The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Pairing of purine and pyrimidine bases by HYDROGEN BONDING in double-stranded DNA or RNA.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
That segment of commercial enterprise devoted to the design, development, and manufacture of chemical products for use in the diagnosis and treatment of disease, disability, or other dysfunction, or to improve function.
The relative amounts of the PURINES and PYRIMIDINES in a nucleic acid.
Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The reformation of all, or part of, the native conformation of a nucleic acid molecule after the molecule has undergone denaturation.
Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.
A group of 13 or more deoxyribonucleotides in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.
A highly fluorescent anti-infective dye used clinically as a topical antiseptic and experimentally as a mutagen, due to its interaction with DNA. It is also used as an intracellular pH indicator.
The aggregate business enterprise of agriculture, manufacture, and distribution related to tobacco and tobacco-derived products.
Activity involved in transfer of goods from producer to consumer or in the exchange of services.
Any enterprise centered on the processing, assembly, production, or marketing of a line of products, services, commodities, or merchandise, in a particular field often named after its principal product. Examples include the automobile, fishing, music, publishing, insurance, and textile industries.
Deoxyribonucleic acid that makes up the genetic material of viruses.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Constituent of the 40S subunit of eukaryotic ribosomes. 18S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Polydeoxyribonucleotides made up of deoxyadenine nucleotides and thymine nucleotides. Present in DNA preparations isolated from crab species. Synthetic preparations have been used extensively in the study of DNA.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
The inferior region of the skull consisting of an internal (cerebral), and an external (basilar) surface.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
Any of the covalently closed DNA molecules found in bacteria, many viruses, mitochondria, plastids, and plasmids. Small, polydisperse circular DNA's have also been observed in a number of eukaryotic organisms and are suggested to have homology with chromosomal DNA and the capacity to be inserted into, and excised from, chromosomal DNA. It is a fragment of DNA formed by a process of looping out and deletion, containing a constant region of the mu heavy chain and the 3'-part of the mu switch region. Circular DNA is a normal product of rearrangement among gene segments encoding the variable regions of immunoglobulin light and heavy chains, as well as the T-cell receptor. (Riger et al., Glossary of Genetics, 5th ed & Segen, Dictionary of Modern Medicine, 1992)
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
A genus of ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES.
Condensation products of aromatic amines and aldehydes forming azomethines substituted on the N atom, containing the general formula R-N:CHR. (From Grant & Hackh's Chemical Dictionary, 5th ed)
The act or practice of calling public attention to a product, service, need, etc., especially by paid announcements in newspapers, magazines, on radio, or on television. (Random House Unabridged Dictionary, 2d ed)
Agents that are capable of inserting themselves between the successive bases in DNA, thus kinking, uncoiling or otherwise deforming it and therefore preventing its proper functioning. They are used in the study of DNA.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
The interchange of goods or commodities, especially on a large scale, between different countries or between populations within the same country. It includes trade (the buying, selling, or exchanging of commodities, whether wholesale or retail) and business (the purchase and sale of goods to make a profit). (From Random House Unabridged Dictionary, 2d ed, p411, p2005 & p283)
A situation in which an individual might benefit personally from official or professional actions. It includes a conflict between a person's private interests and official responsibilities in a position of trust. The term is not restricted to government officials. The concept refers both to actual conflict of interest and the appearance or perception of conflict.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
A purine base and a fundamental unit of ADENINE NUCLEOTIDES.
An order of fungi in the phylum Ascomycota that multiply by budding. They include the telomorphic ascomycetous yeasts which are found in a very wide range of habitats.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
Ribonucleic acid that makes up the genetic material of viruses.
The promotion and maintenance of physical and mental health in the work environment.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.
A purine or pyrimidine base bonded to a DEOXYRIBOSE containing a bond to a phosphate group.
Health services for employees, usually provided by the employer at the place of work.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)
The moral obligations governing the conduct of commercial or industrial enterprises.
A group of adenine ribonucleotides in which the phosphate residues of each adenine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.
Viruses whose host is Escherichia coli.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The rate dynamics in chemical or physical systems.
A group of ribonucleotides (up to 12) in which the phosphate residues of each ribonucleotide act as bridges in forming diester linkages between the ribose moieties.
The genetic process of crossbreeding between genetically dissimilar parents to produce a hybrid.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
The functional hereditary units of BACTERIA.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
The monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose sugar, and a phosphate group. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
Relations of an individual, association, organization, hospital, or corporation with the publics which it must take into consideration in carrying out its functions. Publics may include consumers, patients, pressure groups, departments, etc.
The industry concerned with processing, preparing, preserving, distributing, and serving of foods and beverages.
Ribonucleic acid in fungi having regulatory and catalytic roles as well as involvement in protein synthesis.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Insurance providing for payment of a stipulated sum to a designated beneficiary upon death of the insured.
A process whereby representatives of a particular interest group attempt to influence governmental decision makers to accept the policy desires of the lobbying organization.
Use for articles on the investing of funds for income or profit.
The functional hereditary units of VIRUSES.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
Diseases caused by factors involved in one's employment.
Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
Place or physical location of work or employment.
Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES.
Application of marketing principles and techniques to maximize the use of health care resources.
The sum of the weight of all the atoms in a molecule.
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
Enzymes that catalyze the hydrolysis of ester bonds within RNA. EC 3.1.-.
Enzymes that catalyze the hydrolysis of the internal bonds and thereby the formation of polynucleotides or oligonucleotides from ribo- or deoxyribonucleotide chains. EC 3.1.-.
The relationships of groups of organisms as reflected by their genetic makeup.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Established cell cultures that have the potential to propagate indefinitely.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
The bestowing of tangible or intangible benefits, voluntarily and usually without expectation of anything in return. However, gift giving may be motivated by feelings of ALTRUISM or gratitude, by a sense of obligation, or by the hope of receiving something in return.
The process by which a DNA molecule is duplicated.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Neoplasms of the base of the skull specifically, differentiated from neoplasms of unspecified sites or bones of the skull (SKULL NEOPLASMS).
Travel to another country for the purpose of medical treatment.
The exposure to potentially harmful chemical, physical, or biological agents that occurs as a result of one's occupation.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Exclusive legal rights or privileges applied to inventions, plants, etc.
Intentional falsification of scientific data by presentation of fraudulent or incomplete or uncorroborated findings as scientific fact.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
The process of cumulative change over successive generations through which organisms acquire their distinguishing morphological and physiological characteristics.
The presence of an uncomplimentary base in double-stranded DNA caused by spontaneous deamination of cytosine or adenine, mismatching during homologous recombination, or errors in DNA replication. Multiple, sequential base pair mismatches lead to formation of heteroduplex DNA; (NUCLEIC ACID HETERODUPLEXES).
Organizations which assume the financial responsibility for the risks of policyholders.
Exercise of governmental authority to control conduct.
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
The provision of monetary resources including money or capital and credit; obtaining or furnishing money or capital for a purchase or enterprise and the funds so obtained. (From Random House Unabridged Dictionary, 2d ed.)
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Administration and functional structures for the purpose of collectively systematizing activities for a particular goal.
The effort of two or more parties to secure the business of a third party by offering, usually under fair or equitable rules of business practice, the most favorable terms.
Computer-based representation of physical systems and phenomena such as chemical processes.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
That portion of total HEALTH CARE COSTS borne by an individual's or group's employing organization.
The part of a denture that overlies the soft tissue and supports the supplied teeth and is supported in turn by abutment teeth or the residual alveolar ridge. It is usually made of resins or metal or their combination.
Proteins found in any species of bacterium.
Unforeseen occurrences, especially injuries in the course of work-related activities.
Financial support of research activities.
That distinct portion of the institutional, industrial, or economic structure of a country that is controlled or owned by non-governmental, private interests.
The principles of proper professional conduct concerning the rights and duties of the pharmacist, relations with patients and fellow practitioners, as well as actions of the pharmacist in health care and interpersonal relations with patient families. (From Stedman, 25th ed)
Proteins found in any species of virus.
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
The obligations and accountability assumed in carrying out actions or ideas on behalf of others.
The aggregate enterprise of manufacturing and technically producing chemicals. (From Random House Unabridged Dictionary, 2d ed)
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
The act of deceiving or the fact of being deceived.
Process that is gone through in order for a drug to receive approval by a government regulatory agency. This includes any required pre-clinical or clinical testing, review, submission, and evaluation of the applications and test results, and post-marketing surveillance of the drug.
An agency of the PUBLIC HEALTH SERVICE concerned with the overall planning, promoting, and administering of programs pertaining to maintaining standards of quality of foods, drugs, therapeutic devices, etc.
The deliberate attempt to influence attitudes and beliefs for furthering one's cause or damaging an opponent's cause.
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
Exploitation through misrepresentation of the facts or concealment of the purposes of the exploiter.
Institutional funding for facilities and for equipment which becomes a part of the assets of the institution.
The industry concerned with the removal of raw materials from the Earth's crust and with their conversion into refined products.
Units that convert some other form of energy into electrical energy.
Form in which product is processed or wrapped and labeled. PRODUCT LABELING is also available.
Economic sector concerned with the provision, distribution, and consumption of health care services and related products.
Substances and materials manufactured for use in various technologies and industries and for domestic use.
Inhaling and exhaling the smoke of burning TOBACCO.
A course or method of action selected, usually by a government, from among alternatives to guide and determine present and future decisions.
Chronic absence from work or other duty.
A tough, malleable, iron-based alloy containing up to, but no more than, two percent carbon and often other metals. It is used in medicine and dentistry in implants and instrumentation.
The organization, management, and assumption of risks of a business or enterprise, usually implying an element of change or challenge and a new opportunity.
Naturally occurring complex liquid hydrocarbons which, after distillation, yield combustible fuels, petrochemicals, and lubricants.
The complex of political institutions, laws, and customs through which the function of governing is carried out in a specific political unit.
Formal relationships established between otherwise independent organizations. These include affiliation agreements, interlocking boards, common controls, hospital medical school affiliations, etc.
Drugs whose drug name is not protected by a trademark. They may be manufactured by several companies.
Planning, organizing, and administering all activities related to personnel.
Revealing of information, by oral or written communication.
Management control systems for structuring health care delivery strategies around case types, as in DRGs, or specific clinical services.
The interactions between representatives of institutions, agencies, or organizations.
The science, art, or technology dealing with processes involved in the separation of metals from their ores, the technique of making or compounding the alloys, the techniques of working or heat-treating metals, and the mining of metals. It includes industrial metallurgy as well as metallurgical techniques employed in the preparation and working of metals used in dentistry, with special reference to orthodontic and prosthodontic appliances. (From Jablonski, Dictionary of Dentistry, 1992, p494)
An organizational enterprise between a public sector agency, federal, state or local, and a private sector entity. Skills and assets of each sector are shared to deliver a service or facility for the benefit or use of the general public.
AUTOMOBILES, trucks, buses, or similar engine-driven conveyances. (From Random House Unabridged Dictionary, 2d ed)
An absence from work permitted because of illness or the number of days per year for which an employer agrees to pay employees who are sick. (Webster's New Collegiate Dictionary, 1981)
Organizations which are not operated for a profit and may be supported by endowments or private contributions.
The state of being engaged in an activity or service for wages or salary.
Time period from 1901 through 2000 of the common era.
The process of finding chemicals for potential therapeutic use.
Predetermined sets of questions used to collect data - clinical data, social status, occupational group, etc. The term is often applied to a self-completed survey instrument.
Organizations established by endowments with provision for future maintenance.
The interaction of persons or groups of persons representing various nations in the pursuit of a common goal or interest.
Spread and adoption of inventions and techniques from one geographic area to another, from one discipline to another, or from one sector of the economy to another. For example, improvements in medical equipment may be transferred from industrial countries to developing countries, advances arising from aerospace engineering may be applied to equipment for persons with disabilities, and innovations in science arising from government research are made available to private enterprise.
Transmission of information over distances via electronic means.
Systematic gathering of data for a particular purpose from various sources, including questionnaires, interviews, observation, existing records, and electronic devices. The process is usually preliminary to statistical analysis of the data.
Physiological or psychological effects of periods of work which may be fixed or flexible such as flexitime, work shifts, and rotating shifts.
The promotion and support of consumers' rights and interests.
Crafts, trades, professions, or other means of earning a living.
Health care institutions operated by private groups or corporations for a profit.
Laws concerned with manufacturing, dispensing, and marketing of drugs.
Process of shifting publicly controlled services and/or facilities to the private sector.
Works about pre-planned studies of the safety, efficacy, or optimum dosage schedule (if appropriate) of one or more diagnostic, therapeutic, or prophylactic drugs, devices, or techniques selected according to predetermined criteria of eligibility and observed for predefined evidence of favorable and unfavorable effects. This concept includes clinical trials conducted both in the U.S. and in other countries.
Oils which are used in industrial or commercial applications.
The obtaining and management of funds for institutional needs and responsibility for fiscal affairs.
Systematic organization, storage, retrieval, and dissemination of specialized information, especially of a scientific or technical nature (From ALA Glossary of Library and Information Science, 1983). It often involves authenticating or validating information.
Time period from 2001 through 2100 of the common era.
Organizational activities previously performed internally that are provided by external agents.
Air pollutants found in the work area. They are usually produced by the specific nature of the occupation.
An office in the Department of Labor responsible for developing and establishing occupational safety and health standards.
Instruments or technological means of communication that reach large numbers of people with a common message: press, radio, television, etc.
The reciprocal interaction of two or more professional individuals.
Drugs intended for human or veterinary use, presented in their finished dosage form. Included here are materials used in the preparation and/or formulation of the finished dosage form.
The total amount of work to be performed by an individual, a department, or other group of workers in a period of time.
Medical specialty concerned with the promotion and maintenance of the physical and mental health of employees in occupational settings.
The amount that a health care institution or organization pays for its drugs. It is one component of the final price that is charged to the consumer (FEES, PHARMACEUTICAL or PRESCRIPTION FEES).
Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.
Health insurance plans for employees, and generally including their dependents, usually on a cost-sharing basis with the employer paying a percentage of the premium.
The productive enterprises concerned with food processing.
Coverage by contract whereby one part indemnifies or guarantees another against loss by a specified contingency.
Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)
Governmental levies on property, inheritance, gifts, etc.
Collections of facts, assumptions, beliefs, and heuristics that are used in combination with databases to achieve desired results, such as a diagnosis, an interpretation, or a solution to a problem (From McGraw Hill Dictionary of Scientific and Technical Terms, 6th ed).
Elements, compounds, mixtures, or solutions that are considered severely harmful to human health and the environment. They include substances that are toxic, corrosive, flammable, or explosive.
Blom N, Gammeltoft S, Brunak S (1999). "Sequence and structure-based prediction of eukaryotic protein phosphorylation sites". ... PB-CD4+ T cells, PB-CD8+ T cells, lymph node, lung, thymus, thyroid, leukemia chronic myelogenous K562, and leukemia ... "SUMOplot Analysis Program". Abgent- a WuXi AppTec Company. Retrieved 14 May 2013. Johansen MB, Kiemer L, Brunak S (2006). " ... There is also an alternate form of CCDC130 where only 803 bases are transcribed instead of 1433 bases, but there is no ...
Scientists first converted poliovirus's published RNA sequence, 7741 bases long, into a DNA sequence, as DNA was easier to ... The mechanism of viral release from the cell is unclear, but each dying cell can release up to 10,000 polio virions. Drake ... The complete viral genome was then assembled by a gene synthesis company. Nineteen markers were incorporated into the ... Mueller S, Wimmer E (August 2003). "Recruitment of nectin-3 to cell-cell junctions through trans-heterophilic interaction with ...
7741 bases long, into a DNA sequence, as DNA was easier to synthesize. Short fragments of this DNA sequence were obtained by ... Follicular dendritic cell sarcoma. Extranodal NK/T-cell lymphoma, nasal type. MCPyV Merkel-cell carcinoma. RNA virus. HCV ... The complete viral genome was then assembled by a gene synthesis company. This whole painstaking process took two years. ... The mechanism of viral release from the cell is unclear,[2] but each dying cell can release up to 10,000 polio virions.[26] ...
Based on 16S ribosomal RNA sequences, Bala et al. renamed the species in 2004 Amycolatopsis rifamycinica. Rifamycins were first ... This is due to the observation that 3 amino-acid analogues converted into AHBA in cell-free extracts of A. mediterranei. The ... The name was originally given by two microbiologists working with the Italian drug company Group Lepetit SpA in Milan, the ... In 1969, the bacterium was renamed Nocardia mediterranei when another scientist named Thiemann found that it has a cell wall ...
For libraries below ~20,000 bases, read in circular consensus sequencing, yield per cell is quoted at 450 GB or about 30 GB of ... based on the properties of zero-mode waveguides. The company was founded based on research done at Cornell University that ... In 2015, the company launched a new sequencing instrument called the Sequel System with approximately 7-fold greater capacity ... The cells for the RS sequencer are sold in packs of eight. Cells for the Sequel or Sequel II sequencers are sold in packs of ...
... a Bengaluru-based information technology company involved in microarray data analytics and sequencing. and has delivered ... keynote addresses which included the discourse on Engineering Reversal of Drug Resistance in Cancer Cells at the Drug Discovery ...
In 2007 it was reported that several companies were offering synthesis of genetic sequences up to 2000 base pairs (bp) long, ... Cell-based therapies focus on immunotherapies, mostly by engineering T cells. T cell receptors were engineered and 'trained' to ... DNA sequencing determines the order of nucleotide bases in a DNA molecule. Synthetic biologists use DNA sequencing in their ... "Constructing vesicle-based artificial cells with embedded living cells as organelle-like modules". Scientific Reports. 8 (1): ...
The company uses a technological platform for its research, which is based on adenoviruses to introduce human gene sequences ... into a wide variety of human cell lines to knock-in or knock-down specific proteins. In 2019 Gilead Sciences and Galapagos ... The company later changed its name to Galapagos NV and completed its initial public offering on Euronext Amsterdam and Euronext ... The company develops drugs against rheumatoid arthritis, crohn's disease, ulcerative colitis, psoriasis, systemic lupus ...
His work has impact in various fields of mass spectrometry-based proteomics: The peptide sequence tag approach developed at the ... From his research group in Munich originated in 2016 PreOmics - a company commercializing sample prep sets, and EVOSEP - a ... A recently developed metabolic labeling technique called SILAC (stable isotope labeling with amino acids in cell culture) is ... Nano-electrospray (an electrospray technique with very low flow rates) was the first method that allowed femtomole sequencing ...
... polymerase-based sequence-by-synthesis on the Illumina (company) platform, or sequencing by ligation on the ABI Solid ... Examples of such findings are in embryonic stem cells, chicken embryos, acute lymphoblastic leukaemia, diffuse large b-cell ... the raw sequence reads are filtered based on quality. The adaptor sequences are also trimmed off the raw sequence reads. The ... miRNA sequencing in and of itself is not a new idea, initial methods of sequencing utilized Sanger sequencing methods. ...
The next steps involve extension-based sequencing with cyclic washes of the flow cell with fluorescently labeled nucleotides ( ... however in 2012 the company went bankrupt. There are two main microfluidic systems that are used to sequence DNA; droplet based ... Shotgun sequencing is a sequencing method designed for analysis of DNA sequences longer than 1000 base pairs, up to and ... RNA sequencing was one of the earliest forms of nucleotide sequencing. The major landmark of RNA sequencing is the sequence of ...
The next steps involve extension-based sequencing with cyclic washes of the flow cell with fluorescently labeled nucleotides ( ... PacBio Sales Start to Pick Up as Company Delivers on Product Enhancements , In Sequence , Sequencing , GenomeWeb ... Shotgun sequencing is a sequencing method designed for analysis of DNA sequences longer than 1000 base pairs, up to and ... Main article: Single molecule real time sequencing. SMRT sequencing is based on the sequencing by synthesis approach. The DNA ...
... a high-speed DNA sequencing company that he co-founded), nanopipette-based scanning ion-conductance microscopy, and super- ... in precise delivery of small molecules to cell, and in studying real time detailed cell-functioning. Most recently, his group ... Later, in 2007, this company was acquired by Illumina for $600 million. In 2004, Klenerman co-founded another spin-out company ... He is also known for exploring nanopipette-based (instead of conventional micropipette-based) scanning ion-conductance ...
2 Base Encoding Next-generation sequencing Applied Biosystems Illumina (company) 454 Life Sciences Valouev A, Ichikawa J, ... July 2008). "Stem cell transcriptome profiling via massive-scale mRNA sequencing". Nature Methods. 5 (7): 613-9. doi:10.1038/ ... 0.01/base in 2004 to nearly $0.0001/base in 2006 and increased the sequencing capacity from 1,000,000 bases/machine/day in 2004 ... It uses 2 base encoding to decode the raw data generated by the sequencing platform into sequence data. This method should not ...
... a company later acquired by Illumina. This sequencing method is based on reversible dye-terminators that enable the ... The flow cell has oligonucleotides (short nucleotide sequences) coating the bottom of the cell, and they serve as the solid ... Illumina dye sequencing is a technique used to determine the series of base pairs in DNA, also known as DNA sequencing. The ... "History of Illumina Sequencing". Archived from the original on 12 October 2014. "Illumina - Sequencing and array-based ...
SMRT Cell yield increased up to 50 billion bases for shorter-insert libraries. In April 2019 the company released a new SMRT ... Throughput per SMRT cell is around 500 million bases demonstrated by sequencing results from the CHM1 cell line. On Oct 15, ... It increased average read length to 20,000 bases and half of all reads above 30,000 bases in length. Yield per SMRT Cell ... With the introduction of P6-C4 chemistry typical throughput per SMRT Cell increased to 500 million bases to 1 billion bases. In ...
It consists of coarse oblong granules of yeast, with live yeast cells encapsulated in a thick jacket of dry, dead cells with ... In USA companies like Lesaffre Group, AB Vista, DSM, GB Plange and AB Mauri, produced hundreds of thousands of metric tons of ... It has over 12 million base pairs and around 6000 genes. Since then, it has remained in the forefront of genetic research. For ... In 1996, after 6 years of work, S. cerevisiae became the first eukaryote to have its entire genome sequenced. ...
Hearing of problems with their work, Mullis proposed an alternative technique based on Sanger's DNA sequencing method. ... "Enzymatic Amplification of β-globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia" ... Initially the company screened for microorganisms capable of producing components used in the manufacture of food, chemicals, ... "Direct Cloning and Sequence Analysis of Enzymatically Amplified Genomic Sequences" Science vol. 233, pp. 1076-78 (1986). Saiki ...
"Efficient and sequence-independent replication of DNA containing a third base pair establishes a functional six-letter genetic ... protein and cell membrane synthesis, moving the cell and cell parts (both internally and intercellularly), cell division, etc. ... Roberts & Company. ISBN 978-0-9747077-1-6. Becker S, Feldmann J, Wiedemann S, Okamura H, Schneider C, Iwan K, Crisp A, Rossa M ... The purine bases adenine and guanine and pyrimidine base cytosine occur in both DNA and RNA, while the pyrimidine bases thymine ...
... and single cell sequencing-based investigation of the stem cell plasticity in this model, he aims to understand the molecular ... He is the founder and CEO of the first spin-off company of German Center for Neurodegenerative Diseases, Neuron-D GmbH that ... Developmental Cell. 46 (1): 85-101. doi:10.1016/j.devcel.2018.06.005. PMID 29974866. "1.7 million Euros for young Dresden based ... Cell Reports. 17 (4): 941-948. doi:10.1016/j.celrep.2016.09.075. PMID 27760324. "How fish brain cells react to Alzheimer's ...
Illumina sequencing happens within the flow cells. These flow cells are small in size and are housed in the flow cell ... Illumina's Company Website DNAVision - Illumina official service provider. Gene Logic - performs research based on Illumina's ... The company Grail uses Illumina sequencing technology for tests. The company plans to roll out the tests by 2019, with a cost ... Flow cell clustering happens when a denatured DNA sample is placed in a flow cell. Primers already in the flow cell channel ...
... transcriptomics transitioned to sequencing-based methods. These progressed from Sanger sequencing of Expressed Sequence Tag ... Single-cell RNA sequencing (scRNA-Seq) provides the expression profiles of individual cells. Although it is not possible to ... single molecule direct RNA sequencing has been explored by companies including Helicos (bankrupt), Oxford Nanopore Technologies ... Single cell sequencing can be used to study each cell individually, mitigating this issue. Time dependence: Gene expression ...
... an emotion-sequence animation tool, called FunIcons was created. The Adobe Flash and Java-based application allows users to ... The Neale Publishing Company. pp. 386-7. The Harvard Lampoon, Vol. 112 No. 1, September 16, 1936, pp. 30-31 Gregory Benford, "A ... US 6987991 , for example, discloses a method developed in 2001 to send emoticons over a cell phone using a drop-down menu. The ... Using a lateral click for the nose such as in ( ͡° ͜ʖ ͡°) is believed to originate from the Finnish image-based message board ...
QRM cells complete a sequence of operations ensuring that jobs leave the cell completed and do not need to return. The work ... QRM requires four fundamental structural changes to transform a company organized around cost-based management strategies to a ... relocation of equipment to launch the cell. After cell launching, the implementation team continues support for the new cell ... Resources in a cell are dedicated (only to be used for jobs in the cell), collocated (located in close proximity to each other ...
The Daniell cell - a type of electrochemical cell; an element of an electric battery - invented by John Frederic Daniell (1790- ... Rastrick and Company of Stourbridge, Worcestershire, now West Midlands; the manufacturing company was headed by James Foster ( ... 1820s: Ice hockey, a variant of field hockey, invented by British soldiers based in Canada. British soldiers and emigrants to ... DNA sequencing by chain termination developed by Frederick Sanger (1918-2013). Sanger won the Nobel Prize for Chemistry twice. ...
... is a global biotechnology company based in San Diego, California, US with offices in Maidenhead, UK and Suzhou, China ... Users input a given an amino acid sequence into the web-enabled tool, and the program identifies internal sequences matching a ... Cell. Biol., Mar 2006; 26: 1786 - 1794 Meiluen Yang, Chia-Tse Hsu, Chun-Yuan Ting, Leroy F. Liu, and Jaulang Hwang. Assembly of ... The SUMOplot score system is based on two criteria: first, direct amino acid match to the SUMO-CS observed and shown to bind ...
It is a common human urogenital Mycoplasma species that lacks a cell wall. Due to the absence of the cell wall, M. hominis is ... DNA sequence data is incomplete for M. hominis. M. hominis uses an atypical type of energy metabolism, dependent upon the ... Current therapeutic considerations are based only upon case reports. The bacterium is often passed through sex, so to help keep ... Sternak, p. 1. sfn error: no target: CITEREFSternak (help) "Clindamycin" (PDF). FA Davis Company. 2017. Retrieved 15 December ...
... but is usually based on fixed cells of intervals. He occasionally makes use of twelve-tone sequences and their permutations, ... Theodore Presser Company. Richard Wernick: List of works Kozinn, Allan. "Holding onto a Niche, Very Firmly". The New York Times ... The majority of Wernick's works are published by Theodore Presser Company Most of his manuscripts are held by the Special ... New World Records) CD 762: Lines for Solo Cello Musica Ptolemica for Brass Quintet, Chestnut Brass Company. Albany Records TROY ...
OriV (Origin of Replication): The sequence starting with which the plasmid-DNA will be replicated in the recipient cell. tra- ... There are now many companies that can create a fosmid library from any sample of DNA in a very short period of time at a ... Fosmids are similar to cosmids but are based on the bacterial F-plasmid. The cloning vector is limited, as a host (usually E. ... Conjugation involves using the sex pilus to form a bridge between two bacteria cells; the bridge allows the F+ cell to transfer ...
Large cells can be subdivided into smaller cells for high volume areas. Cell phone companies also use this directional signal ... three cell sites or base transceiver stations. These base stations provide the cell with the network coverage which can be used ... spread spectrum technology developed for military use during World War II and improved during the Cold War into direct-sequence ... The boundaries of the cells can also overlap between adjacent cells and large cells can be divided into smaller cells. The ...
6,258,540, which claims methods of using cell-free fetal DNA (cffDNA) circulating in maternal plasma (cell-free blood) to ... Courts these days are making policy-based decisions, untethered from any rule of law, aimed at killing patents they don't like. ... After Sequenom launched its test on the market, several other companies began to market similar tests and cut prices. Sequenom ... So they wanted to focus on genetic fragments containing paternally inherited sequences the mother did not share, but had ...
BD Cell Pathways "Ingenuity Systems Announces Immediate Availability Of The Ingenuity Pathways Knowledge Base" (Press release ... QIAGEN Silicon Valley (formerly known as Ingenuity Systems, Inc) is a company based in Redwood City, California, USA, to ... Laboratory Corporation and Quest Diagnostics to develop a solution for scoring genetic variation for next generation sequencing ... All QIAGEN Silicon Valley use the Ingenuity Knowledge Base, which contains biological and chemical interactions and functional ...
Chi couldn't find her way home but finds some company led by Tiger. ... The Animatrix (2003, animated sequence). *Hajime no Ippo: Mashiba vs. Kimura (2003) ... Cells at Work! Code Black (2018). *Gurazeni: Pa League-hen (2018). *Cells at Work! Baby (2019) ... Anime series based on manga. *Animated television series about cats. *Comedy anime and manga ...
Statistical, likelihood-based approaches: Statistical, likelihood-based [37][38] iterative expectation-maximization algorithms ... This tracer is a glucose analog that is taken up by glucose-using cells and phosphorylated by hexokinase (whose mitochondrial ... In the United States, a PET scan is estimated to be ~$5,000, and most insurance companies don't pay for routine PET scans after ... Because the two scans can be performed in immediate sequence during the same session, with the patient not changing position ...
... epidermal hair cells (trichomes), cells in the stomatal complex; guard cells and subsidiary cells. The epidermal cells are the ... More than one main vein (nerve) at the base. Lateral secondary veins branching from a point above the base of the leaf. Usually ... Krogh, David (2010), Biology: A Guide to the Natural World (5th ed.), Benjamin-Cummings Publishing Company, p. 463, ISBN 978-0- ... There is a regularity in these angles and they follow the numbers in a Fibonacci sequence: 1/2, 2/3, 3/5, 5/8, 8/13, 13/21, 21/ ...
"The Advisory Board Company. May 24, 2013. Retrieved May 24, 2013.. *^ Barrilleaux, Charles; Rainey, Carlisle (December 11, 2014 ... A sequence of two high-profile studies by a team from the Massachusetts Institute of Technology and the Harvard School of ... Similarly, if a child lives with someone other than a parent, he may still be eligible based on its individual status.[74] ... T-cell count drops below 200).[76] The Medicaid eligibility policy contrasts with the Journal of the American Medical ...
... a base-emitter junction and a base-collector junction, separated by a thin region of semiconductor known as the base region. ( ... W.W Norton & Company Limited. ISBN 978-0-393-31851-7. .. The invention of the transistor & the birth of the information age ... Low operating voltages compatible with batteries of only a few cells.. *Circuits with greater energy efficiency are usually ... A 3-digit sequence number (or one letter then two digits, for industrial types) follows. With early devices this indicated the ...
The arms can be described based on side and sequence position (such as L1, R1, L2, R2) and divided into four pairs.[23][22] The ... The skin consists of a thin outer epidermis with mucous cells and sensory cells, and a connective tissue dermis consisting ... In 2017 a German company made an arm with a soft pneumatically controlled silicone gripper fitted with two rows of suckers. It ... Other colour-changing cells are reflective iridophores and white leucophores.[93] This colour-changing ability is also used to ...
"The Resurrection of Cell and Frieza" / "The Villains Of Hell!! The Revival of Cell and Frieza". Transcription: "Jigoku no ... Unlike the other anime series in the Dragon Ball franchise, Dragon Ball GT is not based on the manga series written by Akira ... She reveals that she was created by the very first wish that Goku and company ever made (Oolong wishing for a pair of underwear ... Later, when searching the brothers' ship for the stolen Dragon Ball, Pan and Giru accidentally trigger the launch sequence, and ...
A new classification and linear sequence based on molecular data can be found in an article by Christenhusz et al.[7] ... The generative cell in the pollen grain divides into two haploid sperm cells by mitosis leading to the development of the ... The Western Maine Forest Nursery Company. pp. 291-295 In Proc. of the North American Forest Tree Nursery Soils Workshop, July ... Then, the first tracheids of the transition zone are formed, where the radial size of cells and thickness of their cell walls ...
The spirochetes may also induce host cells to secrete quinolinic acid, which stimulates the NMDA receptor on nerve cells, which ... Cases have been reported as far east as the island of Newfoundland.[105][214][215][216] A model-based prediction by Leighton et ... revealed the presence of the DNA sequence of Borrelia burgdorferi making him the earliest known human with Lyme disease.[242] ... which was often not reimbursed by insurance companies.[161] Subsequently, hundreds of vaccine recipients reported they had ...
In 2004, at the University of Manchester, researchers Caroline Wilkinson and Fraco Introna reconstructed the saint's face based ... but Christ and the Virgin Mary appear to him in his cell.[48][54] He tells them he is imprisoned "for loving you"[48] and they ... Saint Nicolas on a text by Eric Crozier which covers the saint's legendary life in a dramatic sequence of events. A tenor ... New York: Robert Appleton Company.. *^ Butler, Albin (1860). Lives of the Saints. 2.. ...
Potassium is the major cation (positive ion) inside animal cells,[223] while sodium is the major cation outside animal cells.[ ... organolithium compounds are extremely powerful bases and nucleophiles. For use as bases, butyllithiums are often used and are ... 8).[66] Upon heating of KC8, the elimination of potassium atoms results in the conversion in sequence to KC24, KC36, KC48 and ... "Electrochemical Series", in Handbook of Chemistry and Physics: 92nd Edition (Chemical Rubber Company). ...
LZW is used in GIF images, programs such as PKZIP, and hardware devices such as modems.[8] LZ methods use a table-based ... It (re)uses data from one or more earlier or later frames in a sequence to describe the current frame. Intra-frame coding, on ... such as the coding of speech for digital transmission in cell phone networks). In such applications, the data must be ... almost all the radio stations in the world were using similar technology manufactured by a number of companies. ...
It is represented by a lattice of points which repeats a basic pattern, called a unit cell, in three dimensions. The lattice ... The Moon, with only 63 minerals and 24 elements (based on a much smaller sample) has essentially the same relationship. This ... It is the study of how plants and animals stabilize minerals under biological control, and the sequencing of mineral ... Houghton Mifflin Harcourt Publishing Company. 2017. Retrieved 19 October 2017.. .mw-parser-output cite.citation{font-style: ...
Manitou UK, owner of the American Gehl Company and from Nanterre in France, is based at Verwood on the Ebblake Ind Est off the ... Sunseeker International is a main motor yacht manufacturer; it made the boat in the opening sequence of The World Is Not Enough ... Nearby on Lydiard Fields in Lydiard Tregoze is Johnson Matthey Fuel Cells, which in 2002 was the world's first production site ... BBC South West, based in Plymouth with the Spotlight regional programme.. *BBC West, based in Clifton in Bristol with the ...
Some types of cell adhesion proteins or cell adhesion molecules (CAMs) such as integrins, cadherins, NCAMs, or selectins ... Nelson, D. L., & Cox, M. M. (2008). Principles of Biochemistry (5th ed., p. 377). New York, NY: W.H. Freeman and Company. ... Search integral membrane proteins in the PDB (based on gene ontology classification) ... integral protein from its amino acid sequence and an experimental three-dimensional structure of a related homologous protein. ...
Promoter sequences vary between bacteria and eukaryotes. In eukaryotes, the TATA box is located 25 base pairs upstream of the ... the neighboring sequences can affect if there is a change or not.[30] However, a change can be seen in HeLa cells with a ... Pharmaceutical companies have been designing cancer therapy drugs to target DNA in traditional methods over the years, and have ... Based on the sequence and mechanism of TATA box initiation, mutations such as insertions, deletions, and point mutations to ...
Companies, such as Iogen, POET, and Abengoa, are building refineries that can process biomass and turn it into bioethanol. ... Syngas may be burned directly in internal combustion engines, turbines or high-temperature fuel cells.[27] The wood gas ... This truck is one of 15 based at Walmart's Buckeye, Arizona distribution center that was converted to run on a biofuel made ... Companies, such as Diversa, Novozymes, and Dyadic, are producing enzymes that could enable a cellulosic ethanol future. The ...
Andy passes through a hole in his cell hidden by a movie poster to escape both his cell and ultimately Shawshank.[33] ... based on a survey of 2,120 Hollywood-based entertainment industry members; entertainment lawyers skewed the most towards the ... but with only a single night available to film the sequence, it was shortened to showing Andy standing triumphant in the water. ... having grown up watching him in The Electric Company children's television show.[41] ...
On 16 January 2013, NASA announced that the European Space Agency will build the European Service Module based on its Automated ... distributed throughout the inside of the anthropomorphic phantoms at precise locations of sensitive tissues and high stem cell ... Welding sequence of Orion spacecraft for Artemis 1. This flight will be uncrewed, however, NASA did a study in 2017, at the ... The upper stage ICPS will be based on the Delta Cryogenic Second Stage, consisting of 1 RL10 engine. ...
The specific DNA sequence in the MCM6 gene helps control whether the LCT gene is turned on or off.[19] At least several ... Plant-based "milks" and derivatives such as soy milk, rice milk, almond milk, coconut milk, hazelnut milk, oat milk, hemp milk ... "Bartek, food additive company" (PDF). Archived from the original (PDF) on 2008-12-17. Retrieved 2007-10-11.. ... "Transcriptional heterogeneity in the lactase gene within cell-type is linked to the epigenome". Scientific Reports. 7 (1): ...
She is killed when she and Kiera try to escape the time-travelers' base, but is released by Kiera when she travels back to ... In the episode "Second Wave" it is revealed that Chen is a member of the Freelancer cell from 2077. He is killed by Kiera's ... It was produced by Reunion Pictures, Boy Meets Girl Film Company, and Shaw Media. The plot centres around the conflict between ... Starting with the third season, the narration was replaced by a new sequence that contains a computer-animated version of the ...
... with many genetic testing companies being based in Spain.[28][29]. United KingdomEdit. In the United Kingdom, there were no ... Comparing the DNA sequence of an individual to that of another individual can show whether one of them was derived from the ... The DNA test is performed by collecting buccal (cheek) cells found on the inside of a person's cheek using a buccal or cheek ... Proving a relationship based on comparison of the mitochondrial genome is much easier than that based on the nuclear genome. ...
Main article: Acid-base reaction. A substance can often be classified as an acid or a base. There are several different ... The sequence of steps in which the reorganization of chemical bonds may be taking place in the course of a chemical reaction is ... Instead, these substances are discussed in terms of formula units or unit cells as the smallest repeating structure within the ... Little, Brown and Company. p. 322. ISBN 978-0-316-10930-7. .. ... A third common theory is Lewis acid-base theory, which is based ...
Voxel-based morphometry was used to compare patterns of gray-matter atrophy between patients with and without TD, and a group ... The sequence length increases each trial until the participant is no longer able to replicate the pattern correctly. The test ... Place cells are also found in the hippocampus. The parietal cortex encodes spatial information using an egocentric frame of ... Feltham, Sufolk: Thames Valley Test Company. Olton, D.S.; Samuelson, R.J. (1976). "Remembrance of places past: spatial memory ...
In oats, barley and other cereal grains, they are located primarily in the endosperm cell wall. The oat beta-glucan health ... It is necessary to consider that oats include many varieties, containing various amino acid sequences and showing different ... that allows food companies to make health claims on food labels of foods that contain soluble fiber from whole oats (oat bran, ... "Celiac Disease and Gluten-Free Oats: A Canadian Position Based on a Literature Review". Canadian Journal of Gastroenterology ...
Defense can vary based on the habitat of the bee. In the case of those honey bee species with open combs (e.g., A. dorsata), ... beginning with cleaning out their own cell after eating through their capped brood cell): feed brood, receive nectar, clean ... "Federal Government Seizes Dozens of Misbranded Drug Products: FDA warned company about making medical claims for bee-derived ... inferred from nuclear and mitochondrial DNA sequence data"". Molecular Phylogenetics and Evolution. 40 (1): 315. doi:10.1016/j. ...
W.W. Norton and Company, N.Y.. *^ Jones, D.; et al. (1995). "Sexual selection, physical attractiveness, and facial neoteny: ... in light of some groups using arguments based around neoteny to support racism, Gould also argued "that the whole enterprise of ...
In short, the sequence of events in a scanning spectrophotometer is as follows: *The light source is shone into a monochromator ... It is a known fact that it operates best at the range of 0.2-0.8 O.D. Ink manufacturers, printing companies, textiles vendors, ... Traditional visible region spectrophotometers cannot detect if a colorant or the base material has fluorescence. This can make ... Where aqueous solutions are to be measured, insoluble silver chloride is used to construct the cell. ...
New NEBNext ARTIC SARS-CoV-2 Kits for Multiplexed Amplicon-based Sequencing of Viral RNA. ... New Small Cell Sector Antenna Boasts 0 degree Fixed Electrical Downtilt. * Agricultural & Farming Products ... Company News. Downtime Costs Auto Industry $22k/Minute - Survey. *. Company News. American Excelsior Company Announces $4 ... Can Your Company Help Provide Critical Supplies? We are using the power of our platform to aid in the mass shortage of critical ...
An update to Illuminas gene sequencing technology could have contaminated the results of recent high-sensitivity data produced ... And the average vial of spit that a company like 23andMe might use to sequence your genes contains thousands of cells. "Theres ... the San Diego-based company whose products sequence 90 percent of all genetic data. ... because he was sequencing so many cells with so little genetic material to work with. A typical mammalian cell contains only ...
Blom N, Gammeltoft S, Brunak S (1999). "Sequence and structure-based prediction of eukaryotic protein phosphorylation sites". ... PB-CD4+ T cells, PB-CD8+ T cells, lymph node, lung, thymus, thyroid, leukemia chronic myelogenous K562, and leukemia ... "SUMOplot Analysis Program". Abgent- a WuXi AppTec Company. Retrieved 14 May 2013. Johansen MB, Kiemer L, Brunak S (2006). " ... There is also an alternate form of CCDC130 where only 803 bases are transcribed instead of 1433 bases, but there is no ...
... solar cells, with product certification to begin in mid-2019, according to company president Doris Hsu. ... Atgenomix provides subscription-based genome sequencing analysis services. 1h 43min ago in IT + CE ... SAS unveils N-type solar cells (Jun 28). *SAS recognizes provision of NT$4.35 billion for polysilicon onerous contract (Jun 27) ... Sino-American Silicon Products (SAS) is preparing for production of N-type TOPCon (tunnel oxide passivated contact) solar cells ...
News on sequencing-based testing, NGS gene panels, & diagnostic exomes in genetics, genomics, and molecular diagnostics. from ... The immune cell sequencing company beat Wall Street estimates on the top line, but missed on the bottom line for both Q4 and ... The Chinese company will use the funds to accelerate the registration and commercialization of its NGS-based products for ... News on sequencing-based testing, NGS gene panels, & diagnostic exomes in genetics, genomics, and molecular diagnostics. ...
The potential vaccines are based on Corixas sequencing of human genes from lung cancer cells. ... 3) US biotech company Corixa is based in Seattle. See Corixa press release, 16 June 1999, and Corixa 1999 Annual Report [no ... The patents include claims for human gene sequences from lung cancer cells (5). If granted, the gene patents will allow Corixa ... Governments must end the patenting of genes and stop biotech companies like Corixa and Cell Genesys riding roughshod over ...
The second company, Inovio Pharmaceuticals, based outside Philadelphia, uses vaccines based on inserting virus sequence into ... The Moderna vaccine works by converting genetic sequences of the virus into RNA which when injected into the body, causes cells ... The company can use genetic sequence of the virus that China released to get started on its vaccine, and doesnt need a sample ... Based on past experience, though, the chances of all that falling into place fast enough to turn the tide arent great, many ...
Two companies offering droplet-based single-cell RNA sequencing sample prep, 10x Genomics and 1Cellbio, have launched dual- ... Pediatric Ependymoma Single-Cell Analysis Points to Signatures Linked to Outcomes. The single-cell RNA-sequencing analysis ... Childhood Ependymoma Single-Cell Sequencing Study Reveals New Cellular Subpopulations. Using 26 pediatric ependymoma patient ... These hybrid capture-based methods target gene transcripts of interest, which could shrink sequencing costs for some ...
... life sciences researchers talk about the obvious solution-cell-line authentication-but they fail to implement it. Maybe they ... The most recent trend in cell-line authentication is the move to DNA sequencing techniques over earlier methods based on ... They will have confidence that their cell lines "are what they think they are." Also, best-in-class companies may come to ... "While sophisticated phenotype-based systems exist for in-depth metabolic analyses of cell-lines, simple and rapid ...
Large-scale screening based on this is promising. ... Large-scale screening based on this is promising. ... although it has no effect on mouse tumor cells and normal human cells. The mechanism studies showed that these siRNAs could ... although it has no effect on mouse tumor cells and normal human cells. The mechanism studies showed that these siRNAs could ... They also significantly inhibited the growth of human tumor cells in vivo. 17 siRNAs were designed for seven more mouse- ...
Early Access Program for WaferGens single cell analysis technology ongoing; initial data expected in third quarter Established ... MD Anderson Cancer Center in Houston, TX, as the Companys fourth collaborator... ... In addition, WaferGen has established proof-of-concept for its single cell technology, which is based on the Companys ... faster and cheaper genetic analysis based on Next-Gen Sequencing and Real-Time PCR. ...
... patents and companies. The markets for sequencing products and services are given for the years 2012, 2013 and 2018.This report ... also reviews the main sequencing platforms, and explains why genetic variation is important for many markets, including ... This report includes sequencing technologies, applications, industries, initiatives, markets, ... single cell genomics; NGS-based proteomics; informatics; information technology; clinical laboratory; and the prenatal ...
Real-Time Sequencing was featured in a number of presentations during last weeks Advances in Genome Biology& Technology ... RS II Sequencing System, today announced that its Single Molecule, ... Based on its novel Single Molecule, Real-Time (SMRT®) technology, the companys products enable: de novo genome assembly to ... and find novel genes in the most comprehensively studied human cell lines; targeted sequencing to more comprehensively ...
... a leading global medical technology company, and Cellular Research, an innovative company focused on enabling high-resolution ... announced that they will be jointly promoting their unique solutions for highly multiplexed cell isolation and single-cell gene ... single-cell sorting instrumentation and software with Cellular Researchs Precise™ assays based on Molecular Indexing™ ... BD and Cellular Research Announce Co-marketing Efforts for Single-Cell Sequencing * Standardized Incubation and Imaging System ...
... sequencing, data analysis and cell-based assays. Experiences in hybridoma/B cell cloning, NGS, automation, ELISA, Biacore SPR, ... Highly motivated, willing to work in a startup company, eager to learn/develop protein chemistry and molecular cell biology ... Amberstone Biosciences Inc is a startup company located in Orange County, Southern California. Our proprietary single cell ... Highly motivated, willing to work in a startup company, eager to learn/develop molecular cell biology techniques. ...
But the incubator model is beginning to change those statistics for companies who seek the right kind of help. ... a Houston-based biotechnology company commercializing next-generation DNA sequencing chemistry. The company has recently ... Founded in San Francisco in 2005, Fluxion Biosciences manufactures analytical instruments for functional cell-based assays and ... Client Base:. 35 companies. Client Composition:. Life science: 75%;. Materials science: 15%. Clean technology: 10%. Other: 10% ...
Cell-based protein assays. Living cell-based assays for protein function. Companies developing cell-based protein assays ... Bottom-up protein sequencing. Top-down protein sequencing. Integration of next generation proteomics and gene sequencing data. ... Table 2-3: Companies supplying mass spectrometry instruments. Table 2-4: Companies involved in cell-based protein assays. Table ... Cell-based assays for GPCR. Companies involved in GPCR-based drug discovery. GPCR localization database. Matrix ...
... that the companys next-generation sequencing-based approach to identification and quantification of populations of B cells can ... Adaptive Biotechnologies Corporation is a platform-based, diagnostic-driven company that leverages NGS to profile T-Cell and B- ... In the first step, the clonoSEQ ID test, cancer cell DNA sequences are identified in a diagnostic sample. In the second step, ... Adaptive Biotechnologies clonoSEQ process enables physicians to utilize sequencing-based minimal residual disease (MRD) ...
I direct an HLA lab that completes HLA sequence-based typing for clinical transplantation and for research studies of cancer ... He is the scientific founder and Chief Scientist at the Pure MHC and Pure Protein biotech companies. Dr. Hildebrand received ... how HLA molecules distinguish cancerous cells to facilitate the immune recognition of tumors, and the role of HLA molecules in ... She continues to lead policy change for MAC as the project lead for the socio-economic disparities work group based on her idea ...
DNA Sequencing, Nanobiotechnology, Tissue engineering and Regeneration, Fermentation, Cell Based Assay, PCR Technology, ... annual reports of the companies, journals, and others and were checked and validated by the industry experts. The facts and ... This Biotech Transports Cell Therapies Without Needing a Freezer. June 21, 2019. ... Reports is an upscale platform to help key personnel in the business world in strategizing and taking visionary decisions based ...
... cell-based therapy, RNA therapeutics, next-generation sequencing, and medical devices. She has served a variety of clients, ... including independent inventors, startup biotechnology companies, global pharmaceutical companies, academic institutions, and ... She applies her bilingual language skills to assisting cross-border matters involving Chinese companies. ... she conducted genome-wide RNAi screens and developed a computational pipeline for next-generation sequencing analysis to ...
Scientists first converted polioviruss published RNA sequence, 7741 bases long, into a DNA sequence, as DNA was easier to ... The mechanism of viral release from the cell is unclear, but each dying cell can release up to 10,000 polio virions. Drake ... The complete viral genome was then assembled by a gene synthesis company. Nineteen markers were incorporated into the ... Mueller S, Wimmer E (August 2003). "Recruitment of nectin-3 to cell-cell junctions through trans-heterophilic interaction with ...
7741 bases long, into a DNA sequence, as DNA was easier to synthesize. Short fragments of this DNA sequence were obtained by ... Follicular dendritic cell sarcoma. Extranodal NK/T-cell lymphoma, nasal type. MCPyV Merkel-cell carcinoma. RNA virus. HCV ... The complete viral genome was then assembled by a gene synthesis company. This whole painstaking process took two years. ... The mechanism of viral release from the cell is unclear,[2] but each dying cell can release up to 10,000 polio virions.[26] ...
... tiny genetic missteps that can make cells grow out of control or become resistant to certain medicines. Identifying those ... The DNA of cancer cells is littered with mutations - ... a sequencing company based in Branford, CT. Amplified DNA from ... They first amplified the tumor cell DNA from a specific gene - the epidermal growth factor receptor (EGFR) - and then sequenced ... The outfit is one of several companies striving to create fast, accurate, and affordable sequencing methods, which could ...
"In the post genome sequence era, very broad DNA patients based on newly discovered sequence, like the patents Myriad filed in ... Another company, GeneDx, plans to offer a 27-gene panel for breast and ovarian cancers, as well as next-generation sequencing- ... based multigene panels for gastrointestinal and colorectal cancers, pancreatic cancer, endometrial cancer, and renal cell ... a division of Houston-based genomics and genetics testing company Gene By Gene, Ltd, reported that it would be offering testing ...
Company Description. We have developed a high-sensitivity, next-generation sequencing-based system for the the detection of ... Potency Assay Development for Cell and Gene Therapy Products. Create well-controlled, biologically relevant test systems and ... cancer mutations in miniscule concentrations of circulating cell-free DNA (cfDNA) in blood. ...
... next-generation sequencing, synthetic biology, genomics, cloning and nucleic acid based detection. Oligonucleotides synthesis ... Cells Powered by Magnetic Nanoparticles Help Heal Post Infarct Hearts. Nanoparticles Laden with Tapeworm Drug Stop Activity of ... To support companies in overcoming complex business challenges, we follow a multi-disciplinary approach. At PMR, we unite ... In addition, rising field of next-generation sequencing to increase demand for next generation sequencing-grade oligos would ...
RNA sequencing technology, an innovative multifaceted single-cell sequencing platform for simultaneous protein and RNA ... for Enhanced Single Cell Analysis with Protein Expression (ESCAPETM) ... Advantages of the ESCAPETM sequencing platform include: * Single cell analysis * Phenotype cells based on known cell markers * ... "We are excited to offer this technology to the wider community of clinical researchers, biotechnology and pharma companies. We ...
Korro Bio is a privately held biotechnology company launching out of a top-tier life science venture firm headquartered in ... We are utilizing the precision provided by nucleic acid-based therapeutics to target the sequences responsible within the ... Participate in the development and scaling of novel cell based assays. *Deployment of cell-based models and assays for drug ... Broad experience in the application of biochemical, biophysical & cell-based techniques such as mammalian cell culture, ...
The tool returns suggested RNA sequences based on predicted specificity, efficiency, and map position. Users can adjust the ... including feeder and support cells, cancer cell lines, hybridomas, and primary cells. Human and mouse stem cells available for ... These free protocols are contributed by academic research labs, biotechnology companies, and scientific community-based ... Nucleotide sequences, including genomic DNA, mRNA, and noncoding RNA sequences may be deposited into GenBank. Sequences in ...
  • Precision medicine-like spotting a piece of tumor DNA in a drop of blood or finding a rare variant among the 3 billion base pairs in the human genome 1 -also requires high-resolution sequencing. (
  • The sequencing of the human genome has revealed that only ~1.2% of the human genome encodes for protein-coding genes, yet the large majority of the human genome is transcribed into non-protein-coding RNAs (ncRNAs) ( 1 , 2 ). (
  • MENLO PARK, Calif., March 2, 2015 (GLOBE NEWSWIRE) -- Pacific Biosciences of California, Inc., (Nasdaq:PACB) provider of the PacBio ® RS II Sequencing System, today announced that its Single Molecule, Real-Time (SMRT ® ) Sequencing was featured in a number of presentations during last week's Advances in Genome Biology & Technology (AGBT) conference, including demonstrations of the technology's ability to create reference-quality de novo human genome assemblies. (
  • At the company's workshop, J. Craig Venter, Ph.D., of Human Longevity, Inc. presented data about his highly studied genome, which has now been sequenced using the PacBio RS II and assembled in the cloud on the DNAnexus platform, creating a higher resolution version of this reference genome at a fraction of the original time and cost. (
  • Deanna Church, Ph.D., who has played a key role in the public efforts to create a human reference genome, discussed the importance of having more high-quality de novo human genomes, and Gene Myers, Ph.D., from the Max Planck Institute discussed his work to develop computational methods to enable perfect assemblies using SMRT Sequencing data. (
  • In addition, W. Richard McCombie from Cold Spring Harbor presented analysis of structural re-arrangements and gene amplifications in a breast cancer cell line genome. (
  • With the performance improvements planned for this year, we expect the cost to generate a human genome on the PacBio RS II to drop to around $10,000, which is not a high premium to pay for the superior quality and completeness that SMRT Sequencing provides. (
  • Evan Eichler, Ph.D., from the University of Washington presented more data about his work on characterizing complex variation in the human genome using SMRT Sequencing. (
  • In addition, the Genome Reference Consortium presented de novo assemblies for two human cell lines targeted for "platinum-grade" references. (
  • Jonas Korlach, Ph.D., Chief Scientific Officer of Pacific Biosciences, added: "We are excited to see how our customers are using SMRT Sequencing for an increasing number of important human and other complex genome studies, including characterizing variation beyond SNPs, developing population-specific genome references, and resolving the genetic basis of disease. (
  • As a postdoctoral fellow at Massachusetts General Hospital, she conducted genome-wide RNAi screens and developed a computational pipeline for next-generation sequencing analysis to discover genetic regulators of aging. (
  • Thus, the genome enclosed within the viral particle can be used as messenger RNA and immediately translated by the host cell. (
  • The right to control exclusively the use of a patient's genes could have made it more difficult to access new tests and treatments that rely on novel technologies that can quickly determine the sequence of any of the estimated 20,000 genes in the human genome," he said. (
  • Companies are vying to stake out patent-protected territory in what they believe will be the therapeutic battlefield of the 21st Century: the human genome. (
  • 1 The Human Genome Project, (HGP) an international public consortium of research laboratories led by the United States, and Celera, a private American company, made the announcement jointly, ending months of competition to complete the first sequence of the human genome. (
  • The human genome has been sequenced separately and independently with major public finance, from the United States and the European Community. (
  • But that has not prevented the human genome from being owned and exploited by private companies. (
  • Celera kept its own human genome data secret while benefiting from free access to the public database throughout the period that the company was busy sequencing, thereby significantly reducing the time and effort needed to complete the task. (
  • The US and European Governments, in line with private companies, are downplaying the free access to the public human genome database on grounds that raw genome sequence is useless. (
  • 5 This seems like a definite improvement over the previous situation in the United States where over 4 million patents on human genome sequences have already been granted 6 , the majority of which are on short fragments of DNA with no known function. (
  • Genome sequencing/mapping. (
  • The global single-cell genome sequencing market size is expected to reach USD 2.49 billion by 2025, registering a CAGR of 14.64% during the forecast period. (
  • Earlier, single-cell genome sequencing market was dominated by PCR and microarray technologies. (
  • Furthermore, rising number of single-cell genome sequencing centers & associated facilities worldwide is fostering growth prospects. (
  • It's simpler than sequencing a whole genome: By looking at conserved gene regions that have mutated throughout evolutionary history, scientists can identify a species based on relatively few segments of DNA. (
  • Adaptive Biotechnologies, a Fred Hutch spinoff , was founded on the idea that while the 6 billion letters of DNA that make up a human genome are consistent in almost every cell of the body, the immune system's B cells and T cells are an exception. (
  • Their new technology, called INTEGRATE, harnesses bacterial jumping genes to reliably insert any DNA sequence into the genome without cutting DNA. (
  • Rather than introduce DNA breaks and rely on the cell to repair the break, INTEGRATE directly inserts a user-defined DNA sequence at a precise location in the genome, a capability that molecular biologists have sought for decades," says Sternberg, who was recently recruited to Columbia from Jennifer Doudna's laboratory at UC Berkeley. (
  • Typically, researchers want to make a small change at one specific sequence of DNA bases, leaving the rest of the genome untouched. (
  • The researchers harnessed this discovery to create a gene-editing tool that can be programed to insert any DNA sequence into any site in a bacterial genome. (
  • And by replacing the transposon sequence with other DNA payloads, they could insert sequences up to 10,000 bases long into a bacterial genome. (
  • However, a whole genome shotgun approach has been used to sequence the mouse, fly and human genomes by the private company Celera. (
  • This involves shotgun cloning the whole genome and sequencing the clones without creating a physical map. (
  • For example, a part of the fly genome was sequenced by the one-by-one approach and the whole genome shotgun method. (
  • The whole genome shotgun method generated the sequence much more quickly, but the one-by-one approach is probably more accurate because the genes were studied in more detail. (
  • These mutations were introduced in a non-evolved reference strain using CRISPR/Cas9-based genome editing. (
  • Identification of mutations responsible for these fast-growing phenotypes by whole-genome sequencing and reverse engineering showed that, for each compound, a small number of mutations sufficed to achieve fast growth in its absence. (
  • The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species. (
  • After revolutionizing genome sequencing, NGS is expected to also markedly change the diagnostic market. (
  • The tiny shears are combined with an RNA-based targeting molecule that scans the genome for specific sequences and makes a controlled cut at a single site. (
  • When healthcare professionals have not been able to identify the cause of a baby's ill health, whole genome or exome sequencing offers a way of searching simultaneously across large parts of the genetic code for genetic causes of disease. (
  • Parents of babies in the UK and elsewhere might be able to access whole genome and exome sequencing through commercial providers in the future. (
  • Whole genome sequencing is already available to adults through several US-based companies. (
  • Some suggest that the programme should be expanded to include more childhood conditions, and that using whole genome or exome sequencing could become a cost effective way to achieve this. (
  • The usefulness and implications of whole genome sequencing of newborns is being explored in a US$25 million study funded by the National Institutes of Health in the US. (
  • Many of the strides made in genetic diagnostics are direct results of the Human Genome Project, an international thirteen-year effort begun in 1990 by the U.S. Department of Energy and the National Institutes of Health, which mapped and sequenced the human genome in its entirety. (
  • Performing a DNA sequencing experiment (Genome Sequencer FLX): an automated, ultra-fast tool for DNA sequencing based on innovative microtechnology methods. (
  • A new startup, backed with $43 million in venture investments, aims to develop treatments that could cure inherited diseases with a one-time fix based on a new method of genome editing. (
  • The method offers great precision in changing the DNA sequence of a genome and can potentially treat diseases that other forms of gene therapy cannot. (
  • The new method, to be commercialized by Editas Medicine , is based on a genome-editing system that was largely unknown just two years ago. (
  • Researchers have adapted this bacterial immune system, referred to as CRISPR/Cas, to edit single base pairs of the human genome as well as larger stretches of DNA. (
  • Current broad-based sequencing techniques can fail to catch the genetic variations that comprise a tumor, and whole genome sequencing is far too time and cost consuming to be implemented at scale," Silver explained. (
  • That little sample of saliva may not seem like much, but it holds a few of your cheek cells, each of which contains your entire genome-all the DNA that makes you, you. (
  • Those chemicals, called bases, form pairs: A pairs with T, C with G. Every person has three billion of those pairs in their genome, and the order in which those pairs line up one after another acts as a blueprint to build that unique individual. (
  • While the $10,000 cost of sequencing a genome makes it prohibitive for most people and organisations, an alternative-known as genotyping-is already available from companies such as 23andMe and Personalis Genomics, at a far lower cost. (
  • As one of the first research Institutes to take part in the MinION Access Programme (MAP) for portable DNA sequencing, introduced by Oxford Nanopore Technologies, The Genome Analysis Centre (TGAC)'s task force share their experience of the ground breaking trial so far. (
  • Epigenetic sequencing detects the location of the epigenetic markers across the genome. (
  • The statements are made in reference to the work of Dr. Craig Venter and colleagues at the J. Craig Venter Institute ( JCVI ) when the team announced the success of efforts to replace the chromosome of a bacterial cell with a "chemically synthesized genome" (Gibson, 2010). (
  • First, the DNA sequences that encode the genome of the bacterium Mycoplasma mycoides were manipulated on a computer. (
  • By comparison, there are three billion base pairs of DNA in one copy-23 chromosomes-of the human genome). (
  • It" is the Human Genome Project, a multiyear, multibillion-dollar federally backed research project to identify the location of every chromosome, gene and base pair of deoxyribonucleic acid (DNA) -- all three billion base pairs, in fact -- that make up the human cell. (
  • Life Tech recently signed a three-year contract with Los Alamos National Laboratory that the company hopes will make it a central player both in the Genome Project and in much of the years of research that flow from it. (
  • Last month, Venter and his company, Human Genome Sciences of Rockville, Maryland, announced that any researcher who wants to use the information for academic reasons can do so, free of charge. (
  • This means identifying the sequence of chemicals - or bases - that make up the genome. (
  • With this method, Venter realised he could locate any gene the body expresses in a fraction of the time it would take to find it by sequencing the entire genome. (
  • Prequel's performance is based on the whole genome sequencing (WGS) approach, which identifies chromosomal irregularities like trisomy 21 (T21), trisomy 18 (T18) and trisomy 13 (T13). (
  • The licensed technology is a modified measles virus based on the viral genome sequence of the established measles vaccine strain, which has been used to immunize billions of people worldwide. (
  • MENLO PARK, Calif.--( BUSINESS WIRE )--GRAIL, Inc., a healthcare company focused on the early detection of cancer, today announced that new data from the Circulating Cell-free Genome Atlas (CCGA) Study will be presented during the 2018 American Society of Clinical Oncology (ASCO) Annual Meeting taking place June 1-5 in Chicago. (
  • Sensitivity analyses were conducted by sequencing blood samples from 878 participants with newly diagnosed cancer with three prototype genome sequencing assays. (
  • Results for the whole-genome bisulfite sequencing assay are reported in this press release. (
  • In this initial discovery phase of CCGA, three prototype genome sequencing assays were used to evaluate cancer-defining features in cell-free nucleic acids. (
  • Detection rates (sensitivity at 98 percent specificity) with the whole-genome bisulfite sequencing assay at stages I-III for cancer types with strong blood-based biological signal, are detailed in the table below. (
  • Gene expression had revealed 41 distinct subpopulations of blood-forming stem cells, including a group of cells that seemed capable of transitioning into any of the other ones-his true stem cell. (
  • The sequencing test involves a single library preparation that is then split for parallel analysis of promoter methylation, MSI calculation, and gene mutation detection. (
  • The patents include claims for human gene sequences from lung cancer cells (5). (
  • If granted, the gene patents will allow Corixa to prevent other companies and researchers from developing or marketing other methods of diagnosing, preventing or treating lung cancer which rely on these genes for at least 20 years. (
  • A team of researchers in Germany investigated gene expression in airway cells of SARS-CoV-2 patients, finding increased interaction between epithelial and immune cells. (
  • SAN JOSE, CA, Feb. 26, 2015 - BD Life Sciences, a segment of BD (Becton, Dickinson and Company) (NYSE: BDX), a leading global medical technology company, and Cellular Research, an innovative company focused on enabling high-resolution biology, today announced that they will be jointly promoting their unique solutions for highly multiplexed cell isolation and single-cell gene expression analysis. (
  • The efficiency of identifying and isolating single cells greatly increases the throughput of cells available for transcriptome analysis, creating the potential, now within reach of investigators, to analyze gene expression targets in many thousands of individual cells. (
  • In a paper published online this week in Nature Medicine , scientists at the Dana-Farber Cancer Institute and Broad Institute of MIT and Harvard showed that a new type of sequencing technology, known as "parallel picoliter reactor sequencing," could identify mutations in a gene targeted by lung cancer drugs, while traditional sequencing technologies could not. (
  • They first amplified the tumor cell DNA from a specific gene - the epidermal growth factor receptor (EGFR) - and then sequenced the gene using technology developed by 454 Life Sciences , a sequencing company based in Branford, CT. (
  • Traditional sequencing methods would generate the sequence of the region containing the gene from this soup of DNA. (
  • The key is to be able to sequence a gene from a single DNA molecule. (
  • Francis S. Collins, MD, PhD, director of the National Institutes of Health, issued a statement shortly after the ruling, saying that he was pleased with the decision and that it "represents a victory for all those eagerly awaiting more individualized, gene-based approaches to medical care. (
  • In fact, on the same day as the Supreme Court announced its decision, DNATraits, a division of Houston-based genomics and genetics testing company Gene By Gene, Ltd, reported that it would be offering testing for the BRCA1 and BRCA2 genes for $995. (
  • Despite international controversy over the patenting of human genes and other life forms, US-based genomic companies (in partnership with major pharmaceutical corporations), are filing patents for exclusive monopoly control on human genes and gene fragments. (
  • In this issue, RAFI takes a look at the astonishing growth of new companies, popularly known as the gene boutiques," that are devoted to identification, characterization and commercialization of human genes and their products. (
  • U.S.-based genomic companies are applying for patents covering thousands of human gene sequences. (
  • What is Gene Sequencing? (
  • Information in DNA is encoded in the form of four nucleotides designated A,C, G, T. The order of the nucleotides, is called the DNA sequence of the gene, and it determines the order of the amino acids that make up the protein product of the gene. (
  • The DNA sequence of a gene accurately predicts the amino acid sequence of the protein. (
  • 2) Genomic companies hope to use gene sequencing to ultimately discover and understand the so-called "disease-causing" genes, and to develop diagnostic and treatment products. (
  • New genomic companies vary greatly in their precise strategies for gene discovery, but most specialize in gene mapping, DNA sequencing, and bioinformatics (computational molecular biology). (
  • Although most of the genomic companies have yet to market a single product, they are filing patent claims on genes and gene fragments, as well as related genomic technologies at a feverish pace. (
  • In the weeks following, officials in the Clinton administration clarified that they still favored patents on "new gene-based health care products. (
  • In contrast, the gene sequences and gene maps produced by the public consortium have been deposited regularly within 24 hours of completion in GenBank, a public database set up in the early 1980s when DNA sequencing began, access to which is totally free. (
  • There are some 20,000 patents on gene sequences pending at the US patent office. (
  • Gene sequencing/mapping. (
  • If we learn about that gene by random sampling, by using 50 million total sequences, which is what SBS provides, we can characterize that gene at depth, he noted. (
  • The company owns a gene-editing technology based on zinc-finger proteins. (
  • The company insists it can permanently switch on, or off, the expression of any gene. (
  • In the laboratory, Sangamo has shown its compound can enter CD34 stem cells of patients with either beta-thalassemia or sickle cell and permanently knock out their BCL11A gene. (
  • But aside from single-gene knockouts to allow for metabolic selection systems, the Chinese hamster ovary (CHO) host cell line remains largely unchanged from what was used 30 years ago. (
  • Two: I have no idea what the $99 23andme test covers with regard to BRCA1 and BRCA2 gene mutations beyond what the company states . (
  • Some are offended by the very notion that a private company can own a patent based on a gene that was invented not by researchers in a lab but by Mother Nature. (
  • By measuring gene activity in iPS cells against the study's gene expression range, Meissner's lab was able to accurately score cells for their potential to differentiate into particular cell lineages. (
  • Today, flow cytometry and PCR - along with highly sensitive next-generation gene sequencing - have gained "solid ground" in clinical practice and have become the "gold standard" for assessing molecular remission, Rai said. (
  • One standard barcode region, for example, is a sequence of 648 base pairs within the gene for mitochondrial cytochrome c oxidase 1, or CO1. (
  • Every animal contains a version of the gene for CO1, a critical part of the electron transport chain that helps drive the synthesis of ATP-the fuel that runs your cells. (
  • This makes gene editing difficult or impossible in some cell types, and severely constrains researchers' ability to introduce precise genetic modifications in a safe manner. (
  • Sequence-based analysis enabled by the 3500 Dx system coupled with SeCore kits is the only available technique that identifies more than 5500 gene variants at the nucleotide level, resulting in the most accurate and unambiguous assay. (
  • It is faster and cheaper than creating a physical gene map and sequencing clones one by one, but the reliability of reassembling all the sequences of the small fragments into one genomic sequence has been doubted. (
  • The sequencing technology simultaneously records the tens or hundreds of thousands of gene transcripts of many thousands of cells. (
  • This new improved PCR method is based on the gene sequence of a protein discovered in a sub-fraction of cell-free culture broth from isolates of V. parhaemolyticus that cause AHPND. (
  • Primer (AP3) was designed to amplify the gene sequence of one small protein from this toxic sub-fraction. (
  • This peptide with the sequence 5' - GLNDIFEAQKIEWHE - 3' contains a central lysine that is specifically biotinylated by the prokaryotic enzyme biotin holenzyme synthetase, encoded in the BirA gene of E. coli. (
  • Specific biotinylation of this peptide sequence can be performed in vivo by contransfecting a plasmid with the BirA gene as described for the AAV in Arnold et al. (
  • If approved, this test could be the first FDA-approved multi-gene sequencing-based in-vitro diagnostic (IVD) to accept both plasma and FFPE-based inputs. (
  • Indee Labs received US$2.6M in venture financing extending its seed-stage funding to more than US$5.4M (AU$7.1M). This will accelerate the development of Indee Labs' gene therapy manufacturing technology into a product that biotechnology companies can use for discovery-stage research. (
  • All of the industry excitement around discovering gene targets for cell therapy has overlooked an inconvenient fact. (
  • The team at Indee Labs has developed a gene delivery technique that is not only inexpensive enough for general use, but fast enough to use the patient's own cells. (
  • This has been supported by transcriptome-wide sequencing data, which showed negligible differential gene expression in over 200,000 unique transcript reads, along with additional studies that revealed no variation in cytokine production or activation phenotype for processed cells relative to the handling controls," explained Chief Scientific Officer, Amy Twite PhD. (
  • Harvard geneticist George Church , who cofounded Editas, says the technology's ability to change single base pairs enables fundamentally new ways of thinking about gene therapy. (
  • With European approval of a gene therapy in 2012 (" Gene Therapy on the Mend as Treatment Gets Western Approval ") and the launch of new gene therapy companies (" Improved Virus Technology Spurs New Gene Therapy Startups "), the idea that inherited diseases could be cured with genetic medicines seems to be dusting off its precarious start. (
  • Generally, the treatments involve adding a functional copy of a gene to a patient's cells. (
  • Traditional methods of gene therapy cannot address this disease, but CRISPR/Cas has the potential to correct the faulty DNA sequence. (
  • Another advantage of the gene-editing potential of CRISPR/Cas technology is that the corrected gene remains in its normal chromosome location, which preserves the way the cell normally turns a gene on or off. (
  • The proprietary platform produces oligomers, now known as "ONCOlogues," that are capable of binding gene sequences 1,000 times more effectively than complementary native DNA. (
  • Scientific Co-Founder Jeffrey Rothman, M.D., Ph.D., said, "Through rigorous statistical, mechanical and molecular modeling, combined with gene sequence data, we are able to create sequence-specific, targeted therapeutics against oncogenes, which are the cause of and specific to tumor cells. (
  • The San Francisco-based Mission Bio's technology, called the Tapestri Platform, is used by researchers, hospitals and clinicians to detect disease progression and treatment response, inform drug discovery, and verify gene edits. (
  • The Bedford, Mass., firm is building a new type of gene-editing tool based on the research of its founder, Saswati Chatterjee, a professor of Virology at Beckman Research Institute. (
  • Sequencing DNA is the scientific term for figuring out the order in which different base pairs of DNA are arrayed on a gene. (
  • For example, scientists think that the genetic damage that causes muscular dystrophy is caused by the fact that the base pairs on a single gene are in the wrong sequence, he said. (
  • This external stimulus turns the gene on and off and affects the ability of the cell to read the gene. (
  • For example, DNA methylation and histone modification are posterior processes such as altered gene expression that is not observed in the DNA sequence. (
  • Drugs based on epigenetics are reported to be incredibly effective and aid reverse abnormal gene expression. (
  • A German research team led by Ingo Kurth from the Institute of Human Genetics at the University Hospital Jena, Germany, used a number of different methods, including Roche's NimbleGen Custom Sequence Capture 385K array to identify the gene mutated in the disease locus of the X-chromosome of a Spanish family with hereditary hearing loss 1 . (
  • Bioinformatic analysis of gene and protein patterns detected in tumor cells and peripheral blood is proving to be a big breakthrough in cancer diagnosis. (
  • And thanks to the development of highly specific gene-amplification and sequencing technologies LBs access more biomarkers relevant to more cancers than ever before. (
  • Two companies offering droplet-based single-cell RNA sequencing sample prep, 10x Genomics and 1Cellbio, have launched dual-indexed library preparation products. (
  • Oligonucleotides also have its application in nucleic acid array based technology, diagnostics, genetic engineering, next-generation sequencing, synthetic biology, genomics, cloning and nucleic acid based detection. (
  • Stem cell research and genomics have rapidly advanced in parallel over the past few years," said Dr. Meissner. (
  • Vice President and General Manager of Life Technologies Fragment and Sequence Genomics Division. (
  • The funding supports collaborations between Fred Hutch's Dr. Jason Bielas and the company 10x Genomics to develop a droplet-based, single-cell RNA sequencing platform. (
  • Mission Bio is a precision genomics company that which has applied its technology to drive cancer research. (
  • The team of scientists from TGAC's Data Infrastructure and Algorithms and Plant and Microbial Genomics groups trialled the miniaturised sensing system by sequencing environmental samples, containing DNA from hundreds or thousands of different organisms. (
  • Subsequent sequencing analysis at the Cologne Center for Genomics (CCG) resulted in the identification of a total of 3858 and 3443 X-chromosomal variants for each of these two individuals. (
  • The system of granting patents on genes underpins one of these exclusive deals, allowing companies to seize genetic information and sell it to the highest bidder. (
  • Governments must end the patenting of genes and stop biotech companies like Corixa and Cell Genesys riding roughshod over patients' interests. (
  • The potential vaccines are based on Corixa's sequencing of human genes from lung cancer cells. (
  • Researchers have developed a method, called MemorySeq, to identify genes transcriptome-wide whose expression fluctuations persist for several cell divisions. (
  • Many companies believe that information flowing from the discovery of human genes and their protein products will revolutionize pharmaceutical drug development, provide a new set of drug discovery tools, and novel therapies for treating human diseases. (
  • Genomic companies limit their search to relatively small chromosomal regions that contain genes of suspected medical or biological importance--functional DNA. (
  • Using a novel deep sequencing technology that can in one fell swoop decode 50 million sequences representing well over a billion bases of DNA, a research team led by University of Delaware scientists is working to unmask where, why and how certain genes are switched on or off in rice--a crop vital to the world's food supply. (
  • A screen to identify essential genes and those that improve cell fitness was used as a proof-of-concept to validate the CRISPR screening conditions. (
  • Every single cell in every single person has copies of the BRCA1 and BRCA2 genes. (
  • Dr. Meissner is the lead author of a study in the journal Cell (Vol. 144, No. 3, pp. 439-452, Feb. 4, 2011) that identified a range of expression levels among key genes associated with pluripotency. (
  • DNA sequencing may be used to determine the sequence of individual genes , larger genetic regions (i.e. clusters of genes or operons ), full chromosomes or entire genomes , of any organism. (
  • Information obtained using sequencing allows researchers to identify changes in genes, associations with diseases and phenotypes, and identify potential drug targets. (
  • Medical technicians may sequence genes (or, theoretically, full genomes) from patients to determine if there is risk of genetic diseases. (
  • The ArcherDx companion diagnostic assay is a sequencing-based test analyzing more than 50 genes intended for detection of somatic alterations in circulating tumor DNA (ctDNA) present in plasma and in RNA or DNA derived from formalin fixed paraffin embedded (FFPE) cancer tissue. (
  • Taken together, those features allowed us to develop a high-throughput low-cost HLA sequencing workflow that would render typing of the transplantation-relevant genes and exons affordable for donor centers. (
  • For a fee of £700-£1,800, the companies claim to be able to provide information about the person's predisposition to disease, medicines they might be sensitive to, and whether they carry any disease-causing genes that could be passed onto their children. (
  • Putting our platforms together, we believe we will be able to expedite the identification and genetic engineering of TCR genes to create personalized T cell therapies for cancer, bringing new hope to address the current limitations of treatments available today. (
  • These TCR genes are engineered into patient-derived T cells directing them towards neo-antigens in tumor cells, thereby providing a fully personalized engineered T cell therapy for cancer. (
  • With it, researchers have modified genes to help blind people see, cure sickle cell disease in some patients and speed up the development of numerous new drug treatments. (
  • Our new in situ sequencing (ISS) technology makes it possible to sequence barcoded RNA directly inside tissue sections, fulfilling the need to simultaneously analyze the expression of hundreds of genes within every cell of a tissue section with sub-cellular resolution and high throughput (~10cm2 tissue/week), while preserving the tissue morphology. (
  • The assembly of the final artificial bacterial chromosome with the imbedded natural genes and the "watermark" sequences was confirmed using several molecular techniques, and the chromosome ultimately inserted into a bacterial cell of a closely related yet distinctive species ( Mycoplasma capricolum ) where the original DNA had been expunged. (
  • Thus, the researchers were left with a "synthetic" cell from M. capricolum that was now producing proteins from a "synthetic" DNA molecule coding for genes originally from M. mycoides . (
  • Furthermore, the experiment demonstrated that there is a minimal set of genes that can be used to run a basic bacterial cell. (
  • So far, only few genes have been unambiguously validated as direct EWS-FLI1 targets in the context of Ewing cells. (
  • CRAIG VENTER has information that a lot of people want - details of DNA sequences that could identify 35 000 human genes, more than a third of the total thought to exist. (
  • So far, researchers have focused mainly on sequencing the genes, which define the structure of proteins in the human body, and locating their positions on the chromosomes. (
  • But spotting these essential sequences amid the long strands of DNA that do not code for proteins is a difficult task, and researchers have fully sequenced only a fraction of all human genes. (
  • But sequencing whole genes would have taken years. (
  • Researchers map learning-induced chromatin alterations in mouse brain cells, and find that many affect autism-associated genes. (
  • It is thought that the histones stay with the same sequences of DNA after cell replication, so the modifications of the histones and DNA allow the same sets of genes to be turned on and off in the daughter cells as in the parent cell. (
  • The companies are promoting BD's powerful BD FACS ™ single-cell sorting instrumentation and software with Cellular Research's Precise ™ assays based on Molecular Indexing ™ technology to offer customers an integrated workflow for measuring both nucleic acid and protein expression in cellular subtypes. (
  • Knowledge/expertise in molecular and cell biology skills such as RNA isolation, cloning, RT-PCR, sequencing, data analysis and cell-based assays. (
  • Founded in San Francisco in 2005, Fluxion Biosciences manufactures analytical instruments for functional cell-based assays and has won two R&D 100 Awards in the last three years. (
  • Because HRS cells are not present in high numbers in CHL tumors or the blood, attempts to develop clinical assays to evaluate minimal residual disease (MRD) in CHL have not resulted in any tests that could be adopted into regular clinical practice. (
  • Performed MTT cell viability assays: VM. (
  • LIFE ) announced today that it has signed a collaborative research agreement and related license with Harvard University under which it has acquired exclusive rights to develop a panel of characterization assays designed to rapidly evaluate human pluripotent stem (hPS) cells for their utility in a variety of discovery and translational research applications. (
  • The panel of assays was developed by Alex Meissner , Ph.D., Associate Professor, Department of Stem Cell and Regenerative Biology, Harvard University, and is being further studied and validated in collaboration with Life Technologies. (
  • Minimal residual disease (MRD) assays - capable of finding cancerous cells that otherwise go undetected - already offer prognostic information for childhood acute lymphoblastic leukemia and acute myeloid leukemia. (
  • Our intention is to complete clinical trials and pursue 510(k) clearance of the 3500 Dx system and SeCore kits to demonstrate the utility of capillary electrophoresis and sequence-based assays as powerful tools in the molecular diagnostic laboratory," Laird continued. (
  • In contrast to an enzyme-linked immunosorbent assay (ELISA) for quantifying MAbs, mass spectrometry-based assays do not rely on MAb-specific reagents such as recombinant antigens and/or anti-idiotypic antibodies, and time for development is usually shorter. (
  • To track potency, therefore, companies regularly perform quality control assays. (
  • HemaCare provides cell counts and purity data before precursor material is sent out to customers (see Figure 1), and pharmaceutical companies on the receiving end will often repeat quality control assays once they arrive in house, and at crucial points along the manufacturing workflow. (
  • the San Diego-based company whose products sequence 90 percent of all genetic data. (
  • That makes it possible to sequence very small amounts of genetic material-like, say, a single cell's worth. (
  • Not like one full of paper books-this one is built on a small glass plate with depressions, called wells, that separate genetic material from different cells. (
  • The company can use genetic sequence of the virus that China released to get started on its vaccine, and doesn't need a sample of the virus itself, said Tal Zaks, the company's chief medical officer. (
  • The Moderna vaccine works by converting genetic sequences of the virus into RNA which when injected into the body, causes cells to make a protein that stimulates immunity to the disease. (
  • This report reviews the main sequencing platforms, and explains why genetic variation is important for many markets, including clinical testing. (
  • Presentations at the conference highlighted the power of PacBio's long and accurate sequencing reads to resolve difficult regions and access novel genetic variation. (
  • We believe that this combination of unique capabilities, Molecular Indexing of genetic markers from Cellular Research and single-cell sorting using protein markers from BD, will significantly increase the ability to identify and analyze cell populations for basic and clinical research," said Stephen Fodor, Ph.D., Chief Executive Officer of Cellular Research. (
  • The DNA of cancer cells is littered with mutations - tiny genetic missteps that can make cells grow out of control or become resistant to certain medicines. (
  • We are utilizing the precision provided by nucleic acid-based therapeutics to target the sequences responsible within the genetic code. (
  • The January/February issue of RAFI CommuniquÈ examined the patenting of human genetic material, and the international controversy over the US government's patent applications on the human cell lines of indigenous peoples. (
  • Celera's genetic maps would eventually be available on the Internet, and the company will claim royalties from any commercial pharmaceutical application of its discoveries. (
  • The panel, which will be offered on the company's market-leading semiconductor sequencing and PCR-based genetic analysis platforms, will help overcome major hurdles that impede stem cell technology from moving into the clinic. (
  • Life Technologies Corporation (NASDAQ: LIFE) is a global biotechnology company that is committed to providing the most innovative products and services to leading customers in the fields of scientific research, genetic analysis and applied sciences. (
  • For the past two years, the company has been collecting samples from markets in the United States and analyzing them to expand its genetic collection. (
  • Now the company claims it has the world's largest database of food genetic markers. (
  • That's why Clear Labs decided it needed to build its own database-repeatedly sequencing the same species that show up in food products until it had a consistent description of the genetic markers within. (
  • The company won't say exactly how many food genetic markers it has, but it's confident it has more than any of the main databases, and it will continue to expand now that Clear Labs has launched its first product, Clear View, in private beta. (
  • Many cells repair the DNA break incorrectly or introduce mistakes in the process, and other cells may not even express the necessary repair machinery to insert new genetic payloads. (
  • This is a form of genetic testing , though some genetic tests may not involve DNA sequencing. (
  • ArcherDX is advancing molecular pathology with a robust technology platform for genetic mutation detection by next-generation sequencing. (
  • A genetic test is the analysis of human deoxyribonucleic acid (DNA), ribonucleic acid (RNA), chromosomes, and proteins to detect heritable disease-related genotypes, mutations, phenotypes, or karyotypes (standard pictures of the chromosomes in a cell) for the purposes of diagnosis, treatment, and other clinical decision making. (
  • Most genetic testing is performed by drawing a blood sample and extracting DNA from white blood cells. (
  • Genetic tests may detect mutations at the chromosomal level, such as additional, absent, or rearranged chromosomal material, or even subtler abnormalities such as a substitution in one of the bases that make up the DNA. (
  • Today, precision cancer treatments are hamstrung by their ability to understand the genetic diversity and rare cells in tumors that can drive disease progression. (
  • The system targets the genetic variants that are important, providing the highest-resolution picture of disease-relevant cell populations with single-base pair resolution orders of magnitude better than the megabase resolution of other single-cell DNA approaches. (
  • How this can help you to discover, map and validate new genetic markers and cell types. (
  • If the telomeres get too short, it is a signal for the cell that its genetic material is compromised and the cell stops dividing. (
  • Using 26 pediatric ependymoma patient samples, researchers sequenced 18,500 single cells to classify and analyze discrete neoplastic subpopulations. (
  • Researchers developed a highly multiplexed screen to quantify the co-expression of 351 molecules on the surfaces of millions of human B cells. (
  • To minimize the chances that HeLa or other cell lines will cause cross-contamination problems-and potentially give rise to spurious results-researchers are encouraged to follow "best practices" recommendations, such as those issued by the Global Biological Standards Institute. (
  • Today's announcement represents an exciting step forward for researchers engaged in single-cell genomic analysis" said Claude Dartiguelongue, Worldwide President, BD Biosciences. (
  • SEATTLE , April 28, 2015 /PRNewswire/ -- Adaptive Biotechnologies today announced the recent publication of a study conducted with researchers at The University of Texas MD Anderson Cancer Center showing that the company's next-generation sequencing-based approach to identification and quantification of populations of B cells can detect tumor-specific DNA sequences in the blood of people with classical Hodgkin's lymphoma (CHL). (
  • We are excited to offer this technology to the wider community of clinical researchers, biotechnology and pharma companies. (
  • Researchers may order cells from the tumor catalog, acquire microarrays of sections of formalin-fixed, paraffin-embedded clinical tumor samples, or obtain samples from various NIH-sponsored repositories of chemical and biological substances. (
  • Standardizing the way researchers characterize iPS cells will allow th. (
  • Standardizing the way researchers characterize iPS cells will allow them to quickly identify the most promising cell lines and avoid wasting time and resources on cells that do not possesses the appropriate characteristics. (
  • Researchers looked at blood samples from 43 leukemia patients who got high-dose chemo, and found that a new DNA sequencing-based technology was able to spot trace amounts of cancer in 22 patients. (
  • Researchers sought to get this answer through a new technology based on advances in DNA sequencing. (
  • The company uses San Diego-based Illumina's high-speed HiSeq DNA sequencing instruments, and it has custom chemical reagents and software that enable researchers to look specifically at DNA sequences of these variable T and B cells, Robins says. (
  • The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography . (
  • Sequencing enables researchers to determine which types of microbes may be present in a microbiome , for example. (
  • Dr. Jason Bielas, who is collaborating with the researchers in the first phase of the grant competition, says the goal of the awarded pilot project is to use single-cell RNA sequencing to develop better immunotherapy-based cancer treatments while addressing fundamental questions in biology. (
  • The researchers, each of whom received $25,000 in exploratory funding, will be invited to compete for a $100,000 pilot award based on their initial findings and plans for future studies. (
  • Single-cell RNA sequencing allows researchers to distinguish between different types of cells in a complex mixture and analyze data patterns among thousands of individual cells - all within a single experiment. (
  • If selected for the pilot phase, the researchers will expand the study to examine the mechanisms of T-cell treatment response in nine MCC patients. (
  • For the first time, researchers have successfully created airway basal stem cells in vitro from induced pluripotent stem cells by reprogramming blood cells taken from patients. (
  • A team of researchers from Cologne and Helsinki has discovered a mechanism that prevents hair loss: hair follicle stem cells, essential for hair to regrow, can prolong their life by switching their metabolic state in response to low oxygen concentration in the tissue. (
  • Researchers at Columbia University Medical Center have developed three-dimensional lung bud "organoids" from human pluripotent stem cells. (
  • Founded in 2007 by researchers from the University of New Mexico, nanoMR is an early-stage life sciences company developing novel diagnostic systems for the rapid isolation of rare cell types, including bacterial and fungal pathogens, directly from blood and other matrices. (
  • Single-cell approaches enable researchers to rapidly and comprehensively analyze cells for type and function, which cannot be done for intact tissues. (
  • Researchers have created microfluidic devices for (a) digestion, (b) disaggregation, and (c) filtration of tissue samples to generate single cells. (
  • After human cells are modified, the researchers can run them through a battery of tests to ensure that they meet stringent requirements before administering them to patients. (
  • To address this, Mission Bio has created Tapestri, a single-cell genomic technology that reveals the molecular profile of each individual cell in an actionable timeframe, empowering researchers and clinicians to create precise therapies and detect rare diseased cells that may persist after treatment, causing relapse. (
  • The Tapestri Platform is now compatible with Illumina NovaSeq and HiSeq sequencers, reducing sequencing costs from roughly $1,100 to $200 per run, enabling researchers to do more with their budgets," said Silver. (
  • Mission Bio also recently announced Tapestri Custom Panel Designer, a web-based application enabling researchers to easily design single-cell DNA panels to target their specific regions of interest. (
  • Just by observing the sequence of those bases, stored in each and every human body cell, researchers can create a portrait of the person they come from, from their hair colour to the likelihood they'll get breast cancer to their sensitivity to bitter tastes. (
  • Scientists have been excited about CRISPR since 1987, when a team of Japanese researchers accidentally cloned clusters of cells at Osaka University. (
  • Researchers grew the cells through a number of cell divisions, which eventually diluted out, degraded, and replaced the original M. capricolum proteins. (
  • Dr. Harding said that Life Tech "certainly expects" to see useful enzymes and research chemicals discovered during the research, but the most commercially important discovery will be the improved instruments for DNA sequencing -- if the researchers can perfect them, which isn't a cinch. (
  • Danaher Corporation is the largest player in the Cell analysis market with a share of XX%, followed by Thermo Fisher Scientific and Becton Dickinson accounting for XX% and XX% shares respectively in 2013. (
  • The NIH is working with a U.S. company Moderna Therapeutics to get a separate promising vaccine prospect into early testing within three months, NIAID director Anthony Fauci said this week. (
  • Apart from the growing applications of oligos, increasing R&D expenditure in pharmaceutical and biotechnology companies, the evolving significance of RNA-interference oligos in therapeutics and diagnostics, increasing venture capitalist and governmental funding and support, and rapid technological innovations are other factors driving this market's growth. (
  • Novartis recently entered a collaboration with Intellia Therapeutics, a startup biotechnology company, to sharpen this powerful tool for patients. (
  • SOUTH SAN FRANCISCO, Calif. & AMSTERDAM & NEW YORK--( BUSINESS WIRE )-- Twist Bioscience Corporation (NASDAQ: TWST), a company enabling customers to succeed through its offering of high-quality synthetic DNA using its silicon platform, and Neogene Therapeutics, Inc. , a biotechnology company pioneering a new class of fully personalized T cell therapies to treat cancer, today announced a broad strategic partnership. (
  • Neogene Therapeutics is a pre-clinical stage biotechnology company pioneering development of next-generation, fully personalized engineered T cells therapies for a broad spectrum of cancers. (
  • Argos Therapeutics is one of several pharmaceutical companies that helped lay the groundwork into developing such precision therapeutics, which are based on the guided manipulation of living cells. (
  • The clinical success of cell-based therapeutics is inextricably linked to the purity and potency of precursor raw materials. (
  • In regards to our current base business, due to a delay of the launch of a clinical test by a large reference lab customer, as well as some ongoing commercial challenges in Europe due to overall conditions in this market, we are revising our full-year 2015 revenue guidance to between $7.5 million and $7.8 million from $8.0 million to $8.5 million," continued Dr. Carlson. (
  • The company supports the heroes on the frontlines of health care by developing innovative technology, services and solutions that help advance both clinical therapy for patients and clinical process for health care providers. (
  • Adaptive Biotechnologies' clonoSEQ process enables physicians to utilize sequencing-based minimal residual disease (MRD) detection as an aid to clinical decision making for patients with lymphoid cancers (blood cancers). (
  • The outfit is one of several companies striving to create fast, accurate, and affordable sequencing methods, which could ultimately have a broad impact on both cancer research and clinical practice. (
  • The market evaluation in the study pertains to existing processes and/or devices used in the mobile application of DNA sequencing, and which have been developed and approved for human clinical use. (
  • Secondary outcomes include, besides clinical endpoints, amyloid PET scanning with Piramar Imaging's tracer Neuroceq (formerly Bayer's florbetaben), as well as biomarker measures such as MRI volumetry, tau, phospho-tau and Aβ levels in CSF, and measures of T-cell activation. (
  • The widespread use of flow cytometry, polymerase chain reaction and next-generation sequencing has allowed clinicians to more accurately determine the depth of clinical complete remission. (
  • When PCR technology came into clinic, oncologists began to realize that young adults with ALL who for years were in clinical complete remission were found to have minute numbers of demonstrable leukemic cells. (
  • CARLSBAD, Calif. , March 21, 2011 /PRNewswire/ -- Life Technologies Corporation today announced that it is the first life science tools provider entering clinical trials to prove the safety and effectiveness of their DNA sequence-based typing (SBT) platform for analysis of the Human Leukocyte Antigen (HLA) system. (
  • While some companies wait until clinical trials are finished with dosing and collecting data to begin releasing information, those that release interim data can give investors a better handle on how t. (
  • The PointMan system preferentially amplifies variant sequences of interest while suppressing amplification of wild type DNA, giving it the potential to identify all mutations associated with the clinical utility of targeted cancer therapies. (
  • Ortho-Clinical Diagnostics has entered an agreement with commercial-stage diagnostics company Quotient Limited to distribute and sell Quotient's transfusion diagnostics platform MosaiQ. (
  • The new technology has potential to meet this demand through its use in Next Generation Sequencing (NGS)-based clinical diagnostics. (
  • A new technology that could perform the entire process in a rapid, gentle, thorough, and automated manner would dramatically advance single-cell diagnostics and boost their clinical potential, thus paving the way for powerful new personalized treatments. (
  • The company said 2018 revenues grew by 94 percent over 2017 revenues, driven by its clinical tests as well as partnerships with pharmaceutical companies. (
  • Twist will receive technology access fees as well as milestones and royalties based on key preclinical, clinical and commercial milestones for any antibodies and T cell receptors resulting from the collaboration. (
  • A background in physiotherapy helps the Oxford-based researcher mix basic science with clinical work to better understand pain stemming from nerve compression. (
  • The present study aimed to discuss the molecular mechanism of microRNA-93 as it regulates the proliferation of cervical cancer cells, and further provide theoretical basis for the clinical treatment and prevention of cervical cancer. (
  • Initially focused on preventing infectious diseases, the company has demonstrated the potential of its versatile platform through the rapid progression into Phase 2 clinical development for a vaccine against Chikungunya, a debilitating disease with global outbreak potential. (
  • Argos has operated for years in the personalized immunotherapy space, using cellular material collected from both patients and healthy donors to carry out clinical trials based on using dendritic cells to trigger a targeted, patient-specific immune response. (
  • A compatible fluorescence-based assay for antibody titer also has been validated. (
  • The line is exposed to a CRISPR library and sorted using the fluorescence-based assay for those cells with higher MAb expression. (
  • BOULDER, Colo. , Jan. 8, 2019 /PRNewswire/ -- ArcherDX, Inc., a molecular technology company dedicated to developing breakthrough solutions that advance the application of personalized genomic medicine, today announced that the U.S. Food and Drug Administration (FDA) granted a Breakthrough Device designation (formerly Expedited Access Pathway program) for ArcherDx's companion diagnostic assay application. (
  • Results of the ArcherDX companion diagnostic assay will be used to identify patients diagnosed with advanced non-small cell lung cancer for whom treatment with a specific targeted therapy is being considered. (
  • This apart, the company has entered into an agreement with Eli Lilly and Company ( LLY - Free Report ) for developing a companion diagnostic that will use the FDA-approved, next-generation sequencing-based Oncomine Dx Target Test to identify certain non-small cell lung cancer (NSCLC) and thyroid cancer patients who may be treated with Lilly's investigational therapy, LOXO-292. (
  • A Comprehensive High Throughput Single Cell Analysis Workflow Combining Proteomic and Genomic Information: Flow sorting, single cell tagging, molecular barcoding, library preparation, and sequencing analysis. (
  • This creates rich data sets to characterize cell populations based on surface markers in parallel with genomic processes inside the cell. (
  • See RAFI's survey of genomic companies (page 4-6). (
  • What is a "genomic" company? (
  • For the most part, new genomic companies are not concerned with the location and analysis of all human DNA. (
  • In recent months, transnational pharmaceutical companies and venture capitalists have made major investments in new genomic companies. (
  • Adaptive's bet is that its technology will be more automated, and easier to standardize, largely because it's one of only a handful of organizations developing this sort of T and B cell genomic technology (San Francisco-based Sequenta is another). (
  • The Newborn Sequencing in Genomic Medicine and Public Health (NSIGHT) study involves four hospitals and is running for five years from 2013. (
  • Precision medicine tailors treatments, practices and products to the individual patient based on their genomic sequence. (
  • HGS sees this seemingly modest demand as a way to pay back its investors, such as the pharmaceuticals company SmithKline Beecham, which has poured $100 million into HGS and its non-profit arm, The Institute for Genomic Research (TIGR). (
  • The NimbleGen Sequence Capture technology is a sophisticated process for the parallel enrichment of selected genomic regions from complex human genomic DNA. (
  • The markets for sequencing products and services are given for the years 2012, 2013 and 2018. (
  • The markets for NGS-based NIPT are given for the years 2017, 2018 and 2023. (
  • The UK's 100,000 Genomes Project aims to sequence 100,000 genomes from adults and children who have rare diseases and cancer by the end of 2018. (
  • The company issued shares to the public in March 2018, raising $100 million. (
  • VIENNA, Austria I October 11, 2018 I Themis announced today that it has entered into a license agreement with Max-Planck-Innovation GmbH, the technology transfer agency of the Max Planck Society in Germany, granting it exclusive worldwide license to develop, manufacture and commercialize therapies based on an oncolytic measles virus platform that was jointly developed by the Eberhard-Karls-University Tübingen and the Max Planck Institute for Biochemistry. (
  • The combined workflow enables cells to be individually sorted into 96- or 384- well Precise encoding plates using the BD FACS ™ instruments and software. (
  • Our investment in this important work developed at Harvard University supports our sustained commitment to provide our customers with the most innovative tools for the iPS cell workflow. (
  • By exponentially amplifying cell-free DNA derived from apoptosis, the cell-death mechanism, these TruePrime kits provide users with excellent sensitivity, error-free amplification in high yields, and a streamlined workflow for reduced hands-on time. (
  • of California, Irvine, has developed a novel microscale fluidic device that can perform the entire tissue dissociation workflow while gently and efficiently producing single cells. (
  • However, all three devices are currently being combined into an integrated platform, the first of its kind, that can perform the entire tissue-to-cell workflow. (
  • The workflow presented proved to be a cost-efficient alternative to Sanger sequencing for high-throughput HLA typing. (
  • QIAGEN's liquid biopsy solutions empower you to sensitively, specifically and rapidly reveal meaningful insights from your samples with an efficient, streamlined and complete workflow for analyzing circulating cell-free DNA, exosomes and CTCs for PCR and NGS applications, giving you the first step towards uncovering valuable biomarkers in your samples. (
  • MT. LAUREL, N.J., Sept. 23 /-- The release of a new, simple-to-use diagnostic tool for federal government agencies and government contractors to perform self-evaluation based on the GAO Cost Assessment Guide requirements was announced today by PRICE Systems (, the world leader. (
  • A look at substantial future growth opportunities for industry participants including consumer and diagnostic sequencing services, bioinformatics, sequence enrichment, benchtop NGS sequencers and consumables, and emerging sequencing instruments. (
  • In the first step, the clonoSEQ ID test, cancer cell DNA sequences are identified in a diagnostic sample. (
  • Oligonucleotides synthesis market is segmented on the basis of end users as pharmaceutical and biotechnology companies, academic research institutes and diagnostic laboratories. (
  • The booming healthcare technology market with demand for innovative technology for early and accurate diagnosis of life threatening diseases, increasing government and corporate funding and investment in cell based research, higher demand from end users such as diagnostic laboratories for cell analysis products are some of the factors driving the cell analysis market growth. (
  • The company manufactures both molecular diagnostic and research use only products. (
  • Both large and small biopharmaceutical companies are now actively developing drug-diagnostic combination products. (
  • There are ample opportunities for growth in this niche market for both pharmaceutical and diagnostic companies. (
  • and the prenatal diagnostics (including market shares for the leading NGS companies). (
  • BD is one of the largest global medical technology companies in the world and is advancing the world of health by improving medical discovery, diagnostics and the delivery of care. (
  • EKF Molecular Diagnostics has entered a collaboration with the specialist medtech company Angle to develop a liquid biopsy combining Angle's Parsortix circulating tumor cell (CTC) harvesting platform with EKF's PointMan DNA enrichment technology. (
  • Microsoft has thrown its weight behind Adaptive Biotechnologies' attempt to develop blood-based diagnostics. (
  • Together, the partners think they can develop blood-based diagnostics. (
  • This biological insight has combined with technological advancements to help usher in the era of single-cell diagnostics for tissue-based diseases. (
  • The concept of companion diagnostics holds huge potential for pharma companies as well as medical community. (
  • INanoBio is a startup based out of Tempe, AZ and Menlo Park, CA. The company's mission is to develop high-accuracy early stage disease diagnostics using transformative nano-biotechnology platforms and machine learning. (
  • Roche is also the world leader in in-vitro diagnostics, tissue-based cancer diagnostics and a pioneer in diabetes management. (
  • This new TruePrime apoptotic cell free DNA amplification kit launch comes at a very important period in the growing liquid biopsy market, as applications for the liquid biopsy market will become an important growth driver for Sygnis. (
  • Seattle-based Adaptive is approaching from a different angle the question of how to diagnose cancers and other diseases from blood samples, hoping to stand out from other companies pursuing the liquid biopsy dream. (
  • Drugs for an underserved liver disease class, continued liquid biopsy development, generic biologics, smokeless tobacco abuse, a new chip to improve reads and run lengths for NGS and many company updates were on Kalorama's radar at the 37th J.P. Morgan Healthcare Conference. (
  • Guardant RUO test: The test, a targeted sequencing-based test of cell-free DNA, builds on its Guardant360 liquid biopsy for tumor mutational profiling, by adding an epigenomic component. (
  • The products combine next-generation sequencing and analysis with the company's NGSengine software to provide high-resolution HLA genotyping information. (
  • Sensitive molecular technologies, such as the next-generation sequencing utilized by Adaptive Biotechnologies' clonoSEQ MRD test, are needed for reliable detection of very low levels of MRD. (
  • We have developed a high-sensitivity, next-generation sequencing-based system for the the detection of cancer mutations in miniscule concentrations of circulating cell-free DNA (cfDNA) in blood. (
  • In addition, rising field of next-generation sequencing to increase demand for next generation sequencing-grade oligos would develop the opportunity for the global oligonucleotide synthesis market. (
  • Moreover, emergence of next-generation sequencing (NGS) has helped the market gain tremendous traction over the past few years. (
  • This report reviews the main sequencing technologies and provides detailed discussion on next generation sequencing and its applications in different fields. (
  • Unlike other companies in this space, Mission Bio's technology can analyze tens of thousands of individual cells in parallel, which, according to Silver, means that it's 50 times more sensitive than traditional next-generation sequencing techniques. (
  • The efficiencies of parallel enrichment are an ideal complement for cost-effective, high throughput next-generation sequencing. (
  • They study the stem cells that form blood-the bone marrow-derived cells that help cancer patients recover after chemotherapy destroys their immune systems. (
  • Sinha wanted to find a true blood stem cell: one that hadn't already started turning into a red blood cell, or a platelet, or an immune cell. (
  • The immune cell sequencing company beat Wall Street estimates on the top line, but missed on the bottom line for both Q4 and full-year 2020. (
  • Our proprietary single cell platform is our core technology, which enables deep survey of immune repertoire to identify rare antibodies, it also provides a revolutionary approach to interrogate TCR and its interaction with cognate antigens. (
  • Faced with a new, deadly and fast-spreading disease, drug designers started work without knowing whether antibodies' ability to activate a variety of immune cells would aid or hinder efforts to control the disease. (
  • It attaches to receptors on immune cells, inducing them to kill infected cells and release molecules that sculpt the immune response. (
  • In a process known as antibody-dependent enhancement, interactions between the long arm of antibodies and immune cells can worsen some viral infections, notably infections with the tropical dengue virus. (
  • To avoid the danger of antibody-dependent enhancement, some companies developing antibody-based COVID-19 drugs changed the sequence in the long arm of the antibodies to prevent it from interacting with immune cells. (
  • They eliminated the antibody's effector functions by mutating its long arm so that it could not stimulate immune cells. (
  • Using sc-RNA-seq of the patient's tumor samples collected over time, Chapuis' group will simultaneously investigate the immune responses from the perspective of the patient's tumor cells, connective-tissue cells and immune cells, and determine how these cell populations changed in response to various treatment regimens. (
  • Reflecting its background in the profiling of T-cell and B-cell receptors, Adaptive is looking to changes in the immune system for clues about the disease state of patients. (
  • Adaptive is bringing its immune sequencing technologies to the collaboration. (
  • But advances in sequencing and Adaptive's work to understand the immune system mean the company at least has a roadmap toward the objective. (
  • Adaptive is building the TCR antigen map, a relational database, upon its immune sequencing work. (
  • The use of FCS to supplement cell culture medium for AAV particle production is problematic because even smallest amounts of animal antigens in the administered drug could lead to a strong immune response in patients. (
  • This is a realistic goal because our core technology can be engineered into a closed system and does not perturb immune cells. (
  • Thus far, it appears that Indee Labs' technology can rapidly process clinically- and commercially-relevant samples with no observed immune cell perturbation, likely due to the gentle nature of fluid dynamics coupled with rapid processing times. (
  • Immune-like cells in the central nervous system are now recognized as key participants in the creation and maintenance of persistent pain. (
  • Through advanced understanding of immune system mechanisms, the company has built a sophisticated and versatile technology platform for the discovery, development and production of vaccines as well as other immune system activation approaches. (
  • 2] In cell therapy, potency is often directly correlated with the number of healthy immune or stem cells in your finished product. (
  • At Argos, the number of healthy immune cells present in the precursor material was particularly important, because they were dealing with monocytes, a cell type that doesn't replicate. (
  • and DNA base modification identification to help characterize epigenetic regulation and DNA damage. (
  • While the N-terminal portion of both the light and heavy chains contains the variable-region sequences that give the Ig its specificity for binding of antigens (Fab), the C-terminal portion of both chains (Fc) contains the constant-region sequences that characterize the Ig by isotype and class ( 1 , 2 ). (
  • Based on this information, it is likely that CCDC130 plays a similar role in the human spliceosome, but due to the higher complexity of the human spliceosome, this protein may perform other functions or a completely different function. (
  • There were several predicted promoters found for CCDC130 using ElDorado from Genomatix, but the promoter that corresponds the closest to the protein sequence is 760 bases and spans from 13858094-13858853 on chromosome 19. (
  • The first is the COG5134 domain which is found to be conserved in cucumbers and likely plays a role in the function of the protein because it is always the most highly conserved region in any multiple sequence alignment. (
  • Highly motivated, willing to work in a startup company, eager to learn/develop protein chemistry and molecular cell biology techniques. (
  • Ltd. has entered into an exclusive worldwide licensing agreement with the National University of Singapore (NUS) for Enhanced Single Cell Analysis with Protein Expression (ESCAPE TM ) RNA sequencing technology, an innovative multifaceted single-cell sequencing platform for simultaneous protein and RNA measurements. (
  • The technology enables single cell RNA sequencing platforms to efficiently detect and quantify protein expression. (
  • Retinal pigment epithelium (RPE) cells secrete a unique 50 kD protein. (
  • This protein, pigment epithelium-derived factor (PEDF), contains trophic activity in both the induction of neuronal cell differen. (
  • Pigment epithelium-derived factor (PEDF), a unique 50 kD protein secreted by retinal pigment epithelium (RPE) cells, has trophic activity. (
  • Based on sequence motif and structural elements of the CCRs we have designed an artificial protein that is capable of inhibiting the 20S proteasome. (
  • A cell extract containing the recombinant protein ist then applied to a collumn containing immobilized Ni2+-Ions. (
  • The CRISPR/Cas system uses a "guide" RNA to bring the DNA-cutting Cas protein to a specific DNA sequence that contains a disease-causing mutation. (
  • Once the Cas protein cuts the DNA at that target site, the system replaces the faulty DNA sequence with a healthy version. (
  • Blom N, Gammeltoft S, Brunak S (1999) Sequence and structure-based prediction of eukaryotic protein phosphorylation sites. (
  • Plewczynski D, Basu S, Saha I (2012) AMS 4.0: consensus prediction of post-translational modifications in protein sequences. (
  • Guillon N, Tirode F, Boeva V, Zynovyev A, Barillot E, Delattre O (2009) The Oncogenic EWS-FLI1 Protein Binds In Vivo GGAA Microsatellite Sequences with Potential Transcriptional Activation Function. (
  • LIFE ), a leading maker of DNA-sequencing equipment and laboratory supplies used in biopharmaceutical research and manufacturing, said it has acquired BAC, a privately held company in the Netherlands that develops and makes protein purification products. (
  • Nonsense mutations are significant, because they are point mutations in a sequence of DNA that cause a premature stop codon, or a nonsense codon in the transcribed mRNA, resulting in a truncated, incomplete, and usually nonfunctional protein. (
  • The results showed that the expression of nAG protein in primary human fibroblast cells suppresses the expression of collagen I and III, with or without TGF- β 1 stimulation. (
  • nAG protein expression by schwann cells of regenerating axons peaks at 5-7 days postamputation. (
  • The nAG protein is released by schwann cells. (
  • A universal blood stem cell could reveal the path to all its progeny, helping scientists custom-make any blood cell a patient needed. (
  • Scientists who manage the sequencing core facilities for the University of California system estimate that the system, introduced in January 2015, handles 90 percent of its sequencing requests. (
  • Based on past experience, though, the chances of all that falling into place fast enough to turn the tide aren't great, many scientists say. (
  • But there are tremendous opportunities, both scientifically and financially-huge amounts of funds potentially saved-if the majority of scientists authenticated their cell-lines. (
  • Pacific Biosciences of California, Inc. (Nasdaq:PACB) offers the PacBio ® RS II Sequencing System to help scientists resolve genetically complex problems. (
  • Because each sequencing reaction takes place in an isolated reactor, scientists can detect rare mutations, which might occur in only a few wells. (
  • We are seeking a passionate and creative, Associate Scientist to join our Cell Biology team and work with an enthusiastic team of highly skilled scientists in a dynamic, high growth company. (
  • The findings, available online in the journal Cell, could help scientists improve the next generation of antibody-based COVID-19 drugs. (
  • Scientists at the Fred Hutchinson Cancer Research Center, the University of Washington and Seattle-based Adaptive Biotechnologies are reporting they've found what could become a more clear and consistent way to spot "minimal residual disease" that doesn't show up on imaging scans, but can be deadly. (
  • Flow cytometry, a technique that scientists have used for years, looks for certain biomarkers that can be found on the surface of cancer cells. (
  • Stem cell-filled implants helped repair spinal cord damage in animals, according to a study led by UC San Diego scientists . (
  • Five Fred Hutchinson Cancer Research Center scientists have received the first phase of funding in a grant competition sponsored by the Immunotherapy Integrated Research Center and funded by the Bezos family Immunotherapy Initiative to conduct projects using single-cell RNA sequencing, also known as scRNA-seq. (
  • Much like recording the cell phone traffic of each individual in an entire city would show similarities and differences between various groups of people (teenagers, people conducting business, etc.) the platform, for example, may help scientists determine how different cell types interact in the complex environment of a tumor. (
  • For example, this technology could provide valuable insight into cancerous and non-cancerous cells that could help scientists predict disease progression and select the most effective therapies to bring about a cure for cancer. (
  • The scientists used the bioinformatics tool, Kontaminant in their research, which was originally developed for screening DNA for contamination from other species and then adapted this tool to analyse metagenomic samples and identify species as they are being sequenced on the MinION. (
  • Additionally, the scientists were challenged with the complex task of assembling the DNA fragments into a much larger one million base-pair (Mbp) synthetic chromosome. (
  • If you're going from sequencing 10,000 base pairs a day to 1,000 base pairs a second, you can see the tremendous impact it will have," especially for scientists who have 3 billion base pairs to sequence and then decipher, Dr. Harding said. (
  • Our interdisciplinary team provides consulting and support for scientists in evaluating inventions, applying for patents, and founding companies. (
  • In their latest paper, published in the journal Oncogene, the scientists used live imaging and electron microscopy to study the cellular activities associated with successful metastasis, including the expression of a group of proteins called cadherins, which help cells bind together. (
  • Among other strategic developments, we make a note of Thermo Fisher acquiring the Advanced Bioprocessing business from Becton, Dickinson and Company ( BDX - Free Report ) , which is expected to significantly widen the company's Life Sciences Solutions line of offerings. (
  • The company's first product addresses the blood culture market, demonstrating identification of blood-borne infections in less than two hours, compared with days required for conventional blood-culture-based systems. (
  • The Company's engineered T cells target tumor neo-antigens, mutated proteins found in cancer cells due to cancer-associated DNA mutations, that render tumor cells vulnerable to detection by T cells. (
  • The Los Alamos contract is an attempt by Life Tech to hit a home run by developing a small handful of blockbuster instrumentation products, an uncharacteristic move for a company usually content to rely on a broad portfolio of products, few of which are major individual contributors to the company's sales. (
  • While Hotez and Bottazzi try to wrangle finances for their work, a London-based group called the Coalition for Epidemic Preparedness Innovations, or CEPI, which was created in 2017, has already committed $12.5 million to two U.S.-based startups and an Australian university with promising coronavirus vaccine platforms. (
  • nanoMR's products use an immunomagnetic capture process that can deliver either viable cells for phenotypic analysis, or extracted DNA for molecular analysis by different DNA-based platforms, including PCR, hybridization and DNA sequencing. (
  • Having developed their experimental and data methods, they then tested real environmental samples sequencing them on the MinION and Illumina platforms for comparison. (
  • Ultra-fast giga-hertz sampling rate of FENT transistors will enable epigenetic and DNA sequencing at a rate of up to million bases per second per pore, which is over a thousand times faster than other state of the art nanopore sequencing platforms. (
  • Sino-American Silicon Products (SAS) is preparing for production of N-type TOPCon (tunnel oxide passivated contact) solar cells, with product certification to begin in mid-2019, according to company president Doris Hsu. (
  • Global Biotechnology Market Research Report 2019 Contains Data and the information regarding the market are taken from reliable sources such as websites, annual reports of the companies, journals, and others and were checked and validated by the industry experts. (
  • IQ4I Research & Consultancy published a new report on "Cell Analysis Global Market - Forecast To 2020" analyzes and studies the major market drivers & Opportunities, Restraints & Threats in North America, Europe, Asia-Pacific and Rest of the World. (
  • The global Cell Analysis market is expected to reach $35.4 billion by 2020. (
  • NEW YORK, May 08, 2020 (GLOBE NEWSWIRE) -- Fortress Biotech, Inc. (Nasdaq: FBIO) ("Fortress"), an innovative biopharmaceutical company, today announced that Oncogenuity, Inc. ("Oncogenuity"), a new Fortress partner company, has entered into an exclusive worldwide licensing agreement with Columbia University to develop novel oligonucleotides for the treatment of genetically driven cancers. (
  • These cells are present at levels undetectable by traditional microscopic examination (also called morphologic examination) of blood, bone marrow or a lymph node biopsy. (
  • Shotgun sequencing was initially used to sequence small genomes such as that of the cauliflower mosaic virus (CMV), which is 8kb long. (
  • Sequencing is used in molecular biology to study genomes and the proteins they encode. (
  • Which babies could have their genomes sequenced? (
  • He Jiankui says that both twins have had their genomes sequenced since birth. (
  • Sequence Capture allows enrichment of target regions in a single experiment, replacing the need to perform numerous PCR reactions. (
  • The PME free-circulating DNA Kit-IPC16 is based on a new, patent-pending technology called PME - Polymer Mediated Enrichment. (
  • New sequencing technologies that can detect rare mutations in tumors might allow personalized treatments for cancer. (
  • But sorting out the key mutations from the surrounding reams of normal DNA in tumor samples is a challenge, partly because it's difficult to isolate and sequence single molecules of DNA. (
  • But because the cancer mutations occur much less frequently than the normal sequence, the signal from the mutated sequence is likely to get lost. (
  • We found that using the 454 [Life Sciences] method, we could find mutations we would miss with conventional sequencing," says Meyerson. (
  • A lot of tumor samples from patients are limited in quantity, so it's crucial to be able to take a minute amount of tumor cells and detect mutations. (
  • The most comprehensive test for BRCA1 and BRCA2 mutations was developed by a company called Myriad Genetics and costs about $3000. (
  • But sometimes those mutations lead to T cells or B cells that divide out of control, leading to leukemia or lymphoma. (
  • The companies will leverage Neogene's proprietary expertise in targeting tumor neo-antigens, mutated proteins found in cancer cells due to cancer-associated DNA mutations, together with Twist's DNA synthesis platform and product lines to develop personalized chimeric antigen receptor (CAR) T cell therapies and T cell receptor (TCR) therapies for patients with cancer. (
  • ONCOlogues' selectivity enables Oncogenuity to target genetically driven cancers caused by mutations without impacting wild-type ("WT") DNA sequences, potentially limiting off-target toxicity. (
  • The plasma-based tests offer sensitive and cost-efficient detection of mutations in circulating tumour DNA. (
  • Changes in the RET kinase, including fusions and mutations, can cause uncontrolled cell growth leading to tumor development. (
  • Korro Bio is a privately held biotechnology company launching out of a top-tier life science venture firm headquartered in Cambridge, Massachusetts. (
  • Life Technologies Corporation is a global biotechnology company dedicated to improving the human condition. (
  • Once all the fragments were barcoded, he dumped them into a single test tube, washed away the extra barcode-containing molecules, and sequenced them. (
  • Myriad officials say they deserves the patent because they invested a great deal of money to figure out the sequence and develop "synthetic molecules" based on that sequence that can be used to test the variants in a patient. (
  • Microfluidic chips operate at physical length scales comparable to biological constituents ( e.g., cells and molecules), which is typically tens of microns. (
  • Their aim was to sequence the microscopic biological molecules in the air around us - bacteria, spores and viruses. (
  • The recipient bacterial cell ( M. capricolum ) had intact proteins and RNA molecules, which included all of the required, preformed, and functional enzymes to support self-replication once the new chromosome was inserted. (
  • Nearly 500 companies from biotech, pharma, insurance, hospital systems and more gave investor presentations to more than 9,000 attendees at the Westin St. Francis hotel this year. (
  • It includes the sequencing instrument market by platform: Sanger, NGS and 3GS and by instrument class: low throughput, high-throughput, benchtop, and handheld. (
  • However, the required sophisticated sample preparation steps and associated costs make that approach currently more attractive for patient samples than for cost-sensitive high-throughput stem cell donor registry typing. (
  • The Chinese company will use the funds to accelerate the registration and commercialization of its NGS-based products for cancer screening and early detection. (
  • With its ability to detect cancer cells at a level as low as one per one million white blood cells, the clonoSEQ MRD test is one to two orders of magnitude more sensitive than the other methods of MRD detection, such as ASO-PCR and flow cytometry. (
  • Prior to the introduction of MRD detection, patients with leukemia, myeloma and lymphoma were considered in morphological complete remission when - under microscopic analysis - they had normal neutrophil and platelet counts and less than 5% of cancerous cells present in bone marrow. (
  • To be more precisely identify isolates of V. parahaemolyticus causing AHPND, Center of Excellence for Shrimp Molecular Biology and Biotechnology ( Mahidol-BIOTEC cooperative center ) collaborated with the Aquatic Animal Health Research Center, Charoen Pokphand Company to develop a better PCR detection method. (
  • Improved immunohistochemical stains and in situ hybridization make detection of cancer cells in biopsied tissue more effective. (
  • I joined WaferGen primarily because of the potential of its SmartChip technology in single cell analysis, and during my initial tenure I have become even more convinced of the significant value it could bring to the field and its application in drug development," said Rollie Carlson, Ph.D., President and CEO of WaferGen. (
  • In addition, we continue to expect the full commercial launch of our single cell technology during the fourth quarter of 2015. (
  • Cellular Research has developed Precise, a highly multiplexed molecular and sample barcoding technology, whereby each transcript in each plated cell is uniquely barcoded during incorporation into one sequencing library. (
  • Reel Solar , a San Jose, Calif.-based developer of thin-film photovoltaic technology recently secured $15 million in investments from X/Seed Capital, and moved from a 6,000-square-foot laboratory to a 58,000-square-foot research and development facility in a northern part of the city, despite still being in stealth mode. (
  • We are pleased to have supported the academic inventors on their exciting journey from scientific discovery to spinning off a biotechnological company, for example by funding technical and business development through our internal Technology Acceleration Programme. (
  • Using a novel deep sequencing technology that can in one fell swoop de. (
  • State-of-the-art sequencing by synthesis (SBS) technology developed by Solexa Inc., in Hayward, Calif., will provide the data essential to the project. (
  • To look for those nasty T and B cells, Adaptive has built a new kind of technology platform based on DNA sequencing, which it calls ImmunoSEQ. (
  • Thus, the INTEGRATE technology, unlike other editing tools based on integrases, is the first fully programmable insertion system studied to date. (
  • It includes any method or technology that is used to determine the order of the four bases- adenine , guanine , cytosine , and thymine -in a strand of DNA. (
  • In fact, DNA sequencing has become a key technology in many areas of biology and other sciences such as medicine, forensics , or anthropology . (
  • This is a nice marriage of the technology of bioengineering and 3D printing with stem cell biology to treat a really important human disease that needs better therapy," Tuszynski said. (
  • To help clients make informed business decisions, we offer market intelligence studies ensuring relevant and fact-based research across a range of industries, from technology to chemicals, materials and energy. (
  • FF Science and Main Sequence are both deep technology funds that bring forward world-changing, science-based technologies. (
  • We're planning to modify human cells outside the body and then put them back into patients to treat a number of diseases, beginning with certain cancers and hematologic disorders," says Craig Mickanin, who leads a technology-based group at the Novartis Institutes for BioMedical Research (NIBR) within the Developmental & Molecular Pathways department. (
  • Neogene is committed to changing the treatment paradigm for solid cancers using our novel technology to provide access to tumor-specific TCRs for engineered T cell therapy on an individualized patient basis. (
  • Tapestri is the only technology on the market able to resolve every cell, every mutation and every edit. (
  • Malte is a co-founder and the CEO of CARTANA, a Stockholm-based ISS company that makes tissue ISS technology available to the global research community. (
  • His work on single molecule analysis and sequencing technologies is now part of CARTANA's technology portfolio. (
  • While Icahn School of Medicine and other consortium partners under DARPA's ECHO program generate WMD agent specific epigenetic signatures, INanoBio will develop its proprietary Field Effect Nanopore Transistor (FENT) technology for ultra-fast epigenetic sequencing. (
  • Organovo has been developing bioprinting technology, which lays down a pattern of cultured cells to create bio-engineered structures. (
  • Themis believes that today's licensing deal with Max-Planck-Innovation GmbH will further expand its innovative technology platform to include novel oncolytic virus-based immunotherapy applications. (
  • The single-cell RNA-sequencing analysis found that undifferentiated ependymomas are more likely to be linked to a poorer prognosis. (
  • Quantification of the DNA sequencing markets by product type, platform, customer type,value chain position, analysis type and geography. (
  • Another company, Ambry Genetics, also announced that it will be offering BRCA1 and BRCA2 analysis for approximately $2200. (
  • An in-depth analysis of the mobile applications of DNA sequencing, which is a developing personalized healthcare technique with enormous market potential. (
  • Advancements in the field of single-cell analysis are anticipated to boost the growth of the market in the coming years. (
  • This report analyzes the cell analysis market in terms of market revenue ($ million) for all segments. (
  • However as usually, stringent government regulations, lack of skilled personnel to perform some of cell analysis techniques like High Content Screening, NGS etc., and high cost of some of the commercial instruments are expected to hamper the market growth. (
  • The Cell analysis market is consolidated, with major players accounting for more than 80% market share in 2013. (
  • Life Technologies recognizes the increasingly important contribution of sequence-based analysis in the evolution of molecular medicine. (
  • Through Angle's existing research collaboration with the University of Surrey and the Royal Surrey County Hospital, the University of Surrey Oncology Department has already processed 20 colorectal cancer patient samples with the Parsortix system and stored the harvested cells for analysis. (
  • The TruePrime apoptotic cell free DNA amplification kit uses a novel multiple displacement amplification method to overcome the common limitations with the analysis of bodily fluid for cell free DNA analysis. (
  • Recent work has validated the platform using an advanced analysis method, single-cell RNA sequencing. (
  • Single cell analysis technologies have enabled the currently ongoing discovery and classification of cell types in tissues. (
  • A FENT array chip integrated with microfluidics will enable single cell analysis, reading out epigenetic patterns from tens of thousands of cells in a few minutes. (
  • The company amplies client's insight on the factors, such as strategies, future estimations, growth or fall forecasting, opportunity analysis, and consumer surveys among others. (
  • With developments in the analysis of circulating cancer cells and cell free DNA in peripheral blood they are poised to help in the screening of high risk individuals. (
  • The use next generation analysis of individual tumor cells including sequencing, mass spectrometry and PCR further enhance cancer testing in tissues. (
  • CTC analysis focuses on isolating and expanding populations of rare cells for downstream analysis. (
  • Purity of dendritic cells isolated from a fresh HemaCare leukopak, as measured by immunomagnetic cell separation analysis. (
  • To find his unicorn, Sinha would have to dig deeper, into the proteins that would eventually define the cells. (
  • Epigenetics refers to a heritable change that is not a result of a change in DNA sequence, but rather a chemical modification of nucleotides in the DNA or its associated proteins, Meyers said. (
  • Continuing to improve on that, the company intends to develop a panel of lines with improved capacity for manufacturing proteins. (
  • We anticipate that these findings will lead to the design of synthetic proteins, peptides or peptidomimetic compounds targeting cancer cells more specifically. (
  • DNA sequencing is also the most efficient way to sequence RNA or proteins (via their open reading frames ). (
  • The location of these markers varies in cells in different organs to ensure that proteins are only expressed where necessary. (
  • any of a group of five small basic proteins, occurring in the nucleus of eukaryotic cells, that organize DNA strands into nucleosomes by forming molecular complexes around which the DNA winds. (
  • Any of several proteins that, together with DNA, make up most of the chromatin in a cell nucleus. (
  • DNA is normally conceived of as a spiral ladder, but in eukaryotic cells (cells with nuclei) the DNA in the nucleus is strung around a series of spool-shaped proteins known as histones. (
  • Because these proteins enable cancer cells to anchor to new sites in the body, it may be possible to disrupt metastasis by blocking cadherin-mediated binding. (
  • The team first constructed ~10,000 base pair DNA sections and then ~100,000 base pair sections before stitching the final ~1 Mbp chromosome together. (
  • He has also held executive management positions with IBC Advanced Technologies, a molecular recognition company specializing in separations. (
  • with these new sequencing technologies, we now have the opportunity to acquire a comprehensive picture at fine detail, Meyers said. (
  • As iPS cell research grows in scale and moves closer to the clinic, investigators are increasingly in need of characterization standards that enable them to make informed decisions about the quality of their cells," said Chris Armstrong , Ph.D., General Manager and Vice President of Primary and Stem Cell Systems at Life Technologies. (
  • This announcement marks the third major license agreement in less than a year for Life Technologies' Primary and Stem Cell Systems group. (
  • In June 2012, the company retained the non-exclusive global rights from iPS Academia Japan for its iPS cell patent portfolio - enabling Life Technologies to expand its range of products and services. (
  • Life Technologies customers do their work across the biological spectrum, working to advance the fields of discovery and translational research, molecular medicine, stem cell-based therapies, food safety and animal health, and 21st century forensics. (
  • Many advances have been made in the characterization of immunoglobulins as a result of pharmaceutical companies investing in technologies that allow them to better understand MAbs during the development phase. (
  • Potential suitors, reported to include health-care companies, also have expressed interest in a Life Technologies buyout. (
  • Liquid biopsies (LBs) are an emerging, disparate group of technologies that seek to broaden the scope and sensitivity of blood-based cancer diagnosis. (
  • The kit enables accurate DNA amplification using cell free DNA (cfDNA) obtained from plasma, serum, urine, cerebrospinal fluid (CSF), or many other bodily fluids. (
  • So when 23andMe, a company that offers customers insight into their own DNA, opened up in the UK, he decided he'd give that a try too. (
  • Here, we found that siRNAs targeting mouse-specific lncRNA AA388235 could significantly induce death of human tumor cells, although it has no effect on mouse tumor cells and normal human cells. (
  • The mechanism studies showed that these siRNAs could activate the response of human tumor cells to exogenous nucleic acids, induce pyroptosis and apoptosis in the presence of GSDME, but induce apoptosis in the absence of GSDME. (
  • They also significantly inhibited the growth of human tumor cells in vivo . (
  • We are delighted to partner with Neogene, a company pioneering fully personalized T cell therapies for solid tumors," said Emily M. Leproust, Ph.D., CEO and co-founder of Twist Bioscience. (
  • Twist Biopharma, a division of Twist Bioscience, will create a specialized T cell receptor (TCR) library for Neogene with the goal of discovering engineered TCRs against two specified T cell targets in cancer for future Neogene personalized T cell therapies. (
  • Twist Bioscience is a leading and rapidly growing synthetic biology company that has developed a disruptive DNA synthesis platform to industrialize the engineering of biology. (
  • The conserved amino acid sequences designate the heavy chain isotype IgG, IgA, IgM, IgD, or IgE and the light chain either kappa or lambda. (
  • There are only two minor changes in the amino acid sequences of the histone designated H4 in the pea and cow, for example. (
  • The best current tool, built with components from one kind of bacterial CRISPR-Cas system, cuts both strands of the DNA molecule at a specific sequence, like adding a paragraph break to a block of text. (
  • DNA consists of two complementary strands of bases that "pair up" to make a double-stranded or duplex molecule. (
  • In the last few years, the biotech behemoth has acquired, invested in, partnered with, and spun out companies that can use its aggressively-patented sequencing tech to address disease. (
  • At the time of the announcement, Illumina had 49 NovaSeq orders, and since then machines have been installed in medical centers and precision medicine biotech companies around the world. (
  • The warning came as GeneWatch and The Guardian newspaper revealed exclusive deals between two US biotech companies and Japan Tobacco for the rights to develop and market new lung cancer vaccines (1,2). (
  • Much of the biotech companies' research has involved patient volunteers. (
  • UK company British Biotech has also received past payments from Japan Tobacco towards development of a genetically engineered treatment for heart attacks and strokes (7). (
  • 3) US biotech company Corixa is based in Seattle. (
  • Roche is the world's largest biotech company with truly differentiated medicines in oncology, virology, inflammation, metabolism and CNS. (
  • Ultimately, the goal of such sequencing in the awarded pilot project is to develop better cancer treatments while addressing fundamental questions in biology, according to Bielas, an associate member of the Public Health Sciences and Human Biology divisions at the Hutch. (
  • It took 1000 of the sequences to make the entire set of DNA that ultimately would be required to provide the instructions for asexual reproduction of the bacteria. (
  • Ultimately, the project hopes to set the stage for revolutions in treating genetically based disease. (
  • Following puromycin selection of stable lines of DSSP -silenced OSC2 cells, phenotypic hallmarks of oral carcinogenesis were assayed by western blot and RT-PCR analyses, MTT (cell-viability), colony-formation, modified Boyden-Chamber (migration and invasion), and flow cytometry (cell-cycle and apoptosis) analyses. (
  • For comparison, the gold standard flow cytometry method was only sensitive enough to spot residual cancer cells in 12 patients. (
  • North America dominates the global market for oligonucleotide synthesis due increasing R&D expenditure in biotechnology and pharmaceutical companies. (
  • In recent times, there is increased use of oligonucleotide synthesis due to increasing R&D expenditure in pharmaceutical and biotechnology companies. (
  • Increasing R&D investment of pharmaceutical companies would lead to growth in oligonucleotide synthesis market in Asia. (
  • Pharmaceutical companies have seen the development of peptides as a promising direction to lower their risk position. (
  • improved funding & aids for R&D activities, surge in partnership between academic, pharmaceutical, and biotechnology companies, and increased application of epigenetics in non-oncology diseases. (
  • San Diego-based Cylene Pharmaceuticals , a venture-backed bio-pharmaceutical startup developing first-in-class cancer drugs, has raised $867,000 of a planned $1 million financing, according to a recent regulatory filing . (
  • We believe our single-cell business will be the primary driver of WaferGen's long-term growth and, going forward, will be where our resources are primarily directed. (
  • Research & Development expenses increased $806,000 to approximately $2.3 million, compared to approximately $1.5 million in the June 2014 quarter, primarily as a result of increased activities related to the development and commercialization of our single cell products. (
  • Precise encoding plates can also be multiplexed, enabling up to 4,608 single cells to be prepared into one single sequencing library and reaction. (
  • Now a team from MIT, Harvard, and the Dana-Farber Cancer Institute in Boston has demonstrated a technique for isolating and quickly sequencing single snippets of DNA. (
  • Proteona is pioneering the use of validated multiplex panels of DNA-barcoded antibodies to provide proteomic information in addition to total mRNA sequence data from single cells. (
  • We have validated large sets of DNA barcoded antibodies that can be added to any single cell RNA sequencing experiment. (
  • This novel deep sequencing tool, which can decode tens of millions of sequences during a single run, has become available over the last year. (
  • However, a major challenge of this approach has been the need to dissociate the tissue into single cells. (
  • Then, a second device generates shear stresses to break tissue fragments into smaller units - from cell aggregates to clusters, and then finally to single cells - using an array of branching channels that repeatedly expand and constrict. (
  • Early tests have shown that the integrated device platform can extract more than 20,000 single, viable cells per milligram of tissue, which is two-fold to ten-fold greater than traditional methods. (
  • Upon pooling and cleanup, 384 samples are sequenced in a single Illumina MiSeq run. (
  • ONCOlogues are sensitive to a single base pair mismatch, resistant to degradation and use a proprietary delivery sequence to enter cells. (
  • Missing a single cell can be the difference of life and death," Charlie Silver, CEO and Co-founder of Mission Bio, told Vator. (
  • Leveraging an easy user interface and optimized primer design algorithms, custom targeted panels can be designed quickly and easily to complement Tapestri Single-Cell DNA Catalog Panels. (
  • Xiaoyan was involved in the development of probabilistic cell typing by ISS (pciSeq) method, a method to spatially map single-cell RNA-sequencing defined cell types. (
  • Dr. Takulapalli added "Another novel aspect of the ECHO program is its approach to profile epigenetic signatures at the single cell level, treating each cell as a sample. (
  • Perhaps the T cells could be modified further to improve efficacy in adults-and to extend the treatment beyond blood cancers to solid tumors. (
  • Ewing tumors, the second most frequent bone tumors in teenagers and young adults, show specific translocations fusing the 5′ part of EWS to the 3′ sequence encoding the DNA binding domain of an ETS factor [1] , [2] . (
  • In addition, microRNAs participate in the genesis and progression of tumors and play an important role in tumor cell proliferation, apoptosis, and migration [ 4 ]. (
  • With support from Uppsala and Stockholm-based innovation centers, Malte successfully spun out CARTANA from the Nilsson lab, now making ISS, which has been developed in the Nilsson lab over many years, available to the research community world-wide. (
  • 2) Japan Tobacco is the world's third largest tobacco company and makes 3 of the world's top 5 cigarette brands: Camel, Mild Seven and Winston. (
  • That same month, the company also announced a partnership with Cellular Dynamics International (CDI) , the world's largest producer of human cells derived from (iPS) cells, to commercialize a set of new products optimized to consistently develop and grow human iPS cells. (
  • So the tests associated with the drugs gefitinib (Iressa, AstraZeneca/Teva) and erlotinib (Tarceva/Roche, OSI Pharmaceuticals) have had lower market acceptance than the companies might have liked. (
  • Special Collections include microorganisms isolated from U. S. National Parks, a collection of strains and molecular reagents for yeast genetics research, and the Mantle Cell Lymphoma Cell Bank. (
  • Analyzing transcriptome data from more than 1,000 cells from 74 embryos, a Johns Hopkins University-led team found aneuploid cells in 80 percent of embryos. (
  • Poliovirus infects human cells by binding to an immunoglobulin-like receptor, CD155 (also known as the poliovirus receptor or PVR) on the cell surface. (
  • Following attachment to the host cell membrane, entry of the viral nucleic acid was thought to occur one of two ways: via the formation of a pore in the plasma membrane through which the RNA is then "injected" into the host cell cytoplasm, or via virus uptake by receptor-mediated endocytosis. (
  • The replication cycle of poliovirus is initiated (1) by binding to the cell surface receptor CD155. (
  • The idea is to map T-cell receptor sequences to the antigens they bind. (
  • These chimeric antigen receptor (CAR) T cells-ninjas by design-recognize a marker that's unique to the surface of cancer cells and launch an attack. (
  • Stem Cells Macrophages engulf damaged and dead cells to clear infection, but they also participate in tissue regeneration. (
  • Tissue engineering and regenerative medicine would also benefit from improving procurement of healthy and functional primary, progenitor, and stem cells from various organs and tissues to serve in tissue constructs and cell-based therapies. (
  • Uhlen M, Ponten F (2005) Antibody-based proteomics for human tissue profiling. (
  • Most of the new research in the field of regenerative medicine investigates the concept of stem cell-based tissue engineering approach for tissue regeneration [ 4 , 5 ]. (
  • In this way a chromosome containing 20 million base pairs of DNA is organized into approximately 100,000 nucleosome core particles. (
  • The residues of adenosines A180 and A181 in the GUAA tetraloop forms hydrogen bonds via non canonical base pairing interactions with the base pairs of the receptors C230/G242 and G231/C241, respectively. (
  • Given the improvements in read length to 2 × 250 base pairs, the Illumina MiSeq system seemed compatible for an amplicon sequencing approach as well. (
  • The ~1000 fragments of ~1000 base pairs each were "stitched" together inside yeast cells through a process of DNA recombination which is inherent to the cell. (
  • DNA synthesis, cloning, and recombination techniques are frequently used but have never been applied on the scale of millions of base pairs. (
  • In February, the company announced the enhancement of the Prequel system to include all 23 chromosome pairs rather than just the standard five chromosomes (13, 18, 21, X and Y), previously tested. (
  • The scope of the report includes NIPT methods, applications, patents and companies. (
  • He had just completed his post-doc in the lab of Irv Weissman, the Stanford biologist who helped launch the field of stem cells. (
  • That would require him to sequence the RNA of thousands of seemingly identical stem cells from a collection Weissman had built. (
  • Human and mouse stem cells available for purchase are listed in the ATCC Stem Cell Center along with protocols, FAQs, and links to online stem cell resources. (
  • The dots surrounding the H-shaped core are hollow portals through which implanted neural stem cells can extend axons into host tissues. (
  • If all goes well, the implants with neural stem cells could be ready for testing in human patients in a few years. (
  • Another study with neural stem cells without the implant has shown benefit in monkeys after spinal cord injury, Tuszynski said. (
  • These 2-millimeter-wide implants contain tiny channels that guide growth of neural stem cells, also called neural progenitor cells. (
  • The implants also protected the neural stem cells from the inflammatory damage associated with a fresh injury. (
  • An MRI shows stem cells labeled with iron oxide nanoparticles being injected into an animal's brain. (
  • Exocrine glands have been shown to be potential sources of pluri-/multipotent adult stem cells that are highly versatile as demonstrated for pancreas, testis, salivary glands, and sweat glands. (
  • AAVs are naturally occurring, derived from human stem cells. (
  • To sequence the DNA, each bead is placed in a tiny well, or "reactor," filled with nucleotides, the building blocks that make up DNA, as well as the chemical reagents that spur the building process. (
  • A computerized camera records the flashes and correlates them with the types of nucleotides washed across the well, calculating the sequence. (
  • DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. (
  • Various experimental procedures, including SELEX experiments and mapping of promoters regulated by EWS-FLI1, have shown that ETS factors bind purine-rich sequences with a GGAA/T core consensus sequence, surrounded by nucleotides that contribute to the specificity of each factor [8] - [11] . (
  • In one use case, Dr. Ravi Majeti at Stanford conducted several pilot studies using Mission Bio solutions, "garnering insights into clonal variation that couldn't be gathered using bulk sequencing techniques. (
  • Allied Market Research, a market research and advisory company of Allied Analytics LLP, provides business insights and market research reports to large as well as small & medium enterprises. (
  • The work, which was published in the June 29,2017 issue of Cell, provides new insights into cellular processes that regulate cell senescence and survival in aging and cancer. (