A genomic comparison of in vivo and in vitro brain microvascular endothelial cells. (1/372)The blood-brain barrier (BBB) is composed of uniquely differentiated brain microvascular endothelial cells (BMEC). Often, it is of interest to replicate these attributes in the form of an in vitro model, and such models are widely used in the research community. However, the BMEC used to create in vitro BBB models de-differentiate in culture and lose many specialized characteristics. These changes are poorly understood at a molecular level, and little is known regarding the consequences of removing BMEC from their local in vivo microenvironment. To address these issues, suppression subtractive hybridization (SSH) was used to identify 25 gene transcripts that were differentially expressed between in vivo and in vitro BMEC. Genes affected included those involved in angiogenesis, transport and neurogenesis, and real-time quantitative polymerase chain reaction (qPCR) verified transcripts were primarily and significantly downregulated. Since this quantitative gene panel represented those BMEC characteristics lost upon culture, we used it to assess how culture manipulation, specifically BMEC purification and barrier induction by hydrocortisone, influenced the quality of in vitro models. Puromycin purification of BMEC elicited minimal differences compared with untreated BMEC, as assessed by qPCR. In contrast, qPCR-based gene panel analysis after induction with hydrocortisone indicated a modest shift of 10 of the 23 genes toward a more 'in vivo-like' gene expression profile, which correlated with improved barrier phenotype. Genomic analysis of BMEC de-differentiation in culture has thus yielded a functionally diverse set of genes useful for comparing the in vitro and in vivo BBB. (+info)
Retinoic acid inhibits Th17 polarization and enhances FoxP3 expression through a Stat-3/Stat-5 independent signaling pathway. (2/372)CD4(+) helper T (Th) cells play a crucial role in the delicate balance between host defense and autoimmune disease. Two important populations of helper T cells are the proinflammatory, interleukin-17 (IL-17)-producing (Th17) cells and the anti-inflammatory forkhead box P3-positive (FoxP3(+)) T regulatory (Treg) cells. Here we show that all-trans retinoic acid (ATRA) and other agonists of the retinoic acid receptor alpha (RARalpha) inhibit the formation of Th17 cells and promote FoxP3 expression. Conversely, inhibition of retinoic acid signaling constrains transforming growth factor beta (TGF-beta1) induction of FoxP3. The effect of ATRA is mediated independently of IL-2, signal transducer and activator of transcription 5 (Stat5) and Stat3, representing a novel mechanism for the induction of FoxP3 in CD4 T cells. As previous studies have shown that vitamin A derivatives are protective in animal models of autoimmune disease, the current data suggest a previously unrecognized role for RARalpha in the regulation of CD4(+) T-cell differentiation and provide a mechanism for the anti-inflammatory effects of retinoic acid. (+info)
Aromatic residues in the C-terminal domain 2 are required for Nanog to mediate LIF-independent self-renewal of mouse embryonic stem cells. (3/372)Nanog was identified by its ability to sustain the LIF-independent self-renewal of mouse embryonic stem (ES) cells and has recently been shown to play a role in reprogramming adult fibroblasts into pluripotent stem cells. However, little is known about the structural basis of these remarkable activities of Nanog. We have previously identified an unusually strong transactivator named CD2 at its C terminus. Here we demonstrate that CD2 is required for Nanog to mediate ES cell self-renewal. Furthermore, deletion and point mutation analysis revealed that CD2 relies on at least seven aromatic amino acid residues to generate its potent transactivating activity. A mutant Nanog bearing alanine substitutions for these seven residues fails to confer LIF-independent self-renewal in mouse ES cells. Substitution of CD2 by the viral transactivator VP16 gave rise to Nanog-VP16, which is 10 times more active than wild-type Nanog in ES cells. Surprisingly, the expression of Nanog-VP16 in mouse ES cells induces differentiation and is thus unable to sustain LIF-independent self-renewal for mouse ES cells. Taken together, our results demonstrate that the CD2 domain of Nanog is a unique transactivator that utilizes aromatic residues to confer specific activity absolutely required for ES self-renewal. (+info)
Epithelial-to-mesenchymal transition is a potential pathway leading to podocyte dysfunction and proteinuria. (4/372)(+info)
Functional network reconstruction reveals somatic stemness genetic maps and dedifferentiation-like transcriptome reprogramming induced by GATA2. (5/372)(+info)
A developmental model of sarcomagenesis defines a differentiation-based classification for liposarcomas. (6/372)(+info)
Diffusion-weighted MR imaging: diagnosing atypical or malignant meningiomas and detecting tumor dedifferentiation. (7/372)(+info)
Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. (8/372)(+info)
Dedifferentiation also occurs in plants. Cells in cell culture can lose properties they originally had, such as protein ... fat cells, and types of bone cells Epithelial stem cells (progenitor cells) that give rise to the various types of skin cells ... Such cells, called somatic cells, make up most of the human body, such as skin and muscle cells. Cells differentiate to ... Hematopoietic stem cells (adult stem cells) from the bone marrow that give rise to red blood cells, white blood cells, and ...
Glossary of genetics (0-L)
cellular reprogramming The conversion of a cell from one tissue-specific cell type to another. This involves dedifferentiation ... germ cell Any cell that gives rise to the gametes of an organism that reproduces sexually. Germ cells are the vessels for the ... Cells of the germ line are called germ cells. 2. The lineage of germ cells, spanning many generations, that contains the ... interphase All stages of the cell cycle excluding cell division. A typical cell spends most of its life in interphase, during ...
Glossary of genetics
cellular reprogramming The conversion of a cell from one tissue-specific cell type to another. This involves dedifferentiation ... any cell other than a gamete, germ cell, or undifferentiated stem cell. Somatic cells are theoretically distinct from cells of ... germ cell Any cell that gives rise to the gametes of an organism that reproduces sexually. Germ cells are the vessels for the ... stem-loop stem cell Any biological cell which has not yet differentiated into a specialized cell type and which can divide ...
... differentiated cell. Re-entry into the cell cycle allows the cell to go through mitosis, dividing to make more cells that would ... Cell plasticity is closely related to dedifferentiation, as it implies that a cell with 'plasticity' can dedifferentiate to ... After dedifferentiation, new cells can be generated by re-entering the cell cycle and proliferating, then redifferentiating to ... Dedifferentiation could spark innovation in regenerative medicine because it suggests that one's own cells can change cell ...
Cell fate determination
Friedmann-Morvinski, Dinorah; Verma, Inder M (March 2014). "Dedifferentiation and reprogramming: origins of cancer stem cells ... two of which are by the combination of transcription factors the cells have and by the cell-cell interaction. Cells' fate ... If a cell is in a determined state, the cell's fate cannot be reversed or transformed. In general, this means that a cell ... Eur J Cell Biol 2010, 89:225-230. Lau, S.; Ehrismann, J.; Schlereth, A.; Takada, S.; Mayer, U.; Jürgens, G. (2010). "Cell-cell ...
Pluripotency (biological compounds)
... but they all came from one pluripotent cell. Induced pluripotent stem cell Directed differentiation Dedifferentiation " ... TH2 cells produce cytokines that will trigger certain B cells. B cells can differentiate into memory cells or plasma cells. The ... This stem cell can produce red blood cells, platelets, mast cells, dendritic cells, macrophages, lymphocytes, neutrophils, ... They divide into memory cells, TH1, TH17, and TH2 cells, to name a few. Memory cells are made solely for the purpose of having ...
by inducing a shift in the developmental fate of cells in the liver and converting them into 'pancreatic beta-cell-like' cells ... Despite the massive dedifferentiation - lineage tracing approach indeed demonstrates that transdifferentiation originates in ... In this approach, transcription factors from progenitor cells of the target cell type are transfected into a somatic cell to ... Patel, M.; Yang, S. (2010). "Advances in Reprogramming Somatic Cells to Induced Pluripotent Stem Cells". Stem Cell Reviews and ...
... used for stem cell dedifferentiation. It also has the potential to selectively induce cell death in cancer cells. Reversine is ... Chen, Shuibing; Zhang, Qisheng; Wu, Xu; Schultz, Peter G.; Ding, Sheng (2004). "Dedifferentiation of Lineage-Committed Cells by ... "The synthetic purine reversine selectively induces cell death of cancer cells". Journal of Cellular Biochemistry. 113 (10): ... The Journal of Cell Biology. 190 (1): 73-87. doi:10.1083/jcb.201001036. ISSN 1540-8140. PMC 2911657. PMID 20624901. Henry, ...
... including the stimulation of activated B cell and T cell proliferation, and the differentiation of B cells into plasma cells. ... IL-4 has also been shown to drive mitogenesis, dedifferentiation, and metastasis in rhabdomyosarcoma. IL-4, along with other ... The interleukin 4 (IL4, IL-4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon ... Auxiliary T cells are a key element of HIV-1 infection. Several signs of immune dysregulation such as polyclonal B cell ...
"E.B. Wilson Medal". American Society for Cell Biology. "ASCB Presidents". American Society for Cell Biology. "Hay Professorship ... Hay, Elizabeth Dexter (1962). "Cytological studies of dedifferentiation and differentiation in regenerating amphibian limbs". ... cell shape, and the control of cell growth and differentiation. She asserted that the basis of many scientific ideas originate ... the regeneration of the limb is achieved when differentiated cells begin to dedifferentiate and become stem cells. In 1957, Don ...
Induced stem cells
November 2013). "Dedifferentiation of committed epithelial cells into stem cells in vivo". Nature. 503 (7475): 218-23. Bibcode: ... May 2013). "TAP-deficient human iPS cell-derived myeloid cell lines as unlimited cell source for dendritic cell-like antigen- ... June 2014). "Human somatic cell nuclear transfer using adult cells". Cell Stem Cell. 14 (6): 777-80. doi:10.1016/j.stem.2014.03 ... June 2013). "Human embryonic stem cells derived by somatic cell nuclear transfer". Cell. 153 (6): 1228-38. doi:10.1016/j.cell. ...
Sugimoto K, Gordon SP, Meyerowitz EM (April 2011). "Regeneration in plants and animals: dedifferentiation, transdifferentiation ... Cell potency is a cell's ability to differentiate into other cell types. The more cell types a cell can differentiate into, the ... In cell biology, a unipotent cell is the concept that one stem cell has the capacity to differentiate into only one cell type. ... A close synonym for unipotent cell is precursor cell. Biology portal Induced stem cells Cell totipotency was discovered by ...
Air-liquid interface cell culture
"Dedifferentiation of committed epithelial cells into stem cells in vivo". Nature. 503 (7475): 218-223. Bibcode:2013Natur.503.. ... Air liquid interface cell culture (ALI) is a method of cell culture by which basal stem cells are grown with their basal ... This method of cell culture aims to be used to study fundamental aspects of the respiratory epithelium, such as cell-to-cell ... One recent study has shown that differentiated cells in the respiratory epithelium (secretory cells and ciliated cells ...
Plant stem cell
... as an alternative to plant stem cells. Callus, or dedifferentiated cells, are somatic cells that undergo dedifferentiation to ... Thus plant stem cell culture is the most ideal and productive method of cell culture and phytochemical production as cells are ... Plant stem cells are innately undifferentiated cells located in the meristems of plants. Plant stem cells serve as the origin ... These immortal cells divide infinitely. Plant cells are cultured to acquire plant useful compounds. However cell cultures are ...
In Physcomitrella patens the PcG protein FIE is specifically expressed in stem cells such as the unfertilized egg cell. Soon ... Consequently, loss of PcG causes de-differentiation and promotes embryonic development. Polycomb-group proteins also intervene ... Cell. 128 (4): 735-45. doi:10.1016/j.cell.2007.02.009. PMID 17320510. S2CID 6492075. Pirrotta V, Li HB (2012). "A view of ... Cell. 171 (1): 34-57. doi:10.1016/j.cell.2017.08.002. PMID 28938122. S2CID 43165761. Di Croce L, Helin K (2013). " ...
Oncostatin M receptor
"Oncostatin M is a major mediator of cardiomyocyte dedifferentiation and remodeling". Cell Stem Cell. 9 (5): 420-32. doi:10.1016 ... OSMR is widely expressed across non-haematopoietic, hepatocytes, mesothelial cells, glial cells and epithelial cell types ... Intracellular cell signalling occurs as a consequence of extracellular binding of the ligand OSM to OSMR complexes, formed from ... OSM and OSMRβ are co-expressed and lead to STAT 3 activation malignant human ovarian epithelial cells. The OSMR β promoter gene ...
... "enod40 induces dedifferentiation and division of root cortical cells in legumes". Proc Natl Acad Sci U S A. 94 (16): 8901-8906 ... for elicitation of a cell-specific growth response in the alfalfa root cortex". Mol Cell Biol. 21 (1): 354-366. doi:10.1128/MCB ... Guzzo F, Portaluppi P, Grisi R, Barone S, Zampieri S, Franssen H, Levi M (2005). "Reduction of cell size induced by enod40 in ... Plant Cell. 11 (10): 1953-1966. doi:10.1105/tpc.11.10.1953. PMC 144109. PMID 10521525. Kouchi H, Takane K, So RB, Ladha JK, ...
Minusheet perfusion culture system
Instead dedifferentiation is observed [1-4]. These cell biological alterations arise after isolation of cells and proceed ... Cell Biol Int 34(11):1075-83. 5. Minuth WW and Denk L 2011 Advanced culture experiments with adherent cells. From single cells ... Due to these reasons a Minusheet tissue carrier with adherent cells is used only for the short period of cell seeding in a 24 ... Minusheet perfusion culture system is used for advanced cell culture experiments in combination with adherent cells and to ...
"Inducing cellular dedifferentiation: A potential method for enhancing endogenous regeneration in mammals". Seminars in Cell & ... Like stem cells in SGZ, progenitor cells in SVZ also differentiate into neurons and form an intermediate cell called a ... In the dentate gyrus, putative stem cells, called type 1 cells, proliferate into type 2 and type 3 cells, which are transiently ... Progenitor cells in the dentate gyrus of the hippocampus migrate to the nearby location and differentiate into granule cells. ...
"Intestinal tumorigenesis initiated by dedifferentiation and acquisition of stem-cell-like properties". Cell. 152 (1-2): 25-38. ... In collaboration with Hans Clevers's laboratory, Owen's group showed that Lgr5-positive cells are an efficient cell of ... Cell Stem Cell. 12 (6): 761-73. doi:10.1016/j.stem.2013.04.006. PMC 3690525. PMID 23665120. Sansom, OJ; Stark, LA; Dunlop, MG; ... "Crypt stem cells as the cells-of-origin of intestinal cancer". Nature. 457 (7229): 608-11. Bibcode:2009Natur.457..608B. doi: ...
... which dedifferentiate into stem-like cells and proliferate into neural progenitor cells in response to retinal damage. While ... Wan, J; Ramachandran, R; Goldman, D (2012). "HB-EGF is necessary and sufficient for Müller glia dedifferentiation and retina ... The neural progenitor cell proliferates to form a cluster of neural progenitors, which migrate to the outer nuclear layer of ... The mice had suffered from a complete lack of photoreceptor cells in their retinas, and had been unable to tell light from dark ...
"Contribution of mesenterial muscle dedifferentiation to intestine regeneration in the sea cucumber Holothuria glaberrima". Cell ... Cells that contain the amino acid peptide, Stichopin, have been found within the connective tissues of the Chiridotidae. These ... Researchers have discovered that Chiridotidae reach their asymptotic range size at 10 cells. Sea cucumbers are a mostly ... "Stichopin-containing nerves and secretory cells specific to connective tissues of the sea cucumber". Proceedings of the Royal ...
The role of Tyrp1 in melanoma progression was determined by comparing "knockout" cell lines which have inactive Tyrp1 to cells ... Abe T, Sato M, Tamai M (December 1998). "Dedifferentiation of the retinal pigment epithelium compared to the proliferative ... Tyrp1 mRNA interacts with miR-16 and affects its ability to repress genes involved in melanoma cell production. Mutations in ... Such studies provide insight to possible clinical usage and treatment of melanoma via regulation of Tyrp1 expression in cells. ...
Rho kinase inhibitor
Particularly, ROCK inhibitor is used for cell culture practice, in part to limit cellular death and limited dedifferentiation, ... and non-small cell lung cancer (A549) cells independent of cell death induction. Fasudil, not currently approved by the U.S. ... On a cellular level, ROCK has multiple functions, including regulation of smooth muscle cell contraction, cell migration, and ... "Minimal Sustainability of Dedifferentiation by ROCK Inhibitor on Rat Nucleus Pulposus Cells In Vitro". Spine Surgery and ...
Aspirin exacerbated respiratory disease
However, Differential gene expression analysis of AERD nasal polyp epithelial cells versus AERD non polyp nasal mucosa revealed ... Furthermore, several genes are downregulated, hinting at the dedifferentiation phenomenon in AERD polyps. The majority of those ... immunoglobulin E or T cells. Rather, AERD is a type of NSAID-induced hypersensitivity syndrome. EAACI/WHO classifies the ... aspirin treatment of eosinophils from aspirin intolerant subjects causes the cells to mount a further increase in eoxin ...
Forkhead box protein O1
Sun T, Han X (2019). "Death versus dedifferentiation: The molecular bases of beta cell mass reduction in type 2 diabetes". ... Fatty acids in the beta cells activate FOXO1, resulting in apoptosis of the beta cells. FOXO1 activation plays a role in cell ... It suppresses survival of tumor cells by inducing apoptosis in prostate cancer cells and glioma cells by upregulating the ... In pancreatic beta cells FOXO1 mediates glucagon-like peptide-1 effects on pancreatic beta-cell mass. When the level of blood ...
... dedifferentiation or self-renewal of ES cells". Nature Genetics. 24 (4): 372-376. doi:10.1038/74199. PMID 10742100. S2CID ... Also, Rex1−/Oct3/4+ cells differentiate into cells of primitive ectoderm, the somatic cell lineage. Studies have shown that ... Thus, the male germ cells undergoing meiosis are the specific cells in the testicles that express Rex1. It has not been ... During embryogenesis, the inner cell mass (ICM) is separated from the trophoblast. The stem cells derived from the ICM and ...
... dedifferentiation or self-renewal of ES cells". Nature Genetics. 24 (4): 372-6. doi:10.1038/74199. PMID 10742100. S2CID ... embryonic stem cell lines (which are cell lines derived from the inner cell mass), and induced pluripotent stem cells. While ... "Oct4 expression is not required for mouse somatic stem cell self-renewal". Cell Stem Cell. 1 (4): 403-15. doi:10.1016/j.stem. ... "Oct-4 expression in adult human differentiated cells challenges its role as a pure stem cell marker". Stem Cells. 25 (7): 1675- ...
2,4-D is used in plant cell cultures as a dedifferentiation (callus induction) hormone. It is classified as an auxin plant ... Endreb, Rudolf (1994). Plant Cell Biotechnology. Germany: Springer. pp. 17, 18. ISBN 978-0-387-56947-5. NIOSH. Updated June ... 4-D has been used in laboratories for plant research as a supplement in plant cell culture media such as MS medium since at ...
Stem cell marker
"Up-regulation of neural stem cell markers suggests the occurrence of dedifferentiation in regenerating spinal cord". ... Stem cell markers are genes and their protein products used by scientists to isolate and identify stem cells. Stem cells can ... 2005). "Somatic stem cell marker prominin-1/CD133 is expressed in embryonic stem cell-derived progenitors". Stem Cells. 23 (6 ... Hirao A, Arai F, Suda T (December 2004). "Regulation of cell cycle in hematopoietic stem cells by the niche". Cell Cycle. 3 (12 ...
"Melanomas resist T-cell therapy through inflammation-induced reversible dedifferentiation.", Nature Oct 18;490(7420):412-6., ... "Immune cell-poor melanomas benefit from PD-1 blockade after targeted type I IFN activation.", Cancer Discov. Jun;4(6):674-87, ...
Dedifferentiation Pluripotency Cohen DE, Melton D (2011). "Turning straw into gold: directing cell fate for regenerative ... co-culture with stromal cells or feeder cells, and on specific culture substrates: support cells and matrices provide ... introduced in the cells. The starting material can be either pluripotent stem cells (PSCs), or either differentiated cell type ... Induced stem cells, Stem cells, Biotechnology, Cell biology, Biological engineering, Biomedical engineering, Tissue engineering ...
Callus (cell biology)
... however callus and somatic embryos are derived from mesophyll cells that undergo dedifferentiation. Plant hormones are used to ... Plant callus (plural calluses or calli) is a growing mass of unorganized plant parenchyma cells. In living plants, callus cells ... Regeneration of a whole plant that has some genetically transformed cells and some untransformed cells yields a chimera. In ... In Jatropha curcas callus cells, small organized callus cells became disorganized and varied in size after browning occurred. ...
It has been shown in zebrafish and mice that Müller glia undergo dedifferentiation into multipotent progenitor cells. The ... The Müller glia cell is the only retinal glial cell that shares a common cell lineage with retinal neurons. A subset of Müller ... as all glial cells do. They are the most common type of glial cell found in the retina. While their cell bodies are located in ... Damage to retinal cells induces Müller cells to produce gliosis. The result of the response varies depending on the damage and ...
... rather than dedifferentiation of specialized cells, account for undifferentiated tumors. Anaplastic cells display marked ... It implies dedifferentiation, or loss of structural and functional differentiation of normal cells. It is now known, however, ... Also, anaplastic cells usually fail to develop recognizable patterns of orientation to one another (i.e., they lose normal ... The nuclear-cytoplasmic ratio may approach 1:1 instead of the normal 1:4 or 1:6. Giant cells that are considerably larger than ...
Cobaleda, C; Jochum, W; Busslinger, M (27 September 2007). "Conversion of mature B cells into T cells by dedifferentiation to ... The lymphoid lineage is composed of T-cells, B-cells and natural killer cells. This is lymphopoiesis. Cells of the myeloid ... All blood cells are divided into three lineages. Red blood cells, also called erythrocytes, are the oxygen-carrying cells. ... Each successive change moves the cell closer to the final cell type and further limits its potential to become a different cell ...
"In CK2 inactivated cells the cyclin dependent kinase inhibitor Sic1 is involved in cell-cycle arrest before the onset of S ... Low p27Kip1 expression can lead to tumor de-differentiation, increased penetration through the gastric wall, lymph node ... Cell. 79 (2): 233-44. doi:10.1016/0092-8674(94)90193-7. PMID 7954792. S2CID 34939988. Morgan DO (1997). The Cell Cycle: ... In the G1 phase of the cell cycle, Sic1 binds tightly to the Cdc28-Clb complex and inhibits it. Low Cdc28-Clb activity leads to ...
However, benign desmoplasias do not exhibit dedifferentiation of tumor cells. A desmoplastic response is characterized by ... or parenchymal cells of epithelia, are highly organized, polar cells. These cells are separated from stromal cells by a ... Normal tissues consist of parenchymal cells and stromal cells. The parenchymal cells are the functional units of an organ, ... doi:10.1016/j.cell.2011.02.013. PMID 21376230. Alberts, B; Johnson, A; Lewis, J (2008). Molecular Biology of the Cell (5th ed ...
Schwann cells respond to loss of axons by extrusion of their myelin sheaths, downregulation of myelin genes, dedifferentiation ... Axons have been observed to regenerate in close association to these cells. Schwann cells upregulate the production of cell ... During their proliferation phase, Schwann cells begin to form a line of cells called Bands of Bungner within the basal laminar ... 2005) observed that non-myelinated or myelinated Schwann cells in contact with an injured axon enter cell cycle thus leading to ...
... type 1 lung alveolar cells, skeletal muscle cells, placenta, etc. Podoplanin expression in breast cancer cells induces cell ... The mesenchymal mechanism of invasion is believed to be the consequence of EMT, when active dedifferentiation of a malignant ... Single cell invasion or individual cell migration Such a type of invasive growth as single cell invasion is distinguished based ... Along with single cell migration, collective cell migration can occur when groups of firmly interconnected tumor cells are ...
Sangeeta N. Bhatia
Bhatia also used co-cultures of more than one cell type to prevent dedifferentiation of the liver cells, building on the work ... Hui, E. E.; Bhatia, S. N. (March 27, 2007). "Micromechanical control of cell-cell interactions". Proceedings of the National ... They may be able to home in on a tumor, signal changes in cells or tissues, enhance imaging, or release a therapeutic component ... She is interested in using arrays of living cells as high-throughput platforms to study fundamental aspects of Bio-MEMS in stem ...
However, two common theories that have often been expressed are cell dedifferentiation and the recruitment of stem cells to the ... In urodele amphibians, studies suggest that dedifferentiation of cells leads to the formation of a blastema that is able to ... Blastema cells can differentiate into any cell type with the exception of neurons. This means axons which are cut can be ... The cells in the blastema are also referred to as clonogenic neoblasts (cNeoblasts) that are able to move to the site of the ...
Agent-based model in biology
... with each agent representing a mammary stem cell, a progenitor cell, or a differentiated cell in the breast. Simulations were ... dedifferentiation via epithelial-mesenchymal transition (EMT), and self-renewal (symmetric division) as mechanisms by which ... Surprisingly, radiation-induced cell inactivation by death did not contribute to increased stem cell frequency independently of ... Some examples of specific applications of cell-based modeling are: An agent-based model can be used model the colonisation of ...
Cancer stem cell
De-differentiation of mutated cells may create stem cell-like characteristics, suggesting that any cell might become a cancer ... "Critical appraisal of the side population assay in stem cell and cancer stem cell research". Cell Stem Cell. 8 (2): 136-47. doi ... They examined cancer stem cell plasticity in which cancer stem cells can transition between non-cancer stem cells (Non-CSC) and ... Origin hypotheses include mutants in developing stem or progenitor cells, mutants in adult stem cells or adult progenitor cells ...
... dedifferentiation and rapid cell proliferation, have been pointed out. Tumor growth has been studied using mathematics and ... for B cell lesions) or T cell receptor gene (for T cell lesions). The demonstration of clonality is now considered to be ... These cells are presumed to be monoclonal - that is, they are derived from the same cell, and all carry the same genetic or ... A mutant or epigenetically altered stem cell may replace the other nearby stem cells by natural selection. Thus, a patch of ...
Supplementing GDF11 increased tubular cell dedifferentiation and proliferation as well as improved the prognosis of old mice ... GDF11 fed mice saw 45.7% reduction in senescent liver cells and a 21.7% reduction in senescent kidney cells. GDF11 induces ... GDF11 induces tumor suppressive properties in human hepatocellular carcinoma-derived cells, Huh7 and Hep3B cell lines, ... and controls the competence of progenitor cells to regulate numbers of retinal ganglionic cells developing in the retina. Other ...
323-LB: Activation of the GLP-1 Receptor Signaling Prevents Beta-Cell Dedifferentiation in a Mouse Model of Wolfram Syndrome...
Loss of functional β cells results in a gradual progression of insulin insufficiency in Wolfram syndrome caused by recessive ... mice inhibited β cell dedifferentiation, resulting in prevention of diabetes progression with preservation of β cell mass. Our ... β cells become dedifferentiated and revert to endocrine progenitor-like cells expressing Ngn3, and a subset of β cells takes α ... 323-LB: Activation of the GLP-1 Receptor Signaling Prevents Beta-Cell Dedifferentiation in a Mouse Model of Wolfram Syndrome ...
B cell acute lymphoblastic leukemia relapse following CD19 CAR immune-pressure via de-differentiation and lineage switch | NIH...
B cell acute lymphoblastic leukemia relapse following CD19 CAR immune-pressure via de-differentiation and lineage switch. ... Our data demonstrates plasticity of leukemic cells under antigen-specific immune pressure, with de-differentiation and lineage ... Later relapses had loss of all the B cell surface markers, along with down regulation of B cell transcripts (such as EBF1 and ... CD19 is a surface marker restricted to B cells and highly expressed on B cell ALL. CD19 has been targeted through genetically ...
Schwann cell-specific RhoA knockout accelerates peripheral nerve regeneration via promoting Schwann cell dedifferentiation. |...
Schwann cell-specific RhoA knockout accelerates peripheral nerve regeneration via promoting Schwann cell dedifferentiation.. ... Mechanistic studies in both in vivo and in vitro models revealed that RhoA cKO could facilitate Schwann cell dedifferentiation ... Schwann cell dedifferentiation subsequently promotes Wallerian degeneration by enhancing phagocytosis and myelinophagy, as well ... These findings shed light on the role of RhoA in Schwann cells during nerve injury and repair, indicating that cell type- ...
Aging melanocyte stem cells and gray hair | National Institutes of Health (NIH)
The stem cells responsible for hair color switch between stem cell and maturing states, but with age can get stuck and become ... Introduction to Stem Cells. References: Dedifferentiation maintains melanocyte stem cells in a dynamic niche. Sun Q, Lee W, Hu ... During hair growth, the progenitor cells differentiate (develop) into mature cells. Meanwhile, the original stem cells remain ... Aging melanocyte stem cells and gray hair. At a Glance. *Researchers found that the stem cells responsible for hair color can ...
RFA-GM-07-002: Program Projects for Basic Research on Human Embryonic Stem Cells (P01)
Program Projects for Basic Research on Human Embryonic Stem Cells (P01) RFA-GM-07-002. NIGMS ... determinants of differentiation, dedifferentiation, and nuclear reprogramming *chromosome, chromatin, and telomere structure ... embryonic germ cells, adult stem cells, or animal embryonic stem cells) may be included. Applicants should visit the NIGMS ... embryonic germ cells, adult stem cells) and from more differentiated cells ...
Cell Dedifferentiation; Cell Line; Cell Movement; Cell Proliferation; Cell Transformation, Neoplastic/chemically induced*; Cell ... Neoplastic Stem Cells/cytology*; Neoplastic Stem Cells/metabolism; Neoplastic Stem Cells/pathology; Proto-Oncogene Proteins c- ... Transformation, Neoplastic/metabolism; Epithelial Cells/cytology; Epithelial Cells/drug effects; Epithelial Cells/metabolism; ... and that some of the transformed cells show characteristics of cancer stem-like cells (CSCs). Meanwhile, we demonstrate that ...
Workshop: The Cancer-Alzheimer's disease nexus: Exploring relationships, mechanisms, and therapeutic implications | National...
Neuronal de-differentiation leads to cell death competency of mature brain neurons partially driven by PKM2. ... Human neural cell and mouse models demonstrated that cytoplasmic dsRNA is sufficient to evoke propagated neural cell death in a ... Because cells do not function alone, the effect of PPI networks reverberates and extends beyond individual cells into tissues, ... Pathways that operate in the inverse direction include cell death (i.e., cancer resists, AD increases), cell proliferation (i.e ...
Ghent University Academic Bibliography
JCI - Kidney injury molecule-1 is a phosphatidylserine receptor that confers a phagocytic phenotype on epithelial cells
Dedifferentiation and proliferation of surviving epithelial cells in acute renal failure. J. Am. Soc Nephrol. 14:S55-S61. View ... Although KIM1-PK1 cells did not bind yeast cell wall, zymosan (Figure 5C), KIM1-PK1 cells bound and internalized both Gram ... The epithelial cells were used in assays between day 4 and day 5. KIM1-PK1 cells were generated by transfecting LLC-PK1 cells ... Epithelial cells as phagocytes: apoptotic epithelial cells are engulfed by mammary alveolar epithelial cells and repress ...
3. ddb2 represses ovarian cancer cell dedifferentiation by suppressing ALDH1A1.. Cui T; Srivastava AK; Han C; Wu D; Wani N; Liu ... 7. ddb2 suppresses tumorigenicity by limiting the cancer stem cell population in ovarian cancer.. Han C; Zhao R; Liu X; ... 5. ddb2 modulates TGF-β signal transduction in human ovarian cancer cells by downregulating NEDD4L.. Zhao R; Cui T; Han C; ... 8. DNA damage-binding complex recruits HDAC1 to repress Bcl-2 transcription in human ovarian cancer cells.. Zhao R; Han C; ...
Khoury RE[au] - Search Results - PubMed
NIDCD Auditory/Stem Cell Workshop 2001 | NIDCD
Determination of the origin of regenerated hair cells from reserve stem cells or through dedifferentiation of other cells. ... Usage of adult stem cells from other tissue systems (mesenchymal stem cells, hematopoietic stem cells, neural stem cells, etc ... Characterization of cell-cell interactions involved in stabilizing hair cell-support cell interactions. ... Existence of auditory and vestibular progenitor cells. *Identification of stem or progenitor cells that give rise to hair cells ...
CIMB | Free Full-Text | Regenerative Potential of Blood-Derived Products in 3D Osteoarthritic Chondrocyte Culture System
... was evaluated using a 3D osteoarthritic chondrocyte pellet model by assessing the metabolic cell activity, cartilage-related ... Cytokine networking of chondrocyte dedifferentiation in vitro and its implications for cell-based cartilage therapy. Am. J. ... The Effects of Hyperacute Serum on Adipogenesis and Cell Proliferation of Mesenchymal Stromal Cells. Tissue Eng. Part A 2018, ... It seems that PRP can influence the cell-hostile microenvironment of inflammation or cell disintegration and to slow or even ...
Machine Learning Identifies Stemness Features Associated with Oncogenic Dedifferentiation.
Cancer progression involves the gradual loss of a differentiated phenotype and acquisition of progenitor and stem-cell-like ... Here, we provide novel stemness indices for assessing the degree of oncogenic dedifferentiation. We used an innovative one- ... Application of our stemness indices to single-cell data revealed patterns of intra-tumor molecular heterogeneity. Finally, the ... algorithm to extract transcriptomic and epigenetic feature sets derived from non-transformed pluripotent stem cells and their ...
Enhancer decommissioning by LSD1 during embryonic stem cell differentiation | Nature
show that in embryonic stem cells, the histone demethylase LSD1 is essential for enhancer deactivation, acting with other ... which is essential for the complete shutdown of the ESC gene expression program and the transition to new cell states. ... is essential in decommissioning enhancers during the differentiation of mouse embryonic stem cells (ESCs). LSD1 occupies ... In embryonic stem cells, the histone demethylase LSD1 occupies the enhancers of active genes and, together with the NuRD ...
Dedifferentiation, Cell See Also. Cell Transdifferentiation. Public MeSH Note. 2008. History Note. 2008. Date Established. 2008 ... Cell Dedifferentiation Preferred Term Term UI T686682. Date11/27/2006. LexicalTag NON. ThesaurusID NLM (2008). ... Dedifferentiation, Cell Term UI T686683. Date11/27/2006. LexicalTag NON. ThesaurusID NLM (2008). ... Cell Dedifferentiation Preferred Concept UI. M0504224. Scope Note. A reverse developmental process in which terminally ...
Gastroduodenal mucosal damage with salsalate versus aspirin: results of experimental models and endoscopic studies in humans -...
Genetic modifications : unintentional effects (...) - Inf'OGM
Cell biology : delivering tough cargo into cells », Marx, V. (2016), Nat Meth, 13(1), 37-40 ; « Advancing crop transformation ... Regeneration in plants and animals : dedifferentiation, transdifferentiation, or just differentiation ? », Sugimoto, K. et al. ... Cell biology : delivering tough cargo into cells », Marx, V. (2016), Nature Methods 13, 37-40 ; « Advancing crop transformation ... 1 - Preparing the cells : breaking / removal of the external wall. « Meiotic transmission of epigenetic changes in the cell- ...
Name It / Claim It: Epigenetics Now Just Another Evolutionary Mechanism | Uncommon Descent
... nurse cells pump Bicoid and Nanos RNAs into the egg. (Nurse cells provide the cell that will become the egg-known as the oocyte ... Does that mean that we understand the process of de-differentiation? No. We just add the TFs, and wait. Time goes by (a few of ... or in different specific tissue cells, both stem cells and differentiated cells. The epigenetic state of the zygote, in turn, ... or in different specific tissue cells, both stem cells and differentiated cells. The epigenetic state of the zygote, in turn, ...
Pediatric Posttransplant Lymphoproliferative Disease: Overview, Incidence, Risk Factors
... typically B cells, rarely non-B lineage) in a context of posttransplant immunosuppression. ... It is characterized by uncontrolled proliferation of lymphoid lineage cells ( ... Depending on the degree of proliferation and dedifferentiation, lesions may be characterized as hyperplastic, lymphomatous, or ... Penn estimated that 87% of all PTLDs were of B-cell origin, 13% were of T-cell origin, and 0.5% were of null cell origin.  ...
Neural Crest Development & Disease Unit: People | National Institute of Dental and Craniofacial Research
My research interest is focused on the mechanisms by which cell de-differentiation and cell identity are regulated. ... The aim of my PhD project in the Kerosuo Lab is to understand cell-to-cell heterogeneity within the NC stem cell niche. How do ... Karlas quote: "Thrilled to unravel the secrets of cell identity and pluripotency of the neural crest cells every day in the ... Surangis quote: "The more I learn about neural crest cells, the more questions I have about these remarkable cells and their ...
Frontiers | Differentiated mouse kidney tubuloids as a novel in vitro model to study collecting duct physiology
Kidney tubuloids are cell models that are derived from human or mouse renal epithelial cells and show high similarities with ... Kidney tubuloids are cell models that are derived from human or mouse renal epithelial cells and show high similarities with ... Subsequently, the single cell suspension is seeded into a normal cell culture plate and grown to a confluent 2D cell monolayer ... and inhibited the TGF-β receptors ALK4/5/7 that promote dedifferentiation and epithelial-to-mesenchymal transition (Tojo et al ...
Journal of Biomedical Nanotechnology
of Prostate Cancer Cells by Regulating MYC/Myc-Associated Factor X (MAX) Hongwei Li, Lei Xu, Ming Li, and Zhan Gao. J. Biomed. ... Pathway Regulates Chondrocytes Dedifferentiation Haibo Su, Ke Wang, Gaowei Qu, Peng Li, and Hongyan Li. J. Biomed. Nanotechnol ... of Schwan Cells, Calcium Alginate Hydrogel, and Bellis perennis Extract-Loaded Gelatin Nanofibers Huanan Li, Bao Han, Zhihao ... Stress in Diaphragm Cells by Targeting Nuclear Factor Erythroid 2-Related Factor 2 Chaohui Ji, Huibin Pan, Jie Zhang, Yiming ...
Cell cycle, growth and differentiation in Trypanosoma brucei and Leishmania species - Enlighten Theses
... but are also capable of de-differentiation, albeit at a very low rate. Examination of meta cyclic production at the cellular ... Three populations of BrdU labelled cells were identified; cells which were BrdU labelled in the nucleus only, labelled in the ... An attempt to examine directly the proposed link between antigenic switching and the cell cycle was made using cell cycle ... Three unitary organelles need to be replicated and segregated to the daughter cells at each cell division; the nucleus, ...
Professor David Parkinson - University of Plymouth
2004) The Ras/Raf/ERK signalling pathway drives Schwann cell dedifferentiation EMBO J 23, (15) 3061-3071 Author Site , DOI ... 2001) Krox-20 protects Schwann cells from TGF beta mediated cell death MOLECULAR BIOLOGY OF THE CELL 12, 28A-28A Author Site ... 2001) Krox-20 protects Schwann cells from TGF beta mediated cell death Molecular Biology of the Cell 12, 28A-28A ... Parkinson DB & Svaren J (2020) Specification of macroglia by transcription factors: Schwann cells Patterning and Cell Type ...
Axon-like protrusions promote small cell lung cancer migration and metastasis | eLife
... the transition from a neuroendocrine state to a more neuronal state endows these cancer cells with increased migration and ... dedifferentiation to a non-neuroendocrine state can generate an intra-tumoral niche that protects neuroendocrine SCLC cells ( ... 6PF cells were derived from metastatic cells in the plural fluid in an Rb/p53/p130 TKO; Rosa26mTmG mouse. All cell lines were ... Cell of origin of small cell lung cancer: inactivation of Trp53 and rb1 in distinct cell types of adult mouse lung. Cancer Cell ...
An HIV-Tat inducible mouse model system of childhood HIV-associated nephropathy | Disease Models & Mechanisms | The Company of...
... dedifferentiation changes characteristic of HIVAN might be a secondary event associated with the regeneration of these cells. ... In contrast, very few apoptotic cells were detected in glomerular epithelial cells. Therefore, the process of apoptosis does ... Tat is released into the circulation by infected cells and can be taken up by uninfected cells (Barillari et al., 1999b; Ensoli ... a large number of proliferating cells were noted in glomerular and tubular epithelial cells of HIV-Tg26 mice injected with rAd- ...
ProliferationSpermatogonial dedifferentiationDifferentiationMelanocyte stem cellsSomatic cellsGermlineLineageTumorEmbryonic stemBecoming dedifferentiatedVivoStemnessBiologyPhenotypeRevertMechanismsInducesProgenitor cellsMesenchymal stemPlasticityEpithelial CellsRegenerateNeural crest progenitorGenePreventionDedifferentiateFunctionalSenescencePathwayScholarsEpigeneticReceptorPluripotentTranscriptionProgressionImmuneCharacterizationCSCsSchwannReprogramInhibitionVitroInvasionCharacteristicsMcSCMiceCancerTissueNeoplasticProgenitorsSurface markersTumour
- It particular, Wnt signaling positively regulates cardiomyocyte dedifferentiation and proliferation. (uni-ulm.de)
- Knockout of PHF20 inhibits GBM cell proliferation, as well as its invasiveness and stem cell-like traits. (frontiersin.org)
- As such, elucidating the mechanism of GBM stem cell (GSC) proliferation and maintenance is critical to improve our understanding of the development of GBM. (frontiersin.org)
- It is characterized by uncontrolled proliferation of lymphoid lineage cells (typically B cells, rarely non-B lineage) in a context of posttransplant immunosuppression. (medscape.com)
- The invention relates to methods and compositions for identifying subjects having, or predisposed to having, a neoplastic or cell proliferation or neoplastic disorder. (justia.com)
- Currently, no single biochemical marker, or plurality of biochemical markers, reliably identifies a subject at risk for developing a disease associated with LOI and/or uncontrolled cell proliferation or neoplastic disorders (e.g., benign and cancer). (justia.com)
- Thus, there exists a need for diagnostic methods and compositions that can utilize cell differentiation information to identify those individuals at risk for developing a cell proliferation or neoplastic disorder. (justia.com)
- The present invention is based on the discovery that alterations in ratios of differentiated and undifferentiated cell populations can be used as early indicators for the risk of developing cell proliferation or neoplastic disorders. (justia.com)
- I have studied the transcription factor Krox-20 and how this protein co-ordinates the proliferation, survival and myelination of Schwann cells. (plymouth.ac.uk)
- Neuroblastoma and other cancers occur when a buildup of genetic mutations in critical genes-those that control cell growth and division (proliferation) or maturation (differentiation)-allow cells to grow and divide uncontrollably to form a tumor. (medlineplus.gov)
- Although the specific function of this protein is unknown, it appears to play an important role in cell proliferation. (medlineplus.gov)
- Constitutively active ALK receptor tyrosine kinase may induce abnormal proliferation of immature nerve cells and lead to neuroblastoma. (medlineplus.gov)
- By conditionally manipulating Jak-STAT signaling, we find that spermatogonia that have initiated differentiation and are undergoing limited mitotic (transit-amplifying) divisions can repopulate the niche and revert to stem cell identity. (northwestern.edu)
- Our data demonstrates plasticity of leukemic cells under antigen-specific immune pressure, with de-differentiation and lineage switch. (nih.gov)
- The team discovered that McSC differentiation was triggered in the hair germ by molecular signals from nearby cells. (nih.gov)
- 2. The differentiation stage of p53-Rb-deficient bone marrow mesenchymal stem cells imposes the phenotype of in vivo sarcoma development. (nih.gov)
- 12. MicroRNA expression profiling of human bone marrow mesenchymal stem cells during osteogenic differentiation reveals Osterix regulation by miR-31. (nih.gov)
- differentiation/dedifferentiation. (nih.gov)
- ref. 5 ), which demethylates histone H3 on Lys 4 or Lys 9 (H3K4/K9), is essential in decommissioning enhancers during the differentiation of mouse embryonic stem cells (ESCs). (nature.com)
- We propose that the LSD1-NuRD complex decommissions enhancers of the pluripotency program during differentiation, which is essential for the complete shutdown of the ESC gene expression program and the transition to new cell states. (nature.com)
- My research interest is focused on the mechanisms by which cell de-differentiation and cell identity are regulated. (nih.gov)
- Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. (rndsystems.com)
- In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2). (rndsystems.com)
- The PHOX2B gene is important for the formation and differentiation of nerve cells. (medlineplus.gov)
- Mutations in this gene are believed to interfere with the PHOX2B protein's role in promoting nerve cell differentiation. (medlineplus.gov)
- This disruption of differentiation results in an excess of immature nerve cells and leads to neuroblastoma. (medlineplus.gov)
Melanocyte stem cells2
- The second mechanism, called genomic imprinting, is an epigenetic modification of a specific parental chromosome in the gamete or zygote that leads to monoallelic or differential expression of the two alleles of a gene in somatic cells of the offspring. (justia.com)
- germline stem cells lacking Jak-STAT signaling differentiate into spermatogonia without self-renewal. (northwestern.edu)
- The testis of the fruit fly Drosophila melanogaster offers a simple but powerful system to study dedifferentiation, the process by which differentiating spermatogonia can revert their fate to become fully functional germline stem cells (GSCs). (bvsalud.org)
- To facilitate the study of the cellular and molecular mechanisms underlying the process of germline dedifferentiation in the Drosophila testis , here we describe techniques for inducing high rates of dedifferentiation and for unambiguously labeling dedifferentiated GSCs. (bvsalud.org)
- These late relapses showed expression of cell surface markers compatible with a myeloid lineage (Gr1, Mac1), T cell or undifferentiated lineage (KIT, CD9), confirmed by RNA-sequencing. (nih.gov)
- Kidney injury molecule-1 (KIM-1 or TIM-1) is an immunoglobulin superfamily cell-surface protein not expressed by cells of the myeloid lineage but highly upregulated on the surface of injured kidney epithelial cells. (jci.org)
- My long-term research interest lies in understanding cellular specification and commitment during development of a cell lineage. (nih.gov)
- A reverse developmental process in which terminally differentiated cells with specialized functions revert back to a less differentiated stage within their own CELL LINEAGE . (nih.gov)
- Analyses of the tumor microenvironment revealed unanticipated correlation of cancer stemness with immune checkpoint expression and infiltrating immune cells. (nih.gov)
- Application of our stemness indices to single-cell data revealed patterns of intra-tumor molecular heterogeneity. (nih.gov)
- Glioblastoma (GBM) stem cells are resistant to cancer therapy, and therefore responsible for tumor progression and recurrence after conventional therapy. (frontiersin.org)
- Based on the similarities between somatic cellular reprogramming and cancer stem cells reprogramming, PHF20 plays an important role in carcinogenesis by dramatically enhancing the self-renewal and tumor-initiating capabilities of lung cancer cells, as well as controlling the stem cell-like phenotype of neuroblastoma cells ( 15 , 16 ). (frontiersin.org)
- Dr. Pan has been focusing on studying the mechanisms by which cancer evades immune responses in the tumor microenvironment (TME), more specifically the contribution of myeloid derived suppressor cells (MDSC) to tumor progression. (houstonmethodist.org)
- More recently, Dr. Pan identified a potential role of myeloid inhibitory receptors in the maintenance of tumor-initiating cells (TIC) and radio/chemo-resistance of colon cancer. (houstonmethodist.org)
- Current strategies to diminish GVHD include T-cell depletion and immunosuppressive drugs, which are associated with an increased risk of tumor relapse, opportunistic infection, and/or toxicity. (houstonmethodist.org)
- Smaller tumor cells are found in the cytoplasm of larger tumor cells with crescent-shaped nucleus. (who.int)
- Neuroblastoma occurs when immature nerve cells called neuroblasts become abnormal and multiply uncontrollably to form a tumor. (medlineplus.gov)
- Researchers believe the deleted regions in these chromosomes could contain a gene that keeps cells from growing and dividing too quickly or in an uncontrolled way, called a tumor suppressor gene. (medlineplus.gov)
- The P01 should include a core to grow, maintain, and further characterize approved hESC lines listed on the NIH Human Embryonic Stem Cell Registry, http://stemcells.nih.gov/research/registry/ . (nih.gov)
- Young, R. A. Control of the embryonic stem cell state. (nature.com)
- SSEA‑1 was detected in immersion fixed D3 mouse embryonic stem cell line using 10 µg/mL Human/Mouse SSEA‑1 Monoclonal Antibody (Catalog # MAB2155) for 3 hours at room temperature. (rndsystems.com)
- Our finding implies that activation of the GLP-1 receptor signaling may prevent β cells from becoming dedifferentiated by modulating Txnip and may slow diabetes progression in Wolfram syndrome. (diabetesjournals.org)
- We demonstrated that MDSCs induced antigen specific T regulatory cells and T-cell anergy in vivo. (houstonmethodist.org)
- Machine Learning Identifies Stemness Features Associated with Oncogenic Dedifferentiation. (nih.gov)
- Here, we provide novel stemness indices for assessing the degree of oncogenic dedifferentiation. (nih.gov)
- However, the molecular mechanisms driving the maintenance of stemness and dedifferentiation are poorly understood. (frontiersin.org)
- 1 The Ronald O. Perelman Department of Dermatology and Department of Cell Biology, New York University Grossman School of Medicine, New York, NY, USA. (nih.gov)
- 5 Black Family Stem Cell Institute, Department of Cell, Developmental and Regenerative Biology and Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. (nih.gov)
- The goal of the proposed Program Project (P01) grants is to support research on the basic biology of human embryonic stem cells (hESC) and to promote the use of hESC as a model system to address significant questions in the basic biomedical sciences. (nih.gov)
- The purpose of the workshop was to discuss and identify potential areas of the auditory and vestibular systems that might be good candidates for applications being used in other reparative medicine systems, one being the potential use of stem cell biology. (nih.gov)
- The workshop panel was composed of a diverse group of experts from auditory and vestibular systems as well as tissue engineers using aspects of stem cell biology in other reparative systems. (nih.gov)
- I received my PhD in stem cell biology from the Faculty of Medicine at The University of Helsinki, Finland. (nih.gov)
- Students will be trained in both the theoretical and practical aspects of stem cell biology. (ca.gov)
- Courses on stem cell biology, isolation and maintenance will ensure that the interns have the skill set to be successful and receive maximum benefit from their subsequent internship. (ca.gov)
- His laboratory has been studying the biology of melanoma and has made significant contributions to understanding stem cells as they relate to cancer. (medscape.com)
- Cancer progression involves the gradual loss of a differentiated phenotype and acquisition of progenitor and stem-cell-like features. (nih.gov)
- In this study, we identified plant homeodomain finger-containing protein 20 (PHF20) as a crucial epigenetic regulator for sustaining the stem cell-like phenotype of GBM. (frontiersin.org)
- We have so far evidenced that, in the Wfs1 -/- mice, β cells become dedifferentiated and revert to endocrine progenitor-like cells expressing Ngn3, and a subset of β cells takes α cell fate. (diabetesjournals.org)
- Although the ability of engrafted stem cells to regenerate tissue has received much attention, the molecular mechanisms controlling regeneration are poorly understood. (northwestern.edu)
- Those guys actually use a 'stem cell mediated method' of regenerating," says Catherine McCusker , a professor of molecular mechanisms of regeneration at University of Massachusetts, Boston. (discovermagazine.com)
- The mission of CBDS will be to better define and detect the mechanisms of beta cell stress and destruction central to the development of T1D in humans, with the long-term goal of protecting the residual beta cell mass in T1D patients as early as possible in the disease process. (nih.gov)
- During my PhD and as a postdoctoral fellow at National Eye Institute, I developed a strong interest in regenerative medicine studying the signals and mechanisms that control cell identity. (nih.gov)
- Currently, at Dr. Kerosuo's lab I am interested in the mechanisms that maintain the pluripotent state and the cell identity of the neural crest cells, and how these mechanisms could contribute to regenerative therapies and disease. (nih.gov)
- Precise understanding of the mechanisms that induce and maintain the pluripotency of the stem cell niche in neural crest cells is fundamental to model neural crest defects to develop regenerative therapeutic strategies. (nih.gov)
- The focus of my research is to harness the suppressive function of myeloid-derived suppressor cells (MDSCs) for the treatment/prevention of graft-versus-host diseases (GVHDs) and to study the mechanisms underlying the differential regulation of GVHD and graft-versus-leukemia/lymphoma (GVL) activities by myeloid-derived suppressor cells (MDSCs). (houstonmethodist.org)
- Myeloid-derived suppressor cells (MDSCs) suppress T-cell responses through multiple mechanisms. (houstonmethodist.org)
- Here we show that chronic and low level of arsenic stress induces transformation of the human bronchial epithelial cells, BEAS-2B, and that some of the transformed cells show characteristics of cancer stem-like cells (CSCs). (nih.gov)
- They produce progenitor cells that populate an area called the hair germ. (nih.gov)
- During hair growth, the progenitor cells differentiate (develop) into mature cells. (nih.gov)
- Existence of auditory and vestibular progenitor cells. (nih.gov)
- Identification of stem or progenitor cells that give rise to hair cells/supporting cells in embryos and adults (human). (nih.gov)
- Identification of new cell surface markers for progenitor cells using non-traditional probe generating techniques. (nih.gov)
- 1. Reprogramming of mesenchymal stem cells by oncogenes. (nih.gov)
- 7. Reprogramming of Oncogene Expression in Gingival Mesenchymal Stem Cells Following Long-Term Culture In Vitro. (nih.gov)
- 9. Enhancing the efficiency of direct reprogramming of human mesenchymal stem cells into mature neuronal-like cells with the combination of small molecule modulators of chromatin modifying enzymes, SMAD signaling and cyclic adenosine monophosphate levels. (nih.gov)
- 10. A genetic platform to model sarcomagenesis from primary adult mesenchymal stem cells. (nih.gov)
- 16. Senescence bypass in mesenchymal stem cells: a potential pathogenesis and implications of pro-senescence therapy in sarcomas. (nih.gov)
- Such β cell plasticity appears after nursing, independently of hyperglycaemia, and becomes more apparent along with diabetes progression accompanied with no significant increase in apoptosis. (diabetesjournals.org)
- In this study, we examined the potential effects of a GLP-1 receptor agonist on β cell plasticity and glucose homeostasis in young Wfs1 −/− mice without noticeable hyperglycaemia. (diabetesjournals.org)
- My lab also researches what underlies the remarkable ability of Schwann cells to regenerate and repair injury in the peripheral nervous system and how proteins such as c-Jun, Sox-2 and the Merlin tumour suppressor facilitate this repair and cell plasticity. (plymouth.ac.uk)
- Altogether, our findings highlight a unique mechanism of arsenic-induced transformation of human bronchial epithelial cells and provide a novel therapeutic target for arsenic-initiated lung cancer. (nih.gov)
- In addition to macrophages, which are traditionally assigned to this task, neighboring epithelial cells in the affected tissue are postulated to contribute to this process. (jci.org)
- Here we demonstrate that injured kidney epithelial cells assumed attributes of endogenous phagocytes. (jci.org)
- When a big amputation happens, those cells are essentially called upon to regenerate the missing structure, no matter what it is. (discovermagazine.com)
Neural crest progenitor2
- Furthermore, I had the opportunity to be a part of a collaboration with biophysicists, which led to the development of Spatial Genomic Analysis, a single molecule fluorescent in situ hybridization (smFISH)-based multiplex transcriptome analysis that revealed a pluripotent stem cell niche embedded in the neural crest progenitor domain in the dorsal neural tube. (nih.gov)
- Harvard researchers have engineered zebrafish that can be used to visually track melanoma as it begins - from its initiation from neural crest progenitor (NCP)-like cells to full-blown melanoma. (medscape.com)
- The team next took a closer look at the cells' structure and gene activity at different stages of hair growth. (nih.gov)
- The regenerative potential of two types of platelet-rich plasma (PRP), prepared in the presence of EDTA (EPRP) and citrate (CPRP) and an alternative blood product-hyperacute serum (hypACT) was evaluated using a 3D osteoarthritic chondrocyte pellet model by assessing the metabolic cell activity, cartilage-related gene expression and extracellular matrix deposition within the pellets. (mdpi.com)
- Each mammalian cell carries two copies of each gene, one inherited from the mother (on the maternal chromosome) and one inherited from the father (on the paternal chromosome). (justia.com)
- Furthermore, we have found that genetic ablation of Txnip in Wfs1 -/- mice inhibited β cell dedifferentiation, resulting in prevention of diabetes progression with preservation of β cell mass. (diabetesjournals.org)
- Thus, in the appropriate microenvironment, transit-amplifying cells dedifferentiate, becoming functional stem cells during tissue regeneration. (northwestern.edu)
- Even highly differentiated, pigment-producing McSCs could dedifferentiate into stem cells. (nih.gov)
- they do not use (only) stem cells, but mature osteoblasts, which dedifferentiate in respone to fractures. (uni-ulm.de)
- Loss of functional β cells results in a gradual progression of insulin insufficiency in Wolfram syndrome caused by recessive WFS1 mutations. (diabetesjournals.org)
- In association with the functional improvements, Ex-4 alleviated β cell dedifferentiation evidenced by a decrease of MafA and a Ngn3 emergence. (diabetesjournals.org)
- Acceptance of other cell sources for functional integration, and identification of molecules and agents that impact this. (nih.gov)
- HIRN's overall mission will be to support innovative and collaborative translational research to understand how human beta cells are lost in T1D, and to find innovative strategies to protect and replace functional beta cell mass in humans. (nih.gov)
- Starting in 2014 NIDDK will establish a new team science program, the Human Islet Research Network (HIRN), to help organize and support collaborative translational research related to the loss of functional beta cell mass in Type 1 Diabetes (T1D). (nih.gov)
- HIRN will be jointly supported by NIDDK and the type 1 diabetes special funding program, and its overall mission will be to better understand how human beta cells are lost in T1D, and to find innovative strategies to protect or replace functional beta cell mass in diabetic patients. (nih.gov)
- Senescence of the immune system function could interfere with transmissible spongiform encephalopathy pathogenesis in other ways as well, such as impairing migrating intestinal dendritic cells or complement pathways involved in complexing PrP Res to follicular dendritic cells. (cdc.gov)
- Importantly, using Cre-lox technology we could show that the pathway acts cell-autonomously within cardiomyocytes. (uni-ulm.de)
- Defects in either the complement pathway or follicular dendritic cells result in resistance to peripheral scrapie infection ( 7 , 8 ), and this resistance likely occurs for peripheral transmissible spongiform encephalopathy infections in general. (cdc.gov)
- Working jointly in the Kerosuo and Werner labs, I have embarked upon an exciting scientific adventure identifying novel ubiquitination pathway components involved in craniofacial bone and cartilage formation from neural crest cells. (nih.gov)
- The Stem Cell Scholars from Basic Research to Clinical Translation training program has the following four components to ensure its success and provide a great benefit both to the students and the community that our campus serves. (ca.gov)
- 19. Pharmacological mimicking of caloric restriction elicits epigenetic reprogramming of differentiated cells to stem-like self-renewal states. (nih.gov)
- We used an innovative one-class logistic regression (OCLR) machine-learning algorithm to extract transcriptomic and epigenetic feature sets derived from non-transformed pluripotent stem cells and their differentiated progeny. (nih.gov)
- PHF20 functions as a key epigenetic regulator of stem cell self-renewal and cellular reprogramming ( 10 ), and is abundantly expressed in several cancers ( 11 - 14 ). (frontiersin.org)
- CD19 has been targeted through genetically engineered chimeric-antigen receptor (CAR) T cells, and via bispecific CD3-CD19 antibodies, with reports of relapse via CD19 loss. (nih.gov)
- 20. Concise review: Induced pluripotent stem cells versus embryonic stem cells: close enough or yet too far apart? (nih.gov)
- They have a pluripotent population of stem cells that just hangs out in the body at all [times] and is sporadically replacing damaged cells. (discovermagazine.com)
- My postdoctoral work in Professor Marianne Bronner's laboratory demonstrated that neural crest cells, despite their transient nature, maintain a premigratory stem cell niche of self-renewing and pluripotent cells located in the dorsal neural tube. (nih.gov)
- Combined overexpression of four transcription factors promotes effector T cell dedifferentiation toward early phenotypes. (bvsalud.org)
- This progression from stem to progenitor to mature cells goes only one way. (nih.gov)
- His lab has also demonstrated the use of MDSC, as a cell-based tolerogenic therapy, to suppress unwanted immune responses, such as autoimmunity and graft-versus-host diseases (GVHD), while still retaining graft verse leukemia (GVL) function. (houstonmethodist.org)
- Characterization of Arsenic-Induced Cancer Stem-Like Cells. (nih.gov)
- Characterization of the extracellular matrix components that control the stability or expansion of hair cells. (nih.gov)
- Characterization of cell-cell interactions involved in stabilizing hair cell-support cell interactions. (nih.gov)
- My graduate work focused on characterization of regulatory functions in mouse neural stem cells. (nih.gov)
- Cancer stem cells (CSCs) have been widely recognized as a key feature of GBM ( 2 , 3 ). (frontiersin.org)
- However, the inhibition of actin polymerization could not block Schwann cell dedifferentiation. (jneurosci.org)
- As peripheral nerves develop, Schwann cells extend their processes into axon bundles to sort out an axon destined to be myelinated. (jneurosci.org)
- Then, Schwann cells obtain a one-to-one relationship with the axon and form myelin by spiraling their membrane around the axon ( Mirsky and Jessen, 1999 ). (jneurosci.org)
- These morphological changes of Schwann cells are likely to be mediated by the actin cytoskeleton, which plays a key role in cell shape changes in response to various stimuli. (jneurosci.org)
- Since cytoskeletal dynamics might be involved in the extensive morphological changes of Schwann cells during demyelination, we investigated the role of actin filaments in the disruption of SLI and subsequent myelin ovoid formation. (jneurosci.org)
- The PNS is myelinated by Schwann cells which ensheathe and myelinate the large caliber axons and allow rapid (saltatory) conduction of nerve impulses. (plymouth.ac.uk)
- Previous studies have shown that the exogenous overexpression of four essential factors (POU3F2, SOX2, SALL2, and OLIG2) is sufficient to fully reprogram differentiated GBM cells and induce poorly differentiated GBM stem-like cells ( 7 ). (frontiersin.org)
- Imprinting affects various essential cellular and developmental processes, including intercellular signaling, RNA processing, cell cycle control, and promotion or inhibition of cellular division and growth. (justia.com)
- I made this discovery by creating novel techniques such as developing crestospheres, an in vitro method to maintain neural crest stem cells in a self-renewing state. (nih.gov)
- Aim: The aim of this study is to identify CCs in various histological grades of oral squamous cell carcinoma (OSCC) and to relate them with the pattern of invasion, lymphocytic response (LR), and mitotic figures (Mfs). (who.int)
- I then continued as a Post Doctoral Fellow at the California Institute of Technology where I initiated my research on stem cell characteristics of the neural crest by adopting the early chicken embryo as my model system. (nih.gov)
- For unknow reasons, the melanocyte stem cell (McSC) system fails earlier than other adult stem cell populations 1 , which leads to hair greying in most humans and mice 2,3 . (nih.gov)
- Results: Four weeks administration of Ex-4 resulted in an off-drug amelioration of glucose excursions after glucose loading in Wfs1 −/− mice, with insulin secretory dynamics that were indistinguishable from those in WT mice, despite no alteration in beta cell mass. (diabetesjournals.org)
- Murine CD19 CAR treatment of ALL-bearing mice resulted in long-term remission with long persistence of T cells. (nih.gov)
- 8. Tumoral stem cell reprogramming as a driver of cancer: Theory, biological models, implications in cancer therapy. (nih.gov)
- However, cancer cells tend to follow anti-Dollo's law. (who.int)
- Unfavorable conditions such as hypoxia, acidic pH and low nutrients cause the cancer cells to switch their lifestyle atavistically in order to survive. (who.int)
- Recent work has highlighted the key role of dedifferentiation as a conserved mechanism for replenishing stem cell pools after their loss, thereby maintaining tissue homeostasis . (bvsalud.org)
- Following injury, the clearance of apoptotic and necrotic cells is necessary for mitigation and resolution of inflammation and tissue repair. (jci.org)
- Essentially, what that does is that they take whatever's remaining in the tissue, and they just shuffle the cells around, reorganize them, so that makes a perfectly formed mini-version with all of the appropriate structures," says McCusker. (discovermagazine.com)
- Disease-associated forms of resistant prion protein (PrP Res ) are likely transported from the gut to lymphoid tissue by cells such as migrating intestinal dendritic cells ( 6 ). (cdc.gov)
- Once in the lymphoid tissue PrP Res appears to be amplified by follicular dendritic cells ( 6 ) and then enters the nervous system. (cdc.gov)
- Establishment of human cell lines from embryonic ear tissue. (nih.gov)
- New findings enhanced our understanding of adult stem cells in ways that may enable researchers to expand their use for tissue and organ repair. (nih.gov)
- This invention relates generally to screening for risk or or presence of neoplastic disorders, and more particularly to screening for biomarkers present in a biological sample obtained from a subject that are indicative of a predisposition for a neoplastic (e.g., benign or malignant) or cell proliferative disorder. (justia.com)
- Meanwhile, the original stem cells remain as stem cells, separate from the progenitors. (nih.gov)
- Investigation of the otic placode as one possible beginning source to identify progenitors, although cells are already developmentally biased. (nih.gov)
- potential progenitors, supporting cells, etc. (nih.gov)
- Later relapses had loss of all the B cell surface markers, along with down regulation of B cell transcripts (such as EBF1 and PAX5) in the mRNA. (nih.gov)
- 5. How tumour cell identity is established? (nih.gov)