Cell derived microparticles. Medical search

Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.

Relating to the size of solids.

Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.

A biocompatible polymer used as a surgical suture material.

The preparation, mixing, and assembling of a drug. (From Remington, The Science and Practice of Pharmacy, 19th ed, p1814)

Forms to which substances are incorporated to improve the delivery and the effectiveness of drugs. Drug carriers are used in drug-delivery systems such as the controlled-release technology to prolong in vivo drug actions, decrease drug metabolism, and reduce drug toxicity. Carriers are also used in designs to increase the effectiveness of drug delivery to the target sites of pharmacological actions. Liposomes, albumin microspheres, soluble synthetic polymers, DNA complexes, protein-drug conjugates, and carrier erythrocytes among others have been employed as biodegradable drug carriers.

A polyester used for absorbable sutures & surgical mesh, especially in ophthalmic surgery. 2-Hydroxy-propanoic acid polymer with polymerized hydroxyacetic acid, which forms 3,6-dimethyl-1,4-dioxane-dione polymer with 1,4-dioxane-2,5-dione copolymer of molecular weight about 80,000 daltons.

Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.

Deacetylated CHITIN, a linear polysaccharide of deacetylated beta-1,4-D-glucosamine. It is used in HYDROGEL and to treat WOUNDS.

Dosage forms of a drug that act over a period of time by controlled-release processes or technology.

A normal intermediate in the fermentation (oxidation, metabolism) of sugar. The concentrated form is used internally to prevent gastrointestinal fermentation. (From Stedman, 26th ed)

Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.

Chemistry dealing with the composition and preparation of agents having PHARMACOLOGIC ACTIONS or diagnostic use.

Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).

Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.

Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.

Structures which are part of the CELL MEMBRANE or have cell membrane as a major part of their structure.

The application of scientific knowledge or technology to pharmacy and the pharmaceutical industry. It includes methods, techniques, and instrumentation in the manufacture, preparation, compounding, dispensing, packaging, and storing of drugs and other preparations used in diagnostic and determinative procedures, and in the treatment of patients.

Hard or soft soluble containers used for the oral administration of medicine.

A series of progressive, overlapping events, triggered by exposure of the PLATELETS to subendothelial tissue. These events include shape change, adhesiveness, aggregation, and release reactions. When carried through to completion, these events lead to the formation of a stable hemostatic plug.

A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.

Constituent composed of protein and phospholipid that is widely distributed in many tissues. It serves as a cofactor with factor VIIa to activate factor X in the extrinsic pathway of blood coagulation.

The process of the interaction of BLOOD COAGULATION FACTORS that results in an insoluble FIBRIN clot.

Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.

Substances made up of an aggregation of small particles, as that obtained by grinding or trituration of a solid drug. In pharmacy it is a form in which substances are administered. (From Dorland, 28th ed)

Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.

Platelet membrane glycoprotein IIb is an integrin alpha subunit that heterodimerizes with INTEGRIN BETA3 to form PLATELET GLYCOPROTEIN GPIIB-IIIA COMPLEX. It is synthesized as a single polypeptide chain which is then postranslationally cleaved and processed into two disulfide-linked subunits of approximately 18 and 110 kDa in size.

Polymerized forms of styrene used as a biocompatible material, especially in dentistry. They are thermoplastic and are used as insulators, for injection molding and casting, as sheets, plates, rods, rigid forms and beads.

A trace element that constitutes about 27.6% of the earth's crust in the form of SILICON DIOXIDE. It does not occur free in nature. Silicon has the atomic symbol Si, atomic number 14, and atomic weight [28.084; 28.086].

Term used to designate tetrahydroxy aldehydic acids obtained by oxidation of hexose sugars, i.e. glucuronic acid, galacturonic acid, etc. Historically, the name hexuronic acid was originally given to ascorbic acid.

Agents that cause clotting.

Anhydride polymers with a repeating structure of RC(=O)OC(=O)R. They readily hydrolyze in water making them useful for DELAYED-ACTION PREPARATIONS.

A sugar acid formed by the oxidation of the C-6 carbon of GLUCOSE. In addition to being a key intermediate metabolite of the uronic acid pathway, glucuronic acid also plays a role in the detoxification of certain drugs and toxins by conjugating with them to form GLUCURONIDES.

Characteristics or attributes of the outer boundaries of objects, including molecules.

Completed forms of the pharmaceutical preparation in which prescribed doses of medication are included. They are designed to resist action by gastric fluids, prevent vomiting and nausea, reduce or alleviate the undesirable taste and smells associated with oral administration, achieve a high concentration of drug at target site, or produce a delayed or long-acting drug effect.

The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)

Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours.

Poly-2-methylpropenoic acids. Used in the manufacture of methacrylate resins and plastics in the form of pellets and granules, as absorbent for biological materials and as filters; also as biological membranes and as hydrogens. Synonyms: methylacrylate polymer; poly(methylacrylate); acrylic acid methyl ester polymer.

Usually inert substances added to a prescription in order to provide suitable consistency to the dosage form. These include binders, matrix, base or diluent in pills, tablets, creams, salves, etc.

Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.

Method of using a polycrystalline powder and Rietveld refinement (LEAST SQUARES ANALYSIS) of X-RAY DIFFRACTION or NEUTRON DIFFRACTION. It circumvents the difficulties of producing single large crystals.

Condition of having pores or open spaces. This often refers to bones, bone implants, or bone cements, but can refer to the porous state of any solid substance.

Differential thermal analysis in which the sample compartment of the apparatus is a differential calorimeter, allowing an exact measure of the heat of transition independent of the specific heat, thermal conductivity, and other variables of the sample.

Salts of alginic acid that are extracted from marine kelp and used to make dental impressions and as absorbent material for surgical dressings.

Platelet membrane glycoprotein complex essential for normal platelet adhesion and clot formation at sites of vascular injury. It is composed of three polypeptides, GPIb alpha, GPIb beta, and GPIX. Glycoprotein Ib functions as a receptor for von Willebrand factor and for thrombin. Congenital deficiency of the GPIb-IX complex results in Bernard-Soulier syndrome. The platelet glycoprotein GPV associates with GPIb-IX and is also absent in Bernard-Soulier syndrome.

Water swollen, rigid, 3-dimensional network of cross-linked, hydrophilic macromolecules, 20-95% water. They are used in paints, printing inks, foodstuffs, pharmaceuticals, and cosmetics. (Grant & Hackh's Chemical Dictionary, 5th ed)

Formation and development of a thrombus or blood clot in the blood vessel.

A complex mixture of PHOSPHOLIPIDS; GLYCOLIPIDS; and TRIGLYCERIDES; with substantial amounts of PHOSPHATIDYLCHOLINES; PHOSPHATIDYLETHANOLAMINES; and PHOSPHATIDYLINOSITOLS, which are sometimes loosely termed as 1,2-diacyl-3-phosphocholines. Lecithin is a component of the CELL MEMBRANE and commercially extracted from SOYBEANS and EGG YOLK. The emulsifying and surfactant properties are useful in FOOD ADDITIVES and for forming organogels (GELS).

The study of MAGNETIC PHENOMENA.

Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.

Colloids formed by the combination of two immiscible liquids such as oil and water. Lipid-in-water emulsions are usually liquid, like milk or lotion. Water-in-lipid emulsions tend to be creams. The formation of emulsions may be aided by amphiphatic molecules that surround one component of the system to form MICELLES.

Colloids with a gaseous dispersing phase and either liquid (fog) or solid (smoke) dispersed phase; used in fumigation or in inhalation therapy; may contain propellant agents.

Antibodies that are chemically bound to a substrate material which renders their location fixed.

A network of cross-linked hydrophilic macromolecules used in biomedical applications.

Removal of moisture from a substance (chemical, food, tissue, etc.).

The administration of drugs by the respiratory route. It includes insufflation into the respiratory tract.

An enzyme formed from PROTHROMBIN that converts FIBRINOGEN to FIBRIN.

A cell line established in 1962 from disaggregated Swiss albino mouse embryos. This fibroblast cell line is extremely popular in research.

Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.

Technique whereby the weight of a sample can be followed over a period of time while its temperature is being changed (usually increased at a constant rate).

Cell adhesion molecule and CD antigen that mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells.

Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.

Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.

A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.

Coating with a metal or alloy by electrolysis.

The design or construction of objects greatly reduced in scale.

The testing of materials and devices, especially those used for PROSTHESES AND IMPLANTS; SUTURES; TISSUE ADHESIVES; etc., for hardness, strength, durability, safety, efficacy, and biocompatibility.

Cell adhesion molecules present on virtually all monocytes, platelets, and granulocytes. CD31 is highly expressed on endothelial cells and concentrated at the junctions between them.

Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.

A cellulose derivative which is a beta-(1,4)-D-glucopyranose polymer. It is used as a bulk laxative and as an emulsifier and thickener in cosmetics and pharmaceuticals and as a stabilizer for reagents.

Suspensions of killed or attenuated microorganisms (bacteria, viruses, fungi, protozoa), antigenic proteins, synthetic constructs, or other bio-molecular derivatives, administered for the prevention, amelioration, or treatment of infectious and other diseases.

A chlorinated hydrocarbon that has been used as an inhalation anesthetic and acts as a narcotic in high concentrations. Its primary use is as a solvent in manufacturing and food technology.

The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.

A product formed from skin, white connective tissue, or bone COLLAGEN. It is used as a protein food adjuvant, plasma substitute, hemostatic, suspending agent in pharmaceutical preparations, and in the manufacturing of capsules and suppositories.

A nonimmunologic, chemically induced type of photosensitivity producing a sometimes vesiculating dermatitis. It results in hyperpigmentation and desquamation of the light-exposed areas of the skin.

High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.

The process by which blood or its components are kept viable outside of the organism from which they are derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).

Derivatives of caproic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a carboxy terminated six carbon aliphatic structure.

Methylester of cellulose. Methylcellulose is used as an emulsifying and suspending agent in cosmetics, pharmaceutics and the chemical industry. It is used therapeutically as a bulk laxative.

Two-phase systems in which one is uniformly dispersed in another as particles small enough so they cannot be filtered or will not settle out. The dispersing or continuous phase or medium envelops the particles of the discontinuous phase. All three states of matter can form colloids among each other.

A type of extracellular vesicle, containing RNA and proteins, that is secreted into the extracellular space by EXOCYTOSIS when MULTIVESICULAR BODIES fuse with the PLASMA MEMBRANE.

Homogeneous liquid preparations that contain one or more chemical substances dissolved, i.e., molecularly dispersed, in a suitable solvent or mixture of mutually miscible solvents. For reasons of their ingredients, method of preparation, or use, they do not fall into another group of products.

The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.

Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.

A property of the surface of an object that makes it stick to another surface.

Endogenous substances, usually proteins, that are involved in the blood coagulation process.

A CHROMATOGRAPHY method using supercritical fluid, usually carbon dioxide under very high pressure (around 73 atmospheres or 1070 psi at room temperature) as the mobile phase. Other solvents are sometimes added as modifiers. This is used both for analytical (SFC) and extraction (SFE) purposes.

Techniques where DNA is delivered directly into organelles at high speed using projectiles coated with nucleic acid, shot from a helium-powered gun (gene gun). One of these techniques involves immunization by DNA VACCINES, which delivers DNA-coated gold beads to the epidermis.

White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).

The giving of drugs, chemicals, or other substances by mouth.

The residual portion of BLOOD that is left after removal of BLOOD CELLS by CENTRIFUGATION without prior BLOOD COAGULATION.

Vesicles that are involved in shuttling cargo from the interior of the cell to the cell surface, from the cell surface to the interior, across the cell or around the cell to various locations.

Transparent, tasteless crystals found in nature as agate, amethyst, chalcedony, cristobalite, flint, sand, QUARTZ, and tridymite. The compound is insoluble in water or acids except hydrofluoric acid.

A disorder of HEMOSTASIS in which there is a tendency for the occurrence of THROMBOSIS.

Manufacturing technology for making microscopic devices in the micrometer range (typically 1-100 micrometers), such as integrated circuits or MEMS. The process usually involves replication and parallel fabrication of hundreds or millions of identical structures using various thin film deposition techniques and carried out in environmentally-controlled clean rooms.

Agents causing contraction of the pupil of the eye. Some sources use the term miotics only for the parasympathomimetics but any drug used to induce miosis is included here.

Inorganic or organic compounds containing trivalent iron.

The quality or state of being wettable or the degree to which something can be wet. This is also the ability of any solid surface to be wetted when in contact with a liquid whose surface tension is reduced so that the liquid spreads over the surface of the solid.

Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

The extent to which the active ingredient of a drug dosage form becomes available at the site of drug action or in a biological medium believed to reflect accessibility to a site of action.

Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)

An anionic surface-active agent used for its wetting properties in industry and used in medicine as an irritant and sclerosing agent for hemorrhoids and varicose veins.

Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Apparatus, devices, or supplies intended for one-time or temporary use.

Tree-like, highly branched, polymeric compounds. They grow three-dimensionally by the addition of shells of branched molecules to a central core. The overall globular shape and presence of cavities gives potential as drug carriers and CONTRAST AGENTS.

Microdevices that combine microfluidics technology with electrical and/or mechanical functions for analyzing very small fluid volumes. They consist of microchannels etched into substrates made of silicon, glass, or polymer using processes similar to photolithography. The test fluids in the channels can then interact with different elements such as electrodes, photodetectors, chemical sensors, pumps, and valves.

The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.

A polyhedral CARBON structure composed of around 60-80 carbon atoms in pentagon and hexagon configuration. They are named after Buckminster Fuller because of structural resemblance to geodesic domes. Fullerenes can be made in high temperature such as arc discharge in an inert atmosphere.

A diuretic and renal diagnostic aid related to sorbitol. It has little significant energy value as it is largely eliminated from the body before any metabolism can take place. It can be used to treat oliguria associated with kidney failure or other manifestations of inadequate renal function and has been used for determination of glomerular filtration rate. Mannitol is also commonly used as a research tool in cell biological studies, usually to control osmolarity.

The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.

The analysis of a chemical substance by inserting a sample into a carrier stream of reagent using a sample injection valve that propels the sample downstream where mixing occurs in a coiled tube, then passes into a flow-through detector and a recorder or other data handling device.

Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.

One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.

Endothelial cells that line venous vessels of the UMBILICAL CORD.

A phthalic indicator dye that appears yellow-green in normal tear film and bright green in a more alkaline medium such as the aqueous humor.

The engulfment and degradation of cells by other cells.

The chemical and physical integrity of a pharmaceutical product.

Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)

Colloids with liquid continuous phase and solid dispersed phase; the term is used loosely also for solid-in-gas (AEROSOLS) and other colloidal systems; water-insoluble drugs may be given as suspensions.

Methods of creating machines and devices.

Derivatives of ACETIC ACID which contain an hydroxy group attached to the methyl carbon.

An involuntary contraction of a muscle or group of muscles. Spasms may involve SKELETAL MUSCLE or SMOOTH MUSCLE.

Nanometer-scale wires made of materials that conduct electricity. They can be coated with molecules such as antibodies that will bind to proteins and other substances.

Established cell cultures that have the potential to propagate indefinitely.

Tablets coated with material that delays release of the medication until after they leave the stomach. (Dorland, 28th ed)

A non-steroidal anti-inflammatory agent with analgesic, anti-inflammatory, and antipyretic properties. It is an inhibitor of cyclooxygenase.

Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.

Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.

The clear, viscous fluid secreted by the SYNOVIAL MEMBRANE. It contains mucin, albumin, fat, and mineral salts and serves to lubricate joints.

The coagulation of tissue by an intense beam of light, including laser (LASER COAGULATION). In the eye it is used in the treatment of retinal detachments, retinal holes, aneurysms, hemorrhages, and malignant and benign neoplasms. (Dictionary of Visual Science, 3d ed)

Tissue factor activity is increased in a combined platelet and microparticle sample from cancer patients. (1/383)

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Microparticles are vectors of paradoxical information in vascular cells including the endothelium: role in health and diseases. (2/383)

Both inflammation and thrombosis can be orchestrated by the interactions between circulating cells, such as leukocytes and platelets, with vascular, endothelial and smooth muscle cells, which, during activation or apoptosis, can release circulating microparticles (MPs). Indeed, MPs are membrane vesicles with procoagulant and proinflammatory properties. MPs are present in blood from healthy individuals and in patients under several pathological states, for instance sepsis, preeclampsia, Crohn's disease and diabetes, strengthening the notion that MPs may play a role in these diseases. Circulating MPs or those generated in vitro from apoptotic T cells display deleterious effects on endothelial and/or vasomotor function. In contrast, MPs might be protective to endothelial cells. We have shown that MPs harboring the morphogen sonic hedgehog may represent a new therapeutic approach against endothelial dysfunction during acute severe endothelial injury. Indeed, these types of MPs induce NO release, decrease production of reactive oxygen species and induce angiogenesis from endothelial cells. This protective role for the endothelium was confirmed also by their in vivo injection in mice in which they were also able to reverse endothelial dysfunction in a model of heart ischemia/reperfusion. On the contrary, MPs from preeclamptic women compared to those from normal pregnant women showed pro-inflammatory properties in the vascular wall inducing vascular hyporeactivity in vessels from humans and mice. These effects were associated with complex interactions between NO and cyclooxygenase systems via endothelial cell activation. Altogether, these findings suggest that MPs can be considered as vectors of biological messages for vascular homeostasis, during immunity and inflammation. (+info)

The role of cell death in the pathogenesis of autoimmune disease: HMGB1 and microparticles as intercellular mediators of inflammation. (3/383)

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The relationship between plasma microparticles and disease manifestations in patients with systemic sclerosis. (4/383)

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Measurement of platelet-derived microparticle levels in the chronic phase of cerebral infarction using an enzyme-linked immunosorbent assay. (5/383)

Assessment of platelet function is a critical component of the treatment and secondary prevention of cerebral infarction, and measurement of platelet-derived microparticle (PDMP) levels using flow cytometry may be a good indicator of platelet function. However, the flow cytometric analysis is not feasible in a variety of clinical situations. The goal of the present study was to measure PDMP levels using an enzyme-linked immunosorbent assay (ELISA) in chronic cerebral infarction patients and to determine the utility of PDMP level measurement for the monitoring of the effect of cilostazol and aspirin. A crossover study was performed using 4-weeks of aspirin (100 mg/day) and 4-weeks of cilostazol (200 mg/day) in 18 patients. PDMP levels were also measured in 20 volunteers as controls. Experiments demonstrated that PDMP levels were significantly higher in chronic cerebral infarction patients (median 8.8 U/ml, interquartile range 5.1-14.9 U/ml, n=18) than in controls (median 5.5 U/ml, interquartile range 5.0-8.2 U/ml, n=20) (P=0.047). PDMP levels did not decrease after therapy with either aspirin (median 10.9 U/ml, interquartile range 6.2-17.9 U/ml, n=12) or cilostazol (median 9.2 U/ml, interquartile range 6.1-14.3 U/ml, n=12) compared with baseline PDMP levels in the 12 patients who completed this trial (median 11.4 U/ml, interquartile range 5.2-23.7 U/ml, n=12). There were no significant differences in PDMP levels between aspirin and cilostazol (P=0.61). In conclusion, PDMP levels as measured by ELISA were increased in patients with chronic cerebral infarction regardless of the anti-platelet therapy. This methodology may be a useful strategy of assessing platelet function in chronic cerebral infarction patients. (+info)

Megakaryocyte-derived microparticles: direct visualization and distinction from platelet-derived microparticles. (6/383)

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Dynamics of circulating microparticles in liver transplant patients. (7/383)

BACKGROUND & AIMS: Microparticles are small membrane vesicles released from the cell plasma membrane, particularly in cell stress, apoptosis and altered cellular viability. Hepatocellular carcinoma (HCC) is a hypervascular neoplasm with high levels of apoptosis and necrosis. We investigated the levels of circulating microparticles of both tumor and endothelial origins in liver transplant patients with hepatitis C (HepC) cirrhosis with and without HCC and compared them with healthy people and patients with partial hepatectomy. METHODS: Using immunolabeling of microparticles of different origin and flow cytometry-based enumeration of microparticles, the levels of circulating microparticles were studied in 8 patients with HepC and 8 patients with both HepC and HCC before and within two weeks after the transplant. RESULTS: The initial levels of circulating microparticles were increased in patients with HepC and HCC as compared to patients with HepC alone. They were also increased in liver transplant patients as compared to patients with partial hepatectomy or healthy people. Levels of circulating microparticles were dynamically changing after the transplant, showing an initial increase with a subsequent decrease by the end of the second week after surgery. In some patients with a complicated clinical outcome, the levels of microparticles were continuously increasing after the surgery. CONCLUSION: The levels of circulating microparticles of endothelial and hepatic origin in liver transplant patients dynamically change after surgery and correlate with the clinical outcome. Perspectively, the levels of circulating microparticles may be used in clinical practice as a marker of the functional status of the transplanted liver. (+info)

Diagnostic role of endothelial microparticles in vasculitis. (8/383)

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Microparticles are derived from many other cell types. Microvesicles and exosomes are formed and released by two slightly ... Microparticles derived from monocytes aggravate atherosclerosis by modulating inflammatory cells. Additionally, microvesicles ... For example, those released from antigen-presenting cells (APCs), such as B cells and dendritic cells, are enriched in proteins ... Once released from their cell of origin, microvesicles interact specifically with cells they recognize by binding to cell-type ...

https://en.wikipedia.org/wiki/Microvesicles

"Macrophages discriminate glycosylation patterns of apoptotic cell-derived microparticles". The Journal of Biological Chemistry ... Although most cells are capable of phagocytosis, some cell types perform it as part of their main function. These are called ' ... One of the features of an apoptotic cell is the presentation of a variety of intracellular molecules on the cell surface, such ... Phagocytosis (from Ancient Greek φαγεῖν (phagein) 'to eat', and κύτος, (kytos) 'cell') is the process by which a cell uses its ...

https://en.wikipedia.org/wiki/Phagocytosis

It is derived from the bacteria that causes acne and targets myeloid cells through TLR9 and NOD2. In one of its first rounds of ... MIS416 is a microparticle derived from the cytoskeleton of P. acnes, a species of bacteria present on the skin of most adults ... Bacteria-derived microparticles have several advantages over traditional adjuvants related both to their size and biological ... a non-toxic microparticle adjuvant derived from Propionibacterium acnes comprising immunostimulatory muramyl dipeptide and ...

https://en.wikipedia.org/wiki/MIS416

Kumacheva has made strong contributions in the area of self-assembly of nanoparticles and microparticles. Her research ... affect necrotic heart tissue as well as deter the metastasis of cancer cells. ... interests include nature-derived nanopartocles, e.g., cellulose nanocrystals and nanofibers ). Eugenia Kumacheva's awards and ... Kumacheva's work is the creation of hydrogels and various other chemical environments to either support the life of a stem cell ...

https://en.wikipedia.org/wiki/Eugenia_Kumacheva

MIS416, a non-toxic microparticle adjuvant derived from Propionibacterium acnes comprising immunostimulatory muramyl dipeptide ... and Th17 cells in EAE, and [increased] the serum levels of IFN-γ and IFN-γ-associated proteins in 19 patients with SPMS." " ... "a myeloid-directed microparticle immune response modifier (derived from Cutibacterium acnes), which was originally developed as ... a non-toxic microparticle adjuvant derived from Propionibacterium acnes comprising immunostimulatory muramyl dipeptide and ...

https://en.wikipedia.org/wiki/Innate_Immunotherapeutics

"Detection of fetal DNA and RNA in placenta-derived syncytiotrophoblast microparticles generated in vitro". Clinical Chemistry. ... Cell-free fetal DNA (cffDNA) is fetal DNA that circulates freely in the maternal blood. Maternal blood is sampled by ... Approximately 11 to 13.4 percent of the cell-free DNA in maternal blood is of fetal origin. The amount varies widely from one ... Smaller fragments can represent up to seventy percent of the total cell free DNA in the maternal blood sample. In real-time PCR ...

https://en.wikipedia.org/wiki/Cell-free_fetal_DNA

... the HIV co-receptors CCR5 and CXCR4 could be transferred from an HIV-susceptible cell to a refractory cell by "microparticles ... A clinical trial of dendritic cell-derived EVs was performed in France just before the turn of the century.[citation needed] ... Since apoptotic cells tend to display phosphatidylserine (PS) in the outer bilayer of the cell membrane, apoptotic bodies tend ... They carry a cargo of proteins, nucleic acids, lipids, metabolites, and even organelles from the parent cell. Most cells that ...

https://en.wikipedia.org/wiki/Extracellular_vesicle

Caveolae Cellular dewetting Endothelial activation Endothelial microparticle Endothelial progenitor cell Endothelium-derived ... Endothelial cells in direct contact with blood are called vascular endothelial cells whereas those in direct contact with lymph ... Endothelial cells actively signal to white blood cells of the immune system during inflammation Formation of new blood vessels ... Vascular endothelial cells line the entire circulatory system, from the heart to the smallest capillaries. These cells have ...

https://en.wikipedia.org/wiki/Endothelium

Martínez, MC; Tesse, A; Zobairi, F; Andriantsitohaina, R. Shed membrane microparticles from circulating and vascular cells in ... monocyte-derived and tissue factor-carrying circulating microparticles are related to acute myocardial infarction severity.. ... 胞漿顆粒（英语：Granule (cell biology)）. 黑色素體 · 微粒體 · 過氧化物酶體 · 乙醛酸循環體 · 韋伯潘力氏小體（英语：Weibel-Palade body） ... A whole-cell electron tomography model of vacuole biogenesis in Arabidopsis root cells.. Nature plants

https://zh.wikipedia.org/wiki/囊泡

"Calculation of Single Cell Assimilation Rates From SIP-NanoSIMS-Derived Isotope Ratios: A Comprehensive Approach". Frontiers in ... NanoSIMS can also be used to examine the elemental and isotopic composition of microparticles preserved in the rock record. The ... The mouse gut microbiome was investigated to determine which microbes fed on host-derived compounds. For this, mice were given ... Peteranderl, R.; Lechene, C. (2004-04-01). "Measure of carbon and nitrogen stable isotope ratios in cultured cells". Journal of ...

https://en.wikipedia.org/wiki/Nanoscale_secondary_ion_mass_spectrometry

In many plants, the laticiferous system is formed from rows of cells laid down in the meristem of the stem or root. The cell ... Since the 17th century, latex has been used as a term for the fluid substance in plants, deriving from the Latin word for " ... Latex is a stable dispersion (emulsion) of polymer microparticles in water. Latexes are found in nature, but synthetic latexes ... In the mature plant, the entire laticiferous system is descended from a single cell or group of cells present in the embryo. ...

https://en.wikipedia.org/wiki/Latex

Agarose is a polysaccharide derived from seaweed used for nanoencapsulation of cells and the cell/agarose suspension can be ... Alginate microparticles loaded with myoblast cells and functionalized with RGD allowed control over the growth and ... autologous cells), from another donor (allogeneic cells) or from other species (xenogeneic cells). The use of autologous cells ... The cell type chosen for this technique depends on the desired application of the cell microcapsules. The cells put into the ...

https://en.wikipedia.org/wiki/Cell_encapsulation

Moving into the cell membrane is the transmembrane domain, which is followed by the spacer domain located outside of the cell, ... "Macrophage scavenger receptors and host-derived ligands". Methods. 43 (3): 207-17. doi:10.1016/j.ymeth.2007.06.004. PMID ... "Therapeutic inflammatory monocyte modulation using immune-modifying microparticles". Science Translational Medicine. 6 (219): ... When ligand binds to MARCO on dendritic cells, the cytoskeleton of the cell is altered, allowing for the formation of the long ...

https://en.wikipedia.org/wiki/MARCO

... dioxide Cotton Charcoal Smoke particulates Air pollutants Cosmetics-derived microparticles Drug nanoparticles Implant-derived ... Cells undergo cell death via three main mechanisms: nectoptosis via RIPK1, FADD, RIPK3, and MLKL, ferroptosis via GPX4 ... Necroinflammation is an autoamplifying process where crystals are toxic to cells (cytotoxicity) and cause cell death (necrosis ... form foam cells and giant cells, and try to do extracellular digestion in a process called frustrated phagocytosis. Crystals do ...

https://en.wikipedia.org/wiki/Crystallopathy

When used as scaffolds, hydrogels may contain human cells to repair tissue. They mimic 3D microenvironment of cells. Hydrogel- ... Other additives, such as nanoparticles and microparticles, have been shown to significantly modify the stiffness and gelation ... "The use of chitosan based hydrogel for enhancing the therapeutic benefits of adipose-derived MSCs for acute kidney injury". ... coated wells have been used for cell culture. Investigating biomechanical functions in cells when combined with Holotomography ...

https://en.wikipedia.org/wiki/Hydrogel

"3.3 A cryo-EM structure of a nonenveloped virus reveals a priming mechanism for cell entry". Cell. 141 (3): 472-82. doi:10.1016 ... and micro-particles gave rise to a detailed publication, Ref. Degueldre et al (2006).[] Since 2010 the interest for SP ICP-MS ... "Dynamics in cryo EM reconstructions visualized with maximum-likelihood derived variance maps". Journal of Structural Biology. ... Å resolution CryoTEM structure of β-galactosidase in complex with a cell-permeant inhibitor". Science. 348 (6239): 1147-1151. ...

https://en.wikipedia.org/wiki/Single_particle_analysis

"Encapsulating bacteria in agarose microparticles using microfluidics for high-throughput cell analysis and isolation". ACS ... More recent advancements in microfluidic particles allowed the synthesis of nanometer sized particles from biologically derived ... Hydrocarbon oil is typically not used in cell microfluidic research because it is not compatible with cells and damages cell ... Droplets containing cells can be stored off-chip in PTFE tubing for up to several days while maintaining cell viability and ...

https://en.wikipedia.org/wiki/Droplet-based_microfluidics

... but some compounds from the cell wall are unique and are used to trace bacterial derived DOC (e.g., peptidoglycan). These ... Self-organization of dissolved organic matter tomicelle-like microparticles in river water. Nature 422, 150-154. doi: 10.1038/ ... Bacteria are often viewed as the main consumers of DOC, but they can also produce DOC during cell division and viral lysis. The ... The lytic cycle causes disruption of the cell(s) and release of DOC. Marine macrophytes (i.e., macroalgae and seagrass) are ...

https://en.wikipedia.org/wiki/Dissolved_organic_carbon

A theoretical expression for this spectrum is derived in the following, which can then be fitted to the experimental curve in ... Other applications are the rheology of soft matter, and studies of force-regulated processes in living cells. Forces are ... A magnetic tweezers apparatus consists of magnetic micro-particles, which can be manipulated with the help of an external ... In experiments, the molecule of interest is attached to a magnetic microparticle. The magnetic tweezer is equipped with magnets ...

https://en.wikipedia.org/wiki/Magnetic_tweezers

These cells, together with other immune cells such as macrophages, lymphocytes, neutrophils, mast cells, dendritic cells and ... The word "synovium" is derived from the word "synovia" (or synovial fluid), which is a clear, viscous fluid produced by the ... 2010). "Platelets Amplify Inflammation in Arthritis via Collagen-Dependent Microparticle Production". Science. 327 (5965): 580- ... These hallmark features of FLS in RA are divided into 7 cell-intrinsic hallmarks and 4 cell-extrinsic hallmarks. The cell- ...

https://en.wikipedia.org/wiki/Fibroblast-like_synoviocyte

Cancer cells may facilitate export of PAI-2 via microparticles.[19] PAI-2 provides protection for cancer cells against plasmin- ... "Cloning and expression of a cDNA coding for a human monocyte-derived plasminogen activator inhibitor". Proceedings of the ... Among immune cells, macrophages are the main producers of PAI-2, as both B-cells and T-cells do not produce significant amounts ... "Cell. 156 (5): 1002-16. doi:10.1016/j.cell.2014.01.040. PMC 3988473. PMID 24581498.. ...

https://en.wikipedia.org/wiki/Plasminogen_activator_inhibitor-2

... has shown to induce apoptosis and promote antitumor actions on various cancer cell lines in vitro. Hinokinin has been ... Hinokinin is a dibenzylbutyrolactone lignan, derived from various species of plants. It is a potential antichagonistic agent ... microparticles for Chagas disease". Parasitology Research. 106 (3): 703-708. doi:10.1007/s00436-010-1725-1. ISSN 0932-0113. ...

https://en.wikipedia.org/wiki/Hinokinin

A thick SEI layer results in a higher cell resistance, which decreases cell efficiency. The SEI layer on silicon is composed of ... These microparticles reached an energy density of 3,300 mAh/g. In 2015, Tesla founder Elon Musk claimed that silicon in Model S ... Polymer derived SiOC integrated with graphene aerogel as highly stable Li-ion battery anodes ACS Appl. Mater. Interfaces 2020, ... Lithium-silicon batteries also include cell configurations where Si is in compounds that may at low voltage store lithium by a ...

https://en.wikipedia.org/wiki/Lithium–silicon_battery

... antimetastatic activity of dendritic cells based on vaccines when mechanically heterogenized microparticles of tumor cells are ... One such model, the patient derived tumour xenograft, has shown excellent utility in preserving tumour heterogeneity whilst ... The cancer stem cell model asserts that within a population of tumour cells, there is only a small subset of cells that are ... There are two models used to explain the heterogeneity of tumour cells. These are the cancer stem cell model and the clonal ...

https://en.wikipedia.org/wiki/Tumour_heterogeneity

Cross-priming occurs when a bone marrow-derived cell presents peptides from proteins synthesised in another cell in the context ... that has been absorbed onto gold or tungsten microparticles into the target cells, using compressed helium as an accelerant. ... Studies using chimeric mice have shown that antigen is presented by bone-marrow derived cells, which include dendritic cells, ... The type of T-cell help raised is determined by the differentiated state of antigen presenting cells. Dendritic cells can ...

https://en.wikipedia.org/wiki/DNA_vaccine

Contaminants inside the cells can defeat these safety devices. Also, these features can not be applied to all kinds of cells, e ... Polymer-Derived SiOC Integrated with a Graphene Aerogel As a Highly Stable Li-Ion Battery Anode ACS Appl. Mater. Interfaces ... Qi, Zhaoxiang; Koenig, Gary M. (16 August 2016). "High-Performance LiCoO2Sub-Micrometer Materials from Scalable Microparticle ... The 21700 cell has 50% more energy than the 18650 cell, and the bigger size reduces heat transfer to its surroundings. The ...

https://en.wikipedia.org/wiki/Lithium-ion_battery

Using light microscopy, he observed macrophage cells laden with micro-particles in lymph nodes of the affected extremity. After ... the result of a genetically determined abnormal inflammatory reaction to mineral particles in irritant red clay soils derived ...

https://en.wikipedia.org/wiki/Podoconiosis

In general, it is used to incorporate food ingredients, enzymes, cells or other materials on a micro metric scale. ... Interfacial cross-linking is derived from interfacial polycondensation, and was developed to avoid the use of toxic diamines, ... IUPAC definition Microcapsule: Hollow microparticle composed of a solid shell surrounding a core-forming space available to ... "The Gut Microbiota and Human Health with an Emphasis on the Use of Microencapsulated Bacterial Cells", Journal of Biomedicine ...

https://en.wikipedia.org/wiki/Micro-encapsulation

One of the most common sources of β(1,3)D-glucan for supplement use is derived from the cell wall of baker's yeast ( ... "Role of the glycocalyx in regulating access of microparticles to apical plasma membranes of intestinal epithelial cells: ... β-Glucans (beta-glucans) comprise a group of β-D-glucose polysaccharides naturally occurring in the cell walls of cereals, ... β-glucans form a natural component of the cell walls of bacteria, fungi, yeast, and cereals such as oat and barley. Each type ...

https://en.wikipedia.org/wiki/Beta-glucans

positive regulation of blood microparticle formation. • negative regulation of endothelial cell proliferation. • positive ... As most information regarding TNF signaling is derived from TNFR1, the role of TNFR2 is likely underestimated. ... but it is produced also by a broad variety of cell types including lymphoid cells, mast cells, endothelial cells, cardiac ... regulation of heterotypic cell-cell adhesion. • negative regulation of mitotic cell cycle. • endothelial cell apoptotic process ...

https://en.wikipedia.org/wiki/TNF-alpha

The word galactose was coined by Charles Weissman[5] in the mid 19th century and is derived from Greek galaktos (milk) and the ... Galactose is a component of the antigens present on blood cells that determine blood type within the ABO blood group system. In ... Consequently, various food products made with dairy-derived ingredients can contain lactose.[9] Galactose metabolism, which ...

https://en.m.wikipedia.org/wiki/Galactose

positive regulation of macrophage derived foam cell differentiation. • positive regulation of cholesterol storage. • plasma ... positive regulation of blood microparticle formation. • positive regulation of reactive oxygen species metabolic process. ... cell adhesion. • cell surface receptor signaling pathway. • nitric oxide mediated signal transduction. • blood coagulation. • ... cell surface. • membrane. • endocytic vesicle membrane. • platelet alpha granule membrane. • membrane raft. ...

https://ru.wikipedia.org/wiki/CD36

Microparticles of carbon are produced in diesel engine exhaust fumes, and may accumulate in the lungs.[124] In these examples, ... see diamond anvil cell), high-performance bearings, and limited use in specialized windows.[120][121] With the continuing ... the most important energy-transfer molecule in all living cells. ...

https://en.wikipedia.org/wiki/Carbonaceous

The crude feed, a mixture of soluble proteins, contaminants, cells, and cell debris, is then passed upward through the expanded ... Chromatography, pronounced /ˌkroʊməˈtɒɡrəfi/, is derived from Greek χρῶμα chroma, which means "color", and γράφειν graphein, ... Jellema LJ, Markesteijn AP, Westerweel J, Verpoorte E (May 2010). "Tunable hydrodynamic chromatography of microparticles ... Programmed cell death Apoptosis. Pyknosis. Karyorrhexis. Karyolysis. Accumulations. pigment Hemosiderin. Lipochrome/Lipofuscin ...

https://en.wikipedia.org/wiki/Chromatography

... normal physiologic coagulation requires the presence of two cell types for formation of coagulation complexes: cells that ... Many analysers are capable of measuring a "derived fibrinogen" level from the graph of the Prothrombin time clot. ... as well as procoagulant microparticles or microvesicles shed from them. Calcium is also required at other points in the ... Mediates cell adhesion. Glomerulopathy with fibronectin deposits antithrombin III. Inhibits IIa, Xa, and other proteases. ...

https://en.m.wikipedia.org/wiki/Blood_coagulation

Cells of interest in such cases are endothelial cells of blood vessels, and leukocytes: *The inflamed blood vessels ... Targeting ligands can be immunogenic, since current targeting ligands used in preclinical experiments are derived from animal ... such as inflamed cells or cancer cells. Current microbubbles in development are composed of a lipid monolayer shell with a ... Cancer: cancer cells also express a specific set of receptors, mainly receptors that encourage angiogenesis, or the growth of ...

https://en.wikipedia.org/wiki/Contrast-enhanced_ultrasound

Thus laden with high levels of POPs, the microparticles are often ingested by organisms in the biosphere[citation needed]. ... Extensive studies in Europe indicate that the chlorine found in emitted dioxins is not derived from HCl in the flue gases. ... see closed-cell PVC foamboard). Sheets are cut using saws and rotary cutting equipment. Plasticized PVC is also used to produce ... Weathering degradation of plastics results in their surface embrittlement and microcracking, yielding microparticles that ...

https://en.wikipedia.org/wiki/Vinyl_

... s have no cell nucleus: they are fragments of cytoplasm that are derived from the megakaryocytes[2] of the bone marrow ... "Platelets amplify inflammation in arthritis via collagen-dependent microparticle production". Science. 327 (5965): 580-3. ... Berridge, Michael J. (1 October 2014). "Module 11: Cell Stress, Inflammatory Responses and Cell Death". Cell Signalling Biology ... "Programmed anuclear cell death delimits platelet life span". Cell. 128 (6): 1173-86. doi:10.1016/j.cell.2007.01.037. PMID ...

https://en.wikipedia.org/wiki/Thrombocytopathy

Secondary cells and batteries containing alkaline or other non-acid electrolytes - Secondary lithium cells and batteries for ... Qi, Zhaoxiang; Koenig, Gary M. (16 August 2016). "High-Performance LiCoO2Sub-Micrometer Materials from Scalable Microparticle ... "Layered Lithium-Manganese Oxide Electrodes Derived from Rock-Salt LixMnyOz (x+y=z) Precursors" 194th Meeting of the ... Battery versus cellEdit. A cell is a basic electrochemical unit that contains the electrodes, separator, and electrolyte.[19][ ...

https://en.m.wikipedia.org/wiki/Lithium-ion_batteries

positive regulation of macrophage derived foam cell differentiation. • regulation of vasoconstriction. • positive regulation of ... blood microparticle. • extracellular space. • cytosol. • extracellular region. Biological process. • uterine smooth muscle ... regulation of cell proliferation. • regulation of cell growth. • positive regulation of cell proliferation. • fibroblast ... cell-cell signaling. • renin-angiotensin regulation of aldosterone production. • aging. • vasodilation. • positive regulation ...

https://en.m.wikipedia.org/wiki/Angiotensin

Katie Fehrenbacher New Rechargeable Cell Has 7 Times Higher Energy Density Than Li-ion Cells, Nikkei Technology, 23 July 2014, ... researchers at Nanyang Technological University used a materials derived from a titanium dioxide gel derived from naturally ... "High-Performance LiCoO2Sub-Micrometer Materials from Scalable Microparticle Template Processing". ChemistrySelect. 1 (13): 3992 ... researchers from CMU found that prismatic cells are more likely to benefit from production scaling than cylindrical cells. ...

https://en.wikipedia.org/wiki/Research_in_lithium-ion_batteries

... that cells in the G2 Phase are more resistant to radiation than cells in the M Phase (Radiosensitivity and Cell cycle). The ... "The interaction of natural background gamma radiation with depleted uranium micro-particles in the human body". Journal of ... Loads and Fate of Fertilizer Derived Uranium. Backhuys Publishers, Leiden, The Netherlands, ISBN 978-90-5782-193-6. Koppe JG, ... According to SET, primary exposure to a beta particle alters a cell to the G2 Phase, which Busby claims would render the cell ...

https://en.wikipedia.org/wiki/Christopher_Busby

"Controlled Inhibition of the Mesenchymal Stromal Cell Pro-inflammatory Secretome via Microparticle Engineering". Stem Cell ... working on the development of regenerative therapeutic methods by collecting patient-derived induced pluripotent stem cells ( ... She represents India in the International Stem Cell Initiative (ISCI) and the International Stem Cell Banking Initiative (ISCBI ... Inamdar's group works on the basic biology of stem cells . The group led by her has developed normal stem cell lines from ...

https://en.wikipedia.org/wiki/Maneesha_S._Inamdar

The device for which SPR is derived includes a sensor chip, a flow cell, a light source, a prism, and a fixed angle position ... "SIMAG Basic : Magnetic nano and micro particles". "SIMAG Affinity : Magnetic nano and micro particles by chemicell". "Home - ... cells transfected with cloned receptors, and cells that are either in culture or isolated prior to analysis. Saturation binding ... In this type of assay the binding of a ligand to cells is followed over time. The obtained signal is proportional to the number ...

https://en.wikipedia.org/wiki/Ligand_binding_assay

One of the most common sources of β(1,3)D-glucan for supplement use is derived from the cell wall of baker's yeast ( ... "Role of the glycocalyx in regulating access of microparticles to apical plasma membranes of intestinal epithelial cells: ... β-glucans form a natural component of the cell walls of bacteria, fungi, yeast, and cereals such as oat and barley. Each type ... M cells within the Peyer's patches physically transport the insoluble whole glucan particles into the gut-associated lymphoid ...

https://en.wikipedia.org/wiki/Beta-glucan

... normal physiologic coagulation requires the presence of two cell types for formation of coagulation complexes: cells that ... Many analysers are capable of measuring a "derived fibrinogen" level from the graph of the Prothrombin time clot. If a ... as well as procoagulant microparticles or microvesicles shed from them. Calcium is also required at other points in the ... First is the initiation phase, which occurs in tissue-factor-expressing cells. This is followed by the propagation phase, which ...

https://en.wikipedia.org/wiki/Coagulation

2017). "Graft-derived cell-free DNA, a noninvasive early rejection and graft damage marker in liver transplantation: A ... Diehl F, Li M, Kinzler, KW, Vogelstein B, Dressman D (2006). "BEAMing: single-molecule PCR on microparticles in water-in-oil ... 2017). "Single Cell-Based Vector Tracing in Patients with ADA-SCID Treated with Stem Cell Gene Therapy". Molecular Therapy - ... 2016). "Digital Quantification of Proteins and mRNA in Single Mammalian Cells". Molecular Cell. 61 (6): 914-24. doi:10.1016/j. ...

https://en.wikipedia.org/wiki/Digital_polymerase_chain_reaction

Atherosclerosis refers to a progressive narrowing of the arteries due to atheroma, derived from the Greek word for gruel or ... ISBN 978-0-19-157556-3. Wah TM (August 2017). "Image-guided ablation of renal cell carcinoma". Clinical Radiology. Elsevier BV ... Drug eluting bead transarterial chemoembolization (DEB-TACE): delivery of microparticles that are themselves loaded with the ... Cryoablation Cryoablation is a treatment option that destroys cancer cells by applying extremely cold temperatures at the ...

https://en.wikipedia.org/wiki/Interventional_radiology

PDMP, monocyte-derived microparticles (MDMP), and endothelial cell-derived microparticles (EDMP) were detected by flow ... Nomura, S., Ishii, K., Kanazawa, S. et al. Significance of elevation in cell-derived microparticles after allogeneic stem cell ... Significance of elevation in cell-derived microparticles after allogeneic stem cell transplantation: transient elevation of ... Significance of elevation in cell-derived microparticles after allogeneic stem cell transplantation: transient elevation of ...

https://www.nature.com/articles/1705150?error=cookies_not_supported&code=930b43be-650d-416a-8336-6694b5111723

Cell-derived microparticles (MPs) have been described as vital contributors to the inflammatory process. However, its role in ... Keywords : chronic periodontitis; diabetes; gingival crevicular fluid; cell-derived microparticles. · abstract in Portuguese · ... Cell Derived Microparticles in Gingival Crevicular Fluid from Periodontitis Patients with Type 2 Diabetes. Braz. Dent. J. [ ... Therefore, we aimed to detect the presence neutrophil (CD66b+) and platelet (CD41b+) derived microparticles in gingival ...

http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0103-64402017000600675&lng=en&nrm=iso&tlng=en

A Useful Biomarker for Predicting One-Year Mortality in Patients with Advanced Non-Small Cell Lung Cancer. Chin-Chou Wang,1,2,3 ... platelet-derived apoptotic MPs (PDAp-MPs), (3) endothelial-derived activated MPs (EDAc-MPs), and (4) endothelial-derived ... This study tested the hypothesis that circulating microparticles (MPs) are useful biomarkers for predicting one-year mortality ... Levels of four MPs in circulation, (1) platelet-derived activated MPs (PDAc-MPs), (2) ...

https://www.hindawi.com/journals/bmri/2014/173401/abs/

We propose that these microparticles contribute to the local hypercoagulation and fibrin deposition in inflamed joints of ... Synovial fluid contains high numbers of microparticles derived from leukocytes that are strongly coagulant via the factor VII- ... Cell-derived microparticles in synovial fluid from inflamed arthritic joints support coagulation exclusively via a factor VII- ... Conclusion: Synovial fluid contains high numbers of microparticles derived from leukocytes that are strongly coagulant via the ...

https://pubmed.ncbi.nlm.nih.gov/12428225/?dopt=Abstract

... recently reported to associate with elevated microvesicle/microparticle (MP) counts, have also long been known to be ... Detection and Isolation of Cell-Derived Microparticles Are Compromised by Protein Complexes Resulting From Shared Biophysical ... These data may necessitate reevaluation of certain published data on patient-derived MPs and contribute to correct the clinical ... Numerous diseases, recently reported to associate with elevated microvesicle/microparticle (MP) counts, have also long been ...

https://pubmed.ncbi.nlm.nih.gov/21041717/

The Impact of Sickle Cell Disease Induced Oxidative Stress on MP Proteome ... Analysis of RBC Derived Microparticles from Sickle Cell Mice using Mass Spectrometry: ... Analysis of RBC Derived Microparticles from Sickle Cell Mice using Mass Spectrometry: The Impact of Sickle Cell Disease Induced ... This seminar, will also focus on proteomic differences between Microparticles derived from wildtype and SCD transgenic mouse ...

https://videocast.nih.gov/Summary.asp?File=23799

T cell gate. (C) IFN-γ+ cells and IL-2+ cells (n = 9) within CD45+CD3+CD4+ cells. (D) IFN-γ+ cells and IL-2+ cells (n = 9) ... Reversing drug resistance of soft tumor-repopulating cells by tumor cell-derived chemotherapeutic microparticles. Cell Res. 26 ... B) Lymphocyte infiltrates including CD45+CD3+ T cells, CD45+CD3+CD4+ T cells, CD45+CD3+CD8+ T cells, CD4/CD8 T cell ratio, and ... F) Lymphocyte infiltrates including CD45+CD3+ T cells, CD45+CD3+CD4+ T cells, and CD45+CD3+CD8+ T cells. (G) CD4/CD8 T cell ...

https://stm.sciencemag.org/content/11/474/eaat5690

Derived Cells Induced by High‐Fat Diet. ...

https://insights.ovid.com/pubmed?PMID=29080294

Measurement of circulating cell-derived microparticles by flow cytometry: Sources of variability within the assay ... INTRODUCTION: Circulating cell-derived microparticles (MPs) have been implicated in several disease processes and elevated ... INTRODUCTION: Circulating cell-derived microparticles (MPs) have been implicated in several disease processes and elevated ... Measurement of circulating cell-derived microparticles by flow cytometry: Sources of variability within the assay. Thrombosis ...

https://www.zora.uzh.ch/id/eprint/44606/

Red cell-derived microparticles (RMP) as haemostatic agent. Thromb Haemost. 2013;110(4):751-760.. View this article via: PubMed ... Circulating cell membrane microparticles transfer heme to endothelial cells and trigger vasoocclusions in sickle cell disease. ... Nitric oxide scavenging by red blood cell microparticles and cell-free hemoglobin as a mechanism for the red cell storage ... Townes-SS RBC-derived microparticles promote toxicity in human endothelial cells. (A) Oxidative toxicity in human umbilical ...

https://insight.jci.org/articles/view/120451

The absolute counting of red cell-derived microparticles with red cell bead by flow rate based assay ... The absolute counting of red cell-derived microparticles with red cell bead by flow rate based assay ... The absolute counting of red cell-derived microparticles with red cell bead by flow rate based assay ...

https://www.tropmedres.ac/publications/133537

Keywords: Microparticles, Acute Coronary Syndromes, Endothelium-derived Microparticles, Platelet-derived Microparticles, Acute ... Keywords:Microparticles, Acute Coronary Syndromes, Endothelium-derived Microparticles, Platelet-derived Microparticles, Acute ... Cell-Derived Microparticles and Acute Coronary Syndromes: Is there a Predictive Role for Microparticles?. Author(s): Effimia ... Title:Cell-Derived Microparticles and Acute Coronary Syndromes: Is there a Predictive Role for Microparticles? ...

https://www.eurekaselect.com/177430/article

Comparison of exosomes secreted by induced pluripotent stem cell-derived mesenchymal stem cells and synovial membrane-derived ... B) Determination of the percentage of CD4+IFNγ+ Th1 cells, CD4+IL17+ Th17 cells, CD4+Foxp3+ Treg cells, CD4+IL10+ Tr1 cells, ... cytotoxic T cells, CD4+IFN-γ+ Th1 cells, CD4+IL-10+ Tr1 cells and CD4+CD25+Foxp3+ Treg cells in splenocytes (n=4) when ... Human mesenchymal stem cell-derived microvesicles modulate T cell response to islet antigen glutamic acid decarboxylase in ...

https://www.thno.org/v08p1399.htm

... derived from kidney-derived mesenchymal stem cells (KMSCs) have recently been reported to ameliorate rarefaction of peritubular ... In vivo administration of KMSC-derived MPs significantly inhibited EndoMT of PTC endothelial cells and improved PTC rarefaction ... Furthermore, administration of KMSC-derived MPs inhibited inflammatory cell infiltration as well as tubulointerstitial fibrosis ... KMSC-derived MPs significantly ameliorated EndoMT and improved in vitro proliferation of TGF-β1 treated HUVEC. ...

https://stemcellres.biomedcentral.com/articles/10.1186/s13287-015-0012-6

... their role in SCA eryptosis and their impact on the genesis of RBC-derived microparticles (RBC-MPs) remains poorly described. ... however their role in SCA eryptosis and their impact on the genesis of RBC-derived microparticles (RBC-MPs) remains poorly ... the effects of SCA/AA-RBC-MPs on human aortic endothelial cell (HAEC) inflammatory phenotype and TLR4 pathway. Eryptosis, RBC- ... the effects of SCA/AA-RBC-MPs on human aortic endothelial cell (HAEC) inflammatory phenotype and TLR4 pathway. Eryptosis, RBC- ...

https://www.frontiersin.org/articles/10.3389/fimmu.2020.551441/full

... and for adhesive cells including adipose-derived stromal cells (ADSCs) and bone marrow-derived mesenchymal stem cells (BMSCs). ... Furthermore, LMWH/P N/MPs can also bind to adhesive cell surfaces, inducing cells and LMWH/P N/MPs-aggregate formation. Those ... cultures of cells including ADSCs and BMSCs using plasma-medium gel with LMWH/P N/MPs exhibited efficient cell proliferation. ... LMWH/P N/MPs can also bind to tissue culture plates and adsorb exogenous GFs or GFs from those cells. The LMWH/P N/MPs-coated ...

https://www.mdpi.com/1422-0067/16/5/11785/htm

Cell-derived Microparticles. Extracellular membrane vesicles generated by the shedding of CELL MEMBRANES blebs. Microparticles ... Membrane microparticles are submicron fragments of membrane vesicles shed from various cell types. Circulating endothelial ... Microparticles are used in a variety of different fields, such as drug delivery. Recently, non-spherical microparticle ... and other cell types circulate in the peripheral blood and through the MICROVASCULATURE where larger cells cannot, functioning ...

https://www.bioportfolio.com/resources/pmarticle/2282537/Synthesis-and-fabrication-of-surface-active-microparticles-using-membrane-emulsion-technique-and.html

CONCLUSION(S) Cell-derived microparticles were raised in normal pregnancy. Recurrent miscarriage seems to be related to ... endothelial and platelet cell activation and/or consumption. An increase in almost all cMP types was observed in PE. A ... OBJECTIVE To assess current studies on the relationship between cell-derived microparticles (cMP) and recurrent miscarriages ( ... Circulating cell-derived microparticles in severe preeclampsia and in fetal growth restriction.. Jaume Alijotas-Reig, Carles ...

https://www.semanticscholar.org/paper/Cell-derived-microparticles-and-vascular-pregnancy-Alijotas-Reig-Palacio-Garc%C3%ADa/1bc254244eb1daf1b0cf3daa86277f607f13f2f9

Circulating cell-derived microparticles in malaria patients Nantakomon D., Udomsangpetch R., Pattanapunyasat K., Looareesuwan S ...

https://www.tropicalmedicine.ox.ac.uk/publications/11042

Cell-Derived Microparticles Promote Coagulation after Moderate Exercise. SOSSDORF, MAIK; OTTO, GORDON P.; CLAUS, RALF A.; More ...

https://journals.lww.com/acsm-msse/toc/2011/07000

... monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T ... are excellent vehicles for releasing molecules inside the cell. The aim of this work was to use MSMPs to deliver viral specific ... MHC class I restricted epitopes into human antigen presenting cells ( ... The microparticles are internalized into dendritic cells (DC). (a) Photographs show control cells (MDDCs) or with internalized ...

https://www.hindawi.com/journals/jir/2013/362163/

... circulating levels of endothelial cell-derived microparticles and bone marrow-derived endothelial progenitor cells, and indices ... endothelial cell injury, as represented by elevated levels of circulating endothelial cell-derived microparticles (EMPs), and ... Endothelial cell-derived microparticles. EC injury is assessed by measuring circulating EMPs, which are markers of activated or ... Cellular biomarkers of endothelial health: microparticles, endothelial progenitor cells, and circulating endothelial cells. J ...

https://bmjopen.bmj.com/content/8/5/e020525

Improving the dissolution of fenofibrate with yeast cell-derived hollow core/shell carbon microparticles. In: RSC Advances. ... Improving the dissolution of fenofibrate with yeast cell-derived hollow core/shell carbon microparticles. RSC Advances. 2016 ... Improving the dissolution of fenofibrate with yeast cell-derived hollow core/shell carbon microparticles. / Huang, Xiaoxi; ... title = "Improving the dissolution of fenofibrate with yeast cell-derived hollow core/shell carbon microparticles", ...

https://sofi-northwestern.pure.elsevier.com/en/publications/improving-the-dissolution-of-fenofibrate-with-yeast-cell-derived-

Circulating Endothelium-Derived Microparticles (EMPs). Preparation of platelet-free plasma. Endothelium-derived microparticles ... endothelial microparticle. EPC. endothelial progenitor cell. FMD. flow-mediated dilation. L-NMMA. LG-monomethyl-arginine. NO. ... 2003) Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis. Thromb ... 2003) Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis. Thromb ...

http://www.onlinejacc.org/content/51/18/1760?ijkey=6db2ae98a9c90c14e7b2413054be4c9bac971864&keytype2=tf_ipsecsha

Neutrophil microparticles-induced injuries and morphological alterations in human umbilical vein endothelial cells (HUVECs) ... In this study we investigated the potential role of myeloperoxidase in the endothelial cell injury caused by neutrophil-derived ... Exposure of HUVEC to neutrophil microparticles induced a loss of cell membrane integrity and morphological changes. The ... in neutrophil microparticles and that the microparticle-associated myeloperoxidase cause injury to endothelial cells. Hence, ...

https://bmcmolcellbiol.biomedcentral.com/articles/10.1186/1471-2121-15-21

Levels of circulating EPC-microparticles (MPs) and endothelium-derived MPs (EMPs) in db/db mice are changed in basal and after ... A and B: the P1 gate for CD45+ cells. C-F: EPCs are defined as CD34+/VEGFR+ (top right) cells gated on P1 gate (C: db/+ sham; D ... Circulating endothelial progenitor cells and cellular membrane microparticles in db/db diabetic mouse: possible implications in ... Circulating endothelial progenitor cells and cellular membrane microparticles in db/db diabetic mouse: possible implications in ...

https://www.ncbi.nlm.nih.gov/pubmed/21505143?dopt=Abstract

Thus, microparticles can transfer chemokine receptors and arachidonic acid between cells, activate complement, promote ... A role in inflammatory arthritis is suggested by the finding that leukocyte-derived microparticles induce the production of ... Under certain conditions, however, microparticles may also exert anti-inflammatory properties by inducing immune cell apoptosis ... Microparticles may play an important role in the pathogenesis of rheumatologic diseases as evidenced by their elevation in ...

https://www.semanticscholar.org/paper/Microparticles-as-mediators-of-cellular-cross-talk-Distler-Huber/3b964c21748367d7a4367964a2f53e0add1ebc2b

Cell-cell communication has proven to be even more complex than previously thought since the discovery that extracellular ... Cell-derived microparticles. *Exosomes. *Microrna. Get free article suggestions today. Mendeley saves you time finding and ... Cell-cell communication has proven to be even more complex than previously thought since the discovery that extracellular ... More than simply cell dust, extracellular vesicles are capable of transferring biological information to neighboring cells and ...

https://www.mendeley.com/research-papers/microvesicles-cellcell-messengers-cardiovascular-diseases/

Cell-Derived Microparticles in Patients with Type 2 Diabetes Mellitus: a Systematic Review and Meta-Analysis ... Type 2 diabetes mellitus Microparticles Biomarker Platelet-derived microparticle Endothelium-derived microparticle ... Supplementary Material for: Cell-Derived Microparticles in Patients with Type 2 Diabetes Mellitus: a Systematic Review and Meta ... i,Background/Aims: ,/i,The aim of this study was to assess the association between circulating cell-derived microparticles (MPs ...

https://karger.figshare.com/articles/Supplementary_Material_for_Cell-Derived_Microparticles_in_Patients_with_Type_2_Diabetes_Mellitus_a_Systematic_Review_and_Meta-Analysis/4224207/1

Circulating cell-derived microparticles (MPs) are sub-micronic phospholipid vesicles derived from cells of different types in ... Cell-derived microparticles (MPs) and their role in unexplained recurrent pregnancy loss * Rucha Kiran Patil National Institute ... There are many studies which show the association of specific cell-derived MPs to different pathological states. MPs act as a ...

http://insajournal.in/insaojs/index.php/proceedings/article/view/473

PDMP, monocyte-derived microparticles (MDMP), and endothelial cell-derived microparticles (EDMP) were detected by flow cytometry using a modified version of the previously reported method. (nature.com)
Levels of four MPs in circulation, (1) platelet-derived activated MPs (PDAc-MPs), (2) platelet-derived apoptotic MPs (PDAp-MPs), (3) endothelial-derived activated MPs (EDAc-MPs), and (4) endothelial-derived apoptotic MPs (EDAp-MPs), were measured just after the patient was enrolled into the study using flow cytometry. (hindawi.com)
Microparticles were identified by flow cytometry. (nih.gov)
The EPCs (CD133 + /KDR + , CD34 + /KDR + ) and endothelial microparticles (EMPs: CD31 + /CD41 − , CD144 + , CD62e + ) were determined in blood using flow cytometry. (onlinejacc.org)
Microparticles were produced by activating human neutrophils with a calcium ionophore and characterized by flow cytometry and transmission and scanning electron microscopy. (biomedcentral.com)
Neutrophil microparticles-induced injuries and morphological alterations in human umbilical vein endothelial cells (HUVECs) were evaluated by microscopy and flow cytometry. (biomedcentral.com)
In this study, characterization of PMPs and cell lines were done by flow cytometry. (ac.ir)
RESEARCH DESIGN AND METHODS Levels and cellular origin of vitreous and plasma microparticles from control ( n = 26) and diabetic ( n = 104) patients were analyzed by flow cytometry, and their proangiogenic activity was assessed by in vitro thymidine incorporation and neovessel formation in subcutaneous Matrigel plugs in mice. (diabetesjournals.org)
By inducing ALI with intratracheal instillation of lipopolysaccharide (LPS) in C57BL6 mice, the authors found that MVs released into the injured alveolus were mainly from alveolar macrophages initially by flow cytometry, peaking at 1 hour and followed soon thereafter by MVs released by alveolar epithelial cells and neutrophils by 4 hours. (bmj.com)
Second, in early ALI following intratracheal LPS instillation, alveolar macrophages were the initial source of the pro-inflammatory MVs, followed soon thereafter by MVs released from alveolar epithelial cells and neutrophils, as determined by flow cytometry. (bmj.com)
Sarah E. Headland, Hefin R. Jones, Adelina S. V. D'Sa, Mauro Perretti & Lucy V. Norling Cutting-Edge Analysis of Extracellular Microparticles using ImageStreamX Imaging Flow Cytometry. (isac-net.org)
Analysis of antigen presenting cell derived exosomes, based on immuno‐magnetic isolation and flow cytometry. (currentprotocols.com)
AnnexinV-positive MP (TMP), MP derived from platelets (PMP), erythrocytes (ErMP), endothelial cells (EMP), leukocytes (LMP) and granulocytes (PNMP) were determined by flow cytometry. (diva-portal.org)
HUVECs (3 × 10 6 cells/condition) were treated with either high glucose-derived EMPs (hgEMPs) or normal glucose-derived (ngEMPs) for 24 h and surface expression of E-selectin (CD62E-PE), ICAM-1 (CD54-FITC), VCAM-1 (CD106-APC) and PECAM-1 (CD31-BV) was assessed by flow cytometry and reported as mean fluorescent intensity (MFI). (springermedizin.de)

This study tested the hypothesis that circulating microparticles (MPs) are useful biomarkers for predicting one-year mortality in patients with end-stage non-small cell lung cancer (ES-NSCLC). (hindawi.com)
INTRODUCTION: Circulating cell-derived microparticles (MPs) have been implicated in several disease processes and elevated levels are found in many pathological conditions. (uzh.ch)
Circulating endothelial microparticles have been proposed as markers of endothelial injur. (bioportfolio.com)
Increased circulating cell-derived microparticle count is associated with recurrent implantation failure after IVF and embryo transfer. (semanticscholar.org)
Circulating microparticles: a marker of procoagulant state in normal pregnancy and pregnancy complicated by preeclampsia or intrauterine growth restriction. (semanticscholar.org)
Endothelium-dependent vasodilation, circulating levels of endothelial cell-derived microparticles and bone marrow-derived endothelial progenitor cells, and indices of nitric oxide inhibition are assessed before and 3, 40, 70 and 100 min after negative emotion induction. (bmj.com)
In addition to functional measures (ie, flow-mediated dilation), this study is also assessing cellular measures of endothelial function including circulating endothelial cell-derived microparticles and endothelial progenitor cells. (bmj.com)
We studied the effects of acute SHS exposure on the number and function of endothelial progenitor cells, plasma vascular endothelial growth factor, circulating endothelial microparticles, and flow-mediated vasodilation at serial time points until 24 h after exposure. (onlinejacc.org)
Circulating endothelial progenitor cells and cellular membrane microparticles in db/db diabetic mouse: possible implications in cerebral ischemic d. (nih.gov)
For determining the implications of circulating endothelial progenitor cells (cEPCs) and cellular membrane microparticles (MPs) in diabetic stroke, levels of EPCs, EPC-MPs, and endothelium-derived MPs (EMPs) and their correlations with blood glucose concentration, cerebral microvascular density (cMVD), and ischemic damage were investigated in type 2 diabetic db/db and db/+ (wild-type control) mice. (nih.gov)
The aim of this study was to assess the association between circulating cell-derived microparticles (MPs) and type 2 diabetes mellitus (T2DM). (figshare.com)
Circulating cell-derived microparticles (MPs) are sub-micronic phospholipid vesicles derived from cells of different types in response to various biological processes such as cellular activation and apoptosis. (insajournal.in)
It has been known that levels of circulating cell-derived microparticles (MP) are generally increased in inflammatory states, making them useful as biomarkers. (ebrary.net)
In this paper, we describe how the circulating blood cells - monocytes, platelets, neutrophils and their microparticles - co-operate in regulating the expression, availability and activity of monocyte-derived TF. (biochemsoctrans.org)
Endothelial microparticles are small vesicles that are released from endothelial cells and can be found circulating in the blood. (wikipedia.org)
Although circulating endothelial microparticles can be found in the blood of normal individuals, increased numbers of circulating endothelial microparticles have been identified in individuals with certain diseases, including hypertension and cardiovascular disorders, and pre-eclampsia and various forms of vasculitis. (wikipedia.org)
and reducing circulating levels of bone marrow-derived endothelial progenitor cells (EPCs), a marker of EC reparative capacity (Hypothesis 1c). (clinicaltrials.gov)
Compared to the neutral condition, the depressed mood and separately the anxiety recall tasks will acutely impair endothelium-dependent arterial vasodilation, increase circulating levels of EMPs, and reduce circulating levels of bone marrow-derived EPCs. (clinicaltrials.gov)
Since the description of Trousseau syndrome in 1865, various experimental and clinical studies have detailed the interaction of platelets with primary tumors and circulating metastatic tumor cells. (ahajournals.org)
5,6 Taken together, these seminal observations suggest a robust interaction between circulating platelets and tumor cells. (ahajournals.org)
Circulating microparticles (MP) are involved in the pathogenesis of atherothrombotic disorders and are raised in individual with CVD. (diva-portal.org)
We analysed circulating (annexin V+) microparticles and their conjugates in resuscitated patients. (biomedcentral.com)
The endothelium is a monolayer of cells, called endothelial cells, that lines the interior of blood vessels, including arteries, veins and cardiac chambers, 1 acting as a protective layer between circulating blood and other tissues. (scielo.br)
1 The yield of endothelial cells culture from peripheral blood represents an important step in research on circulating angiogenic cells, since these cells were firstly isolated for research purpose. (scielo.br)
Prognostic value of circulating levels of stem cell growth factor beta (SCGF beta) in patients with Chagas' disease and idiopathic dilated cardiomyopathy. (genscript.com)

Mesenchymal stem or stromal cells (MSCs) are multipotent progenitor cells, which can be isolated from many tissues, such as bone marrow, adipose tissue, synovium or Wharton's jelly [ 1 ]. (thno.org)
Brief exposure to a real-world level of SHS not only causes acute vascular injury as indicated by endothelial dysfunction and microparticle generation, but also leads to sustained changes of the vascular repair system with a mobilization of dysfunctional endothelial progenitor cells. (onlinejacc.org)
Objectives This study sought to analyze the effects of acute secondhand smoke (SHS) exposure on the number and function of endothelial progenitor cells (EPCs) over 24 h. (onlinejacc.org)
EVs are secreted by all cell types including stem/progenitor cells. (springer.com)
In both human and animal models, the use of stem/progenitor cells (such as bone marrow mesenchymal stromal cells) has been shown to promote the recovery of kidney diseases such as acute kidney injury and chronic kidney disease. (springer.com)
Stem/progenitor cell-derived extracellular vesicles are an important mechanism by which stem/progenitor cells might repair kidney injury. (springer.com)
17 - 21 As an example, overexpressing stromal derived factor-1 (CXCL12), an important chemokine in cardiac development, protects the myocardium and sustains the repairing after myocardial infarction, 22 by promoting regeneration and angiogenesis through recruitment of progenitor cells. (ahajournals.org)
Characterization of mRNA and microRNA in human mast cell-derived exosomes and their transfer to other mast cells and blood CD34 progenitor cells. (ispub.com)
Perpetual epithelial renewal is facilitated by stem and progenitor cells that balance proliferation and differentiation signals to continuously replace terminally differentiated or dying cells. (jci.org)
The encoded protein is a secreted sulfated glycoprotein and functions as a growth factor for primitive hematopoietic progenitor cells. (genscript.com)

abstract = "Hollow core/shell carbon microparticles, denoted HCSC600, are synthesized from yeast cells by coating the cells with silica shells, and then pyrolyzing the silica-protected yeast cells at 600°C, and finally etching the silica shells off of the carbonized products. (elsevier.com)

Cosenza S, Toupet K, Maumus M, Luz-Crawford P, Blanc-Brude O, Jorgensen C, Noël D. Mesenchymal stem cells-derived exosomes are more immunosuppressive than microparticles in inflammatory arthritis. (thno.org)
This prompted us to compare the function of exosomes (Exos) and microparticles (MPs) isolated from MSCs and investigate their immunomodulatory function in arthritis. (thno.org)
Extracellular vesicles are classified into exosomes, microvesicles/microparticles, or apoptotic bodies, originating from different subcellular compartments. (mendeley.com)
Furthermore, extracellular vesicles from supernatants of H4B4*KLH-pulsed MoDC contained significantly more HLA-DR and KLH than those purified from control MoDC, and KLH was concentrated specifically in exosomes that were a uniquely effective source of Ag in standard T cell proliferation assays. (jimmunol.org)
In summary, we identify LAMP-2 as an endocytic receptor on human MoDC that routes cargo into unusual Ag processing pathways, which reduces surface expression of Ag-derived peptides while selectively enriching Ag within immunogenic exosomes. (jimmunol.org)
Once considered cellular debris or, more recently, as biomarkers of disease progression, extracellular vesicles (EVs), comprised of exosomes, microvesicles (MVs) and apoptotic bodies, released by living and dying eukaryotic cells are now recognised as important mediators of cellular communication and function. (bmj.com)
Exosomes are formed by invagination within a cell to create an intracellular vesicle called an endosome, or an endocytic vesicle. (wikipedia.org)
As a potential mechanism, Mackie et al found that CD34 Shh cells are able to deposit greater amount of Shh in exosomes and transfer them to other cell types, activating the Shh signaling pathway in recipient cells. (ahajournals.org)
Among all cell types, only CD34 + cells preferentially store Shh in exosomes. (ahajournals.org)
Likewise, it is not clear whether these exosomes or microparticles also transport other factors potentially modulating these processes. (ahajournals.org)
Exosomes are membrane vesicles of intracellular origin that are secreted from the cell by exocytosis. (isac-net.org)
Characterization and proteomic profiling of pancreatic cancer-derived serum exosomes. (abcam.com)
Exosomes harbor B cell targets in pancreatic adenocarcinoma and exert decoy function against complement-mediated cytotoxicity. (abcam.com)
Exosomes released by hepatocarcinoma cells endow adipocytes with tumor-promoting properties. (abcam.com)
Exosomes are nanoparticles (~100 nm diameter) released from cells, which can transfer small RNAs and mRNA via the extracellular environment to cells at distant sites. (izon.com)

Microvesicles (ectosomes, or microparticles) are a type of extracellular vesicle (EV) that are released from the cell membrane. (wikipedia.org)
In multicellular organisms, microvesicles and other EVs are found both in tissues (in the interstitial space between cells) and in many types of body fluids. (wikipedia.org)
Microvesicles play a role in intercellular communication and can transport molecules such as mRNA, miRNA, and proteins between cells. (wikipedia.org)
Though initially dismissed as cellular debris, microvesicles may reflect the antigenic content of the cell of origin and have a role in cell signaling. (wikipedia.org)
Microvesicles may also remove misfolded proteins, cytotoxic agents and metabolic waste from the cell. (wikipedia.org)
Different cells can release microvesicles from the plasma membrane. (wikipedia.org)
Microvesicles are small, plasma membrane-derived particles that are released into the extracellular environment by the outward budding and fission of the plasma membrane. (wikipedia.org)
Ectosomes, also commonly referred to as microvesicles or microparticles, are shed from the plasma membrane. (isac-net.org)
Several different measurement approaches can be taken for the analysis of cell-derived microvesicles, and each has their advantages and disadvantages. (isac-net.org)
Because of the potential importance of microvesicles as diagnostic or prognostic markers, there is significant interest in standardizing sample preparation and measurement approaches for cell-derived microvesicles. (isac-net.org)
To investigate this mechanism, we focused on tumor-derived microvesicles (TMV). (plos.org)
Recent studies report that crosstalk between tumor cells and stromal cells are important for tumor progression or acceleration of tumor malignancy by secreting microvesicles (MVs) [11] , [12] . (plos.org)
After activation, platelets release small vesicles, called microparticles or microvesicles. (ahajournals.org)
Microvesicles (MVs) are cell-derived vesicles which are of interest in a clinical setting, as they may be predictive of early signs of disease and/or of treatment progression. (izon.com)
Stable cell fate changes in marrow cellsinduced by lung-derived microvesicles. (ispub.com)

In this study, we evaluated the effects on matrix catabolic activity of AF cells by the extracellular endothelial microparticles (EMPs) and soluble protein factors (SUP fraction) produced from ECs. (elsevier.com)
Passage 1 human AF cells grown in monolayer cultures were treated for 72 h with 250 µg of EMPs or SUP fraction isolated from culture of the microvascular endothelial cell line, HEMC-I. Live-cell imaging revealed uptake of EMPs by AF cells. (elsevier.com)
RT-PCR analysis demonstrated increased mRNA expression of MMP-1 (50.3-fold), MMP-3 (4.5-fold) and MMP-13 (5.5-fold) in AF cell cultures treated with EMPs compared to untreated control. (elsevier.com)
Increased MMP expression is primarily due to EMP or SUP stimulation of AF cells since EMPs or SUP fraction alone contained negligible amount of MMPs. (elsevier.com)
Interestingly, MMP activity was elevated in AF cell cultures treated with EMPs but not with SUP. (elsevier.com)
This study revealed enhanced matrix catabolism as a molecular consequence of action of ECs on AF cells via EMPs, which might be expected during neo-angiogenesis of degenerating disc. (elsevier.com)
Two blood samples were drawn, one immediately and one 24 hours after return of spontaneous circulation (ROSC) to detect (annexin V+) monocyte-derived microparticles (MMPs) or procoagulant (annexin V+) platelet-derived microparticles (PMPs) and conjugates of endothelial-derived (annexin V+) microparticles (EMPs) with monocytes (EMP-MC) or platelets (EMP-PC). (biomedcentral.com)
The experimental aim of this study was to determine, in vitro, the effects of glucose-induced EMPs on endothelial cell expression of E-selectin, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 and platelet cell adhesion molecule-1 (PECAM-1). (springermedizin.de)
Hyperglycemic-derived EMPs induced significantly higher surface expression of E-selectin (2614 ± 132 vs. 2010 ± 204 MFI), ICAM-1 (2110 ± 81 vs. 1688 ± 152 MFI), VCAM-1 (3589 ± 431 vs. 2134 ± 386) and PECAM-1 (4237 ± 395 vs. 2525 ± 269 MFI) on endothelial cells than EMPs from normoglycemic conditions. (springermedizin.de)

In contrast, diagnosing vascular complications in patients undergoing SCT is challenging, and damage to endothelial cells is regarded as the common feature of these complications. (nature.com)

7 , 8 In addition, these microparticles have more recently been shown to reflect in vitro cell stimulation, and testify to cellular activation and/or tissue degeneration occurring in vivo under a variety of pathophysiologic circumstances. (nature.com)
This study aimed to investigate whether KMSC-derived MPs show anti-fibrotic effects by ameliorating endothelial-to-mesenchymal transition (EndoMT) in human umbilical vein endothelial cells (HUVEC) in vitro and by preserving PTC in kidneys with unilateral ureteral obstruction (UUO) in vivo . (biomedcentral.com)
MPs isolated from the supernatants of KMSC were co-cultured with HUVEC to assess their in vitro biologic effects on endothelial cells. (biomedcentral.com)
KMSC-derived MPs significantly ameliorated EndoMT and improved in vitro proliferation of TGF-β1 treated HUVEC. (biomedcentral.com)
Detection of fetal DNA and RNA in placenta-derived syncytiotrophoblast microparticles generated in vitro. (semanticscholar.org)
EPC generation and functions were evaluated by in vitro cell cultures. (nih.gov)
The in vitro treatment of tumor cells with platelet [Plt] causes inhibition of tumor cell growth , although mechanism of this effect is not clear yet. (bvsalud.org)
Vaccines composed of DCs pulsed with the supernatants of chemically stressed CRC cells induced a more significant IFN-γ-producing Th1 response both in vitro and in vivo . (nature.com)
Through experiments performed in vivo and in vitro , DAMPs induced by chemical drugs have been demonstrated to stimulate dendritic cells (DCs) to induce an anti-cancer immune response. (nature.com)
Using IBD clinical samples, and in vitro and in vivo injury models, we show that PMN-derived miR-23a and miR-155 promote accumulation of double-strand breaks (DSBs) by inducing lamin B1-dependent replication fork collapse and inhibition of homologous recombination (HR) by targeting HR-regulator RAD51. (jci.org)
4 Tumor cells that aggregate platelets in vitro produce more lung metastases than tumor cells lacking such ability, illustrating the platelet-activating potential of some tumor cells. (ahajournals.org)
In vitro, the release of microparticles (MPs) from platelets [platelet-derived MPs (PMPs)] has been associated with the secretion response ( 4 ). (aaccjnls.org)
Inflammation determines the pro-adhesive properties of high extracellular d -glucose in human endothelial cells in vitro and rat microvessels in vivo. (springermedizin.de)
2 The methods include in vitro analysis, such as culture of endothelial cells, and in vivo analysis, such as flow-mediated dilation (FMD), venous occlusion plethysmography (VOP) and measurement of serum markers. (scielo.br)
Culture of endothelial cells has been widely described in the literature, especially in studies on the effect of oxidative stress and inflammation on mobilization and proliferation of these cells in vitro . (scielo.br)

The ability of microparticles to support coagulation was determined in normal plasma. (nih.gov)
Shimizu, M. Microparticles as biomarkers of blood coagulation in cancer. (eurekaselect.com)
Interactions of platelets, coagulation, and tumor cells in tumorigenesis. (ahajournals.org)
Schematic diagram showing the interplay among various proteins: platelet receptors, coagulation proteins, and tumor cells interacting in the process of tumorigenesis. (ahajournals.org)

Here, we investigate the role of microparticles (MP), a member of the extracellular vesicle family, in the exchange of pro-inflammatory molecules between brain immune cells, as well as their transfer to the systemic circulation, as key pathways of inflammation propagation following brain trauma. (biomedcentral.com)

PDMPs have been associated with TTP, and endothelial cells and monocytes can develop the same microparticles. (nature.com)
Methods:Human umbilical vein endothelial cells and U937 monocytes were stimulated with TNF-α to produce MPs. (le.ac.uk)
2 Injuries to the endothelium trigger an inflammatory response with participation of several cell types - lymphocytes, monocytes, platelets and smooth muscle cells 5 - culminating in endothelial cell dysfunction, stiffness of vessel wall and atherosclerotic plaque formation. (scielo.br)
In response to damage, epithelial wounds repair by a series of events that integrate epithelial responses with those of resident and infiltrating immune cells including neutrophils and monocytes/macrophages. (jci.org)

Synovial microparticles bound less annexin V (which binds to negatively charged phospholipids) than did plasma microparticles, exposed tissue factor, and supported thrombin generation via factor VII. (nih.gov)
Induction of plasma (TRAIL), TNFR-2, Fas ligand, and plasma microparticles after human immunodeficiency virus type 1 (HIV-1) transmission: implications for HIV-1 vaccine design. (semanticscholar.org)

In PDR, the endothelial microparticles ratio-but not that for platelet-was greater than 1.0, indicating local formation of endothelial microparticles from retinal vessels and permeation of platelet microparticles from plasma. (diabetesjournals.org)
23 , 24 Notably, the vasoregenerative and angiogenic potential of angiogenic early outgrowth cells can be enhanced by the transfer of the CXCL12 receptor CXCR4 through platelet microparticles. (ahajournals.org)
Platelet microparticles are small in size (0.05 to 1 μm) with a defined plasma membrane and express selected platelet membrane and cellular proteins. (ahajournals.org)
There is increasing research on and clinical interest in the physiological role played by platelet microparticles (PMPs). (izon.com)

Mesenchymal stem cells (MSCs) release extracellular vesicles (EVs) that display a therapeutic effect in inflammatory disease models. (thno.org)
Cell-cell communication has proven to be even more complex than previously thought since the discovery that extracellular vesicles serve as containers of biological information on various pathophysiological settings. (mendeley.com)
More than simply cell dust, extracellular vesicles are capable of transferring biological information to neighboring cells and play an active role in inflammatory diseases, including atherosclerosis and angiogenesis. (mendeley.com)
Here, this review will discuss the latest advances concerning the application potential of stem/progenitor cell-derived extracellular vesicles in renal diseases, including the aspects as follows: anti-inflammatory, proliferation-promoting and anti-apoptotic, proangiogenic, antifibrotic and renal cancer progression-promoting. (springer.com)
Therefore, stem/progenitor cell-derived extracellular vesicles may be a promising treatment tool for renal diseases. (springer.com)
Extracellular vesicles (EVs) are a heterogeneous class of membrane-bound cell-derived structures that can engage in intercellular communication via the trafficking of bioactive molecules between cells and tissues. (mdpi.com)
This review outlines the mechanisms and functions of dying-cell-derived EVs and their ability to drive inflammation during various modes of cell death, whilst reflecting on the challenges and knowledge gaps in investigating this subgenre of extracellular vesicles research. (mdpi.com)
Small Extracellular Vesicles in Rat Serum Contain Astrocyte-Derived Protein Biomarkers of Repetitive Stress. (abcam.com)
Priming of dendritic cells by DNA-containing extracellular vesicles from activated T cells through antigen-driven contacts. (abcam.com)
Extracellular vesicles (EVs) are the collective term for the various vesicles that are released by cells into the extracellular space. (izon.com)
Urinary extracellular vesicles (uEVs) are released by cells throughout the nephron and contain biomolecules from their cells of origin. (izon.com)
In this proof-of-principle study a label-free optical sensor is demonstrated to monitor the surface adhesion of extracellular vesicles secreted by live cells on to various extracellular matrix proteins. (izon.com)

Low molecular weight heparin (LMWH)/protamine (P) nano/micro particles (N/MPs) (LMWH/P N/MPs) were applied as carriers for heparin-binding growth factors (GFs) and for adhesive cells including adipose-derived stromal cells (ADSCs) and bone marrow-derived mesenchymal stem cells (BMSCs). (mdpi.com)
4 - 10 Among all, autologous bone marrow-derived CD34 + stem cells demonstrate a major benefit, showing favorable impact on angiogenic growth, without the toxicity and inflammatory response observed in nonautologous cell-based therapy. (ahajournals.org)

Washing samples results in decreased AnnV+MPs (P=0.002) and platelet-derived MPs (PMPs) (P=0.002). (uzh.ch)
Membrane-derived microparticles (PMPs) are produced from platelets during activation, storage, and apoptosis. (ac.ir)
In this study, the ability of PMPs to deliver CXCR4 molecule to CXCR4-null targets (Daudi, K562 and U937 cell line) was evaluated and the different concentrations of PMPs were examined to transfer CXCR4. (ac.ir)
PMPs in 7 different concentrations were added to cell culture plates and incubated for 1 hour at 37ºc and 5% CO2. (ac.ir)
PMPs in different concentrations can transfer CXCR4 to target cells. (ac.ir)
Also, the increase of PMPs concentration up to 250µg/mL can increase the CXCR4 presence on null cells. (ac.ir)
Platelet-derived microparticles (PMPs) are generally considered a marker of platelet activation in cardiovascular disease. (aaccjnls.org)

Microparticles (MPs) are small vesicles, which typically range in size from 0.1 to 1.5 μm [ 9 ], shed from the plasma membrane into the extracellular space by most eukaryotic cells undergoing activation or apoptosis. (biomedcentral.com)

The microparticles also expressed CD66b, CD62L and myeloperoxidase, which are all commonly expressed on the surface of neutrophils, as well as exposition of phosphatidylserine. (biomedcentral.com)
When instilled intratracheally in naive mice, MVs isolated from macrophages primed with LPS induced ALI with an increase in influx of neutrophils and cytokines/chemokines (eg, KC), in lung protein permeability and in the expression of ICAM-1 on alveolar epithelial type I and II cells (Figure 8). (bmj.com)
In one embodiment, the invention relates to a particle or cell separation device that separates specific cell types such as neutrophils or stem cells or particles from bodily fluids such as platelets and other blood products such as red cells and plasma. (google.com)
Specifically, the neutrophils (a type of white blood cell also known as polymorphonuclear cells) contain a variety of powerful enzymes that can cause tissue inflammation. (google.com)
outline a mechanism whereby neutrophils control epithelial fitness and genomic instability via delivery of miR-23a-and miR-155-containing microparticles. (jci.org)
As the first immune cell recruited to sites of inflammation, neutrophils and their cargo are crucial for shaping mucosal responses to infection, injury, and repair. (jci.org)
Fcγ receptor mediated phagocytosis includes formation of protrusions of the cell called a 'phagocytic cup' and activates an oxidative burst in neutrophils. (wikipedia.org)

However, the supernatants of chemically stressed CRC cells failed to induce phenotypic maturation and cytokine production in TLR4-deficient DCs, indicating an essential role of TLR4 in DAMP-induced DC maturation and activation. (nature.com)
Furthermore, pulsing with the supernatants of chemically stressed CRC cells did not efficiently induce an IFN-γ-producing Th1 response in TLR4-deficient DCs. (nature.com)
Moreover, the Shh exosomal transfer is able to induce Shh specific signaling not only in endothelial cells, but also in fibroblasts. (ahajournals.org)
Leicester Research Archive: Monocyte- and Endothelial-Derived Microparticles Induce an Inflammatory Phenotype in Human Podocytes. (le.ac.uk)
Monocyte- and Endothelial-Derived Microparticles Induce an Inflammatory Phenotype in Human Podocytes. (le.ac.uk)

Microparticles (MPs), small membrane fragments shed from various cell types, have been implicated in thrombosis, inflammation, and endothelial dysfunction. (thejns.org)
Those subtypes closely associated with thrombosis and endothelial dysfunction, for example, CD145+(TF)-associated MPs and CD146+(endothelial cell)-associated MPs, had the most durable response and demonstrated a significant negative correlation with radiographic infarction at 14 days after SAH. (thejns.org)
The endothelial microparticles in some of these disease states have been shown to have arrays of cell surface molecules reflecting a state of endothelial dysfunction. (wikipedia.org)
At the beginning of atherosclerotic plaque formation, endothelial dysfunction is characterized by increased expression and release of adhesion molecules, including endothelial selectin (E-selectin), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). (scielo.br)

Induction of apoptosis has been proposed as a mechanism of Plt effects on tumor cells . (bvsalud.org)
however, the role of DAMPs released from chemical drug-treated tumor cells in the activation of the immune response needs to be further elucidated. (nature.com)
Efficiently killing tumor cells with chemical drugs while enhancing induction of the anti-tumor immune response is becoming increasingly important. (nature.com)
Here we showed that TMV isolated from tumor cells were taken up by NEC through endocytosis. (plos.org)
Tumor blood vessels provide nutrition and oxygen, promote tumor progression, and serve as gatekeepers for tumor cells to metastasize to other organs. (plos.org)
MVs are released from various tumor cells and some progenitors of differentiated cells [14] . (plos.org)
Observations have suggested that platelets not only augment the growth of primary tumors via angiogenesis but endow tumor cells physical and mechanical support to evade the immune system and extravasate to secondary organs, the basis of metastatic disease. (ahajournals.org)
3 Others have shown that intravenous injection of some tumor cells may cause significant thrombocytopenia (50% to 70%) in mice. (ahajournals.org)
We found that myofibroblasts triggered cues in the tumor microenvironment, which led to induction of CXCL4L1 in tumor cells. (aacrjournals.org)
Human tumor cells stained for DAPI (blue), cytochrome c (red), and CHCHD4 (green). (jci.org)

The aim of this work was to use MSMPs to deliver viral specific MHC class I restricted epitopes into human antigen presenting cells (monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T lymphocytes. (hindawi.com)
The most powerful antigen presenting cells are dendritic cells (DCs), which bridge innate and adaptive immunity and are capable of initiating a primary immune response by activating naïve T cells [ 10 ]. (hindawi.com)
In this study we identify LAMP-1 (CD107a) and LAMP-2 (CD107b) on the surface of human monocyte-derived dendritic cells (MoDC) and show only LAMP-2 is internalized after ligation by specific Abs, including H4B4, and traffics rapidly but transiently to the MHC class II loading compartment, as does Ag conjugated to H4B4. (jimmunol.org)
One of the major challenges towards increasing the vaccine efficacy has been to develop maturation procedures that produce highly immunogenic dendritic cells that also demonstrate strong migratory skills. (dtu.dk)
To test the migratory skills of the dendritic cells induced by the different procedures we present a versatile and easy to use chip integrated migration platform. (dtu.dk)
Dendritic cells seeded into the polymer chip in a concentration gradient of the chemoattractant CCL21 efficiently negotiated the microporous maze structure for pore sizes of 8x8 μm or larger. (dtu.dk)
Linear microchannels with diameters from 10 μm to 20 μm were also produced and simultaneous observations of dendritic cells migrating in the confined channels and in the fibrillar collagen were performed. (dtu.dk)
To more precisely mimic the mechanical and chemical properties of the tissue traversed by the dendritic cells we also present a poly (ethylene glycol) diacrylate (PEGDA) based strategy to fabricate soft 3D hydrogel scaffolds. (dtu.dk)
Dendritic cells also reside in tissues and ingest pathogens by phagocytosis. (wikipedia.org)

We measured and compared the levels of microparticles and soluble factors in patients undergoing allogeneic SCT. (nature.com)
Table 1 shows changes in the soluble factors and microparticles. (nature.com)
By mimicking pathogen dimensions, microparticles are more prone to be phagocyted by APCs than soluble antigen. (hindawi.com)
Because of alternative splicing TF is expressed in 3 mRNA splice variants: full-length (fl)TF, alternatively spliced (as)TF, and a third variant named TF-A. Translation of flTF and asTF mRNA splice variants leads to the generation of the flTF isoform, a membrane-bound and highly procoagulant protein (8), and soluble asTF with low prothrombogenic potential but strong proangiogenic, cell proliferation-facilitating, and prosurvival activities (2, 9, 10). (thefreelibrary.com)

Cellular microparticles are fragments that shed almost spontaneously from the plasma membrane blebs of virtually all cell types when submitted to a number of stress conditions. (nature.com)
7 , 8 Therefore, there is a possibility that cellular microparticles exhibit the dynamic change after SCT. (nature.com)
To determine the cellular origin of synovial microparticles, their procoagulant properties, and their relationship to local hypercoagulation. (nih.gov)
Microparticles as regulators of inflammation: novel players of cellular crosstalk in the rheumatic diseases. (semanticscholar.org)
Microparticles as mediators of cellular cross-talk in inflammatory disease. (semanticscholar.org)
Shedding of microparticles by myofibroblasts as mediator of cellular cross-talk during normal wound healing. (semanticscholar.org)
Microparticles have been identified not only in human plasma but also in other tissues with high cellular activation, inflammation, or apoptosis, such as human atherosclerotic plaques or synovial fluid in rheumatoid arthritis ( 13 , 14 ). (diabetesjournals.org)
Microvesicle budding takes place at unique locations on the cell membrane that are enriched with specific lipids and proteins reflecting their cellular origin. (wikipedia.org)
1 , 2 Therefore, in the last decade, the efforts were focused on studying the complex molecular and cellular mechanisms contributing to improved myocardial remodeling and function after stem cell transplantation. (ahajournals.org)
Communication between dying cells and their environment is a critical process that promotes tissue homeostasis during normal cellular turnover, whilst during disease settings, it can contribute to inflammation through the release of intracellular factors. (mdpi.com)
Although once seen as inert cellular debris, a plethora of literature spanning numerous fields of medicine now describes EV-mediated cargo transfer that can directly modulate the functions of target cells and tissues [ 7 , 8 , 9 , 10 ]. (mdpi.com)
This emerging field pertains to nano & microparticles that carry proteins and RNA, both of which serve important cellular functions, and is likely to be an important player in the fast evolving field of nanomedicine. (ispub.com)
Although only a simple monolayer, the healthy endothelium is optimally placed and is able to respond to physical and chemical signals by production of a wide range of factors that regulate vascular tone, cellular adhesion, thromboresistance, smooth muscle cell proliferation, and vessel wall inflammation. (ahajournals.org)

Targeted inhibition of miR-23a and miR-155 in cultured intestinal epithelial cells and in acutely injured mucosa decreased the detrimental effects of PMNs and enhanced tissue healing responses, suggesting that this approach can be used in therapies aimed at resolution of inflammation, in wound healing, and potentially to prevent neoplasia. (jci.org)
Rapid self-renewal also supports epithelial cells' essential role in barrier regulation and wound repair. (jci.org)

Microparticles (MPs) derived from kidney-derived mesenchymal stem cells (KMSCs) have recently been reported to ameliorate rarefaction of peritubular capillaries (PTC) in ischemic kidneys via delivery of proangiogenic effectors. (biomedcentral.com)
Recently, myofibroblasts have been shown to rise from endothelial cells via endothelial-to-mesenchymal transition (EndoMT) induced by the transforming growth factor-β (TGF-β) family of regulatory polypeptides in experimentally induced fibrotic diseases. (biomedcentral.com)
The successful development of an advanced therapy medicinal product (ATMP) made of mesenchymal stem cells (MSCs) for the treatment of articular cartilage based on actual EU regulations and clinical recommendations lie within the realm. (selectbiosciences.com)
3,4 They were demonstrated to be the active component of mesenchymal stem cell therapy and thus have wide potential therapeutic uses. (ispub.com)

The administration of G-CSF can affect serum cytokine levels of these patients, 3 resulting in the generation of procoagulant factors such as platelet-derived microparticles (PDMP). (nature.com)

Sterile inflammation of endothelial cell-derived apoptotic bodies is mediated by interleukin-1α. (semanticscholar.org)
Numerous studies now indicate that microparticles have biological activities and may be involved in thrombosis, cell inflammation, angiogenesis and cell-to-cell communication ( 5 - 12 ). (diabetesjournals.org)
Avenanthramides attenuate inflammation and atrophy in muscle cells. (abcam.com)
Resuscitated patients show substantially increased levels of different (annexin V+) microparticles and their conjugates immediately and 24 hours after cardiopulmonary resuscitation, suggesting an early onset of inflammation, an ongoing endothelial activation and a procoagulatory state. (biomedcentral.com)
The authors provide extensive updates on the latest findings concerning the mechanisms of inflammation and introduce such new concepts and methodologies as "endothelial and leukocyte microparticles" and "gene microarray technology" to help explain important links between the central nervous system (CNS) and general inflammatory processes. (springer.com)

Microparticles are a heterogeneous population of membrane-coated vesicles which can be released from virtually all cell types during activation or apoptosis. (semanticscholar.org)
Microparticles are submicron membrane vesicles with biological activities, released following cell activation or apoptosis. (diabetesjournals.org)
Additionally, microparticles of CPR patients may contribute to endothelial apoptosis. (biomedcentral.com)

Shedding of membrane microparticles is a physiological process that accompanies cell growth and activation and that is enhanced by cytokines, reactive oxygen species, activation of apoptotic pathways, or increases in intracellular calcium leading to cytoskeleton reorganization. (diabetesjournals.org)

It involves virtually all renal intrinsic and infiltrating cells and is characterized by alterations in their phenotype and accumulation of excessive extracellular matrix proteins [ 1 - 4 ]. (biomedcentral.com)
The encapsulation of recombinant proteins in biocompatible and biodegradable nano- and microparticles is emerging as a promising approach to boost their immunogenicity by passively targeting them to antigen presenting cells (APCs) [ 7 - 9 ]. (hindawi.com)
Upon activation neutrophil releases microparticles - small plasma membrane vesicles that contain cell surface proteins and cytoplasmic matter, with biological activities. (biomedcentral.com)
Tumor-derived MVs (TMV) contain bioactive molecules such as microRNAs (miRNA), mRNAs, and/or proteins inside themselves [15] . (plos.org)
EVs are a heterogeneous class of membrane-bound cell-derived structures produced by all eukaryotic cells that can harbour bioactive molecules, including nucleic acids, lipids and proteins, derived from their parental cells [ 1 , 6 ]. (mdpi.com)

1 µm) and the expression of surface markers (CD66b for neutrophil-derived MPs and CD41b for platelet-derived MPs). (scielo.br)
In this study we investigated the potential role of myeloperoxidase in the endothelial cell injury caused by neutrophil-derived microparticles. (biomedcentral.com)
This mechanism of neutrophil-derived microRNA shuttling opens up new therapeutic potential to enhance epithelial healing and limit mucosal injury. (jci.org)

Membrane microparticles are submicron fragments of membrane vesicles shed from various cell types. (bioportfolio.com)
Microparticles are submicron membrane vesicles shed from the cell surface of both healthy and damaged cells ( 4 ). (diabetesjournals.org)

Plasma from patients and healthy controls contained comparable numbers of microparticles, which originated from platelets and erythrocytes. (nih.gov)

We identified what we believe is a new, ROS-independent mechanism whereby activated tissue-infiltrating PMNs release microparticles armed with proinflammatory microRNAs (miR-23a and miR-155). (jci.org)

Submicron cell-derived microparticles (MP) are important biological messengers. (ovid.com)

Therefore, we aimed to detect the presence neutrophil (CD66b+) and platelet (CD41b+) derived microparticles in gingival crevicular fluid from individuals having periodontitis aggravated by type 2 diabetes. (scielo.br)
In conclusion, this study reported the presence of neutrophil and platelet derived microparticles in gingival crevicular fluid from individuals having severe periodontitis and type 2 diabetes. (scielo.br)

Microparticle content of platelet concentrates is predicted by donor microparticles and is altered by production methods and stress. (semanticscholar.org)
The microparticles are characterized by various methods, including scanning electron microscopy, transmission electron microscopy, nitrogen gas adsorption/desorption and X-ray photoelectron spectroscopy. (elsevier.com)
However, they also highlight the need for new, more sensitive methods for measuring cell-derived vesicles. (isac-net.org)

The lysosome-associated membrane protein (LAMP) family includes the dendritic cell endocytic receptors DC-LAMP and CD68, as well as LAMP-1 and LAMP-2. (jimmunol.org)
The membrane of the endothelial microparticle contains receptors and other cell surface molecules which enable the identification of the endothelial origin of the microparticle, and allow it to be distinguished from microparticles from other cells, such as platelets. (wikipedia.org)
Engaging multiple receptors with bispecific biologics offers the potential to improve upon single-agent checkpoint blockade and promises to be the next generation of immunotherapy with preclinical, translational and clinical studies on using bispecific antibodies for dual blockade of checkpoint targets, T-cell-redirecting bispecific biologics, overcoming T-cell exhaustion, as well as strategies to improve efficacy and reduce toxicity, and engineer the next generation of bi- and multi-specific biologics. (genomicglossaries.com)
Among these are receptors that recognise the Fc part of bound IgG antibodies, deposited complement or receptors, that recognise other opsonins of cell or plasma origin. (wikipedia.org)
Monocyte-derived microparticles (MMPs) contain organized membrane receptors including ß2-integrins, like Mac-1 (CD11b/CD18). (biomedcentral.com)

We tested the hypothesis that proangiogenic microparticles accumulate in vitreous fluid in diabetic retinopathy. (diabetesjournals.org)

Exos and MPs from MSCs inhibited T lymphocyte proliferation in a dose-dependent manner and decreased the percentage of CD4 + and CD8 + T cell subsets. (thno.org)
One major role of MSCs is to suppress proliferation and function of cells of both innate and adaptive immunity [ 2 ]. (thno.org)
Furthermore, three-dimensional (3D) cultures of cells including ADSCs and BMSCs using plasma-medium gel with LMWH/P N/MPs exhibited efficient cell proliferation. (mdpi.com)
Isolated vitreous microparticles stimulated by 1.6-fold endothelial proliferation and increased new vessel formation in mice. (diabetesjournals.org)
KLH) and recall (Bet v 1) Ags (H4B4*KLH and H4B4*Bet v 1) induced significantly less CD4 cell proliferation than pulsing with native Ag or Ag conjugated to control mAb (ISO*KLH and ISO*Bet v 1). (jimmunol.org)
Paradoxically, MoDC pulsed with H4B4*KLH, but not the other KLH preparations, induced robust proliferation of CD4 cells separated from them by a transwell membrane, indicating factors in the supernatant were responsible. (jimmunol.org)
CXCL4L1 has been shown to be more potent than CXCL4 at inhibiting cell proliferation and migration ( 2, 3 ). (aacrjournals.org)

In contrast, FIXa, which is also generated by the TF-FVIIa complex in isolation or on TF-bearing cells, is more stable compared with FXa, allowing time for transport from TF-bearing cells to e.g. activated platelets, without significant inactivation by plasma inhibitors. (biochemsoctrans.org)
Rapid isolation of two distinct plasma membrane microparticle populations for biomarker discovery and assay. (ispub.com)

PMP can transfer some adhesion molecules such as CXCR4 to CXCR4-negative cells. (ac.ir)
CXCR4-negative cell lines (1×105 cells/ml) were cultured in RPMI1640 with 10% FBS and 1% antibiotic. (ac.ir)
The presence of CXCR4 on cells was analyzed by flowcytometry. (ac.ir)

The CD142+(tissue factor [TF])-associated MPs and CD146+(endothelial cell)-associated MPs were significantly elevated throughout the study period. (thejns.org)

Although MSCs can prevent arthritis, the role of MSCs-derived EVs has never been reported in rheumatoid arthritis. (thno.org)
Therefore, endothelial microparticles may be useful as an indicator or index of the functional state of the endothelium in disease, and may potentially play key roles in the pathogenesis of certain diseases, including rheumatoid arthritis. (wikipedia.org)

Cell-derived microparticles (MPs) have been described as vital contributors to the inflammatory process. (scielo.br)
Both MSCs-derived MPs and Exos exerted an anti-inflammatory role on T and B lymphocytes independently of MSCs priming. (thno.org)
Our work is the first demonstration of the therapeutic potential of MSCs-derived EVs in inflammatory arthritis. (thno.org)
Renal tubulointerstitial damage and inflammatory cell infiltration were examined with Masson's trichrome, F4/80 and α-smooth muscle actin (α-SMA) staining and PTC rarefaction index was determined by CD31 staining. (biomedcentral.com)
Furthermore, administration of KMSC-derived MPs inhibited inflammatory cell infiltration as well as tubulointerstitial fibrosis in UUO mice as demonstrated by decreased F4/80 and α-SMA-positive cells and Masson's trichrome staining, respectively. (biomedcentral.com)
2) the effects of SCA/AA-RBC-MPs on human aortic endothelial cell (HAEC) inflammatory phenotype and TLR4 pathway. (frontiersin.org)
OBJECTIVE To assess current studies on the relationship between cell-derived microparticles (cMP) and recurrent miscarriages (RM) and pre-eclampsia (PE), and review the relationships between cMP and inflammatory and clot pathways, antiphospholipid antibodies (aPL), cytokines, and pregnancy complications. (semanticscholar.org)
Due to their ability to independently initiate inflammatory responses, MP derived from activated microglia may provide a potential therapeutic target for other neurological disorders in which neuroinflammation may be a contributing factor. (biomedcentral.com)
4-6 In the current issue of Thorax , Soni et al 7 studied the temporal pattern of EV release in early ALI and found that MVs from alveolar macrophages were the predominant source and played a significant role as potent initiators of the inflammatory response in part by the transfer of its cargo, the cytokine tumour necrosis factor (TNF), to target cells. (bmj.com)
In experiments to study the mechanisms of MVs, the authors found that MVs released by primary cultures of mouse macrophages primed with LPS to obtain a pro-inflammatory phenotype also induced ICAM-1 expression in MLE-12 cells, which was dependent on the TNF cargo of the MVs. (bmj.com)
To fully understand the complex mechanisms mediating the recovery of the heart after an ischemic insult, one needs to consider not only effects on cells directly involved, but also collateral effects on the inflammatory reaction and the paracrine secretion of remote cells. (ahajournals.org)
In particular, during inflammatory settings such as acute tissue injury, infection and autoimmunity, the EV-mediated transfer of proinflammatory cargo from dying cells is an important process that can elicit profound proinflammatory effects in recipient cells and tissues. (mdpi.com)
Improved understanding of crosstalk between epithelial and immune cells during wound repair is necessary for development of better pro-resolving strategies to treat debilitating complications of disorders ranging from inflammatory bowel disease to diabetes. (jci.org)

Stem/progenitor cell-derived EVs have been identified to exert immunomodulatory effects on target cells through transferring protein molecules as well as regulatory effects on the phenotype of target cells through fusion with the target cells membrane and/or through direct endocytosis by target cells to transfer nucleic acid substances (such as mRNA, miRNA) to the target cells. (springer.com)
8 The phenotype of the cells may determine the functional effects of the released MVs. (bmj.com)

Numerous diseases, recently reported to associate with elevated microvesicle/microparticle (MP) counts, have also long been known to be characterized by accelerated immune complex (IC) formation. (nih.gov)

Direct detection of activated platelets and platelet‐derived microparticles in humans. (currentprotocols.com)
The development of cell-based therapy for clinical studies raises the question whether the application of cell-based products to humans is safe. (selectbiosciences.com)
Prostacyclin, derived by the action of the cyclooxygenase system, is another endothelium-derived vasodilator that acts independently of NO. 11 Although it may contribute to some of the other regulatory roles of the endothelium, it appears to have a more limited role in the maintenance of vasodilator tone in humans. (ahajournals.org)

Human umbilical vein endothelial cells (HUVECs) were cultured (3rd passage) and plated in 6-well plates at a density of 5.0 × 10 5 cells/condition. (springermedizin.de)

Tiotropium inhibits proinflammatory microparticle generation by human bronchial and endothelial cells. (nih.gov)

Microparticles have recently emerged as a thrombotic risk marker with a potential role in determining which patients are at greatest risk for developing thrombosis. (bioportfolio.com)

CONCLUSIONS The present study demonstrates that vitreous fluid contains shed membrane microparticles of endothelial, platelet, and retinal origin. (diabetesjournals.org)

This hypothesis is based on the relatively rapid inactivation of the FXa formed on TF-bearing cells when it is released into the plasma. (biochemsoctrans.org)

Extracellular membrane vesicles generated by the shedding of CELL MEMBRANES blebs. (bioportfolio.com)

Leukocyte-derived MPs may also have a predictive role, although the studies regarding these are still limited. (eurekaselect.com)
There is an associated increase in platelet and leukocyte-derived microparticles. (bloodjournal.org)

Although stem cell transplantation (SCT) is being used for hematopoietic reconstitution following high-dose chemotherapy for malignancy, it involves some serious transplant-related complications. (nature.com)
11 , 12 However, insufficient number of donor stem cells, low cell viability, and inefficient expansion techniques limit the effects of this therapy. (ahajournals.org)
Because low viability and inefficient expansion are important limitations of stem-cell therapies, 1 enhancing stem-cell survival in the hostile environment is the main goal of current cell-based therapy. (ahajournals.org)
19 Genetic reprogramming of the transplanted stem cells allows them to serve as a source of growth factors, initiating intracrine, autocrine, and paracrine effects and augmenting the activity of endogenous cells. (ahajournals.org)
This dual approach, using stem-cell transplantation and genetic engineering to potentiate stem cell activity for myocardial repair, seems to be a reliable method and is currently successfully used in many experimental settings. (ahajournals.org)
Furthermore, EVs are increasingly recognised as potential diagnostic tools, serving as biomarkers for disease when isolated from biological fluids [ 11 , 12 , 13 , 14 ], whilst the therapeutic applications of stem-cell-derived EVs have demonstrated promising regenerative and immunomodulatory effects across a number of diseases [ 15 , 16 , 17 ]. (mdpi.com)
The presentation illustrates the legal gateways to the European market for (stem) cell based products from (basic) research to market approval. (selectbiosciences.com)
Experimental to clinical transplantation of pluripotent stem cells for cell therapy in retinal disease. (selectbiosciences.com)
The transformation of autologous adipose stem cells in a 3-dimensional osteogenic graft can open a new way for surgical reconstruction of large bone defect (after tumour resection and pseudarthrosis) with minimal invasive procedure. (selectbiosciences.com)
The articles in this dermatology Review Series demonstrate recent progress in understanding the cell biology and molecular pathophysiology of the epidermis and hair follicles, which harbor keratinocyte and melanocyte stem cells. (jci.org)

Bauer, N.N. On the origin of microparticles: From "platelet dust" to mediators of intercellular communication. (eurekaselect.com)
Star, R.A. Microparticles: markers and mediators of sepsis-induced microvascular dysfunction, immunosuppression, and AKI. (eurekaselect.com)

Anatomy17

Organisms1

Diseases5

Chemicals and Drugs63

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes13

Disciplines and Occupations6

Technology, Industry, Agriculture19

Health Care2

Tissue factor activity is increased in a combined platelet and microparticle sample from cancer patients. (1/383)

Microparticles are vectors of paradoxical information in vascular cells including the endothelium: role in health and diseases. (2/383)

The role of cell death in the pathogenesis of autoimmune disease: HMGB1 and microparticles as intercellular mediators of inflammation. (3/383)

The relationship between plasma microparticles and disease manifestations in patients with systemic sclerosis. (4/383)

Measurement of platelet-derived microparticle levels in the chronic phase of cerebral infarction using an enzyme-linked immunosorbent assay. (5/383)

Megakaryocyte-derived microparticles: direct visualization and distinction from platelet-derived microparticles. (6/383)

Dynamics of circulating microparticles in liver transplant patients. (7/383)

Diagnostic role of endothelial microparticles in vasculitis. (8/383)

Cell-Derived Microparticles

Particle Size

Microspheres

Polyglycolic Acid

Drug Compounding

Drug Carriers

Polyglactin 910

Blood Platelets

Chitosan

Delayed-Action Preparations

Lactic Acid

Drug Delivery Systems

Chemistry, Pharmaceutical

Polymers

Microscopy, Electron, Scanning

Nanoparticles

Cell Membrane Structures

Technology, Pharmaceutical

Capsules

Platelet Activation

Annexin A5

Thromboplastin

Blood Coagulation

Endothelial Cells

Powders

Flow Cytometry

Platelet Membrane Glycoprotein IIb

Polystyrenes

Silicon

Hexuronic Acids

Coagulants

Polyanhydrides

Glucuronic Acid

Surface Properties

Dosage Forms

Solubility

Polyesters

Polymethacrylic Acids

Excipients

Acrylates

Endothelium, Vascular

Powder Diffraction

Porosity

Calorimetry, Differential Scanning

Alginates

Platelet Glycoprotein GPIb-IX Complex

Hydrogels

Thrombosis

Lecithins

Magnetics

Biocompatible Materials

Emulsions

Aerosols

Antibodies, Immobilized

Hydrogel

Desiccation

Administration, Inhalation

Thrombin

Swiss 3T3 Cells

Phosphatidylserines

Thermogravimetry

P-Selectin

Polyethylene Glycols

Microfluidic Analytical Techniques

Spectroscopy, Fourier Transform Infrared

Electroplating

Miniaturization

Materials Testing

Antigens, CD31

Nanostructures

Carboxymethylcellulose Sodium

Vaccines

Methylene Chloride

Microfluidics

Gelatin

Dermatitis, Phototoxic

Nucleic Acids

Blood Preservation

Caproates

Methylcellulose