Cell-Derived Microparticles: Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.Particle Size: Relating to the size of solids.Microspheres: Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.Polyglycolic Acid: A biocompatible polymer used as a surgical suture material.Drug Compounding: The preparation, mixing, and assembling of a drug. (From Remington, The Science and Practice of Pharmacy, 19th ed, p1814)Drug Carriers: Forms to which substances are incorporated to improve the delivery and the effectiveness of drugs. Drug carriers are used in drug-delivery systems such as the controlled-release technology to prolong in vivo drug actions, decrease drug metabolism, and reduce drug toxicity. Carriers are also used in designs to increase the effectiveness of drug delivery to the target sites of pharmacological actions. Liposomes, albumin microspheres, soluble synthetic polymers, DNA complexes, protein-drug conjugates, and carrier erythrocytes among others have been employed as biodegradable drug carriers.Polyglactin 910: A polyester used for absorbable sutures & surgical mesh, especially in ophthalmic surgery. 2-Hydroxy-propanoic acid polymer with polymerized hydroxyacetic acid, which forms 3,6-dimethyl-1,4-dioxane-dione polymer with 1,4-dioxane-2,5-dione copolymer of molecular weight about 80,000 daltons.Blood Platelets: Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.Chitosan: Deacetylated CHITIN, a linear polysaccharide of deacetylated beta-1,4-D-glucosamine. It is used in HYDROGEL and to treat WOUNDS.Delayed-Action Preparations: Dosage forms of a drug that act over a period of time by controlled-release processes or technology.Lactic Acid: A normal intermediate in the fermentation (oxidation, metabolism) of sugar. The concentrated form is used internally to prevent gastrointestinal fermentation. (From Stedman, 26th ed)Drug Delivery Systems: Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.Chemistry, Pharmaceutical: Chemistry dealing with the composition and preparation of agents having PHARMACOLOGIC ACTIONS or diagnostic use.Polymers: Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).Microscopy, Electron, Scanning: Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.Nanoparticles: Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.Cell Membrane Structures: Structures which are part of the CELL MEMBRANE or have cell membrane as a major part of their structure.Technology, Pharmaceutical: The application of scientific knowledge or technology to pharmacy and the pharmaceutical industry. It includes methods, techniques, and instrumentation in the manufacture, preparation, compounding, dispensing, packaging, and storing of drugs and other preparations used in diagnostic and determinative procedures, and in the treatment of patients.Capsules: Hard or soft soluble containers used for the oral administration of medicine.Platelet Activation: A series of progressive, overlapping events, triggered by exposure of the PLATELETS to subendothelial tissue. These events include shape change, adhesiveness, aggregation, and release reactions. When carried through to completion, these events lead to the formation of a stable hemostatic plug.Annexin A5: A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.Thromboplastin: Constituent composed of protein and phospholipid that is widely distributed in many tissues. It serves as a cofactor with factor VIIa to activate factor X in the extrinsic pathway of blood coagulation.Blood Coagulation: The process of the interaction of BLOOD COAGULATION FACTORS that results in an insoluble FIBRIN clot.Endothelial Cells: Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.Powders: Substances made up of an aggregation of small particles, as that obtained by grinding or trituration of a solid drug. In pharmacy it is a form in which substances are administered. (From Dorland, 28th ed)Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Platelet Membrane Glycoprotein IIb: Platelet membrane glycoprotein IIb is an integrin alpha subunit that heterodimerizes with INTEGRIN BETA3 to form PLATELET GLYCOPROTEIN GPIIB-IIIA COMPLEX. It is synthesized as a single polypeptide chain which is then postranslationally cleaved and processed into two disulfide-linked subunits of approximately 18 and 110 kDa in size.Polystyrenes: Polymerized forms of styrene used as a biocompatible material, especially in dentistry. They are thermoplastic and are used as insulators, for injection molding and casting, as sheets, plates, rods, rigid forms and beads.Silicon: A trace element that constitutes about 27.6% of the earth's crust in the form of SILICON DIOXIDE. It does not occur free in nature. Silicon has the atomic symbol Si, atomic number 14, and atomic weight [28.084; 28.086].Hexuronic Acids: Term used to designate tetrahydroxy aldehydic acids obtained by oxidation of hexose sugars, i.e. glucuronic acid, galacturonic acid, etc. Historically, the name hexuronic acid was originally given to ascorbic acid.Coagulants: Agents that cause clotting.Polyanhydrides: Anhydride polymers with a repeating structure of RC(=O)OC(=O)R. They readily hydrolyze in water making them useful for DELAYED-ACTION PREPARATIONS.Glucuronic Acid: A sugar acid formed by the oxidation of the C-6 carbon of GLUCOSE. In addition to being a key intermediate metabolite of the uronic acid pathway, glucuronic acid also plays a role in the detoxification of certain drugs and toxins by conjugating with them to form GLUCURONIDES.Surface Properties: Characteristics or attributes of the outer boundaries of objects, including molecules.Dosage Forms: Completed forms of the pharmaceutical preparation in which prescribed doses of medication are included. They are designed to resist action by gastric fluids, prevent vomiting and nausea, reduce or alleviate the undesirable taste and smells associated with oral administration, achieve a high concentration of drug at target site, or produce a delayed or long-acting drug effect.Solubility: The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Polyesters: Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours.Polymethacrylic Acids: Poly-2-methylpropenoic acids. Used in the manufacture of methacrylate resins and plastics in the form of pellets and granules, as absorbent for biological materials and as filters; also as biological membranes and as hydrogens. Synonyms: methylacrylate polymer; poly(methylacrylate); acrylic acid methyl ester polymer.Excipients: Usually inert substances added to a prescription in order to provide suitable consistency to the dosage form. These include binders, matrix, base or diluent in pills, tablets, creams, salves, etc.AcrylatesEndothelium, Vascular: Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.Powder Diffraction: Method of using a polycrystalline powder and Rietveld refinement (LEAST SQUARES ANALYSIS) of X-RAY DIFFRACTION or NEUTRON DIFFRACTION. It circumvents the difficulties of producing single large crystals.Porosity: Condition of having pores or open spaces. This often refers to bones, bone implants, or bone cements, but can refer to the porous state of any solid substance.Calorimetry, Differential Scanning: Differential thermal analysis in which the sample compartment of the apparatus is a differential calorimeter, allowing an exact measure of the heat of transition independent of the specific heat, thermal conductivity, and other variables of the sample.Alginates: Salts of alginic acid that are extracted from marine kelp and used to make dental impressions and as absorbent material for surgical dressings.Platelet Glycoprotein GPIb-IX Complex: Platelet membrane glycoprotein complex essential for normal platelet adhesion and clot formation at sites of vascular injury. It is composed of three polypeptides, GPIb alpha, GPIb beta, and GPIX. Glycoprotein Ib functions as a receptor for von Willebrand factor and for thrombin. Congenital deficiency of the GPIb-IX complex results in Bernard-Soulier syndrome. The platelet glycoprotein GPV associates with GPIb-IX and is also absent in Bernard-Soulier syndrome.Hydrogels: Water swollen, rigid, 3-dimensional network of cross-linked, hydrophilic macromolecules, 20-95% water. They are used in paints, printing inks, foodstuffs, pharmaceuticals, and cosmetics. (Grant & Hackh's Chemical Dictionary, 5th ed)Thrombosis: Formation and development of a thrombus or blood clot in the blood vessel.Lecithins: A complex mixture of PHOSPHOLIPIDS; GLYCOLIPIDS; and TRIGLYCERIDES; with substantial amounts of PHOSPHATIDYLCHOLINES; PHOSPHATIDYLETHANOLAMINES; and PHOSPHATIDYLINOSITOLS, which are sometimes loosely termed as 1,2-diacyl-3-phosphocholines. Lecithin is a component of the CELL MEMBRANE and commercially extracted from SOYBEANS and EGG YOLK. The emulsifying and surfactant properties are useful in FOOD ADDITIVES and for forming organogels (GELS).Magnetics: The study of MAGNETIC PHENOMENA.Biocompatible Materials: Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.Emulsions: Colloids formed by the combination of two immiscible liquids such as oil and water. Lipid-in-water emulsions are usually liquid, like milk or lotion. Water-in-lipid emulsions tend to be creams. The formation of emulsions may be aided by amphiphatic molecules that surround one component of the system to form MICELLES.Aerosols: Colloids with a gaseous dispersing phase and either liquid (fog) or solid (smoke) dispersed phase; used in fumigation or in inhalation therapy; may contain propellant agents.Antibodies, Immobilized: Antibodies that are chemically bound to a substrate material which renders their location fixed.Hydrogel: A network of cross-linked hydrophilic macromolecules used in biomedical applications.Desiccation: Removal of moisture from a substance (chemical, food, tissue, etc.).Administration, Inhalation: The administration of drugs by the respiratory route. It includes insufflation into the respiratory tract.Thrombin: An enzyme formed from PROTHROMBIN that converts FIBRINOGEN to FIBRIN.Swiss 3T3 Cells: A cell line established in 1962 from disaggregated Swiss albino mouse embryos. This fibroblast cell line is extremely popular in research.Phosphatidylserines: Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.Thermogravimetry: Technique whereby the weight of a sample can be followed over a period of time while its temperature is being changed (usually increased at a constant rate).P-Selectin: Cell adhesion molecule and CD antigen that mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells.Polyethylene Glycols: Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.Microfluidic Analytical Techniques: Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.Spectroscopy, Fourier Transform Infrared: A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.Electroplating: Coating with a metal or alloy by electrolysis.Miniaturization: The design or construction of objects greatly reduced in scale.Materials Testing: The testing of materials and devices, especially those used for PROSTHESES AND IMPLANTS; SUTURES; TISSUE ADHESIVES; etc., for hardness, strength, durability, safety, efficacy, and biocompatibility.Antigens, CD31: Cell adhesion molecules present on virtually all monocytes, platelets, and granulocytes. CD31 is highly expressed on endothelial cells and concentrated at the junctions between them.Nanostructures: Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.Carboxymethylcellulose Sodium: A cellulose derivative which is a beta-(1,4)-D-glucopyranose polymer. It is used as a bulk laxative and as an emulsifier and thickener in cosmetics and pharmaceuticals and as a stabilizer for reagents.Vaccines: Suspensions of killed or attenuated microorganisms (bacteria, viruses, fungi, protozoa), antigenic proteins, synthetic constructs, or other bio-molecular derivatives, administered for the prevention, amelioration, or treatment of infectious and other diseases.Methylene Chloride: A chlorinated hydrocarbon that has been used as an inhalation anesthetic and acts as a narcotic in high concentrations. Its primary use is as a solvent in manufacturing and food technology.Microfluidics: The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.Gelatin: A product formed from skin, white connective tissue, or bone COLLAGEN. It is used as a protein food adjuvant, plasma substitute, hemostatic, suspending agent in pharmaceutical preparations, and in the manufacturing of capsules and suppositories.Dermatitis, Phototoxic: A nonimmunologic, chemically induced type of photosensitivity producing a sometimes vesiculating dermatitis. It results in hyperpigmentation and desquamation of the light-exposed areas of the skin.Nucleic Acids: High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.Blood Preservation: The process by which blood or its components are kept viable outside of the organism from which they are derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).Caproates: Derivatives of caproic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a carboxy terminated six carbon aliphatic structure.Methylcellulose: Methylester of cellulose. Methylcellulose is used as an emulsifying and suspending agent in cosmetics, pharmaceutics and the chemical industry. It is used therapeutically as a bulk laxative.Colloids: Two-phase systems in which one is uniformly dispersed in another as particles small enough so they cannot be filtered or will not settle out. The dispersing or continuous phase or medium envelops the particles of the discontinuous phase. All three states of matter can form colloids among each other.Exosomes: A type of extracellular vesicle, containing RNA and proteins, that is secreted into the extracellular space by EXOCYTOSIS when MULTIVESICULAR BODIES fuse with the PLASMA MEMBRANE.Pharmaceutical Solutions: Homogeneous liquid preparations that contain one or more chemical substances dissolved, i.e., molecularly dispersed, in a suitable solvent or mixture of mutually miscible solvents. For reasons of their ingredients, method of preparation, or use, they do not fall into another group of products.Nanotechnology: The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Biological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.Adhesiveness: A property of the surface of an object that makes it stick to another surface.Blood Coagulation Factors: Endogenous substances, usually proteins, that are involved in the blood coagulation process.Chromatography, Supercritical Fluid: A CHROMATOGRAPHY method using supercritical fluid, usually carbon dioxide under very high pressure (around 73 atmospheres or 1070 psi at room temperature) as the mobile phase. Other solvents are sometimes added as modifiers. This is used both for analytical (SFC) and extraction (SFE) purposes.Biolistics: Techniques where DNA is delivered directly into organelles at high speed using projectiles coated with nucleic acid, shot from a helium-powered gun (gene gun). One of these techniques involves immunization by DNA VACCINES, which delivers DNA-coated gold beads to the epidermis.Leukocytes: White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).Administration, Oral: The giving of drugs, chemicals, or other substances by mouth.Plasma: The residual portion of BLOOD that is left after removal of BLOOD CELLS by CENTRIFUGATION without prior BLOOD COAGULATION.Transport Vesicles: Vesicles that are involved in shuttling cargo from the interior of the cell to the cell surface, from the cell surface to the interior, across the cell or around the cell to various locations.Silicon Dioxide: Transparent, tasteless crystals found in nature as agate, amethyst, chalcedony, cristobalite, flint, sand, QUARTZ, and tridymite. The compound is insoluble in water or acids except hydrofluoric acid.Thrombophilia: A disorder of HEMOSTASIS in which there is a tendency for the occurrence of THROMBOSIS.Microtechnology: Manufacturing technology for making microscopic devices in the micrometer range (typically 1-100 micrometers), such as integrated circuits or MEMS. The process usually involves replication and parallel fabrication of hundreds or millions of identical structures using various thin film deposition techniques and carried out in environmentally-controlled clean rooms.Miotics: Agents causing contraction of the pupil of the eye. Some sources use the term miotics only for the parasympathomimetics but any drug used to induce miosis is included here.Ferric Compounds: Inorganic or organic compounds containing trivalent iron.Wettability: The quality or state of being wettable or the degree to which something can be wet. This is also the ability of any solid surface to be wetted when in contact with a liquid whose surface tension is reduced so that the liquid spreads over the surface of the solid.Fluorescein-5-isothiocyanate: Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.Biological Availability: The extent to which the active ingredient of a drug dosage form becomes available at the site of drug action or in a biological medium believed to reflect accessibility to a site of action.Serum Albumin, Bovine: Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)Sodium Tetradecyl Sulfate: An anionic surface-active agent used for its wetting properties in industry and used in medicine as an irritant and sclerosing agent for hemorrhoids and varicose veins.Monocytes: Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.Disposable Equipment: Apparatus, devices, or supplies intended for one-time or temporary use.Dendrimers: Tree-like, highly branched, polymeric compounds. They grow three-dimensionally by the addition of shells of branched molecules to a central core. The overall globular shape and presence of cavities gives potential as drug carriers and CONTRAST AGENTS.Lab-On-A-Chip Devices: Microdevices that combine microfluidics technology with electrical and/or mechanical functions for analyzing very small fluid volumes. They consist of microchannels etched into substrates made of silicon, glass, or polymer using processes similar to photolithography. The test fluids in the channels can then interact with different elements such as electrodes, photodetectors, chemical sensors, pumps, and valves.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Fullerenes: A polyhedral CARBON structure composed of around 60-80 carbon atoms in pentagon and hexagon configuration. They are named after Buckminster Fuller because of structural resemblance to geodesic domes. Fullerenes can be made in high temperature such as arc discharge in an inert atmosphere.Mannitol: A diuretic and renal diagnostic aid related to sorbitol. It has little significant energy value as it is largely eliminated from the body before any metabolism can take place. It can be used to treat oliguria associated with kidney failure or other manifestations of inadequate renal function and has been used for determination of glomerular filtration rate. Mannitol is also commonly used as a research tool in cell biological studies, usually to control osmolarity.X-Ray Diffraction: The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Inflammation: A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.Flow Injection Analysis: The analysis of a chemical substance by inserting a sample into a carrier stream of reagent using a sample injection valve that propels the sample downstream where mixing occurs in a coiled tube, then passes into a flow-through detector and a recorder or other data handling device.Case-Control Studies: Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Human Umbilical Vein Endothelial Cells: Endothelial cells that line venous vessels of the UMBILICAL CORD.Fluorescein: A phthalic indicator dye that appears yellow-green in normal tear film and bright green in a more alkaline medium such as the aqueous humor.Cytophagocytosis: The engulfment and degradation of cells by other cells.Drug Stability: The chemical and physical integrity of a pharmaceutical product.Solvents: Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)Mice, Inbred BALB CSuspensions: Colloids with liquid continuous phase and solid dispersed phase; the term is used loosely also for solid-in-gas (AEROSOLS) and other colloidal systems; water-insoluble drugs may be given as suspensions.Equipment Design: Methods of creating machines and devices.Glycolates: Derivatives of ACETIC ACID which contain an hydroxy group attached to the methyl carbon.Spasm: An involuntary contraction of a muscle or group of muscles. Spasms may involve SKELETAL MUSCLE or SMOOTH MUSCLE.Nanowires: Nanometer-scale wires made of materials that conduct electricity. They can be coated with molecules such as antibodies that will bind to proteins and other substances.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Tablets, Enteric-Coated: Tablets coated with material that delays release of the medication until after they leave the stomach. (Dorland, 28th ed)Mefenamic Acid: A non-steroidal anti-inflammatory agent with analgesic, anti-inflammatory, and antipyretic properties. It is an inhibitor of cyclooxygenase.Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.Erythrocytes: Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.Synovial Fluid: The clear, viscous fluid secreted by the SYNOVIAL MEMBRANE. It contains mucin, albumin, fat, and mineral salts and serves to lubricate joints.Light Coagulation: The coagulation of tissue by an intense beam of light, including laser (LASER COAGULATION). In the eye it is used in the treatment of retinal detachments, retinal holes, aneurysms, hemorrhages, and malignant and benign neoplasms. (Dictionary of Visual Science, 3d ed)

Tissue factor activity is increased in a combined platelet and microparticle sample from cancer patients. (1/383)

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Microparticles are vectors of paradoxical information in vascular cells including the endothelium: role in health and diseases. (2/383)

Both inflammation and thrombosis can be orchestrated by the interactions between circulating cells, such as leukocytes and platelets, with vascular, endothelial and smooth muscle cells, which, during activation or apoptosis, can release circulating microparticles (MPs). Indeed, MPs are membrane vesicles with procoagulant and proinflammatory properties. MPs are present in blood from healthy individuals and in patients under several pathological states, for instance sepsis, preeclampsia, Crohn's disease and diabetes, strengthening the notion that MPs may play a role in these diseases. Circulating MPs or those generated in vitro from apoptotic T cells display deleterious effects on endothelial and/or vasomotor function. In contrast, MPs might be protective to endothelial cells. We have shown that MPs harboring the morphogen sonic hedgehog may represent a new therapeutic approach against endothelial dysfunction during acute severe endothelial injury. Indeed, these types of MPs induce NO release, decrease production of reactive oxygen species and induce angiogenesis from endothelial cells. This protective role for the endothelium was confirmed also by their in vivo injection in mice in which they were also able to reverse endothelial dysfunction in a model of heart ischemia/reperfusion. On the contrary, MPs from preeclamptic women compared to those from normal pregnant women showed pro-inflammatory properties in the vascular wall inducing vascular hyporeactivity in vessels from humans and mice. These effects were associated with complex interactions between NO and cyclooxygenase systems via endothelial cell activation. Altogether, these findings suggest that MPs can be considered as vectors of biological messages for vascular homeostasis, during immunity and inflammation.  (+info)

The role of cell death in the pathogenesis of autoimmune disease: HMGB1 and microparticles as intercellular mediators of inflammation. (3/383)

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The relationship between plasma microparticles and disease manifestations in patients with systemic sclerosis. (4/383)

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Measurement of platelet-derived microparticle levels in the chronic phase of cerebral infarction using an enzyme-linked immunosorbent assay. (5/383)

Assessment of platelet function is a critical component of the treatment and secondary prevention of cerebral infarction, and measurement of platelet-derived microparticle (PDMP) levels using flow cytometry may be a good indicator of platelet function. However, the flow cytometric analysis is not feasible in a variety of clinical situations. The goal of the present study was to measure PDMP levels using an enzyme-linked immunosorbent assay (ELISA) in chronic cerebral infarction patients and to determine the utility of PDMP level measurement for the monitoring of the effect of cilostazol and aspirin. A crossover study was performed using 4-weeks of aspirin (100 mg/day) and 4-weeks of cilostazol (200 mg/day) in 18 patients. PDMP levels were also measured in 20 volunteers as controls. Experiments demonstrated that PDMP levels were significantly higher in chronic cerebral infarction patients (median 8.8 U/ml, interquartile range 5.1-14.9 U/ml, n=18) than in controls (median 5.5 U/ml, interquartile range 5.0-8.2 U/ml, n=20) (P=0.047). PDMP levels did not decrease after therapy with either aspirin (median 10.9 U/ml, interquartile range 6.2-17.9 U/ml, n=12) or cilostazol (median 9.2 U/ml, interquartile range 6.1-14.3 U/ml, n=12) compared with baseline PDMP levels in the 12 patients who completed this trial (median 11.4 U/ml, interquartile range 5.2-23.7 U/ml, n=12). There were no significant differences in PDMP levels between aspirin and cilostazol (P=0.61). In conclusion, PDMP levels as measured by ELISA were increased in patients with chronic cerebral infarction regardless of the anti-platelet therapy. This methodology may be a useful strategy of assessing platelet function in chronic cerebral infarction patients.  (+info)

Megakaryocyte-derived microparticles: direct visualization and distinction from platelet-derived microparticles. (6/383)

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Dynamics of circulating microparticles in liver transplant patients. (7/383)

BACKGROUND & AIMS: Microparticles are small membrane vesicles released from the cell plasma membrane, particularly in cell stress, apoptosis and altered cellular viability. Hepatocellular carcinoma (HCC) is a hypervascular neoplasm with high levels of apoptosis and necrosis. We investigated the levels of circulating microparticles of both tumor and endothelial origins in liver transplant patients with hepatitis C (HepC) cirrhosis with and without HCC and compared them with healthy people and patients with partial hepatectomy. METHODS: Using immunolabeling of microparticles of different origin and flow cytometry-based enumeration of microparticles, the levels of circulating microparticles were studied in 8 patients with HepC and 8 patients with both HepC and HCC before and within two weeks after the transplant. RESULTS: The initial levels of circulating microparticles were increased in patients with HepC and HCC as compared to patients with HepC alone. They were also increased in liver transplant patients as compared to patients with partial hepatectomy or healthy people. Levels of circulating microparticles were dynamically changing after the transplant, showing an initial increase with a subsequent decrease by the end of the second week after surgery. In some patients with a complicated clinical outcome, the levels of microparticles were continuously increasing after the surgery. CONCLUSION: The levels of circulating microparticles of endothelial and hepatic origin in liver transplant patients dynamically change after surgery and correlate with the clinical outcome. Perspectively, the levels of circulating microparticles may be used in clinical practice as a marker of the functional status of the transplanted liver.  (+info)

Diagnostic role of endothelial microparticles in vasculitis. (8/383)

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After long controversy, it is now recognized that essentially all neurodegenerative diseases have inflammatory components [1]. Many other neurological disorders are exacerbated by inflammation, including the progressive impairment following stroke or traumatic brain injury (TBI). It has been known that levels of circulating cell-derived microparticles (MP) are generally increased in inflammatory states, making them useful as biomarkers. More recently, certain species of MP or exo- somes have been implicated as causative agents in neuroinflammation, to be reviewed.. L.L. Horstman, BS • W. Jy, PhD (*) • Y.S. Ahn, MD. University of Miami, Department of Medicine (Wallace H. Coulter Platelet Laboratory), 1600 NW 10TH Ave., Mail Code R36A, Miami, FL 33136, USA e-mail: This email address is being protected from spam bots, you need Javascript enabled to view it © Springer International Publishing AG 2017. A. Minagar, J.S. Alexander (eds.), Inflammatory Disorders of the Nervous System, Current ...
0175]1. Hugel B, Martinez M C, Kunzelmann C, Freyssinet J M. Membrane microparticles: two sides of the coin. Physiology (Bethesda, Md. February 2005; 20:22-27. [0176]2. Mallat Z, Benamer H, Hugel B, Benessiano J, Steg P G, Freyssinet J M, Tedgui A. Elevated levels of shed membrane microparticles with procoagulant potential in the peripheral circulating blood of patients with acute coronary syndromes. Circulation. Feb. 29 2000; 101(8):841-843. [0177]3. Nieuwland R, Berckmans R J, Rotteveel-Eijkman R C, Maquelin K N, Roozendaal K J, Jansen P G, ten Have K, Eijsman L, Hack C E, Sturk A. Cell-derived microparticles generated in patients during cardiopulmonary bypass are highly procoagulant. Circulation. Nov. 18 1997; 96(10):3534-3541. [0178]4. Tesse A, Martinez M C, Hugel B, Chalupsky K, Muller C D, Meziani F, Mitolo-Chieppa D, Freyssinet J M, Andriantsitohaina R. Upregulation of proinflammatory proteins through NF-kappaB pathway by shed membrane microparticles results in vascular hyporeactivity. ...
TY - JOUR. T1 - Phenotypic assessment of endothelial microparticles in patients with heart failure and after heart transplantation. T2 - Switch from cell activation to apoptosis. AU - Garcia, Santiago. AU - Chirinos, Julio. AU - Jimenez, Javier. AU - Del Carpio Muñoz, Freddy. AU - Canoniero, Mariana. AU - Jy, Wenche. AU - Jimenez, Joaquin J. AU - Horstman, Lawrence. AU - Ahn, Yeon. PY - 2005/12/1. Y1 - 2005/12/1. N2 - Background: Endothelial microparticles (EMPs) are sub-microscopic membrane vesicles that are shed from the surface of endothelial cells during activation, injury and/or apoptosis. Endothelial cells release phenotypically and quantitatively distinct endothelial microparticles (EMPs) in activation and apoptosis. Therefore, the phenotypic assessment of EMPs can provide useful information reflecting the nature of endothelial injury. We tested the hypothesis that heart transplantation (HT) modifies the pattern of endothelial injury seen in patients with congestive heart failure (CHF). ...
Acute HIV-1 infection results in dysregulated immunity, which contributes to poor control of viral infection. DCs are key regulators of both adaptive and innate immune responses needed for controlling HIV-1, and we surmised that factors elicited during acute HIV-1 infection might impede DC function. We derived immature DCs from healthy donor peripheral blood monocytes and treated them with plasma from uninfected control donors and donors with acute HIV-1 infections. We found that the plasma from patients with HIV specifically inhibited DC function. This suppression was mediated by elevated apoptotic microparticles derived from dying cells during acute HIV-1 infection. Apoptotic microparticles bound to and inhibited DCs through the hyaluronate receptor CD44. These data suggest that targeting this CD44-mediated inhibition by apoptotic microparticles could be a novel strategy to potentiate DC activation of HIV-specific immunity.
TY - JOUR. T1 - Circulating endothelial microparticles as a measure of early lung destruction in cigarette smokers. AU - Gordon, Cynthia. AU - Gudi, Kirana. AU - Krause, Anja. AU - Sackrowitz, Rachel. AU - Harvey, Ben Gary. AU - Strulovici-Barel, Yael. AU - Mezey, Jason G.. AU - Crystal, Ronald. PY - 2011/7/15. Y1 - 2011/7/15. N2 - Rationale: There is increasing evidence thatemphysemais associated with primary loss of pulmonary capillary endothelium. Plasma levels of endothelial microparticles (EMPs), small vesicles released from activated or apoptotic endothelial cells, are elevated in vascularrelated disorders. Objectives: To evaluate whether plasma EMP levels are elevated in smokers with early lung destruction as assessed by normal spirometry but reduced diffusing capacity of the lung for carbon monoxide (DLCO). Methods: Lung health was assessed by pulmonary function tests (PFTs: spirometry, total lung capacity, DLCO) and chest X-ray; smoking status was assessed by urine nicotine and ...
The results of these studies demonstrate that calcineurin inhibitors cause endothelial cells to increase the number of microparticles released into the circulation from the cell surface. Calcineurin inhibitors also alter the composition of the microparticles such that they become complement activating. We found that alternative pathway-deficient mice are protected from CsA-induced renal and vascular injury. We also found that endothelial microparticles cause injury of unmanipulated endothelial cells in vitro, and they cause mesangial proliferation and complement activation when passively transferred into wild-type mice in vivo. These results demonstrate that CsA-induced endothelial microparticles can cause bystander injury of endothelial cells, and they promote glomerular complement activation and mesangial expansion. Furthermore, preliminary data from human transplant patients indicate that treatment of these patients with tacrolimus is also associated with generation of endothelial ...
Maturation (MP) or polishing ponds are widely used throughout the world as a final or tertiary wastewater treatment process for effluent quality improvement from secondary biological treatment systems, including FPs. MPs can be expected to reduce total suspended solids (TSS), ammonia, nitrate and phosphorus levels in effluent, but the primary function of MPs in most parts of the world is the removal of pathogenic organisms. A number of mechanisms in MPs are responsible for pathogen reduction, including settlement, natural die off (a function of time and temperature), elevated pH, UV radiation and predation. The design, size and number (usually in series) of MPs are typically based on the desired bacteriological quality of the final effluent. MPs are typically designed at the same depth as facultative ponds (2 to 5 feet (0.7 to 1.5 m)). Retention times in MPs are typically designed at 10 to 14 days for pathogen reduction; however, shorter retention times are required for TSS removal (four to five ...
Microparticles are membrane vesicles with procoagulant and proinflammatory properties released during cell activation or apoptosis. Microparticles from monocytes have been implicated in atherosclerosis and vascular inflammation, but their direct effects on endothelial cells are not completely elucidated. The present study was designed to dissect the signaling pathways of monocytic microparticles in endothelial cells with respect to both NO pathway and reactive oxygen species. Microparticles were produced by treatment of human monocytic cell line THP-1 with the apoptotic agent VP-16. Human endothelial cells were treated with monocytic microparticles and then, we studied their effects on nitrosative and oxidative stresses. Incubation of human endothelial cells with microparticles enhanced the production of NO without affecting superoxide anions generation. Microparticles did not affect endothelial NO synthase expression and its phosphorylation. Interestingly, microparticles decreased caveolin-1 ...
Microparticles are a heterogeneous population of membrane-coated vesicles which can be released from virtually all cell types during activation or apoptosis. Release occurs from the cell surface in an exogenous budding process involving local rearrangement of the cytoskeleton. Given their origin, these particles can be identified by staining for cell surface markers and annexin V. As shown in in vitro studies, microparticles may represent a novel subcellular element for intercellular communication in inflammation. Thus, microparticles can transfer chemokine receptors and arachidonic acid between cells, activate complement, promote leukocyte rolling and stimulate the release of pro-inflammatory mediators. Under certain conditions, however, microparticles may also exert anti-inflammatory properties by inducing immune cell apoptosis and the production of anti-inflammatory mediators. Microparticles may play an important role in the pathogenesis of rheumatologic diseases as evidenced by their elevation in
Simoncini S, Njock MS, Robert S, Camoin-Jau L, Sampol J, Harlé JR, Nguyen C, Dignat-George F, Anfosso F. Circ Res. 2009 Apr 24 ;104 (8):943-51 (...)
Oxidative Medicine and Cellular Longevity is a unique peer-reviewed, Open Access journal that publishes original research and review articles dealing with the cellular and molecular mechanisms of oxidative stress in the nervous system and related organ systems in relation to aging, immune function, vascular biology, metabolism, cellular survival and cellular longevity. Oxidative stress impacts almost all acute and chronic progressive disorders and on a cellular basis is intimately linked to aging, cardiovascular disease, cancer, immune function, metabolism and neurodegeneration. The journal fills a significant void in todays scientific literature and serves as an international forum for the scientific community worldwide to translate pioneering
Microparticles (MPs) are fragments of cell membranes released from stimulated or apoptotic cells that have long been considered innocent remnants of cell destruction. Evidence is accumulating, however, that the circulating and tissue-derived MPs may elicit a strong thrombogenic and inflammatory response (1). In addition to MPs, activated cells can also release smaller vesicle-denominated exosomes that are relatively unexplored in the research area of vascular biology (2).. Circulating MPs are being explored as a potential source for biomarker discovery. Platelet MPs have been associated with clinically evident atherosclerotic disease in diabetes type II patients (3), and leukocyte-derived MPs are predictive of subclinical atherosclerosis in asymptomatic patients (4).. Experimental and clinical data consistently point to a causal role for MPs in atherosclerosis development and progression. Recent reports revealed their contribution to hemostatic and inflammatory responses, vascular remodeling and ...
Background: Early and non-invasive biomarkers of kidney damage are needed to identify hypertensive patients at risk for kidney damage. Urinary micropaticles (UMPs) have gained significant attention as potential novel biomarkers for kidney damage, and have already been identified in pre-albuminuric diabetic glomerular injury. These vesicles are less than 1 micron in size and carry markers of the parent cell. We hypothesized that podocyte derived UMPs are elevated in angiotensin II-induced hypertension (HTN). Methods: Primary podocytes were isolated and grown in culture. Wild-type mice were treated with AII (400ng/kg/min) via mini-osmotic pumps. Untreated WT mice served as controls. 24 hour urines were collected after 5 days of AII treatment. Enumeration and phenotyping of MPs was done of podocyte culture supernatant and urine. Podocalyxin (Pcal), podoplanin (Ppla) and annexin 5 (AV) were used as surface markers.. Result: Pcal and Ppla positive MPs as well as AV positive and negative MPs were ...
CA153 : The instrument used is the Roche Cobas. The Roche cancer antigen 15-3 (CA 15-3) method is a sandwich electrochemiluminescence immunoassay that employs a biotinylated monoclonal CA 15-3-specific antibody and a monoclonal CA 15-3-specific antibody. CA 15-3 in the automatically prediluted specimen reacts with both the biotinylated monoclonal CA 15-3-specific antibody (mouse) and the monoclonal CA 15-3-specific antibody (mouse) labeled with a ruthenium complex, forming a sandwich complex. Streptavidin-coated microparticles are added and the mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell. Application of voltage to the electrode induces the chemiluminescent emission, which is then measured.(Package insert: Roche CA 15-3 reagent, Roche Diagnostic Corp., Indianapolis, IN 2010-09, V14)
BOSTON, Massachusetts (PNN) - March 28, 2017 - A team of scientists at the Boston Childrens Hospital have invented what is being considered one the greatest medical breakthroughs in recent years. They have designed a microparticle that can be injected into a persons bloodstream that can quickly oxygenate his or her blood. This will even work if the ability to breathe has been restricted, or even cut off entirely.. This finding has the potential to save millions of lives every year. The microparticles can keep an object alive for up to 30 minutes after respiratory failure. This is accomplished through an injection into the patients veins. Once injected, the microparticles can oxygenate the blood to near normal levels. This has countless potential uses as it allows life to continue when oxygen is needed but unavailable. For medical personnel, this is just enough time to avoid risking a heart attack or permanent brain injury when oxygen is restricted or cut off to patient.. Dr. John Kheir, who ...
2015. Ghelani H, Razmovski-Naumovski V, Kyada AV, Parekh HS, Li GQ, Nammi S. Myoglobin and low density lipoprotein glycation in cardiac complications of diabetes: Current perspectives and therapeutic options. Indian J Med Res. 2015. In Press. Jadeja R, Devkar RV, Nammi S. Hepatoprotective potential of herbal medicine. Evid-Based Compl Alt. [Editorial]. 2015;2015.. Kam A, Li KM, Razmovski-Naumovski V, Nammi S, Chan K, Grau GE, et al. Curcumin reduces tumour necrosis factor-enhanced annexin V-positive microparticle release in human vascular endothelial cells. J Pharm Pharm Sci. 2015;18(4):424-33.. Thounaojam MC, Nammi S, Jadeja R. Natural products for the treatment of obesity, metabolic syndrome, and type 2 diabetes 2014. Evid-Based Compl Alt. [Editorial]. 2015;2015. DOI: 10.1155/2015/392681.. Yang WC, Chang CLT, Li CR, Nammi S, Cho WCS. Complementary and alternative medicine for diabetes 2014. Evid-Based Compl Alt. [Editorial]. 2015;2015. DOI: 10.1155/2015/685248.. 2014. Li Y, Tran VH, Kota BP, ...
TY - JOUR. T1 - Modification of molecular conformation of spray-dried whey protein microparticles improving digestibility and release characteristics. AU - Ye, Qianyu. AU - Woo, Meng Wai. AU - Selomulya, Cordelia. PY - 2019/5/15. Y1 - 2019/5/15. N2 - This study reports on the preparation of riboflavin-loaded whey protein isolate (WPI) microparticles, using desolvation and then spray drying. Ethanol desolvation led to the exposure of embedded hydrophobic amino acids of WPI to riboflavin, facilitating the formation of riboflavin-WPI complexes. The extent of desolvation and cross-linking influenced the morphology of the spray-dried microparticles, while the moisture content of microparticles decreased with desolvation and increased with crosslinking. The modification of WPI conformation upon desolvation could be retained in the dry state via spray drying. The gastric resistance, release site and release characteristics of microparticles were readily adjusted by varying the ethanol and calcium ion ...
Overall research in my lab explores the molecular mechanisms of cellular signal transduction, with several related foci: 1) intercellular signaling in platelet-tumor interactions, including the contributions of Ras family small G proteins; 2) the contributions of protein trafficking to the signaling and oncogenic functions of Ras proteins; 3) Ras signaling in platelets, regulating thrombosis and hemostasis. My long term goal is to reach a new understanding of cellular information processing and its contributions to signal responses and to translate these findings into pre-clinical approaches.. 1. Intercellular signaling in platelet-tumor interactions.. We recently discovered that platelet microparticles - small extracellular vesicles released by blood platelets into plasma - infiltrate solid tumors by means of the unusual permeability of tumor vasculature, whereupon the platelet microparticles anchor to tumor cells and transfer genetic information in the form of platelet-derived microRNAs ...
VEGF/VEGFR inhibitors, used as anti-angiogenic drugs to treat cancer, induce severe hypertension. Molecular mechanisms are unclear, but nitric oxide (NO) and oxidative stress may be involved. We questioned whether reactive oxygen species (ROS) and Ang II also play a role in VEGF inhibitor-induced vascular dysfunction. Human microvascular endothelial cells (HMECs) were stimulated with vatalanib (VAT-VEGFR inhibitor) and gefitinib (GEF-EGFR inhibitor) in the absence/presence of Ang II. Activation of eNOS and MAPKs were assessed by immunoblotting. Antioxidant enzyme mRNA was analysed by qPCR. Endothelial microparticles, biomarkers of endothelial damage, tend to increase in subjects treated with VEGFR inhibitors. Phosphorylation of eNOS (28.3% ± 7.1) was decreased by VAT (p , 0.05). VAT decreased mRNA expression of Nox4 (0.5 ± 0.2) and H2O2-regulating antioxidants enzymes such as catalase (0.4 ± 0.1) and glutathione peroxidase (0.4 ± 0.1), while increased mRNA levels of Nox5 (3.35 ± 1.1) (p , ...
Background: Neopetrosiamide A (NeoA) is a 28-amino acid tricyclic peptide originally isolated from a marine sponge as a tumor cell invasion inhibitor whose mechanism of action is unknown. Methodology/Principal Findings: We show that NeoA reversibly inhibits tumor cell adhesion, disassembles focal adhesions in pre-attached cells, and decreases the level of beta 1 integrin subunits on the cell surface. NeoA also induces the formation of dynamic, membrane-bound protrusions on the surface of treated cells and the release of membrane-bound vesicles into the culture medium. Proteomic analysis indicates that the vesicles contain EGF and transferrin receptors as well as a number of proteins involved in adhesion and migration including: beta 1 integrin and numerous alpha integrin subunits; actin and actin-binding proteins such as cofilin, moesin and myosin 1C; and membrane modulating eps15 homology domain (EHD) proteins. Surface labeling, trafficking inhibition, and real-time imaging experiments all ...
M. ABBAS, L. JESEL, C. AUGER, L. AMOURA, N. MESSAS, G. MANIN, C. RUMIG, A.J. LEON-GONZALEZ, T.P. RIBEIRO, G.C. SILVA, R. ABOU-MERHI, E. HAMADE, M. HECKER, Y. GEORG, N. CHAKFE, P. OHLMANN, V.B. SCHINI-KERTH, F. TOTI & O. MOREL. Endothelial microparticles from acute coronary syndrome patients induce premature coronary artery endothelial cells ageing and thrombogenicity: role of the Ang II/AT1 receptor/NADPH oxidase-mediated activation of MAPKs and PI3-kinase pathways. Circulation 135, 280-296, 2017 - IF 17.20 - ...
Pitha J, Dorazilova Z, Melenovsky V, Kralova Lesna I, Stavek P, Stepankova J, Urban M, Maly J, Netuka I. The impact of left ventricle assist device on circulating endothelial microparticles - pilot study. Neuro Endocrinol Lett. 2012 Jan; 33(Suppl 2): 68-72 ...
A beam of accelerated microparticles of substantially uniform velocity is passed transversely through an intense pulsed laser beam. In traversing the laser beam, the microparticles are thermally evapo
TGF-β3 is enzymatically immobilized by transglutaminase-2 action to poly(L-lactic acid) microparticles coated with collagen II. Microparticles are then encapsulated with stem cells inside liquified spherical compartments enfolded with a permselective shell through layer-by-layer adsorption. Magnetic nanoparticles are electrostatically bound to the multilayered shell, conferring magnetic-response ability. The goal of this study is to engineer a closed environment inside which encapsulated stem cells would undergo a self-regulated chondrogenesis. To test this hypothesis, capsules are cultured in chondrogenic differentiation medium without TGF-β3. Their biological outcome is compared with capsules encapsulating microparticles without TGF-β3 immobilization and cultured in normal chondrogenic differentiation medium containing soluble TGF-β3. Glycosaminoglycans quantification demosntrates that similar chondrogenesis levels are achieved. Moreover, collagen fibrils resembling the native ...
Summary of Facts and Submissions. I. This appeal is from the interlocutory decision of the Opposition Division concerning maintenance of European patent No. 1 328 683 in amended form on the basis of the then pending main request. The independent Claim 1 reads:. 1. A process for making paper or paper board comprising forming a cellulosic suspension, flocculating the suspension, draining the suspension on a screen to form a sheet and then drying the sheet,. characterised in that. the suspension is flocculated using a flocculating system comprising a siliceous material and organic microparticles which have an unswollen particle diameter of less than 750 nanometers,. wherein a further flocculating material is included into the cellulosic suspension before adding the polymeric microparticles and siliceous material,. and in which the flocculating material is cationic and is a natural or synthetic polymer,. in which the microparticles are made from anionic copolymers comprising from 0 to 99 parts, by ...
A novel microparticle preparation comprising microparticles of a polymer which contain a drug and are coated with a film of an agent for preventing aggregation of the microparticles is disclosed. The preparation is produced by spraying a solution of a polymer containing a drug and an aqueous solution of an agent for preventing aggregation of the microparticles separately from different nozzles and contacting them with each other in a spray dryer.
The argument that we must do everything a Minister demands because he has been democratically chosen does not stand up to close inspection. MPs are not chosen by the people - they are chosen by their local constituency parties: thirty-five men in grubby raincoats or thirty-five women in silly hats. The further selection process is equally a nonsense: there are only 630 MPs and a party with just over 300 MPs forms a government and of these 300, 100 are too old and too silly to be ministers and 100 too young and too callow. Therefore there are about 100 MPs to fill 100 government posts. Effectively no choice at all ...
Macrophages (MPs) are heterogeneous, multifunctional, myeloid-derived leukocytes that are part of the innate immune system, playing wide-ranging critical roles in basic biological activities, including maintenance of tissue homeostasis involving clearance of microbial pathogens. Tumor-associated MPs …
Chepkwony said that they wanted the structure of the National Parliament replicated in county assemblies so that if MPs are appointed as Ministers then MCAs are also appointed as CECs.. "In the coming months we shall hold an AGM for all MCAs and we shall combine our recommendations and hand them to the BBI taskforce," he said.. On his part, the forum chairman Ndegwa Wahome said that they were against the proposal for regional governments noting that Kenyans were currently over-represented.. Wahome who is also the Nyandarua Assembly Speaker, said that the current system should be strengthened adding that wards should be funded as one way of addressing the challenges facing Wanjiku.. ...
Thirty years for The Three Profiteers and the Parasite Peer : John Wright a writer and specialist in crime is outraged along with millions of other people that only four MPs are to be charged out of the hundreds who fiddled their expenses and stole public money John reckons we should give them all thirty y
Hospital parking charges have been slammed for placing an unfair financial burden on carers. MPs are looking to change the law to force health bosses to ex...
This petition has received 10,759 signatures. our MPS are dragging there feet on this . i have a letter from boris johnston who wants to bring in new regulations o...
POSITEK 4G Microparticle Technology is the latest generation in Nalco microparticle innovation. This new programme is designed to promote drainage and retention without causing any negative effects caused by over-flocculation. | Ecolab
Vesicles are small membrane-bound structures containing materials for export. They combine with the outer cell membrane, releasing their contents. Epithelial cells form lining layers. ...
View Notes - fulltext37 from SPECTOGRAP 545 at AIB College of Business. Nano- and Microparticles of Organic Fluorescent Dyes Self-organization and Optical Properties SUZANNE FERY-FORGUES, MOUHAMMAD
ananyo writes Rabbits with blocked windpipes have been kept alive for up to 15 minutes without a single breath, after researchers injected oxygen-filled microparticles into the animals blood. Oxygenating the blood by bypassing the lungs in this way could save the lives of people with impaired brea...
phagosome: A membrane-bound vesicle found in a cell by an inward folding of the cell membrane to hold foreign matter taken into the cell by phagocytosis.
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
An Indian-origin researcher has created tiny polymer beads that can slowly release anti-inflammatory drugs, and break down into non-toxic components.
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies. ...
It has been thirty years since one of the authors (EJD) began a collaboration with Professor Milton Kerker at Clarkson University in Potsdam, New
University of Delaware, Doctoral Student, Chemical Engineering, 2013 - present. National Taiwan University, B.S., Chemical Engineering, 2008 - 2012. Research Project. Cell-derived microparticles (MPs) released from budding off the cell membrane of several type of mammalian cells. These MPs have been found playing an important role in cell-cell interaction by transffering RNA, protein and lipids. Based on the characteristics that these MP are target specific, biocompatible and immunologically inert, my project focus on engineering megakaryocyte-derived MPs as vesicles for exogenous-material-transfer, and investigating the key factors that manipulate the interactions between megakaryocyte-derived MPs with hematopoietic stem cell.. Technical Skills. ...
Extracellular vesicles are a heterogeneous group of membrane-enclosed vesicles, which play an important role in intercellular communication. Increasing number of studies have shown that tumor-derived extracellular vesicles might be involved in the transfer of oncogenic cargo (proteins, lipids, messenger RNA, microRNA, non-coding RNAs and DNA) through which cancer cells could shape the tumor microenvironment and influence tumor progression. Nasopharyngeal carcinoma-derived extracellular vesicles have also reported to facilitate tumor proliferation, metastasis and immune escape. Moreover, nasopharyngeal carcinoma-derived extracellular vesicles might serve as biomarkers for early diagnosis and therapeutic targets. The present review provides information on the biological and clinical significance of extracellular vesicles in tumors, especially in nasopharyngeal carcinoma ...
LRP-mediated agLDL uptake has proved to be one of the main mechanisms for intracellular lipid accumulation in human VSMCs,17-20 cells that have the capacity to secrete TF MP.22,27 In the present study, we hypothesized that agLDLs were able to induce cellular TF expression and activity. We found that both nLDL and agLDL induced TF mRNA after 2 hours of LDL exposure to the same level, in agreement with previous studies showing that nLDL and oxidized LDL induce TF mRNA in different cell types,12-14 which is likely unrelated to the receptor involved in LDL uptake. The increase in mRNA transcription levels induced by nLDL or agLDL did not require new protein synthesis but required a functional transcriptional mechanism. The increase in mRNA led to a strong increase in membrane-associated cellular TF protein expression. With regard to TF activity, the effect of agLDL on TF PCA, in contrast to a shorter time effect of nLDL, was maintained for 24 hours. The ability of nLDL to induce TF expression but ...
Background: Body fluids contain cell-derived vesicles ranging from 30 nm to 1 μm in diameter. The function, origin, and composition of these vesicles is disease dependent and therefore vesicles contain clinical information. The most common method to detect vesicles is flow cytometry, which guides vesicles through a laser beam in a hydrodynamically focused fluid stream. The unknown relationship between the measured light scattering intensity and the vesicle diameter resulted in unexplained contradictions between expected and observed results.. Methods: We combined light scattering measurements of polystyrene and silica beads with Mie calculations using an estimated refractive index of vesicles.. Results: We established the relationship between measured light scattering and the diameter of vesicles. We show that common gating strategies based on beads select vesicles (and cells) ranging from 800 to 2400 nm in diameter. For our flow cytometer, the smallest detectable silica beads were 204 nm, ...
Scientists report that adult cells reprogrammed to become primitive stem cells, called induced pluripotent stem cells (iPSCs), make more extracellular vesicles than other kinds of adult stem cells commonly used for this purpose in research. Extracellular vesicles are naturally abundant in many types of cells, which use the cargo-containing spheres to communicate with other cells. They are about one one-hundredth the diameter of a cell and can carry anything from fats and proteins to nucleic acids. When a cell releases an extracellular vesicle, other cells nearby slurp up the tiny packet and its contents, making it an attractive target for packaging treatments for diseased cells that are deteriorating or aging prematurely. To package a potential treatment in an extracellular vesicle, scientists typically use a cell called a mesenchymal stem cell, which is found among fat or bone marrow cells and gives rise to other fat and bone cells. Scientists genetically modify the stem cell to produce ...
This Research Topic addresses the emerging role of extracellular vesicles in cellular communications and immune responses. We will emphasize their use as biomarkers (research tool to better understand extracellular vesicle functions) and immune modulators (therapeutic tool to suppress or enhance immune responses). Extracellular Vesicles (EVs) are released in response to signals activating their parental cells. They are divided in two categories: small EVs (e.g. exosomes) and large EVs (e.g. microvesicles/ectosomes/microparticles). Small and large EVs differ in terms of size, release and budding mechanism, lipid and protein composition, and surface antigens. Research findings from the last decade suggest that EVs have an important, yet not completely understood, role in cell-cell communication. They can influence neighboring cells, act in an autocrine or paracrine manner, affect tumor growth, de novo-angiogenesis, and immunological functions. EVs are a novel and important tool to impact cell function and
STUDY QUESTION Are circulating microparticles (MPs) altered in young women with polycystic ovary syndrome (PCOS)? SUMMARY ANSWER Women with PCOS have elevated concentrations of circulating platelet-derived MPs, which exhibit increased annexin V binding and altered microRNA (miR) profiles compared with healthy volunteers. WHAT IS KNOWN ALREADY Some studies have shown that cardiovascular risk is increased in young women with PCOS but the mechanisms by which this occurs are uncertain. Circulating MPs are elevated in patients with cardiovascular disease but the characteristics of MPs in patients with PCOS are unclear. STUDY DESIGN, SIZE, DURATION Case-control study comprising 17 women with PCOS (mean ± SD; age 31 ± 7 years, BMI 29 ± 6 kg/m2) and 18 healthy volunteers (age 31 ± 6 years, BMI 30 ± 6 kg/m2). PARTICIPANTS/MATERIALS, SETTING, METHODS The study was conducted in a University hospital. Nanoparticle tracking analysis (NTA) and flow cytometry (CD41 platelet, CD11b monocyte, CD144 ...
Learn Basics of Extracellular Vesicles from Калифорнийский университет в Ирвайне. This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, ...
The duration of cisplatin release from most of the drug delivery devices seemed to be shorter than 14 days except large microparticles. The objective of this study was to fabricate and characterize cisplatin-loaded PLA microparticles, PLA/PLGA (30/70) composite microparticles, and fibers as formulations for long-term sustained delivery of cisplatin to treat C6 glioma in vitro by electrospray and electrospinning techniques. Cisplatin-loaded biodegradable microparticles with particle size of around 5 μm and fiber fabrics with diameter of 0.5-1.7 μm were obtained using electrospray and electrospinning techniques. Encapsulation efficiency and in vitro release of formulations were measured by ICP-OES. The encapsulation efficiency for different samples of microparticles was approximately from 33% to 72% and the fiber fabrics had encapsulation efficiency greater than 90%. Cisplatin-loaded microparticles showed typical characteristics of cisplatin release profile: a large initial burst followed by a ...
Recent studies suggest that extracellular vesicles may be the key to timely diagnosis and monitoring of genito-urological malignancies. In this study researchers investigated the composition and content of extracellular vesicles found in the urine of healthy donors and prostate cancer patients. Urine of 14 PCa patients
Macrophages are polarized into functional classically activated and alternatively activated (M2) phenotypes depending on their microenvironment, and these cells play an important role in the immune system. M2-like polarization of tumor-associated macrophages (TAMs) is activated by various secretions from cancer cells; however, the interaction between cancer cells and TAMs is not well understood. Recent studies showed that cancer cell-derived extracellular vesicles (EVs) contribute to tumor development and modulation of the tumor microenvironment. In the current study, we investigated colorectal cancer-derived EVs containing miR-145 with respect to the polarization of TAMs. Colorectal cancer cells positively secreted miR-145 via EVs, which were taken up by macrophage-like cells. Interestingly, colorectal cancer-derived EVs polarized macrophage-like cells into the M2-like phenotype through the downregulation of histone deacetylase 11. An in vivo study showed that EV-treated macrophages caused ...
Publikations-Datenbank der Fraunhofer Wissenschaftler und Institute: Aufsätze, Studien, Forschungsberichte, Konferenzbeiträge, Tagungsbände, Patente und Gebrauchsmuster
ThermoFisher Microparticles are manufactured in ISO-9001 certified, FDA-registered facilities following a computer-aided design. A wide range of microparticles of a variety of formats are available for use in diagnostics, research and industrial applications such as. ...
Mucopolysaccharidoses (MPS) are lysosomal storage disorders characterized by progressive accumulation of glycosaminoglycans (GAGs) due to deficiency of specific lysosomal enzymes. MPS are classified according to the enzyme ...
Extracellular vesicles (EVs) are specifically packed with nucleic acids, lipids, and proteins from their parental cell. subpopulation gun. In assessment to healthful plasma vesicles, most cancers plasma vesicles demonstrated modified indicators for platelet guns, suggesting buy NMS-1286937 a transformed vesicle release or proteins launching of EVs by platelets and a lower Compact disc8 transmission that might become connected with a reduced activity of NK cells or Capital t cells. As we barely recognized melanoma-derived vesicles in individuals plasma, we came to the conclusion that bloodstream cells caused the noticed variations. In overview, our outcomes query a immediate impact of most cancers cells on the structure of EVs in most cancers plasma, but rather claim for an roundabout impact of most cancers cells on the vesicle release or vesicle proteins launching by bloodstream cells. for 10?minutes (23). To deplete leukocytes and erythrocytes the platelet-rich plasma was centrifuged at ...
Extracellular vesicles (EVs), nanoparticles originated from different cell types, seem to be implicated in several cellular activities. In the Central Nervous System (CNS), glia and neurons secrete EVs and recent studies have demonstrated that the intercellular communication mediated by EVs has versatile functional impact in the cerebral homeostasis. This essential role may be due to their proteins and RNAs cargo that possibly modify the phenotypes of the targeted cells. Despite the increasing importance of EVs, little is known about their fluctuations in physiological as well as in pathological conditions. Furthermore, only few studies have investigated the contents of contemporary EVs subgroups (microvesicles, MVs and exosomes, EXOs) with the purpose of discriminating between their features and functional roles. In order to possibly shed light on these issues, we performed a pilot study in which MVs and EXOs extracted from serum samples of a little cohort of subjects (patients with the first clinical
Few studies have analyzed the effects of total circulating MPs, the physiological or pathophysiological real conditions, on angiogenesis. Recently, we have shown that circulating MPs from peroxisome proliferator-activated receptor-α wild-type mice, but not from knockout mice, induce the formation of capillary-like structures of endothelial cells. This effect, however, was abolished when the NF-κB pathway was inhibited, indicating the involvement of a mechanism dependent on NF-κB activation.50 Thus, peroxisome proliferator-activated receptor-α/NF-κB pathways activated by MPs may play a pivotal stimulatory role for angiogenesis of endothelial cells.. It has been shown that platelet MPs are able to modify steps involved in angiogenesis, such as proliferation, migration, and adhesion of endothelial cells. Kim et al53 were pioneers to demonstrate that platelet MPs increased proliferation, chemotatic migration, and formation of capillary-like tubes of human umbilical vein endothelial cells ...
We have developed an injectable foam suspension containing self-assembling, lipid-based microparticles encapsulating a core of pure oxygen gas for intravenous injection. Prototype suspensions were manufactured to contain between 50 and 90 ml of oxygen gas per deciliter of suspension. Particle size was polydisperse, with a mean particle diameter between 2 and 4 μm. When mixed with human blood ex vivo, oxygen transfer from 70 volume % microparticles was complete within 4 s. When the microparticles were infused by intravenous injection into hypoxemic rabbits, arterial saturations increased within seconds to near-normal levels; this was followed by a decrease in oxygen tensions after stopping the infusions. The particles were also infused into rabbits undergoing 15 min of complete tracheal occlusion. Oxygen microparticles significantly decreased the degree of hypoxemia in these rabbits, and the incidence of cardiac arrest and organ injury was reduced compared to controls. The ability to administer ...
Background/Aims: Proteinuria is associated with cardiovascular and chronic kidney disease. Microparticles (MPs) are bioactive vesicles shed from activated cells and also linked to cardiovascular disease. MP-like structures have been identified in the glomerular basement membrane, urinary space and between the glomerular basement membrane and the podocyte. We hypothesised that circulating MPs may provide a link between vascular injury and kidney diseases by inducing podocyte phenotypic alterations, thus propagating glomerular dysfunction and proteinuria. Methods:Human umbilical vein endothelial cells and U937 monocytes were stimulated with TNF-α to produce MPs. These MPs were confirmed by electron microscopy, and added to differentiated podocyte monolayers to determine effects on podocyte albumin endocytosis and the production of soluble mediators. Results:Monocyte and endothelial MPs upregulated podocyte production of pro-inflammatory mediators monocyte chemoattractant protein-1 (p , 0.001) and ...
Extracellular vesicles (EVs) such as exosomes and microvesicles are phospholipid bilayer-enclosed vesicles that are recognized as novel tools for intercellular communications and as biomarkers for several diseases. They contain various DNAs, proteins, mRNAs, and microRNAs (miRNAs) that have potential diagnostic and
Increasing attention has been given to secreted extracellular vesicles (EVs) in the past decades, especially in the portrayal of their molecular cargo and role as messengers in both homeostasis and pathophysiological conditions. This review presents the state-of-the-art proteomic technologies to identify and quantify EVs proteins along with their PTMs, interacting partners and structural details. The rapid growth of mass spectrometry-based analytical strategies for protein sequencing, PTMs and structural characterization has improved the level of molecular details that can be achieved from limited amount of EVs isolated from different biological sources. Here we will provide a perspective view on the achievements and challenges on EVs proteome characterization using mass spectrometry. A detailed bioinformatics approach will help us to picture the molecular fingerprint of EVs and understand better their pathophysiological function.
Introduction: Extracellular vesicles (EVs) are tiny membrane-enclosed vesicles released by cells by membrane budding or exocytosis. The molecular profile of contents and regenerative potential of EVs derived from murine induced pluripotent stem cells (miPSCs) have not been investigated.. Hypothesis: We hypothesized that miPSC-derived EVs carry bioactive substances, including mRNA and miRNA, which would influence the biology and fate of target cells.. Methods: Murine iPSCs were generated using established protocols and EVs were isolated from culture supernatants by sequential centrifugation. Atomic force microscopy (AFM), flow cytometry, and real-time RT-PCR were used to characterize EV contents. Endothelial cells were exposed to miPSC-EVs in culture.. Results: AFM and dynamic light scattering showed that miPSC-EVs are homogenous spherical vesicles smaller than 100 nm (Fig, A). High-sensitivity flow cytometry confirmed the presence of several iPSC- specific markers along with typical exosomal ...
Extracellular vesicles (EVs) produced by fungi are thought to influence pathogenesis and host responses to infection, rendering them potentially important in a medical context, particularly as targets for antivirulence drugs, and candidates for vaccines, for fungal diseases. Among other genera of fungi, EVs have been extracted from Candida, which are responsible for a large proportion of serious and fatal fungal disease cases. However, characterisation of EVs purportedly isolated from culture supernatants of Candida albicans may have resulted in erroneous conclusions being drawn regarding their physical attributes, functions and molecular cargo, due to a lack of purification of these structures from contaminants such as protein aggregates. This work aimed to investigate whether the ultracentrifugation-based extraction methods in previous studies were likely adequate enough to isolate C. albicans EVs from other entities, or whether additional purification procedures, namely size-exclusion ...
Extracellular Vesicles (EVs) are lipid vesicles secreted by cells that allow intercellular communication. They are decorated with surface proteins, which are membrane proteins that can be targeted by biochemical techniques to isolate EVs from background particles. EVs have recently attracted attention for their pot
Exosomes are nanometer-sized extracellular vesicles that are believed to function as intercellular communicators. and irradiated recipient cells. We found an enhanced uptake of exosomes isolated Pyronaridine Tetraphosphate from both irradiated and non-irradiated cells by irradiated recipient cells compared to nonirradiated recipient cells. Functional analyses by exosome transfer indicated that all exosomes (from non-irradiated and irradiated donor cells) increase the proliferation of non-irradiated recipient cells and the survival of irradiated recipient cells. The survival-promoting effects are more pronounced when exosomes isolated from irradiated compared to non-irradiated donor cells are transferred. A possible mechanism for the increased survival after irradiation could be the increase in DNA double-strand break repair monitored at 6 8 and 10 h after the transfer of exosomes isolated from irradiated cells. This is abrogated by the destabilization of the exosomes. Our results demonstrate ...
An efficient method of producing purified extracellular vesicles (EVs), in conjunction with a method that blocks liver macrophages from clearing EVs from the body, has produced promising results for the use of EVs in cancer therapy.
Extracellular vesicles (EVs), including exosomes and microvesicles, have emerged as promising drug delivery vehicles for small RNAs (siRNA and miRNA) due to their natural role in intercellular RNA...
Extracellular vesicles (EVs) are released by mammalian cells and are thought to be important mediators of intercellular communication. There are many methods for isolating EVs from cell culture...
Evidence suggests that extracellular vesicles (EVs) can play roles in physiology and pathology, providing impetus to explore their use as diagnostic and therapeutic targets
This article describes how extracellular vesicles are detected and the development of a robust and reproducible assay that overcomes previous limitations.
TY - JOUR. T1 - Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF V600E mutation AU - García-Silva, Susana. AU - Benito-Martín, Alberto. AU - Sánchez-Redondo, Sara. AU - Hernández-Barranco, Alberto. AU - Ximénez-Embún, Pilar. AU - Nogués, Laura. AU - Mazariegos, Marina S.. AU - Brinkmann, Kay. AU - López, Ana Amor. AU - Meyer, Lisa. AU - Rodríguez, Carlos. AU - García-Martín, Carmen. AU - Boskovic, Jasminka. AU - Letón, Rocío. AU - Montero, Cristina. AU - Robledo, Mercedes. AU - Santambrogio, Laura. AU - Brady, Mary Sue. AU - Szumera-Ciećkiewicz, Anna. AU - Kalinowska, Iwona. AU - Skog, Johan. AU - Noerholm, Mikkel. AU - Muñoz, Javier. AU - Ortiz-Romero, Pablo L.. AU - Ruano, Yolanda. AU - Rodríguez-Peralto, José L.. AU - Rutkowski, Piotr. AU - Peinado, Héctor. PY - 2019/5/1. Y1 - 2019/5/1. N2 - Liquid biopsies from cancer patients have the potential to improve diagnosis and prognosis. The assessment of surrogate markers ...
Hagiwara, K., Ochiya, T. and Kosaka, N. (2014) A Paradigm Shift for Extracellular Vesicles as Small RNA Carriers From Cellular Waste Elimination to Therapeutic Applications. Drug Delivery and Translational Research, 4, 31-37.
article{f64bcde2-c38f-4648-bf1a-09a66e77e807, abstract = {,p,Extracellular vesicles are cell-derived membrane particles ranging from 30 to 5,000 nm in size, including exosomes, microvesicles, and apoptotic bodies. They are released under physiological conditions, but also upon cellular activation, senescence, and apoptosis. They play an important role in intercellular communication. Their release may also maintain cellular integrity by ridding the cell of damaging substances. This review describes the biogenesis, uptake, and detection of extracellular vesicles in addition to the impact that they have on recipient cells, focusing on mechanisms important in the pathophysiology of kidney diseases, such as thrombosis, angiogenesis, tissue regeneration, immune modulation, and inflammation. In kidney diseases, extracellular vesicles may be utilized as biomarkers, as they are detected in both blood and urine. Furthermore, they may contribute to the pathophysiology of renal disease while also having ...
Microparticles (MPs) may be involved in the pathogenesis of systemic sclerosis (SSc), which includes vasculopathy, endothelial cell activation, and coagulation activation. Circulating MPs from SSc patients were characterized and their relationship with soluble markers of vascular activation investigated ...
Sculptra® Aesthetic works subtly and gradually over time for a more youthful-looking appearance. Unlike hyaluronic acid (HA) fillers, Sculptra Aesthetic helps stimulate your skins own natural collagen production to help restore its inner structure and increase facial volume that has been lost to aging.1. "Im impressed with the facial volume results of Sculptra," Dr. Randy Birken said. "I had it done and saw immediate results that have lasted over six months.". As a poly-L-lactic acid (PLLA) collagen stimulator, Sculptra is different. It works with your body within the deep dermis, rather than topically, to help revitalize collagen production and help restore your skins inner structure and volume.1 As the Sculptra Aesthetic PLLA microparticles are absorbed, they help stimulate collagen production. Rebuilt collagen strands begin gradually helping to restore facial volume and the look of fullness to wrinkles and folds.. For more information as well as before and after photos, go to ...
This invention provides high unit density arrays of microparticles and methods of assembling such arrays. The microparticles in the arrays may be functionalized with chemical or biological entities specific to a given target analyte. The high unit density arrays of this invention are formed on chips which may be combined to form multichip arrays according to the methods described herein. The chips and/or multichip arrays of this invention are useful for chemical and biological assays.
Vaccine formulations of synthetic peptides adsorbed to alum may not provide other requisite characteristics of an effective HIV vaccine, such as induction of mucosal immunity, production of cytotoxic T cells, and ease of administration. An oral microparticulate vaccine containing a prototype synthetic peptide has been developed. The microparticles can be degraded over time, inducing both secretory and systemic immune responses.. Twelve volunteers per dose regimen will receive oral microparticulate multivalent HIV-1 peptide vaccine at months 0, 1, and 6, either daily as a low dose for 3 days or a single higher dose. Additionally, four volunteers per regimen will receive placebo. Volunteers are followed for 1 year. They will be contacted once or twice yearly for 5 years to check on health status. ...
Corporate greed is allowed to flourish because the political establishment in Britain, and across much of the EU, is rotten. As always, Tory MPs are in bed with big business. But unfortunately, this is also the case with many of the Blairite MPs who remain within the Labour Party.. But the possibility of a break with this rotten system has been posed by Jeremy Corbyns leadership of the Labour Party.. Despite a number of significant retreats, Corbyn has made some good ground in recent weeks by promising free school dinners for children and the implementation of a £10 an hour living wage should Labour be elected in 2020 (or sooner).. He also supports the NHS Reinstatement Bill, which pledges to renationalise the health service - a policy proposal which Labours Shadow Health Secretary Jon Ashworth, somewhat perplexingly, refuses to support.. But Corbyn must go further than this. He must demand the nationalisation of large pharmaceutical companies, bringing them under democratic workers control ...
Mr. Speaker, the motion seeks to reinstate bills that died on the Order Paper when the previous session of Parliament ended.. As all of us know, the goal of the motion is a simple one: to spare members the burden of having to repeat work on bills that got as far as the committee stage in the last session.. This is especially commendable given the numerous pressures MPs are under and the limited resources available to us.. What features are contained in the motion? Simply put, under the motion a minister would be able to request during 30 sitting days after the motions adoption the reinstatement of a bill that had reached at least the committee stage when the last session ended. Should the Speaker be satisfied that the bill is the same as in the previous session, the bill would be reinstated at the same stage as before.. Thus during this session we can skip all the stages of debate that have been completed so far. The work of the committees that are considering the bills would consequently be ...
Exosomes and other extracellular vesicles (EVs) such as microvesicles carry functional cargo and play an important role in disease progression. Recently, exosomal RNAs, especially microRNAs, have attracted tremendous interest as potential circulating diagnostic biomarkers. This webinar presents an integrated system for identification and characterization of specific RNA molecules from exosomes and other extracellular vesicles (EVs). Advanced assay technologies for characterization of the RNA profiles including mRNA, microRNA and long non-coding RNAs (RNAs) from serum exosomes will be introduced ...
TY - JOUR. T1 - Microparticle surface layering through dry coating. T2 - Journal of Pharmacy and Pharmacology. AU - Alyami, Hamad. AU - Koner, Jasdip. AU - Dahmash, Eman Z.. AU - Bowen, James. AU - Terry, David. AU - Mohammed, Afzal R.. N1 - This is the peer reviewed version of the following article: Alyami, H., Koner, J., Dahmash, E. Z., Bowen, J., Terry, D., & Mohammed, A. R. (2016). Microparticle surface layering through dry coating: impact of moisture content and process parameters on the properties of orally disintegrating tablets. Journal of Pharmacy and Pharmacology, Early view, which has been published in final form at http://dx.doi.org/10.1111/jphp.12623. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.. PY - 2016/10/2. Y1 - 2016/10/2. N2 - OBJECTIVES: The aim of this study was to investigate the influence of process parameters during dry coating on particle and dosage form properties upon varying the surface adsorbed ...
Extracellular vesicles (EVs, including exosomes) are implicated in many aspects of nervous system development and function, including regulation of synaptic communication, synaptic strength, and nerve regeneration. They mediate the transfer of packets of information in the form of nonsecreted proteins and DNA/RNA protected within a membrane compartment. EVs are essential for the packaging and transport of many cell-fate proteins during development as well as many neurotoxic misfolded proteins during pathogenesis. This form of communication provides another dimension of cellular crosstalk, with the ability to assemble a "kit" of directional instructions made up of different molecular entities and address it to specific recipient cells. This multidimensional form of communication has special significance in the nervous system. How EVs help to orchestrate the wiring of the brain while allowing for plasticity associated with learning and memory and contribute to regeneration and degeneration are all ...
Several neural cell types in the brain, including neurons, glial cells, and oligodendrocytes, release EVs into the extracellular space and body fluids. EVs released from multiple types of neural cells not only contribute to intercellular communication between neural cells (e.g., neuron-glia interaction), synaptic plasticity, and neuronal activity, but are also responsible for the pathogenesis of neurological disorders [55]. Notably, several recent studies have suggested that EVs released from neural cells carry neuropathogenic molecules such as amyloid-beta (Aβ), α-syn, tau, superoxide dismutase 1, and TAR DNA-binding protein 43, which promote proteinopathic neurodegenerative diseases, as well as neuroprotective molecules against neuronal injury [2].. Parkinson disease: A hallmark of PD is the misfolding and aggregation of α-syn caused by failure of the ubiquitin-proteasome system or the autophagy-lysosome pathway. It has been reported that lysosomal inhibition increased the secretion of ...
Several neural cell types in the brain, including neurons, glial cells, and oligodendrocytes, release EVs into the extracellular space and body fluids. EVs released from multiple types of neural cells not only contribute to intercellular communication between neural cells (e.g., neuron-glia interaction), synaptic plasticity, and neuronal activity, but are also responsible for the pathogenesis of neurological disorders [55]. Notably, several recent studies have suggested that EVs released from neural cells carry neuropathogenic molecules such as amyloid-beta (Aβ), α-syn, tau, superoxide dismutase 1, and TAR DNA-binding protein 43, which promote proteinopathic neurodegenerative diseases, as well as neuroprotective molecules against neuronal injury [2].. Parkinson disease: A hallmark of PD is the misfolding and aggregation of α-syn caused by failure of the ubiquitin-proteasome system or the autophagy-lysosome pathway. It has been reported that lysosomal inhibition increased the secretion of ...
Journal of Cancer Metastasis and Treatment is an open access journal, focusing on basic and clinical studies related to cancer cell, cell biology, oncology, radiation therapy and radiology, obstetrics and gynecology, pediatrics, surgery, hematology, neuro-oncology, etc.
Unfortunately, in the PDF of the original version of this article [1] there was an error. The names of two of the co-first authors were included incorrectly in the footnote and read "Xiu-yang Huang" and "Juhong Yang "instead of "Xiu-yan Huang" and "Ju-hong Yang". The sentence should have read "Xiu-yan Huang, Zi-li Huang, and Ju-hong Yang are co-first authors".. The names have been also updated in the original article.. ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
(2013) Hardij et al. Journal of Extracellular Vesicles. INTRODUCTION: Extracellular vesicles (EVs) are shed from cells and carry markers of the parent cells. Vesicles derived from cancer cells reach the bloodstream and locally influence important physio...
View data on how to consistently obtain purely concentrated EVs with enough yield for subsequent proteomic, genomic, and transcriptomic analyses.
Introduction: PMP, EMP and LMP are known to be sensitive markers of thrombotic and inflammatory disorders, but their respective functional activities are obscure. Recent data indicate that these microparticles (MP) possess procoagulant, proaggregatory, and proinflammatory activities. In this study, we have compared these activities in MP derived from different cells.. Methods: PMP were prepared by stimulating platelet-rich plasma with 10 μM ADP plus 5 μg/mL collagen. EMP were prepared by incubating renal endothelial cells (EC) with 10 ng/mL of TNF-α for 24 hrs. LMP were prepared by incubating U937 monocytic cells or neutrophils with 10 ng/mL LPS for 1 hr. Cells were removed by centrifugation (1000xg for 10 min), MP were sedimented (15,000xg for 30 min), pellets were washed twice, then resuspended to equal MP concentration (1 x 108 counts/μL, final concentration) based on counts by flow cytometry. The MP were then tested for (ia) tissue factor (TF) antigen expression (TF:Ag) by flow ...
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Our overall objective is to develop and standardize methodologies, accelerating the identification and development of non-invasive biomarkers as well as define new strategies to tackle major diseases such as cancer and parasitic diseases. Thus, REDiEX signifies a significant advance towards personalized medicine aiming to develop new tools and markers with high social impact that will generate economic benefits and equity in global health.
As the incidence of osteoarthritis increased over the last years, development of new therapeutic approaches for osteochondral regeneration come into t
An improved chromatographic analytical column, the column containing a pellicular type (agglomerated) anion-exchange packing, comprising: Component A, which comprises a pressure packed bed of substrate particles of insoluble synthetic resin, having cation-exchanging sites at least on their available surfaces, the Component A particles being of low porosity relative to Component B microparticles, described below, and Component B, derived by agglomerating microparticles of insoluble synthetic resin onto the pressure packed bed of Component A particles, the microparticles having a volume average diameter of less than about 1,500 and greater than about 50 Angstroms and having anion-exchanging sites, at least on their outer surfaces, which attract available cation sites of Component A, wherein the microparticles of Component B are attached as a monolayer to the available surfaces of the Component A particles.
Polyethylene glycol monosubstituted with a polymerizable acrylic moiety was linked to 6-carboxy free position on dextran side chains and then subjected to radical polymerization with a comonomer in order to obtain microspheres for the oral controlled release of ketoconazole, a hydrophobic model drug. Microparticles were submitted to studies on their ability to absorb and retain water. Cell uptake studies, in the presence and absence of mucus, across two different monolayers, respectively, HT29-MTX-E12 and Caco-2, were done. Cytotoxicity studies were carried out to calculate the IC₅₀ value. The ability of microspheres to open monolayers tight junctions was tested by measuring their TEER values. Images of cell uptake were visualized by CLSM. In HT29-MTX-E12 cells, more mucoadhesion and drug internalization is seen thanks to the presence of PEG and dextran chains.. ...
Page contains details about example of encapsulated thermochromic material/light absorbing material/double-wall carbon nanotube composite microparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Page contains details about example of thermochromic material/light absorbing material/double-wall carbon nanotube composite microparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
A waterborne coating composition having a total solids content at application of greater than 30 percent, contains pigment and a dispersion of polymeric microparticles in an aqueous medium. The microparticles contain greater than 30 percent by weight based on the microparticles of a substantially hydrophobic polymer which is essentially free of repeating acrylic or vinyl units in the backbone, has a molecular weight greater than 300 and is adapted to be chemically bound into the cured coating composition. The aqueous medium of the microparticle dispersion is substantially free of water soluble polymer.
Summary Circulating procoagulant microparticles (MP) were measured as markers of vascular damage and prothrombotic risk in patients undergoing ST-segment myocardial infarction (STEMI) treated by primary percutaneous transluminal coronary angioplasty (PTCA) and additional GPIIb-IIIa antagonists. Cel...
TY - JOUR. T1 - Concentration-dependent protein loading of extracellular vesicles released by Histoplasma capsulatum after antibody treatment and its modulatory action upon macrophages. AU - Baltazar, Ludmila Matos. AU - Zamith-Miranda, Daniel. AU - Burnet, Meagan C.. AU - Choi, Hyungwon. AU - Nimrichter, Leonardo. AU - Nakayasu, Ernesto S.. AU - Nosanchuk, Joshua D.. PY - 2018/12/1. Y1 - 2018/12/1. N2 - Diverse pathogenic fungi secrete extracellular vesicles (EV) that contain macromolecules, including virulence factors that can modulate the host immune response. We recently demonstrated that the binding of monoclonal antibodies (mAb) modulates how Histoplasma capsulatum load and releases its extracellular vesicles (EV). In the present paper, we addressed a concentration-dependent impact on the fungus EV loading and release with different mAb, as well as the pathophysiological role of these EV during the host-pathogen interaction. We found that the mAbs differentially regulate EV content in ...
The effects of cocaine (150 nM, 300 nM, and 150 μM) on human glioblastoma cell cultures were studied on tunneling nanotube formation (1-h cocaine treatment) and extracellular vesicle release (1-, 3-, and 8-h cocaine treatment). Cocaine significantly increased the number of tunneling nanotubes only at the lowest concentration used. The release of extracellular vesicles (mainly exosomes) into the medium was stimulated by cocaine at each concentration used with a maximum effect at the highest concentration tested (150 μM). Moreover, cocaine (150 nM) significantly increased the number of vesicles with 61-80 nm diameter while at concentrations of 300 nM and 150 μM, and the smaller vesicles (30-40 nm diameter) were significantly increased with a reduction of the larger vesicles (41-60 nm diameter). A time dependence in the release of extracellular vesicles was observed. In view of the proposed role of these novel intercellular communication modes in the glial-neuronal plasticity, it seems possible ...
Inflammation and thrombosis are interrelated. For example, inflammation increases TF, platelet reactivity, fibrinogen, and leads to exposure of increased levels of phosphotidylserine, while decreasing TM and inhibiting fibrinolysis (by increasing PAI-1).4 We have used both a rat and mouse model of inferior vena cava (IVC) thrombosis in studies of the basic mechanisms of thrombogenesis and thrombus resolution. Cell adhesion molecules (CAMs) allow leukocyte transmigration, and selectins (P and E-selectin) are integrally involved in thrombosis. Selectins are the first upregulated glycoproteins on activated endothelial cells and platelets. The cell adhesion molecule P-selectin has been found upregulated in the vein wall as early as 6 h after thrombus induction, whereas E-selectin has been found upregulated at day 6 after thrombosis.5. Microparticles (MPs) are involved in the thrombotic process and the amplification of thrombosis. MPs are small (less than 1 micrometer, about the size of a bacterium), ...
Purpose : To investigate if topically applied mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) promote corneal wound repair in a rat alkali burn model Methods : EVs were isolated from media conditioned by human bone marrow-derived MSCs (n=4) using a series of centrifugation, filtration and ultracentrifugation steps followed by transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA; NanoSight) quality control. Pro-healing effects of hMSC-derived EVs (hMSC-EV) were examined in vitro by cell migration and scratch wound healing assays using human corneal epithelial (HCE) and endothelial (HCEC) cell lines. Human cornea wound healing rate was also determined ex vivo using fluorescein penetration test. Rat corneal alkali burn injury was induced using 1M NaOH and injury was treated with or without 30mg of hMSC-EVs. Efficacy of treatment was monitored by biomicroscopy and optical coherence tomography (OCT) followed by extensive tissue analysis at the end of the ...
The activation of mononuclear phagocytes (MPs), including monocytes, macrophages and dendritic cells, contribute to central nervous system inflammation in various neurological diseases. In HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP), MPs are reservoirs of HTLV-I, and induce proinflammatory cytokines and excess T cell responses. The virus-infected or activated MPs may play a role in immuneregulation and disease progression in patients with HTLV-I-associated neurological diseases. Results: Phenotypic analysis of CD14+ monocytes in HAM/TSP patients demonstrated high expression of CX3CR1 and HLA-DR in CD14lowCD16+ monocytes, compared to healthy normal donors (NDs) and asymptomatic carriers (ACs), and the production of TNF-alpha and IL-1beta in cultured CD14+ cells of HAM/TSP patients. CD14+ cells of HAM/TSP patients also showed acceleration of HTLV-I Tax expression in CD4+ T cells. Minocycline, an inhibitor of activated MPs, decreased TNF-alpha expression in CD14+ cells and ...
In vivo, thrombin serves as both the primary stimulus for thrombosis and subsequent thrombus growth on a template of activated platelets or platelet-derived procoagulant microparticles (15-17). Our group has shown that patients with clinically stable coronary artery disease (CAD) have evidence of increased thrombin activity and generation (18)as do those with unstable angina (19,20)and acute MI (21)in whom the abnormality persists for weeks to months after the presenting event (22). Consistent with prior observations (23), patients in this study exhibited increased thrombin generation despite treatment with UFH (10,18,24,25). The limitations associated with UFH administration may, at least in part, be the result of its relative inaccessibility to clot-bound thrombin and an inability to effectively neutralize factor Xa-mediated procoagulant activity (26).. The thrombogenicity of atheromatous plaques and dysfunctional endothelial cells is strongly influenced by tissue factor which is considered ...
The tremendous increase of coalbed methane (CBM) exploration and development occurring in the Powder River Basin (PRB) of Wyoming and Montana has tested the limit; of existing land use plans governing federal lands. The Bureau of Land Management (BLM), as land management agency within the U.S. Department of the Interior (Department), developed Resource Management Plans (RMPs) for the PRB some 10-15 years ago, prior to commercial-scale CBM development. BLM has recognized the need to update all 162 of its land use plans under the Federal Land Policy & Management Act of 1976 (FLPMA),1 and National Environmental Policy Act of 1969 (NEPA).2 Priority has been given to RMPs affecting the National Energy Policy and the BLM has recently undertaken environmental in pact statements (EISs) addressing CBM development within RMPs for both the Montana and Wyoming PRB.3 The Draft EIS and Draft Planning Amendment for the PRB Oil and Gas Project (WY-070-02-065) released in January 2002, proposes to amend the ...
"Macrophages discriminate glycosylation patterns of apoptotic cell-derived microparticles". J Biol Chem. 287 (1): 496-503. doi: ... Some white blood cells in human immune system perform phagocytosis by gulping in some pathogenic and disease causing cells. ... One of the features of an apoptotic cell is the presentation of a variety of intracellular molecules on the cell surface, such ... In cell biology, phagocytosis (from Ancient Greek φαγεῖν (phagein) , meaning 'to devour', κύτος, (kytos) , meaning 'cell', and ...
MIS416, a non-toxic microparticle adjuvant derived from Propionibacterium acnes comprising immunostimulatory muramyl dipeptide ... and Th17 cells in EAE, and to increase the serum levels of IFN-γ and IFN-γ-associated proteins in 19 patients with SPMS [97]. A ... "a myeloid-directed microparticle immune response modifier (derived from Propionibacterium acnes), which was originally ... a non-toxic microparticle adjuvant derived from Propionibacterium acnes comprising immunostimulatory muramyl dipeptide and ...
2004). "Detection of fetal DNA and RNA in placenta-derived syncytiotrophoblast microparticles generated in vitro". Clinical ... Cell-free fetal DNA (cffDNA) is fetal DNA which circulates freely in the maternal blood. Maternal blood is sampled by ... 2003). "Maternal serum cell-free fetal DNA levels are increased in cases of trisomy 13 but not trisomy 18". Human Genetics 112 ... Allyse, M.; Sayres, L. C.; King, J. S.; Norton, M. E.; Cho, M. K. (2012). "Cell-free fetal DNA testing for fetal aneuploidy and ...
Infused, negatively charged, immune-modifying microparticles (IMPs), derived from polystyrene, microdiamonds, or biodegradable ... This results in monocyte sequestration in the spleen through apoptotic cell clearance mechanisms. IMPs may therefore be of ... Cell. 80 (4): 603-9. doi:10.1016/0092-8674(95)90514-6. PMID 7867067. Kangas M, Brännström A, Elomaa O, Matsuda Y, Eddy R, Shows ... Immunology and Cell Biology. 94 (7): 646-55. doi:10.1038/icb.2016.20. PMID 26888252. Whelan FJ, Meehan CJ, Golding GB, McConkey ...
Caveolae Cellular dewetting Endothelial activation Endothelial microparticle Endothelial progenitor cell Endothelium-derived ... It is a thin layer of simple squamous cells called endothelial cells. Endothelial cells in direct contact with blood are called ... Vascular endothelial cells line the entire circulatory system, from the heart to the smallest capillaries. These cells have ... The foundational model of anatomy makes a distinction between endothelial cells and epithelial cells on the basis of which ...
Exosomes from red blood cells contain the transferrin receptor which is absent in mature erythrocytes. Dendritic cell-derived ... Chen, TS; Lai, RC; Lee, MM; Choo, AB; Lee, CN; Lim, SK (2010). "Mesenchymal stem cell secretes microparticles enriched in pre- ... By transferring molecules from one cell to another, exosomes from certain cells of the immune system, such as dendritic cells ... Exosomes are cell-derived vesicles that are present in many and perhaps all eukaryotic fluids, including blood, urine, and ...
In biological systems, microparticles are small membrane bound vesicles circulating in the blood derived from cells that are in ... Microparticles encountered in daily life include pollen, sand, dust, flour, and powdered sugar. Microparticles have a much ... For example, metal microparticles can be explosive in air. Microspheres are spherical microparticles, and are used where ... Microparticles are particles between 0.1 and 100 μ {\displaystyle \mu } m in size. Commercially available microparticles are ...
Martínez, MC; Tesse, A; Zobairi, F; Andriantsitohaina, R. Shed membrane microparticles from circulating and vascular cells in ... monocyte-derived and tissue factor-carrying circulating microparticles are related to acute myocardial infarction severity.. ... 胞漿顆粒(英语:Granule (cell biology)). 黑色素體 · 微粒體 · 過氧化物酶體 · 乙醛酸循環體 · 韋伯潘力氏小體(英语:Weibel-Palade body) ... A whole-cell electron tomography model of vacuole biogenesis in Arabidopsis root cells.. Nature plants
Agarose is a polysaccharide derived from seaweed used for nanoencapsulation of cells and the cell/agarose suspension can be ... Alginate microparticles loaded with myoblast cells and functionalized with RGD allowed control over the growth and ... autologous cells), from another donor (allogeneic cells) or from other species (xenogeneic cells). The use of autologous cells ... The cell type chosen for this technique depends on the desired application of the cell microcapsules. The cells put into the ...
... from cell microparticles to other particulated delivery systems (e.g. liposomes). Adjuvants are crucial in affecting the ... CSP and sporozoite surface protein 2 (called PfSSP2) were derived from the sporozoite phase. The liver stage antigen 1 (LSA1), ... The cell-mediated component consists of many white blood cells (such as monocytes, neutrophils, macrophages, lymphocytes, ... Waters A (February 2006). "Malaria: new vaccines for old?". Cell. 124 (4): 689-93. doi:10.1016/j.cell.2006.02.011. PMID ...
These cells, together with other immune cells such as macrophages, lymphocytes, neutrophils, mast cells, dendritic cells and ... The word "synovium" is derived from the word "synovia" (or synovial fluid), which is a clear, viscous fluid produced by the ... 2010). "Platelets Amplify Inflammation in Arthritis via Collagen-Dependent Microparticle Production". Science. 327 (5965): 580- ... Furthermore these cells express a number of molecules important for the mediation of the cell adhesion, such as cadherin-11, ...
DHA-derived Resolvins). These metabolites are formed by a wide range of cells and tissues by the initial metabolism of DHA to ... Dalli, Jesmond; Serhan, Charles N. (2012-10-11). "Specific lipid mediator signatures of human phagocytes: microparticles ... SPMs are locally formed and locally acting cell signaling autacoids: they are made by cells and act upon their parent or nearby ... often resides in a different cell type with one cell passing along the intermediate metabolite that it forms to the other cell ...
A theoretical expression for this spectrum is derived in the following, which can then be fitted to the experimental curve in ... Other applications are the rheology of soft matter, and studies of force-regulated processes in living cells. Forces are ... A magnetic tweezers apparatus consists of magnetic micro-particles, which can be manipulated with the help of an external ... In experiments, the molecule of interest is attached to a magnetic microparticle. The magnetic tweezer is equipped with magnets ...
Cancer cells may facilitate export of PAI-2 via microparticles.[19] PAI-2 provides protection for cancer cells against plasmin- ... "Cloning and expression of a cDNA coding for a human monocyte-derived plasminogen activator inhibitor". Proceedings of the ... Among immune cells, macrophages are the main producers of PAI-2, as both B-cells and T-cells do not produce significant amounts ... "Cell. 156 (5): 1002-16. doi:10.1016/j.cell.2014.01.040. PMC 3988473. PMID 24581498.. ...
Cross-priming occurs when a bone marrow-derived cell presents peptides from proteins synthesised in another cell in the context ... that has been absorbed onto gold or tungsten microparticles into the target cells, using compressed helium as an accelerant. ... Studies using chimeric mice have shown that antigen is presented by bone-marrow derived cells, which include dendritic cells, ... The type of T-cell help raised is determined by the differentiated state of antigen presenting cells. Dendritic cells can ...
... antimetastatic activity of dendritic cells based on vaccines when mechanically heterogenized microparticles of tumor cells are ... One such model, the patient derived tumour xenograft, has shown excellent utility in preserving tumour heterogeneity whilst ... The cancer stem cell model asserts that within a population of tumour cells, there is only a small subset of cells that are ... There are two models used to explain the heterogeneity of tumour cells. These are the cancer stem cell model and the clonal ...
Using light microscopy, Price discovered macrophage cells laden with micro-particles in lymph nodes of the affected extremity. ... the result of a genetically determined abnormal inflammatory reaction to mineral particles in irritant red clay soils derived ...
The suffix -on which is now used to designate other subatomic particles, such as a proton or neutron, is in turn derived from ... Electron therapy can treat such skin lesions as basal-cell carcinomas because an electron beam only penetrates to a limited ... who independently obtained the same result as Millikan using charged microparticles of metals, then published his results in ... Both electric and electricity are derived from the Latin ēlectrum (also the root of the alloy of the same name), which came ...
Many analysers are capable of measuring a "derived fibrinogen" level from the graph of the Prothrombin time clot. If a ... Hoffman, M; Monroe DM, 3rd (June 2001). "A cell-based model of hemostasis" (PDF). Thrombosis and haemostasis. 85 (6): 958-65. ... as well as procoagulant microparticles or microvesicles shed from them. Calcium is also required at other points in the ... Protein C is activated in a sequence that starts with Protein C and thrombin binding to a cell surface protein thrombomodulin. ...
Secondary cells and batteries containing alkaline or other non-acid electrolytes - Secondary lithium cells and batteries for ... Qi, Zhaoxiang; Koenig, Gary M. (16 August 2016). "High-Performance LiCoO2Sub-Micrometer Materials from Scalable Microparticle ... "Layered Lithium-Manganese Oxide Electrodes Derived from Rock-Salt LixMnyOz (x+y=z) Precursors" 194th Meeting of the ... Battery versus cellEdit. A cell is a basic electrochemical unit that contains the electrodes, separator, and electrolyte.[19][ ...
... s have no cell nucleus: they are fragments of cytoplasm that are derived from the megakaryocytes[2] of the bone marrow ... "Platelets amplify inflammation in arthritis via collagen-dependent microparticle production". Science. 327 (5965): 580-3. ... Berridge, Michael J. (1 October 2014). "Module 11: Cell Stress, Inflammatory Responses and Cell Death". Cell Signalling Biology ... "Programmed anuclear cell death delimits platelet life span". Cell. 128 (6): 1173-86. doi:10.1016/j.cell.2007.01.037. PMID ...
Extensive studies in Europe indicate that the chlorine found in emitted dioxins is not derived from HCl in the flue gases. ... Thus laden with high levels of POPs, the microparticles are often ingested by organisms in the biosphere. However, there is ... see Closed-cell PVC foamboard). Sheets are cut using saws and rotary cutting equipment. Plasticized PVC is also used to produce ... Weathering degradation of plastics results in their surface embrittlement and microcracking, yielding microparticles that ...
Electrochemical cell - Electrodynamics - Electrolytic cell - Electromagnet - A type of magnet in which the magnetic field is ... Contents: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z See also References External links Joule - A derived unit of ... Angle of incidence - Angle of reflection - Angstrom - Its a unit of linear measurement for measuring micro-particles. It's ... Contents: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z See also References External links Watt - A derived unit of ...
Syntehtic peptides entrapped in microparticles can elicit cytotoxic T cell T cell activity. Vaccine 1996; 14:1523. 20. Hioe CE ... This eventual result freed up the valuable future information to be derived from detailed RNA or DNA sequences for the benefit ... Stimulation and expansion of a human T cell subpopulation by a monoclonal antibody to T cell receptor T cell receptor molecule ... An antigen characteristic of hairy cell leukemia cells is expressed on certain activated B cells. J immunol 1984; 133:1635. 48 ...
Mediates cell adhesion. Glomerulopathy with fibronectin deposits. antithrombin III. Inhibits IIa, Xa, and other proteases. ... Many analysers are capable of measuring a "derived fibrinogen" level from the graph of the Prothrombin time clot. ... as well as procoagulant microparticles or microvesicles shed from them. Calcium is also required at other points in the ... Protein C is activated in a sequence that starts with Protein C and thrombin binding to a cell surface protein thrombomodulin. ...
In the cells of a typical tissue, the partial pressure of oxygen will drop, while the partial pressure of carbon dioxide will ... The term "decompression" derives from the reduction in ambient pressure experienced by the organism and refers to both the ... This damage may release endothelial membrane microparticles. If a bubble or an object exists which collects gas molecules this ... As the bubble grows it may distort the surrounding tissue and cause damage to cells and pressure on nerves resulting in pain, ...
positive regulation of blood microparticle formation. • negative regulation of endothelial cell proliferation. • positive ... As most information regarding TNF signaling is derived from TNFR1, the role of TNFR2 is likely underestimated. ... but it is produced also by a broad variety of cell types including lymphoid cells, mast cells, endothelial cells, cardiac ... regulation of heterotypic cell-cell adhesion. • negative regulation of mitotic cell cycle. • endothelial cell apoptotic process ...
Endothelial progenitor cells (EPCs) are cells derived from the bone marrow which differentiate into endothelial cells to help ... Endothelial Progenitor Cells, and Endothelial Microparticles in Cancer. Neoplasia. 8: 79-88 Goon, P.K.Y, Lip, G.Y.H., Boos, C.J ... Stonelake, P.S., Blann, A.D. (2006) Circulating Endothelial Cells, Endothelial Progenitor Cells, and Endothelial Microparticles ... Circulating endothelial cells (CECs) are endothelial cells that have been shed from the lining of the vascular wall into the ...
It has been known that levels of circulating cell-derived microparticles (MP) are generally increased in inflammatory states, ...
Normally, inhaled microparticles are cleaned off by alveolar macrophages via phagocytosis. However, this is not applicable to ... Traffic-derived nanosized particles are most likely responsible for these cardiovascular actions, because the larger surface ... Applications of Advanced Cell Culture in Cell Physiology: Co-culture, Organoids, Stem Cells: Submission deadline December 31, ... Cells from passages 4 and 5 were used. Treatment was started soon after splitting cells from passages 3 and 4. Culture media ...
Cell-derived microparticles (MPs) have been described as vital contributors to the inflammatory process. However, its role in ... Keywords : chronic periodontitis; diabetes; gingival crevicular fluid; cell-derived microparticles. · abstract in Portuguese · ... Cell Derived Microparticles in Gingival Crevicular Fluid from Periodontitis Patients with Type 2 Diabetes. Braz. Dent. J. [ ... Therefore, we aimed to detect the presence neutrophil (CD66b+) and platelet (CD41b+) derived microparticles in gingival ...
A Useful Biomarker for Predicting One-Year Mortality in Patients with Advanced Non-Small Cell Lung Cancer. Chin-Chou Wang,1,2,3 ... platelet-derived apoptotic MPs (PDAp-MPs), (3) endothelial-derived activated MPs (EDAc-MPs), and (4) endothelial-derived ... This study tested the hypothesis that circulating microparticles (MPs) are useful biomarkers for predicting one-year mortality ... Levels of four MPs in circulation, (1) platelet-derived activated MPs (PDAc-MPs), (2) ...
... recently reported to associate with elevated microvesicle/microparticle (MP) counts, have also long been known to be ... Detection and Isolation of Cell-Derived Microparticles Are Compromised by Protein Complexes Resulting From Shared Biophysical ... These data may necessitate reevaluation of certain published data on patient-derived MPs and contribute to correct the clinical ... Numerous diseases, recently reported to associate with elevated microvesicle/microparticle (MP) counts, have also long been ...
The Impact of Sickle Cell Disease Induced Oxidative Stress on MP Proteome ... Analysis of RBC Derived Microparticles from Sickle Cell Mice using Mass Spectrometry: ... Analysis of RBC Derived Microparticles from Sickle Cell Mice using Mass Spectrometry: The Impact of Sickle Cell Disease Induced ... This seminar, will also focus on proteomic differences between Microparticles derived from wildtype and SCD transgenic mouse ...
... derived from kidney-derived mesenchymal stem cells (KMSCs) have recently been reported to ameliorate rarefaction of peritubular ... In vivo administration of KMSC-derived MPs significantly inhibited EndoMT of PTC endothelial cells and improved PTC rarefaction ... Furthermore, administration of KMSC-derived MPs inhibited inflammatory cell infiltration as well as tubulointerstitial fibrosis ... KMSC-derived MPs significantly ameliorated EndoMT and improved in vitro proliferation of TGF-β1 treated HUVEC. ...
... and for adhesive cells including adipose-derived stromal cells (ADSCs) and bone marrow-derived mesenchymal stem cells (BMSCs). ... Furthermore, LMWH/P N/MPs can also bind to adhesive cell surfaces, inducing cells and LMWH/P N/MPs-aggregate formation. Those ... cultures of cells including ADSCs and BMSCs using plasma-medium gel with LMWH/P N/MPs exhibited efficient cell proliferation. ... LMWH/P N/MPs can also bind to tissue culture plates and adsorb exogenous GFs or GFs from those cells. The LMWH/P N/MPs-coated ...
CONCLUSION(S) Cell-derived microparticles were raised in normal pregnancy. Recurrent miscarriage seems to be related to ... endothelial and platelet cell activation and/or consumption. An increase in almost all cMP types was observed in PE. A ... OBJECTIVE To assess current studies on the relationship between cell-derived microparticles (cMP) and recurrent miscarriages ( ... Circulating cell-derived microparticles in severe preeclampsia and in fetal growth restriction.. Jaume Alijotas-Reig, Carles ...
Cell-Derived Microparticles Promote Coagulation after Moderate Exercise. SOSSDORF, MAIK; OTTO, GORDON P.; CLAUS, RALF A.; More ...
Improving the dissolution of fenofibrate with yeast cell-derived hollow core/shell carbon microparticles. In: RSC Advances. ... Improving the dissolution of fenofibrate with yeast cell-derived hollow core/shell carbon microparticles. RSC Advances. 2016 ... Improving the dissolution of fenofibrate with yeast cell-derived hollow core/shell carbon microparticles. / Huang, Xiaoxi; ... title = "Improving the dissolution of fenofibrate with yeast cell-derived hollow core/shell carbon microparticles", ...
Circulating Endothelium-Derived Microparticles (EMPs). Preparation of platelet-free plasma. Endothelium-derived microparticles ... endothelial microparticle. EPC. endothelial progenitor cell. FMD. flow-mediated dilation. L-NMMA. LG-monomethyl-arginine. NO. ... 2003) Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis. Thromb ... 2003) Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis. Thromb ...
Cell-derived Microparticles. Extracellular membrane vesicles generated by the shedding of CELL MEMBRANES blebs. Microparticles ... Membrane microparticles are submicron fragments of membrane vesicles shed from various cell types. Circulating endothelial ... Microparticles are used in a variety of different fields, such as drug delivery. Recently, non-spherical microparticle ... and other cell types circulate in the peripheral blood and through the MICROVASCULATURE where larger cells cannot, functioning ...
... monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T ... are excellent vehicles for releasing molecules inside the cell. The aim of this work was to use MSMPs to deliver viral specific ... MHC class I restricted epitopes into human antigen presenting cells ( ... The microparticles are internalized into dendritic cells (DC). (a) Photographs show control cells (MDDCs) or with internalized ...
Neutrophil microparticles-induced injuries and morphological alterations in human umbilical vein endothelial cells (HUVECs) ... In this study we investigated the potential role of myeloperoxidase in the endothelial cell injury caused by neutrophil-derived ... Exposure of HUVEC to neutrophil microparticles induced a loss of cell membrane integrity and morphological changes. The ... in neutrophil microparticles and that the microparticle-associated myeloperoxidase cause injury to endothelial cells. Hence, ...
Cell-cell communication has proven to be even more complex than previously thought since the discovery that extracellular ... Cell-derived microparticles. *Exosomes. *Microrna. Get free article suggestions today. Mendeley saves you time finding and ... Cell-cell communication has proven to be even more complex than previously thought since the discovery that extracellular ... More than simply cell dust, extracellular vesicles are capable of transferring biological information to neighboring cells and ...
Cell-Derived Microparticles in Patients with Type 2 Diabetes Mellitus: a Systematic Review and Meta-Analysis ... Type 2 diabetes mellitus Microparticles Biomarker Platelet-derived microparticle Endothelium-derived microparticle ... Supplementary Material for: Cell-Derived Microparticles in Patients with Type 2 Diabetes Mellitus: a Systematic Review and Meta ... i,Background/Aims: ,/i,The aim of this study was to assess the association between circulating cell-derived microparticles (MPs ...
Cell-derived microparticles and the lung.. Nieri D, Neri T, Petrini S, Vagaggini B, Paggiaro P, Celi A. ... Tiotropium inhibits proinflammatory microparticle generation by human bronchial and endothelial cells.. Neri T, Scalise V, ... Leptin induces the generation of procoagulant, tissue factor bearing microparticles by human peripheral blood mononuclear cells ... Pirfenidone inhibits p38-mediated generation of procoagulant microparticles by human alveolar epithelial cells. ...
Washing samples results in decreased AnnV+MPs (P=0.002) and platelet-derived MPs (PMPs) (P=0.002). Double centrifugation of MPs ... Circulating cell-derived microparticles (MPs) have been implicated in several disease processes and elevated levels are found ... Cell-Derived Microparticles, Endothelial Cells, Flow Cytometry, Humans, Leukocytes, Middle Aged, Platelet Endothelial Cell ... Measurement of circulating cell-derived microparticles by flow cytometry: sources of variability within the assay. ...
It is well known that danger-associated molecular patterns (DAMPs) can activate immune cells, including dendritic cells (DCs), ... The supernatants of dying CRC cells treated with OXA/5-Fu promoted mouse and human DC maturation, with upregulation of HLA-DR, ... Here, we found that colorectal cancer (CRC) cells treated with oxaliplatin (OXA) and/or 5-fluorouracil (5-Fu) released high ... Vaccines composed of DCs pulsed with the supernatants of chemically stressed CRC cells induced a more significant IFN-γ- ...
A Cell Culture Model of Resistance Arteries , Isolation and Characterization of Neutrophil-derived Microparticles for ... Using Human Induced Pluripotent Stem Cell-Derived Hepatocyte-Like Cells for Drug Discovery, Flow-pattern Guided Fabrication ... Single-cell Microinjection for Cell Communication Analysis, Mechanical Stimulation-induced Calcium Wave Propagation in Cell ... Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells, Phage- ...
Levels of circulating EPC-microparticles (MPs) and endothelium-derived MPs (EMPs) in db/db mice are changed in basal and after ... A and B: the P1 gate for CD45+ cells. C-F: EPCs are defined as CD34+/VEGFR+ (top right) cells gated on P1 gate (C: db/+ sham; D ... Circulating endothelial progenitor cells and cellular membrane microparticles in db/db diabetic mouse: possible implications in ... Circulating endothelial progenitor cells and cellular membrane microparticles in db/db diabetic mouse: possible implications in ...
Thus, microparticles can transfer chemokine receptors and arachidonic acid between cells, activate complement, promote ... A role in inflammatory arthritis is suggested by the finding that leukocyte-derived microparticles induce the production of ... Under certain conditions, however, microparticles may also exert anti-inflammatory properties by inducing immune cell apoptosis ... Microparticles may play an important role in the pathogenesis of rheumatologic diseases as evidenced by their elevation in ...
Cell-Derived Microparticles/Exosomes in Neuroinflammation Lawrence L. Horstman, Wenche Jy, Yeon S. Ahn ... Emerging Roles of Endothelial Cells in Multiple Sclerosis Pathophysiology and Therapy J. Winny Yun, Alireza Minagar, J. Steven ... Inflammation disorders Neuroinflammation Immune system Endothelial cells Multiple sclerosis Neurosarcoidosis Guillain Barre ... cerebral endothelial cells, and other main components of the immune cascade. Cutting-edge and authoritative, this volume offers ...
  • abstract = "Hollow core/shell carbon microparticles, denoted HCSC600, are synthesized from yeast cells by coating the cells with silica shells, and then pyrolyzing the silica-protected yeast cells at 600°C, and finally etching the silica shells off of the carbonized products. (elsevier.com)
  • Hyperglycemic-derived EMPs induced significantly higher surface expression of E-selectin (2614 ± 132 vs. 2010 ± 204 MFI), ICAM-1 (2110 ± 81 vs. 1688 ± 152 MFI), VCAM-1 (3589 ± 431 vs. 2134 ± 386) and PECAM-1 (4237 ± 395 vs. 2525 ± 269 MFI) on endothelial cells than EMPs from normoglycemic conditions. (springermedizin.de)
  • however, the role of DAMPs released from chemical drug-treated tumor cells in the activation of the immune response needs to be further elucidated. (nature.com)
  • Collectively, these results demonstrate that DAMPs released from chemically stressed cancer cells can activate DCs via TLR4 and enhance the induction of an anti-tumor T-cell immune response, delineating a clinically relevant immuno-adjuvant pathway triggered by DAMPs. (nature.com)
  • Efficiently killing tumor cells with chemical drugs while enhancing induction of the anti-tumor immune response is becoming increasingly important. (nature.com)
  • CRC cells treated with OXA and 5-Fu may release danger-associated molecular patterns (DAMPs), which play important roles in immunogenic tumor cell death. (nature.com)
  • However, tumor-derived factors maintain DC immaturity, trapping the cells within the tumor and hence impairing effective presentation of ingested antigens to T cells. (nature.com)
  • On the other hand, tumor and stem cell derived EVs have been identified to exert an inhibitory effect on immune responses by carrying immuno-modulatory effectors, such as transcriptional factors, non-coding RNA (Species), and cytokines. (frontiersin.org)
  • Increasing evidence indicates that tumor endothelial cells (TEC) differ from normal endothelial cells (NEC). (plos.org)
  • Recent studies have shown that TMV contain numerous types of bioactive molecules and affect normal stromal cells in the tumor microenvironment. (plos.org)
  • Here we showed that TMV isolated from tumor cells were taken up by NEC through endocytosis. (plos.org)
  • These findings offer new insights into cancer therapies and the crosstalk between tumor and endothelial cells mediated by TMV in the tumor microenvironment. (plos.org)
  • Tumor blood vessels provide nutrition and oxygen, promote tumor progression, and serve as gatekeepers for tumor cells to metastasize to other organs. (plos.org)
  • Furthermore, there are many differences at the molecular and functional levels between tumor endothelial cells (TEC) and normal EC (NEC) , . (plos.org)
  • MVs are released from various tumor cells and some progenitors of differentiated cells . (plos.org)
  • Tumor-derived MVs (TMV) contain bioactive molecules such as microRNAs (miRNA), mRNAs, and/or proteins inside themselves . (plos.org)
  • Stem cells (SCs) could transport therapeutic cargoes to tumor microenvironment via their intrinsic tumor-tropic properties[ 24 ]. (thno.org)
  • 3 Others have shown that intravenous injection of some tumor cells may cause significant thrombocytopenia (50% to 70%) in mice. (ahajournals.org)
  • Using human plasma samples, tumor tissue, cancer cell lines, and genetically engineered mouse models, the origins of these proteins are being investigated. (stanford.edu)
  • However, CXCL4L1 is also expressed in patient tumors, tumor cell lines, and murine xenografts, prompting a more detailed analysis of its role in cancer pathogenesis. (aacrjournals.org)
  • We found that myofibroblasts triggered cues in the tumor microenvironment, which led to induction of CXCL4L1 in tumor cells. (aacrjournals.org)
  • CXCL4L1 inhibited angiogenesis but also affected tumor development more directly, depending on the tumor cell type. (aacrjournals.org)
  • Furthermore, inhibition of tumor development was observed in tumors derived from implantation of A549, LLC, and B16 cells in mice, treated with recombinant CXCL4L1 ( 1 ). (aacrjournals.org)
  • The cell secretome produced by human adipose derived stem cells (ASCs) under the stimulation of tumor necrosis factor-α (TNF-α), when applied to rat excisional wound model, demonstrated accelerated wound closure, angiogenesis, and increased level of macrophage infiltration into the cutaneous wound as compared to the control groups 8 . (thno.org)
  • Human tumor cells stained for DAPI (blue), cytochrome c (red), and CHCHD4 (green). (jci.org)
  • RB1 was the first identified tumor suppressor gene and has defined roles in the regulation of cell cycle progression, DNA replication, and terminal differentiation. (jci.org)
  • In conclusion, this study reported the presence of neutrophil and platelet derived microparticles in gingival crevicular fluid from individuals having severe periodontitis and type 2 diabetes. (scielo.br)
  • It involves virtually all renal intrinsic and infiltrating cells and is characterized by alterations in their phenotype and accumulation of excessive extracellular matrix proteins [ 1 - 4 ]. (biomedcentral.com)
  • Upon activation neutrophil releases microparticles - small plasma membrane vesicles that contain cell surface proteins and cytoplasmic matter, with biological activities. (biomedcentral.com)
  • The encapsulation of recombinant proteins in biocompatible and biodegradable nano- and microparticles is emerging as a promising approach to boost their immunogenicity by passively targeting them to antigen presenting cells (APCs) [ 7 - 9 ]. (hindawi.com)
  • This emerging field pertains to nano & microparticles that carry proteins and RNA, both of which serve important cellular functions, and is likely to be an important player in the fast evolving field of nanomedicine. (ispub.com)
  • For example, carrier erythrocytes (red blood cells, RBCs) were widely used to encapsulate or bind small-molecule agents, nucleic acids, proteins, and NPs to treat systemic disease owing to their long lifespan and high biocompatibility[ 16 , 22 , 23 ]. (thno.org)
  • Platelet microparticles are small in size (0.05 to 1 μm) with a defined plasma membrane and express selected platelet membrane and cellular proteins. (ahajournals.org)
  • This laboratory is also focused on the identification of proteins with expression restricted to the surface of cancer cells which can be used as novel targets for molecular imaging technologies. (stanford.edu)
  • In this work, we analyzed the global profile of proteins secreted by umbilical cord matrix (UCM) derived-MSC in static conditions by using mass spectrometry, enabling the identification of thousands of proteins. (stanford.edu)
  • The cell secretome represents the collection of proteins that are secreted and released from living cells 1 - 3 . (thno.org)
  • Examples of secreted proteins in the cell secretome include growth factors, immunoregulatory cytokines, extracellular matrix-degrading enzymes, and cell motility factors 4 - 6 . (thno.org)
  • The ability to identify and quantify the specific proteins in cell secretome that are responsible for the therapeutic outcomes after cell secretome treatment will lead to the development of more effective strategies for disease treatment. (thno.org)
  • I]n a 2015 phase Ib/IIa clinical trial, MIS416 was shown to suppress the development of proinflammatory T helper (Th)1, Th2, and Th17 cells in EAE, and to increase the serum levels of IFN-γ and IFN-γ-associated proteins in 19 patients with SPMS . (wikipedia.org)
  • Resuscitated patients show substantially increased levels of different (annexin V+) microparticles and their conjugates immediately and 24 hours after cardiopulmonary resuscitation, suggesting an early onset of inflammation, an ongoing endothelial activation and a procoagulatory state. (biomedcentral.com)
  • One of the features of an apoptotic cell is the presentation of a variety of intracellular molecules on the cell surface, such as calreticulin, phosphatidylserine (from the inner layer of the plasma membrane), annexin A1, oxidised LDL and altered glycans. (wikipedia.org)
  • The development of cell-based therapy for clinical studies raises the question whether the application of cell-based products to humans is safe. (selectbiosciences.com)
  • Prostacyclin, derived by the action of the cyclooxygenase system, is another endothelium-derived vasodilator that acts independently of NO. 11 Although it may contribute to some of the other regulatory roles of the endothelium, it appears to have a more limited role in the maintenance of vasodilator tone in humans. (ahajournals.org)
  • In humans, the amoebozoan entamoeba histolytica can phagocytose red blood cells. (wikipedia.org)
  • Rapid isolation of two distinct plasma membrane microparticle populations for biomarker discovery and assay. (ispub.com)
  • The methods of isolation, downstream quantification, and culture conditions of the EV producing cells have been discussed. (pubmedcentralcanada.ca)
  • In conclusion, various factors may impact the resulting EV yield, including technical aspects such as EV isolation and quantification procedures, and biological aspects such as cell type and culture conditions. (pubmedcentralcanada.ca)
  • Detection and isolation of cell-derived microparticles are compromised by protein complexes due to shared biophysical parameters. (uzh.ch)
  • The growth of myeloma cells is also modulated by cytokines or stromal bone growth factors, such as interleukin 6 (IL-6), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), insulin-like growth factor 1 (IGF-1), transforming growth factor-beta (TGF-β) and other factors that interfere with bone structure. (jcancer.org)
  • The vascular endothelial growth factor (VEGF)-165 protein was tested as a model cell secretome protein in the serum and serum-free conditions. (thno.org)
  • MSCs, representing approximately 0.001% of all nucleated cells in bone marrow, are a heterogeneous population of adult stem cells with a high proliferative potential and the ability to differentiate. (jcancer.org)
  • Stem/progenitor cell-derived EVs have been identified to exert immunomodulatory effects on target cells through transferring protein molecules as well as regulatory effects on the phenotype of target cells through fusion with the target cells membrane and/or through direct endocytosis by target cells to transfer nucleic acid substances (such as mRNA, miRNA) to the target cells. (springer.com)
  • TF-A mRNA expression has been detected in several cancer cell lines as well as in human endothelial cells (5, 11). (thefreelibrary.com)
  • The activity of the myeloperoxidase present on the microparticles was confirmed by hypochlorous acid detection. (biomedcentral.com)
  • Methods We collected sera from patients with SLE (n=64), patients with other autoimmune diseases (n=31) and healthy controls (n=35), and assayed them using a cell-based reporter system that enables highly sensitive detection of IFN-I and ISG-inducing activity. (bmj.com)
  • A third project focuses on the detection and function of microparticles in the development and propogation of vascular disease and cancer. (case.edu)
  • We evaluated the feasibility of our developed immuno-disaggregation bioassays using antibody-conjugated MPs and protein-conjugated MPs for the detection of target cell secretome protein. (thno.org)
  • 4 - 10 Among all, autologous bone marrow-derived CD34 + stem cells demonstrate a major benefit, showing favorable impact on angiogenic growth, without the toxicity and inflammatory response observed in nonautologous cell-based therapy. (ahajournals.org)
  • In this regard, overexpressing angiogenic growth factors in transplanted cells has been reported to be beneficial by attenuating myocardial ischemia and preserving the heart function. (ahajournals.org)
  • In this issue of Circulation Research , Mackie et al investigate the mechanisms of preserving the heart function after transplantation of CD34 + cells overexpressing sonic hedgehog (Shh) protein, a well-established angiogenic factor. (ahajournals.org)
  • 23 , 24 Notably, the vasoregenerative and angiogenic potential of angiogenic early outgrowth cells can be enhanced by the transfer of the CXCL12 receptor CXCR4 through platelet microparticles. (ahajournals.org)
  • Microparticle content of platelet concentrates is predicted by donor microparticles and is altered by production methods and stress. (semanticscholar.org)
  • The microparticles are characterized by various methods, including scanning electron microscopy, transmission electron microscopy, nitrogen gas adsorption/desorption and X-ray photoelectron spectroscopy. (elsevier.com)
  • Discrepancy in literature may be due to stem cell culture conditions, methods of EV purification, EV molecular content, and functional state of both parental and target cells. (frontiersin.org)
  • However, they also highlight the need for new, more sensitive methods for measuring cell-derived vesicles. (isac-net.org)
  • 2008 ). By harvesting body fluids or cell culture supernatants, EVs can be isolated with a wide range of different methods, such as ultracentrifugation (UC) (Van Deun et al. (pubmedcentralcanada.ca)
  • The pioneering experiments of Furchgott and Zawadzki first demonstrated an endothelium-derived relaxing factor that was subsequently shown to be nitric oxide (NO). 5 NO is generated from L-arginine by the action of endothelial NO synthase (eNOS) in the presence of cofactors such as tetrahydrobiopterin. (ahajournals.org)
  • 9 The endothelium-derived hyperpolarizing factors involved in this process are only partially understood (such as the cytochrome-derived factors and possibly C-type natriuretic peptide), and may differ between vascular beds. (ahajournals.org)
  • However, it is well recognized that Endothelium-Derived Hyperpolarizing Factor can compensate for loss of NO-mediated vasodilator tone, particularly in the microcirculation, and this appears important when NO bioavailability is reduced. (ahajournals.org)
  • This is characterized by a reduced bioavailability of endothelium-derived vasodilators, such as nitric oxide (NO), along with a relative or absolute increase in available vasoconstrictors. (scielo.br)
  • The aim of this work was to use MSMPs to deliver viral specific MHC class I restricted epitopes into human antigen presenting cells (monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T lymphocytes. (hindawi.com)
  • In this study we identify LAMP-1 (CD107a) and LAMP-2 (CD107b) on the surface of human monocyte-derived dendritic cells (MoDC) and show only LAMP-2 is internalized after ligation by specific Abs, including H4B4, and traffics rapidly but transiently to the MHC class II loading compartment, as does Ag conjugated to H4B4. (jimmunol.org)
  • Monocyte-derived microparticles (MMPs) contain organized membrane receptors including ß2-integrins, like Mac-1 (CD11b/CD18). (biomedcentral.com)
  • Patients with this disorder have antibodies reactive with a plasma protein, beta2-glycoprotein 1, and we have shown that these antibodies activate endothelial cells in a beta2-GPI dependent manner. (case.edu)
  • 18 After inducing myocardial infarction in nude mice, the authors transplanted the Shh-modified CD34 (CD34 Shh ) cells, which significantly preserved cardiac function, reduced infarct size, and increased angiogenesis within the infarct border zone, as compared to the empty vector-transfected or -untransfected CD34 + cells. (ahajournals.org)
  • 10,11 With the exception of platelet-derived sphingosine-1-phosphate, which stimulates endothelial cell migration and angiogenesis by upregulating the expression of adhesion molecules, 12 the majority of research in this area has focused on platelet secretion of protein factors. (ahajournals.org)
  • 2 kDa) that are capable of stimulating endothelial cell migration, a key component of angiogenesis. (ahajournals.org)
  • Plasmodium vivax merozoites only invade reticulocytes, a minor though heterogeneous population of red blood cell precursors that can be graded by levels of transferrin receptor (CD71) expression. (bloodjournal.org)
  • EVs are a heterogeneous population of vesicles secreted by all cells in a size range of 30-2000 nm. (pubmedcentralcanada.ca)
  • Here, we found that colorectal cancer (CRC) cells treated with oxaliplatin (OXA) and/or 5-fluorouracil (5-Fu) released high levels of high-mobility group box 1 (HMGB1) and heat shock protein 70 (HSP70). (nature.com)
  • Synthesis and fabrication of surface-active microparticles using membrane emulsion technique and rapid conjugation of model protein via strain-promoted azide-alkyne click chemistry in physiological conditions. (bioportfolio.com)
  • The lysosome-associated membrane protein (LAMP) family includes the dendritic cell endocytic receptors DC-LAMP and CD68, as well as LAMP-1 and LAMP-2. (jimmunol.org)
  • TF is a type I membrane protein expressed on non-vascular cells and cells within the vessel wall. (biochemsoctrans.org)
  • In this article, we report an innovative green chemistry approach for the fabrication of protein microparticles based on peptide mediated disulfide interchange reactions. (izon.com)
  • Exosomal transfer of functional Shh protein from Shh-expressing to recipient cells represents a novel and original mechanism of cell-based therapy. (ahajournals.org)
  • This cell or cell membrane-based DDS can be produced in a simple way with minimum membrane protein loss. (thno.org)
  • Taking a systems approach, we develop and summarise the view that "serum ferritin" actually originates from damaged cells (and thus reflects cellular damage), that it contains some iron but has lost or liberated most of its normal content, and that since the protein part of ferritin is assumed to be benign, that it is this (initially) free iron that correlates with and is causative of disease. (rsc.org)
  • The invention relates to a peptide, polypeptide, or protein that binds specifically to cells of the lung endothelium. (patents.com)
  • The developed bioassays successfully detected target VEGF protein present in serum-free buffer and serum-containing complete cell culture medium with high sensitivity and specificity. (thno.org)
  • MIS416 has been suggested to modulate T-cell-mediated autoimmune responses in EAE by simultaneously activating innate Toll-like receptor 9 and nucleotide-binding oligomerization domain-containing protein 2. (wikipedia.org)
  • One of the most promising is dendritic cell based cancer immunotherapy. (dtu.dk)
  • Among patients with metastatic colorectal cancer, approximately half respond to a dendritic cell vaccine in the adjuvant setting following surgical resection of metastases. (aacrjournals.org)
  • The CD142+(tissue factor [TF])-associated MPs and CD146+(endothelial cell)-associated MPs were significantly elevated throughout the study period. (thejns.org)
  • 13 Small molecules released into platelet supernatants were characterized by gas chromatography-mass spectrometry (GC-MS) and direct injection-mass spectrometry (DIMS), which revealed the presence of deoxyribose-1-phosphate (dRP), a known proangiogenic molecule that stimulates endothelial cell migration. (ahajournals.org)
  • At the beginning of atherosclerotic plaque formation, endothelial dysfunction is characterized by increased expression and release of adhesion molecules, including endothelial selectin (E-selectin), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). (scielo.br)
  • This hypothesis is based on the relatively rapid inactivation of the FXa formed on TF-bearing cells when it is released into the plasma. (biochemsoctrans.org)
  • The microparticles also expressed CD66b, CD62L and myeloperoxidase, which are all commonly expressed on the surface of neutrophils, as well as exposition of phosphatidylserine. (biomedcentral.com)
  • More recently, this therapeutic efficacy has been attributed to the bioactive molecules that these cells secrete (secretome), now being referred as medicinal signaling cells. (stanford.edu)