhibitor-induced vascular dysfunction. Human microvascular endothelial cells (HMECs) were stimulated with vatalanib (VAT-VEGFR inhibitor) and gefitinib (GEF-EGFR inhibitor) in the absence/presence of Ang II. Activation of eNOS and MAPKs were assessed by immunoblotting. Antioxidant enzyme mRNA was analysed by qPCR. Endothelial microparticles, biomarkers of endothelial damage, tend to increase in subjects treated with VEGFR inhibitors. Phosphorylation of eNOS (28.3% ± 7.1) was decreased by VAT (p , 0.05). VAT decreased mRNA expression of Nox4 (0.5 ± 0.2) and H2O2-regulating antioxidants enzymes such as catalase (0.4 ± 0.1) and glutathione peroxidase (0.4 ± 0.1), while increased mRNA levels of Nox5 (3.35 ± 1.1) (p , ...
Background: Neopetrosiamide A (NeoA) is a 28-amino acid tricyclic peptide originally isolated from a marine sponge as a tumor cell invasion inhibitor whose mechanism of action is unknown. Methodology/Principal Findings: We show that NeoA reversibly inhibits tumor cell adhesion, disassembles focal adhesions in pre-attached cells, and decreases the level of beta 1 integrin subunits on the cell surface. NeoA also induces the formation of dynamic, membrane-bound protrusions on the surface of treated cells and the release of membrane-bound vesicles into the culture medium. Proteomic analysis indicates that the vesicles contain EGF and transferrin receptors as well as a number of proteins involved in adhesion and migration including: beta 1 integrin and numerous alpha integrin subunits; actin and actin-binding proteins such as cofilin, moesin and myosin 1C; and membrane modulating eps15 homology domain (EHD) proteins. Surface labeling, trafficking inhibition, and real-time imaging experiments all ...
Supplementary MaterialsSupplemental Shape 1: Verification of endothelial cell apoptosis by TUNEL-staining. HVT (cohens = 0.85)] post-exercise in comparison to pre-values, while HIT revealed only small to moderate adjustments on c-miRs-21 (cohens = ?0.28) and c-miR-126 (cohens = 0.53). c-miR-16 was just slightly suffering from SIT (1.4-fold; cohens = 0.57), HVT (1.3-fold; cohens = 0.61) or Strike (1.1-fold; cohens = 0.2). Additional experiments revealed that miR-126 and miR-21 are of endothelial origin mainly. Importantly, under circumstances of endothelial apoptosis, miR-126 and miR-21 are loaded from endothelial cells into endothelial microparticles, that have been proven to transfer miR-126 into focus on vascular smooth muscle tissue cells. Summary: Taken collectively, we discovered that SIT and HVT are from the launch of endothelial miRNAs in to the blood flow, that may work as intercellular conversation products regulating vascular biology. miR-39 serum/plasma spike-in control (cel-39; ...
M. ABBAS, L. JESEL, C. AUGER, L. AMOURA, N. MESSAS, G. MANIN, C. RUMIG, A.J. LEON-GONZALEZ, T.P. RIBEIRO, G.C. SILVA, R. ABOU-MERHI, E. HAMADE, M. HECKER, Y. GEORG, N. CHAKFE, P. OHLMANN, V.B. SCHINI-KERTH, F. TOTI & O. MOREL. Endothelial microparticles from acute coronary syndrome patients induce premature coronary artery endothelial cells ageing and thrombogenicity: role of the Ang II/AT1 receptor/NADPH oxidase-mediated activation of MAPKs and PI3-kinase pathways. Circulation 135, 280-296, 2017 - IF 17.20 - ...
Pitha J, Dorazilova Z, Melenovsky V, Kralova Lesna I, Stavek P, Stepankova J, Urban M, Maly J, Netuka I. The impact of left ventricle assist device on circulating endothelial microparticles - pilot study. Neuro Endocrinol Lett. 2012 Jan; 33(Suppl 2): 68-72 ...
A beam of accelerated microparticles of substantially uniform velocity is passed transversely through an intense pulsed laser beam. In traversing the laser beam, the microparticles are thermally evapo
TGF-β3 is enzymatically immobilized by transglutaminase-2 action to poly(L-lactic acid) microparticles coated with collagen II. Microparticles are then encapsulated with stem cells inside liquified spherical compartments enfolded with a permselective shell through layer-by-layer adsorption. Magnetic nanoparticles are electrostatically bound to the multilayered shell, conferring magnetic-response ability. The goal of this study is to engineer a closed environment inside which encapsulated stem cells would undergo a self-regulated chondrogenesis. To test this hypothesis, capsules are cultured in chondrogenic differentiation medium without TGF-β3. Their biological outcome is compared with capsules encapsulating microparticles without TGF-β3 immobilization and cultured in normal chondrogenic differentiation medium containing soluble TGF-β3. Glycosaminoglycans quantification demosntrates that similar chondrogenesis levels are achieved. Moreover, collagen fibrils resembling the native ...
Summary of Facts and Submissions. I. This appeal is from the interlocutory decision of the Opposition Division concerning maintenance of European patent No. 1 328 683 in amended form on the basis of the then pending main request. The independent Claim 1 reads:. 1. A process for making paper or paper board comprising forming a cellulosic suspension, flocculating the suspension, draining the suspension on a screen to form a sheet and then drying the sheet,. characterised in that. the suspension is flocculated using a flocculating system comprising a siliceous material and organic microparticles which have an unswollen particle diameter of less than 750 nanometers,. wherein a further flocculating material is included into the cellulosic suspension before adding the polymeric microparticles and siliceous material,. and in which the flocculating material is cationic and is a natural or synthetic polymer,. in which the microparticles are made from anionic copolymers comprising from 0 to 99 parts, by ...
The disassembly of apoptotic cells into small membrane-bound vesicles termed apoptotic bodies (ApoBDs) is a hallmark of apoptosis; however, the functi
A novel microparticle preparation comprising microparticles of a polymer which contain a drug and are coated with a film of an agent for preventing aggregation of the microparticles is disclosed. The preparation is produced by spraying a solution of a polymer containing a drug and an aqueous solution of an agent for preventing aggregation of the microparticles separately from different nozzles and contacting them with each other in a spray dryer.
Methods: The measles antigen was incorporated into the biodegradable, crosslinked-albumin matrix and spray dried using Buchi mini spray dryer B-290 to formulate the vaccine loaded microparticles. The microparticles were characterized for size, charge, and polydispersity index (PDI). The surface morphology of microparticles was visualized by Scanning Electron Microscopy. The induction of an immune response by the microparticulate vaccine was confirmed via spectroscopic Griesss assay. The expression of antigen-presenting molecules, MHC I and MHC II, and their co-stimulatory molecules CD80 and CD40 was assessed on the surface of dendritic cells using BD Accuri C6 plus flow cytometer. The equivalent amount of blank microparticles (without antigen and adjuvant) was used as control. The cytotoxicity of microparticles was assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. The uptake of microparticles by APCs was studied as a function of time. The in vivo efficacy of ...
The argument that we must do everything a Minister demands because he has been democratically chosen does not stand up to close inspection. MPs are not chosen by the people - they are chosen by their local constituency parties: thirty-five men in grubby raincoats or thirty-five women in silly hats. The further selection process is equally a nonsense: there are only 630 MPs and a party with just over 300 MPs forms a government and of these 300, 100 are too old and too silly to be ministers and 100 too young and too callow. Therefore there are about 100 MPs to fill 100 government posts. Effectively no choice at all ...
The intensive use of plastics and derivatives during the last century has increased the contamination of animal habitats. The breakdown of these primary plastics in the environment results in microplastics (MP), small fragments of plastic typically <1-5 mm in size. Apart from the potential negative effects of the MPs per se, it is generally assumed that microplastics may increase the exposure of marine aquatic organisms to chemicals associated with the plastics. In addition, to enhance the performance of plastics, additives are added during manufacture. Furthermore, they are active in absorbing other contaminants and be used as vectors of highly and well‐documented persistent contaminants. Finally, these small MPs are easily ingested by animals and affect their physiology and behaviour. Thus, aquatic living organisms are continuously exposed to these MPs, and associated contaminants, and could suffer from its contamination but also introduce them into the food chain.
Chepkwony said that they wanted the structure of the National Parliament replicated in county assemblies so that if MPs are appointed as Ministers then MCAs are also appointed as CECs.. In the coming months we shall hold an AGM for all MCAs and we shall combine our recommendations and hand them to the BBI taskforce, he said.. On his part, the forum chairman Ndegwa Wahome said that they were against the proposal for regional governments noting that Kenyans were currently over-represented.. Wahome who is also the Nyandarua Assembly Speaker, said that the current system should be strengthened adding that wards should be funded as one way of addressing the challenges facing Wanjiku.. ...
Thirty years for The Three Profiteers and the Parasite Peer : John Wright a writer and specialist in crime is outraged along with millions of other people that only four MPs are to be charged out of the hundreds who fiddled their expenses and stole public money John reckons we should give them all thirty y
Hospital parking charges have been slammed for placing an unfair financial burden on carers. MPs are looking to change the law to force health bosses to ex...
This petition has received 10,759 signatures. our MPS are dragging there feet on this . i have a letter from boris johnston who wants to bring in new regulations o...
A doctor who received a mystery package for Sir Bradley Wiggins has no record of his medical treatment at the time, MPs are told.
POSITEK 4G Microparticle Technology is the latest generation in Nalco microparticle innovation. This new programme is designed to promote drainage and retention without causing any negative effects caused by over-flocculation. | Ecolab
Vesicles are small membrane-bound structures containing materials for export. They combine with the outer cell membrane, releasing their contents. Epithelial cells form lining layers. ...
University of Delaware, Doctoral Student, Chemical Engineering, 2013 - present. National Taiwan University, B.S., Chemical Engineering, 2008 - 2012. Research Project. Cell-derived microparticles (MPs) released from budding off the cell membrane of several type of mammalian cells. These MPs have been found playing an important role in cell-cell interaction by transffering RNA, protein and lipids. Based on the characteristics that these MP are target specific, biocompatible and immunologically inert, my project focus on engineering megakaryocyte-derived MPs as vesicles for exogenous-material-transfer, and investigating the key factors that manipulate the interactions between megakaryocyte-derived MPs with hematopoietic stem cell.. Technical Skills. ...
Extracellular vesicles are a heterogeneous group of membrane-enclosed vesicles, which play an important role in intercellular communication. Increasing number of studies have shown that tumor-derived extracellular vesicles might be involved in the transfer of oncogenic cargo (proteins, lipids, messenger RNA, microRNA, non-coding RNAs and DNA) through which cancer cells could shape the tumor microenvironment and influence tumor progression. Nasopharyngeal carcinoma-derived extracellular vesicles have also reported to facilitate tumor proliferation, metastasis and immune escape. Moreover, nasopharyngeal carcinoma-derived extracellular vesicles might serve as biomarkers for early diagnosis and therapeutic targets. The present review provides information on the biological and clinical significance of extracellular vesicles in tumors, especially in nasopharyngeal carcinoma ...
LRP-mediated agLDL uptake has proved to be one of the main mechanisms for intracellular lipid accumulation in human VSMCs,17-20 cells that have the capacity to secrete TF MP.22,27 In the present study, we hypothesized that agLDLs were able to induce cellular TF expression and activity. We found that both nLDL and agLDL induced TF mRNA after 2 hours of LDL exposure to the same level, in agreement with previous studies showing that nLDL and oxidized LDL induce TF mRNA in different cell types,12-14 which is likely unrelated to the receptor involved in LDL uptake. The increase in mRNA transcription levels induced by nLDL or agLDL did not require new protein synthesis but required a functional transcriptional mechanism. The increase in mRNA led to a strong increase in membrane-associated cellular TF protein expression. With regard to TF activity, the effect of agLDL on TF PCA, in contrast to a shorter time effect of nLDL, was maintained for 24 hours. The ability of nLDL to induce TF expression but ...
Background: Body fluids contain cell-derived vesicles ranging from 30 nm to 1 μm in diameter. The function, origin, and composition of these vesicles is disease dependent and therefore vesicles contain clinical information. The most common method to detect vesicles is flow cytometry, which guides vesicles through a laser beam in a hydrodynamically focused fluid stream. The unknown relationship between the measured light scattering intensity and the vesicle diameter resulted in unexplained contradictions between expected and observed results.. Methods: We combined light scattering measurements of polystyrene and silica beads with Mie calculations using an estimated refractive index of vesicles.. Results: We established the relationship between measured light scattering and the diameter of vesicles. We show that common gating strategies based on beads select vesicles (and cells) ranging from 800 to 2400 nm in diameter. For our flow cytometer, the smallest detectable silica beads were 204 nm, ...
Scientists report that adult cells reprogrammed to become primitive stem cells, called induced pluripotent stem cells (iPSCs), make more extracellular vesicles than other kinds of adult stem cells commonly used for this purpose in research. Extracellular vesicles are naturally abundant in many types of cells, which use the cargo-containing spheres to communicate with other cells. They are about one one-hundredth the diameter of a cell and can carry anything from fats and proteins to nucleic acids. When a cell releases an extracellular vesicle, other cells nearby slurp up the tiny packet and its contents, making it an attractive target for packaging treatments for diseased cells that are deteriorating or aging prematurely. To package a potential treatment in an extracellular vesicle, scientists typically use a cell called a mesenchymal stem cell, which is found among fat or bone marrow cells and gives rise to other fat and bone cells. Scientists genetically modify the stem cell to produce ...
This Research Topic addresses the emerging role of extracellular vesicles in cellular communications and immune responses. We will emphasize their use as biomarkers (research tool to better understand extracellular vesicle functions) and immune modulators (therapeutic tool to suppress or enhance immune responses). Extracellular Vesicles (EVs) are released in response to signals activating their parental cells. They are divided in two categories: small EVs (e.g. exosomes) and large EVs (e.g. microvesicles/ectosomes/microparticles). Small and large EVs differ in terms of size, release and budding mechanism, lipid and protein composition, and surface antigens. Research findings from the last decade suggest that EVs have an important, yet not completely understood, role in cell-cell communication. They can influence neighboring cells, act in an autocrine or paracrine manner, affect tumor growth, de novo-angiogenesis, and immunological functions. EVs are a novel and important tool to impact cell function and
TY - JOUR. T1 - Procoagulant activity, but not number, of microparticles increases with age and in individuals after a single venous thromboembolism. AU - Owen, B. A.L.. AU - Xue, A.. AU - Heit, J. A.. AU - Owen, W. G.. N1 - Funding Information: This work was supported by grants HL83141 (Heit, J.A. and Owen, W.G.) and HL90639 (Heit, J.A. and Owen, W.G.) from the National Heart Lung and Blood Institute. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.. PY - 2011/1. Y1 - 2011/1. N2 - The Calibrated Automated Thrombogram (CAT), a plate-based assay that measures thrombin generation and inhibition in plasma samples, is modified to measure the procoagulant activity of phospholipid associated with plasma microparticles (MP). The assay uses a tissue factor trigger without addition of 4 μM exogenous phospholipid (PL) used in the standard CAT. Calibrated with 4:1 posphatidylcholine- phosphatidylserine (PCPS) liposomes, the assay defines a median of 40 nM procoagulant phospholipid (PPL) ...
STUDY QUESTION Are circulating microparticles (MPs) altered in young women with polycystic ovary syndrome (PCOS)? SUMMARY ANSWER Women with PCOS have elevated concentrations of circulating platelet-derived MPs, which exhibit increased annexin V binding and altered microRNA (miR) profiles compared with healthy volunteers. WHAT IS KNOWN ALREADY Some studies have shown that cardiovascular risk is increased in young women with PCOS but the mechanisms by which this occurs are uncertain. Circulating MPs are elevated in patients with cardiovascular disease but the characteristics of MPs in patients with PCOS are unclear. STUDY DESIGN, SIZE, DURATION Case-control study comprising 17 women with PCOS (mean ± SD; age 31 ± 7 years, BMI 29 ± 6 kg/m2) and 18 healthy volunteers (age 31 ± 6 years, BMI 30 ± 6 kg/m2). PARTICIPANTS/MATERIALS, SETTING, METHODS The study was conducted in a University hospital. Nanoparticle tracking analysis (NTA) and flow cytometry (CD41 platelet, CD11b monocyte, CD144 ...
Title:Calcium-calpain Dependent Pathways Regulate Vesiculation in Malignant Breast Cells. VOLUME: 17 ISSUE: 5. Author(s):Jack Taylor, Ritu Jaiswal and Mary Bebawy*. Affiliation:Discipline of Pharmacy, Graduate School of Health, The University of Technology Sydney, NSW, Discipline of Pharmacy, Graduate School of Health, The University of Technology Sydney, NSW, Discipline of Pharmacy, Graduate School of Health, The University of Technology Sydney, P.O. Box: 123 Broadway, NSW 2007. Keywords:Atomic force microscopy, breast cancer, calcium, calpain, extracellular vesicles, microparticles, multidrug resistance, vesiculation.. Abstract:Background: Multidrug resistance in cancer (MDR) occurs when tumours become crossresistant to a range of different anticancer agents. One mechanism by which MDR can be acquired is through cell to cell communication pathways. Membrane-derived microparticles (MPs) are emerging as important signaling molecules in this process. MPs are released from most eukaryotic cells ...
Learn Basics of Extracellular Vesicles from Калифорнийский университет в Ирвайне. This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, ...
The duration of cisplatin release from most of the drug delivery devices seemed to be shorter than 14 days except large microparticles. The objective of this study was to fabricate and characterize cisplatin-loaded PLA microparticles, PLA/PLGA (30/70) composite microparticles, and fibers as formulations for long-term sustained delivery of cisplatin to treat C6 glioma in vitro by electrospray and electrospinning techniques. Cisplatin-loaded biodegradable microparticles with particle size of around 5 μm and fiber fabrics with diameter of 0.5-1.7 μm were obtained using electrospray and electrospinning techniques. Encapsulation efficiency and in vitro release of formulations were measured by ICP-OES. The encapsulation efficiency for different samples of microparticles was approximately from 33% to 72% and the fiber fabrics had encapsulation efficiency greater than 90%. Cisplatin-loaded microparticles showed typical characteristics of cisplatin release profile: a large initial burst followed by a ...
TY - JOUR. T1 - Ultra-Sensitive Automated Profiling of EpCAM Expression on Tumor-Derived Extracellular Vesicles. AU - Amrollahi, Pouya. AU - Rodrigues, Meryl. AU - Lyon, Christopher J.. AU - Goel, Ajay. AU - Han, Haiyong. AU - Hu, Tony Y.. PY - 2019/12/17. Y1 - 2019/12/17. N2 - Extracellular vesicles (EVs) are abundant in most biological fluids and considered promising biomarker candidates, but the development of EV biomarker assays is hindered, in part, by their requirement for prior EV purification and the lack of standardized and reproducible EV isolation methods. We now describe a far-field nanoplasmon-enhanced scattering (FF-nPES) assay for the isolation-free characterization of EVs present in small volumes of serum (, 5 µl). In this approach, EVs are captured with a cancer-selective antibody, hybridized with gold nanorods conjugated with an antibody to the EV surface protein CD9, and quantified by their ability to scatter light when analyzed using a fully automated dark-field microscope ...
Pulmonary stenosis (PS) is a congenital heart disease characterized by a dynamic or fixed anatomic obstruction of blood flow from the right ventricle to the pulmonary arterial vasculature. In the present study, extracellular vesicle long RNAs (EVLRs) from pregnant females who had healthy infants or PS infants were analyzed by RNA sequencing, and their diagnostic potential for PS during pregnancy was evaluated. A method for the selection of genes that could be considered as informative for the prediction PS based on extracellular vesicles (EVs) from pregnant females using long‑read RNA sequencing was developed. Blood samples were collected from females carrying fetuses with PS and females carrying unaffected fetuses (n=6 in each group). Physical characterization of EVs was performed using nanoparticle tracking analysis, transmission electron microscopy and western blotting. EVLRs from plasma were profiled by RNA sequencing and mRNA co‑expression modules were constructed by weighted gene ...
Recent studies suggest that extracellular vesicles may be the key to timely diagnosis and monitoring of genito-urological malignancies. In this study researchers investigated the composition and content of extracellular vesicles found in the urine of healthy donors and prostate cancer patients. Urine of 14 PCa patients
Title:Autophagy Driven Extracellular Vesicles in the Leukaemic Microenvironment. VOLUME: 20 ISSUE: 7. Author(s):Rebecca H. Horton, Tom Wileman and Stuart A. Rushworth*. Affiliation:Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich, NR4 7UQ, Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich, NR4 7UQ, Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich, NR4 7UQ. Keywords:Leukaemia, autophagy, extracellular vesicles, therapeutic, tumour microenvironment, autoimmunity.. Abstract:The leukaemias are a heterogeneous group of blood cancers, which together, caused 310,000 deaths in 2016. Despite significant research into their biology and therapeutics, leukaemia is predicted to account for an increased 470,000 deaths in 2040. Many subtypes remain without targeted therapy, and therefore the mainstay of treatment remains generic cytotoxic drugs with bone marrow transplant the sole definitive option. In this ...
The EasySep™ Human Extracellular Vesicle (CD63) Positive Selection Kit is designed to isolate CD63+ extracellular vesicles from biofluids in less than 30 minutes.
Macrophages are polarized into functional classically activated and alternatively activated (M2) phenotypes depending on their microenvironment, and these cells play an important role in the immune system. M2-like polarization of tumor-associated macrophages (TAMs) is activated by various secretions from cancer cells; however, the interaction between cancer cells and TAMs is not well understood. Recent studies showed that cancer cell-derived extracellular vesicles (EVs) contribute to tumor development and modulation of the tumor microenvironment. In the current study, we investigated colorectal cancer-derived EVs containing miR-145 with respect to the polarization of TAMs. Colorectal cancer cells positively secreted miR-145 via EVs, which were taken up by macrophage-like cells. Interestingly, colorectal cancer-derived EVs polarized macrophage-like cells into the M2-like phenotype through the downregulation of histone deacetylase 11. An in vivo study showed that EV-treated macrophages caused ...
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ThermoFisher Microparticles are manufactured in ISO-9001 certified, FDA-registered facilities following a computer-aided design. A wide range of microparticles of a variety of formats are available for use in diagnostics, research and industrial applications such as. ...
Mucopolysaccharidoses (MPS) are lysosomal storage disorders characterized by progressive accumulation of glycosaminoglycans (GAGs) due to deficiency of specific lysosomal enzymes. MPS are classified according to the enzyme ...
Extracellular vesicles (EVs) are specifically packed with nucleic acids, lipids, and proteins from their parental cell. subpopulation gun. In assessment to healthful plasma vesicles, most cancers plasma vesicles demonstrated modified indicators for platelet guns, suggesting buy NMS-1286937 a transformed vesicle release or proteins launching of EVs by platelets and a lower Compact disc8 transmission that might become connected with a reduced activity of NK cells or Capital t cells. As we barely recognized melanoma-derived vesicles in individuals plasma, we came to the conclusion that bloodstream cells caused the noticed variations. In overview, our outcomes query a immediate impact of most cancers cells on the structure of EVs in most cancers plasma, but rather claim for an roundabout impact of most cancers cells on the vesicle release or vesicle proteins launching by bloodstream cells. for 10?minutes (23). To deplete leukocytes and erythrocytes the platelet-rich plasma was centrifuged at ...
Extracellular vesicles (EVs), nanoparticles originated from different cell types, seem to be implicated in several cellular activities. In the Central Nervous System (CNS), glia and neurons secrete EVs and recent studies have demonstrated that the intercellular communication mediated by EVs has versatile functional impact in the cerebral homeostasis. This essential role may be due to their proteins and RNAs cargo that possibly modify the phenotypes of the targeted cells. Despite the increasing importance of EVs, little is known about their fluctuations in physiological as well as in pathological conditions. Furthermore, only few studies have investigated the contents of contemporary EVs subgroups (microvesicles, MVs and exosomes, EXOs) with the purpose of discriminating between their features and functional roles. In order to possibly shed light on these issues, we performed a pilot study in which MVs and EXOs extracted from serum samples of a little cohort of subjects (patients with the first clinical
Few studies have analyzed the effects of total circulating MPs, the physiological or pathophysiological real conditions, on angiogenesis. Recently, we have shown that circulating MPs from peroxisome proliferator-activated receptor-α wild-type mice, but not from knockout mice, induce the formation of capillary-like structures of endothelial cells. This effect, however, was abolished when the NF-κB pathway was inhibited, indicating the involvement of a mechanism dependent on NF-κB activation.50 Thus, peroxisome proliferator-activated receptor-α/NF-κB pathways activated by MPs may play a pivotal stimulatory role for angiogenesis of endothelial cells.. It has been shown that platelet MPs are able to modify steps involved in angiogenesis, such as proliferation, migration, and adhesion of endothelial cells. Kim et al53 were pioneers to demonstrate that platelet MPs increased proliferation, chemotatic migration, and formation of capillary-like tubes of human umbilical vein endothelial cells ...
Extracellular Vesicles (EVs), membrane vesicles released by all cells, are emerging mediators of cell-cell communication. By carrying biomolecules from tissues to biofluids, EVs have attracted attention as non-invasive sources of clinical biomarkers in liquid biopsies. EVs-based liquid biopsies usually require EVs isolation before content analysis, which frequently increases sample volume requirements. We here present a Flow Cytometry (FC) strategy that does not require isolation or concentration of EVs prior to staining. By doing so, it enables population analysis of EVs in samples characterized by challenging small volumes, while reducing overall sample processing time. To illustrate its application, we performed longitudinal non-lethal population analysis of EVs in mouse plasma and in single-animal collections of murine vitreous humor. By quantifying the proportion of vesicular particles in purified and non-purified biological samples, this method also serves as a precious tool to quality control
We have developed an injectable foam suspension containing self-assembling, lipid-based microparticles encapsulating a core of pure oxygen gas for intravenous injection. Prototype suspensions were manufactured to contain between 50 and 90 ml of oxygen gas per deciliter of suspension. Particle size was polydisperse, with a mean particle diameter between 2 and 4 μm. When mixed with human blood ex vivo, oxygen transfer from 70 volume % microparticles was complete within 4 s. When the microparticles were infused by intravenous injection into hypoxemic rabbits, arterial saturations increased within seconds to near-normal levels; this was followed by a decrease in oxygen tensions after stopping the infusions. The particles were also infused into rabbits undergoing 15 min of complete tracheal occlusion. Oxygen microparticles significantly decreased the degree of hypoxemia in these rabbits, and the incidence of cardiac arrest and organ injury was reduced compared to controls. The ability to administer ...
Communication between dying cells and their environment is a critical process that promotes tissue homeostasis during normal cellular turnover, whilst during disease settings, it can contribute to inflammation through the release of intracellular factors. Extracellular vesicles (EVs) are a heterogeneous class of membrane-bound cell-derived structures that can engage in intercellular communication via the trafficking of bioactive molecules between cells and tissues. In addition to the well-described functions of EVs derived from living cells, the ability of dying cells to release EVs capable of mediating functions on target cells or tissues is also of significant interest. In particular, during inflammatory settings such as acute tissue injury, infection and autoimmunity, the EV-mediated transfer of proinflammatory cargo from dying cells is an important process that can elicit profound proinflammatory effects in recipient cells and tissues. Furthermore, the biogenesis of EVs via unique cell-death
Background/Aims: Proteinuria is associated with cardiovascular and chronic kidney disease. Microparticles (MPs) are bioactive vesicles shed from activated cells and also linked to cardiovascular disease. MP-like structures have been identified in the glomerular basement membrane, urinary space and between the glomerular basement membrane and the podocyte. We hypothesised that circulating MPs may provide a link between vascular injury and kidney diseases by inducing podocyte phenotypic alterations, thus propagating glomerular dysfunction and proteinuria. Methods:Human umbilical vein endothelial cells and U937 monocytes were stimulated with TNF-α to produce MPs. These MPs were confirmed by electron microscopy, and added to differentiated podocyte monolayers to determine effects on podocyte albumin endocytosis and the production of soluble mediators. Results:Monocyte and endothelial MPs upregulated podocyte production of pro-inflammatory mediators monocyte chemoattractant protein-1 (p , 0.001) and ...
Extracellular vesicles (EVs) such as exosomes and microvesicles are phospholipid bilayer-enclosed vesicles that are recognized as novel tools for intercellular communications and as biomarkers for several diseases. They contain various DNAs, proteins, mRNAs, and microRNAs (miRNAs) that have potential diagnostic and
Cerebrospinal fluid (CSF) extracellular vesicles (EVs) show promise as a source of neurological disease biomarkers, although their precise origin is poorly understood. Current extraction techniques produce disappointing yield and purity. This study describes the application of ultrafiltration LC (UFLC) to CSF-EVs, compared with ultracentrifugation (UC), and explores CSF-EV origin. EVs are extracted from human CSF by UC and UFLC and characterized using nanoparticle tracking analysis, electron microscopy, and immunoblotting. EV and CSF proteomes are analyzed by LC-MS/MS. UFLC-isolated particles have size, morphology, and marker expression characteristic of EVs. UFLC provides greater EV yield (UFLC 7.90 × 108 ± SD 1.31 × 108 EVs mL-1 CSF, UC 1.06 × 108 ± 0.57 × 108 p | 0.001). UFLC enhances purity, proteomic depth (UFLC 622 ± 49, UC 298 ± 50, p = 0.001), and consistency of quantification (CV 17% vs 23%). EVs contain more intracellular proteins (Odds ratio [OR] 2.63 p | 0.001) and fewer plasma
Cells release extracellular vesicles (EVs) to communicate over long distances, which requires EVs to traverse the extracellular matrix (ECM). However, given that the size of EVs is usually larger than the mesh size of the ECM, it is not clear how they can travel through the dense ECM. Here we show that, in contrast to synthetic nanoparticles, EVs readily transport through nanoporous ECM. Using engineered hydrogels, we demonstrate that the mechanical properties of the matrix regulate anomalous EV transport under confinement. Matrix stress relaxation allows EVs to overcome the confinement, and a higher crosslinking density facilitates a fluctuating transport motion through the polymer mesh, which leads to free diffusion and fast transport. Furthermore, water permeation through aquaporin-1 mediates the EV deformability, which further supports EV transport in hydrogels and a decellularized matrix. Our results provide evidence for the nature of EV transport within confined environments and demonstrate an
Extracellular vesicles (EVs) are nanosized lipid bilayer-bound vesicles that are naturally secreted from most cell types as a communication mechanism to deliver proteins, lipids, and genetic material. Despite the therapeutic potential of EVs, there is limited information on EV uptake kinetics and specificity. Here, we optimized an imaging flow cytometry (IFC)-based platform to quantitatively assess dose, time, and recipient cell specificity effects on human embryonic kidney cell (HEK293T) EV internalization in a high-throughput manner. We found that HEK293T EV uptake is an active process that is dose and time dependent. Further, the selectivity of EV uptake was quantified in vitro, and we found that HEK293T EVs were internalized at higher quantities by cells of the same origin. Lastly, neural stem cells internalized significantly more HEK293T EVs relative to mature neurons, suggesting that stem cells or progenitors, which are more metabolically active than terminally differentiated cells, may have
Introduction: Extracellular vesicles (EVs) are tiny membrane-enclosed vesicles released by cells by membrane budding or exocytosis. The molecular profile of contents and regenerative potential of EVs derived from murine induced pluripotent stem cells (miPSCs) have not been investigated.. Hypothesis: We hypothesized that miPSC-derived EVs carry bioactive substances, including mRNA and miRNA, which would influence the biology and fate of target cells.. Methods: Murine iPSCs were generated using established protocols and EVs were isolated from culture supernatants by sequential centrifugation. Atomic force microscopy (AFM), flow cytometry, and real-time RT-PCR were used to characterize EV contents. Endothelial cells were exposed to miPSC-EVs in culture.. Results: AFM and dynamic light scattering showed that miPSC-EVs are homogenous spherical vesicles smaller than 100 nm (Fig, A). High-sensitivity flow cytometry confirmed the presence of several iPSC- specific markers along with typical exosomal ...
Extracellular vesicles (EVs) produced by fungi are thought to influence pathogenesis and host responses to infection, rendering them potentially important in a medical context, particularly as targets for antivirulence drugs, and candidates for vaccines, for fungal diseases. Among other genera of fungi, EVs have been extracted from Candida, which are responsible for a large proportion of serious and fatal fungal disease cases. However, characterisation of EVs purportedly isolated from culture supernatants of Candida albicans may have resulted in erroneous conclusions being drawn regarding their physical attributes, functions and molecular cargo, due to a lack of purification of these structures from contaminants such as protein aggregates. This work aimed to investigate whether the ultracentrifugation-based extraction methods in previous studies were likely adequate enough to isolate C. albicans EVs from other entities, or whether additional purification procedures, namely size-exclusion ...
Extracellular Vesicles (EVs) are lipid vesicles secreted by cells that allow intercellular communication. They are decorated with surface proteins, which are membrane proteins that can be targeted by biochemical techniques to isolate EVs from background particles. EVs have recently attracted attention for their pot
Exosomes are nanometer-sized extracellular vesicles that are believed to function as intercellular communicators. and irradiated recipient cells. We found an enhanced uptake of exosomes isolated Pyronaridine Tetraphosphate from both irradiated and non-irradiated cells by irradiated recipient cells compared to nonirradiated recipient cells. Functional analyses by exosome transfer indicated that all exosomes (from non-irradiated and irradiated donor cells) increase the proliferation of non-irradiated recipient cells and the survival of irradiated recipient cells. The survival-promoting effects are more pronounced when exosomes isolated from irradiated compared to non-irradiated donor cells are transferred. A possible mechanism for the increased survival after irradiation could be the increase in DNA double-strand break repair monitored at 6 8 and 10 h after the transfer of exosomes isolated from irradiated cells. This is abrogated by the destabilization of the exosomes. Our results demonstrate ...
During human pregnancy, placental trophectoderm cells release extracellular vesicles (EVs) into maternal circulation. Trophoblasts also give rise to cell-free DNA (cfDNA) in maternal blood, and has been used for noninvasive prenatal screening for chromosomal aneuploidy. We intended to prove the existence of DNA in the EVs (evDNA) of maternal blood, and compared evDNA with plasma cfDNA in terms of genome distribution, fragment length, and the possibility of detecting genetic diseases. Maternal blood from 20 euploid pregnancies, 9 T21 pregnancies, 3 T18 pregnancies, 1 T13 pregnancy, and 2 pregnancies with FGFR3 mutations were obtained. EVs were separated from maternal plasma, and confirmed by transmission electronic microscopy (TEM), western blotting, and flow cytometry (FACS). evDNA was extracted and its fetal origin was confirmed by quantitative PCR (qPCR). Pair-end (PE) whole genome sequencing was performed to characterize evDNA, and the results were compared with that of cfDNA. The fetal risk of
An efficient method of producing purified extracellular vesicles (EVs), in conjunction with a method that blocks liver macrophages from clearing EVs from the body, has produced promising results for the use of EVs in cancer therapy.
Extracellular vesicles (EVs), including exosomes and microvesicles, have emerged as promising drug delivery vehicles for small RNAs (siRNA and miRNA) due to their natural role in intercellular RNA...
Extracellular vesicles (EVs) are released by mammalian cells and are thought to be important mediators of intercellular communication. There are many methods for isolating EVs from cell culture...
Exosomes and microvesicles, collectively referred to as small extracellular vesicles (sEV) are vesicles with an average size of about 100-150 nm. Currently, the role of sEV in various aspects of signaling in the body is being actively investigated; in addition, sEV can often serve as markers of various pathologies. The active study of the sEV composition is continuing. In this study we have demonstrated that in sEV it is possible to determine cholesterol and triglycerides concentration by using commercial kits designed for serum. The technique was tested on sEV from the blood of patients diagnosed with depression and on healthy volunteers. No differences were found in the concentration of cholesterol and triglycerides in mEV from the blood serum of depressed patients and the control group. The concentration of cholesterol and triglycerides in the samples is several times higher than the sensitivity threshold of the methods set by the manufacturer of the kits.. ...
Microparticles are derived from many other cell types. Microvesicles and exosomes are formed and released by two slightly ... Microparticles derived from monocytes aggravate atherosclerosis by modulating inflammatory cells. Additionally, microvesicles ... For example, those released from antigen-presenting cells (APCs), such as B cells and dendritic cells, are enriched in proteins ... Once released from their cell of origin, microvesicles interact specifically with cells they recognize by binding to cell-type ...
"Macrophages discriminate glycosylation patterns of apoptotic cell-derived microparticles". The Journal of Biological Chemistry ... Although most cells are capable of phagocytosis, some cell types perform it as part of their main function. These are called ' ... One of the features of an apoptotic cell is the presentation of a variety of intracellular molecules on the cell surface, such ... Phagocytosis (from Ancient Greek φαγεῖν (phagein) 'to eat', and κύτος, (kytos) 'cell') is the process by which a cell uses its ...
It is derived from the bacteria that causes acne and targets myeloid cells through TLR9 and NOD2. In one of its first rounds of ... MIS416 is a microparticle derived from the cytoskeleton of P. acnes, a species of bacteria present on the skin of most adults ... Bacteria-derived microparticles have several advantages over traditional adjuvants related both to their size and biological ... a non-toxic microparticle adjuvant derived from Propionibacterium acnes comprising immunostimulatory muramyl dipeptide and ...
Kumacheva has made strong contributions in the area of self-assembly of nanoparticles and microparticles. Her research ... affect necrotic heart tissue as well as deter the metastasis of cancer cells. ... interests include nature-derived nanopartocles, e.g., cellulose nanocrystals and nanofibers ). Eugenia Kumacheva's awards and ... Kumacheva's work is the creation of hydrogels and various other chemical environments to either support the life of a stem cell ...
MIS416, a non-toxic microparticle adjuvant derived from Propionibacterium acnes comprising immunostimulatory muramyl dipeptide ... and Th17 cells in EAE, and [increased] the serum levels of IFN-γ and IFN-γ-associated proteins in 19 patients with SPMS." " ... "a myeloid-directed microparticle immune response modifier (derived from Cutibacterium acnes), which was originally developed as ... a non-toxic microparticle adjuvant derived from Propionibacterium acnes comprising immunostimulatory muramyl dipeptide and ...
"Detection of fetal DNA and RNA in placenta-derived syncytiotrophoblast microparticles generated in vitro". Clinical Chemistry. ... Cell-free fetal DNA (cffDNA) is fetal DNA that circulates freely in the maternal blood. Maternal blood is sampled by ... Approximately 11 to 13.4 percent of the cell-free DNA in maternal blood is of fetal origin. The amount varies widely from one ... Smaller fragments can represent up to seventy percent of the total cell free DNA in the maternal blood sample. In real-time PCR ...
... the HIV co-receptors CCR5 and CXCR4 could be transferred from an HIV-susceptible cell to a refractory cell by "microparticles ... A clinical trial of dendritic cell-derived EVs was performed in France just before the turn of the century.[citation needed] ... Since apoptotic cells tend to display phosphatidylserine (PS) in the outer bilayer of the cell membrane, apoptotic bodies tend ... They carry a cargo of proteins, nucleic acids, lipids, metabolites, and even organelles from the parent cell. Most cells that ...
Caveolae Cellular dewetting Endothelial activation Endothelial microparticle Endothelial progenitor cell Endothelium-derived ... Endothelial cells in direct contact with blood are called vascular endothelial cells whereas those in direct contact with lymph ... Endothelial cells actively signal to white blood cells of the immune system during inflammation Formation of new blood vessels ... Vascular endothelial cells line the entire circulatory system, from the heart to the smallest capillaries. These cells have ...
Martínez, MC; Tesse, A; Zobairi, F; Andriantsitohaina, R. Shed membrane microparticles from circulating and vascular cells in ... monocyte-derived and tissue factor-carrying circulating microparticles are related to acute myocardial infarction severity.. ... 胞漿顆粒(英语:Granule (cell biology)). 黑色素體 · 微粒體 · 過氧化物酶體 · 乙醛酸循環體 · 韋伯潘力氏小體(英语:Weibel-Palade body) ... A whole-cell electron tomography model of vacuole biogenesis in Arabidopsis root cells.. Nature plants
"Calculation of Single Cell Assimilation Rates From SIP-NanoSIMS-Derived Isotope Ratios: A Comprehensive Approach". Frontiers in ... NanoSIMS can also be used to examine the elemental and isotopic composition of microparticles preserved in the rock record. The ... The mouse gut microbiome was investigated to determine which microbes fed on host-derived compounds. For this, mice were given ... Peteranderl, R.; Lechene, C. (2004-04-01). "Measure of carbon and nitrogen stable isotope ratios in cultured cells". Journal of ...
In many plants, the laticiferous system is formed from rows of cells laid down in the meristem of the stem or root. The cell ... Since the 17th century, latex has been used as a term for the fluid substance in plants, deriving from the Latin word for " ... Latex is a stable dispersion (emulsion) of polymer microparticles in water. Latexes are found in nature, but synthetic latexes ... In the mature plant, the entire laticiferous system is descended from a single cell or group of cells present in the embryo. ...
Agarose is a polysaccharide derived from seaweed used for nanoencapsulation of cells and the cell/agarose suspension can be ... Alginate microparticles loaded with myoblast cells and functionalized with RGD allowed control over the growth and ... autologous cells), from another donor (allogeneic cells) or from other species (xenogeneic cells). The use of autologous cells ... The cell type chosen for this technique depends on the desired application of the cell microcapsules. The cells put into the ...
Moving into the cell membrane is the transmembrane domain, which is followed by the spacer domain located outside of the cell, ... "Macrophage scavenger receptors and host-derived ligands". Methods. 43 (3): 207-17. doi:10.1016/j.ymeth.2007.06.004. PMID ... "Therapeutic inflammatory monocyte modulation using immune-modifying microparticles". Science Translational Medicine. 6 (219): ... When ligand binds to MARCO on dendritic cells, the cytoskeleton of the cell is altered, allowing for the formation of the long ...
... dioxide Cotton Charcoal Smoke particulates Air pollutants Cosmetics-derived microparticles Drug nanoparticles Implant-derived ... Cells undergo cell death via three main mechanisms: nectoptosis via RIPK1, FADD, RIPK3, and MLKL, ferroptosis via GPX4 ... Necroinflammation is an autoamplifying process where crystals are toxic to cells (cytotoxicity) and cause cell death (necrosis ... form foam cells and giant cells, and try to do extracellular digestion in a process called frustrated phagocytosis. Crystals do ...
When used as scaffolds, hydrogels may contain human cells to repair tissue. They mimic 3D microenvironment of cells. Hydrogel- ... Other additives, such as nanoparticles and microparticles, have been shown to significantly modify the stiffness and gelation ... "The use of chitosan based hydrogel for enhancing the therapeutic benefits of adipose-derived MSCs for acute kidney injury". ... coated wells have been used for cell culture. Investigating biomechanical functions in cells when combined with Holotomography ...
"3.3 A cryo-EM structure of a nonenveloped virus reveals a priming mechanism for cell entry". Cell. 141 (3): 472-82. doi:10.1016 ... and micro-particles gave rise to a detailed publication, Ref. Degueldre et al (2006).[] Since 2010 the interest for SP ICP-MS ... "Dynamics in cryo EM reconstructions visualized with maximum-likelihood derived variance maps". Journal of Structural Biology. ... Å resolution CryoTEM structure of β-galactosidase in complex with a cell-permeant inhibitor". Science. 348 (6239): 1147-1151. ...
"Encapsulating bacteria in agarose microparticles using microfluidics for high-throughput cell analysis and isolation". ACS ... More recent advancements in microfluidic particles allowed the synthesis of nanometer sized particles from biologically derived ... Hydrocarbon oil is typically not used in cell microfluidic research because it is not compatible with cells and damages cell ... Droplets containing cells can be stored off-chip in PTFE tubing for up to several days while maintaining cell viability and ...
... but some compounds from the cell wall are unique and are used to trace bacterial derived DOC (e.g., peptidoglycan). These ... Self-organization of dissolved organic matter tomicelle-like microparticles in river water. Nature 422, 150-154. doi: 10.1038/ ... Bacteria are often viewed as the main consumers of DOC, but they can also produce DOC during cell division and viral lysis. The ... The lytic cycle causes disruption of the cell(s) and release of DOC. Marine macrophytes (i.e., macroalgae and seagrass) are ...
A theoretical expression for this spectrum is derived in the following, which can then be fitted to the experimental curve in ... Other applications are the rheology of soft matter, and studies of force-regulated processes in living cells. Forces are ... A magnetic tweezers apparatus consists of magnetic micro-particles, which can be manipulated with the help of an external ... In experiments, the molecule of interest is attached to a magnetic microparticle. The magnetic tweezer is equipped with magnets ...
These cells, together with other immune cells such as macrophages, lymphocytes, neutrophils, mast cells, dendritic cells and ... The word "synovium" is derived from the word "synovia" (or synovial fluid), which is a clear, viscous fluid produced by the ... 2010). "Platelets Amplify Inflammation in Arthritis via Collagen-Dependent Microparticle Production". Science. 327 (5965): 580- ... These hallmark features of FLS in RA are divided into 7 cell-intrinsic hallmarks and 4 cell-extrinsic hallmarks. The cell- ...
Cancer cells may facilitate export of PAI-2 via microparticles.[19] PAI-2 provides protection for cancer cells against plasmin- ... "Cloning and expression of a cDNA coding for a human monocyte-derived plasminogen activator inhibitor". Proceedings of the ... Among immune cells, macrophages are the main producers of PAI-2, as both B-cells and T-cells do not produce significant amounts ... "Cell. 156 (5): 1002-16. doi:10.1016/j.cell.2014.01.040. PMC 3988473. PMID 24581498.. ...
... has shown to induce apoptosis and promote antitumor actions on various cancer cell lines in vitro. Hinokinin has been ... Hinokinin is a dibenzylbutyrolactone lignan, derived from various species of plants. It is a potential antichagonistic agent ... microparticles for Chagas disease". Parasitology Research. 106 (3): 703-708. doi:10.1007/s00436-010-1725-1. ISSN 0932-0113. ...
A thick SEI layer results in a higher cell resistance, which decreases cell efficiency. The SEI layer on silicon is composed of ... These microparticles reached an energy density of 3,300 mAh/g. In 2015, Tesla founder Elon Musk claimed that silicon in Model S ... Polymer derived SiOC integrated with graphene aerogel as highly stable Li-ion battery anodes ACS Appl. Mater. Interfaces 2020, ... Lithium-silicon batteries also include cell configurations where Si is in compounds that may at low voltage store lithium by a ...
... antimetastatic activity of dendritic cells based on vaccines when mechanically heterogenized microparticles of tumor cells are ... One such model, the patient derived tumour xenograft, has shown excellent utility in preserving tumour heterogeneity whilst ... The cancer stem cell model asserts that within a population of tumour cells, there is only a small subset of cells that are ... There are two models used to explain the heterogeneity of tumour cells. These are the cancer stem cell model and the clonal ...
Cross-priming occurs when a bone marrow-derived cell presents peptides from proteins synthesised in another cell in the context ... that has been absorbed onto gold or tungsten microparticles into the target cells, using compressed helium as an accelerant. ... Studies using chimeric mice have shown that antigen is presented by bone-marrow derived cells, which include dendritic cells, ... The type of T-cell help raised is determined by the differentiated state of antigen presenting cells. Dendritic cells can ...
Contaminants inside the cells can defeat these safety devices. Also, these features can not be applied to all kinds of cells, e ... Polymer-Derived SiOC Integrated with a Graphene Aerogel As a Highly Stable Li-Ion Battery Anode ACS Appl. Mater. Interfaces ... Qi, Zhaoxiang; Koenig, Gary M. (16 August 2016). "High-Performance LiCoO2Sub-Micrometer Materials from Scalable Microparticle ... The 21700 cell has 50% more energy than the 18650 cell, and the bigger size reduces heat transfer to its surroundings. The ...
Using light microscopy, he observed macrophage cells laden with micro-particles in lymph nodes of the affected extremity. After ... the result of a genetically determined abnormal inflammatory reaction to mineral particles in irritant red clay soils derived ...
In general, it is used to incorporate food ingredients, enzymes, cells or other materials on a micro metric scale. ... Interfacial cross-linking is derived from interfacial polycondensation, and was developed to avoid the use of toxic diamines, ... IUPAC definition Microcapsule: Hollow microparticle composed of a solid shell surrounding a core-forming space available to ... "The Gut Microbiota and Human Health with an Emphasis on the Use of Microencapsulated Bacterial Cells", Journal of Biomedicine ...
One of the most common sources of β(1,3)D-glucan for supplement use is derived from the cell wall of baker's yeast ( ... "Role of the glycocalyx in regulating access of microparticles to apical plasma membranes of intestinal epithelial cells: ... β-Glucans (beta-glucans) comprise a group of β-D-glucose polysaccharides naturally occurring in the cell walls of cereals, ... β-glucans form a natural component of the cell walls of bacteria, fungi, yeast, and cereals such as oat and barley. Each type ...
positive regulation of blood microparticle formation. • negative regulation of endothelial cell proliferation. • positive ... As most information regarding TNF signaling is derived from TNFR1, the role of TNFR2 is likely underestimated. ... but it is produced also by a broad variety of cell types including lymphoid cells, mast cells, endothelial cells, cardiac ... regulation of heterotypic cell-cell adhesion. • negative regulation of mitotic cell cycle. • endothelial cell apoptotic process ...
The word galactose was coined by Charles Weissman[5] in the mid 19th century and is derived from Greek galaktos (milk) and the ... Galactose is a component of the antigens present on blood cells that determine blood type within the ABO blood group system. In ... Consequently, various food products made with dairy-derived ingredients can contain lactose.[9] Galactose metabolism, which ...
positive regulation of macrophage derived foam cell differentiation. • positive regulation of cholesterol storage. • plasma ... positive regulation of blood microparticle formation. • positive regulation of reactive oxygen species metabolic process. ... cell adhesion. • cell surface receptor signaling pathway. • nitric oxide mediated signal transduction. • blood coagulation. • ... cell surface. • membrane. • endocytic vesicle membrane. • platelet alpha granule membrane. • membrane raft. ...
Microparticles of carbon are produced in diesel engine exhaust fumes, and may accumulate in the lungs.[124] In these examples, ... see diamond anvil cell), high-performance bearings, and limited use in specialized windows.[120][121] With the continuing ... the most important energy-transfer molecule in all living cells. ...
The crude feed, a mixture of soluble proteins, contaminants, cells, and cell debris, is then passed upward through the expanded ... Chromatography, pronounced /ˌkroʊməˈtɒɡrəfi/, is derived from Greek χρῶμα chroma, which means "color", and γράφειν graphein, ... Jellema LJ, Markesteijn AP, Westerweel J, Verpoorte E (May 2010). "Tunable hydrodynamic chromatography of microparticles ... Programmed cell death Apoptosis. Pyknosis. Karyorrhexis. Karyolysis. Accumulations. pigment Hemosiderin. Lipochrome/Lipofuscin ...
... normal physiologic coagulation requires the presence of two cell types for formation of coagulation complexes: cells that ... Many analysers are capable of measuring a "derived fibrinogen" level from the graph of the Prothrombin time clot. ... as well as procoagulant microparticles or microvesicles shed from them. Calcium is also required at other points in the ... Mediates cell adhesion. Glomerulopathy with fibronectin deposits antithrombin III. Inhibits IIa, Xa, and other proteases. ...
Cells of interest in such cases are endothelial cells of blood vessels, and leukocytes: *The inflamed blood vessels ... Targeting ligands can be immunogenic, since current targeting ligands used in preclinical experiments are derived from animal ... such as inflamed cells or cancer cells. Current microbubbles in development are composed of a lipid monolayer shell with a ... Cancer: cancer cells also express a specific set of receptors, mainly receptors that encourage angiogenesis, or the growth of ...
Thus laden with high levels of POPs, the microparticles are often ingested by organisms in the biosphere[citation needed]. ... Extensive studies in Europe indicate that the chlorine found in emitted dioxins is not derived from HCl in the flue gases. ... see closed-cell PVC foamboard). Sheets are cut using saws and rotary cutting equipment. Plasticized PVC is also used to produce ... Weathering degradation of plastics results in their surface embrittlement and microcracking, yielding microparticles that ...
... s have no cell nucleus: they are fragments of cytoplasm that are derived from the megakaryocytes[2] of the bone marrow ... "Platelets amplify inflammation in arthritis via collagen-dependent microparticle production". Science. 327 (5965): 580-3. ... Berridge, Michael J. (1 October 2014). "Module 11: Cell Stress, Inflammatory Responses and Cell Death". Cell Signalling Biology ... "Programmed anuclear cell death delimits platelet life span". Cell. 128 (6): 1173-86. doi:10.1016/j.cell.2007.01.037. PMID ...
Secondary cells and batteries containing alkaline or other non-acid electrolytes - Secondary lithium cells and batteries for ... Qi, Zhaoxiang; Koenig, Gary M. (16 August 2016). "High-Performance LiCoO2Sub-Micrometer Materials from Scalable Microparticle ... "Layered Lithium-Manganese Oxide Electrodes Derived from Rock-Salt LixMnyOz (x+y=z) Precursors" 194th Meeting of the ... Battery versus cellEdit. A cell is a basic electrochemical unit that contains the electrodes, separator, and electrolyte.[19][ ...
positive regulation of macrophage derived foam cell differentiation. • regulation of vasoconstriction. • positive regulation of ... blood microparticle. • extracellular space. • cytosol. • extracellular region. Biological process. • uterine smooth muscle ... regulation of cell proliferation. • regulation of cell growth. • positive regulation of cell proliferation. • fibroblast ... cell-cell signaling. • renin-angiotensin regulation of aldosterone production. • aging. • vasodilation. • positive regulation ...
Katie Fehrenbacher New Rechargeable Cell Has 7 Times Higher Energy Density Than Li-ion Cells, Nikkei Technology, 23 July 2014, ... researchers at Nanyang Technological University used a materials derived from a titanium dioxide gel derived from naturally ... "High-Performance LiCoO2Sub-Micrometer Materials from Scalable Microparticle Template Processing". ChemistrySelect. 1 (13): 3992 ... researchers from CMU found that prismatic cells are more likely to benefit from production scaling than cylindrical cells. ...
... that cells in the G2 Phase are more resistant to radiation than cells in the M Phase (Radiosensitivity and Cell cycle). The ... "The interaction of natural background gamma radiation with depleted uranium micro-particles in the human body". Journal of ... Loads and Fate of Fertilizer Derived Uranium. Backhuys Publishers, Leiden, The Netherlands, ISBN 978-90-5782-193-6. Koppe JG, ... According to SET, primary exposure to a beta particle alters a cell to the G2 Phase, which Busby claims would render the cell ...
"Controlled Inhibition of the Mesenchymal Stromal Cell Pro-inflammatory Secretome via Microparticle Engineering". Stem Cell ... working on the development of regenerative therapeutic methods by collecting patient-derived induced pluripotent stem cells ( ... She represents India in the International Stem Cell Initiative (ISCI) and the International Stem Cell Banking Initiative (ISCBI ... Inamdar's group works on the basic biology of stem cells . The group led by her has developed normal stem cell lines from ...
The device for which SPR is derived includes a sensor chip, a flow cell, a light source, a prism, and a fixed angle position ... "SIMAG Basic : Magnetic nano and micro particles". "SIMAG Affinity : Magnetic nano and micro particles by chemicell". "Home - ... cells transfected with cloned receptors, and cells that are either in culture or isolated prior to analysis. Saturation binding ... In this type of assay the binding of a ligand to cells is followed over time. The obtained signal is proportional to the number ...
One of the most common sources of β(1,3)D-glucan for supplement use is derived from the cell wall of baker's yeast ( ... "Role of the glycocalyx in regulating access of microparticles to apical plasma membranes of intestinal epithelial cells: ... β-glucans form a natural component of the cell walls of bacteria, fungi, yeast, and cereals such as oat and barley. Each type ... M cells within the Peyer's patches physically transport the insoluble whole glucan particles into the gut-associated lymphoid ...
... normal physiologic coagulation requires the presence of two cell types for formation of coagulation complexes: cells that ... Many analysers are capable of measuring a "derived fibrinogen" level from the graph of the Prothrombin time clot. If a ... as well as procoagulant microparticles or microvesicles shed from them. Calcium is also required at other points in the ... First is the initiation phase, which occurs in tissue-factor-expressing cells. This is followed by the propagation phase, which ...
2017). "Graft-derived cell-free DNA, a noninvasive early rejection and graft damage marker in liver transplantation: A ... Diehl F, Li M, Kinzler, KW, Vogelstein B, Dressman D (2006). "BEAMing: single-molecule PCR on microparticles in water-in-oil ... 2017). "Single Cell-Based Vector Tracing in Patients with ADA-SCID Treated with Stem Cell Gene Therapy". Molecular Therapy - ... 2016). "Digital Quantification of Proteins and mRNA in Single Mammalian Cells". Molecular Cell. 61 (6): 914-24. doi:10.1016/j. ...
Atherosclerosis refers to a progressive narrowing of the arteries due to atheroma, derived from the Greek word for gruel or ... ISBN 978-0-19-157556-3. Wah TM (August 2017). "Image-guided ablation of renal cell carcinoma". Clinical Radiology. Elsevier BV ... Drug eluting bead transarterial chemoembolization (DEB-TACE): delivery of microparticles that are themselves loaded with the ... Cryoablation Cryoablation is a treatment option that destroys cancer cells by applying extremely cold temperatures at the ...
PDMP, monocyte-derived microparticles (MDMP), and endothelial cell-derived microparticles (EDMP) were detected by flow ... Nomura, S., Ishii, K., Kanazawa, S. et al. Significance of elevation in cell-derived microparticles after allogeneic stem cell ... Significance of elevation in cell-derived microparticles after allogeneic stem cell transplantation: transient elevation of ... Significance of elevation in cell-derived microparticles after allogeneic stem cell transplantation: transient elevation of ...
Cell-derived microparticles (MPs) have been described as vital contributors to the inflammatory process. However, its role in ... Keywords : chronic periodontitis; diabetes; gingival crevicular fluid; cell-derived microparticles. · abstract in Portuguese · ... Cell Derived Microparticles in Gingival Crevicular Fluid from Periodontitis Patients with Type 2 Diabetes. Braz. Dent. J. [ ... Therefore, we aimed to detect the presence neutrophil (CD66b+) and platelet (CD41b+) derived microparticles in gingival ...
A Useful Biomarker for Predicting One-Year Mortality in Patients with Advanced Non-Small Cell Lung Cancer. Chin-Chou Wang,1,2,3 ... platelet-derived apoptotic MPs (PDAp-MPs), (3) endothelial-derived activated MPs (EDAc-MPs), and (4) endothelial-derived ... This study tested the hypothesis that circulating microparticles (MPs) are useful biomarkers for predicting one-year mortality ... Levels of four MPs in circulation, (1) platelet-derived activated MPs (PDAc-MPs), (2) ...
We propose that these microparticles contribute to the local hypercoagulation and fibrin deposition in inflamed joints of ... Synovial fluid contains high numbers of microparticles derived from leukocytes that are strongly coagulant via the factor VII- ... Cell-derived microparticles in synovial fluid from inflamed arthritic joints support coagulation exclusively via a factor VII- ... Conclusion: Synovial fluid contains high numbers of microparticles derived from leukocytes that are strongly coagulant via the ...
... recently reported to associate with elevated microvesicle/microparticle (MP) counts, have also long been known to be ... Detection and Isolation of Cell-Derived Microparticles Are Compromised by Protein Complexes Resulting From Shared Biophysical ... These data may necessitate reevaluation of certain published data on patient-derived MPs and contribute to correct the clinical ... Numerous diseases, recently reported to associate with elevated microvesicle/microparticle (MP) counts, have also long been ...
The Impact of Sickle Cell Disease Induced Oxidative Stress on MP Proteome ... Analysis of RBC Derived Microparticles from Sickle Cell Mice using Mass Spectrometry: ... Analysis of RBC Derived Microparticles from Sickle Cell Mice using Mass Spectrometry: The Impact of Sickle Cell Disease Induced ... This seminar, will also focus on proteomic differences between Microparticles derived from wildtype and SCD transgenic mouse ...
T cell gate. (C) IFN-γ+ cells and IL-2+ cells (n = 9) within CD45+CD3+CD4+ cells. (D) IFN-γ+ cells and IL-2+ cells (n = 9) ... Reversing drug resistance of soft tumor-repopulating cells by tumor cell-derived chemotherapeutic microparticles. Cell Res. 26 ... B) Lymphocyte infiltrates including CD45+CD3+ T cells, CD45+CD3+CD4+ T cells, CD45+CD3+CD8+ T cells, CD4/CD8 T cell ratio, and ... F) Lymphocyte infiltrates including CD45+CD3+ T cells, CD45+CD3+CD4+ T cells, and CD45+CD3+CD8+ T cells. (G) CD4/CD8 T cell ...
Derived Cells Induced by High‐Fat Diet. ...
Measurement of circulating cell-derived microparticles by flow cytometry: Sources of variability within the assay ... INTRODUCTION: Circulating cell-derived microparticles (MPs) have been implicated in several disease processes and elevated ... INTRODUCTION: Circulating cell-derived microparticles (MPs) have been implicated in several disease processes and elevated ... Measurement of circulating cell-derived microparticles by flow cytometry: Sources of variability within the assay. Thrombosis ...
Red cell-derived microparticles (RMP) as haemostatic agent. Thromb Haemost. 2013;110(4):751-760.. View this article via: PubMed ... Circulating cell membrane microparticles transfer heme to endothelial cells and trigger vasoocclusions in sickle cell disease. ... Nitric oxide scavenging by red blood cell microparticles and cell-free hemoglobin as a mechanism for the red cell storage ... Townes-SS RBC-derived microparticles promote toxicity in human endothelial cells. (A) Oxidative toxicity in human umbilical ...
The absolute counting of red cell-derived microparticles with red cell bead by flow rate based assay ... The absolute counting of red cell-derived microparticles with red cell bead by flow rate based assay ... The absolute counting of red cell-derived microparticles with red cell bead by flow rate based assay ...
Keywords: Microparticles, Acute Coronary Syndromes, Endothelium-derived Microparticles, Platelet-derived Microparticles, Acute ... Keywords:Microparticles, Acute Coronary Syndromes, Endothelium-derived Microparticles, Platelet-derived Microparticles, Acute ... Cell-Derived Microparticles and Acute Coronary Syndromes: Is there a Predictive Role for Microparticles?. Author(s): Effimia ... Title:Cell-Derived Microparticles and Acute Coronary Syndromes: Is there a Predictive Role for Microparticles? ...
Comparison of exosomes secreted by induced pluripotent stem cell-derived mesenchymal stem cells and synovial membrane-derived ... B) Determination of the percentage of CD4+IFNγ+ Th1 cells, CD4+IL17+ Th17 cells, CD4+Foxp3+ Treg cells, CD4+IL10+ Tr1 cells, ... cytotoxic T cells, CD4+IFN-γ+ Th1 cells, CD4+IL-10+ Tr1 cells and CD4+CD25+Foxp3+ Treg cells in splenocytes (n=4) when ... Human mesenchymal stem cell-derived microvesicles modulate T cell response to islet antigen glutamic acid decarboxylase in ...
... derived from kidney-derived mesenchymal stem cells (KMSCs) have recently been reported to ameliorate rarefaction of peritubular ... In vivo administration of KMSC-derived MPs significantly inhibited EndoMT of PTC endothelial cells and improved PTC rarefaction ... Furthermore, administration of KMSC-derived MPs inhibited inflammatory cell infiltration as well as tubulointerstitial fibrosis ... KMSC-derived MPs significantly ameliorated EndoMT and improved in vitro proliferation of TGF-β1 treated HUVEC. ...
... their role in SCA eryptosis and their impact on the genesis of RBC-derived microparticles (RBC-MPs) remains poorly described. ... however their role in SCA eryptosis and their impact on the genesis of RBC-derived microparticles (RBC-MPs) remains poorly ... the effects of SCA/AA-RBC-MPs on human aortic endothelial cell (HAEC) inflammatory phenotype and TLR4 pathway. Eryptosis, RBC- ... the effects of SCA/AA-RBC-MPs on human aortic endothelial cell (HAEC) inflammatory phenotype and TLR4 pathway. Eryptosis, RBC- ...
... and for adhesive cells including adipose-derived stromal cells (ADSCs) and bone marrow-derived mesenchymal stem cells (BMSCs). ... Furthermore, LMWH/P N/MPs can also bind to adhesive cell surfaces, inducing cells and LMWH/P N/MPs-aggregate formation. Those ... cultures of cells including ADSCs and BMSCs using plasma-medium gel with LMWH/P N/MPs exhibited efficient cell proliferation. ... LMWH/P N/MPs can also bind to tissue culture plates and adsorb exogenous GFs or GFs from those cells. The LMWH/P N/MPs-coated ...
Cell-derived Microparticles. Extracellular membrane vesicles generated by the shedding of CELL MEMBRANES blebs. Microparticles ... Membrane microparticles are submicron fragments of membrane vesicles shed from various cell types. Circulating endothelial ... Microparticles are used in a variety of different fields, such as drug delivery. Recently, non-spherical microparticle ... and other cell types circulate in the peripheral blood and through the MICROVASCULATURE where larger cells cannot, functioning ...
CONCLUSION(S) Cell-derived microparticles were raised in normal pregnancy. Recurrent miscarriage seems to be related to ... endothelial and platelet cell activation and/or consumption. An increase in almost all cMP types was observed in PE. A ... OBJECTIVE To assess current studies on the relationship between cell-derived microparticles (cMP) and recurrent miscarriages ( ... Circulating cell-derived microparticles in severe preeclampsia and in fetal growth restriction.. Jaume Alijotas-Reig, Carles ...
Circulating cell-derived microparticles in malaria patients Nantakomon D., Udomsangpetch R., Pattanapunyasat K., Looareesuwan S ...
Cell-Derived Microparticles Promote Coagulation after Moderate Exercise. SOSSDORF, MAIK; OTTO, GORDON P.; CLAUS, RALF A.; More ...
... monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T ... are excellent vehicles for releasing molecules inside the cell. The aim of this work was to use MSMPs to deliver viral specific ... MHC class I restricted epitopes into human antigen presenting cells ( ... The microparticles are internalized into dendritic cells (DC). (a) Photographs show control cells (MDDCs) or with internalized ...
... circulating levels of endothelial cell-derived microparticles and bone marrow-derived endothelial progenitor cells, and indices ... endothelial cell injury, as represented by elevated levels of circulating endothelial cell-derived microparticles (EMPs), and ... Endothelial cell-derived microparticles. EC injury is assessed by measuring circulating EMPs, which are markers of activated or ... Cellular biomarkers of endothelial health: microparticles, endothelial progenitor cells, and circulating endothelial cells. J ...
Improving the dissolution of fenofibrate with yeast cell-derived hollow core/shell carbon microparticles. In: RSC Advances. ... Improving the dissolution of fenofibrate with yeast cell-derived hollow core/shell carbon microparticles. RSC Advances. 2016 ... Improving the dissolution of fenofibrate with yeast cell-derived hollow core/shell carbon microparticles. / Huang, Xiaoxi; ... title = "Improving the dissolution of fenofibrate with yeast cell-derived hollow core/shell carbon microparticles", ...
Circulating Endothelium-Derived Microparticles (EMPs). Preparation of platelet-free plasma. Endothelium-derived microparticles ... endothelial microparticle. EPC. endothelial progenitor cell. FMD. flow-mediated dilation. L-NMMA. LG-monomethyl-arginine. NO. ... 2003) Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis. Thromb ... 2003) Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis. Thromb ...
Neutrophil microparticles-induced injuries and morphological alterations in human umbilical vein endothelial cells (HUVECs) ... In this study we investigated the potential role of myeloperoxidase in the endothelial cell injury caused by neutrophil-derived ... Exposure of HUVEC to neutrophil microparticles induced a loss of cell membrane integrity and morphological changes. The ... in neutrophil microparticles and that the microparticle-associated myeloperoxidase cause injury to endothelial cells. Hence, ...
Levels of circulating EPC-microparticles (MPs) and endothelium-derived MPs (EMPs) in db/db mice are changed in basal and after ... A and B: the P1 gate for CD45+ cells. C-F: EPCs are defined as CD34+/VEGFR+ (top right) cells gated on P1 gate (C: db/+ sham; D ... Circulating endothelial progenitor cells and cellular membrane microparticles in db/db diabetic mouse: possible implications in ... Circulating endothelial progenitor cells and cellular membrane microparticles in db/db diabetic mouse: possible implications in ...
Thus, microparticles can transfer chemokine receptors and arachidonic acid between cells, activate complement, promote ... A role in inflammatory arthritis is suggested by the finding that leukocyte-derived microparticles induce the production of ... Under certain conditions, however, microparticles may also exert anti-inflammatory properties by inducing immune cell apoptosis ... Microparticles may play an important role in the pathogenesis of rheumatologic diseases as evidenced by their elevation in ...
Cell-cell communication has proven to be even more complex than previously thought since the discovery that extracellular ... Cell-derived microparticles. *Exosomes. *Microrna. Get free article suggestions today. Mendeley saves you time finding and ... Cell-cell communication has proven to be even more complex than previously thought since the discovery that extracellular ... More than simply cell dust, extracellular vesicles are capable of transferring biological information to neighboring cells and ...
Cell-Derived Microparticles in Patients with Type 2 Diabetes Mellitus: a Systematic Review and Meta-Analysis ... Type 2 diabetes mellitus Microparticles Biomarker Platelet-derived microparticle Endothelium-derived microparticle ... Supplementary Material for: Cell-Derived Microparticles in Patients with Type 2 Diabetes Mellitus: a Systematic Review and Meta ... i,Background/Aims: ,/i,The aim of this study was to assess the association between circulating cell-derived microparticles (MPs ...
Circulating cell-derived microparticles (MPs) are sub-micronic phospholipid vesicles derived from cells of different types in ... Cell-derived microparticles (MPs) and their role in unexplained recurrent pregnancy loss * Rucha Kiran Patil National Institute ... There are many studies which show the association of specific cell-derived MPs to different pathological states. MPs act as a ...
  • PDMP, monocyte-derived microparticles (MDMP), and endothelial cell-derived microparticles (EDMP) were detected by flow cytometry using a modified version of the previously reported method. (nature.com)
  • Levels of four MPs in circulation, (1) platelet-derived activated MPs (PDAc-MPs), (2) platelet-derived apoptotic MPs (PDAp-MPs), (3) endothelial-derived activated MPs (EDAc-MPs), and (4) endothelial-derived apoptotic MPs (EDAp-MPs), were measured just after the patient was enrolled into the study using flow cytometry. (hindawi.com)
  • Microparticles were identified by flow cytometry. (nih.gov)
  • The EPCs (CD133 + /KDR + , CD34 + /KDR + ) and endothelial microparticles (EMPs: CD31 + /CD41 − , CD144 + , CD62e + ) were determined in blood using flow cytometry. (onlinejacc.org)
  • Microparticles were produced by activating human neutrophils with a calcium ionophore and characterized by flow cytometry and transmission and scanning electron microscopy. (biomedcentral.com)
  • Neutrophil microparticles-induced injuries and morphological alterations in human umbilical vein endothelial cells (HUVECs) were evaluated by microscopy and flow cytometry. (biomedcentral.com)
  • In this study, characterization of PMPs and cell lines were done by flow cytometry. (ac.ir)
  • RESEARCH DESIGN AND METHODS Levels and cellular origin of vitreous and plasma microparticles from control ( n = 26) and diabetic ( n = 104) patients were analyzed by flow cytometry, and their proangiogenic activity was assessed by in vitro thymidine incorporation and neovessel formation in subcutaneous Matrigel plugs in mice. (diabetesjournals.org)
  • By inducing ALI with intratracheal instillation of lipopolysaccharide (LPS) in C57BL6 mice, the authors found that MVs released into the injured alveolus were mainly from alveolar macrophages initially by flow cytometry, peaking at 1 hour and followed soon thereafter by MVs released by alveolar epithelial cells and neutrophils by 4 hours. (bmj.com)
  • Second, in early ALI following intratracheal LPS instillation, alveolar macrophages were the initial source of the pro-inflammatory MVs, followed soon thereafter by MVs released from alveolar epithelial cells and neutrophils, as determined by flow cytometry. (bmj.com)
  • Sarah E. Headland, Hefin R. Jones, Adelina S. V. D'Sa, Mauro Perretti & Lucy V. Norling Cutting-Edge Analysis of Extracellular Microparticles using ImageStreamX Imaging Flow Cytometry. (isac-net.org)
  • Analysis of antigen presenting cell derived exosomes, based on immuno‐magnetic isolation and flow cytometry. (currentprotocols.com)
  • AnnexinV-positive MP (TMP), MP derived from platelets (PMP), erythrocytes (ErMP), endothelial cells (EMP), leukocytes (LMP) and granulocytes (PNMP) were determined by flow cytometry. (diva-portal.org)
  • HUVECs (3 × 10 6 cells/condition) were treated with either high glucose-derived EMPs (hgEMPs) or normal glucose-derived (ngEMPs) for 24 h and surface expression of E-selectin (CD62E-PE), ICAM-1 (CD54-FITC), VCAM-1 (CD106-APC) and PECAM-1 (CD31-BV) was assessed by flow cytometry and reported as mean fluorescent intensity (MFI). (springermedizin.de)
  • This study tested the hypothesis that circulating microparticles (MPs) are useful biomarkers for predicting one-year mortality in patients with end-stage non-small cell lung cancer (ES-NSCLC). (hindawi.com)
  • INTRODUCTION: Circulating cell-derived microparticles (MPs) have been implicated in several disease processes and elevated levels are found in many pathological conditions. (uzh.ch)
  • Circulating endothelial microparticles have been proposed as markers of endothelial injur. (bioportfolio.com)
  • Increased circulating cell-derived microparticle count is associated with recurrent implantation failure after IVF and embryo transfer. (semanticscholar.org)
  • Circulating microparticles: a marker of procoagulant state in normal pregnancy and pregnancy complicated by preeclampsia or intrauterine growth restriction. (semanticscholar.org)
  • Endothelium-dependent vasodilation, circulating levels of endothelial cell-derived microparticles and bone marrow-derived endothelial progenitor cells, and indices of nitric oxide inhibition are assessed before and 3, 40, 70 and 100 min after negative emotion induction. (bmj.com)
  • In addition to functional measures (ie, flow-mediated dilation), this study is also assessing cellular measures of endothelial function including circulating endothelial cell-derived microparticles and endothelial progenitor cells. (bmj.com)
  • We studied the effects of acute SHS exposure on the number and function of endothelial progenitor cells, plasma vascular endothelial growth factor, circulating endothelial microparticles, and flow-mediated vasodilation at serial time points until 24 h after exposure. (onlinejacc.org)
  • Circulating endothelial progenitor cells and cellular membrane microparticles in db/db diabetic mouse: possible implications in cerebral ischemic d. (nih.gov)
  • For determining the implications of circulating endothelial progenitor cells (cEPCs) and cellular membrane microparticles (MPs) in diabetic stroke, levels of EPCs, EPC-MPs, and endothelium-derived MPs (EMPs) and their correlations with blood glucose concentration, cerebral microvascular density (cMVD), and ischemic damage were investigated in type 2 diabetic db/db and db/+ (wild-type control) mice. (nih.gov)
  • The aim of this study was to assess the association between circulating cell-derived microparticles (MPs) and type 2 diabetes mellitus (T2DM). (figshare.com)
  • Circulating cell-derived microparticles (MPs) are sub-micronic phospholipid vesicles derived from cells of different types in response to various biological processes such as cellular activation and apoptosis. (insajournal.in)
  • It has been known that levels of circulating cell-derived microparticles (MP) are generally increased in inflammatory states, making them useful as biomarkers. (ebrary.net)
  • In this paper, we describe how the circulating blood cells - monocytes, platelets, neutrophils and their microparticles - co-operate in regulating the expression, availability and activity of monocyte-derived TF. (biochemsoctrans.org)
  • Endothelial microparticles are small vesicles that are released from endothelial cells and can be found circulating in the blood. (wikipedia.org)
  • Although circulating endothelial microparticles can be found in the blood of normal individuals, increased numbers of circulating endothelial microparticles have been identified in individuals with certain diseases, including hypertension and cardiovascular disorders, and pre-eclampsia and various forms of vasculitis. (wikipedia.org)
  • and reducing circulating levels of bone marrow-derived endothelial progenitor cells (EPCs), a marker of EC reparative capacity (Hypothesis 1c). (clinicaltrials.gov)
  • Compared to the neutral condition, the depressed mood and separately the anxiety recall tasks will acutely impair endothelium-dependent arterial vasodilation, increase circulating levels of EMPs, and reduce circulating levels of bone marrow-derived EPCs. (clinicaltrials.gov)
  • Since the description of Trousseau syndrome in 1865, various experimental and clinical studies have detailed the interaction of platelets with primary tumors and circulating metastatic tumor cells. (ahajournals.org)
  • 5,6 Taken together, these seminal observations suggest a robust interaction between circulating platelets and tumor cells. (ahajournals.org)
  • Circulating microparticles (MP) are involved in the pathogenesis of atherothrombotic disorders and are raised in individual with CVD. (diva-portal.org)
  • We analysed circulating (annexin V+) microparticles and their conjugates in resuscitated patients. (biomedcentral.com)
  • The endothelium is a monolayer of cells, called endothelial cells, that lines the interior of blood vessels, including arteries, veins and cardiac chambers, 1 acting as a protective layer between circulating blood and other tissues. (scielo.br)
  • 1 The yield of endothelial cells culture from peripheral blood represents an important step in research on circulating angiogenic cells, since these cells were firstly isolated for research purpose. (scielo.br)
  • Prognostic value of circulating levels of stem cell growth factor beta (SCGF beta) in patients with Chagas' disease and idiopathic dilated cardiomyopathy. (genscript.com)
  • Mesenchymal stem or stromal cells (MSCs) are multipotent progenitor cells, which can be isolated from many tissues, such as bone marrow, adipose tissue, synovium or Wharton's jelly [ 1 ]. (thno.org)
  • Brief exposure to a real-world level of SHS not only causes acute vascular injury as indicated by endothelial dysfunction and microparticle generation, but also leads to sustained changes of the vascular repair system with a mobilization of dysfunctional endothelial progenitor cells. (onlinejacc.org)
  • Objectives This study sought to analyze the effects of acute secondhand smoke (SHS) exposure on the number and function of endothelial progenitor cells (EPCs) over 24 h. (onlinejacc.org)
  • EVs are secreted by all cell types including stem/progenitor cells. (springer.com)
  • In both human and animal models, the use of stem/progenitor cells (such as bone marrow mesenchymal stromal cells) has been shown to promote the recovery of kidney diseases such as acute kidney injury and chronic kidney disease. (springer.com)
  • Stem/progenitor cell-derived extracellular vesicles are an important mechanism by which stem/progenitor cells might repair kidney injury. (springer.com)
  • 17 - 21 As an example, overexpressing stromal derived factor-1 (CXCL12), an important chemokine in cardiac development, protects the myocardium and sustains the repairing after myocardial infarction, 22 by promoting regeneration and angiogenesis through recruitment of progenitor cells. (ahajournals.org)
  • Characterization of mRNA and microRNA in human mast cell-derived exosomes and their transfer to other mast cells and blood CD34 progenitor cells. (ispub.com)
  • Perpetual epithelial renewal is facilitated by stem and progenitor cells that balance proliferation and differentiation signals to continuously replace terminally differentiated or dying cells. (jci.org)
  • The encoded protein is a secreted sulfated glycoprotein and functions as a growth factor for primitive hematopoietic progenitor cells. (genscript.com)
  • abstract = "Hollow core/shell carbon microparticles, denoted HCSC600, are synthesized from yeast cells by coating the cells with silica shells, and then pyrolyzing the silica-protected yeast cells at 600°C, and finally etching the silica shells off of the carbonized products. (elsevier.com)
  • Cosenza S, Toupet K, Maumus M, Luz-Crawford P, Blanc-Brude O, Jorgensen C, Noël D. Mesenchymal stem cells-derived exosomes are more immunosuppressive than microparticles in inflammatory arthritis. (thno.org)
  • This prompted us to compare the function of exosomes (Exos) and microparticles (MPs) isolated from MSCs and investigate their immunomodulatory function in arthritis. (thno.org)
  • Extracellular vesicles are classified into exosomes, microvesicles/microparticles, or apoptotic bodies, originating from different subcellular compartments. (mendeley.com)
  • Furthermore, extracellular vesicles from supernatants of H4B4*KLH-pulsed MoDC contained significantly more HLA-DR and KLH than those purified from control MoDC, and KLH was concentrated specifically in exosomes that were a uniquely effective source of Ag in standard T cell proliferation assays. (jimmunol.org)
  • In summary, we identify LAMP-2 as an endocytic receptor on human MoDC that routes cargo into unusual Ag processing pathways, which reduces surface expression of Ag-derived peptides while selectively enriching Ag within immunogenic exosomes. (jimmunol.org)
  • Once considered cellular debris or, more recently, as biomarkers of disease progression, extracellular vesicles (EVs), comprised of exosomes, microvesicles (MVs) and apoptotic bodies, released by living and dying eukaryotic cells are now recognised as important mediators of cellular communication and function. (bmj.com)
  • Exosomes are formed by invagination within a cell to create an intracellular vesicle called an endosome, or an endocytic vesicle. (wikipedia.org)
  • As a potential mechanism, Mackie et al found that CD34 Shh cells are able to deposit greater amount of Shh in exosomes and transfer them to other cell types, activating the Shh signaling pathway in recipient cells. (ahajournals.org)
  • Among all cell types, only CD34 + cells preferentially store Shh in exosomes. (ahajournals.org)
  • Likewise, it is not clear whether these exosomes or microparticles also transport other factors potentially modulating these processes. (ahajournals.org)
  • Exosomes are membrane vesicles of intracellular origin that are secreted from the cell by exocytosis. (isac-net.org)
  • Characterization and proteomic profiling of pancreatic cancer-derived serum exosomes. (abcam.com)
  • Exosomes harbor B cell targets in pancreatic adenocarcinoma and exert decoy function against complement-mediated cytotoxicity. (abcam.com)
  • Exosomes released by hepatocarcinoma cells endow adipocytes with tumor-promoting properties. (abcam.com)
  • Exosomes are nanoparticles (~100 nm diameter) released from cells, which can transfer small RNAs and mRNA via the extracellular environment to cells at distant sites. (izon.com)
  • Microvesicles (ectosomes, or microparticles) are a type of extracellular vesicle (EV) that are released from the cell membrane. (wikipedia.org)
  • In multicellular organisms, microvesicles and other EVs are found both in tissues (in the interstitial space between cells) and in many types of body fluids. (wikipedia.org)
  • Microvesicles play a role in intercellular communication and can transport molecules such as mRNA, miRNA, and proteins between cells. (wikipedia.org)
  • Though initially dismissed as cellular debris, microvesicles may reflect the antigenic content of the cell of origin and have a role in cell signaling. (wikipedia.org)
  • Microvesicles may also remove misfolded proteins, cytotoxic agents and metabolic waste from the cell. (wikipedia.org)
  • Different cells can release microvesicles from the plasma membrane. (wikipedia.org)
  • Microvesicles are small, plasma membrane-derived particles that are released into the extracellular environment by the outward budding and fission of the plasma membrane. (wikipedia.org)
  • Ectosomes, also commonly referred to as microvesicles or microparticles, are shed from the plasma membrane. (isac-net.org)
  • Several different measurement approaches can be taken for the analysis of cell-derived microvesicles, and each has their advantages and disadvantages. (isac-net.org)
  • Because of the potential importance of microvesicles as diagnostic or prognostic markers, there is significant interest in standardizing sample preparation and measurement approaches for cell-derived microvesicles. (isac-net.org)
  • To investigate this mechanism, we focused on tumor-derived microvesicles (TMV). (plos.org)
  • Recent studies report that crosstalk between tumor cells and stromal cells are important for tumor progression or acceleration of tumor malignancy by secreting microvesicles (MVs) [11] , [12] . (plos.org)
  • After activation, platelets release small vesicles, called microparticles or microvesicles. (ahajournals.org)
  • Microvesicles (MVs) are cell-derived vesicles which are of interest in a clinical setting, as they may be predictive of early signs of disease and/or of treatment progression. (izon.com)
  • Stable cell fate changes in marrow cellsinduced by lung-derived microvesicles. (ispub.com)
  • In this study, we evaluated the effects on matrix catabolic activity of AF cells by the extracellular endothelial microparticles (EMPs) and soluble protein factors (SUP fraction) produced from ECs. (elsevier.com)
  • Passage 1 human AF cells grown in monolayer cultures were treated for 72 h with 250 µg of EMPs or SUP fraction isolated from culture of the microvascular endothelial cell line, HEMC-I. Live-cell imaging revealed uptake of EMPs by AF cells. (elsevier.com)
  • RT-PCR analysis demonstrated increased mRNA expression of MMP-1 (50.3-fold), MMP-3 (4.5-fold) and MMP-13 (5.5-fold) in AF cell cultures treated with EMPs compared to untreated control. (elsevier.com)
  • Increased MMP expression is primarily due to EMP or SUP stimulation of AF cells since EMPs or SUP fraction alone contained negligible amount of MMPs. (elsevier.com)
  • Interestingly, MMP activity was elevated in AF cell cultures treated with EMPs but not with SUP. (elsevier.com)
  • This study revealed enhanced matrix catabolism as a molecular consequence of action of ECs on AF cells via EMPs, which might be expected during neo-angiogenesis of degenerating disc. (elsevier.com)
  • Two blood samples were drawn, one immediately and one 24 hours after return of spontaneous circulation (ROSC) to detect (annexin V+) monocyte-derived microparticles (MMPs) or procoagulant (annexin V+) platelet-derived microparticles (PMPs) and conjugates of endothelial-derived (annexin V+) microparticles (EMPs) with monocytes (EMP-MC) or platelets (EMP-PC). (biomedcentral.com)
  • The experimental aim of this study was to determine, in vitro, the effects of glucose-induced EMPs on endothelial cell expression of E-selectin, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 and platelet cell adhesion molecule-1 (PECAM-1). (springermedizin.de)
  • Hyperglycemic-derived EMPs induced significantly higher surface expression of E-selectin (2614 ± 132 vs. 2010 ± 204 MFI), ICAM-1 (2110 ± 81 vs. 1688 ± 152 MFI), VCAM-1 (3589 ± 431 vs. 2134 ± 386) and PECAM-1 (4237 ± 395 vs. 2525 ± 269 MFI) on endothelial cells than EMPs from normoglycemic conditions. (springermedizin.de)
  • In contrast, diagnosing vascular complications in patients undergoing SCT is challenging, and damage to endothelial cells is regarded as the common feature of these complications. (nature.com)
  • 7 , 8 In addition, these microparticles have more recently been shown to reflect in vitro cell stimulation, and testify to cellular activation and/or tissue degeneration occurring in vivo under a variety of pathophysiologic circumstances. (nature.com)
  • This study aimed to investigate whether KMSC-derived MPs show anti-fibrotic effects by ameliorating endothelial-to-mesenchymal transition (EndoMT) in human umbilical vein endothelial cells (HUVEC) in vitro and by preserving PTC in kidneys with unilateral ureteral obstruction (UUO) in vivo . (biomedcentral.com)
  • MPs isolated from the supernatants of KMSC were co-cultured with HUVEC to assess their in vitro biologic effects on endothelial cells. (biomedcentral.com)
  • KMSC-derived MPs significantly ameliorated EndoMT and improved in vitro proliferation of TGF-β1 treated HUVEC. (biomedcentral.com)
  • Detection of fetal DNA and RNA in placenta-derived syncytiotrophoblast microparticles generated in vitro. (semanticscholar.org)
  • EPC generation and functions were evaluated by in vitro cell cultures. (nih.gov)
  • The in vitro treatment of tumor cells with platelet [Plt] causes inhibition of tumor cell growth , although mechanism of this effect is not clear yet. (bvsalud.org)
  • Vaccines composed of DCs pulsed with the supernatants of chemically stressed CRC cells induced a more significant IFN-γ-producing Th1 response both in vitro and in vivo . (nature.com)
  • Through experiments performed in vivo and in vitro , DAMPs induced by chemical drugs have been demonstrated to stimulate dendritic cells (DCs) to induce an anti-cancer immune response. (nature.com)
  • Using IBD clinical samples, and in vitro and in vivo injury models, we show that PMN-derived miR-23a and miR-155 promote accumulation of double-strand breaks (DSBs) by inducing lamin B1-dependent replication fork collapse and inhibition of homologous recombination (HR) by targeting HR-regulator RAD51. (jci.org)
  • 4 Tumor cells that aggregate platelets in vitro produce more lung metastases than tumor cells lacking such ability, illustrating the platelet-activating potential of some tumor cells. (ahajournals.org)
  • In vitro, the release of microparticles (MPs) from platelets [platelet-derived MPs (PMPs)] has been associated with the secretion response ( 4 ). (aaccjnls.org)
  • Inflammation determines the pro-adhesive properties of high extracellular d -glucose in human endothelial cells in vitro and rat microvessels in vivo. (springermedizin.de)
  • 2 The methods include in vitro analysis, such as culture of endothelial cells, and in vivo analysis, such as flow-mediated dilation (FMD), venous occlusion plethysmography (VOP) and measurement of serum markers. (scielo.br)
  • Culture of endothelial cells has been widely described in the literature, especially in studies on the effect of oxidative stress and inflammation on mobilization and proliferation of these cells in vitro . (scielo.br)
  • The ability of microparticles to support coagulation was determined in normal plasma. (nih.gov)
  • Shimizu, M. Microparticles as biomarkers of blood coagulation in cancer. (eurekaselect.com)
  • Interactions of platelets, coagulation, and tumor cells in tumorigenesis. (ahajournals.org)
  • Schematic diagram showing the interplay among various proteins: platelet receptors, coagulation proteins, and tumor cells interacting in the process of tumorigenesis. (ahajournals.org)
  • Here, we investigate the role of microparticles (MP), a member of the extracellular vesicle family, in the exchange of pro-inflammatory molecules between brain immune cells, as well as their transfer to the systemic circulation, as key pathways of inflammation propagation following brain trauma. (biomedcentral.com)
  • PDMPs have been associated with TTP, and endothelial cells and monocytes can develop the same microparticles. (nature.com)
  • Methods:Human umbilical vein endothelial cells and U937 monocytes were stimulated with TNF-α to produce MPs. (le.ac.uk)
  • 2 Injuries to the endothelium trigger an inflammatory response with participation of several cell types - lymphocytes, monocytes, platelets and smooth muscle cells 5 - culminating in endothelial cell dysfunction, stiffness of vessel wall and atherosclerotic plaque formation. (scielo.br)
  • In response to damage, epithelial wounds repair by a series of events that integrate epithelial responses with those of resident and infiltrating immune cells including neutrophils and monocytes/macrophages. (jci.org)
  • Synovial microparticles bound less annexin V (which binds to negatively charged phospholipids) than did plasma microparticles, exposed tissue factor, and supported thrombin generation via factor VII. (nih.gov)
  • Induction of plasma (TRAIL), TNFR-2, Fas ligand, and plasma microparticles after human immunodeficiency virus type 1 (HIV-1) transmission: implications for HIV-1 vaccine design. (semanticscholar.org)
  • In PDR, the endothelial microparticles ratio-but not that for platelet-was greater than 1.0, indicating local formation of endothelial microparticles from retinal vessels and permeation of platelet microparticles from plasma. (diabetesjournals.org)
  • 23 , 24 Notably, the vasoregenerative and angiogenic potential of angiogenic early outgrowth cells can be enhanced by the transfer of the CXCL12 receptor CXCR4 through platelet microparticles. (ahajournals.org)
  • Platelet microparticles are small in size (0.05 to 1 μm) with a defined plasma membrane and express selected platelet membrane and cellular proteins. (ahajournals.org)
  • There is increasing research on and clinical interest in the physiological role played by platelet microparticles (PMPs). (izon.com)
  • Mesenchymal stem cells (MSCs) release extracellular vesicles (EVs) that display a therapeutic effect in inflammatory disease models. (thno.org)
  • Cell-cell communication has proven to be even more complex than previously thought since the discovery that extracellular vesicles serve as containers of biological information on various pathophysiological settings. (mendeley.com)
  • More than simply cell dust, extracellular vesicles are capable of transferring biological information to neighboring cells and play an active role in inflammatory diseases, including atherosclerosis and angiogenesis. (mendeley.com)
  • Here, this review will discuss the latest advances concerning the application potential of stem/progenitor cell-derived extracellular vesicles in renal diseases, including the aspects as follows: anti-inflammatory, proliferation-promoting and anti-apoptotic, proangiogenic, antifibrotic and renal cancer progression-promoting. (springer.com)
  • Therefore, stem/progenitor cell-derived extracellular vesicles may be a promising treatment tool for renal diseases. (springer.com)
  • Extracellular vesicles (EVs) are a heterogeneous class of membrane-bound cell-derived structures that can engage in intercellular communication via the trafficking of bioactive molecules between cells and tissues. (mdpi.com)
  • This review outlines the mechanisms and functions of dying-cell-derived EVs and their ability to drive inflammation during various modes of cell death, whilst reflecting on the challenges and knowledge gaps in investigating this subgenre of extracellular vesicles research. (mdpi.com)
  • Small Extracellular Vesicles in Rat Serum Contain Astrocyte-Derived Protein Biomarkers of Repetitive Stress. (abcam.com)
  • Priming of dendritic cells by DNA-containing extracellular vesicles from activated T cells through antigen-driven contacts. (abcam.com)
  • Extracellular vesicles (EVs) are the collective term for the various vesicles that are released by cells into the extracellular space. (izon.com)
  • Urinary extracellular vesicles (uEVs) are released by cells throughout the nephron and contain biomolecules from their cells of origin. (izon.com)
  • In this proof-of-principle study a label-free optical sensor is demonstrated to monitor the surface adhesion of extracellular vesicles secreted by live cells on to various extracellular matrix proteins. (izon.com)
  • Low molecular weight heparin (LMWH)/protamine (P) nano/micro particles (N/MPs) (LMWH/P N/MPs) were applied as carriers for heparin-binding growth factors (GFs) and for adhesive cells including adipose-derived stromal cells (ADSCs) and bone marrow-derived mesenchymal stem cells (BMSCs). (mdpi.com)
  • 4 - 10 Among all, autologous bone marrow-derived CD34 + stem cells demonstrate a major benefit, showing favorable impact on angiogenic growth, without the toxicity and inflammatory response observed in nonautologous cell-based therapy. (ahajournals.org)
  • Washing samples results in decreased AnnV+MPs (P=0.002) and platelet-derived MPs (PMPs) (P=0.002). (uzh.ch)
  • Membrane-derived microparticles (PMPs) are produced from platelets during activation, storage, and apoptosis. (ac.ir)
  • In this study, the ability of PMPs to deliver CXCR4 molecule to CXCR4-null targets (Daudi, K562 and U937 cell line) was evaluated and the different concentrations of PMPs were examined to transfer CXCR4. (ac.ir)
  • PMPs in 7 different concentrations were added to cell culture plates and incubated for 1 hour at 37ºc and 5% CO2. (ac.ir)
  • PMPs in different concentrations can transfer CXCR4 to target cells. (ac.ir)
  • Also, the increase of PMPs concentration up to 250µg/mL can increase the CXCR4 presence on null cells. (ac.ir)
  • Platelet-derived microparticles (PMPs) are generally considered a marker of platelet activation in cardiovascular disease. (aaccjnls.org)
  • Microparticles (MPs) are small vesicles, which typically range in size from 0.1 to 1.5 μm [ 9 ], shed from the plasma membrane into the extracellular space by most eukaryotic cells undergoing activation or apoptosis. (biomedcentral.com)
  • The microparticles also expressed CD66b, CD62L and myeloperoxidase, which are all commonly expressed on the surface of neutrophils, as well as exposition of phosphatidylserine. (biomedcentral.com)
  • When instilled intratracheally in naive mice, MVs isolated from macrophages primed with LPS induced ALI with an increase in influx of neutrophils and cytokines/chemokines (eg, KC), in lung protein permeability and in the expression of ICAM-1 on alveolar epithelial type I and II cells (Figure 8). (bmj.com)
  • In one embodiment, the invention relates to a particle or cell separation device that separates specific cell types such as neutrophils or stem cells or particles from bodily fluids such as platelets and other blood products such as red cells and plasma. (google.com)
  • Specifically, the neutrophils (a type of white blood cell also known as polymorphonuclear cells) contain a variety of powerful enzymes that can cause tissue inflammation. (google.com)
  • outline a mechanism whereby neutrophils control epithelial fitness and genomic instability via delivery of miR-23a-and miR-155-containing microparticles. (jci.org)
  • As the first immune cell recruited to sites of inflammation, neutrophils and their cargo are crucial for shaping mucosal responses to infection, injury, and repair. (jci.org)
  • Fcγ receptor mediated phagocytosis includes formation of protrusions of the cell called a 'phagocytic cup' and activates an oxidative burst in neutrophils. (wikipedia.org)
  • However, the supernatants of chemically stressed CRC cells failed to induce phenotypic maturation and cytokine production in TLR4-deficient DCs, indicating an essential role of TLR4 in DAMP-induced DC maturation and activation. (nature.com)
  • Furthermore, pulsing with the supernatants of chemically stressed CRC cells did not efficiently induce an IFN-γ-producing Th1 response in TLR4-deficient DCs. (nature.com)
  • Moreover, the Shh exosomal transfer is able to induce Shh specific signaling not only in endothelial cells, but also in fibroblasts. (ahajournals.org)
  • Leicester Research Archive: Monocyte- and Endothelial-Derived Microparticles Induce an Inflammatory Phenotype in Human Podocytes. (le.ac.uk)
  • Monocyte- and Endothelial-Derived Microparticles Induce an Inflammatory Phenotype in Human Podocytes. (le.ac.uk)
  • Microparticles (MPs), small membrane fragments shed from various cell types, have been implicated in thrombosis, inflammation, and endothelial dysfunction. (thejns.org)
  • Those subtypes closely associated with thrombosis and endothelial dysfunction, for example, CD145+(TF)-associated MPs and CD146+(endothelial cell)-associated MPs, had the most durable response and demonstrated a significant negative correlation with radiographic infarction at 14 days after SAH. (thejns.org)
  • The endothelial microparticles in some of these disease states have been shown to have arrays of cell surface molecules reflecting a state of endothelial dysfunction. (wikipedia.org)
  • At the beginning of atherosclerotic plaque formation, endothelial dysfunction is characterized by increased expression and release of adhesion molecules, including endothelial selectin (E-selectin), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). (scielo.br)
  • Induction of apoptosis has been proposed as a mechanism of Plt effects on tumor cells . (bvsalud.org)
  • however, the role of DAMPs released from chemical drug-treated tumor cells in the activation of the immune response needs to be further elucidated. (nature.com)
  • Efficiently killing tumor cells with chemical drugs while enhancing induction of the anti-tumor immune response is becoming increasingly important. (nature.com)
  • Here we showed that TMV isolated from tumor cells were taken up by NEC through endocytosis. (plos.org)
  • Tumor blood vessels provide nutrition and oxygen, promote tumor progression, and serve as gatekeepers for tumor cells to metastasize to other organs. (plos.org)
  • MVs are released from various tumor cells and some progenitors of differentiated cells [14] . (plos.org)
  • Observations have suggested that platelets not only augment the growth of primary tumors via angiogenesis but endow tumor cells physical and mechanical support to evade the immune system and extravasate to secondary organs, the basis of metastatic disease. (ahajournals.org)
  • 3 Others have shown that intravenous injection of some tumor cells may cause significant thrombocytopenia (50% to 70%) in mice. (ahajournals.org)
  • We found that myofibroblasts triggered cues in the tumor microenvironment, which led to induction of CXCL4L1 in tumor cells. (aacrjournals.org)
  • Human tumor cells stained for DAPI (blue), cytochrome c (red), and CHCHD4 (green). (jci.org)
  • The aim of this work was to use MSMPs to deliver viral specific MHC class I restricted epitopes into human antigen presenting cells (monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T lymphocytes. (hindawi.com)
  • The most powerful antigen presenting cells are dendritic cells (DCs), which bridge innate and adaptive immunity and are capable of initiating a primary immune response by activating naïve T cells [ 10 ]. (hindawi.com)
  • In this study we identify LAMP-1 (CD107a) and LAMP-2 (CD107b) on the surface of human monocyte-derived dendritic cells (MoDC) and show only LAMP-2 is internalized after ligation by specific Abs, including H4B4, and traffics rapidly but transiently to the MHC class II loading compartment, as does Ag conjugated to H4B4. (jimmunol.org)
  • One of the major challenges towards increasing the vaccine efficacy has been to develop maturation procedures that produce highly immunogenic dendritic cells that also demonstrate strong migratory skills. (dtu.dk)
  • To test the migratory skills of the dendritic cells induced by the different procedures we present a versatile and easy to use chip integrated migration platform. (dtu.dk)
  • Dendritic cells seeded into the polymer chip in a concentration gradient of the chemoattractant CCL21 efficiently negotiated the microporous maze structure for pore sizes of 8x8 μm or larger. (dtu.dk)
  • Linear microchannels with diameters from 10 μm to 20 μm were also produced and simultaneous observations of dendritic cells migrating in the confined channels and in the fibrillar collagen were performed. (dtu.dk)
  • To more precisely mimic the mechanical and chemical properties of the tissue traversed by the dendritic cells we also present a poly (ethylene glycol) diacrylate (PEGDA) based strategy to fabricate soft 3D hydrogel scaffolds. (dtu.dk)
  • Dendritic cells also reside in tissues and ingest pathogens by phagocytosis. (wikipedia.org)
  • We measured and compared the levels of microparticles and soluble factors in patients undergoing allogeneic SCT. (nature.com)
  • Table 1 shows changes in the soluble factors and microparticles. (nature.com)
  • By mimicking pathogen dimensions, microparticles are more prone to be phagocyted by APCs than soluble antigen. (hindawi.com)
  • Because of alternative splicing TF is expressed in 3 mRNA splice variants: full-length (fl)TF, alternatively spliced (as)TF, and a third variant named TF-A. Translation of flTF and asTF mRNA splice variants leads to the generation of the flTF isoform, a membrane-bound and highly procoagulant protein (8), and soluble asTF with low prothrombogenic potential but strong proangiogenic, cell proliferation-facilitating, and prosurvival activities (2, 9, 10). (thefreelibrary.com)
  • Cellular microparticles are fragments that shed almost spontaneously from the plasma membrane blebs of virtually all cell types when submitted to a number of stress conditions. (nature.com)
  • 7 , 8 Therefore, there is a possibility that cellular microparticles exhibit the dynamic change after SCT. (nature.com)
  • To determine the cellular origin of synovial microparticles, their procoagulant properties, and their relationship to local hypercoagulation. (nih.gov)
  • Microparticles as regulators of inflammation: novel players of cellular crosstalk in the rheumatic diseases. (semanticscholar.org)
  • Microparticles as mediators of cellular cross-talk in inflammatory disease. (semanticscholar.org)
  • Shedding of microparticles by myofibroblasts as mediator of cellular cross-talk during normal wound healing. (semanticscholar.org)
  • Microparticles have been identified not only in human plasma but also in other tissues with high cellular activation, inflammation, or apoptosis, such as human atherosclerotic plaques or synovial fluid in rheumatoid arthritis ( 13 , 14 ). (diabetesjournals.org)
  • Microvesicle budding takes place at unique locations on the cell membrane that are enriched with specific lipids and proteins reflecting their cellular origin. (wikipedia.org)
  • 1 , 2 Therefore, in the last decade, the efforts were focused on studying the complex molecular and cellular mechanisms contributing to improved myocardial remodeling and function after stem cell transplantation. (ahajournals.org)
  • Communication between dying cells and their environment is a critical process that promotes tissue homeostasis during normal cellular turnover, whilst during disease settings, it can contribute to inflammation through the release of intracellular factors. (mdpi.com)
  • Although once seen as inert cellular debris, a plethora of literature spanning numerous fields of medicine now describes EV-mediated cargo transfer that can directly modulate the functions of target cells and tissues [ 7 , 8 , 9 , 10 ]. (mdpi.com)
  • This emerging field pertains to nano & microparticles that carry proteins and RNA, both of which serve important cellular functions, and is likely to be an important player in the fast evolving field of nanomedicine. (ispub.com)
  • Although only a simple monolayer, the healthy endothelium is optimally placed and is able to respond to physical and chemical signals by production of a wide range of factors that regulate vascular tone, cellular adhesion, thromboresistance, smooth muscle cell proliferation, and vessel wall inflammation. (ahajournals.org)
  • Targeted inhibition of miR-23a and miR-155 in cultured intestinal epithelial cells and in acutely injured mucosa decreased the detrimental effects of PMNs and enhanced tissue healing responses, suggesting that this approach can be used in therapies aimed at resolution of inflammation, in wound healing, and potentially to prevent neoplasia. (jci.org)
  • Rapid self-renewal also supports epithelial cells' essential role in barrier regulation and wound repair. (jci.org)
  • Microparticles (MPs) derived from kidney-derived mesenchymal stem cells (KMSCs) have recently been reported to ameliorate rarefaction of peritubular capillaries (PTC) in ischemic kidneys via delivery of proangiogenic effectors. (biomedcentral.com)
  • Recently, myofibroblasts have been shown to rise from endothelial cells via endothelial-to-mesenchymal transition (EndoMT) induced by the transforming growth factor-β (TGF-β) family of regulatory polypeptides in experimentally induced fibrotic diseases. (biomedcentral.com)
  • The successful development of an advanced therapy medicinal product (ATMP) made of mesenchymal stem cells (MSCs) for the treatment of articular cartilage based on actual EU regulations and clinical recommendations lie within the realm. (selectbiosciences.com)
  • 3,4 They were demonstrated to be the active component of mesenchymal stem cell therapy and thus have wide potential therapeutic uses. (ispub.com)
  • The administration of G-CSF can affect serum cytokine levels of these patients, 3 resulting in the generation of procoagulant factors such as platelet-derived microparticles (PDMP). (nature.com)
  • Sterile inflammation of endothelial cell-derived apoptotic bodies is mediated by interleukin-1α. (semanticscholar.org)
  • Numerous studies now indicate that microparticles have biological activities and may be involved in thrombosis, cell inflammation, angiogenesis and cell-to-cell communication ( 5 - 12 ). (diabetesjournals.org)
  • Avenanthramides attenuate inflammation and atrophy in muscle cells. (abcam.com)
  • Resuscitated patients show substantially increased levels of different (annexin V+) microparticles and their conjugates immediately and 24 hours after cardiopulmonary resuscitation, suggesting an early onset of inflammation, an ongoing endothelial activation and a procoagulatory state. (biomedcentral.com)
  • The authors provide extensive updates on the latest findings concerning the mechanisms of inflammation and introduce such new concepts and methodologies as "endothelial and leukocyte microparticles" and "gene microarray technology" to help explain important links between the central nervous system (CNS) and general inflammatory processes. (springer.com)
  • Microparticles are a heterogeneous population of membrane-coated vesicles which can be released from virtually all cell types during activation or apoptosis. (semanticscholar.org)
  • Microparticles are submicron membrane vesicles with biological activities, released following cell activation or apoptosis. (diabetesjournals.org)
  • Additionally, microparticles of CPR patients may contribute to endothelial apoptosis. (biomedcentral.com)
  • Shedding of membrane microparticles is a physiological process that accompanies cell growth and activation and that is enhanced by cytokines, reactive oxygen species, activation of apoptotic pathways, or increases in intracellular calcium leading to cytoskeleton reorganization. (diabetesjournals.org)
  • It involves virtually all renal intrinsic and infiltrating cells and is characterized by alterations in their phenotype and accumulation of excessive extracellular matrix proteins [ 1 - 4 ]. (biomedcentral.com)
  • The encapsulation of recombinant proteins in biocompatible and biodegradable nano- and microparticles is emerging as a promising approach to boost their immunogenicity by passively targeting them to antigen presenting cells (APCs) [ 7 - 9 ]. (hindawi.com)
  • Upon activation neutrophil releases microparticles - small plasma membrane vesicles that contain cell surface proteins and cytoplasmic matter, with biological activities. (biomedcentral.com)
  • Tumor-derived MVs (TMV) contain bioactive molecules such as microRNAs (miRNA), mRNAs, and/or proteins inside themselves [15] . (plos.org)
  • EVs are a heterogeneous class of membrane-bound cell-derived structures produced by all eukaryotic cells that can harbour bioactive molecules, including nucleic acids, lipids and proteins, derived from their parental cells [ 1 , 6 ]. (mdpi.com)
  • 1 µm) and the expression of surface markers (CD66b for neutrophil-derived MPs and CD41b for platelet-derived MPs). (scielo.br)
  • In this study we investigated the potential role of myeloperoxidase in the endothelial cell injury caused by neutrophil-derived microparticles. (biomedcentral.com)
  • This mechanism of neutrophil-derived microRNA shuttling opens up new therapeutic potential to enhance epithelial healing and limit mucosal injury. (jci.org)
  • Membrane microparticles are submicron fragments of membrane vesicles shed from various cell types. (bioportfolio.com)
  • Microparticles are submicron membrane vesicles shed from the cell surface of both healthy and damaged cells ( 4 ). (diabetesjournals.org)
  • Plasma from patients and healthy controls contained comparable numbers of microparticles, which originated from platelets and erythrocytes. (nih.gov)
  • We identified what we believe is a new, ROS-independent mechanism whereby activated tissue-infiltrating PMNs release microparticles armed with proinflammatory microRNAs (miR-23a and miR-155). (jci.org)
  • Submicron cell-derived microparticles (MP) are important biological messengers. (ovid.com)
  • Therefore, we aimed to detect the presence neutrophil (CD66b+) and platelet (CD41b+) derived microparticles in gingival crevicular fluid from individuals having periodontitis aggravated by type 2 diabetes. (scielo.br)
  • In conclusion, this study reported the presence of neutrophil and platelet derived microparticles in gingival crevicular fluid from individuals having severe periodontitis and type 2 diabetes. (scielo.br)
  • Microparticle content of platelet concentrates is predicted by donor microparticles and is altered by production methods and stress. (semanticscholar.org)
  • The microparticles are characterized by various methods, including scanning electron microscopy, transmission electron microscopy, nitrogen gas adsorption/desorption and X-ray photoelectron spectroscopy. (elsevier.com)
  • However, they also highlight the need for new, more sensitive methods for measuring cell-derived vesicles. (isac-net.org)
  • The lysosome-associated membrane protein (LAMP) family includes the dendritic cell endocytic receptors DC-LAMP and CD68, as well as LAMP-1 and LAMP-2. (jimmunol.org)
  • The membrane of the endothelial microparticle contains receptors and other cell surface molecules which enable the identification of the endothelial origin of the microparticle, and allow it to be distinguished from microparticles from other cells, such as platelets. (wikipedia.org)
  • Engaging multiple receptors with bispecific biologics offers the potential to improve upon single-agent checkpoint blockade and promises to be the next generation of immunotherapy with preclinical, translational and clinical studies on using bispecific antibodies for dual blockade of checkpoint targets, T-cell-redirecting bispecific biologics, overcoming T-cell exhaustion, as well as strategies to improve efficacy and reduce toxicity, and engineer the next generation of bi- and multi-specific biologics. (genomicglossaries.com)
  • Among these are receptors that recognise the Fc part of bound IgG antibodies, deposited complement or receptors, that recognise other opsonins of cell or plasma origin. (wikipedia.org)
  • Monocyte-derived microparticles (MMPs) contain organized membrane receptors including ß2-integrins, like Mac-1 (CD11b/CD18). (biomedcentral.com)
  • We tested the hypothesis that proangiogenic microparticles accumulate in vitreous fluid in diabetic retinopathy. (diabetesjournals.org)
  • Exos and MPs from MSCs inhibited T lymphocyte proliferation in a dose-dependent manner and decreased the percentage of CD4 + and CD8 + T cell subsets. (thno.org)
  • One major role of MSCs is to suppress proliferation and function of cells of both innate and adaptive immunity [ 2 ]. (thno.org)
  • Furthermore, three-dimensional (3D) cultures of cells including ADSCs and BMSCs using plasma-medium gel with LMWH/P N/MPs exhibited efficient cell proliferation. (mdpi.com)
  • Isolated vitreous microparticles stimulated by 1.6-fold endothelial proliferation and increased new vessel formation in mice. (diabetesjournals.org)
  • KLH) and recall (Bet v 1) Ags (H4B4*KLH and H4B4*Bet v 1) induced significantly less CD4 cell proliferation than pulsing with native Ag or Ag conjugated to control mAb (ISO*KLH and ISO*Bet v 1). (jimmunol.org)
  • Paradoxically, MoDC pulsed with H4B4*KLH, but not the other KLH preparations, induced robust proliferation of CD4 cells separated from them by a transwell membrane, indicating factors in the supernatant were responsible. (jimmunol.org)
  • CXCL4L1 has been shown to be more potent than CXCL4 at inhibiting cell proliferation and migration ( 2, 3 ). (aacrjournals.org)
  • In contrast, FIXa, which is also generated by the TF-FVIIa complex in isolation or on TF-bearing cells, is more stable compared with FXa, allowing time for transport from TF-bearing cells to e.g. activated platelets, without significant inactivation by plasma inhibitors. (biochemsoctrans.org)
  • Rapid isolation of two distinct plasma membrane microparticle populations for biomarker discovery and assay. (ispub.com)
  • PMP can transfer some adhesion molecules such as CXCR4 to CXCR4-negative cells. (ac.ir)
  • CXCR4-negative cell lines (1×105 cells/ml) were cultured in RPMI1640 with 10% FBS and 1% antibiotic. (ac.ir)
  • The presence of CXCR4 on cells was analyzed by flowcytometry. (ac.ir)
  • The CD142+(tissue factor [TF])-associated MPs and CD146+(endothelial cell)-associated MPs were significantly elevated throughout the study period. (thejns.org)
  • Although MSCs can prevent arthritis, the role of MSCs-derived EVs has never been reported in rheumatoid arthritis. (thno.org)
  • Therefore, endothelial microparticles may be useful as an indicator or index of the functional state of the endothelium in disease, and may potentially play key roles in the pathogenesis of certain diseases, including rheumatoid arthritis. (wikipedia.org)
  • Cell-derived microparticles (MPs) have been described as vital contributors to the inflammatory process. (scielo.br)
  • Both MSCs-derived MPs and Exos exerted an anti-inflammatory role on T and B lymphocytes independently of MSCs priming. (thno.org)
  • Our work is the first demonstration of the therapeutic potential of MSCs-derived EVs in inflammatory arthritis. (thno.org)
  • Renal tubulointerstitial damage and inflammatory cell infiltration were examined with Masson's trichrome, F4/80 and α-smooth muscle actin (α-SMA) staining and PTC rarefaction index was determined by CD31 staining. (biomedcentral.com)
  • Furthermore, administration of KMSC-derived MPs inhibited inflammatory cell infiltration as well as tubulointerstitial fibrosis in UUO mice as demonstrated by decreased F4/80 and α-SMA-positive cells and Masson's trichrome staining, respectively. (biomedcentral.com)
  • 2) the effects of SCA/AA-RBC-MPs on human aortic endothelial cell (HAEC) inflammatory phenotype and TLR4 pathway. (frontiersin.org)
  • OBJECTIVE To assess current studies on the relationship between cell-derived microparticles (cMP) and recurrent miscarriages (RM) and pre-eclampsia (PE), and review the relationships between cMP and inflammatory and clot pathways, antiphospholipid antibodies (aPL), cytokines, and pregnancy complications. (semanticscholar.org)
  • Due to their ability to independently initiate inflammatory responses, MP derived from activated microglia may provide a potential therapeutic target for other neurological disorders in which neuroinflammation may be a contributing factor. (biomedcentral.com)
  • 4-6 In the current issue of Thorax , Soni et al 7 studied the temporal pattern of EV release in early ALI and found that MVs from alveolar macrophages were the predominant source and played a significant role as potent initiators of the inflammatory response in part by the transfer of its cargo, the cytokine tumour necrosis factor (TNF), to target cells. (bmj.com)
  • In experiments to study the mechanisms of MVs, the authors found that MVs released by primary cultures of mouse macrophages primed with LPS to obtain a pro-inflammatory phenotype also induced ICAM-1 expression in MLE-12 cells, which was dependent on the TNF cargo of the MVs. (bmj.com)
  • To fully understand the complex mechanisms mediating the recovery of the heart after an ischemic insult, one needs to consider not only effects on cells directly involved, but also collateral effects on the inflammatory reaction and the paracrine secretion of remote cells. (ahajournals.org)
  • In particular, during inflammatory settings such as acute tissue injury, infection and autoimmunity, the EV-mediated transfer of proinflammatory cargo from dying cells is an important process that can elicit profound proinflammatory effects in recipient cells and tissues. (mdpi.com)
  • Improved understanding of crosstalk between epithelial and immune cells during wound repair is necessary for development of better pro-resolving strategies to treat debilitating complications of disorders ranging from inflammatory bowel disease to diabetes. (jci.org)
  • Stem/progenitor cell-derived EVs have been identified to exert immunomodulatory effects on target cells through transferring protein molecules as well as regulatory effects on the phenotype of target cells through fusion with the target cells membrane and/or through direct endocytosis by target cells to transfer nucleic acid substances (such as mRNA, miRNA) to the target cells. (springer.com)
  • 8 The phenotype of the cells may determine the functional effects of the released MVs. (bmj.com)
  • Numerous diseases, recently reported to associate with elevated microvesicle/microparticle (MP) counts, have also long been known to be characterized by accelerated immune complex (IC) formation. (nih.gov)
  • Direct detection of activated platelets and platelet‐derived microparticles in humans. (currentprotocols.com)
  • The development of cell-based therapy for clinical studies raises the question whether the application of cell-based products to humans is safe. (selectbiosciences.com)
  • Prostacyclin, derived by the action of the cyclooxygenase system, is another endothelium-derived vasodilator that acts independently of NO. 11 Although it may contribute to some of the other regulatory roles of the endothelium, it appears to have a more limited role in the maintenance of vasodilator tone in humans. (ahajournals.org)
  • Human umbilical vein endothelial cells (HUVECs) were cultured (3rd passage) and plated in 6-well plates at a density of 5.0 × 10 5 cells/condition. (springermedizin.de)
  • Tiotropium inhibits proinflammatory microparticle generation by human bronchial and endothelial cells. (nih.gov)
  • Microparticles have recently emerged as a thrombotic risk marker with a potential role in determining which patients are at greatest risk for developing thrombosis. (bioportfolio.com)
  • CONCLUSIONS The present study demonstrates that vitreous fluid contains shed membrane microparticles of endothelial, platelet, and retinal origin. (diabetesjournals.org)
  • This hypothesis is based on the relatively rapid inactivation of the FXa formed on TF-bearing cells when it is released into the plasma. (biochemsoctrans.org)
  • Extracellular membrane vesicles generated by the shedding of CELL MEMBRANES blebs. (bioportfolio.com)
  • Leukocyte-derived MPs may also have a predictive role, although the studies regarding these are still limited. (eurekaselect.com)
  • There is an associated increase in platelet and leukocyte-derived microparticles. (bloodjournal.org)
  • Although stem cell transplantation (SCT) is being used for hematopoietic reconstitution following high-dose chemotherapy for malignancy, it involves some serious transplant-related complications. (nature.com)
  • 11 , 12 However, insufficient number of donor stem cells, low cell viability, and inefficient expansion techniques limit the effects of this therapy. (ahajournals.org)
  • Because low viability and inefficient expansion are important limitations of stem-cell therapies, 1 enhancing stem-cell survival in the hostile environment is the main goal of current cell-based therapy. (ahajournals.org)
  • 19 Genetic reprogramming of the transplanted stem cells allows them to serve as a source of growth factors, initiating intracrine, autocrine, and paracrine effects and augmenting the activity of endogenous cells. (ahajournals.org)
  • This dual approach, using stem-cell transplantation and genetic engineering to potentiate stem cell activity for myocardial repair, seems to be a reliable method and is currently successfully used in many experimental settings. (ahajournals.org)
  • Furthermore, EVs are increasingly recognised as potential diagnostic tools, serving as biomarkers for disease when isolated from biological fluids [ 11 , 12 , 13 , 14 ], whilst the therapeutic applications of stem-cell-derived EVs have demonstrated promising regenerative and immunomodulatory effects across a number of diseases [ 15 , 16 , 17 ]. (mdpi.com)
  • The presentation illustrates the legal gateways to the European market for (stem) cell based products from (basic) research to market approval. (selectbiosciences.com)
  • Experimental to clinical transplantation of pluripotent stem cells for cell therapy in retinal disease. (selectbiosciences.com)
  • The transformation of autologous adipose stem cells in a 3-dimensional osteogenic graft can open a new way for surgical reconstruction of large bone defect (after tumour resection and pseudarthrosis) with minimal invasive procedure. (selectbiosciences.com)
  • The articles in this dermatology Review Series demonstrate recent progress in understanding the cell biology and molecular pathophysiology of the epidermis and hair follicles, which harbor keratinocyte and melanocyte stem cells. (jci.org)
  • Bauer, N.N. On the origin of microparticles: From "platelet dust" to mediators of intercellular communication. (eurekaselect.com)
  • Star, R.A. Microparticles: markers and mediators of sepsis-induced microvascular dysfunction, immunosuppression, and AKI. (eurekaselect.com)