Colony-Forming Units Assay: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.Hematopoietic Stem Cells: Progenitor cells from which all blood cells derive.Clone Cells: A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Bone Marrow Cells: Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Interleukin-3: A multilineage cell growth factor secreted by LYMPHOCYTES; EPITHELIAL CELLS; and ASTROCYTES which stimulates clonal proliferation and differentiation of various types of blood and tissue cells.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Intensive Care Units: Hospital units providing continuous surveillance and care to acutely ill patients.Embryonic Stem Cells: Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.Hematopoiesis: The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).Colony-Stimulating Factors: Glycoproteins found in a subfraction of normal mammalian plasma and urine. They stimulate the proliferation of bone marrow cells in agar cultures and the formation of colonies of granulocytes and/or macrophages. The factors include INTERLEUKIN-3; (IL-3); GRANULOCYTE COLONY-STIMULATING FACTOR; (G-CSF); MACROPHAGE COLONY-STIMULATING FACTOR; (M-CSF); and GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; (GM-CSF).Tumor Stem Cell Assay: A cytologic technique for measuring the functional capacity of tumor stem cells by assaying their activity. It is used primarily for the in vitro testing of antineoplastic agents.Bone Marrow: The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.Cell Culture Techniques: Methods for maintaining or growing CELLS in vitro.Spermatogonia: Euploid male germ cells of an early stage of SPERMATOGENESIS, derived from prespermatogonia. With the onset of puberty, spermatogonia at the basement membrane of the seminiferous tubule proliferate by mitotic then meiotic divisions and give rise to the haploid SPERMATOCYTES.Cell Count: The number of CELLS of a specific kind, usually measured per unit volume or area of sample.Phytohemagglutinins: Mucoproteins isolated from the kidney bean (Phaseolus vulgaris); some of them are mitogenic to lymphocytes, others agglutinate all or certain types of erythrocytes or lymphocytes. They are used mainly in the study of immune mechanisms and in cell culture.Stem Cell Factor: A hematopoietic growth factor and the ligand of the cell surface c-kit protein (PROTO-ONCOGENE PROTEINS C-KIT). It is expressed during embryogenesis and is a growth factor for a number of cell types including the MAST CELLS and the MELANOCYTES in addition to the HEMATOPOIETIC STEM CELLS.Hematopoietic Cell Growth Factors: These growth factors comprise a family of hematopoietic regulators with biological specificities defined by their ability to support proliferation and differentiation of blood cells of different lineages. ERYTHROPOIETIN and the COLONY-STIMULATING FACTORS belong to this family. Some of these factors have been studied and used in the treatment of chemotherapy-induced neutropenia, myelodysplastic syndromes, and bone marrow failure syndromes.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Cell SeparationOctamer Transcription Factor-3: An octamer transcription factor that is expressed primarily in totipotent embryonic STEM CELLS and GERM CELLS and is down-regulated during CELL DIFFERENTIATION.Megakaryocytes: Very large BONE MARROW CELLS which release mature BLOOD PLATELETS.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Growth Substances: Signal molecules that are involved in the control of cell growth and differentiation.Immunotherapy, Adoptive: Form of adoptive transfer where cells with antitumor activity are transferred to the tumor-bearing host in order to mediate tumor regression. The lymphoid cells commonly used are lymphokine-activated killer (LAK) cells and tumor-infiltrating lymphocytes (TIL). This is usually considered a form of passive immunotherapy. (From DeVita, et al., Cancer, 1993, pp.305-7, 314)Immunotherapy: Manipulation of the host's immune system in treatment of disease. It includes both active and passive immunization as well as immunosuppressive therapy to prevent graft rejection.MuseumsTreatment Outcome: Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.Natural History: A former branch of knowledge embracing the study, description, and classification of natural objects (as animals, plants, and minerals) and thus including the modern sciences of zoology, botany, and mineralogy insofar as they existed at that time. In the 17th, 18th, and 19th centuries it was much used for the generalized pursuit of certain areas of science. (Webster, 3d ed; from Dr. James H. Cassedy, NLM History of Medicine Division)Transplantation, Homologous: Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals.Hematopoietic Stem Cell Transplantation: Transfer of HEMATOPOIETIC STEM CELLS from BONE MARROW or BLOOD between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Hematopoietic stem cell transplantation has been used as an alternative to BONE MARROW TRANSPLANTATION in the treatment of a variety of neoplasms.Murine Acquired Immunodeficiency Syndrome: Acquired defect of cellular immunity that occurs in mice infected with mouse leukemia viruses (MuLV). The syndrome shows striking similarities with human AIDS and is characterized by lymphadenopathy, profound immunosuppression, enhanced susceptibility to opportunistic infections, and B-cell lymphomas.Retroviridae Infections: Virus diseases caused by the RETROVIRIDAE.Leukemia Virus, Murine: Species of GAMMARETROVIRUS, containing many well-defined strains, producing leukemia in mice. Disease is commonly induced by injecting filtrates of propagable tumors into newborn mice.Retroviridae: Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).Anemia, Refractory: A severe sometimes chronic anemia, usually macrocytic in type, that does not respond to ordinary antianemic therapy.Erythropoiesis: The production of red blood cells (ERYTHROCYTES). In humans, erythrocytes are produced by the YOLK SAC in the first trimester; by the liver in the second trimester; by the BONE MARROW in the third trimester and after birth. In normal individuals, the erythrocyte count in the peripheral blood remains relatively constant implying a balance between the rate of erythrocyte production and rate of destruction.Erythroid Precursor Cells: The cells in the erythroid series derived from MYELOID PROGENITOR CELLS or from the bi-potential MEGAKARYOCYTE-ERYTHROID PROGENITOR CELLS which eventually give rise to mature RED BLOOD CELLS. The erythroid progenitor cells develop in two phases: erythroid burst-forming units (BFU-E) followed by erythroid colony-forming units (CFU-E); BFU-E differentiate into CFU-E on stimulation by ERYTHROPOIETIN, and then further differentiate into ERYTHROBLASTS when stimulated by other factors.Anemia, Sideroblastic: Anemia characterized by the presence of erythroblasts containing excessive deposits of iron in the marrow.Anemia, Refractory, with Excess of Blasts: Chronic refractory anemia with granulocytopenia, and/or thrombocytopenia. Myeloblasts and progranulocytes constitute 5 to 40 percent of the nucleated marrow cells.Erythropoietin: Glycoprotein hormone, secreted chiefly by the KIDNEY in the adult and the LIVER in the FETUS, that acts on erythroid stem cells of the BONE MARROW to stimulate proliferation and differentiation.Mesenchymal Stem Cell Transplantation: Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).Mesenchymal Stromal Cells: Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.Testis: The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.Adult Stem Cells: Cells with high proliferative and self renewal capacities derived from adults.Receptors, Aryl Hydrocarbon: Cytoplasmic proteins that bind certain aryl hydrocarbons, translocate to the nucleus, and activate transcription of particular DNA segments. AH receptors are identified by their high-affinity binding to several carcinogenic or teratogenic environmental chemicals including polycyclic aromatic hydrocarbons found in cigarette smoke and smog, heterocyclic amines found in cooked foods, and halogenated hydrocarbons including dioxins and polychlorinated biphenyls. No endogenous ligand has been identified, but an unknown natural messenger with a role in cell differentiation and development is suspected.Tetrachlorodibenzodioxin: A chemical by-product that results from burning or incinerating chlorinated industrial chemicals and other hydrocarbons. This compound is considered an environmental toxin, and may pose reproductive, as well as, other health risks for animals and humans.Myeloid Progenitor Cells: Stem cells derived from HEMATOPOIETIC STEM CELLS. Derived from these myeloid progenitor cells are the MEGAKARYOCYTES; ERYTHROID CELLS; MYELOID CELLS; and some DENDRITIC CELLS.
EpiCult-B media can assay for the presence of mammary precursor cells using either the mammary colony-forming cell (Ma-CFC) ... Methylcellulose-based medium is widely used to detect and quantify hematopoietic progenitor cells in the colony-forming unit ( ... and computer system designed specifically for automated imaging and counting of hematopoietic colonies in colony-forming unit ( ... STEMCELL Technologies Media for Hematopoietic Colony Assays MegaCult™ - Detect Megakaryocyte Progenitor Cells , STEMCELL ...
In this assay system, stromal cells were referred to as colony-forming unit-fibroblasts (CFU-f). The first clinical trials of ... Siminovitch L, Mcculloch EA, Till JE (1963). "The distribution of colony-forming cells among spleen colonies". Journal of ... Stromal cells are connective tissue cells that form the supportive structure in which the functional cells of the tissue reside ... The majority of modern culture techniques still take a colony-forming unit-fibroblasts (CFU-F) approach, where raw unpurified ...
... colony assay is designed to detect how many colony-forming-units of erythroid lineage there are in a hematopoietic tissue ... indicating that some cells in the colony have achieved the end-stage faster than others. Cell number in a colony is important ... CFU-E stands for Colony Forming Unit-Hematopoietic . It arises from CFU-GEMM (via BFU-E, which stands for "erythroid burst- ... forming units") and gives rise to proerythroblasts. Understanding the murine CFU-e assay (analogous to human assay): CFU-e is a ...
In microbiology, a colony-forming unit (CFU, cfu, Cfu) is a unit used to estimate the number of viable bacteria or fungal cells ... "Optimized digital counting colonies of clonogenic assays using ImageJ software and customized macros: comparison with manual ... and when counting colonies it is uncertain if the colony arose from one cell or a group of cells. Expressing results as colony- ... Counting with colony-forming units requires culturing the microbes and counts only viable cells, in contrast with microscopic ...
"Antiproliferative activity of Titanocene Y against tumor colony-forming units". Anticancer Drugs. 18 (3): 317-321. doi:10.1097/ ... Lümmen G, Sperling H, Luboldt H, Otto T, Rübben H (1998). "Phase II trial of titanocene dichloride in advanced renal-cell ... "Analyses of Titanocenes in the spheroid-based cellular angiogenesis assay". Toxicol In Vitro. 22 (2): 531-534. doi:10.1016/j. ... Titanocene Y can be given in the mouse in high dosages and it shows generally mild toxicity in the form of diarrhea. Titanocene ...
... it can be generally assumed that each cell will give rise to a single colony or Colony Forming Unit (CFU). The colonies can ... for instance the Luria-Delbrück experiment or the gentamicin protection assay). In addition, the enumeration of colonies on ... Measurements of cell viability, i.e. measuring and calculating the fraction of dead and live cells, for example of cells ... To quantify the number of cells in a culture, the cells can be simply plated on a petri dish with growth medium. If the cells ...
Siminovitch L, Mcculloch EA, Till JE (1963). "The distribution of colony-forming cells among spleen colonies". Journal of ... Properties of stem cells can be illustrated in vitro, using methods such as clonogenic assays, in which single cells are ... "Neurons derived from radial glial cells establish radial units in neocortex". Nature. 409 (6821): 714-20. doi:10.1038/35055553 ... mesenchymal stem cell, adipose-derived stem cell, endothelial stem cell, dental pulp stem cell, etc.). Muse cells (multi- ...
... in which colony forming units (CFU) on selective media are counted. Cultivating methods have several disadvantages. Culture- ... As a known component of indoor bioaerosol, β(1→3)-glucan (cell wall components of most fungi) is proposed to be the causative ... enzyme-linked immunosorbent assay (ELISA)). The well-known PCR is a powerful tool in identifying and even quantifying the ... Since PCR methods target DNA, viability of cells could not be confirmed in some cases. When qPCR technique is used for ...
The unit of measurement is cfu/ml (or colony forming units per millilitre) and relates to the original sample. Calculation of ... ATP is a molecule found only in and around living cells, and as such it gives a direct measure of biological concentration and ... samples contain a variety of components that can interfere with the ATP assay. The plate count method relies on bacteria ... Colonies that develop in the body of the medium can be counted by eye after incubation. The total number of colonies is ...
Titers of plaques can be found by diluting the samples and counting plaque-forming units (PFUs). Biochemical tests such as ... Plaque assays consist of pouring a soft agar solution with an indicator strain onto an agar plate. The indicator strain should ... UV light stresses lysogenic bacteria, leading the phages to propagate and burst the host bacterial cells. In the case of T12, ... In addition, none of the colonies containing the T12 genome was negative for speA, and therefore, the conclusion was drawn that ...
A form of replicate known as a dye flip can be performed to remove any dye effects in two-channel experiments; for a dye flip, ... Second, technical replicates (two RNA samples obtained from each experimental unit) help to ensure precision and allow for ... Microarray data is difficult to exchange due to the lack of standardization in platform fabrication, assay protocols, and ... Modules may corresponds to cell types or pathways. Highly connected intramodular hubs best represent their respective modules. ...
ISBN 978-0-470-47131-9. Gary, Ronald K. "Quantitative assay of senescence-associated β-galactosidase activity in mammalian cell ... Each unit of β-galactosidase consists of five domains; domain 1 is a jelly-roll type barrel, domain 2 and 4 are fibronectin ... The active ebg enzyme is an aggregate of ebgA -gene and ebgC-gene products in a 1:1 ratio with the active form of ebg enzymes ... After a time, certain colonies began to grow. However, the EbgA protein is an ineffective lactase and does not allow growth on ...
Colony forming or virtual colony count: e.g. by multiplying bacteria or proliferating cells. Photometry / spectrophotometry ... number of assays done per unit time (usually expressed as per hour) etc. Organizations or laboratories that perform Assays for ... MTT assay Cell Counting Kit-8 (WST-8 based cell viability assay) SRB (Sulforhodamine B) assay CellTiter-Glo® Luminescent Cell ... BCA assay) Bradford protein assay Lowry protein assay Secretion assay Nuclear run-on Ribosome profiling A cell-counting assay ...
1013 colony-forming units of phage. Following incubation, the phage are removed and the plate washed with buffer containing 0.5 ... An ongoing clinical trial is evaluating Zinc finger nucleases that disrupt the CCR5 gene in CD4+ human T-cells as a potential ... These assays are repeated using different target oligonucleotides. When investigating zinc fingers binding 5'-XNN-3' sequences ... After elution, the phage can be plated and DNA extracted from individual plaque forming units, restricted and the appropriately ...
Colony-forming unit-megakaryocyte (CFU-Meg) Colony-forming unit-basophil (CFU-B) Colony-forming unit-eosinophil (CFU-Eos) The ... A cobblestone area-forming cell (CAFC) assay is a cell culture-based empirical assay. When plated onto a confluent culture of ... Colony-forming unit-lymphocyte (CFU-L) Colony-forming unit-erythrocyte (CFU-E) Colony-forming unit-granulocyte-macrophage (CFU- ... which is a cell counting unit.) There are various kinds of HSC colony-forming units: Colony-forming unit-granulocyte- ...
Cell wall[edit]. Like other Gram-positive microbes, S. haemolyticus has a thick, rather homogenous, cell wall (60-80 nm) ... Detachment assays with NaIO4, proteinase K, or DNase result in 38%, 98%, and 100% detachment, respectively. The high level of ... The ability to adhere to medical devices and subsequently form biofilms is a major virulence factor associated with S. ... 2007). "Persistent strains of coagulase-negative staphylococci in a neonatal intensive care unit: virulence factors and ...
Of the 800 species of ticks, 40-64 species (across 10 genera) have been reported as causing a form of toxosis.[53][54][55][56] ... At the Royal North Shore Hospital in Sydney the Tick-borne Diseases Research Unit continues its study into this issue. Cases of ... Stone BF, Cowie MR , Kerr JD and Binnington KC (1982) Improved toxin/antitoxin assays for studies on the Australian paralysis ... The 30-day engorgement time is derived from laboratory culture colonies.) Under natural conditions, the time taken for an adult ...
Quality filtering of the reads can deliver higher raw accuracy reads which when aligned to form color contigs can deliver ... Therefore, since each color represents two nucleotides in which the second base of each dinucleotide unit constitutes the first ... Stem cell transcriptome profiling via massive-scale mRNA sequencing Rapid whole-genome mutational profiling using next- ... a bead or a planar solid surface Amplification of DNA fragments on the solid surface using PCR and making polymerase colonies ...
If 108 cells of a wild type strain are plated on agar plates containing phenyl-Gal, the rare colonies which grow are mainly ... The unphosphorylated form of EIIAGlc binds to the lac permease and prevents it from bringing lactose into the cell. Therefore, ... and is commonly used as a substrate for assay of β-galactosidase in vitro. Colonies that produce β-galactosidase are turned ... the colour change from white colonies to a shade of blue corresponds to about 20-100 β-galactosidase units, while tetrazolium ...
... by invading superficial umbrella cells to form intracellular bacterial communities (IBCs). They also have the ability to form K ... However, current latex assays and some typing antisera have shown cross reactions with non-E. coli O157 colonies. Furthermore, ... 1-40 units) Core region of phosphorylated nonrepeating oligosaccharides Lipid A (endotoxin) The O antigen is used for ... Cells are able to survive outside the body for a limited amount of time, which makes them ideal indicator organisms to test ...
... and enzyme systems in the liver cells begin to remove glucose molecules from strands of glycogen in the form of G6P. This ... The catalytic unit is associated with a calcium binding protein, and three transport proteins (T1, T2, T3) that facilitate ... Granulocyte colony-stimulating factor (G-CSF, e.g. filgrastim) therapy can reduce the risk of infection. Impaired platelet ... The diagnosis is definitively confirmed by liver biopsy with electron microscopy and assay of glucose-6-phosphatase activity in ...
Screening of donated blood, blood components, and solid organ donors, as well as donors of cells, tissues, and cell and tissue ... the disease progresses directly from the acute form to a symptomatic clinical form of chronic Chagas disease.[5][9] ... Even when colonies of insects are eradicated from a house and surrounding domestic animal shelters, they can re-emerge from ... including all units collected by the American Red Cross, which accounts for 40% of the U.S. blood supply.[38][39] The Chagas ...
Receptor-mediated endocytosis occurs next, providing the newly formed toxic complex access to the interior of the host cell. ... B. anthracis colonies are medium-large, gray, flat, and irregular with swirling projections, often referred to as having a " ... Anthrax was first tested as a biological warfare agent by Unit 731 of the Japanese Kwantung Army in Manchuria during the 1930s ... To confirm the organism is B. anthracis, rapid diagnostic techniques such as polymerase chain reaction-based assays and ...
Journal of Muscle Research & Cell Motility 6, 543-556. Loxdale, H.D., Hardie, J. Halbert, Susan, Foottit, R., Kidd, N.A.C. & ... However, as is known, aphids can produce sexual forms (males and sexual females = oviparae) from time to time, even on ... Loxdale, H.D. (1976) A method for the continuous assay of picomole quantities of ADP released from glycerol-extracted skeletal ... he then pursued comparative studies for a DPhil in the ARC Unit of Insect Physiology & Muscle Mechanisms, Zoology Department, ...
Most slippage results in a change of just one repeat unit, and slippage rates vary for different allele lengths and repeat unit ... Hence a tumour cell line might show a different genetic fingerprint from that of the host tissue, and, especially in colorectal ... An archaic form of splicing preserved in Zebrafish is known to use microsatellite sequences within intronic mRNA for the ... Colonies are then developed, and screened with fluorescently-labelled oligonucleotide sequences that will hybridize to a ...
On the second day of battle, a unit of the XIX Corps was forced to halt when it ran out of gasoline.[39] One of the major ... Shortening gasoline to gas, which happens often, causes confusion with various forms of gaseous products also used as ... according to the Petroleum Planning and Analysis Cell of the Ministry of Petroleum.. ... though this varies based on the crude oil assay. ... navy to protect the shipping of raw materials from its colonies ...
... although the number of phenotypic hematopoietic stem cells (HSCs) in Setd2-deleted mice is unchanged, functional assays, ... Gene expression profile of Setd2-deleted hematopoietic stem/progenitor cells (HSPCs) partially resembles that of Dnmt3a/Tet2 ... which results in proliferation and cell cycle abnormalities and genomic instability, allowing accumulation of secondary ... n = 4. e Colony-forming unit (CFU) assay of total BM cells. n = 3. f, g Representative FACS analysis (f) and absolute cell ...
Culture Colony-Forming Unit Assay.. Frontoparietal bones, femora, and brains of five mice were ground using a mortar and pestle ... Cells.. The murine progenitor cell lines factor-dependent cells Paterson-mix (FDCP-mix) and M1 were grown as previously ... Fluorescent labeling with BCECF was as described for FDCP-mix cells.. Unlabeled aliquots of cell lines and FL cells were ... pathway and one or more unidentified lectins determine in part the recovery of colony-forming units from the BM of irradiated ...
Colony-forming unit assay. A colony-forming unit (CFU) assay was carried out to show blast elimination in a long-term coculture ... In ELISpot assays at a 1:1 ratio of leukemia cells to T cells, the TAA-specific T cells showed increased IFN-γ-release (mean, ... relative inhibition of colony formation of leukemic cells in CFU assays when cocultured with specific T-cell lines (n = 8; CTL ... In contrast to virus-specific T cells where memory T cells are the main source of expanded T cells, naïve T cells seem to be an ...
Colony forming unit-fibroblast assay. CFU-F assays were performed by plating either 1×105 unselected or 500-5,000 FACS-selected ... MSC can be identified by their ability to form colony forming units-fibroblast (CFU-F) in vitro.2,7 However, these cells are ... The growth of mesenchymal stem cells was monitored by colony formation unit fibroblast (CFU-F) assays. The differentiation of ... cells and RS cells and between MSCA-1+CD56− cells and mMSC can be drawn. Thus, MSCA-1+CD56+ cells reside in the FSlo-medium/SS ...
The hD1R-expanded cells had the enhanced homing and maintained long-term hematopoietic stem cell repopulation capacity in the ... Moreover, systemic administration of hD1R promoted the in vivo regeneration of donor cells in recipient mice and accelerated ... and tested the effects of this protein on human umbilical cord blood hematopoietic stem and progenitor cell (UCB-HSPC) ex vivo ... has been severely limited by low and finite numbers of hematopoietic stem cells and their delayed engraftment. New strategies ...
Colony-forming units assay‎ (21 F). *. ► Cytogenetics‎ (4 C, 21 F). *. ► Cytological techniques‎ (3 C, 54 F) ... Colonies of Madin-Darby Canine Kidney cells grown in tissue culture.jpg 1,030 × 1,030; 263 KB. ... cell biology (sco); Эсийн биологи (mn); cellebiologi (nn); cytologi (nb); sitologiya (az); cell biology (en); علم الأحياء ... Comparison of single cell methylation sequencing methods in terms of coverage as at 2015.png 823 × 871; 112 KB. ...
Chemical biology of the cell‎ (1 C). *. ► Chemiosmosis‎ (4 F). *. ► Colony-forming units assay‎ (21 F) ... Colonies of Madin-Darby Canine Kidney cells grown in tissue culture.jpg 1 030 × 1 030; 263 KB. ... biología celular (es); frumulíffræði (is); biologi sel (ms); cell biology (en-gb); Клетъчна биология (bg); Cell biology (simple ... cell biology (sco); Эсийн биологи (mn); cellebiologi (nn); cytologi (nb); sitologiya (az); cell biology (en); علم الأحياء ...
... biological response modifiers and the family of cell adhesion-promoting molecules. ... Colony-Forming Unit Assays. Single-cell suspensions from the spleen and BM were prepared at 1 × 106 and 2 × 105 cells/mL, ... The cultures were incubated at 37°C, 5% CO2 in air, and 95% humidity for 8 days after which Colony-Forming Unit Macrophages ( ... 0.001, resp.). Analysis of progenitors Colony Forming Unit-Macrophage (CFU-M) did not reveal major modifications in the BM of ...
The clonal make-up of the haematopoietic system of mice reconstituted with retrovirus-infected bone marrow cells was analysed ... Colony-Forming Units Assay * Embryo, Mammalian * Fluorouracil / pharmacology * Hematopoiesis * Hematopoietic Stem Cells / ... Clonal fluctuation within the haematopoietic system of mice reconstituted with retrovirus-infected stem cells EMBO J. 1987 Dec ... The clonal make-up of the haematopoietic system of mice reconstituted with retrovirus-infected bone marrow cells was analysed ...
Colony-forming Units Assay. A cytologic technique for measuring the functional capacity of stem cells by assaying their ... Each colony (i.e., microbial colony-forming unit) represents the progeny of a single cell in the original inoculum. The method ... Home » Topics » Myc the protein encoded by the MYC gene that activates the cell cycle » Research » Colony-Forming Cell Assay ... Summary of "Colony-Forming Cell Assay Detecting the Co-Expression of JAK2V617F and BCR-ABL1 in the Same Clone: A Case Report." ...
Here, we describe a plasma poor pathogen-free platelet lysate obtained by pooling 12 platelet (PLT) units, to produce a ... PL lots were obtained by combining 2 6-unit PLT pools in additive solution (AS) following a transfusional-based procedure ... are required to promote growth and expansion of these cells. Platelet lysate (PL) is a human blood component which may replace ... and peripheral blood mononuclear cells (PBMC) mitogen induced proliferation. PI-PL and PL control lots had similar ...
... and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and ... Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, ... Figure 2: Colony forming unit assay of eBM-MSCs. Representative image of CFU formed after 10 days of seeding passage 1 eBM-MSCs ... 2.3.1. Colony Forming Unit Assay (CFU). Passage 1 of equine MSCs was seeded at a density of 1 × 106 in 100 mm tissue culture ...
... a discrepancy is observed between the decreased in vitro erythroid colony formation and the normal or increased number of ... Bone Marrow Cells / pathology*. Cells, Cultured. Colony-Forming Units Assay. Erythropoiesis*. Erythropoietin / blood. ... Previous Document: Quality of IL-3 and G-CSF-mobilized peripheral blood stem cells in patients with early chronic phase.... ... Hematopoietic Stem Cells / pathology*, physiology. Humans. Leukocyte Count. Middle Aged. Platelet Count. Receptors, Transferrin ...
A putative mesenchymal stem cells population isolated from adult human testes R. Gonzalez a , L. Griparic a , V. Vargas a , K. ... Colony-forming units (CFU) assay and cell cloning. For colonies, cells were plated at a density of 150 cells/10 cm dish. After ... Supernatant containing spermatogonial cells and dying cells were discarded. Attached cells were fed every 3-4 days. Under these ... Cell differentiation Adipogenic differentiation. Cells were plated in 12-well plates. At 90-100% confluency cells were switched ...
Suppression of programmed cell death is also thought to be critical for this process; however, the link between these two ... Colony-Forming Units Assay * DNA-Binding Proteins / physiology* * Embryonic Stem Cells / metabolism ... Suppression of programmed cell death is also thought to be critical for this process; however, the link between these two ... This programmed cell death is Arf and p53 independent and is instead mediated by upregulation of the proapoptotic factor Bim. ...
The Translational Trial Development and Support Laboratory at Cincinnati Childrens supports clinical assay development from ... technology developed in academic laboratories and provides assay development and testing support for early phase clinical ... Hematopoietic cell colony forming unit (CFU) assay. * Protein HPLC. * Gene expression by qPCR or flow cytometry ... We also provide assay development and testing support for early phase clinical trials with a focus on cell and gene therapy. We ...
Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological ... The Granulocyte/Macrophage Colony-Forming Unit Assay Augusto Pessina. Pages 217-230 ... Colon Monoclonal Antibodies cell cell culture cell cycle cell differentiation cells cytokine gene expression gene therapy ... The Human Leukemia Cell Line HL-60 as a Cell Culture Model To Study Neutrophil Functions and Inflammatory Cell Responses ...
Bone Marrow Cell ColonyForming Unit Assays.. Freshly isolated bone marrow cells were resuspended at concentrations of 105 cells ... and used to prepare progenitor lymphoid cell (CFU-pre-B) and progenitor myeloid cell (CFU-GM) colony forming assays (B and C). ... and used to prepare progenitor lymphoid cell (CFU-pre-B) and progenitor myeloid cell (CFU-GM) colony forming assays (B and C). ... Colony-forming unit assays show suppression of lymphoid progenitors by each PAH within 6 h but a subsequent recovery, ...
The major role of stem cells is to restore of injured tissues. Since the discover of stem cells, they gained a big... ... Stem cells are undifferentiated cells located in different parts of the body. ... Bone marrow stem cells. CFU-F. Colony forming unit fibroblast assay. Col-I. Collagen-I ... Dental tissues Stem cells Dental stem cells Mesenchymal stem cells Abbreviations. ADSCs. Stem cells derived from adipose tissue ...
MethoCult™ Media for Performing Hematopoietic Colony-Forming Unit (CFU) Assays Product Type:. Cell Culture Media and ... Hematopoietic Stem and Progenitor Cells (HSPCs): Isolation, Culture, and Assays Cell Type:. Hematopoietic Stem and Progenitor ... ClonaCell™-CHO ACF Supplement for Robust Growth of CHO Cells at Low Cell Density Product Type:. Cell Culture Media and ... ClonaCell™-HY Medium D for Robust Colony Formation During Semi-Solid Cloning Product Type:. Cell Culture Media and Supplements ...
Colony-forming unit (CFU) assay. Bone marrow cells were harvested from BALB/c mice (The Jackson Lab, #000651), Flt3ITD and Flt3 ... b Representative images of colony-forming unit (CFU) assay demonstrate reduced crenolanib sensitivity of Flt3ITD;Tet2+/− mouse ... colony counting software (StemCell Technologies). Colony cells were harvested, stained with cell surface markers and analyzed ... Inhibitor assay. Transformed Ba/F3 cells were seeded in 384-well plates (1250 cells per well) and exposed to increasing ...
... and plays an important role in cell survival, differentiation, and apoptosis. To investigate its role in cell fate ... Colony-Forming Units Assay. Enzyme Activation. Extracellular Signal-Regulated MAP Kinases / metabolism*. Flow Cytometry. G1 ... apoptotic in both SK-N-MC and U2OS cells. Furthermore, the truncated form (p18) of Bax accumulated in the TrkA-induced cells, ... TrkA-mediated cell death was shown by the annexin-V binding assay to be, at least in part, ...
... isolation and purificationQuality assurance Differentiation assays for MSCColony forming units assayCell surface markers (FACS ... NESCC is a part of the infrastructure to support and conduct basic and clinical research related to stem cells for members of ... Radhika Pochampally, PI(601) 815-6832Patrice Penfornis, Core Manager(601) 984.2165Non-Embryonic Stem Cell CoreUniversity of ... the academic research community.ServicesStem cell expansion and EVs productionNormal mesenchymal stem cells (MSCs)Cancer stem ...
Bone marrow colony forming units assay. Bone marrow cells were obtained by flushing femurs and tibiae with 1 mL of Iscoves ... Colonies were scored for granulocyte-macrophage colony-forming unit (CFU-GM), colony-forming unit-granulocyte, erythrocyte, ... Bone marrow cell suspensions were diluted to 2 × 105 cells/mL in IMDM 2% FCS medium, and 0.3 mL of cells were mixed with 3 mL ... we asked whether amino-bisphosphonate affects the number of clonogenic colony-forming progenitor cells present in bone marrow. ...
However, the host cells in turn deploy a multipronged defense mechanism to control bacterial infection. Endoplasmic reticulum ( ... Pre-treatment of Raw 264.7 cells with 4-PBA (an ER stress-inhibitor) reduced the activation of the ER stress indicators, ... Pre-treatment of Raw 264.7 cells with 4-PBA (an ER stress-inhibitor) reduced the activation of the ER stress indicators, ... However, the host cells in turn deploy a multipronged defense mechanism to control bacterial infection. Endoplasmic reticulum ( ...
  • However, contradictory evidences demonstrate that inhibition of osteoclast activity by bisphosphonate does not impair HSPC mobilization in response to Granulocyte-Colony Stimulating Factor (G-CSF) treatment [ 1 , 10 ], suggesting that osteoclasts may only intervene in certain types of hematopoietic stresses. (hindawi.com)
  • In granulocyte colony-stimulating factor-mobilized autologous peripheral blood stem cells, the FISH ratio of interphase XYY cells to XY cells was 1.64, and there were 3.4 times more G-banded XYY metaphases than normal metaphases. (nih.gov)
  • Moreover, systemic administration of hD1R promoted the in vivo regeneration of donor cells in recipient mice and accelerated hematopoietic recovery, particularly in settings wherein the HSPCs dose was limiting. (biomedcentral.com)
  • To study the in vivo and in vitro erythropoiesis in more detail erythron transferrin uptake (ETU), soluble transferrin receptor (sTfR) and erythroid in vitro colony formation were performed in 24 patients with RA and five patients with RARS. (biomedsearch.com)
  • Bone marrow (BM) hematopoietic cells are selectively sensitive to polycyclic aromatic hydrocarbons (PAH) in vivo. (aspetjournals.org)
  • To investigate the mechanism by which CAPE enhanced HSPC homing, we performed these experiments including Q-PCR, western blot, immunohistochemistry and CFU assays after in-vivo HIF-1α activity blockade. (biomedcentral.com)
  • To determine the effect of PIO (pioglitazone) on BMSC (bone marrow stem cell) lineage choice in vivo, a bone marrow aspiration was obtained from patients at baseline and after 6 months of treatment with PIO or placebo. (clinicaltrials.gov)
  • The bone marrow was used for ex vivo CFU-OB (Colony forming units-Osteoblast) and CFU-AD assays using the same protocol described for the in vitro studies previously. (clinicaltrials.gov)
  • An ex vivo assay for examining the clonogenic potential of multipotent marrow cells was later reported in the 1970s by Friedenstein and colleagues. (wikipedia.org)
  • However, reverse‐transcribed LV DNA did trigger p53 signaling, activated also by non‐integrating Adeno‐associated vector, ultimately leading to lower cell recovery ex vivo and engraftment in vivo . (embopress.org)
  • Additionally, CD4 + T cells from TSLPR KO mice expanded less efficiently than WT CD4 + T cells in irradiated hosts, and TSLP preferentially expanded CD4 + T cells both in vitro and in vivo. (rupress.org)
  • Finally, carboxyfluorescein succinimidyl ester (CFSE)-based experiments showed TSLP-dependent expansion of CD4 + T cells both in vitro and in vivo. (rupress.org)
  • However, this assay does not recapitulate the intricacies of hematopoiesis in vivo , nor does it replace in vivo testing in mice. (taconic.com)
  • developed a novel in vivo myelotoxicity assay using hGM-CSF/hIL3 transgenic NOG mice humanized with cord blood derived CD34+ cells ( huNOG-EXL ). (taconic.com)
  • Additionally, in transplant and serial transplant experiments, using our transgenic CML mouse model, we could subsequently show that IFX therapy boosted TKI-induced effects and further reduced the proportion of malignant stem cells in vivo. (biomedcentral.com)
  • Adipose derived- and bone marrow-derived murine mesenchymal stem cells (mMSCs) may be used to study stem cell properties in an in vivo setting for the purposes of evaluating therapeutic strategies that may have clinical applications in the future. (biomedcentral.com)
  • Ex vivo expansion experiments were carried out using freshly purified CB CD34 + cells in StemSpan™ SFEM medium in the presence of stem cell factor, Flt3-Ligand and thrombopoietin at 50 ng/ml. (biomedcentral.com)
  • Blood stage malaria causes systemic inflammation and acute hemolytic anemia in mice [ 20 ], which are events that trigger stress hematopoiesis to generate phagocytic cells involved in parasite clearance and new erythrocytes to cope with anemia [ 21 - 24 ]. (hindawi.com)
  • Hematopoiesis can be recapitulated in vitro by using colony-forming unit (CFU) assays that specifically provide for the expansion of each progenitor type. (aspetjournals.org)
  • BALB-neuT mice expressing an activated rat c-erbB-2/neu transgene under the mouse mammary tumor virus long terminal repeat show enhanced hematopoiesis with hyperproduction of myeloid-derived suppressor cells (MDSC) because of vascular endothelial growth factor (VEGF) secreted by the tumor. (aacrjournals.org)
  • In BALB-neuT mice, vascular endothelial growth factor (VEGF) released by the tumor is the main factor that conditions hematopoiesis toward the massive expansion of a Gr1/CD11b double-positive, immature myeloid cell population ( 2 ). (aacrjournals.org)
  • Hematopoiesis is a highly orchestrated process involving multilineage blood cell development. (bloodjournal.org)
  • Hematopoiesis is the physiological process where hematopoietic stem cells (HSCs) continuously generate the body's complement of blood and immune cells within unique regions of the bone marrow termed niches. (sciencemag.org)
  • Hematopoietic stem cells (HSCs) are responsible for hematopoiesis, the production of the full complement of blood and immune cells ( 1 ). (sciencemag.org)
  • Setd2 deficiency also induces DNA replication stress in HSCs, as reflected by an activated E2F gene regulatory network and repressed expression of the ribonucleotide reductase subunit Rrm2b, which results in proliferation and cell cycle abnormalities and genomic instability, allowing accumulation of secondary mutation(s) that synergistically contributes to tumorigenesis. (nature.com)
  • However, due to difference in the cytokines used and loss of long-term engraftable cells in vitro expansion, an effective protocol for the expansion of UCB HSCs is still to be explored. (biomedcentral.com)
  • Quiescent hematopoietic stem cells (HSCs) reside in the bone marrow (BM) near the endosteum, in specific microenvironments called endosteal niches [ 1 - 3 ]. (hindawi.com)
  • The defect in HSCs/HPCs caused by Apc ablation is cell autonomous. (rupress.org)
  • However, the Cdkn1a and Cdkn2c cell cycle inhibitors have an opposing role in regulating the self-renewal of HSCs ( 14 ). (rupress.org)
  • In a classical view, the earliest cells in the hematopoietic hierarchy are the hematopoietic stem cells (HSCs). (bloodjournal.org)
  • 17 Recently, by conditional gene targeting in mice our group has shown that Cdc42 is required for the homing, lodging, and engraftment, as well as the maintenance of a quiescent cell cycle state, of long-term HSCs in the bone marrow microenvironment. (bloodjournal.org)
  • Recent data suggest that the endosteal niche, marked by a rich population of premature osteoblastic cells and arteriolar structures near the bone surface, houses quiescent, long-term repopulating HSCs ( 2 ). (sciencemag.org)
  • Given the fact that day-14 and day-35 CAFCs are functional surrogates of HPCs and hematopoietic stem cells (HSCs), respectively, these results suggest that dual inhibition of mTORC1/2 may have distinct effects on HPCs versus HSCs. (sigmaaldrich.com)
  • HSCs are adult tissue stem cells found naturally primarily in the bone marrow and in umbilical cord blood. (regmednet.com)
  • HSCs function to initiate and sustain production of the many different mature blood cells, including red blood cells, white blood cells, and platelets, that must be continuously replenished throughout life. (regmednet.com)
  • Novel modulators of HSCs fate continue therefore to be identified in an attempt to optimize the in vitro culture of these cells. (alliedacademies.org)
  • Selectin-independent rolling was mediated by α4 integrins, which interacted with endothelial vascular cell adhesion molecule (VCAM)-1. (rupress.org)
  • HPCs were also shown to bind to P-selectin (( 13 )), although the expression of P-selectin on BM endothelial cells has not been described. (rupress.org)
  • Because it remodels the extracellular matrix and promotes sprouting and growth of new blood vessels by making VEGF available to the VEGFR-2/flk receptor on endothelial cells, MMP-9 is also a linchpin in tumor progression ( 15 - 18 ). (aacrjournals.org)
  • First, infused HSPCs find their way to bone marrow (BM) microvessels via rolling and firm adhesion to endothelial cells. (biomedcentral.com)
  • Subsequently, the HSPCs migrate across the BM endothelial cells (BMECs) and extracellular matrix barrier and lodge in the BM niche. (biomedcentral.com)
  • Bone marrow derived endothelial progenitor cells (EPCs) are mobilized and are homed to sites of myocardial injury to help with repair of damaged myocardium. (aacrjournals.org)
  • In the setting of Hh inhibition, induction of either Notch signaling or overexpression of Stem cell leukemia (Scl)/T-cell acute lymphocytic leukemia protein 1 rescued hemogenic vascular-endothelial cadherin + cells and hematopoietic progenitor formation. (zfin.org)
  • Recent studies suggest that circulating exogenous endothelial progenitor cells (EPCs) play an important role in endothelial repair and neovascularization at sites of injury or ischemia. (ahajournals.org)
  • 2 , 5 - 7 The depletion and dysfunction of the circulating endothelial progenitor cells (EPCs) are thought to underlie the endothelial dysfunction in DM. (ahajournals.org)
  • In the search phrases used, the following terms were combined with the phrase AND Diabetes: endothelial progenitor cells, cardiovascular disease, myocardial infarction, and stroke. (ahajournals.org)
  • The laser/photosensitizer combinations Er.Cr:YSGG/TBO and GaAs/ADP will be applied to cariogenic bacteria, cariogenic plaque and gingival fibroblasts, epithelial and endothelial cells, plus three control groups: (1) suspension fluid without TBO or ADP, not irradiated, (2) suspension(s) fluid without TBO or ADP, irradiated for different periods, and (3) suspension fluid with TBO or ADP, not irradiated. (nova.edu)
  • Subsequently, when a rolling cell encounters a chemoattractant that binds to surface receptors, an intracellular signaling cascade is triggered that induces the functional upregulation of integrins. (rupress.org)
  • RPE cells robustly controlled intracellular outgrowth of Mtb early after infection. (arvojournals.org)
  • Formalin-killed R. salmoninarum also escaped into the cytoplasm, albeit at a slower rate and after sustaining greater cell wall damage, suggesting that the extracellular protein of the bacterium plays a role in intracellular survival. (int-res.com)
  • It acts as an intracellular signal transducer in cells by cycling between the active, GTP-bound form and the inactive, GDP-bound form in response to diverse stimuli. (bloodjournal.org)
  • Intracellular survival assay was performed by infecting RAW264.7 cells with M. smegmatis before and after treatment with plant extracts. (biomedcentral.com)
  • Gemfibrozil (GFZ) blocks organic anion transport in J774 cells, thereby facilitating the intracellular accumulation of NFX (Cao, C., H.C. Neu, and S.C. Silverstein. (rupress.org)
  • To determine whether GFZ enhances the efficacy of fluoroquinolone antibiotics against intracellular bacterial pathogens, J774 cells were infected with Listeria monocytogenes and incubated in medium containing a fluoroquinolone antibiotic in the presence or absence of GFZ. (rupress.org)
  • Intracellular growth of L. monocytogenes was evaluated by lysing J774 cells and assaying for colony-forming units of Listeria. (rupress.org)
  • 99% of J774 cells contained intracellular L. monocytogenes at 14 h after infection. (rupress.org)
  • Therefore, a single microRNA can target multiple different proteins that coordinate the same pathway in the cells that are critical for clotting and hence for human health. (elifesciences.org)
  • In developing white matter, decreased total β-catenin, activated β-catenin, and cyclin D1 levels coincided with the peak of Sox17 expression, and immunoprecipitates showed a developmentally regulated interaction among Sox17, T-cell transcription factor 4, and β-catenin proteins. (jneurosci.org)
  • High-mobility group (HMG) proteins are involved in oligodendrocyte lineage specification and cell maturation ( Wegner, 2000 , 2001 ). (jneurosci.org)
  • Plasma contains proteins, but no stem cells, so it is not needed for transplant therapy. (smartcells.com)
  • After injury, the blood-nerve barrier permeability increases and an inflammatory response is initiated resulting in proliferation of Schwann cells and activation of local macrophages that respond to tissue damage [ 2 , 3 ]. (hindawi.com)
  • Macrophages are the major cell type infected by Mtb and M. bovis . (frontiersin.org)
  • To address a potential role for Mtb -infected RPE cells in the development of uveitis, we delineated the response to Mtb infection in human RPE cells and primary human macrophages, the main target cell of Mtb . (arvojournals.org)
  • Primary human RPE cells, the human RPE cell line ARPE-19, and monocyte-derived proinflammatory M1 and anti-inflammatory M2 macrophages were infected with DsRed-expressing Mtb strain H37Rv. (arvojournals.org)
  • In contrast, macrophages engaged a plethora of responses including IFN signaling and communication between innate and adaptive immune cells to induce granuloma formation. (arvojournals.org)
  • These results suggest that GFZ could be used clinically to enhance the efficacy of fluoroquinolone and of other anionic antibiotics against bacteria that grow and/or reside within macrophages and/or other cells. (rupress.org)
  • Understanding the differences in bone progenitor cell activity and recruitment during osteoporosis therapy will provide a mechanistic rationale for effective use of PTH and anti-resorptive drugs in osteoporosis treatment. (clinicaltrials.gov)
  • Quantitation of progenitor cell populations and growth factors after bone marrow aspirate concentration. (regenexx.com)
  • Likewise to human CML, TNFα signaling was specifically active in murine CML stem cells, and ectopic expression of Bcr-Abl in murine and human progenitor cell lines induced TNFα expression. (biomedcentral.com)
  • Although previous investigations have revealed gradients in cellular and extracellular matrix (ECM) content across the marrow, and matrix elasticity and ligand type are believed to be strong regulators of stem cell fate, the impact of biophysical signals on HSC response is poorly understood. (sciencemag.org)
  • The cells, which are long and thin, are widely dispersed and the adjacent extracellular matrix is populated by a few reticular fibrils but is devoid of the other types of collagen fibrils. (wikipedia.org)
  • Gene expression profile of Setd2 -deleted hematopoietic stem/progenitor cells (HSPCs) partially resembles that of Dnmt3a/Tet2 double knockout HSPCs, showing activation of the erythroid transcription factor Klf1-related pathway, which plays an important role in hematopoietic malignant transformation. (nature.com)
  • Colony-forming assays demonstrated the coexistence of 2 different haematopoietic clones: one was positive for the JAK2V617F mutation and the other co-expressed both JAK2V617F and the BCR-ABL1 fusion gene. (bioportfolio.com)
  • We also provide assay development and testing support for early phase clinical trials with a focus on cell and gene therapy. (cincinnatichildrens.org)
  • The TTDSL has effectively supported 11 Phase I/II gene therapy clinical trials by designing tailored analytical assays and acquiring sponsor-developed assays via technology transfer. (cincinnatichildrens.org)
  • We determined whether CAPE has a novel function in improving the homing and engraftment of haematopoietic stem/progenitor cells (HSPCs) by regulating HIF-1α gene expression in the bone marrow (BM) niche. (biomedcentral.com)
  • 6 - 9 Ectopic expression or gene knockout studies of specific transcription factors have provided convincing evidence that blood cell lineage fate, such as myeloid, erythroid, or lymphoid commitment, can be switched by altered expression patterns of transcription factors including PU.1, C/EBPα, Gfi-1, and/or GATA2. (bloodjournal.org)
  • By gene expression microarrays, we observed that restitution of B2M in LC cells up-regulated targets of IFNalpha/IFNgamma. (cnio.es)
  • focus on one specific microRNA gene, which is vital for the production and the function of several classes of blood and immune cells. (elifesciences.org)
  • Encouraging clinical results of recent hematopoietic stem cell (HSC) gene therapy trials are opening novel perspectives for the treatment of monogenic diseases affecting the hematopoietic system. (embopress.org)
  • Single-cell RNA-sequencing technology recently identified an enriched inflammatory gene signature with TNFα and TGFβ being activated in TKI persisting quiescent LSC. (biomedcentral.com)
  • Using this approach, the authors identified a malignant stem cell population reflecting a gene signature associated with either cycling or quiescence, with the latter population persisting in patients despite therapy. (biomedcentral.com)
  • Several reporter gene or tracking strategies are currently being developed to assist investigators with experimental stem cell therapies. (biomedcentral.com)
  • Culture-expanded CD271+ cells were analyzed for tripotentiality and gene expression. (whiterose.ac.uk)
  • We observed that cell aggregation in suspension promoted cell survival and proliferation. (nih.gov)
  • Analysis of tyrosine kinase-mediated cell survival and growth signaling pathways revealed increased levels of tyrosine-phosphorylation of PECAM-1 and Pyk2 in cell aggregates. (nih.gov)
  • In order to understand the molecular nature of the cell aggregation-generated survival and growth signals, we analyzed the phosphorylation/activation of PECAM-1 and Pyk2. (nih.gov)
  • The response in RPE cells to control Mtb survival was dominated by interferon (IFN) signaling and further characterized by prominent regulation of cell death/survival-associated genes and low-level production of Th1-associated cytokines. (arvojournals.org)
  • The durability of its cell wall enhanced survival within MP and significant bacterial losses occurred only after 96 h as the MP died and exposed the bacteria to antibiotics in the media. (int-res.com)
  • Our results suggest that leukemia microvesicles are able to induce some effects on healthy hematopoietic stem cells such as promoting cell survival and some microRNAs deregulation, while stemness is maintained. (cancerindex.org)
  • Thus, as compared with other known cytokines, TSLP is distinctive in exhibiting a lineage preference for the expansion and survival of CD4 + T cells. (rupress.org)
  • Wnt (Wingless and integration site) factors are secreted glycoproteins Which direct cell proliferation, polarity, and fate decisions during embryonic development. (jneurosci.org)
  • Disease specific iPS cell lines maintain a morphology similar to hES cells when grown in co-culture with mouse embryonic feeder fibroblasts (MEFs). (nih.gov)
  • A method of collecting embryonic-like stem cells from a placenta which has been treated to remove residual cord blood by perfusing the drained placenta with an anticoagulant solution to flush out residual cells, collecting the residual cells and perfusion liquid from the drained placenta, and separating. (google.es)
  • Stem cells within teeth originating from the embryonic neural crest have attracted increasing attention in clinical and scientific research because they are easy to obtain and have superb stemness. (stemsave.com)
  • However, the curative rate of this treatment, especially for cord blood (CB) HSCT, must be improved because of the low homing efficiency of haematopoietic stem and progenitor cells (HSPCs), delayed engraftment and the occurrence of graft versus host diseases. (biomedcentral.com)
  • Results Multicolor cell sorting and CFU-F assays showed that mesenchymal stem cells were ~90-fold enriched in the MSCA-1 + CD56 − fraction and ~180-fold in the MSCA-1 + CD56 + fraction. (haematologica.org)
  • Transcriptional analysis unveiled the down-regulation of global regulator sarA and sarA mediated virulence genes upon myrtenol treatment, which is well correlated with results of phenotypic assays. (frontiersin.org)
  • These results indicate that Sox17 suppresses cyclin D1 expression and cell proliferation by directly antagonizing β-catenin, whose activity in OPCs is stimulated not only by Wnt3a, but also by PDGF. (jneurosci.org)
  • The results of the bioluminescence adenosine triphosphate assay and CFUs of the groups were analyzed by means of analysis of variance and the Duncan test. (ovid.com)
  • We investigated the immunomodulatory effects of DFSCs on CD4 + T helper cell responses of asthmatic patients and compared the results with those obtained with asthmatic subjects on immunotherapy and with healthy individuals. (stemsave.com)
  • Results of the colony-forming unit assay showed an increase in the number of different colonies following treatment with olive leaf phytochemicals. (alliedacademies.org)
  • Moreover, TrkA overexpression inhibited long-term proliferation of both the neuronal SK-N-MC cells and the non-neuronal U2OS cells, suggesting a potential role of TrkA as a tumor suppressor. (biomedsearch.com)
  • Cell aggregation induces phosphorylation of PECAM-1 and Pyk2 and promotes tumor cell anchorage-independent growth. (nih.gov)
  • We investigated the mechanisms of anoikis resistance of tumor cells. (nih.gov)
  • We demonstrated a correlation between tumor cell aggregation in suspension and cell growth in soft agar. (nih.gov)
  • The data demonstrated that Pyk2 and PECAM-1 were critical mediators of both anchorage-independent growth and anoikis resistance in tumor cells. (nih.gov)
  • Many mutated genes contribute to tumorigenesis by conferring on the cells enhanced self-renewal and proliferation capacities, 6 which represent a growth advantage over normal cells. (nature.com)
  • Together these transcription factors form a key activator complex that regulates the expression of genes involved in leukemogenesis and its overexpression is significant related to adverse prognosis in AML. (cnio.es)
  • Treatment of cells with HXR9, a specific peptide inhibitor of HOXA9 and PBX3 interaction, led to a selective decrease in the proliferation of hHP expressing NUP98-HOXA9, confirming the relevance of these target genes to its oncogenic mechanism. (cnio.es)
  • Microarray analysis in oligodendrocyte progenitor cells (OPCs) after Sox17 attenuation revealed upregulated genes associated with cell cycle control and activation of the Wingless and integration site (Wnt)/β-catenin pathway. (jneurosci.org)
  • A two-way ANOVA was applied to the array data to identify genes differentially expressed in the two cell types after TLR2/1L treatment ( 6 ). (sciencemag.org)
  • Bacterial settling assays showed that EAEC strains harboring F-pili genes ( traA ) were capable of forming bacterial aggregates only in the presence of EACF. (springer.com)
  • In order to broaden the array of tools for therapeutic application, we iso- lated a new population of cells from adult human testis termed gonadal stem cells (GSCs). (slideshare.net)
  • Both tofacitinib and oxozeaenol significantly improved GAG content in cell pellets from these adult donors. (cellmedicine.com)
  • 1.3 The cells may include adult, progenitor or stem cells from any species. (astm.org)
  • The first reason they do this is because the UCB unit must be big enough to transplant an adult. (parentsguidecordblood.org)
  • Effects of low level maternal inhalation exposure to benzene (1076433) (Bz) on erythrocytic and granulocytic colony forming cells of fetal, neonatal, and young adult offspring were studied. (cdc.gov)
  • A multi-center analysis of adverse events among two thousand, three hundred and seventy two adult patients undergoing adult autologous stem cell therapy for orthopaedic conditions. (regenexx.com)
  • However, while this treatment modality has increased cord blood usage in adult patients, many patients remain ineligible, as the total cell dose of two CBU may still be insufficient for many adult patients. (biomedcentral.com)
  • Inflammatory periodontal diseases represent a serious dental and general medical problem due to the high prevalence among the adult population, the presence of clinical forms leading to the destruction of the dentition and tooth loss, insufficient treatment effectiveness and the frequency of relapse, including in connection with the formation of biofilms. (bvsalud.org)
  • The presence of eGFP had no effect on cell surface marker expression, and mMSCs derived from both bone marrow and adipose tissue had similar surface marker profiles. (biomedcentral.com)