The simultaneous identification of all chromosomes from a cell by fluorescence in situ hybridization (IN SITU HYBRIDIZATION, FLUORESCENCE) with chromosome-specific florescent probes that are discerned by their different emission spectra.
Mapping of the KARYOTYPE of a cell.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Abnormal number or structure of chromosomes. Chromosome aberrations may result in CHROMOSOME DISORDERS.
A technique for visualizing CHROMOSOME ABERRATIONS using fluorescently labeled DNA probes which are hybridized to chromosomal DNA. Multiple fluorochromes may be attached to the probes. Upon hybridization, this produces a multicolored, or painted, effect with a unique color at each site of hybridization. This technique may also be used to identify cross-species homology by labeling probes from one species for hybridization with chromosomes from another species.
Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping.
Neoplasms composed of fatty tissue or connective tissue made up of fat cells in a meshwork of areolar tissue. The concept does not refer to neoplasms located in adipose tissue.
A type of chromosome aberration characterized by CHROMOSOME BREAKAGE and transfer of the broken-off portion to another location, often to a different chromosome.
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
A specific pair of GROUP D CHROMOSOMES of the human chromosome classification.
The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. In a normally diploid cell (DIPLOIDY) the loss of a chromosome pair is termed nullisomy (symbol: 2N-2), the loss of a single chromosome is MONOSOMY (symbol: 2N-1), the addition of a chromosome pair is tetrasomy (symbol: 2N+2), the addition of a single chromosome is TRISOMY (symbol: 2N+1).
A type of chromosomal aberration involving DNA BREAKS. Chromosome breakage can result in CHROMOSOMAL TRANSLOCATION; CHROMOSOME INVERSION; or SEQUENCE DELETION.
The ordered rearrangement of gene regions by DNA recombination such as that which occurs normally during development.
Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Regulatory signaling systems that control the progression through the CELL CYCLE. They ensure that the cell has completed, in the correct order and without mistakes, all the processes required to replicate the GENOME and CYTOPLASM, and divide them equally between two daughter cells. If cells sense they have not completed these processes or that the environment does not have the nutrients and growth hormones in place to proceed, then the cells are restrained (or "arrested") until the processes are completed and growth conditions are suitable.
The period of the CELL CYCLE preceding DNA REPLICATION in S PHASE. Subphases of G1 include "competence" (to respond to growth factors), G1a (entry into G1), G1b (progression), and G1c (assembly). Progression through the G1 subphases is effected by limiting growth factors, nutrients, or inhibitors.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A cell line derived from cultured tumor cells.
The period of the CELL CYCLE following DNA synthesis (S PHASE) and preceding M PHASE (cell division phase). The CHROMOSOMES are tetraploid in this point.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Phase of the CELL CYCLE following G1 and preceding G2 when the entire DNA content of the nucleus is replicated. It is achieved by bidirectional replication at multiple sites along each chromosome.
CELL CYCLE regulatory signaling systems that are triggered by DNA DAMAGE or lack of nutrients during G2 PHASE. When triggered they restrain cells transitioning from G2 phase to M PHASE.
A quiescent state of cells during G1 PHASE.
A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A large family of regulatory proteins that function as accessory subunits to a variety of CYCLIN-DEPENDENT KINASES. They generally function as ENZYME ACTIVATORS that drive the CELL CYCLE through transitions between phases. A subset of cyclins may also function as transcriptional regulators.
A cyclin-dependent kinase inhibitor that mediates TUMOR SUPPRESSOR PROTEIN P53-dependent CELL CYCLE arrest. p21 interacts with a range of CYCLIN-DEPENDENT KINASES and associates with PROLIFERATING CELL NUCLEAR ANTIGEN and CASPASE 3.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
Regulatory signaling systems that control the progression of the CELL CYCLE through the G1 PHASE and allow transition to S PHASE when the cells are ready to undergo DNA REPLICATION. DNA DAMAGE, or the deficiencies in specific cellular components or nutrients may cause the cells to halt before progressing through G1 phase.
Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.
A cyclin-dependent kinase inhibitor that coordinates the activation of CYCLIN and CYCLIN-DEPENDENT KINASES during the CELL CYCLE. It interacts with active CYCLIN D complexed to CYCLIN-DEPENDENT KINASE 4 in proliferating cells, while in arrested cells it binds and inhibits CYCLIN E complexed to CYCLIN-DEPENDENT KINASE 2.
Clinical conditions caused by an abnormal chromosome constitution in which there is extra or missing chromosome material (either a whole chromosome or a chromosome segment). (from Thompson et al., Genetics in Medicine, 5th ed, p429)
The full set of CHROMOSOMES presented as a systematized array of METAPHASE chromosomes from a photomicrograph of a single CELL NUCLEUS arranged in pairs in descending order of size and according to the position of the CENTROMERE. (From Stedman, 25th ed)
Tumors or cancer of the MOUTH.
An increased tendency to acquire CHROMOSOME ABERRATIONS when various processes involved in chromosome replication, repair, or segregation are dysfunctional.
The phase of cell nucleus division following METAPHASE, in which the CHROMATIDS separate and migrate to opposite poles of the spindle.
The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.
Large multiprotein complexes that bind the centromeres of the chromosomes to the microtubules of the mitotic spindle during metaphase in the cell cycle.
The phase of cell nucleus division following PROMETAPHASE, in which the CHROMOSOMES line up across the equatorial plane of the SPINDLE APPARATUS prior to separation.
A microtubule structure that forms during CELL DIVISION. It consists of two SPINDLE POLES, and sets of MICROTUBULES that may include the astral microtubules, the polar microtubules, and the kinetochore microtubules.
INFLAMMATION of the LIVER in humans caused by HEPATITIS C VIRUS, a single-stranded RNA virus. Its incubation period is 30-90 days. Hepatitis C is transmitted primarily by contaminated blood parenterally, and is often associated with transfusion and intravenous drug abuse. However, in a significant number of cases, the source of hepatitis C infection is unknown.
A condition characterized by the presence of abnormal quantities of CRYOGLOBULINS in the blood. Upon cold exposure, these abnormal proteins precipitate into the microvasculature leading to restricted blood flow in the exposed areas.
A genus of FLAVIVIRIDAE causing parenterally-transmitted HEPATITIS C which is associated with transfusions and drug abuse. Hepatitis C virus is the type species.
INFLAMMATION of the LIVER in humans that is caused by HEPATITIS C VIRUS lasting six months or more. Chronic hepatitis C can lead to LIVER CIRRHOSIS.
Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of signal transduction and gene expression, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS.
Proteins encoded by a VIRAL GENOME that are produced in the organisms they infect, but not packaged into the VIRUS PARTICLES. Some of these proteins may play roles within the infected cell during VIRUS REPLICATION or act in regulation of virus replication or VIRUS ASSEMBLY.
Nitrogenous products of NITRIC OXIDE synthases, ranging from NITRIC OXIDE to NITRATES. These reactive nitrogen intermediates also include the inorganic PEROXYNITROUS ACID and the organic S-NITROSOTHIOLS.
Tumors or cancer of the STOMACH.
Rapid methods of measuring the effects of an agent in a biological or chemical assay. The assay usually involves some form of automation or a way to conduct multiple assays at the same time using sample arrays.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
Large collections of small molecules (molecular weight about 600 or less), of similar or diverse nature which are used for high-throughput screening analysis of the gene function, protein interaction, cellular processing, biochemical pathways, or other chemical interactions.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
A serine threonine kinase that controls a wide range of growth-related cellular processes. The protein is referred to as the target of RAPAMYCIN due to the discovery that SIROLIMUS (commonly known as rapamycin) forms an inhibitory complex with TACROLIMUS BINDING PROTEIN 1A that blocks the action of its enzymatic activity.
The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells.
Spectral karyotype[edit]. Spectral karyotyping is an image of colored chromosomes. Spectral karyotyping involves FISH using ... which require dividing cells and requires labor and time-intensive manual preparation and analysis of the slides by a ... and miRNA in tissues and cells. FISH is used by examining the cellular reproduction cycle, specifically interphase of the ... FISH on sperm cells is indicated for men with an abnormal somatic or meiotic karyotype as well as those with oligozoospermia, ...
... cell cycle analysis and "flow karyotyping" of cells. In flow karotyping, isolated metaphase chromosomes are stained and ... Additionally, if the dye undergoes a spectral shift then you can determine the absolute concentration of the target regardless ... Flow microfluorimetry is also used in pharmaceutical research to determine cell type, protein and DNA expression, cell cycle, ... Microfluorimetry expands on fluorimetry by adding a microscopic component to measurements to allow analysis of single cells and ...
interphase All stages of the cell cycle excluding cell division. A typical cell spends most of its life in interphase, during ... spectral karyotype (SKY) spliceosome splicing See genetic engineering. split-gene standard genetic code The genetic code used ... The result of such analyses is known as a phylogeny or phylogenetic tree. plasmid Any small DNA molecule that is physically ... any cell other than a gamete, germ cell, or undifferentiated stem cell. Somatic cells are theoretically distinct from cells of ...
... and it can enhance cell cycle progression, cell survival, and block normal cell death (apoptosis). It has been demonstrated ... Molecular cytogenetic characterization by use of spectral karyotyping and comparative genomic hybridization". International ... An analysis of fifty-one cases". The Journal of Bone and Joint Surgery. American Volume. 70 (6): 862-70. doi:10.2106/00004623- ... Cancer stem cells (or cancer-initiating cells) are thought to be a small population of cells within the tumor that are directly ...
"Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping". Cancer Res. 59 (1 ... Duesberg, P; Mandrioli, D; McCormack, A; Nicholson, JM (2011). "Is carcinogenesis a form of speciation?". Cell Cycle. ... "Comparative analysis and integrative classification of NCI60 cell lines and primary tumors using gene expression profiling data ... These cells proliferate abnormally rapidly, even compared to other cancer cells. Like many other cancer cells, HeLa cells have ...
Edgar BA, Orr-Weaver TL (May 2001). "Endoreplication cell cycles: more for less". Cell. 105 (3): 297-306. doi:10.1016/S0092- ... July 1996). "Multicolor spectral karyotyping of human chromosomes". Science. 273 (5274): 494-7. Bibcode:1996Sci...273..494S. ... Thus, chromosomes or chromosome sections can be visualized and identified, allowing for the analysis of chromosomal ... Polyploid cells have multiple copies of chromosomes and haploid cells have single copies. Karyotypes can be used for many ...
"Endoreplication cell cycles: more for less". Cell. 105 (3): 297-306. doi:10.1016/S0092-8674(01)00334-8. PMID 11348589.. ... Multicolor FISH and the older spectral karyotyping are molecular cytogenetic techniques used to simultaneously visualize all ... Thus, chromosomes or chromosome sections can be visualized and identified, allowing for the analysis of chromosomal ... A karyotype is the number and appearance of chromosomes in the nucleus of a eukaryotic cell. The term is also used for the ...
... cytogenetic analysis and spectral karyotyping to investigate the chromosomal segregation defects in cultured oral squamous cell ... or from misregulation of the cell cycle, for example, uncoupling DNA replication from cell division (3) or centrosomal ... oral squamous cell carcinomas;. DAPI,. 4′,6-diamidino-2-phenylindole;. SKY,. spectral karyotyping;. MTOC,. microtubule ... Spectral Karyotyping (SKY).. Prior to SKY, the metaphase cells were G-banded as above. Within 24 hr, the slides were destained ...
The cell line was cytogenetically characterized using array CGH, spectral karyotyping (SKY) and fluorescence in situ ... We have used a cell line, CAL 33, for the establishment of a cell culture model in order to perform functional analyses of ... Radiosensitivity of CAL 33 cells was intermediate when compared to published data on tumor cell lines. ... Adequate cell culture model systems are required for functional studies investigating those potential prognostic markers in ...
Karyotype analysis. Metaphases were prepared from methanol/acetic acid (3:1)-fixed cells. Slides were hybridized by spectral ... 2A). Cell cycle analysis showed an increase in the percentage of S-phase cells in postcrisis cultures, with reduced duplication ... 2A). Cell cycle analysis showed generalized chromosome instability, as described (ref. 8; Supplementary Fig. S1A). Of the ... Fluorescence-activated cell sorting analysis. Cells were analyzed in an EPICS XL-MCL cytometer (Coulter Electronics, Hialeah, ...
However, recent analyses with techniques such as comparative genomic hybridization and spectral karyotyping (which possess ... excess activity of MYC forces cells into the cell cycle under deleterious conditions such as those imposed by treating cells ... Cells were treated with E2 (10 μM) for two days, followed by analysis of metaphase spreads (15). Untreated Rat1A(MYCER) cells ... Excess MYC activity causes inappropriate cell cycle entry. NHF(BABE) or NHF(MYCER) cells were treated with PALA (50 μM) for two ...
Spectral karyotyping (SKY) analysis of lymphoma cells from PTEN-ΔT mice revealed a 100% incidence of a reciprocal 14;15 ... At various time points after treatment onset, the effects of GSI on cell growth, cell viability, and cell cycle distribution ... Cell cycle analyses showed that cell fractions in G2/S/M phase were diminished, and fractions in sub-G0/G1 phase were markedly ... cell cycle arrest, and apoptosis in t(14;15)-negative tumor cell lines treated with GSI. (F) Western blot analysis of the ...
... which eventually produces cell cycle-arrested cells with complex karyotypes. This paper... ... as shown by representative karyotype analysis from single-cell sequencing in Figure 3, in which ArCK cells display multiple, ... Spectral Karyotyping to Study Chromosome Abnormalities in Humans and Mice with Polycystic Kidney Disease… ... cells remaining on the plate are cell cycle arrested and, as shown recently10, harbor complex karyotypes. These cells are ...
... analysis under the FL-2H detector using FACS scan. To determine the cell cycle distribution of the stable cell lines, we ... Spectral karyotyping. Cytogenetic characterization using spectral karyotyping (SKY) was carried out as previously described ( ... stable cell lines resulting from the disrupted cell cycle control. The AIMP3+/− stable cells showed uneven cell division at ... Next, we checked the possible abnormality in cell cycle control using chemical inhibitors of cell cycle progression. Although ...
Karyotype analysis. Metaphase chromosomes were prepared using standard procedures (4). Cells were partially synchronized by ... Spectral karyotyping. Multicolor spectral karyotyping (SKY) was done, as previously described (4). In situ hybridization was ... and impair cell-cycle regulation in stably transformed Chinese hamster ovary cells (27). The core protein also induces dsDNA ... FLAG-tagged Nbs1 constructs were transfected into Huh7 cells, and cells were collected for analysis 36 h later. For immunoblots ...
Conventional cytogenetic and spectral karyotyping analysis of mitotically active TLBR-2 and -3 cells showed clonally abnormal, ... Dysregulation of cell-cycle and apoptosis controls. Aberrant expression of cell-cycle control genes and escape from homeostatic ... dendritic cell (DC; CD1a−), and stem cell (c-kit−CD133−) markers. In regard to normal T-cell lineages, analysis of T-cell ... CD30-positive anaplastic large cell lymphoma cell lines. In: Masters JRW, Palsson BO , editors. Human cell culture: cancer cell ...
Unsupervised hierarchical cluster analysis shows select gene expression alterations in c.1380delA CDH1 SB.mhdgc-1 cells ... 100-fold increased activity in hereditary c.1380delA CDH1 gastric cancer cells inducing apoptosis most effectively in cells ... Differential high-throughput drug screening of c.1380delA CDH1 SB.mhdgc-1 versus sporadic gastric cancer cells identified ... Integrated pharmacological and transcriptomic profiling of hereditary diffuse gastric cancer cells with a loss-of-function c. ...
High-resolution spectral karyotyping analyses at passage 24-25 did, however, indicate the expected karyotype variability with ... PCR amplification (touchdown PCR protocol: 20 cycles of 95°C for 15 seconds, 60°C-56°C for 15 seconds; 72°C for 20 seconds, and ... Spectral karyotyping (SKY). Cells were prepared as described above and dropped onto glass slides in a climate chamber (Polymer ... 3 dishes per cell line and time point) into single cells in 0.25% trypsin EDTA and counted the total number of cells. Cell- ...
Chromosome fragmentation is a major form of mitotic cell death which is identifiable during common cytogenetic analysis by its ... In this analysis the phenomena of chromosome fragmentation and PCC are reviewed and their similarities and differences are ... Mitotic cell death is an important form of cell death, particularly in cancer. ... as chromosome fragmentation analysis can be used as a relatively quick and inexpensive substitute for spectral karyotyping in ...
Spectral karyotype analysis of colon cancer cell lines of the tumor suppressor and mutator pathway. Cytogenet Genome Res. 2002; ... Waldman TA, Gene Targeting in Cultured Human Cells. Cell Cycle Checkpoint Control Protocols. Ed. HB Lieberman. Totowa, NJ: ... Immortalized Cells. Immortalized cells, on the other hand, which began laboratory life as primary cells, can be converted to ... At the moment, the routine cell line choices for in vitro work are primary cells, tumor-derived cells, or "immortalized" lines ...
Gross Cytology and Cell Counting, Staining; Cell Cycle and Mycoplasma Analysis; Cell Line Authentication; Karyotyping and ... Spectral Unmixing; Live Cell Studies: Translocation and localization of proteins using fluorescent fusion proteins and time ... Bone Marrow Stem Cell Plasticity; Hematopoietic Stem Cells; Cell Lines as Models of as Stem Cells; Flow Cytometry and Stem Cell ... Different Types of Adult Stem Cells; Pluriptotent Stem Cells; Neural Stem Cells; Epigenetics and Stem Cells; Cancer Stem Cells ...
Learn in-depth information on STAT3d Mutation Analysis Test, on why the laboratory test is performed, specimen collected, the ... Stat 3 is known to increase production of a crucial cell cycle protein called cyclin D1. Cyclin D1 increases the rate of growth ... Karyotyping including spectral karyotyping. *mRNA analysis. *Tissue microarrays (TMAs). *Southern blot test ... STAT3D Mutation Analysis Test. What is STAT3d Mutation Analysis Test? (Background Information). *STAT3d mutation refers to an ...
Spectral karyotyping analysis of tumor cells revealed massive changes in chromosomal number and structure. Similar chromosome ... The progression of cells through the cell cycle is precisely regulated by multiple checkpoints at different transition phases ... DNA synthesis analysis. DNA synthesis analysis was assessed as described [33]. Briefly, cells were cultured in the logarithmic ... Cell cycle. 2013;12:2564-9 6. Shiloh Y. ATM and ATR: networking cellular responses to DNA damage. Curr Opin Genet Dev. 2001;11: ...
Cell cycle analysis.For all flow cytometry experiments, both cells growing on the surface of the dishes and in the culture ... A) Spectral karyotype of a metaphase with 142 chromosomes, represented in classification pseudocolors. (B) Spectral karyotype ... 3B). These findings suggest that PRMT1 deficiency causes cell death or cell cycle arrest, and thus the cells that were not ... cell cycle checkpoints are activated to arrest cell cycle progression allowing time for repair. The G1/S checkpoint prevents ...
At last we compared cell cycle and spectral karyotype between two groups.,/p,,p,,b,RESULTS,/b,In SFM consisting of EGF and bFGF ... Cell cycle analysis indicated that tumor spheres were of a high proliferation state.We could not find any noticeable difference ... Cell Shape , Physiology , Cells, Cultured , Hepatic Stellate Cells , Cell Biology , Metabolism , Humans , Phosphorylation , RNA ... AC133 Antigen , Antigens, CD , Metabolism , Cell Culture Techniques , Methods , Cell Line, Tumor , Cell Proliferation , Culture ...
At last we compared cell cycle and spectral karyotype between two groups.,/p,,p,,b,RESULTS,/b,In SFM consisting of EGF and bFGF ... Cell cycle analysis indicated that tumor spheres were of a high proliferation state.We could not find any noticeable difference ... AC133 Antigen , Antigens, CD , Metabolism , Cell Culture Techniques , Methods , Cell Line, Tumor , Cell Proliferation , Culture ... Then we analysed expressions of stem cell surface markers CD133 and CD44 among undifferentiated cell, post-differentiated cells ...
Hedgehog modulates cell cycle regulators in stem cells to control hematopoietic regeneration. Proc Natl Acad Sci U S A 2006;103 ... spectral karyotyping, and comparative genomic hybridization analyses confirmed that the specific biologic property of FLA2 ... A) E14.5 FL cells were isolated from C57BL/6:Pep3b (Ly5.1) mice and sorted for KLS cells. A fraction of sorted cells was plated ... Isolation of murine hematopoietic cell subpopulations. Cells were first purified for Sca1 using magnetic activated cell sorting ...
Cell cycle arrest and therapeutic sensitivity in de novo AML.. D Banker;C Willman;M Groudine;F Appelbaum Blood 90(10)Suppl.1: ... Twenty-four-color spectral karyotyping reveals chromosome aberrations in cytogenetically normal acute myeloid leukemia ... Second Cycle Remission Achievement with 7+3 and Survival in Adults with Newly Diagnosed Acute Myeloid Leukemia: Analysis of ... Deregulation of stem cell factor (SCF)-AKT-S6 pathway in diagnostic AML samples is associated with disease-free survival: ...
C) Karyotype analysis. Spectral karyotyping (SKY) was performed to detect microscopic genomic abnormalities, translocations and ... Článek Cell Cycle Control by the Master Regulator CtrA in Článek Myopathic Lamin Mutations Cause Reductive Stress and Activate ... Analysis of T-cell phenotype and genotype. For flow cytometric analysis, cells were resuspended in FACS buffer [PBS ... Článek Cell Specific eQTL Analysis without Sorting Cells Článek Cooperative Action of Cdk1/cyclin B and SIRT1 Is Required for ...
Both attached and floating cells were harvested for cell cycle analysis. For each experiment, 10,000 cells were analyzed using ... of three novel translocations and specific common chromosomal break sites in malignant melanoma by spectral karyotyping. Genes ... cell lines in apoptosis and cell distribution in G0/G1-, S- and G2/M-phases of the cell cycle before and after UV irradiation [ ... Survival analysis of UACC903(+6) and UACC903 cell lines. (a) Survival curves of UACC903(+6) and UACC903 cells in response to ...
What is the cell cycle in which most chromosome analysis is conducted?. Metaphase. ... Spectral karyotyping (uses 24 different colored fluorescent probes simultaneously). Heteroploid. any chromosome number other ... Which cell types are used in cell culture for FISH analysis?. Leukocytes & Lymphoblastoids (peripheral blood), fibroblasts ( ... in vitro fertilization, single blasttomere tested (8-16 cells), DNA analysis for single disorder, only conducted if parents/ ...
Analysis of cell-cycle phase distribution showed an accumulation of cells in the G0/G1 phase and a concomitant decrease in the ... Spectral karyotyping analysis of these cases deciphered the characteristics of several marker chromosomes and complex ... significantly decreased cell proliferation and cell cycle perturbation associated with upregulation of p21 and p27 cell-cycle ... MicroRNAs (miRNAs) are small noncoding RNAs that actively modulate cell physiological processes as apoptosis, cell-cycle ...
... and centrosome abnormalities were observed in the cyclin D1-rescued cells by spectral karyotyping and immunofluorescence. To ... Further analysis revealed that Cdc42-deficient stem cells had cell division defects, reduced capacity for clonal expansion and ... DYRK2 priming phosphorylation of c-Jun and c-Myc modulates cell cycle progression in human cancer cells. ... DYRK2 priming phosphorylation of c-Jun and c-Myc modulates cell cycle progression in human cancer cells. ...
... thus linking control over homing and migration of T cells, as well cell cycle entry, apoptosis, and DNA damage and oxidative ... thus linking control over homing and migration of T cells, as well cell cycle entry, apoptosis, and DNA damage and oxidative ... Akt acts as a central mediator of PI3K signals, downstream of which is the mTOR pathway, controlling cell growth and metabolism ... Akt acts as a central mediator of PI3K signals, downstream of which is the mTOR pathway, controlling cell growth and metabolism ...
... and spectral karyotyping (SKY)] has been used to determine the karyotype of a CCL2 HeLa cell line (Macville et al. 1999). This ... Our analysis of the HeLa gene expression profile revealed that several pathways, including cell cycle and DNA repair, exhibit ... per cell. The comparison of their spectral karyotype with previously published G-banding karyotypes (Francke et al. 1973; ... 1999 Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping. Cancer Res. 59 ...
We used Spectral Karyotyping (SKY), mapping with fluorescently labeled genomic clones (FISH), comparative genomic hybridization ... Analysis of genes within the deleted region of chromosome 4 demonstrated decreased expression of the cell cycle inhibitory ... We used Spectral Karyotyping (SKY), mapping with fluorescently labeled genomic clones (FISH), comparative genomic hybridization ... 11.0 of the cell cultures. Expression array analysis demonstrated increased expression of a proposed oncogene, Kruppel-like ...
Spectral karyotype[edit]. Spectral karyotyping is an image of colored chromosomes. Spectral karyotyping involves FISH using ... which require dividing cells and requires labor and time-intensive manual preparation and analysis of the slides by a ... and miRNA in tissues and cells. FISH is used by examining the cellular reproduction cycle, specifically interphase of the ... FISH on sperm cells is indicated for men with an abnormal somatic or meiotic karyotype as well as those with oligozoospermia, ...
  • Genetic instability can result from changes in chromosome structure, through errors in DNA metabolism, repair, recombination, or other rearrangements of the DNA sequence ( 2 ), or from misregulation of the cell cycle, for example, uncoupling DNA replication from cell division ( 3 ) or centrosomal duplication from division ( 4 ). (
  • If cells bypass this stage, they continue to grow until telomeres become critically short and cells enter crisis phase, characterized by generalized chromosome instability that provokes mass apoptosis ( 5 ). (
  • Chromosome mis-segregation leads to aneuploidy, a condition in which cells harbor an imbalanced chromosome number. (
  • Stable expression of core protein induced frequent chromosome translocations in cultured cells and in transgenic mice. (
  • Chromosome fragmentation is a major form of mitotic cell death which is identifiable during common cytogenetic analysis by its unique phenotype of progressively degraded chromosomes. (
  • In this analysis the phenomena of chromosome fragmentation and PCC are reviewed and their similarities and differences are discussed in order to facilitate differentiation of the similar morphologies. (
  • According to the recently established genome theory, stochastic chromosome alteration plays a key role in both organismal and in somatic cell evolution. (
  • Chromosome fragmentation is a non-apoptotic form of mitotic cell death, and is observed from an array of cell lines and patient tissues. (
  • New data also illustrates that it is a programmed cell death as PARP is readily degraded during the process of chromosome fragmentation, where chromosomal fragmentation occurs as a general response to cellular stress including oxygen stress, ER stress and various pathological stresses (Stevens et al submitted). (
  • The identification of chromosome fragmentation as a form of cell death is new to the field, but the morphological description of chromosome fragmentation is not. (
  • In regards to reassessing the classification of chromosomal abnormalities, the further analysis of chromosome fragmentation, a form of mitotic cell death, has renewed interest in phenomena such as chromosome shattering and pulverization. (
  • We show using a 4-hydroxytamoxifen-inducible Cre that the loss of PRMT1 in MEFs leads to a higher incidence of chromosome losses, gains, structural rearrangements, and polyploidy, as documented by spectral karyotyping. (
  • Human malignant melanoma cell line UACC903 is resistant to apoptosis while chromosome 6-mediated suppressed cell line UACC903(+6) is sensitive. (
  • Using these two cell lines, we previously identified the chromosome 6-encoded tumor suppressor gene connexin 43 which partially explains the suppression of tumorigenic phenotypes as a consequence of the introduced chromosome 6 [ 16 , 17 ]. (
  • Recently, two Nobel prizes have been awarded for discoveries where HeLa cells played a central role, namely the link between human papilloma virus and cervical cancer (2008, Harald zur Hausen) and the role of telomerase in preventing chromosome degradation (2011, Elizabeth Blackburn, Carol Greider, and Jack Szostak). (
  • The medial portion of chromosome 4 was deleted in 66.0% +/- 12.0 of the cell lines. (
  • Duplication of chromosome 4 at 10 to 35 cM occurred in 68.0% +/- 11.0 of the cell cultures. (
  • Analysis of genes within the deleted region of chromosome 4 demonstrated decreased expression of the cell cycle inhibitory factor p16 at 39 cM, the NR4A3 receptor involved in apoptosis at 42.7 cM, the differentiation factor Forkhead Box D3 at 45 cM and the apoptotic factor Cathepsin D at 50 cM. (
  • [9] FISH allows the analysis of a large series of archival cases much easier to identify the pinpointed chromosome by creating a probe with an artificial chromosomal foundation that will attract similar chromosomes. (
  • Services include conventional chromosome analysis such as G-banding (Human tissue only) and fragile site analysis as well as more specialized techniques such as Spectral karyotyping (SKY) and fluorescent in situ hybridization (FISH). (
  • G-Banding analysis (Human samples only) to identify chromosome number as well as chromosome aberrations including translocations, insertions, deletions or duplications in cells. (
  • FISH analysis is used for gender selection, aneuploidy screening (chromosome number), single gene disorders, and translocations. (
  • The effect of functional inactivation of TP53 by HPV-E6 transformation on the induction of chromosome aberrations by gamma rays in human tumor cells. (
  • These studies demonstrate that E6 transformation of human tumor cells will influence both the frequency and types of chromosome aberrations observed after radiation exposure, and that these effects are related to the expression of potentially lethal damage. (
  • However, the ability to quantify and visualize differences in chromosome number between experimentally-defined groups (e.g. control vs treated) obtained from single-cell experiments is currently limited by the lack of user-friendly software. (
  • The web interface allows users to upload molecular cytogenetic or processed single cell whole-genome sequencing data in a cell-by-chromosome matrix format and automatically generates visualizations and summary statistics that reflect the degree of numeric chromosomal variability. (
  • As a step in assessing the utility of this approach, we derived primary embryonic fibroblast populations and embryonic stem cell lines from mice that carried two different fluorescent protein genes as alleles at the chromosome 6 locus, ROSA26. (
  • As a first step in assessing the utility of the allelic marker approach in mammals, we derived cell lines from mice that carried two different fluorescent protein (cyan and yellow) genes as alleles at the widely expressed ROSA26 locus, which is on chromosome 6 [ 12 ]. (
  • Chromosome banding and spectral karyotyping (SKY)[ 11 ] are low-resolution techniques used to detect large-scale chromosomal features. (
  • SUV39DN ES cells and MEFs showed a significant proportion of very long telomeres at both p and q chromosome arms, which were not present in the wild-type controls ( Fig. 1c,d ). (
  • The majority of human tumour cells are aneuploid owing to an underlying chromosome instability phenotype. (
  • While the genetic lesions that cause chromosome instability remain undefined, mouse ES cells harbouring homozygous adenomatous polyposis coli ( APC ) mutations are frequently tetraploid. (
  • Therefore, to determine whether APC mutations can initiate chromosome instability in human cells, we expressed N-terminal APC fragments in HCT-116 cells, a near diploid colon cancer cell line with two wild-type APC alleles. (
  • Adeyinka A, Kytola S, Mertens F, Pandis N, Larsson C (2000) Spectral karyotyping and chromosome banding studies of primary breast carcinomas and their lymph node metastases. (
  • We have used cytogenetic, flow cytometry and cell culture tools to investigate chromosomal rearrangements and clonality during cancer development using the murine sarcoma TG180 model, and also molecular biology techniques to establish a correlation between chromosome instability and telomerase activity, since telomeres are highly affected during cancer evolution. (
  • Specific chromosome aberrations (marker chromosomes) and activated regions (rDNAs) were ubiquitous in the karyotype, suggesting that the conservation of these patterns may be advantageous for tumor progression. (
  • Our data reinforce the notion that the sarcoma cell evolution converges from a highly unstable karyotype to relatively stable and functional chromosome rearrangements, which are further enabled by telomerase overexpression. (
  • The presence or absence of specific chromosomes from the chromosome set, the increasing number of chromosome copies or the presence of some marker chromosomes can determine whether a cell line is more or less invasive, thereby directing the type of treatment to be adopted. (
  • Human cells with a chromosome number different from 46 or with an abnormal complement of chromosomes that add up to 46 are aneuploid. (
  • This imbalance of chromosome number may be in germ cells or somatic cells. (
  • Cells with less than a diploid chromosome content are referred to as having hypodiploid aneuploidy. (
  • Chromosome analysis was done by metaphase chromosome banding using conventional Giemsa banding technique. (
  • Spectral karyotyping revealed a largely normal diploid karyotype (in greater than 95% of cells) with a visibly shorter chromosome 20 contig. (
  • High density SNP array analysis also revealed few genomic anomalies in BIN-67 cells, which included loss of heterozygosity of an estimated 16.7 Mb interval on chromosome 20. (
  • Colon cancer cells frequently display minisatellite instability (MIN) or chromosome instability (CIN). (
  • Human tumour cells often have abnormal chromosome complements suggesting that aneuploidy may also cause cancer. (
  • European investigations demonstrated that detection of increased frequencies of chromosome aberrations as well as cells with micronucleus in human blood correlated with increased risk of tumor development [2] [3]. (
  • Several lines of evidence strongly indicate that aneuploidy triggers genome instability, ultimately generating cells with complex karyotypes that arrest their proliferation. (
  • What we do know is that, at the level of the cancer cell, the changes that end up causing unregulated proliferation (tumorigenesis) and somatic dispersal (metastasis) are the consequence of the altered functions of three types of gene products 19 that together control six cell physiologies 20 . (
  • The second type of cancer gene encodes tumor suppressors , which, as their name suggests, act to limit cell proliferation, acting in essence like anti-oncogenes. (
  • SIRT1 is a member of the sirtuin family of type III histone deacetylases and plays a role in many biological processes, including cell death and survival, cell proliferation and differentiation, neurological functioning, metabolism, calorie restriction, aging, and cancer [ 14 - 23 ]. (
  • Taken together, these data show that PRMT1 is required for genome integrity and cell proliferation. (
  • Cell cycle analysis indicated that tumor spheres were of a high proliferation state.We could not find any noticeable difference in the number of chromatosomes between the SFM group and the SSM group. (
  • Expression of E6 increased cell proliferation and induced epidermal hyperplasia. (
  • Overexpression of CCND1 results in increased proliferation and disruption of the normal cell cycle [ 13 ]. (
  • Alterations in genes and regulatory elements critical to cell cycle control lead to uncontrolled cell growth and proliferation, the common signature of all cancers. (
  • All together, the results of these and other studies suggest that there is no universal mechanisms by which plant-derived, synthetic, and endogenous cannabinoids affect cell viability and proliferation of glioma cells. (
  • Dietary manipulation of folate has been referred to as a 'double edge sword' because pharmacological depletion of folate by antifolate drugs prevents cancer cell proliferation but might induce genetic and epigenetic damage and consequent transformation in non-cancerous cells. (
  • Gene expression analysis revealed abnormally regulated stress response, cell proliferation, and signal transduction pathways in ARTEMIS-defective MSCs. (
  • Since inflammatory mediators, including members of the interleukin-6 (IL-6) cytokine family, suppress proliferation of normal epithelial cells, we hypothesized that epithelial cells must develop mechanisms to evade this inhibition during the tumorigenesis. (
  • OSM and macrophage-derived cytokines suppressed proliferation of normal epithelial cells, but reduced inhibition or even stimulated proliferation was noted for preneoplastic cells. (
  • Cell Proliferation. (
  • We hypothesized that viable tumor phenotypes possess common and highly stable karyotypes and their proliferation is facilitated by an attuned high telomerase activity. (
  • Its over-expression inhibited tumour growth and invasion and its down-regulation promoted cell proliferation and invasion. (
  • During division, these cells frequently exhibit lagging chromosomes at both metaphase and anaphase, suggesting defects in the mitotic apparatus or kinetochore. (
  • Dicentric anaphase chromatin bridges and structurally altered chromosomes with consistent long arms and variable short arms, as well as the presence of gene amplification, suggested the occurrence of breakage-fusion-bridge cycles. (
  • The karyotypes of oral squamous cell carcinomas (OSCC) are complex, often near triploid, and are composed of multiple numerical and structural abnormalities, including deletions, balanced and unbalanced translocations, isochromosomes, dicentric chromosomes, and homogeneously staining regions ( 5 ). (
  • In a human cell, the genetic information is encoded in 46 chromosomes. (
  • Spectral karyotyping (SKY) to identify chromosomes or chromosomal segments that are structurally and numerically altered in cells. (
  • The first step is to quantify the number of chromosomes per cell. (
  • Chromosomal aberrations include the loss or gains of partial or whole chromosomal arms on several chromosomes and are a hallmark of cancer cells. (
  • This technique allows for karyotyping at higher speeds than with previous methods and was shown to be accurate using Chinese hamster chromosomes. (
  • A microfluorometer can be used to estimate amounts and distributions of chemical components in individual cells or in chromosomes. (
  • anaphase The stage of mitosis and meiosis that occurs after metaphase and before telophase, when the replicated chromosomes are segregated and each of the sister chromatids are moved to opposite sides of the cell. (
  • Normal human somatic cells have 23 pairs of chromosomes. (
  • In the case of aneuploidy, the cell may gain or lose one or more chromosomes. (
  • Cells with more than a diploid but less than a tetraploid complement of chromosomes are referred to as having hyperdiploid aneuploidy. (
  • The DNA index of a tumour indicates the degree of aneuploidy and it is calculated as modal number of chromosomes of the tumour population divided by the reference number of chromosomes of the normal diploid cells. (
  • The stage of cell division in which the spindle forms and chromosomes line up along the center of the cell. (
  • A total of 30 metaphase chromosomes were analyzed and the results showed that the patient had 46 XX karyotype (Figure 1) known as de la Chapelle syndrome with no evidence of other structural/numerical chromosomal abnormalities including mosaicism. (
  • 23 pairs of chromosomes are present into human somatic cells called a haploid set of chromosomes (n=23). (
  • Therefore, a total of 46 chromosomes are present in a human somatic cell. (
  • 22 pairs of autosomal and a pair of sex chromosomes are present in every cell. (
  • The behaviour of the chromosomes during the mitosis or somatic cell division (growth and development). (
  • The behaviour of the chromosomes meiosis or germ cell division (reproduction). (
  • We used immunohistochemistry combined with classical cytogenetic analysis and spectral karyotyping to investigate the chromosomal segregation defects in cultured oral squamous cell carcinoma cells. (
  • Cytogenetic analysis of head and neck squamous cell carcinoma (HNSCC) established several biomarkers that have been correlated to clinical parameters during the past years. (
  • Although they have been subjected to cytogenetic analysis, their chromosomal abnormalities have not been carefully characterized, and their differential cytogenetic profiles have not yet been established. (
  • Cytogenetic analysis showed a near-tetraploid karyotype originated by endoreduplication. (
  • In the cytogenetic analysis "cell" is one of the major constituents. (
  • Fluorescence in-situ hybridization (FISH) analysis using up to three-fluorescent colors to detect and localize genes, gene sets, fusion genes, telomere-specific sequences, centromere-specific sequences and specific chromosomal regions. (
  • The cell is then fixed on a slide for analysis later in the case of FISH (fluorescent in situ hybridization) or placed in a small container in the case of CGH (comparative genomic hybridization). (
  • Furthermore, no freely-available software exists for the processing of chromosomal count data derived from locus specific fluorescent in situ hybridization (FISH) or spectral karyotyping (SKY). (
  • Microfluorimetry is an adaption of fluorimetry for studying the biochemical and biophysical properties of cells by using microscopy to image cell components tagged with fluorescent molecules. (
  • Flow microfluorimetry (FMF) can also be used to determine different populations of cells using fluorescent markers with small cell samples. (
  • This same concept can also be applied to distinguish between cell types using a suitable fluorescent dye which varies depending on purpose and is a critical technique in modern cell biology and genomics. (
  • Aneuploidy can be detected with the help of traditional metaphase cytogenetics, interphase cytogenetics (fluorescent in situ hybridisation (FISH), multicolour FISH, spectral karyotyping, and comparative genomic hybridisation techniques (CGH)), flow cytometry (FCM), and image cytometry (ICM). (
  • Tissue specimens are examined by CD45 immunohistochemistry and fluorescent in situ hybridization to detect hematopoietic and nonhematopoietic cells. (
  • Fluorescent protein fusions to ClpV of T6SSs targeting bacterial cells showed that this protein localizes to dynamic foci, which is dependent on components of the T6SS [9, 15, 16]. (
  • The cell line was cytogenetically characterized using array CGH, spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH). (
  • A range of 19 to 26 metaphases of the MCF7, T47D, BT474 and SKBR3 cell lines was studied using conventional (G-banding) and molecular cytogenetic techniques (multi-color fluorescence in situ hybridization, M-FISH). (
  • Fluorescence activated cell sorting (FACS) showed that the vast majority of cells in each line expressed the two marker proteins at similar levels, and that populations exhibited expression noise similar to that seen in bacteria and yeast. (
  • Cytogenetic studies using comparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH) have already associated chromosomal aberrations with non-small cell lung cancer (NSCLC) [ 8 , 9 ]. (
  • Established in 2005, WiCell's cytogenetics laboratory provides genetic testing including karyotyping by G-band, fluorescence in situ hybridization (FISH), a rapid FISH test (fast FISH), array comparative genomic hybridization (aCGH), spectral karyotyping (SKY) and short tandem repeat (STR) analysis. (
  • Array CGH and fluorescence in situ hybridization analysis was used to validate CGH results on selected cases. (
  • Dichromatic mouse embryonic cells provide a novel system for studying genetic/karyotypic stability and factors influencing expression from allelic genes. (
  • This technique enables clinicians to monitor and diagnose diseases using minute samples such as embryonic cells, blood & tissue. (
  • One of the most striking manifestations of genetic instability in cancer cells is the variation observed in the karyotypes of different cells, even within the same tumor. (
  • As a starting point for the investigation of genetic markers predicting radiosensitivity in tumor cells, irradiation experiments were carried out and radiation responses of CAL 33 have been determined. (
  • The clear similarities between stem cell and cancer stem cell genetic programs are nonetheless the basis of a recent proposal that some cancer stem cells could derive from human adult stem cells. (
  • We used designer nucleases to edit the underlying mutation in prkdc, the gene encoding DNA-PKcs, and demonstrated that genetic correction of the disease locus rescued DNA-PK dependent signaling, restored normal radiosensitivity, and enabled T-cell maturation and polyclonal T-cell receptor recombination. (
  • In vitro disease modeling with induced pluripotent stem cells (iPSCs) provides a practical alternative, and the study of several disorders has benefitted enormously from the convergence of three key technologies: modern genomics that links genetic variants to disease phenotypes, the ability to generate patient-specific iPSCs that can be differentiated into cell types affected by disease, and powerful tools for editing complex genomes [ 1 , 2 ]. (
  • In this issue of the JCI , two independent groups of researchers have simultaneously identified the genetic cause of a human syndrome marked by growth retardation, adrenal insufficiency, and natural killer cell deficiency (pages 814 and 821 ). (
  • In this issue of the JCI, two independent groups of researchers have simultaneously identified the genetic cause of a human NK cell deficiency as mutation in the MCM4 gene, encoding minichromosome maintenance complex component 4. (
  • Effective design and interpretation of molecular genetic studies performed using HeLa cells require accurate genomic information. (
  • Preimplantation genetic diagnosis is an advanced reproductive technology in which a single cell, or blastomere, is removed from the embryo and evaluated by genetic testing for various reasons. (
  • A small micromanipulation pipette is then used to gently pull out a single cell, which can be used for genetic testing. (
  • Pre implantation Genetic Diagnosis (PGD) is the genetic testing of a single cell from an embryo prior to transfer of the embryo to the uterus. (
  • The cell is then sent for genetic testing. (
  • During malignant progression, cells accumulate multiple genetic and epigenetic alterations that cause loss of at least one anti-oncogenic function. (
  • Although our studies were primarily motivated by an interest in LOH in mouse tissues, studies on genetic stability and allelic gene expression in mouse ES cells are of interest in their own right. (
  • We, therefore, hypothesized that prostate cells might be highly susceptible to genetic, epigenetic and phenotypic changes consequent to folate restriction. (
  • This study demonstrates that prostate cells are highly susceptible to genetic and epigenetic changes consequent to mild folate depletion as compared to cells grown with supraphysiological amounts of folate (2 μM) routinely used in tissue culture. (
  • Unrepaired DNA double-stranded breaks (DSBs) cause chromosomal rearrangements, loss of genetic information, neoplastic transformation or cell death. (
  • Evidence such as tumour specific aneuploidy, presence of aneuploidy in various preneoplastic conditions, increased frequency of genetic instability in aneuploid cell lines compared with diploid cells, and mutation of mitotic checkpoint genes suggests that aneuploidy possibly plays an active role in carcinogenesis. (
  • Tumourigenicity, in vitro growth characteristics, genetic and genomic anomalies, and sensitivity to standard and novel chemotherapeutic treatments were investigated in the unique SCCOHT cell line, BIN-67, to provide further insight in the biology of this rare type of ovarian cancer. (
  • Prior to cell division, the replicated genome is segregated such that the daughter cells receive all the genetic information required for further growth and development. (
  • The right number of blastomeres removed is a controversial topic as two cells allow for more genetic material and more accurate results. (
  • Molecular and Genetic analysis require a significant amount of DNA, which we would not have without PCR. (
  • Genome wide analyses using cDNA-expression arrays, CGH-arrays and epigenetic approaches, allowed us to identify multiple coding and non-coding (miRNAs) loci underlying genetic susceptibility in these lymphomas. (
  • Villa-Morales M, Santos J, Pérez-Gómez E, Quintanilla M and Fernández-Piqueras J (2007) A role for the Fas/FasL system in modulating genetic susceptibility to T-cell lymphoblastic lymphomas. (
  • In addition, we elucidate for the first time the contribution of these aspects to consequent phenotype changes in epithelial cells. (
  • Little is known about the fate of normal human mammary epithelial cells (HMECs) that lose p53 function in the context of extracellular matrix (ECM)-derived growth and polarity signals. (
  • Lung cancer, which is also known as carcinoma of the lung or pulmonary carcinoma, and is derived from epithelial cells, is a malignant lung tumour characterised by uncontrolled cell growth in the tissues of the lung. (
  • This study compared the cytokine responses of normal epithelial cells to that of premalignant cells. (
  • Short-term primary cultures of epithelial cells were established from bronchial brushings. (
  • Normal epithelial cells respond strongly to OSM, IFNγ and EGF, and respond moderately to IL-6, and do not exhibit a detectable response to LIF. (
  • Lung epithelial cells are consistently exposed to many irritants and pathogens. (
  • Histologically, the tumours have a sheet-like arrangement of small, closely packed epithelial cells with 80% of cases containing variably sized follicle-like structures. (
  • In 2008 she completed her PhD at the EPFL in Lausanne with a work demonstrating the plasticity and stemness of thymic epithelial cells. (
  • When oncogenes are activated , either through mutation or duplication (amplification), or - most often - through chromosomal rearrangements 13,14 , they promote cell division (mitosis). (
  • While aberration frequencies were similar at early harvest times, there was evidence for a subpopulation of more heavily damaged cells in the E6-transformed cells that cycled into late mitosis. (
  • Cells with a monochromatic phenotype were present at frequencies on the order of 10 -4 and appeared to be produced at a rate of approximately 10 -5 variant cells per mitosis. (
  • Subsequent mis-segregation during mitosis of a trisomic cell can produce a disomic cell where both homologues were derived from the same parental homologue (UPD) [ 7 ]. (
  • organize the stages of cell division sequentially for mitosis and meiosis. (
  • We show that cells expressing N-APC mutants exit mitosis prematurely in the presence of spindle toxins, consistent with a spindle checkpoint defect. (
  • Furthermore, the high levels of pH2A.X staining seen in curcumin-treated mitotic but not interphase cells suggest that aberrant mitosis may result in DNA damage. (
  • The cytogenetics is a branch of genetics that includes the study of chromosomal structure, function, properties, behaviour during the cell division (mitosis and meiosis) and its involvement in a disease condition. (
  • Oncogenic signals do not necessarily result in cell transformation because they are tightly linked to various tumor suppressive mechanisms that lead to apoptosis and premature senescence ( 1 ). (
  • 100-fold increased activity in hereditary c.1380delA CDH1 gastric cancer cells inducing apoptosis most effectively in cells with deficient CDH1 function. (
  • Oncogenes comprise the first type of cancer gene, and consist of growth factors, growth factor receptors, signaling molecules, transcription factors, cell death (apoptosis) regulators, or molecules involved in chromatin remodeling 21 . (
  • Our bioinformatic analysis mapped these genes to differential molecular pathways that predict resistance and sensitivity of UACC903 and UACC903(+6) to apoptosis respectively. (
  • These results demonstrated the differential molecular pathways underlying survival and apoptosis of UACC903 and UACC903(+6) cell lines. (
  • High resistance to treatment is a unique hallmark of malignant melanoma, although the mechanisms by which melanoma cells protect themselves against induced apoptosis remains largely unknown [ 3 ]. (
  • Restoration of Fragile Histidine Triad (FHIT) Expression Induces Apoptosis and Suppresses Tumorigenicity in Lung and Cervical Cancer Cell Lines Proceedings of the National Academy of Sciences of the United States of America. (
  • Restoration of Fhit expression induced apoptosis in all Fhit-negative cell lines, with Calu-1, H460, and A549 being the most susceptible among the lung cancer cell lines and SiHa cells among cervical carcinomas. (
  • Activation of caspase-8 was always associated with Fhit-mediated apoptosis, and in vivo tumorigenicity was either abolished by FHIT gene transfer (in H460 and SK-Mes cells) or strongly suppressed (in A549 and SiHa cells). (
  • Members of the Foxo family of transcription factors are also regulated by Akt, thus linking control over homing and migration of T cells, as well cell cycle entry, apoptosis, and DNA damage and oxidative stress responses, to PI3K signaling. (
  • Apoptosis and cell death by R(+)-MA were not affected by antagonists of cannabinoid receptors (CB 1 , CB 2 ) and vanilloid receptor 1. (
  • In contrast, early passage p53 − HMEC cells proliferated in rECM until day 6 but then underwent apoptosis on day 7. (
  • Treatment of early passage p53 − HMEC-E6 cells with either α3- or β1-integrin function-blocking antibodies inhibited rECM-mediated growth arrest and induction of apoptosis. (
  • Our results indicate that suppression of p53 expression in HMECs by HPV-16 E6 and ODNs may sensitize cells to rECM-induced apoptosis and suggest a role for the α3/β1-heterodimer in mediating apoptosis in HMECs grown in contact with rECM. (
  • 2009). Activation of ATM/Chk1 by curcumin causes cell cycle arrest and apoptosis in human pancreatic cancer cells. (
  • Gene expression studies of ACC have identified a gene signature that is associated with early development, cell cycle regulation, apoptosis, myoepithelial differentiation, and extracellular matrix ( 20 , 21 ). (
  • The invasive potential of tumor cells in vitro was observed by Matrigel invasion assay system. (
  • Many tumor cells bypass the apoptotic machinery through mechanisms that may involve dysregulation of pro- and anti-apoptotic genes [ 4 ]. (
  • T lymphocytes play an important role in adaptive immunity against invading pathogens or in fighting tumor cells. (
  • [2] FISH can also be used to detect and localize specific RNA targets ( mRNA , lncRNA and miRNA ) in cells, circulating tumor cells, and tissue samples. (
  • Cells, circulating tumor cells (CTCs), or formalin-fixed paraffin-embedded (FFPE) or frozen tissue sections are fixed, then permeabilized to allow target accessibility. (
  • Single-cell genomics analysis is also necessary when the number of cells available is limited to few or one, such as prenatal testing samples ( 7 , 8 ), circulating tumor cells ( 9 ), and forensic specimens ( 10 ). (
  • A totally different use of the ability to detect tumor cells with excessive sensitivity is the analysis of tissues for the presence of a selected mutation that identifies the first tumor being handled. (
  • Review: polymerase chain response detection of micrometastases and circulating tumor cells: software to melanoma, prostate, and thyroid carcinomas. (
  • Then we analysed expressions of stem cell surface markers CD133 and CD44 among undifferentiated cell, post-differentiated cells and routine Colo205 cells under serum-supplemented culture condition by flow cytometry. (
  • Another use of microfluorometry is flow cytometry which uses the emission of fluorochrome molecules and usually a laser as a light source to create data from particles and cells. (
  • For example, E. coli bacteriophages lambda and T4 were able to be separated by flow cytometry which allowed for genomic analysis which was previously difficult. (
  • To determine whether induction of endogenous CerS1 is important in mediating mitochondrial tension signaling, we treated UM-SCC-22A cells using the known tension inducer, SoSe (5 M, 3 hours), and assessed its results on CerS1 mRNA/protein LY2109761 enzyme inhibitor great quantity, mitophagy, and cell loss of life. (
  • This arrest was associated with a decrease in the mRNA levels of core regulatory genes, PBK , BUB1 , and CDC20 as determined by microarray-analysis and verified by Real-Time PCR. (
  • In silico analysis of GluN2A mRNA revealed that there are 6 upstream open reading frames (uORFs) present in glun2A 59UTR [57]. (
  • This locus is frequently lost in a number of malignancies, and consistent loss of NOL7 through loss of heterozygosity and decreased mRNA and protein expression has been observed in tumors and cell lines. (
  • We have used a cell line, CAL 33, for the establishment of a cell culture model in order to perform functional analyses of interesting candidate genes and proteins. (
  • The PRMT1 and PRMT3 genes have been targeted in mouse embryonic stem (ES) cells using gene-trapping strategies ( 44 , 53 ). (
  • Combined analysis by SKY and FISH to determine whether rearrangement, deletion or amplification occurs in genes encoding tumor suppressors, cell cycle factors, factors involved in immunological responses, DNA-damage responses, drug resistance, transgene expression, etc. (
  • However, the RNA transcriptional profile determined by whole genome oligonucleotide microarrays [ 1 , 4 , 11 ] characterized all four-cell lines as luminal because of the expression of both ERα-regulated genes (e.g. (
  • The global gene expression program of the iMyc Eμ -1 cells and the expression of 768 "pathway" genes were determined with the help of the Mouse Lymphochip © and Superarray © cDNA micro- and macroarrays, respectively. (
  • In the present study, we treated primary glioblastoma, rhabdomyosarcoma, hepatocellular carcinoma and human embryonic carcinoma cells (NCCIT) with μ-molar concentrations of genistein and assessed mitotic index, cell morphology, global gene expression, and specific cell-cycle regulating genes. (
  • We then used gene expression arrays (Illumina) for genome-wide expression analysis and validated the results for genes of interest by means of Real-Time PCR. (
  • The results of the present study, together with the results of earlier studies show that genistein targets genes involved in the progression of the M-phase of the cell cycle. (
  • In this study, expression levels of the EMSY and CCND1 genes were investigated in 85 patients with non small cell lung cancer by Real Time PCR. (
  • Many studies conducted over several decades on benign and malignant melanocytic lesions as well as melanoma cell lines have implicated numerous genes in melanoma development and progression. (
  • The observation that CIN cells fail to undergo mitotic arrest following spindle damage suggested that mutations in spindle checkpoint genes may account for CIN. (
  • Isolation of adipose tissue-derived mesenchymal stem cell. (
  • Samples were clarified by sedimentation (600 × g , 5 minutes, room temperature), the resulting cell suspension filtered through a 40 mm 2 nylon filter (Becton Dickinson, San Jose, CA), plated onto tissue culture plastic (10 3 cells/cm 2 ), allowed to adhere (24 hours), and washed twice with PBS (10 mL). (
  • Stat 3 regulates the growth of these tissue cells by responding to signals from outside the cell. (
  • Cyclin D1 increases the rate of growth and division of the cell and the formation of new blood vessels around the tissue. (
  • However, in selected preclinical and clinical conditions, coexistence of cells in different activation states and unique or mixed phenotypes have been observed, a reflection of dynamic changes and complex tissue-derived signals. (
  • If tissue architecture permits, the cause of LOH would be suggested by the number and arrangement of mutant cells because LOH caused by mitotic crossing over produces a pair of neighboring monochromatic cells expressing different colors [ 11 ]. (
  • However, obtaining metaphase spreads for performing a karyotype is often difficult, especially when working with solid tumor biopsies and paraffin embedded, formalin fixed tissue. (
  • Senescence of vascular even muscle tissue cells (VSMCs) plays a part in aging aswell as age-related illnesses of the heart. (
  • ud Using physiologically relevant concentrations of curcumin (5-10μM), achievable in the gut tissue following oral ingestion, cell cycle analysis showed that treatment for 12 hours results in significant G2/M arrest in all five cell lines. (
  • However, molecular medicine is profoundly altering the approach to tissue analysis. (
  • Using three newly established model cell lines from patient biopsy specimens, designated T-cell breast lymphoma (TLBR)-1 to -3, we characterized the phenotype and function of these tumors to identify mechanisms of cell survival and potential therapeutic targets. (
  • Specifically, the parental UACC903 cell line demonstrates rapid population-doubling time, focus formation in monolayer culture, anchorage-independent growth, and rapid formation of s.c. tumors in athymic nude mice. (
  • Restoration of Fhit expression in cell lines derived from these tumors has however yielded conflicting results, prompting the need for careful evaluation of the oncosuppressive potential of FHIT. (
  • We have recently shown that gene-targeted iMyc Eμ mice that carry a His 6 -tagged mouse Myc cDNA, Myc His , just 5' of the immunoglobulin heavy-chain enhancer, Eμ, are prone to B cell and plasma cell tumors. (
  • The Ewing sarcoma family of tumors (ESFT) is a group of malignant small round cell neoplasms of bones and soft tissues closely histogenetically related. (
  • Subsequent analysis, using comparative genomic hybridization (CGH) of these tumors have reported recurrent chromosomal gains of 16p, 17q, 19, and 22q13 and losses at 6q23-qter, 12q12-q13, 13q21-q22, and 19 ( 12 , 13 ). (
  • The term "genomic instability" describes complex of various molecular mechanisms and their effects leading stable genome of normal cell to unstable, characteristic for tumors [1]. (
  • Molecular analysis of a number of tumors of the transitional epithelium in the urinary bladder has demonstrated that the a number of lesions come up from a single progenitor cell that seeds the bladder mucosa, explaining the excessive threat for recurrence encountered in these patients. (
  • Oral squamous cell carcinomas are characterized by complex, often near-triploid karyotypes with structural and numerical variations superimposed on the initial clonal chromosomal alterations. (
  • A well-characterized example is squamous cell carcinomas of the oral cavity. (
  • Although SCCOHT is morphologically similar to small cell carcinomas from other sites, its common expression of WT1 and lack of thyroid transcription factor (TTF)-1 allows it to be distinguished from other small cell cancers[ 8 ]. (
  • Stem cells are characterized by their self-renewal ability and differentiation potential ( 1 ) and can be divided into embryonic and adult stem cells. (
  • Stem cells and cancer stem cells share certain features such as self-renewal and differentiation potential. (
  • Cell differentiation. (
  • For adipogenic differentiation, cells were cultured in MEM plus 10% FCS, 0.5 mmol/L 3-isobutyl-1-methylxanthine, 0.5 mmol/L hydrocortisone, 1 mmol/L dexamethasone, 200 mmol/L indomethacin, and 50 μg/mL gentamicin for 2 weeks. (
  • To induce cell differentiation, tumour spheres were cultivated without EGF and bFGF in the presence of 10% serum. (
  • It is clear, however, that in many cases the neoplastic transformation of blood cells often involves either the failure or reversal of termination differentiation, resulting in primitive "cancer stem cells" (CSCs), which divide in an unregulated manner. (
  • Studying the molecular pathology of human disease is often difficult due to the limited availability of particular primary cells, their limited lifespan, or because complex developmental differentiation procedures cannot be easily followed in vivo . (
  • The iMyc Eμ -1 cell line, which demonstrated the typical cytological features of mouse LBL (Fig. 1A top), was derived from a primary IgM + LBL (Fig. 1A bottom) that exhibited moderate plasmacytic differentiation potential in situ (not shown). (
  • Finally, the development of mathematical approaches has permitted a more complete picture of the dynamic nucleus, which can further our understanding of critical developmental processes, such as cell specification and differentiation. (
  • Paola graduated in Medicine and Surgery from the University of Milan and subsequently moved to the Sanford-Burnham Institute in La Jolla, California where she worked on differentiation of human embryonic stem cells. (
  • Schematic diagram showing stages of T-cell differentiation. (
  • Lymphomas can be related to precursor B-cell, mature B-cell, and secretory B-cell (plasma cell) stages of differentiation. (
  • We used aCGH analysis to identify mechanisms driving taxane resistance. (
  • An aCGH profile demonstrated a loss of mitotic pathways in the resistant cell lines indicating a potential theranostic pathway. (
  • Finally, although gene expression profiling and aCGH studies have classified these four cell lines as luminal, our results suggest that they are heterogeneous at the cytogenetic level. (
  • Because MD Anderson is one of the largest cancer centers in the world, many special areas of instruction can be presented, such as Spectral Karyotyping, home-brew FISH probe making and comparative genomic hybridization through aCGH. (
  • The story of the cytogenetics was started unknownly in the mid 18's when Karl Wilhelm von Nageli, a Swiss botanist, who first observed the plant cell in the year 1842. (
  • Zitzelsberger, H. Establishment and Molecular Cytogenetic Characterization of a Cell Culture Model of Head and Neck Squamous Cell Carcinoma (HNSCC). (
  • Bauer VL, Hieber L, Schaeffner Q, Weber J, Braselmann H, Huber R, Walch A, Zitzelsberger H. Establishment and Molecular Cytogenetic Characterization of a Cell Culture Model of Head and Neck Squamous Cell Carcinoma (HNSCC). (
  • The molecular mechanisms regulating self-renewal of leukemia stem cells remain poorly understood. (
  • 1 Despite the importance of this process, the molecular mechanisms underlying the maintenance of stem cells is still poorly understood. (
  • HeLa is the most widely used model cell line for studying human cellular and molecular biology. (
  • We used Spectral Karyotyping (SKY), mapping with fluorescently labeled genomic clones (FISH), comparative genomic hybridization (CGH) on a BAC array, 5 kB NimbleGen CGH array, expression array, real time polymerase chain reaction and Western blot to analyze 15 primary adenocarcinoma and 9 pairs of high and low invasive cell cultures to detect molecular changes. (
  • The issue of genistein as a potential anti-cancer drug has been addressed in some papers, but comprehensive genomic analysis to elucidate the molecular mechanisms underlying the effect elicited by genistein on cancer cells have not been performed on primary cancer cells, but rather on transformed cell lines. (
  • Although induced pluripotent stem cells resemble embryonic stem cells both morphologically and functionally, the extent to which these cell lines are truly equivalent, from a molecular point of view, remains controversial. (
  • Direct Analysis in Real Time (DART™) high-resolution Orbitrap™ mass spectrometry (HRMS) in combination with Raman microscopy was used for the detailed molecular level characterization of explosives including not only the charge but also the complex matrix of binders, plasticizers, polymers, and other possible organic additives. (
  • To this end, we have assembled a set of molecular tests for hESC lines that assess identity, stability of the nuclear and mitochondrial genomes, histocompatibility profile, and the undifferentiated state of the cells. (
  • Therefore, malignancies of the immune system are not only interesting models of normal immune cells but are also excellent examples of the variety of molecular and immunological approaches for assessing cancers. (
  • Because the tumor suppressor p53 is known to play a critical role in this coupling ( 2 , 3 ), inactivating the mutations of p53 or the inability to activate p53 would increase susceptibility to oncogene-induced chromosomal aberrations and cell transformation. (
  • This "complexity of cancer progression can be understood as the result of multiple, sequential mutations, each of which has a relatively small but positive effect on net cell growth" 15 . (
  • In addition, colon cancer cells with APC mutations have weakened kinetochore-microtubule interactions. (
  • Consistent with Knudson's `two-hit' hypothesis, tumour cells from FAP patients and sporadic cases harbour mutations in both APC alleles, indicating that APC is a classic tumour suppressor. (
  • LOH or mutations at this locus cosegregated with melanoma susceptibility in familial melanoma kindred and 9p21 mutations have been observed in different cancer cell lines. (
  • The final stage of malignant cells is determined by multiple mutations that confer distinct fitness advantages, and occur stochastically. (
  • Somatic mutations in TP53 and the most common activating mutations in KRAS and BRAF were not found in BIN-67 cells by DNA sequencing. (
  • The latter 6 tumours commonly showed a mutant pattern (diffuse or null) in p53 immunohistochemistry, and 4 of the 6 tumours assessable for TP53 sequence analysis revealed mutations. (
  • Here we show that although they can be managed safely during the standard ex vivo expansion period (6-8 weeks), human mesenchymal stem cells can undergo spontaneous transformation following long-term in vitro culture (4-5 months). (
  • Human cells have two control points that regulate their life span in vitro , the senescence and crisis phases. (
  • In this study, we demonstrated that PBMCs obtained from HCV-infected patients showed frequent chromosomal aberrations and that HCV infection of B cells in vitro induced enhanced chromosomal breaks and sister chromatid exchanges. (
  • STAT3 inhibition, directly or by recovery of SHP-1, and cyclophosphamide-Adriamycin-vincristine-prednisone (CHOP) chemotherapy reagents, effectively kill cells of all three TLBR models in vitro and may be pursued as therapies for patients with breast implant-associated T-ALCLs. (
  • Due to the limited availability and lifespan of some primary cells, in vitro disease modeling with induced pluripotent stem cells (iPSCs) offers a valuable complementation to in vivo studies. (
  • We hence provide proof that the combination of two promising technology platforms, iPSCs and designer nucleases, with a protocol to generate T-cells in vitro, represents a powerful paradigm for SCID disease modeling and the evaluation of therapeutic gene editing strategies. (
  • Furthermore, our system provides a basis for further development of iPSC-derived cell products with the potential for various clinical applications, including infusions of in vitro derived autologous T-cells to stabilize patients after hematopoietic stem cell transplantation. (
  • Isogenic paclitaxel resistant (PACR) MDA‒MB‒231, paclitaxel resistant ZR75‒1 and docetaxel resistant (DOCR) ZR75‒1 cell lines were generated by incrementally increasing taxane dose in native cell lines in vitro . (
  • In vitro fertilization or intracytoplasmic sperm injection is still required in order to produce embryos that can be used for the cell biopsy. (
  • The purpose of this study was to establish and characterize a cell line, designated iMyc Eμ -1, for the in-depth evaluation of LBL in vitro . (
  • The iMyc Eμ -1 cells may provide a uniquely useful model system to study the growth and survival requirements of Myc -driven mouse LBL in vitro . (
  • We now report on a newly established LBL-derived cell line, iMyc Eμ -1, which was developed to study the growth and survival requirements of LBL in vitro . (
  • Mansur A, Israel A, Combelles CMH, Adir M, Racowsky C, Hauser R, Baccarelli AA, Machtinger R. Corrigendum: Bisphenol-A exposure and gene expression in human luteinized membrana granulosa cells in vitro. (
  • Our analysis of the HeLa gene expression profile revealed that several pathways, including cell cycle and DNA repair, exhibit significantly different expression patterns from those in normal human tissues. (
  • In this context, it can help define the spatial-temporal patterns of gene expression within cells and tissues. (
  • Mechanisms of tumor removed, tissues beyond intracavitary brachytherapy mammalian cells: Identities. (
  • In addition, LOH caused by mitotic crossing over might be discernable in tissues because this event produces a pair of neighboring monochromatic cells that are different colors. (
  • qRT-PCR was performed to detect expression of miR-454 in osteosarcoma cell lines and tissues. (
  • miR-454 was found to be down-regulated in osteosarcoma tissues and cell lines. (
  • Professor John Campbell is Associate Director of Tissues, Cells and Advanced Therapeutics at the Scottish National Blood Transfusion Service (SNBTS) in Edinburgh. (
  • To determine mitotic index, metaphase spreads were prepared from cells without prior colchicine treatment. (
  • A metaphase cell positive for the bcr/abl rearrangement (associated with chronic myelogenous leukemia ) using FISH. (
  • Twenty metaphase spreads were analyzed and karyotyped in accordance with ISCN 2013 (3). (
  • The IVM procedure is composed of manual removal of cumulus-oocyte complexes (COCs) from antral follicles and their culturing them under cell culture circumstances until attainment of metaphase II. (
  • Various "checkpoint controls" assure proper progression through the cell cycle, reducing the occurrence of spontaneous DNA damage ( 5 , 6 ). (
  • The progression of cells through the cell cycle is precisely regulated by multiple checkpoints at different transition phases of the cycle [ 1 - 5 ]. (
  • The loss of PRMT1 in MEFs leads to spontaneous DNA damage, cell cycle progression delay, checkpoint defects, aneuploidy, and polyploidy. (
  • While disruption of regulation by PTEN has the overt phenotype of cancer progression, PTEN also plays an important role in maintaining T cell tolerance at multiple stages within the T cell compartment. (
  • Myc -induced lymphoblastic B-cell lymphoma (LBL) in iMyc Eμ mice may provide a model system for the study of the mechanism by which human MYC facilitates the initiation and progression of B cell and plasma cell neoplasms in human beings. (
  • Thus, loss of INK4K function promotes RB inactivation through hyperphosphorylation, resulting in unconstrained cell cycle progression. (
  • ud Image analysis revealed impaired mitotic progression, and significantly higher levels of mitotic spindle abnormalities following curcumin treatment. (
  • one of the earliest hallmark events of cancer, and its progression is probably based on selection mechanisms under specific environments that lead to functional tumor cell speciation. (
  • We observed that EV-mediated transfer of cancer microRNAs was responsible for the transition from a mesenchymal to an epithelial phenotype (MET) in the treated fibroblasts as well as activation of cell cycle progression and cell survival pathways. (
  • Our findings indicate the importance of biosafety studies of mesenchymal stem cell biology to efficiently exploit their full clinical therapeutic potential. (
  • The TLBR cell lines closely resemble the primary breast implant-associated lymphomas from which they were derived and as such provide valuable preclinical models to study their unique biology. (
  • Endothelial cells from human umbilical veins were first cultured nearly four decades ago, initiating explosive growth in research in vascular biology and leading to major insights into angiogenesis, vasculogenesis, and tumor biology. (
  • This study underscores the importance of accounting for the strikingly aberrant characteristics of HeLa cells when designing and interpreting experiments, and has implications for the use of HeLa as a model of human biology. (
  • As the large-scale, comprehensive assays will be common in cancer research and prognosis, it is essential to perform integrative computational analysis of the heterogeneous data in order to obtain a systematic understanding of the underlying biology. (
  • Microfluorimetry has uses for many different fields including cell biology, microbiology, immunology, cell cycle analysis and "flow karyotyping" of cells. (
  • Cell lines derived prior to 9 August 2001 (currently about 20 available lines) can be examined using federal funds, and currently much of the available information on hESC biology has been generated using these funds and cell lines [ 1 ]. (
  • The biology of small cell ovarian carcinoma of the hypercalcemic type (SCCOHT), which is a rare and aggressive form of ovarian cancer, is poorly understood. (
  • In 2013 Paola was awarded a UCL-Rosetrees Excellence Fellowship to pursue her interest in epithelial stem cell biology and regenerative medicine at UCL. (
  • These findings indicate substantial changes in the state of telomeric heterochromatin in SUV39DN cells, which are associated with abnormal telomere elongation. (
  • Cancer stem cells have been identified and characterized in several tumor types, including acute myeloid leukemia, breast cancer, and glioblastoma ( 1 ). (
  • Independent Prognostic Significance of Monosomy 17 and Impact of Karyotype Complexity in Monosomal Karyotype/Complex Karyotype Acute Myeloid Leukemia: Results from Four ECOG-ACRIN Prospective Therapeutic Trials. (
  • Hepatitis C virus (HCV) infection is associated with the development of hepatocellular carcinoma and putatively also non-Hodgkin's B cell lymphoma. (
  • Anaplastic lymphoma kinase (ALK)-negative, T-cell, anaplastic, non-Hodgkin lymphoma (T-ALCL) in patients with textured saline and silicone breast implants is a recently recognized clinical entity for which the etiology and optimal treatment remain unknown. (
  • Numerous cases of rare T-cell ALK − anaplastic large cell lymphoma have recently been identified in women with textured silicone and saline breast implants. (
  • Breast implant-associated (BIA) T-cell anaplastic large cell lymphoma (ALCL) is a recently recognized clinical entity, with 80 cases identified worldwide to date and four disease-specific fatalities ( 1-15 ). (
  • Outcome for young high-risk aggressive B-cell lymphoma patients treated with CHOEP-14 and rituximab (R-CHOEP-14). (
  • For elderly patients with mantle cell lymphoma (MCL), there is no defined standard therapy. (
  • The B-Cell Lymphoma Moon Shot is revolutionizing the conventional medical research approach to rapidly translate findings into patient treatment options and develop personalized therapeutic strategies. (
  • We also provide follow up regarding a previously published case of histiocytic sarcoma with IGH/BCL2 fusion gene in which the patient subsequently developed follicular lymphoma and, later, diffuse large B-cell lymphoma. (
  • Interestingly, some of these gene alterations occur exclusively in the stroma that accompanies lymphoma cells ( ANXA1 and CD274 ). (
  • Detection of circulating cells harboring the bcl-2 translocation is a crucial prognostic predictor of relapse in patients with t(14:18)-constructive B-cell lymphoma handled with excessive-dose chemotherapy and autologous bone marrow or peripheral blood stem cell assist. (
  • MCF7 and T47D (ER+/HER2-) cells showed a less complex chromosomal make up, with more numerical than structural alterations, compared to BT474 and SKBR3 (HER2+) cells, which harbored the highest frequency of numerical and structural aberrations. (
  • Our study provides a comprehensive and specific characterization of complex chromosomal aberrations of MCF7, T47D, BT474 and SKBR3 cell lines. (
  • The E6-transformed cells also had higher levels of chromatid-type aberrations and sister chromatid exchanges, consistent with an additional defect in kinetics of repair of base damage that is associated with the E6 transformation. (
  • Holding cells noncycling for 24 h eliminated the elevated levels of chromatid-type aberrations and sister chromatid exchanges. (
  • Although aberrations at the two extremes of this spectrum are readily defined, comprehensive discernment of the complex and disperse mutational spectrum of cancer genomes remains a significant challenge for current genome analysis platforms. (
  • We found that short tandem repeat (STR) analysis, human leukocyte antigen (HLA) typing, single nucleotide polymorphism (SNP) genomic analysis, mitochondrial DNA sequencing, and gene expression analysis by microarray can be used to fully describe any hESC culture in terms of its identity, stability, and undifferentiated state. (
  • These results indicate that some of the chromosomal instability observed within these cancer cells might be the result of cytoskeletal defects and breakage-fusion-bridge cycles. (
  • We have begun to investigate the source of chromosomal instability in cultured cells derived from human OSCC. (
  • Aneuploidy leads to genome instability, which eventually produces cell cycle-arrested cells with complex karyotypes. (
  • Cells lacking NHEJ exhibit variable proliferative defects, hypersensitivity to ionizing radiation and other clastogens and spontaneous chromosomal instability. (
  • Curcumin treatment significantly increased the number of cells in M phase in 4 out of the 5 lines tested for this duration, and those with microsatellite instability (HCT116) were found to have a higher mitotic index than those with chromosomal instability. (
  • Application of SNP microarrays to the genome-wide analysis of chromosomal instability in premalignant airway lesions. (
  • While these assays certainly give indications of the undifferentiated state of the cells, they do not address other issues such as pluripotentiality or the degree of culture adaptation and genomic instability. (
  • Although both alleles are mutated in APC -defective colorectal cancer cells, these cells do not completely lack APC protein. (
  • Mitotic cell death is an important form of cell death, particularly in cancer. (
  • Functional network analysis revealed that the mitotic prometaphase was lost in the resistant cell lines. (
  • Thereafter, we compared the mitotic index of treated versus untreated cells and investigated the protein expression of key regulatory self renewal factors as OCT4, SOX2 and NANOG. (
  • Pre-treatment with caffeine abrogated mitotic arrest in these cell lines, indicating the involvement of the ATM/ATR kinases. (
  • However, here we show that CIN cells do undergo mitotic arrest in response to spindle damage. (
  • Although the maximum mitotic index achieved by CIN lines is diminished relative to MIN lines, CIN cells clearly have a robust spindle checkpoint. (
  • Significantly, we show here that expression of an APC mutant in MIN cells reduces the mitotic index following spindle damage to a level observed in CIN cells, suggesting that APC dysfunction may contribute to CIN. (
  • Senescence is associated with moderate telomere shortening and is characterized by cell cycle arrest and positive β-galactosidase staining at pH 6 ( 4 ). (
  • Stimulation of Rat1A cells with MYC accelerated their passage through G 1 /S. Moreover, MYC could force normal human fibroblasts to transit G 1 and S after treatment with N- (phosphonoacetyl)- l -aspartate (PALA) at concentrations that normally lead to arrest in S phase by checkpoint mechanisms. (
  • Instead, the cells subsequently appeared to arrest in G 2 . (
  • We found that cancer cells treated with genistein undergo cell-cycle arrest at different checkpoints. (
  • In contrast, human NCCIT cells showed over-expression of GADD45 A and G (growth arrest- and DNA-damage-inducible proteins 45A and G), as well as down-regulation of OCT4, and NANOG protein. (
  • Curcumin, a diet-derived chemopreventive and chemotherapeutic agent has been shown to induce G2/M cell cycle arrest, and previous studies suggested that microtubule depolymerisation may be linked to M-phase arrest. (
  • 2006). Activation of Checkpoint Kinase 2 by 3,3 '-Diindolylmethane Is Required for Causing G2/M Cell Cycle Arrest in Human Ovarian Cancer Cells. (
  • This is the first report of spontaneous transformation of human adult stem cells, supporting the hypothesis of cancer stem cell origin. (
  • In order to identify therapeutic leads selective for the HDGC subtype of gastric cancer, we compared gene expression profiles and drug phenotype derived from an oncology library of 1912 compounds between gastric cancer cells established from a patient with metastatic HDGC harboring a c.1380delA CDH1 germline variant and sporadic gastric cancer cells. (
  • Unsupervised hierarchical cluster analysis shows select gene expression alterations in c.1380delA CDH1 SB.mhdgc-1 cells compared to a panel of sporadic gastric cancer cell lines with enrichment of ERK1-ERK2 (extracellular signal regulated kinase) and IP3 (inositol trisphosphate)/DAG (diacylglycerol) signaling as the top networks in c.1380delA SB.mhdgc-1 cells. (
  • Differential high-throughput drug screening of c.1380delA CDH1 SB.mhdgc-1 versus sporadic gastric cancer cells identified several compound classes with enriched activity in c.1380 CDH1 SB.mhdgc-1 cells including mTOR (Mammalian Target Of Rapamycin), MEK (Mitogen-Activated Protein Kinase), c-Src kinase, FAK (Focal Adhesion Kinase), PKC (Protein Kinase C), or TOPO2 (Topoisomerase II) inhibitors. (
  • Integrated pharmacological and transcriptomic profiling of hereditary diffuse gastric cancer cells with a loss-of-function c.1380delA CDH1 mutation implies various pharmacological vulnerabilities selective to CDH1-deficient familial gastric cancer cells and suggests novel treatment leads for future preclinical and clinical treatment studies of familial gastric cancer. (
  • While we may feel cancer is a modern malady, glibly attributable to modern lifestyles or the environment (and for some cancers it certainly is), a paleopathological analysis of archeological remains suggests that cancer has been killing at roughly the same rate since ancient times 4 . (
  • Isolation and expansion tumor spheres from colorectal cancer cell line Colo205 cultured in serum-free medium(SFM) supplemented with human recombinant EGF and bFGF. (
  • Tumor spheres in which enriched cancer stem cells can be generated under serum-free culture condition with EGF and bFGF. (
  • In the present study, we have investigated the effect of Fhit reintroduction in seven lung cancer and three cervical cancer cell lines. (
  • The cell line originates from a cervical cancer tumor of a patient named Henrietta Lacks, who later died of her cancer in 1951 ( Skloot 2010 ). (
  • We detected digitized copy-number variations (CNVs) of a single cancer cell. (
  • We directly measured the genome-wide mutation rate of a cancer cell line and found that purine-pyrimidine exchanges occurred unusually frequently among the newly acquired SNVs. (
  • The overall aim of this study was to generate isogenic taxane-resistant breast cancer cell lines and elucidate the mechanisms that are driving resistance to taxanes in a pre-clinical model system. (
  • The breast cancer cell lines MDA-MB-231 and ZR75-1 (ATCC, Cedarlane Laboratories Ltd, Burlington, Canada) were cultured as monolayer in DMEM supplemented with 10% foetal calf serum, 10 mM glutamine and penicillin and streptomycin. (
  • The MCF7 (ER+/HER2-), T47D (ER+/HER2-), BT474 (ER+/HER2+) and SKBR3 (ER-/HER2+) breast cancer cell lines are widely used in breast cancer research as paradigms of the luminal and HER2 phenotypes. (
  • We applied the layered models to the integrated datasets of NCI-60 cancer cell lines and validated the results with large-scale statistical analysis. (
  • The analysis results on NCI-60 data justify the utility of the layered models for the incoming flow of cancer genomic data. (
  • Currently integrative analyses of cancer data focus on three interrelated directions. (
  • Cancer cells usually lack the ability to terminally differentiate. (
  • We compared the expression profiles of NCCIT cells with that of the cancer cell lines in order to identify common genistein-dependent transcriptional changes and accompanying signaling cascades. (
  • We treated primary cancer cells and NCCIT cells with 50 μM genistein for 48 h. (
  • Lung cancer is classified into two major classes as non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). (
  • We measured the growth inhibition elicited by these three agents in a series of 40 human urothelial cancer cell lines and correlated the GI 50 (50% of growth inhibition) values with quantitative measures of global gene expression to derive models of chemosensitivity using a misclassification-penalized posterior approach. (
  • The misclassification-penalized posterior-derived models predicted the growth response of human bladder cancer cell lines to each of the three agents with sensitivities of between 0.93 and 0.96. (
  • Recent studies have indicated that chemotherapeutic sensitivity of the NCI-60 cancer cell line panel can be, in part, predicted by gene expression ( 1 ). (
  • Here, we address this limitation by developing a novel approach that predicts chemotherapeutic responsiveness of human bladder cancer cells to combinations of gemcitabine with cisplatin ( 3 ), cisplatin with paclitaxel ( 4 ), and gemcitabine with paclitaxel ( 5 ) that have shown promising results in clinical trials. (
  • The human bladder cancer cell lines and the respective growth conditions used in this study have been previously described ( 6 , 7 ). (
  • Lung cancer is categorised into small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). (
  • Some of these changes in preneoplastic cell signaling approach those observed in established lung cancer cell lines. (
  • Some of the altered cytokine responses in primary premalignant cells are comparable to those seen in established lung cancer cell lines. (
  • A more recent meta-analysis of family history found that the presence of at least one first-degree relative with melanoma increases the risk by 2.24-fold ( Gandini S, et al, Eur J Cancer, Sep 2005;41(14):2040-59 ). (
  • Validation of a blood protein signature for non-small cell lung cancer. (
  • Ovarian small cell carcinoma of the hypercalcemic type (SCCOHT) is a rare and highly aggressive form of ovarian cancer first reported in 1982 by Dickerson et al. (
  • We reported that horizontal transfer of malignant traits to target cells is a potential pathway to explain cancer dissemination. (
  • In the present study, we exposed BRCA1 -KO fibroblasts to extracellular vesicles (EVs) isolated from a colon cancer cell line (HT29) and from sera of patients with colorectal cancer. (
  • Whole genome sequencing, transcriptome analysis and RNA sequencing of cancer EVs and fibroblasts prior and after exposure to cancer EVs were performed. (
  • Phenotypical transformation of the fibroblasts into colon cancer cells was confirmed by histopathological study of the xenotransplants. (
  • DNA and RNA sequencing suggested that cancer DNA was transferred and possibly transcribed in target cells. (
  • Furthermore, injection of colon cancer EVs in the tail vein of NOD-SCID mice determined neoplastic transformation and metastases in the lungs of the mice confirming for the first time the hypothesis that transfer of malignant epithelial cancer traits to distant target cells is a concept applicable to in vivo models. (
  • Cancer Cell , 13: 496-506. (
  • Detection of prostate-specific antigen- or prostate-specific membrane antigen-constructive circulating cells in prostatic cancer patients: scientific implications. (
  • Random oligonucleotide-primed synthesis (ROPS) revealed a significant increase in uracil misincorporation and DNA single strand breaks, while spectral karyotype analysis (SKY) identified five novel chromosomal rearrangements in cells grown with mild folate depletion. (
  • Stat 3 is known to increase production of a crucial cell cycle protein called cyclin D1. (
  • Mouse embryonic fibroblasts (MEFs) derived from these PRMT3 cells harbor hypomethylated ribosomal protein rpS2 ( 53 ). (
  • In addition, gene ontology analysis indicated G-protein-coupled receptor had a much higher proportion of differential expression (22%) compared with other classes (∼ 5%), suggesting a potential role regulating subtle changes in cellular behavior. (
  • The pathways were functionally confirmed by the FAS ligand-induced cell death and by siRNA knockdown of BAK1 protein. (
  • Western blot analysis showed that Cathepsin D protein was produced at very low levels and was not processed to the mature form. (
  • G) LC3 protein great quantity in charge (Scr) and CerS1 little interfering RNA (siRNA)Ctreated cells incubated with 5 M SoSe for 3 hours. (
  • The important cell cycle regulatory protein cyclin D1 (CCND1) is an essential driver of the first core region of the Chr11q13 amplicon. (
  • CCND1 is a key regulatory protein that plays an important role in the transition from G1 to the S phase of the cell cycle during cell division. (
  • Concomitant with the decrease in H3-Lys9 methylation, telomeres in SUV39DN cells had reduced binding of the chromobox proteins Cbx1, Cbx3 and Cbx5, homologs of Drosophila melanogaster heterochromatin protein 1 (HP1). (
  • Flow microfluorimetry is also used in pharmaceutical research to determine cell type, protein and DNA expression, cell cycle, and other properties of a cell during drug treatment. (
  • 2002). A DNA damage-regulated BRCT-containing protein, TopBP1, is required for cell survival. (
  • 1988). Activation at Mphase of a protein kinase encoded by a starfish homologue of the cell cycle control gene cdc2+. (
  • Inspection of protein markers provides important information about the current state of the cells and data for subsequent manipulations. (
  • The zinc finger CCCTC-binding protein (CTCF) carries out many functions in the cell. (
  • How can a single protein carry out so many different functions in the cell? (
  • NOL7 is a predicted 29 kDa, 257 amino acid protein with no significant homologies to other characterized proteins that localizes to the nucleus and nucleoli of cells. (
  • This paper provides a simple and convenient method to isolate aneuploid cells with complex karyotypes that cease to divide. (
  • What causes normal cells to become aneuploid? (
  • We performed DNA and RNA sequencing of a HeLa Kyoto cell line and analyzed its mutational portfolio and gene expression profile. (
  • The iMyc Eμ -1 cells harbored a reciprocal T(9;11) and three non-reciprocal chromosomal translocations, over-expressed Myc His at the expense of normal Myc , and exhibited gene expression changes on Mouse Lymphochip © microarrays that were consistent with Myc His -driven B-cell neoplasia. (
  • 2005). Activation of PPARγ by curcumin inhibits Moser cell growth and mediates suppression of gene expression of cyclin D1 and EGFR. (
  • We also discuss the capabilities and limitations of the most pertinent techniques for investigating spatial architecture at the population level and in single cells as well as novel approaches that will allow a better evaluation of the relationship between the architecture of the nucleus and features of the linear genome, such as gene expression, histone modification, or binding of transcription factors. (
  • During postdoctoral studies at Virginia Bioinformatics Institute, she developed software (in C, SAS and R) for mass Affymetrix gene-chip data analysis and designed greenhouse and Affymetrix gene expression experiments. (
  • The presence of a monosomal karyotype (MK+) and/or a complex karyotype (CK+) identifies subcategories of AML with poor prognosis. (
  • Technological advances now provide genome-wide and four-dimensional analyses, permitting global characterizations of nuclear order. (
  • During that time, Li applied bi-viariate analysis to improve the power of genome-wide association studies and constructed a Bayesian network using relaxed gene ordering. (
  • Down's syndrome, in which there is trisomy 21, is an example of germ cell aneuploidy. (
  • Importantly, pathway enrichment analysis revealed that these key putative targets were significantly enriched in several RA-related pathways, including cartilage damage-related IL1B-TNF-TLR2-JUN-MMP1-MMP3 signaling pathway. (
  • S. mansoni possesses all the same pyrimidine pathways (de novo, salvage and thymidylate cycles) as those of its host. (
  • Barlow C, Brown KD, Deng CX, Tagle DA, Wynshaw-Boris A (1997) Atm selectively regulates distinct p53-dependent cell-cycle checkpoint and apoptotic pathways. (
  • Proteins extracted from the cells were separated by SDS-PAGE and subjected to Western blot analysis with the indicated antibodies. (
  • After harvesting the cells with RIPA, proteins were separated by SDS-PAGE and subjected to Western blot analysis with anti-p53 (DO-1), -AIMP3, and -tubulin antibodies. (
  • After centrifugation, the proteins in the supernatants were separated by SDS-PAGE and subjected to Western blot analysis or incubated with anti-ATM (Santa Cruz Biotechnology) or anti-ATR (Serotec) antibody for 2 hours for immunoprecipitation. (
  • ES cells were isolated that are homozygous for the PRMT1 hypomorphic allele, and these cells harbor numerous hypomethylated proteins ( 44 ), including Sam68 ( 20 ), MRE11 ( 12 ), histone H4 ( 52 , 61 ), and hnRNPK ( 43 ). (
  • Furthermore, genistein induced the expression of apoptotic and anti-migratory proteins p53 and p38 in all cell lines. (
  • DNA damage is also recognized by ataxia-telangiectasia mutated (ATM) and ATR kinases, which phosphorylate BRCA1, BRCA1-associated proteins and p53 and mediate signalling to form macro-complexes and activate cell cycle checkpoints. (
  • The rapid exchange of proteins between the cytoplasm and the nucleus is a vital process in eukaryotic cells, and this occurs through the nuclear pore complex (NPC), a large macromolecular structure embedded in the double membrane of the nuclear envelope [ 27 - 29 ]. (
  • In 1998, University of Wisconsin researcher Dr. James Thomson succeeded in isolating and culturing human embryonic stem cells for the first time. (
  • WiCell established the first comprehensive training program for human embryonic stem (hES) cells that provided instruction to more than 800 scientists from around the globe during its tenure. (
  • Principal component analysis and K-means cluster analysis of collected Raman spectroscopy data were used for a comparative study of the biochemical fingerprint of human induced pluripotent stem cells and human embryonic stem cells. (
  • Raman spectra analysis revealed that the major difference between human embryonic stem cells and induced pluripotent stem cells is due to the nucleic acid content, as shown by the strong positive peaks at 785, 1098, 1334, 1371, 1484, and 1575 cm -1 , which is enriched in human induced pluripotent stem cells. (
  • Human embryonic stem cells (hESC) offer a renewable source of a wide range of cell types for use in research and cell-based therapies to treat disease. (
  • Human embryonic stem cells (hESC) are a potentially limitless, albeit controversial, source of therapeutic cells for numerous diseases and injuries. (
  • To investigate the impact of H3-Lys9 methylation on telomere length regulation and telomere function, we studied embryonic stem (ES) cells and mouse embryonic fibroblasts (MEFs) from SUV39DN mice 7 , which have less methylation of H3-Lys9 at the pericentric heterochromatin compared to wildtype mice 7 . (
  • However, the phospho-mimic mutant, S224E, is detrimental to mouse embryonic stem cell colonies. (
  • INTRODUCTION The bioactive sphingolipid ceramide is both a structural component of biological membranes and a signaling molecule that induces cell death and tumor suppression (= 3 independent experiments, ** 0.01). (
  • 1994). Anaphase onset in vertebrate somatic cells is controlled by a checkpoint that monitors sister kinetochore attachment to the spindle. (
  • Numerical chromosomal variation is a hallmark of populations of malignant cells. (
  • Subsequently, the cells would undergo the reverse transition, which would allow the reacquisition of their epithelial state (MET) and would enable the malignant cells to escape the circulation and home to the target organs. (
  • In this study, we identify increased STAT3 activation related to dysregulation of the SHP-1 phosphatase and autocrine production of interleukins as a driver of cell survival in breast implant-associated anaplastic large cell lymphomas. (
  • Moreover, dysregulation of this pathway frequently occurs in T cell lymphomas and is implicated in lymphoproliferative autoimmune disease. (
  • In the last five years we have been working on murine T-cell lymphoblastic lymphomas (T-LBLs) arising spontaneously in knockout mice and, in particular, with T-LBLs induced by gamma-irradiation in susceptible and resistant inbred strains, as well as in consomics and congenic derived strains.Results obtained in mouse are confirmed in human cell lines derived from this type of lymphomas and in primary T-LBLs. (
  • Malignancies of the immune system are primarily represented by the malignant lymphomas and a smaller number of nonlymphoid neoplasms that originate from cells involved in antigen presentation and processing. (
  • The STAT3d Mutation Analysis Test detects abnormalities in the STAT3d gene. (
  • What are the Clinical Indications for performing the STAT3d Mutation Analysis Test? (
  • How is the Specimen Collected for STAT3d Mutation Analysis Test? (
  • By contrast, LOH caused by other events, such as UPD, gene conversion, or point mutation would be expected to produce a single monochromatic cell. (
  • Both homologous recombination and nonhomologous end joining (NHEJ) play a role in the repair of DSBs in mammalian cells ( 8 ). (
  • Our findings also suggest that arginine methylation by PRMT1 is a key posttranslational modification in the DNA damage response pathway in proliferating mammalian cells. (
  • 1994). A topoisomerase II-dependent G2 cycle checkpoint in mammalian cells. (
  • Multipolar spindles were found to various degrees in the oral squamous cell carcinoma lines. (
  • Radiosensitivity of CAL 33 cells was intermediate when compared to published data on tumor cell lines. (
  • We report that transient excess of MYC activity can promote tumorigenesis in an immortal rodent cell line and elicit genomic destabilization in both immortal rodent cell lines and in normal human fibroblasts. (
  • Studies of these model cell lines showed strong activation of STAT3 signaling, likely related to autocrine production of IL-6 and decreased SHP-1. (
  • The genetically linked human melanoma cell lines UACC903 and UACC903(+6) phenotypically exhibit distinctive characteristics. (
  • Taxane resistant cell lines exhibited an 18-170 fold increased resistance to taxanes, with the ZR75-1 resistant cell lines also demonstrating cross resistance to anthracyclines. (
  • However, in the resistant cell lines, minimal changes were present. (
  • The studies summarised here characterise taxane resistant cell lines derived by the incremental increase of paclitaxel or docetaxel dose. (
  • Karyotype heterogeneity and clonality were determined by comparing all metaphases within and between the four cell lines by hierarchical clustering. (
  • One of these clusters was characterized by numerical chromosomal abnormalities common to all cell lines, and the other four clusters encompassed cell-specific chromosomal abnormalities. (
  • MCF7 cells showed a chromosomal pattern that was markedly different from those of the other cell lines. (
  • High-performance liquid chromatography analysis demonstrated that mild folate depletion (100 nM) sufficed to induce imbalance in both the nucleotide and AdoMet pools in all prostate cell lines. (
  • These predictions were prospectively evaluated on a series of 15 randomly chosen bladder carcinoma cell lines. (
  • Cell lines were maintained in appropriate media, in a humidified atmosphere containing 5% CO 2 in air, except CRL2169 (SW780), which requires no CO 2 for its growth. (
  • The global leader in cell banking, cytogenetic testing and distribution of stem cell lines, WiCell builds on these core strengths by also providing clinical grade pluripotent stem cell lines, quality control testing and cell banking services. (
  • From 2005 through 2010, WiCell hosted the National Stem Cell Bank, which collected, banked, characterized and distributed the 21 hES cell lines approved for federal funding in the U.S. by then President George W. Bush. (
  • As advancements in technology led to the derivation of human induced pluripotent stem (iPS) cells, WiCell expanded its cell line offerings to include both iPS and modified cell lines, forming the WiCell International Stem Cell (WISC) Bank. (
  • Today, the WISC Bank is home to more than 40 cell lines offered across a variety of culture platforms. (
  • The collection contains cell lines submitted from researchers around the globe and includes the original National Stem Cell Bank lines as well as many modified cell lines useful as research tools. (
  • However, all currently available Prnp −/− lines were generated in embryonic stem cells from the 129 strain of the laboratory mouse and mostly crossed to non-129 strains. (
  • All Prnp −/− lines currently available have been generated in ES cells derived from the 129 strain of the laboratory mouse and are maintained in non-129 backgrounds, with the exception of the Prnp Edbg/Edbg line. (
  • However, cell lines derived after this date are far more numerous, but while it is legal to work on these lines using non-federal funds, information on their properties remains sparse. (
  • The lack of comparative analysis of hESC lines matters, because the properties and behavior of each line are uniquely shaped by their histories. (
  • These results provide the first comprehensive transcriptome analysis of a leukemia stem cell and document an unexpected level of transcriptome variation between phenotypically similar leukemic cells. (
  • Headquartered in Madison, Wisconsin, WiCell is a supporting organization of the University of Wisconsin-Madison, a world leader in the area of human pluripotent stem cell research. (
  • Recognizing the potential of these unique cells, and aware that regulations surrounding their use in a university setting were unclear, the Wisconsin Alumni Research Foundation established WiCell in 1999 as a safe haven for the advancement of stem cell research in the politically charged environment of the time. (
  • As a nonprofit research institute, WiCell has consistently worked to advance stem cell technology through research, education and technical support for the scientific community. (
  • WiCell received the highest rating possible from the NIH at the completion of the NIH National Stem Cell Bank contract. (
  • The mission of WiCell is to support stem cell research at the University of Wisconsin-Madison and the development of stem cell clinical applications worldwide. (
  • Here, we report a nonbiological approach to discriminate human induced pluripotent stem cells from their native embryonic stem cell counterparts. (
  • phase I trial is studying the side effects and best dose of donor mesenchymal stem cells in treating patients with acute or chronic graft-versus-host disease after undergoing a donor stem cell transplant. (
  • Determine the safety of donor mesenchymal stem cell (MSC) infusion in patients with acute or extensive chronic graft-vs-host disease (GVHD) after undergoing HLA-identical sibling donor stem cell transplant. (
  • IL-2 add-back post allogeneic hematopoietic stem cell transplant (HSCT), combined with Sirolimus (SIR), Tacrolimus (TAC) will optimize Treg reconstitution and prevent graft versus host dis. (
  • Low dose Rabbit Antithymocyte Globulin plus Low-dose post-transplantation cyclophosphamide as graft-versus-host disease prophylaxis in haploidentical hematopoietic stem cell transplantatio. (
  • Such dynamic variations are reflected in the genomic heterogeneity among a population of cells, which demands characterization of genomes at the single-cell level ( 4 - 6 ). (
  • NSCLC, which constitutes ~80% of all lung cancers, is further classified into adenocarcinoma, squamous cell carcinoma and large cell carcinoma ( 2 ). (
  • Persistence of Bronchial Dysplasia Is Associated with Development of Invasive Squamous Cell Carcinoma. (
  • Phenotypic characterization showed strong positivity for CD30, CD71, T-cell CD2/5/7, and antigen presentation (HLA-DR, CD80, CD86) markers, and interleukin (IL)-2 (CD25, CD122) and IL-6 receptors. (
  • Here we present a detailed genomic and transcriptomic characterization of a HeLa cell line. (
  • In addition, N-APC cells show enhanced survival following prolonged spindle damage. (
  • Here we investigate roles for the NHEJ factor ARTEMIS in multipotent mesenchymal stem/progenitor cells (MSCs), as putative sarcomagenic cells of origin. (
  • In this context, we postulate a role for Art in suppressing neoplastic transformation of mesenchymal stem/progenitor cells (MSCs), closely related multipotent mesenchymal stromal cells (MMS), or their descendants. (
  • Exhaustion of Airway Basal Progenitor Cells in Early and Established Chronic Obstructive Pulmonary Disease. (
  • These methods have relied on whole-genome amplification (WGA) of an individual cell to generate enough DNA for sequencing ( 17 - 21 ). (
  • The morphological features and the surface marker expression profile of the iMyc Eμ -1 cells were evaluated using cytological methods and FACS, respectively. (
  • Apoptotic mechanisms ensure that cells with damaged DNA that cannot be repaired are destroyed ( 7 ). (
  • Accumulation of DSBs in HCV-infected cells suggests that a HCV-induced oxidative environment may overwhelm cellular antioxidant and DNA repair mechanisms, leading to chromosomal abnormalities. (
  • Adequate cell culture model systems are required for functional studies investigating those potential prognostic markers in HNSCC. (
  • Treatment of iMyc Eμ -1 cells with antibody to CD40 led to the induction of Fas and upregulation of CD45 and CD54, activation markers CD80/86, and CD138, indicating that CD40 signaling was functional ( Additional File 1 ). (
  • However, the functional consequence of COX-2 induction by cannabinoids in these cells has not been established so far. (
  • This increases the numbers of functional transporters at the cell surface thereby raising the V max for glucose entry into the cell ( Klip, McGraw & James, 2019 ). (