Cell Culture Techniques: Methods for maintaining or growing CELLS in vitro.Batch Cell Culture Techniques: Methods for cultivation of cells, usually on a large-scale, in a closed system for the purpose of producing cells or cellular products to harvest.Culture Techniques: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.Bacteriological Techniques: Techniques used in studying bacteria.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Organ Culture Techniques: A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)Tissue Culture Techniques: A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Evaluation Studies as Topic: Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.Blood: The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.Microbiological Techniques: Techniques used in microbiology.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Bacteria, AerobicColony Count, Microbial: Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Feces: Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.Centrifugation: Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Pharynx: A funnel-shaped fibromuscular tube that conducts food to the ESOPHAGUS, and air to the LARYNX and LUNGS. It is located posterior to the NASAL CAVITY; ORAL CAVITY; and LARYNX, and extends from the SKULL BASE to the inferior border of the CRICOID CARTILAGE anteriorly and to the inferior border of the C6 vertebra posteriorly. It is divided into the NASOPHARYNX; OROPHARYNX; and HYPOPHARYNX (laryngopharynx).Agar: A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.Bacteroides: A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.Specimen Handling: Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Fungi: A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.Cell SeparationReagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Methods: A series of steps taken in order to conduct research.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Anaerobiosis: The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Sputum: Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.Bacterial Infections: Infections by bacteria, general or unspecified.Sepsis: Systemic inflammatory response syndrome with a proven or suspected infectious etiology. When sepsis is associated with organ dysfunction distant from the site of infection, it is called severe sepsis. When sepsis is accompanied by HYPOTENSION despite adequate fluid infusion, it is called SEPTIC SHOCK.Hemolysis: The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Staphylococcus: A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.Coculture Techniques: A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.Tissue Engineering: Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.Colony-Forming Units Assay: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Water Microbiology: The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Immunoenzyme Techniques: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Angiotensin II increases the release of endothelin-1 from human cultured endothelial cells but does not regulate its circulating levels. (1/8613)

We investigated the effect of angiotensin II on endothelin-1 secretion in vitro and in vivo. In vivo, angiotensin II was given intravenously to 23 essential hypertensive and 8 control subjects according to different protocols: Study A, 1.0 ng x min-1 x kg-1 and 3.0 ng x min-1 x kg-1 angiotensin II for 30 min each; Study B, 1.0 ng x min-1 x kg-1 and 3.0 ng x min-1 x kg-1 angiotensin II for 120 min each; Study C, 3.0 ng x min-1 x kg-1 angiotensin II for 30 min followed by a dose increment of 3.0 ng x min-1 x kg-1 every 30 min until mean blood pressure levels increased by 25 mmHg; Study D, 1.0 ng x min-1 x kg-1 followed by 3.0 ng x min-1 x kg-1 angiotensin II for 60 min each on two different NaCl diets (either 20 mmol NaCl/day or 220 mmol NaCl/day, both for 1 week). In all in vivo studies neither plasma nor urine endothelin-1 levels changed with angiotensin II infusion. In contrast, angiotensin II (10(-9), 10(-8), 10(-7) mol/l) stimulated endothelin-1 secretion from cultured human vascular endothelial cells derived from umbilical cord veins in a time- and dose-dependent manner. The in vitro angiotensin II effects were abolished by candesartan cilexetil, an inhibitor of the membrane-bound AT1 receptor, and also by actinomycin D, an RNA synthesis inhibitor, and cycloheximide, a protein synthesis inhibitor, indicating that endothelin-1 release depended on AT1 receptor subtype and de novo protein synthesis. Our findings indicate that angiotensin II regulates endothelin-1 release by cultured endothelial cells through an AT1 receptor-dependent pathway, but does not influence circulating endothelin-1 levels in vivo.  (+info)

A technique for dual determination of cytotoxic and helper lymphocyte precursor frequency by a miniaturized dye release method. (2/8613)

Helper (HTLPf) and cytotoxic (CTLPf) lymphocyte precursor frequency assays are increasingly used in bone marrow stem cell and organ transplant compatibility testing. Current techniques require large cell numbers and radioisotopes. To improve the technique, we developed a miniaturized fluorescent read-out combined HTLPf/CTLPf limiting dilution assay. The assay requires only 5 x 10(6) stimulators, 2 x 10(6) responders and 0.24 x 10(6) target cells in Terasaki plates (40 microl/well). For the HTLPf, culture supernatants from each well were assayed for IL-2 production. The IL-2-dependent proliferation of the mouse 9.12 cell line was detected by a semi-automated fluorescent dye technique. After addition of rhIL-2 (recombinant human IL-2) on days 3 and 7, CTLPs were detected on day 10 by measuring the lysis of dye-labeled targets. Results were comparable to standard radioisotope-based techniques. The assay had a coefficient of variation of approximately 30%. The assay detected helper CD4 cells, pure cytotoxic CD8, helper CD8 cells and helper/cytotoxic CD8 cells. Discrimination was demonstrated between HLA-matched related and non-related pairs. The ease of testing and small cell numbers required should facilitate further evaluation of HTLPf and CTLPf for compatibility testing in unrelated donor transplantation and monitoring immune responses following adoptive transfer of lymphocytes.  (+info)

Generation and characterization of aggrecanase. A soluble, cartilage-derived aggrecan-degrading activity. (3/8613)

A method was developed for generating soluble, active "aggrecanase" in conditioned media from interleukin-1-stimulated bovine nasal cartilage cultures. Using bovine nasal cartilage conditioned media as a source of the aggrecanase enzyme, an enzymatic assay was established employing purified aggrecan monomers as a substrate and monitoring specific aggrecanase-mediated cleavage products by Western analysis using the monoclonal antibody, BC-3 (which recognizes the new N terminus, ARGS, on fragments produced by cleavage between amino acid residues Glu373 and Ala374). Using this assay we have characterized cartilage aggrecanase with respect to assay kinetics, pH and salt optima, heat sensitivity, and stability upon storage. Aggrecanase activity was inhibited by the metalloprotease inhibitor, EDTA, while a panel of inhibitors of serine, cysteine, and aspartic proteinases had no effect, suggesting that aggrecanase is a metalloproteinase. Sensitivity to known matrix metalloproteinase inhibitors as well as to the endogenous tissue inhibitor of metalloproteinases, TIMP-1, further support the notion that aggrecanase is a metalloproteinase potentially related to the ADAM family or MMP family of proteases previously implicated in the catabolism of the extracellular matrix.  (+info)

The cellular ecology of progressive neoplastic transformation: a clonal analysis. (4/8613)

A comparison was made of the competence for neoplastic transformation in three different sublines of NIH 3T3 cells and multiple clonal derivatives of each. Over 90% of the neoplastic foci produced by an uncloned transformed (t-SA') subline on a confluent background of nontransformed cells were of the dense, multilayered type, but about half of the t-SA' clones produced only light foci in assays without background. This asymmetry apparently arose from the failure of the light focus formers to register on a background of nontransformed cells. Comparison was made of the capacity for confluence-mediated transformation between uncloned parental cultures and their clonal derivatives by using two nontransformed sublines, one of which was highly sensitive and the other relatively refractory to confluence-mediated transformation. Transformation was more frequent in the clones than in the uncloned parental cultures for both sublines. This was dramatically so in the refractory subline, where the uncloned culture showed no overt sign of transformation in serially repeated assays but increasing numbers of its clones exhibited progressive transformation. The reason for the greater susceptibility of the pure clones is apparently the suppression of transformation among the diverse membership that makes up the uncloned parental culture. Progressive selection toward increasing degrees of transformation in confluent cultures plays a major role in the development of dense focus formers, but direct induction by the constraint of confluence may contribute by heritably damaging cells. In view of our finding of increased susceptibility to transformation in clonal versus uncloned populations, expansion of some clones at the expense of others during the aging process would contribute to the marked increase of cancer with age.  (+info)

Micronucleus test using cultured new born rat astrocytes. (5/8613)

Micronuclei is induced in cytoplasm as a consequence of the formation of chromosomal fragments or remaining chromosomes during cell division by the cause of clastogens or spindle poisons, and is used as an indicator of genotoxicity screening tests. There are few short-term genotoxicity screening tests using brain cells. We attempted to establish a new in vitro micronucleus test (MN test) system by use of central nervous system cells. Primary cultured astrocytes were prepared from newborn male Sprague-Dawley (SD) rats. In growth curve of astrocytes, doubling time was determined to be 31 h. In time study, the highest frequency of micronuclei was observed at 48 h, 72 h and 6 h-exposure-66 h-recovery by vincristine (VCR), mitomycin C (MMC) without metabolic activation system and cyclophosphamide (CPM) with metabolic activation system, respectively. Dose-response relationships between micronucleus frequency and concentrations of MMC, VCR and CPM were observed, respectively. It is suggested that the in vitro MN test using new born rat-astrocytes could be used as a screening test of environmental and occupational genotoxic chemicals in the central nervous system cells.  (+info)

In-vitro fertilization and culture of mouse embryos in vitro significantly retards the onset of insulin-like growth factor-II expression from the zygotic genome. (6/8613)

In this study, the effect of in-vitro fertilization (IVF) and culture of mouse embryos in vitro on the normal expression of insulin-like growth factor-II (IFG-II) ligand and receptor was examined. The expression of IGF-II increased in a linear fashion at least up to the 8-cell stage of development. IGF-II expression in embryos collected fresh from the reproductive tract was significantly (P < 0.001) greater than in embryos fertilized in the reproductive tract and cultured in vitro (in-situ fertilized: ISF), and its expression was further reduced (P < 0.001) in IVF embryos at all development stages tested. The expression of IGF-II was significantly (P < 0.001) lower when embryos were cultured individually in 100 microl drops compared with culture in groups of 10 in 10 microl drops of medium. The addition of platelet activating factor to culture medium partially overcame this density-dependent decline of expression. Culture of ISF and IVF zygotes also caused the onset of new IGF-II mRNA transcription from the zygotic genome to be significantly (P < 0.001) retarded, until at least the 8-cell stage of development. This effect was greater (P < 0.05) for IVF than for ISF embryos. Neither IVF nor culture had any obvious effect on IFG-II/mannose-6-phosphate receptor (IGF-IIr) mRNA expression.  (+info)

Isolation and characterization of a new human breast cancer cell line, KPL-4, expressing the Erb B family receptors and interleukin-6. (7/8613)

A new human breast cancer cell line, KPL-4, was recently isolated from the malignant pleural effusion of a breast cancer patient with an inflammatory skin metastasis. This cell line can be cultured under serum-free conditions and is tumorigenic in female athymic nude mice. Flow cytometric analysis revealed the expression of Erb B-1, -2 and -3. Dot blot hybridization showed a 15-fold amplification of the erb B-2. Reverse transcription-polymerase chain reaction analysis showed a detectable level of mRNA expression of all the Erb B family receptors. In addition, all the receptors were autophosphorylated under a serum-supplemented condition. Unexpectedly, transplanted KPL-4 tumours induced cachexia of recipient mice. A high concentration of interleukin-6 (IL-6) was detected in both the culture medium and the serum of mice. The weight of tumours significantly correlated with the serum IL-6 level. The antiproliferative effect of a humanized anti-Erb B-2 monoclonal antibody, rhuMAbHER2, was investigated. This antibody significantly inhibited the growth of KPL-4 cells in vitro but modestly in vivo. Loss of mouse body weight was partly reversed by rhuMAbHER2. These findings suggest that KPL-4 cells may be useful in the development of new strategies against breast cancer overexpressing the Erb B family receptors and against IL-6-induced cachexia.  (+info)

Generation and characterization of human smooth muscle cell lines derived from atherosclerotic plaque. (8/8613)

The study of atherogenesis in humans has been restricted by the limited availability and brief in vitro life span of plaque smooth muscle cells (SMCs). We describe plaque SMC lines with extended life spans generated by the expression of the human papillomavirus (HPV)-16 E6 and E7 genes, which has been shown to extend the life span of normal adult human aortic SMCs. Resulting cell lines (pdSMC1A and 2) demonstrated at least 10-fold increases in life span; pdSMC1A became immortal. The SMC identity of both pdSMC lines was confirmed by SM22 mRNA expression. pdSMC2 were generally diploid but with various structural and numerical alterations; pdSMC1A demonstrated several chromosomal abnormalities, most commonly -Y, +7, -13, anomalies previously reported in both primary pdSMCs and atherosclerotic tissue. Confluent pdSMC2 appeared grossly similar to HPV-16 E6/E7-expressing normal adult aortic SMCs (AASMCs), exhibiting typical SMC morphology/growth patterns; pdSMC1A displayed irregular cell shape/organization with numerous mitotic figures. Dedifferentiation to a synthetic/proliferative phenotype has been hypothesized as a critical step in atherogenesis, because rat neonatal SMCs and adult intimal SMCs exhibit similar gene expression patterns. To confirm that our pdSMC lines likewise express this apparent plaque phenotype, osteopontin, platelet-derived growth factor B, and elastin mRNA levels were determined in pdSMC1A, pdSMC2, and AASMCs. However, no significant increases in osteopontin or platelet-derived growth factor B expression levels were observed in either pdSMC compared with AASMCs. pdSMC2 alone expressed high levels of elastin mRNA. Lower levels of SM22 mRNA in pdSMC1A suggested greater dedifferentiation and/or additional population doublings in pdSMC1A relative to pdSMC2. Both pdSMC lines (particularly 1A) demonstrated high message levels for matrix Gla protein, previously reported to be highly expressed by human neointimal SMCs in vitro. These results describe 2 novel plaque cell lines exhibiting various features of plaque SMC biology; pdSMC2 may represent an earlier plaque SMC phenotype, whereas pdSMC1A may be representative of cells comprising an advanced atherosclerotic lesion.  (+info)

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Haycock, John W. (Herausgeber) 3D Cell Culture Methods and Protocols Reihe: Methods in Molecular Biology 695 9781607619833 1607619830 A10082632 A10082632
If you have a question about this talk, please contact Ilana Spilka.. Increasing evidence suggests that the tumor heterogeneity is rooted in the existence of a very small sub-population of stem-like cells (CSCs) within the population of tumor cells. Since cancer takes many years to grow, it is important to develop in vitro models to study tumor initiation and progression. In an effort to regulate the cancer cell microenvironment, my laboratory has merged material synthesis, microscale technologies, and cancer biology to develop an engineered 3D matrix to delineate the role of individual factors in the tumor microenvironment on CSC maintenance, self-renewal, and differentiation. I will demonstrate in my talk that engineered matrices can enrich the CSC subpopulation of cancer cells which is potentially useful in cancer drug screening and personalized cancer treatment.. This talk is part of the Three-dimensional cell culture: Innovations in tissue scaffolds and biomimetic systems series.. ...
Collaborating with the Tokyo University Industrial Technology center, KISCO developed original cell culture substrates based on diX coating technology.. ...
Researchers are increasingly turning to 3D cell culture techniques for their studies, due to the improved physiological relevance of the cellular environment. TAP Biosystems has developed a novel 3D cell culture technique, RAFT (Real Architecture for 3D Tissue), which allows researchers to culture cell types of their choice in an in vivo like environment. In this poster it is shown that the Tecan EVO freedom can be used to reliably automate 3D cell culture preparation. RAFT 3D cell cultures main
Cell Culture Methods for in vitro Toxicology introduces the reader to a range of techniques involved in the use of in vitro cell culture in toxicological studies. It deals with major cell types studie
483575805 - EP 2980202 B1 2017-10-11 - CELL CULTURE APPARATUS AND CELL CULTURE METHOD - [origin: EP2980202A1] An apparatus for culturing cells of the present invention is an apparatus for culturing cells using a culture medium in a culture vessel. The apparatus includes: pump (4) connected to pipet (2) held by first holder (3); first shifter (5) configured to shift first holder (3); second holder (11) configured to hold culture vessel (10); second shifter (16) configured to grip and shift culture vessel (10); and controller (15). Controller (15) is programmed to, when pipet (2) draws a liquid in culture vessel (10) or discharges a liquid into culture vessel (10), control second shifter (16) to translationally shift lid (10a) of culture vessel (10) in a horizontal direction.[origin: EP2980202A1] An apparatus for culturing cells of the present invention is an apparatus for culturing cells using a culture medium in a culture vessel. The apparatus includes: pump (4) connected to pipet (2) held by first
SMi Group proudly presents the inaugural launch of 3D Cell Culture, an exciting new conference scheduled to take place on the 22nd and 23rd February 2017, London UK. Following the success of our Advances in Cell Based Assays series, 3D Cell Culture is set to become a leading event for industry. 3D Cell Culture 2017 will address the latest developments of 3D cell culture techniques; the ways in which predictive 3D models are presently paving the way to future technologies, and the ways in which they are currently revolutionizing drug discovery and screening and disease modelling. The 3D cell culture market is predicted to reach $3702.2 million by 2021* with main increase seen in novel technologies and culture methods. This event will highlight emerging technologies, like 3D and 4D bio imaging, and their application in furthering research and medical practice. In addition, there will be focus on organoid technology and the involvement of 3D culture methods in drug and toxicology screening, topics ...
Whether youre just getting started in 3D cell culture, looking for proven ways to scale up, or moving to high throughput screening, Corning can help you break through the barriers to creating more in vivo-like environments and predictive models. Quickly and efficiently.. For more than 25 years, Corning has delivered innovations that have advanced the science of 3D cell culture. We pioneered the development of novel tools providing easier access to in vivo-like 3D models, such as Corning Matrigel® matrix and Transwell® permeable supports. And we continue to support you with a diverse and evolving portfolio of innovative 3D cell culture products, like the Corning spheroid microplate, as well as workflow solutions, protocols, and expertise ...
Browse 66 market data Tables and 72 Figures spread through 158 Pages and in-depth TOC on "3D Cell Culture Market by Technology (Scaffold Based, Scaffold Free) by Application (Cancer Research, Stem Cell Technology, Primary Cell Research, Tissue Engineering & Regenerative Medicines, Human Cell Lines, Tumor Xenografting, Drug Discovery, and Others) by End-Users (Research Laboratories, Biotechnology and Pharmaceuticals Industries, Hospitals and Diagnostics Centers and Others) - Global Forecast to 2021". http://www.marketsandmarkets.com/Market-Reports/3d-cell-culture-market-191072847.html. Early buyers will receive 10% customization on reports.. The global 3D cell culture market size is expected to reach USD 1345.2 Million by 2021, at a CAGR of 23.6% during the forecast period of 2016 to 2021.. The global 3D cell culture market is steadily progressing owing to various factors, such as increasing research in 3D cell culture, investments done by pharmaceutical and biotechnology companies in 3D cell ...
We describe a chip-based platform for the three-dimensional cultivation of cells in micro-bioreactors. One chip can house up to 10 Mio. ...
PromoCell offers a range of kits 3D cell culture applications including different 3D Matrix Kits as well as the Individual Matrices (e.g. BME/Matrigel & Alginate-Hydrogel), a high-throughput-compatible 3D Scaffold Kit in a ready-to-use 96-well format, a 3D Cell Harvesting Kit as well as a 3D Cell Viability Kit. These kits include all required components and a comprehensive, simple protocol for setting up a 3D cell culture, to recover cells from different matrices and to determine cell viability/proliferation & cytotoxicity in 3D cell culture.. ...
We have demonstrated here that CTL007 migrate through a 500 μm collagen/fibroblast separating layer toward tumor cells, resulting in tumor cell apoptosis. We have also shown that migration is dependent on CCR2 expressed by T cells and CCL2 secreted by tumor cells.. Our recently developed novel three-dimensional culture system offers a unique way of studying migration of leukocytes toward tumor cells and the factors that influence leukocyte migration under physiological conditions [32, 34]. As described in our previous studies, human CRC is grown in vitro under three-dimensional conditions using a mixture of collagen and fibroblasts [32, 44]. Interaction of α2 and β1 integrins on CRC-specific T cells with collagen and the presence of activated fibroblasts help to maintain Ag-specific T cells in a state of activation in absence of exogenous addition of IL-2 [36, 37]. In addition, T cells could interact with fibroblasts via adhesion molecules like LFA-1a, ICAM-1 and CD44 which could in turn ...
With the adoption of 3D methods and complex co-culturing becoming a growing trend, cell culture media optimization has also become a challenging factor for researchers. Lonza helps researchers ease this transition with BulletKit™ Growth Media, which are robust in supporting co-culture studies. Depending on the specific needs of the co-culture, Lonzas scientific support team can offer proven solutions supported by a long history of published literature. For cancer research in particular, where both cell lines and primary cells are equally significant, BulletKit™ Media provides researchers with added flexibility and reduced variability across their experiments as the same media can be used to support the growth of both cell types. This also simplifies experimental design. Take for instance, MEGM™ Mammary Growth BulletKit™, which has been utilized by researchers to support growth of Lonzas primary mammary epithelial cells and well-established breast cell lines, MCF-10A and MCF-12. For ...
The epithelial-mesenchymal transition allows cancer cells to remodel the extracellular matrix and invade tissues. However, invasive squamous cell carcinoma (SCC) cells do not lose their epithelial markers, which led Gaggioli et al. to investigate how these cells invaded tissues. The authors devised a three-dimensional culture system consisting of a matrix block, containing mainly collagen and laminin, placed in culture medium with its upper surface exposed. SCC cells placed on this surface did not invade the matrix when cultured alone but did so when cultured with fibroblasts from tumors, as determined by immunohistochemical staining. Invasion by SCC cells was blocked when a thin sheet of matrix was placed between the fibroblasts and the SCC cells. Differential fluorescent labeling of SCC cells and fibroblasts showed that a chain of invading SCC cells in the matrix was always preceded by a fibroblast. Electron and reflectance microscopy revealed that "tracks" in the matrix generated by ...
This section provides useful hints for culturing animal cells (i.e., cells derived from higher eukaryotes such as mammals, birds, and insects). It covers different types of animal cell cultures, considerations for cell culture, and cell culture protocols.
H. (1986) Use of the virus to prepare human-derived monoclonal antibodies, in The Epstein-BarrVirus: RecentAdz~ances (Epstein, M. A. and Achong, B. ), William Heinemann Medical Books, London, p. 249. Adams, A. (1975) EBV Production, Concentration and Purification (Ablushi, D. , Aalesed, H. ), IARC, Lyon, France, p. 129. Miller, G. and Lipman, M. (1973) Release of infectious Epstein-Barr virus by transformed marmoset leucocytes. Nutl. Acud. USA 70,190. Chapter 6 Scale-Up of Suspension and Anchorage-Dependent Animal Cells J, Bryan Griffiths 1. 5. Check for proliferation after 7 d. 6. Remove medium and cells from wells, spin down, and gently resuspend transformed cells in fresh medium. Continue to culture in 24well plates. 7. These cells can be grown inlimiting dilution for B-cell characterization with monoclonal or polyclonal antibodies or functional assays. 8. Maintenance Using B-Cell Growth of B-CeZZs Factor Recently, B cells from peripheral blood from normal donors grown in the presence of ...
The majority of in vivo models of metastasis determine the effect of experimental conditions (usually via knock-out mice) by relying on the end point of secondary tumor formation at a distal site (14). However, the steps occurring between tumor cell injection and metastatic tumor formation are effectively shrouded in these models (15). Despite advances in imaging to monitor these processes, a crucial step of metastasis-the invasion and colonization of cancer cells upon extravasation-remains an elusive target. This study describes a three-dimensional culture system developed to model this phase in vitro and establishes the efficacy of this novel system by demonstrating that cells from disparate phases of melanoma invade the matrix in a manner proportional to their known metastatic capabilities. Specifically, the highly metastatic C8161.9 stayed true to its documented in vivo behavior (21), ferociously staking claim to much of the surrounding matrix (Fig. 1A-C). The sporadically metastatic M14 ...
Researchers in Austria have developed a new three-dimensional culture system for growing mini brains in the lab. They began with ...
The latest news and articles on cell culture protocols, techniques, methods and processes from the leader in serving science - Accelerating Science by Thermo Fisher.
We characterized fully and adapted a method15 to isolate ECs from mouse aortas that allowed us to profile gene expression in ECs during the early stages of atherosclerosis. This method provides a quick and efficient way to collect MAEC RNA, generally yielding RNA from a mouse in ,1 hour after harvesting, and has several advantages instead of cell culture methods. Not only do we avoid the passage and dedifferentiation issues that occur during cell culture procedures but we are able to directly assay the transcriptional state of the cells that closely represents their in vivo status. In these experiments, we were able to obtain the transcriptional state of ECs in prelesion aortas, and through the use of 1 RNA amplification, we were able to perform microarray analysis on MAECs taken from a single mouse. Moreover, we demonstrate that the whole aorta, with its endothelium intact, can be treated in cell culture medium similar to conventional cell culture with the added advantage of not disrupting the ...
3D Cell Culture is much better at replicating in vivo environment than traditional two-dimensional cultures. Learn about the history of spheroids.
A suspension cell culture system where a culture chamber is rotatable about a horizontal axis and has a vertical large area oxygen transmissible membrane spaced a distance about 0.25 inches less than 1.0 inches from a facing vertical wall surface for effective transmission of oxygen to cells in suspension in the culture chamber. The facing vertical wall surface can be a dialysis membrane for exchange of fresh nutrient from a dialysis chamber with cell waste product in the culture chamber.
3D cell culture technique triggers normal cell morphology, proliferation, differentiation, and migration. It provides an ideal environment for cell culture
Scientists describe the optimization of the fabrication process for a novel, detailed, 3D cell culture platform based on direct laser written tubular microtowers and human neuronal cells.
... 18th KCLB Workshop. Basic Cell Culture Techniques Hands-on Workshop. 국가목적형 소재은행인 한국세포주은행에서 아래와 같이 18차 KCLB Workshop, Basic Cell Culture Techniques Hands-on Workshop을 개최합니다. 참가자는 10명내외의 인원으로 구성하여 하루동안 이론교육 및 실습이 진행됩니다. 접수는 11월 23일까지이며, 참가여부는 개별통지하며, 무료교육입니다.. 관심있으신 연구자께서는 한국세포주은행 홈페이지(서비스-,Hands-on Workshop)에서 신청하여 주시고, 문의사항은 전화(02.3668-7915), 이메일([email protected])로 문의하여 주시기 바랍니다.. 감사합니다.. ...
Thawing - should occur quickly and cells diluted in pre-warmed culture medium to prevent any toxic effects of cryoprotectants in super-zero temperatures. Incubate and examine (phase contrast) the next day. Cells may need to be washed in media if cryoprotectant has a known adverse cytopathic effect ...
Mammalian cell culture has long been an invaluable tool in cell biology, drug discovery, and regenerative medicine. When cell culture techniques were first developed, 3-Dimensional (3D) systems were utilized. That rapidly changed due to cost and efficiency concerns resulting in cell culture being performed today with adherent cells grown on flat and rigid two-dimensional (2D) substrates, including polystyrene or glass. Advances in our understanding of cell physiology and failures in clinical trials have provided the impetus to move away from 2D systems and back to a more in-vivo-like 3D environment. The advance of these new technologies and screening methodologies have allowed scientists to assess more realistic functional capabilities of cells. The following course will focus on the cellular microenvironment and its importance when developing and screening cell-based assays using primary, stem cell, and immortalized cultures in 3D systems.. ...
The Global 3D Cell Culture Scaffold Market 2017 Industry Research Report is a professional and in-depth study. This study covers, 3D Cell Culture Scaffold
3D Cell Culture Market Report offered by Market Study Report gives a market overview of the 3D Cell Culture industry which covers product scope, market revenue, opportunities, growth rate, sales volumes and figures. The report also explores the worldwide players of the market and is segmented by region, type and application with forecast to 2022.
The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. The invention is based, at least in part, on the discovery that cycling the cultures in this manner optimizes the formation of extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue.
Basel, Switzerland, 12 February 2018 - Today Lonza announced the latest addition to its cell-culture product portfolio - the Quasi Vivo® System. The Quasi Vivo® Device consists of an advanced, interconnected fluidics system to create more physiologically relevant cell-culture conditions, helping researchers improve the predictive value of their studies. This new product offering from Lonza is a result of a worldwide marketing and distribution agreement with Kirkstall - a biotechnology company based in Rotherham (UK).. A common issue faced by drug discovery scientists who use conventional in vitro culture systems is their poor translatability to humans. To address this problem, Kirkstall developed the Quasi Vivo® System, which consists of interconnected cell-culture chambers and a peristaltic pump to create a continuous flow of media over cells. As a result, cultures are exposed to more physiologically relevant conditions, increasing the predictive value of in vitro experiments.. The Quasi ...
WHEATON Industries has acquired the CELLine Bioreactor Flask Line for Antibody and Protein Production from Wilson Wolf Manufacturing Corporation. The flasks enhance small scale bio-production for antibody and protein generation. Conventional in vivo or in vitro cell culture methods can be laborious, result in low cell density, and require significant purification.
Fascinating biology occurs at epithelial interfaces, whether between organism and environment or within body compartments, and many diseases inflicting huge personal and societal burdens result from d
A polypeptide having the following formula is provided: gly-glu-phe-tyr-phe-asp-leu-arg-leu-lys-gly-asp-lys which can bind heparin and promote cellular adhesion. Medical devices such as prosthetic implants, percutaneous devices and cell culture substrates coated with a composition including the polypeptide are also provided.
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A polypeptide which can bind heparin and promote cellular adhesion and neurite outgrowth is provided of the formula: lys-asn-asn-gln-lys-ser-glu-pro-leu-ile-gly-arg-lys-lys-thr, leu-ile-gly-arg-lys-lys-thr, tyr-arg-val-arg-val-thr-pro-lys-glu-lys-thr-gly-pro-met-lys-glu, ser-pro-pro-arg-arg-ala-arg-val-thr, trp-gly-pro-pro-arg-ala-arg-ile, or mixtures thereof. Medical devices such as prosthetic implants, percutaneous devices and cell culture substrates coated with the polypeptide composition are also provided.
Our mission is to be the unrivaled leader of 3D cell culture technology by innovating and exploring the potential of the rotary cell culture system
This article provides a comprehensive overview with detailed references of the techniques employed in 3D cell culture, demonstrating how 3D systems provide a more relevant readout over their 2D assays counterpart.. Please complete the short form to download this helpful article.. ...
Buy Alvetex Perfusion Plate Kit (Perfusion plate x 2 and Alvetex Scaffold 12 well insert x 12) by Alvetex from our 3D Cell Culture range - Alvetex kits - @ REPROCELL Global Products & Services for Drug Discovery & Regenerative Medicine
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Institute of Bioinformatics (IOB) is a premier research center for Genomics, Proteomics, Metabolomics, Human cell culture and Computational biology. IOB has established a state-of-the-art human cell culture facility that are being utilized to explore and validate candidate biomarkers in a wide array of human diseases ranging from neurological disorders and infections to cancers. Scientists at IOB will share their expertise in these cell culture techniques with young researchers across India through a series of workshops. Several faculties and students from reputed Research Institutes and Universities from India have participated in these workshops. In the year 2016 we have initiated a new series of workshops which will benefit students and researcher in the field of biomedical research ...
한국세포주은행은 12년간 세포주연구워크샵을 진행하여 183개의 주제, 3,850명의 국내 연구자에게 최신 세포주연구기법을 소개해왔습니다. 많은 부분 국내 세포주관련 연구가 잘 정착이 되었기에 2013년부터는 소수의 연구자 대상으로 실질적 세포주 배양에 관련된 부분(Basic cell culture techniques) 과 많은 연구자들께서 관심을 보이시는 조직으로부터 세포주를 수립하는 부분(Primary cell culture), 그리고 수립된 세포주를 품질관리하는 부분(Quality control of cell lines)으로 나누어 워크샵을 진행하고자 합니다.. 관심있으신 연구자께서는 메일이나 전화를 주시면, 일정 확정시 참여하실 수 있도록 연락을 드리겠습니다. 이에 관련하여 문의사항이 있으신 분들은 한국세포주은행으로 직접 전화(02.3668-7915)나 이메일([email protected])로 문의하여 주시기 바랍니다. 감사합니다.. ...
One of Dr. Griffiths most actively cited publication, "Capturing Complex 3D Tissue Physiology in vitro," provides guidelines and design principles when determining how to recreate tissue microenvironments using 3D tissue models. 3D tissue models allow researchers to study tissue physiology and pathology in vitro. Some of the salient factors for the adoption of 3D models include the use of human cells over animal cells, pre-clinical drug screening, and superior complexity compared to 2D culture models. Dr. Griffith the third dimension may prove critical in the study of tissue models because of the changed effects of mechanical input and cell adhesion, thereby impacting cell to cell signaling and cell contraction. Three dimensional tissue also creates a potential to observe cell morphology events only possible over large distance scales. Dr. Griffin also cites the importance of matrix stiffness in impacting cell differentiation. One experiment on breast-epithelial-cell culture showed that cells ...
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Gudjonsson immortalised these cells and grew them in three dimensional matrix that mimics the real, living tissue. Biologists have long relied on 2-dimensional cell cultures as the basic tool of their trade. But there is a big difference between a flat layer of cells and culturing cells in three-dimensions. The Icelandic researchers, realizing just how much a cells context matters, used the 3-D cell culture pioneered by Mina Bissell, at the Lawrence Berkeley National Laboratory in California. "We can build up a 3-D breast structure similar to what you have in vivo," says Gudjonsson ...
... - Instruments Consumables Reagents Advanced BioMatrix,RANDOX,RANDOX ELISA,Biomedical, biochemical reagents, laboratory supplies, equipment, antibodies, ELISA kits, diagnostic reagents, methods of experimental techniques, general analytical instruments, material testing instruments and equipment, used laboratory equipment, instruments and equipment, life sciences, environmental monitoring equipment , measurement, measuring instruments, rotating wall bioreactor, three-dimensional tissue / stem cell culture system; microcapsule
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Pancreatic cancer is one of the most deadly forms of cancer, with overall 5-year survival rates under 20%. The only cure for this type of cancer is surgical, however this option is rarely available due in part to detection that is typically late in the progression of the disease. The aim of this study was to understand the differences in progression between L3.6, PANC-1, and M1A-PaCa-2 cells as representatives of varied levels of metastatic character through aggregation analysis in 3D cell culture. It is well established that 3D culture more closely mimics the in vivo condition, as the cells adhere to each other forming "microtumors" as opposed to being attached to a plastic substratum as is the case with standard 2D cell culture. We also examined the effectiveness of a novel target for chemotherapeutic intervention in these cell lines in 3D culture. The sigma-2 receptor is highly upregulated in rapidly proliferating cancer tissues as compared to non-cancerous tissue, and induces apoptosis when ...
In recent years, huge steps have been made to standardize and manufacture equipment for 3D cell culture-making it cheaper, simpler and more reproducible. As the materials for 3D methods become more accessible, easier to use and less expensive it is likely that the 3D-approach will become more and more popular in both basic biological research and drug-assay trials
Possibilities for Basic Research, Drug Discovery, and Therapeutics Expand Geometrically http://www.genengnews.com/gen-articles/watch-this-3d-cell-culture-space/6089
Conference on 3D cell cultures as advanced model systems for understanding of diseases, compound testing, successful translation from models to clinical and industrial solutions, and on enabling technologies. 5-7 June 2018, Konzerthaus Freiburg, Germany.
Daily News How Gaining and Losing Weight Affects the Body Millions of measurements from 23 people who consumed extra calories every day for a month reveal changes in proteins, metabolites, and gut microbiota that accompany shifts in body mass.. ...
According to a report issued by the Tufts Center for the Study of Drug Development, the costs associated with bringing a novel drug to market exceed $2.5 billion per successful compound
Integrated approaches using different in vitro methods in combination with bioinformatics can (i) increase the success rate and speed of drug development; (ii) improve the accuracy of toxicological risk assessment; and (iii) increase our understanding of disease. Three-dimensional (3D) cell culture models are important building blocks of this strategy which has emerged during the last years. The majority of these models are organotypic, i.e., they aim to reproduce major functions of an organ or organ system. This implies in many cases that more than one cell type forms the 3D structure, and often matrix elements play an important role. This review summarizes the state of the art concerning commonalities of the different models. For instance, the theory of mass transport/metabolite exchange in 3D systems and the special analytical requirements for test endpoints in organotypic cultures are discussed in detail. In the next part, 3D model systems for selected organs liver, lung, skin, brain are ...
This may seem very simple, but I cant figure it out. Im using wglUseFontBitmaps to draw 2D text in a 2D overlay, kinda like a HUD in a 3D game. Now I want to use the 2D text in the 3D environment. An object with a 3D coordinate should have a flat text with info about it straight above it. Right now I use glRasterPos2f to position the text. Ive messed with glRasterPos3f, gluPerspective, glOrtho, switched the matrix mode between GL_PROJECTION and GL_MODELVIEW, etc; either it shows nothing
Cell culture dishes / petri dishes: We focused on improved handling and stacking performance of the cell culture dishes to ensure a new level of safe
... publishes research articles on current and future clinical applications of noninvasive and invasive imaging techniques including echocardiography, CT, CMR, nuclear, angiography, and other novel techniques.. ...
The findings of this study support the feasibility of using the nasal methylome for future clinical applications, such as predicting the development of asthma among wheezing infants.. ...
Revision as of 05:21, 9 November 2015 by Wev012000 (Talk , contribs) (Created page with {, class=wikitable sortable collapsible ,- ! ID ! Icon ! Cell Culture Dish ! D N A Sampler ! Release Version ,- , 1fC , Image:Cell_culture_dish.png,link=Cell Culture Dish...) ...
TeSR™2 is a high-quality, robust system for feeder-independent maintenance of human ES & iPS cells in a defined & humanized culture environment.
Why Optimize? The proper composition of the ECM, its presentation to the cell, and its appropriate stiffness significantly improve the biological relevance of an in vitro cell culture since the cell acts and looks far more like its in vivo counterpart in the presence of the appropriate soluble medium.
There are many antibiotics available and it is important to know when and how to use them. This session is a good opportunity to ask your questions and get answers.
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Extracorporeal formation of mineralized bone-like tissue is still an unsolved challenge in tissue engineering. Embryonic stem cells may open up new therapeutic options for the future and should be an interesting model for the analysis of fetal organogenesis. Here we describe a technique for culturing embryonic stem cells (ESCs) in the absence of artificial scaffolds which generated mineralized miromasses. Embryonic stem cells were harvested and osteogenic differentiation was stimulated by the addition of dexamethasone, ascorbic acid, and ß-glycerolphosphate (DAG). After three days of cultivation microspheres were formed. These spherical three-dimensional cell units showed a peripheral zone consisting of densely packed cell layers surrounded by minerals that were embedded in the extracellular matrix. Alizarine red staining confirmed evidence of mineralization after 10 days of DAG stimulation in the stimulated but not in the control group. Transmission electron microscopy demonstrated scorching
VitroGel 3D-Advanced hydrogel for 3D cell culture and spheroid culture research. We offer many matrices and other solutions for 3D cell and spheroid culture
Tissue culture has been used for over 100 years to study cells and responses ex vivo. The convention of this technique is the growth of anchorage dependent cells on the 2-dimensional surface of tissue culture plastic. More recently, there is a growing body of data demonstrating more in vivo-like behaviors of cells grown in 3-dimensional culture systems. This manuscript describes in detail the set-up and operation of a hollow fiber bioreactor system for the in vivo-like culture of mammalian cells. The hollow fiber bioreactor system delivers media to the cells in a manner akin to the delivery of blood through the capillary networks in vivo. The system is designed to fit onto the shelf of a standard CO2 incubator and is simple enough to be set-up by any competent cell biologist with a good understanding of aseptic technique. The systems utility is demonstrated by culturing the hepatocarcinoma cell line HepG2/C3A for 7 days. Further to this and in line with other published reports on the ...
Human cancer cell lines are an integral part of drug discovery practices. However, modeling the complexity of cancer utilizing these cell lines on standard plastic substrata, does not accurately represent the tumor microenvironment. Research into developing advanced tumor cell culture models in a three-dimensional (3D) architecture that more prescisely characterizes the disease state have been undertaken by a number of laboratories around the world. These 3D cell culture models are particularly beneficial for investigating mechanistic processes and drug resistance in tumor cells. In addition, a range of molecular mechanisms deconstructed by studying cancer cells in 3D models suggest that tumor cells cultured in two-dimensional monolayer conditions do not respond to cancer therapeutics/compounds in a similar manner. Recent studies have demonstrated the potential of utilizing 3D cell culture models in drug discovery programs; however, it is evident that further research is required for the development of
Many types of mammalian cells can aggregate and differentiate into 3-D multicellular spheroids when cultured in suspension or a nonadhesive environment. Compared to conventional monolayer cultures, multicellular spheroids resemble real tissues better in terms of structural and functional properties. Multicellular spheroids formed by transformed cells are widely used as avascular tumor models for metastasis and invasion research and for therapeutic screening. Many primary or progenitor cells on the other hand, show significantly enhanced viability and functional performance when grown as spheroids. Multicellular spheroids in this aspect are ideal building units for tissue reconstruction. Here we review the current understanding of multicellular spheroid formation mechanisms, their biomedical applications, and recent advances in spheroid culture, manipulation, and analysis techniques. ...
More people die annually from cardiovascular diseases than from any other cause. In particular, patients who suffer from myocardial infarction may be affected by ongoing adverse remodeling processes of the heart that may ultimately lead to heart failure. The introduction of stem and progenitor cell-based applications has raised substantial hope for reversing these processes and inducing cardiac regeneration. However, current stem cell therapies using single-cell suspensions have failed to demonstrate long-lasting efficacy due to the overall low retention rate after cell delivery to the myocardium. To overcome this obstacle, the concept of 3D cell culture techniques has been proposed to enhance therapeutic efficacy and cell engraftment based on the simulation of an in vivo-like microenvironment. Of great interest is the use of so-called microtissues or spheroids, which have evolved from their traditional role as in vitro models to their novel role as therapeutic agents. This review will provide ...
ExCellness Biotech SA was incorporated in 2009 as a spin-off project from the Ecole Polytechnique Fédérale de Lausanne (EPFL) in Switzerland.
Mature human β-cells are typically considered as terminally differentiated cells that do not switch their hormone production once fully differentiated. Our results show that human β-cells can convert rapidly and preferentially into glucagon-producing α-cells without forced expression of exogenous transcription factors. Of importance, the conversion occurred in islets from pancreas of organ donors from different backgrounds, independent of donor factors such as age or sex.. To date, only a few examples of human cell transdifferentiation have been reported. This usually requires the forced expression of transcription factors or miRNAs to convert human fibroblasts into neurons, hematopoietic progenitors, or brown fat cells (21) or human liver cells into β-cells (22). In our three-dimensional culture system, the dispersion into single cells followed by reaggregation into islet cell aggregates is likely to be a critical step for cell conversion. Previous lineage-tracing studies in human β-cells ...
Here researchers employ three-dimensional culture systems for conditional gene targeted primary mouse embryonic fibroblasts that better simulate the reciprocal and adaptive interactions between cells and surrounding matrix, to define the role of Cdc42 signaling pathways in extracellular matrix organization. [J Biol Chem] Abstract ...
The functional interactions that define the bilayered acinus have been explored using three-dimensional culture systems. When phenotypically normal human or rodent luminal cells are grown in laminin-rich extracellular matrix (lrECM) gels, they recreate the structure and function of the acinus found in vivo even in the absence of myoepithelial cells [6, 18]. We believe that this is possible, in part, because cultured luminal cells express a number of proteins that are characteristic of myoepithelial cells in vivo (e.g. β4 integrin [10], epidermal growth factor receptor [19], vimentin [20], maspin [21], and others; for review [1]). It may be that luminal cells can form acinar structures in culture because of this ability to become luminal/myoepithelial hybrids. The possibility that expression of specific myoepithelial proteins confers distinctive signaling cues that promote cell survival and proper apicobasal polarity is an active area of investigation in our laboratory and those of our ...
Abstract While most of the in vitro cultures are carried out on bi-dimensional (2D) substrates, most of the in vivo extracellular matrices are threedimensional (3D). Consequently cells behave differently on 2D substrates as a way to self-adaptation to a non-physiological environment. This fact has encouraged the development of more relevant culture conditions seeking to provide more representative models for biomedicine (e.g. cancer, drug discovery and tissue engineering) and further insights into any dimension-dependent biological mechanism. Different 3D culture systems have been established though their variability and complexity hinder their standardisation in common cell culture procedures. So, this thesis deals with the dimensionality issue in cell/material interactions and introduces sandwich-like microenvironments as a versatile tool to study cell behaviour. Cells cultured within this system use both dorsal and ventral receptors to adhere and spread, undergoing important changes with ...
This workshop is designed for those new to mammalian cell culture or needing a refresher course on the basics. The workshop includes informal lectures and hands-on experience. The major topics are: maintaining mammalian cells in culture (aseptic technique, thawing, counting, expanding, freezing) and troubleshooting (rescuing sick cells and recognizing and dealing with contamination).. ...
cell passage number - posted in Tissue and Cell Culture: Hi. I am wondering if someone could explain to me about the relationship between Vero cells passage number and antiviral activity. I mean whether it true that after a number of passages, the antiviral activity of Vero cells (or cells in general) might fall or display nothing at all. any suggestion or recommendation is appreciative. Cheers.
3D Cell Culture Webinar: View Lonzas archived webinar and learn how you can enhance your cell culture using RAFT™ 3D Cell Culture System. Watch it now!
Determine maximal passage number - posted in Tissue and Cell Culture: Hey guys, I am doing some cell culture work right now and we have different cell lines (human pericytes, astrocytes and endothelial cells). My supervisor told me I should not culture them more then passage number 10 or 11. How would you determine the maximal passage number for a specific cell line? Thank you for your help
The two key issues in chemo- and radiation therapy is the development of tumor resistance as well as toxic effects on normal tissue. In this sense new strategies are required to increase efficacy of radiation to improve the therapeutic impact and reduce toxicological side effects. The performance of 3D cell culture systems over classical 2D culture systems has been shown to provide a closer representation of tissue-level biology. This has led to the rapid adoption of 3D systems for both drug discovery and toxicology. InSphero has developed a highly reproducible hanging drop technology able to generate monotypic cell spheroids (microtissues) in a 96-well format. The innovative 3D-microtissue plate technology has been adapted for analysis of the cellular response of radioresistant T47D breast cancer cells to combined radio-chemotherapy (RCTx). We have validated the model by comparing the treatment of microtissues with 10 different chemotherapeutic compounds, each tested alone and in combination ...
We have previously shown that BMP4 reduces proliferation and increases migration of breast cancer cells in vitro [10]. As these results were derived from cells grown in 2D monolayer culture, we set out to analyze the effect of BMP4 in a more physiological setting by employing 3D culture systems. We approached this issue by using both a biological gel (Matrigel, the standard 3D culture environment) and a synthetic material with RGD peptides and MMP-degradable peptide links (PEG gel).. The two materials studied provided dissimilar 3D environments as first evidenced by differences in the morphology of the normal and cancer cell clusters. The MCF-10A normal mammary epithelial cells had a polarized acini structure in Matrigel, as previously shown [17], while in PEG gel the cells formed irregular non-polarized structures. Similarly, the morphology of the different cancer cells varied between the two 3D models, with the structures formed in Matrigel again corresponding to those previously reported ...
We routinely freeze cells in 10%DMSO - 90%FCS, and have NOT been successful in freezing hybridoma cells that have been adapted to serum free conditions. We have recently embarked on further efforts to do this by investigating alternative freezing media. I have seen some articles (I forget where) suggesting use of glycerol or glycerol/DMSO combinations as cryoprotectants for cell lines that are otherwise difficult to freeze. We have tried to freeze 3 or 4 different serum-free adapted hybrids, all with the same crummy results. Good luck, hope your luck is better than ours. Fred Garbrecht Medical College of Wisconsin fgarbrec at post.its.mcw.edu On 3 Jun 1993, AMRUT S BHOGLE wrote: , Date: 3 Jun 93 17:12:19 GMT , From: AMRUT S BHOGLE ,AMRUT at UCSVAX.UCS.UMASS.EDU, , To: methods-and-reagents at net.bio.net , Subject: serum replacements , , Dear Readers, , , I have been trying to adapt a chicken lymphoblastoid cell-line , to serum-free conditions. I have been trying to freeze cells for posterity , ...
The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. The invention is based, at least in part, on the discovery that cycling the cultures in this manner optimizes the formation of extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue.
The factors and signaling pathways controlling pluripotent human cell properties, both embryonic and induced, have not been fully investigated . Failure to account for functional heterogeneity within
By definition, a morphogen is an instructive molecule that can specify two or more cell fates in a concentration-dependent manner (Ashe and Briscoe, 2006). In metazoans, morphogens often share other features including secretion from localized signaling centers, resulting in concentration gradients within target tissues, and the ability to act directly on cells at both short- and long-ranges (Grove and Monuki, 2013; Kicheva and Gonzalez-Gaitan, 2008; Tabata and Takei, 2004). Many such morphogens are now well known in invertebrate systems (Kicheva et al., 2007; Porcher and Dostatni, 2010). In vertebrate CNS model systems, classic morphogens are also thought to exist, including Sonic hedgehog (SHH) in the spinal cord, retinoic acid (RA) in the hindbrain, and fibroblast growth factors (FGFs) in the rostral-most telencephalon (Dessaud et al., 2007; Ericson et al., 1997; Schilling et al., 2012; Stamataki et al., 2005; Toyoda et al., 2010). These examples have relied largely on in vivo models, in which ...
Read an interview with Dr. Madeline Lancaster and learn more about Brain Organoids, Cerebral Organoids or Mini-Brains: ESC and iPSC derived 3D culture systems that mimic human brain development in a dish and can be used to model neurological diseases.
Since the adult myocardium lacks the inherent ability to repair itself following ischemic injury, exogenous cell sources for the repair of infarcted myocardium have been investigated, and it has been demonstrated that transplanted cells may impart functional benefits via the secretion of growth factors and extracellular matrix (ECM) molecules.  Such mitigation of tissue repair by cell-derived molecules represents a significant paradigm shift in the therapeutic application of stem and progenitor cells and provides a novel approach to myocardial repair.  My research aims are to (1) examine the effects of three-dimensional cell culture systems on adult progenitor cell differentiation, growth factor synthesis, and ECM production, and (2) to determine the effects of progenitor cell-derived factors on ischemic cells and tissues in vitro and in vivo.  These studies should yield new insights into the cues presented to injured myocardium by exogenously administered ...
We offer a broad portfolio of three-dimensional cell culture systems including MaxGel human Extracellular Matrix (ECM), HydroMatrix synthetic peptide, and mouse ECM, to support stem cell and other cell cultures.
Our laboratory has had long-standing thematic interests in the cell-type and tissue-type specific actions of certain oncogenes (cyclin D1, EGFR) and tumor suppressor genes (p53, p120 catenin) in modulating the initiation, progression and invasion of gastrointestinal cancers, especially upper GI, pancreatic and colon. To that end, we employ novel three-dimensional cell culture systems (mouse and human origins) and genetically-engineered mouse models to investigate molecular mechanisms. These projects are translated into the objectives of improving molecular diagnostics and experimental therapeutics in patients ...
The Stem Cell Culture Systems RCCS-2SC includes a dual vessel rotator base, operation manual, two stem cell vessels of your choice and a one year warranty.
With Cellartis clinical-grade hES cell line derivation services, we will deliver clinical-grade human ES cell lines for use in clinical research settings. The proprietary hES cell establishment method is feeder-free and animal/human-component free. The starting material is retrieved from FDA-compliant sources according to FDA guidelines. For more information, visit the services overview page and fill out the inquiry form on the right-hand side.. ...
Gottwald reports that the real motivation for establishing a company was to harvest the fruits of their work that had led to the development of a marketable product. And he adds: "Being able to do this in ones own company is great fun. It is a whole different world and a fantastic challenge. You get to know completely different people, gain new skills and be much closer to real life. You see the proverbial ivory tower of science through completely different eyes, and realize that it is actually a tower.". After the establishment of 300MICRONS, Gottwald reduced his research commitments at the KIT: in the morning, he works at the KIT where he heads up the 3D Cell Culture group, and he then spends his afternoons in the company. "This works excellently," Gottwald says. "And the reason it works so well is thanks to the KIT management who gave us every imaginable kind of support, including allowing Gottwald and his colleagues to work flexible hours. The KIT is always interested in spin-offs as it is ...
At this years Boston Biotech Week, Kristin ONeill and Ankit Mehta of Merck gave a very interesting talk titled, "Experiences automating the Roche Cedex Bio HT and HiRes with the Flownamics SegFlow on-line sampling system." In the talk, they detailed their process and ultimate success in creating an automated system for cell density, cell viability, and cell culture medium analysis of CHO cell bioreactor cultures. The system consists of 3 components: the Seg-Flow 4800 (Flownamics) automated sampler and the Cedex HiRes and Bio HT analyzers (Roche Custom Biotech). The project was a beta site study developed as a collaborative effort of the three companies. In beginning this project, Merck wanted to improve operational efficiency by looking closely at their in-process analytics. The initial goal described in this presentation was to provide complete, automated analytics for a dual-feed bioreactor culture system. As a ready-to-use solution was not available, Merck partnered with Roche Custom ...
Potential of this technology to replace and reduce the usage of animal models for histological analysis and biochemical assays is expected to fuel demand for 3D Cell Culture products thus driving growth in the coming years. Advent of technology with respect to spheroid formation and matured assay methods, is expected to boost the emergence of…
13. A method for preparing a cell culture substrate comprising: a composite (X) having a three-dimensional network formed of a polymer (A) of a monomer comprising a monomer (a) represented by Formula (1) below and at least one inorganic material (C) selected from a water-swellable clay mineral and silica: ##STR00008## wherein R1 is a hydrogen atom or a methyl group, R2 is a C2-C3 alkylene group, R3 is a C1-C2 alkyl group and n is an integer of 1 to 9; and a polymer (B) having a lower critical solution temperature: the method comprising: a first step of mixing the monomer (a), the inorganic material (C) and a polymerization initiator (D) in an aqueous medium (W) such that the concentration of the inorganic material (C) in the aqueous medium (W) is within the range represented by the following Formula (2) or (3), and polymerizing the monomer (a) to provide a dispersion (L) of the composite (X) comprising the polymer (A) and the inorganic material (C); a second step of applying the dispersion (L) ...
Electrospinning is a versatile method to fabricate nanofibers of a range of polymeric and composite materials suitable as scaffolds for tissue engineering applications. In this study, we report the fabrication and characterization of polyaniline-carbon nanotube/poly(N-isopropyl acrylamide-co-methacrylic acid) (PANI-CNT/PNIPAm-co-MAA) composite nanofibers and PNIPAm-co-MAA nanofibers suitable as a three-dimensional (3D) conducting smart tissue scaffold using electrospinning. The chemical structure of the resulting nanofibers was characterized with FUR and H-1 NMR spectroscopy. The surface morphology and average diameter of the nanofibers were observed by SEM. Cellular response of the nanofibers was studied with mice L929 fibroblasts. Cell viability was checked on 7th day of cell culture by double staining the cells with calcein-AM and PI dye. PANI-CNT/PNIPAm-co-MAA composite nanofibers were shown the highest cell growth and cell viability as compared to PNIPAm-co-MAA nanofibers. Cell viability in ...
Here, we sought to develop a cell culture surface conversion technique that would not damage living cells. An alginate thin film, formed on a glass plate by spin coating of sodium alginate solution and dipping into calcium chloride solution, was used to inhibit adhesion of cells. The film could be removed by ethylenediaminetetraacetate (EDTA) at any time during cell culture, permitting observation of cellular responses to conversion of the culture surface in real time. Additionally, we demonstrated the validity of the alginate thin film coating method and the performance of the film. The thickness of the alginate thin film was controlled by varying the rotation speed during spin coating. Moreover, the alginate thin film completely inhibited the adhesion of cultured cells to the culture surface, irrespective of the thickness of the film. When the alginate thin film was removed from the culture surface by EDTA, the cultured cells adhered to the culture surface, and their morphology changed. ...
TY - JOUR. T1 - Morin sulphates/glucuronides enhance macrophage function in microgravity culture system. AU - Hsieh, C. L.. AU - Chao, P. D L. AU - Fang, Shih Hua. PY - 2005/9. Y1 - 2005/9. N2 - Background: The immune system changes significantly in astronauts during and after space flight. Although the mechanism has not been defined, it is reasonable to begin developing effective countermeasures to the physiological consequences of spaceflight, especially immunosuppression. Many studies have been published about the effect of flavonoids on immune modulation. Thus, the aim of this study was to develop whether flavonoids could be the effective countermeasures to the immunosuppression caused by microgravity. Materials and methods: We used a rotating wall vessel 3D (three-dimensional) culture system which recreates some of the culture conditions that occur during microgravity to study the effects of microgravity on the function of macrophages and assess the modulating effects of flavonoids on ...
TY - JOUR. T1 - Epigenetic Silencing of the Key Antioxidant Enzyme Catalase in Karyotypically Abnormal Human Pluripotent Stem Cells. AU - Konki, Mikko. AU - Pasumarthy, Kalyan. AU - Malonzo, Maia. AU - Sainio, Annele. AU - Valensisi, Cristina. AU - Söderström, Mirva. AU - Emani, Maheswara Reddy. AU - Stubb, Aki. AU - Närvä, Elisa. AU - Ghimire, Bishwa. AU - Laiho, Asta. AU - Järveläinen, Hannu. AU - Lahesmaa, Riitta. AU - Lähdesmäki, Harri. AU - Hawkins, R. David. AU - Lund, Riikka J.. PY - 2016/2/25. Y1 - 2016/2/25. N2 - Epigenomic regulation is likely to be important in the maintenance of genomic integrity of human pluripotent stem cells, however, the mechanisms are unknown. We explored the epigenomes and transcriptomes of human pluripotent stem cells before and after spontaneous transformation to abnormal karyotypes and in correlation to cancer cells. Our results reveal epigenetic silencing of Catalase, a key regulator of oxidative stress and DNA damage control in abnormal cells. Our ...
SAN DIEGO, December 15, 2017 - ViaCyte, Inc., a privately-held regenerative medicine company, today announced that the California Institute for Regenerative Medicine (CIRM) approved a grant of $1.4 million to support the initial development of immune-evasive pluripotent stem cell lines. The focus of the project will be to genetically engineer the Companys CyT49 pluripotent stem cell line. ViaCytes proprietary CyT49 cell line is well characterized and has been used to manufacture cell replacement product candidates that have been reviewed and allowed for use in clinical trials by multiple regulatory authorities.. One of the main challenges in developing an off-the-shelf cell therapy is the potential for immune rejection of implanted cells. Genetic engineering of a pluripotent stem cell line may make it possible to generate cell therapies from a single source that will not be rejected by the immune system. For diabetes, pluripotent stem cells have the potential of providing an unlimited supply ...
TY - JOUR. T1 - CXCR2 and its related ligands play a novel role in supporting the pluripotency and proliferation of human pluripotent stem cells. AU - Jung, Ji Hye. AU - Lee, Seung Jin. AU - Kim, Jihea. AU - Lee, Songhee. AU - Sung, Hwa Jung. AU - An, Jungsuk. AU - Park, Yong. AU - Kim, Byung Soo. PY - 2015/4/15. Y1 - 2015/4/15. N2 - Basic fibroblast growth factor (bFGF) is a crucial factor sustaining human pluripotent stem cells (hPSCs). We designed this study to search the substitutive factors other than bFGF for the maintenance of hPSCs by using human placenta-derived conditioned medium without exogenous bFGF (hPCCM-), containing chemokine (C-X-C motif) receptor 2 (CXCR2) ligands, including interleukin (IL)-8 and growth-related oncogene α (GROα), which were developed on the basis of our previous studies. First, we confirmed that IL-8 and/or GROα play independent roles to preserve the phenotype of hPSCs. Then, we tried CXCR2 blockage of hPSCs in hPCCM- and verified the significant decrease ...
This protocol generates oligodendrocyte progenitor cells from human pluripotent stem cells. The cells can subsequently be isolated by cell sorting for myelination studies, or they can be terminally differentiated to MBP+ oligodendrocytes. In the CNS, oligodendrocytes act as the myelinating cells. Oligodendrocytes have been identified to be key players in several neurodegenerative disorders. This protocol describes a robust, fast and reproducible differentiation protocol to generate human oligodendrocytes from pluripotent stem cells (PSCs) using a chemically defined, growth factor-rich medium. Within 8 d, PSCs differentiate into paired box 6-positive (PAX6+) neural stem cells, which give rise to OLIG2+ progenitors by day 12. Oligodendrocyte lineage transcription factor 2-positive (OLIG2+) cells begin to express the transcription factor NKX2.2 around day 18, followed by SRY-box 10 (SOX10) around day 40. Oligodendrocyte progenitor cells (OPCs) that are positive for the cell surface antigen recognized by
Cell culture[edit]. Another technique is use of cell cultures to grow vaccine strains; such as genetically engineering ... Cell culture (cell-based) manufacturing technology can be applied to influenza vaccines as they are with most viral vaccines ... In contrast, cell culture manufacturing technology can be applied to influenza vaccines as they are with most viral vaccines (e ... AVI Bio Pharma Inc. has evidence of inhibition of multiple subtypes of influenza A virus in cell culture with Morpholino ...
Introduction to Cell and Tissue Culture: Theory and Technique. Plenum Press. New York City and London.. ... The decrease in viable cells after plating is due to "anchorage-dependence"--cells must attach to the bottom of the culture ... cells inoculated}}}}\times 100} The first method is more accurate. Cell growth in culture generally undergoes a decline after ... cells plated on day 0 × 100 {\displaystyle \mathrm {PE} ={\frac {\#\,{\text{cells on day 1}}}{\#\,{\text{cells plated on day 0 ...
They were the first calves to be produced using standard cell-culturing techniques. In 2001, Brazil cloned their first heifer, ... 24 January 2018). "Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer". Cell (journal). doi:10.1016/j.cell.2018.01.020 ... However, the cloning was done from an embryo cell, while the sheep Dolly in 1996 was cloned from an adult cell. The first mouse ... "Electrostimulated cell fusion in cell engineering". Biofizika. 32 (5): viii-xi. Nowak, Rachel (3 November 2008) Cloning ' ...
For this technique, suspension cultured cells of tobacco (e.g.: NT1 or BY2 cell lines of Nicotiana tabacum) are immobilised by ... Transient expression in cultured plant cell packs is a new procedure, recently patented by the Fraunhofer Institute in Aachen, ... or even in cultures of plant cells, in order to produce a desired protein. In the method a suspension of Agrobacterium ... plant cell packs), whereafter the bacteria transfer the desired gene into the plant cells via transfer of T-DNA. The main ...
Until the 2014 discovery that human norovirus could be cultured in B cells, techniques. Like all noroviruses, MNV has a linear ... "Enteric bacteria promote human and mouse norovirus infection of B cells". Science. 346 (6210): 755-759. doi:10.1126/science. ...
A study of biopsy techniques and cell culturing techniques from extraembryonic membrane. Clin.Genet., 6, 294-306 Meena ... Development of techniques for early sampling of foetal cells. Acta Pathologica Microbiologic. Scandinavia 73: 7377 Hahnemann, N ...
Freshney, Ian R. Culture of Animal Cells: A manual of basic technique. John Wiley & Sons, Inc., Hoboken, New Jersey. ISBN 978-0 ... Cells containing vector with an insert may be identified using blue/white selection by growing cells in media containing an ... Insertion of a vector into the target cell is usually called transformation for bacterial cells, transfection for eukaryotic ... The purpose of a vector which transfers genetic information to another cell is typically to isolate, multiply, or express the ...
Sterile pyuria, is urine which contains white blood cells while appearing sterile by standard culturing techniques. It is often ... Pyuria is the condition of urine containing white blood cells or pus. Defined as the presence of 6-10 or more neutrophils per ... caused by sexually transmitted infections, such as gonorrhea, or viruses which will not grow in bacterial cultures. Sterile ...
"Biocompatibility of microplates for culturing epithelial renal cells evaluated by a microcalorimetric technique". J. Materials ... the size of the donor animal has no effect on the metabolic rate of the cell when cultured in vitro. Mammalian cells in culture ... Degradation of a culture medium in which metabolism and growth of living cells is being studied. Thus great care must be taken ... 33,000 cells is detectable. Based on this sensitivity, IMC was used to perform a large number of pioneering studies of cultured ...
... reporting on the re-implantation of culture-expanded mesenchymal stem cells using autologous platelet lysate technique". ... A second generation technique, called Carticel II uses a "fleece matrix" implanted with chondrocyte cells that is ... Then these cells are injected into the patient. These cells are held in place by a small piece of soft tissue from the tibia, ... In addition, a safety study showed safety better than surgical alternatives for this cultured cell injection procedure at a 3- ...
Cell culture techniques make it possible to produce epithelial sheets for the replacement of damaged oral mucosa. Partial- ... This early use of electrospun lattices for cell culture and tissue engineering showed that various cell types would adhere to ... It was noted that as opposed to the flattened morphology typically seen in 2D culture, cells grown on the electrospun fibers ... The cells terminally differentiate as they migrate to the surface (from the basal layer where the progenitor cells are located ...
Freshney, R. Ian (2010). Culture of animal cells : a manual of basic technique and specialized applications (6th ed.). Hoboken ... An amniocyte (literally "lamb cell") is a cell of a fetus which is suspended in the amniotic fluid. ...
Different techniques are used to automate patch clamp recordings from cells in cell culture and in vivo. This work has been ... Another technique automates the positioning of patch clamping cells in cultures. It uses a nanopipette on a precise, piezo- ... Because the cells are dissociated from one another in suspension cultures, the ionic currents in a single cell can be measured ... Because handling cells in suspension is much easier than handling cells in culture or in vivo, patch clamp recordings can be ...
... and Insect Cell Culture Techniques" (PDF). Invitrogen. Kost, T; Condreay, JP (2002). "Recombinant baculoviruses as mammalian ... Cultured mammalian cell lines such as the Chinese hamster ovary (CHO), COS, including human cell lines such as HEK and HeLa may ... Eukaryotic cell extracts may also be used in other cell-free systems, for example, the wheat germ cell-free expression systems ... Brödel AK1, Wüstenhagen DA, Kubick S (2015). "Cell-free protein synthesis systems derived from cultured mammalian cells". ...
This technique has helped improve the study of cell patterning that was not possible with traditional cell culture techniques. ... of performance A cheaper technique for fabrication that uses less energy than conventional techniques After this technique ... Once the ink has been applied to the substrate the SAM layer acts as a resist to common wet etching techniques allowing for the ... The reduction in time and DNA material are the critical advantages for using this technique. The stamps were able to be used ...
... fluorescent antibody and cell culture isolation techniques for detection of antigen". Journal of Fish Diseases. 7: 57-64. doi: ...
This procedure can be used in all cell culture laboratories and is a routinely used labeling technique. This metabolic labeling ... Stable isotope labeling with amino acids in cell culture (SILAC) is a procedure that can be done in vivo. ... The TAILS method is designed for comparison of multiple protease treated cells and control proteome cells. Samples can be ... Cell. 134 (5): 866-76. doi:10.1016/j.cell.2008.08.012. PMC 2566540 . PMID 18722006. Enoksson, Mari; Li, Jingwei; Ivancic, ...
Through tissue culture techniques a single tobacco cell was selected that contained the gene and a new plant grown from it. The ... To do this the cells undergoes a process called resolution, where during bacterial cell division one new cell receives the ... In the 1980s techniques were developed to introduce isolated chloroplasts back into a plant cell that had its cell wall removed ... The range of plants that can be transformed has increased as tissue culture techniques have been developed for different ...
Using Cod-derived Cells and Novel Culture Techniques" (2012). Theses and Dissertations (Comprehensive). Paper 1127. http:// ... It forms xenoparasitic complexes of the cell-hypertrophy tumour type, and is found in the gills of the Atlantic cod Gadus ...
Culture of Animal Cells, A Manual of Basic Technique, 5th edition, Wiley-Liss, 200- 201. Mohanta T.K., Patra J.K., Rath S.K., ... Counting Actively Metabolizing Tissue Cultured Cells, Exp. Cell. Res., 1957, 13, 341-347. Masters R.W., Animal Cell Culture, ... Masters R.W., Animal Cell Culture, Cytotoxicity and Viability Assays, Third Edition, 202-203.. ... "In Vitro Cytotoxicity Activity of Semecarpus anacardium Extract Against Hep 2 Cell Line and Vero Cell Line" (PDF). ...
Reporting on the Re-implantation of Culture-Expanded Mesenchymal Stem Cells using Autologous Platelet Lysate Technique". ... PBPC's are a blood product containing mesenchymal stem cells and is obtained by mobilizing the stem cells into the peripheral ... This technique/repair requires transplant sections of bone and cartilage. First, the damaged section of bone and cartilage is ... The main reason is that it takes a long time for the cartilage cells to adapt and mature into repair tissue. Cartilage is a ...
... reporting on the re-implantation of culture-expanded mesenchymal stem cells using autologous platelet lysate technique". Curr ... "Safety of intra-articular cell-therapy with culture-expanded stem cells in humans: a systematic literature review". ... These cells are then cultured in the patient's autologous platelet lysate, allowed to proliferate, and mixed with an antibiotic ... reporting update on the re-implantation of culture-expanded mesenchymal stem cells using autologous platelet lysate technique ...
... was an American cell biologist known for his research in cell culture techniques and carcinogenesis. Born in Greenville, South ...
As an outgrowth of the latter topic, Loeb developed the cell culture technique as applied to both normal and abnormal tissues. ... he continued his work on tissue transplantation and cell culture, as well as research on endocrine disease. Loeb was known as a ... Loeb became interested in blood coagulation and the growth properties of malignant cells. ...
Culture-media conditioned by macrophages can be used. Production in cell culture is usually preferred as the ascites technique ... single B cell culture, single cell amplification from various B cell populations and single plasma cell interrogation ... Monoclonal antibodies are typically made by cell culture that involves fusing myeloma cells with mouse spleen cells immunized ... similar to a cancer cell). This mixture of cells is then diluted and clones are grown from single parent cells on microtitre ...
... neuronal cell culture techniques, and computational neurophysiology.[better source needed] She was enrolled in a doctoral ... Jenkins, Sharon Rae (2008). A Handbook of Clinical Scoring Systems for Thematic Apperceptive Techniques. New York, NY: Taylor ... however individuals with careers oriented in new media and pop culture also make appearances. Additionally, atheism and ...
If the stem cells and sebaceous gland are destroyed, there is then no possibility for regeneration of the hair follicle, and ... New diagnostic techniques, such as trichoscopy may be used for non-invasive differential diagnosis of cicatricial alopecia. ... In addition, if pustules are present, cultures are taken to identify which microbes, if any, may be contributing to the ... The goal of treatment is to decrease or eliminate the lymphocytic inflammatory cells that are attacking and destroying the hair ...
... but this disadvantage may be overcome as new techniques for cell-based rather than egg-based culture become available.[64] Cell ... Bacteria can be grown in the laboratory on nutrient culture media, but viruses need living cells in which to replicate. Many ... Hosking, Richard (1996). 日本料理用語辞典 (英文): Ingredients & Culture. Tuttle Publishing. p. 156. ISBN 978-0-8048-2042-4.. ... The sport formed part of the culture of the ancient Indians, Chinese, Greeks, and Romans, and large sums were won or lost ...
Wholesale Mataas Na Density Cell Culture Technique from China, Need to find cheap Mataas Na Density Cell Culture Technique as ... Mataas Na Density Cell Culture Technique(Kabuuang 24 Mga Produkto para sa Mataas Na Density Cell Culture Technique) ... Pakyawan Mataas Na Density Cell Culture Technique na may mataas na kalidad bilang mababang presyo / murang, isa sa Mataas Na ... Just find high-quality brands on Mataas Na Density Cell Culture Technique produce factory, You can also feedback about what you ...
The cardiovascular tissue engineering laboratory aims to develop tissue engineering and cell-based therapeutic approaches for ... New culture techniques are being used to grow and expand the number of these cells and ways of re-delivering them into the ... Cell Stem Cell 2014;14:644-57.. *A. Ahmadi, B. McNeill, B. Vulesevic, M. Kordos, L. Mesana, S. Thorn, J. M. Renaud, E. Manthorp ... Clusters of CD34+ cells expressing integrin α5 (green) after culture on a collagen matrix ...
Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological ... Colon Monoclonal Antibodies cell cell culture cell cycle cell differentiation cells cytokine gene expression gene therapy ... Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological ... The Human Leukemia Cell Line HL-60 as a Cell Culture Model To Study Neutrophil Functions and Inflammatory Cell Responses ...
... expand and cryopreserve mammalian cell lines for in vitro research experiments. Free download. ... ECACC cell culture handbook provides fundamental cell culture techniques and protocols used to successfully thaw, ... we have assembled this updated laboratory handbook of cell culture techniques. For the researcher new to cell culture, this ... Protocols for the use of induced pluripotent stem cells and for growing cells in 3D cell culture, two areas of growing ...
... a range of molecular mechanisms deconstructed by studying cancer cells in 3D models suggest that tumor cells cultured in two- ... These 3D cell culture models are particularly beneficial for investigating mechanistic processes and drug resistance in tumor ... Recent studies have demonstrated the potential of utilizing 3D cell culture models in drug discovery programs; however, it is ... Research into developing advanced tumor cell culture models in a three-dimensional (3D) architecture that more prescisely ...
... cell & molecular biology x disease/medicine x The Scientist. » techniques, culture, cell & molecular biology and disease/ ... Single-cell genome analyses reveal the amount of mutations a human brain cell will collect from its fetal beginnings until ... T-cell therapies are not just for cancer. Researchers are also advancing immunotherapy methods to protect bone marrow ... From single-cell analysis to whole-genome sequencing, this years best new products shine on many levels. ...
... environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they ... Most cells will survive removal from the natural mic- ... Basic Techniques for Cell Culturing Diane E. Harold, Wolfgang ... Some areas of research depend more heavily than others on cell culture techniques. Neuroscience is one of the areas that has ... the new trends make it feasible to employ cell culture techniques as only one of the many methods available in a small corner ...
Cell Adhesion · Cell Culture Techniques · Cell Movement · Cell Proliferation · Cells, Cultured · Dose-Response Relationship, ... stem cell · upregulation · Adipocytes · Cell Culture Techniques · Cell Differentiation · Cells, Cultured · Coculture Techniques ... Cell Culture Techniques · Cell Proliferation · Cell Survival · Cells, Cultured · Culture Media, Conditioned · ... sandwich culture · Animals · Biological Transport, Active · Cell Culture Techniques · Cell Survival · Cells, Cultured · ...
tags: cell & molecular biology x techniques x disease/medicine x culture x ... Single-cell genome analyses reveal the amount of mutations a human brain cell will collect from its fetal beginnings until ... Video: Watch Cells Crawl To Firmer Ground. By The Scientist Staff , December 11, 2016 ... From single-cell analysis to whole-genome sequencing, this years best new products shine on many levels. ...
Alternatives to commercially available cell culture insert membranes and manufacturing techniques. Agency:. Department of ... including the ability to co-culture cells with or without cell-to-cell contact, allowing for either apical or basolateral ... The use of these more complex 3D cellular models has driven the use of more complex microtiter plates, namely cell culture ... is for the scaffold to degrade and be replaced by naturally produced ECM and promote cell to cell interaction in co-culture ...
Use of cell separation and short-term culture techniques to study erythroid cell development.. Glass J, Lavidor LM, Robinson SH ... These techniques have provided a model system for the study of erythroid cells at different stages of maturation isolated from ... Cell populations highly enriched for the different stages of erythroid cell maturation were obtained by three sequential ... and separation of the residual nucleated erythroid cells as a function of size by the velocity sedimentation technique. The ...
... labeled with a variety of fluorophores using both traditional staining methods as well as immunofluorescence techniques. ... This fluorescence image gallery explores over 30 of the most common cell lines, ... Cells in Culture. Serious attempts at the culture of whole tissues and isolated cells were first undertaken in the early 1900s ... Human Brain Glioma Cells (U-118 MG) - The U-118 MG cell line is one of several cell lines derived from malignant gliomas by J. ...
... posted in General Lab Techniques: Hi I need a protocol for cell culture staining using toluidine blue. I am using chondrocytes ... I need a protocol for cell culture staining using toluidine blue. I am using chondrocytes in 24 well culture plates ... Toluidine blue staining for cell culture. Started by abohollolo, Jul 21 2010 12:21 AM ...
... and cell culture. Prerequisite: Permission of Instructor. Students should have had college biology and chemistry (BIO 181 or ...
... but they are fragile and difficult to keep alive in cell ... Adult neural stem cells are thought of as having a lot of ... New 3D Tissue Printing Technique Being Adapted to Culture Adult Neural Stem Cells. July 24th, 2014 Editors News ... but they are fragile and difficult to keep alive in cell culture media (Petri dishes), making them a challenge to study. In ... Adult neural stem cells are thought of as having a lot of potential for therapeutic applications, ...
2014)‎. Hands-on Training Workshop on Cell Culture Techniques for the Laboratory Diagnosis of Polio/Enteroviruses and Measles/ ... Hands-on Training Workshop on Cell Culture Techniques for the Laboratory Diagnosis of Polio/Enteroviruses and Measles/Rubella ...
Avoiding Cell Culture Contamination Using the Aseptic Technique Cell cultures can provide a nutritious environment to unwanted ... Find out more in our "Avoiding Cell Culture Contamination Using the Aseptic Technique" paper from Bioquell. ... Cell cultures can provide a nutritious environment to unwanted biological microorganisms. Such contamination can have a serious ...
The new technique is more than three times as effective as previous methods. ... researchers have devised a reliable way to grow a certain type of cancer cells from patients outside the body for study. ... New cell culture technique paves the way for tailor-made cancer treatments. ... The technique may also bring doctors closer to their goal of capturing cancer cells for diagnosis with a quick, non-invasive ...
Recent innovations in human cell culture exposure and test systems has allowed the development ofin vitro assay systems that ... Innovative in vitro exposure techniques that have been developed to provide direct exposure of human lung cells to inhaled ... Advances inin vitrocell culture technology may provide some of the answers. Regulatory toxicologists and health and safety ... Bakand, S. (2016). Cell culture techniques essential for toxicity testing of inhaled materials and nanomaterials in vitro. ...
Here, we sought to develop a cell culture surface conversion technique that would not damage living cells. An alginate thin ... When the alginate thin film was removed from the culture surface by EDTA, the cultured cells adhered to the culture surface, ... Finally, we achieved effective differentiation of C2C12 myoblasts into myotube cells by cell culture on the convertible culture ... Title: Development of a cell culture surface conversion technique using alginate thin film for evaluating effect upon cellular ...
Browse Illinois Research and Scholarship (Open Community) by Subject "Cell culture techniques". ... Browse Illinois Research and Scholarship (Open Community) by Subject "Cell culture techniques". Welcome to the IDEALS ... Survival and stimulation of neurite outgrowth in a serum-free culture of spiral ganglion neurons from adult mice  ... We have developed a reliable protocol for the serum-free dissociation and culture of spiral ganglion neurons from adult mice, ...
... and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. ... primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and ... cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and ... In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the ...
... combining site-directed mutagenesis and mammalian cell culture techniques to elucidate the key residues of AQP proteins ... This project will combine site-directed mutagenesis and mammalian cell culture techniques to elucidate the key residues of AQP ... combining site-directed mutagenesis and mammalian cell culture techniques to elucidate the key residues of AQP proteins ... combining site-directed mutagenesis and mammalian cell culture techniques to elucidate the key residues of AQP proteins ...
Microbial Culture Techniques), By Application (Cell Line, Virus, & End of Production Cells Testing), By End-use, And Segment ... Mycoplasma contamination of cell culture is a growing concern for most of the research scientists for decades. Mycoplasma ... Cell line testing is anticipated to exhibit highest CAGR over the forecast period due to the increasing risk of cell line ...
Yamanaka, S. Induced pluripotent stem cells: past, present, and future. Cell stem cell. 10, (6), 678-684 (2012). ... Selective Cell Elimination from Mixed 3D Culture Using a Near Infrared Photoimmunotherapy Technique. J. Vis. Exp. (109), e53633 ... Selektiv Cell Eliminering från Mixed 3D kultur med hjälp av en Near Infrared Photoimmunotherapy Technique. Kazuhide Sato1, ... Figur 2. Mål cell eliminering i 3D cell sfäroider (blandade sfäroider av A431-luc-GFP och 3T3-RFP celler). (A) NIR-PIT (2 J / ...
  • A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. (labome.org)
  • Tissue engineering can be described as a combination of cells, engineering and materials methods for use as replacement tissues (either temporary or permanent) for damaged or diseased body parts. (ottawaheart.ca)
  • In addition to enhancing transplanted cell effects, the matrix is being evaluated for its ability to address the fact that tissues of patients requiring treatment are more aged and diseased, which negatively affects the heart's ability to respond to therapy. (ottawaheart.ca)
  • Other research being performed in the lab includes the investigation of biomaterial scaffolds and stem/progenitor cell transplantation for promoting angiogenesis in the heart in order to restore blood flow to damaged tissue and improve its function. (ottawaheart.ca)
  • The main goal of the Cardiovascular Tissue Engineering Laboratory, under the direction of Erik Suuronen, PhD, is to develop tissue engineering and cell-based therapeutic approaches for the treatment of cardiac injury and disease. (ottawaheart.ca)
  • The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (labome.org)
  • However, the science is quite complex, and translation of the research findings into the clinic is likely to require strategies to enhance the function of the therapeutic cells, and the patient's response to them. (ottawaheart.ca)
  • Strategies to improve the host stem cell response include the use of biomaterials designed to mobilize and recruit these repair cells. (ottawaheart.ca)
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