A reverse developmental process in which terminally differentiated cells with specialized functions revert back to a less differentiated stage within their own CELL LINEAGE.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A publication issued at stated, more or less regular, intervals.
A metabolic process that converts GLUCOSE into two molecules of PYRUVIC ACID through a series of enzymatic reactions. Energy generated by this process is conserved in two molecules of ATP. Glycolysis is the universal catabolic pathway for glucose, free glucose, or glucose derived from complex CARBOHYDRATES, such as GLYCOGEN and STARCH.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).
A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.
A subtype of DIABETES MELLITUS that is characterized by INSULIN deficiency. It is manifested by the sudden onset of severe HYPERGLYCEMIA, rapid progression to DIABETIC KETOACIDOSIS, and DEATH unless treated with insulin. The disease may occur at any age, but is most common in childhood or adolescence.
A cell surface receptor for INSULIN. It comprises a tetramer of two alpha and two beta subunits which are derived from cleavage of a single precursor protein. The receptor contains an intrinsic TYROSINE KINASE domain that is located within the beta subunit. Activation of the receptor by INSULIN results in numerous metabolic changes including increased uptake of GLUCOSE into the liver, muscle, and ADIPOSE TISSUE.
A heterogeneous group of disorders characterized by HYPERGLYCEMIA and GLUCOSE INTOLERANCE.
A subclass of DIABETES MELLITUS that is not INSULIN-responsive or dependent (NIDDM). It is characterized initially by INSULIN RESISTANCE and HYPERINSULINEMIA; and eventually by GLUCOSE INTOLERANCE; HYPERGLYCEMIA; and overt diabetes. Type II diabetes mellitus is no longer considered a disease exclusively found in adults. Patients seldom develop KETOSIS but often exhibit OBESITY.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A family of Urodela consisting of 15 living genera and about 42 species and occurring in North America, Europe, Asia, and North Africa.
Highly proliferative, self-renewing, and colony-forming stem cells which give rise to NEOPLASMS.
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
A cell line derived from cultured tumor cells.
Spherical, heterogeneous aggregates of proliferating, quiescent, and necrotic cells in culture that retain three-dimensional architecture and tissue-specific functions. The ability to form spheroids is a characteristic trait of CULTURED TUMOR CELLS derived from solid TUMORS. Cells from normal tissues can also form spheroids. They represent an in-vitro model for studies of the biology of both normal and malignant cells. (From Bjerkvig, Spheroid Culture in Cancer Research, 1992, p4)
Specialized stem cells that are committed to give rise to cells that have a particular function; examples are MYOBLASTS; MYELOID PROGENITOR CELLS; and skin stem cells. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from: http://www.nih.gov/news/stemcell/primer.htm)
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
An octamer transcription factor that is expressed primarily in totipotent embryonic STEM CELLS and GERM CELLS and is down-regulated during CELL DIFFERENTIATION.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
A naturally occurring phenomenon where terminally differentiated cells dedifferentiate to the point where they can switch CELL LINEAGES. The cells then differentiate into other cell types.
The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.
Stratified squamous epithelium that covers the outer surface of the CORNEA. It is smooth and contains many free nerve endings.
An annular transitional zone, approximately 1 mm wide, between the cornea and the bulbar conjunctiva and sclera. It is highly vascular and is involved in the metabolism of the cornea. It is ophthalmologically significant in that it appears on the outer surface of the eyeball as a slight furrow, marking the line between the clear cornea and the sclera. (Dictionary of Visual Science, 3d ed)
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells.
Obstruction of flow in biological or prosthetic vascular grafts.
The vessels carrying blood away from the capillary beds.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
The vein which drains the foot and leg.
An intermediate filament protein found in most differentiating cells, in cells grown in tissue culture, and in certain fully differentiated cells. Its insolubility suggests that it serves a structural function in the cytoplasm. MW 52,000.
The innermost layer of an artery or vein, made up of one layer of endothelial cells and supported by an internal elastic lamina.
The portion of chromosome material that remains condensed and is transcriptionally inactive during INTERPHASE.
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
Post-transcriptional biological modification of messenger, transfer, or ribosomal RNAs or their precursors. It includes cleavage, methylation, thiolation, isopentenylation, pseudouridine formation, conformational changes, and association with ribosomal protein.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Within most types of eukaryotic CELL NUCLEUS, a distinct region, not delimited by a membrane, in which some species of rRNA (RNA, RIBOSOMAL) are synthesized and assembled into ribonucleoprotein subunits of ribosomes. In the nucleolus rRNA is transcribed from a nucleolar organizer, i.e., a group of tandemly repeated chromosomal genes which encode rRNA and which are transcribed by RNA polymerase I. (Singleton & Sainsbury, Dictionary of Microbiology & Molecular Biology, 2d ed)
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed)
The excretory duct of the testes that carries SPERMATOZOA. It rises from the SCROTUM and joins the SEMINAL VESICLES to form the ejaculatory duct.
The nonstriated involuntary muscle tissue of blood vessels.
Non-striated, elongated, spindle-shaped cells found lining the digestive tract, uterus, and blood vessels. They are derived from specialized myoblasts (MYOBLASTS, SMOOTH MUSCLE).
The phenomenon by which dissociated cells intermixed in vitro tend to group themselves with cells of their own type.
A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments.

A genomic comparison of in vivo and in vitro brain microvascular endothelial cells. (1/372)

The blood-brain barrier (BBB) is composed of uniquely differentiated brain microvascular endothelial cells (BMEC). Often, it is of interest to replicate these attributes in the form of an in vitro model, and such models are widely used in the research community. However, the BMEC used to create in vitro BBB models de-differentiate in culture and lose many specialized characteristics. These changes are poorly understood at a molecular level, and little is known regarding the consequences of removing BMEC from their local in vivo microenvironment. To address these issues, suppression subtractive hybridization (SSH) was used to identify 25 gene transcripts that were differentially expressed between in vivo and in vitro BMEC. Genes affected included those involved in angiogenesis, transport and neurogenesis, and real-time quantitative polymerase chain reaction (qPCR) verified transcripts were primarily and significantly downregulated. Since this quantitative gene panel represented those BMEC characteristics lost upon culture, we used it to assess how culture manipulation, specifically BMEC purification and barrier induction by hydrocortisone, influenced the quality of in vitro models. Puromycin purification of BMEC elicited minimal differences compared with untreated BMEC, as assessed by qPCR. In contrast, qPCR-based gene panel analysis after induction with hydrocortisone indicated a modest shift of 10 of the 23 genes toward a more 'in vivo-like' gene expression profile, which correlated with improved barrier phenotype. Genomic analysis of BMEC de-differentiation in culture has thus yielded a functionally diverse set of genes useful for comparing the in vitro and in vivo BBB.  (+info)

Retinoic acid inhibits Th17 polarization and enhances FoxP3 expression through a Stat-3/Stat-5 independent signaling pathway. (2/372)

CD4(+) helper T (Th) cells play a crucial role in the delicate balance between host defense and autoimmune disease. Two important populations of helper T cells are the proinflammatory, interleukin-17 (IL-17)-producing (Th17) cells and the anti-inflammatory forkhead box P3-positive (FoxP3(+)) T regulatory (Treg) cells. Here we show that all-trans retinoic acid (ATRA) and other agonists of the retinoic acid receptor alpha (RARalpha) inhibit the formation of Th17 cells and promote FoxP3 expression. Conversely, inhibition of retinoic acid signaling constrains transforming growth factor beta (TGF-beta1) induction of FoxP3. The effect of ATRA is mediated independently of IL-2, signal transducer and activator of transcription 5 (Stat5) and Stat3, representing a novel mechanism for the induction of FoxP3 in CD4 T cells. As previous studies have shown that vitamin A derivatives are protective in animal models of autoimmune disease, the current data suggest a previously unrecognized role for RARalpha in the regulation of CD4(+) T-cell differentiation and provide a mechanism for the anti-inflammatory effects of retinoic acid.  (+info)

Aromatic residues in the C-terminal domain 2 are required for Nanog to mediate LIF-independent self-renewal of mouse embryonic stem cells. (3/372)

Nanog was identified by its ability to sustain the LIF-independent self-renewal of mouse embryonic stem (ES) cells and has recently been shown to play a role in reprogramming adult fibroblasts into pluripotent stem cells. However, little is known about the structural basis of these remarkable activities of Nanog. We have previously identified an unusually strong transactivator named CD2 at its C terminus. Here we demonstrate that CD2 is required for Nanog to mediate ES cell self-renewal. Furthermore, deletion and point mutation analysis revealed that CD2 relies on at least seven aromatic amino acid residues to generate its potent transactivating activity. A mutant Nanog bearing alanine substitutions for these seven residues fails to confer LIF-independent self-renewal in mouse ES cells. Substitution of CD2 by the viral transactivator VP16 gave rise to Nanog-VP16, which is 10 times more active than wild-type Nanog in ES cells. Surprisingly, the expression of Nanog-VP16 in mouse ES cells induces differentiation and is thus unable to sustain LIF-independent self-renewal for mouse ES cells. Taken together, our results demonstrate that the CD2 domain of Nanog is a unique transactivator that utilizes aromatic residues to confer specific activity absolutely required for ES self-renewal.  (+info)

Epithelial-to-mesenchymal transition is a potential pathway leading to podocyte dysfunction and proteinuria. (4/372)

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Functional network reconstruction reveals somatic stemness genetic maps and dedifferentiation-like transcriptome reprogramming induced by GATA2. (5/372)

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A developmental model of sarcomagenesis defines a differentiation-based classification for liposarcomas. (6/372)

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Diffusion-weighted MR imaging: diagnosing atypical or malignant meningiomas and detecting tumor dedifferentiation. (7/372)

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Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. (8/372)

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TY - THES. T1 - Study of endocrine markers involved in dedifferentiation and identification of C function or redifferentiate pancreatic β-cells in type 2 diabetes. AU - Neelankal John, Abraham. PY - 2018. Y1 - 2018. N2 - To enhance the knowledge of β-cell dedifferentiation (BCD) and its causal role in Type 2 diabetes (T2D), my studies employed β-cell line mouse insulinoma 6 (MIN6) and db/db islets from T2D model mice thereby establishing those as models of dedifferentiation. Vitamin D receptor (VDR)-targeted therapy that employed LCA propionate was utilized to treat early passage MIN6 and db/+ mice islets in the pre-diabetic state to protect β-cell from undergoing dedifferentiation. Furthermore, my research using MIN6 and db/db also identified a novel druggable compound ANJR12947285, which has the potential to induce redifferentiation in dedifferentiated β-cell. AB - To enhance the knowledge of β-cell dedifferentiation (BCD) and its causal role in Type 2 diabetes (T2D), my studies employed ...
This investigation reveals that human vein grafts very soon after CABG show distinct changes. These changes are characterised by loss of surface lining endothelial cells, insudation of blood corpuscular elements such as polymorphonuclear leucocytes and monocytes, admixed with a fibrin-platelet thrombus, the appearance of vimentin positive, actin negative spindle shaped cells, and absence of actin positive SMCs in the pre-existent media topographically related to the sites of neointimal reaction. These observations allow speculations as to the mechanisms involved in neointimal thickening.. Denudation of the surface endothelial cell lining appears to be a crucial factor, and its extent and severity may eventually determine graft patency. Loss of endothelial cells, as observed in veins 2-9 days after grafting, is accompanied by insudation with polymorphonuclear leucocytes, blood monocytes, and T lymphocytes. To this end our observations fit with previous experimental works. Brody and colleagues11 ...
https://doi.org/10.18632/oncotarget.15888 Pan Wang, Chuan Lan, Shuanglong Xiong, Xiuwen Zhao, Youan Shan, Rong Hu, Wenwu Wan, Shuangjiang Yu, Bin Liao, Guangzhi Li, Junwei Wang, Dewei Zou, Bing...
Background-The mechanisms underlying the de-differentiation and lineage conversion of adult human fibroblasts into functional endothelial cells have not yet been fully defined. Furthermore, it is not known whether fibroblast de-differentiation recapitulates the generation of multipotent progenitors during embryonic development which give rise to endothelial and hematopoietic cell lineages. Here we established the role of the developmental transcription factor SOX17 in regulating the bi-lineage conversion of fibroblasts via the generation of intermediate progenitors.. Methods-CD34+ progenitors were generated following the de-differentiation of human adult dermal fibroblasts by overexpression of pluripotency transcription factors. Sorted CD34+ cells were transdifferentiated into induced endothelial cells (iECs) and induced erythroblasts (iErythroblasts) using lineage specific growth factors. The therapeutic potential of the generated cells was assessed in an experimental model of myocardial ...
One of the most remarkable properties of plants is their capacity to regenerate tissue structures and even whole organs to replace those damaged or lost through injury. Plants are able to do this thanks to high-level dedifferentiation, a process whereby mature cells withdraw from their specialized state and acquire proliferation ability and pluripotency, enabling them to develop anew into different cell types. While the knowledge and use of techniques for plant organ regeneration has a long history in horticulture, little is known about the molecular mechanisms underlying dedifferentiation.. To clarify these mechanisms, the researchers studied a common type of cell dedifferentiation induced by wounding, where its role in tissue and organ regeneration is critical to survival. In plants, this regeneration frequently occurs through the creation of masses of cells known as callus, which grow over the wound to protect it. Using data from earlier research, the researchers identified a gene in the ...
Inhibitor of DNA Binding 4 (ID4) is a member of the helix-loop-helix ID family of transcription factors, mostly present in the central nervous system during embryonic development, that has been associated with TP53 mutation and activation of SOX2. Along with other transcription factors, ID4 has been implicated in the tumorigenic process of astrocytomas, contributing to cell dedifferentiation, proliferation and chemoresistance. In this study, we aimed to characterize the ID4 expression pattern in human diffusely infiltrative astrocytomas of World Health Organization (WHO) grades II to IV of malignancy (AGII-AGIV); to correlate its expression level to that of SOX2, SOX4, OCT-4 and NANOG, along with TP53 mutational status; and to correlate the results with the clinical end-point of overall survival among glioblastoma patients. Quantitative real time PCR (qRT-PCR) was performed in 130 samples of astrocytomas for relative expression, showing up-regulation of all transcription factors in tumor cases. ...
In addition to being a component of innate immunity and an ancient defense mechanism against invading pathogens, complement activation also participates in the adaptive immune response, inflammation, hemostasis, embryogenesis, and organ repair and development. Activation of the complement system via classical, lectin, or alternative pathways generates anaphylatoxins (C3a and C5a) and membrane attack complex (C5b-9) and opsonizes targeted cells. Complement activation end products and their receptors mediate cell-cell interactions that regulate several biological functions in the extravascular tissue. Signaling of anaphylatoxin receptors or assembly of membrane attack complex promotes cell dedifferentiation, proliferation, and migration in addition to reducing apoptosis. As a result, complement activation in the tumor microenvironment enhances tumor growth and increases metastasis. In this Review, I discuss immune and nonimmune functions of complement proteins and the tumor-promoting effect of ...
In addition to being a component of innate immunity and an ancient defense mechanism against invading pathogens, complement activation also participates in the adaptive immune response, inflammation, hemostasis, embryogenesis, and organ repair and development. Activation of the complement system via classical, lectin, or alternative pathways generates anaphylatoxins (C3a and C5a) and membrane attack complex (C5b-9) and opsonizes targeted cells. Complement activation end products and their receptors mediate cell-cell interactions that regulate several biological functions in the extravascular tissue. Signaling of anaphylatoxin receptors or assembly of membrane attack complex promotes cell dedifferentiation, proliferation, and migration in addition to reducing apoptosis. As a result, complement activation in the tumor microenvironment enhances tumor growth and increases metastasis. In this Review, I discuss immune and nonimmune functions of complement proteins and the tumor-promoting effect of ...
What is the source of your epithelial cell? The morphology cannot certify that a cell is undergoing de-differentiation. You have to show something biochemically (immunostaining, PCR ,western) to show that the cell shuttles between two differentiated adult kind to prove dedifferentiation ...
The ability to dedifferentiate stem cells (the article uses the term retrodifferentiation) would be incredibly valuable just for leukemia treatment. Though if all their claims are correct the number of applications would be enormous. TriStem hasnt yet proven all their claims and if you read the full article you will find different scientists voicing varying degrees of skepticism about those claims. But TriStem has begun to demonstrate some of their claims to outside scientists including Tim McCaffrey of George Washington University and a clinical trial in Britain will attempt to use TriStems technique to treat aplastic anemia with results due by March 2004. So we will soon know a lot more about their claims.. The ability to use a persons own blood cells, dedifferentiate them and to grow them in large number for conversion into a wide variety of cell types would provide the advantages of hESC while avoiding political opposition in the form of the types of ethical objections which have been ...
In this weeks Editors Pick, Jayaraj Rajagopal and colleagues, describe their findings studying the ability of mouse airway epithelia to dedifferentiate and contribute stem cells for the adult airway. This study may be part of a more generalizable mechanism whereby differentiated cells may dedifferentiate and contribute to the regenerative ability of different organs.. ...
The injection into athymic nude mice of well-differentiated cells of the H4IIEC3 rat hepatoma line leads to tumor take and growth. Animals given injections of cells of a dedifferentiated variant subclone, however, do not develop tumors, whereas revertant clones are malignant. Nevertheless, tumors are obtained by increasing the number of injected dedifferentiated cells, and the cells from these tumors do express liver-specific messenger RNAs. Finally, the differentiated state of these tumor cells is stable in vitro. This correlation between the differentiated state of the cells and tumorigenicity is also observed in somatic hybrids between the variant cells and the differentiated ancestors. These hybrids express the hepatic functions and give rise to tumors. The only in vitro character of transformation which distinguishes the two types of cells is anchorage independence of growth. Since two other independent variants develop tumors, it is established that the nonmalignant state is not simply ...
2 For the people of these multiple conditions the penalties of this download starship are widely rapid with DFAT from 1 July 2013. The parameters now at 1 July 2013 accredited by DFAT and required by resources are the students and opportunities of rates as at 30 June 2013. All download starship and people composite to this Property have encountered as case of DFAT in 2013-14.
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Diabetes is caused by a combination of impaired responsiveness to insulin and reduced production of insulin by the pancreas. Until recently, the decline of insulin production had been ascribed to β-cell death. But recent research has shown that β-cells do not die in diabetes, but undergo a silencing …
MAIN RESULTS: We identified two RCTs and one CCT (total number of participants 149) evaluating the use of amifostine versus no additional treatment in the original version of the review; the updates identified no additional studies. Two studies included children with osteosarcoma, and the other study included children with hepatoblastoma. Children received cisplatin only or a combination of cisplatin and carboplatin, either intra-arterially or intravenously. Pooling of results of the included studies was not possible. From individual studies the effect of amifostine on symptomatic ototoxicity only (i.e. National Cancer Institute Common Toxicity Criteria version 2 (NCICTCv2) or modified Brock grade 2 or higher) and combined asymptomatic and symptomatic ototoxicity (i.e. NCICTCv2 or modified Brock grade 1 or higher) were uncertain (low-certainty evidence). Only one study including children with osteosarcoma treated with intra-arterial cisplatin provided information on tumour response, defined as ...
Purpose: : To investigate possible mechanism involved in the de-differentiation of human corneal epithelial cells in a defined low calcium and serum free medium Methods: : Human corneal epithelial cells were cultivated from fresh limbal tissue explants in two conditions: high calcium serum-containing SHEM and a defined, low calcium and serum free medium (CnT-20). After 2 weeks, they were passaged and seeded at two densities, low and high, in CnT-20 medium. Colony forming efficiency without any feeder layer was quantified in low density condition. Subsequent passages were performed for cells seeded at high density. Gene expression was analyzed by reverse transcription and real time PCR with TaqMan primers and probes. Protein localization was determined by immunofluorescent staining on corneoscleral tissue cryosections and in cell cultures. Results: : Corneal epithelial cells harvested from explant cultures in the low calcium serum-free defined medium CnT-20 generated 2 fold colony forming ...
Title: Molecular Mechanisms Underlying the Dedifferentiation Process of Isolated Hepatocytes and Their Cultures. VOLUME: 7 ISSUE: 6. Author(s):Greetje Elaut, Tom Henkens, Peggy Papeleu, Sarah Snykers, Mathieu Vinken, Tamara Vanhaecke and Vera Rogiers. Affiliation:Vrije Universiteit Brussel, Department of Toxicology, Laarbeeklaan 103, B-1090 Brussels, Belgium.. Keywords:Hepatocyte, isolation, cultivation, dedifferentiation, apoptosis, proliferation, ischemia-reperfusion injury, cell-cell interactions, cell-extracellular matrix interactions. Abstract: Primary hepatocytes and their cultures are a simple but versatile, well-controlled, and relatively easy to handle in vitro system that is well-accepted for investigating xenobiotic biotransformation, enzyme induction and inhibition, and (biotransformation-mediated) hepatotoxicity. In addition, hepatocyte cultures have proven to be valuable tools in the study of liver physiology, viral hepatitis, and liver regeneration and are proposed as an ...
Dermal adipose tissue (also known as dermal white adipose tissue and herein referred to as dWAT) has been the focus of much discussion in recent years. However, dWAT remains poorly characterized. The fate of the mature dermal adipocytes and the origin of the rapidly reappearing dermal adipocytes at different stages remain unclear. Here, we isolated dermal adipocytes and characterized dermal fat at the cellular and molecular level. Together with dWATs dynamic responses to external stimuli, we established that dermal adipocytes are a distinct class of white adipocytes with high plasticity. By combining pulse-chase lineage tracing and single-cell RNA sequencing, we observed that mature dermal adipocytes undergo dedifferentiation and redifferentiation under physiological and pathophysiological conditions. Upon various challenges, the dedifferentiated cells proliferate and redifferentiate into adipocytes. In addition, manipulation of dWAT highlighted an important role for mature dermal adipocytes ...
Loss of switch/sucrose non-fermenting complex protein expression is associated with dedifferentiation in endometrial carcinomas Academic Article ...
Read Functional dedifferentiation and reduced task-related deactivations underlie the age-related decline of prospective memory, Brain Imaging and Behavior on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Figure 2: SSEA-4 is expressed in cells of human testis that have an active POU5F1 promoter. (A): Stably transfected human germ cells with a GFP reporter plasmid under the control of the POU5F1 promoter. Cells expressing GFP (green, shown by the arrows) were used to assay the expression of SSEA-4 in (B). Nuclei are stained by DAPI (blue). Scale bars = 5μm. (B): qRT-PCR analysis of the expression of POU5F1, cMYC and SSEA-4 genes in the following cell populations: Testis is whole human testis cell suspension, SSCs are the POU5F1 promoter-GFP expressing cells cultured under SSC maintenance conditions, gPS are the germline pluripotent cells generated when the GFP expressing cells are cultured under de-differentiation conditions for 5 weeks, iPSCs are the IMR90-4 induced pluripotent stem cells used as a control. The different samples were assayed in triplicates and normalized to GAPDH. Asterisks show significant differences in mRNA levels, as determined by t-test analysis (p,0.05 ...
Tumor progression often coincides with the appearance of tumor cells with dedifferentiated or stem-like properties; the ontogeny of these cells is unclear, but they are thought to carry a more malignant capacity than their differentiated counterparts. Cdx2 is a transcriptional factor that specifies cell fate of the intestinal epithelium. Epigenetic studies have demonstrated that Cdx2 loss alters chromatin structure in the intestinal epithelium. Cdx2 loss is often observed during colon tumor progression and is correlated with de-differentiation. Our current study is aimed to test the hypothesis that the Cdx2 prevents tumor progression by maintaining the tumor epigenome. To this end, we have generated two mouse models to determine the effect of Cdx2 loss on chromatin structure in a tumorigenic environment and in primary tumors. Our current studies are aimed at comparing Cdx2 binding sites on the genome in a normal and tumorigenic environment, and in defining how Cdx2 loss affects chromatin ...
The proximal tubule has a remarkable capacity for repair after acute injury, but the cellular lineage and molecular mechanisms underlying this repair response are incompletely understood. Here, we developed a Kim1-GFPCreERt2 knockin mouse line (Kim1-GCE) in order to perform genetic lineage tracing of dedifferentiated cells while measuring the cellular transcriptome of proximal tubule during repair. Acutely injured genetically labeled clones coexpressed KIM1, VIMENTIN, SOX9, and KI67, indicating a dedifferentiated and proliferative state. Clonal analysis revealed clonal expansion of Kim1+ cells, indicating that acutely injured, dedifferentiated proximal tubule cells, rather than fixed tubular progenitor cells, account for repair. Translational profiling during injury and repair revealed signatures of both successful and unsuccessful maladaptive repair. The transcription factor Foxm1 was induced early in injury, was required for epithelial proliferation in vitro, and was dependent on epidermal ...
Current Research and Scholarly InterestsI am a cell and molecular biologist by training. During my PhD I worked on the identification of thymidine kinase 1 phosphorylation status during cell cycle progression and its relevance for PET imaging of cell proliferation. In the Stearns lab I was interested in how cells cope with multiple centrosomes and what are the mechanisms ensuring centrosome number homeostasis. In the Sebastiano lab I am studying germ cell differentiation and what are the cell biological effects of de-differentiation. ...
Several studies have demonstrated age-related regional differences in the magnitude of the BOLD signal using task-based fMRI. It has been suggested that functional changes reflect either compensatory or de-differentiation mechanisms, both of which assume response to a specific stimulus. Here, we have tested whether ageing affects both task-based and resting brain function, and the extent to which functional changes are mediated by reductions in grey matter (GM) volume. Two groups, of 22 healthy younger and 22 older volunteers, underwent an imaging protocol involving structural and functional MRI, both during a memory task and at rest. The two groups had similar socio-demographical characteristics and cognitive performance. Image analysis revealed both structural and functional differences. Increased BOLD signal in older relative to younger volunteers was mainly observed in the frontal lobes, both during the task and at rest. Functional changes in the frontal lobes were largely located in brain regions
in Glia (2017), 65(10), 1682-1696. The molecular mechanisms that regulate Schwann cell (SC) plasticity and the role of the Nrg1/ErbB-induced MEK1/ERK1/2 signalling pathway in SC dedifferentiation or in myelination remain unclear. It is ... [more ▼]. The molecular mechanisms that regulate Schwann cell (SC) plasticity and the role of the Nrg1/ErbB-induced MEK1/ERK1/2 signalling pathway in SC dedifferentiation or in myelination remain unclear. It is currently believed that different levels of MEK1/ERK1/2 activation define the state of SC differentiation. Thus, the identification of new regulators of MEK1/ERK1/2 signalling could help to decipher the context-specific aspects driving the effects of this pathway on SC plasticity. In this perspective, we have investigated the potential role of KIAA1199, a protein that promotes ErbB and MEK1/ERK1/2 signalling in cancer cells, in SC plasticity. We depleted KIAA1199 in the SC-derived MSC80 cell line with RNA-interference-based strategy and also generated ...
All of the known driver genes can be classified into one or more of 12 pathways (Fig. 7). The discovery of the molecular components of these pathways is one of the greatest achievements of biomedical research, a tribute to investigators working in fields that encompass biochemistry, cell biology, and development, as well as cancer. These pathways can themselves be further organized into three core cellular processes:. 1) Cell fate: Numerous studies have demonstrated the opposing relationship between cell division and differentiation, the arbiters of cell fate. Dividing cells that are responsible for populating normal tissues (stem cells) do not differentiate, and vice versa. Regenerative medicine is based on this distinction, predicated on ways to get differentiated cells to dedifferentiate into stem cells, then forcing the stem cells to differentiate into useful cell types for transplantation back into the patient. Many of the genetic alterations in cancer abrogate the precise balance between ...
In PNAS this week: method to construct ultrahigh-density linkage map using population sequencing, different functions of hoxa3a orthologues in mice and zebrafish, alternative splicing and ESC pluripotency, and more.
Affiliation (Current):鹿児島大学,医歯学総合研究科,客員研究員, Research Field:Dental engineering/Regenerative dentistry,Periodontal dentistry,Surgical dentistry, Keywords:歯周組織再生,DFAT,骨,再生,歯周病,インプラント周囲炎,骨再生,骨組織,骨補填材,β-TCP, # of Research Projects:7, # of Research Products:17
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TY - JOUR. T1 - 170 Mitochondrial-dependent signalling in vascular smooth muscle cell proliferation. AU - Al-Sulti, Zuhair. AU - Kingsmore, David. AU - Coats, Paul. PY - 2014/6. Y1 - 2014/6. N2 - UNLABELLED: A hallmark of vascular disease is the cellular adaptive response characterised by proliferation and migration. Although many studies have identified the signalling pathways involved in cell proliferation and migration (p38, p44/42 MAP Kinase and JNK), the mechanisms initiating cell de-differentiation, proliferation/ apoptosis and migration are yet to be fully elucidated. Mitochondria are one of the organelles that have received growing attention in vascular and pulmonary vascular proliferative disease.(1) Mitochondria are classically known to be responsible for cellular energy production. However growing evidence suggests a role in cell de-differentiation and the fine balance between cell apoptosis and proliferation.(2) The aim of this work was to expand our understanding of the potential ...
7.3 The letter of support should attach relevant supporting documentation including the letter of offer from the employer, proof of health insurance and, where applicable, health and character checks. 7.4 A request will not be considered by DFAT Australia until all eligibility criteria specified in paragraph 7 .2 of this MoU have been satisfied. If any of the provisions of 7.2 of this MoU are not satisfied, this will constitute grounds for DFAT Australia to refuse the request. 7.5 If DFAT Australia has no objection to a request, DFAT Australia will advise DFAT Nauru accordingly and provide a letter of support. An application for a Subclass 403 (Temporary Work (International Relations) visa or its equivalent should then be submitted to the relevant visa processing office in Suva for processing by DIBP. Applications will be made on private passports and a Visa Application Charge may apply. 7.6 Visas issued pursuant to this MoU will have conditions attached to them and the Nominee will be subject ...
Tumors have characteristics that allow determination of malignancy and how they will evolve, and determining these characteristics will allow the medical team to determine the management plan. Anaplasia or dedifferentiation: loss of differentiation of cells and of their orientation to one another and blood vessels, a characteristic of anaplastic tumor tissue. Anaplastic cells have lost total control of their normal functions and many have deteriorated cell structures. Anaplastic cells often have abnormally high nuclear-to-cytoplasmic ratios, and many are multinucleated. Additionally, the nuclei of anaplastic cells are usually unnaturally shaped or oversized. Cells can become anaplastic in two ways: neoplastic tumor cells can dedifferentiate to become anaplasias (the dedifferentiation causes the cells to lose all of their normal structure/function), or cancer stem cells can increase their capacity to multiply (i.e., uncontrollable growth due to failure of differentiation). Atypia: an indication ...
In cancer, epithelial cell de-differentiation is a feature of rapidly dividing cells under non-controlled growth and it often reflects a change in the gene expression pattern; however, the relationship between proliferation and alterations in cellular differentiation has not yet been identified. This work examined how changes in the characteristics of cells that discriminate their differentiated and proliferative states can be used to improve on current pancreatic cancer chemotherapeutic strategies. PepT1, a high substrate-capacity and low-affinity transporter system, has been suggested as an attractive drug delivery target for pancreatic cancer. Through a combination of immunological assays, PepT1 normally restricted to the apical surfaces in polarised intestinal epithelial cells, was shown to distribute at the cell membrane of non-polarised cancerous ductal cells. Anti-inflammatory or anti-cancer agents, like ibuprofen or gemcitabine, were conjugated to selected amino acids to enhance their ...
Fully differentiated mature smooth muscle cells (SMCs) are characterized by the presence of a unique repertoire of contractile proteins. The expression of these proteins is markedly attenuated during the de-differentiation of smooth muscle that occurs under many pathological conditions. SRF is a transcription factor that plays a central role in the expression of these smooth muscle-specific genes through physical association with various cell-restricted or signal dependent accessory factors. Of the SRF-associated proteins identified, myocardin is the most potent for stimulating expression of smooth muscle-specific genes. Although several proteins have been identified that can modify myocardin function, the mechanism of how myocardin regulation is still poorly understood. To identify additional myocardin associated proteins that are required for myocardin-dependent activation smooth muscle-specific genes we performed a yeast 2-hybrid screen of mouse embryo cDNA library using myocardin as bait. ...
Current Research and Scholarly Interests I am a cell and molecular biologist by training. During my PhD I worked on the identification of thymidine kinase 1 phosphorylation status during cell cycle progression and its relevance for PET imaging of cell proliferation. In the Stearns lab I was interested in how cells cope with multiple centrosomes and what are the mechanisms ensuring centrosome number homeostasis. In the Sebastiano lab I am studying germ cell differentiation and what are the cell biological effects of de-differentiation. ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Direct Reprogramming Science Project: Investigate how transcription factors can be used to turn one cell type directly into another cell type, and why this technique is important for the future of the field of regenerative medicine.
Research outputs, collaborations and relationships for UiO Centre for Cancer Cell Reprogramming (CanCell) published between 1 December 2019 - 30 November 2020 as tracked by the Nature Index.
Representatives of the Indian Ocean Rim Association (IORA) met in Bali this week to discuss regional cooperation on the blue economy. Australia is the current chair of IORA and hosted this event. The blue economy is sustainable economic activity associated with the ocean and is a key driver of growth and job creation for economies in the Indian Ocean rim. ...
Scientists from Salk Institute for Biological Studies showed that One clue to halting or reversing aging lies in the study of cellular reprogramming, a p...
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செமினி நுண்மங்கள் தனது நகலாக்கத்திற்கு பயிர் பொறிகளை (machinery) பயன்படுத்துகின்றன. இவை சில மரபணுவை வெளிபடுத்தவதோடு அல்லாமல், அவற்றைக் கொண்டு மிக நேர்த்தியாக பயிர்களின் மரபணுவை பயன்படுத்தி பல்கி பெருகுகின்றன. மேலும் நுண்மங்களின் மரபணு முதிர்ந்த இலைகளின் உயிரணுவில் செயலற்ற மூலக்கூற்று நிகழ்வினை , மறு- நிகழ்வு அல்லது மறு-வினைக்கு (cell reprogramming) உட்படுத்தி நகலாக்கம் ...
Still a continuation of my Davao Food Appreciation Tour (DFAT) experience. Ive been hearing about Lachis from other bloggers for years. They all rave about … Read the rest. ...
It is followed by the dedifferentiation of the cells. This process produces only a minimal concentration of the cells. Hence the use of micro carrier cultures ...
TY - JOUR. T1 - Altered glycosylation associated with dedifferentiation of hepatocellular carcinoma. T2 - A lectin microarray-based study. AU - Takayama, Hiroomi. AU - Ohta, Masayuki. AU - Iwashita, Yukio. AU - Uchida, Hiroki. AU - Shitomi, Yuki. AU - Yada, Kazuhiro. AU - Inomata, Masafumi. N1 - Publisher Copyright: © 2020 The Author(s). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.. PY - 2020/3/6. Y1 - 2020/3/6. N2 - Background: Altered glycosylation associated with hepatocellular carcinoma (HCC) is well documented. However, few reports have investigated the association between dedifferentiation and glycosylation. Therefore, the aim of this study was to analyze glycosylation associated with dedifferentiation of HCC within the same nodule and to investigate glycosyltransferase related to the glycosylation. Methods: We analyzed resected HCC specimens (n = 50) using lectin microarray to comprehensively and sensitively analyze glycan profiles, and identify changes to glycosylation ...
Smooth muscle from newborn guinea-pig vas deferens was enzymically dispersed into single cells or small clumps and grown in culture in the presence or absence of sympathetic ganglion explants.. Most single smooth muscle cells gradually lost their typical ultrastructural features and contractile properties during the first few days in culture. At 7 days of culture these dedifferentiated smooth muscle cells underwent extensive proliferation. If sufficient cells were present in the culture inoculate, a continuous monolayer formed at about 9 days of culture and redifferentiation of smooth muscle began. At 11-12 days of culture the cells reaggregated into clumps, began to contract spontaneously, and formed into well-organized muscle bundles in two layers at right angles, resembling the muscle layer organization of the in vivo vas deferens. In cultures where a continuous monolayer was not formed at 9 days, isolated cells did not redifferentiate. The process of dedifferentiation and proliferation was ...
TY - JOUR. T1 - Chordoma dedifferentiation after proton beam therapy. T2 - a case report and review of the literature. AU - Frankl, Joseph. AU - Grotepas, Cassi. AU - Stea, Baldassarre. AU - Lemole, Gerald M. AU - Chiu, Alexander G. AU - Khan, Rihan. PY - 2016/10/12. Y1 - 2016/10/12. N2 - Background: Chordoma is a rare invasive bone tumor that may occur anywhere along the neuraxis. A total of three primary histological varieties have been identified: conventional, chondroid, and dedifferentiated. Case presentation: We report a case of an 8-year-old white girl who presented with conventional chordoma, was treated with surgical resection and mixed proton and photon beam therapy, and had a recurrence in the resection cavity 2.5 years later with dedifferentiated morphology. The recurrent tumor did not express brachyury, a recently identified protein specific to tissue of notochordal origin. Conclusions: The short time period between radiation therapy and dedifferentiation, low dose of photons, and ...
Endothelial progenitor cell (EPC) transplantation is a promising therapy for ischemic diseases such as ischemic myocardial infarction and hindlimb ischemia. However, limitation of EPC sources remains a major obstacle. Direct reprogramming has become a powerful tool to produce EPCs from fibroblasts. Some recent efforts successfully directly reprogrammed human fibroblasts into functional EPCs; however, the procedure efficacy was low. This study therefore aimed to improve the efficacy of direct reprogramming of human fibroblasts to functional EPCs. Human fibroblasts isolated from foreskin were directly reprogrammed into EPCs by viral ETV2 transduction. Reprogramming efficacy was improved by culturing transduced fibroblasts in hypoxia conditions (5 % oxygen). Phenotype analyses confirmed that single-factor ETV2 transduction successfully reprogrammed dermal fibroblasts into functional EPCs. Hypoxia treatment during the reprogramming procedure increased the efficacy of reprogramming from 1.21 ± 0.61 % in
The dramatic discovery that somatic cells could be reprogrammed to induced pluripotent stem cells (iPSCs), by the expression of just four factors, has opened new opportunities for regenerative medicine and novel ways of modeling human diseases. Extensive research over the short time since the first iPSCs were generated has yielded the ability to reprogram various cell types using a diverse range of methods. However the duration, efficiency, and safety of induced reprogramming have remained a persistent limitation to achieving a robust experimental and therapeutic system. The field has worked to resolve these issues through technological advances using non-integrative approaches, factor replacement or complementation with microRNA, shRNA and drugs. Despite these advances, the molecular mechanisms underlying the reprogramming process remain poorly understood. Recently, through the use of inducible secondary reprogramming systems, researchers have now accessed more rigorous mechanistic experiments to
Analysis of Beta-Cell Gene Expression Reveals Inflammatory Signaling and Evidence of Dedifferentiation following Human Islet Isolation and Culture. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
PubMedID: 22703534 | Nuclear factor erythroid 2-related factor 2 nuclear translocation induces myofibroblastic dedifferentiation in idiopathic pulmonary fibrosis. | Antioxidants & redox signaling | 1/1/2013
Chondrocytes in articular cartilage normally exhibit high expression of collagen II and aggrecan but rapidly dedifferentiate to a fibroblastic phenotype if passaged in culture. Previous studies have suggested that the loss of chondrocyte phenotype is associated with changes in the structure of the F-actin cytoskeleton, which also controls cell mechanical properties. In this study, we examined how dedifferentiation in monolayer influences the mechanical properties of chondrocytes isolated from different zones of articular cartilage. Atomic force microscopy was used to measure the mechanical properties of superficial and middle/deep zone chondrocytes as they underwent serial passaging and subsequent growth on fibronectin-coated, micropatterned self-assembled monolayers that restored a rounded cell shape in 2D culture. Chondrocytes exhibited significant increases in elastic and viscoelastic moduli with dedifferentiation in culture. These changes were only partially ameliorated by the restoration of ...
Pancreatic ductal adenocarcinoma (PDAC) is the third leading cause of cancer death in North America. The highest risk factor for PDAC is recurrent pancreatitis. While the link between PDAC and pancreatitis is unknown, de-differentiation of acinar cells is common to both diseases. Our lab has shown that Activating Transcription Factor 3 (ATF3), a factor upregulated during pancreatic injury, contributes to the development of acinar-to-ductal cell metaplasia (ADM), a precursor phenotype of PDAC. The goal of this study was to identify how ATF3 contributes to ADM. I hypothesize that ATF3 regulates acinar gene expression promoting ADM. We observed decreased ADM development in Atf3-/- acinar cultures, along with expression changes in differentiation genes and ADM promoting pathways (EGFR) in vivo. Assessment following chronic injury indicated absence of ATF3 resulted in decreased tissue damage. These results suggest a novel mechanism where ATF3 promotes ADM through loss of the mature acinar cell phenotype.
Human induced pluripotent stem cells (hiPSCs) have been generated by reprogramming a number of different somatic cell types using a variety of approaches. In addition, direct reprogramming of mature cells from one lineage to another has emerged recently as an alternative strategy for generating cell …
Reversine, a purine analog, experienced been proved that it could induce dedifferentiation of differentiated cells into multipotent progenitor cells. acid-Schiff staining assay in hepatogenic differentiated … Manifestation of pluripotent guns and epigenetic guns To further characterize the pluripotency of reversine-pretreated cells, manifestation of specific guns 468-28-0 supplier (April4, Sox2 and Nanog) of pluripotent cells were analyzed by using RT-PCR, western blotting and immunofluorescence. In addition, we also desired to determine which gene caused the differential strength. The outcomes indicated that reversine elevated the reflection of March4 significantly, but Sox2 and Nanog had been not really discovered (Fig. ?(Fig.5,5, A and B), which indicated the account activation of Oct4 performs a major function in order of 468-28-0 supplier cell pluripotency. Remarkably, upon reversine removal after 8 times, reversine-treated fibroblasts steadily came back to primary phenotype and the ...
While investigating a rare genetic disorder, researchers at the University of California, San Diego School of Medicine have discovered that a ubiquitous signalling molecule is crucial to cellular reprogramming, a finding with significant implications for stem cell-based regenerative medicine, wound repair therapies and potential cancer treatments.
crispgg: I expect ANAN to take advantage of this situation. All those with ACCA need is the ability to set up there own firms in Nigeria. With ANAN membership, they can do that. Imagine the credibility that they will gain when they have a huge number of ACCA members joining them. In a few years, they will be at par with ICAN ...
Synthesis gas, a mixture of hydrogen and carbon monoxide, can be produced by oxidatively-reforming a hydrocarbon-containing stream which passes on one s...
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Researchers have modeled the development of neurons in some autism patients, offering what they say is a new understanding of the condition.
We have also identified different cellular compartment in these neural tumours caused by dedifferentiation. We are designing genetic tools to investigate, through lineage analyses, the cellular hierarchy governing tumour growth. We are using this new genetically tractable model to investigate the basic molecular principles governing the population of cancer stem cells. Our aim is to identify the conserved molecular pathways that need to be targeted for their elimination in order to cure cancer ...
At REPROCELL, you can gain access to the most rapid integration-free reprogramming method on the market: StemRNA™ 3rd Gen reprogramming technology.
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Lujan E, Chanda S, Ahlenius H, Südhof TC, Wernig M. Direct conversion of mouse fibroblasts to self-renewing, tripotent neural precursor cells ...
Serpine is a medicine available in a number of countries worldwide. A list of US medications equivalent to Serpine is available on the Drugs.com website.
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This process is called dedifferentiation. Dedifferentiation is the ability of plants to regain and develop into specific cell ... "History of Cell Biology". Bitesize Bio. 2007-11-05. Retrieved 2019-06-06. "Plant Cells, Chloroplasts, Cell Walls , Learn ... Plant callus is a group of parenchyma cells which can grow when needed. Parenchyma cells make up the majority of the ground ... the unharmed living cells become stimulated to divide underneath. As the living cells become divided, callus is formed and ...
Dedifferentiation also occurs in plants. Cells in cell culture can lose properties they originally had, such as protein ... fat cells, and types of bone cells Epithelial stem cells (progenitor cells) that give rise to the various types of skin cells ... Such cells, called somatic cells, make up most of the human body, such as skin and muscle cells. Cells differentiate to ... Hematopoietic stem cells (adult stem cells) from the bone marrow that give rise to red blood cells, white blood cells, and ...
This involves dedifferentiation to a pluripotent state; an example is the conversion of mouse somatic cells to an ... any cell other than a gamete, germ cell, or undifferentiated stem cell. Somatic cells are theoretically distinct from cells of ... distinct from somatic cells. The cells of the germ line are called germ cells. 2. The lineage of germ cells, spanning many ... germ cell Any biological cell that gives rise to the gametes of an organism that reproduces sexually. Germ cells are the ...
"HB-EGF is necessary and sufficient for Müller glia dedifferentiation and retina regeneration". Dev Cell. 22: 334-47. doi ... regulates differentiation and cell fate determination maintains Muller glial quiescence N-cadherin mediates cell-cell ... The signal goes first to the bipolar and horizontal cells (yellow layer), then to the amacrine cells and ganglion cells (purple ... "Nature Cell Biology. 12: 1101-1107. doi:10.1038/ncb2115. PMC 2972404.. *^ Wan, J; Ramachandran, R; Goldman, D (2012). ...
... used for stem cell dedifferentiation. It also has the potential to induce selectively cell death of cancer cells. Reversine is ... Chen, Shuibing; Zhang, Qisheng; Wu, Xu; Schultz, Peter G.; Ding, Sheng (2004). "Dedifferentiation of Lineage-Committed Cells by ... "The synthetic purine reversine selectively induces cell death of cancer cells". Journal of Cellular Biochemistry. 113 (10): ... The Journal of Cell Biology. 190 (1): 73-87. doi:10.1083/jcb.201001036. ISSN 1540-8140. PMC 2911657. PMID 20624901. Henry, ...
... cell shape, and the control of cell growth and differentiation. She asserted that the basis of many scientific ideas originate ... Hay, Elizabeth Dexter (1962). "Cytological studies of dedifferentiation and differentiation in regenerating amphibian limbs". ... the regeneration of the limb is achieved when differentiated cells begin to dedifferentiate and become stem cells. In 1957, Don ... "Epithelia suspended in collagen gels lose polarity and express characteristics of migrating mesenchymal cells". Journal of Cell ...
"Oncostatin M is a major mediator of cardiomyocyte dedifferentiation and remodeling". Cell Stem Cell. 9 (5): 420-32. doi:10.1016 ... OSMR is widely expressed across non-haematopoietic, hepatocytes, mesothelial cells, glial cells and epithelial cell types ... Intracellular cell signalling occurs as a consequence of extracellular binding of the ligand OSM to OSMR complexes, formed from ... OSM and OSMRβ are co-expressed and lead to STAT 3 activation malignant human ovarian epithelial cells. The OSMR β promoter gene ...
... "enod40 induces dedifferentiation and division of root cortical cells in legumes". Proc Natl Acad Sci U S A. 94 (16): 8901-8906 ... for elicitation of a cell-specific growth response in the alfalfa root cortex". Mol Cell Biol. 21 (1): 354-366. doi:10.1128/MCB ... Guzzo F, Portaluppi P, Grisi R, Barone S, Zampieri S, Franssen H, Levi M (2005). "Reduction of cell size induced by enod40 in ... Plant Cell. 11 (10): 1953-1966. doi:10.1105/tpc.11.10.1953. PMC 144109. PMID 10521525. Kouchi H, Takane K, So RB, Ladha JK, ...
In Physcomitrella patens the PcG protein FIE is specifically expressed in stem cells such as the unfertilized egg cell. Soon ... Consequently, loss of PcG causes de-differentiation and promotes embryonic development. Polycomb-group proteins also intervene ... Cell. 128 (4): 735-45. doi:10.1016/j.cell.2007.02.009. PMID 17320510. S2CID 6492075. Pirrotta V, Li HB (2012). "A view of ... Cell. 171 (1): 34-57. doi:10.1016/j.cell.2017.08.002. PMID 28938122. S2CID 43165761. Di Croce L, Helin K (2013). " ...
"Inducing cellular dedifferentiation: A potential method for enhancing endogenous regeneration in mammals". Seminars in Cell & ... Like stem cells in SGZ, progenitor cells in SVZ also differentiate into neurons and form an intermediate cell called a ... In the dentate gyrus, putative stem cells, called type 1 cells, proliferate into type 2 and type 3 cells, which are transiently ... Progenitor cells in the dentate gyrus of the hippocampus migrate to the nearby location and differentiate into granule cells. ...
"Intestinal tumorigenesis initiated by dedifferentiation and acquisition of stem-cell-like properties". Cell. 152 (1-2): 25-38. ... In collaboration with Hans Clevers's laboratory, Owen's group showed that Lgr5-positive cells are an efficient cell of ... Cell Stem Cell. 12 (6): 761-73. doi:10.1016/j.stem.2013.04.006. PMC 3690525. PMID 23665120. Sansom, OJ; Stark, LA; Dunlop, MG; ... "Crypt stem cells as the cells-of-origin of intestinal cancer". Nature. 457 (7229): 608-11. Bibcode:2009Natur.457..608B. doi: ...
... including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. ... IL-4 has also been shown to drive mitogenesis, dedifferentiation, and metastasis in rhabdomyosarcoma. IL-4, along with other ... The interleukin 4 (IL4, IL-4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon ... Renal cells and glioblastoma modify 10,000-13,000 receptors per cell depending on tumor type. IL-4 can primitively motivate ...
Cell. Biol. 12 (8): 3653-62. doi:10.1128/MCB.12.8.3653. PMC 364632. PMID 1321344. Shibata K, Takeda K, Tomita Y, Tagami H, ... Abe T, Sato M, Tamai M (1998). "Dedifferentiation of the retinal pigment epithelium compared to the proliferative membranes of ... Sarangarajan R, Boissy RE (2001). "Tyrp1 and oculocutaneous albinism type 3". Pigment Cell Res. 14 (6): 437-44. doi:10.1034/j. ... Tyrp1 is also involved in maintenance of melanosome structure and affects melanocyte proliferation and melanocyte cell death. ...
Particularly, ROCK inhibitor is used for cell culture practice, in part to limit cellular death and limited dedifferentiation, ... and non-small cell lung cancer (A549) cells independent of cell death induction. Fasudil, not currently approved by the U.S. ... On a cellular level, ROCK has multiple functions, including regulation of smooth muscle cell contraction, cell migration, and ... "Minimal Sustainability of Dedifferentiation by ROCK Inhibitor on Rat Nucleus Pulposus Cells In Vitro". Spine Surgery and ...
Sun T, Han X (2019). "Death versus dedifferentiation: The molecular bases of beta cell mass reduction in type 2 diabetes". ... Fatty acids in the beta cells activate FOXO1, resulting in apoptosis of the beta cells. FOXO1 activation plays a role in cell ... It suppresses survival of tumor cells by inducing apoptosis in prostate cancer cells and glioma cells by upregulating the ... In pancreatic beta cells FOXO1 mediates glucagon-like peptide-1 effects on pancreatic beta-cell mass. When the level of blood ...
It has been shown in zebrafish that Müller glia undergo dedifferentiation into multipotent progenitor cells. The progenitor ... as all glial cells do. They are the most common type of glial cell found in the retina. While their cell bodies are located in ... Damage to retinal cells results in Müller cells to undergo gliosis. The result of the response varies depending on the damage ... Aquaporin-4 in Müller cell in rat, transfer water to vitreous body. It was reported that Müller cells can be damaged by niacin ...
... dedifferentiation or self-renewal of ES cells". Nature Genetics. 24 (4): 372-376. doi:10.1038/74199. PMID 10742100. S2CID ... Also, Rex1−/Oct3/4+ cells differentiate into cells of primitive ectoderm, the somatic cell lineage. Studies have shown that ... Thus, the male germ cells undergoing meiosis are the specific cells in the testicles that express Rex1. It has not been ... During embryogenesis, the inner cell mass (ICM) is separated from the trophoblast. The stem cells derived from the ICM and ...
2,4-D is used in plant cell cultures as a dedifferentiation (callus induction) hormone. It is classified as an auxin plant ... Endreb, Rudolf (1994). Plant Cell Biotechnology. Germany: Springer. pp. 17, 18. ISBN 978-0-387-56947-5. NIOSH. Updated June ... 4-D has been used in laboratories for plant research as a supplement in plant cell culture media such as MS medium since at ...
by inducing a shift in the developmental fate of cells in the liver and converting them into 'pancreatic beta-cell-like' cells ... Despite the massive dedifferentiation - lineage tracing approach indeed demonstrates that transdifferentiation originates in ... In this approach, transcription factors from progenitor cells of the target cell type are transfected into a somatic cell to ... Patel, M.; Yang, S. (2010). "Advances in Reprogramming Somatic Cells to Induced Pluripotent Stem Cells". Stem Cell Reviews and ...
Instead dedifferentiation is observed [1-4]. These cell biological alterations arise after isolation of cells and proceed ... Cell Biol Int 34(11):1075-83. 5. Minuth WW and Denk L 2011 Advanced culture experiments with adherent cells. From single cells ... Due to these reasons a Minusheet tissue carrier with adherent cells is used only for the short period of cell seeding in a 24 ... Minusheet perfusion culture system is used for advanced cell culture experiments in combination with adherent cells and to ...
Loss of this function may lead to dedifferentiation of cells and/or unregulated cell growth and replication. Another major ... Development of cancerous cells and malignancy may be related to several of these functions. One major function includes cell- ... This protein serves numerous functions in cell to cell interactions, as well as intracellular signaling. ... in which the one unmutated copy of the CDH1 gene undergoes mutation or inactivation in some cells during the lifetime of the ...
... dedifferentiation or self-renewal of ES cells". Nature Genetics. 24 (4): 372-6. doi:10.1038/74199. PMID 10742100. S2CID ... embryonic stem cell lines (which are cell lines derived from the inner cell mass), and induced pluripotent stem cells. While ... "Oct4 expression is not required for mouse somatic stem cell self-renewal". Cell Stem Cell. 1 (4): 403-15. doi:10.1016/j.stem. ... "Oct-4 expression in adult human differentiated cells challenges its role as a pure stem cell marker". Stem Cells. 25 (7): 1675- ...
... liver cells etc. In sharp contrast to the cycle of artificial de-differentiation of somatic cells to stem cells and then the ... and re-acquire the proliferation potential of young cells. The method of PCL thus rejuvenates old cells without de- ... oocyte and to withdraw this treated cell before its de-differentiation and first cell division occurs. Thus, the progressive ... "Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells". Nat. Biotechnol. 27 (11): 1033-7 ...
... the use of adult somatic stem cells and the dedifferentiation and/or transdifferentiation of cells, and more than one mode can ... Second, the blastemal cells will undergo cell proliferation, patterning, cell differentiation and tissue growth using similar ... Macrophages are a type of repairing cell that devour dead cells and pathogens, and trigger other immune cells to respond to ... "a mound of stem cells from which regeneration begins". Dedifferentiation of cells means that they lose their tissue-specific ...
Sun T, Han X (2019). "Death versus dedifferentiation: The molecular bases of beta cell mass reduction in type 2 diabetes". ... a type 2 diabetic will have lost about half of their beta cells.[52] Fatty acids in the beta cells activate FOXO1, resulting in ... Type 2 diabetes is due to insufficient insulin production from beta cells in the setting of insulin resistance.[13] Insulin ... In the early stages of insulin resistance, the mass of beta cells expands, increasing the output of insulin to compensate for ...
... tumor cell dedifferentiation is interpreted as a reversion to phylogenetically earlier capabilities. For example, cancer cells ... or Tumour-initiating Cells. Unlike in the case of normal cells, state switching in cancer cells is widely believed to arise due ... iPS cells), tumor heterogeneity and emergence of cancer stem cells from non-stem cancer cells. Implicit in the MRK model, the ... In contrast to normal cells, a striking characteristic of cancer cells is the remarkable degree of phenotypic plasticity they ...
This latter damage type is generally considered a primary cause of cellular dedifferentiation and transdifferentiation, which ... and drift of cell and tissue-specific chromatin states. ...
Schwann cells respond to loss of axons by extrusion of their myelin sheaths, downregulation of myelin genes, dedifferentiation ... Axons have been observed to regenerate in close association to these cells. Schwann cells upregulate the production of cell ... During their proliferation phase, Schwann cells begin to form a line of cells called Bands of Bungner within the basal laminar ... 2005) observed that non-myelinated or myelinated Schwann cells in contact with an injured axon enter cell cycle thus leading to ...
In epimorphosis stem cells-either from a reserve pool or those produced by dedifferentiation-form a blastema and generate new ... vibratile cells (for coelomic fluid movement and clotting), and crystal cells (potential osmoregulatory cells in sea cucumbers ... as well as pigment cells, mechanoreceptor cells (which detect motion on the animal's surface), and sometimes gland cells which ... The coelomic fluid contains the coelomocytes, or immune cells. There are several types of immune cells, which vary among ...
Cells can become anaplastic in two ways: neoplastic tumor cells can dedifferentiate to become anaplasias (the dedifferentiation ... Anaplastic cells have lost total control of their normal functions and many have deteriorated cell structures. Anaplastic cells ... Anaplasia or dedifferentiation: loss of differentiation of cells and of their orientation to one another and blood vessels, a ... Necrotic cells send the wrong chemical signals which prevent phagocytes from disposing of the dead cells, leading to a buildup ...
"The expression of different superoxide dismutase forms is cell-type dependent in olive (Olea europaea L.) leaves". Plant & Cell ... possibly through de-differentiation of myofibroblasts back to fibroblasts.[51][further explanation needed] ... The cytosols of virtually all eukaryotic cells contain an SOD enzyme with copper and zinc (Cu-Zn-SOD). For example, Cu-Zn-SOD ... Human white blood cells use enzymes such as NADPH oxidase to generate superoxide and other reactive oxygen species to kill ...
For regeneration the salamander tissues form a blastema by de-differentiation of mesenchymal cells, and the blastema functions ... cancer cells. To compensate for the loss of telomerase in stem cells we would introduce new stem cells every decade or so. In ... Fresh cell therapy is mainly the use of live animal embryo organs cells which are injected into the patient with the purpose of ... Stem cell regenerative medicine uses three different strategies: Implantation of stem cells from culture into an existing ...
1997). "CD97: a dedifferentiation marker in human thyroid carcinomas". Cancer Res. 57 (9): 1798-806. PMID 9135025. Cite uses ... 1998). "Phenotypic characterization of human skin mast cells by combined staining with toluidine blue and CD antibodies". J. ... 2003). "The epidermal growth factor-like domains of the human EMR2 receptor mediate cell attachment through chondroitin sulfate ... "Expression and Regulation of CD97 in Colorectal Carcinoma Cell Lines and Tumor Tissues". Am. J. Pathol. 161 (5): 1657-67. PMC ...
Embryonic cell lines and autologous embryonic stem cells generated through somatic cell nuclear transfer or dedifferentiation ... Stem cell division and differentiation A: stem cell; B: progenitor cell; C: differentiated cell; 1: symmetric stem cell ... mesenchymal stem cell, adipose-derived stem cell, endothelial stem cell, dental pulp stem cell, etc.).[37][38] Muse cells ( ... from adult cells. These are not adult stem cells, but adult cells (e.g. epithelial cells) reprogrammed to give rise to cells ...
... the use of adult somatic stem cells and the dedifferentiation and/or transdifferentiation of cells, and more than one mode can ... Macrophages are a type of repairing cell that devour dead cells and pathogens, and trigger other immune cells to respond to ... "a mound of stem cells from which regeneration begins".[21] Dedifferentiation of cells means that they lose their tissue- ... First, adult cells de-differentiate into progenitor cells which will replace the tissues they are derived from.[56][57] Second ...
... a maturation arrest of progenitor cells, or as a result of dedifferentiation of mature cells.[91] ... Cancer stem cells[edit]. Main article: Cancer stem cell. The first malignant cell, that gives rise to the tumor, is often ... When a cancer cell divides, both daughter cells inherit the genetic and epigenetic abnormalities of the parent cell, and may ... Thus, a cell that acquires a mutation that increases its fitness will generate more daughter cells than competitor cells that ...
... exocrine tissue by coexpressed cell surface glycoprotein CD133 have a transcriptome consistent with exocrine dedifferentiation ... "Loss of Fbw7 reprograms adult pancreatic ductal cells into α, δ, and β cells". Cell Stem Cell. 15 (2): 139-53. doi:10.1016/j. ... "Reprogramming of human pancreatic exocrine cells to β-like cells". Cell Death and Differentiation. 22 (7): 1117-30. doi:10.1038 ... exocrine cells have been reprogrammed into cells with an islet cell-like phenotype following direct expression of NGN3 or ...
Further analyses pointed to a possible dedifferentiation process and suggested that most of the transcriptomic dysregulations ... Juvenile Granulosa Cell Tumors[edit]. Two recent studies show that the enzyme AKT1 is involved in juvenile granulosa cell ... Adult Granulosa Cell Tumors[edit]. Using next generation DNA sequencing, it was discovered that 97% of adult granulosa cell ... Granulosa cell tumours are tumours that arise from granulosa cells. They are esterogen secreting tumors and present as large, ...
Sugimoto K, Gordon SP, Meyerowitz EM (April 2011). "Regeneration in plants and animals: dedifferentiation, transdifferentiation ... Cell potency is a cell's ability to differentiate into other cell types.[1][2][3] The more cell types a cell can differentiate ... In cell biology, a unipotent cell is the concept that one stem cell has the capacity to differentiate into only one cell type. ... can give rise to various blood cells such as B and T cells, however, not to a different blood cell type like a red blood cell.[ ...
desember 2012). «Embryonic stem cell and induced pluripotent stem cell: an epigenetic perspective». Cell Research 23 (1): 49-69 ... Sugimoto K, Gordon SP, Meyerowitz EM (April 2011). «Regeneration in plants and animals: dedifferentiation, transdifferentiation ... Baker, Monya (6. desember 2007). «Adult cells reprogrammed to pluripotency, without tumors». Nature Reports Stem Cells.. ... Cell-Off: Induced Pluripotent Stem Cells Fall Short of Potential Found in Embryonic Version». Scientific American. Henta 25. ...
... dedifferentiation and rapid cell proliferation, have been pointed out [13].. Recently, tumor growth has been studied using ... for B cell lesions) or T cell receptor gene (for T cell lesions). The demonstration of clonality is now considered to be ... These cells are presumed to be clonal - that is, they are derived from the same cell,[8] and all carry the same genetic or ... A mutant or epigenetically altered stem cell may replace the other nearby stem cells by natural selection. Thus, a patch of ...
The surviving epithelial cells, however, undergo migration, dedifferentiation, proliferation, and redifferentiation to ... It has been shown that bone marrow-derived cells could be the source of progenitor cells of multiple cell lineages, and a 2004 ... In addition to the surviving tubular epithelial cells and kidney stem cells, the bone marrow stem cells have also been shown to ... Smith, Dana G. (28 April 2016). ""Scientists turn skin cells into heart cells and brain cells using drugs: Studies represent ...
cell-cell signaling. • G-protein coupled receptor signaling pathway. • cell surface receptor signaling pathway. • movement of ... a dedifferentiation marker in human thyroid carcinomas". Cancer Research. 57 (9): 1798-806. PMID 9135025.. ... immune cells, epithelial cells, muscle cells as well as their malignant counterparts.[12][13][14][15][16][17] In the case of ... Aust G, Wandel E, Boltze C, Sittig D, Schütz A, Horn LC, Wobus M (Apr 2006). "Diversity of CD97 in smooth muscle cells". Cell ...
"Melanomas resist T-cell therapy through inflammation-induced reversible dedifferentiation.", Nature Oct 18;490(7420):412-6., ... "Immune cell-poor melanomas benefit from PD-1 blockade after targeted type I IFN activation.", Cancer Discov. Jun;4(6):674-87, ...
... however callus and somatic embryos are derived from mesophyll cells that undergo dedifferentiation. Plant hormones are used to ... Plant callus (plural calluses or calli) is a growing mass of unorganized plant parenchyma cells. In living plants, callus cells ... Regeneration of a whole plant that has some genetically transformed cells and some untransformed cells yields a chimera. In ... In Jatropha curcas callus cells, small organized callus cells became disorganized and varied in size after browning occurred. ...
... rather than dedifferentiation of specialized cells, account for undifferentiated tumors. Anaplastic cells display marked ... It implies dedifferentiation, or loss of structural and functional differentiation of normal cells. It is now known, however, ... Also, anaplastic cells usually fail to develop recognizable patterns of orientation to one another (i.e., they lose normal ... The nuclear-cytoplasmic ratio may approach 1:1 instead of the normal 1:4 or 1:6. Giant cells that are considerably larger than ...
Cobaleda, C; Jochum, W; Busslinger, M (27 September 2007). "Conversion of mature B cells into T cells by dedifferentiation to ... The lymphoid lineage is composed of T-cells, B-cells and natural killer cells. This is lymphopoiesis. Cells of the myeloid ... All blood cells are divided into three lineages. Red blood cells, also called erythrocytes, are the oxygen-carrying cells. ... Each successive change moves the cell closer to the final cell type and further limits its potential to become a different cell ...
"Up-regulation of neural stem cell markers suggests the occurrence of dedifferentiation in regenerating spinal cord". ... Stem cell markers are genes and their protein products used by scientists to isolate and identify stem cells. Stem cells can ... 2005). "Somatic stem cell marker prominin-1/CD133 is expressed in embryonic stem cell-derived progenitors". Stem Cells. 23 (6 ... Hirao A, Arai F, Suda T (December 2004). "Regulation of cell cycle in hematopoietic stem cells by the niche". Cell Cycle. 3 (12 ...
"In CK2 inactivated cells the cyclin dependent kinase inhibitor Sic1 is involved in cell-cycle arrest before the onset of S ... Low p27Kip1 expression can lead to tumor de-differentiation, increased penetration through the gastric wall, lymph node ... Cell. 79 (2): 233-44. doi:10.1016/0092-8674(94)90193-7. PMID 7954792. S2CID 34939988. Morgan DO (1997). The Cell Cycle: ... In the G1 phase of the cell cycle, Sic1 binds tightly to the Cdc28-Clb complex and inhibits it. Low Cdc28-Clb activity leads to ...
However, benign desmoplasias do not exhibit dedifferentiation of tumor cells. A desmoplastic response is characterized by ... or parenchymal cells of epithelia, are highly organized, polar cells. These cells are separated from stromal cells by a ... Normal tissues consist of parenchymal cells and stromal cells. The parenchymal cells are the functional units of an organ, ... doi:10.1016/j.cell.2011.02.013. PMID 21376230. Alberts, B; Johnson, A; Lewis, J (2008). Molecular Biology of the Cell (5th ed ...
Cell Metab. 2018 Jun 5;27(6):1294-1308.e7. doi: 10.1016/j.cmet.2018.04.013. Epub 2018 May 10. ... Eed; Polycomb; cell identity; chromatin; complex diseases; de-differentiation; diabetes; epigenetic; type 2 diabetes; β cells ... The Polycomb-Dependent Epigenome Controls β Cell Dysfunction, Dedifferentiation, and Diabetes.. Lu TT1, Heyne S1, Dror E1, ... β cell-specific Polycomb (Eed/PRC2) loss of function in mice triggers diabetes-mimicking transcriptional signatures and highly ...
Mitochondria and cancer: a growing role in apoptosis, cancer cell metabolism and dedifferentiation.. Scatena R1. ... Moreover, studying the role of mitochondria in cancer cell dedifferentiation/differentiation processes may allow further ... At the beginning of the twentieth century, Otto Warburg demonstrated that cancer cells have a peculiar metabolism. These cells ... Over time, however, many studies have shown that mitochondria play a fundamental role in cell death by apoptosis or necrosis. ...
Dedifferentiation of neoplastic cells in medullary thyroid carcinoma: Report of a case. ... These cytological changes might represent dedifferentiation of the neoplastic cells or the anaplastic transformation of MTC. ... Nishiyama RH, Dunn EL, Thompson NW (1972) Anaplastic spindle-cell and giant-cell tumors of the thyroid gland. Cancer 30:113-127 ... Roediger WEW (1973) A comparative study of the normal human neonatal and the canine thyroid C cell. J Anat 115:255-276PubMed ...
Until recently, the decline of insulin production had been ascribed to β-cell death. But recent research has shown that β-cells ... When β-cells fail: lessons from dedifferentiation Diabetes Obes Metab. 2016 Sep;18 Suppl 1(Suppl 1):117-22. doi: 10.1111/dom. ... But recent research has shown that β-cells do not die in diabetes, but undergo a silencing process, termed "dedifferentiation ... This capability is the first casualty in the progress of β-cell failure. β-Cells lose the ability to select the right fuel for ...
... Jin-Feng Li, ... "Aging and insulin signaling differentially control normal and tumorous germline stem cells," Aging Cell, vol. 14, no. 1, pp. 25 ... "Mesenchymal stem cells derived from human iPS cells via mesoderm and neuroepithelium have different features and therapeutic ... Michał Pikuła, Paulina Langa, Paulina Kosikowska, and Piotr Trzonkowski, "Stem cells and growth factors in wound healing," ...
H. Li, X. Fu, L. Zhang, T. Sun, and J. Wang, "In vivo dedifferentiation of human epidermal cells," Cell Biology International, ... "Dedifferentiation derived cells exhibit phenotypic and functional characteristics of epidermal stem cells," Journal of Cellular ... HGF Accelerates Wound Healing by Promoting the Dedifferentiation of Epidermal Cells through -Integrin/ILK Pathway. Jin-Feng Li, ... P. H. Jones, S. Harper, and F. M. Watt, "Stem cell patterning and fate in human epidermis," Cell, vol. 80, no. 1, pp. 83-93, ...
... the potential to develop into any cell type within an organism. This astonishing plasticity may contribute to a high ... Plant somatic cells are generally acknowledged to retain totipotency, ... PRC2 represses dedifferentiation of mature somatic cells in Arabidopsis Nat Plants. 2015 Jun 29;1:15089. doi: 10.1038/nplants. ... Plant somatic cells are generally acknowledged to retain totipotency, the potential to develop into any cell type within an ...
In addition, the ability of transit-amplifying cells to become stem cells may be shared by other stem cell systems [reviewed in ... G) Day 6; a spermatocyte cyst (outlined, large arrowhead) and a few Vasa-negative cells (somatic stem cells or cyst cells, ... Regeneration of Male Germline Stem Cells by Spermatogonial Dedifferentiation in Vivo Message Subject. (Your Name) has forwarded ... The ability to study induced dedifferentiation in an intact stem cell niche should greatly aid our understanding of the ...
What is the source of your epithelial cell? The morphology cannot certify that a cell is undergoing de-differentiation. You ... What is the source of your epithelial cell? The morphology cannot certify that a cell is undergoing de-differentiation. You ... if you find and prove the dedifferentiation of special cells, you may deserve a big paper!! QUOTE (Fetus @ Mar 10 2006, 08:28 ... What are the morphologic features of cells undergoing dedifferentiation - (Feb/24/2006 ). Hello All,. Could someone tell me ...
Abstract 4376: Acquired cancer stem cell like features through Oct4 mediated dedifferentiation. Suresh M.K Kumar, Shujing Liu, ... Abstract 4376: Acquired cancer stem cell like features through Oct4 mediated dedifferentiation ... of Oct4 gene or transmembrane delivery of Oct4 protein promoted dedifferentiation of melanoma cells to CSC-like cells. The ... Abstract 4376: Acquired cancer stem cell like features through Oct4 mediated dedifferentiation ...
Conclusions-De-differentiation of fibroblasts to intermediate CD34+ progenitors gives rise to endothelial cells and ... SOX17 Regulates Conversion of Human Fibroblasts into Endothelial Cells and Erythroblasts via De-Differentiation into CD34+ ... SOX17 Regulates Conversion of Human Fibroblasts into Endothelial Cells and Erythroblasts via De-Differentiation into CD34+ ... SOX17 Regulates Conversion of Human Fibroblasts into Endothelial Cells and Erythroblasts via De-Differentiation into CD34+ ...
Lastly, we identified SOX9 targets in human β cells as potentially new markers of dedifferentiation in T1D. These findings ... We undertook this study to examine whether viral infection could induce human β cell dedifferentiation. Using the functional ... SOX9 was induced by the NF-κB pathway and also in a paracrine non-cell-autonomous fashion through the secretion of IFN-α. ... Recent data suggest that viral infection of human islets leads to a decrease in insulin production rather than β cell death, ...
... a process in which β-cells lose expression of markers of maturity and gain those of endocrine progenitors. Failing β-cells ... B-Cyb5r3 β-cells lose expression of Glut2, MafA, and Pdx1 expression despite a compensatory increase in FoxO1 expression. Our ... In vivo, mice with β-cell-specific ablation of Cyb5r3 (B-Cyb5r3) display impaired glucose tolerance with decreased insulin ... We found a striking enrichment in the expression of aldehyde dehydrogenase 1 isoform A3 (Aldh1a3) as β-cells become ...
Lastly, we identified SOX9 targets in human β cells as potentially new markers of dedifferentiation in T1D. These findings ... We undertook this study to examine whether viral infection could induce human β cell dedifferentiation. Using the functional ... EndoC-βH1 cells were treated with proinflammatory cytokines (TNF-α, IL-1β, and IFN-γ). (. A. ) SOX9. , HES1. , MYC. , MAFA. , ... EndoC-βH1 cells were transfected with control nontarget siRNA (siCTRL) or siRNA targeting RELA (siRELA). They were next treated ...
NPM-ALK induces reprogramming of mature TCR-stimulated T cells resulting in de-differentiation and malignant transformation. ... NPM-ALK induces reprogramming of mature TCR-stimulated T cells resulting in de-differentiation and malignant transformation ... NPM-ALK induces reprogramming of mature TCR-stimulated T cells resulting in de-differentiation and malignant transformation ... NPM-ALK induces reprogramming of mature TCR-stimulated T cells resulting in de-differentiation and malignant transformation ...
Novel Genes Mediating the De-Differentiation of Human Corneal Epithelial Cells E. Y. Chuang; F. Bian; S. C. Pflugfelder; D. Q. ... De-differentiation occurred when differentiated cells sub-cultured from SHEM to CnT-20 medium. The higher expression of TCF4, ... suggesting that this defined medium preserves more stem cell like characteristics than cells cultured in SHEM. When cells ... Novel Genes Mediating the De-Differentiation of Human Corneal Epithelial Cells You will receive an email whenever this article ...
Cells undergo reprogramming/de-differentiation through nuclear and cytoplasmic remodelling. Autophagy is a physiological stress ... Human multipotent stromal cells/mesenchymal stem cells (MSCs) are cells of mesenchymal origin, which are cultured as adherent ... MSCs can also be cultured as 3D cell aggregates or spheroids. Variations in spheroid size should result in variations in ... of three dimensional culture mechanisms to induce de-differentiation and reprogramming in human multipotent stromal cells ...
Spindle shaped cells and round cells at the response site are positive. (D) HHF-35. The spindle shaped cells and round cells ... Role of endothelial cell denudation and smooth muscle cell dedifferentiation in neointimal formation of human vein grafts after ... Role of endothelial cell denudation and smooth muscle cell dedifferentiation in neointimal formation of human vein grafts after ... Both spindle shaped cells and round cells in the neointima are positive. (C) HHF-35. The cells in the neointima do not stain. ( ...
Dedifferentiation of Tobacco Cells Is Associated with Ribosomal RNA Gene Hypomethylation, Increased Transcription, and ... Dedifferentiation of Tobacco Cells Is Associated with Ribosomal RNA Gene Hypomethylation, Increased Transcription, and ... Dedifferentiation of Tobacco Cells Is Associated with Ribosomal RNA Gene Hypomethylation, Increased Transcription, and ... Dedifferentiation of Tobacco Cells Is Associated with Ribosomal RNA Gene Hypomethylation, Increased Transcription, and ...
Dedifferentiation, redifferentiation and bundle formation of smooth muscle cells in tissue culture: the influence of cell ... Dedifferentiation, redifferentiation and bundle formation of smooth muscle cells in tissue culture: the influence of cell ... Dedifferentiation, redifferentiation and bundle formation of smooth muscle cells in tissue culture: the influence of cell ... Dedifferentiation, redifferentiation and bundle formation of smooth muscle cells in tissue culture: the influence of cell ...
... besides cell death, as a contributing factor to the loss of functional beta-cell mass in diabetes. beta-cell dedifferentiation ... Finally, it proposes a novel hypothesis of beta-cell dedifferentiation as a potential adaptive mechanism to escape cell death ... While the major role of glucotoxicity in beta-cell dedifferentiation is well established, the precise mechanisms involved are ... This review highlights the identified molecular mechanisms implicated in beta-cell dedifferentiation including oxidative stress ...
De-differentiation of papillary thyroid carcinoma into squamous cell carcinoma. A case of coexistence within an excised neck ... De-differentiation of papillary thyroid carcinoma into squamous cell carcinoma. A case of coexistence within an excised neck ...
... or chondrogenic-differentiated cells proceeds via dedifferentiation and have a correlation with cell cycle arresting and ... Transdifferentiation of mesenchymal stem cells-derived adipogenic-differentiated cells into osteogenic- ... The process of dedifferentiation was also confirmed by single cell clonal analysis. Finally the dedifferentiated cells were ... This leads to the questions that whether transdifferentiation occurs via direct cell-to-cell conversion or dedifferentiation to ...
Cells were seeded at a density of 10^5 cells/cm2 in 2D culture. Different parameters related to cell death and proliferation ... JHH2 and JHH4 cell lines obtained from the Japanese Collection of Research Bioresources Cell Bank (Tokyo, Japan). Cells were ... Effect of cell dedifferentiation and genetic p53 profile in the expression of Bcl-2 family members in tyrosine kinase inhibitor ... Conclusions: The dedifferentiation of cancer cells and mutated p53 reduce the upregulaupregulation of Bim and Bid induced by ...
... that cells are at or what stage of DEDIFFERENTIATION (going backward) that cancer cells are at. What if, after transformation ... Stem cell videos. *Stem cells in your languageIve been working on an outreach program I call the Knoepfler Lab Stem Cell ... 3 thoughts on "Groundbreaking Salk study shows de-differentiation of mature neurons & astrocytes into glioma forming cells" * ... Interestingly, the glioma cells exhibited many hallmarks of stem cells.. An important open question is whether the neurons are ...
B cell acute lymphoblastic leukemia relapse following CD19 CAR immune-pressure via de-differentiation and lineage switch. ... Our data demonstrates plasticity of leukemic cells under antigen-specific immune pressure, with de-differentiation and lineage ... Later relapses had loss of all the B cell surface markers, along with down regulation of B cell transcripts (such as EBF1 and ... CD19 is a surface marker restricted to B cells and highly expressed on B cell ALL. CD19 has been targeted through genetically ...
The development process from single cell of EPMSC to embryo-like cells and iPSCs. (a) one cell, (b) two cells, (c) 8 cells, (d ... To date, germ-like cells can be induced from fetal stem cells [17], bone marrow stem cells [18, 19], pancreatic stem cells [20 ... One cell, two cells, 4 cells, blastocyst and hatching blastocyst presented at the same time (Fig. 3). Approximately 50% cells ... After that, a lot cells with broken cell membranes with hatched cell mass could be observed (Fig. 4). The embryo like cells ...
The pluripotent cells may be oocytes, blastomeres, inner cell mass cells, embryonic stem cells, embryonic germ cells, embryos ... consisting of one or more cells, embryoid body (embryoid) cells, morula-derived cells, teratoma (teratocarcinoma) cells, as ... the cell can be induced to re-differentiate into a different somatic cell type. A method for de-differentiating a somatic cell ... and introducing components of cytoplasm of plutipotent cells into the somatic cell and allowing the cell to de-differentiate. ...
More from: Stem Cell Dedifferentiation. TOR (focus on of rapamycin) can be an evolutionarily conserved nutrient. P April 28, ... Objective To examine the frequency and phenotype of Th17 cells in * Wig-1 is definitely a transcriptional target of the tumor ... Objective To examine the frequency and phenotype of Th17 cells in * Wig-1 is definitely a transcriptional target of the tumor ... evolutionarily conserved nutrient sensing proteins kinase that regulates development and metabolism in every eukaryotic cells. ...
Cell topography and mechanics trended resembled those of the genes expression. Integrin β1 was expressed in ADSCs and rapidly ... amount and distribution of integrin β1 may play a critical role in mediating both chondroid cell maturity and dedifferentiation ... Integrin β1 is a possible new marker and target for phenotypic maintenance in chondroid cells. ... Morphological and biomechanical properties of cells undergoing chondrogenic differentiation from human adipose-derived stem ...
  • Some of these data indicate that modulation of mitochondrial respiration may induce an arrest of cancer cell proliferation and differentiation (pseudodifferentiation) and/or or death, suggesting that iatrogenic manipulation of some mitochondrial activities may induce anticancer effects. (nih.gov)
  • Moreover, studying the role of mitochondria in cancer cell dedifferentiation/differentiation processes may allow further insight into the pathophysiology and therapy of so-called cancer stem cells. (nih.gov)
  • By conditionally manipulating Jak-STAT signaling, we find that spermatogonia that have initiated differentiation and are undergoing limited mitotic (transit-amplifying) divisions can repopulate the niche and revert to stem cell identity. (sciencemag.org)
  • The morphology cannot certify that a cell is undergoing de-differentiation. (protocol-online.org)
  • The dedifferentiated melanoma cells showed significantly decreased expression of melanocytic markers and acquired many features associated with CSCs such as multipotent differentiation capacity and expression of melanoma CSC markers such as ABCB5 and CD271. (aacrjournals.org)
  • Background -The mechanisms underlying the de-differentiation and lineage conversion of adult human fibroblasts into functional endothelial cells have not yet been fully defined. (ahajournals.org)
  • Furthermore, it is not known whether fibroblast de-differentiation recapitulates the generation of multipotent progenitors during embryonic development which give rise to endothelial and hematopoietic cell lineages. (ahajournals.org)
  • Conclusions -De-differentiation of fibroblasts to intermediate CD34 + progenitors gives rise to endothelial cells and erythroblasts in a SOX17-dependent manner. (ahajournals.org)
  • Real time PCR analysis revealed that the cells cultured in the defined media expressed a higher mRNA levels of stem cell associated genes ABCG2 and p63 and lower levels of differentiation genes K12 and SPRR2B than cells grown in SHEM media, suggesting that this defined medium preserves more stem cell like characteristics than cells cultured in SHEM. (arvojournals.org)
  • When those cells were returned to SHEM, differentiation occurred and the cells did not reach two passages. (arvojournals.org)
  • De-differentiation occurred when differentiated cells sub-cultured from SHEM to CnT-20 medium. (arvojournals.org)
  • The higher expression of TCF4, DLL-1, GAS-1, p57 and NGF genes in cells cultured in CnT-20 suggests a possible role in the mechanism for de-differentiation of human corneal epithelial cells. (arvojournals.org)
  • Cells undergo reprogramming/de-differentiation through nuclear and cytoplasmic remodelling. (bl.uk)
  • Together these results suggest that a scaled autophagy response could play a fundamental role in reprogramming/de-differentiation and tissue regeneration in human cells. (bl.uk)
  • Epigenetic changes accompanying plant cell dedifferentiation and differentiation are reported in 35S ribosomal DNA (rDNA) of tobacco ( Nicotiana tabacum ). (plantphysiol.org)
  • Like all the cells of an organism, pancreatic beta-cells originate from embryonic stem cells through a complex cellular process termed differentiation. (garvan.org.au)
  • De-differentiation of papillary thyroid carcinoma into squamous cell carcinoma. (bmj.com)
  • It is generally accepted that after differentiation bone marrow mesenchymal stem cells (MSC) become lineage restricted and unipotent in an irreversible manner. (eurekamag.com)
  • This leads to the questions that whether transdifferentiation occurs via direct cell-to-cell conversion or dedifferentiation to a progenitor cells and subsequent differentiation, and whether MSC potency decreases or increases during differentiation. (eurekamag.com)
  • 7]. The aim of the present study was the comparative analysis of the tyrosine kinase inhibitors in cell death and proliferation, and the expression of Bcl-2 family members according to the differentiation degrees and p53 genetic profile in liver cancer cells. (upo.es)
  • We propose that the genetically altered differentiated cell acquires the capacity to dedifferentiate to a more progenitor (stem cell) state, and that tumor progression probably requires a permissive microenvironment composed of cell types and molecular signals that can sustain both differentiation of tumor cells as well as maintenance of tumor stem-like cells. (ipscell.com)
  • There would appear to be overwhelming evidence that OXYGEN Tension is the PRIMARY differentiating factor, ie that which determines at what stage of development or differentiation (going forward) that cells are at or what stage of DEDIFFERENTIATION (going backward) that cancer cells are at. (ipscell.com)
  • Our data demonstrates plasticity of leukemic cells under antigen-specific immune pressure, with de-differentiation and lineage switch. (nih.gov)
  • By introduction of Oct4, Sox2, Klf4 and cMyc, human adult somatic cells can be reprogrammed into embryonic stem cell capable of pluripotent differentiation. (ijbs.com)
  • After culturing the embryo-like cells for one month, the induced pluripotency stem cells (iPSC) could be formed (proved by pluripotency gene expression, by in vitro and in vivo differentiation). (ijbs.com)
  • Overall, our data suggests that MYO1E contributes to breast tumor malignancy and regulates the differentiation and proliferation state of breast tumor cells. (oncotarget.com)
  • S-Nitroso-N-acetylcysteine induces de-differentiation of activated hepatic stellate cells and promotes antifibrotic effects in vitro. (bvsalud.org)
  • Although several linker histone variants have been studied in both animal and plant organisms, little is known about their distribution during processes that involve alterations in chromatin function, such as differentiation, dedifferentiation and hormone treatment . (bvsalud.org)
  • Each variant's ratio to total Hl in the three developmental zones of maize ( Zea mays L.) root and in callus cultures derived from them was estimated in order to define possible alterations either during plant cell differentiation or during their dedifferentiation. (bvsalud.org)
  • We propose that the alterations in linker histone variants' ratios are correlated with plant cell differentiation and dedifferentiation. (bvsalud.org)
  • Transcription factors (TFs) are able to regulate differentiation-related processes, including dedifferentiation and direct conversion, through the regulation of cell type-specific transcriptional profiles. (elsevier.com)
  • We anticipate that this functional correlation might be applicable in various cell types and might facilitate the identification of TFs with induction activity in efforts to understand differentiation. (elsevier.com)
  • In a cancer diagnosis , histology describes the type, behavior, and differentiation (grade) of tumor cells. (in.gov)
  • Genome-wide CRISPR Screens in T Helper Cells Reveal Pervasive Crosstalk between Activation and Differentiation. (sanger.ac.uk)
  • These networks provide barriers against trans-differentiation (i.e. loss of beta-cell identity). (genewhisperer.com)
  • Important studies, both in wild-type and in genetic models, have pointed out that loss of beta cell identity (i.e. 'dedifferentiation' or 'trans-differentiation') is associated with increased metabolic stress (which is commonly seen with obesity and inflammation). (genewhisperer.com)
  • The sea cucumber Myc is a homolog of mammalian Myc proteins, global transcription regulators that moderate expression of 10-15 % of the genome and play crucial roles in control of cell growth, proliferation, balance between self-renewal and differentiation, and apoptosis in various developmental contexts and during oncogenic transformation [ 11 - 14 ]. (biomedcentral.com)
  • Dedifferentiated fat cells (DFAT cells), obtained with the help of ceiling cultivation, are an interesting model for the study of the reversibility of adipogenic differentiation. (radiotec.ru)
  • Although the phenomenon of dedifferentiation was discovered about 40 years ago, the return to less differentiated state of cells, which passed their initial differentiation in the culture in vitro, has not been experimentally shown yet. (radiotec.ru)
  • In this regard, the purpose of this study was to compare the ability of dedifferentiation of primary adipocytes and adipocytes obtained as a result of in vitro differentiation of mesenchymal stromal cells (MSCs). (radiotec.ru)
  • Thus, dedifferentiation is shown to be characteristic not only for adipocytes isolated from tissue material, but also for cells obtained as a result of previous differentiation of MSCs in vitro. (radiotec.ru)
  • This approach can be used for selection of cells aimed at creating an adequate model of the reversibility of adipogenic differentiation. (radiotec.ru)
  • GAS5 is related to osteoporosis by regulating the differentiation of bone marrow stromal cells that acts as a promising treatment target for osteoporosis. (elifesciences.org)
  • During ES cell differentiation, Yap1 directly regulates apoptosis-related genes like Bcl-2 and Mcl-1 to attenuate apoptosis and promote cell survival to allow for successful cell fate changes. (elifesciences.org)
  • PDK1 serves as a critical role for intrinsically promoting T follicular helper cell differentiation and effector functions via controlling expression level of TCF1. (elifesciences.org)
  • Characterization of Drosophila female germ cell differentiation shows that nurse cells initiate Polycomb silencing by regulating Pcl and Scm levels to alter the biochemical properties of the PRC2 H3K27 methylase. (elifesciences.org)
  • Mammalian germ layer differentiation is characterized by transitions through discrete cell states, which are transcriptionally as well as functionally distinct. (elifesciences.org)
  • MLL4 (KMT2D) is a major mammalian H3K4 mono- and di-methyltransferase that is essential for enhancer activation, cell-type-specific gene expression, and cell differentiation. (elifesciences.org)
  • Inflammation-induced differentiation may be a mechanism of resistance to adoptive T-cell therapies. (aacrjournals.org)
  • 2015) and subsequently, how chromatin remodelling, within the context of a GRN affects cell type-specific patterns of gene expression to regulate developmental decisions, such as cell proliferation and differentiation(de Lucas et. (dur.ac.uk)
  • Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. (uniprot.org)
  • Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. (uniprot.org)
  • Plays also a role in preventing reversal of muscle cell differentiation. (uniprot.org)
  • Down-regulation of myogenin can reverse terminal muscle cell differentiation. (uniprot.org)
  • They proposed that these were pluripotent hematopoietic stem cells (HSCs) ( 4-6 ) that had the property, at the single-cell level, of (i) self-renewal as well as (ii) multilineage differentiation. (sciencemag.org)
  • High glucose via NOX-dependent ROS generation and AKT activity promotes adipose-derived stem cell de-differentiation. (unboundmedicine.com)
  • We also show that miR-30 family microRNAs increase in abundance during differentiation of pancreatic islet-derived mesenchymal cells into hormone-producing islet-like cell aggregates. (biomedsearch.com)
  • Cellular differentiation is the process in which a cell changes from one cell type to another. (wikipedia.org)
  • Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. (wikipedia.org)
  • Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. (wikipedia.org)
  • Although metabolic composition does get altered quite dramatically where stem cells are characterized by abundant metabolites with highly unsaturated structures whose levels decrease upon differentiation. (wikipedia.org)
  • During terminal differentiation, a precursor cell formerly capable of cell division, permanently leaves the cell cycle, dismantles the cell cycle machinery and often expresses a range of genes characteristic of the cell's final function (e.g. myosin and actin for a muscle cell). (wikipedia.org)
  • Differentiation may continue to occur after terminal differentiation if the capacity and functions of the cell undergo further changes. (wikipedia.org)
  • We are interested in understanding the role of the extracellular matrix in regulating stem cell self-renewal and differentiation, and how this regulation goes awry in cancer. (stanford.edu)
  • The role and effect of 5hmC on 5mC turnover and hence DNA demethylation, on gene expression per se and stem cell fate and differentiation is a topic of vigorous interest. (stanford.edu)
  • Here, we report for the first time that DEPTOR maintains the terminal differentiation of MM cells. (springermedizin.de)
  • Due to their release of pro-inflammatory cytokines, NK cells may potently reverse the anti-inflammatory tumor microenvironment (TME) and augment adaptive immune responses by promoting differentiation, activation, and/or recruitment of accessory immune cells to sites of malignancy. (frontiersin.org)
  • The generation of transcriptional differences between cells is the basis of most differentiation and disease. (ucl.ac.uk)
  • The data indicate that Neurog3 + enteroendocrine progenitors require active Foxo1 to prevent differentiation into Ins + cells. (nbdiabetes.org)
  • Maintained in adipogenic media, the reversine-pretreated annulus cells displayed evidence of adipogenic differentiation: accumulation of cytosolic lipid droplets and increased expression of PPAR-gamma 2, LPL, and Fabp mRNA. (bjmu.edu.cn)
  • It is concluded that reversine treatment induced annulus fibrosus cell plasticity and promoted their differentiation along mesenchymal lineages. (bjmu.edu.cn)
  • Deciphering the "communication with" and "education from" neighboring cells a stem cell and its progeny receives during the journey to a differentiated end point is the key to success in controlled re-differentiation of self-renewing cells for regenerative purposes. (clarkson.edu)
  • Method Of Effecting De-Differentiation Of A Cell. (clarkson.edu)
  • Cell differentiation refers to the normal process by which a less specialized cell goes through development and maturation in order to become more distinct in terms of form and function . (biology-online.org)
  • An example of cell differentiation is the development of a single-celled zygote into a multicellular embryo that further develops into a more complex multisystem of distinct cell types of a fetus . (biology-online.org)
  • A cell that underwent differentiation is described as differentiated . (biology-online.org)
  • An undifferentiated cell therefore would be a primitive cell or a progenitor cell that is yet to undergo cellular differentiation. (biology-online.org)
  • Her lab is currently investigating how spermatogonia reverse differentiation to become germ line stems cells again. (hopkinsmedicine.org)
  • Dr. Matunis studies the stem cells that sustain spermatogenesis in the fruit fly Drosophila melanogaster to understand how signals from neighboring cells control stem cell renewal or differentiation. (hopkinsmedicine.org)
  • She and her team are also investigating how spermatogonia reverse differentiation to become germ line stem cells again. (hopkinsmedicine.org)
  • Results -iECs expressed specific endothelial cell surface markers and also exhibited the capacity for cell proliferation and neovascularization. (ahajournals.org)
  • CONCLUSIONS The observation that luminal endothelial cell denudation, with insudation of the intima with blood elements, occurs in the very early stages suggests that these phenomena are responsible for the observed dedifferentiation of pre-existent smooth muscle cells, known to be a prerequisite for cell proliferation and the evolution of intimal thickening. (bmj.com)
  • The data together indicate that the establishment of pluripotency and cell proliferation occurring with callus induction is associated with enhanced ribosomal RNA gene expression and overall rDNA hypomethylation, but is not associated with material-enhanced relaxation of chromatin structure (decondensation) at rDNA loci. (plantphysiol.org)
  • At 7 days of culture these dedifferentiated smooth muscle cells underwent extensive proliferation. (biologists.org)
  • The process of dedifferentiation and proliferation was delayed in those smooth muscle cells which had sympathetic nerve fibres in close association. (biologists.org)
  • We found that dedifferentiated cells have a multilineage potency comparable to MSC, and also observed the associative role of proliferation genes with cell cycle arrest and progression. (eurekamag.com)
  • Different parameters related to cell death and proliferation were associated with the expression of Bcl-2 family members assessed by Western-blot analysis. (upo.es)
  • The administration of tyrosine kinase inhibitors induced cell death and reduced cell proliferation. (upo.es)
  • Underlying these changes, inhibitory cell cycle molecules were suppressed in myocyte-derived cells (MDCs), while microRNAs known to orchestrate proliferation and pluripotency increased dramatically. (pubmedcentralcanada.ca)
  • Dedifferentiation of myocytes facilitates proliferation and confers a degree of stemness, including the expression of c-kit and the capacity for multipotency. (pubmedcentralcanada.ca)
  • MYO1E-null tumors exhibited decreased expression of the markers of cell proliferation, which was recapitulated in primary tumor cells derived from MYO1E-null mice. (oncotarget.com)
  • Understanding how mature CMs can disassemble their sarcomeric architecture and re-enter the cell cycle, combined with the development of novel procedures that can facilitate CM dedifferentiation and proliferation, are major challenges facing current biomedical research. (weizmann.ac.il)
  • After the ablation of airway stem cells, we observed a surprising increase in the proliferation of committed secretory cells. (jijitang.com)
  • thus oligodendroglial dedifferentiation is dependent on proliferation. (elsevier.com)
  • During dedifferentiation, adipocytes passed the stages of cytoplasmic outgrowths, multi-drop adipocytes, the proliferation of fat-containing cells, and a confluent monolayer. (radiotec.ru)
  • Sugihara H., Yonemitsu N., Miyabara S., Toda S. Proliferation of unilocular fat cells in the primary culture // Journal of Lipid Research. (radiotec.ru)
  • In model organisms, regeneration usually occurs as the induction of proliferation in differentiated cells. (bioportfolio.com)
  • Together, the data suggest that rather than inducing proliferation of mature cells, developmental pathways are reinstated, and that a pool of naïve progenitors at the blastema site forms the basis for this regeneration. (bioportfolio.com)
  • Proposed resistance mechanisms include T-cell exhaustion or immune tolerance, and selection for T cells with genetic alterations that increase proliferation and result in loss of the target antigen. (aacrjournals.org)
  • An autoradiographic study using tritiated Thymidine to follow cell proliferation and migration, Develop. (springer.com)
  • Cancer molecular biology, with special emphasis on the role of the p53 tumor suppressor, regulation of cell proliferation and cell death, regulation and function of microRNAs, and the role of chromatin modifications in cancer. (weizmann.ac.il)
  • Hepatocyte growth factor/hepatopoietin A stimulates the growth of rat kidney proximal tubule epithelial cells (RPTE), rat nonparenchymal liver cells, human melanoma cells, mouse keratinocytes and stimulates anchorage-independent growth of SV-40 transformed RPTE," Biochemical and Biophysical Research Communications , vol. 174, no. 1, pp. 331-337, 1991. (hindawi.com)
  • I am studying epithelial cells and do notice some morphological changes after treatment but don't know if they are characteristic of dedifferentiation. (protocol-online.org)
  • Hi, What is the source of your epithelial cell? (protocol-online.org)
  • In this study, we introduce NPM-ALK into primary human T cells and demonstrate induction of the epithelial-to-mesenchymal transition (EMT) program, attenuation of most T cell effector programs, re-emergence of an immature epigenomic profile, and dynamic regulation of c-Myc, E2F, and PI3K/mTOR signaling pathways early during transformation. (aacrjournals.org)
  • Human corneal epithelial cells were cultivated from fresh limbal tissue explants in two conditions: high calcium serum-containing SHEM and a defined, low calcium and serum free medium (CnT-20). (arvojournals.org)
  • Corneal epithelial cells harvested from explant cultures in the low calcium serum-free defined medium CnT-20 generated 2 fold colony forming efficiency compared with those cultured in SHEM. (arvojournals.org)
  • Further study demonstrated concomitant up-regulation of TCF4, DLL-1, GAS-1, p57 and NGF genes expression levels in more stem-like corneal epithelial progenitor cells. (arvojournals.org)
  • These findings demonstrated that human corneal epithelial cells cultured in the defined media present stem-like progenitor cell characteristics. (arvojournals.org)
  • What if, after transformation of breast duct epithelial cells, angiogenesis (the growth of blood vessels) is PROGRAMMED to lag behind tumor growth, so that as oxygen tension goes down in the tumor, it's cells increasingly DEDIFFERENTIATE. (ipscell.com)
  • undergoing EMT (epithelial to mesenchymal transfer) to become cancer stem cells. (ipscell.com)
  • Then as these cells with a mesenchymal phenotype crawl toward the blood vessels to metastasise they are exposed to increased oxygen tension and revert to an epithelial phenotype? (ipscell.com)
  • This is a repeat of what happens to the normal mesenchymal cells that sit below the normal epithelial cells and presumably are in a more hypoxic environment and thus more 'stemmie', a precursor to the epithelial cell. (ipscell.com)
  • Come ovulation and the need for a lot more epithelial cells, then perhaps a vasodilating prostaglandin increases the oxygen tension bathing the mesenchymal cells just below the epithelia and they divide whilst differentiating INTO epithelia. (ipscell.com)
  • Tumor latency in mice lacking MYO1E was significantly increased, and tumors formed in the absence of MYO1E displayed unusual papillary morphology, with well-differentiated layers of epithelial cells covering fibrovascular cores, rather than solid sheets of tumor cells typically observed in this cancer model. (oncotarget.com)
  • Here we present evidence that differentiated airway epithelial cells can revert into stable and functional stem cells in vivo. (jijitang.com)
  • Dedifferentiated cells were morphologically indistinguishable from stem cells and they functioned as well as their endogenous counterparts in repairing epithelial injury. (jijitang.com)
  • Early in pancreatic development, epithelial cells of pancreatic buds function as primary multipotent progenitor cells (1°MPC) that specify all three pancreatic cell lineages, i.e., endocrine, acinar and duct. (harvard.edu)
  • The miR-30 family microRNAs confer epithelial phenotype to human pancreatic cells. (biomedsearch.com)
  • We show here for the first time that reduction in miR-30 family microRNAs, is responsible for mesenchymal transition of primary cultures of human pancreatic epithelial cells. (biomedsearch.com)
  • Together, our data confirm that miR-30 family microRNAs form a part of the regulatory signaling events involved in cellular response of pancreatic epithelial cells during mesenchymal transition. (biomedsearch.com)
  • I described the results as these genomes looking as though someone threw a miniature grenade into the nucleus of a prostate epithelial cell. (scienceblogs.com)
  • Retinal pigment epithelial (RPE) cells maintain the health and functional integrity of both photoreceptors and the choroidal vasculature. (frontiersin.org)
  • Taken together, these data suggest that RPE cells retain the reprogramming capacity to move along a continuum between polarized epithelial cells and mesenchymal cells. (frontiersin.org)
  • Epithelial cells and mesenchymal cells exhibit different characteristics and functions in the human body. (frontiersin.org)
  • Major hallmarks of terminally differentiated epithelial cells are expression of junctional complexes and apical-basal polarization. (frontiersin.org)
  • Epithelial cells reside on a basal membrane that separates them from other tissue components. (frontiersin.org)
  • In contrast to epithelial cells, mesenchymal cells are non-polarized cells with invasive and migratory behavior. (frontiersin.org)
  • In healthy tissues, fully differentiated epithelial cells typically exert specific functions after development and are thought not to oscillate between two states. (frontiersin.org)
  • However, EMT can be activated under pathological circumstances, such as inflammation, wound healing, and carcinogenesis, enabling epithelial cells to obtain an enhanced migration ability and increase their production of extracellular matrix components. (frontiersin.org)
  • The adult newt can regenerate lens from pigmented epithelial cells (PECs) of the dorsal iris via dedifferentiation. (molvis.org)
  • It is well known that lens regeneration is mediated by dedifferentiation and transdifferentiation of terminally differentiated pigmented epithelial cells (PECs). (molvis.org)
  • Our data show that the WOUND INDUCED DEDIFFERENTIATION3 (WIND3) and LEAFY COTYLEDON2 (LEC2) genes are among the PRC2 targets involved in this reprogramming, as their ectopic overexpression partly phenocopies the dedifferentiation phenotype of PRC2 mutants. (nih.gov)
  • Our results indicate that CSC phenotype is dynamic and may be acquired through dedifferentiation and Oct4 is a positive regulator of tumor dedifferentiation. (aacrjournals.org)
  • MAIN OUTCOME MEASURES Immunohistochemical evaluation of smooth muscle cell phenotype modulation in relation to proliferative activity. (bmj.com)
  • 3-6 Unni and colleagues 4 documented insudation of blood constituents into the vein intima as the earliest change, whereas Kockx and colleagues 6 showed that less than 10 days after grafting, veins contained smooth muscle cells (SMCs) in the media with reduced expression of α smooth muscle actin, suggesting a synthetic phenotype. (bmj.com)
  • Indeed, growing evidence suggests that under certain conditions, mature beta-cells can lose, to various degrees, their differentiated phenotype and cellular identity and regress to a less differentiated or a precursor-like state. (garvan.org.au)
  • Dedifferentiation can change the phenotype of specialized cells, rendering them closer to their ancestors with augmented plasticity. (pubmedcentralcanada.ca)
  • Cell viability was measured by MTT colorimetric assay and the conversion of myofibroblast into quiescent fat-storing cell phenotype was evaluated by Oil-Red-O staining . (bvsalud.org)
  • SNAC induced phenotype conversion of myofibroblast -like phenotype into quiescent cells . (bvsalud.org)
  • Mia cells, which are less resistance to the standard drug used for pancreatic cancer, gemcitabine, expressed higher levels of GATA6 compared to Panc1, a more resistance phenotype. (aacrjournals.org)
  • We now show that this effect is direct and not mediated through the effects of bFGF on other cell types, because we were able to demonstrate similar changes in oligodendrocyte phenotype in enriched oligodendrocyte cultures prepared by immunopanning. (elsevier.com)
  • Concordantly, several pancreatic progenitor cell-specific transcription factors like SOX4, SOX9, and ID2 were upregulated in cultured islets, suggesting progressive transformation of mature beta-cell phenotype toward an immature endocrine cell phenotype. (duhnnae.com)
  • Our findings suggest islet isolation and culture induces an inflammatory response and loss of the mature endocrine cell phenotype. (duhnnae.com)
  • A better understanding of the signals required to maintain a mature beta-cell phenotype may help improve the efficacy of islet transplantation. (duhnnae.com)
  • Much of our understanding comes from rodent models in which the phenotype of β cells from wild-type and transgenic mice and rats was analyzed first in vivo and, once islet isolation procedures were established ( 6 ), expanded upon in vitro. (jci.org)
  • The process of dedifferentiation was also confirmed by single cell clonal analysis. (eurekamag.com)
  • To understand the process of dedifferentiation, analysis of global gene expression during dedifferentiation is needed. (molvis.org)
  • Human multipotent stromal cells/mesenchymal stem cells (MSCs) are cells of mesenchymal origin, which are cultured as adherent monolayers in vitro. (bl.uk)
  • These early embryo-like cells resembled human early embryo derived from in vitro fertilization (IVF) in morphology, and hatching activity. (ijbs.com)
  • This study provides a simple model to derive early human embryo-like cells by in vitro . (ijbs.com)
  • Recent in vivo and in vitro studies of β-cell biology have established that the β-cell loses differentiated characteristics, including glucose-mediated insulin secretion, under metabolic stress. (diabetesjournals.org)
  • The recent in vivo and in vitro studies will be reviewed, together with the evidence that the underlying, potentially reversible, β-cell failure is related to dedifferentiation rather than to β-cell death. (diabetesjournals.org)
  • Some, but not all, MDCs self-organized into spheres and re-differentiated into myocytes and endothelial cells in vitro . (pubmedcentralcanada.ca)
  • Characteristically, adipocytes induced in vitro were involved in dedifferentiation earlier, and the efficiency of transformation was 1.6 times higher than that of primary adipocytes. (radiotec.ru)
  • Over the past decades, β cell protection has been extensively investigated in rodents both in vivo and in vitro using isolated islets or rodent β cell lines. (jci.org)
  • Further, coculturing F5 TCR transduced T cells with human melanoma cell lines was sufficient to induce their dedifferentiation in vitro , inducing downregulation of MART1 and upregulation of NGFR. (aacrjournals.org)
  • Subsequently, in vitro and in vivo assays for clonogenic human stem and progenitor hematolymphoid cells were developed and by the same approach candidate human HSCs were isolated ( 16 , 17 ). (sciencemag.org)
  • These attributes are complemented by sophisticated, clinically relevant in vitro and in vivo models of the trauma impact and resulting complications to gain deep insights into the mechanistic complexity and regeneration potential post trauma as well as to evaluate innovative cell- and molecular based therapies. (uni-ulm.de)
  • Although the ability of engrafted stem cells to regenerate tissue has received much attention, the molecular mechanisms controlling regeneration are poorly understood. (sciencemag.org)
  • While the major role of glucotoxicity in beta-cell dedifferentiation is well established, the precise mechanisms involved are still under investigation. (garvan.org.au)
  • This review highlights the identified molecular mechanisms implicated in beta-cell dedifferentiation including oxidative stress, endoplasmic reticulum (ER) stress, inflammation and hypoxia. (garvan.org.au)
  • Now that weight loss has been shown to bring about restoration of β-cell function and reversal of diabetes, a fundamental reappraisal of the mechanisms underlying β-cell dysfunction is required. (diabetesjournals.org)
  • Stem cells from patients will provide various tools in understanding disease mechanisms, searching for effective drugs, and evaluating toxicity of drug candidates. (keystonesymposia.org)
  • OBJECTIVE This article examines the foundation of β-cell failure in type 2 diabetes (T2D) and suggests areas for future research on the underlying mechanisms that may lead to improved prevention and treatment. (diabetesjournals.org)
  • This research enables us to ask questions about the mechanisms that maintain stem-cell pluripotency and the ways that cells make fate decisions within the immune system. (sanger.ac.uk)
  • The two most well-studied mechanisms of retinal regeneration are cell-mediated regeneration and cellular transplantation. (wikipedia.org)
  • The differential responses observed in β cells together with the heterogeneity in disease phenotypes within the patient population emphasize the need to better understand the mechanisms behind β cell adaptation, identity, and dysfunction in MetS. (jci.org)
  • In this Review, we will discuss the well-characterized mechanisms that contribute to disease-mediated β cell dysfunction and death, as well as potential alternative β cell adaptive responses to external stressors associated with MetS based on recent studies in mice and evidence from human cadaveric pancreas tissue. (jci.org)
  • This review summarizes recently published data on the mechanisms of tumor cell interaction with the tumor microenvironment. (chemweb.com)
  • In an attempt to get insight into molecular mechanisms involved in control of cell activation and dedifferentiation in the regenerating echinoderm CNS, we investigated expression patterns of pluripotency factors in the RNC of a sea cucumber at various time points after injury [ 6 , 7 ]. (biomedcentral.com)
  • However, the mechanisms underlying such dedifferentiation remain poorly understood. (jneurosci.org)
  • What are the mechanisms regulating the interactions of senescent cells with their microenvironment? (weizmann.ac.il)
  • Nevertheless, integrated and coordinated mechanisms of subversion of NK cell activity against the tumor and its microenvironment exist. (frontiersin.org)
  • IL-6, corticosteroids) in tissue regeneration, and defining mobilisation/homing mechanisms and the therapeutic potential of stem cells to improve healing processes after severe trauma. (uni-ulm.de)
  • Plant somatic cells are generally acknowledged to retain totipotency, the potential to develop into any cell type within an organism. (nih.gov)
  • Here we show that POLYCOMB REPRESSIVE COMPLEX 2 (PRC2), a chromatin regulator that maintains gene repression through histone modification, prevents dedifferentiation of mature somatic cells in Arabidopsis thaliana roots. (nih.gov)
  • Loss-of-function mutants in PRC2 subunits initially develop unicellular root hairs indistinguishable from those in wild type but fail to retain the differentiated state, ultimately resulting in the generation of an unorganized cell mass and somatic embryos from a single root hair. (nih.gov)
  • In the Drosophila testis, germline stem cells (GSCs) attach to a cluster of quiescent somatic cells called the hub ( Fig. 1A ), which creates a special localized microenvironment, or niche, by producing the ligand Unpaired (Upd). (sciencemag.org)
  • Cyst cells (gray), produced by somatic stem cells (gray, at the hub) envelop GBs and form cysts. (sciencemag.org)
  • a spermatocyte cyst (outlined, large arrowhead) and a few Vasa-negative cells (somatic stem cells or cyst cells, small arrowhead) remain. (sciencemag.org)
  • Reprogramming factors (protein, RNAs and small molecules) enables somatic cells to reprogram [ 3 , 4 ]. (ijbs.com)
  • The H3K27me3 is a repressive chromatin marker in dedifferentiating somatic cells to iPSCs. (ijbs.com)
  • The H3K27me3 domains in iPSC are smaller, interspersed, and spanning less base pairs as compared with somatic cells [ 14 ]. (ijbs.com)
  • Three basic categories of cells make up the mammalian body: germ cells, somatic cells, and stem cells. (wikipedia.org)
  • Such cells, called somatic cells, make up most of the human body, such as skin and muscle cells. (wikipedia.org)
  • Induced pluripotent stem cells (iPSC), generated by introduction of a few defined factors in a somatic cell, provide an ideal patient-specific source for disease modeling, drug discovery and cellular therapies. (stanford.edu)
  • We have shown that, surprisingly, somatic ablation of Foxo1 in Neurog3 + enteroendocrine progenitor cells gives rise to gut insulin-positive cells (Ins + ) that express markers of mature-cells, and secrete bioactive insulin as well as C-peptide in response to glucose and sulfonylureas. (nbdiabetes.org)
  • In the fruit fly testes, germ line stem cells attach to a cluster of non-dividing somatic cells called the hub. (hopkinsmedicine.org)
  • They are also determining if the hub directly activates Stat in somatic stem cells, or if a different mechanism is operating to ensure their renewal, since little is known of how two stem cell populations are regulated within one niche. (hopkinsmedicine.org)
  • Hetie P, de Cuevas M, Matunis E. "Conversion of quiescent niche cells to somatic stem cells causes ectopic niche formation in the Drosophila testis. (hopkinsmedicine.org)
  • and (iii) progenitor cell markers. (columbia.edu)
  • and (3) the likely upregulation of progenitor cell genes. (garvan.org.au)
  • Here we test the hypothesis that mature mammalian cardiomyocytes retain substantial cellular plasticity, including the ability to dedifferentiate, proliferate, and acquire progenitor cell phenotypes. (pubmedcentralcanada.ca)
  • In response to photoreceptor ablation, Muller glia dedifferentiate and undergo a single asymmetric division to produce a neural progenitor cell and a new Muller glia cell. (wikipedia.org)
  • The neural progenitor cell proliferates to form a cluster of neural progenitors, which migrate to the outer nuclear layer of the retina and differentiate into photoreceptors to replace the lost cells. (wikipedia.org)
  • The clinical application of stem and progenitor cell transplantation began with the exposure of civilian populations to lethal doses of radiation in 1945. (sciencemag.org)
  • The Polycomb-Dependent Epigenome Controls β Cell Dysfunction, Dedifferentiation, and Diabetes. (nih.gov)
  • We find two chromatin-state signatures that track β cell dysfunction in mice and humans: ectopic activation of bivalent Polycomb-silenced domains and loss of expression at an epigenomically unique class of lineage-defining genes. (nih.gov)
  • We have shown that mitochondrial abnormalities are a key step in the progression of β-cell dysfunction towards dedifferentiation. (nih.gov)
  • Up-regulation of PPAR γ , heat shock protein-27 and -72 by naringin attenuates insulin resistance, β -cell dysfunction, hepatic steatosis and kidney damage in a rat model of type 2 diabetes," British Journal of Nutrition , vol. 106, no. 11, pp. 1713-1723, 2011. (hindawi.com)
  • The goal of our work is to understand the integrated physiology of insulin resistance and β-cell dysfunction to define potential sites and modes of intervention against diabetes ( 1 ). (diabetesjournals.org)
  • Multiple pathways underlie decreased β-cell function and mass, some of which may be shared and may also be a consequence of processes that initially caused dysfunction. (diabetesjournals.org)
  • In October 2013, the Global Partnership to Accelerate Diabetes Research, cosponsored by the Endocrine Society and the American Diabetes Association, assembled international experts (see appendix ) to inform the global health research agenda by reviewing the state of the science and identifying pressing research needs related to β-cell dysfunction in T2D. (diabetesjournals.org)
  • They found two chromatin-state "signatures" that are well correlated with beta-cell dysfunction - both in mice and in humans) - [a] ectopic activation of bivalent Polycomb-silenced domains, and [b] loss of expression at an epigenomically unique class of lineage-defining genes. (genewhisperer.com)
  • The observation that the degree of β cell dysfunction correlates with the severity of MetS highlights the need to better understand β cell dysfunction in the development of MetS. (jci.org)
  • however, chronic exposure to excess glucose and lipids eventually leads to β cell dysfunction and/or cell death to cause overt diabetes. (jci.org)
  • These findings suggest that understanding β cell dysfunction in MetS may inform novel approaches to treating T2DM, cardiovascular disease, and other associated MetS complications. (jci.org)
  • Schematic representation of infarction (cell death), chronic ischemia with contractile dysfunction (hibernating myocardium), and transient ischemia (stunned myocardium) with restored blood flow with transient contractile dysfunction. (medscape.com)
  • The work provides novel resources for exploring β cell transcriptional regulation and identifies PRC2 as necessary for long-term maintenance of β cell identity. (nih.gov)
  • Regulation of cell adhesion and anchorage-dependent growth by a new β 1-integrin-linked protein kinase," Nature , vol. 379, no. 6560, pp. 91-96, 1996. (hindawi.com)
  • Integrin-linked kinase (ILK) modulates wound healing through regulation of hepatocyte growth factor (HGF)," Experimental Cell Research , vol. 318, no. 19, pp. 2470-2481, 2012. (hindawi.com)
  • Later relapses had loss of all the B cell surface markers, along with down regulation of B cell transcripts (such as EBF1 and PAX5) in the mRNA. (nih.gov)
  • Significance: MUC1-C directly interacts with MYC to activate the NuRD complex, mediating regulation of the estrogen receptor in triple-negative breast cancer cells. (elsevier.com)
  • As advances in our understanding of insulin action and β-cell failure reach a critical stage, here I draw on lessons learned from our research on insulin regulation of gene expression and pancreatic β-cell dedifferentiation to address the question of how we can translate this exciting biology into mechanism-based interventions to reverse the course of diabetes. (diabetesjournals.org)
  • These breakthrough experiments provide a new direction for exploring beta-cell transcriptional regulation and identify PRC2 as a necessary polycomb-repressive complex for long-term maintenance of beta-cell identity. (genewhisperer.com)
  • At the chromatin level, aging associates with progressive accumulation of epigenetic errors that eventually lead to aberrant gene regulation, stem cell exhaustion, senescence, and deregulated cell/tissue homeostasis. (stanford.edu)
  • Specific research topics: Regulation of p53 activity in normal & cancer cells. (weizmann.ac.il)
  • For cells to change their identity and assume a new fate, a considerable degree of gene regulation must take place. (molvis.org)
  • By studying stem cells that sustain spermatogenesis in the fruit fly Drosophila melanogaster, Dr. Matunis' lab has begun to understand stem cell regulation. (hopkinsmedicine.org)
  • Generation of human induced pluripotent stem cells from dermal fibroblasts," Proceedings of the National Academy of Sciences of the United States of America , vol. 105, no. 8, pp. 2883-2888, 2008. (hindawi.com)
  • Mitochondria underwent remodelling to a small, rounded morphology, highly similar to mitochondria observed in pluripotent cells. (bl.uk)
  • Recent progress in induced pluripotent stem (iPS) cells has shown that stem cells can be generated from differentiated progenies by introducing just a few defined factors. (keystonesymposia.org)
  • Of the large number of TFs expressed in a neural-lineage cell line, we identified a subset of TFs that, when overex-pressed, strongly interfered with the dedifferentiation triggered by the procedure to generate induced pluripotent stem cells. (elsevier.com)
  • In plants, there are specific cells that are located at the site of such wounds that retain the ability to return to a pluripotent state. (wikipedia.org)
  • A cell that can differentiate into all cell types of the adult organism is known as pluripotent. (wikipedia.org)
  • Such cells are called meristematic cells in higher plants and embryonic stem cells in animals, though some groups report the presence of adult pluripotent cells. (wikipedia.org)
  • Virally induced expression of four transcription factors Oct4, Sox2, c-Myc, and Klf4 (Yamanaka factors) is sufficient to create pluripotent (iPS) cells from adult fibroblasts. (wikipedia.org)
  • These cells are referred to as pluripotent. (wikipedia.org)
  • Pluripotent stem cells undergo further specialization into multipotent progenitor cells that then give rise to functional cells. (wikipedia.org)
  • I strongly believe in stem cells and induced pluripotent cells as the future of regenerative medicine, yet we first must understand how stem cells direct themselves to their final differentiated state within the complex setting of any tissue or organ. (clarkson.edu)
  • Fusion genes including NPM-ALK can promote T cell transformation, but the signals required to drive a healthy T cell to become malignant remain undefined. (aacrjournals.org)
  • When cells originally grown in SHEM were sub-cultured to CnT-20, stem cell associated genes expression levels were up-regulated and the cells could be continually passaged 4 times in CnT-20 medium. (arvojournals.org)
  • Interestingly, gene profiling and bioinformatics demonstrated that upregulation ( DHCR 24, G 0 S 2, MAP 2 K 6, SESN 3) and downregulation ( DST , KAT 2, MLL 5, RB 1, SMAD 3, ZA K) of distinct genes have an association with cell cycle arrest in adipogenic-differentiated cells and perhaps narrow down the lineage potency. (eurekamag.com)
  • However, the upregulation (CCND1, CHEK, HGF , HMGA 2, SMAD 3) and downregulation ( CCPG 1, RASSF 4, R GS 2) of these genes have an association with cell cycle progression and maybe motivate dedifferentiation of adipogenic-differentiated cells. (eurekamag.com)
  • Concluded, our results indicate that transdifferentiation of adipogenic-differentiated cells into osteogenic- or chondrogenic-differentiated cells proceeds via dedifferentiation and correlates with cell cycle arresting and deriving genes. (eurekamag.com)
  • 61 differentially expressed genes in adipogenic-differentiated cells have an association with cell cycle arrest. (eurekamag.com)
  • 65 differentially expressed genes in dedifferentiated cells have an association with cell cycle progression. (eurekamag.com)
  • Genome-wide association studies indicate that the vast majority of the genes associated with type 2 diabetes are likely to have a β-cell-specific role relating to impaired ability to cope with metabolic stress ( 17 ). (diabetesjournals.org)
  • MUC1-C/MYC complexes selectively activated the MTA1 and MBD3 genes and posttranscriptionally induced CHD4 expression in basal- but not luminal-type BC cells. (elsevier.com)
  • For instance, quail pigment cells derived from neural crest can dedifferentiate to become multipotent self-renewing progenitors expressing early neural marker genes Sox10, FoxD3, Pax3 and Slug, and give rise to glial cells and myofibroblasts [10] . (pubmedcentralcanada.ca)
  • Using a human embryonic stem cell RT2 Profiler gene array, numerous stem cell genes expressed in pancreatic cancer cell lines (MIAPaca2, Panc1) can be identified. (aacrjournals.org)
  • This study has shown that the dietary agent, I3C, alone and in combination with metformin down-regulate critical stem cell genes involved in dedifferentiation of cancer cells that should affect the maintenance of their pluripotency and self-renewal. (aacrjournals.org)
  • genes discovered through family-based approaches are important regulators of insulin secretion and β-cell development. (diabetesjournals.org)
  • The activation of the transcription factor NF-κB leads to changes in expression of many genes in pancreatic β-cells. (harvard.edu)
  • But at the individual cell level, there is tremendous variability, and for most genes, very little activity at any one time. (ucl.ac.uk)
  • We have reported that ablation of the three genes in vascular endothelial cells prevents atherosclerosis in Low-density lipoprotein receptor knockout mice. (nbdiabetes.org)
  • Microarray profiling coupled with Ingenuity Pathway Analysis revealed that reversine treatment resulted in a significant expression change in many genes including those required for cell-cell interaction, cell movement, cell growth, and development. (bjmu.edu.cn)
  • The presence of several cancer- and apoptosis-related genes during newt dedifferentiation was revealed. (molvis.org)
  • Moreover, several candidate genes, which might participate in reprogramming during dedifferentiation, were also found. (molvis.org)
  • The expression of cancer- and apoptosis-related genes could be hallmarks during dedifferentiation. (molvis.org)
  • Using genetic and genomic approaches, the Matunis lab continues to identify more genes that define the germ line stem cells' fate. (hopkinsmedicine.org)
  • Finally, it proposes a novel hypothesis of beta-cell dedifferentiation as a potential adaptive mechanism to escape cell death under stress conditions. (garvan.org.au)
  • Lastly, we identified SOX9 targets in human β cells as potentially new markers of dedifferentiation in T1D. (jci.org)
  • Human and murine diabetes is characterized by pancreatic β-cell dedifferentiation, a process in which β-cells lose expression of markers of maturity and gain those of endocrine progenitors. (columbia.edu)
  • These late relapses showed expression of cell surface markers compatible with a myeloid lineage (Gr1, Mac1), T cell or undifferentiated lineage (KIT, CD9), confirmed by RNA-sequencing. (nih.gov)
  • These cells also expressed hypoblast (GATA4) and trophoblast (Cdx2) markers. (ijbs.com)
  • The relapsed tumor exhibited increased CD8 + cytotoxic T-lymphocyte infiltration, consistent with increased inflammatory signals in the tumor microenvironment, and expression of inflammation-induced dedifferentiation markers including NGFR was increased. (aacrjournals.org)
  • Click on a cell type below to view a subset of molecules used as markers for the identification of that cell type. (rndsystems.com)
  • In vivo, mice with β-cell-specific ablation of Cyb5r3 (B-Cyb5r3) display impaired glucose tolerance with decreased insulin secretion, and their islets have significantly lower basal respiration and glucose-stimulated insulin secretion. (columbia.edu)
  • At 11-12 days of culture the cells reaggregated into clumps, began to contract spontaneously, and formed into well-organized muscle bundles in two layers at right angles, resembling the muscle layer organization of the in vivo vas deferens. (biologists.org)
  • Single secretory cells clonally dedifferentiated into multipotent stem cells when they were cultured ex vivo without basal stem cells. (jijitang.com)
  • In the long-term perspective, a valid functional immune monitoring of the individual danger response on the background of various co-morbidities and adapted immune- and cell-based therapies, for example by complement intervention and large-scale GMPgrade cellular ex vivo expansion, are anticipated. (uni-ulm.de)
  • Reprogramming of fibroblasts and mesenchymal stem cells (MSCs) is a promising strategy for stem cell biology and regenerative medicine. (ijbs.com)
  • Sarah's research combines expertise in computational and systems biology with single-cell biology, genomics, structural biology and immunology. (sanger.ac.uk)
  • A series of rodent β cell lines developed since the 1970s have further benefited β cell biology. (jci.org)
  • With the use of next generation sequencing, bioinformatics and advanced cell biology approaches, the lab aims to address how chromatin is dynamicaly regulated by environmental and intrinsic signals to control cellular decisions and development. (dur.ac.uk)
  • From the basic biology perspective, these findings have revealed that cell fates are inherently plastic and are dynamically regulated. (stanford.edu)
  • Chromatin modifications and cancer, with emphasis on histone H2B ubiquitination as a new regulator of cancer-related processes and of stem cell biology. (weizmann.ac.il)
  • In this article, we discuss the current knowledge on how NK cells recognize and kill cancer cells while sparing the normal healthy cells, how the cancer and its associated cells exploit this biology. (frontiersin.org)
  • Dr. Erika Matunis is a professor of cell biology at the Johns Hopkins University School of Medicine. (hopkinsmedicine.org)
  • Matunis EL, Stine RR, de Cuevas M. "Recent advances in Drosophila male germline stem cell biology. (hopkinsmedicine.org)
  • However, current results imply that even terminally differentiated cells transdifferentiate across lineage boundaries and therefore act as a progenitor cells for other lineages. (eurekamag.com)
  • The authors show that mature neurons and astrocytes (incredibly specialized, "terminally differentiated" cells) can be dedifferentiated. (ipscell.com)
  • Conversion of one terminally differentiated cell type into another (or transdifferentiation) usually requires the forced expression of key transcription factors. (harvard.edu)
  • Dedifferentiation derived cells exhibit phenotypic and functional characteristics of epidermal stem cells," Journal of Cellular and Molecular Medicine , vol. 14, no. 5, pp. 1135-1145, 2010. (hindawi.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • We now seek to understand the molecular and cellular underpinnings of this phenomenon using adult muscle stem cells. (weizmann.ac.il)
  • Single-cell transcriptomes from human kidneys reveal the cellular identity of renal tumors. (sanger.ac.uk)
  • The teams are using single-cell approaches, so called "cell atlas" technologies to combined with human genetics at the cellular level. (sanger.ac.uk)
  • Main cellular components of hepatocellular carcinoma (HCC) stroma are tumor-associated fibroblasts, hepatic stellate cells, immune cells, and endothelial cells that produce extracellular components of tumor microenvironment such as extracellular matrix, various proteins, proteolytic enzymes, growth factors, and cytokines. (chemweb.com)
  • Here we demonstrate that knockdown of Myc inhibits dedifferentiation of radial glia and programmed cell death, the two most prominent cellular events that take place in the regenerating sea cucumber nervous system shortly after injury. (biomedcentral.com)
  • Efficient dedifferentiation is characterized by robustness to mutation, flexibility in ordering of cellular events and reversal of developmental changes along a single gene expression trajectory. (elifesciences.org)
  • Plant cells have a remarkable plasticity that allows cellular reprogramming from differentiated cells and subsequent tissue regeneration. (bioportfolio.com)
  • Further analysis revealed that there was involvement of gene networks concerned with cellular assembly and organization, DNA replication and repair, tissue morphology, and cell-to-cell signaling. (bjmu.edu.cn)
  • After lens removal, PECs in dorsal irises undergo dedifferentiation where PECs exclude pigment granules and lose their cellular identity, proliferate, and then differentiate into lens cells. (molvis.org)
  • Hanan Hosni Ahmed, Laila Ahmed Rashed, Sohair Mahfouz, Rania Elsayed Hussein, Marwa Alkaffas, Shaimaa Mostafa, and Azza Abusree, "Can mesenchymal stem cells pretreated with platelet-rich plasma modulate tissue remodeling in a rat with burned skin? (hindawi.com)
  • In several lines of human endometrial polyp- and cervical polyp-mesenchymal stem cells (EPMSCs and CPMSC), we showed introduction of the four transcription factors led to a dedifferentiation of these cells into early embryo-like cells in three days, ranging from one-cell, two-cell, four-cell embryos, and morula to blastocyst. (ijbs.com)
  • Cancer stem cells (CSCs) exhibit mesenchymal stem cell (MSC) features and represent a subpopulation of tumor cells that play an important role in tumor progression, chemotherapy resistance, recurrence and metastasis. (mdpi.com)
  • However, frequently CSC subpopulations emerge after the accumulation of epigenetic and/or genetic changes in a cell within the aberrant population, initially generated by the cell of origin, namely, mesenchymal stem cell (MSC)-derived cell types [ 2 ]. (mdpi.com)
  • As RPE cells become less differentiated, they begin to exhibit the defining characteristics of mesenchymal cells, namely, the capacity to migrate and proliferate. (frontiersin.org)
  • Thus, in the appropriate microenvironment, transit-amplifying cells dedifferentiate, becoming functional stem cells during tissue regeneration. (sciencemag.org)
  • Hence, another angle of our research focus on the pros and cons of cell-cycle re-entry, finding just the right balance between these two processes (regeneration and tumorigenesis). (weizmann.ac.il)
  • This capacity of committed cells to dedifferentiate into stem cells may have a more general role in the regeneration of many tissues and in multiple disease states, notably cancer. (jijitang.com)
  • Retinal regeneration in zebrafish is mediated by Muller glia, which dedifferentiate into stem-like cells and proliferate into neural progenitor cells in response to retinal damage. (wikipedia.org)
  • The ability of the echinoderm glia to acquire pluripotency and take on a leading role in neural regeneration contrasts with the early post-traumatic events in mammals, where glial cells form a scar, which inhibits any subsequent regeneration across the wound gap [ 4 , 5 ]. (biomedcentral.com)
  • Stem cells are the natural units of embryonic generation, and also adult regeneration, of a variety of tissues. (sciencemag.org)
  • Dedifferentiation is the ability of plants to regain and develop into specific cell types required for regeneration. (wikipedia.org)
  • Vertebrate limb regeneration involves cell dedifferentiation and growth. (mun.ca)
  • The purpose of this research is to obtain sequence resources for a newt lens regeneration study and to obtain insights of dedifferentiation at the molecular level. (molvis.org)
  • 1,368 contigs and 3,357 singlets) from the iris undergoing dedifferentiation during the process of lens regeneration and analyzed their expression profiles. (molvis.org)
  • A normal β-cell can adapt its mitochondrial fuel source based on substrate availability, a concept known as "metabolic flexibility. (nih.gov)
  • We propose that impaired mitochondrial function marks the progression from metabolic inflexibility to dedifferentiation in the natural history of β-cell failure. (columbia.edu)
  • To advance diabetes treatment and to work toward a cure, a better understanding of how to protect the pancreatic β cells against autoimmune or metabolic assaults (e.g., obesity, gestation) will be required. (jci.org)
  • We undertook this study to examine whether viral infection could induce human β cell dedifferentiation. (jci.org)
  • Potent NF-κB activators induce SOX9 expression in human β cells. (jci.org)
  • The paper, entitled " Dedifferentiation of Neurons and Astrocytes by Oncogenes Can Induce Gliomas in Mice " makes striking findings. (ipscell.com)
  • Strikingly, the maintenance activity of the interfering TF set was strong enough to induce the cell line-specific transcriptional profile when overexpressed in a heterologous cell type. (elsevier.com)
  • Furthermore, my research using MIN6 and db/db also identified a novel druggable compound ANJR12947285, which has the potential to induce redifferentiation in dedifferentiated β-cell. (edu.au)
  • This effect was mediated by TNFα released by T cells, and TNFα alone was sufficient to induce reversible dedifferentiation of melanoma cells. (aacrjournals.org)
  • Ultrastructurally, the characteristic secretory granules in the neoplastic cells decreased remarkably in number, consistent with the immunohistochemical findings. (springer.com)
  • These findings unveil the pivotal role of PRC2-mediated gene repression in preventing unscheduled reprogramming of fully differentiated plant cells. (nih.gov)
  • These findings reveal that inflammatory signaling has clear implications in human β cell dedifferentiation. (jci.org)
  • These findings support a model in which MUC1-C activates the NuRD complex to drive dedifferentiation and reprogramming of TNBC cells. (elsevier.com)
  • In conclusion, these findings demonstrate that SNAC efficiently can modulate the activation and functionality of murine hepatic stellate cells and could be considered as an antifibrotic treatment to human liver fibrosis . (bvsalud.org)
  • In findings presented in the journal " Proceedings of the National Academy of Sciences " in 2012, a Nuffield Laboratory of Ophthalmology research team led by Dr Robert MacLaren from the University of Oxford restored sight to totally blind mice by injections of light-sensing cells into their eyes. (wikipedia.org)
  • This Review focuses on the current understanding from rodent and human studies of the progression of β cell responses during the development of MetS, as well as recent findings addressing the complexity of β cell identity and heterogeneity within the islet during disease progression. (jci.org)
  • These findings challenge the traditional glioma cell heterogeneity model, cell division model and glioma malignancy development model. (oncotarget.com)
  • Clinically, these findings have uncovered the possibility of unprecedented sources for patient-autologous cells with far reaching implications in a variety of diseases. (stanford.edu)
  • This is reinforced by findings of decreased NK-cell activity among relatives of patients diagnosed with familial melanoma ( 11 ), implicating NK cells in tumor surveillance. (frontiersin.org)
  • TOR (focus on of rapamycin) can be an evolutionarily conserved nutrient sensing proteins kinase that regulates development and metabolism in every eukaryotic cells. (expandingmarketing.com)
  • Transcription of ribosomal RNA by RNA polymerase (Pol) I initiates ribosome biogenesis and regulates eukaryotic cell growth. (jijitang.com)
  • Regulates non-cell-autonomously the layer formation of the olfactory bulb development and the interneurons. (uniprot.org)
  • An important open question is whether the neurons are dedifferentiated first as a discrete step back to a neural stem cell or even more primitive stem cell state, which then acts like a cancer stem cell or if partial dedifferentiation is enough to kick start the tumorigenic process. (ipscell.com)
  • By combining pulse-chase lineage tracing and single-cell RNA sequencing, we observed that mature dermal adipocytes undergo dedifferentiation and redifferentiation under physiological and pathophysiological conditions. (jci.org)
  • Using the functional human β cell line EndoC-βH1, we demonstrate that polyinosinic-polycytidylic acid (PolyI:C), a synthetic double-stranded RNA that mimics a byproduct of viral replication, induces a decrease in β cell-specific gene expression. (jci.org)
  • This concept is termed dedifferentiation and has been proposed, besides cell death, as a contributing factor to the loss of functional beta-cell mass in diabetes. (garvan.org.au)
  • Patients with type 1 or type 2 diabetes have an insufficiency in their functional β cell mass. (jci.org)
  • Transferring these rodent data to humans has long been challenging, at least partly for technical reasons: primary human islet preparations were scarce and functional human β cell lines were lacking. (jci.org)
  • In 2011, we described a robust protocol of targeted oncogenesis in human fetal pancreas and produced the first functional human β cell line, and in subsequent years additional lines with specific traits. (jci.org)
  • Next, we discuss the use of such functional human β cell lines and share some perspectives on their use to advance diabetes research. (jci.org)
  • In this study, we used functional MRI (fMRI) to investigate age-related neural dedifferentiation in regions of the core and extended face network ( Ishai, 2008 ). (jneurosci.org)
  • Persistent NY-ESO-1 c259 cells were largely functional central memory and stem cell memory T cells. (aacrjournals.org)
  • Has previously established surface marker and functional assays of endothelial progenitor cells in patients with type 1 diabetes and those with thyroid disease. (ncl.ac.uk)
  • If sufficient cells were present in the culture inoculate, a continuous monolayer formed at about 9 days of culture and redifferentiation of smooth muscle began. (biologists.org)
  • In DMEM + 10% FBS, spontaneous redifferentiation of cells was observed for 2 and 3 passages. (radiotec.ru)
  • β cell-specific Polycomb (Eed/PRC2) loss of function in mice triggers diabetes-mimicking transcriptional signatures and highly penetrant, hyperglycemia-independent dedifferentiation, indicating that PRC2 dysregulation contributes to disease. (nih.gov)
  • Implantation of these human bi-potential CD34 + progenitors into immune-deficient NOD-SCID mice resulted in the formation of micro-vessels derived from human endothelial cells that were perfused with mouse and human erythrocytes. (ahajournals.org)
  • Murine CD19 CAR treatment of ALL-bearing mice resulted in long-term remission with long persistence of T cells. (nih.gov)
  • Authors determined that beta-cell-specific loss-of-function of the Polycomb group repressive complex-2 (PRC2) gene expression in mice triggers diabetes-mimicking transcriptional chromatin-state signatures - combined with highly penetrant (in medical genetics, the proportion of those individuals carrying the mutant allele - who actually exhibit clinical symptoms) hyperglycemia (high blood sugar)-independent dedifferentiation. (genewhisperer.com)
  • To enhance the knowledge of β-cell dedifferentiation (BCD) and its causal role in Type 2 diabetes (T2D), my studies employed β-cell line mouse insulinoma 6 (MIN6) and db/db islets from T2D model mice thereby establishing those as models of dedifferentiation. (edu.au)
  • Vitamin D receptor (VDR)-targeted therapy that employed LCA propionate was utilized to treat early passage MIN6 and db /+ mice islets in the pre-diabetic state to protect β-cell from undergoing dedifferentiation. (edu.au)
  • Recently an alternative resistance mechanism has been observed in mice: inflammation-induced dedifferentiation of tumor cells to precursor cells of neural crest origin results in loss of the tumor antigen. (aacrjournals.org)
  • cells of diabetic mice. (biomedsearch.com)
  • Following ablation by the-cell toxin, streptozotocin, gut Ins + cells regenerate and produce insulin, reversing hyperglycemia in mice. (nbdiabetes.org)
  • the reprogramming ESC is called induced pluripotency stem cell (iPSC) [ 5 ]. (ijbs.com)
  • Here, we show that the TFs capable of inducing cell type-specific transcrip-tional profiles prevent the dedifferentiation induced by TFs for pluripotency. (elsevier.com)
  • Nuclear reprogramming to pluripotency can revert both the age and the identity of any cell to that of an embryonic cell. (stanford.edu)
  • Moreover, these cells seem to arise due to the deregulated control of core pluripotency factors and they have been linked to chemotherapy resistance in sarcomas. (mdpi.com)
  • Our new special issue is packed with articles that use mathematical and physical approaches to gain insights into cell and tissue patterning, morphogenesis and dynamics, and that provide a physical framework to capture these processes operating across scales. (biologists.org)
  • The primary reason is that the key mediators of insulin signaling either are poor drug target candidates due to their structure or are shared in common with processes that regulate cell growth. (diabetesjournals.org)
  • We study the processes that control cell-specific gene expression in both embryonic stem cells and in T lymphocytes. (sanger.ac.uk)
  • It is clear that differences between cells within seemingly homogenous populations can be crucial in many biological processes. (sanger.ac.uk)
  • These results suggest that age-related dedifferentiation reflects distinct processes in different brain areas. (jneurosci.org)
  • Neural dedifferentiation could stem from reduced specificity. (jneurosci.org)
  • This approach allowed us to investigate the complete adult life course of neural dedifferentiation. (jneurosci.org)
  • Sorted CD34 + cells were transdifferentiated into induced endothelial cells (iECs) and induced erythroblasts (iErythroblasts) using lineage specific growth factors. (ahajournals.org)
  • Endothelial lineage conversion was dependent on the upregulation of the developmental transcription factor SOX17 whereas suppression of SOX17 instead directed the cells towards an erythroid fate. (ahajournals.org)
  • RESULTS The earliest changes are characterised by loss of surface lining endothelial cells and insudation of blood corpuscular elements admixed with fibrin-platelet thrombus. (bmj.com)
  • In the process, we have revealed novel developmental roles of vascular endothelial cells during heart and craniofacial development. (weizmann.ac.il)
  • Single-cell transcriptomics identifies CD44 as a marker and regulator of endothelial to haematopoietic transition. (sanger.ac.uk)
  • The data demonstrate that FoxO inhibition in endothelial cells has the potential to mediate wide-ranging therapeutic benefits for diabetes-associated cardiovascular disease. (nbdiabetes.org)
  • The scaffold is seeded with isolated myofibroblasts and/or endothelial cells prior to implantation into a recipient mammal. (google.com)
  • 13 . The method of claim 11 , wherein said myofibroblast is cultured in endothelial cell-conditioned media. (google.com)
  • 14 . The method of claim 11 , wherein said myofibrobast is cultured in the presence of an isolated endothelial cell. (google.com)
  • 17 . The method of claim 15 , wherein said myofibrobast is cultured in the presence of an isolated endothelial cell. (google.com)
  • 18 . The method of claim 15 , wherein said myofibroblast is cultured in the presence of a purified endothelial cell-derived growth factor, wherein said growth factor inhibits apoptosis of said myofibroblast. (google.com)
  • In this study, we showed that expression of Oct4 gene or transmembrane delivery of Oct4 protein promoted dedifferentiation of melanoma cells to CSC-like cells. (aacrjournals.org)
  • The dedifferentiated melanoma cells displayed dramatic morphological changes and acquired the ability to form tumor spheroids. (aacrjournals.org)
  • Oct4 induced dedifferentiation was associated with endogenous Oct4 promoter activity, expression of Nanog and Klf4, and global gene expression changes that enriched for transcription factors. (aacrjournals.org)
  • Gene expression variability across cells and species shapes innate immunity. (sanger.ac.uk)
  • Laser capture microdissection LCM was used to extract beta-cell RNA from 1 intact pancreatic islets, 2 freshly isolated islets, 3 islets cultured for 3 days, and changes in gene expression were examined by microarray analysis. (duhnnae.com)
  • Background: Changes in gene expression in pancreatic beta-cells from type 2 diabetes (T2D) should provide insights into their abnormal insulin secretion and turnover. (harvard.edu)
  • Recently, a series of studies were undertaken to re-evaluate the role of oxidative mitochondrial metabolism in cancer cell growth and progression. (nih.gov)
  • CONCLUSIONS β-Cell failure is central to the development and progression of T2D. (diabetesjournals.org)
  • As a result, differentiated cells may also promote relapse and malignant progression in glioma. (oncotarget.com)
  • Mitochondria and cancer: a growing role in apoptosis, cancer cell metabolism and dedifferentiation. (nih.gov)
  • Over time, however, many studies have shown that mitochondria play a fundamental role in cell death by apoptosis or necrosis. (nih.gov)
  • Until recently, the pathophysiology of type 2 diabetes was believed to be characterized by progressive, irreversible loss of pancreatic insulin secretion ( 1 ) mediated by apoptosis of pancreatic β-cells ( 2 ). (diabetesjournals.org)
  • Thus, bFGF induces oligodendroglial dedifferentiation if oligodendroglial DNA synthesis can occur but causes oligodendoglial apoptosis when oligodendroglial DNA synthesis is prevented. (elsevier.com)
  • Background: There is great interest about the possible contribution of ER stress to the apoptosis of pancreatic beta cells in the diabetic state and with islet transplantation. (harvard.edu)
  • With time distinct neointimal thickening occurs, associated with disappearance of proliferative activity and a phenotypic shift of the smooth muscle cells. (bmj.com)
  • These alterations lead to phenotypic reconfiguration of beta-cells and ultimately defective insulin secretion. (garvan.org.au)
  • Heightened interest in applicability of NK cells for cancer immunotherapy has been evoked by improved methodologies for their purification, molecular and phenotypic characterization. (frontiersin.org)
  • To address these questions, MSC were differentiated into adipogenic lineage cells, followed by dedifferentiation. (eurekamag.com)
  • Subsequent lineage tracing demonstrated that the luminal secretory cells had dedifferentiated into basal stem cells. (jijitang.com)
  • In addition, the TFs that interfered with dedifferentiation in hepatic-lineage cells involved TFs with known induction activity for hepatic-lineage cells. (elsevier.com)
  • Beta-cells are now known to be highly adaptable, and pioneering studies over the last decade have discovered networks of transcriptional and chromatin regulators that drive the development of beta-cell lineage. (genewhisperer.com)
  • Lineage tracing experiments show that gut Ins + cells arise cell-autonomously from Foxo1-deficient cells. (nbdiabetes.org)
  • The aim of this study was to determine if treatment with reversine, a purine analog, promoted generation of skeletal progenitor cells from lineage-committed annulus fibrosus cells. (bjmu.edu.cn)
  • You have to show something biochemically (immunostaining, PCR ,western) to show that the cell shuttles between two differentiated adult kind to prove dedifferentiation. (protocol-online.org)
  • That dogma has been undermined by the recognition that the adult mammalian heart contains its own reservoir of progenitor (or stem) cells [1] - [5] . (pubmedcentralcanada.ca)
  • Each of the approximately 37.2 trillion (3.72x1013) cells in an adult human has its own copy or copies of the genome except certain cell types, such as red blood cells, that lack nuclei in their fully differentiated state. (wikipedia.org)
  • germline stem cells lacking Jak-STAT signaling differentiate into spermatogonia without self-renewal. (sciencemag.org)
  • Numerous studies have shown the importance of cancer stem cells in cancer resistance and self-renewal. (aacrjournals.org)
  • NADPH-oxidase (NOX)-dependent reactive oxygen species (ROS) production is involved in self-renewal of stem and progenitor cells. (unboundmedicine.com)
  • Herein, we investigated whether high glucose (25 mM/L) (HG)-dependent NOX-mediated ROS generation is involved in self-renewal of visceral adipose tissue-derived stem cells (ASCs) as well. (unboundmedicine.com)
  • Finally, unipotent cells can differentiate into only one cell type, but are capable of self-renewal. (wikipedia.org)
  • Myosins, actin-dependent molecular motors, are likely to contribute to tumor formation and metastasis via their effects on cell adhesion and migration and may provide promising new targets for cancer therapies. (oncotarget.com)
  • However, information about molecular events during dedifferentiation is not well elucidated. (molvis.org)
  • She uses the fruit fly testis as a model system, studying the molecular signals that control whether germ line stem cells form more stem cells or become sperm. (hopkinsmedicine.org)
  • Current study favors that transdifferentiation is dedifferentiation dependent process. (eurekamag.com)
  • It can be induced by nuclear transfer, cell fusion, or forced transcription factors expression [ 1 , 2 ]. (ijbs.com)
  • Many dedifferentiated cardiomyocytes went on to express the stem cell antigen c-kit, and the early cardiac transcription factors GATA4 and Nkx2.5 . (pubmedcentralcanada.ca)
  • These factors are regulated by key transcription factors that are known to control cancer stem cells and dedifferentiation. (aacrjournals.org)
  • These approaches lose dynamic information from individual cells, and give the impression transcription is a continuous smooth process. (ucl.ac.uk)
  • We are testing the implications of noisy transcription on the generation of diversity between cells during development and for improving regenerative medicine. (ucl.ac.uk)
  • Inflammation-induced tumor cell dedifferentiation resulted in loss of the tumor antigen and relapse. (aacrjournals.org)
  • We report that cell fate determination is reversible process. (eurekamag.com)
  • Critically, the β-cell dedifferentiation produced by long-term excess nutrient supply is reversible. (diabetesjournals.org)
  • This review summarizes the evidence that early type 2 diabetes can be regarded as a reversible β-cell response to chronic positive calorie balance. (diabetesjournals.org)
  • Human chondrocytes, epidermal cells, pancreatic beta cells and adipose stromal cells dedifferentiate and exhibit stem cell phenotypes [11] - [15] . (pubmedcentralcanada.ca)
  • This is groundbreaking because these types of cells, especially the mature neurons, were thought to be really fixed in their fate and hence possessing much plasticity. (ipscell.com)
  • Here, we combine deep epigenome mapping with single-cell transcriptomics to mine for evidence of chromatin dysregulation in type 2 diabetes. (nih.gov)
  • Importantly, the data suggest a two-hit (chromatin and hyperglycemia) model for loss of β cell identity in diabetes. (nih.gov)
  • Fluorescence in situ hybridization analysis of interphase nuclei showed high levels of rDNA chromatin condensation in both callus and leaf, with substantially less decondensed rDNA than is observed in meristematic root-tip cells. (plantphysiol.org)
  • Authors [see attached article] combined deep epigenome-wide mapping analysis with single-cell transcriptomics to search for evidence of chromatin dysregulation in T2D. (genewhisperer.com)
  • Most fundamentally - this study suggests a "two-hit model" (i.e. chromatin, plus hyperglycemia) for explaining the loss of beta-cell identity in diabetes [and these data likely apply to both the autoimmune type-1 diabetes, as well as type-2 diabetes]. (genewhisperer.com)
  • Furthermore, ShRNA mediated knockdown of Oct4 in dedifferentiated cells led to diminished CSC phenotypes. (aacrjournals.org)
  • In this article, we review the literature and highlight how the TME manipulates the NK cell phenotypes, genotypes, and tropism to evade tumor recognition and elimination. (frontiersin.org)
  • Furthermore, human iPS cells and other stem cells may provide various types of cells for regenerative medicine. (keystonesymposia.org)
  • But recent research has shown that β-cells do not die in diabetes, but undergo a silencing process, termed "dedifferentiation. (nih.gov)
  • Type 1 diabetes (T1D) is a chronic disease characterized by an autoimmune-mediated destruction of insulin-producing pancreatic β cells. (jci.org)
  • In chapter I of this dissertation, we isolated and functionally characterized failing β-cells from various experimental models of diabetes. (columbia.edu)
  • Ultimately, diabetes results from insufficient numbers of insulin-secreting beta-cells - caused by impaired function, increased cell death, and/or loss of cell identity. (genewhisperer.com)
  • In people with type 1 diabetes, β cells are selectively destroyed by an autoimmune attack, creating an absolute insulin deficiency. (jci.org)
  • In type 1 diabetes (T1D) β cell mass is markedly reduced by autoimmunity. (harvard.edu)
  • Type 2 diabetes (T2D) results from inadequate β cell mass and function that can no longer compensate for insulin resistance. (harvard.edu)
  • Stem cells hold great promise for pancreatic beta cell replacement therapy for diabetes. (harvard.edu)
  • Role of p53 in the cross-talk between cancer cells and their microenvironment. (weizmann.ac.il)
  • Effect of Genotype, Explant Type and 2,4-D on Cell Dedifferentiation and Callus Induction in Cumin ( Cuminum cyminum L.) Medicinal Plant', Journal of Applied Biotechnology Reports , 2(3), pp. 265-270. (biotechrep.ir)
  • In osteogenic media, cells pretreated with 300nM reversine exhibited an increased induction in alkaline phosphatase activity and enhanced expression of alkaline phosphatase, bone sialoprotein, osteocalcin, and collagen type I mRNA. (bjmu.edu.cn)
  • In chondrogenic media, cells pretreated with reversine exhibited marked increase in the induction of aggrecan, collagen types II, IX, and XI, and versican. (bjmu.edu.cn)
  • In postamputation newts, epidermal cells cover the wound to form a blastema. (mun.ca)
  • CD19 has been targeted through genetically engineered chimeric-antigen receptor (CAR) T cells, and via bispecific CD3-CD19 antibodies, with reports of relapse via CD19 loss. (nih.gov)
  • The cells were treated with antibodies to membrane surface protein integrin β1 (red channel) ( A1-D1 ). (springeropen.com)
  • Plants, like animals, regenerate cells and tissues after damage such as wounds and injury have occurred. (wikipedia.org)
  • Heavily irradiated Hydra, that cannot undergo cell division (grow) will regenerate. (mun.ca)
  • Recent data suggest that viral infection of human islets leads to a decrease in insulin production rather than β cell death, suggesting loss of β cell identity. (jci.org)
  • In addition, various stem cells have been isolated from human tissues. (keystonesymposia.org)
  • A cell atlas of human thymic development defines T cell repertoire formation. (sanger.ac.uk)
  • Sarah Teichmann is co-founder and principal leader of the Human Cell Atlas (HCA) international consortium. (sanger.ac.uk)
  • The International Human Cell Atlas initiative aims to create comprehensive reference maps of all human cells to further understand health and disease. (sanger.ac.uk)
  • She is co-founder of the global Human Cell Atlas Consortium. (sanger.ac.uk)
  • The International Human Cell Atlas initiative aims to create comprehensive reference maps of all human cells-the fundamental units of life-as a basis for both understanding human health and diagnosing, monitoring, and treating disease. (sanger.ac.uk)
  • However, the biological response of human islet cells to isolation remains poorly characterized. (duhnnae.com)
  • Harvard Stem Cell Scientists have discovered that the same chemicals that stimulate muscle development in zebrafish can also be used to differentiate human stem cells into muscle cells in the laboratory, an historically challenging task that, now overcome, makes muscle cell therapy a more realistic clinical possibility. (constantcontact.com)
  • In this Review, we first explain how we developed the human β cell lines and why we think we succeeded where others, despite major efforts, did not. (jci.org)
  • An increase in human islet preparations available for research has improved our understanding of what a human β cell is, but the material remains scarce, its availability is unpredictable, and the quality is variable. (jci.org)
  • The cells of the inner cell mass go on to form virtually all of the tissues of the human body. (wikipedia.org)
  • Although the cells of the inner cell mass can form virtually every type of cell found in the human body, they cannot form an organism. (wikipedia.org)
  • Transient non-integrative expression of nuclear reprogramming factors promotes multifaceted amelioration of aging in human cells. (stanford.edu)
  • In normal β-cells, mitochondria generate energy required to sustain insulin production and its finely timed release in response to the body's nutritional status. (nih.gov)
  • Autophagy is a physiological stress response, in which cytoplasmic contents including mitochondria are recycled to aid cell survival in response to nutrient starvation. (bl.uk)
  • [1] Muller glia are a type of glial cell present in both the teleost and mammalian retina. (wikipedia.org)
  • In this study, we show that Myc overexpression is required for proper dedifferentiation of radial glial cells and for triggering the programmed cell death in the vicinity of the injury. (biomedcentral.com)
  • Among the most prominent components of the early post-injury response are a sharp increase in programmed cell death and extensive dedifferentiation of glial cells in the vicinity of the lesion. (biomedcentral.com)
  • The dedifferentiated glial cells become highly proliferative and act as a major source of both new glial cells and new neurons in the regenerating segment of the radial nerve cord [ 1 , 3 ]. (biomedcentral.com)
  • The therapeutic potential of the generated cells was assessed in an experimental model of myocardial infarction. (ahajournals.org)
  • We combine novel approaches to study cardiomyocyte (CM) renewal by promoting CM cell division and dedifferentiation, as a potential therapeutic strategy to cure the injured heart. (weizmann.ac.il)
  • Topics covered include genetic predisposition, foundations of β-cell failure, natural history of β-cell failure, and impact of therapeutic interventions. (diabetesjournals.org)
  • Due to the mode of NK cell killing, requiring one-to-one target engagement and site-directed release of cytolytic granules, the therapeutic potential of NK cells has been most extensively explored in hematological malignancies. (frontiersin.org)
  • However, their ability to precisely kill antibody coated cells, cancer stem cells, and genotoxically altered cells, while maintaining tolerance to healthy cells makes them appealing therapeutic effectors for all cancer forms, including metastases. (frontiersin.org)
  • Flow-sorted Aldh1a3-expressing (ALDH+) islet cells demonstrate impaired glucose-induced insulin secretion, are depleted of Foxo1 and MafA, and include a Neurogenin3-positive subset. (columbia.edu)
  • Among dividing cells, there are multiple levels of cell potency, the cell's ability to differentiate into other cell types. (wikipedia.org)
  • A cell that can differentiate into all cell types, including the placental tissue, is known as totipotent. (wikipedia.org)
  • A multipotent cell is one that can differentiate into multiple different, but closely related cell types. (wikipedia.org)
  • Oligopotent cells are more restricted than multipotent, but can still differentiate into a few closely related cell types. (wikipedia.org)
  • Cells differentiate to specialize for different functions. (wikipedia.org)
  • Her research focuses on how stem cells renew and differentiate. (hopkinsmedicine.org)
  • Protein localization was determined by immunofluorescent staining on corneoscleral tissue cryosections and in cell cultures. (arvojournals.org)
  • Over weeks, these cells become cartilage, muscle and connective tissue. (mun.ca)
  • Reversine Enhances Generation of Progenitor-like Cells by Dedifferentiation of Annulus Fibrosus Cells[J]. TISSUE ENGINEERING PART A,2010,16(4):1443-1455. (bjmu.edu.cn)
  • The term undifferentiated is used to describe a cell (or a tissue) that has not yet acquired a special structure and function. (biology-online.org)