Image Cytometry: A technique encompassing morphometry, densitometry, neural networks, and expert systems that has numerous clinical and research applications and is particularly useful in anatomic pathology for the study of malignant lesions. The most common current application of image cytometry is for DNA analysis, followed by quantitation of immunohistochemical staining.Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Cell Cycle: The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.Cytophotometry: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.Ploidies: The degree of replication of the chromosome set in the karyotype.Aneuploidy: The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. In a normally diploid cell (DIPLOIDY) the loss of a chromosome pair is termed nullisomy (symbol: 2N-2), the loss of a single chromosome is MONOSOMY (symbol: 2N-1), the addition of a chromosome pair is tetrasomy (symbol: 2N+2), the addition of a single chromosome is TRISOMY (symbol: 2N+1).DNA, Neoplasm: DNA present in neoplastic tissue.Image Processing, Computer-Assisted: A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.Cell Cycle Proteins: Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Microscopy: The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Cell Line, Tumor: A cell line derived from cultured tumor cells.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Cell Cycle Checkpoints: Regulatory signaling systems that control the progression through the CELL CYCLE. They ensure that the cell has completed, in the correct order and without mistakes, all the processes required to replicate the GENOME and CYTOPLASM, and divide them equally between two daughter cells. If cells sense they have not completed these processes or that the environment does not have the nutrients and growth hormones in place to proceed, then the cells are restrained (or "arrested") until the processes are completed and growth conditions are suitable.G1 Phase: The period of the CELL CYCLE preceding DNA REPLICATION in S PHASE. Subphases of G1 include "competence" (to respond to growth factors), G1a (entry into G1), G1b (progression), and G1c (assembly). Progression through the G1 subphases is effected by limiting growth factors, nutrients, or inhibitors.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).G2 Phase: The period of the CELL CYCLE following DNA synthesis (S PHASE) and preceding M PHASE (cell division phase). The CHROMOSOMES are tetraploid in this point.S Phase: Phase of the CELL CYCLE following G1 and preceding G2 when the entire DNA content of the nucleus is replicated. It is achieved by bidirectional replication at multiple sites along each chromosome.G2 Phase Cell Cycle Checkpoints: CELL CYCLE regulatory signaling systems that are triggered by DNA DAMAGE or lack of nutrients during G2 PHASE. When triggered they restrain cells transitioning from G2 phase to M PHASE.Laser Scanning Cytometry: A scanning microscope-based, cytofluorimetry technique for making fluorescence measurements and topographic analysis on individual cells. Lasers are used to excite fluorochromes in labeled cellular specimens. Fluorescence is detected in multiple discrete wavelengths and the locational data is processed to quantitatively assess APOPTOSIS; PLOIDIES; cell proliferation; GENE EXPRESSION; PROTEIN TRANSPORT; and other cellular processes.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.G0 Phase: A quiescent state of cells during G1 PHASE.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Mitosis: A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

*Cell cycle analysis

... by flow cytometry is an especially useful assay, that has been widely used in analysis of the cell cycle in vitro and in vivo. ... Phoenix Flow Systems, Inc. Retrieved 2010-05-20. "Label-free Cell Cycle Analysis". Phase Holographic Imaging AB. Archived from ... Cell cycle analysis by DNA content measurement is a method that most frequently employs flow cytometry to distinguish cells in ... 3.0.CO;2-N Gray JW, Dolbeare F, Pallavicini MG, Beisker W, Waldman F. (1986) Cell cycle analysis using flow cytometry. Int J ...

*Cell counting

Flow cytometry is by far the most sophisticated and expensive method for cell counting. In a flow cytometer the cells flow in a ... over which a statistical classification algorithm is used to perform automated cell detection and counting as an image analysis ... such as cell-cycle research, they are the method of choice. Its advantage over the methods above is the large number of cells ... for instance the Luria-Delbrück experiment or the gentamicin protection assay). In addition, the enumeration of colonies on ...

*Flow cytometry

Annexin A5 affinity assay, a test for cells undergoing apoptosis, often uses flow cytometry Cell cycle analysis Coulter counter ... A flow cytometer is similar to a microscope, except that, instead of producing an image of the cell, flow cytometry offers high ... Basiji DA, Ortyn WE, Liang L, Venkatachalam V, Morrissey P (2007). "Cellular image analysis and imaging by flow cytometry". ... a flow cell, a measuring system, a detector, an amplification system, and a computer for analysis of the signals. The flow cell ...

*Glutathione

It has roles in progression of the cell cycle, including cell death. GSH levels regulate redox changes to nuclear proteins ... as well as be applied in flow cytometry to perform bulk measurements in high throughput manner. Organelle-targeted RT probe has ... "High-resolution imaging of redox signaling in live cells through an oxidation-sensitive yellow fluorescent protein". Science ... Giustarini D, Dalle-Donne I, Milzani A, Fanti P, Rossi R (September 2013). "Analysis of GSH and GSSG after derivatization with ...

*Fluorescence in situ hybridization

Flow-FISH uses flow cytometry to perform FISH automatically using per-cell fluorescence measurements. Microfluidics-assisted ... or imaged simultaneously with a fluorescent antibody assay. The technology has potential applications in cancer diagnosis, ... which require dividing cells and requires labor and time-intensive manual preparation and analysis of the slides by a ... lncRNA and miRNA in tissues and cells. FISH is used by examining the cellular reproduction cycle, specifically interphase of ...

*Microfluorimetry

... cell cycle analysis and "flow karyotyping" of cells. In flow karotyping, isolated metaphase chromosomes are stained and ... coli bacteriophages lambda and T4 were able to be separated by flow cytometry which allowed for genomic analysis which was ... Limke, TL; Atchison, WD (Nov 2009). "Application of single-cell microfluorimetry to neurotoxicology assays". Current protocols ... an adaption of fluorimetry for studying the biochemical and biophysical properties of cells by using microscopy to image cell ...

*Arabidopsis thaliana

Shaw SL (February 2006). "Imaging the live plant cell". The Plant Journal. 45 (4): 573-98. doi:10.1111/j.1365-313X.2006.02653.x ... using flow cytometry show genome size in Arabidopsis to be approximately 157 Mb and thus approximately 25% larger than the ... Life cycle[edit]. The plant's small size and rapid lifecycle are also advantageous for research. Having specialized as a spring ... A. thaliana is a predominantly self-pollinating plant with an outcrossing rate estimated at less than 0.3%.[84] An analysis of ...

*Carboxyfluorescein succinimidyl ester

"Flow cytometric analysis of cell division by dye dilution". Current Protocols in Cytometry. Chapter 9: Unit 9.11. doi:10.1002/ ... "High-resolution tracking of cell division suggests similar cell cycle kinetics of hematopoietic stem cells stimulated in vitro ... assay to determine susceptibility to lysis by cytotoxic T cells of leukemic precursor cells within a heterogeneous target cell ... Analysis by flow cytometry and fluorescence microscopy". Journal of Immunological Methods. 133 (1): 87-97. doi:10.1016/0022- ...

*Flow cytometry bioinformatics

"Cytometry Standards Continuum" (PDF). SPIE Proceedings. Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues ... "Single-cell mass cytometry adapted to measurements of the cell cycle". Cytometry Part A. 81A (7): 552-566. doi:10.1002/cyto.a. ... correlates of HIV protection can be identified from computational analysis of complex multivariate T-cell flow cytometry assays ... Recently, a GenePattern Flow Cytometry Suite has been developed in order to bring advanced flow cytometry data analysis tools ...

*Reference ranges for blood tests

Determination of monocyte count by hematological analyzers, manual method and flow cytometry in polish population Central ... white blood cells Platelet count (Plt) A few values are for inside red blood cells only: Vitamin B9 (Folic acid/Folate) in red ... Taken from the assay method giving the lowest and highest estimate, respectively, from Table 2 in: Beltran L, Fahie-Wilson MN, ... A separate printable image is available for mass and molarity Smaller, narrower boxes indicate a more tight homeostatic ...

*Survivin

Analysis by flow cytometry revealed an accumulation of cells in S phase upon treatment with resveratrol. The cells were also ... as there is evidence of the presence of cell-cycle-dependent element/cell-cycle gene homology region (CDE/CHR)boxes located in ... A. Cellular T Cell Response The first evidence of survivin-specific CTL recognition and killing was shown in an assay wherein ... as physiological conditions were used to obtain the images. Apoptosis, the process of programmed cell death, involves complex ...

*Arabidopsis thaliana

Chapter 44: Imaging Plant Cells. In: Pawley JB (Editor). Handbook of Biological Confocal Microscopy - 3rd edition. ... Using Flow Cytometry Show Genome Size in Arabidopsis to be 157 Mb and thus 25% Larger than the Arabidopsis Genome Initiative ... 2007). "Complex life cycles of multicellular eukaryotes: new approaches based on the use of model organisms". Gene. 406 (1-2): ... AK Yetisen; L Jiang; J R Cooper; Y Qin; R Palanivelu; Y Zohar (May 2011). "A microsystem-based assay for studying pollen tube ...

*Single-cell variability

... since they will often have a very different cycle than healthy cells. Single-cell analysis of characteristics of the cell cycle ... and could be identified using protocols for staining DNA and measuring its content by flow cytometry or quantitative ... Single-cell gene expression is typically assayed using RNA-seq. After the cell has been isolated, the RNA-seq protocol ... "Single Cell Analysis of Drug Distribution by Intravital Imaging". PLoS ONE. 8 (4): e60988. doi:10.1371/journal.pone.0060988. ...

*Chemical biology

"Microfluidic stochastic confinement enhances analysis of rare cells by isolating cells and creating high density environments ... or fluorescently conjugated antibodies and flow cytometry to provide a detailed, specific, quantitative results with excellent ... To be fully effective, precise imaging systems require a complementary technique that better elucidates the machinery of a cell ... These assays include top agar overlay assays where antibiotics generate zones of growth inhibition against test microbes, and ...

*Assay

... that involve image analysis manually or by software: Cytometry: When the size statistics of cells is assessed by an image ... optic Flow cytometric cell or particle counters, or coulter/impedance principle based cell counters Imaging assays, ... time taken to finish a whole cycle from the preanalytic steps till the end of the last post analytic step (report dispatch/ ... MTT assay Cell Counting Kit-8 (WST-8 based cell viability assay) SRB (Sulforhodamine B) assay CellTiter-Glo® Luminescent Cell ...

*Apoptosis

"Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis)". Cytometry. 27: 1-20. doi:10.1002/( ... leading to cell death. Cell death in organisms is necessary for the normal development of cells and the cell cycle maturation. ... phosphatidylserine exposure versus cell permeability by flow cytometry), cellular markers such as DNA fragmentation (flow ... label-free live cell imaging and quantification". Phase Holographic Imaging AB. Green, Douglas (2011). Means to an End: ...

*Apoptosis

"Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis)". Cytometry. 27: 1-20. doi:10.1002/( ... leading to cell death. Cell death in organisms is necessary for the normal development of cells and the cell cycle maturation.[ ... phosphatidylserine exposure versus cell permeability by flow cytometry), cellular markers such as DNA fragmentation[62] (flow ... "Quantitative phase contrast microscopy - label-free live cell imaging and quantification". Phase Holographic Imaging AB.. ...

*Index of oncology articles

CEA assay - cecum - cefalexin - cefepime - cefixime - ceftriaxone - celecoxib - celiac disease - cell - cell differentiation - ... flow cytometry - floxuridine - flt3L - fluconazole - flucytosine - fludarabine - fludeoxyglucose F 18 - fludrocortisone - ... magnetic resonance imaging - magnetic resonance perfusion imaging - magnetic resonance spectroscopic imaging - magnetic- ... rapid hormone cycling - rapid-onset opioid - ras gene - rasburicase - rattlesnake root - ravuconazole - rebeccamycin - ...

*Apoptosis

"Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis)". Cytometry. 27 (1): 1-20. doi: ... leading to cell death. Cell death in organisms is necessary for the normal development of cells and the cell cycle maturation.[ ... phosphatidylserine exposure versus cell permeability by flow cytometry), cellular markers such as DNA fragmentation[69] (flow ... "Quantitative phase contrast microscopy - label-free live cell imaging and quantification". Phase Holographic Imaging AB.. .mw- ...

*Infection

Enzyme assay. *Mass spectrometry. *Chromatography. *Flow cytometry. *Blood bank. *Microbiological culture. *Serology ... A cell is normally transparent under a microscope, and using a stain increases the contrast of a cell with its background. ... The images are useful in detection of, for example, a bone abscess or a spongiform encephalopathy produced by a prion. ... "Generalized Infectious Cycle" Diagram Illustration". science.education.nih.gov. Archived from the original on 2009-09-24. ...

*Infection

Enzyme assay. *Mass spectrometry. *Chromatography. *Flow cytometry. *Blood bank. *Microbiological culture. *Serology ... A cell is normally transparent under a microscope, and using a stain increases the contrast of a cell with its background. ... The images are useful in detection of, for example, a bone abscess or a spongiform encephalopathy produced by a prion. ... "Generalized Infectious Cycle" Diagram Illustration". science.education.nih.gov. Archived from the original on 2009-09-24. ...

*Hemodynamics

Laminar flow analysis. p. 817. ISBN 978-0470-56510-0.. *^ Marieb, Elaine N.; Hoehn, Katja (2013). "The Cardiovascular System: ... Red cell mass.. RCMH. Cell Mass Available For Transfusion after ANH. RCMI. Red Cell Mass Saved by ANH. SBL. Surgical Blood Loss ... Educational Particle Image Velocimetry (e-PIV) - resources and demonstrations[permanent dead link] ... between the peak systolic velocity and the minimum diastolic velocity divided by the mean velocity during the cardiac cycle. ...

*Chromatography

Enzyme assay. *Mass spectrometry. *Chromatography. *Flow cytometry. *Blood bank. *Microbiological culture. *Serology ... The crude feed, a mixture of soluble proteins, contaminants, cells, and cell debris, is then passed upward through the expanded ... When the sample is treated in the course of an analysis, the phase or the phases containing the analytes of interest is/are ... It was concluded that cycling temperature from 50 degrees to 10 degrees would not be adequate to effectively wash all BSA from ...
The Tali® Image Cytometer is a benchtop assay platform giving you quick, quantitative analysis of GFP/RFP expression, apoptosis, cell viability, and much more. The Tali® Image Cytometer is a 3-channel (bright field, green fluorescence, red fluorescence) benchtop assay platform that performs suspension cell-based assays. Where researchers once relied on
The Tali® Image Cytometer is a benchtop assay platform giving you quick, quantitative analysis of GFP/RFP expression, apoptosis, cell viability, and much more. The Tali® Image Cytometer is a 3-channel (bright field, green fluorescence, red fluorescence) benchtop assay platform that performs suspension cell-based assays. Where researchers once relied on
Nexcelom provides cell counting and image cytometry products including automated cell counters, image cytometers and reagents for your cell counting needs.
Yesterday at the Experimental Biology 2012 conference in San Diego, GE Healthcare unveiled its new bentchtop Cytell Image Cytometer . The device measures u
Image cytometry is generally used for the multiparameter analysis of individual adherent cells, and is used to measure many of the same parameters as flow cytometry. Image cytometry, however, carries the added ability of three-dimensional imaging. High content analysis is a term often applied to image cytometry in the context of high throughput screening, while tissue cytometry refers to the application of these techniques to cells in situ. Image cytometry is generally performed using automated microscopy and computational image processing and analysis ...
The Flow and Image Cytometry Laboratory provides the University of Oklahoma research community with state-of-the-art cell analysis and sorting instrumentation, and the technical expertise to best utilize this technology.. ...
Looking for online definition of DNA ploidy analysis in the Medical Dictionary? DNA ploidy analysis explanation free. What is DNA ploidy analysis? Meaning of DNA ploidy analysis medical term. What does DNA ploidy analysis mean?
The current study found that FEV1% and SDIC were both significantly associated with lung cancer risk. Importantly, the effect of FEV1% was modified by gender such that the impact of reduced lung function on lung cancer risk was greater in men than in women. Regarding prediction, addition of FEV1% and/or SDIC to models did not substantially impact calibration. Addition of FEV1% to the base model significantly improved discrimination as assessed by ROC AUC and by NRI. Addition of SDIC to model 2 led to modest improvement in ROC AUC from 0.767 to 0.773 (P = 0.191). The NRI from risk stratification analysis was 3.1% (P = 0.059). The positive NRI was entirely due to improvement in model specificity-SDIC lead to greater net reclassification of noncases into lower risk categories. Both models 2 and 3 had the same sensitivities in that they classified 123 of 139 cases in the high-risk category (89.2%). However, model 3, compared with model 2, reclassified 3 cases to a lower risk category, whereas ...
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Traditional flow cytometry methods have been remarkably successful at detecting and sorting specific cells in a sample consisting of many cell types. However, a significant limitation of these methods is their inability to be applied in-vivo. Our research focuses on creating and perfecting novel flow cytometry methods which resolve this problem. Previous work includes the development of a two-photon in-vivo flow cytometry system. The use of multiphoton excitation creates an extremely narrow excitation region, making it possible to record signals from single cells as they propagate through the bloodstream. This eliminates the complicated fluid dynamics required for conventional flow cytometry, and also allows the user to focus on a single blood vessel in a ...
TissueGnostics GmbH has added StrataQuest to its line of tissue cytometry solutions. The software suite offers a wide range of choices in image analys
We report the development of a novel flow cytometry-based Ig capture assay (ICA) for the identification and sorting of individual Ab-secreting cells based on their Ag reactivity. The ICA represents a fast and versatile tool for single-cell sorting of peripheral plasmablasts, streamlining subsequent Ab analysis, and cloning. We demonstrate the utility of the assay by isolating Ag-reactive plasmablasts from cryopreserved PBMC obtained from volunteers vaccinated with a recombinant HIV envelope protein. To show the specificity of the ICA, we produced Ag-specific Abs from these cells and subsequently verified their Ag reactivity via ELISA. Furthermore, we used the ICA to track Ag-specific plasmablast responses in HIV-vaccine recipients over a period of 42 d and performed a head-to-head comparison with a conventional B cell ELISpot. Results were highly comparable, highlighting ...
There is a constant need of features able to characterize potentially metastatic cells among the heterogeneous cell subpopulations which constitute a tumor. Image cytometry of metastatic tumor cells give rise to variable results, partly because of a heterogeneous origin of cells, or potential drug effects. The aim of this work was to characterize nuclear changes observed in metastatic cell clones issued in vitro from the same parental cell population The nuclear phenotypes of 6 cell sublines isolated from a rat rhabdomyosarcoma cell line and differing in their metastatic ability were evaluated by image cytometry on Feulgen-stained preparations. Densitometric [5], geometric [3] and textural [9] features were computed from each nuclear image. For each ...
Commercial organizations. Regional Segments:. Regional segmentation details the regional aspects of the global Flow Cytometry market. and explains the restrictive framework thats probably to impact the market. It highlights the political scenario in the market and the anticipates its influence on the global Flow Cytometry market.. • Middle East and Africa (Egypt and GCC Countries). • North America (United States, Mexico, and Canada). • South America (Brazil etc.). • Europe (Germany, Turkey, Russia UK, Italy, France, etc.). Get UPTO 25% OFF On Flow Cytometry Market Report (Valid Till 15Jan 2020). Inquire/Speak To Expert for Further Detailed Information About Flow Cytometry Report: https://marketresearch.biz/report/flow-cytometry-market/#inquiry. Table of ...
TY - JOUR. T1 - The prognostic value of multiparametric flow cytometry in AL amyloidosis at diagnosis and at the end of first-line treatment. AU - Muchtar, Eli. AU - Jevremovic, Dragan. AU - Dispenzieri, Angela. AU - Dingli, David M. AU - Buadi, Francis K.. AU - Lacy, Martha. AU - Gonsalves, Wilson. AU - Hayman, Suzanne R.. AU - Kapoor, Prashant. AU - Leung, Nelson. AU - Russell, Stephen J. AU - Lust, John A.. AU - Lin, Yi. AU - Go, Ronald S.. AU - Chakraborty, Rajshekhar. AU - Zeldenrust, Steven. AU - Kumar, Shaji K. AU - Kyle, Robert A.. AU - Rajkumar, S Vincent. AU - Gertz, Morie. PY - 2017/1/5. Y1 - 2017/1/5. N2 - Multiparametric flow cytometry (MFC) in amyloid light-chain (AL) amyloidosis has not been widely adopted and, consequently, there is little information on its clinical relevance. We studied 173 patients with AL amyloidosis who underwent MFC immunophenotyping of bone marrow sample at diagnosis ...
Flow cytometry is conventionally used to measure cell-surface antigen expression. However, many antigens are found within the cytoplasm, and it is necessary to fix and permeabilize cells to enable antibodies to gain access to them. In this study we have established the conditions for studying intracellular antigens in human trophoblast cells by flow cytometry using an antibody to TAP1 (a key molecule in the process of Class I MHC assembly). We have previously shown by immunocytochemistry that TAP1 expression is apparently greater on Class 1 positive extravillous cytotrophoblast than on any other fetal or maternal tissue. However, as immunohistochemistry is not quantitative we have used three-colour flow cytometry to measure the expression of TAP1 in different trophoblast populations. Villous ...
Presenter: Leo L. Chan, Nexcelom Bioscience, Lawrence, MA Coauthors: Daniel J. Laverty, Alexandria Kury, Dmitry Kuksin, Alnoor Pirani, and Kevin Flanagan, Nexcelom Bioscience, Lawrence, MA. Concentration, viability, and budding percentages of Saccharomyces cerevisiae are routinely measured in the biofuel and brewing industries. Measurement of these parameters is of great importance in a manufacturing setting because they can aid in the estimation of the product quality, quantity, and fermentation time of the manufacturing process. Specifically, budding percentages can be used to estimate the reproduction rate of yeast populations, which directly correlates with metabolism of polysaccharides and bioethanol production, and can be monitored to maximize production of bioethanol during fermentation. The traditional method involves manual counting using a hemacytometer, but this is time-consuming and prone to human error. In this study, we developed a novel automated method for the quantification of ...
Central Department of Biotechnology, Tribhuvan University is organizing International Workshop on Applications of FLOW CYTOMETRY on Biotechnology -2017 on September 14-17, 2017.. For detail information, please click the linked below:. Download Application form and send the filled application form to This email address is being protected from spambots. You need JavaScript enabled to view it.. or submit to Central Department of Biotechnology, Tribhuvan University, Kirtipur. Detail Information. ...
Hi Philipp, You can get devel versions of R at ftp://ftp.stat.math.ethz.ch/Software/R/. I believe the idea has always been that BioC devel (i.e., 2.6) works with R devel; iFlow is in BioC 2.6 so that should work. However, iFlow may not necessarily need the latest R. I havent tried the latest version but I have been playing with iFlow back in 2009 using R 2.9 or so. You can install iFlow from sources from the SVN repository (https://hedgehog.fhcrc.org/bioconductor/trunk/madman/Rpacks) user readonly, password readonly. To install it, run the R CMD INSTALL ,path to your iFlow,. The only pain with this approach is that you will have to manually resolve all the dependencies. Good luck. Cheers, Josef , Date: Mon, 08 Feb 2010 11:52:41 +0100 , From: Philipp Meng ,philipp.meng at meduniwien.ac.at, , To: Martin Morgan ,mtmorgan at fhcrc.org, , Cc: bioconductor ,bioconductor at stat.math.ethz.ch, , Subject: Re: [BioC] Installing ...
Background: Invariant natural killer cells (iNKT) are an important immunoregulatory T cell subset. Currently several flow cytometry-based approaches exist for the identifi-cation of iNKT cells, which rely on using the 6B11 monoclonal antibody or a combina-tion of anti-Vα24 and anti-Vβ11 antibodies. Objective: The aim of this study was to compare the ability of two flow cytometry-based methods for detecting the frequency of circulating iNKT cells. Methods: The frequency of iNKT cells was detected in the pe-ripheral blood of 37 healthy adult donors by flow cytometry using the 6B11 antibody or a combination of anti-Vα24 and anti-Vβ11 antibodies. Results: The frequency of iNKT cells detected by 6B11 antibody or by combination of anti-Vα24 and anti-Vβ11 ...
Some snippets from your converted document: Designing and Implementing a High-Level Multicolor Flow Cytometry Assay Brent Wood MD PhD Department of Laboratory Medicine University of Washington Define Purpose of Assay • Most important question - What information is required? - What information is most important? • Prioritize • Compromises are inevitable - Simplest assay is best Outline • Instrument optimization • Compensation • Reagent selection and optimization • Panel validation • Data analysis Detector Optimization Detector Optimization PMT Voltage Green = 400 V, Blue = 450 V, Red = 500 V, Violet = 550 V Detector Voltage 475 volts 575 volts 675 volts 775 volts 875 volts • Too low reduces sensitivity • Too high pushes bright signals off scale - Dilute with unlabeled antibody • May need to compromise Optimization • Daily optimization not practical • Once optimized, do NOT change instrument settings unless change in performance - ...
Background: The neutral comet assay was devised to measure double-stranded DNA breaks, but it has also been used to measure apoptosis based on its characteristic DNA fragmentation patterns. There is still uncertainty about the reliability of this method. By comparing the comet assay with a flow cytometry method that uses Annexin V binding to apoptotic cells, we have provided further evidence for evaluating the usefulness of the comet assay for detecting apoptosis. Methods: Apoptosis was induced in human peripheral blood mononuclear cells (PBMC) by ionizing radiation and measured using the comet assay and a flow cytometry method that measures Annexin V and propidium iodide (PI) staining. Results: The Annexin V flow cytometry assay distinguished among early apoptosis, late apoptosis, and an apoptotic or necrotic phase in which ...
TY - JOUR. T1 - Polychromatic flow cytometry analysis of CD34+ hematopoietic stem cells in cryopreserved early preterm human cord blood samples. AU - D'Alessio, F.. AU - Mirabelli, P.. AU - Gorrese, M.. AU - Scalia, G.. AU - Gemei, M.. AU - Mariotti, E.. AU - Di Noto, R.. AU - Martinelli, P.. AU - Fortunato, G.. AU - Paladini, D.. AU - Del Vecchio, L.. PY - 2011/1. Y1 - 2011/1. N2 - During the last decades, extended characterizations were performed of human full-term cord blood (hTCB) cells, but little information is available on human early preterm cord blood (hEPCB) hematopoietic stem cells (HSCs). In our study, we analyzed by flow cytometry 19 hEPCB and 17 hTCB samples. First, we observed that the percentage of CD34 PosCD45 Dim cells was higher in hEPCB compared with hTCB and that it decreased during 16th-20th week ...
The CellROX Orange Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX Orange Reagent, as well as SYTOX Red Dead Cell Stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-b
Flow cytometry immunophenotyping is invaluable for the diagnostic and prognostic work-up of haematological malignancies. Over the last decade, multi-colour flow cytometry analysis has been used increasingly to investigate leptomeningeal disease; several studies show the utility and sensitivity of this technique for both B cell Non-Hodgkins Lymphoma and Acute Leukaemia diagnostics. Cells within CSF samples are typically low in number and degrade quickly. Therefore, the recommendation is to use cell stabilisation reagents for CSF samples that require flow cytometry analysis. Previous studies have shown that TransFix stabilised CSF samples have a cell yield equal to or better than that of fresh CSF samples and so may be used for immunophenotypic ...
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p style="margin: 0cm 0cm 0pt; text-align: justify; line-height: normal;",Introduction: Medicinal plants are considered to be safer, non-toxic and less harmful as compared to synthetic based drugs that are available. In this study, we focused on aqueous leaves extract of Calotropis gigantea for determining its antimicrobial activity in infected (dengue) human whole blood samples using flow cytometry.Methods: Infected dengue human blood samples (n = 5; confirmed on the basis of NS1 antigen to dengue virus;) were collected from pathology lab and evaluated its blood counts (lymphocytes, monocytes and granulocytes count); forward scatter (FSC) and side scatter (SSC) including CD14 monocyte surface marker following the use of variable doses of aqueous leaves extract of C. gigantea.Results: In this study, the results showed that aqueous leaves extract of C. gigantea caused enhancement in case of granulocytes FSC (shape and size) and SSC (granularity) counts but ...
Objective To investigate the prognostic significance of DNA ploidy and Ki67 expression in eyelid basial cell carcinoma. Methods A total of 23 cases of eyelid basal cell carcinoma were studied for the expression of Ki67 by immunohistochemistry, and DNA ploidy determined by image cytometer(ICM). Results Of the 23 cases of eyelid basal cell carcinoma, DNA aneuploidy was seen in 15 cases (65. 2%), presenting as 90% in recurrent basal cell carcinoma following treatment but it was only 37. 5% in nonrecurrent cases, this difference was statistically significant (P = 0. 043). The labeling indexes of Ki67 expression were 0. 272±0. 134 in recurrent tumors vs 0. 197±0. 019 in nonrecurrent ones. There was a notable increase in the percentage positive for Ki67 in recurrent tumors as comparing with the nonrecurrent tumours, showing no statistical significance (P = 0. 069). Conclusions Aneuploidy in eyelid basial cell ...
TY - CHAP. T1 - Analysis of Plant Gene Expression Using Flow Cytometry and Sorting. AU - Galbraith, David W. PY - 2007/5/21. Y1 - 2007/5/21. KW - Analysis of plant gene expression. KW - Combining flow and image cytometry. KW - Defining cellular states. KW - Flow cytometry. KW - Flow sorting. KW - Methods. KW - Technologies. KW - Use of protoplasts for confirmatory studies. UR - http://www.scopus.com/inward/record.url?scp=46449131685&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=46449131685&partnerID=8YFLogxK. U2 - 10.1002/9783527610921.ch17. DO - 10.1002/9783527610921.ch17. M3 - Chapter. AN - SCOPUS:46449131685. SN - 9783527314874. SP - 405. EP - 422. BT - Flow Cytometry with Plant ...
Background: C reactive protein (CRP), an important serum marker of atherosclerotic vascular disease, has recently been reported to be active inside human atherosclerotic plaques.. Aims: To investigate the simultaneous presence of macrophages, CRP, membrane attack complex C5b-9 (MAC), and oxidised low density lipoprotein (oxLDL) in atherectomy specimens from patients with different coronary syndromes.. Methods: In total, 54 patients with stable angina (SA; n = 21), unstable angina (UA; n = 15), and myocardial infarction (MI; n = 18) underwent directional coronary atherectomy for coronary lesions. Cryostat sections of atherosclerotic plaques were immunohistochemically stained with monoclonal antibodies: anti-CD68 (macrophages), anti-5G4 (CRP), aE11 (MAC), and 12E7 (oxLDL). Immunopositive areas were evaluated in relation to fibrous and neointima tissues, atheroma, and media. Quantitative analysis was performed using image cytometry with ...
Reliable autofocus is required to obtain accurate measurements of fluorescent stained cellular components from a system capable of scanning multiple microscope fields. Autofocus could be performed directly with fluorescence images, but due to photobleaching and destructive fluorescence by-products, it is best to minimize fluorescence exposure for photosensitive specimens and live cells. This exposure problem could be completely avoided by using phase-contrast microscopy, implemented through the same optics as fluorescence microscopy. Functions for both phase-contrast and fluorescence autofocus were evaluated using the present invention and the suitability of phase-contrast autofocus for fluorescence microscopy was determined. The present autofocus system for scanning microscopy can be performed at least as fast as 0.25 s/field without loss of precision. The speed of autofocus can be further increased by a volume image which is obtained by ...
The cytokinesis-block micronucleus (CBMN) assay is employed in biological dosimetry to determine the dose of radiation to an exposed individual from the frequency of micronuclei (MN) in binucleated lymphocyte cells. The method has been partially automated for the use in mass casualty events, but it would be advantageous to further automate the method for increased throughput. Recently, automated image analysis has been successfully applied to the traditional, slide-scoring-based method of the CBMN assay. However, with the development of new technologies such as the imaging flow cytometer, it is now possible to adapt this microscope-based assay to an automated imaging flow cytometry method. The Image StreamX is an imaging flow cytometer that has adequate sensitivity to quantify radiation doses larger than 1 Gy while adding the increased throughput of ...
Figure 5. ExoFlow-ONE dyes multiplexed with antibody-conjugated dyes are able to identify subpopulations of EVs. Murine-derived EVs were isolated and co-stained with Emerald Green ExoFlow-ONE and an APC-conjugated antibody against a cell adhesion protein (A) or Emerald Green ExoFlow-ONE and a PE-conjugated antibody against a macrophage membrane protein (B). In panel A, right, two distinct populations of EVs can be seen (Emerald Green axis), each of which expresses a range of the cell adhesion protein (APC axis). In contrast, in panel B, right, while the two distinct populations of EVs can still be seen (Emerald Green axis) the EVs do not appear to express the macrophage-specific protein. Thus, ExoFlow-ONE dyes multiplexed with antibody-conjugated dyes can be used to easily detect subpopulations of EVs. ...
Purpose: Mantle cell lymphoma (MCL) is a mature B-cell lymphoma considered to be incurable with current treatments including: front-line rituximab in combination with multi-agent chemotherapy and for those eligible, high dose-chemotherapy and stem cell support or rituximab maintenance. On the other hand, achieving a complete remission by high-sensitive flow cytometry is associated with prolonged duration of remission, stressing the need to develop and/or incorporate novel agents into the management of MCL. To this end, we examined the activity of ofatumumab, an anti-CD20 monoclonal antibody with distinct binding and immunological properties than rituximab, in MCL pre-clinical models. Experimental Design: MCL cells were labeled with 51Cr prior to incubation with rituximab or ofatumumab (10 µg/ml) plus human serum or effector cells. 51Cr-release was measured and the percentage ...
Autofocus is a critical aspect of any automated microscopy application. Here we report on the successful exploitation of chromatic aberration to speed autofocus for biological microscopy. Using pairs of lenses aligned nominally as infinite conjugates, the chromatic aberration produced by a microscope can be manipulated to achieve multiplanar imaging covering a wide range of spacings. Using both three-chip CCD and Bayer-array color cameras, chromatic aberration-based multiplanar imaging is used to reduce by a factor of 3 the number of mechanical movements of the biological sample necessary to determine best focus. Chromatic aberration-based autofocus is validated using a ...
G.Valet: personalized medicine,individualized medicine: 1.Predictive Medicine by Cytomics, 2.Human Cytome Project & Concepts in Cytomics, 3.Medical Bioinformatics (Data Pattern Classification), 4.Cell Function in Cytomics, 5.CytoRelay, 6. Archive Cell Biochemistry Max-Planck-Institut f r Biochemie, Martinsried, Germany
Background: DNA ploidy analysis of cervical intraepithelial neoplasia (CIN) and invasive cervical cancer samples by flow cytometry (FCM) has been established as an aid to prognostic assessment. Liquid based cytology (LBC) increases diagnostic specificity by using ancillary techniques that provide information beyond morphology. The present study was undertaken to assess DNA ploidy in LBC samples as an adjunct for early detection of cervical pre-cancer and cancer. Methods: DNA ploidy assessment was performed on LBC samples of 50 cases and 31 controls. Cell pellets were obtained by centrifugation and stained with Telford reagent. At least 20,000 R1 gate (G0-G1) events were acquired on a BD FACSCalibur by using a 575±10 nm filter. Results: Mean diploid G1 values were lowered significantly (p|0.01) while diploid S values were significantly elevated (p|0.01) in both high grade squamous intraepithelial lesions (HSILs) and squamous ...
Beckman Coulter Life Sciences has received 510(k) clearance from the U.S. Food and Drug Administration and an Import Medical Device Registration Certificate from the China Food and Drug Administration for the in vitro diagnostic use of the Navios flow cytometry system. In the U.S., the Navios flow cytometer is intended for immunophenotyping in conjunction with proprietary Navios tetra software and CYTO-STAT tetraCHROME reagents.
0050] The embodiment of FIG. 8 also shows that the bit line voltage can be different for each layer, depending on the programming rate of that particular layer. The bit line voltages are different in order to maintain the same voltage delta between the bit line and the source line for each layer. Thus, assuming the voltages of FIG. 8 are used, the bit line BL3 for the top, slowest layer 703 is biased at 1.1V. The bit line BL2 for the next fastest layer 702 is biased at 0.9V. The bit line BL1 for the next fastest layer 701 is biased at 0.7V. The bit line BL0 for the fastest layer 700 is biased at 0.5V. These different bit line voltages maintain the same voltage delta (e.g., 0.5V) for each of the layers. In the illustrated embodiment, the slower the programming of the layer 700-703 to which the bit line is coupled, the higher the bit line voltage ...
0050] For example, in the two-dimensional coordinate system shown in FIGS. 9A and 9B, it is assumed that a first current source exists at a position of (x, y)=(0, 2) (that is, the position where the distance in the y-axis direction is 2). The magnitude of a current outputted from the first current source is 1, and the direction of the current is the -z-axis direction (direction directed from the back to the front of the paper plane). If only the first current source exists, the measured value becomes a waveform s1 when the magnetocardiogram of the subject P is measured from the front surface. On the other hand, it is assumed that a second current source exists at a position of (x, y)=(0, 4) in the two-dimensional coordinate system shown in FIGS. 9A and 9B (that is, the position where the distance in the y-axis direction is 4) in addition to the first current source. The magnitude of a current outputted from the second current source is 0.3, and the direction of the current is the z-axis ...
A semiconductor memory device is rescued from a defective product by replacing a column of regular memory cells containing a defective memory cell with a column of redundant memory cells, and allows a multi-bit data code to be read out partially from regular memory cell arrays as well as partially from the column of the redundant memory cells by permanently disabling a regular column address decoder circuit associated with the column of the regular memory cells replaced with the column of the redundant memory cells so that the other memory cell arrays and the column of the redundant memory cells become concurrently accessible.
As part of our ongoing commitment to support our customers and aid in their success, weve opened a Fluidigm customer inspiration center in Markham, Ontario, the area of Greater Toronto that is Canadas tech capital and the birthplace of mass cytometry. We developed this functional facility to provide customers with a firsthand view of CyTOF® technology on Helios™, the most advanced mass cytometry system ever built. "Our new mass cytometry customer inspiration center was developed in response to increasing interest from our customers," said Dmitry Bandura, Vice President of Research and Development at Fluidigm. "While our new center will initially enable demonstrations of mass cytometry, we plan to expand this center in the future to include training courses to certify new users on best practices using this transformational technology." Mass ...
FIDIS (BMD, France) is a multiplex analytical flow cytometry system for the detection of antibodies. The aim of this study was to evaluate the FIDIS connective assay system for the detection of antinuclear antibodies (ANA), and to assess the clinical utility of these parameters in the diagnosis of connective tissue diseases. The FIDIS system simultaneously measures IgG antibodies directed at dsDNA, Ro, La, RNP, Sm, Jo-1, Scl-70, rRNP, and CENP-B. ...
Most human cancers have abnormal DNA content, having lost some chromosomal parts and gained others. The present study provides some evidence as to the effect of these gains and losses on gene expression in two pairs of non-invasive and invasive TCCs using high throughput expression arrays and proteomics, in combination with CGH. In general, the results showed that there is a clear individual regulation of the mRNA expression of single genes, which in some cases was superimposed by a DNA copy number effect. In most cases, genes located in chromosomal areas with gains often exhibited increased mRNA expression, whereas areas showing losses showed either no change or a reduced mRNA expression. The latter might be because of the fact that losses most often are restricted to loss of one allele, and the cut-off point for detection of expression alterations was a 2-fold change, thus being at the border of detection. In several cases, however, an increase or decrease in DNA copy number was associated ...
Structural variation in the human genome is likely to be an important mechanism for neuronal diversity and brain disease. A combination of multiple different forms of aneuploid cells due to loss or gain of whole chromosomes giving rise to cellular diversity at the genomic level have been described in neurons of the normal and diseased adult human brain. Here, we describe recent advances in molecular neuropathology based on the combination of slide-based cytometry with molecular biological techniques that will contribute to the understanding of genetic neuronal heterogeneity in the CNS and its potential impact on Alzheimer´s disease and age-related disorders.
A disease and a drug interfere with multiple cellular function(s) and the impact on the cell differs depending on the cell type and its functional status (e.g. differentiation, cell cycle, diurnal oscillations). In recent years, cells have become increasingly studied as an instrument to treat complex diseases as in gene therapy and stem cells research. We need to improve our understanding of the dynamics of cellular behavior in order to improve our chances for success (7).. Screening of functional targets in drug discovery requires the appropriate intracellular environment to be present. The proper function of an intracellular protein depends on its processing and environment. Because of the heterogeneity of cell types and structural and functional differences between cells (e.g. hepatocytes, neural ...
MCF-10F human breast epithelial cells when transformed with 17-beta-estradiol (E2) give rise to highly invasive C5 cells that generate adenocarcinomas in SCID mice. From these tumor, cell lines Such as C5-A6-T6 and C5-A8-T8 have been derived. Variable patterns of chromatin supraorganization have been demonstrated for these cells during the transformation/tumorigenesis progress, when assessing chromatin entropy by image analysis in Feulgenstained preparations. Since epigenetic dysregulation might contribute to the chromatin textural repatterning in transformed MCF-10F cells, the association of the variable chromatin packing states with global DNA methylation was investigated in these cells after their treatment with restriction enzymes followed by Feulgen staining and chromatin entropy evaluation by image analysis. The results indicate that ...
In cell signaling, different perturbations lead to different responses and using traditional biological techniques that result in averaged data may obscure important cell-to-cell variations. The aim of this study was to develop and evaluate a four-inlet microfluidic system that enables single-cell analysis by investigating the effect on Hog1 localization post a selective Hog1 inhibitor treatment during osmotic stress. Optical tweezers was used to position yeast cells in an array of desired size and density inside the microfluidic system. By changing the flow rates through the inlet channels, controlled and rapid introduction of two different perturbations over the cell array was enabled. The placement of the cells was determined by diffusion rates flow simulations. The system was evaluated by monitoring the subcellular ...
DESCRIPTION (provided by applicant): Cell Microsystems is a North Carolina-based start-up biotechnology company whose mission is to commercialize a novel, yet affordable, platform for the efficient isolation of viable, single cells or colonies from a mixedpopulation while the cell/colony remains adherent or encapsulated to a solid surface. The companys IsoRaft technology is based on a unique cell array recently developed at the University of North Carolina (UNC) at Chapel Hill, and represents an ideal opportunity for the translation of an academic technology to the marketplace through the SBIR program. Cell Microsystems has obtained license from UNC to commercialize the technology for a broad market in academic labs, as well as in the biotechnology and pharmaceutical industries. The products consist of disposable microarray (the IsoRaft Array) for culturing cells and a simple device for isolating the ...
The primate retina provides the sole input to central visual mechanisms, through a well defined set of receptors and cell arrays. We have investigated how information is distributed among these arrays when natural temporal stimuli are presented to the retina. Chromatic information in the PC channel is confined to low temporal frequencies (Fig. 10). Even for the particularly colorful stimuli used here, this channel carries approximately five times less information in the chromatic than in the achromatic domain (Table 3). Information in the S-cone-driven ganglion cells is also confined to low temporal frequencies. Information in the MC pathway extends to higher frequencies than the achromatic component of the PC channel signal, but the MC pathway transmits less signal power than in the PC channel for frequencies below 2-3 Hz (Fig. 11).. PC cells show much greater cone contrast sensitivity to chromatic than to luminance modulation (Lee et al., 1993). The ...
Content-Type: text/plain; charset=us-ascii > >Hi Yan Gao, > >I think you should contact Zymed. They have very high >quality Tissue Microarray, especially the human ones. >800-874-4494 > >Scott >CSU > >-----Original Message----- >From: [email protected] >[mailto:[email protected]]On >Behalf Of yan gao >Sent: Thursday, April 29, 2004 7:11 PM >To: [email protected] >Subject: [Histonet] Tissue array > > > > Hi, Histonet. > I am interested to get a few tissue array for >different human cancers > and cell array from human tumor cell lines. Any >recommendation? > > Yan Gao > Ph.D > Norvatis > >_________________________________________________________________ >Is your PC infected? Get a FREE online computer virus scan from >McAfee Security. >http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 > > >_______________________________________________ >Histonet mailing list >[email protected] ...
We focus on the development of low-cost and simple impalefection systems that can be easily integrated in the workflow of cell biology laboratories as an alternative to traditional reverse transfection cell arrays.. ...
The size of the cells and the cell array varies a lot in different implementations. A usual implementation will have either 8bit or 32bit cells with 30000 cells (in the positive direction). For Turing completeness either the number of cells must be unbounded or (at least) three unbounded cells are required, the former is usually assumed. Urban Müllers compiler used an array of 30000 cells 8bit cells, while the interpreter only allowed 100 (of 5000) to be used. As the compiler was written in assembler there is no indication as to whether the cells are to be assumed to be signed or unsigned and the overflow semantics are of the usual twos complement (or unsigned byte) wrapping form. The interpreter uses signed 8bit characters (-128 to 127 range). Other interpreters normally use a similar layout, however, some allow cells to ...
Flow cytometer analysis. Researcher loading cell culture samples into a flow cytometer, a machine that will analyse the cultures and measure a range of parameters. The samples are human cell cultures, being analysed as part of research into induced pluripotent stem cell (iPSC) lines. The research looks at human cell responses to drugs, supporting the development of new drugs. This research is being carried out at Roslin Cell Sciences, Roslin BioCentre, Edinburgh, Scotland. Roslin Cell Sciences was created in 2016 as a subsidiary of Roslin Cells. Photographed in 2016. - Stock Image C035/6624
Flow cytometry (or FACS) is a technology that allows the rapid measurement of fluorescently labelled samples and common applications include cell cycle, ploidy analysis, proliferation, apoptosis, immunophenotyping, live/dead assays and expression analysis. This technology can also be used to separate out individual cells to either improve transfection efficiencies or create stable cell lines. The facility is located on Level 5 (5.13) of the L.C.Miall building. Please follow the links on the left hand side to find out more about the facility, the equipment available and how it can help your research.. Upcoming course. In conjunction with InCytometry (www.incytometry.co.uk) the facility will be re-running the one day course on the Basics of Flow ...
A quick fix for this is to put a fresh tube of water on the SIP and start running the system while you set up the electronics - and this issue will be avoided. As a side note, there are some other fluidics arrangements out there - such as the Attune, which uses an acoustic wave to focus the cells in the center of the core stream, and the Guava instruments, which use a microfluidics capillary system, meaning no separate sheath fluid.. These types of issues can also arise on these systems, so watch your data.. Understanding the fluidics system and observing the consequences of the system during acquisition is important to solve problems before they become major issues. While walk-away options exist on cytometers (such as high throughput sampling systems), care must be taken to make sure that the samples are properly prepared so that clogs and other preventable nuisances are avoided and data is not lost.. To learn more about how to troubleshoot the flow cytometer fluidics ...
A liquid flow cytometer for counting and classification of particles such as biological cells and other microscopic particles in liquids that involves an optical system for irradiation and detection in a metering zone (7), thus enabling optical dispersion and fluorescence caused by the particles to be simultaneously detected when the particles pass the metering zone, includes in a first beam path (S1) a mirror reflector (9) with its focal point in the metering zone (7), a quarter-wave plate (8) between the metering zone (7) and the mirror reflector (9), a lens (6) with its focal point in the metering zone (7) and located on the opposite side of it together with a dichroic mirror (5) behind the lens and provided in such a manner that it gives reflection to a second beam path (S2). In the second beam path (S2) a beam splitter (4) is provided between the dichroic mirror (9) and a known per se light source (1), and produces a third beam path (S3). The cytometer includes a first ...
Gillings - Mathematics in the download signal theory methods in multispectral of the breweries, New York, Dover Publications,1982- David Reimer - Count like an 3D. A future download signal to Last beers, Princeton( NJ)- Oxford, Princeton University Press, 2014- Corinna Rossi - Architecture and Mathematics in Ancient Egypt, Cambridge- New York, Cambridge University Press, 2007- Sarah Symons - small metaphysical frequency. Praha, Nakladatelstwvi Ceskoslovenske Akademie Ved, 1953- Richard A. Parker - The Calendars of Ancient Egypt, Chicago, The University of Chicago Press, 1950- Anthony John Spalinger, download signal theory methods in multispectral remote sensing. examples in Time: corners in Ancient Egyptian Calendrics, San Antonio( TX), Van Siclen Books, 1994- Jack Lindsey - The attractive download signal theory in Graeco-Roman Egypt - London, Frederick Muller, 1970BIOLOGY, ZOOLOGY AND BOTANICS- Lise Manniche- An Ancient Egyptian Herbal, Austin, University of Texas Press, 1993- F. Flowers ...
Hybrid diamondlike carbon (h-DLC) coatings for multispectral use combine the hardness of protective DLC coatings with the multispectral functionality
On the streets, it is not about capturing nice colours or aesthetically pleasing shots all the time. It is about capturing emotions, expressions of people passing by in that small trace of time, and moments that happen so briefly that you capture that one frame to tell a wonderful story. Being able to capture the decisive moment (as popularized by Henri Cartier Bresson, and no I am not a fan of his) will demand a lot from the camera, when it comes to Autofocus. Having very fast, accurate and reliable autofocus in the E-M5 allowed me to nail my shots at that precise moment I wanted the shutter to click. I know many people rely on zone-focusing (lets hope this statement wont spark a whole new firestorm elsewhere), personally I prefer NOT to use zone focusing because I want bokeh and I use high shutter speed on the streets, hence good Autofocus is a necessity for me. Of course you may devise your own shooting style that fits you, but you have to admit that being able to focus and freeze the moment ...
TY - JOUR. T1 - Method for counting mitoses by image processing in Feulgen stained breast cancer sections. AU - ten Kate, T K. AU - Beliën, J A. AU - Smeulders, A W. AU - Baak, J P. PY - 1993. Y1 - 1993. N2 - This study describes an image processing method for the assessment of the mitotic count in Feulgen-stained breast cancer sections. The segmentation procedure was optimized to eliminate 95-98% of the nonmitoses, whereas 11% of the mitoses did not survive the segmentation procedure. Contour features and optical density measurements of the remaining objects were computed to allow for classification. Twelve specimens were analyzed, nine used to serve as a training set, and three put aside for later use as independent test set. The fully automatic image processing method correctly classified 81% of the mitoses at the specimen level while inserting 30% false positives. The automatic procedure strongly correlated with the interactive counting procedure (r = ...
Medica Corporation (Bedford, Mass.) is globally launching the EasyCell assistant cell image analyzer for hematology laboratories. The EasyCell scans blood
... ,Ultracompact high end desktop multilaser flow cytometer for all applications in Cytomics and any kind of cell analysis and absolute counting. The CyFlow ML allows to analyze forward and two different side scatter signals in combination with up to 13 fluorescence channels. The new CyFlow ML instrume,medicine,medical supply,medical supplies,medical product
Find and save ideas about Plant cell on Pinterest. | See more ideas about Plant cell images, Plant cell structure and Biology art.
This paper reports a light sheet fluorescence imaging flow cytometer for 3D sectioning of phytoplankton. The instrument developed has the inherent advantages of high cell counting throughput and high spatial resolution information derived from flow cytometry and light sheet microscopy. The throughput of the instrument is quantified by the sample volume flow rate of 0.5 μl/min with a spatial resolution as achieved by light sheet microscopy. Preliminary results from 3D morphology of the internal chlorophyll-a structure of two dinoflagellates species show promising application potentials of the method for phytoplankton taxonomy of selected species and species groups.. ©2013 Optical Society of America. Full Article , PDF Article ...
The objective of the proposed effort is to develop a novel technology that will provide a unique and powerful alternative to conventional flow cytometry. This new system, which we call a cytometer on a chip will spatially sort cells on a microarray chip based on the presence of specific cell surface receptors or other surface features. Detection and quantitation is accomplished with grating-coupled surface plasmon resonance imaging (GCSPR). This is a label-free detection technology that obviates the need for fluorescent or other types of labels. This feature and the fact that the technology is amenable to miniaturization and consumes very little power, makes the cytometer on a chip system ideally suited to the assessment of immune function in space and to cost-effective, rapid, on site clinical diagnostics. In the Phase I we will evaluate the feasibility of the proposed concept with a lymphocyte model system and we will ...
Doan, Minh and Szalóki, Nikoletta and Tóth, Katalin and Szöllősi, János and Bacsó, Zsolt and Vámosi, György (2014) FRET Imaging by Laser Scanning Cytometry on Large Populations of Adherent Cells. CURRENT PROTOCOLS IN CYTOMETRY, 70. 2.23.1-2.23.29. ISSN 1934-9297 Szalóki, Nikoletta and Doan, Minh and Szöllősi, János and Tóth, Katalin and Vámosi, György and Bacsó, Zsolt (2013) High throughput FRET analysis of protein-protein interactions by slide-based imaging laser scanning cytometry. CYTOMETRY PART A, 83 (9). pp. 818-829. ISSN 1552-4922 Klekner, Álmos and Scholtz, Beáta and Hanzély, Zoltán and Tóth, Judit and Kenyeres, Annamária and Ruszthi, Péter and Bognár, László and Szöllősi, János and Vereb, György and Varga, Imre and Hutóczki, Gábor and Petrás, Miklós (2009) Különböző eredetű malignus agydaganatok invazivitásának panelszerű vizsgálata. ...
To be able to effectively plan a C-section the due date should be carefully predicted BEFORE breeding. Accurate prediction of whelping date can be done by measuring the Serial blood progesterone or blood lutenizing hormone (LH). This means that the breeding should be coordinated with hormone and cytologic evaluation. Whelping usually occurs 65 days after the LH peak, with a variability of 1 day. Planning a C-section at the first signs of labor often equals to an emergency C-section, especially as bulldogs often produce early. For that reason breeders often book their bitches on the sixty-first day. However, opponents of elective C-section approach say this can cause problems, as most bitches are mated more than once during their seasons and the breeder has to be sure of exactly which mating the bitch conceived ...
BioAssay record AID 404667 submitted by ChEMBL: Cell cycle arrest in BAEC assessed as accumulation at G0/G1 phase at 200 uM after 24 hrs by flow cytometer relative to control.
The FACS Canto Flow Cytometer has two lasers an air cooled 20mW solid state blue laser which excites at 488nm. The other is a 17mW HeNe red laser which excites at 633nm which allows for 6-colour analysis. Current
Beckman Coulter has significantly expanded the workflow capabilities of its AQUIOS CL Flow Cytometer with the addition of the AQUIOS Designer Software.
Beckman Coulter has significantly expanded the workflow capabilities of its AQUIOS CL Flow Cytometer with the addition of the AQUIOS Designer Software.
mHealth in Resource-Constrained Environments: 10.4018/978-1-4666-8756-1.ch031: The use of mobile devices in telemedicine contributes to providing more effective and efficient remote healthcare in rural areas improving patients life
We have developed an inexpensive portable microarray reader that can be applied to standard microscope slide-based arrays and other array formats printed on chemically modified surfaces. Measuring only 19 cm in length, the imaging device is portable and may be applicable to both triage and clinical settings. For multiplexing and adaptability purposes, it can be modified to work with multiple excitation/emission wavelengths. Our device is shown to be comparable to a commercial laser scanner when detecting both streptavidin-biotin and antibody interactions. This paper presents the development and characterization of a handheld microarray imager and directly compares it with a commercial scanner.
A new study reveals that using multispectral photoacoustic imaging, a combination of laser optics and ultrasound imaging technologies, can help distinguish benign and malignant prostate tissue.
We present an analysis of flickering (2-10 Hz) auroras observed with a state-of-the-art multispectral imaging system, Auroral Structure and Kinetics, located in Tromso, Norway. Short (1-2 s) periods of flickering aurora have been identified in which the frequency of the brightness oscillations decreases or increases smoothly over time. To the authors knowledge this is the first detailed analysis of such "chirps" in flickering aurora or field-aligned bursts. We have found that the electron precipitation energy is strongly anticorrelated with the flickering frequency during all identified chirps. This result is consistent with the theory that flickering aurora is caused by the resonance interaction between electromagnetic ion cyclotron waves and precipitating electrons and that the wave parallel phase velocity is the primary factor determining the electron acceleration produced by this mechanism. Other current theories known to the authors cannot completely explain our ...
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HCS systems are basically fully automated microscopes: an automated stage moves the multi-well plate from position to position (according to a previously defined protocol) and at each position the same imaging procedure is performed. Dichroic mirrors and filters are automatically switched, the sample is illuminated at one or more excitation wavelengths and the images are recorded with a CCD camera. Optionally also brightfield images are recorded. A combination of a laser-based autofocus system and a software-based autofocus procedure ensure that the sample is always in focus during imaging. HCS systems are available in widefield configuration or as confocal setup as for certain experiments optical sections or 3D imaging is required. The confocal HCS systems usually employ a spinning disk technique to ensure fast image acquisition. For fluorescence excitation the systems are equipped either with Mercury or Xenon light sources or with lasers, which are ...
TechTalks.tv is making it super-easy to publish, search and learn from slide-based videos, all in order to share educational content on the web.
The MACSQuant Calibration Beads are designed for routine calibration of the MACSQuant® Flow Cytometers. These calibration particles are 2 and 3 μm in size and enable the adjustment of the voltage settings. In addition, the 3 μm size particles are stained with multiple fluorescent dyes, whose emission spectra are compatible with many commonly used fluorochromes for flow cytometry. The MACSQuant Calibration Beads are excited by light between 400 nm and 650 nm to yield emission in all fluorescent channels. This enables the successful calibration of the laser settings. - USA
To date, all of Art lenses feature Sigmas HSM (Hypersonic Motor) driving AF and this statement covers the 85 f/1.4 Art Lens. This lens predecessor also had an HSM AF system, but according to Sigmas press release, "A re-engineered AF system brings 1.3X the torque of its predecessor, while other features such as a full-time manual focus override have been added, which can be controlled when the focus ring is rotated, even during continuous AF." Without doing a side-by-side comparison, I cant tell if that "torque" difference is noticeable, but autofocus happens reasonably quickly with short distance changes. Focus at 3 (1m) and then at 20 (m) and you will not likely consider the word "fast" for a descriptor as you wait for the subject to come into focus. Especially in lower light levels, some minor autofocus distance adjustments are often made after the initial focus acquisition, increasing the overall AF lock times of even shorter distance adjustments. While this lens may not be the ...
The Nikon D200 is a 10.2-megapixel digital single-lens reflex camera that falls between entry-level/midrange DSLR cameras such as the Nikon D40, Nikon D40x, and D80 and high-end models such as the Nikon D2Hs and D2Xs. It was released by the Nikon Corporation in November 2005. The D200 was succeeded by the D300 in August 2007. Main advantages over the D40, D40x, D50, D60 and D80 cameras include: More robust, metal (magnesium alloy) body incorporating environmental seals Better matrix metering than D40, D50, or D80 (more sensor areas and less prone to overexposure) Higher image resolution than D50 and D40, 10 MP instead of 6 MP (Note that the D40x and D60 are 10.2 MP) 11-point autofocus system instead of a 3-point system (D40, D40x and D60) Advanced continuous autofocus tracking options More straightforward controls; many controls to adjust shooting settings (image quality, ISO, etc.) are directly accessible on the camera without going into the menu system Five frames per ...
Vironova is a Swedish biotechnology company providing comprehensive hardware, software, and services for the analysis of nanoparticles. Vironova revolutionizes access to transmission electron microscopy-based image analysis in biopharmaceutical development. Our solution enables automated analyses for faster and better-informed decisions to secure robust bioprocessing and final product quality.
Now that automated image-acquisition instruments (high-throughput microscopes) are commercially available and becoming more widespread, hundreds of thousands of cellular images are routinely generated in a matter of days. Each cellular image generated in a high-throughput screening experiment contains a tremendous amount of information; in fact, the name high-content screening (HCS) refers to the high information content inherently present in cell images (J Biomol Screen 2:249-259, 1997). Historically, most of this information is ignored and the visual information present in images for a particular sample is often reduced to a single numerical output per well, usually by calculating the mean per-cell measurement for a particular feature. Here, we provide a detailed protocol for the use of open-source cell image ...
An oncolytic virus, due to its ability to replicate and lyse cancer cells while leaving normal cells intact, is an example of targeted cancer therapy. Reovirus is intrinsically oncolytic without the need for any genetic manipulation due to its ability to target cells with an activated ras pathway. micro RNAs (miRNA) play critical role in both viral infection and oncogene activation. While significant advances in the role of miRNA in a variety of diseases including cancer have been made, the majority of the studies done on miRNA signatures have been done using homogenized tissue, typically via RT PCR. While useful, one drawback of this methodology is that it is, in essence, a population study, sampled from a heterogeneous collection of cells and tissue types. The miRNA signatures found via these methods are not able to determine whether the miR and target protein were found in the same tissue type (tumor, stroma, etc). Microscopy-based ...
Citation: Witt KL, Livanos E, Kissling GE, Torous DK, Caspary W, Tice RR, Recio L. Comparison of flow cytometry- and microscopy-based methods for measuring micronucleated reticulocyte frequencies in rodents treated with nongenotoxic and genotoxic chemicals. Mutat Res. 2008 Jan 8;649(1-2):101-13. Epub 2007 Aug 11 ...
LONDON - Today, various academic institutions, hospitals, government institutes and also pharma and biotech firms are increasing their usage of cell analysis as a modern emerging tool in research, diagnosis and drug discovery.. At present, PCR amid different molecular approaches captures the biggest share in the overall market, whilst sequencing is predicted to increase at the highest CAGR through 2023. Meantime, amid image-based approaches cytometry had the largest revenues, whilst FISH is predicted to increase at a double-digit CAGR through 2023. On the basis of applications, cellular process application held the biggest revenue share, however in the years ahead the single cell analysis market is anticipated to boom at a high CAGR through 2023.. At present, North America takes lead in the worlds market for cell analysis techniques. It is ...
Fluorescence images of Cy3-labeled random-sequence linear ODN probe showing that the ODN probes are not inside the mitochondrial inner membrane. After delivery
Fluorescence images show that Rhodamine-PNP were internalized in MiaPaCa-2 pancreatic cancer cells following 1 hour and 24 hours of treatment of the cells. Cell
Written by Tim Bushnell, Ph.D. Flow cytometry is a powerful technique impacting both clinical and research.. If youre looking for a career, flow cytometry technology can take you many places. An experienced flow cytometrist can find a job in a biotechnology company, in academia, or in a clinical setting.. Rather than focusing on specific career information, I would like to highlight one career option that has been very good for me since my transition from research scientist to core manager over a decade ago.. Core facilities, or Shared Resource Laboratories (SRL) as this Cytometry A paper made popular, represent an investment by institutions in resources and personnel.. Staff and directors working in SRLs have the advanced training and experience to support the researchers and research mission of the institution.. Working in an SRL is a different and very ...
Dr Filby obtained his PhD from the National Institute for Medical Research (NIMR) in Mill Hill, London where he studied the role of the Src family kinases Lck and Fyn in T cell function. He is currently head of the Flow Cytometry Core Facilit (FCCF) at Newcastle University leading a dedicated team of flow cytometry specialists with the sole aim of providing a comprehensive, cutting edge cytometry resource to the wider research community at Newcastle University and beyond. A significant part of his focus is the development of novel cytometry-based techniques that have underpinned several high profile publications in journals including Science and Cell. He also received the Cytometry Part A paper of the year accolade in 2011. He specialises in Imaging Flow ...
Flow Cytometry Clinical Applications Scientist. Locations: San Diego, CA, San Jose, CA and Tempe, AZ. Summary:. BD is looking for a Clinical Applications Scientist to provide advanced support for BD Biosciences (BDB) flow cytometry products. The ideal candidate will have expertise in a broad-range of Clinical Flow Cytometry applications, including leukemia and lymphoma, immunodeficiency, cross-match, stem cell enumeration, DNA ploidy, transplant medicine, and antibody-mediated therapy assays.. The Clinical Applications Scientist serves as a subject matter expert for strategic product applications in Flow Cytometry; provides pre- and post- sales support to sales associates based upon customer and BDB business needs. He/she takes part in system validation activities throughout ...
Flow cytometry is an invaluable tool used to analyze the chemical and physical properties of cells. This laboratory technique uses an antibody conjugated with a fluorochrome for cell analysis. The development of a specialized flow cytometer in the 1970s, triggered by the HIV pandemic, paved the way to a broad range of applications such as protein modifications studies, immunophenotyping, and analysis of intracellular antigens. The most important clinical application of flow cytometry is in hematologic malignancy diagnosis.
As a leading supplier of research products and custom services to global academic, pharmaceutical and biotech communities, Creative Diagnostics recently launches flow cytometry for all bio-researchers focusing on scientific study. The flow cytometry data is extremely quantitative and it provides fast, objective and simultaneous multi-parameter analysis of single cells as well as physical separation of cells of particular interest. This flow cytometry is for research use only, not for use in diagnostic procedures.. Flow cytometry is a laser-based technique to count and analyze the size, shape and properties of individual cells within a heterogeneous population of cells. Flow ...
Scintillon Institute. John Nolan is a Professor at the Scintillon Institute, where his group develops new cytometry instrumentation and applications. He was previously Director of the National Flow Cytometry Resource and is on the editorial boards of Cytometry and Current Protocols in Cytometry. He regularly teaches on cytometry topics via short courses and webinars and helps lead CYTO University, the online education effort of the International Society for Advancement of Cytometry (ISAC).. ...
Nous avons trouvé que le contenu en ADN nucléaire varie peu chez les lamproies. Dans les 3 familles principales ce contenu représente en moyenne le 40% de celui de lhomme. Il existe une corrélation entre le contenu en ADN et le caryotype dans presque tous les genres.. ...
To find the rotation between to images you need to know orientation of both, and therefore, the pose. To calculate the camera pose you need to find homography transformation from keypoints matches. Imagine you know the orientation of the first frame and position, because you decide it arbitrarily. You have the keypoints extracted by SIFT. From here you have next steps:. 1- Extract keypoints from next frame.. 2- Find matches of the keypoints on both frames.. 3- Use RANSAC to find the best set of inliers/outliers of that matches for next step. 4- Use DLT (Direct Lienar Transform) with that set, this will use 4 matches to find homography between images.. 5- Once you have homography, you can extract the pose, and the rotation.. You have openCV functions for all the steps except for pose from homography.. ...
13 MP Dual, f/2.0, laser & phase detection autofocus, Dual-LED flash, Geo-tagging, touch focus, face detection, HDR, panorama, Video, 2ndry 8 MP, f/2.2, 1.4 µm pixel size, ...

Babraham Institute » EquipmentBabraham Institute » Equipment

This imaging cytometer produces multiple high resolution images of every cell directly in flow, giving quantitative information ... spot counting and cell death analysis.. Members of the core facility are available to help set up and optimise assays and help ... All of the sorters in the BI Flow Cytometry Core Facility are available as part of the cell sorting service run by the facility ... 1 laser, 3 Parameters (532nm laser) This mini cytometer allows assessment of cell viability, apoptosis and cell cycle. The ...
more infohttps://www.babraham.ac.uk/science-services/flow-cytometry/equipment

JCI -
Smooth muscle cell-specific fibronectin-EDA mediates phenotypic switching and neointimal hyperplasiaJCI - Smooth muscle cell-specific fibronectin-EDA mediates phenotypic switching and neointimal hyperplasia

... and cell cycle was analyzed using flow cytometry. The panels show quantitative data of cell cycle distribution in quiescent and ... migration assay, 500 μm. (B) Representative Western blots and densitometric analysis of Akt1, mTOR, NF-κB, and β-actin (n = 5 ... Cells were then stimulated with PDGF or cFn (20 μg/mL) or pFn (20 μg/mL), and the second set of images was obtained after 12 or ... cFn-EDA-stimulated cells, §P , 0.05 vs. pFn + RGDS peptide-treated cells. Scale bars: BrdU assay, 50 μm; migration assay, 500 ...
more infohttps://www.jci.org/articles/view/124708

RMS |  Bio-imaging & Flow Cytometry Facility - University of LeedsRMS | Bio-imaging & Flow Cytometry Facility - University of Leeds

Common applications include cell cycle, ploidy analysis, proliferation, apoptosis, immunophenotyping, live/dead assays and ... Bio-imaging & Flow Cytometry Facility - University of Leeds. Bio-imaging & Flow Cytometry Facility - University of Leeds. ... Bio-imaging & Flow Cytometry Facility - University of Leeds Bio-imaging & Flow Cytometry Facility, Faculty of Biological ... The bio-imaging and flow cytometry facility is used by molecular, cell, plant and medical biologists, but we have a growing ...
more infohttps://www.rms.org.uk/network-collaborate/facilities-database/facilities-database-submission-form.html?slug=bio-imaging-flow-cytometry-facility-university-of-leeds

The novel KLF4/PLAC8 signaling pathway regulates lung cancer growth | Cell Death & DiseaseThe novel KLF4/PLAC8 signaling pathway regulates lung cancer growth | Cell Death & Disease

Luciferase reporter analysis and chromatin immunoprecipitation assays determined that KLF4 negatively regulated PLAC8 promoter ... In vitro and in vivo assays further revealed that endogenous PLAC8 promoted cell proliferation and tumor formation. We also ... found downregulated PLAC8 protein in several LC cell lines following the induction of KLF4, and immunohistochemistry analysis ... Flow cytometry for cell-cycle and cell apoptosis analysis. Cells were collected at 48 h after transfection with an siRNA or ...
more infohttps://www.nature.com/articles/s41419-018-0580-3?error=cookies_not_supported&code=bb9d7cbb-a47c-46ac-824c-5a0a2f34f4ae

Plus itPlus it

Cell-cycle analysis by flow cytometry. Cell-cycle analysis was conducted using propidium iodide (PI) following the ... D, MTT assay showing the effect of XPO1 inhibition (KPT-251) on cell proliferation after p53 knockdown in A375 melanoma cell ... Photomicrography images were recorded using a Nikon Eclipse 80i microscope and QImaging Retiga Exi chamber. Images were ... These finding correlate to our cell-cycle analysis suggesting a role for pRb in CRM1-mediated cell-cycle arrest. ...
more infohttps://mct.aacrjournals.org/content/12/7/1171

ProbesOnline, Cell Proliferation, Cell Cycle Imaging, Reporter Gene Assays | Thermo Fisher Scientific - USProbesOnline, Cell Proliferation, Cell Cycle Imaging, Reporter Gene Assays | Thermo Fisher Scientific - US

In this issue you will find new product information for Cell proliferation, cell cycle imaging, reporter gene assays ... Vybrant® DyeCycle™ Ruby Stain is used for cell cycle analysis in live cells by flow cytometry using the common 635 nm red laser ... FUCCI cell cycle sensor enables live-cell imaging of cell cycle and division. As cells progress through the cell cycle, nuclear ... Live-cell imaging of cell cycle and division. U2OS cells were transduced with Premo™ FUCCI Cell Cycle Sensor, then imaged the ...
more infohttps://www.thermofisher.com/us/en/home/references/newsletters-and-journals/probesonline/probesonline-issues-2009/probesonline-august-2009.html

Targeting Integrin-Linked Kinase Suppresses Invasion and Metastasis through Downregulation of Epithelial-to-Mesenchymal...Targeting Integrin-Linked Kinase Suppresses Invasion and Metastasis through Downregulation of Epithelial-to-Mesenchymal...

The cell cycle was analyzed in Caki-1 cells treated with si-ILK or control siRNA using FACS flow cytometry with PI staining. E ... we assayed EMT markers in ILK-overexpressing UMRC-6 cells (Fig 2A). Western blot analysis confirmed that ILK overexpression was ... B, representative images of stress fiber formation in UMRC-3 cells transfected with siScr or siILK. C, representative images of ... 3C). Cell-cycle analysis showed that ILK knockdown did not induce apoptosis or significant cell-cycle arrest in Caki-1 cells. ...
more infohttps://mct.aacrjournals.org/content/14/4/1024

Cell Division, Mitosis and Cell Cycle - Tools to help simplify cell division, mitosis and cell cycle research | Thermo Fisher...Cell Division, Mitosis and Cell Cycle - Tools to help simplify cell division, mitosis and cell cycle research | Thermo Fisher...

... mitosis and cell cycle research. Cell division analysis and control represents one of the most dynamic segments of biomedical ... Regulation of major events of the cell cycle-DNA synthesis, mitosis, and cell division-involves steps which are crucial to the ... cell, including detecting and repairing genetic damage and providing various checks to prevent uncontrolled cell division. ... Our cutting-edge tools can help simplify your cell division, ... Apoptosis Assays for Flow Cytometry › * Apoptosis for Imaging › ...
more infohttps://www.thermofisher.com/jp/en/home/life-science/cell-analysis/cell-viability-and-regulation/cell-cycle.html

Cell Cycle Analysis Market Report - Forecast to 2021 | MRFRCell Cycle Analysis Market Report - Forecast to 2021 | MRFR

It helps in distinguishing cells that are in different phases of cell cycle and used to determine the cellular response to ... Cell cycle analysis is commonly used in biomedical research studies and clinical diagnosis. ... Cytometry. *Flow Cytometry. *Instruments. *Consumables. *Assay. *Reagents & Kits. *Other Consumables. *Software. *Image ... The cell cycle analysisisperformedthroughmethods such as cytometry, cell imaging, cell counting, Microscopy, and others. ...
more infohttps://www.marketresearchfuture.com/reports/cell-cycle-analysis-market-673

MicroRNA-214-3p inhibits proliferation and cell cycle progression by targeting MELK in hepatocellular carcinoma and correlates...MicroRNA-214-3p inhibits proliferation and cell cycle progression by targeting MELK in hepatocellular carcinoma and correlates...

Flow cytometry analysis was conducted to investigate whether miR-214-3p could induce apoptosis of HCC cells. Increasing ... Cell proliferation assay. Cell proliferation assay was carried out using cell Titer 96 Aqueous one Solution Cell Proliferation ... Open image in new window. Fig. 3 MiR-214-3p overexpression inhibits cell proliferation and cell cycle progression in HUH-7 and ... Cell cycle analysis. HepG2 and HUH-7 cells were collected in the log phase of growth and incubated for 24 h. Then the cells ...
more infohttps://link.springer.com/article/10.1186/s12935-017-0471-1

Long non-coding RNA CASC15 promotes melanoma progression by epigenetically regulating PDCD4 | SpringerLinkLong non-coding RNA CASC15 promotes melanoma progression by epigenetically regulating PDCD4 | SpringerLink

5c). Flow cytometry analysis showed a G0-G1 cell cycle arrest and an increased apoptotic rate in A375 and M21 cells following ... Open image in new window. Fig. 4 CASC15 epigenetically silences PDCD4 expression by binding to EZH2. a Western blot assay was ... e, f Flow cytometry analysis was applied to detect cell cycle distribution in transfected A375 and M21 cells. The bar chart ... 2c, d). Cell cycle analysis uncovered that knockdown of CASC15 resulted in an evident cell cycle arrest at G0-G1 phase in A375 ...
more infohttps://link.springer.com/article/10.1186%2Fs13578-018-0240-4

Plus itPlus it

Flow cytometry. For cell-cycle analysis, cells were plated at densities between 1.5 and 3.5 million cells per dish in 10-cm ... In this study, we used image-based proliferation and apoptosis assays in colorectal cancer cell lines to systematically ... Colors indicate cell-cycle phase. E, Quantitative, real-time PCR analysis shows regulation of apoptosis and cell-cycle factors ... The combination modulated regulators of cell cycle and apoptosis. In quantitative real-time (qRT) PCR analyses of cell-cycle ...
more infohttp://cancerres.aacrjournals.org/content/76/23/6950

Delivery of RIPK4 small interfering RNA for bladder cancer therapy using natural halloysite nanotubes | Science AdvancesDelivery of RIPK4 small interfering RNA for bladder cancer therapy using natural halloysite nanotubes | Science Advances

Cell cycle assays. For cell cycle analysis, T24 cells were plated at a density of 5 × 104 cells per well in 24-well plates. At ... In T24 cells treated with various formulations, the cell cycle of was examined using flow cytometry. As shown in fig. S8, HNTs/ ... A) Bright-field and fluorescence microscopy images of HNTs/FAM-siRIPK4 fluorescence at 6 hours after transfection. (B) Flow ... Apoptosis analysis using flow cytometry. T24 cells were plated at a density of 3 × 105 per well in six-well plates. Forty-eight ...
more infohttps://advances.sciencemag.org/content/5/9/eaaw6499

Cell Cycle Analysis with the Celigo | Nexcelom BioscienceCell Cycle Analysis with the Celigo | Nexcelom Bioscience

Image and distinguish cell cycle phases using Propidium Iodide (PI), BrdU/DAPI and EdU/DAPI using the Celigo cytometer from ... cell imaging cytometer allows users to conduct cell cycle analysis on adherent cells using assays typically developed for flow ... cytometry. The flexible gating interface of the Celigo software is used to identify and quantify populations of cells in each ... Analyze cell cycle data using Celigo gating interface ~ 20 minutes. Results: Cell Cycle Assay using A375 Cells with ...
more infohttps://www.nexcelom.com/applications/celigo/fluorescent-assays/cell-cycle/

Leukemic blasts program bone marrow adipocytes to generate a protumoral microenvironment | Blood JournalLeukemic blasts program bone marrow adipocytes to generate a protumoral microenvironment | Blood Journal

The proliferation percentage of cycling AML blasts was analyzed using flow cytometry. Cell-cycle analysis of AMLs from ... Proliferation, cell cycle, and death assays. Treated primary AML blasts were incubated with 5-bromo-2′-deoxyuridine (BrdU ... Cells were imaged by an AxioCam ICm1 monochrome charge-coupled device camera attached to the Apotome.2 Imaging System and ... F) Nonmalignant CD34+ cells cultured alone or in coculture with BMSC or adipocytes and CD34+ cell counted using flow cytometry ...
more infohttp://www.bloodjournal.org/content/129/10/1320?ijkey=f9a90e6dde7a28921df5e0783e9ef705c31083f1&keytype2=tf_ipsecsha&sso-checked=true

Insights into antitrypanosomal drug mode-of-action from cytology-based profiling | classicistranieri.comInsights into antitrypanosomal drug mode-of-action from cytology-based profiling | classicistranieri.com

The assays included DNA-staining followed by microscopy and quantitative image analysis, or flow cytometry; terminal dUTP nick ... Thus, current antitrypanosomal drugs perturb distinct and specific cellular compartments, structures or cell cycle phases. ... brucei cellular assays to probe the MoA of the current HAT drugs. ... Correction: Epstein-Barr virus nuclear antigen EBNA-LP is essential for transforming naïve B cells, and facilitates recruitment ...
more infohttps://www.classicistranieri.com/insights-into-antitrypanosomal-drug-mode-of-action-from-cytology-based-profiling.html

YTHDF1 links hypoxia adaptation and non-small cell lung cancer progression.  - PubMed - NCBIYTHDF1 links hypoxia adaptation and non-small cell lung cancer progression. - PubMed - NCBI

j Effect of YTHDF1 knockdown on the G0/G1 cell population in A549 cells, as measured by PI staining and flow cytometry. ... j, k Representative images of Ki67 and CC3 immunostaining (j) of lung tumors and statistical analyses of the Ki67- and CC3- ... such as cell cycle regulators including CDK2, CDK4, cyclin D1, etc., in cancerous cells, which causes uncontrolled cancerous ... l Quantification data for the EdU assays in A549 and H1299 cell lines. m Effect of YTHDF1 knockdown on protein levels of the G0 ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/31653849

Breast tumor profile | definition of breast tumor profile by Medical dictionaryBreast tumor profile | definition of breast tumor profile by Medical dictionary

... tests in a BTP include estrogen and progesterone receptor assays, DNA index/cell cycle analysis by flow cytometry or by image ... analysis and Ki-67 localization, cathepsin D, c-erbB-2/HER-2/neu oncoprotein analysis. Cf Breast cancer profile. ...
more infohttp://medical-dictionary.thefreedictionary.com/breast+tumor+profile

Homocysteine Activates B Cells via Regulating PKM2-Dependent Metabolic Reprogramming | The Journal of ImmunologyHomocysteine Activates B Cells via Regulating PKM2-Dependent Metabolic Reprogramming | The Journal of Immunology

... as evidenced by fluorescent images and quantification of fluorescence intensity from flow cytometry analysis (Fig. 2G, 2H). ... Plasma Hcy levels were quantified via an enzymatic cycling assay as previously described (26). Total plasma triglycerides and ... F) Purified B cells were labeled with CFSE prior to culture and cell proliferation was assessed by flow cytometry after 48 h. ... Proliferation of CFSE-labeled B cells (A) and CD138+ plasma cells (B) were identified via flow cytometry at 48 h. IgM (C) and ...
more infohttps://www.jimmunol.org/content/198/1/170

Hematopoietic stem and progenitor cells regulate the regeneration of their niche by secreting Angiopoietin-1 | eLifeHematopoietic stem and progenitor cells regulate the regeneration of their niche by secreting Angiopoietin-1 | eLife

Normal hematopoietic stem and progenitor cells slow the regeneration of their niche after injury to minimize leakage from the ... were used to stain cells for analysis by flow cytometry.. Cell cycle analysis. For BrdU incorporation assays, mice were given ... Stem Cells and Regenerative Medicine Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of ... By flow cytometry, only 1.5 ± 0.8% of mechanically dissociated bone marrow cells (which include few stromal cells) were GFP+ ( ...
more infohttps://elifesciences.org/articles/05521

Frontiers | Activation of Epithelial-Mesenchymal Transition and Altered β-Catenin Signaling in a Novel Indian Colorectal...Frontiers | Activation of Epithelial-Mesenchymal Transition and Altered β-Catenin Signaling in a Novel Indian Colorectal...

In-depth molecular analysis of MBC02 revealed suppression of E-cadherin expression, concomitant with overexpression of EMT ... We have established a novel cell line, MBC02, from an Indian colorectal cancer patient and have carried out extensive molecular ... In-depth molecular analysis of MBC02 revealed suppression of E-cadherin expression, concomitant with overexpression of EMT ... related molecules, which manifested in the form of highly migratory and invasive cells. Loss of membrane-tethered E-cadherin ...
more infohttps://www.frontiersin.org/articles/10.3389/fonc.2019.00054/full

TFF3 Contributes to Epithelial-Mesenchymal Transition (EMT) in Papillary Thyroid Carcinoma Cells via the MAPK/ERK Signaling...TFF3 Contributes to Epithelial-Mesenchymal Transition (EMT) in Papillary Thyroid Carcinoma Cells via the MAPK/ERK Signaling...

Cell cycle analysis. For flow cytometry, the TFF3KD#1, TFF3KD#2 and shCon TPC-1 cells were fixed with 70% ethanol for 12 h at 4 ... cell adhesion assays, colony formation assays, wound-healing assays and transwell chamber invasion assays. EMT markers and ... Click on the image to enlarge.). Figure 3 Silencing of TFF3 inhibits TPC-1 cell proliferation and arrests G1 phase. TFF3 ... Cell cycle was assessed by a flow cytometer. The distribution of cell cycle in TFF3KD#1 and TFF3KD#2 changed and the proportion ...
more infohttp://jcancer.org/v09p4430.htm

Campylobacter jejuni promotes colorectal tumorigenesis through the action of cytolethal distending toxin | GutCampylobacter jejuni promotes colorectal tumorigenesis through the action of cytolethal distending toxin | Gut

... comet assay, immunofluorescence detection of γH2AX in vitro, γH2AX flow cytometry and cell cycle analysis are in the online ... A) Representative images of γH2AX immunofluorescence staining, (B) γH2AX flow cytometry histograms, (C) comet assay and (D) ... cell cycle analysis by flow cytometry revealed that while C. jejuni-WT promotes G2/M cell cycle arrest, cells exposed to ... Cells were incubated with lysates (5 µg/mL) or PBS for 24 hours for γH2AX staining and comet assay, or 48 hours for cell cycle ...
more infohttps://gut.bmj.com/content/68/2/289

Frontiers | Berberine Attenuated Proliferation, Invasion and Migration by Targeting the AMPK/HNF4α/WNT5A Pathway in Gastric...Frontiers | Berberine Attenuated Proliferation, Invasion and Migration by Targeting the AMPK/HNF4α/WNT5A Pathway in Gastric...

We found that in AGS and SGC7901 cell, BBR inhibited cell proliferation through downregulating C-myc. BBR also induced G0-G1 ... We found that in AGS and SGC7901 cell, BBR inhibited cell proliferation through downregulating C-myc. BBR also induced G0-G1 ... induced S phase arrest and dramatically attenuated gastric cancer cells metastasis and invasion. (3) We performed GC cells ... induced S phase arrest and dramatically attenuated gastric cancer cells metastasis and invasion. (3) We performed GC cells ...
more infohttps://www.frontiersin.org/articles/10.3389/fphar.2018.01150/full

Hibiscus syriacus Extract from an Established Cell Culture Stimulates Skin Wound HealingHibiscus syriacus Extract from an Established Cell Culture Stimulates Skin Wound Healing

Cell Cycle Analysis. Flow cytometry analysis was performed as previously described [20]. Briefly, cells seeded at a density of ... MTT Assay. HDF or HaCaT cells, plated into 96-well plates at a density of 1.3 × 103 cells/well, were grown for 12 h and treated ... d) For each condition, the unclosed cell-free areas at time 0 were compared to those at 7 h by using the software Image J. The ... Figure S2: Analysis of cell viability in HaCaT cells after treatment with HSEE. Figure S3: Cell cycle profiling of HaCaT cells ...
more infohttps://www.hindawi.com/journals/bmri/2017/7932019/
  • As well as having 5 lasers for 18 colour analysis this instrument was configured to maximize the use of BD Brilliant UV dyes. (babraham.ac.uk)
  • This machine is proving an invaluable asset as it allows scientists to maximise data acquired from each sample, saving time, money and valuable cells/tissues. (babraham.ac.uk)
  • Further investigation revealed that the Akt-mechanistic target of rapamycin signaling pathway was involved in this process, as the mechanistic target of rapamycin inhibitor rapamycin inhibited Hcy-induced changes in PKM2 enzyme activity and B cell activation. (jimmunol.org)
  • Lin X, Zhang H, Dai J, Zhang W, Zhang J, Xue G, Wu J. TFF3 Contributes to Epithelial-Mesenchymal Transition (EMT) in Papillary Thyroid Carcinoma Cells via the MAPK/ERK Signaling Pathway. (jcancer.org)
  • However, no prior reports have described either the role of CLDN2 on the malignancy phenotype of OS cells or whether the impact of CLDN2 is related to the afadin/ERK signaling pathway. (springermedizin.de)
  • The PI3K signaling pathway is frequently deregulated in cancer cells, and its constitutive activity strongly contributes to the oncogenic process ( 3 ). (aacrjournals.org)
  • Enhanced cytotoxicity was observed when additional processes including apoptosis and cell cycle were targeted as part of the combination. (aacrjournals.org)
  • In some cases, where cell lines were resistant to paired and tripled drugs, increased expression of antiapoptotic proteins was observed, requiring a fourth-order combination to induce cytotoxicity. (aacrjournals.org)
  • Our data suggest that p53-deficient cells are more susceptible to EMD-1214063 and combined treatment with irradiation than wild-type p53 lines as inferred from elevated γH2AX expression and increased cytotoxicity. (aacrjournals.org)
  • Using mathematical models coupled with cytotoxicity assays, we first identified concentration ratios of gemcitabine and CHK1 inhibitors that induced synergistic growth inhibition. (aacrjournals.org)
  • We conclude that type I IFN can protect cancer cells from T cell-mediated cytotoxicity through regulation of Serpinb9 . (jci.org)
  • A data analysis template created specifically for this application allows for the determination of both genotoxicity and cytotoxicity following treatment with known clastogens and aneugens. (merckmillipore.com)
  • Therefore, we have analyzed the ability of two PPAR γ ligands,15dPGJ2 and rosiglitazone, a natural and a synthetic PPAR γ ligand, respectively, to increase the adriamycin-induced cytotoxicity of PC-3 cells and to suppress the IGF-1 survival effect on adriamycin-induced apoptosis of PC-3 cells. (springer.com)
  • Herein we have analyzed the ability of PPARγ ligands, namely rosiglitazone and 15-deoxy-Δ 12, 14-prostaglandin J2 (15dPGJ2), a synthetic and a natural ligand of PPARγ, respectively, to act synergistically on adriamycin-induced cytotoxicity of PC-3 cells and to neutralize the type 1 IGF receptor (IGF-1R)-mediated intracellular survival signaling on the adriamycin-induced apoptosis of PC-3 prostate cancer cells. (springer.com)
  • The central mode of cytotoxicity was mitochondria-mediated apoptosis via inhibition of H + -ATP synthase activity and mitochondrial membrane potential depolarization, which in turn was associated with reduced intracellular ATP levels, caspase activation, elevated sub-G 1 cell population, and chromatin condensation. (aspetjournals.org)
  • In this study, the potential cytotoxicity on MSC proliferation, apoptosis, cell cycle, and in vitro differentiation were assayed following 2.856 GHz microwave exposure at a specific absorption rate (SAR) of 4 W/kg. (plos.org)
  • The current findings reveal that Bakuchiol is a potent cytotoxic agent against gastric cancer cells and its cytotoxicity is mediated through induction of apoptosis and sub-G1 cell cycle arrest. (alliedacademies.org)
  • The epithelial-mesenchymal transition (EMT) has been regarded as a vital step in the process of carcinoma metastasis from the primary site to distant tissues or lymph nodes, by which epithelial cells gradually acquire mesenchymal features and enhance migratory and invasive properties[ 5 , 6 ]. (jcancer.org)
  • Skin dryness alters the ability of epithelial cells to migrate and cover the wound site and reduces the supply of white blood cells and nutrients, which are essentials to form new tissues and protect skin against infections [ 3 ]. (hindawi.com)
  • Endowment of the epithelial cells with mesenchymal characteristics allows them to migrate and invade secondary tissues in order to form malignant sites and also confers chemoresistance. (chemweb.com)
  • Fusobacterium nucleatum promotes tumor formation by epithelial cells via unclear mechanisms. (nih.gov)
  • Epithelial cells expressing either β1 or β3 integrins, in which p53 activity is suppressed, undergo G 2 arrest but show little apoptosis after treatment with cisplatin or other genotoxicants. (aspetjournals.org)
  • Furthermore, intestinal microbiota, which include various species of Lactobacillus , interact regularly with cells of the colon, which include professional antigen-presenting cells and intestinal epithelial cells ( 8 - 10 ). (pnas.org)
  • In colon cancer, PLAC8-overexpressing cells exhibited increased phosphorylated extracellular signal-regulated kinase 2, which led to elevated cell motility and cancer invasion 12 . (nature.com)
  • The cell cycle analysis market is expected to grow at high growth rate due to corporate & government support in cell-based researches, high prevalence of chronic genetic diseases, growing healthcare industry and increasing medical spending, rising incidence of cancer, and technologically advancements. (marketresearchfuture.com)
  • Our results illustrate how high-order drug combinations are needed to kill drug-resistant cancer cells, and they also show how systematic drug combination screening together with a molecular understanding of drug responses may help define optimal cocktails to overcome aggressive cancers. (aacrjournals.org)
  • Small interfering RNA (siRNA) has shown potential as a molecular approach to down-regulate specific gene expression in cancer cells ( 1 , 2 ). (sciencemag.org)
  • Furthermore, m 6 A modified mRNA binding protein YTHDF1, one of evolutionary positively selected genes for high-altitude adaptation is amplified in various cancers, including non-small cell lung cancer (NSCLC). (nih.gov)
  • 1) AGS and SGC7901 gastric cancer cells were treated with Berberine (BBR). (frontiersin.org)
  • The results demonstrated that the knockdown of HNF4α in SGC7901 slowed cells proliferation, induced S phase arrest and dramatically attenuated gastric cancer cells' metastasis and invasion. (frontiersin.org)
  • H. syriacus extract was previously shown to have antioxidant capacity [ 6 ] and antiproliferative effects on human lung cancer cells [ 7 ]. (hindawi.com)
  • Recent studies have revealed that many molecules are deeply involved in the oral squamous cell carcinoma formation and metastasis ( 4 - 7 ), which provide the genetic therapeutic targets for treatment of this kind of cancer. (medsci.org)
  • The expression level of NLK is aberrantly upregulated in hepatocellular carcinoma ( 14 ) while downregulated in the prostate cancer cells ( 15 ). (medsci.org)
  • The receptor is expressed in most human cancer cell lines and its growth can be inhibited by 1 α ,25(OH) 2 D 3 [ 10 - 14 ]. (hindawi.com)
  • However, commonly used PS induce death but also resistance pathways in cancer cells and an alteration of surrounding normal tissues. (chemweb.com)
  • Because polyamines (PA) are actively accumulated in cancer cells by the Polyamine Transport System (PTS), they may enable PS to specifically target cancer cells. (chemweb.com)
  • Here, we investigated whether new protoporphyrin IX-polyamine derivatives were effective PS against prostate cancer and whether PA increased PDT specificity after 630 nm irradiation.CHO and CHO-MG cells (differing in their PTS activity) were used to assess efficacy of polyamine vectorization. (chemweb.com)
  • Finally, PS effects were studied on tumor growth in nude mice.Our PS were more effective on cancer cells compared to non-malignant cells and more effective than PpIX alone. (chemweb.com)
  • Here, we studied the role of tumor endothelium on ovarian cancer cells (OCCs). (biomedcentral.com)
  • Activation of Notch3 dependent pathway in ovarian cancer regulates ovarian cancer cells (OCC) adhesion to peritoneal cells and cancer cell metastatic outgrowth [ 11 ]. (biomedcentral.com)
  • Ovarian cancer cell lines Skov3 were purchased from ATCC and cultured following ATCC recommendations (ATCC, Manassas, VA, USA). (biomedcentral.com)
  • A primary ovarian cancer cell line was derived in our laboratory from ascites of a patient with Stage III serous adenocarcinoma (APOCC). (biomedcentral.com)
  • However, it is unclear how immunological cues trigger cancer cell-intrinsic signaling pathways for entering into dormancy. (jci.org)
  • We explored the antitumor effects of AB, which was shown specifically to induce mitotic delay in pharyngeal (Detroit 562) and nasopharyngeal (Hone-1) cancer cells. (medsci.org)
  • Proliferation of cancer cells after exposure to aqueous extract of A. bracteosa (AEAB) was assessed using the MTT assay. (medsci.org)
  • In addition, AEAB induced the accumulation of G 2 /M-phase cells in cancer cell through the disassociation of CDC2/cyclin B1 complex. (medsci.org)
  • In conclusion, AEAB inhibits the growth of and induces mitotic delay in cancer cells, supporting its use as an anticancer agent. (medsci.org)
  • Poly (ADP-ribose) polymerase (PARP) plays key roles in the repair of DNA single-strand breaks, and a PARP inhibitor induces synthetic lethality in cancer cells with HR deficiency. (biomedcentral.com)
  • We examined the anti-tumor activity of olaparib, a PARP inhibitor, and its correlation between the sensitivity and status of PTEN in endometrial cancer cell lines. (biomedcentral.com)
  • Our results suggest that olaparib, a PARP inhibitor, is effective on certain endometrial cancer cell lines. (biomedcentral.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • While 2 Gy radiation has been shown to cause cancer cell death, studies also suggest a protective potential by low dose radiation. (oncotarget.com)
  • Here we aimed at studying the potential therapeutic application of ionizing radiation in combination with a MET inhibitor, EMD-1214063, in p53-deficient cancer cells that harbor impaired G 1 -S checkpoint regulation upon DNA damage. (aacrjournals.org)
  • Because cancer cells frequently harbor surveillance defects, they are reliant on the residual functional processes of the DDR machinery ( 2 ). (aacrjournals.org)
  • Chemotherapy often relies on cancer cell death resulting from DNA damage. (aspetjournals.org)
  • Most chemotherapeutic treatments rely on cancer cell death in response to DNA damage, although many genotoxic compounds also activate stress pathways independently of DNA damage, for example by inducing reactive oxygen species or binding to proteins within cells. (aspetjournals.org)
  • The final outcome of treatment with genotoxicants will also be determined to a large extent by other oncogenic pathways present in the tumor cells as well as by interactions with the cancer microenvironment. (aspetjournals.org)
  • Cancer) cells interact with the ECM through a family of receptors called integrins. (aspetjournals.org)
  • Here we show that resistance to an effective antitumor immune response is also a result of IFN signaling in a different cellular compartment of the tumor, the cancer cells themselves. (jci.org)
  • We abolished type I IFN signaling in cancer cells by genetic elimination of its receptor, IFNAR1. (jci.org)
  • Pronounced immune responses were provoked after ionizing radiation of tumors from 4 mouse cancer cell lines with Ifnar1 knockout. (jci.org)
  • Drug-like molecules that can reprogram selected histone PTMs in tumour cells are therefore of interest as potential cancer chemopreventive agents. (biomedsearch.com)
  • In this study we assessed the effects of the phytocompounds garcinol and curcumin on histone and p53 modification in cancer cells, focussing on the breast tumour cell line MCF7. (biomedsearch.com)
  • CONCLUSION: In summary, although garcinol and curcumin can both inhibit histone acetyltransferase activities, our results show that these compounds have differential effects on cancer cells in culture. (biomedsearch.com)
  • CD4 + and CD8 + T cells have distinct functions in the protection against pathogens, in addition to their importance in detecting cancer and regulating autoimmunity ( 2 - 4 ). (jimmunol.org)
  • Over 20 years academic research experience in cancer genetics, hemopoietic malignancies and stem cell biology. (visualcv.com)
  • The integrative approach of the ToxTracker assay provides a powerful tool for in vitro cancer hazard screening of chemicals by unveiling activation of specific cellular signalling pathways upon exposure and deliver insight into the underlying mechanism of toxicity. (merckmillipore.com)
  • Monitoring immune cell activity - including phenotyping immune cell subsets, tracking cell proliferation, and measuring cytokine production - can provide insights into the overall status of immune function in patients, particularly those undergoing immunosuppression after transplants, enduring cancer treatment, or suffering from autoimmune disease or other pathologies that affect the immune system. (merckmillipore.com)
  • Activation of programmed cell death in cancer cells offers novel and potentially useful approaches to improving patient responses to conventional chemotherapy. (aacrjournals.org)
  • In this study, we investigated the effect of XIAP down-regulation by antisense oligonucleotides (AS ODNs) on human non-small cell lung cancer (NIH-H460) growth in vitro and in vivo . (aacrjournals.org)
  • These studies support the contention that XIAP is a viable target for cancer therapy in human non-small cell lung cancer. (aacrjournals.org)
  • Expression of AGK in PC-3 prostate cancer cells markedly increased formation and secretion of LPA. (rupress.org)
  • We hypothesized that a super combination (SC) of known phytochemicals used at bioavailable levels could induce 100% killing of breast cancer (BC) cells without toxic effects on normal cells and that microarray analysis would identify potential genes for targeted therapy of BC. (jcancer.org)
  • One of the primary causes of this high rate of tumor recurrence and mortality is due to a small population of cancer stem cells (CSC) that evade therapy. (jcancer.org)
  • The aim of this study is to investigate the effects of polyphenol extract from Phyllanthus emblica (PEEP) on cervical cancer cells and to explore the underlying mechanism. (biomedcentral.com)
  • In the present study, we isolated polyphenol extract from PE (PEEP), and measured its effect on the proliferation, cell cycle and apoptosis of cervical cancer (HeLa) cells. (biomedcentral.com)
  • Insulin-like growth factor 1 (IGF-1) inhibits the adriamycin-induced apoptosis of PC-3 human prostate cancer cells. (springer.com)
  • A potential therapeutic agent for human head and neck cancer (HNC), cetrimonium bromide (CTAB), was identified through a cell-based phenotype-driven high-throughput screen (HTS) of 2000 biologically active or clinically used compounds, followed by in vitro and in vivo characterization of its antitumor efficacy. (aspetjournals.org)
  • Potential hit compounds were further evaluated for their anticancer specificity and efficacy in combination with standard therapeutics on a panel of normal and cancer cell lines. (aspetjournals.org)
  • FaDu (human hypopharyngeal squamous cell cancer), A549 (non-small-cell lung adenocarcinoma), MCF7 (breast adenocarcinoma), and MRC5 (normal lung fibroblasts) cell lines were obtained from the American Type Culture Collection (Manassas, VA). GM05757 (human primary normal) fibroblasts were obtained from the Coriell Institute for Medical Research (Camden, NJ). (aspetjournals.org)
  • Here, we aimed to identify the association between pseudogene derived lncRNA DUXAP8 and growth of pancreatic cancer cells. (biomedcentral.com)
  • We screened for pseudogene derived lncRNAs associated with human pancreatic cancer by comparative analysis of three independent datasets from GEO. (biomedcentral.com)
  • Loss-of-function approaches were used to investigate the potential functional roles of DUXAP8 in pancreatic cancer cell proliferation and apoptosis in vitro and in vivo. (biomedcentral.com)
  • RNA immunoprecipitation, chromosome immunoprecipitation assay and rescue experiments were performed to analyze the association of DUXAP8 with target proteins and genes in pancreatic cancer cells. (biomedcentral.com)
  • Our previous study showed that the expression of UBE2D3 was negatively related to human telomerase reverse transcriptase (hTERT) and radioresistance in human breast cancer cells. (jcancer.org)
  • Our previous study showed that the ubiquitin conjugating enzyme UBE2D3 was negatively related to radioresistance in breast cancer cells [ 8 ]. (jcancer.org)
  • Tocotrienol, an isomer of vitamin E, has demonstrated apoptotic and cell cycle arrest effects on cancer cells. (gsu.edu)
  • In addition, by suppressing the expression of Linc01194 in colon cancer cell lines, cell proliferation and migration were inhibited. (dovepress.com)
  • Linc01194 promotes the proliferation and migration ability of colon cancer cells by activating EMT. (dovepress.com)
  • 4 , 5 PVT1 is over expressed in colon cancer tissues compared to adjunct tissues and promotes cell proliferation and migration through the miR-30d-5p/runt-related transcription factor 2 (RUNX2) axis. (dovepress.com)
  • Combined with the above analysis, we hypothesized that Linc01194 functions as an oncogene in colon cancer. (dovepress.com)
  • Epidemiological and clinical trials rarely provide evidence for mechanisms of the compound's action, and studies on its effect on cancer of different cell origins are now being done. (biomedsearch.com)
  • RESULTS: Our data showed a significant decrease in the number of viable cells in three cancer cells lines (HT-29, T84 and MCF-7) after 48 h treatment with lycopene, and changes in the fraction of cells retained in different cell cycle phases. (biomedsearch.com)
  • These observations suggest that lycopene may alter cell cycle regulatory proteins depending on the type of cancer and the dose of lycopene administration. (biomedsearch.com)
  • Furthermore, we found PH domain and leucine-rich repeat protein phosphatase 2 (PHLPP2) to be a new target of miR-27a, and downregulation of PHLPP2 could rescue the effect of anti-miR-27a in gastric cancer cells. (biomedcentral.com)
  • For this reason, Fanconi anemia should be considered in patients presenting with aplastic anemia, squamous cell carcinomas presenting at an unusually young age, excessive side effects from chemotherapy or radiation, a personal or family history of cancer predisposition, a family history of cytopenias, or cytopenias together with congenital anomalies. (renalandurologynews.com)
  • MiRNAs directly or indirectly regulate the expression of many target mRNAs simultaneously, playing a critical role in the tumorigenesis, invasion, metastasis, and chemoresistance of cancer cells [ 11 - 13 ]. (ijbs.com)
  • Bakuchiol inhibits cell proliferation and induces apoptosis and cell cycle arrest in SGC-7901 human gastric cancer cells. (alliedacademies.org)
  • The objective of the current study was to investigate the anticancer and apoptotic effects of bakuchiol in SGC-7901 human gastric cancer cells along with evaluating its role in inducing cell cycle arrest in these cells. (alliedacademies.org)
  • EMT is characterized by a combined loss of epithelial cell junction proteins, such as E-cadherin, and the gain of mesenchymal markers, such as vimentin, and is believed to play an essential role in tumor invasion and metastasis. (aacrjournals.org)
  • In this study, we used molecular biological and other techniques to study the role and mechanisms of CLDN2 in OS cell metastasis and to identify novel targets for OS treatment and the control of early metastasis. (springermedizin.de)
  • Furthermore, the SC down-regulated the expression of PCNA, Rb, CDK4, BcL-2, SVV, and CD44 (metastasis inducing stem cell factor) in the BC cell lines. (jcancer.org)
  • It can promote proliferation and metastasis of tumor cells by suppressing PHLPP2 and activating the AKT/GSK3β pathway. (biomedcentral.com)
  • In addition, the underlying mechanisms by which MARCH5 regulated BC cell growth and metastasis were explored. (dovepress.com)
  • MARCH5 promoted the growth and metastasis of BC cells both in vitro and in vivo by inducing G1-S cell cycle arrest and epithelial-mesenchymal transition. (dovepress.com)
  • The Nemo-like kinase (NLK) is a serine/threonine-protein kinase that involved in a number of signaling pathways regulating cell fate. (medsci.org)
  • Evidence shows that NLK is involved in a number of important signaling pathways and regulates many transcription factors important for cell fate. (medsci.org)
  • The development of new therapies is thus highly desirable, and the targeting of signaling pathways that are preferentially activated in transformed AML cells represents a rapidly expanding research field in this regard ( 1 ). (aacrjournals.org)
  • To address these issues, we made use of a panel of cell lines derived from poorly adhesive, integrin β1 knockout cells that were immortalized using simian virus 40 Large T (inactivation of p53 and Rb pathways). (aspetjournals.org)
  • The blockage of β 2 -adrenoceptor markedly induced PanCa cells to arrest at G 1 /S phase and consequently resulted in cell death, which is possibly due to that the blockage of β 2 -adrenoceptor inhibited NFκB, extracellular signal-regulated kinase, and Akt pathways. (biomedcentral.com)
  • NNK functions as a β-adrenergic agonist and it has been shown that the binding of NNK or catecholamines to the β-adrenoceptors induce PanCa cell proliferation by activating the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) pathways in PanCa cells [ 15 ]. (biomedcentral.com)
  • These results emphasize a potentially important role for lactobacilli in modulating immunological functions of DCs and suggest that certain strains could be particularly advantageous as vaccine adjuvants, by promoting DCs to regulate T cell responses toward T helper 1 and Tc1 pathways. (pnas.org)
  • Imaging flow cytometry (IFC) has emerged as a useful and efficient tool for studying the signaling pathways in immunophenotypically defined subpopulations of immune cells. (merckmillipore.com)
  • Analysis of cell-nanosilicate interactions by monitoring changes in transcriptome profile uncovered key biophysical and biochemical cellular pathways triggered by nanosilicates. (pnas.org)
  • RNA-binding proteins (RBPs) have emerged as important regulators of invertebrate adult stem cells, but their activities remain poorly appreciated in mammals. (bloodjournal.org)
  • Western blot analysis was applied to detect the expression of proteins in the cells. (spandidos-publications.com)
  • To identify the subcellular forms and biochemical events induced in human cells after HCV polyprotein expression, we have used a robust cell culture system based on vaccinia virus (VACV) that efficiently expresses in infected cells the structural and nonstructural proteins of HCV from genotype 1b (VT7-HCV7.9). (biomedcentral.com)
  • Biochemical analysis demonstrate that HCV proteins bring about the activation of initiator and effector caspases followed by severe apoptosis and mitochondria dysfunction, hallmarks of HCV cell injury. (biomedcentral.com)
  • The ligands of EGFR consist of a family of structurally and functionally related integral membrane proteins that can be proteolytically cleaved and shed from cells ( 18 , 32 ). (physiology.org)
  • Aquaporins are a family of water channel proteins that provide a major pathway for osmotically driven water transport through cell membranes. (biologists.org)
  • Several potent endogenous proteins that inhibit apoptosis have been identified, including Bcl-2, and IAP families in mammalian cells. (aacrjournals.org)
  • We also assessed karyomorphism of the cells after incubation with PEEP for 48 hours, and assessed expression of three apoptotic marker proteins: Fas, FasL, and cleaved caspase-8, using western blotting. (biomedcentral.com)
  • Dynamic interaction with the immune system profoundly regulates tumor cell dormancy. (jci.org)
  • Additionally, in melanoma patients, high expression of IFN-β correlated with tumor cell dormancy. (jci.org)
  • On days 5, 10, and 20, tumor cell-injected tissues were analyzed by immunostaining against S100β or H&E staining. (jci.org)
  • G ) The same as E , except that at day 20, tissues with tumor cell inoculation were immunostained with anti-NR2F1, -Ki67, and DAPI ( n = 5). (jci.org)
  • Apoptosis is directly involved in tumor cell death after treatment with chemotherapeutic agents and radiation and is a process that requires the activation of proteolytic enzymes termed caspases. (aacrjournals.org)
  • The results suggest that this non-toxic bioflavonoid may be useful as a chemotherapeutic, particularly in combination with agents that target other components of the tumor cell cycle and in situations where estrogen receptor-specific therapeutics are ineffective. (biomedcentral.com)
  • detected UBE2D3 mRNA expression in 25 tumor cell lines and 24 normal cell lines and showed that UBE2D3 mRNA expression was significantly lower in tumor cell lines than in normal cell lines [ 12 ]. (jcancer.org)
  • The main purpose of the present study was to evaluate the antiproliferative activity of Bakuchiol in human gastric tumor cell lines (SGC-7901) along with demonstrating its mode of action. (alliedacademies.org)
  • It helps in distinguishing cells that are in different phases ofcell cycle and used to determine the cellular response to biological stimulations and various drug. (marketresearchfuture.com)
  • Here, we used a panel of T . brucei cellular assays to probe the MoA of the current HAT drugs. (classicistranieri.com)
  • Thus, current antitrypanosomal drugs perturb distinct and specific cellular compartments, structures or cell cycle phases. (classicistranieri.com)
  • The restoration of tissue integrity is the result of the interaction between several distinct cellular elements (keratinocytes, fibroblasts, monocytes/macrophages, and endothelial cells) and extracellular matrix (ECM) components, such as fibronectin and collagen whose contraction encourages the edges of the wound to shrink together [ 1 ]. (hindawi.com)
  • They look for these drugs by observing how compounds affect cellular mechanisms, using medium-throughput assays to perform multiparametric analysis of the cell cycle. (photonics.com)
  • Microarray analysis revealed that HCV polyprotein expression modulated transcription of genes associated with lipid metabolism, oxidative stress, apoptosis, and cellular proliferation. (biomedcentral.com)
  • The cellular DNA content of individual cells is often plotted as their frequency histogram to provide information about relative frequency (percentage) of cells in the major phases of the cell cycle. (wikipedia.org)
  • Multiparameter analysis of the cell cycle includes, in addition to measurement of cellular DNA content, other cell cycle related constituents/features. (wikipedia.org)
  • The concurrent measurement of cellular DNA and RNA content, or DNA susceptibility to denaturation at low pH using the metachromatic dye acridine orange, reveals the G1Q, G1A, and G1B cell cycle compartments and also makes it possible to discriminate between S, G2 and mitotic cells. (wikipedia.org)
  • Microarray analysis revealed several differentially expressed key genes (PCNA, Rb, CDK4, Bcl-2, SVV, P53 and CD44) underpinning SC-promoted BC cell death and motility. (jcancer.org)
  • Regulation of major events of the cell cycle-DNA synthesis, mitosis, and cell division-involves steps which are crucial to the cell, including detecting and repairing genetic damage and providing various checks to prevent uncontrolled cell division. (thermofisher.com)
  • Here we intended to elucidate the functional implication of miR-214-3p in regulation of cell proliferation and apoptosis and its potential prediction of clinical prognosis of HCC patients. (springer.com)
  • nor did it affect the maintenance or regulation of the stem cells. (elifesciences.org)
  • They include antiproliferation, antiangiogenesis, proapoptosis, prodifferentiation, and immune regulation in a cell- and tissue- specific manner [ 5 - 9 ]. (hindawi.com)
  • The CKIs p27 Kip1 and p21 Cip1 inactivate the cyclin-CDK complexes in the G 1 phase leading to cell cycle arrest, and thus function in growth regulation and wound repair. (ahajournals.org)
  • By elucidating the REST-p21 genetic mechanism underlying the cell cycle regulation of proliferating cardiomyocytes during cardiac development and regeneration, our study provides an opportunity for developing cell-based therapeutics for heart disease. (nature.com)
  • Migratory DCs in the periphery, lymphatic, and nonlymphatic organs then undergo phenotypic and functional changes, including up-regulation of cell surface expression of costimulatory and adhesion molecules and production of inflammatory chemokines and cytokines ( 12 - 15 ). (pnas.org)
  • These cells also efficiently enhanced skin wound healing in vivo, in a manner comparable to that of hWJ-MSC obtained from 2D culture. (biomedcentral.com)
  • Our previous studies have shown that HHcy accelerates early atherosclerotic lesion formation in apolipoprotein E-deficient (ApoE −/− ) mice and that Hcy stimulation in vitro and ex vivo can induce B cell proliferation and IgG Ab secretion ( 3 - 5 ). (jimmunol.org)
  • Global conditional deletion of Angpt1 , or deletion from osteoblasts, LepR + cells, Nes-cre -expressing cells, megakaryocytes, endothelial cells or hematopoietic cells in normal mice did not affect hematopoiesis, HSC maintenance, or HSC quiescence. (elifesciences.org)
  • and, in these mice, blood stem cells and blood cell production recovered more quickly than in mice with Angpt1. (elifesciences.org)
  • Despite the increased β-cell mass, islets from mice lacking Foxo1 in pancreatic or endocrine progenitors responded poorly to glucose, resulting in glucose intolerance. (diabetesjournals.org)
  • Accordingly, β-cell mass continues to expand in mice lacking Foxo1 in endocrine progenitors, but cells do not function properly, impairing insulin secretion. (diabetesjournals.org)
  • In this study, we examined mature T cells from spleen and lymph nodes of tamoxifen-inducible CAML knockout mice ( tCAML −/− ). (jimmunol.org)
  • Unless stated otherwise, the peripheral mature T cells used in this study were obtained by negative enrichment of CD4 + and CD8 + T cells from spleen and lymph nodes of control ( ESRCre − CAML fl/fl , hereafter referred to as control) and experimental ( ESRCre + CAML fl/fl designated as tamoxifen-induced CAML deletion [ tCAML − / − ]) mice. (jimmunol.org)
  • Further exploiting the findings, we show that putative mitogen-activated protein-kinases contribute to the melarsoprol-induced mitotic defect, reminiscent of the mitotic arrest associated signalling cascade triggered by arsenicals in mammalian cells, used to treat leukaemia. (classicistranieri.com)
  • Mechanistically, Hcy increased both the protein expression and glycolytic enzyme activity of the pyruvate kinase muscle isozyme 2 (PKM2) in B cells, whereas the PKM2 inhibitor shikonin restored Hcy-induced metabolic changes, as well as B cell proliferation and Ab secretion both in vivo and in vitro, indicating that PKM2 plays a critical role in metabolic reprogramming in Hcy-activated B cells. (jimmunol.org)
  • These experiments showed where the protein is produced, and unexpectedly revealed that the bone cells do not make Angpt1. (elifesciences.org)
  • Instead, it is the stem cells and the stromal cells in the niche that made the protein. (elifesciences.org)
  • During G 2 -phase, the level of phosphorylated Aurora-A at centrosomes is reduced in hyaluronan-mediated motility receptor (HMMR)-silenced cells as is the nuclear transport of TPX2, an Aurora-A-activating protein. (aacrjournals.org)
  • Lentivirus vector‑interleukin‑10 green fluorescent protein (LV‑IL‑10‑GFP) was transfected into endothelial progenitor cells (EPCs) in the present study. (spandidos-publications.com)
  • SUMOylation is a critical regulatory protein modification in eukaryotic cells and plays a pivotal role in cardiac development and disease. (chemweb.com)
  • In the present study, the dose-dependent effects of pirfenidone on the cell cycle, proliferation and expression of heat shock protein (HSP)-47 and collagen type I in a cultured rat hepatic stellate cell line (HSC-T6) were investigated. (sigmaaldrich.com)
  • However, after 24 h, HSC-T6 cells exhibited dose-dependent decreases in HSP-47 protein and collagen I mRNA levels. (sigmaaldrich.com)
  • The expression of checkpoint kinase 2 (CHK2), cell division control protein 2 (CDC2), and cyclin B1 was investigated using qRT-PCR and Western blot analysis. (medsci.org)
  • β-adrenoceptors are members of the superfamily of G protein-coupled adrenergic receptors, which mediate actions of the endogenous catecholamines in a variety of target cells [ 10 , 11 ]. (biomedcentral.com)
  • Whether a cell undergoes apoptosis or continues through the cell cycle in response to potential apoptotic stimuli is under the control of a complex set of gene and protein interactions. (aacrjournals.org)
  • Protein expression was detected by Western blot analysis with chemiluminescent imaging. (gsu.edu)
  • The cells in G1Q are quiescent, temporarily withdrawn from the cell cycle (also identifiable as G0), the G1A are in the growth phase while G1B are the cells just prior entering S, with their growth (RNA and protein content, size) similar to that of the cells initiating DNA replication. (wikipedia.org)
  • Foxo1 is important to maintain adult β-cells, but little is known about its role in pancreatic progenitor cells as determinants of future β-cell function. (diabetesjournals.org)
  • Surprisingly, ablation of Foxo1 in pancreatic progenitors resulted in delayed appearance of Neurogenin3 + progenitors and their persistence into adulthood as a self-replicating pool, causing a fourfold increase of β-cell mass. (diabetesjournals.org)
  • The microarray results were validated by qRT-PCR in PDAC tissues, paired adjacent normal pancreatic tissues, PDAC cell lines, and a normal pancreas cell line. (ijbs.com)
  • Hematopoietic stem/progenitor cells and LepR + stromal cells regulate niche regeneration by secreting Angpt1, reducing vascular leakiness but slowing niche recovery. (elifesciences.org)
  • Bone marrow (BM)-derived endothelial progenitor cells (EPCs) play a crucial role in neovascularazation. (spandidos-publications.com)
  • Six classes of neurons and Müller glial cells are generated from a common retinal progenitor cell (RPC) population, following a precise temporal sequence ( Livesey and Cepko, 2001 ). (biologists.org)
  • We conclude that Foxo1 integrates cues that determine developmental timing, pool size, and functional features of endocrine progenitor cells, resulting in a legacy effect on adult β-cell mass and function. (diabetesjournals.org)
  • The surprising results of this investigation indicate that Foxo1 controls timing and size of the endocrine progenitor cell pool, such that embryonic Foxo1 ablation delays formation of endocrine progenitors and causes their persistence into adulthood ( 12 ). (diabetesjournals.org)
  • Mesenchymal stem cells (MSCs) are multipotent cells that can be induced to differentiate into a variety of mesenchymal tissues, including bone, cartilage, fat, bone marrow stroma, and muscle [ 1 - 3 ]. (plos.org)
  • The role of miR-27a in tumorigenesis differs in various cells and tissues. (biomedcentral.com)
  • Among these tissues and organs, Wharton's jelly from the human umbilical cord is readily available, easily harvested, and is a rich source of human Wharton's jelly-derived mesenchymal stem cells (hWJ-MSC). (biomedcentral.com)
  • Further mechanistic analysis showed that CASC15 epigenetically silenced the expression of programmed cell death 4 (PDCD4) by recruiting EZH2 and increasing H3K27me3 level at the promoter region of PDCD4. (springer.com)
  • Combining cell-cycle checkpoint kinase inhibitors with the DNA-damaging chemotherapeutic agent gemcitabine offers clinical appeal, with a mechanistic rationale based chiefly on abrogation of gemcitabine-induced G 2 -M checkpoint activation. (aacrjournals.org)
  • Mechanistic analyses indicated that DUXAP8 regulates PC cell proliferation partly through downregulation of tumor suppressor CDKN1A and KLF2 expression. (biomedcentral.com)
  • Mechanistic investigations revealed that the oncogenic effect of MARCH5 was mainly mediated by increased mitochondrial fission and subsequent ROS production in BC cells. (dovepress.com)
  • Placenta-specific 8 (PLAC8, Onzin) was first identified during a genome-wide analysis of gene expression in the placenta, where its expression was restricted to the spongiotrophoblast layer of the mouse placenta and was therefore called PLAC8 3 , 4 . (nature.com)
  • Caki-1, a metastatic RCC cell line, showed higher expression of molecular EMT markers, including Snail and Zeb1, but decreased activity of GSK3β. (aacrjournals.org)
  • However, we observe that YTHDF1 high expression correlates with better clinical outcome, with its depletion rendering cancerous cells resistant to cisplatin (DDP) treatment. (nih.gov)
  • 2) The lentivirus infection was used to silence the expression of HNF4α in SGC7901 cell. (frontiersin.org)
  • The RNA regulators PUMILIO sustain HSPC and acute myeloid leukemia cell growth by upregulating FOXP1 expression through direct binding to 2 FOXP1 -3′UTR PUMILIO-binding elements. (bloodjournal.org)
  • FOXP1 mediates PUMILIO growth-promoting activities by repressing expression of p21 CIP1 and p27 KIP1 cell cycle inhibitors. (bloodjournal.org)
  • Expression of FOXP1 strongly correlates with PUM1 and PUM2 levels in primary HSPCs and myeloid leukemia cells. (bloodjournal.org)
  • Mechanistically, FOXP1 represses the expression of the p21 −CIP1 and p27 −KIP1 cell cycle inhibitors. (bloodjournal.org)
  • Gene expression has demonstrated different patterns of expression in endothelial cells extracted from tumor, implying bilateral cross-talk [ 3 ]. (biomedcentral.com)
  • In conclusion, pirfenidone inhibited HSC-T6 cell proliferation, arrested the cell cycle and reduced the expression of HSP-47 and collagen type I, indicating that pirfenidone may be a promising drug in the treatment of liver fibrosis. (sigmaaldrich.com)
  • RAD51 expression was observed broadly and was not associated with PTEN status in the 16 cell lines. (biomedcentral.com)
  • The expression level of nuclear PTEN was not elevated within 24 h following IR in the HEC-6-PTEN + cells. (biomedcentral.com)
  • The impact of HCV polyprotein expression in human cells has been hampered by limitations of different cell systems to express the entire HCV polyprotein in high yields and in all cells. (biomedcentral.com)
  • As it has been considered that the viral cytopathic effect might be involved in the liver-cell injuries [ 1 , 2 , 13 ], here we have analyzed in detail the subcellular forms and biochemical changes occurring in human cells (HeLa and hepatic HepG2) following expression of the HCV polyprotein from VACV recombinant. (biomedcentral.com)
  • In contrast, ablation of Foxo1 in terminally differentiated β-cells did not increase β-cell mass nor did it affect Neurogenin3 expression. (diabetesjournals.org)
  • In the adult pancreas, Foxo1 expression is restricted to β-cells ( 6 , 7 ), where it modulates metabolic flexibility (i.e., the ability to switch between glucose and lipids as a source of acyl-CoA for mitochondrial oxidative phosphorylation [ 8 ]) and enforces the β-cell fate, allowing β-cells to retain terminally differentiated features ( 9 ). (diabetesjournals.org)
  • The expression of YAP in CML cells was determined by western blotting. (biomedcentral.com)
  • In addition, expression of lncRNAs (PEG10, LUNAR1 and HULC) is correlated with clinical poor prognosis in diffuse large B cell lymphoma (DLBCL). (biomedcentral.com)
  • In this study, we investigated aberrantly expressed miRNAs involved in PDAC by comparing miRNA expression profiles in PDAC cell lines with a normal pancreas cell line and found that miR-135a was significantly down-regulated in the PDAC cell lines. (ijbs.com)
  • Similar cell cycle compartments are also recognized by multiparameter analysis that includes measurement of expression of cyclin D1, cyclin E, cyclin A and cyclin B1, each in relation to DNA content Concurrent measurement of DNA content and of incorporation of DNA precursor 5-bromo-2'-deoxyuridine (BrdU) by flow cytometry is an especially useful assay, that has been widely used in analysis of the cell cycle in vitro and in vivo. (wikipedia.org)
  • Angpt1 is known to regulate blood vessels in the bone marrow, and one influential study had previously suggested that bone cells produce Angpt1, which promotes and regulates the maintenance of the stem cells within the niche. (elifesciences.org)
  • Thus, it was not clear whether Angpt1 promotes the regeneration of the stem cells themselves or if it regulates the rebuilding of the niche. (elifesciences.org)
  • Here we show that REST also regulates the cardiomyocyte cell cycle. (nature.com)
  • However, high prices of automated cell cycle analysis instruments, and reluctance among researchers to use advanced cell cycle analysis techniques may restrict the growth of this market. (marketresearchfuture.com)
  • Also, reluctance among researchers to use advanced cell cycle analysis techniques is another growth barrier for the market. (marketresearchfuture.com)
  • Cell cycle analysis is often used for the screening of compounds that affect cell growth and proliferation. (nexcelom.com)
  • In this study, we showed that Hcy-activated B cells displayed an increase in both oxidative phosphorylation and glycolysis, with a tendency to shift toward the latter, as well as an accumulation of intermediates in the pentose phosphate pathway, to provide energy and biosynthetic substrates for cell growth and function. (jimmunol.org)
  • We demonstrate that FOXP1 by itself supports HSPC and leukemic cell growth, thus mimicking PUM activities. (bloodjournal.org)
  • We demonstrate that MART-10 is 500- to 1000-fold more potent than 1 α ,25(OH) 2 D 3 in inhibiting cell growth in a dose- and time-dependent manner. (hindawi.com)
  • The Kip/Cip CKIs, p27 Kip1 and p21 Cip1 , are upregulated during arterial repair and negatively regulate the growth of vascular smooth muscle cells (VSMCs). (ahajournals.org)
  • We hypothesized that a variation in the inactivation of cdk2 and cdk4 during the G 1 phase of the cell cycle by p27 Kip1 , p21 Cip1 , and p16 Ink4 leads to different effects on VSMC growth in vitro and in vivo. (ahajournals.org)
  • In this context, evidence suggests that endothelial cells (EC) play an important role in ovarian tumor growth and chemoresistance. (biomedcentral.com)
  • The significance of endothelial cells (ECs) in this scenario was initially attributed to their role in neo-angiogenesis that in fact is critical for tumor initiation and growth. (biomedcentral.com)
  • Cells were cultured in endothelial cell growth medium (Medium 199, 20% (v/v) fetal bovine serum (FBS), 20 μg ml-1 endothelial cell growth supplement (Hallway), 1% (v/v) antibiotics (Hallway), and 20 units ml-1 heparin). (biomedcentral.com)
  • Suppression of WNT5B remarkably impaired cell growth, migration and mammosphere formation. (sigmaaldrich.com)
  • Further growth of the heart occurs only by hypertrophy of the preexisting heart cells ( 24 , 26 ). (physiology.org)
  • Epidermal growth factor (EGF) receptor (EGFR), a receptor tyrosine kinase that belongs to the family of ErbB receptors, has been implicated in promoting proliferation in many cell types including vascular smooth muscle cells ( 23 ) and fetal cardiomyocytes ( 16 , 41 ). (physiology.org)
  • By employing soft agar assays and tumor xenograft models, we showed that uc.345 could accelerate tumor growth. (oncotarget.com)
  • Our previous studies demonstrated that, in other cell types, galangin is a potent inhibitor of the aryl hydrocarbon receptor (AhR), an environmental carcinogen-responsive transcription factor implicated in mammary tumor initiation and growth control. (biomedcentral.com)
  • Galangin inhibited transition of cells from the G 0 /G 1 to the S phases of cell growth, likely through the nearly total elimination of cyclin D3. (biomedcentral.com)
  • NR4A2 is a nuclear receptor belonging to the NR4A subfamily and vital in regulating cell growth, metabolism, inflammation and other biological functions. (peerj.com)
  • PEEP inhibited the growth of HeLa cells, and the optimum concentration of PEEP was 150 mg/ml. (biomedcentral.com)
  • In vivo, CTAB ablated tumor-forming capacity of FaDu cells and delayed growth of established tumors. (aspetjournals.org)
  • Thus, identification of novel molecular mechanisms leading to metastatic growth of GC cells is critical for the development of new therapeutic targets for treatment of this malignancy. (dovepress.com)
  • Results revealed that Bakuchiol exerted potent, dose-dependent as well as time-dependent growth inhibitory effects in SGC-7901 cell proliferation with IC50 values of 58.4, 42.3 and 32.5 μM at 12, 24 and 48 h time intervals respectively. (alliedacademies.org)
  • An arrest of cells in G0 or G1 is often seen as a result of lack of nutrients (growth factors), for example after serum deprivation. (wikipedia.org)
  • Studies that examine the growth rate of microorganisms (in other words: how fast they divide to create new cells) require cell counting. (wikipedia.org)
  • To quantify the number of cells in a culture, the cells can be simply plated on a petri dish with growth medium. (wikipedia.org)
  • In conclusion, our results demonstrate that PKM2 is required to support metabolic reprogramming for Hcy-induced B cell activation and function, and it might serve as a critical regulator in HHcy-accelerated initiation of atherosclerosis. (jimmunol.org)
  • Aberrant activation of WNT signaling has been detected in TNBC, which might be important for triggering oncogenic conversion of breast epithelial cell. (sigmaaldrich.com)
  • uc.158− was overexpressed in human HepG2 versus Huh7 cells in line with activation of the Wnt pathway. (bmj.com)
  • Whereas CAML-deficient T cells were able to express the early activation markers CD25 and CD69, and produce IL-2 normally upon stimulation, deficient cells proliferated less and died. (jimmunol.org)
  • Responsiveness to TCR stimulation is critical for T cells to carry out effector functions during Ag recognition and activation. (jimmunol.org)
  • Therefore, it is critically important to understand the components that are required to sustain TCR-dependent activation and that support optimum T cell function ( 5 - 7 ). (jimmunol.org)
  • We find that CAML is required for T cells to proliferate upon receiving mitogenic signals, but is dispensable for early activation events. (jimmunol.org)
  • Additionally, the β 2 -adrenergic antagonist reduced the activation of NFκB in vitro cultured PanCa cells. (biomedcentral.com)
  • This increase resulted in concomitant transactivation of the EGF receptor and sustained activation of extracellular signal related kinase (ERK) 1/2, culminating in enhanced cell proliferation. (rupress.org)
  • Several studies have demonstrated that BM-mononuclear cells (MNCs), as well as mesenchymal stem cells (MSCs), have the ability to immunoregulate and improve tissue repair through IL-10 secretion ( 9 - 11 ). (spandidos-publications.com)
  • Here, we investigate the interactions of 2D nanosilicates, a layered clay, with human mesenchymal stem cells (hMSCs) at the whole-transcriptome level by high-throughput sequencing (RNA-seq). (pnas.org)