A partitioning within cells due to the selectively permeable membranes which enclose each of the separate parts, e.g., mitochondria, lysosomes, etc.
Computers in which quantities are represented by physical variables; problem parameters are translated into equivalent mechanical or electrical circuits as an analog for the physical phenomenon being investigated. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A form of creatine kinase found in the MITOCHONDRIA.
A series of oxidative reactions in the breakdown of acetyl units derived from GLUCOSE; FATTY ACIDS; or AMINO ACIDS by means of tricarboxylic acid intermediates. The end products are CARBON DIOXIDE, water, and energy in the form of phosphate bonds.
Cytosine nucleotides which contain deoxyribose as the sugar moiety.
A glycoside obtained from Digitalis purpurea; the aglycone is digitogenin which is bound to five sugars. Digitonin solubilizes lipids, especially in membranes and is used as a tool in cellular biochemistry, and reagent for precipitating cholesterol. It has no cardiac effects.
A cyclic nucleotide phosphodiesterase subfamily that is activated by the binding of CYCLIC GMP to an allosteric domain found on the enzyme. Multiple enzyme variants of this subtype can be produced due to multiple alternative mRNA splicing. The subfamily is expressed in a broad variety of tissues and may play a role in mediating cross-talk between CYCLIC GMP and CYCLIC CMP pathways. Although the type 2 enzymes are classified as 3',5'-cyclic-AMP phosphodiesterases (EC 3.1.4.17), members of this class have additional specificity for CYCLIC GMP.
Inhibitor of phosphodiesterases.
Stable carbon atoms that have the same atomic number as the element carbon, but differ in atomic weight. C-13 is a stable carbon isotope.
A metabolic process that converts GLUCOSE into two molecules of PYRUVIC ACID through a series of enzymatic reactions. Energy generated by this process is conserved in two molecules of ATP. Glycolysis is the universal catabolic pathway for glucose, free glucose, or glucose derived from complex CARBOHYDRATES, such as GLYCOGEN and STARCH.
Any spaces or cavities within a cell. They may function in digestion, storage, secretion, or excretion.
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
An ester of glucose with phosphoric acid, made in the course of glucose metabolism by mammalian and other cells. It is a normal constituent of resting muscle and probably is in constant equilibrium with fructose-6-phosphate. (Stedman, 26th ed)
An enzyme that catalyzes the formation of glycerol 3-phosphate from ATP and glycerol. Dihydroxyacetone and L-glyceraldehyde can also act as acceptors; UTP and, in the case of the yeast enzyme, ITP and GTP can act as donors. It provides a way for glycerol derived from fats or glycerides to enter the glycolytic pathway. EC 2.7.1.30.
Self-replicating cytoplasmic organelles of plant and algal cells that contain pigments and may synthesize and accumulate various substances. PLASTID GENOMES are used in phylogenetic studies.
A transferase that catalyzes formation of PHOSPHOCREATINE from ATP + CREATINE. The reaction stores ATP energy as phosphocreatine. Three cytoplasmic ISOENZYMES have been identified in human tissues: the MM type from SKELETAL MUSCLE, the MB type from myocardial tissue and the BB type from nervous tissue as well as a mitochondrial isoenzyme. Macro-creatine kinase refers to creatine kinase complexed with other serum proteins.
Basic functional unit of plants.
The rate dynamics in chemical or physical systems.
Large and highly vacuolated cells possessing many chloroplasts occuring in the interior cross-section of leaves, juxtaposed between the epidermal layers.
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
Derivatives of GLUTAMIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the 2-aminopentanedioic acid structure.
Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.
A non-essential amino acid present abundantly throughout the body and is involved in many metabolic processes. It is synthesized from GLUTAMIC ACID and AMMONIA. It is the principal carrier of NITROGEN in the body and is an important energy source for many cells.
A CALCIUM and CALMODULIN-dependent cyclic nucleotide phosphodiesterase subfamily. The three members of this family are referred to as type 1A, type 1B, and type 1C and are each product of a distinct gene. In addition, multiple enzyme variants of each subtype can be produced due to multiple alternative mRNA splicing. Although the type 1 enzymes are classified as 3',5'-cyclic-AMP phosphodiesterases (EC 3.1.4.17), some members of this class have additional specificity for CYCLIC GMP.
The two types of spaces between which water and other body fluids are distributed: extracellular and intracellular.
Derivatives of BUTYRIC ACID that contain one or more amino groups attached to the aliphatic structure. Included under this heading are a broad variety of acid forms, salts, esters, and amides that include the aminobutryrate structure.
Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.
Endocytic/exocytic CELL MEMBRANE STRUCTURES rich in glycosphingolipids, cholesterol, and lipid-anchored membrane proteins that function in ENDOCYTOSIS (potocytosis), transcytosis, and SIGNAL TRANSDUCTION. Caveolae assume various shapes from open pits to closed vesicles. Caveolar coats are composed of CAVEOLINS.
Salts and derivatives of acetoacetic acid.
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
The mitochondria of the myocardium.
Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
Method for assessing flow through a system by injection of a known quantity of radionuclide into the system and monitoring its concentration over time at a specific point in the system. (From Dorland, 28th ed)
A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.
A genus of ascomycetous fungi, family Sordariaceae, order SORDARIALES, comprising bread molds. They are capable of converting tryptophan to nicotinic acid and are used extensively in genetic and enzyme research. (Dorland, 27th ed)
Mitochondria in hepatocytes. As in all mitochondria, there are an outer membrane and an inner membrane, together creating two separate mitochondrial compartments: the internal matrix space and a much narrower intermembrane space. In the liver mitochondrion, an estimated 67% of the total mitochondrial proteins is located in the matrix. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p343-4)
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
An intermediate compound in the metabolism of carbohydrates, proteins, and fats. In thiamine deficiency, its oxidation is retarded and it accumulates in the tissues, especially in nervous structures. (From Stedman, 26th ed)
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)
Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.
ATP:pyruvate 2-O-phosphotransferase. A phosphotransferase that catalyzes reversibly the phosphorylation of pyruvate to phosphoenolpyruvate in the presence of ATP. It has four isozymes (L, R, M1, and M2). Deficiency of the enzyme results in hemolytic anemia. EC 2.7.1.40.
An enzyme that catalyzes the conversion of ATP and a D-hexose to ADP and a D-hexose 6-phosphate. D-Glucose, D-mannose, D-fructose, sorbitol, and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase. (From Enzyme Nomenclature, 1992) EC 2.7.1.1.
The area within CELLS.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Inorganic salts of phosphoric acid.
Treatment process involving the injection of fluid into an organ or tissue.
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.
A hemoflagellate subspecies of parasitic protozoa that causes nagana in domestic and game animals in Africa. It apparently does not infect humans. It is transmitted by bites of tsetse flies (Glossina).
Derivatives of formic acids. Included under this heading are a broad variety of acid forms, salts, esters, and amides that are formed with a single carbon carboxy group.
Striated muscle cells found in the heart. They are derived from cardiac myoblasts (MYOBLASTS, CARDIAC).
Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
Biosynthesis of GLUCOSE from nonhexose or non-carbohydrate precursors, such as LACTATE; PYRUVATE; ALANINE; and GLYCEROL.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
Electron transfer through the cytochrome system liberating free energy which is transformed into high-energy phosphate bonds.
The synthesis by organisms of organic chemical compounds, especially carbohydrates, from carbon dioxide using energy obtained from light rather than from the oxidation of chemical compounds. Photosynthesis comprises two separate processes: the light reactions and the dark reactions. In higher plants; GREEN ALGAE; and CYANOBACTERIA; NADPH and ATP formed by the light reactions drive the dark reactions which result in the fixation of carbon dioxide. (from Oxford Dictionary of Biochemistry and Molecular Biology, 2001)
Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals.
Plant cell inclusion bodies that contain the photosynthetic pigment CHLOROPHYLL, which is associated with the membrane of THYLAKOIDS. Chloroplasts occur in cells of leaves and young stems of plants. They are also found in some forms of PHYTOPLANKTON such as HAPTOPHYTA; DINOFLAGELLATES; DIATOMS; and CRYPTOPHYTA.
The excitable plasma membrane of a muscle cell. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Compounds which inhibit or antagonize the biosynthesis or actions of phosphodiesterases.
A nonreducing disaccharide composed of GLUCOSE and FRUCTOSE linked via their anomeric carbons. It is obtained commercially from SUGARCANE, sugar beet (BETA VULGARIS), and other plants and used extensively as a food and a sweetener.
A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.
The chemical reactions involved in the production and utilization of various forms of energy in cells.
Specific particles of membrane-bound organized living substances present in eukaryotic cells, such as the MITOCHONDRIA; the GOLGI APPARATUS; ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
Enzymes that catalyze the dehydrogenation of GLYCERALDEHYDE 3-PHOSPHATE. Several types of glyceraldehyde-3-phosphate-dehydrogenase exist including phosphorylating and non-phosphorylating varieties and ones that transfer hydrogen to NADP and ones that transfer hydrogen to NAD.
One of the non-essential amino acids commonly occurring in the L-form. It is found in animals and plants, especially in sugar cane and sugar beets. It may be a neurotransmitter.
Complex sets of enzymatic reactions connected to each other via their product and substrate metabolites.
A species of ascomycetous fungi of the family Sordariaceae, order SORDARIALES, much used in biochemical, genetic, and physiologic studies.
Detergent-insoluble CELL MEMBRANE components. They are enriched in SPHINGOLIPIDS and CHOLESTEROL and clustered with glycosyl-phosphatidylinositol (GPI)-anchored proteins.
A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.
A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, and sweetening agent.
The rate at which oxygen is used by a tissue; microliters of oxygen STPD used per milligram of tissue per hour; the rate at which oxygen enters the blood from alveolar gas, equal in the steady state to the consumption of oxygen by tissue metabolism throughout the body. (Stedman, 25th ed, p346)
Drugs that selectively bind to and activate beta-adrenergic receptors.
The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.
The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A non-essential amino acid naturally occurring in the L-form. Glutamic acid is the most common excitatory neurotransmitter in the CENTRAL NERVOUS SYSTEM.
The lower right and left chambers of the heart. The right ventricle pumps venous BLOOD into the LUNGS and the left ventricle pumps oxygenated blood into the systemic arterial circulation.
A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.
Elements of limited time intervals, contributing to particular results or situations.
A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed)
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Theoretical representations that simulate the behavior or activity of the cardiovascular system, processes, or phenomena; includes the use of mathematical equations, computers and other electronic equipment.
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.
Isopropyl analog of EPINEPHRINE; beta-sympathomimetic that acts on the heart, bronchi, skeletal muscle, alimentary tract, etc. It is used mainly as bronchodilator and heart stimulant.
A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.
An essential amino acid that is physiologically active in the L-form.
The monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose sugar, and a phosphate group. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Salts or esters of LACTIC ACID containing the general formula CH3CHOHCOOR.
The tendency of a gas or solute to pass from a point of higher pressure or concentration to a point of lower pressure or concentration and to distribute itself throughout the available space. Diffusion, especially FACILITATED DIFFUSION, is a major mechanism of BIOLOGICAL TRANSPORT.
An enzyme of the lyase class that catalyzes the formation of CYCLIC AMP and pyrophosphate from ATP. EC 4.6.1.1.
PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides.
Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
The movement of materials across cell membranes and epithelial layers against an electrochemical gradient, requiring the expenditure of metabolic energy.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

Apontic binds the translational repressor Bruno and is implicated in regulation of oskar mRNA translation. (1/4939)

The product of the oskar gene directs posterior patterning in the Drosophila oocyte, where it must be deployed specifically at the posterior pole. Proper expression relies on the coordinated localization and translational control of the oskar mRNA. Translational repression prior to localization of the transcript is mediated, in part, by the Bruno protein, which binds to discrete sites in the 3' untranslated region of the oskar mRNA. To begin to understand how Bruno acts in translational repression, we performed a yeast two-hybrid screen to identify Bruno-interacting proteins. One interactor, described here, is the product of the apontic gene. Coimmunoprecipitation experiments lend biochemical support to the idea that Bruno and Apontic proteins physically interact in Drosophila. Genetic experiments using mutants defective in apontic and bruno reveal a functional interaction between these genes. Given this interaction, Apontic is likely to act together with Bruno in translational repression of oskar mRNA. Interestingly, Apontic, like Bruno, is an RNA-binding protein and specifically binds certain regions of the oskar mRNA 3' untranslated region.  (+info)

Membrane-tethered Drosophila Armadillo cannot transduce Wingless signal on its own. (2/4939)

Drosophila Armadillo and its vertebrate homolog beta-catenin are key effectors of Wingless/Wnt signaling. In the current model, Wingless/Wnt signal stabilizes Armadillo/beta-catenin, which then accumulates in nuclei and binds TCF/LEF family proteins, forming bipartite transcription factors which activate transcription of Wingless/Wnt responsive genes. This model was recently challenged. Overexpression in Xenopus of membrane-tethered beta-catenin or its paralog plakoglobin activates Wnt signaling, suggesting that nuclear localization of Armadillo/beta-catenin is not essential for signaling. Tethered plakoglobin or beta-catenin might signal on their own or might act indirectly by elevating levels of endogenous beta-catenin. We tested these hypotheses in Drosophila by removing endogenous Armadillo. We generated a series of mutant Armadillo proteins with altered intracellular localizations, and expressed these in wild-type and armadillo mutant backgrounds. We found that membrane-tethered Armadillo cannot signal on its own; however it can function in adherens junctions. We also created mutant forms of Armadillo carrying heterologous nuclear localization or nuclear export signals. Although these signals alter the subcellular localization of Arm when overexpressed in Xenopus, in Drosophila they have little effect on localization and only subtle effects on signaling. This supports a model in which Armadillo's nuclear localization is key for signaling, but in which Armadillo intracellular localization is controlled by the availability and affinity of its binding partners.  (+info)

Polarized distribution of Bcr-Abl in migrating myeloid cells and co-localization of Bcr-Abl and its target proteins. (3/4939)

Bcr-Abl plays a critical role in the pathogenesis of Philadelphia chromosome-positive leukemia. Although a large number of substrates and interacting proteins of Bcr-Abl have been identified, it remains unclear whether Bcr-Abl assembles multi-protein complexes and if it does where these complexes are within cells. We have investigated the localization of Bcr-Abl in 32D myeloid cells attached to the extracellular matrix. We have found that Bcr-Abl displays a polarized distribution, colocalizing with a subset of filamentous actin at trailing portions of migrating 32D cells, and localizes on the cortical F-actin and on vesicle-like structures in resting 32D cells. Deletion of the actin binding domain of Bcr-Abl (Bcr-AbI-AD) dramatically enhances the localization of Bcr-Abl on the vesicle-like structures. These distinct localization patterns of Bcr-Abl and Bcr-Abl-AD enabled us to examine the localization of Bcr-Abl substrate and interacting proteins in relation to Bcr-Abl. We found that a subset of biochemically defined target proteins of Bcr-Abl redistributed and co-localized with Bcr-Abl on F-actin and on vesicle-like structures. The co-localization of signaling proteins with Bcr-Abl at its sites of localization supports the idea that Bcr-Abl forms a multi-protein signaling complex, while the polarized distribution and vesicle-like localization of Bcr-Abl may play a role in leukemogenesis.  (+info)

Plasma membrane recruitment of RalGDS is critical for Ras-dependent Ral activation. (4/4939)

In COS cells, Ral GDP dissociation stimulator (RalGDS)-induced Ral activation was stimulated by RasG12V or a Rap1/Ras chimera in which the N-terminal region of Rap1 was ligated to the C-terminal region of Ras but not by Rap1G12V or a Ras/Rap1 chimera in which the N-terminal region of Ras was ligated to the C-terminal region of Rap1, although RalGDS interacted with these small GTP-binding proteins. When RasG12V, Ral and the Rap1/Ras chimera were individually expressed in NIH3T3 cells, they localized to the plasma membrane. Rap1Q63E and the Ras/Rap1 chimera were detected in the perinuclear region. When RalGDS was expressed alone, it was abundant in the cytoplasm. When coexpressed with RasG12V or the Rap1/Ras chimera, RalGDS was detected at the plasma membrane, whereas when coexpressed with Rap1Q63E or the Ras/Rap1 chimera, RalGDS was observed in the perinuclear region. RalGDS which was targeted to the plasma membrane by the addition of Ras farnesylation site (RalGDS-CAAX) activated Ral in the absence of RasG12V. Although RalGDS did not stimulate the dissociation of GDP from Ral in the absence of the GTP-bound form of Ras in a reconstitution assay using the liposomes, RalGDS-CAAX could stimulate it without Ras. RasG12V activated Raf-1 when they were coexpressed in Sf9 cells, whereas RasG12V did not affect the RalGDS activity. These results indicate that Ras recruits RalGDS to the plasma membrane and that the translocated RalGDS induces the activation of Ral, but that Rap1 does not activate Ral due to distinct subcellular localization.  (+info)

ETO-2, a new member of the ETO-family of nuclear proteins. (5/4939)

The t(8;21) is associated with 12-15% of acute myelogenous leukemias of the M2 subtype. The translocation results in the fusion of two genes, AML1 (CBFA2) on chromosome 21 and ETO (MTG8) on chromosome 8. AML1 encodes a DNA binding factor; the ETO protein product is less well characterized, but is thought to be a transcription factor. Here we describe the isolation and characterization of ETO-2, a murine cDNA that encodes a new member of the ETO family of proteins. ETO-2 is 75% identical to murine ETO and shares very high sequence identities over four regions of the protein with ETO (domain I-III and zinc-finger). Northern analysis identifies ETO-2 transcripts in many of the murine tissues analysed and in the developing mouse embryo. ETO-2 is also expressed in myeloid and erythroid cell lines. We confirmed the nuclear localization of ETO-2 and demonstrated that domain III and the zinc-finger region are not required for nuclear localization. We further showed that a region within ETO, containing domain II, mediates dimerization among family members. This region is conserved in the oncoprotein AML-1/ETO. The recent identification of another ETO-like protein, myeloid translocation gene-related protein 1, together with the data presented here, demonstrates that at least three ETO proteins exist with the potential to form dimers in the cell nucleus.  (+info)

The amyloid precursor protein interacts with Go heterotrimeric protein within a cell compartment specialized in signal transduction. (6/4939)

The function of the beta-amyloid protein precursor (betaAPP), a transmembrane molecule involved in Alzheimer pathologies, is poorly understood. We recently reported the presence of a fraction of betaAPP in cholesterol and sphingoglycolipid-enriched microdomains (CSEM), a caveolae-like compartment specialized in signal transduction. To investigate whether betaAPP actually interferes with cell signaling, we reexamined the interaction between betaAPP and Go GTPase. In strong contrast with results obtained with reconstituted phospholipid vesicles (Okamoto et al., 1995), we find that incubating total neuronal membranes with 22C11, an antibody that recognizes an N-terminal betaAPP epitope, reduces high-affinity Go GTPase activity. This inhibition is specific of Galphao and is reproduced, in the absence of 22C11, by the addition of the betaAPP C-terminal domain but not by two distinct mutated betaAPP C-terminal domains that do not bind Galphao. This inhibition of Galphao GTPase activity by either 22C11 or wild-type betaAPP cytoplasmic domain suggests that intracellular interactions between betaAPP and Galphao could be regulated by extracellular signals. To verify whether this interaction is preserved in CSEM, we first used biochemical, immunocytochemical, and ultrastructural techniques to unambiguously confirm the colocalization of Galphao and betaAPP in CSEM. We show that inhibition of basal Galphao GTPase activity also occurs within CSEM and correlates with the coimmunoprecipitation of Galphao and betaAPP. The regulation of Galphao GTPase activity by betaAPP in a compartment specialized in signaling may have important consequences for our understanding of the physiopathological functions of betaAPP.  (+info)

NKp44, a triggering receptor involved in tumor cell lysis by activated human natural killer cells, is a novel member of the immunoglobulin superfamily. (7/4939)

Surface receptors involved in natural killer (NK) cell triggering during the process of tumor cell lysis have recently been identified. Of these receptors, NKp44 is selectively expressed by IL-2- activated NK cells and may contribute to the increased efficiency of activated NK cells to mediate tumor cell lysis. Here we describe the molecular cloning of NKp44. Analysis of the cloned cDNA indicated that NKp44 is a novel transmembrane glycoprotein belonging to the Immunoglobulin superfamily characterized by a single extracellular V-type domain. The charged amino acid lysine in the transmembrane region may be involved in the association of NKp44 with the signal transducing molecule killer activating receptor-associated polypeptide (KARAP)/DAP12. These molecules were found to be crucial for the surface expression of NKp44. In agreement with data of NKp44 surface expression, the NKp44 transcripts were strictly confined to activated NK cells and to a minor subset of TCR-gamma/delta+ T lymphocytes. Unlike genes coding for other receptors involved in NK cell triggering or inhibition, the NKp44 gene is on human chromosome 6.  (+info)

The iron transport protein NRAMP2 is an integral membrane glycoprotein that colocalizes with transferrin in recycling endosomes. (8/4939)

The natural resistance associated macrophage protein (Nramp) gene family is composed of two members in mammals, Nramp1 and Nramp2. Nramp1 is expressed primarily in macrophages and mutations at this locus cause susceptibility to infectious diseases. Nramp2 has a much broader range of tissue expression and mutations at Nramp2 result in iron deficiency, indicating a role for Nramp2 in iron metabolism. To get further insight into the function and mechanism of action of Nramp proteins, we have generated isoform specific anti-Nramp1 and anti-Nramp2 antisera. Immunoblotting experiments indicate that Nramp2 is present in a number of cell types, including hemopoietic precursors, and is coexpressed with Nramp1 in primary macrophages and macrophage cell lines. Nramp2 is expressed as a 90-100-kD integral membrane protein extensively modified by glycosylation (>40% of molecular mass). Subcellular localization studies by immunofluorescence and confocal microscopy indicate distinct and nonoverlapping localization for Nramp1 and Nramp2. Nramp1 is expressed in the lysosomal compartment, whereas Nramp2 is not detectable in the lysosomes but is expressed primarily in recycling endosomes and also, to a lower extent, at the plasma membrane, colocalizing with transferrin. These findings suggest that Nramp2 plays a key role in the metabolism of transferrin-bound iron by transporting free Fe2+ across the endosomal membrane and into the cytoplasm.  (+info)

Definition of distinct compartments in polarized Madin-Darby canine kidney (MDCK) cells for membrane-volume sorting, polarized sorting and apical recycling.
The present study suggests that the NH2-terminal amino acids of the galectin-3 leader sequence regulate cellular translocation of galectin-3, but this activity does not depend on the phosphorylation of Ser6 by casein kinase I. The function of the unusual NH2-terminal leader domain of galectin-3 was studied using site-directed mutagenesis to produce a cDNA lacking the 11 NH2-terminal amino acids (residues 2-12), which was then expressed in the galectin-3-null BT-549 human breast carcinoma cell line. Galectin-3 contains no typical signal peptide sequence and presumably is secreted by a mechanism independent of the classical secretary pathway in a manner similar to β-fibroblast growth factor and interleukin 1 (10) . It is believed that galectin-3 undergoes a post-translational modification after being synthesized on free cytoplasmic ribosomes and that the NH2-terminal Met and Ala could be acylated, thus blocking secretion and translocation of galectin-3 to the cell surface (25) . Because wild-type ...
In this study, we generated a mutant of SpCdc25 that is severely impaired in its ability to bind to the fission yeast 14-3-3 proteins (Rad 24 and Rad 25). When expressed in fission yeast, this mutant Cdc25 protein localized almost exclusively to the nucleus, in contrast to wild-type Cdc25, which localized to both the cytoplasm and the nucleus. Inhibition of Crm1-mediated nuclear export resulted in the nuclear accumulation of wild-type Cdc25, indicating that wild-type Cdc25 normally shuttles between the nucleus and the cytoplasm. Overproduction of Rad 24 caused wild-type Cdc25 to localize exclusively to the cytoplasm, whereas nuclear localization of the 14-3-3 binding mutant was not altered upon Rad 24 overproduction. Finally, cells expressing the 14-3-3 binding mutant exhibited defective G2/M checkpoint responses. Taken together, these results suggest that 14-3-3 binding regulates the intracellular compartmentalization of Cdc25 and establish that 14-3-3 binding to Cdc25 is required for fission ...
Intracellular compartmentalization through interactions among MAPKs and scaffold proteins plays an important role in the regulation of signal transduction pathways (Morrison and Davis, 2003; Kolch, 2005). For example, active MEK-ERK complexes are retained in the cytoplasm and in sites of focal adhesion through interaction with the transmembrane protein Sef (Torii et al., 2004) and paxilin (Ishibe et al., 2004), respectively. Moreover, kinase suppressor of Ras 1 (Muller et al., 2001) and the complex p14-MEK-partner 1 (Teis et al., 2002; Pullikuth et al., 2005) are recruited to the plasma membrane and the endosome, respectively, where they enhance MEK and ERK activity. On the other hand, the association between ERKs and structural nuclear proteins, such as kinetochores, may serve anchoring purposes (Shapiro et al., 1998). To the best of our knowledge, we show here for the first time that A-type lamins function as a nuclear docking platform for EKR1/2, and that NE-bound ERK1/2 contributes to the ...
Ever since the beginning of biochemical analysis, yeast has been a pioneering model for studying the regulation of eukaryotic metabolism. During the last three decades, the combination of powerful yeast genetics and genome-wide approaches has led to a more integrated view of metabolic regulation. Multiple layers of regulation, from suprapathway control to individual gene responses, have been discovered. Constitutive and dedicated systems that are critical in sensing of the intra-and extracellular environment have been identified, and there is a growing awareness of their involvement in the highly regulated intracellular compartmentalization of proteins and metabolites. This review focuses on recent developments in the field of amino acid, nucleotide, and phosphate metabolism and provides illustrative examples of how yeast cells combine a variety of mechanisms to achieve coordinated regulation of multiple metabolic pathways. Importantly, common schemes have emerged, which reveal mechanisms ...
Publications related to biomolecular condensates, phase separation, llps and more. Intracellular compartmentalization through liquid-liquid phase separation is an emerging organizing principle of cell biology. These compartments, such as the nucleolus and stress granules, are collectively known as membraneless organelles or biomolecular condensates. In vitro studies of many protein components of biomolecular condensates, such as the intrinsically disordered regions of Ddx4, FUS, and Laf-1 proteins, have revealed much about the driving forces of the phase separation process. A common approach is to investigate how the temperature at which a protein solution forms condensates-the transition temperature-responds to changes in the solution composition. We describe a method to measure the in vitro transition temperature of a sub-10 μL sample of a phase-separating solution using transmitted light microscopy. Through monitoring changes in transition temperature with solution conditions, this approach allows
By using signaling pathway- and cell type-specific responses in a combinatorial manner, the immune system signaling network is able to generate a highly diverse set of functional responses despite a limited number of cytokines, cell types, and signaling proteins. In addition to receptors that are coupled to intricate intracellular pathways, specialized cell types express their own set of receptors, and the activation of the same receptor on two different cell types can result in disparate functional responses. This allows sophisticated network behavior despite a limited repertoire of conserved signaling proteins (primarily of the Jak-Stat pathway). Modulating both these levels of the network is the organization of the immune system into distinct compartments, further refining the immune response and ensuring that it is appropriate for the tissue in which the cells resides.. The analysis of immune cell signaling at the network level can be enhanced by approaches that allow cell activities to be ...
The skin represents the outermost defense against microbial and environmental insults. The importance of cutaneous integrity is emphasized by the multitude of strategies that counteract pathogen invasion, including the physical barrier provided by keratinocytes, the production of antimicrobial mediators by skin-resident cells, and the localization of immune cells in the different layers of the skin. It has become increasingly clear that epidermis and dermis do not merely represent anatomically distinct compartments, but rather directly and specifically shape the immune milieu to support the activities of resident leukocytes. In this study, we have identified an abundant population of resident dermal γδ T cells that displays a unique phenotypic profile, survival requirements, and migratory behavior as compared with its epidermal and systemic counterparts. Our results thus expand on the concept of microcompartmental specialization of distinct immune cell subsets within the skin. They further ...
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Compartmentation is a recognised strategy for preventing fire spread and aiding swift evacuation, but little is understood about its true effectiveness. Here, Simon Ellison of fire glass specialist, Pyroguard, explains how fire spreads and why com...
Purinergic signaling has been established as an important feature of inflammation and homeostasis. The expression of a number of P2 receptor subtypes in the gut has been reported. In this study, using
Mithril is right. Think of it in terms of the factory. If you have one worker that does everything or 1000 workers that are each specialised for doing one specific thing? In which case do you have a better product. Or here is another example: A hospital full of specialist or a hospital full of GPs. Which will treat more patients ...
TY - JOUR. T1 - The subcellular compartmentalization of arginine metabolizing enzymes and their role in endothelial dysfunction. AU - Chen, Feng. AU - Lucas, Rudolf. AU - Fulton, David. N1 - Copyright: Copyright 2014 Elsevier B.V., All rights reserved.. PY - 2013. Y1 - 2013. N2 - The endothelial production of nitric oxide (NO) mediates endothelium-dependent vasorelaxation and restrains vascular inflammation, smooth muscle cell proliferation, and platelet aggregation. Impaired production of NO is a hallmark of endothelial dysfunction and promotes the development of cardiovascular disease. In endothelial cells, NO is generated by endothelial nitric oxide synthase (eNOS) through the conversion of its substrate, L-arginine to L-citrulline. Reduced access to L-arginine has been proposed as a major mechanism underlying reduced eNOS activity and NO production in cardiovascular disease. The arginases (Arg1 and Arg2) metabolize L-arginine to generate L-ornithine and urea and increased expression of ...
Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly regulated by its post-translational modifications. AnxA2 and its Tyr23-phosphorylated form (pTyr23AnxA2) are involved in malignant cell transformation, metastasis and angiogenesis. Here, we show that H2O2 exerts rapid, simultaneous and opposite effects on the Tyr23 phosphorylation status of AnxA2 in two distinct compartments of rat pheochromocytoma (PC12) cells. Reactive oxygen species induce dephosphorylation of pTyr23AnxA2 located in the PML bodies of the nucleus, whereas AnxA2 associated with F-actin at the cell cortex is Tyr23 phosphorylated. The H2O2-induced responses in both compartments are transient and the pTyr23AnxA2 accumulating at the cell cortex is subsequently incorporated into vesicles and then released to the extracellular space. Blocking nuclear export by leptomycin B does not affect the nuclear pool of pTyr23AnxA2, but increases the amount of total AnxA2 ...
The biogenesis maintenance and function of primary cilia are controlled through intraflagellar transport (IFT) driven by two kinesin-2 family members the heterotrimeric KIF3A/KIF3B/KAP complex and the homodimeric KIF17 engine1 2 How these motors and their cargoes gain access to the ciliary compartment is poorly understood. Consistent with this cytoplasmic manifestation of GTP-locked Ran(G19V) disrupts the gradient and abolishes ciliary access of KIF17. Furthermore KIF17 interacts with importin-β2 in a manner dependent on the CLS and inhibited by Ran-GTP. We propose that Ran plays a global part in regulating cellular compartmentalization by controlling the shuttling of cytoplasmic proteins into nuclear and ciliary compartments. The development of the primary cilium a Fructose microtubule-based organelle projecting from the surface of nearly all cells has been proposed to be a result of evolved engine protein-based trafficking unique to eukaryotic cells3. Main cilia play important tasks in ...
A central issue in cell biology is the physico-chemical basis of organelle biogenesis in intracellular trafficking pathways, its most impressive manifestation being the biogenesis of Golgi cisternae. At a basic level, such morphologically and chemically distinct compartments should arise from an interplay between the molecular transport and chemical maturation. Here, we formulate analytically tractable, minimalist models, that incorporate this interplay between transport and chemical progression in physical space, and explore the conditions for de novo biogenesis of distinct cisternae. We propose new quantitative measures that can discriminate between the various models of transport in a qualitative manner-this includes measures of the dynamics in steady state and the dynamical response to perturbations of the kind amenable to live-cell imaging.. ...
Contains nuclear envelope, nucleoli, chromatin, and distinct compartments rich in specific protein sets. Gene-containing control center of the cell. COntains the genetic library with blueprints for nearly all cellular proteins. Dictates the kinds and amounts of proteins to be synthesized. ...
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state ...
海词词典,最权威的学习词典,专业出版pre-lysosome compartment是什么意思,pre-lysosome compartment的用法,pre-lysosome compartment翻译和读音等详细讲解。海词词典:学习变容易,记忆很深刻。
Intracellular fluid is the fluid that exists within the cells of multi-celled organisms. The intracellular fluid is therefore stored within the intracellular compartments of the body
Loss of intracellular compartmentalization of potassium is a biochemical feature of Alzheimers disease indicating a loss of membrane integrity and mitochondrial dysfunction. We examined potassium and rubidium (a biological proxy for potassium) in brain tissue, blood fractions and cerebrospinal fluid from Alzheimers disease and healthy control subjects to investigate the diagnostic potential of these two metal ions. We found that both potassium and rubidium levels were significantly decreased across all intracellular compartments in the Alzheimers disease brain. Serum from over 1000 participants in the Australian Imaging, Biomarkers and Lifestyle Flagship Study of Ageing (AIBL), showed minor changes according to disease state. Potassium and rubidium levels in erythrocytes and cerebrospinal fluid were not significantly different according to disease state, and rubidium was slightly decreased in Alzheimers disease patients compared to healthy controls. Our data provides evidence that contrasts the
Rabbit Polyclonal to HDAC5 (phospho-Ser259) however, not postabsorptively (i.e., higher blood sugar availability) (16). Also, muscle tissue acetylcarnitine correlated adversely with FAO in the postabsorptive state (71), whereas plasma acetylcarnitine correlated with plasma glucose levels in the postprandial state (72). In light of these data, the question is usually interesting if CrAT actually mementos FA-derived acetyl-CoA over glucose-derived acetyl-CoA because this may imply intracellular compartmentalization of acetyl-CoA (68). Furthermore, glucose-derived acetyl-CoA could be carboxylated by ACC, creating the CPT1 inhibitor malonyl-CoA. Direct ramifications of FAO-derived acetyl-CoA on insulin action are unknown. C4-OH-carnitine (i.e., the carnitine ester of 3-hydroxybutyrate) has been proposed to cause insulin resistance: hepatic overexpression of malonyl-CoA decarboxylase in rats on an HFD reversed whole-body, liver, and muscles insulin level of resistance while just decreasing ...
Authors: van Kessel KP, Bestebroer J, van Strijp JA. Initial elimination of invading Staphylococcus aureus from the body is mediated by professional phagocytes. The neutrophil is the major phagocyte of the innate immunity and plays a key role in the host defense against staphylococcal infections. Opsonization of the bacteria with immunoglobulins and complement factors enables efficient recognition by the neutrophil that subsequently leads to intracellular compartmentalization and killing. Here, we provide a review ofthe key processes evolved in neutrophil-mediated phagocytosis of S. aureus and briefly describe killing. As S. aureus is not helpless against the professional phagocytes, we will also highlight its immune evasion arsenal related to phagocytosis.. ...
TY - JOUR. T1 - Lack of association between autism and four heavy metal regulatory genes. AU - Owens, Sarah E.. AU - Summar, Marshall L.. AU - Ryckman, Kelli K.. AU - Haines, Jonathan L.. AU - Reiss, Sara. AU - Summar, Samantha R.. AU - Aschner, Michael. PY - 2011/12/1. Y1 - 2011/12/1. N2 - Autism is a common neurodevelopmental disorder with genetic and environmental components. Though unproven, genetic susceptibility to high mercury (Hg) body burden has been suggested as an autism risk factor in a subset of children. We hypothesized that exposure to safe Hg levels could be implicated in the etiology of autism if genetic susceptibility altered Hgs metabolism or intracellular compartmentalization. Genetic sequences of four genes implicated in the transport and response to Hg were screened for variation and association with autism. LAT1 and DMT1 function in Hg transport, and Hg exposure induces MTF1 and MT1a. We identified and characterized 74 variants in MT1a, DMT1, LAT1 and MTF1. ...
The extracellular sunscreen scytonemin is the most common and widespread indole-alkaloid among cyanobacteria. Previous research using the cyanobacterium Nostoc punctiforme ATCC 29133 revealed a unique 18-gene cluster (NpR1276 to NpR1259 in the N. punctiforme genome) involved in the biosynthesis of scytonemin. We provide further genomic characterization of these genes in N. punctiforme and extend it to homologous regions in other cyanobacteria. Six putative genes in the scytonemin gene cluster (NpR1276 to NpR1271 in the N. punctiforme genome), with no previously known protein function and annotated in this study as scyA to scyF, are likely involved in the assembly of scytonemin from central metabolites, based on genetic, biochemical, and sequence similarity evidence. Also in this cluster are redundant copies of genes encoding for aromatic amino acid biosynthetic enzymes. These can theoretically lead to tryptophan and the tyrosine precursor, p-hydroxyphenylpyruvate, (expected biosynthetic precursors of
Our genomic DNA is packaged into distinct compartments that support essential, highly conserved cellular processes. Paradoxically, the chromatin proteins that establish and maintain these compartments are strikingly unconserved. Sequence divergence and whole-gene turnover is common even between closely related species. Although chromatin dysfunction is a hallmark of cancer, these plastic but essential components of chromatin biology have received minimal attention. I combine comparative genomics, evolutionary genetics, and cell biology to gain insight into the causes and functional consequences of this evolution. I first set out to increase our compendium of these plastic genes. I computationally searched 12 sequenced fruit fly (Drosophila) genomes spanning 40 million years of evolution for new members of the Heterochromatin Protein 1 (HP1) gene family. I discovered 22 additional HP1 genes that encode unprecedented structural diversity, species-specificity, and sex-biased expression. I ...
The tried-and-true Campus 33L gets a passing grade as the ultimate back-to-school backpack. This roomy 33-litre backpack features four distinct compartments including a large top compartment with a padded laptop sleeve and room for books and binders, a top fleece lined sunglass pocket, a slim organizer pocket, and a handy insulated cooler pocket for lunch and snacks.. ...
According to the study by, Columbia University College of Physicians & Surgeons, repeated administration of this methamphetamine (5 mg/kg administered three times at 2-h intervals) leads to a transition from horizontal hyperlocomotion to excessive orofacial stereotypy (taffy pulling) only in wild type and D3 mutants. In both genotypes, this transition is accompanied by a change in the relative ratios of striatal neuronal activation in two neurochemically distinct compartments, with striosomal neuronal activation exceeding that of the striatal matrix during stereotypy. Both the stereotypic response to METH and the associated predominant activation of neurons located in striosomes require D2-receptor expression. These studies indicate a differential requirement for D1- and D2-like receptor activation in mediating the effects of METH on cognitive and motor function(7). Other indicated that the D3 Dopamine Receptor Hypothesis suggests D3 antagonists could prevent sensitization, and may interrupt ...
ePIC (electronic Publication Information Center) is the official repository for publications and presentations of Alfred Wegener Institute for Polar and Marine Research (AWI)
Compartmentation of glucose 6-phosphate in hepatocytes.: Rat hepatocytes were incubated with 14C-labelled hexoses, and the specific radioactivities of glucose 6
A polarized arrangement of neuronal microtubule arrays is the foundation of membrane trafficking and subcellular compartmentalization. Conserved among both inve
Liquid to be treated flows into a combined sedimentation and thickening tank. The upper part of this tank is a sedimentation zone in which the liquid passes through parallel flow paths between downwardly inclined separator plates below which the solids fall to the bottom of the tank into the thickening zone whereat the solids are stirred and removed by a suitable stirring and scraping device. The liquids flowing with the solids through the parallel paths are removed at the end of said paths and delivered directly to the top of the sedimentation zone thus leaving the thickening zone essentially flow-free. Flocculants may be added to the water to be treated in a separate flocculation compartment arranged upstream from the sedimentation compartment. A separate destabilization compartment may be provided upstream from the flocculation compartment. A square sedimentation zone may merge smoothly into a circular thickening zone. The thickening zone may extend beneath the flocculation compartment for
View Notes - BICD 110 � Lec 6 from BICD BICD 110 at UCSD. BICD 110 Lec 6 (4/15/10) Intracellular compartments and protein sorting I. Cell is highly compartmentalized a. Isolate reactions by
half-life (T1/2) the time taken a drug to clear from the highest concentration to half this level. Drugs have different half-lives in different compartments (ie half-life in blood can be different from the half-life inside a cell). It take 5 …. ...
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Somites are transient, segmentally organized structures. In the vertebrate embryo, the somites contribute to multiple tissues, including the axial skeleton, skeletal and smooth muscles, dorsal dermis, tendons, ligaments, cartilage and adipose tissue. The somites also determine the migration paths of trunk neural crest cells and spinal nerve axons.. As the primitive streak regresses and the neural folds begin to gather at the center of the embryo, the paraxial mesoderm separates into blocks of cells called somites. These structures are formed by budding off as epithelial spheres from the cranial end of the unsegmented paraxial mesoderm that lies on either side of the neural tube.. The total number of somites formed is species-specific (38-39 in humans, 50 in chickens, 65 in mice) and is used as an indicator of embryonic developmental stages. Once formed, the epithelial somite is patterned rapidly into distinct compartments that subsequently give rise to distinct cell lineages. In response to ...
Accumulating evidence indicates that intracellular trafficking and dynamic changes in the subcellular distribution and compartmentalization of type II nuclear hormone receptors play an important role in their biological functions (Baumann et al., 1999; Baumann et al., 2001b; Hager et al., 2002). In contrast to previous assumptions that most type II nuclear hormone receptors are constitutively present in the nucleus, studies using live cell imaging have shown that these receptors are mobile and can shuttle between the cytoplasm and the nucleus (Barsony and Prufer, 2002; Baumann et al., 2001a; Baumann et al., 2001b). Moreover, receptors may continuously exchange between subnuclear macromolecular complexes. The mechanisms that mediate these processes and their biological significance are largely unknown. In this report, we have identified CAP350 as a centrosome-associated protein and a previously undescribed interacting partner of PPARα and have shown that CAP350 recruits PPARα to discrete ...
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other ...
How is it our brains-tangible entities-are able to store memories of intangible sights, sounds, emotions and ideas? What draws you toward one person, but repels you from another? My lab tries to answer these questions. Specifically, we explore how cyclic nucleotide signaling controls the formation of social memories and social interactions, and how alterations in this cascade might contribute to social deficits that are seen in patients with neurodevelopmental, psychiatric, and age-related disease. To do this, we identify molecular changes in tissue from patients and then mimic those changes in rodents in order to determine their behavioral, biochemical, and molecular consequences. We also use in vitro approaches to determine how mutations may change enzymatic activity or the subcellular compartmentalization of enzymes. Studies are conducted during development, early adulthood, and late adulthood in order to understand factors that modulate resiliency of the brain as well as those that trigger ...
Both Mg2+ and Ca2+ regularly stabilize membranes by the cross-linking of carboxylated and phosphorylated head groups of lipids. This allows for cohesive architecture of cells but also allows for optimal cellular communication. When Mg levels are low it can cause bad signaling between cells and allow the cellular terroir to be more prone to cancer. After all, cancer is a disease of genetic chaos and bad signaling. To make this clear…..Mg is the major co factor in most key critical enzymatic steps activating proteins in our cells. The transport of Mg2+ between intracellular compartments may be a major part of regulating enzyme activity. The interaction of Mg2+ with proteins must also be considered for the transport of the ion across biological membranes as well. So if magnesium concentration or transport are altered you can bet your ass bad things are going to follow.. Speaking of bad things lets talk about the disease where intracellular magnesium is totally disordered. Lets talk diabetes. ...
All implants have a life expectancy that depends on several factors including the patients weight, activity level, quality of bone stock and compliance with their physicians orders. Proper implant alignment and precise positioning during surgery are also very important factors that can improve the life expectancy of an implant. Through the use of RIO, implants can be optimally aligned and positioned to ensure the longest benefit. RESTORIS MCK implants enable the treatment of one or two compartments with OA disease. With single compartment disease, a second compartment may be treated in the future if OA spreads. In addition, because very little bone is actually removed during a MAKOplasty® procedure, the implants can be replaced with another procedure such as a total knee replacement, if necessary.. ...
Endocrine and immune systems communicate through numerous anatomical and hormonal exchanges. Interactions between these systems are believed to be critical for the preservation of a homeostatic equilibrium. A disproportion or alterations in these systems, in response to illness, stress, damage and/or metabolic alterations, can lead to substantial changes in immune responsiveness and susceptibility to infections and autoimmune disease states. A greater understanding of the interplay between these systems may afford valuable insights into how interference inside these compartments may influence the hosts ability to regulate inflammatory response, disease progress or reparative response. Furthermore, a more exhaustive understanding of these pathways may certainly yield novel therapeutics and interventional approaches to control immune and systemic responses to disease, injury and stress. The interplay between the neuroendocrine and immune systems is nowadays well established. Actually, these systems use a
Buy your Wiggle Nutrition Shaker Bottle with Storage Compartment at wiggle.com.au. Our price AU$4.38. FREE delivery and in country returns available.
Proteins are co-translationally transferred into the endoplasmic reticulum (ER) and then either retained or transported to different intracellular compartments or to the extracellular space. Various molecular signals necessary for retention in the ER or targeting to different compartments have been …
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This how the bag looks when it is opened up and you can clearly see the different compartments to the bag and how spacious it is. At the top there is a little zip compartment which is where I actually put my bits of jewellery which I want to wear whilst at home. The bag then has 2 large compartments which are clear which is great so you can see exactly what you have packed and what you have missed without having to root through. It looks a little messy as everything has fallen to the bottom of the bag as it is hung up. The hook is really useful as you can just hang it on your door like I have, and pack whilst it is hanging there ...
[randpic]Continuous Ball Mill, Manufacturer, IndiaMay 15, 2021 Continuous Ball Mill. It is a cylindrical grinder having three different compartments and screen. Replaceable liners or balls are fixed inside entire mill. Balls are made of stainless s
CarDekho.com - answers for does this sedan have some hidden compartments for storage as well?. post your answer for does this sedan have some hidden compartments for storage as well?.
Creative Biostructure has developed a comprehensive protein enginereeing platform to provide custom selection services via in vitro compartments method.
Probably a very easy question, but before I take it off to see and attempt to clean it, Id like to know. Im tying to track down the 35 MPG cause. We...
Liu JL (May 2010). "Intracellular compartmentation of CTP synthase in Drosophila". Journal of Genetics and Genomics = Yi Chuan ... These include bacteria (C. crescentus), yeast (S. cerevisiae), fruit flies (D. melanogaster) and human cells. These filamentous ... cell model of leukemia though such mutations were not found in human patients with ara-C resistance. Pyrimidine biosynthesis ... Nature Cell Biology. 12 (8): 739-46. doi:10.1038/ncb2087. PMC 3210567. PMID 20639870. Noree C, Sato BK, Broyer RM, Wilhelm JE ( ...
Rijksen G, Staal GE, Beks PJ, Streefkerk M, Akkerman JW (December 1982). "Compartmentation of hexokinase in human blood cells. ... Cytogenetics and Cell Genetics. 74 (3): 187-8. doi:10.1159/000134409. PMID 8941369. "Entrez Gene: HK3 hexokinase 3 (white cell ... Within cells, HK3 localizes to the cytoplasm and putatively binds the perinuclear envelope. HK3 is the predominant hexokinase ... provided by RefSeq, Apr 2009] HK3 is one of four highly homologous hexokinase isoforms in mammalian cells. This protein has a ...
Media related to Cell compartmentation at Wikimedia Commons v t e. ... Cellular compartments in cell biology comprise all of the closed parts within the cytosol of a eukaryotic cell, usually ... It was thought that compartmentalization is not found in prokaryotic cells., but the discovery of carboxysomes and many other ... Other compartments such as peroxisomes, lysosomes, the endoplasmic reticulum, the cell nucleus or the Golgi apparatus are not ...
Spatial compartmentation of transcriptional regulation and cell-cell signaling are examples. The vertebrate embryo is organized ... An example of compartmentation is found in the developing spine: all vertebrae contain bone-forming cells, but those in the ... cell-cell signaling pathways, cell adhesion processes, anteroposterior axis formation Additional core processes, such as ... Other forms of regulatory compartmentation include different cell types, developmental stages, and sexes. Gerhart and Kirschner ...
... cells, biological samples, cell villi) without deformation. SICM is used in an electrolyte-containing solution, so can be used ... "Beta2-adrenergic receptor redistribution in heart failure changes cAMP compartmentation". Science. 327 (5973): 1653-1657. doi: ... clamping the pipette by suction to the surface of a cell and then monitoring the activity of the sodium channels in the cell ... "The use of scanning ion conductance microscopy to image A6 cells". Mol. Cell Endocrinol. 217 (1-2): 101-108. doi:10.1016/j.mce. ...
... activity in Schwann cells: immunofluorescence localization and compartmentation of mu- and mCANP". J. Neurosci. Res. 29 (3): ... Design of an antisense strategy for calpain depletion in cultured cells". J. Biol. Chem. 271 (31): 18825-30. doi:10.1074/jbc. ... Cytogenet Cell Genet. 53 (4): 225-9. doi:10.1159/000132937. PMID 2209092. "Entrez Gene: CAPN1 calpain 1, (mu/I) large subunit ... produce necrotic but not apoptotic cell death in human CHP100 neuroblastoma cultures via a mechanism involving calpain". ...
... activity in Schwann cells: immunofluorescence localization and compartmentation of mu- and mCANP". Journal of Neuroscience ... "Capn4 mRNA level is correlated with tumour progression and clinical outcome in clear cell renal cell carcinoma". The Journal of ... Design of an antisense strategy for calpain depletion in cultured cells". The Journal of Biological Chemistry. 271 (31): 18825- ... Tidball JG, Spencer MJ (January 2000). "Calpains and muscular dystrophies". The International Journal of Biochemistry & Cell ...
However, NMNAT1 is dispensable for cell viability, as homozygous deletion of this gene occurs in glioblastoma tumors and cell ... Berger F, Lau C, Dahlmann M, Ziegler M (2006). "Subcellular compartmentation and differential catalytic properties of the three ... doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. Berger F, Lau C, Ziegler M (2007). "Regulation of poly(ADP-ribose ... 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635-48. ...
Compartmentation of photosynthesis in cells and tissues of C(4) plants.. „J Exp Bot". 356 (52), s. 577-90, 2001. PMID: 11373306 ... Intracellular localization of phosphoenolopyruvate carboxykinase in bundle sheath cells of C4 plants. Plant Cell Physiol. 25, ... Plant Cell Physiol.". 33, s. 333-342, 1992. *↑ a b c Rowan F. Sage. The evolution of C4photosynthesis. „New Phytologist". 161 ( ... Plant Cell Physiol. 48, 325-330.. *↑ Martin F., Winspear M.J., MacFarlane J.D. Oaks A. 1983. Effect of methionine sulfoximine ...
"The candidate phylum Poribacteria by single-cell genomics: new insights into phylogeny, cell-compartmentation, eukaryote-like ... "Shedding light on cell compartmentation in the candidate phylum Poribacteria by high resolution visualisation and ... Single-cell genomics and metagenomic shotgun sequencing approaches reveal a poribacterial genome size range between about 4.2 ... Cell compartmentalization into distinct membrane-bound organelles is a universal and defining property of eukaryotes, but had ...
Damage in the skin tissue of potato tuber causes a disruption of cell compartmentation, resulting in browning. The brown or ... Sap burn is therefore the initiating event of PPO in mango skin, as it breaks down cell compartments. PPO is located in mango ... Because the substrates of these PPO reactions are located in the vacuoles of plant cells damaged mainly by improper harvesting ... Catechol oxidase-type PPO is located in the chloroplasts of mango skin cells and its phenolic substrates in the vacuoles. ...
Prokaryotic cells are usually much smaller than eukaryotic cells. Therefore, prokaryotes have a larger surface-area-to-volume ... Fuerst JA (2005). "Intracellular compartmentation in planctomycetes". Annual Review of Microbiology. 59: 299-328. doi:10.1146/ ... The distinction is that eukaryotic cells have a "true" nucleus containing their DNA, whereas prokaryotic cells do not have a ... Also, as with multicellular eukaryotes, these changes in expression often appear to result from cell-to-cell signaling, a ...
The cell wall plays a very important role in tolerance and Cd detoxification in the mining ecotype S. alfredii. Common mining ... Yang, X., T. Li, J. Yang, Z. He, L. Lu, F. Meng (2006). Zinc compartmentation in root, transport into xylem, and absorption ... Vacuole, nonchlorophyllous mesophyll and other water storage cell types may be locations in S. alfredii which hyperaccumulate ... into leaf cells in the hyperaccumulating species of Sedum alfredii Hance. Planta, 224, 185-195. Yang, X. E., X. X. Long, H. B. ...
... other cell types have followed suit, as exemplified by the pancreas (acinar and beta cells), T-cells, and smooth muscle. Levels ... The NAADP/TPC axis has been reported to exhibit such signal compartmentation, such local Ca2+ signalling, in different ... "Cell-permeant NAADP: a novel chemical tool enabling the study of Ca2+ signalling in intact cells". Cell Calcium. 43 (6): 531-8 ... For example, NAADP/TPCs are unique drivers of cell killing by Cytotoxic T cells. Similarly, Phagocytosis via the Fc receptor in ...
... is critical to cell survival. Nearly every eukaryotic cell, from plants to yeast to humans, expresses a form of the GCL protein ... implications for the compartmentation of glutathione biosynthesis in the Brassicaceae". The Plant Journal. 41 (1): 15-30. doi: ... In the majority of cells and tissues, the expression of GCLM protein is lower than GCLC and GCLM is therefore limiting in the ... However, in cancer, GCL expression and activity is enhanced, which serves to both support the high level of cell proliferation ...
Fuerst JA (2005). "Intracellular compartmentation in planctomycetes". Review. Annual Review of Microbiology. 59: 299-328. doi: ... The cell nucleus contains all of the cell's genome, except for the small amount of mitochondrial DNA and, in plant cells, ... At a certain point during the cell cycle in open mitosis, the cell divides to form two cells. In order for this process to be ... Other multinucleate cells in the human are osteoclasts a type of bone cell. Multinucleated and binucleated cells can also be ...
Fuerst J (2005). "Intracellular compartmentation in planctomycetes". Annu Rev Microbiol. 59: 299-328. doi:10.1146/annurev.micro ... Comparison of a typical bacterial cell and a typical human cell (assuming both cells are spheres) : The cell envelope is ... while having no effects on human cells which have no cell wall, only a cell membrane. There are two main types of bacterial ... the bacterial cell wall provides structural integrity to the cell. In prokaryotes, the primary function of the cell wall is to ...
Examples of these processes include signal transduction from the cell membrane to sites within the cell, such as the cell ... Aw, T.Y. (2000). "Intracellular compartmentation of organelles and gradients of low molecular weight species". Int Rev Cytol. ... cell signaling, and the generation of action potentials in excitable cells such as endocrine, nerve and muscle cells. The ... without damaging the other cell membranes, only about one quarter of cell protein was released. These cells were also able to ...
Compartmentation of energy metabolism in atrial myocardium of patients undergoing cardiac surgery (kaasautor). // Mol. Cell. ... Lack of dystrofin is associated with altered integration of the mitochondria and ATPases in slow-twitch muscle cells of MDX ... Structure-function relationship in regulation of the energy transfere between mitochondria and ATPases in cardiac cell ( ...
A cell wall that contains chitin Less compartmentation between cells; the hyphae of higher fungi have porous partitions called ... pores in the cell wall that link adjacent cells and allow plant cells to communicate with adjacent cells. Animals have a ... The cells of plants and algae, fungi and most chromalveolates have a cell wall, a layer outside the cell membrane, providing ... The cell wall also prevents over-expansion when water enters the cell. The major polysaccharides making up the primary cell ...
... and Cell/Stem Cell Therapy. Regenerative Medicine, Artificial Cells and Nanomedicine. 1. Hackensack, NJ: World Scientific. ISBN ... The naturally arising, three-dimensional compartmentation observed within fossilized seepage-site metal sulphide precipitates ... The data suggest that viruses originated from ancient cells that co-existed with the ancestors of modern cells. These ancient ... Only when true cells had evolved would they gradually adapt to saltier environments and enter the ocean. Another protocell ...
... micro-compartmentation and metabolite channeling by multi-enzyme complexes.,. The mechanisms of the cell enhancing and neuro- ... Hans M. Eppenberger at the Institute of Cell Biology at ETH Zurich with distinction and received the ETH prize and medal. From ... Wallimann's main areas of interest are: The structure, function, subcellular compartmentation and molecular physiology of ... Wallimann was awarded the title of Professor and in the next two years he became Head and Deputy Head of the Institute of Cell ...
Controlling cell volumeEdit. Failure of the Na⁺-K⁺ pumps can result in swelling of the cell. A cell's osmolarity is the sum of ... Lynch RM, Paul RJ (March 1987). "Compartmentation of carbohydrate metabolism in vascular smooth muscle". The American Journal ... When this is higher than the osmolarity outside of the cell, water flows into the cell through osmosis. This can cause the cell ... In fact, all cells expend a large fraction of the ATP they produce (typically 30% and up to 70% in nerve cells) to maintain ...
In tissues and cells that consume ATP rapidly, especially skeletal muscle, but also brain, photoreceptor cells of the retina, ... Wallimann T, Wyss M, Brdiczka D, Nicolay K, Eppenberger HM (January 1992). "Intracellular compartmentation, structure and ... In the cells, the "cytosolic" CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There ... The bound cytosolic CK accepts the PCr shuttled through the cell and uses ADP to regenerate ATP, which can then be used as ...
Gitai Z (2005). "The new bacterial cell biology: moving parts and subcellular architecture". Cell 120 (5): 577-86. doi:10.1016/ ... Fuerst J (2005). "Intracellular compartmentation in planctomycetes". Annu Rev Microbiol 59: 299-328. doi:10.1146/annurev.micro. ... Gitai Z (March 2005). "The new bacterial cell biology: moving parts and subcellular architecture". Cell 120 (5): 577-86. doi: ... Cell-Cell Communication in Bacteria on-line lecture by Bonnie Bassler, and TED: Discovering bacteria's amazing communication ...
Fuerst JA (2005). "Intracellular compartmentation in planctomycetes". Annual Review of Microbiology. 59: 299-328. doi:10.1146/ ... Anucleated cells. Human red blood cells, like those of other mammals, lack nuclei. This occurs as a normal part of the cells' ... At a certain point during the cell cycle in open mitosis, the cell divides to form two cells. In order for this process to be ... Other multinucleate cells in the human are osteoclasts a type of bone cell. Multinucleated and binucleated cells can also be ...
While most cell biologists consider the term organelle to be synonymous with cell compartment, a space often bound by one or ... Fuerst JA (October 13, 2005). "Intracellular compartmentation in planctomycetes". Annual Review of Microbiology. 59: 299-328. ... In cell biology, an organelle is a specialized subunit, usually within a cell, that has a specific function. The name organelle ... Not all eukaryotic cells have each of the organelles listed below. Exceptional organisms have cells that do not include some ...
The sessile mother cell produces a free-swimming daughter cell. The daughter cell must then attach to a surface before starting ... Fuerst JA (October 2005). "Intracellular compartmentation in planctomycetes". Annual Review of Microbiology. 59 (1): 299-328. ... and axial symmetric cells. During cell division in members of P. limnophila, the daughter cells originate from the region ... During cell division in Fuerstia marisgermanicae, a tubular structure is connected from the bud to the mother cell. The species ...
Görlich, Dirk (1999). "Transport between the cell nucleus and the cytoplasm". Ann. Rev. Cell Dev. Biol. (15): 607-660. doi: ... 2005). "Intracellular compartmentation in planctomycetes". Annu Rev Microbiol. 59: 299-328. doi:10.1146/annurev.micro.59.030804 ... Görlich, Dirk; Kutay, U (1999). "Transport between the cell nucleus and the cytoplasm". Ann. Rev. Cell Dev. Biol. 15 (15): 607- ... Goldring, SR (1987). "Human giant cell tumors of bone identification and characterization of cell types". J Clin Invest (79(2 ...
Wallimann T, Hemmer W (1994). "Creatine kinase in non-muscle tissues and cells". Molecular and Cellular Biochemistry. 133-134 ( ... "Intracellular compartmentation, structure and function of creatine kinase isoenzymes in tissues with high and fluctuating ...
Cell Motility and the Cytoskeleton. 2009, 66 (12): 1100-5. PMID 19746415. doi:10.1002/cm.20417.. ... resolution analysis of gut environment and bacterial microbiota reveals functional compartmentation of the gut in wood‐feeding ...
Seminars in Cell & Developmental Biology, roč. 30, s. 36 - 44. Dostupné online [cit. 2018-04-21]. DOI: 10.1016/j.semcdb.2014.02 ... DYM, M.; FAWCETT, D. W.. The blood-testis barrier in the rat and the physiological compartmentation of the seminiferous ... The Sertoli cell: Novel clinical potentiality. HORMONES, roč. 14, čís. 4, s. 504 - 514. Dostupné online [cit. 2018-04-21]. DOI ... Mechanism of Humoral and Cellular Immune Modulation Provided by Porcine Sertoli Cells. Journal of Korean Medical Science, 2008- ...
Nutrient-Sensitive Mitochondrial NAD+ Levels Dictate Cell Survival». Cell, 130, 2007, pàg. 1095-107. DOI: 10.1016/j.cell. ... Stoichiometry and compartmentation of NADH metabolism in Saccharomyces cerevisiae». FEMS Microbiol. Rev., 25, 1, 2001, pàg. 15- ... 50,0 50,1 Diefenbach J, Bürkle A «Introduction to poly(ADP-ribose) metabolism». Cell. Mol. Life Sci., 62, 7-8, 2005, pàg. 721- ... Characterization of NAD Uptake in Mammalian Cells». J. Biol. Chem., 283, 10, 2008, pàg. 6367-74. DOI: 10.1074/jbc.M706204200. ...
2007). "Nutrient-Sensitive Mitochondrial NAD+ Levels Dictate Cell Survival". Cell 130 (6): 1095-107. PMID 17889652. doi:10.1016 ... 2001). "Stoichiometry and compartmentation of NADH metabolism in Saccharomyces cerevisiae". FEMS Microbiol. Rev. 25 (1): 15-37 ... "Plant Cell 18 (3): 688-98. PMC 1383643. PMID 16461578. doi:10.1105/tpc.105.039354.. ... doi:10.1016/j.cell.2007.03.024.. *↑ Blinova K, Carroll S, Bose S; et al. (2005). "Distribution of mitochondrial NADH ...
Clapham DE (2007). "Calcium signaling". Cell. 131 (6): 1047-58. doi:10.1016/j.cell.2007.11.028. PMID 18083096.. ... Compartmentation and communication in living systems. Ligand conduction: a general catalytic principle in chemical, osmotic and ... Sauke DJ, Metzler DE, Metzler CM (2001). Biochemistry: the chemical reactions of living cells (2nd ed.). San Diego: Harcourt/ ... Mendel RR, Bittner F (2006). "Cell biology of molybdenum". Biochim. Biophys. Acta. 1763 (7): 621-35. doi:10.1016/j.bbamcr. ...
A cell wall that contains chitin. *Less compartmentation between cells; the hyphae of higher fungi have porous partitions ... Cell wallEdit. Main article: Cell wall. The cells of plants and algae, fungi and most chromalveolates have a cell wall, a layer ... Plant cellEdit. Main article: Plant cell. Plant cells are quite different from the cells of the other eukaryotic organisms. ... The plasmodesmata, pores in the cell wall that link adjacent cells and allow plant cells to communicate with adjacent cells.[31 ...
"Cell. 130 (6): 1095-107. doi:10.1016/j.cell.2007.07.035. PMC 3366687. PMID 17889652.. ... Koch-Nolte F, Fischer S, Haag F, Ziegler M (2011). "Compartmentation of NAD+-dependent signalling". FEBS Lett. 585 (11): 1651-6 ... "Cell. 155 (7): 1624-1638. doi:10.1016/j.cell.2013.11.037. PMC 4076149. PMID 24360282.. ... In recent years, NAD+ has also been recognized as an extracellular signaling molecule involved in cell-to-cell communication.[ ...
... they press against the cell walls of the phytobiont's root cells. Often the fungal and plant cell walls become almost ... doi:10.1111/j.1574-6968.2005.00044.x. Lasat, M.M.; Baker, A.J.M.; Kochian, L.V. (1998). "Altered Zn compartmentation in the ... Upon the connection of fungal hyphae and root cap cells, growth must continue inwards to the epidermal cells wherein the hyphae ... Although EcM fungal hyphae form the Hartig net outside of root cells, penetration of plant cortical cells occasionally occurs. ...
Palisade mesophyll cells can contain 30-70 chloroplasts per cell, while stomatal guard cells contain only around 8-15 per cell ... "Chloroplast proteomics highlights the subcellular compartmentation of lipid metabolism". Progress in Lipid Research. 49 (2): ... This event is called endosymbiosis, or "cell living inside another cell with a mutual benefit for both". The external cell is ... Chloroplasts cannot be made by the plant cell and must be inherited by each daughter cell during cell division. ...
doi:10.1016/j.cell.2016.12.039. PMC 5329766. PMID 28187287. Cooper GM (2000). "The Molecular Composition of Cells". The Cell: A ... Compartmentation and communication in living systems. Ligand conduction: a general catalytic principle in chemical, osmotic and ... Proteins are also important in cell signaling, immune responses, cell adhesion, active transport across membranes, and the cell ... Binding of the hormone to insulin receptors on cells then activates a cascade of protein kinases that cause the cells to take ...
... termination of the operational cells is rather obvious. If there are support cells in the operational area, they may be ... Suvorov emphasizes that the process of forming new illegal residencies was the Soviet doctrine for imposing compartmentation. ... Cell systems are evolving to more decentralized models, sometimes because they are enabled by new forms of electronic ... Agents also may operate in networks, for which the classic security structure is the cell system. The agent may join a ...
Once the molecule is ingested it accumulates in muscle cells where it can be phosphorylated, increasing the pool of ... Compartmentation of Creatine Phosphokinase in Muscle". Regulation of Phosphate and Mineral Metabolism. Advances in Experimental ... it will convert ATP into PCr which will then move back out into the cells cytoplasm to be converted into ATP (by cytoplasmic ... Compartmentation of Creatine Phosphokinase in Muscle", Regulation of Phosphate and Mineral Metabolism, Advances in Experimental ...
... solution in the photosynthesis cells. The sucrose is actively transported to the companion cells of the smallest veins in the ... Recent Advances Phloem Transport and Assimilate Compartmentation. Ho, Y.K. (Manhattan, 2004.)Advanced Level Biology for Hong ... Cells in a sugar source "load" a sieve-tube element by actively transporting solute molecules into it. This causes water to ... In sugar sinks, cells actively transport solutes out of the sieve-tube elements, producing the exactly opposite effect. The ...
Studies carried out in his laboratory show that these sterols are structuring agents of plant cell membranes and in particular ... Regulation of sterol content in membranes by subcellular compartmentation of steryl esters accumulating in a sterol- ... cell division, auxin transport, membrane flux and reflux, secretion of suberin, a major constituent of epidermis, and leaf ...
Upon low frequency stimulation of knockout cells, these molecules are likely to diffuse out of the cell before a concentration ... Developmentally regulated changes in cellular compartmentation and synaptic distribution of actin in hippocampal neurons. J. ... Cell 107:605-16 Fischer M, Kaech S, Knutti D, Matus A. 1998. Rapid actin-based plasticity in dendritic spines. Neuron 20:847-54 ... In the LTD environment, spine volume is decreased, cell communication is decreased, and there is a far greater ratio of G-actin ...
Gilley J, Ribchester RR, Coleman MP (October 2017). "S, Confers Lifelong Rescue in a Mouse Model of Severe Axonopathy". Cell ... Berger F, Lau C, Dahlmann M, Ziegler M (2006). "Subcellular compartmentation and differential catalytic properties of the three ... Rajman L, Chwalek K, Sinclair DA (2018). "Therapeutic Potential of NAD-Boosting Molecules: The In Vivo Evidence". Cell ... Synthesis and Functions in Mammalian Cells". Trends in Biochemical Sciences. 45 (10): 858-873. doi:10.1016/j.tibs.2020.05.010. ...
The plasma membrane of mammalian cells contains detergent-resistant membrane rafts enriched in glycosphingolipids and ... we show that T cell activation leads to a striking compartmentation in the rafts of activated T cell receptor and associated ... Membrane compartmentation is required for efficient T cell activation Immunity. 1998 Jun;8(6):723-32. doi: 10.1016/s1074-7613( ... The plasma membrane of mammalian cells contains detergent-resistant membrane rafts enriched in glycosphingolipids and ...
RGC signal inputs are collected by dendrites and output is distributed from the cell body via very thin (0.5-1 μm) and long (∼ ... Retinal ganglion cells (RGCs) are specialized projection neurons that relay an immense amount of visual information from the ... Retinal ganglion cells: Energetics, compartmentation, axonal transport, cytoskeletons and vulnerability Prog Retin Eye Res. ... The RGC cell body is larger than other retinal neurons, but is still only a very small fraction (one ten thousandths) of the ...
Transport and compartmentation of phosphite in higher plant cells - kinetic and 31P nuclear magnetic resonance studies. ... Transport and compartmentation of phosphite in higher plant cells - kinetic and 31P nuclear magnetic resonance studies. Plant, ... Lai F., Thacker J., Li Y.Y. & Doerner P. (2007) Cell division activity determines the magnitude of phosphate starvation ... Roby C., Martin J.B., Bligny R. & Douce R. (1987) Biochemical changes during sucrose deprivation in higher plant cells: ...
Cell Compartmentation*. Cells, Cultured. Cytoplasm / metabolism. Kinetics. Magnetic Resonance Spectroscopy / methods. ... Title: Plant, cell & environment Volume: 31 ISSN: 1365-3040 ISO Abbreviation: Plant Cell Environ. Publication Date: 2008 Oct ... 8877096 - Cell-substratum interactions mediate oncogene-induced phenotype of lung cancer cells.. ... 8928746 - Characterization of cell volume loss in cem-c7a cells during dexamethasone-induced apop.... 3604176 - The effects of ...
e, epidermal cells; la, laticifer cells; pt, petals; sp, sepals. Arrowheads point to laticifer cells in stem. Bars in (A) to (L ... cl, cross-connecting laticifer cells; le, lower epidermis; pm, palisade mesophyll cells; sm, spongy mesophyll cells; t, ... 1989). Cell and tissue cultures of Catharanthus roseus (L.) G. Don: A literature survey. Plant Cell Tissue Organ Cult. 18, 231- ... Your Name) has sent you a message from Plant Cell Message Body (Your Name) thought you would like to see the Plant Cell web ...
Zinc binding inside leaf cell organelles, Environmental Pollution" on DeepDyve, the largest online rental service for scholarly ... "Compartmentation of metals in foliage of Populus tremula grown on soils with mixed contamination. II. ... Zinc binding inside leaf cell organelles. Compartmentation of metals in foliage of Populus tremula grown on soils with mixed ... Compartmentation of metals in foliage of Populus tremulagrown on soils with mixed contamination. I. From the tree crown to leaf ...
Hsp25-immunopositive Purkinje cells are first seen at birth, when four clusters are visible in the vermis of lobules IV/V, and ... By postnatal day 2/3, six narrow parasagittal stripes of Hsp25-immunopositive Purkinje cells are seen in the vermis of the ... In the posterior lobules, most Purkinje cells in the vermis of lobules VIII and IX express Hsp25. This initial limited ... Furthermore, visual deprivation had no effect on the development of Hsp25 expression in Purkinje cells, suggesting that ...
Intracellular and cell-to-apoplast compartmentation of carbohydrate metabolism. Author:. Fettke, Joerg, Fernie, Alisdair R.. ...
Leach, R P, Rogers, J, Wheeler, K P, Flowers, T J and Yeo, A R (1990) Molecular Markers for Ion Compartmentation in Cells of ... Molecular Markers for Ion Compartmentation in Cells of Higher Plants 1. Isolation of vacuoles of high-purity ... The tonoplast plays a crucial role in ion compartmentation, which is a central feature of the salt tolerance of halophytes, but ...
DESCRIBE THE STRUCTURAL COMPARTMENTATION OF MAMMALIAN CELLS AND THE DIFFERING FUNCTIONS OF THESE COMPARTMENTS All mammalian ... Stem Cell Term Paper 1867 words , 8 pages English Section 1 Stem Cell Research Term Paper Stem Cells are undifferentiated cells ... Cells are the smallest units of life • All cells come from preexisting cells • TOK: cell theory replaces the former ideas of ... Education Index Describe the structural compartmentation of mammalian cells Describe the structural compartmentation of ...
Compartmentation of cholesterol within the cell PDF Only. Compartmentation of cholesterol within the cell. Liscum, Laura; Faust ...
... cells and tissue facts using a simple interactive process (flashcard, matching, or multiple choice). Finally a format that ... T: Explain cell volume - O: Doesnt explain what happens to cell. Tonicity: Cell swells, what happens to solution?. Solution is ... Tonicity: Cell shrinks, what happens to solution?. Solution is hypertonic. What crosses cell membranes, depend on? (2). Cell ... versions of PHYSIOLOGY - Compartmentation; cells and tissue: main , yours , all. Please enable JavaScript in your browser ...
Cell physiology 2015-4-10 The Golgi apparatus regulates cGMP-dependent protein kinase I compartmentation and proteolysis.. [ ... cGMP-dependent protein kinase I (PKGI) is an important effector of cGMP signaling that regulates vascular smooth muscle cell ( ... PKGI has been detected in the perinuclear region of cells, and recent data indicate that proprotein convertases (PCs) typically ... However, the role of the endomembrane system in PKGI compartmentation and processing is unknown. Here, we demonstrate that PKGI ...
Posterior hindgut.Microbial cell counts decrease by 2 orders of magnitude and cell density drops 50-fold between the P3 and P4 ... Microbial cell counts.Twenty gut sections each of N. corniger were homogenized in 0.5 ml phosphate-buffered saline (PBS) (49) ... The area of the circles reflects the microbial cell counts in the respective gut sections (Table 1). For definitions of the ... The microbiota of the crop consisted mostly of cocci, whereas the midgut microbiota was dominated by short rods; cells with a ...
Compartmentation of nitrate in barley root cells growing at different nitrogen levels. Abstracts AFRC Meeting on Plant and Soil ... Compartmentation of nitrate in root cells. Abstracts Society for Experimental Biology Plant Transport Group Discussion Meeting ... Compartmentation of nitrate in barley root cells. Abstracts 3rd International Symposium on Inorganic Nitrogen Assimilation, ... Nutrient compartmentation in cells and its relevance to the nutrition of the whole plant. in: Lawlor, D. W. and Porter, J. R. ( ...
The compartmentation of the model is more extensive than that of the AraGEM model (de Oliveira DalMolin et al., 2010) but less ... In plant cells, the NAD(H) pool is more oxidized than the NADP(H) pool in both the light and the dark (Heineke et al., 1991; ... Penning de Vries, F.W.T. (1975) The cost of maintenance processes in plant cells. Ann. Bot., 39, 77-92.. *CAS ... Rontein, D., Dieuaide-Noubhani, M., Dufourc, E.J., Raymond, P. and Rolin, D. (2002) The metabolic architecture of plant cells. ...
... subcellular compartmentation; technical advance; temperature stress; transport ... This leads to a method for predicting cell maintenance costs on the basis of the measured flux ratio between the oxidative ... Arabidopsis thaliana; cell maintenance; flux balance analysis; genome-scale metabolic model; hyper-osmotic stress; metabolic ... A method for accounting for maintenance costs in flux balance analysis improves the prediction of plant cell metabolic ...
CNS Nervous System PET brain cell enzyme genes neurochemistry neuroscience positron emission tomography (PET) protein ...
These cells showed halophytic growth responses comparable to those of the whole plant. In total, 97 up-regulated proteins and ... These cells showed halophytic growth responses comparable to those of the whole plant. In total, 97 up-regulated proteins and ... cell growth, and cytoskeleton metabolism. Effective regulatory protein expression related to energy, stress defense, and ... cell growth, and cytoskeleton metabolism. Effective regulatory protein expression related to energy, stress defense, and ...
Chloroplast Lipid cell cell wall chemistry endoplasmic reticulum enzyme growth membrane metabolism mitochondria plant plasma ... Protoplasts - for Compartmentation Studies S. S. Thayer. Pages 54-65 * The Marker Concept in Cell Fractionation ...
In vascular smooth muscle cells and HEK-293 cells, AC1 selectively slowed cell proliferation while AC2, AC5, and AC6 had little ... in HASM cells (Gros et al., 2006; Bogard et al., 2012). AC2 overexpressed in the same cells is unable to mediate the ... Recent efforts using RNA sequencing to define the full range of GPCR expressed in a given cell type reveal that most cells ... 1996) cAMP compartmentation is responsible for a local activation of cardiac Ca2+ channels by β-adrenergic agonists. Proc Natl ...
Cell compartmentation and metabolic channeling . Jena, Amsterdam: Fischer Verlag / Elsevier/North Holland Biomedical, 471-484. ... J.Cell Sci. 287-300. Heslop-Harrison J, Heslop-Harrison Y. 1985 . Germination of stress-tolerant Eucalyptus pollen. J.Cell Sci ... Cytoskeletal elements, cell shaping and movement in the angiosperm pollen tube. J.Cell Sci. 91 : 49-60. Heslop-Harrison J. 1988 ... SEB Symposium 32: Cell-Cell Recognition . Cambridge: Cambridge University Press, 121-136. Heslop-Harrison J. 1978 . Genetics ...
... partly because of a lack of tools with which to investigate these processes in living cells. We have previously reported the ... as a genetic tool for manipulation of the NAD+/NADH ratio in human cells. Here, we present triphosphopyridine nucleotide ... Sies, H. Metabolic compartmentation (Academic Press, 1982).. *. 4. Klingenberg, M. & Buecher, T. Biological oxidations. Annu. ... Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells. Mol. Cell 55, 253-263 (2014). ...
Compartmentation of cholesterol within the cell.. Liscum L, Faust JR.. Curr Opin Lipidol. 1994 Jun;5(3):221-6. Review. ... Analysis of a Chinese hamster ovary cell mutant with defective mobilization of cholesterol from the plasma membrane to the ... Characterization of Chinese hamster ovary cells that are resistant to 3-beta-[2-(diethylamino)ethoxy]androst-5-en-17-one ... Biochim Biophys Acta Mol Cell Res. 2019 Dec;1866(12):118552. doi: 10.1016/j.bbamcr.2019.118552. Epub 2019 Sep 2. ...
Leach, R P, Wheeler, K P, Flowers, T J and Yeo, A R (1990) Molecular Markers for Ion Compartmentation in Cells of Higher Plants ... The relevance of the lipid composition of the tonoplast to its central role in ion compartmentation within the halophyte cell ... Molecular Markers for Ion Compartmentation in Cells of Higher Plants II. LIPID COMPOSITION OF THE TONOPLAST OF THE HALOPHYTE ...
... and 10-formyltetrahydrofolate relative to intact cells or the particulate fraction. Exposure of cells to 10 μM trimetrexate for ... and 10-formyltetrahydrofolate relative to intact cells or the particulate fraction. Exposure of cells to 10 μM trimetrexate for ... and 10-formyltetrahydrofolate relative to intact cells or the particulate fraction. Exposure of cells to 10 μM trimetrexate for ... and 10-formyltetrahydrofolate relative to intact cells or the particulate fraction. Exposure of cells to 10 μM trimetrexate for ...
I. Development and compartmentation of cerebellar cortex.. *Functional cloning of candidate genes that regulate purkinje cell- ... Unipolar brush cells in cerebellar cortex. 9. The unipolar brush cells of the mammalian cerebellum and cochlear nucleus: ... Characterization of purkinje cells in the goldfish cerebellum during eye movement and adaptive modification of the vestibulo- ... What features of visually guided arm movements are encoded in the simple spike discharge of cerebellar Purkinje cells. 27. Some ...
Contracture in isolated adult rat heart cells. Role of Ca^,2+,, ATP, and compartmentation HAWORTH RA. ... In the FOD diet group, the number of surviving rod-shaped cells after hypoxia and reoxygenation was smaller than that of the ... Modulation of dihydropyridine-sensitive calcium channels in heart cells by fish oil fatty acids HALLAQ H. ... Supplementation with EPA did not affect the number of rod-shaped cells, but attenuated reoxygenation-induced reduction in the ...
Short-term and long-term control mechanisms - regulation of enzyme activity, gene expression regulation; cell signaling. ... Compartmentation. Membrane transport. Biochemistry of hormones and the hormonal cascade system.. Aims ...
Fuerst JA (2005). "Intracellular compartmentation in planctomycetes". Review. Annual Review of Microbiology. 59: 299-328. doi: ... The cell nucleus contains all of the cells genome, except for the small amount of mitochondrial DNA and, in plant cells, ... At a certain point during the cell cycle in open mitosis, the cell divides to form two cells. In order for this process to be ... Other multinucleate cells in the human are osteoclasts a type of bone cell. Multinucleated and binucleated cells can also be ...
  • On the other hand, the external Pi nutrition status and the metabolic state of the cells had a strong influence on the intracellular compartmentalization of xenobiotic Phi. (biomedsearch.com)
  • Compartmentation of intracellular folates. (elsevier.com)
  • Fingerprint Dive into the research topics of 'Compartmentation of intracellular folates. (elsevier.com)
  • Studies of transgenic creatine kinase and adenylate kinase deficient mice, along with pharmacological targeting of individual enzymes, have revealed the importance of near-equilibrium reactions in the dissipation of metabolite gradients and communication of energetic signals to distinct intracellular compartments, including the cell nucleus and membrane metabolic sensors. (biologists.org)
  • Potentially as the initial cell surface event for intracellular signaling, NaAsO 2 induced aggregation of GPI-anchored protein Thy-1 and superoxide production. (jimmunol.org)
  • In resting T cells, rafts as identified by GM1 staining have been found localized in intracellular compartments and are released to the cell surface with receptor ligation ( 25 ). (rupress.org)
  • We have shown previously that the ADP-ribosylation factor (ARF)-6 GTPase localizes to the plasma membrane and intracellular endosomal compartments.Expression of ARF6 mutants perturbs endosomal trafficking and the morphology of the peripheral membrane system.Furthermore, we have shown that the ARF6-containing intracellular compartment partially colocalized with transferrin receptors and cellubrevin and morphologically resembled the recycling endocytic compartment previously described in CHO cells. (nih.gov)
  • Here we demonstrate that at the lowest detectable levels of protein expression by cryoimmunogold electron microscopy, ARF6 localized predominantly to an intracellular compartment at the pericentriolar region of the cell. (nih.gov)
  • Furthermore, we have shown that the ARF6-containing intracellular compartment partially colocalized with transferrin receptors and cellubrevin and morphologically resembled the recycling endocytic compartment previously described in CHO cells. (nih.gov)
  • In addition, intracellular dye filling of electrophysiologically characterized progenitor cells in the VZ demonstrates that these cells have the morphology of radial glia. (jneurosci.org)
  • Publications] M.Nishimura: 'Molecular Approaches on Compartmentation and Metabolic Regulation Intracellular protein trafficking in oil seed. (nii.ac.jp)
  • The biological research of the Collaborative Research Centre (CRC 593) was focused on the topic of how eukaryotic cells generate, maintain and alter their intracellular compartmentation in both healthy and diseased states. (uni-marburg.de)
  • This included i) the transport of metals, metal complexes, nucleic acids, lipids and proteins to, within and out of various cell compartments such as mitochondria, ii) the assembly and activation of plasma membrane ion channels in the secretory pathway, and iii) the intracellular vesicle transport and protein sorting in (polarized) cells. (uni-marburg.de)
  • Several groups studied how these pathogens exploit and alter the existing intracellular compartmentation to ensure their own survival. (uni-marburg.de)
  • Intracellular compartmentation of arginases may have important implications for arginine metabolism in mammalian cells. (physiology.org)
  • The plasma membrane of mammalian cells contains detergent-resistant membrane rafts enriched in glycosphingolipids and cholesterol. (nih.gov)
  • The tonoplast plays a crucial role in ion compartmentation, which is a central feature of the salt tolerance of halophytes, but we do not know the properties of the membrane that confer this ability. (sussex.ac.uk)
  • The term eukaryotic refers to the cell having specific membrane bound organelles, which are not present in prokaryotic cells. (educationindex.com)
  • The defining feature of a eukaryotic cell is usually its membrane bound nucleus (the exception being the red blood cell) [1]. (educationindex.com)
  • Cell membrane model is known as? (memorize.com)
  • from Latin nucleus or nuculeus, meaning kernel or seed) is a membrane-bound organelle found in eukaryotic cells. (wikipedia.org)
  • At low surface area-to-volume ratios the diffusion of nutrients and waste products across the bacterial cell membrane limits the rate at which microbial metabolism can occur, making the cell less evolutionarily fit. (wikipedia.org)
  • Comparison of a typical bacterial cell and a typical human cell (assuming both cells are spheres) : The cell envelope is composed of the cell membrane and the cell wall. (wikipedia.org)
  • The bacterial cell wall differs from that of all other organisms by the presence of peptidoglycan which is located immediately outside of the cell membrane. (wikipedia.org)
  • Since the cell wall is required for bacterial survival, but is absent in some eukaryotes, several antibiotics (notably the penicillins and cephalosporins) stop bacterial infections by interfering with cell wall synthesis, while having no effects on human cells which have no cell wall, only a cell membrane. (wikipedia.org)
  • The main structures making up the nucleus are the nuclear envelope , a double membrane that encloses the entire organelle and isolates its contents from the cellular cytoplasm , and the nuclear matrix (which includes the nuclear lamina ), a network within the nucleus that adds mechanical support, much like the cytoskeleton , which supports the cell as a whole. (wikipedia.org)
  • [6] Eukaryotic cells also contain other membrane-bound organelles such as mitochondria and the Golgi apparatus , and in addition, some cells of plants and algae contain chloroplasts . (wikipedia.org)
  • However, experimental results available to date on RBC membrane fluctuations have provided only limited information on select regions of the cell membrane with limited spatial and/or temporal resolution ( 7 ⇓ - 9 ). (pnas.org)
  • Eukaryotic cells differ fundamentally from their prokaryotic counter-parts by their possession of internal, membrane-bound, compartments. (biologists.org)
  • These functions are only possible in eukaryotic cells because of the presence of the membrane-trafficking system. (biologists.org)
  • In the past 15 years, studies of yeast ( Saccharomyces cerevisiae ) and metazoan cells have revealed a common core of protein factors involved in transport carrier formation, compartment specificity and membrane fusion ( Bonifacino and Glick, 2004 ). (biologists.org)
  • 1989 ). Anion transport through the contraluminal cell membrane of renal proximal tubule. (biologists.org)
  • found that the activating receptor NKp46 and the inhibitory receptor Ly49A exhibited dynamic movements in the plasma membrane that differed between educated and hyporesponsive NK cells. (sciencemag.org)
  • Although both receptors were generally restricted to membrane microdomains, NKp46 molecules spent less time in an individual microdomain and diffused faster on educated cells compared to hyporesponsive cells. (sciencemag.org)
  • Either disrupting the actin cytoskeleton or adding cholesterol to the cells prohibited activating signaling, suggesting that the dynamics of receptor movements within the cell membrane are critical for the proper activation of NK cells. (sciencemag.org)
  • The main structural elements of the nucleus are the nuclear envelope, a double membrane that encloses the entire organelle and keeps its contents separated from the cellular cytoplasm , and the nuclear lamina, a meshwork within the nucleus that adds mechanical support much like the cytoskeleton supports the cell as a whole. (bionity.com)
  • In this study, we report NaAsO 2 -induced signal cascades from the cell surface to the nucleus of murine thymic T lymphocytes that involve membrane rafts as an initial signal transducer. (jimmunol.org)
  • These results suggest that a membrane raft integrity-dependent cell surface event is a prerequisite for NaAsO 2 -induced protein tyrosine kinase/c-Jun amino-terminal kinase activation, superoxide production, and downstream caspase activation. (jimmunol.org)
  • The same set of stimuli also induced relocation of endogenous PKCθ and IKKs to a GM1 ganglioside-enriched, detergent-insoluble membrane compartment in primary T cells. (jimmunol.org)
  • Confocal microscopy further demonstrated that exogenously expressed PKCθ and IKKβ colocalize in the membrane of CD3/CD28-costimulated Jurkat T cells. (jimmunol.org)
  • Taken together, these data show that the activation of PKCθ by the TCR and CD28 plays an important role in the assembly and activation of IKK complexes in the T cell membrane. (jimmunol.org)
  • In this context, ganglioside and cholesterol enriched membrane microdomains (rafts, GEMs) serve as centers of signaling in T cells. (rupress.org)
  • Although CD28 can promote TcR/raft colocalization, evidence is lacking on whether the surface expression of membrane rafts can be targeted by CTLA-4 in its modulation of T cell responses. (rupress.org)
  • In this study, we demonstrate that both CD28 and CTLA-4 profoundly alter the surface expression of membrane rafts during T cell activation. (rupress.org)
  • Consistent with this, CD28 increased the presence of the linker of activated T cells (LAT) in purified membrane rafts, while CTLA-4 coligation effectively blocked this increase. (rupress.org)
  • from Latin [ nucleus ] error: {{lang}}: text has italic markup ( help ) or [ nuculeus ] error: {{lang}}: text has italic markup ( help ) , "little nut" or kernel) is a membrane-enclosed organelle found in most eukaryotic cells . (wikidoc.org)
  • However, another study on the distribution of ARF6 in subcellular fractions of Chinese hamster ovary (CHO) cells suggested that ARF6 did not localize to endosomes labeled after 10 min of horseradish peroxidase (HRP) uptake, but instead was uniquely localized to the plasma membrane, and that its reported endosomal localization may have been a result of overexpression. (nih.gov)
  • HRP labeling in cells expressing ARF6(Q67L), a GTP-bound mutant of ARF6, was restricted to small peripheral vesicles, whereas the mutant protein was enriched on plasma membrane invaginations. (nih.gov)
  • Significantly, the mitochondrial membrane potential of α and β RNAi cells was decreased compared to wild-type cells, as detected by MitoTracker Red CMXRos fluorescence microscopy and flow cytometry. (asm.org)
  • The first rate-limiting step is the stimulus-induced mobilization of AA from cell membrane phosphoglycerides by the enzymes phospholipase (PL) A 2 (acting on phosphatidylcholine) and diacylglycerol (DAG) lipase (acting on phosphatidylinositol). (physiology.org)
  • In addition, a variably reduced cell membrane association of those differentiation-specific Cldns was observed, especially within the infiltrating epidermal structures. (biomedcentral.com)
  • In contrast, Cldn1 (which is normally expressed in all the living layers of the epidermis) remained restricted to the cell membrane throughout the tumorigenesis protocol. (biomedcentral.com)
  • Integrin-associated protein (CD47) is a multiply membrane spanning member of the immunoglobulin superfamily that regulates some adhesion-dependent cell functions through formation of a complex with αvβ3 integrin and trimeric G proteins. (rupress.org)
  • Real-time investigation of the membrane structure and function of living cells is of utmost importance, however techniques are limited. (springer.com)
  • Caveolae are omega-shaped invaginations of the plasma membrane that, compared with the rest of the outer cell membrane, have a larger amount of cholesterol and glycoprotein and greater concentrations of lipid-modified signaling molecules. (ahajournals.org)
  • Their main structural uses are as part of biological membranes both internal and external, such as the cell membrane, or as a source of energy. (wikipedia.org)
  • However, the biomass constraint alone is not sufficient to predict realistic fluxes in central heterotrophic metabolism of plant cells because of the major demand on the energy budget due to transport costs and cell maintenance. (wiley.com)
  • Effective regulatory protein expression related to energy, stress defense, and carbohydrate metabolism play important roles in the salt-tolerance of H. glomeratus suspension cell cultures. (frontiersin.org)
  • Ascorbate also has a potential to affect other aspects of cancer cell metabolism due to its ability to promote reduction of iron(III) to iron(II) in numerous cellular metalloenzymes. (mdpi.com)
  • Altered metabolism of cancer cells by vitamin C can be beneficial by itself and promote activity of specific drugs. (mdpi.com)
  • This review considers the importance of compartmentation in the regulation of carbohydrate metabolism in leaves. (scielo.br)
  • We also consider compartmentation of carbohydrate metabolism between different cell types, using mainly our studies on leaves of temperate C3 gramineae. (scielo.br)
  • Here we present evidence of significant differences in carbon metabolism between epidermis, mesophyll, bundle sheath and vasculature based upon both single-cell sampling and immunolocalisation. (scielo.br)
  • Arginine metabolism in mammalian cells. (physiology.org)
  • The word metabolism can also refer to the sum of all chemical reactions that occur in living organisms, including digestion and the transport of substances into and between different cells, in which case the above described set of reactions within the cells is called intermediary metabolism or intermediate metabolism. (wikipedia.org)
  • The metabolism of cancer cells is different from the metabolism of normal cells, and these differences can be used to find targets for therapeutic intervention in cancer. (wikipedia.org)
  • Amino acids also contribute to cellular energy metabolism by providing a carbon source for entry into the citric acid cycle (tricarboxylic acid cycle), especially when a primary source of energy, such as glucose, is scarce, or when cells undergo metabolic stress. (wikipedia.org)
  • The crude mitochondrial fraction contained 20.1% of the total folate pool and included 5-formyl-tetrahydrofolate, 10-formyltetrahydrofolate and tetrahydrofolate in proportions similar to intact cells. (elsevier.com)
  • Exposure of cells to 10 μM trimetrexate for 30 min produced ∼45% interconversion of tetrahydrofolate cofactors to dihydrofolate in the cytosolic fraction, a level much greater than that observed in whole cell extracts (25-30%), but had no effect on folate pools in the crude mitochondrial fraction. (elsevier.com)
  • The cell nucleus contains all of the cell's genome, except for the small amount of mitochondrial DNA and, in plant cells, plastid DNA. (wikipedia.org)
  • The cell nucleus contains all of the cell's genome , except for a small fraction of mitochondrial DNA , organized as multiple long linear DNA molecules in a complex with a large variety of proteins , such as histones , to form chromosomes . (wikipedia.org)
  • Cells with any of the gag mutations displayed aberrant mitochondrial morphology characterized by elongated, unbranched tubes and highly fenestrated structures. (nih.gov)
  • Additionally, each of the gag mutations prevented mitochondrial fragmentation caused by loss of the mitochondrial fusion factor, Fzo1p, or by treatment of cells with sodium azide. (nih.gov)
  • Cells lacking Fzo1p display fragmented mitochondria that readily lose mitochondrial DNA (Hermann et al. (nih.gov)
  • Mitochondrial compartmentation and channeling etc... could be selected by the organizer after propositions. (bio.net)
  • Arginase I (a cytosolic enzyme) is highly expressed in the liver and to a much lesser extent in a few other cell types, whereas expression of arginase II (a mitochondrial enzyme) is widespread ( 20 , 25 ). (physiology.org)
  • Plant and cell physiology. (rothamsted.ac.uk)
  • Lectures will emphasize the cell biology, anatomy, and physiology of vertebrate reproductive systems and present a comparative approach to understanding sex determination. (reed.edu)
  • and the nuclear matrix (which includes the nuclear lamina), a network within the nucleus that adds mechanical support, much like the cytoskeleton supports the cell as a whole. (wikipedia.org)
  • An exploration of our current understanding of the cytoskeleton and its role in cell migration, morphogenesis, and disease. (reed.edu)
  • Part I of this two-part article, described a mechanical model of cell structure based on tensegrity architecture that explains how the mechanical behavior of the cell emerges from physical interactions among the different molecular filament systems that form the cytoskeleton. (biologists.org)
  • Recent work shows that the cytoskeleton also orients much of the cell's metabolic and signal transduction machinery and that mechanical distortion of cells and the cytoskeleton through cell surface integrin receptors can profoundly affect cell behavior. (biologists.org)
  • Other proteins have structural or mechanical functions, such as those that form the cytoskeleton, a system of scaffolding that maintains the cell shape. (wikipedia.org)
  • The function of Cell membranes? (memorize.com)
  • What crosses cell membranes, depend on? (memorize.com)
  • Together, these membranes serve to separate the cell's genetic material from the rest of the cell contents, and allow the nucleus to maintain an environment distinct from the rest of the cell. (wikipedia.org)
  • The nucleus of a typical mammalian cell will have about 3000 to 4000 pores throughout its envelope, each of which contains an eightfold-symmetric ring-shaped structure at a position where the inner and outer membranes fuse. (wikipedia.org)
  • are organisms whose cells have a nucleus enclosed within membranes , unlike prokaryotes ( Bacteria and Archaea ). (wikipedia.org)
  • Furthermore, P is also present in phospholipids, a major constituent of cell membranes. (royalsocietypublishing.org)
  • Glycosphingolipid/cholesterol enriched microdomains (GEMs, Rafts, detergent-insoluble glycolipid-enriched domains [DIGs], detergent-resistant membranes [DRMs]) on the surface of cells act as platforms that compartmentalize key components involved in signaling ( 17 , 18 ). (rupress.org)
  • 3 The abscess cavity contains inflammatory cells with intact cell membranes and nuclei, necrotic tissue debris, proteinaceous exudates, bacterial metabolites, and cytosolic amino acids, with or without the brain abscess's causative organism. (ajnr.org)
  • Scanning ion conductance microscopy (SICM) is a kind of scanning probe microscopy (SPM) that provides images of nonconducting surfaces that are covered with electrolytes, for example plasma membranes of biological cells, composed of non-conducting lipids [1]. (springer.com)
  • Proteins are also important in cell signaling, immune responses, cell adhesion, active transport across membranes, and the cell cycle. (wikipedia.org)
  • Here, we demonstrate that PKGI colocalizes with endoplasmic reticulum (ER), ER-Golgi intermediate compartment, GA cisterna, and trans-Golgi network proteins in pulmonary artery SMC and cell lines. (sigmaaldrich.com)
  • However, our knowledge of how receptors, cAMP signaling enzymes, effectors, and other key proteins form specific signaling complexes to regulate specific cell responses is limited. (aspetjournals.org)
  • The multicomponent nature of these systems and the spatiotemporal dynamics involved as proteins interact and move within a cell make cAMP responses highly complex. (aspetjournals.org)
  • Mouse models carrying mutations for the nine Usher-associated genes have splayed stereocilia, and some show delayed maturation of ribbon synapses suggesting these proteins may play different roles in terminal differentiation of auditory hair cells. (biomedsearch.com)
  • About half of the dry mass of a bacterial cell consists of carbon, and also about half of it can be attributed to proteins. (wikipedia.org)
  • In prokaryotes, the primary function of the cell wall is to protect the cell from internal turgor pressure caused by the much higher concentrations of proteins, and other molecules inside the cell compared to its external environment. (wikipedia.org)
  • These proteins can also transport the oxidized form of vitamin C, dehydroascorbate, permitting its preferential uptake by cancer cells with the subsequent depletion of critical cellular reducers as a result of ascorbate formation. (mdpi.com)
  • Arsenite has also been shown to induce activation of mitogen-activated protein kinase (MAPK) 3 ( 2 ) family member proteins, some of which may play a key role in apoptosis induction in leukemia cells ( 10 ). (jimmunol.org)
  • Rafts are enriched with GPI-linked proteins, palmitoylated transmembrane adaptors such as LAT (linker for activation of T cells), and myristoylated kinases such as p56 lck and p59 fyn ( 19 - 22 ). (rupress.org)
  • In this context, a family of immune cell specific adaptor proteins have been identified in recent years ( 26 - 28 ). (rupress.org)
  • Amongst the junctional complexes, the role in tumor formation of specific tight junction (TJ) proteins essential for cell polarity and the formation and maintenance of heterogeneous permeability barriers is not well understood. (biomedcentral.com)
  • Here, we studied the kinetics and mechanics of signaling complex formation in live Jurkat leukemic T cells using signaling proteins fluorescently tagged with variants of enhanced GFP (EGFP). (rupress.org)
  • The main corticosterone compartment in rat blood is that specifically bound to plasma proteins, with smaller compartments bound to blood cells or free. (jove.com)
  • The number of mitochondrion in a cell differs dependent of independent cell function, for example a goblet cell will use lots of ATP in the secretion of mucus, and will there for have a large amount of mitochondria in the cell. (educationindex.com)
  • B, Cells with the fzo1 mutation (MYY2005) or the double mutants dnm1 fzo1 (MYY2007), gag2 fzo1 (MYY2009), or gag3 fzo1 (MYY2011) were plated on glycerol-medium and cultured for 3 d at 30°C. C, Wild-type (MYY1200), dnm1/gag1 (MYY2013), gag2 (MYY2029), and gag3 (MYY2031) cells with GFP-labeled mitochondria were treated with 0.5 mM sodium azide and examined immediately (top) or after 30 min (bottom) by fluorescence microscopy. (nih.gov)
  • Mitochondria in cell death 2. (bio.net)
  • The immunosuppressant 15-deoxyspergualin [correction of 1,5-deoxyspergualin] reveals commonality between preT and preB cell differentiation. (rupress.org)
  • In particular, gradual variations in this single physical control parameter (cell shape distortion) can switch cells between distinct gene programs (e.g. growth, differentiation and apoptosis), and this process can be viewed as a biological phase transition. (biologists.org)
  • Our data indicate that along with the changes in epidermal cell morphology and differentiation that occur during tumor formation, there is a dramatic change in Cldn distribution consistent with cell polarity and barrier selectivity changes. (biomedcentral.com)
  • Among topics considered are structure and function of plants and animals, relations of organisms to each other and to their environment, energy relations of organisms, integrative and coordinating mechanisms of organisms, cell biology principles, genetics, molecular biology, reproduction, development and growth, and the evidence for organic evolution. (reed.edu)
  • Chapter 2 IB Biology 2.1 Cell Theory 2.1.1 Outline the cell theory (2). (educationindex.com)
  • The major challenge in biology today is biocomplexity: the need to explain how cell and tissue behaviors emerge from collective interactions within complex molecular networks. (biologists.org)
  • Brief summaries of the results of the individual groups are found in a special issue of European Journal of Cell Biology published in 2015 ( https://www.sciencedirect.com/journal/european-journal-of-cell-biology/vol/94/issue/7 ). (uni-marburg.de)
  • 16. Reciprocal trophic interactions between climbing fibres and purkinje cells in the rat cerebellum. (elsevier.com)
  • Collectively, these findings establish the existence of a differential vesicular trafficking mechanism for specific Usher protein variants in mouse cochlear hair cells, with the apical variants playing a potential role in endosomal recycling and stereocilia development/maintenance, and the basolateral variants involved in vesicle docking and/or fusion through SNAP25-mediated interactions. (biomedsearch.com)
  • The faster and more dynamic movement of NKp46 in educated NK cells may facilitate a swifter response to interactions with target cells. (sciencemag.org)
  • Binding and cooperative interactions between two B cell-specific transcriptional coactivators. (rupress.org)
  • Rap1 plays a dominant role in the control of cell-cell and cell-matrix interactions by regulating the function of integrins and other adhesion molecules in various cell types. (genome.jp)
  • The challenge is to understand how complex cell and tissue behaviors emerge from collective interactions among multiple molecular components at the genomic and proteomic levels and to describe molecular processes as integrated, hierarchical systems rather than isolated parts. (biologists.org)
  • In this review we will describe RGCs as having four subcellular components, (1) Dendrites, (2) Cell body, (3) Non-myelinated axon, including intraocular and optic nerve head portions, and (4) Myelinated axon, including the intra-orbital and intracranial portions. (nih.gov)
  • In this paper we analysed uptake, subcellular distribution and metabolic effects of Phi in tobacco BY-2 cells using in vivo(31)P nuclear magnetic resonance ((31)P-NMR) spectroscopy. (biomedsearch.com)
  • These data indicate that subcellular compartmentation accounts, in part, for the failure to oxidize tetrahydrofolate cofactors to dihydrofolate in the presence of antifolate levels that abolish dihydrofolate reductase activity. (elsevier.com)
  • Schematic of typical animal cell, showing subcellular components. (wikidoc.org)
  • Using a fractionation scheme that preserves tyrosine phosphorylation, we show that T cell activation leads to a striking compartmentation in the rafts of activated T cell receptor and associated signal-transducing molecules. (nih.gov)
  • The structures called "synthetic cells", or "artificial cells", or "protocells" (sometimes with different nuances in meaning) are chemical or biochemical systems based on micro-compartments that enclose a set of reacting molecules, mimicking the cell structure and behavior. (mdpi.com)
  • Conversely, Ly49A molecules were overall more constrained and diffused more slowly on educated cells. (sciencemag.org)
  • Educated natural killer (NK) cells have inhibitory receptors specific for self major histocompatibility complex (MHC) class I molecules and kill cancer cells more efficiently than do NK cells that do not have such receptors (hyporesponsive NK cells). (sciencemag.org)
  • however, individual NKp46 molecules resided in these domains for shorter periods and diffused faster on the surface of educated, compared to hyporesponsive, NK cells. (sciencemag.org)
  • These rafts also include signaling molecules belonging to the src-family, LAT (linker for activation of T cells) and Ras ( 1 , 4 , 6 ). (jimmunol.org)
  • At the same time, the introduction of new techniques for manipulating and probing individual molecules and cells has revealed the importance of the physical nature of the biochemical world. (biologists.org)
  • The function of caveolae has been considered to be transport of cholesterol, tumor suppression, compartmentation of signaling molecules, regulation of signaling, apoptosis, and transport of extracellular molecules. (ahajournals.org)
  • As these molecules are vital for life, metabolic reactions either focus on making these molecules during the construction of cells and tissues, or by breaking them down and using them as a source of energy, by their digestion. (wikipedia.org)
  • The total distance traversed by RGCs extends from the retina, starting from synapses with bipolar and amacrine cells, to the brain, to synapses with neurons in the lateral geniculate nucleus. (nih.gov)
  • PKGI has been detected in the perinuclear region of cells, and recent data indicate that proprotein convertases (PCs) typically resident in the Golgi apparatus (GA) can stimulate PKGI proteolysis and generate a kinase fragment that localizes to the nucleus and regulates gene expression. (sigmaaldrich.com)
  • 9. The unipolar brush cells of the mammalian cerebellum and cochlear nucleus: cytology and microcircuitry. (elsevier.com)
  • Eukaryotes usually have a single nucleus, but a few cell types, such as mammalian red blood cells, have no nuclei, and a few others including osteoclasts have many. (wikipedia.org)
  • The nucleus maintains the integrity of genes and controls the activities of the cell by regulating gene expression-the nucleus is, therefore, the control center of the cell. (wikipedia.org)
  • The nuclear envelope separates the fluid inside the nucleus, called the nucleoplasm, from the rest of the cell. (wikipedia.org)
  • The size of the nucleus depends on the size of the cell it is contained in, with a nucleus typically occupying about 8% of the total cell volume. (wikipedia.org)
  • The nucleus is the largest organelle in animal cells. (wikipedia.org)
  • In mammalian cells, the average diameter of the nucleus is approximately 6 micrometres (µm). (wikipedia.org)
  • He observed a "lumen", the nucleus, in the red blood cells of salmon . (wikipedia.org)
  • Brown was studying orchids under the microscope when he observed an opaque area, which he called the "areola" or "nucleus", in the cells of the flower's outer layer. (wikipedia.org)
  • In 1838, Matthias Schleiden proposed that the nucleus plays a role in generating cells, thus he introduced the name "cytoblast" (cell builder). (wikipedia.org)
  • In 1905 and 1910, the Russian biologist Konstantin Mereschkowski (1855-1921) argued that plastids were reduced cyanobacteria in a symbiosis with a non- photosynthetic ( heterotrophic ) host that was itself formed by symbiosis between an amoeba-like host and a bacterium-like cell that formed the nucleus. (wikipedia.org)
  • The function of the nucleus is to maintain the integrity of these genes and to control the activities of the cell by regulating gene expression . (bionity.com)
  • Brown was studying orchids microscopically when he observed an opaque area, which he called the areola or nucleus, in the cells of the flower's outer layer. (bionity.com)
  • On the left a cell is going through mitosis and its nucleus has disintegrated in preparation of division. (wikidoc.org)
  • A drawing of a cell nucleus published by Walther Flemming in 1882. (wikidoc.org)
  • Describe the structural compartmentation of. (educationindex.com)
  • As in other organisms, the bacterial cell wall provides structural integrity to the cell. (wikipedia.org)
  • In addition, it examines how cell structural networks affect gene and protein signaling networks to produce characteristic phenotypes and cell fate transitions during tissue development. (biologists.org)
  • The Firestop Contractors International Association (FCIA) recommends maintenance of effective compartmentation and structural protection to ensure performance of this critical system during a fire condition. (insulation.org)
  • Thus, raft integrity is a prerequisite for efficient T cell receptor signal transduction. (nih.gov)
  • The bacterium, despite its simplicity, contains a well-developed cell structure which is responsible for some of its unique biological structures and pathogenicity. (wikipedia.org)
  • The resulting SCs will function by enzyme catalysis and/or by gene expression, closely mimicking biological cells with respect to structure and function. (mdpi.com)
  • Current SCs have a cell-like structure and perform some life-like operations, but they still resemble biological cells only superficially. (mdpi.com)
  • These insights teach us that, if we truly want to explain biological regulation and to confront the complexity problem, we must consider how molecular signaling pathways function in the physical context of living cells and tissues. (biologists.org)
  • A demand for non-optical high resolution imaging of live samples promoted rapid development of SICM [2-6] and its successful application to imaging topography of live biological cells where existing microdomains can be imaged. (springer.com)
  • A particular advantage of SICM technique for imaging biological cells is that it requires no physical contact with the sample and operates at nanoscale resolution. (springer.com)
  • The highly successful work of the CRC 593 groups has improved the mechanistic understanding of many biochemical and cell biological pathways. (uni-marburg.de)
  • The biological research was conducted with a wide spectrum of model organisms such as yeast, filamentous fungi, various pathogens, cell culture systems and transgenic animals using state-of-the-art instrumentation including three core facilities in Proteomics, Protein Spectroscopy, and Bioimaging. (uni-marburg.de)
  • This mechanism might be responsible for mast cell accumulation in allergic tissues. (aspetjournals.org)
  • Explain the basic mechanisms of homeostasis by integrating the functions of cells, tissues, organs, and organ systems. (brandman.edu)
  • abstract = "Following exposure of L1210 leukemia cells to antifolates, tetrahydrofolate-dependent purine and pyrimidine biosyntheses are blocked despite the presence of the major portion of tetrahydrofolate cofactors. (elsevier.com)
  • The small heat shock protein Hsp25 is constitutively expressed in the adult mouse cerebellum by parasagittal stripes of Purkinje cells confined to the caudal central zone ( approximately lobules VI and VII), the nodular zone ( approximately ventral lobule IX and lobule X), and the paraflocculi/flocculi. (semanticscholar.org)
  • 22. Characterization of purkinje cells in the goldfish cerebellum during eye movement and adaptive modification of the vestibulo-ocular reflex. (elsevier.com)
  • 2. Zebrins: Molecular Markers of Compartmentation in the Cerebellum. (indigo.ca)
  • Despite the homogenous cytoarchitecture of the cerebellum (Cb), there exists an intricate array of parasagittal stripes of cells defined by their unique molecular and physiological characteristics. (jneurosci.org)
  • Despite detailed knowledge of specific enzymes and pathways that utilize these coenzymes, a holistic understanding of the regulation and compartmentalization of NADH- and NADPH-dependent pathways is lacking, partly because of a lack of tools with which to investigate these processes in living cells. (nature.com)
  • Regulation at all levels is considered, from initial perception of the growth signal, through transduction responses and DNA replication, to the 'ultimate' level of cell expansion. (indigo.ca)
  • It was shown later that implementation of immune response in germinal centers involves (besides T-helpers, follicular T-helpers) T-regulatory cells that could produce a positive effect in FL owing to specific features of the regulation or B-cell response. (scirp.org)
  • Enzymes act as catalysts - they allow a reaction to proceed more rapidly - and they also allow the regulation of the rate of a metabolic reaction, for example in response to changes in the cell's environment or to signals from other cells. (wikipedia.org)
  • ROS overproduction is a feature of cancer cells and plays several roles during the natural history of malignant tumor. (hindawi.com)
  • A transformed cell is identified by the loss of control of proliferation and deregulation of apoptosis producing an excess of cell number and forming a mass (tumor). (hindawi.com)
  • Natural killer (NK) cells are cytotoxic lymphocytes of the innate immune system that are essential for the control of infections and for tumor immunosurveillance ( 1 , 2 ). (sciencemag.org)
  • Falaleeva N. A., Osmanov E. A., Tupitsyn N. N. Federal State Budgetary Institute N. N. Blokhin Russian Cancer Research Center, Health Ministry of Russian Federation, Moscow, Russia SUMMARY Follicular dendritic cells, expressing Fc ε RII or CD23 (Fc ε RIIFDCs) as a component of non-tumor environment have been studied in 232 follicular lymphoma (FL) patients. (scirp.org)
  • Mandatory criteria include confirmed mature B-cell nature of the tumor, CD20 expression, and if needing additional markers (bcl-2, bcl-6, MUM- 1), B-cell clonality and other criteria are used. (scirp.org)
  • As a whole the tumor content is polymorphous with T-cells, macrophages, follicular dendritic cells (FDCs) found within malignant germinal centers besides B-cells. (scirp.org)
  • At the beginning of the 21st century the investigators paid attention to the fact that this hemopoietic tissue tumor was in many respects regulated by cells of so called non-tumor environment, i.e. (scirp.org)
  • Infiltration of tumor tissue by immune cells was reflected by gene expression profiles in the tumor tissue. (scirp.org)
  • Two types of immune response were defined accordingly that were associated with microenvironment cells infiltrating the tumor. (scirp.org)
  • Antigen compartmentation and T helper cell tolerance induction. (rupress.org)
  • Coreceptors CD28 and cytotoxic T lymphocyte antigen (CTLA)-4 have opposing effects on TcR/CD3 activation of T cells. (rupress.org)
  • T cell activation is mediated by the antigen-receptor complex (TcRζ/CD3) and coreceptors CD28, inducible costimulator (ICOS), and cytotoxic T lymphocyte antigen (CTLA)-4 (CD152) ( 1 , 2 ). (rupress.org)
  • Various imaging studies have established that T cells that engage antigen-presenting cells (APCs) bearing stimulatory MHC-peptide complexes undergo macromolecular rearrangements that result in the formation of an immune synapse. (rupress.org)
  • I. Development and compartmentation of cerebellar cortex. (elsevier.com)
  • Unipolar brush cells in cerebellar cortex. (elsevier.com)
  • 1. Purkinje Cell Heterogeneity: Its Role in Organizing the Topography of the Cerebellar Cortex Connections. (indigo.ca)
  • Retinal ganglion cells (RGCs) are specialized projection neurons that relay an immense amount of visual information from the retina to the brain. (nih.gov)
  • The RGC cell body is larger than other retinal neurons, but is still only a very small fraction (one ten thousandths) of the length and total surface area of the axon. (nih.gov)
  • Histamine is synthesized from l -histidine by histidine decarboxylation in specific cell types, such as mast cells, basophils, enterochromaffin-like cells, and neurons. (aspetjournals.org)
  • The embryonic ventricular zone (VZ) of the cerebral cortex contains migrating neurons, radial glial cells, and a large population of cycling progenitor cells that generate newborn neurons. (jneurosci.org)
  • Because the vast majority cycling cells in the cortical VZ have characteristics of radial glia, the radial glial precursor cell may be responsible for both the production of newborn neurons and the guidance of daughter neurons to their destinations in the developing cortex. (jneurosci.org)
  • The ventricular zone (VZ) of the embryonic cerebral cortex is a pseudostratified neuroepithelium that contains the precursor cells for most excitatory neurons contributing to the adult neocortex. (jneurosci.org)
  • The number of functional cell-surface NMDARs in cortical neurons increases 60-100% within 10 minutes of exposure to PregS, as shown by surface biotinylation and affinity purification. (aspetjournals.org)
  • Rap1 is a small GTPase that controls diverse processes, such as cell adhesion, cell-cell junction formation and cell polarity. (genome.jp)
  • Potter CM, Schobesberger S, Lundberg MH, Weinberg PD, Mitchell JA, Gorelik J. Shape and compliance of endothelial cells after shear stress in vitro or from different aortic regions: scanning ion conductance microscopy study. (springer.com)
  • Side-specific mechanical properties of valve endothelial cells. (springer.com)
  • Endothelial cells (EC) metabolize l -arginine mainly by arginase, which exists as two distinct isoforms, arginase I and II. (physiology.org)
  • Caveolin-1 and -2 are coexpressed and abundantly expressed in adipocytes, endothelial cells, and fibroblasts. (ahajournals.org)
  • All mammalian cells are eukaryotic, and whilst the eukaryotic type of cell is not exclusive to mammals, mammalian cells differ from other eukaryotic cells because of the organelles that are or are not present. (educationindex.com)
  • Glycosomes are heterogeneous organelles that likely exist as sub-populations with different protein composition and function in a given cell, between individual cells, and between species. (portlandpress.com)
  • Caveolae are omega-shaped organelles of the cell surface. (ahajournals.org)
  • Expression of heat-shock protein Hsp25 in mouse Purkinje cells during development reveals novel features of cerebellar compartmentation. (semanticscholar.org)
  • The Golgi apparatus regulates cGMP-dependent protein kinase I compartmentation and proteolysis. (sigmaaldrich.com)
  • cGMP-dependent protein kinase I (PKGI) is an important effector of cGMP signaling that regulates vascular smooth muscle cell (SMC) phenotype and proliferation. (sigmaaldrich.com)
  • Differential targeting and retention of G protein-coupled receptors in polarized epithelial cells. (springer.com)
  • Furthermore, each En gene has unique as well as overlapping functions in patterning the ML molecular code and each En protein has dominant functions in different AP domains (subsets of lobules). (jneurosci.org)
  • Functional cloning of candidate genes that regulate purkinje cell-specific gene expression. (elsevier.com)
  • The genes within these chromosomes are structured in such a way to promote cell function. (wikipedia.org)
  • Given that En2 might regulate ML molecular code patterning, and because the En transcription factors are known to have extensive functional overlap in regulating foliation, we analyzed an allelic series of viable En1/2 mutants to test whether the two genes together play a broad role in regulating ML molecular coding throughout the vermis. (jneurosci.org)
  • If one bathes cells in a catecholamine, such as epinephrine, β -adrenergic receptors are activated and a cAMP signal is generated in the cytosol. (aspetjournals.org)
  • An education process ensures that activating receptors promote NK cell activation in response to infected or abnormal cells and that inhibitory receptors prevent inappropriate NK cell activation by healthy cells. (sciencemag.org)
  • Cells that lack inhibitory receptors are hyporesponsive. (sciencemag.org)
  • Interfering with receptor dynamics reduced signaling by NKp46, indicating that the dynamic movements of these receptors are likely important determinants of NK cell responsiveness. (sciencemag.org)
  • In addition, Northern blot analysis showed the expression of H 4 but not H 3 receptors on mast cells. (aspetjournals.org)
  • Therefore, we concluded that the elaboration of the major leaf alkaloids involves the participation of at least two cell types and requires the intercellular translocation of a pathway intermediate. (plantcell.org)
  • Thousands of potential combinations of these three elements are possible in any given cell type, making the characterization of cAMP signaling compartments daunting. (aspetjournals.org)
  • Organic anions were transported across the cytoplasm of the principal cells both by diffusion and in vesicles. (biologists.org)
  • 26. What features of visually guided arm movements are encoded in the simple spike discharge of cerebellar Purkinje cells. (elsevier.com)
  • 9. Voltage- and Transmitter-Gated Channels in Purkinje Cells from Organotypic Cerebellar Cultures. (indigo.ca)
  • Higher termites are characterized by a purely prokaryotic gut microbiota and an increased compartmentation of their intestinal tract. (asm.org)
  • Eukaryotic cells are typically much larger than those of prokaryotes having a volume of around 10,000 times greater than the prokaryotic cell. (wikipedia.org)
  • While CD28 increased expression and the number of peripheral T cells induced to express surface rafts in response to TcR ligation, CTLA-4 potently inhibited both TcR and TcR × CD28 induced raft expression on the surface of T cells. (rupress.org)
  • Our observations demonstrate for the first time that CTLA-4 targets the release of rafts to the surface of T cells, and provides a mechanism for the opposing effects of CD28 and CTLA-4 on costimulation. (rupress.org)
  • While the integrity of rafts is required for efficient T cell activation ( 22 ), the engagement of the TcR can promote its movement into rafts ( 23 ). (rupress.org)
  • In this context, memory and effector T cells have been reported to express higher levels of rafts than resting T cells ( 25 ). (rupress.org)
  • Although CD28 promotes raft expression, there has been a lack of evidence on whether alterations in the formation of surface rafts can explain the opposing effects of CD28 and CTLA-4 on the costimulation of T cells. (rupress.org)
  • In this study, we report that while CD28 greatly increased the number of GM1 negative peripheral T cells that become positive as a result of TcR ligation, CTLA-4 profoundly inhibited the expression of surface rafts. (rupress.org)
  • This was confirmed biochemically where CD28 augmented the detection of the adaptor LAT in purified rafts, while CTLA-4 coligation blocked this increase at the level of resting T cells. (rupress.org)
  • Based on the kinetic properties of the phosphate transport system of tobacco BY-2 cells, it was demonstrated that phosphite inhibited phosphate uptake in a competitive manner. (biomedsearch.com)
  • To directly follow the fate of phosphate and phosphite in cytoplasmic and vacuolar pools of tobacco cells, we took advantage of the pH-sensitive chemical shift of the Phi anion. (biomedsearch.com)
  • The course emphasizes macromolecular organization and compartmentation of cellular activities. (reed.edu)
  • Molecular Markers for Ion Compartmentation in Cells of Higher Plants 1. (sussex.ac.uk)
  • In this study, a combination of physiological characteristics and iTRAQ-based proteomic approaches was conducted to investigate the molecular mechanisms underlying the salt response of suspension cell cultures of halophytic Halogeton glomeratus . (frontiersin.org)
  • The remarkable deformability of the human red blood cell (RBC) results from the coupled dynamic response of the phospholipid bilayer and the spectrin molecular network. (pnas.org)
  • Microalgae, including diatoms, apply physiological and molecular strategies such as phosphorus scavenging or recycling as well as adjusting cell growth in order to adapt to limiting phosphorus concentrations. (royalsocietypublishing.org)
  • Gene expression analysis of an allelic series of En1/2 mutant mice that have an intact Purkinje cell layer revealed severe patterning defects using three known components of the ML molecular code and a new marker of Hsp25 negative stripes (Neurofilament heavy chain, Nfh). (jneurosci.org)
  • First, several CRC groups were interested in the elucidation of the molecular mechanisms of cellular compartmentalization processes in eukaryotic cells. (uni-marburg.de)
  • Previous studies from this laboratory demonstrated that this cannot be due to direct inhibition of thymidylate synthase by dihydrofolate polyglutamates or other endogenous folates and suggested that this phenomenon is due to compartmentation of tetrahydrofolate cofactors unavailable for interconversion and/or oxidation when dihydrofolate reductase activity is abolished by antifolates. (elsevier.com)
  • [1] Unlike mammalian red blood cells, those of other vertebrates still contain nuclei. (wikipedia.org)
  • Here, we will refer to all these cell-like systems shortly as synthetic cells (SCs), but most of the discussion will be focused on SCs built from biomolecules as DNA, RNA, ribosomes, enzymes, etc. encapsulated within liposomes. (mdpi.com)
  • Here, we provide an overview of the role of spatially arranged enzymatic networks, catalyzed by creatine kinase, adenylate kinase, carbonic anhydrase and glycolytic enzymes, in efficient high-energy phosphoryl transfer and signal communication in the cell. (biologists.org)
  • Organisation of enzymes in the cell. (amrita.edu)
  • The enzyme lysozyme, found in human tears, also digests the cell wall of bacteria and is the body's main defense against eye infections. (wikipedia.org)
  • Oxygen-hemoglobin dissociation curves: effect of inherited enzyme defects of the red cell, Science, 165, 601-602. (ximicat.com)
  • CTLA-4 engagement inhibits IL-2 accumulation and cell cycle progression upon activation of resting T cells. (rupress.org)
  • However, the role of the endomembrane system in PKGI compartmentation and processing is unknown. (sigmaaldrich.com)
  • These studies detail a role for the endomembrane system in regulating PKGI compartmentation and proteolysis. (sigmaaldrich.com)
  • Trafficking of endogenous smooth muscle cell cholesterol: a role for serum amyloid A and interleukin-1β. (nih.gov)
  • The relevance of the lipid composition of the tonoplast to its central role in ion compartmentation within the halophyte cell is discussed. (sussex.ac.uk)
  • Enforcement and release of quiescence in cells in the embryo axis of the seed: role of the nuclear proteins13. (indigo.ca)
  • The role of compartmentation in the control of glycolysis, Curr. (ximicat.com)
  • The initial anatomical descriptions of the cells of the VZ were published over 100 years ago by several pioneering neuroanatomists, including Kölliker, His, Golgi, Magini, and Ramon y Cajal (for review, see Bentivoglio and Mazzarello, 1999 ). (jneurosci.org)
  • Both Kölliker (1896) and His (1889) showed the existence of a neuroepithelium lining the ventricular system, and Kölliker (1896) also demonstrated, using Golgi impregnations, that cells of this layer possessed long radial fibers that extended into the cortical mantle. (jneurosci.org)
  • The present study shows that histamine induces chemotaxis of mouse mast cells, without affecting mast cell degranulation. (aspetjournals.org)
  • T cell interleukin-17 induces stromal cells to produce proinflammatory and hematopoietic cytokines. (rupress.org)
  • This leads to a method for predicting cell maintenance costs on the basis of the measured flux ratio between the oxidative steps of the oxidative pentose phosphate pathway and glycolysis. (wiley.com)
  • Cytotoxicity of high doses of vitamin C in cancer cells appears to be related to excessive reactive oxygen species generation and the resulting suppression of the energy production via glycolysis. (mdpi.com)
  • A hallmark of cancer cells is a strongly upregulated aerobic glycolysis, which elevates its relative importance as a source of ATP (Adenosine 5′-triphosphate). (mdpi.com)
  • The central and rightmost cell are in interphase , thus their entire nuclei are labeled. (wikipedia.org)
  • Franz Meyen was a strong opponent of this view, having already described cells multiplying by division and believing that many cells would have no nuclei. (wikipedia.org)
  • Konstantin Mereschkowski proposed a symbiotic origin for cells with nuclei. (wikipedia.org)
  • In his 1938 work Titres et Travaux Scientifiques , Chatton had proposed the two terms, calling the bacteria prokaryotes and organisms with nuclei in their cells eukaryotes. (wikipedia.org)
  • 5. The physiological effects of serotonin on spontaneous and amino acid-induced activation of cerebellar nuclear cells: an in vivo study in the cat. (elsevier.com)
  • ROS concentration and compartmentation determine their physiological or pathological effects. (hindawi.com)
  • High-Resolution Analysis of Gut Environment and Bacterial Microbiota Reveals Functional Compartmentation of the Gut in Wood-Feeding Higher Termites (Nasutitermes spp. (asm.org)
  • This discovery raises the possibility that radial glia and the population of VZ progenitor cells may be one anatomical and functional cell class. (jneurosci.org)
  • For instance some plant cells have chloroplasts which are not present in mammalian cells, but both plant cells and mammalian cells are eukaryotic in nature. (educationindex.com)
  • However commencing 2 weeks after treatment there was a marked decrease in the number of Cldn1-positive basal cells, and the zone of Cldn1-null epidermal cells was expanded up into the lower stratified epidermis throughout the progression of DMBA/TPA treatment. (biomedcentral.com)
  • We show that accounting for transport and maintenance costs substantially improves the accuracy of fluxes predicted from a flux balance model of heterotrophic Arabidopsis cells in culture, irrespective of the objective function used in the analysis. (wiley.com)
  • Fluorescence microscopy showed that transport of the carboxylate fluorescein was confined to the principal cells of the main (secretory) segment and all the cells of the lower (reabsorptive) segment. (biologists.org)
  • Nuclear transport is of paramount importance to cell function, as movement through the pores is required for both gene expression and chromosomal maintenance. (bionity.com)
  • Nuclear transport is crucial to cell function, as movement through the pores is required for both gene expression and chromosomal maintenance. (wikidoc.org)
  • Cell Processes: Photosynthesis Part 1: Pigments in Plant Leaves Purpose: The purpose of this experiment is to visualize the pigments present in plant leaves by using paper chromatography. (educationindex.com)
  • Ramon y Cajal's (1911) illustrations of embryonic cortex clearly show radial processes extending from the cells of the periventricular neuroepithelium and terminating in branches at the pial surface of the cortex. (jneurosci.org)
  • This structure develops over 5-30 min as a result of active cytoskeletal processes and subdivides the T cell-APC interface into two concentric zones: the central and peripheral supramolecular activation clusters (cSMAC and pSMAC) (for review see Delon and Germain, 2000 ). (rupress.org)
  • Moreover, when the new method was applied to cells under control, elevated temperature and hyper-osmotic conditions, only elevated temperature led to a substantial increase in cell maintenance costs. (wiley.com)
  • It is concluded that the hyper-osmotic conditions tested did not impose a metabolic stress, in as much as the metabolic network is not forced to devote more resources to cell maintenance. (wiley.com)
  • Nucleosomal peptide epitopes for nephritis-inducing T helper cells of murine lupus. (rupress.org)
  • There were marked sex differences in blood corticosterone compartmentation in rats, which were unrelated to testosterone. (jove.com)
  • An in-depth study of the structure-function relationships within eukaryotic cells. (reed.edu)
  • The latter two cell classes have been assumed for some time to be distinct in both function and anatomy, but the cellular anatomy of the progenitor cell type has remained poorly defined. (jneurosci.org)
  • High-resolution scanning patch-clamp: new insights into cell function. (springer.com)
  • This is important because spermatozoa and their cell-surface antigens appear long after 'self' tolerance is established, and a compromise in BTB function would result in the host producing antibodies against its own sperm. (royalsocietypublishing.org)