Methods for maintaining or growing CELLS in vitro.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Established cell cultures that have the potential to propagate indefinitely.
Process of growing viruses in live animals, plants, or cultured cells.
Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
The initial culturing of cells derived directly from fresh TISSUES.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
Elements of limited time intervals, contributing to particular results or situations.
A CELL LINE derived from the kidney of the African green (vervet) monkey, (CERCOPITHECUS AETHIOPS) used primarily in virus replication studies and plaque assays.
A cell line derived from cultured tumor cells.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Inoculation of a series of animals or in vitro tissue with an infectious bacterium or virus, as in VIRULENCE studies and the development of vaccines.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Ribonucleic acid that makes up the genetic material of viruses.
A suborder of PRIMATES consisting of six families: CEBIDAE (some New World monkeys), ATELIDAE (some New World monkeys), CERCOPITHECIDAE (Old World monkeys), HYLOBATIDAE (gibbons and siamangs), CALLITRICHINAE (marmosets and tamarins), and HOMINIDAE (humans and great apes).
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The rate dynamics in chemical or physical systems.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly.
A genus of the family PICORNAVIRIDAE whose members preferentially inhabit the intestinal tract of a variety of hosts. The genus contains many species. Newly described members of human enteroviruses are assigned continuous numbers with the species designated "human enterovirus".
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.
Substances elaborated by viruses that have antigenic activity.
A genus of PICORNAVIRIDAE causing infectious hepatitis naturally in humans and experimentally in other primates. It is transmitted through fecal contamination of food or water. HEPATITIS A VIRUS is the type species.
Silicone polymers which consist of silicon atoms substituted with methyl groups and linked by oxygen atoms. They comprise a series of biocompatible materials used as liquids, gels or solids; as film for artificial membranes, gels for implants, and liquids for drug vehicles; and as antifoaming agents.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
The study of the structure, growth, function, genetics, and reproduction of viruses, and VIRUS DISEASES.
Methods used to study CELLS.
Proteins prepared by recombinant DNA technology.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
Deoxyribonucleic acid that makes up the genetic material of viruses.
One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.
Proteins found in any species of virus.
Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Minute infectious agents whose genomes are composed of DNA or RNA, but not both. They are characterized by a lack of independent metabolism and the inability to replicate outside living host cells.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells.
The complete genetic complement contained in a DNA or RNA molecule in a virus.
A family of enveloped, linear, double-stranded DNA viruses infecting a wide variety of animals. Subfamilies, based on biological characteristics, include: ALPHAHERPESVIRINAE; BETAHERPESVIRINAE; and GAMMAHERPESVIRINAE.
Type species of CHLAMYDIA causing a variety of ocular and urogenital diseases.
An area showing altered staining behavior in the nucleus or cytoplasm of a virus-infected cell. Some inclusion bodies represent "virus factories" in which viral nucleic acid or protein is being synthesized; others are merely artifacts of fixation and staining. One example, Negri bodies, are found in the cytoplasm or processes of nerve cells in animals that have died from rabies.
The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.
Adherence of cells to surfaces or to other cells.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Antibodies produced by a single clone of cells.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
A genus of gram-negative, mostly facultatively anaerobic bacteria in the family MYCOPLASMATACEAE. The cells are bounded by a PLASMA MEMBRANE and lack a true CELL WALL. Its organisms are pathogens found on the MUCOUS MEMBRANES of humans, ANIMALS, and BIRDS.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Proteins secreted by vertebrate cells in response to a wide variety of inducers. They confer resistance against many different viruses, inhibit proliferation of normal and malignant cells, impede multiplication of intracellular parasites, enhance macrophage and granulocyte phagocytosis, augment natural killer cell activity, and show several other immunomodulatory functions.
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
Refers to animals in the period of time just after birth.
Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Tools or devices for generating products using the synthetic or chemical conversion capacity of a biological system. They can be classical fermentors, cell culture perfusion systems, or enzyme bioreactors. For production of proteins or enzymes, recombinant microorganisms such as bacteria, mammalian cells, or insect or plant cells are usually chosen.
Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Infections with bacteria of the genus CHLAMYDIA.
Water swollen, rigid, 3-dimensional network of cross-linked, hydrophilic macromolecules, 20-95% water. They are used in paints, printing inks, foodstuffs, pharmaceuticals, and cosmetics. (Grant & Hackh's Chemical Dictionary, 5th ed)
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Spherical, heterogeneous aggregates of proliferating, quiescent, and necrotic cells in culture that retain three-dimensional architecture and tissue-specific functions. The ability to form spheroids is a characteristic trait of CULTURED TUMOR CELLS derived from solid TUMORS. Cells from normal tissues can also form spheroids. They represent an in-vitro model for studies of the biology of both normal and malignant cells. (From Bjerkvig, Spheroid Culture in Cancer Research, 1992, p4)
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.
The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells, such as ENTEROCYTES. These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation.
An encapsulated lymphatic organ through which venous blood filters.
A genus of FLAVIVIRIDAE causing parenterally-transmitted HEPATITIS C which is associated with transfusions and drug abuse. Hepatitis C virus is the type species.
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Proteins encoded by a VIRAL GENOME that are produced in the organisms they infect, but not packaged into the VIRUS PARTICLES. Some of these proteins may play roles within the infected cell during VIRUS REPLICATION or act in regulation of virus replication or VIRUS ASSEMBLY.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
Arthropod-borne viruses. A non-taxonomic designation for viruses that can replicate in both vertebrate hosts and arthropod vectors. Included are some members of the following families: ARENAVIRIDAE; BUNYAVIRIDAE; REOVIRIDAE; TOGAVIRIDAE; and FLAVIVIRIDAE. (From Dictionary of Microbiology and Molecular Biology, 2nd ed)
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.
A genus of the family HERPESVIRIDAE, subfamily ALPHAHERPESVIRINAE, consisting of herpes simplex-like viruses. The type species is HERPESVIRUS 1, HUMAN.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Methods for cultivation of cells, usually on a large-scale, in a closed system for the purpose of producing cells or cellular products to harvest.
Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.
A family of unenveloped RNA viruses with cubic symmetry. The twelve genera include ORTHOREOVIRUS; ORBIVIRUS; COLTIVIRUS; ROTAVIRUS; Aquareovirus, Cypovirus, Phytoreovirus, Fijivirus, Seadornavirus, Idnoreovirus, Mycoreovirus, and Oryzavirus.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.
The non-neuronal cells of the nervous system. They not only provide physical support, but also respond to injury, regulate the ionic and chemical composition of the extracellular milieu, participate in the BLOOD-BRAIN BARRIER and BLOOD-RETINAL BARRIER, form the myelin insulation of nervous pathways, guide neuronal migration during development, and exchange metabolites with neurons. Neuroglia have high-affinity transmitter uptake systems, voltage-dependent and transmitter-gated ion channels, and can release transmitters, but their role in signaling (as in many other functions) is unclear.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Preclinical testing of drugs in experimental animals or in vitro for their biological and toxic effects and potential clinical applications.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
A genus of REOVIRIDAE, causing acute gastroenteritis in BIRDS and MAMMALS, including humans. Transmission is horizontal and by environmental contamination. Seven species (Rotaviruses A thru G) are recognized.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
A tube that transports URINE from the URINARY BLADDER to the outside of the body in both the sexes. It also has a reproductive function in the male by providing a passage for SPERM.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Viruses whose taxonomic relationships have not been established.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
A genus of the family CHLAMYDIACEAE whose species cause a variety of diseases in vertebrates including humans, mice, and swine. Chlamydia species are gram-negative and produce glycogen. The type species is CHLAMYDIA TRACHOMATIS.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
A form of interference microscopy in which variations of the refracting index in the object are converted into variations of intensity in the image. This is achieved by the action of a phase plate.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
A species in the genus HEPATOVIRUS containing one serotype and two strains: HUMAN HEPATITIS A VIRUS and Simian hepatitis A virus causing hepatitis in humans (HEPATITIS A) and primates, respectively.
The functional hereditary units of VIRUSES.
A genus of Eurasian herbaceous plants, the poppies (family PAPAVERACEAE of the dicotyledon class Magnoliopsida), that yield OPIUM from the latex of the unripe seed pods.
A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.
Viruses whose genetic material is RNA.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Techniques used in studying bacteria.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
The protein complement of an organism coded for by its genome.
A genus of the family Muridae consisting of eleven species. C. migratorius, the grey or Armenian hamster, and C. griseus, the Chinese hamster, are the two species used in biomedical research.
Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).
A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)
A species of ENTEROVIRUS which is the causal agent of POLIOMYELITIS in humans. Three serotypes (strains) exist. Transmission is by the fecal-oral route, pharyngeal secretions, or mechanical vector (flies). Vaccines with both inactivated and live attenuated virus have proven effective in immunizing against the infection.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
A genus of the family PARAMYXOVIRIDAE (subfamily PARAMYXOVIRINAE) where all the virions have both HEMAGGLUTININ and NEURAMINIDASE activities and encode a non-structural C protein. SENDAI VIRUS is the type species.
The sum of the weight of all the atoms in a molecule.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
The innermost membranous sac that surrounds and protects the developing embryo which is bathed in the AMNIOTIC FLUID. Amnion cells are secretory EPITHELIAL CELLS and contribute to the amniotic fluid.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Cell culture[edit]. Puromycin is used in cell biology as a selective agent in cell culture systems. It is toxic to prokaryotic ... Puromycin is stable for one year as solution when stored at -20 °C. The recommended dose as a selection agent in cell cultures ... "Mol Biol Cell. 8 (8): 1559-1573. doi:10.1091/mbc.8.8.1559. PMC 276176. PMID 9285825.. ... "In Hopsu-Havu, Väinö K.; Järvinen, Mikko; Kirschke, Heidrun (eds.). Proteolysis in Cell Functions. IOS Press. pp. 88-95. ISBN ...
Cell culture[edit]. Mineral oil of special purity is often used as an overlay covering microdrops of culture medium in petri ... The use of oil presents several advantages over the open culture system: it allows for several oocytes and embryos to be ... as an adjuvant to stimulate a cell-mediated immune response to the vaccinating agent.[citation needed] In the poultry industry ... as few as 20 microlitres per oocyte instead of several millilitres for the batch culture); and it serves as a temperature ...
... mammalian cell cultures; vegetables; human nostrils and throats; and human and animal brain, skin, and lung tissues. B. ...
Cell culture. *Model organisms (such as C57BL/6 mice). *Methods *Nucleic acid ... Up-regulation is a process that occurs within a cell triggered by a signal (originating internal or external to the cell), ... Cell. 62 (5): 712-27. doi:10.1016/j.molcel.2016.04.006. PMC 5476208 . PMID 27259203.. ... Cells do this by modulating the capping, splicing, addition of a Poly(A) Tail, the sequence-specific nuclear export rates, and ...
Inhibitory effect on cultures tumor cell 5 Erythropoietin Kidney Stimulate development of erythropoietic cells ... Stimulate growth of mesenchymal cells, promotes wound healing 3 Transforming growth factor (Alpha) Epithelial cell Similar to ... cell membrane. Biological process. • negative regulation of epidermal growth factor receptor signaling pathway. • positive ... regulation of cell motility. • activation of MAPKK activity. • positive regulation of receptor internalization. • positive ...
August 2002). "Antiproliferative action of valorphin in cell cultures". Journal of Peptide Science. 8 (8): 438-52. doi:10.1002/ ... co-relations between amino acid sequences and effects in cell cultures". Peptides. 23 (5): 903-10. doi:10.1016/S0196-9781(02) ... It also possesses cytotoxic and antiproliferative properties against tumor cells,[3][4][5][6] the mediation of which, because ... "Tumor cell cytolysis mediated by valorphin, an opioid-like fragment of hemoglobin beta-chain". Peptides. 18 (1): 79-85. doi ...
An incubator is a device used to grow and maintain microbiological cultures or cell cultures. The incubator maintains optimal ... "Improvements in CO2 Incubators for Cell Cultures". Retrieved 2019-12-02.. ... They began to experiment to find the ideal environment for maintaining cell culture stocks. These early incubators were simply ... microbiology and molecular biology and are used to culture both bacterial and eukaryotic cells. ...
Phosphate buffered saline (cell culture). *Tyrode's solution. References[edit]. *^ a b c British national formulary: BNF 69 (69 ...
Narayanaswamy, S. (1994). Plant Cell and Tissue Culture. Tata McGraw-Hill Education. pp. vi. ISBN 978-0-07-460277-5. .. ... Embryonic stem cells incorporate the altered gene, which replaces the already present functional copy. These stem cells are ... As only a single cell is transformed with genetic material, the organism must be regenerated from that single cell. In plants ... DNA is generally inserted into animal cells using microinjection, where it can be injected through the cell's nuclear envelope ...
Ader M., Tanaka E. M. (2014). "Modeling human development in 3D culture". Current Opinion in Cell Biology. 31: 23-28. doi: ... This occurs when individual cells or groups of cells grow longer. Not all plant cells will grow to the same length. When cells ... These cell divisions are usually rapid with no growth so the daughter cells are half the size of the mother cell and the whole ... A single tissue, formed from a single type of progenitor cell or stem cell, often consists of several differentiated cell types ...
"Labeling Cultured Cells with32Piand Preparing Cell Lysates for Immunoprecipitation". Labeling Cultured Cells with 32Pi and ... ACK is used for lysis of red blood cells in biological samples where other cells such as white blood cells are of greater ... "Lysis of Cultured Cells for Immunoprecipitation". Cold Spring Harbor Protocols. 2010 (8): pdb.prot5466. doi:10.1101/pdb. ... "Detergents for Cell Lysis and Protein Extraction". Retrieved 2016-03-16.. ...
Predieri, S. (2001). "Mutation induction and tissue culture in improving fruits". Plant Cell, Tissue and Organ Culture. 64 (2/3 ... Electroporation is used when the plant tissue does not contain cell walls. In this technique, "DNA enters the plant cells ... DNA is bound to tiny particles of gold or tungsten which are subsequently shot into plant tissue or single plant cells under ... The accelerated particles penetrate both the cell wall and membranes. The DNA separates from the metal and is integrated into ...
"Plant Cell, Tissue and Organ Culture. 72 (1): 95-98. doi:10.1023/A:1021203811457. Retrieved 19 May 2016.. ... "The Plant Cell. 14 (suppl 1): S153-S164. doi:10.1105/tpc.000679. ISSN 1532-298X. PMC 151253 . PMID 12045275. Retrieved 26 May ... Induces Apoptosis and Cell Cycle Arrest in A549 Cells through p53 Accumulation via c-Jun NH2-Terminal Kinase-Mediated ... Venus flytraps can also be propagated in vitro using plant tissue culture.[38] Most Venus flytraps found for sale in nurseries ...
Cell cultureEdit. Petri dishes are also used for cell cultivation of isolated cells from eukaryotic organisms, such as in ... alternatively known as a Petri plate or cell-culture dish) is a shallow transparent lidded dish that biologists use to culture ... Axenic Cell culture of the plant Physcomitrella patens on an agarplate in a Petri dish ... In the latter, the cells often grow as a layer attached to the bottom surface of the dish, below the culture medium.[25] ...
... cells are most commonly used not as individual cells, but as a confluent monolayer on a cell culture insert filter (e.g ... 2005). "The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell ... 1983). "Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture". Biol Cell. ... When looking at Caco-2 cell cultures microscopically, it is evident even by visual inspection that the cells are heterogeneous ...
G. Diets, Culture Media, Food Supplements. Vol. 4. Culture Media for Cells, Organs and Embryos. Cleveland, OH: CRC Press, 1977 ... G. Diets, Culture Media, Food Supplements. Vol. 3. Culture Media for Microorganisms and Plants. Cleveland, OH: CRC Press, 1978 ... G. Diets, Culture Media, Food Supplements. Vol. 1. Dits for Mammals. Cleveland, OH: CRC Press, 1977. 645 pp. CRC Handbook ... G. Diets, Culture Media, Food Supplements. Vol. 2. Food Habits of and Diets for Invertebrates and Vertebrates. Zoo diets. ...
Lee SY (1996). "High cell-density culture of Escherichia coli". Trends Biotechnol. 14 (3): 98-105. doi:10.1016/0167-7799(96) ... Because of its long history of laboratory culture and ease of manipulation, E. coli also plays an important role in modern ... Due to its ease of culture and fast doubling, it was used in the early microbiology experiments; however, bacteria were ... "Microbial Cell Factories. 9 (61): 494-7. doi:10.1186/1475-2859-9-61. PMC 2927510. PMID 20701771.. ...
cell suspension cultures". Plant Physiology. 133 (3): 1306-13. doi:10.1104/pp.103.025213. PMC 281625. PMID 14551337. Biology ... the crucial cytochrome P450 mono-oxygenase for lavandulylated flavanone formation in Sophora flavescens cultured cells". ...
cell suspension cultures". Phytochemistry. 53 (5): 533-8. doi:10.1016/s0031-9422(99)00593-2. PMID 10724177. Baicalein+7-O- ...
cell suspension cultures". Plant Physiology. 133 (3): 1306-13. doi:10.1104/pp.103.025213. PMC 281625. PMID 14551337. - ...
Evidence from cell lines and primary breast cancer cultures in vitro". Angiogenesis. 8 (3): 197-204. doi:10.1007/s10456-005- ... Paracrine signaling is a form of cell-cell communication in which a cell produces a signal to induce changes in nearby cells, ... These T cells can then go on to perform effector functions such as macrophage activation, B cell activation, and cell-mediated ... When interleukin-1 is produced in response to external stimuli, it can bind to cell-surface receptors on the same cell that ...
Lyophilized cell cultures of Trametes hirsuta in the presence of oxygen.[18] ...
Kampf C; Roomans GM (May 2001). "Effects of hypochlorite on cultured respiratory epithelial cells". Free Radic. Res. 34 (5): ... Nitric oxide can diffuse through the plasma membrane into neighbouring cells, allowing cell signalling, so nitroindazole ...
"BRCA1 inhibition of telomerase activity in cultured cells". Molecular and Cellular Biology. 23 (23): 8668-90. PMC 262673 . PMID ... cell cycle arrest. • positive regulation of response to DNA damage stimulus. • positive regulation of cell proliferation. • ... "Cell. 142 (3): 480-93. PMC 2923036 . PMID 20691906. doi:10.1016/j.cell.2010.06.037.. ... "Cell. 151 (1): 68-79. PMC 3471363 . PMID 23021216. doi:10.1016/j.cell.2012.08.033.. ...
Commonly used cell lines are Chinese hamster ovary (CHO) cells or plant cell cultures. The production volumes are very small. ... When carried out outside the natural habitat, the process is called cell culture. Mammalian cell culture fermentation, also ... Cell Cultures Animal or plant cells, removed from tissues, will continue to grow if cultivated under the appropriate nutrients ... Handbook of Industrial Cell Culture: Mammalian, Microbial, and Plant Cells. Humana Press, New York. C. Chassin; P. Pollak ( ...
In lymphoblastoid cell cultures, Syk tyrosine kinases have been found in LMP2A immunoprecipitates following in vitro kinase ... These messages are expressed in Epstein-Barr Virus transformed lymphoblastoid cell cultures. The ratio of these messages varies ... This results in BCR-negative cells being able to exit the bone marrow and survive in peripheral lymphoid organs. B-cells from ... LMP2A is a transmembrane protein that inhibits normal B-cell signal transduction by mimicking an activated B-cell receptor (BCR ...
Cell culture: Human or animal cell cultures are infected with the microbe of interest. These cultures are then observed to ... These are primarily used to culture bacteria and fungi.. *Liquid culture: Cells are grown inside a liquid media. Microbial ... Once the virus has gained access to the host's cells, the virus' genetic material (RNA or DNA) must be introduced to the cell. ... Additionally, the extracellular matrix and dense outer layer of bacterial cells can protect the inner bacteria cells from ...
Electron micrograph of Hepacivirus C purified from cell culture. Scale bar = 50 nanometres ... Follicular dendritic cell sarcoma. Extranodal NK/T-cell lymphoma, nasal type. MCPyV Merkel-cell carcinoma. RNA virus. HCV ... is a root extract found in the continent of Asia that has been reported to have antiviral activity against HCV in cell cultures ... Study of multiple genetic variations caused by persistent hepatitis C virus replication in long-term cell culture. Arch Virol ...
For spheroids in cell culturing, see 3D cell culture. For rotating equilibrium spheroids, see Maclaurin spheroid. ...
... which has been found in one study to be mutagenic and genotoxic in cell cultures which do not respond to β-carotene itself.[30] ... "Toxicity of oxidized β-carotene to cultured human cells". Experimental Eye Research. 81 (2): 239-243. doi:10.1016/j.exer. ...
Biotech will continue to drive demand for automated cell culture systems market; what other factors will create opportunities ... Automated Cell Culture Systems Market. 2020 Analysis and Review: Automated Cell Culture Systems Market by Cell Culture - Finite ... Automated Cell Culture Systems Market Outlook & Key Findings. *Automated cell culture systems market is expected to witness a ... Automated Cell Culture Systems Market: Key Players. The automated cell culture systems market players are focusing on offering ...
Cell culture technologies at that time didnt allow for good data reproducibility, because stem cells were cultured in 2D. ... Cell culture technologies at that time didnt allow for good data reproducibility, because stem cells were cultured in 2D. ... 14:10 PANEL DISCUSSION: Will 3D Cell Culture replace animal models?. * Advantages and disadvantages of 3D Cell Culture vs ... He has over 25 years experience in cell biology with specific interests in cell culture technology, neuroscience and stem cell ...
Cell Culture studiesCell Culture studies -We have previously shown SPIO particles are taken up avidly by macrophages. Our cell ... Cell culture study * 1. Cell Culture studiesCell Culture studies -We have previously shown SPIO particles are taken up avidly ... 2. Cell Culture StudiesCell Culture Studies We have Comparison of the 3 different SPIOs in the cell culture, and their effect ... Our cell culture studies showed a transient elevation in free radical of macrophages with a rapid return to the baseline. -Next ...
3D Cell Culture. 3D Cell Culture. Sophisticated in vitro 3D cell culture models have a major impact on advancing cell culture. ... Cell Culture. Cell Culture. Create a more in vivo-like cell culture to advance your discoveries right from the start. ... 2D cell culture has had an extraordinary place in cell culture history and will remain a viable cell culture model for many ... Sophisticated in vitro 3D cell culture models have a major impact on advancing cell culture. ...
... primary cell cultures potentially contain more than one type of cell. Interpretation of data arising from studies using these ... Freshney RI (1987). Cloning and selection of specific cell types. In: Culture of Animal Cells: A Manual of Basic Technique, pp ... Due to the heterogeneous nature of animal organs, primary cell cultures potentially contain more than one type of cell. ... Satellite cells form fibers of different types in cell culture. Devel Biol 143: 320-334.Google Scholar ...
In Epithelial Cell Culture Protocols, a team of well-versed experimenters and cli ... Many powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. ... Co-Culture and Crosstalk between Endothelial Cells and Vascular Smooth Muscle Cells Mediated by Intracellular Calcium ... and the interaction of epithelial cells with bacteria. Several protocols cover the culturing of epithelial cells and their use ...
... hard to detect bacteria that can infect cell cultures. Contamination can be kept to a minimum by following these steps. ... Detecting mycoplasma in cell cultures. A myriad of detection methods exist for mycoplasmas in cell culture. Some of these are ... Cell cultures are increasingly used in both research and biotechnology. While they offer valuable insight into cell physiology ... Mycoplasma commonly infect cell cultures within incubators and easily spread between cultures. Mycoplasma contamination is ...
... and they continue to strengthen our understanding of this essential aspect of cell structure and function. ... Membrane and organelle assembly has emerged as a dominant theme in cell biology of the twenty-first century. Current approaches ... Merging cultures in the study of membrane traffic. *Randy Schekman1. Nature Cell Biology volume 6, pages483-486(2004)Cite this ... Schekman, R. Merging cultures in the study of membrane traffic. Nat Cell Biol 6, 483-486 (2004) doi:10.1038/ncb0604-483 ...
For the THP-1 cells, slides were pre-treated with BD Cell-Tak Cell and Tissue Adhesive in a 24-well tissue culture plate (BD ... in conventional cell culture systems and mesenchymal stem cells inside biomimetic hydrogels that supplied a 3D cell culture ... Monocyte differentiation and cell attachment to slides. THP-1 cells (CD34±) were cultured in RPMI 1640 medium with 2 mM L- ... 3D cell culture opens new dimensions in cell-based assays. . Drug Discov. Today 14, 102-107 (2009). ...
Cell Culture Flow Systems. Lonza For info: 800-638-8174 ... The system consists of interconnected cell culture chambers and a peristaltic pump to create a continuous flow of media over ... Quasi Vivo Systems are a series of advanced, interconnected cell culture flow systems, specifically designed to improve ... including submerged cell culture, coculture, and modeling of air-liquid and liquid-liquid interfaces. Not only is the system ...
... Stem Cell Training course: Human Pluripotent Stem Cells in Culture. 2017-2018 - Course ... basic cell culture training course focusing on induced pluripotent stem cells (iPSC) and human embryonic stem cells (hESC). The ... Assessment of PSC culture. • Differentiation of human ES cells: Formation of spontanteous embryoid boides (EB) and defined EBs ... Lecture 5: The effect of reduced oxygen tension on feeder and feeder free culture of human pluripotent stem cells.. Practical ...
... buffer Cancer cell culture cell density cell growth cell lines cell numbers cell population cell suspension cell type ... culture.html?id=g5xqAAAAMAAJ&utm_source=gb-gplus-shareAnimal cell culture. ... Cell culture. Language Arts & Disciplines / Linguistics / General. Medical / Genetics. Science / Life Sciences / Cytology. ... Animal Cell Culture: A Manual of Basic Techniques. Tajamul Islam Shah. Limited preview - 2018. ...
Cell Culture & Analysis Cell Culture & Analysis * Cell Culture Dishes, Plates and Flasks ... Derived from hES Cells). Includes 1 million viable cells of Human Mesenchymal Stem Cells (derived from hES cells). ... R&D Systems™ Cultrex™ Stem Cell Qualified Human Fibronectin PathClear™. R&D Systems™ Cultrex™ Stem Cell Qualified Human ... R&D Systems™ Cultrex™ Stem Cell Qualified Human Vitronectin PathClear™. R&D Systems™ Cultrex™ Stem Cell Qualified Human ...
The Genomics building has two fully equipped rooms dedicated to cell culture, which are part of the second and third floor ... ... Dedicated culture rooms. GE S110.01, SP 550.04. SP 430.01 (Whiteway).. Incubation Shakers. GE S110.05, GE S110.09, GE 230.00, ... Each culture room is equipped with two biological safety cabinets, a stack of three large incubation shakers, a refrigerated ...
... duplicated and extended results of the animal experiments in a neural cell culture assay. In culture assays it was possible to ... "Cell Cultures Can Sort Out CJD and Scrapie Infectious Agents." Medical News Today. MediLexicon, Intl., 28 Oct. 2005. Web.. 22 ... Nordqvist, C. (2005, October 28). "Cell Cultures Can Sort Out CJD and Scrapie Infectious Agents." Medical News Today. Retrieved ... Cells infected with a weak strain of CJD were also protected from infection by two strains of sheep scrapie agent. "We ...
... in which the culture medium comprises an elevated concentration of a thymidine family member, in which the growth or viability ... We describe a method of growing an animal cell in a culture medium, ... Specific examples of cells include CHO cells, HeLa cells, HEK cells, HEK 293 cells, COS1 cells, embryonic stem cells, African ... Animal Cell The improved cell culture medium is suitable for the culture of any cell or colony or cell line preferably of ...
This webinar reviews some of the systems available,best practices when imaging 3D cell culture mode... ... There are many important considerations for how best to image a 3D cell culture model. Moreover, there are numerous high ... This provides an efficient, scalable cell culture vessel for adherent cell culture processes common to gene and cell therapy ... 3D Cell Cultures and Tissue Clearing. There are many important considerations for how best to image a 3D cell culture model. ...
Cell Culture & Analysis Cell Culture & Analysis * Cell Culture Dishes, Plates and Flasks ... Features a superior cell culture surface with demonstrated performance that elevates stem cell and cell culture research. ... Thermo Scientific™ BioLite Cell Culture Treated Dishes Perform general cell culture procedures more economically with Thermo ... Corning™ TC-Treated Culture Dishes Tissue-culture (TC) treated for optimum attachment and growth of anchorage-dependent cells. ...
... , Date: 3 Sep 93 05:03:13 GMT , I know that plant cell and tissue culture has been around for long time. , , ... How is it that high value products such as vanilla, taxol, quinine etc , are not widely produced by plant cell culture ? ... Plant cell culture. SPLUHAR at CROP.UOGUELPH.CA SPLUHAR at CROP.UOGUELPH.CA Sun Sep 5 23:19:15 EST 1993 *Previous message: ... An example of such unstable types of cells is Alfalfa suspension cells, which frequently loose chromosomes and double others, ...
Cells are cultured in controlled conditions including temperature, gas exchange, pH, and culture media (energy and nutrients). ... This webinar will introduce basic concepts of cell cultur... ... Cell culture is a fundamental technique for a variety of ... Cell Culture Fundamentals. Cell culture is a fundamental technique for a variety of applications. Cells are cultured in ... Introduction to cell culture. •Critical factors for successful cell culture. •Cell culture tips and techniques. Presenter Bio: ...
For example, human breast epithelial cells proliferate abnormally (like tumor cells) when cultured as a two-dimensional ... more efficiently to blood-forming stem cells when cultured in three-dimensional scaffolds compared to cells cultured in two ... Cell-initiated proteolysis of chemical cross-links in a gel allows cell migration by mimicking proteolytic processes that occur ... At the start of the experiment, the cell-laden gel contains rounded cells with few interactions (left). Over the course of 2 ...
2D cell cultures lack the structure, function, dimensionality, cellular diversity, and cell-cell interactions that make living ... Spheroids, Organoids Replacing Standard Cultures for Cell-Based Assays. Corning Life Sciences maintains that 3D cell cultures ... Three-dimensional (3D) cell culture overcomes many shortcomings of two-dimensional (2D) culture, mainly through the ... Like any emerging technology, 3D cell culture has advanced in several directions, most notably as 3D suspended cultures, known ...
Spatially patterned vascular cell co-cultures for combined electrical-optical monitoring of cell-cell communication. A ... vascular cell isolation and culture, and live-cell imaging.. Further Information. This studentship will commence on 1st October ... Previous experience in either microengineering systems or cell culture methods would be advantageous. Project Details. ... It will focus on the development of a new approach to dynamically monitoring vascular cell-cell communication by creating a ...
Mouse Müller monolayer cells cultured for 10 days after one passage (A) and the cultured cells are stained with Müller cell- ... Mouse; Müller glial cells; Primary cell culture; Retinal cell isolation; Stem cells; Worthington Papain Kit ... Mouse Müller Cell Isolation and Culture.. Liu X1,2, Tang L2, Liu Y1. ... Müller cells are the major supportive and protective glial cells across the retina. Unlike in fish, they have lost the capacity ...
Cell Culture News and Research. RSS Cell culture is the process of isolating cells from an organism and growing them in a ... INTEGRA offers new 24-channel pipetting heads for VIAFLO 96/384 to make cell culture easy INTEGRA has responded to customers ... Lab-Scale Clarification of Mammalian Cell Cultures Expressing Recombinant Antibodies Tina Stoschek and Marcus Gerlach discuss ... improves hepatocyte quality and enables cells from a wider quality spectrum to be used in standard and advanced cell culture. ...
3D cell culture is expanding in multiple directions, exploring new possibilities in basic research, drug discovery, and ... Trezise: Improvements in the 3D cell culture toolkit include cell-imaging modalities, cell biomatrices, and stem-cell ... Only when 3D cell cultures can be created, manipulated, and analyzed as readily as can 2D cell cultures, will the true promise ... cells, genetically engineered cells, induced pluripotent stem cell-derived cells, primary human cells, and patient-derived ...
Europes leading Cell Culture and Downstream Processing conference for upstream and downstream manufacture ... Cell Culture & Downstream World Congress 2017. 21 - 22 February. Sofitel Munich Bayerpost, Munich ...
... particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A ... Cell Sorting, 347. 18. Cell Line Characterization, 359. 19. Differentiation, 383. 20. Three ]Dimensional Culture 401. 21. Scale ... Culture of Specific Cell Types, 531. 25. Supplementary Material* 26. Stem Cells, 571. 26. Supplementary Material* 27. Training ... 2. Biology of Cultured Cells, 19. 3. Laboratory Design and Layout, 37. 4. Equipment and Materials, 51. 5. Aseptic Technique, 73 ...
... and CMOS to provide new content on process development for cell-culture specific scientists. ... Cell Culture World Congress USA connects pharma, biotech, ... Cell Culture World Congress USA 2017. 23 - 24 May. Hilton San ... Cell Culture World Congress USA is focusing on using analysis of metabolic dynamics as a systems biology approach to CHO cell ... enhancement, bridging the gap between upstream and downstream manufacturing, and how we can enhance (and replace!) CHO cells in ...
Cell culture is set to see huge increases in growth including the recent advances in techniques such as 3D cell culture. The ... Day twos speaking faculty will focus on cell culture process development and cell line development including the use of high ... 11:00 Discriminating drug effects in multi-cell type 3D cell culture models. ... Day one will focus on the recent advances in 3D cell culture. Case studies will demonstrate how this method is providing highly ...
  • Many powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. (
  • The emphasis is on the analysis and assessment of epithelial cells, for example, by looking at apoptosis and integrins or by measuring membrane capacitance and confluence. (
  • Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. (
  • Several protocols cover the culturing of epithelial cells and their use in treating patients with burns and other skin disorders. (
  • Wide-ranging and highly practical, Epithelial Cell Culture Protocols offers both novices and expert investigators alike a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms in general, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells. (
  • However, certain species have been discovered in granulocytes and monocytes, after phagocytosis, but also inside epithelial cells through non-phagocytotic pathways. (
  • For example, human breast epithelial cells proliferate abnormally (like tumor cells) when cultured as a two-dimensional monolayer, but display normal cell growth behavior and form structures typical of breast tissue when cultured in three-dimensional membranes that resemble their native environment ( 1 ). (
  • This serum-free system is designed to support airway epithelial cells derived from normal human lung. (
  • To support the proliferation and plating efficiency of various types of airway epithelial cells, Airway Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit. (
  • When using this complete media system, the growth of airway epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates. (
  • For airway epithelial cells derived from bronchial/tracheal tissue (e.g. (
  • Primary Bronchial/Tracheal Epithelial Cells, Normal, Human, ATCC PCS-300-010), supplement Airway Epithelial Cell Basal Medium with the Bronchial Epithelial Cell Growth Kit (ATCC PCS-300-040). (
  • For small airway epithelial cells (e.g. (
  • This system is designed to support epithelial cells (e.g., renal proximal tubule epithelial cells) derived from normal human kidney. (
  • To support the proliferation and plating efficiency of renal epithelial cells (e.g. (
  • Primary Renal Mixed Epithelial Cells, Normal, Human, ATCC PCS-400-012), Renal Cell Basal Medium must be supplemented with the Renal Epithelial Cell Growth Kit (ATCC PCS-400-040). (
  • Using Renal Epithelial Cell Basal Medium supplemented with the Renal Epithelial Cell Growth Kit, the growth of renal epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates. (
  • In this study, Aryan et al used hydrogen peroxide, a highly reactive compound, to induce oxidative stress in human retinal pigment epithelial cells, a type of ocular cell which provides nourishment for the human retina. (
  • This group has recently shown that epithelial cells extracted from the developing teeth of 6-month-old pigs continue to proliferate when they are cultured on top of a special feeder layer of cells (the feeder-layer cells are known as the 3T3-J2 cell line). (
  • This crucial step boosts the number of dental epithelial cells available for enamel production. (
  • In the study being reported today, the researchers seeded the cultured dental epithelial cells onto collagen sponge scaffolds, along with cells from the middle of the tooth (dental mesenchymal cells). (
  • The key finding of this study was that even after the multiple divisions that occurred during propagation of the cells in culture, the dental epithelial cells retained the ability to produce enamel, as long as they were later provided with an appropriate environment. (
  • The idea for the culturing technique originates from 1975, when Dr. J.G. Rheinwald and Dr. H. Green of Harvard Medical School reported the use of feeder layers for culturing epithelial cells from the skin (the 3T3-J2 cells used in the current study were gifted by Dr. Green). (
  • Now that dental epithelial cells can be propagated in culture, the next step will be to achieve the same success with their partners in tooth formation, the dental mesenchymal cells. (
  • Based on previous publications on storage of cultured epithelial cells [ 20 - 23 ], we hypothesize that differences in storage temperature between 4°C and 37°C affect the viability, morphology, and phenotype of cultured RPE cells. (
  • Iwould think there would be plenty of references in is Journal of Tissue CulTure Methods. (
  • In: Tissue Culture Techniques: An introduction pp 143-151. (
  • Tissue-culture (TC) treated for optimum attachment and growth of anchorage-dependent cells. (
  • Corning Falcon™ Standard Tissue Culture Dishes works with most attachment-dependent cell lines. (
  • Corning™ Falcon™ Easy-Grip Tissue Culture Dishes are uniquely designed to simplify lifting one dish or a small stack of dishes. (
  • Corning™ Primaria™ Tissue Culture Dishes feature flat, optically clear polystyrene surfaces for distortion-free microscopic visualization of cells. (
  • Naturally based" hydrogels such as Matrigel (made of native extracellular matrix proteins) and collagen (the major extracellular matrix protein) have been explored extensively for applications in three-dimensional tissue culture and regenerative medicine. (
  • Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies. (
  • While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. (
  • Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the previous editions' tradition of exceptional quality and thorough coverage in tissue culture protocols for today's neuroscience in order to inspire researchers in the field to further explore this imperative area of study. (
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. (
  • Airway Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC ® Primary Cell Solutions™ system. (
  • Without a clearing treatment, a 3-D tissue culture, top, does not allow researchers to see deep inside. (
  • Maintaining the size of the tissue culture allows the researchers to determine its physical dimensions of growth. (
  • Her elegantly written and well-documented narrative demonstrates how, within 20th-century biological laboratories, a whole range of vessels and containers were used in order to store, breed, study, and manipulate cells and tissue outside the body of organic [End Page 312] individuals: from hanging-drop preparations in the 1910s to tissue culture flasks in the 1930s and powerful freezers in the 1950s and 1960s. (
  • As Landecker shows, however, the rather unexpected adoption of Harrison's technique by the French experimental surgeon Alexis Carrel (from the Rockefeller Institute for Medical Research) crucially contributed to establishing the method of tissue culture in medical and biological contexts. (
  • The present volume discusses the uptake, synthesis, and degradation of biologically important compounds, particularly the major components usually present in tissue culture medium. (
  • The book begins by tracing the history of the development of tissue culture. (
  • British biologist who developed ways to grow cells outside the body ("tissue culture") in order to more closely study the cells and the effects of hormones, vitamins, and other chemicals. (
  • Cells and Tissues in Culture: Methods, Biology, and Physiology, Volume 3 focuses on the applications of the methods of tissue culture to various fields of investigation, including virology, immunology, and preventive medicine. (
  • The selection first offers information on molecular organization of cells and tissues in culture and tissue culture in radiobiology. (
  • Topics include cellular organization at the molecular level, fibrogenesis in tissue culture, effect of radiation on the growth of isolated cells, and irradiation of the selected parts of the cell. (
  • The book elaborates on antibody production in tissue culture and tissue culture in pharmacology. (
  • Discussions focus on early attempts at in vitro studies, tissue culture in the study of pharmacologically active agents, and methods of assessment of drug activity. (
  • Corning™ Stripette™ Individually Wrapped Plastic or Paper/Plastic Disposable Polystyrene Serological Pipets are ideal for sterile tissue culture applications, especially while wearing latex gloves, as the wrap reduces static cling. (
  • Corning™ Falcon™ Tissue Culture Treated Flasks features vacuum-gas plasma tissue culture treatment to ensure consistent growth surface. (
  • More than a container for plant cell or tissue culture. (
  • Sterilized Cover for PLANTCON ™ Plant Tissue Culture Container. (
  • provides assistance in acquiring those skills that are critical for plant tissue culture and plant biotechnology research. (
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). (
  • In 1885, Wilhelm Roux removed a portion of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the principle of tissue culture. (
  • Ross Granville Harrison, working at Johns Hopkins Medical School and then at Yale University, published results of his experiments from 1907 to 1910, establishing the methodology of tissue culture. (
  • If additional in-house or send-out testing on cultured cells (fibroblasts) is desired, please clearly indicate the desired testing on the requisition form and call Cytogenetics lab at (614) 722-5321 and speak to a laboratory genetic counselor to coordinate testing. (
  • In the 1960s, biologists found that normal human fibroblasts, cells derived from connective tissue , had a predictable limit in their ability to proliferate in culture. (
  • Induction of pluripotent stem cells from adult human fibroblasts by defined factors," Cell , vol. 131, no. 5, pp. 861-872, 2007. (
  • Growing incidences of chronic diseases have resulted in upswing in research related to cell biology and cytology. (
  • He has over 25 years experience in cell biology with specific interests in cell culture technology, neuroscience and stem cell research. (
  • Scientific advances in cancer cell biology are changing the paradigm in oncology research. (
  • Membrane and organelle assembly has emerged as a dominant theme in cell biology of the twenty-first century. (
  • Current preclinical GBM models are limited by the lack of a "normal" human microenvironment and the inability of many tumor cell lines to accurately reproduce GBM biology. (
  • The project will provide the opportunity to develop expertise in a wide range of both engineering and cell biology techniques, including microfabrication and lab-on-a-chip techniques, electrochemical sensors, vascular cell isolation and culture, and live-cell imaging. (
  • This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians. (
  • We propose to apply a systems biology approach to achieve a computational representation of tissue-level physiological responses by integrating empirical data derived from organotypic culture systems with computational models of intracellular pathways to better predict human responses. (
  • Vertebrate, insect, and plant cell lines are important tools for research in many disciplines, including human health, evolutionary and developmental biology, agriculture and toxicology. (
  • Culturing particular types of cells in isolation is a basic technique for measuring how they respond to various stimuli, testing new drugs, and similar cell biology tasks. (
  • The general process of cell culture has been used extensively since the early 1900s for research on tissue growth and development, virus biology, properties of cancer cells, studies relating to aging, genetics, and gene therapy. (
  • Long-term proliferation in culture and germline transmission of mouse male germline stem cells," Biology of Reproduction , vol. 69, no. 2, pp. 612-616, 2003. (
  • 3D cell culture enables cells to grow and interact with their surroundings in all three dimensions, better mimicking the in-vivo conditions in which the cells are naturally present. (
  • Create a more in vivo-like cell culture to advance your discoveries right from the start. (
  • Yet with the rise of promising new opportunities within such fields of study as cancer research and stem cell differentiation, using 2D models to obtain useful information about in vivo biological processes has created challenges. (
  • As research demands have become increasingly complex, we have been there every step of the way, creating 3D cell culture solutions that allow you to produce optimal environments for growing cells that exhibit in-vivo like behaviors and functionality. (
  • Labeling characteristics of satellite cells in vivo . (
  • Cell culture systems that better recapitulate the physiological conditions and environment a cell experiences in vivo can improve our understanding of cellular behaviour. (
  • Quasi Vivo Systems are a series of advanced, interconnected cell culture flow systems, specifically designed to improve cellular phenotypes and human in vivo relevance of in vitro cell culture models. (
  • She has worked with xenograft models and many cancer cell lines, and she has expertise in in vitro cell based functional assays and in vivo experiments. (
  • Sartorius Stedim Biotech, a leading international supplier for the biopharmaceutical industry today announced the launch of the BIOSTAT RM TX single-use bioreactor, a new wave mixed system developed specifically for closed, automated expansion of consistent quality cell products such as ex vivo cellular immunotherapies. (
  • It is becoming increasingly clear that these models mimic cells in vivo at a greater capacity than traditional cell culture. (
  • It uses a novel method to create high-density collagen scaffolds with cells embedded, enabling improved cellular interactions that more closely resemble an in vivo environment. (
  • Primary cells are considered by many researchers to be more physiologically similar to in vivo cells. (
  • The pluripotency of ES and EG cells can be demonstrated in vitro and in vivo . (
  • Perhaps the most definitive in vivo test of developmental potential would be a demonstrated contribution to all cell lineages in a chimeric animal, but this test is not practical or possible for all species and cannot be done with human cells. (
  • Cells can be isolated from tissues for ex vivo culture in several ways. (
  • In Jul 2020, Incucyte Live-Cell Analysis System and Incucyte 3D Multi-Tumor Spheroid Assays were used for evaluating 3D spheroids' development with either non-invasive reagents or label-free in real time. (
  • In colony assays, samples from cell cultures are inoculated into mycoplasma broth and onto agar 4-7 days after inoculation. (
  • In culture assays it was possible to test human and sheep agent strain combinations that could not be discriminated in animals. (
  • In addition to spheroids, collagen-based scaffolds that encourage cell aggregation into tumoroids have been used for immunotherapy applications such as natural killer cell cytotoxicity assays. (
  • Physiological relevance is a key parameter to improve predictivity of cell-based assays in drug discovery and toxicity testing for compound. (
  • The µ-Dish 35-mm Quad from Ibidi GmbH is a four-compartment cell culture dish that can be used for simultaneous assays, guaranteeing high optical quality. (
  • This week (and a half) in Portland Community College's Bioscience Technology Program we continued work with assays, and had guest speakers in Current Topics and Cell Culture. (
  • The turkey myogenic satellite cell: Optimization of in vitro proliferation and differentiation. (
  • Further, we demonstrate for the first time a volumetric quantification of endogenous biomolecules using 3D Raman imaging datasets that allows us to spatially monitor complex biological processes like differentiation within a 3D cell culture system. (
  • Oxygen-sensing transcription systems, such as hypoxia-inducible factors, play an important role in regulating the differentiation of stem cells ( 3 ), as does the distribution of diffusing growth factors. (
  • However, the complexity, variability, and ill-defined nature of these interactions make it difficult to understand the proliferation, differentiation, and migration of cells embedded within these natural gels. (
  • In basic research, 3D cell culture is being used to establish models of disease and unravel the mysteries of differentiation. (
  • Here we evaluated the transformation of electrostatically stabilized citrate nanoparticles (C-AgNPs) and sterically stabilized polyvinylpyrrolidone nanoparticles (PVP-AgNPs) in a low-serum (~ 0.2 mg/mL bovine serum albumin) medium, while measuring the response of neural progenitor cells undergoing differentiation. (
  • and growth, differentiation and organogenesis in plant tissue and organ cultures. (
  • With the proper combinations of growth and differentiation factors, mouse ES ( 22 , 23 ) and EG (S.W., unpublished results) cultures can generate cells of the hematopoietic lineage and cardiomyocytes ( 24 , 25 ). (
  • A couple of weeks ago, I was giving a plate of undifferentiated stem cells to practice passaging and removing unwanted differentiation. (
  • This is a hands-on, basic cell culture training course focusing on induced pluripotent stem cells (iPSC) and human embryonic stem cells (hESC). (
  • R&D Systems™ Cultrex™ Stem Cell Qualified Human Fibronectin PathClear™ provides a functionally defined and effective feeder-free surface for the attachment and maintenance of embryonic stem cells in a pluripotent state. (
  • To address these limitations, our guest presenter and her team have established a unique model system whereby they can retro-engineer patient-specific GBMs using patient-derived glioma stem cells (GSCs) and human embryonic stem cell (hESC)-derived cerebral organoids. (
  • Embryonic stem cells differentiate more efficiently to blood-forming stem cells when cultured in three-dimensional scaffolds compared to cells cultured in two dimensions ( 2 ). (
  • Science Daily reports that for the first time, human embryonic stem cells have been cultured under chemically controlled conditions without the use of animal substances, which is essential for future clinical uses. (
  • Now, for the first time, we can produce large quantities of human embryonic stem cells in an environment that is completely chemically defined," says professor Karl Tryggvason, who led the study at Sweden's Karolinska Institutet. (
  • IPsC are when they take cells from your own body and make them revert back to a similar state to embryonic stem cells, to where they can then be turned into any cell type you want (the advantage there being they're your cells so you wouldn't get tissue rejection like you would with embryonic stem cells. (
  • Via homologous recombination in embryonic stem cells (ES cells), it is possible to generate a specific genetic modification at a specific location in the genome. (
  • Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice. (
  • 2. The method of claim 1 , wherein the human pluripotent stem cells are embryonic stem cells. (
  • The invention relates to stem cells (SC) in particularly to methods and systems for handling human embryonic stem cells (hESC). (
  • Embryonic stem cell lines derived from human blastocysts. (
  • Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. (
  • Feeder-free growth of undifferentiated human embryonic stem cells. (
  • At the same time, he was propagating und publicizing a new image of life that could be understood as a literal embodiment of durée in the sense of philosopher Henri Bergson: in 1912, Carrel placed tissue from an embryonic chicken heart in a specifically designed flask and maintained this living culture for over 20 years. (
  • Gibco™ Neurobasal™ Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used with Gibco™ B-27™ Supplement. (
  • Over a period of 7-21 days, PGCs gave rise to large multicellular colonies resembling those of mouse pluripotent stem cells termed embryonic stem and embryonic germ (EG) cells. (
  • Throughout the culture period most cells within the colonies continued to be alkaline phosphatase-positive and tested positive against a panel of five immunological markers (SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81) that have been used routinely to characterize embryonic stem and EG cells. (
  • Immunohistochemical analysis of embryoid bodies collected from these cultures revealed a wide variety of differentiated cell types, including derivatives of all three embryonic germ layers. (
  • Pluripotent stem cells have been derived from two embryonic sources. (
  • Embryonic stem (ES) cells are derived from the inner cell mass of preimplantation embryos ( 1 , 2 ), and embryonic germ (EG) cells are derived from primordial germ cells (PGCs) ( 3 , 4 ). (
  • Other important characteristics include growth as multicellular colonies, normal and stable karyotypes, the ability to be continuously passaged, and the capability to differentiate into cells derived from all three embryonic germ layers. (
  • This property alone may not be a definitive test of stem cell pluripotency, as it has been demonstrated that rat and mouse visceral (yolk sac) endoderm are capable of forming highly differentiated teratomas containing cells of all three embryonic germ layers ( 29 , 30 ). (
  • Over the past few years, 3D Cell Culture has gained momentum within the pharmaceutical industry due to the benefits that this model offers over conventional cell culture methods. (
  • Methods of cell separation. (
  • McFarland, D.C. Methods Cell Sci (2000) 22: 63. (
  • In Epithelial Cell Culture Protocols, a team of well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. (
  • A myriad of detection methods exist for mycoplasmas in cell culture. (
  • Before these approaches, polymerase chain reaction (PCR), DNA staining and microbiological cultures were the commonly used methods. (
  • The specificity of PCR is appealing, as knowing the type of mycoplasma the cell culture is infected with can be beneficial for future prevention methods. (
  • Previous experience in either microengineering systems or cell culture methods would be advantageous. (
  • Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death. (
  • Research activities are focused on the development of methods for cryopreservation and characterisation of cell cultures for the monitoring of genetic and epigenetic stability. (
  • Provided are systems and methods for providing human cell cultures. (
  • Further provided are cultures of feeder cells for use in stem cell technology, as well as cultures, culture systems and methods for maintenance and propagating of stem cells in an undifferentiated state as well as for the development. (
  • If you are adopting the next-generation technologies to improve your daily lives such as smart phones, then why stick to the old cell culture methods and compromise results in the lab? (
  • Primary cell cultures come from the outgrowth of migrating cells from a piece of tissue or from tissue that is disaggregated by enzymatic, chemical, or mechanical methods. (
  • After the Second World War, methods of culturing tissue were further improved and provided the basis for a genuine mass (re)production of cells. (
  • Accordingly, most culture methods require sterile conditions. (
  • GBI Research, the leading business intelligence provider, has released its latest research report, Manufacturing of Vaccines - Cell Culture Technology Gradually Replacing Egg-Based Manufacturing, which provides insights into the production methods of vaccines and into vaccine production capacity. (
  • The establishment of future retinal pigment epithelium (RPE) replacement therapy is partly dependent on the availability of tissue-engineered RPE cells, which may be enhanced by the development of suitable storage methods for RPE. (
  • With the advancement of RPE cell replacement therapy, and with 20-25 million known sufferers from AMD worldwide [ 17 ], a great need for improved storage methods for cultured RPE is likely to emerge. (
  • The published results are in stark contrast to current Petri culturing or PCR Protocol (DNA sequencing) methods. (
  • Other topic areas which will be featured at Cell Culture 2012 are the challenges involved with preventing cell line cross contamination and the methods used to mitigate these risks, as well as novel methods in the use of 3D cell cultures. (
  • This guide contains general technical information for working with animal cells in culture, including media, subculturing, cryopreservation and contamination. (
  • This enhances the ability for scientists to study basic biological mechanisms such as cell number monitoring, cell viability, proliferation and morphology. (
  • Successful cloning is dependent on optimization of attachment substrata, basal media composition, serum source, and growth factor/hormone additions to support proliferation of the cell type at clonal (low) density. (
  • Proliferation of the turkey myogenic satellite cell in a serum-free medium. (
  • This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. (
  • This attachment is essential for proliferation - many adherent cell cultures will cease proliferating once they become confluent (i.e., when they completely cover the surface of cell culture vessel), and some will die if they are left in this confluent state for too long. (
  • Interestingly, cells engineered to express more telomerase retained telomeres and the ability for extended proliferation. (
  • Oxidative stress resulted in a profound influence on advancing the senescence (functional deterioration) of these cells and inhibiting their proliferation. (
  • Long-term proliferation and characterization of human spermatogonial stem cells obtained from obstructive and non-obstructive azoospermia under exogenous feeder-free culture conditions," Cell Proliferation , vol. 43, no. 4, pp. 405-417, 2010. (
  • Incucyte S3 live-cell analysis system was devised lately for defining spontaneous neuronal activity as well as connectivity from hiPSC (human-induced pluripotent stem cells)-derived neurons after transducing GECI (genetically encoded calcium indicator). (
  • We apply qVRI to a selection of biological systems: human pluripotent stem cells with their cardiac derivatives, monocytes and monocyte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside biomimetic hydrogels that supplied a 3D cell culture environment. (
  • We use qVRI to image: human pluripotent stem cells and monocytes/macrophages in conventional culture systems, as well as mesenchymal stem cells in 3D biomaterials. (
  • Lecture 5: The effect of reduced oxygen tension on feeder and feeder free culture of human pluripotent stem cells. (
  • Ludmila Ruban, Cell Therapy Research Facilitator and Training Coordinator and author of 'Human pluripotent stem cells in culture', recently published by Springer. (
  • Organoids can also be produced from induced pluripotent stem cells. (
  • For example, renal organoids derived from pluripotent stem cells have been successfully transplanted under the renal capsules of adult mice. (
  • I would guess that the next step, maybe one they're already working on, is to show that induced pluripotent stem cells can be cultured on this same protein. (
  • it's hard to completely differentiate a population of pluripotent cells into one cell type you're trying to get. (
  • maintaining the human pluripotent stem cells in an undifferentiated state for at least 10 weeks. (
  • 3. The method of claim 1 , for propagating the human pluripotent stem cells in an undifferentiated state. (
  • Human pluripotent stem cells would be invaluable for in vitro studies of aspects of human embryogenesis. (
  • With the goal of establishing pluripotent stem cell lines, gonadal ridges and mesenteries containing primordial germ cells (PGCs, 5-9 weeks postfertilization) were cultured on mouse STO fibroblast feeder layers in the presence of human recombinant leukemia inhibitory factor, human recombinant basic fibroblast growth factor, and forskolin. (
  • Based on their origin and demonstrated properties, these human PGC-derived cultures meet the criteria for pluripotent stem cells and most closely resemble EG cells. (
  • Both ES and EG cells are pluripotent and demonstrate germ-line transmission in experimentally produced chimeras ( 5 , 6 ). (
  • These properties are characteristic of, but not specific for, pluripotent stem cells. (
  • Pluripotent stem cell lines that share most of these characteristics also have been reported for chicken ( 10 ), mink ( 11 ), hamster ( 12 ), pig ( 13 , 14 ), rhesus monkey ( 15 ), and common marmoset ( 16 ). (
  • These aggregates range from a cluster of pluripotent stem cells enclosed by a layer of endoderm to complex structures closely resembling an embryo during early development. (
  • EBs from mouse pluripotent stem cells grown on feeder layers or in suspension may contain a variety of cell types. (
  • Derivation of pluripotent stem cells from cultured human primordial germ cells," Proceedings of the National Academy of Sciences of the United States of America , vol. 95, no. 23, pp. 13726-13731, 1998. (
  • Isolation and characterization of pluripotent human spermatogonial stem cell-derived cells," Stem Cells , vol. 27, no. 1, pp. 138-149, 2009. (
  • ECACC, which was established in 1985, consists of a team with specialist knowledge which supply authenticated cell lines, induced Pluripotent stem cells (iPSCs) and nucleic acids to provide stock for the research community. (
  • Spheroids are simple clusters of broad-ranging cells collected and cultured from various tissues, for example, from an organ or, by way of a biopsy, from a diseased tissue or tumor. (
  • 1. The technique or process of growing bacterial or fungal cells or cells derived from tissues of living organisms in a culture medium. (
  • Most cells derived from tissues are anchorage-dependent. (
  • A team at Brown University made the finding while studying how neural tissues grow from stem cells. (
  • ClearT2, one of three new chemical treatments evaluated in recent tests, makes tissues transparent, bottom, so that researchers can study cell interactions beneath the surface. (
  • As I was imaging these tissues I was only able to get the outer layer or two of cells, and that wasn't a very good representation of what was going on inside of the sphere," said Molly Boutin, a graduate student in biomedical engineering. (
  • The publication then considers the effects of invading organisms on cells and tissues in culture and cell, tissue, and organ cultures in virus research. (
  • The selection is a vital source of data for readers interested in the culture of cells and tissues. (
  • The cell-scaffold approach is based on tissue-engineering technology developed at the Forsyth Institute (MA) and was applied by one of the Tokyo researchers to produce enamel-like tissues in 2002. (
  • Rapidly isolates primary cells to consistently obtain a uniform single-cell suspension from tissues. (
  • Cells can be isolated from solid tissues by digesting the extracellular matrix using enzymes such as collagenase, trypsin, or pronase, before agitating the tissue to release the cells into suspension. (
  • While they offer valuable insight into cell physiology and are a good alternative to animal models, they have, in recent decades, been proven to be vulnerable to contamination. (
  • If tissue from products of conception (POC) is submitted and molecular genetic testing is requested on cultured cells, then submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study. (
  • cell cultivation via incubators, incubation and benchtop shakers and cell harvest via homogenisers and centrifuges with varoius volumes as well as PCR-based tests for mycoplasma contamination control. (
  • If cultured amniocytes will be used to perform fetal molecular genetic testing, submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study. (
  • The limitations of cell culture include the finite doubling potential of most normal cells, the possibilities for unexpected infection with viruses or microorganisms, or even cross-contamination with other cell types. (
  • One complication of these blood-derived ingredients is the potential for contamination of the culture with viruses or prions, particularly in medical biotechnology applications. (
  • This eliminates the worry of cross-species contamination when using FBS with human cells. (
  • In the development of the portfolio we put a special focus on your needs for excellent cell performance and reliability as well as for advanced protection against contamination. (
  • If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. (
  • Matrigel, a gelatinous protein mixture derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells, contains several proteins, carbohydrates, and growth factors that are also present in tumor microenvironments. (
  • Tumor-derived spheroids reproduce both the specific tumor cell types-with all their abnormalities-and the physical-mechanical environment that makes tumors so difficult to treat-a factor totally absent when assaying cancer cells plated in 2D. (
  • Scientists have used tumor spheroids to study the cytotoxic effects of chimeric antigen receptor (CAR) T cells-such as the KILR ® Cytotoxicity assay developed by DiscoverX. (
  • When CAR T cells are grown in KILR-transduced tumor spheroids, scientists have the ability to form, culture, and assay in the same spheroid microplate. (
  • Network with industry experts, gain insight into how the pharmaceutical industry is utilizing 3D Cell Culture technologies to enhance research and development, discuss the potential for novel in-vitro cell culture models to replace animal models, and discover novel 3D cell culture systems, organ-on-chip, 3D imaging of organoids and other technologies in development. (
  • Like any emerging technology, 3D cell culture has advanced in several directions, most notably as 3D suspended cultures, known as spheroids and organoids. (
  • Spheroids and organoids both contain multiple cells, typically suspended within a droplet or microwell. (
  • As their name implies, organoids are complex clusters of organ-specific cells designed to mimic the original tissue such as the skin, stomach, liver, or bladder. (
  • Unlike spheroids, which form from tissue samples containing any number and type of mature cells, organoids arise from tissue-specific stem cells, or progenitor cells, harvested from various organs, such as the brain or liver. (
  • Patient-derived pancreatic cancer organoids cultured in Corning® Matrigel® matrix for organoid culture (2x objective). (
  • Although spheroids lack organoids' engineered complexity, their 3D structure puts them miles ahead of conventional 2D cell culture models, particularly for drug screening. (
  • Autologous tissue for transplantation may also come from organoids produced via 3D cell culture. (
  • She has extensive experience in a variety of cell culture techniques including isolation and culture of primary patient cells. (
  • Isolation and Culture of Blastocyst-Derived Stem Cell Lines. (
  • Suspension cells can survive and proliferate without being attached to a substratum. (
  • Primary cultures are formed from cells that survive the disaggregation process, attach to the cell culture vessel (or survive in suspension), and proliferate. (
  • These cultures will proliferate for a limited number of cell divisions, after which they will senesce. (
  • The cells will proliferate for an extended time, but usually the culture will eventually cease dividing, similar to senescent primary cells. (
  • An established or immortalized cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene. (
  • Skeletal muscle satellite cell diversity: Satellite cells form fibers of different types in cell culture. (
  • Satellite cells isolated from sheep skeletal muscle are not homogeneous. (
  • Page 617 - Jessell TM, Siegel RE, and Fischbach GD (1979) Induction of acetylcholine receptors on cultured skeletal muscle by a factor extracted from brain and spinal cord. (
  • In addition, mouse ES cells have been used to generate in vitro cultures of neurons ( 26 ), skeletal muscle ( 27 ), and vascular endothelial cells ( 28 ). (
  • In May 2020, HORIBA Medical entered into collaboration with CellaVision regarding automated digital cell morphology solution. (
  • Conventional 2D culture conditions are highly limited as native tissue morphology and cell composition is not properly reflected. (
  • Another difficulty with some cultured cells is their tendency to change their morphology , functions, or the range of genes they express. (
  • This study investigates the effect of different storage temperatures on the viability, morphology, and phenotype of cultured RPE. (
  • Out of nine temperatures tested between 4°C and 37°C, storage at 12°C, 16°C, and 20°C was optimal for maintenance of RPE cell viability, morphology, and phenotype. (
  • Once one culture in a laboratory has been contaminated, the mycoplasma can spread to the other cultures. (
  • It will also provide managers with insights into the intricacies of cell culture and the necessary laboratory infrastructure requirements. (
  • The Genomics building has two fully equipped rooms dedicated to cell culture, which are part of the second and third floor laboratory areas. (
  • Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines. (
  • Animal cell culture became a common laboratory technique in the mid-1900s, but the concept of maintaining live cell lines separated from their original tissue source was discovered in the 19th century. (
  • Cell culture disposables for cell cultivation and cell monitoring in laboratory scale. (
  • With her book Culturing Life , Hannah Landecker contributes an important chapter to the recent history of these laboratory fractals. (
  • Cell culture describes the laboratory growth of cells derived from plants or animals. (
  • The ability to culture cells allowed the laboratory growth of polio virus to produce vaccines that nearly eliminated polio as a disease. (
  • Corning™ Falcon™ Test Tube with Cell Strainer Snap Cap offer a convenient solution to prepare laboratory samples. (
  • In a search for alternative sources of meat and in response to concerns over meat consumption, animal welfare and greenhouse gas emissions, scientists have developed the ability to grow meat from animal cells in a laboratory. (
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (
  • The role of patterned electrical impulses has been investigated in the literature using co-cultures of neurons and myelinating cells. (
  • The co-culturing method, however, prevents the uncoupling of the direct effect of patterned electrical impulses on myelinating cells from the indirect effect mediated by neurons. (
  • Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. (
  • Cells grown in vitro from neoplastic tissue. (
  • Once provided with an appropriate extracellular matrix and treated with biochemical stimuli (specific for the eventual target cells and the tissue of interest), these progenitor cells expand, differentiate, and self-assemble into microscopic cultures that recapitulate one or more critical aspects of the original tissue. (
  • Includes 1 million viable cells of Human Mesenchymal Stem Cells (derived from hES cells). (
  • provides both cryopreserved human adipose mesenchymal stem cells (MSCs) and low-serum expansion media that is recommended for optimal growth and expansion of adipose MSCs. (
  • Researchers have seen that spheroids display enhanced anti-inflammatory, tissue regenerative, and reparative responses, as well as better post-transplant survival of mesenchymal stem cells. (
  • cells (BBB) and aortic endothelial cells (control). (
  • 'All opinons are MINE and MINE alone' A friend of mine needs help culturing Mouse brain capillary endothelial cells--they won't attach and die soon after. (
  • Due to the heterogeneous nature of animal organs, primary cell cultures potentially contain more than one type of cell. (
  • Primary cell cultures are sometimes preferred over continuous cell lines in experimental systems. (
  • Use of such cells is sometimes easier than use of primary cell cultures, especially for generation of stably transfected clones. (
  • Rodent primary cell cultures form continuous cell lines relatively easily, either spontaneously or following exposure to a mutagenic agent. (
  • In contrast, human primary cell cultures rarely, if ever, become immortal in this way and require additional genetic manipulation to form a continuous cell line. (
  • With the exception of some derived from tumors, most primary cell cultures have limited lifespan. (
  • Join us in February 2019, as we look at the latest developments in the 3D Cell Culture field. (
  • This webinar will introduce basic concepts of cell culture and provide tips on how to succeed in culturing mammalian cells. (
  • The book is written for Master Students and PhD Candidates in biotechnology, tissue engineering and biomedicine working with mammalian, and specifically human cells. (
  • They play a leading role not only in muscle cells: Actin filaments are one of the most abundant proteins in all mammalian cells. (
  • Depending on their origin, animal cells grow either as an adherent monolayer or in suspension. (
  • Adherent cells are anchorage-dependent and propagate as a monolayer attached to the cell culture vessel. (
  • Most cells require a surface or an artificial substrate (adherent or monolayer culture) whereas others can be grown free floating in culture medium (suspension culture). (
  • The scaffolds were then transferred into the abdominal cavities of rats, where conditions were favorable for the cells in the scaffolds to interact and develop. (
  • We look forward to meeting you at 3D Cell Culture 2018 in Freiburg! (
  • In Vitro Cell. (
  • To create tissue-specific structures and function in vitro, cell models have to be translated into the third dimension. (
  • Because of this, sterile cell culture work should be conducted in a fume hood with disinfected surfaces and devices. (
  • Perform general cell culture procedures more economically with Thermo Scientific™ BioLite Cell Culture Treated Dishes, guaranteed to be sterile, non-pyrogenic and non-cytotoxic. (
  • sterile, recommended for immunohistochemistry of cell lines growing in suspension, Poly-D-Lysine coated, glass slides with polystyrene vessel, lid and safety removal tool. (
  • Sterile and ready to use for straining stem and primary cell samples. (
  • NanoLogix , a company out of Ohio, is trying to solve both of these by commercializing their cost-effective Petri dish membrane that supercharges a traditional culture to allow earlier detection of viable microorganisms. (
  • After subsequent checks at 48 and 72 hours, the broth transferred to the glass bottle, two conical tubes and the cell culture petri dish are all uncontaminated! (
  • 2D cell culture has had an extraordinary place in cell culture history and will remain a viable cell culture model for many uses. (
  • contains 1 million viable cells and 500ml of optimized media. (
  • Due to strict regulatory demands [ 18 , 19 ], the development of a suitable storage method will be essential to enable the transportation of viable cell constructs from centralized laboratories to operating theatres [ 18 ]. (
  • Physiological processes are guided by interactions between cells and their extracellular matrix, the proteins and polysaccacharides that cells secrete into their environment to support tissue structure and survival. (
  • We would argue that 3D cell culture models have been used for many years in basic research and disease modeling, notably in cancer research-this was, after all, one of the original applications of Corning® Matrigel®, a naturally occurring extracellular matrix for us in 3D cell culture. (
  • The invention is based, at least in part, on the discovery that cycling the cultures in this manner optimizes the formation of extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue. (
  • d) repeating steps (b) and (c) as necessary to produce extracellular matrix proteins in the three dimensional stromal culture. (
  • Researchers globally are realizing the potential of 3D cell culture for various applications, including testing and discovering new drugs to treat cancer, organ-on-chip models to study the human physiology in an organ specific context, and 3D cell printing to produce organ models. (
  • Researchers are now bridging the gap between natural and synthetic gels by combining well-characterized synthetic materials with biomimetic cues to support physiologically relevant cell-gel interactions (see the figure). (
  • In a paper in the American Chemical Society's journal Langmuir*, NIST researchers detail a microfluidics technique to culture neuronal cells in relative isolation on a variety of cell-culture surfaces, and to pattern the cells on the surface to study the effects of geometry on cell development. (
  • The researchers created sphere-shaped cell cultures to allow the cells to develop more naturally. (
  • The ClearT2 technique was found to be the most efficient, as it allowed the researchers to see fluorescing cells at all depths of focus without changing the size of the tissue. (
  • Scaffold-free, self-assembled adult hippocampal neural stem cells that were 100 µm in diameter could be optically cleared and imaged using ClearT2 while retaining their size, the researchers wrote in the study. (
  • Today, during the 85thth General Session of the International Association for Dental Research, a team of researchers from the Institute of Medical Science, the University of Tokyo (Japan), reports on a new technique for culturing cells that have the capacity to produce enamel. (
  • From product selection guides to application notes to protocols, our online resource library is available anytime you need to facilitate your cell culture applications. (
  • Rely on innovative vessels from Corning for quality and consistency in your cell culture applications. (
  • It is available with three different culture chambers (QV500, QV600, and QV900) to support a wide range of applications, including submerged cell culture, coculture, and modeling of air-liquid and liquid-liquid interfaces. (
  • Corning™ Untreated 245mm Square Low Profile BioAssay Dishes provide a superior option for applications where cell attachment is not desired. (
  • Using Nunc Glass Bottom Dishes in your research applications gives you the flexibility to convert your culture device into an imaging device. (
  • Cell culture is a fundamental technique for a variety of applications. (
  • And in therapeutics, 3D cell culture promises to realize several exciting applications, from patient-specific drug-efficacy screening to the manufacturing of bioprinted organs. (
  • GEN: Are 3D cell culture models as strongly focused as ever on drug safety testing, or are they finding new applications? (
  • Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. (
  • Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online.Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. (
  • Click here to register for free and to view the entire webinar: Since 2008 Kaiser Optical has worked closely with industry leading bioprocessing companies to develop a Raman solution enabled to deliver in situ, real-time measurement to a host of bioprocessing applications including but not limited to cell culture, fermentation, and purification. (
  • Since our first symposium in 2009, the 3D Cell Culture conference series has developed into a 'place to be' for experts active in this field, and will highlight successful applications and newest developments. (
  • Success stories of 3D culture applications will be presented as well as newest technologies and innovations. (
  • Relevant disease models, applications of 3D models in clinic and industry, predictive cell models for compound characterization and enabling technologies will be covered. (
  • This textbook provides an overview on current cell culture techniques, conditions and applications, it specifically focuses on human cell culture. (
  • Applications such as transfection or immunofluorescence staining or live-cell imaging can be conveniently performed. (
  • Optimization of cell culture conditions is necessary to ensure that cells are healthy and in optimal condition for downstream applications. (
  • These disposable, space saving containers are specifically designed for optimal plant cell culture applications. (
  • The 'Recombinant Cell Culture Supplements' market can be divided based on product types and It's sub-type, major applications and Third Party usage area, and important regions. (
  • While a number of procedures have been used to enrich the cell population of interest, many scientists have resorted to cloning of cells in order to insure the purity of cell cultures. (
  • A team of scientists led by Prof. Antonella Consiglio from the IDIBELL and the University of Barcelona, and Prof. Angel Raya from the Center of Regenerative Medicine of Barcelona have discovered that defective versions of human brain cells called astrocytes are linked to the buildup of a toxic protein that is one the hallmarks of Parkinson's disease. (
  • The µ-Dish 35-mm Quad is the ideal solution for scientists who conduct simultaneous multiplex analysis of different cell lines, or apply distinct experimental conditions. (
  • Scientists have also learned much about cancer initiation and progression through the use of cells in culture. (
  • Technology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs. (
  • For this reason, it is exciting to consider the prospect of artificially growing enamel, or even whole teeth, using culturing and transplantation techniques. (
  • With the demonstration of long-term survival of RPE cell transplants both in various animal models and in humans [ 8 - 13 ], transplantation offers the prospect of a single intervention cure. (
  • The transplantation of RPE grafts enables appropriate implantation and orientation of an organized RPE cell layer in the retina [ 5 , 14 , 15 ] and circumvents several of the complications associated with the use of RPE cell suspensions [ 6 , 8 , 15 , 16 ]. (
  • The cover can be snapped on tight, for long-term culture, or loosely fitted during acclimation to external conditions prior to transplantation. (
  • Primary cells, finite cultures, and continuous cell lines differ in their proliferative potential (see below). (
  • Finite cell cultures are formed after the first subculturing (passaging) of a primary cell culture. (
  • The proliferative potential of some human finite cell cultures can be extended by introduction of viral transforming genes (e.g., the SV40 transforming-antigen genes). (
  • The phenotype of these cultures is intermediate between finite cultures and continuous cultures. (
  • Finite cell cultures will eventually either die out or acquire a stable, heritable mutation that gives rise to a continuous cell line that is capable of unlimited proliferative potential. (
  • An improved technique for culturing cells, developed at the National Institute of Standards and Technology (NIST), may enable new, fundamental insights into the behavior of neuronal cells. (
  • Neuronal cells, which make up the central nervous system in mammals, are both particularly important and particularly hard to culture. (
  • They are highly specialized and choosy about their environment--normally they only survive and develop when cultured on a layer of non-neuronal "glial" cells that provide cellular support services. (
  • There are usually far more glial cells than neuronal cells, which makes it hard to image neuronal cells and measure their activity against the glial background. (
  • Properly selected, the PEM coating convinces the neuronal cells that they're in a good environment to attach, develop and produce the characteristic neuron projections and synapses, all without a glial layer. (
  • Neuronal cells will largely confine themselves to the pattern, enabling a variety of cell-geometry experiments, such as measuring the maximum gap between lines that can be bridged by neural axons and dendrites. (
  • Used in the growth of neuronal cells from hippocampus, cortex and otregions of the brain. (
  • The confluence of morphologic, genetic and biochemical approaches laid the foundations for study in this area, and they continue to strengthen our understanding of this essential aspect of cell structure and function. (
  • 3D cell culture, besides being more convenient than animal models (and relatively free of ethical issues), permits extraordinary control-from the genetic level upward. (
  • This test is intended for tissue samples that do not have any accompanying cytogenetic test order (e.g. chromosome analysis or microarray analysis) but needs to be cultured for other molecular genetic or biochemical testing (e.g. (
  • This test is intended for amniotic fluid samples that does not have any accompanying cytogenetic test order (e.g. chromosome analysis) but cell culture is desired for other molecular genetic or biochemical testing, such as familial mutation analysis, DNA isolation and storage, and send-out testing for molecular genetic/biochemical testing to be done on cultured amniocytes. (
  • Genetic and epigenetic properties of mouse male germline stem cells during long-term culture," Development , vol. 132, no. 18, pp. 4155-4163, 2005. (
  • ECACC is one of the first collections of authenticated cell cultures worldwide and now holds cell lines from 45 species including 50 tissue types, 300 HLA types, over 800 genetic disorders and roughly 450 monoclonal antibodies. (
  • 3. A method according to claim 1, in which the method comprises supplementing a basal medium with a supplement comprising the thymidine family member to produce the cell culture medium. (
  • Airway Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. (
  • Support non-enzymatic harvesting of adherent cells for preservation of cell viability and surface proteins with Thermo Scientific™ Nunc™ Dishes with UpCell™ Surface. (
  • Finally, many standard techniques for analyzing proteins and protein distributions are more difficult to perform, because they require isolation of the cells from the matrix. (
  • The advantages of synthetic gels include their consistent composition and predictable manipulation of properties, but they lack functional sites to interact with soluble or cell-surface proteins. (
  • There have been some breakthroughs on 1, last I heard a group had shown you can just culture with modified proteins to induce pluripotency. (
  • Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses. (
  • We describe a method of growing an animal cell in a culture medium, in which the culture medium comprises an elevated concentration of a thymidine family member, in which the growth or viability of the animal cell is increased as a result of the elevated concentration of the thymidine family member in the cell culture medium. (
  • 18. A method according to claim 1, in which cell growth or cell viability is enhanced by at least 50% compared to growth in a cell culture medium without an elevated concentration of thymidine family member. (
  • 19. A method according to claim 1, in which apoptosis of the animal cell in the cell culture medium is reduced to enhance cell viability. (
  • For the majority of isolated primary cells, they undergo the process of senescence and stop dividing after a certain number of population doublings while generally retaining their viability (described as the Hayflick limit). (
  • Please view our archived webinar and learn how the new RAFT™ 3D Cell Culture System can elevate your cell culture. (
  • 4. The method of claim 1 , wherein the human umbilical cord feeder cells consist of 100% human umbilical cord fibroblast feeder cells. (
  • 7. The method of claim 6 , wherein the human umbilical cord feeder cells consists essentially of the human umbilical cord fibroblast feeder cells. (
  • It is: Kruk, P.A. and Auersperg, N. Percoll Centrifugation Eliminates Mold Contaminants from Cell Cultures. (
  • Look to Corning for the right surface for every cell-from biological to synthetic-to provide a cell culture environment that meets your research requirements. (
  • These culture systems heavily rely on optical imaging modalities to provide biological information. (
  • Each culture room is equipped with two biological safety cabinets, a stack of three large incubation shakers, a refrigerated tabletop centrifuge, a basic compound microscope as well as a variety of incubators, water baths and shakers. (
  • Three-dimensional (3D) cell culture overcomes many shortcomings of two-dimensional (2D) culture, mainly through the introduction of biological relevance. (
  • This comprises advanced 3D cell culture models for efficacy and safety studies, enabling technologies to create and analyze biological reactions as well as their successful implementation in compound de-risking. (
  • Operating as exquisitely sensitive filters, these "ion channels" play a critical role in biological functions such as muscle contraction and the firing of brain cells. (
  • Culturing Life points to biological research practices in the lab, but at the same time it refers to our contemporary culture that, in more than one way, refers to and relies on developments in the life sciences. (
  • 3D cell culture captures structural and organizational variations, and hence physiological variations, that can help it represent real, living tissue far better than 2D cell culture ever could. (
  • Get updated about 3D cell cultures as predictive, physiological relevant model systems! (
  • The main functions of the DSMZ plant cell culture collection are to collect and deliver plant cell cultures with relevance to biotechnology, fundamental and applied research. (
  • Glycosylation of 2-, 3-, and 4-fluorophenols using plant cell cultures of Nicotiana tabacum was investigated to elucidate their potential to metabolize these compounds. (
  • Hamada, H. Glycosylation of Fluorophenols by Plant Cell Cultures. (
  • All plant cell cultures in the PCCL are categorized into three levels of growth, High , Medium and Low . (
  • Schwann cells in peripheral nerves receive molecular signals from axons to coordinate the process of myelination. (
  • A manual providing all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis and gene transfer. (
  • Our current understanding at the molecular level of this modulatory process and its relevance to T cell tolerance are reviewed. (
  • The expandable vessel promotes the growth of large numbers of adherent or suspension cells by providing the volumes of nutrient medium and gaseous exchange. (
  • Adherent primary cells are particularly susceptible to contact inhibition, that is, they will stop growing when they have reached confluency. (
  • Any info on where these can be obtained, along with any special conditions for culturing, would be gratefully received. (
  • Cells are cultured in controlled conditions including temperature, gas exchange, pH, and culture media (energy and nutrients). (
  • Cell culture is the process by which cells are grown under controlled conditions. (
  • ARPE-19 cells were cultured under standard conditions and stored in HEPES-buffered MEM at nine temperatures (4°C, 8°C, 12°C, 16°C, 20°C, 24°C, 28°C, 32°C, and 37°C) for seven days. (
  • When operated in dual mode, culture conditions in the separate Cellbag bioreactors are independently controlled by two ReadyToProcess CBCU units. (
  • To this end, a murine cell line will be used to assess toxicity of materials used as treatments of neuropathic conditions. (
  • The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. (
  • Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. (
  • After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. (
  • The lifespan of most cells is genetically determined, but some cell culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (
  • Moreover, development of culture techniques ensuring specificity and specialty growth media, and rising application of high-end technologies to scale up culture production are expected to bolster the market growth over the following decade. (
  • Professor Przyborski is developing new and innovative ways to manage the growth and function of cultured cells. (
  • Heterogeneity in growth characteristics of satellite cell populations. (
  • Interaction of insulin-like growth factor I with turkey satellite cells and satellite cell-derived myotubes. (
  • Variation in response to growth factor stimuli in satellite cell populations. (
  • In addition, the third floor culture room is equipped with one lighted incubation shaker and a Coy gloveless anaerobic chamber which was converted into a light chamber (owned by the Martin lab) for the growth of photosynthetic organisms. (
  • The New Brunswick Innova 4430 incubation shakers are used for the growth of microbial cultures in liquid media. (
  • Cell culture is set to see huge increases in growth including the recent advances in techniques such as 3D cell culture. (
  • Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. (
  • How do I know the minimum of cells for a specific cell line that give a quick entry into log phase growth? (
  • Plate different dilutions of your cell line and see were the cut off point is, usually theres a treshold were the cells are suddenly to sparsely plated to give rise to normal growth. (
  • The Ph+ feature avoids any meniscus effect and facilitates homogenous cell growth. (
  • 2. The cell growth apparatus according to claim 1, wherein each compartment comprises opposing gas permeable, liquid impermeable surfaces. (
  • 3. The cell growth apparatus according to claim 1, wherein each compartment comprises a plurality of gas permeable, liquid impermeable surfaces which form the expandable walls of the compartment. (
  • 4. The cell growth apparatus according to claim 1, wherein the gas permeable, liquid impermeable surfaces are selected from the group consisting of polystyrene, polypropylene, polyethylene, polycarbonate, silicone rubber, fluoroethylenepropylene copolymer and a combination thereof. (
  • 6. The cell growth apparatus according to claim 1, wherein the spacer material is selected from the group consisting of a woven mesh and a planar sheet comprising protruding support features. (
  • 7. The cell growth apparatus according to claim 1, further comprising a support structure external to the compartments. (
  • 8. The cell growth apparatus according to claim 7, wherein the support structure comprises a compartment frame perimetrically surrounding each of the compartments for supporting the gas permeable, liquid impermeable surfaces. (
  • 9. The cell growth apparatus according to claim 7, wherein the support structure comprises a top platen and a bottom platen confining the plurality of compartments there-between. (
  • 10. The cell growth apparatus according to claim 7, wherein the support structure is optically transparent. (
  • 11. The cell growth apparatus according to claim 9, wherein the support structure further comprises at least one rod inserted through a supported hole in at least one of the compartments and attached to the top platen and the bottom platen. (
  • 12. The cell growth apparatus according to claim 10, wherein the rod is telescoping to form an expandable support structure. (
  • 13. The cell growth apparatus according to claim 1, wherein the interconnecting passageway comprises ports in the gas permeable, liquid impermeable surface of each compartment. (
  • From cell preparation and growth to investigation and analysis, our comprehensive portfolio provides the quality and consistency to ensure the validity of your results. (
  • Different cell types vary greatly with respect to their growth behavior and nutritional requirements. (
  • Growth, Nutrition, and Metabolism of Cells in Culture, Volume 1, summarizes the state of knowledge of the growth, nutrition, and metabolism of various types of cell cultures. (
  • Dispersed cells are then transferred to a suitable growth medium and allowed to attach to the surface of culture flasks. (
  • Cell culture requires careful attention to the growth medium to ensure cells are given all the components they require to grow. (
  • Often the culture medium requires growth factors or hormones to stimulate growth. (
  • Media used to propagate cells are rich in nutrients and, therefore, support growth of a multitude of organisms. (
  • Designed to improve usability while maintaining the same environment for cell growth as previous designs. (
  • Designed to support cell culture growth and biomanufacturing processes. (
  • however, only the white cells are capable of growth in culture. (
  • Alternatively, pieces of tissue can be placed in growth media, and the cells that grow out are available for culture. (
  • Aside from temperature and gas mixture, the most commonly varied factor in culture systems is the cell growth medium. (
  • In the absence of the latter signal, the T cell makes only a partial response and, more importantly, enters an unresponsive state known as clonal anergy in which the T cell is incapable of producing its own growth hormone, interleukin-2, on restimulation. (
  • Tissue-level effects can now be probed in vitro thanks to recently developed systems of three-dimensional (3D), multicellular, "organotypic" cell cultures, which mimic functional responses of living tissue. (
  • In practice the term "cell culture" has come to refer to the culturing of cells derived from multicellular eukaryotes, especially animal cells. (
  • Interestingly, spheroids derived from stem cells grown in 3D models show improved "stemness," that is, characteristics that may lead to increased efficacy in regenerative medicine. (
  • The book is based on lectures, seminars and practical courses in stem cells, tissue engineering, regenerative medicine and 3D cell culture held at the University Natural Resources and Life Sciences Vienna BOKU and the Gottfried Wilhelm Leibniz University Hannover and therefore delivers in a compact and clear way important theoretical as well as practical knowledge to advanced graduate students on cell culture techniques and the current status of research. (
  • Day 4: Sub-confluent ES cell colonies are washed twice with PBS and treated with trypsin to obtain a single cell suspension. (
  • Hematopoietic cells (derived from blood, spleen, or bone marrow) as well as some transformed cell lines and cells derived from malignant tumors can be grown in suspension. (
  • The PCCL database has captured Order, Family, Genus and Species information for each cell line allowing users to search at different taxonomic levels. (
  • For example, entering the order Caryophyllales in the search bar returns all cultures in that order. (
  • New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. (
  • The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience. (
  • A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. (
  • They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development. (
  • It covers different types of animal cell cultures, considerations for cell culture, and cell culture protocols. (
  • And each different cell type seems to differentiate in different ways. (
  • Isolation and characterization of myogenic satellite cells derived from the muscular dystrophic hamster. (
  • The ability to simultaneously image multiple biomolecules in biologically relevant three-dimensional (3D) cell culture environments would contribute greatly to the understanding of complex cellular mechanisms and cell-material interactions. (
  • First, even in two-dimensional culture, heterogeneities exist in the cellular microenvironment, and these will only be further exaggerated in three-dimensional gels. (
  • It will enable simultaneous electrical-optical cell monitoring and will be used to characterise communication between cell populations, with modes of cellular communication teased apart by systematically controlling cell-cell interactions. (
  • Are you sure you want to remove Mycoplasma Infection of Cell Cultures (Cellular Senescence and Somatic Cell Genetics) from your list? (
  • Of key importance were specifically designed flasks and appropriate nutrient media, as well as the meticulous handling ("washing") of cells. (
  • When cells have grown (by dividing) to cover the flasks' surface, the process of enzymic dissociation can be repeated and the cells replanted to additional flasks. (
  • Corning™ U-Shaped Cell Culture Flasks have an ergonomic shape that reduces the number of corners, improves cell scraping and allows the use of a larger pipet. (
  • Preferably, the cell culture medium comprises a semi-solid medium, which is a serum free or chemically defined medium. (
  • 10. A method according to claim 1, in which the cell culture medium comprises an elevated concentration of both thymidine and uridine. (
  • 11. A method according to claim 1, in which the cell culture medium comprises thymidine at 0.24 mg/l. (
  • 13. A method according to claim 1, in which the cell culture medium further comprises conditioned medium. (
  • 5. The method of claim 1 , wherein the culture system further comprises nicotinamide. (
  • The course is designed for life science and engineering graduates new to stem cell culture or for more experienced individuals new to working with iPSC or hES cell lines. (
  • Delegates will leave the module with practical knowledge to propagate, maintain and characterize feeder and feeder-free stem cell lines. (
  • Primary cell culture is generally more difficult than culture of continuous cell lines. (
  • In addition, cell lines cultured for extended periods of time can undergo phenotypic and genotypic changes that can lead to discrepancies when comparing results from different laboratories using the same cell line. (
  • Furthermore, many cell types are not available as continuous cell lines. (
  • Cancer cell lines, which can grow indefinitely in culture, also retain long telomeres. (
  • Three dropdown menus display names for Family, Genus and the Accepted Binomial populated for all cell lines contained in the collection. (
  • Numerous cell lines are well established as representative of particular cell types. (
  • The European Collection of Authenticated Cell Cultures houses and supplies cell lines. (
  • In Jun 2020, ThermoGenesis announced the launch of X-SERIES cell processing products at the commercial level. (
  • The cell culture market is set to be worth £4.5 billion by 2020 driven by advances in pharma and biotech. (