Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.
Relating to the size of solids.
Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.
A biocompatible polymer used as a surgical suture material.
The preparation, mixing, and assembling of a drug. (From Remington, The Science and Practice of Pharmacy, 19th ed, p1814)
Forms to which substances are incorporated to improve the delivery and the effectiveness of drugs. Drug carriers are used in drug-delivery systems such as the controlled-release technology to prolong in vivo drug actions, decrease drug metabolism, and reduce drug toxicity. Carriers are also used in designs to increase the effectiveness of drug delivery to the target sites of pharmacological actions. Liposomes, albumin microspheres, soluble synthetic polymers, DNA complexes, protein-drug conjugates, and carrier erythrocytes among others have been employed as biodegradable drug carriers.
A polyester used for absorbable sutures & surgical mesh, especially in ophthalmic surgery. 2-Hydroxy-propanoic acid polymer with polymerized hydroxyacetic acid, which forms 3,6-dimethyl-1,4-dioxane-dione polymer with 1,4-dioxane-2,5-dione copolymer of molecular weight about 80,000 daltons.
Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.
Deacetylated CHITIN, a linear polysaccharide of deacetylated beta-1,4-D-glucosamine. It is used in HYDROGEL and to treat WOUNDS.
Dosage forms of a drug that act over a period of time by controlled-release processes or technology.
A normal intermediate in the fermentation (oxidation, metabolism) of sugar. The concentrated form is used internally to prevent gastrointestinal fermentation. (From Stedman, 26th ed)
Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.
Chemistry dealing with the composition and preparation of agents having PHARMACOLOGIC ACTIONS or diagnostic use.
Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.
Structures which are part of the CELL MEMBRANE or have cell membrane as a major part of their structure.
The application of scientific knowledge or technology to pharmacy and the pharmaceutical industry. It includes methods, techniques, and instrumentation in the manufacture, preparation, compounding, dispensing, packaging, and storing of drugs and other preparations used in diagnostic and determinative procedures, and in the treatment of patients.
Hard or soft soluble containers used for the oral administration of medicine.
A series of progressive, overlapping events, triggered by exposure of the PLATELETS to subendothelial tissue. These events include shape change, adhesiveness, aggregation, and release reactions. When carried through to completion, these events lead to the formation of a stable hemostatic plug.
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.
Constituent composed of protein and phospholipid that is widely distributed in many tissues. It serves as a cofactor with factor VIIa to activate factor X in the extrinsic pathway of blood coagulation.
The process of the interaction of BLOOD COAGULATION FACTORS that results in an insoluble FIBRIN clot.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
Substances made up of an aggregation of small particles, as that obtained by grinding or trituration of a solid drug. In pharmacy it is a form in which substances are administered. (From Dorland, 28th ed)
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Platelet membrane glycoprotein IIb is an integrin alpha subunit that heterodimerizes with INTEGRIN BETA3 to form PLATELET GLYCOPROTEIN GPIIB-IIIA COMPLEX. It is synthesized as a single polypeptide chain which is then postranslationally cleaved and processed into two disulfide-linked subunits of approximately 18 and 110 kDa in size.
Polymerized forms of styrene used as a biocompatible material, especially in dentistry. They are thermoplastic and are used as insulators, for injection molding and casting, as sheets, plates, rods, rigid forms and beads.
A trace element that constitutes about 27.6% of the earth's crust in the form of SILICON DIOXIDE. It does not occur free in nature. Silicon has the atomic symbol Si, atomic number 14, and atomic weight [28.084; 28.086].
Term used to designate tetrahydroxy aldehydic acids obtained by oxidation of hexose sugars, i.e. glucuronic acid, galacturonic acid, etc. Historically, the name hexuronic acid was originally given to ascorbic acid.
Agents that cause clotting.
Anhydride polymers with a repeating structure of RC(=O)OC(=O)R. They readily hydrolyze in water making them useful for DELAYED-ACTION PREPARATIONS.
A sugar acid formed by the oxidation of the C-6 carbon of GLUCOSE. In addition to being a key intermediate metabolite of the uronic acid pathway, glucuronic acid also plays a role in the detoxification of certain drugs and toxins by conjugating with them to form GLUCURONIDES.
Characteristics or attributes of the outer boundaries of objects, including molecules.
Completed forms of the pharmaceutical preparation in which prescribed doses of medication are included. They are designed to resist action by gastric fluids, prevent vomiting and nausea, reduce or alleviate the undesirable taste and smells associated with oral administration, achieve a high concentration of drug at target site, or produce a delayed or long-acting drug effect.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours.
Poly-2-methylpropenoic acids. Used in the manufacture of methacrylate resins and plastics in the form of pellets and granules, as absorbent for biological materials and as filters; also as biological membranes and as hydrogens. Synonyms: methylacrylate polymer; poly(methylacrylate); acrylic acid methyl ester polymer.
Usually inert substances added to a prescription in order to provide suitable consistency to the dosage form. These include binders, matrix, base or diluent in pills, tablets, creams, salves, etc.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
Method of using a polycrystalline powder and Rietveld refinement (LEAST SQUARES ANALYSIS) of X-RAY DIFFRACTION or NEUTRON DIFFRACTION. It circumvents the difficulties of producing single large crystals.
Condition of having pores or open spaces. This often refers to bones, bone implants, or bone cements, but can refer to the porous state of any solid substance.
Differential thermal analysis in which the sample compartment of the apparatus is a differential calorimeter, allowing an exact measure of the heat of transition independent of the specific heat, thermal conductivity, and other variables of the sample.
Salts of alginic acid that are extracted from marine kelp and used to make dental impressions and as absorbent material for surgical dressings.
Platelet membrane glycoprotein complex essential for normal platelet adhesion and clot formation at sites of vascular injury. It is composed of three polypeptides, GPIb alpha, GPIb beta, and GPIX. Glycoprotein Ib functions as a receptor for von Willebrand factor and for thrombin. Congenital deficiency of the GPIb-IX complex results in Bernard-Soulier syndrome. The platelet glycoprotein GPV associates with GPIb-IX and is also absent in Bernard-Soulier syndrome.
Water swollen, rigid, 3-dimensional network of cross-linked, hydrophilic macromolecules, 20-95% water. They are used in paints, printing inks, foodstuffs, pharmaceuticals, and cosmetics. (Grant & Hackh's Chemical Dictionary, 5th ed)
Formation and development of a thrombus or blood clot in the blood vessel.
A complex mixture of PHOSPHOLIPIDS; GLYCOLIPIDS; and TRIGLYCERIDES; with substantial amounts of PHOSPHATIDYLCHOLINES; PHOSPHATIDYLETHANOLAMINES; and PHOSPHATIDYLINOSITOLS, which are sometimes loosely termed as 1,2-diacyl-3-phosphocholines. Lecithin is a component of the CELL MEMBRANE and commercially extracted from SOYBEANS and EGG YOLK. The emulsifying and surfactant properties are useful in FOOD ADDITIVES and for forming organogels (GELS).
The study of MAGNETIC PHENOMENA.
Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.
Colloids formed by the combination of two immiscible liquids such as oil and water. Lipid-in-water emulsions are usually liquid, like milk or lotion. Water-in-lipid emulsions tend to be creams. The formation of emulsions may be aided by amphiphatic molecules that surround one component of the system to form MICELLES.
Colloids with a gaseous dispersing phase and either liquid (fog) or solid (smoke) dispersed phase; used in fumigation or in inhalation therapy; may contain propellant agents.
Antibodies that are chemically bound to a substrate material which renders their location fixed.
A network of cross-linked hydrophilic macromolecules used in biomedical applications.
Removal of moisture from a substance (chemical, food, tissue, etc.).
The administration of drugs by the respiratory route. It includes insufflation into the respiratory tract.
An enzyme formed from PROTHROMBIN that converts FIBRINOGEN to FIBRIN.
A cell line established in 1962 from disaggregated Swiss albino mouse embryos. This fibroblast cell line is extremely popular in research.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.
Technique whereby the weight of a sample can be followed over a period of time while its temperature is being changed (usually increased at a constant rate).
Cell adhesion molecule and CD antigen that mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells.
Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.
Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.
A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.
Coating with a metal or alloy by electrolysis.
The design or construction of objects greatly reduced in scale.
The testing of materials and devices, especially those used for PROSTHESES AND IMPLANTS; SUTURES; TISSUE ADHESIVES; etc., for hardness, strength, durability, safety, efficacy, and biocompatibility.
Cell adhesion molecules present on virtually all monocytes, platelets, and granulocytes. CD31 is highly expressed on endothelial cells and concentrated at the junctions between them.
Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.
A cellulose derivative which is a beta-(1,4)-D-glucopyranose polymer. It is used as a bulk laxative and as an emulsifier and thickener in cosmetics and pharmaceuticals and as a stabilizer for reagents.
Suspensions of killed or attenuated microorganisms (bacteria, viruses, fungi, protozoa), antigenic proteins, synthetic constructs, or other bio-molecular derivatives, administered for the prevention, amelioration, or treatment of infectious and other diseases.
A chlorinated hydrocarbon that has been used as an inhalation anesthetic and acts as a narcotic in high concentrations. Its primary use is as a solvent in manufacturing and food technology.
The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.
A product formed from skin, white connective tissue, or bone COLLAGEN. It is used as a protein food adjuvant, plasma substitute, hemostatic, suspending agent in pharmaceutical preparations, and in the manufacturing of capsules and suppositories.
A nonimmunologic, chemically induced type of photosensitivity producing a sometimes vesiculating dermatitis. It results in hyperpigmentation and desquamation of the light-exposed areas of the skin.
High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.
The process by which blood or its components are kept viable outside of the organism from which they are derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
Derivatives of caproic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a carboxy terminated six carbon aliphatic structure.
Methylester of cellulose. Methylcellulose is used as an emulsifying and suspending agent in cosmetics, pharmaceutics and the chemical industry. It is used therapeutically as a bulk laxative.
Two-phase systems in which one is uniformly dispersed in another as particles small enough so they cannot be filtered or will not settle out. The dispersing or continuous phase or medium envelops the particles of the discontinuous phase. All three states of matter can form colloids among each other.
A type of extracellular vesicle, containing RNA and proteins, that is secreted into the extracellular space by EXOCYTOSIS when MULTIVESICULAR BODIES fuse with the PLASMA MEMBRANE.
Homogeneous liquid preparations that contain one or more chemical substances dissolved, i.e., molecularly dispersed, in a suitable solvent or mixture of mutually miscible solvents. For reasons of their ingredients, method of preparation, or use, they do not fall into another group of products.
The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
A property of the surface of an object that makes it stick to another surface.
Endogenous substances, usually proteins, that are involved in the blood coagulation process.
A CHROMATOGRAPHY method using supercritical fluid, usually carbon dioxide under very high pressure (around 73 atmospheres or 1070 psi at room temperature) as the mobile phase. Other solvents are sometimes added as modifiers. This is used both for analytical (SFC) and extraction (SFE) purposes.
Techniques where DNA is delivered directly into organelles at high speed using projectiles coated with nucleic acid, shot from a helium-powered gun (gene gun). One of these techniques involves immunization by DNA VACCINES, which delivers DNA-coated gold beads to the epidermis.
White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
The giving of drugs, chemicals, or other substances by mouth.
The residual portion of BLOOD that is left after removal of BLOOD CELLS by CENTRIFUGATION without prior BLOOD COAGULATION.
Vesicles that are involved in shuttling cargo from the interior of the cell to the cell surface, from the cell surface to the interior, across the cell or around the cell to various locations.
Transparent, tasteless crystals found in nature as agate, amethyst, chalcedony, cristobalite, flint, sand, QUARTZ, and tridymite. The compound is insoluble in water or acids except hydrofluoric acid.
A disorder of HEMOSTASIS in which there is a tendency for the occurrence of THROMBOSIS.
Manufacturing technology for making microscopic devices in the micrometer range (typically 1-100 micrometers), such as integrated circuits or MEMS. The process usually involves replication and parallel fabrication of hundreds or millions of identical structures using various thin film deposition techniques and carried out in environmentally-controlled clean rooms.
Agents causing contraction of the pupil of the eye. Some sources use the term miotics only for the parasympathomimetics but any drug used to induce miosis is included here.
Inorganic or organic compounds containing trivalent iron.
The quality or state of being wettable or the degree to which something can be wet. This is also the ability of any solid surface to be wetted when in contact with a liquid whose surface tension is reduced so that the liquid spreads over the surface of the solid.
Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
The extent to which the active ingredient of a drug dosage form becomes available at the site of drug action or in a biological medium believed to reflect accessibility to a site of action.
Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)
An anionic surface-active agent used for its wetting properties in industry and used in medicine as an irritant and sclerosing agent for hemorrhoids and varicose veins.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Apparatus, devices, or supplies intended for one-time or temporary use.
Tree-like, highly branched, polymeric compounds. They grow three-dimensionally by the addition of shells of branched molecules to a central core. The overall globular shape and presence of cavities gives potential as drug carriers and CONTRAST AGENTS.
Microdevices that combine microfluidics technology with electrical and/or mechanical functions for analyzing very small fluid volumes. They consist of microchannels etched into substrates made of silicon, glass, or polymer using processes similar to photolithography. The test fluids in the channels can then interact with different elements such as electrodes, photodetectors, chemical sensors, pumps, and valves.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
A polyhedral CARBON structure composed of around 60-80 carbon atoms in pentagon and hexagon configuration. They are named after Buckminster Fuller because of structural resemblance to geodesic domes. Fullerenes can be made in high temperature such as arc discharge in an inert atmosphere.
A diuretic and renal diagnostic aid related to sorbitol. It has little significant energy value as it is largely eliminated from the body before any metabolism can take place. It can be used to treat oliguria associated with kidney failure or other manifestations of inadequate renal function and has been used for determination of glomerular filtration rate. Mannitol is also commonly used as a research tool in cell biological studies, usually to control osmolarity.
The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
The analysis of a chemical substance by inserting a sample into a carrier stream of reagent using a sample injection valve that propels the sample downstream where mixing occurs in a coiled tube, then passes into a flow-through detector and a recorder or other data handling device.
Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Endothelial cells that line venous vessels of the UMBILICAL CORD.
A phthalic indicator dye that appears yellow-green in normal tear film and bright green in a more alkaline medium such as the aqueous humor.
The engulfment and degradation of cells by other cells.
The chemical and physical integrity of a pharmaceutical product.
Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)
Colloids with liquid continuous phase and solid dispersed phase; the term is used loosely also for solid-in-gas (AEROSOLS) and other colloidal systems; water-insoluble drugs may be given as suspensions.
Methods of creating machines and devices.
Derivatives of ACETIC ACID which contain an hydroxy group attached to the methyl carbon.
An involuntary contraction of a muscle or group of muscles. Spasms may involve SKELETAL MUSCLE or SMOOTH MUSCLE.
Nanometer-scale wires made of materials that conduct electricity. They can be coated with molecules such as antibodies that will bind to proteins and other substances.
Established cell cultures that have the potential to propagate indefinitely.
Tablets coated with material that delays release of the medication until after they leave the stomach. (Dorland, 28th ed)
A non-steroidal anti-inflammatory agent with analgesic, anti-inflammatory, and antipyretic properties. It is an inhibitor of cyclooxygenase.
Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
The clear, viscous fluid secreted by the SYNOVIAL MEMBRANE. It contains mucin, albumin, fat, and mineral salts and serves to lubricate joints.
The coagulation of tissue by an intense beam of light, including laser (LASER COAGULATION). In the eye it is used in the treatment of retinal detachments, retinal holes, aneurysms, hemorrhages, and malignant and benign neoplasms. (Dictionary of Visual Science, 3d ed)

Tissue factor activity is increased in a combined platelet and microparticle sample from cancer patients. (1/383)

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Microparticles are vectors of paradoxical information in vascular cells including the endothelium: role in health and diseases. (2/383)

Both inflammation and thrombosis can be orchestrated by the interactions between circulating cells, such as leukocytes and platelets, with vascular, endothelial and smooth muscle cells, which, during activation or apoptosis, can release circulating microparticles (MPs). Indeed, MPs are membrane vesicles with procoagulant and proinflammatory properties. MPs are present in blood from healthy individuals and in patients under several pathological states, for instance sepsis, preeclampsia, Crohn's disease and diabetes, strengthening the notion that MPs may play a role in these diseases. Circulating MPs or those generated in vitro from apoptotic T cells display deleterious effects on endothelial and/or vasomotor function. In contrast, MPs might be protective to endothelial cells. We have shown that MPs harboring the morphogen sonic hedgehog may represent a new therapeutic approach against endothelial dysfunction during acute severe endothelial injury. Indeed, these types of MPs induce NO release, decrease production of reactive oxygen species and induce angiogenesis from endothelial cells. This protective role for the endothelium was confirmed also by their in vivo injection in mice in which they were also able to reverse endothelial dysfunction in a model of heart ischemia/reperfusion. On the contrary, MPs from preeclamptic women compared to those from normal pregnant women showed pro-inflammatory properties in the vascular wall inducing vascular hyporeactivity in vessels from humans and mice. These effects were associated with complex interactions between NO and cyclooxygenase systems via endothelial cell activation. Altogether, these findings suggest that MPs can be considered as vectors of biological messages for vascular homeostasis, during immunity and inflammation.  (+info)

The role of cell death in the pathogenesis of autoimmune disease: HMGB1 and microparticles as intercellular mediators of inflammation. (3/383)

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The relationship between plasma microparticles and disease manifestations in patients with systemic sclerosis. (4/383)

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Measurement of platelet-derived microparticle levels in the chronic phase of cerebral infarction using an enzyme-linked immunosorbent assay. (5/383)

Assessment of platelet function is a critical component of the treatment and secondary prevention of cerebral infarction, and measurement of platelet-derived microparticle (PDMP) levels using flow cytometry may be a good indicator of platelet function. However, the flow cytometric analysis is not feasible in a variety of clinical situations. The goal of the present study was to measure PDMP levels using an enzyme-linked immunosorbent assay (ELISA) in chronic cerebral infarction patients and to determine the utility of PDMP level measurement for the monitoring of the effect of cilostazol and aspirin. A crossover study was performed using 4-weeks of aspirin (100 mg/day) and 4-weeks of cilostazol (200 mg/day) in 18 patients. PDMP levels were also measured in 20 volunteers as controls. Experiments demonstrated that PDMP levels were significantly higher in chronic cerebral infarction patients (median 8.8 U/ml, interquartile range 5.1-14.9 U/ml, n=18) than in controls (median 5.5 U/ml, interquartile range 5.0-8.2 U/ml, n=20) (P=0.047). PDMP levels did not decrease after therapy with either aspirin (median 10.9 U/ml, interquartile range 6.2-17.9 U/ml, n=12) or cilostazol (median 9.2 U/ml, interquartile range 6.1-14.3 U/ml, n=12) compared with baseline PDMP levels in the 12 patients who completed this trial (median 11.4 U/ml, interquartile range 5.2-23.7 U/ml, n=12). There were no significant differences in PDMP levels between aspirin and cilostazol (P=0.61). In conclusion, PDMP levels as measured by ELISA were increased in patients with chronic cerebral infarction regardless of the anti-platelet therapy. This methodology may be a useful strategy of assessing platelet function in chronic cerebral infarction patients.  (+info)

Megakaryocyte-derived microparticles: direct visualization and distinction from platelet-derived microparticles. (6/383)

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Dynamics of circulating microparticles in liver transplant patients. (7/383)

BACKGROUND & AIMS: Microparticles are small membrane vesicles released from the cell plasma membrane, particularly in cell stress, apoptosis and altered cellular viability. Hepatocellular carcinoma (HCC) is a hypervascular neoplasm with high levels of apoptosis and necrosis. We investigated the levels of circulating microparticles of both tumor and endothelial origins in liver transplant patients with hepatitis C (HepC) cirrhosis with and without HCC and compared them with healthy people and patients with partial hepatectomy. METHODS: Using immunolabeling of microparticles of different origin and flow cytometry-based enumeration of microparticles, the levels of circulating microparticles were studied in 8 patients with HepC and 8 patients with both HepC and HCC before and within two weeks after the transplant. RESULTS: The initial levels of circulating microparticles were increased in patients with HepC and HCC as compared to patients with HepC alone. They were also increased in liver transplant patients as compared to patients with partial hepatectomy or healthy people. Levels of circulating microparticles were dynamically changing after the transplant, showing an initial increase with a subsequent decrease by the end of the second week after surgery. In some patients with a complicated clinical outcome, the levels of microparticles were continuously increasing after the surgery. CONCLUSION: The levels of circulating microparticles of endothelial and hepatic origin in liver transplant patients dynamically change after surgery and correlate with the clinical outcome. Perspectively, the levels of circulating microparticles may be used in clinical practice as a marker of the functional status of the transplanted liver.  (+info)

Diagnostic role of endothelial microparticles in vasculitis. (8/383)

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After long controversy, it is now recognized that essentially all neurodegenerative diseases have inflammatory components [1]. Many other neurological disorders are exacerbated by inflammation, including the progressive impairment following stroke or traumatic brain injury (TBI). It has been known that levels of circulating cell-derived microparticles (MP) are generally increased in inflammatory states, making them useful as biomarkers. More recently, certain species of MP or exo- somes have been implicated as causative agents in neuroinflammation, to be reviewed.. L.L. Horstman, BS • W. Jy, PhD (*) • Y.S. Ahn, MD. University of Miami, Department of Medicine (Wallace H. Coulter Platelet Laboratory), 1600 NW 10TH Ave., Mail Code R36A, Miami, FL 33136, USA e-mail: This email address is being protected from spam bots, you need Javascript enabled to view it © Springer International Publishing AG 2017. A. Minagar, J.S. Alexander (eds.), Inflammatory Disorders of the Nervous System, Current ...
0175]1. Hugel B, Martinez M C, Kunzelmann C, Freyssinet J M. Membrane microparticles: two sides of the coin. Physiology (Bethesda, Md. February 2005; 20:22-27. [0176]2. Mallat Z, Benamer H, Hugel B, Benessiano J, Steg P G, Freyssinet J M, Tedgui A. Elevated levels of shed membrane microparticles with procoagulant potential in the peripheral circulating blood of patients with acute coronary syndromes. Circulation. Feb. 29 2000; 101(8):841-843. [0177]3. Nieuwland R, Berckmans R J, Rotteveel-Eijkman R C, Maquelin K N, Roozendaal K J, Jansen P G, ten Have K, Eijsman L, Hack C E, Sturk A. Cell-derived microparticles generated in patients during cardiopulmonary bypass are highly procoagulant. Circulation. Nov. 18 1997; 96(10):3534-3541. [0178]4. Tesse A, Martinez M C, Hugel B, Chalupsky K, Muller C D, Meziani F, Mitolo-Chieppa D, Freyssinet J M, Andriantsitohaina R. Upregulation of proinflammatory proteins through NF-kappaB pathway by shed membrane microparticles results in vascular hyporeactivity. ...
TY - JOUR. T1 - Phenotypic assessment of endothelial microparticles in patients with heart failure and after heart transplantation. T2 - Switch from cell activation to apoptosis. AU - Garcia, Santiago. AU - Chirinos, Julio. AU - Jimenez, Javier. AU - Del Carpio Muñoz, Freddy. AU - Canoniero, Mariana. AU - Jy, Wenche. AU - Jimenez, Joaquin J. AU - Horstman, Lawrence. AU - Ahn, Yeon. PY - 2005/12/1. Y1 - 2005/12/1. N2 - Background: Endothelial microparticles (EMPs) are sub-microscopic membrane vesicles that are shed from the surface of endothelial cells during activation, injury and/or apoptosis. Endothelial cells release phenotypically and quantitatively distinct endothelial microparticles (EMPs) in activation and apoptosis. Therefore, the phenotypic assessment of EMPs can provide useful information reflecting the nature of endothelial injury. We tested the hypothesis that heart transplantation (HT) modifies the pattern of endothelial injury seen in patients with congestive heart failure (CHF). ...
Acute HIV-1 infection results in dysregulated immunity, which contributes to poor control of viral infection. DCs are key regulators of both adaptive and innate immune responses needed for controlling HIV-1, and we surmised that factors elicited during acute HIV-1 infection might impede DC function. We derived immature DCs from healthy donor peripheral blood monocytes and treated them with plasma from uninfected control donors and donors with acute HIV-1 infections. We found that the plasma from patients with HIV specifically inhibited DC function. This suppression was mediated by elevated apoptotic microparticles derived from dying cells during acute HIV-1 infection. Apoptotic microparticles bound to and inhibited DCs through the hyaluronate receptor CD44. These data suggest that targeting this CD44-mediated inhibition by apoptotic microparticles could be a novel strategy to potentiate DC activation of HIV-specific immunity.
Recurrent miscarriage is frustrating for the physician and a heartbreaking experience for the patient. Approximately 5% of couples trying to conceive have two consecutive miscarriages. Despite a thorough study of patients, the aetiology of this common obstetric complication is unknown in 50% of cases. Known causes include abnormal chromosomes, endocrinological disorders and uterine abnormalities. Although antiphospholipid antibodies have been demonstrated in miscarriages, the role played by alloimmune mechanisms remains unclear. New immunological approaches such as natural killer cells, regulatory T cells, tumour necrosis factor α, cell-derived microparticles, leptin, certain glycoproteins and cytokines should be considered. The management of thyroid diseases and immunological disorders is continuously evolving. Several genetic diagnostic procedures such as parental karyotyping and preimplantation genetic screening should probably not be used routinely. Antiphopholipid syndrome and some ...
TY - JOUR. T1 - Circulating endothelial microparticles as a measure of early lung destruction in cigarette smokers. AU - Gordon, Cynthia. AU - Gudi, Kirana. AU - Krause, Anja. AU - Sackrowitz, Rachel. AU - Harvey, Ben Gary. AU - Strulovici-Barel, Yael. AU - Mezey, Jason G.. AU - Crystal, Ronald. PY - 2011/7/15. Y1 - 2011/7/15. N2 - Rationale: There is increasing evidence thatemphysemais associated with primary loss of pulmonary capillary endothelium. Plasma levels of endothelial microparticles (EMPs), small vesicles released from activated or apoptotic endothelial cells, are elevated in vascularrelated disorders. Objectives: To evaluate whether plasma EMP levels are elevated in smokers with early lung destruction as assessed by normal spirometry but reduced diffusing capacity of the lung for carbon monoxide (DLCO). Methods: Lung health was assessed by pulmonary function tests (PFTs: spirometry, total lung capacity, DLCO) and chest X-ray; smoking status was assessed by urine nicotine and ...
TY - JOUR. T1 - C-reactive protein induces release of both endothelial microparticles and circulating endothelial cells in vitro and in vivo. T2 - Further evidence of endothelial dysfunction. AU - Devaraj, Sridevi. AU - Kumaresan, Pappanaicken R.. AU - Jialal, Ishwarlal. PY - 2011/12. Y1 - 2011/12. N2 - BACKGROUND: Inflammation is pivotal in atherosclerosis. A key early event in atherosclerosis is endothelial dysfunction. C-reactive protein (CRP), the prototypic marker of inflammation in humans, is a risk marker for cardiovascular disease, and there is mounting evidence to support its role in atherothrombosis. CRP has been shown to promote endothelial dysfunction both in vitro and in vivo. Emerging biomarkers of endothelial dysfunction include circulating endothelial cells (CECs) and endothelial microparticles (EMPs). However, there is a paucity of data examining the effect of CRP on CEC and EMP production in vitro and in vivo. METHODS: In this report, we treated human aortic endothelial cells ...
The results of these studies demonstrate that calcineurin inhibitors cause endothelial cells to increase the number of microparticles released into the circulation from the cell surface. Calcineurin inhibitors also alter the composition of the microparticles such that they become complement activating. We found that alternative pathway-deficient mice are protected from CsA-induced renal and vascular injury. We also found that endothelial microparticles cause injury of unmanipulated endothelial cells in vitro, and they cause mesangial proliferation and complement activation when passively transferred into wild-type mice in vivo. These results demonstrate that CsA-induced endothelial microparticles can cause bystander injury of endothelial cells, and they promote glomerular complement activation and mesangial expansion. Furthermore, preliminary data from human transplant patients indicate that treatment of these patients with tacrolimus is also associated with generation of endothelial ...
Our study demonstrates that CRC cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells, suggesting that microvesicles of cancer cells can be involved in tumor growth and metastasis by facilitating angiogenesis-related processes. This infor …
Maturation (MP) or polishing ponds are widely used throughout the world as a final or tertiary wastewater treatment process for effluent quality improvement from secondary biological treatment systems, including FPs. MPs can be expected to reduce total suspended solids (TSS), ammonia, nitrate and phosphorus levels in effluent, but the primary function of MPs in most parts of the world is the removal of pathogenic organisms. A number of mechanisms in MPs are responsible for pathogen reduction, including settlement, natural die off (a function of time and temperature), elevated pH, UV radiation and predation. The design, size and number (usually in series) of MPs are typically based on the desired bacteriological quality of the final effluent. MPs are typically designed at the same depth as facultative ponds (2 to 5 feet (0.7 to 1.5 m)). Retention times in MPs are typically designed at 10 to 14 days for pathogen reduction; however, shorter retention times are required for TSS removal (four to five ...
Microparticles are membrane vesicles with procoagulant and proinflammatory properties released during cell activation or apoptosis. Microparticles from monocytes have been implicated in atherosclerosis and vascular inflammation, but their direct effects on endothelial cells are not completely elucidated. The present study was designed to dissect the signaling pathways of monocytic microparticles in endothelial cells with respect to both NO pathway and reactive oxygen species. Microparticles were produced by treatment of human monocytic cell line THP-1 with the apoptotic agent VP-16. Human endothelial cells were treated with monocytic microparticles and then, we studied their effects on nitrosative and oxidative stresses. Incubation of human endothelial cells with microparticles enhanced the production of NO without affecting superoxide anions generation. Microparticles did not affect endothelial NO synthase expression and its phosphorylation. Interestingly, microparticles decreased caveolin-1 ...
Microparticles are a heterogeneous population of membrane-coated vesicles which can be released from virtually all cell types during activation or apoptosis. Release occurs from the cell surface in an exogenous budding process involving local rearrangement of the cytoskeleton. Given their origin, these particles can be identified by staining for cell surface markers and annexin V. As shown in in vitro studies, microparticles may represent a novel subcellular element for intercellular communication in inflammation. Thus, microparticles can transfer chemokine receptors and arachidonic acid between cells, activate complement, promote leukocyte rolling and stimulate the release of pro-inflammatory mediators. Under certain conditions, however, microparticles may also exert anti-inflammatory properties by inducing immune cell apoptosis and the production of anti-inflammatory mediators. Microparticles may play an important role in the pathogenesis of rheumatologic diseases as evidenced by their elevation in
Simoncini S, Njock MS, Robert S, Camoin-Jau L, Sampol J, Harlé JR, Nguyen C, Dignat-George F, Anfosso F. Circ Res. 2009 Apr 24 ;104 (8):943-51 (...)
Oxidative Medicine and Cellular Longevity is a unique peer-reviewed, Open Access journal that publishes original research and review articles dealing with the cellular and molecular mechanisms of oxidative stress in the nervous system and related organ systems in relation to aging, immune function, vascular biology, metabolism, cellular survival and cellular longevity. Oxidative stress impacts almost all acute and chronic progressive disorders and on a cellular basis is intimately linked to aging, cardiovascular disease, cancer, immune function, metabolism and neurodegeneration. The journal fills a significant void in todays scientific literature and serves as an international forum for the scientific community worldwide to translate pioneering
Microparticles (MPs) are fragments of cell membranes released from stimulated or apoptotic cells that have long been considered innocent remnants of cell destruction. Evidence is accumulating, however, that the circulating and tissue-derived MPs may elicit a strong thrombogenic and inflammatory response (1). In addition to MPs, activated cells can also release smaller vesicle-denominated exosomes that are relatively unexplored in the research area of vascular biology (2).. Circulating MPs are being explored as a potential source for biomarker discovery. Platelet MPs have been associated with clinically evident atherosclerotic disease in diabetes type II patients (3), and leukocyte-derived MPs are predictive of subclinical atherosclerosis in asymptomatic patients (4).. Experimental and clinical data consistently point to a causal role for MPs in atherosclerosis development and progression. Recent reports revealed their contribution to hemostatic and inflammatory responses, vascular remodeling and ...
Background: Early and non-invasive biomarkers of kidney damage are needed to identify hypertensive patients at risk for kidney damage. Urinary micropaticles (UMPs) have gained significant attention as potential novel biomarkers for kidney damage, and have already been identified in pre-albuminuric diabetic glomerular injury. These vesicles are less than 1 micron in size and carry markers of the parent cell. We hypothesized that podocyte derived UMPs are elevated in angiotensin II-induced hypertension (HTN). Methods: Primary podocytes were isolated and grown in culture. Wild-type mice were treated with AII (400ng/kg/min) via mini-osmotic pumps. Untreated WT mice served as controls. 24 hour urines were collected after 5 days of AII treatment. Enumeration and phenotyping of MPs was done of podocyte culture supernatant and urine. Podocalyxin (Pcal), podoplanin (Ppla) and annexin 5 (AV) were used as surface markers.. Result: Pcal and Ppla positive MPs as well as AV positive and negative MPs were ...
CA153 : The instrument used is the Roche Cobas. The Roche cancer antigen 15-3 (CA 15-3) method is a sandwich electrochemiluminescence immunoassay that employs a biotinylated monoclonal CA 15-3-specific antibody and a monoclonal CA 15-3-specific antibody. CA 15-3 in the automatically prediluted specimen reacts with both the biotinylated monoclonal CA 15-3-specific antibody (mouse) and the monoclonal CA 15-3-specific antibody (mouse) labeled with a ruthenium complex, forming a sandwich complex. Streptavidin-coated microparticles are added and the mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell. Application of voltage to the electrode induces the chemiluminescent emission, which is then measured.(Package insert: Roche CA 15-3 reagent, Roche Diagnostic Corp., Indianapolis, IN 2010-09, V14)
BOSTON, Massachusetts (PNN) - March 28, 2017 - A team of scientists at the Boston Childrens Hospital have invented what is being considered one the greatest medical breakthroughs in recent years. They have designed a microparticle that can be injected into a persons bloodstream that can quickly oxygenate his or her blood. This will even work if the ability to breathe has been restricted, or even cut off entirely.. This finding has the potential to save millions of lives every year. The microparticles can keep an object alive for up to 30 minutes after respiratory failure. This is accomplished through an injection into the patients veins. Once injected, the microparticles can oxygenate the blood to near normal levels. This has countless potential uses as it allows life to continue when oxygen is needed but unavailable. For medical personnel, this is just enough time to avoid risking a heart attack or permanent brain injury when oxygen is restricted or cut off to patient.. Dr. John Kheir, who ...
2015. Ghelani H, Razmovski-Naumovski V, Kyada AV, Parekh HS, Li GQ, Nammi S. Myoglobin and low density lipoprotein glycation in cardiac complications of diabetes: Current perspectives and therapeutic options. Indian J Med Res. 2015. In Press. Jadeja R, Devkar RV, Nammi S. Hepatoprotective potential of herbal medicine. Evid-Based Compl Alt. [Editorial]. 2015;2015.. Kam A, Li KM, Razmovski-Naumovski V, Nammi S, Chan K, Grau GE, et al. Curcumin reduces tumour necrosis factor-enhanced annexin V-positive microparticle release in human vascular endothelial cells. J Pharm Pharm Sci. 2015;18(4):424-33.. Thounaojam MC, Nammi S, Jadeja R. Natural products for the treatment of obesity, metabolic syndrome, and type 2 diabetes 2014. Evid-Based Compl Alt. [Editorial]. 2015;2015. DOI: 10.1155/2015/392681.. Yang WC, Chang CLT, Li CR, Nammi S, Cho WCS. Complementary and alternative medicine for diabetes 2014. Evid-Based Compl Alt. [Editorial]. 2015;2015. DOI: 10.1155/2015/685248.. 2014. Li Y, Tran VH, Kota BP, ...
TY - JOUR. T1 - Modification of molecular conformation of spray-dried whey protein microparticles improving digestibility and release characteristics. AU - Ye, Qianyu. AU - Woo, Meng Wai. AU - Selomulya, Cordelia. PY - 2019/5/15. Y1 - 2019/5/15. N2 - This study reports on the preparation of riboflavin-loaded whey protein isolate (WPI) microparticles, using desolvation and then spray drying. Ethanol desolvation led to the exposure of embedded hydrophobic amino acids of WPI to riboflavin, facilitating the formation of riboflavin-WPI complexes. The extent of desolvation and cross-linking influenced the morphology of the spray-dried microparticles, while the moisture content of microparticles decreased with desolvation and increased with crosslinking. The modification of WPI conformation upon desolvation could be retained in the dry state via spray drying. The gastric resistance, release site and release characteristics of microparticles were readily adjusted by varying the ethanol and calcium ion ...
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Overall research in my lab explores the molecular mechanisms of cellular signal transduction, with several related foci: 1) intercellular signaling in platelet-tumor interactions, including the contributions of Ras family small G proteins; 2) the contributions of protein trafficking to the signaling and oncogenic functions of Ras proteins; 3) Ras signaling in platelets, regulating thrombosis and hemostasis. My long term goal is to reach a new understanding of cellular information processing and its contributions to signal responses and to translate these findings into pre-clinical approaches.. 1. Intercellular signaling in platelet-tumor interactions.. We recently discovered that platelet microparticles - small extracellular vesicles released by blood platelets into plasma - infiltrate solid tumors by means of the unusual permeability of tumor vasculature, whereupon the platelet microparticles anchor to tumor cells and transfer genetic information in the form of platelet-derived microRNAs ...
55% carbohydrates), with endothelial function measurement repeated after 1 year. As secondary objectives and to explore different underlying mechanisms in the modulation of endothelial function, we quantified endothelial microparticles (EMPs) and endothelial progenitor cells (EPCs) and evaluated, in 24 preselected patients, in vitro cellular processes related to endothelial damage (reactive oxygen species, apoptosis, and senescence) and endothelial repair (cell proliferation and angiogenesis), as well as other modulators (micro-RNAs [miRNAs] and proteins). Patients who followed the Mediterranean diet had higher FMD (3.83%; 95% confidence interval [CI]: 2.91-4.23) compared with those in the low-fat diet (1.16%; 95% CI: 0.80 to 1.98) with a difference between diets of 2.63% (95% CI: 1.89-3.40, p = 0.011), even in those patients with severe endothelial dysfunction. We observed higher EPC levels (group difference: 1.64%; 95% CI: 0.79-2.13, p = 0.028) and lower EMPs (group difference: -755 EMPs/µl; ...
VEGF/VEGFR inhibitors, used as anti-angiogenic drugs to treat cancer, induce severe hypertension. Molecular mechanisms are unclear, but nitric oxide (NO) and oxidative stress may be involved. We questioned whether reactive oxygen species (ROS) and Ang II also play a role in VEGF inhibitor-induced vascular dysfunction. Human microvascular endothelial cells (HMECs) were stimulated with vatalanib (VAT-VEGFR inhibitor) and gefitinib (GEF-EGFR inhibitor) in the absence/presence of Ang II. Activation of eNOS and MAPKs were assessed by immunoblotting. Antioxidant enzyme mRNA was analysed by qPCR. Endothelial microparticles, biomarkers of endothelial damage, tend to increase in subjects treated with VEGFR inhibitors. Phosphorylation of eNOS (28.3% ± 7.1) was decreased by VAT (p , 0.05). VAT decreased mRNA expression of Nox4 (0.5 ± 0.2) and H2O2-regulating antioxidants enzymes such as catalase (0.4 ± 0.1) and glutathione peroxidase (0.4 ± 0.1), while increased mRNA levels of Nox5 (3.35 ± 1.1) (p , ...
Background: Neopetrosiamide A (NeoA) is a 28-amino acid tricyclic peptide originally isolated from a marine sponge as a tumor cell invasion inhibitor whose mechanism of action is unknown. Methodology/Principal Findings: We show that NeoA reversibly inhibits tumor cell adhesion, disassembles focal adhesions in pre-attached cells, and decreases the level of beta 1 integrin subunits on the cell surface. NeoA also induces the formation of dynamic, membrane-bound protrusions on the surface of treated cells and the release of membrane-bound vesicles into the culture medium. Proteomic analysis indicates that the vesicles contain EGF and transferrin receptors as well as a number of proteins involved in adhesion and migration including: beta 1 integrin and numerous alpha integrin subunits; actin and actin-binding proteins such as cofilin, moesin and myosin 1C; and membrane modulating eps15 homology domain (EHD) proteins. Surface labeling, trafficking inhibition, and real-time imaging experiments all ...
M. ABBAS, L. JESEL, C. AUGER, L. AMOURA, N. MESSAS, G. MANIN, C. RUMIG, A.J. LEON-GONZALEZ, T.P. RIBEIRO, G.C. SILVA, R. ABOU-MERHI, E. HAMADE, M. HECKER, Y. GEORG, N. CHAKFE, P. OHLMANN, V.B. SCHINI-KERTH, F. TOTI & O. MOREL. Endothelial microparticles from acute coronary syndrome patients induce premature coronary artery endothelial cells ageing and thrombogenicity: role of the Ang II/AT1 receptor/NADPH oxidase-mediated activation of MAPKs and PI3-kinase pathways. Circulation 135, 280-296, 2017 - IF 17.20 - ...
Pitha J, Dorazilova Z, Melenovsky V, Kralova Lesna I, Stavek P, Stepankova J, Urban M, Maly J, Netuka I. The impact of left ventricle assist device on circulating endothelial microparticles - pilot study. Neuro Endocrinol Lett. 2012 Jan; 33(Suppl 2): 68-72 ...
A beam of accelerated microparticles of substantially uniform velocity is passed transversely through an intense pulsed laser beam. In traversing the laser beam, the microparticles are thermally evapo
TGF-β3 is enzymatically immobilized by transglutaminase-2 action to poly(L-lactic acid) microparticles coated with collagen II. Microparticles are then encapsulated with stem cells inside liquified spherical compartments enfolded with a permselective shell through layer-by-layer adsorption. Magnetic nanoparticles are electrostatically bound to the multilayered shell, conferring magnetic-response ability. The goal of this study is to engineer a closed environment inside which encapsulated stem cells would undergo a self-regulated chondrogenesis. To test this hypothesis, capsules are cultured in chondrogenic differentiation medium without TGF-β3. Their biological outcome is compared with capsules encapsulating microparticles without TGF-β3 immobilization and cultured in normal chondrogenic differentiation medium containing soluble TGF-β3. Glycosaminoglycans quantification demosntrates that similar chondrogenesis levels are achieved. Moreover, collagen fibrils resembling the native ...
Summary of Facts and Submissions. I. This appeal is from the interlocutory decision of the Opposition Division concerning maintenance of European patent No. 1 328 683 in amended form on the basis of the then pending main request. The independent Claim 1 reads:. 1. A process for making paper or paper board comprising forming a cellulosic suspension, flocculating the suspension, draining the suspension on a screen to form a sheet and then drying the sheet,. characterised in that. the suspension is flocculated using a flocculating system comprising a siliceous material and organic microparticles which have an unswollen particle diameter of less than 750 nanometers,. wherein a further flocculating material is included into the cellulosic suspension before adding the polymeric microparticles and siliceous material,. and in which the flocculating material is cationic and is a natural or synthetic polymer,. in which the microparticles are made from anionic copolymers comprising from 0 to 99 parts, by ...
The disassembly of apoptotic cells into small membrane-bound vesicles termed apoptotic bodies (ApoBDs) is a hallmark of apoptosis; however, the functi
A novel microparticle preparation comprising microparticles of a polymer which contain a drug and are coated with a film of an agent for preventing aggregation of the microparticles is disclosed. The preparation is produced by spraying a solution of a polymer containing a drug and an aqueous solution of an agent for preventing aggregation of the microparticles separately from different nozzles and contacting them with each other in a spray dryer.
The argument that we must do everything a Minister demands because he has been democratically chosen does not stand up to close inspection. MPs are not chosen by the people - they are chosen by their local constituency parties: thirty-five men in grubby raincoats or thirty-five women in silly hats. The further selection process is equally a nonsense: there are only 630 MPs and a party with just over 300 MPs forms a government and of these 300, 100 are too old and too silly to be ministers and 100 too young and too callow. Therefore there are about 100 MPs to fill 100 government posts. Effectively no choice at all ...
Macrophages (MPs) are heterogeneous, multifunctional, myeloid-derived leukocytes that are part of the innate immune system, playing wide-ranging critical roles in basic biological activities, including maintenance of tissue homeostasis involving clearance of microbial pathogens. Tumor-associated MPs …
Chepkwony said that they wanted the structure of the National Parliament replicated in county assemblies so that if MPs are appointed as Ministers then MCAs are also appointed as CECs.. In the coming months we shall hold an AGM for all MCAs and we shall combine our recommendations and hand them to the BBI taskforce, he said.. On his part, the forum chairman Ndegwa Wahome said that they were against the proposal for regional governments noting that Kenyans were currently over-represented.. Wahome who is also the Nyandarua Assembly Speaker, said that the current system should be strengthened adding that wards should be funded as one way of addressing the challenges facing Wanjiku.. ...
Thirty years for The Three Profiteers and the Parasite Peer : John Wright a writer and specialist in crime is outraged along with millions of other people that only four MPs are to be charged out of the hundreds who fiddled their expenses and stole public money John reckons we should give them all thirty y
Hospital parking charges have been slammed for placing an unfair financial burden on carers. MPs are looking to change the law to force health bosses to ex...
This petition has received 10,759 signatures. our MPS are dragging there feet on this . i have a letter from boris johnston who wants to bring in new regulations o...
A doctor who received a mystery package for Sir Bradley Wiggins has no record of his medical treatment at the time, MPs are told.
POSITEK 4G Microparticle Technology is the latest generation in Nalco microparticle innovation. This new programme is designed to promote drainage and retention without causing any negative effects caused by over-flocculation. | Ecolab
Vesicles are small membrane-bound structures containing materials for export. They combine with the outer cell membrane, releasing their contents. Epithelial cells form lining layers. ...
hi guys, Im in need of a lot of help. Basically my teacher is threatening to move me down to national 5, if I dont get one of the topic marks and prove myself I should stay at higher. But because my teacher cant teach and its impossible to find any notes Ill need to pass the prelim, Im screwed like hell. So if anyone has any good revision material there willing to share to me or useful tips I can better my no knowledge of rmps to knowing all that I need, that would be very nice of you. so can anyone help a brother from another, pleaseeee ...
Microparticles have been found to have widespread applications in medicine, biochemistry, colloid chemistry, and aerosol research.
View Notes - fulltext37 from SPECTOGRAP 545 at AIB College of Business. Nano- and Microparticles of Organic Fluorescent Dyes Self-organization and Optical Properties SUZANNE FERY-FORGUES, MOUHAMMAD
ananyo writes Rabbits with blocked windpipes have been kept alive for up to 15 minutes without a single breath, after researchers injected oxygen-filled microparticles into the animals blood. Oxygenating the blood by bypassing the lungs in this way could save the lives of people with impaired brea...
phagosome: A membrane-bound vesicle found in a cell by an inward folding of the cell membrane to hold foreign matter taken into the cell by phagocytosis.
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
An Indian-origin researcher has created tiny polymer beads that can slowly release anti-inflammatory drugs, and break down into non-toxic components.
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies. ...
It has been thirty years since one of the authors (EJD) began a collaboration with Professor Milton Kerker at Clarkson University in Potsdam, New
Microparticles are derived from many other cell types. Microvesicles and exosomes are formed and released by two slightly ... Microparticles derived from monocytes aggravate atherosclerosis by modulating inflammatory cells. Additionally, microvesicles ... For example, those released from antigen-presenting cells (APCs), such as B cells and dendritic cells, are enriched in proteins ... Once released from their cell of origin, microvesicles interact specifically with cells they recognize by binding to cell-type ...
"Macrophages discriminate glycosylation patterns of apoptotic cell-derived microparticles". The Journal of Biological Chemistry ... Although most cells are capable of phagocytosis, some cell types perform it as part of their main function. These are called ' ... One of the features of an apoptotic cell is the presentation of a variety of intracellular molecules on the cell surface, such ... Phagocytosis (from Ancient Greek φαγεῖν (phagein) 'to eat', and κύτος, (kytos) 'cell') is the process by which a cell uses its ...
It is derived from the bacteria that causes acne and targets myeloid cells through TLR9 and NOD2. In one of its first rounds of ... MIS416 is a microparticle derived from the cytoskeleton of P. acnes, a species of bacteria present on the skin of most adults ... Bacteria-derived microparticles have several advantages over traditional adjuvants related both to their size and biological ... a non-toxic microparticle adjuvant derived from Propionibacterium acnes comprising immunostimulatory muramyl dipeptide and ...
Kumacheva has made strong contributions in the area of self-assembly of nanoparticles and microparticles. Her research ... affect necrotic heart tissue as well as deter the metastasis of cancer cells. ... interests include nature-derived nanopartocles, e.g., cellulose nanocrystals and nanofibers ). Eugenia Kumacheva's awards and ... Kumacheva's work is the creation of hydrogels and various other chemical environments to either support the life of a stem cell ...
MIS416, a non-toxic microparticle adjuvant derived from Propionibacterium acnes comprising immunostimulatory muramyl dipeptide ... and Th17 cells in EAE, and [increased] the serum levels of IFN-γ and IFN-γ-associated proteins in 19 patients with SPMS." " ... "a myeloid-directed microparticle immune response modifier (derived from Cutibacterium acnes), which was originally developed as ... a non-toxic microparticle adjuvant derived from Propionibacterium acnes comprising immunostimulatory muramyl dipeptide and ...
"Detection of fetal DNA and RNA in placenta-derived syncytiotrophoblast microparticles generated in vitro". Clinical Chemistry. ... Cell-free fetal DNA (cffDNA) is fetal DNA that circulates freely in the maternal blood. Maternal blood is sampled by ... Approximately 11 to 13.4 percent of the cell-free DNA in maternal blood is of fetal origin. The amount varies widely from one ... Smaller fragments can represent up to seventy percent of the total cell free DNA in the maternal blood sample. In real-time PCR ...
... the HIV co-receptors CCR5 and CXCR4 could be transferred from an HIV-susceptible cell to a refractory cell by "microparticles ... A clinical trial of dendritic cell-derived EVs was performed in France just before the turn of the century.[citation needed] ... Since apoptotic cells tend to display phosphatidylserine (PS) in the outer bilayer of the cell membrane, apoptotic bodies tend ... They carry a cargo of proteins, nucleic acids, lipids, metabolites, and even organelles from the parent cell. Most cells that ...
Caveolae Cellular dewetting Endothelial activation Endothelial microparticle Endothelial progenitor cell Endothelium-derived ... Endothelial cells in direct contact with blood are called vascular endothelial cells whereas those in direct contact with lymph ... Endothelial cells actively signal to white blood cells of the immune system during inflammation Formation of new blood vessels ... Vascular endothelial cells line the entire circulatory system, from the heart to the smallest capillaries. These cells have ...
Martínez, MC; Tesse, A; Zobairi, F; Andriantsitohaina, R. Shed membrane microparticles from circulating and vascular cells in ... monocyte-derived and tissue factor-carrying circulating microparticles are related to acute myocardial infarction severity.. ... 胞漿顆粒(英语:Granule (cell biology)). 黑色素體 · 微粒體 · 過氧化物酶體 · 乙醛酸循環體 · 韋伯潘力氏小體(英语:Weibel-Palade body) ... A whole-cell electron tomography model of vacuole biogenesis in Arabidopsis root cells.. Nature plants
"Calculation of Single Cell Assimilation Rates From SIP-NanoSIMS-Derived Isotope Ratios: A Comprehensive Approach". Frontiers in ... NanoSIMS can also be used to examine the elemental and isotopic composition of microparticles preserved in the rock record. The ... The mouse gut microbiome was investigated to determine which microbes fed on host-derived compounds. For this, mice were given ... Peteranderl, R.; Lechene, C. (2004-04-01). "Measure of carbon and nitrogen stable isotope ratios in cultured cells". Journal of ...
In many plants, the laticiferous system is formed from rows of cells laid down in the meristem of the stem or root. The cell ... Since the 17th century, latex has been used as a term for the fluid substance in plants, deriving from the Latin word for " ... Latex is a stable dispersion (emulsion) of polymer microparticles in water. Latexes are found in nature, but synthetic latexes ... In the mature plant, the entire laticiferous system is descended from a single cell or group of cells present in the embryo. ...
Agarose is a polysaccharide derived from seaweed used for nanoencapsulation of cells and the cell/agarose suspension can be ... Alginate microparticles loaded with myoblast cells and functionalized with RGD allowed control over the growth and ... autologous cells), from another donor (allogeneic cells) or from other species (xenogeneic cells). The use of autologous cells ... The cell type chosen for this technique depends on the desired application of the cell microcapsules. The cells put into the ...
Moving into the cell membrane is the transmembrane domain, which is followed by the spacer domain located outside of the cell, ... "Macrophage scavenger receptors and host-derived ligands". Methods. 43 (3): 207-17. doi:10.1016/j.ymeth.2007.06.004. PMID ... "Therapeutic inflammatory monocyte modulation using immune-modifying microparticles". Science Translational Medicine. 6 (219): ... When ligand binds to MARCO on dendritic cells, the cytoskeleton of the cell is altered, allowing for the formation of the long ...
... dioxide Cotton Charcoal Smoke particulates Air pollutants Cosmetics-derived microparticles Drug nanoparticles Implant-derived ... Cells undergo cell death via three main mechanisms: nectoptosis via RIPK1, FADD, RIPK3, and MLKL, ferroptosis via GPX4 ... Necroinflammation is an autoamplifying process where crystals are toxic to cells (cytotoxicity) and cause cell death (necrosis ... form foam cells and giant cells, and try to do extracellular digestion in a process called frustrated phagocytosis. Crystals do ...
When used as scaffolds, hydrogels may contain human cells to repair tissue. They mimic 3D microenvironment of cells. Hydrogel- ... Other additives, such as nanoparticles and microparticles, have been shown to significantly modify the stiffness and gelation ... "The use of chitosan based hydrogel for enhancing the therapeutic benefits of adipose-derived MSCs for acute kidney injury". ... coated wells have been used for cell culture. Investigating biomechanical functions in cells when combined with Holotomography ...
"3.3 A cryo-EM structure of a nonenveloped virus reveals a priming mechanism for cell entry". Cell. 141 (3): 472-82. doi:10.1016 ... and micro-particles gave rise to a detailed publication, Ref. Degueldre et al (2006).[] Since 2010 the interest for SP ICP-MS ... "Dynamics in cryo EM reconstructions visualized with maximum-likelihood derived variance maps". Journal of Structural Biology. ... Å resolution CryoTEM structure of β-galactosidase in complex with a cell-permeant inhibitor". Science. 348 (6239): 1147-1151. ...
"Encapsulating bacteria in agarose microparticles using microfluidics for high-throughput cell analysis and isolation". ACS ... More recent advancements in microfluidic particles allowed the synthesis of nanometer sized particles from biologically derived ... Hydrocarbon oil is typically not used in cell microfluidic research because it is not compatible with cells and damages cell ... Droplets containing cells can be stored off-chip in PTFE tubing for up to several days while maintaining cell viability and ...
... but some compounds from the cell wall are unique and are used to trace bacterial derived DOC (e.g., peptidoglycan). These ... Self-organization of dissolved organic matter tomicelle-like microparticles in river water. Nature 422, 150-154. doi: 10.1038/ ... Bacteria are often viewed as the main consumers of DOC, but they can also produce DOC during cell division and viral lysis. The ... The lytic cycle causes disruption of the cell(s) and release of DOC. Marine macrophytes (i.e., macroalgae and seagrass) are ...
A theoretical expression for this spectrum is derived in the following, which can then be fitted to the experimental curve in ... Other applications are the rheology of soft matter, and studies of force-regulated processes in living cells. Forces are ... A magnetic tweezers apparatus consists of magnetic micro-particles, which can be manipulated with the help of an external ... In experiments, the molecule of interest is attached to a magnetic microparticle. The magnetic tweezer is equipped with magnets ...
These cells, together with other immune cells such as macrophages, lymphocytes, neutrophils, mast cells, dendritic cells and ... The word "synovium" is derived from the word "synovia" (or synovial fluid), which is a clear, viscous fluid produced by the ... 2010). "Platelets Amplify Inflammation in Arthritis via Collagen-Dependent Microparticle Production". Science. 327 (5965): 580- ... These hallmark features of FLS in RA are divided into 7 cell-intrinsic hallmarks and 4 cell-extrinsic hallmarks. The cell- ...
Cancer cells may facilitate export of PAI-2 via microparticles.[19] PAI-2 provides protection for cancer cells against plasmin- ... "Cloning and expression of a cDNA coding for a human monocyte-derived plasminogen activator inhibitor". Proceedings of the ... Among immune cells, macrophages are the main producers of PAI-2, as both B-cells and T-cells do not produce significant amounts ... "Cell. 156 (5): 1002-16. doi:10.1016/j.cell.2014.01.040. PMC 3988473. PMID 24581498.. ...
... has shown to induce apoptosis and promote antitumor actions on various cancer cell lines in vitro. Hinokinin has been ... Hinokinin is a dibenzylbutyrolactone lignan, derived from various species of plants. It is a potential antichagonistic agent ... microparticles for Chagas disease". Parasitology Research. 106 (3): 703-708. doi:10.1007/s00436-010-1725-1. ISSN 0932-0113. ...
A thick SEI layer results in a higher cell resistance, which decreases cell efficiency. The SEI layer on silicon is composed of ... These microparticles reached an energy density of 3,300 mAh/g. In 2015, Tesla founder Elon Musk claimed that silicon in Model S ... Polymer derived SiOC integrated with graphene aerogel as highly stable Li-ion battery anodes ACS Appl. Mater. Interfaces 2020, ... Lithium-silicon batteries also include cell configurations where Si is in compounds that may at low voltage store lithium by a ...
... antimetastatic activity of dendritic cells based on vaccines when mechanically heterogenized microparticles of tumor cells are ... One such model, the patient derived tumour xenograft, has shown excellent utility in preserving tumour heterogeneity whilst ... The cancer stem cell model asserts that within a population of tumour cells, there is only a small subset of cells that are ... There are two models used to explain the heterogeneity of tumour cells. These are the cancer stem cell model and the clonal ...
Cross-priming occurs when a bone marrow-derived cell presents peptides from proteins synthesised in another cell in the context ... that has been absorbed onto gold or tungsten microparticles into the target cells, using compressed helium as an accelerant. ... Studies using chimeric mice have shown that antigen is presented by bone-marrow derived cells, which include dendritic cells, ... The type of T-cell help raised is determined by the differentiated state of antigen presenting cells. Dendritic cells can ...
Contaminants inside the cells can defeat these safety devices. Also, these features can not be applied to all kinds of cells, e ... Polymer-Derived SiOC Integrated with a Graphene Aerogel As a Highly Stable Li-Ion Battery Anode ACS Appl. Mater. Interfaces ... Qi, Zhaoxiang; Koenig, Gary M. (16 August 2016). "High-Performance LiCoO2Sub-Micrometer Materials from Scalable Microparticle ... The 21700 cell has 50% more energy than the 18650 cell, and the bigger size reduces heat transfer to its surroundings. The ...
Using light microscopy, he observed macrophage cells laden with micro-particles in lymph nodes of the affected extremity. After ... the result of a genetically determined abnormal inflammatory reaction to mineral particles in irritant red clay soils derived ...
In general, it is used to incorporate food ingredients, enzymes, cells or other materials on a micro metric scale. ... Interfacial cross-linking is derived from interfacial polycondensation, and was developed to avoid the use of toxic diamines, ... IUPAC definition Microcapsule: Hollow microparticle composed of a solid shell surrounding a core-forming space available to ... "The Gut Microbiota and Human Health with an Emphasis on the Use of Microencapsulated Bacterial Cells", Journal of Biomedicine ...
One of the most common sources of β(1,3)D-glucan for supplement use is derived from the cell wall of baker's yeast ( ... "Role of the glycocalyx in regulating access of microparticles to apical plasma membranes of intestinal epithelial cells: ... β-Glucans (beta-glucans) comprise a group of β-D-glucose polysaccharides naturally occurring in the cell walls of cereals, ... β-glucans form a natural component of the cell walls of bacteria, fungi, yeast, and cereals such as oat and barley. Each type ...
positive regulation of blood microparticle formation. • negative regulation of endothelial cell proliferation. • positive ... As most information regarding TNF signaling is derived from TNFR1, the role of TNFR2 is likely underestimated. ... but it is produced also by a broad variety of cell types including lymphoid cells, mast cells, endothelial cells, cardiac ... regulation of heterotypic cell-cell adhesion. • negative regulation of mitotic cell cycle. • endothelial cell apoptotic process ...
The word galactose was coined by Charles Weissman[5] in the mid 19th century and is derived from Greek galaktos (milk) and the ... Galactose is a component of the antigens present on blood cells that determine blood type within the ABO blood group system. In ... Consequently, various food products made with dairy-derived ingredients can contain lactose.[9] Galactose metabolism, which ...
positive regulation of macrophage derived foam cell differentiation. • positive regulation of cholesterol storage. • plasma ... positive regulation of blood microparticle formation. • positive regulation of reactive oxygen species metabolic process. ... cell adhesion. • cell surface receptor signaling pathway. • nitric oxide mediated signal transduction. • blood coagulation. • ... cell surface. • membrane. • endocytic vesicle membrane. • platelet alpha granule membrane. • membrane raft. ...
Microparticles of carbon are produced in diesel engine exhaust fumes, and may accumulate in the lungs.[124] In these examples, ... see diamond anvil cell), high-performance bearings, and limited use in specialized windows.[120][121] With the continuing ... the most important energy-transfer molecule in all living cells. ...
The crude feed, a mixture of soluble proteins, contaminants, cells, and cell debris, is then passed upward through the expanded ... Chromatography, pronounced /ˌkroʊməˈtɒɡrəfi/, is derived from Greek χρῶμα chroma, which means "color", and γράφειν graphein, ... Jellema LJ, Markesteijn AP, Westerweel J, Verpoorte E (May 2010). "Tunable hydrodynamic chromatography of microparticles ... Programmed cell death Apoptosis. Pyknosis. Karyorrhexis. Karyolysis. Accumulations. pigment Hemosiderin. Lipochrome/Lipofuscin ...
... normal physiologic coagulation requires the presence of two cell types for formation of coagulation complexes: cells that ... Many analysers are capable of measuring a "derived fibrinogen" level from the graph of the Prothrombin time clot. ... as well as procoagulant microparticles or microvesicles shed from them. Calcium is also required at other points in the ... Mediates cell adhesion. Glomerulopathy with fibronectin deposits antithrombin III. Inhibits IIa, Xa, and other proteases. ...
Cells of interest in such cases are endothelial cells of blood vessels, and leukocytes: *The inflamed blood vessels ... Targeting ligands can be immunogenic, since current targeting ligands used in preclinical experiments are derived from animal ... such as inflamed cells or cancer cells. Current microbubbles in development are composed of a lipid monolayer shell with a ... Cancer: cancer cells also express a specific set of receptors, mainly receptors that encourage angiogenesis, or the growth of ...
Thus laden with high levels of POPs, the microparticles are often ingested by organisms in the biosphere[citation needed]. ... Extensive studies in Europe indicate that the chlorine found in emitted dioxins is not derived from HCl in the flue gases. ... see closed-cell PVC foamboard). Sheets are cut using saws and rotary cutting equipment. Plasticized PVC is also used to produce ... Weathering degradation of plastics results in their surface embrittlement and microcracking, yielding microparticles that ...
... s have no cell nucleus: they are fragments of cytoplasm that are derived from the megakaryocytes[2] of the bone marrow ... "Platelets amplify inflammation in arthritis via collagen-dependent microparticle production". Science. 327 (5965): 580-3. ... Berridge, Michael J. (1 October 2014). "Module 11: Cell Stress, Inflammatory Responses and Cell Death". Cell Signalling Biology ... "Programmed anuclear cell death delimits platelet life span". Cell. 128 (6): 1173-86. doi:10.1016/j.cell.2007.01.037. PMID ...
Secondary cells and batteries containing alkaline or other non-acid electrolytes - Secondary lithium cells and batteries for ... Qi, Zhaoxiang; Koenig, Gary M. (16 August 2016). "High-Performance LiCoO2Sub-Micrometer Materials from Scalable Microparticle ... "Layered Lithium-Manganese Oxide Electrodes Derived from Rock-Salt LixMnyOz (x+y=z) Precursors" 194th Meeting of the ... Battery versus cellEdit. A cell is a basic electrochemical unit that contains the electrodes, separator, and electrolyte.[19][ ...
positive regulation of macrophage derived foam cell differentiation. • regulation of vasoconstriction. • positive regulation of ... blood microparticle. • extracellular space. • cytosol. • extracellular region. Biological process. • uterine smooth muscle ... regulation of cell proliferation. • regulation of cell growth. • positive regulation of cell proliferation. • fibroblast ... cell-cell signaling. • renin-angiotensin regulation of aldosterone production. • aging. • vasodilation. • positive regulation ...
Katie Fehrenbacher New Rechargeable Cell Has 7 Times Higher Energy Density Than Li-ion Cells, Nikkei Technology, 23 July 2014, ... researchers at Nanyang Technological University used a materials derived from a titanium dioxide gel derived from naturally ... "High-Performance LiCoO2Sub-Micrometer Materials from Scalable Microparticle Template Processing". ChemistrySelect. 1 (13): 3992 ... researchers from CMU found that prismatic cells are more likely to benefit from production scaling than cylindrical cells. ...
... that cells in the G2 Phase are more resistant to radiation than cells in the M Phase (Radiosensitivity and Cell cycle). The ... "The interaction of natural background gamma radiation with depleted uranium micro-particles in the human body". Journal of ... Loads and Fate of Fertilizer Derived Uranium. Backhuys Publishers, Leiden, The Netherlands, ISBN 978-90-5782-193-6. Koppe JG, ... According to SET, primary exposure to a beta particle alters a cell to the G2 Phase, which Busby claims would render the cell ...
"Controlled Inhibition of the Mesenchymal Stromal Cell Pro-inflammatory Secretome via Microparticle Engineering". Stem Cell ... working on the development of regenerative therapeutic methods by collecting patient-derived induced pluripotent stem cells ( ... She represents India in the International Stem Cell Initiative (ISCI) and the International Stem Cell Banking Initiative (ISCBI ... Inamdar's group works on the basic biology of stem cells . The group led by her has developed normal stem cell lines from ...
The device for which SPR is derived includes a sensor chip, a flow cell, a light source, a prism, and a fixed angle position ... "SIMAG Basic : Magnetic nano and micro particles". "SIMAG Affinity : Magnetic nano and micro particles by chemicell". "Home - ... cells transfected with cloned receptors, and cells that are either in culture or isolated prior to analysis. Saturation binding ... In this type of assay the binding of a ligand to cells is followed over time. The obtained signal is proportional to the number ...
One of the most common sources of β(1,3)D-glucan for supplement use is derived from the cell wall of baker's yeast ( ... "Role of the glycocalyx in regulating access of microparticles to apical plasma membranes of intestinal epithelial cells: ... β-glucans form a natural component of the cell walls of bacteria, fungi, yeast, and cereals such as oat and barley. Each type ... M cells within the Peyer's patches physically transport the insoluble whole glucan particles into the gut-associated lymphoid ...
... normal physiologic coagulation requires the presence of two cell types for formation of coagulation complexes: cells that ... Many analysers are capable of measuring a "derived fibrinogen" level from the graph of the Prothrombin time clot. If a ... as well as procoagulant microparticles or microvesicles shed from them. Calcium is also required at other points in the ... First is the initiation phase, which occurs in tissue-factor-expressing cells. This is followed by the propagation phase, which ...
2017). "Graft-derived cell-free DNA, a noninvasive early rejection and graft damage marker in liver transplantation: A ... Diehl F, Li M, Kinzler, KW, Vogelstein B, Dressman D (2006). "BEAMing: single-molecule PCR on microparticles in water-in-oil ... 2017). "Single Cell-Based Vector Tracing in Patients with ADA-SCID Treated with Stem Cell Gene Therapy". Molecular Therapy - ... 2016). "Digital Quantification of Proteins and mRNA in Single Mammalian Cells". Molecular Cell. 61 (6): 914-24. doi:10.1016/j. ...
Atherosclerosis refers to a progressive narrowing of the arteries due to atheroma, derived from the Greek word for gruel or ... ISBN 978-0-19-157556-3. Wah TM (August 2017). "Image-guided ablation of renal cell carcinoma". Clinical Radiology. Elsevier BV ... Drug eluting bead transarterial chemoembolization (DEB-TACE): delivery of microparticles that are themselves loaded with the ... Cryoablation Cryoablation is a treatment option that destroys cancer cells by applying extremely cold temperatures at the ...
PDMP, monocyte-derived microparticles (MDMP), and endothelial cell-derived microparticles (EDMP) were detected by flow ... Nomura, S., Ishii, K., Kanazawa, S. et al. Significance of elevation in cell-derived microparticles after allogeneic stem cell ... Significance of elevation in cell-derived microparticles after allogeneic stem cell transplantation: transient elevation of ... Significance of elevation in cell-derived microparticles after allogeneic stem cell transplantation: transient elevation of ...
Cell-derived microparticles (MPs) have been described as vital contributors to the inflammatory process. However, its role in ... Keywords : chronic periodontitis; diabetes; gingival crevicular fluid; cell-derived microparticles. · abstract in Portuguese · ... Cell Derived Microparticles in Gingival Crevicular Fluid from Periodontitis Patients with Type 2 Diabetes. Braz. Dent. J. [ ... Therefore, we aimed to detect the presence neutrophil (CD66b+) and platelet (CD41b+) derived microparticles in gingival ...
A Useful Biomarker for Predicting One-Year Mortality in Patients with Advanced Non-Small Cell Lung Cancer. Chin-Chou Wang,1,2,3 ... platelet-derived apoptotic MPs (PDAp-MPs), (3) endothelial-derived activated MPs (EDAc-MPs), and (4) endothelial-derived ... This study tested the hypothesis that circulating microparticles (MPs) are useful biomarkers for predicting one-year mortality ... Levels of four MPs in circulation, (1) platelet-derived activated MPs (PDAc-MPs), (2) ...
... recently reported to associate with elevated microvesicle/microparticle (MP) counts, have also long been known to be ... Detection and Isolation of Cell-Derived Microparticles Are Compromised by Protein Complexes Resulting From Shared Biophysical ... These data may necessitate reevaluation of certain published data on patient-derived MPs and contribute to correct the clinical ... Numerous diseases, recently reported to associate with elevated microvesicle/microparticle (MP) counts, have also long been ...
To study the contribution of tumor cells to tumor regrowth following therapy, we focused on tumor-derived microparticles (TMPs ... Acute chemotherapy can induce rapid bone-marrow derived pro-angiogenic cell (BMDC) mobilization and tumor homing, contributing ... Tumor-derived microparticles induce bone marrow-derived cell mobilization and tumor homing: a process regulated by osteopontin ... To study the contribution of tumor cells to tumor regrowth following therapy, we focused on tumor-derived microparticles (TMPs ...
The Impact of Sickle Cell Disease Induced Oxidative Stress on MP Proteome ... Analysis of RBC Derived Microparticles from Sickle Cell Mice using Mass Spectrometry: ... Analysis of RBC Derived Microparticles from Sickle Cell Mice using Mass Spectrometry: The Impact of Sickle Cell Disease Induced ... This seminar, will also focus on proteomic differences between Microparticles derived from wildtype and SCD transgenic mouse ...
T cell gate. (C) IFN-γ+ cells and IL-2+ cells (n = 9) within CD45+CD3+CD4+ cells. (D) IFN-γ+ cells and IL-2+ cells (n = 9) ... Reversing drug resistance of soft tumor-repopulating cells by tumor cell-derived chemotherapeutic microparticles. Cell Res. 26 ... B) Lymphocyte infiltrates including CD45+CD3+ T cells, CD45+CD3+CD4+ T cells, CD45+CD3+CD8+ T cells, CD4/CD8 T cell ratio, and ... F) Lymphocyte infiltrates including CD45+CD3+ T cells, CD45+CD3+CD4+ T cells, and CD45+CD3+CD8+ T cells. (G) CD4/CD8 T cell ...
Derived Cells Induced by High‐Fat Diet. ...
Measurement of circulating cell-derived microparticles by flow cytometry: Sources of variability within the assay ... INTRODUCTION: Circulating cell-derived microparticles (MPs) have been implicated in several disease processes and elevated ... INTRODUCTION: Circulating cell-derived microparticles (MPs) have been implicated in several disease processes and elevated ... Measurement of circulating cell-derived microparticles by flow cytometry: Sources of variability within the assay. Thrombosis ...
Red cell-derived microparticles (RMP) as haemostatic agent. Thromb Haemost. 2013;110(4):751-760.. View this article via: PubMed ... Circulating cell membrane microparticles transfer heme to endothelial cells and trigger vasoocclusions in sickle cell disease. ... Nitric oxide scavenging by red blood cell microparticles and cell-free hemoglobin as a mechanism for the red cell storage ... Townes-SS RBC-derived microparticles promote toxicity in human endothelial cells. (A) Oxidative toxicity in human umbilical ...
The absolute counting of red cell-derived microparticles with red cell bead by flow rate based assay ... The absolute counting of red cell-derived microparticles with red cell bead by flow rate based assay ... The absolute counting of red cell-derived microparticles with red cell bead by flow rate based assay ...
Comparison of exosomes secreted by induced pluripotent stem cell-derived mesenchymal stem cells and synovial membrane-derived ... B) Determination of the percentage of CD4+IFNγ+ Th1 cells, CD4+IL17+ Th17 cells, CD4+Foxp3+ Treg cells, CD4+IL10+ Tr1 cells, ... cytotoxic T cells, CD4+IFN-γ+ Th1 cells, CD4+IL-10+ Tr1 cells and CD4+CD25+Foxp3+ Treg cells in splenocytes (n=4) when ... Human mesenchymal stem cell-derived microvesicles modulate T cell response to islet antigen glutamic acid decarboxylase in ...
... derived from kidney-derived mesenchymal stem cells (KMSCs) have recently been reported to ameliorate rarefaction of peritubular ... In vivo administration of KMSC-derived MPs significantly inhibited EndoMT of PTC endothelial cells and improved PTC rarefaction ... Furthermore, administration of KMSC-derived MPs inhibited inflammatory cell infiltration as well as tubulointerstitial fibrosis ... KMSC-derived MPs significantly ameliorated EndoMT and improved in vitro proliferation of TGF-β1 treated HUVEC. ...
... their role in SCA eryptosis and their impact on the genesis of RBC-derived microparticles (RBC-MPs) remains poorly described. ... however their role in SCA eryptosis and their impact on the genesis of RBC-derived microparticles (RBC-MPs) remains poorly ... the effects of SCA/AA-RBC-MPs on human aortic endothelial cell (HAEC) inflammatory phenotype and TLR4 pathway. Eryptosis, RBC- ... the effects of SCA/AA-RBC-MPs on human aortic endothelial cell (HAEC) inflammatory phenotype and TLR4 pathway. Eryptosis, RBC- ...
... and for adhesive cells including adipose-derived stromal cells (ADSCs) and bone marrow-derived mesenchymal stem cells (BMSCs). ... Furthermore, LMWH/P N/MPs can also bind to adhesive cell surfaces, inducing cells and LMWH/P N/MPs-aggregate formation. Those ... cultures of cells including ADSCs and BMSCs using plasma-medium gel with LMWH/P N/MPs exhibited efficient cell proliferation. ... LMWH/P N/MPs can also bind to tissue culture plates and adsorb exogenous GFs or GFs from those cells. The LMWH/P N/MPs-coated ...
CONCLUSION(S) Cell-derived microparticles were raised in normal pregnancy. Recurrent miscarriage seems to be related to ... endothelial and platelet cell activation and/or consumption. An increase in almost all cMP types was observed in PE. A ... OBJECTIVE To assess current studies on the relationship between cell-derived microparticles (cMP) and recurrent miscarriages ( ... Circulating cell-derived microparticles in severe preeclampsia and in fetal growth restriction.. Jaume Alijotas-Reig, Carles ...
Circulating cell-derived microparticles in malaria patients Nantakomon D., Udomsangpetch R., Pattanapunyasat K., Looareesuwan S ...
Cell-Derived Microparticles Promote Coagulation after Moderate Exercise. SOSSDORF, MAIK; OTTO, GORDON P.; CLAUS, RALF A.; More ...
Cell-derived Microparticles. Extracellular membrane vesicles generated by the shedding of CELL MEMBRANES blebs. Microparticles ... Membrane microparticles are submicron fragments of membrane vesicles shed from various cell types. Circulating endothelial ... Microparticles are used in a variety of different fields, such as drug delivery. Recently, non-spherical microparticle ... and other cell types circulate in the peripheral blood and through the MICROVASCULATURE where larger cells cannot, functioning ...
... circulating levels of endothelial cell-derived microparticles and bone marrow-derived endothelial progenitor cells, and indices ... endothelial cell injury, as represented by elevated levels of circulating endothelial cell-derived microparticles (EMPs), and ... Endothelial cell-derived microparticles. EC injury is assessed by measuring circulating EMPs, which are markers of activated or ... Cellular biomarkers of endothelial health: microparticles, endothelial progenitor cells, and circulating endothelial cells. J ...
Improving the dissolution of fenofibrate with yeast cell-derived hollow core/shell carbon microparticles. In: RSC Advances. ... Improving the dissolution of fenofibrate with yeast cell-derived hollow core/shell carbon microparticles. RSC Advances. 2016 ... Improving the dissolution of fenofibrate with yeast cell-derived hollow core/shell carbon microparticles. / Huang, Xiaoxi; ... title = "Improving the dissolution of fenofibrate with yeast cell-derived hollow core/shell carbon microparticles", ...
Circulating Endothelium-Derived Microparticles (EMPs). Preparation of platelet-free plasma. Endothelium-derived microparticles ... endothelial microparticle. EPC. endothelial progenitor cell. FMD. flow-mediated dilation. L-NMMA. LG-monomethyl-arginine. NO. ... 2003) Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis. Thromb ... 2003) Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis. Thromb ...
... monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T ... are excellent vehicles for releasing molecules inside the cell. The aim of this work was to use MSMPs to deliver viral specific ... MHC class I restricted epitopes into human antigen presenting cells ( ... The microparticles are internalized into dendritic cells (DC). (a) Photographs show control cells (MDDCs) or with internalized ...
Neutrophil microparticles-induced injuries and morphological alterations in human umbilical vein endothelial cells (HUVECs) ... In this study we investigated the potential role of myeloperoxidase in the endothelial cell injury caused by neutrophil-derived ... Exposure of HUVEC to neutrophil microparticles induced a loss of cell membrane integrity and morphological changes. The ... in neutrophil microparticles and that the microparticle-associated myeloperoxidase cause injury to endothelial cells. Hence, ...
Cell-cell communication has proven to be even more complex than previously thought since the discovery that extracellular ... Cell-derived microparticles. *Exosomes. *Microrna. Get free article suggestions today. Mendeley saves you time finding and ... Cell-cell communication has proven to be even more complex than previously thought since the discovery that extracellular ... More than simply cell dust, extracellular vesicles are capable of transferring biological information to neighboring cells and ...
Cell-Derived Microparticles in Patients with Type 2 Diabetes Mellitus: a Systematic Review and Meta-Analysis ... Type 2 diabetes mellitus Microparticles Biomarker Platelet-derived microparticle Endothelium-derived microparticle ... Supplementary Material for: Cell-Derived Microparticles in Patients with Type 2 Diabetes Mellitus: a Systematic Review and Meta ... i,Background/Aims: ,/i,The aim of this study was to assess the association between circulating cell-derived microparticles (MPs ...
Circulating cell-derived microparticles (MPs) are sub-micronic phospholipid vesicles derived from cells of different types in ... Cell-derived microparticles (MPs) and their role in unexplained recurrent pregnancy loss * Rucha Kiran Patil National Institute ... There are many studies which show the association of specific cell-derived MPs to different pathological states. MPs act as a ...
Cell-derived microparticles and the lung.. Nieri D, Neri T, Petrini S, Vagaggini B, Paggiaro P, Celi A. ... Tiotropium inhibits proinflammatory microparticle generation by human bronchial and endothelial cells.. Neri T, Scalise V, ... Leptin induces the generation of procoagulant, tissue factor bearing microparticles by human peripheral blood mononuclear cells ... Pirfenidone inhibits p38-mediated generation of procoagulant microparticles by human alveolar epithelial cells. ...
Washing samples results in decreased AnnV+MPs (P=0.002) and platelet-derived MPs (PMPs) (P=0.002). Double centrifugation of MPs ... Circulating cell-derived microparticles (MPs) have been implicated in several disease processes and elevated levels are found ... Cell-Derived Microparticles, Endothelial Cells, Flow Cytometry, Humans, Leukocytes, Middle Aged, Platelet Endothelial Cell ... Measurement of circulating cell-derived microparticles by flow cytometry: sources of variability within the assay. ...
Cell Derived Microparticles in Gingival Crevicular Fluid from Periodontitis Patients with Cell Derived Microparticles in ... Abstract Cell-derived microparticles (MPs) have been described as vital contributors to the inflammatory process. However, its ... Cell-Derived Microparticles Limits: Female / Humans / Male Language: English Journal: Braz. dent. j Journal subject: Dentistry ... Cell-Derived Microparticles Limits: Female / Humans / Male Language: English Journal: Braz. dent. j Journal subject: Dentistry ...

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