Mesenchymal Stromal Cells: Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.Mesenchymal Stem Cell Transplantation: Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).Traumatology: The medical specialty which deals with WOUNDS and INJURIES as well as resulting disability and disorders from physical traumas.Tissue Engineering: Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.Tissue Scaffolds: Cell growth support structures composed of BIOCOMPATIBLE MATERIALS. They are specially designed solid support matrices for cell attachment in TISSUE ENGINEERING and GUIDED TISSUE REGENERATION uses.Hypophosphatasia: A genetic metabolic disorder resulting from serum and bone alkaline phosphatase deficiency leading to hypercalcemia, ethanolamine phosphatemia, and ethanolamine phosphaturia. Clinical manifestations include severe skeletal defects resembling vitamin D-resistant rickets, failure of the calvarium to calcify, dyspnea, cyanosis, vomiting, constipation, renal calcinosis, failure to thrive, disorders of movement, beading of the costochondral junction, and rachitic bone changes. (From Dorland, 27th ed)Alkaline Phosphatase: An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.Osteogenesis: The process of bone formation. Histogenesis of bone including ossification.Adipose Tissue: Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.BooksPublishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.MEDLINE: The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).Disposable Equipment: Apparatus, devices, or supplies intended for one-time or temporary use.Bioreactors: Tools or devices for generating products using the synthetic or chemical conversion capacity of a biological system. They can be classical fermentors, cell culture perfusion systems, or enzyme bioreactors. For production of proteins or enzymes, recombinant microorganisms such as bacteria, mammalian cells, or insect or plant cells are usually chosen.Equipment Reuse: Further or repeated use of equipment, instruments, devices, or materials. It includes additional use regardless of the original intent of the producer as to disposability or durability. It does not include the repeated use of fluids or solutions.Stainless Steel: Stainless steel. A steel containing Ni, Cr, or both. It does not tarnish on exposure and is used in corrosive environments. (Grant & Hack's Chemical Dictionary, 5th ed)Laryngoscopes: Endoscopes for examining the interior of the larynx.Access to Information: Individual's rights to obtain and use information collected or generated by others.Stem Cell Research: Experimentation on STEM CELLS and on the use of stem cells.Journal Impact Factor: A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.Peer Review, Research: The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.Menu PlanningRestaurantsFood Labeling: Use of written, printed, or graphic materials upon or accompanying a food or its container or wrapper. The concept includes ingredients, NUTRITIONAL VALUE, directions, warnings, and other relevant information.Health Policy: Decisions, usually developed by government policymakers, for determining present and future objectives pertaining to the health care system.Portion Size: The amount of a particular food one chooses to eat at a single meal. It is different from SERVING SIZE, which is a reference amount of food as defined by an authoritative source, such as the Food Guide Pyramid devised by the UNITED STATES DEPARTMENT OF AGRICULTURE.Hair Cells, Auditory: Sensory cells in the organ of Corti, characterized by their apical stereocilia (hair-like projections). The inner and outer hair cells, as defined by their proximity to the core of spongy bone (the modiolus), change morphologically along the COCHLEA. Towards the cochlear apex, the length of hair cell bodies and their apical STEREOCILIA increase, allowing differential responses to various frequencies of sound.Organ of Corti: The spiral EPITHELIUM containing sensory AUDITORY HAIR CELLS and supporting cells in the cochlea. Organ of Corti, situated on the BASILAR MEMBRANE and overlaid by a gelatinous TECTORIAL MEMBRANE, converts sound-induced mechanical waves to neural impulses to the brain.Hair Cells, Auditory, Inner: Auditory sensory cells of organ of Corti, usually placed in one row medially to the core of spongy bone (the modiolus). Inner hair cells are in fewer numbers than the OUTER AUDITORY HAIR CELLS, and their STEREOCILIA are approximately twice as thick as those of the outer hair cells.Cochlea: The part of the inner ear (LABYRINTH) that is concerned with hearing. It forms the anterior part of the labyrinth, as a snail-like structure that is situated almost horizontally anterior to the VESTIBULAR LABYRINTH.Stem Cell Transplantation: The transfer of STEM CELLS from one individual to another within the same species (TRANSPLANTATION, HOMOLOGOUS) or between species (XENOTRANSPLANTATION), or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). The source and location of the stem cells determines their potency or pluripotency to differentiate into various cell types.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Regeneration: The physiological renewal, repair, or replacement of tissue.Nerve Regeneration: Renewal or physiological repair of damaged nerve tissue.Liver Regeneration: Repair or renewal of hepatic tissue.Voltage-Gated Sodium Channel beta-2 Subunit: A voltage-gated sodium channel beta subunit that binds covalently to voltage-gated alpha subunits.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Hematopoietic Stem Cells: Progenitor cells from which all blood cells derive.Bone Marrow Cells: Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Embryo Research: Experimentation on, or using the organs or tissues from, a human or other mammalian conceptus during the prenatal stage of development that is characterized by rapid morphological changes and the differentiation of basic structures. In humans, this includes the period from the time of fertilization to the end of the eighth week after fertilization.Embryonic Stem Cells: Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.Transplantation, Autologous: Transplantation of an individual's own tissue from one site to another site.Bone Marrow Transplantation: The transference of BONE MARROW from one human or animal to another for a variety of purposes including HEMATOPOIETIC STEM CELL TRANSPLANTATION or MESENCHYMAL STEM CELL TRANSPLANTATION.Microglia: The third type of glial cell, along with astrocytes and oligodendrocytes (which together form the macroglia). Microglia vary in appearance depending on developmental stage, functional state, and anatomical location; subtype terms include ramified, perivascular, ameboid, resting, and activated. Microglia clearly are capable of phagocytosis and play an important role in a wide spectrum of neuropathologies. They have also been suggested to act in several other roles including in secretion (e.g., of cytokines and neural growth factors), in immunological processing (e.g., antigen presentation), and in central nervous system development and remodeling.Brain Injuries: Acute and chronic (see also BRAIN INJURIES, CHRONIC) injuries to the brain, including the cerebral hemispheres, CEREBELLUM, and BRAIN STEM. Clinical manifestations depend on the nature of injury. Diffuse trauma to the brain is frequently associated with DIFFUSE AXONAL INJURY or COMA, POST-TRAUMATIC. Localized injuries may be associated with NEUROBEHAVIORAL MANIFESTATIONS; HEMIPARESIS, or other focal neurologic deficits.Platelet-Rich Plasma: A preparation consisting of PLATELETS concentrated in a limited volume of PLASMA. This is used in various surgical tissue regeneration procedures where the GROWTH FACTORS in the platelets enhance wound healing and regeneration.Platelet Aggregation: The attachment of PLATELETS to one another. This clumping together can be induced by a number of agents (e.g., THROMBIN; COLLAGEN) and is part of the mechanism leading to the formation of a THROMBUS.Fasciitis, Plantar: Inflammation of the thick tissue on the bottom of the foot (plantar fascia) causing HEEL pain. The plantar fascia (also called plantar aponeurosis) are bands of fibrous tissue extending from the calcaneal tuberosity to the TOES. The etiology of plantar fasciitis remains controversial but is likely to involve a biomechanical imbalance. Though often presenting along with HEEL SPUR, they do not appear to be causally related.Blood Platelets: Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.Bone Regeneration: Renewal or repair of lost bone tissue. It excludes BONY CALLUS formed after BONE FRACTURES but not yet replaced by hard bone.

*Food and Drug Administration

These include blood and blood products, vaccines, allergenics, cell and tissue-based products, and gene therapy products. New ... Districts are based roughly on the geographic divisions of the federal court system. Each district comprises a main district ... The most rigorous requirements apply to new molecular entities: drugs that are not based on existing medications. New drugs ... This guidance distinguishes the apps subjected to regulation based on the marketing claims of the apps. Incorporation of the ...

*Magnetic resonance therapy

Based on first qualitative evidence that magnetic resonance might regenerate cartilage tissue, a number of further studies were ... It aims to activate reparative processes in specific cells and tissues. There is no major clinical trial supporting the ... Magnetic resonance therapy is a proposed alternative treatment method based on the physical principle of magnetic resonance. ... Moreover, the therapy is applied for the prevention and treatment of osteoporosis as well as disorder of metabolisms in the ...

*Spinal cord injury

Kabu, S.; Gao, Y.; Kwon, B.K.; Labhasetwar, V. (2015). "Drug delivery, cell-based therapies, and tissue engineering approaches ... Types of cells being researched for use in SCI include embryonic stem cells, neural stem cells, mesenchymal stem cells, ... olfactory ensheathing cells, Schwann cells, activated macrophages, and induced pluripotent stem cells. Hundreds of stem cell ... "Cell Therapy Augments Functional Recovery Subsequent to Spinal Cord Injury under Experimental Conditions". Stem Cells ...

*Spinal cord injury research

... cell-based therapies, and tissue engineering approaches for spinal cord injury". Journal of Controlled Release. 219: 141-54. ... Stem cells are cells that can differentiate to become different types of cells. The hope is that stem cells transplanted into ... Types of cells being researched for use in SCI include embryonic stem cells, neural stem cells, mesenchymal stem cells, ... "Hydrogels and Cell Based Therapies in Spinal Cord Injury Regeneration". Stem Cells International. 2015: 948040. doi:10.1155/ ...

*Regeneration in humans

Kabu, S.; Gao, Y.; Kwon, B.K.; Labhasetwar, V. (2015). "Drug delivery, cell-based therapies, and tissue engineering approaches ... In humans with non-injured tissues, the tissue is naturally regenerated over time; by default these tissues have new cells ... 2014). "Humans iPS cell-engineered cardiac tissue sheets with cardiomyocytes and vascular cells for cardiac regeneration". ... It has been shown that bone marrow-derived cells could be the source of progenitor cells of multiple cell lineages, and a 2004 ...

*Expert Opinion on Biological Therapy

... and cell- and tissue-based therapies. The journal is published by Taylor & Francis and the editor-in-chief is Michael Morse ( ... Expert Opinion on Biological Therapy is a monthly peer-reviewed medical journal covering research on all aspects of biological ... "Expert Opinion on Biological Therapy". 2016 Journal Citation Reports. Web of Science (Science ed.). Thomson Reuters. 2015. ... therapy, including gene therapy and gene transfer technologies, therapeutic peptides and proteins, vaccines and antibodies, ...

*Biopharmaceutical

... "based on genes, cells, or tissue engineering", including gene therapy medicines, somatic-cell therapy medicines, tissue- ... somatic cells, gene therapies, tissues, recombinant therapeutic protein, and living cells used in cell therapy. Biologics can ... mammalian cell lines (see cell culture) and plant cell cultures (see plant tissue culture) and moss plants in bioreactors of ... Important biologics include: Whole blood and other blood components Organs and tissue transplants Stem cell therapy Antibodies ...

*ATMP (disambiguation)

... a classification for medicinal products that are based on genes, cells or tissues. ... an album by George Harrison Advanced Therapy Medicinal Product, ...

*McGowan Institute for Regenerative Medicine

The McGowan Institute works to address tissue and organ insufficiency through tissue engineering, cell-based therapies, and ... McGowan Institute currently works on tissue and organ insufficiency through tissue engineering, cell-based therapies, and ... research of the faculty affiliated with the McGowan Center with research related to tissue engineering and cell-based therapies ... McGowan Institute is: Developing and delivering therapies that reestablish tissue and organ function; Creating and sharing ...

*Institute for Bioengineering of Catalonia

... the biophysical mechanisms underlying migration at the level of cells and tissues; biomimetic systems for cell-based assays; ... Bioengineering for Regenerative Therapies: Molecular dynamics at the interface of cells and biomaterials; ... About us section of IBEC website Severo Ochoa Centre of Excellence MedicalXpress: Cancer cells hijack healthy cells Health ... cell migration, stem cells, artificial olfaction and microbial biotechnology. The institute was created by the Government of ...

*Electroporation

... and cell-based therapy. The process of introducing foreign DNA into eukaryotic cells is known as transfection. Electroporation ... When the electricity applied to the tissue is above the electric field threshold of the target tissue, the cells become ... Electrofusion of mesenchymal stem cells and islet cells for diabetes therapy: A rat model. PLoS ONE 8, e64499 (2013). McCluskey ... or other molecule that is not normally permeable to the cell membrane) into the cell. Not all tissue has the same electric ...

*3H Biomedical

The company develops, produces and markets cell-based tools for life science, tissue engineering, drug discovery and cosmetics ... The company's research and development focus lies in the fields of cell immune therapy and adult stem cell therapy. 3H ... The company started out marketing and selling primary cells, and has since extended its portfolio with cell chips, RNA, DNA, ... 3H Biomedical is a biotechnology company based in Uppsala, Sweden, where it operates out of Uppsala Science Park. ...

*W. Mark Saltzman

... and tissue engineering. He studies how to create safer and more effective medical and surgical therapy based on tissue ... Saltzman built scaffolds that could be seeded with cells to sculpt new replacement tissues. He also created drug-impregnated ... Nanoparticle-based therapy in an in vivo miR-155-dependent mouse model of lymphoma, Proceedings of the National Academy of ... 1990) Books Drug Delivery: Engineering Principles for Drug Therapy, 2001, Published by Oxford University Press. Tissue ...

*Photoimmunotherapy

... which allows the selective destruction of cancer cells without any damage to normal tissues. It is a light-based cancer therapy ... Based on the current hypothesis, cell death induced by PIT was caused by rapid expansion of local water upon the formation of ... "Immunological responses triggered by photothermal therapy with carbon nanotubes in combination with anti-CTLA-4 therapy to ... Conventional photodynamic therapy (PDT) uses a non-specific photosensitizer which can be activated by a non-ionizing light to ...

*MicroRNA

MiRNA-based therapies are under investigation.. Estimates of the average number of unique messenger RNAs that are targets for ... miRNA research revealed different sets of miRNAs expressed in different cell types and tissues and multiple roles for miRNAs in ... and cell cycle in mice lacking miRNA-1-2". Cell. 129 (2): 303-17. doi:10.1016/j.cell.2007.03.030. PMID 17397913. Thum T, ... B-cell migration/adhesion, cell-cell interactions in immune niches and the production and class-switching of immunoglobulins. ...

*Cell encapsulation

"Microencapsulated stem cells for tissue repairing: implications in cell-based myocardial therapy". Regen Med. 4 (5): 733-45. ... An emerging approach to answer the problems related to ischemic tissue repair is through the use of stem cell-based therapy. ... research is the use of stem cells such as mesenchymal stem cells for long term cell microencapsulation and cell therapy ... xenogeneic cells). The use of autologous cells in microencapsulation therapy is limited by the availability of these cells and ...

*Cell bank

"Cryopreservation and Cell Banking for Autologous Mesenchymal Stem Cell-Based Therapies". Cell & Tissue Transplantation & ... thus preserving the cell line, which is especially useful when the cell line has a limited number of cell divisions. Cells can ... They often contain expansive amounts of base cell material that can be utilized for various projects. Cell banks can be used to ... Utilizing cell banks also reduces the cost of cell culture processes, providing a cost-efficient alternative to keeping cells ...

*Cytori Therapeutics

is a late stage cell therapy company developing autologous cell therapies from adipose tissue to treat a variety of medical ... Cytori Therapeutics, Inc., of the United States, is a pharmaceutical company, based in San Diego. The company develops and ... This cell therapy product is prepared from a patient's own adipose (fat) tissue using the company's fully automated Celution® ... The system formulates a cell therapy product that is available for delivery to the patient within 1-2 hours of tissue ...

*Basal-cell carcinoma

Diagnosis is often based on skin examination and confirmed by tissue biopsy. It is not clear if sunscreen affects the risk of ... Photodynamic therapy (PDT) is a new modality for treatment of basal-cell carcinoma, which is administrated by application of ... Basal cell carcinoma can be divided into three groups, based on the growth patterns. Superficial basal cell carcinoma, formerly ... Cicatricial basal cell carcinoma (also known as "morpheaform basal cell carcinoma," and "morphoeic basal cell carcinoma") is an ...

*Induced stem cells

"Mesenchymal Stem Cell Exosomes: The Future MSC-Based Therapy?". Mesenchymal Stem Cell Therapy. pp. 39-61. doi:10.1007/978-1- ... Pimanda describe the generation of tissue-regenerative multipotent stem cells (iMS cells) by treating mature bone and fat cells ... Induced Vascular Progenitor Cells (iVPCs) are useful for cell-based therapy designed to stimulate coronary collateral growth. ... may provide such cells. Cell-based in vivo therapies may provide a transformative approach to augment vascular and muscle ...

*Neural Regeneration Research

Topics covered include neural stem cells, nerve tissue engineering, gene therapy, neurodegenerative diseases, and traditional ... evidence-based case reports, commentaries/review, and case reports. The journal is abstracted and indexed in BIOSIS Previews, ... Neural Regeneration Research is a peer-reviewed scientific journal covering research on neuroregeneration and stem cells. ...

*Squamous cell skin cancer

It begins from squamous cells found within the skin. Diagnosis is often based on skin examination and confirmed by tissue ... Radiation therapy is a primary treatment option for patients in whom surgery is not feasible and is an adjuvant therapy for ... Information on Squamous Cell Carcinoma from The Skin Cancer Foundation DermNet NZ: Squamous cell carcinoma Squamous cell ... Squamous-cell skin cancer is more likely to spread to distant areas than basal cell cancer. The greatest risk factor is high ...

*Glassy cell carcinoma of the cervix

The diagnosis is based on tissue examination, e.g. biopsy. Under the microscope, glassy cell carcinoma tumours are composed of ... Advanced tumours are treated with surgery (radical hysterectomy and bilateral salpingo-opherectomy), radiation therapy and ... Glassy cell carcinoma of the cervix, also glassy cell carcinoma, is a rare aggressive malignant tumour of the uterine cervix. ... "Glassy cell carcinoma of the uterine cervix a rare histology. Report of three cases with a review of the literature". Indian J ...

*Stephen J. Lippard

Placon Therapeutics is developing platinum-based cancer therapies. These include BTP-114, the first clinical candidate to use ... with the goal of targeting cancerous tissue and leaving healthy cells alone. Its proprietary drug candidates included BTP-114, ... He has made major contributions in a number of areas, including the development of platinum-based anticancer drugs such as the ... Rosenberg, B.; Van Camp, L.; Krigas, T. (1965). "Inhibition of Cell Division in Escherichia coli by Electrolysis Products from ...

*Muse cell

... they could provide an ideal source of pluripotent stem cells for regenerative medicine and cell-based therapy. Currently, Life ... "Muse Cells Provide the Pluripotency of Mesenchymal Stem Cells: Direct Contribution of Muse Cells to Tissue Regeneration". Cell ... Other than mesenchymal tissues, Muse cells locate in connective tissue of every organ and in the peripheral blood. Muse cells ... Muse cells act as tissue repairing cells in vivo. When systemically administrated, naive Muse cells (without cytokine treatment ...

*Hemoencephalography

Photoelectric cells in a spectrophotometer device worn on the forehead measure the amount of each wavelength of light reflected ... Neurofeedback, a specific form of biofeedback, is based on the idea that human beings can consciously alter their brain ... Mize, W. (2004). Hemoencephalography-a new therapy for attention deficit hyperactivity disorder (ADHD): case report. Journal of ... which are responsible for the transport and transference of oxygen to tissue throughout the body, must increase the amount of ...
TY - JOUR. T1 - Hypoxia-preconditioned adipose tissue-derived mesenchymal stem cell increase the survival and gene expression of engineered neural stem cells in a spinal cord injury model. AU - Oh, Jin Soo. AU - Ha, Yoon. AU - An, Sung Su. AU - Khan, Momin. AU - Pennant, William A.. AU - Kim, Hyo Jin. AU - Yoon, Do Heum. AU - Lee, Minhyung. AU - Kim, Keung Nyun. PY - 2010/3/26. Y1 - 2010/3/26. N2 - Hypoxic preconditioning (HP) is a novel strategy to make stem cells resistant to the ischemic environment they encounter after transplantation into injured tissue; this strategy improves survival of both the transplanted cells and the host cells at the injury site. Using both in vitro and in vivo injury models, we confirmed that HP-treated adipose tissue-derived mesenchymal stem cells (HP-AT-MSCs) increased cell survival and enhanced the ...
ATCC ® Normal Human Adipose-Derived Mesenchymal Stem Cells, when grown in Mesenchymal Stem Cell Basal Media supplemented with Mesenchymal Stem Cell Growth Kit Low serum components, provide an ideal cell system to propagate mesenchymal stem cells in low serum (2% FBS) conditions. When maintained under optimal growth conditions, ATCC Normal Human Adipose-Derived Mesenchymal Stem Cells have been shown to be multipotent, capable of differentiating down the adipogenic, osteogenic, and chondrogenic lineages. The cells are cryopreserved at the second passage to ensure the highest viability and plating efficiency. ATCC ® Primary Cell Solutions™ cells, media, supplements and reagents are quality tested together to guarantee optimum performance and reliability.
Background: Developing efficient methods to isolate and identify human adipose-derived mesenchymal stem cells (hADSCs) remains to be one of the major challenges in tissue engineering.. Methods: We demonstrate here a method by isolating hADSCs from abdominal subcutaneous adipose tissue harvested during caesarian section. The hADSCs were isolated from human adipose tissue by collagenase digestion and adherence to flasks.. Results: The yield reached around 1 x 10(6) hADSCs per gram adipose tissue. The following comprehensive identification and characterization illustrated pronounced features of mesenchymal stem cells (MSCs). The fibroblast-like hADSCs exhibited typical ultrastructure details for vigorous cell activities. Karyotype mapping showed normal human chromosome. With unique immunophenotypes they were positive for CD29, CD44, CD73, CD105 and CD166, but negative for CD31, CD34, CD45 and ...
Background: Increasing evidences indicated that adipose-derived mesenchymal stem cells (ADMSCs) can stay survive, then gradually proliferate and differentiate into myocardial cells after transplanted into damaged areas and improve function of heart.M
Background: Cell therapy is a potential therapeutic approach for neurodegenerative disorders. Human umbilical cord bloodderived mesenchymal stem cells (hUCB-MSCs) are an appropriate source of stem cells for use in various cell-based therapies. Objectives: In this study, we examined a real-time PCR approach for neural differentiation of hUCB-MSCs in vitro. Materials and Methods: MSCs were cultured in DMEM medium supplemented with 10% FBS in a humidified incubator equilibration at 5% CO2 and 37°C. For the neural differentiation of MSCs, the DMEM was removed and replaced with pre-induction media (retinoic acid [RA], basic fibroblast growth factor [bFGF], and epidermal growth factor [EGF]) and basal medium for two days. They were then cultured in nerve growth factor (NGF), 3-isobutyl-1-methylxanthine (IBMX), ascorbic acid (AA), and basal medium for six days. We also monitored the expression of markers for neural ...
This study is aiming to investigate the efficacy of autologous mesenchymal stem cell (MSC) transplantation in 25 randomized patients with liver cirrhosis autologous mesenchymal stem cell will be derived from patients bone marrows and will be infused via peripheral vein. Liver biopsies will be performed in every patient in the beginning and at 6th month ...
This study will explore safety and efficacy of allogeneic mesenchymal stem cells transplantation (MSCT) to treat patients with diagnosis of primary Sjögrens Syndrome (pSS) who have been resistant to multiple standard treatments. The underlying hypothesis is that the pSS condition is caused by an abnormal immune homeostasis that can be restored by MSCT ...
In this study, after 21 days of ceasing CCl 4 , both placebo-treated and untreated cirrhotic mice showed reduced liver damage as indicated by a decrease of AST/ALT index, recovery of histology, and reduction of fibrosis-related gene expression. Presumably, the liver of these mice were capable of self-regeneration. The cause of the self-regeneration has been explained by the ceasing of CCl 4 administration during the 21 days of treatment. Other studies have shown similar results to our study Carvalho et al., 2008 Kuo et al., 2008 .. However, our study uniquely demonstrates that stem cell treatment in cirrhotic mice was better for restoring liver function and histology (significantly better than placebo treatment). The effects of stem cell treatment in our mouse model of liver cirrhosis may be attributed to stem cell immunomodulation, stem cell homing and differentiation, and secreted cytokines Berardis et al., 2015 .. In terms of stem ...
Bone marrow mesenchymal stem cells (MSCs) are one of the potential tools for treatment of the spinal cord injury; however, the survival and differentiation of MSCs in an injured spinal cord still need to be improved. In the present study, we investigated whether Governor Vessel electro-acupuncture (EA) could efficiently promote bone marrow mesenchymal stem cells (MSCs) survival and differentiation, axonal regeneration and finally, functional recovery in the transected spinal cord. The spinal cords of adult Sprague-Dawley (SD) rats were completely transected at T10, five experimental groups were performed: 1. sham operated control (Sham-control); 2. operated control (Op-control); 3. electro-acupuncture treatment (EA); 4. MSCs transplantation (MSCs); and 5. MSCs transplantation combined with electro-acupuncture (MSCs+EA). After 2-8 weeks of MSCs transplantation plus EA treatment, we found that the neurotrophin-3 (NT-3), cAMP level, the differentiation of MSCs, the 5-HT ...
ATCC ® Normal Human Umbilical Cord-Derived Mesenchymal Stem Cells, when grown in Mesenchymal Stem Cell Basal Media supplemented with Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low serum components, provide an ideal cell system to propagate mesenchymal stem cells in low serum (2% FBS) conditions. When maintained under optimal growth conditions, ATCC Normal Human Umbilical Cord-Derived Mesenchymal Stem Cells have been shown to be multipotent, capable of differentiating down the adipogenic, osteogenic, and chondrogenic lineages. The cells are cryopreserved at the second passage to ensure the highest viability and plating efficiency. ATCC® Primary Cell Solutions™ cells, media, supplements and reagents are quality tested together to guarantee optimum performance and reliability.
Proteasome Activation Enhances Stemness and Lifespan of Human Mesenchymal Stem Cells By employing both Whartons-Jelly- and Adipose-derived human adult mesenchymal stem cells, investigators revealed a significant age-related decline in proteasome content and peptidase activities, accompanied by alterations of proteasomal complexes. [Free Radic Biol Med] Abstract Human Umbilical Cord Blood-Stem Cells Direct Macrophage Polarization and Block Inflammasome Activation to Alleviate Rheumatoid Arthritis Researchers investigated the therapeutic potential of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) as a new therapeutic strategy for patients with rheumatoid arthritis and to explore the mechanisms underlying hUCB-MSC-mediated immunomodulation. [Cell Death Dis] Full Article Therapeutic Effects of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Acute Lung Injury Mice Scientists found ...
TY - JOUR. T1 - Survivin Is Required for Mouse and Human Bone Marrow Mesenchymal Stromal Cell Function. AU - Singh, Pratibha. AU - Fukuda, Seiji. AU - Liu, Liqiong. AU - Chitteti, Brahmananda Reddy. AU - Pelus, Louis. PY - 2018/1/1. Y1 - 2018/1/1. N2 - Although mesenchymal stromal cells (MSCs) have significant potential in cell-based therapies, little is known about the factors that regulate their functions. While exploring regulatory molecules potentially involved in MSC activities, we found that the endogenous multifunctional factor Survivin is essential for MSC survival, expansion, lineage commitment, and migration. Pharmacological or genetic blockade of Survivin expression in mouse and human bone marrow MSC enhances caspase 3 and 7 expression and reduces proliferation resulting in fewer MSC and clonogenic colony-forming unit-fibroblasts (CFU-F), whereas ectopic Survivin overexpression in MSC results in their expansion. Survivin is also ...
Cytotoxic T-lymphocyte-associated protein 4- (CTLA4-) modified human bone marrow-derived mesenchymal stem cells (hBMMSCs) might be promising seed cells for bone tissue engineering. However, the underlying mechanism is not clear. In the present study, we investigated whether CTLA4-modified hBMMSCs are involved in the migration of allogeneic hBMMSCs (allo-hBMMSCs) by maintaining POSTN secretion. hBMMSCs were isolated from different groups, named hBMMSCs and allo-hBMMSCs. hBMMSCs that were infected with the negative control (NC), empty adenovirus- or recombinant adenovirus-expressing CTLA4, POSTN, or CTLA4 plus the shRNA of POSTN were named NC hBMMSCs, CTLA4-modified hBMMSCs, POSTN-modified hBMMSCs, or CTLA4+shPOSTN-modified hBMMSCs, respectively. They were then cocultured with PBMCs in a 1 : 5 ratio with 2.5 |i|μ|/i|g/mL phytohemagglutinin (PHA). The coculture supernatant was collected to treat allo-hBMMSCs with anti-integrin ...
OBJECTIVE To explore the response of rat bone marrow mesenchymal stem cells (MSCs and calvarial osteoblasts to mechanical strain and the consequent changes of cytoskeleton F-actin. METHODS Bone marrow MSCs and calvarial osteoblasts were isolated from SD rats and cultured in vitro. Mechanical stretch was performed on passage 3 cells at 2 000 microepsilon for 0, 2, 6 and 12 hours using four-point bending system. The response of cells and the distribution of F-actin were observed using fluorescent staining under laser scanning confocal microscope and the morphological parameters were quantified using image analysis software Laserpix. RESULTS Under mechanical stretch, the fluorescent staining decreased obviously at both MSCs and osteoblasts, and F-actin filaments were rearranged and became tenuous, thinner, and abnormally distributed. The outline of nucleus became unclear and apoptotic changes were observed at some of both cells. ...
Ge, Y.; Zhang, Y.; Tang, Q.; Gao, J.; Yang, H.; Gao, Z.; Zhao, R.Chunhua., 2019: Mechanisms of the Immunomodulation Effects of Bone Marrow-Derived Mesenchymal Stem Cells on Facial Nerve Injury in Sprague-Dawley Rats
We refer to two articles by Duijvestein et al and Ciccocioppo et al1 2 in which the authors suggest that administration of autologous bone marrow-derived mesenchymal stem cells (MSCs) is safe and feasible in the treatment of refractory Crohns disease (CD). However, to date there are few data about allogeneic MSC transplantation (MSCT) for patients with inflammatory bowel disease (IBD).. We wish to report our experience of allogeneic MSCT in seven patients with IBD.. Infused allogeneic MSCs were obtained from the bone marrow or umbilical cord. Bone marrow was aspirated from healthy relatives of three patients. Umbilical cords were obtained from local maternity hospitals after normal deliveries. The isolated MSCs were given by intravenous infusions … ...
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Fang S, et al. Umbilical Cord-Derived Mesenchymal Stem Cell-Derived Exosomal MicroRNAs Suppress Myofibroblast Differentiation by Inhibiting the Transforming Growth Factor-b/SMAD2 Pathway During Wound Healing.(IF=5.902). ...
Purpose: : To observe the differentiation potential of SD rat bone marrow mesenchymal stem cells (MSCs) which were subretinally transplanted into laser-injured retina in vivo without differentiation inducements in vitro. Methods: : SD rat MSCs were cultivated in vitro to passage 3 and were injected into the sub-retina space of laser-injured rat retina with Bromo-Deoxyuridine (BrdU) labeling to trace the origin of the repopulating cells. After 4 weeks, eyeballs were enucleated for continuous paraffin slides. The expressions of Nestin, NeuN, GFAP, Vimentin, Rhodopsin were detected by immunofluorescence. Results: : BrdU-positive MSCs were present in mutiple locations, including photoreceptor layer, the bipolar layers and the ganglion cell layer. These cells expressed Nestin, NeuN, GFAP, Vimentin, and Rhodopsin. Conclusions: : For SD rats, transplanted MSCs in injured retina can be integrated into the host retina and partially differentiate into ...
Rheumatoid arthritis is a chronic systemic disease, which is characterized by chronic inflammation in the synovial tissue. Rheumatoid arthritis will eventually result in the destruction of cartilage, bone and ligaments and joint deformity. The underlying hypothesis is that umbilical cord-derived mesenchymal stem cell (UC-MSCs) has anti-inflammatory effects and thus potentially alleviates the progression of rheumatoid arthritis. The study is to explore the safety and efficacy of UC-MSCs transplantation in treatment of rheumatoid arthritis ...
Intranasal Administration of Mesenchymoangioblast-Derived Mesenchymal Stem Cells Abrogates Airway Fibrosis and Airway Hyperresponsiveness Associated with Chronic Allergic Airways Disease Researchers assessed the in vivo efficacy of induced pluripotent stem cell and mesenchymoangioblast-derived mesenchymal stem cells (MCA-MSCs) on airway remodeling in a murine model of chronic allergic airways disease/asthma. Apart from epithelial thickness, all other parameters measured were significantly, although not totally, decreased by intravenous delivery of MCA-MSCs to ovalbumin-injured mice. [FASEB J] Abstract Identification and Characterization of a Rich Population of CD34+ Mesenchymal Stem/Stromal Cells in Human Parotid, Sublingual and Submandibular Glands Very little is known about the phenotype, distribution and molecular nature of mesenchymal stem/stromal cells (MSCs) in freshly isolated human salivary glands (SGs) as most ...
Toxic shock syndrome (TSS) is caused by an overwhelming host-mediated response to bacterial superantigens produced mainly by Staphylococcus aureus and Streptococcus pyogenes. TSS is characterized by aberrant activation of T cells and excessive release of pro-inflammatory cytokines ultimately resulting in capillary leak, septic shock, multiple organ dysfunction and high mortality rates. No therapeutic or vaccine has been approved by the U.S. Food and Drug Administration for TSS, and novel therapeutic strategies to improve clinical outcome are needed. Mesenchymal stromal (stem) cells (MSCs) are stromal cells capable of self-renewal and differentiation. Moreover, MSCs have immunomodulatory properties, including profound effects on activities of T cells and macrophages in specific contexts. Based on the critical role of host-derived immune mediators in TSS, we hypothesized that MSCs could modulate the host-derived ...
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Additional evidence comes from a group of researchers and clinicians from the United Kingdom who treated MS patients in the study, "Autologous Mesenchymal Stem Cells for the Treatment of Secondary Progressive Multiple Sclerosis: An Open-Label Phase 2a Proof-of-Concept Study.". Research findings show that intravenous administration of autologous mesenchymal stem cells to patients with secondary progressive multiple sclerosis is feasible and safe and suggests structural, functional, and physiological improvement in patients after receiving treatment with autologous mesenchymal stem cells which is consistent with remyelination. In addition, patients experienced enhanced visual acuity.. Dr. Andre Lallande, Medical Director of StemGenex Medical Group and Principal Investigator of the observational study "Outcomes Data of Adipose Stem Cells to Treat Multiple Sclerosis," evaluates the quality of life changes measured by the Multiple Sclerosis ...
Abstract Umbilical cord mesenchymal stem cells (MSCs) are a unique, accessible, and non-controversial source of early stem cells that can be readily manipulated. As the most common pluripotent cell, bone marrow-derived MSCs display limitations with the progress of stem cell therapy. By contrast, umbilical cord-derived cells, which have plentiful resources, are more accessible. However, several uncertain aspects, such as the effect of donor selection or culture conditions, long-term therapeutic effects, product consistency, and potential tumorigenicity, are the bottleneck in this clinical therapy. MSCs are predicted to undergo an unprecedented development in clinical treatment when a generally acknowledged criterion emerges. In the current paper, we highlight the application of umbilical cord-derived MSCs in skin therapies based on our previous studies, as well as the ...
Yu, B., Zhao, X., Yang, C., Crane, J., Xian, L., Lu, W., Wan, M. and Cao, X. (2012), Parathyroid hormone induces differentiation of mesenchymal stromal/stem cells by enhancing bone morphogenetic protein signaling. J Bone Miner Res, 27: 2001-2014. doi: 10.1002/jbmr.1663 ...
Mesenchymal stem cells that come from different cell sources can look similar but behave differently. Bone marrow-derived mesenchymal stem cells tend to be the gold standard for isolating and using mesenchymal stem cells, it is not particularly easy to access these cells from the bone marrow. Because there are other, much more easily accessible mesenchymal stem cells, such as those from the umbilical cord, it is important to establish the differences between the different types of stem cells so that each can be used when most appropriate and when most advantageous.. One important difference is how to isolate the cells and how easy it is to do so. Recent work published this year in Stem Cells and Development helped to define the best way to isolate mesenchymal stem cells from the Whartons jelly of umbilical cords. The researchers also looked ...
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Apparently, distinct from other types of stem cells, with allogeneic mesenchymal stem cells there is no danger of the childs body rejecting the stem cells given to them. This is owed to the fact that these types of cells have no antigenicity. This remarkable attribute eradicates the need to use certain drugs that suppress the immune system and can be needed with some stem cell treatments. Cord blood, or CD34+, is known to stimulate living/normal cells and tissue causing them to work at a higher level of functioning.. In Conclusion. No matter how well known or unknown, the fact is that this treatment is used worldwide. Stem Cell Treatment is being very thoroughly studied from the U.S. to China to India. It has been all the way back to 2007 or before it seems. Theres an article called Stem Cell Therapy for Autism, published 6/27/2007 in the ...
TY - JOUR. T1 - Intrathecal administration of autologous mesenchymal stem cells in multiple system atrophy. AU - Singer, Wolfgang. AU - Dietz, Allan B.. AU - Zeller, Anita D.. AU - Gehrking, Tonette L.. AU - Schmelzer, James D.. AU - Schmeichel, Ann M.. AU - Gehrking, Jade A.. AU - Suarez, Mariana D.. AU - Sletten, David M.. AU - Minota Pacheco, Karla V.. AU - Coon, Elizabeth A.. AU - Sandroni, Paola. AU - Benarroch, Eduardo E.. AU - Fealey, Robert D.. AU - Matsumoto, Joseph Y.. AU - Bower, James H.. AU - Hassan, Anhar. AU - McKeon, Andrew. AU - Windebank, Anthony J.. AU - Mandrekar, Jay N.. AU - Low, Phillip A.. PY - 2019/7/2. Y1 - 2019/7/2. N2 - OBJECTIVE: This phase I/II study sought to explore intrathecal administration of mesenchymal stem cells (MSCs) as therapeutic approach to multiple system atrophy (MSA). METHODS: Utilizing a dose-escalation design, we delivered between 10 and 200 million adipose-derived autologous MSCs intrathecally to patients with early MSA. ...
Human umbilical cord mesenchymal stem cells exhibit the potential to differentiate into neural cells, which have been confirmed in in vivo and in vitro experiments. There have been few clinical reports describing umbilical cord mesenchymal stem cells for treatment of cerebral hemorrhage.. To investigate the effects of hUC-MSC treatment for cerebral hemorrhage sequela, 20 patients with cerebral hemorrhage will be enrolled and receive 4 times of hUC-MSC transplantation.. ...
Successful bone tissue engineering strategy relies on robust cell culturing technique as well as profitable cellular microenvironment. For the current clinical treatment, the expansion of cells in vitro has been a promising alternative method for the bone tissue engineering strategy. In order to enhance the culture efficiency, bioreactor systems are widely researched for the different clinical purposes. Currently, rotating wall bioreactors, biaxial bioreactors, perfusion bioreactors and spinner flask systems should be widely used in the experiments and each of them should has its own advantages and disadvanges. In this project, I would like to achieve the enhanced efficiency of human fetal mesenchymal stem cells (hfMSCs) by the parametric studies in biaxial bioreactor systems for the bone tissue engineering. The hypothesis include: 1). the osteogenesis of hfMSCs can be improved by ...
Cell therapy is a potential therapeutic approach for neurodegenerative disorders, such as Alzheimer disease (AD). Neuronal differentiation of stem cells before transplantation is a promising procedure for cell therapy. However, the therapeutic impact and mechanisms of action of neuron-like cells differentiated from human umbilical cord mesenchymal stem cells in AD have not been determined. In this study, we used tricyclodecan-9-yl-xanthogenate (D609) to induce human mesenchymal stem cells isolated from Wharton jelly of the umbilical cord (HUMSCs) to differentiate into neuron-like cells (HUMSC-NCs), and transplanted the HUMSC-NCs into an AβPP/PS1 transgenic AD mouse model. The effects of HUMSC-NC transplantation on the cognitive function, synapsin I level, amyloid β-peptides (Aβ) deposition, and microglial function of the mice were investigated. We found that ...
Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and clinical research, including animal models and clinical trials.
The immunomodulatory properties of type 2 diabetic patients adipose-derived mesenchymal stem cells (D-ASCs) has not been extensively studied. In this study, we compared the immunomodulatory properties of D-ASCs and non-diabetic subjects mesenchymal stem cells (ND-ASCs) in co-culture with mixed leukocyte reaction (MLR). ASCs were isolated from adipose tissue samples of type 2 diabetic and non-diabetic subjects (age: 40-55). D-ASCs and ND-ASCs were co-cultured with two-way MLR. Peri pheral blood mononuclear ce lls (PBMCs) proliferation ratio, protein levels of IFN- γ and IL-10, mRNA expression of COX-2 , TNF- α , TGF- β 1 and IL-6 genes in MLR, D-ASCs and ND-ASCs co-cultures were assessed using XTT, ELISA and Real-time qRT-PCR, respectively. PBMCs proliferation on days 2 and 4 of D-ASCs co-culture was higher than ND-ASCs co-culture of the same days ( p , 0.001). IFN- γ level decreased on day 4 compared to day 2 of ND-ASCs co-culture, but its level had not ...
Background: First-generation autologous chondrocyte implantation has limitations, and introducing new effective cell sources can improve cartilage repair. Purpose: This study was conducted to compare the clinical outcomes of patients treated with first-generation autologous chondrocyte implantation to patients treated with autologous bone marrow-derived mesenchymal stem cells (BMSCs). Study Design: Cohort study; Level of evidence, 3. Methods: Seventy-two matched (lesion site and age) patients underwent cartilage repair using chondrocytes (n = 36) or BMSCs (n = 36). Clinical outcomes were measured before operation and 3, 6, 9, 12, 18, and 24 months after operation using the International Cartilage Repair Society (ICRS) Cartilage Injury Evaluation Package, which included questions from the Short-Form Health Survey, International Knee Documentation Committee (IKDC) subjective knee evaluation form, Lysholm knee scale, and Tegner activity level scale. Results: There was ...
Discover Miltenyi Biotecs solutions for the isolation, expansion, and differentiation of mesenchymal stem cells (MSCS), including immunomodulation assays. - San Marino
Although recent studies have demonstrated the in vitro hepatic differentiation potential of mesenchymal stem cells (MSCs), the evidence supporting the in vivo engraftment of MSCs, hepatic differentiation and improvement of hepatic function is still lacking. We investigated in vivo hepatic differentiation potential and therapeutic effect of cord blood derived-MSCs (CBMSCs) transplantation in a cirrhotic rat model. CBMSCs (2x106) were infused in Wistar rats with thioacetamide-induced chronic liver injury. Biochemical markers, liver fibrosis and engraftment of CBMSCs were assessed. Infused CBMSCs were detected in the perivascular or fibrous region of the liver and did not acquire mature hepatic phenotypes. There was no difference in biochemical markers and in the area of liver fibrosis between the experimental and placebo groups. After infusion of CBMSCs in our experimental cirrhotic rat model we did not observe an improvement of liver function and liver fibrosis. Inversely, CBMSCs could have a ...
Exosomes secreted by bone marrow mesenchymal stem cells (BMSCs) promote osteosarcoma cell proliferation and migration, while the underlying mechanism remains unknown. Since the long non-coding RNA PVT1 has been reported to be upregulated in osteosarcoma cells and contributes to its growth and metastasis, we aim to investigate whether BMSC-derived exosomes promote osteosarcoma growth and metastasis via transporting PVT1 into osteosarcoma cells. The PVT1 expression in BMSC-derived exosomes was markedly higher than that in osteosarcoma cell-derived exosomes. The co-culturing of BMSC-derived exosomes and osteosarcoma cells (Saos-2, MG-63, and MNNG/HOS cell lines) significantly raised PVT1 expression of osteosarcoma cells. The direct binding between PVT1 and the oncogenic protein ERG was confirmed using RNA immunoprecipitation and RNA pull-down assays, and the transported PVT1 ...
TY - JOUR. T1 - In vitro chondrogenesis of bone marrow-derived mesenchymal stem cells in a photopolymerizing hydrogel. AU - Williams, Christopher G.. AU - Kim, Tae Kyun. AU - Taboas, Anya. AU - Malik, Athar. AU - Manson, Paul. AU - Elisseeff, Jennifer Hartt. PY - 2003/8. Y1 - 2003/8. N2 - Mesenchymal stem cells (MSCS) from skeletally mature goats were encapsulated in a photopolymerizing poly(ethylene glycol)-based hydrogel and cultured with or without transforming growth factor β1 (TGF) to study the potential for chondrogenesis in a hydrogel scaffold system amenable to minimally invasive implantation. Chondrogenic differentiation was evaluated by histological, biochemical, and RNA analyses for the expression of cartilage extracellular matrix components. The two control groups studied were MSCs cultured in monolayer and MSCs encapsulated in the hydrogel and cultured for 6 weeks in chondrogenic medium without TGF-β1 (6wk-TGF). The three ...
The immunomodulatory potential of cell therapies using adipose-derived stem cells (ASCs) and bone marrow-derived mesenchymal stem cells (BM-MSCs) has been studied in vascularized composite allotransplantation (VCA). Most cell therapy-based experimental and clinical protocols integrate some degree of recipient conditioning/induction with antibodies or other immunosuppressive agents. We investigated the susceptibility of ASCs and BM-MSCs to anti-lymphocyte serum (ALS) and tacrolimus. Rat ASCs and BM-MSCs were exposed to varying concentrations of tacrolimus and ALS in vitro. Serum from ALS-treated animals was added to cell cultures. Viability, susceptibility, and cytotoxicity parameters were evaluated. ALS inhibited ASC and BM-MSC viability and susceptibility in vitro in a dose-dependent manner. ASCs were more susceptible to both ALS and tacrolimus than BM-MSCs. Trypsinized and adherent ASCs ...
Multipotent adult progenitor cells are a recently described population of stem cells derived from the bone marrow stroma. multipotent adult progenitor cells along mesodermal lineages and exhibited the enhanced expression of alkaline phosphatase and production of calcium-containing mineral debris by multipotent adult progenitor cells, necessary precursors for osteogenesis. In combination with a demineralized bone matrix scaffold, multipotent adult progenitor cells exhibited enhanced revascularization and new bone formation in vivo in an orthotopic defect model when compared to mesenchymal stem cells on demineralized bone matrix or demineralized bone matrixConly control groups. The potent combination of angiogenic and osteogenic properties provided by multipotent adult progenitor cells appears to create a synergistic amplification of the bone healing process. Our results indicate that ...
OBJECTIVE-Mesenchymal stem cells (MSCs) have been reported to secrete various cytokines that exhibit angiogenic and neurosupportive effects. This study was conducted to investigate the effects of MSC transplantation on diabetic polyneuropathy (DPN) in rats.. RESEARCH DESIGN AND METHODS-MSCs were isolated from bone marrow of adult rats and transplanted into hind limb skeletal muscles of rats with an 8-week duration of streptozotocin (STZ)-induced diabetes or age-matched normal rats by unilateral intramuscular injection. Four weeks after transplantation, vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) productions in transplanted sites, current perception threshold, nerve conduction velocity (NCV), sciatic nerve blood flow (SNBF), capillary number-to-muscle fiber ratio in soleus muscles, and sural nerve morphometry were evaluated.. RESULTS-VEGF and bFGF mRNA expression were significantly increased in MSC-injected thigh muscles of STZ-induced diabetic rats. ...
Fahmy, S. R., A. M. Soliman, M. E. Ansary, S. A. Elhamid, and H. Mohsen, Therapeutic efficacy of human umbilical cord mesenchymal stem cellstransplantation against renal ischemia/reperfusion injury in rats, Tissue & Cell, vol. 49, issue 3, pp. 369-375, 2017 ...
Zhang, Z.-Y., Chong, M.S.K., Teoh, S.-H., Schantz, J.T., Fisk, N.M., Choolani, M.A., Chan, J. (2009). Superior osteogenic capacity for bone tissue engineering of fetal compared with perinatal and adult mesenchymal stem cells. Stem Cells 27 (1) : 126-137. [email protected] Repository. https://doi.org/10.1634/stemcells.2008- ...
Human mesenchymal stem cells (MSCs) are multipotent and exert anti-inflammatory effects, but the underlying mechanism remains to be elucidated. In the current study, we investigated the regulatory mechanism of regulatory T cell (Treg) induction through the growth factors released by human MSCs. Human naive CD4+ T cells were stimulated with anti-CD3/28 Abs and cocultured with human MSC culture supernatant for 48 h. The proliferation and cytokine production of CD4+ T cells and surface molecule expression on CD4+ T cells were evaluated. The proliferation of anti-CD3/28 Abs-stimulated CD4+ T cells was suppressed by the addition of human MSC culture supernatant; in addition, the production of IL-10 and IL-4 increased. The human MSC culture supernatant induced CD4+FOXP3+ Tregs that expressed CD25, CTLA-4, glucocorticoid-induced TNFR-related protein, insulin-like growth factor (IGF)-1R, and IGF-2R, showing ...
Preterm newborns are at high risk of developing neurodevelopmental deficits caused by neuroinflammation leading to perinatal brain injury. Human Whartons jelly mesenchymal stem cells (hWJ-MSC) derived from the umbilical cord have been suggested to reduce neuroinflammation, in part through the release of extracellular vesicle-like exosomes. Here, we studied whether exosomes derived from hWJ-MSC have anti-inflammatory effects on microglia-mediated neuroinflammation in perinatal brain injury. Using ultracentrifugation, we isolated exosomes from hWJ-MSC culture supernatants. In an in vitro model of neuroinflammation, we stimulated immortalized BV-2 microglia and primary mixed glial cells with lipopolysaccharide (LPS) in the presence or absence of exosomes. In vivo, we introduced brain damage in 3-day-old rat pups and treated them intranasally with hWJ-MSC-derived exosomes. hWJ-MSC-derived exosomes dampened the LPS-induced expression of inflammation-related genes ...
Dr. Cohens study utilizes the CTL based in the Case Western Reserve University School of Medicine NCRM, which is the only academic cleanroom facility for cellular therapy procedures in Northeast Ohio. The role of the CTL is to culture expand or "manufacture" the human MSCs for reinfusion into study subjects.. This study requires a specialized Class 10,000 cleanroom environment and uniquely qualified personnel because the study subjects marrow cells need to be grown ex vivo (outside of the body) for approximately a month to achieve an adequate number of MSCs. Once the desired dose is obtained, the cells are tested extensively to confirm they are viable and not contaminated to ensure safety, then cryopreserved. At a later date, they are transported to Mellen Center, carefully thawed, and infused intravenously. This type of cell manipulation is performed under the authority of the US Food and Drug ...
Mesenchymal stem cells (MSCs) are immunosuppressive, but we lack an understanding of how these adult stem cells are in turn affected by immune cells and the surrounding tissue environment. As MSCs have stromal functions and exhibit great plasticity, the influence of an inflamed microenvironment on their responses is important to determine. MSCs downregulate microglial inflammatory responses, and here we describe the mutual effects of coculturing mouse bone marrow MSCs with BV2 microglia in a lipopolysaccharide (LPS) inflammatory paradigm. Mouse MSCs were cultured from femoral and tibial bone marrow aspirates and characterized. MSCs were cocultured with BV2 microglia at four seeding-density ratios (1:0.2, 1:0.1, 1:0.02, and 1:0.01 (BV2/MSC)), and stimulated with 1 μg/ml LPS. In certain assays, MSCs were separated from BV2 cells with a cell-culture insert to determine the influence of soluble factors on ...
Multipotent mesenchymal stromal cells (MSCs) have potential therapeutic benefit for the treatment of neurological diseases and injury. MSCs interact with and alter brain parenchymal cells by direct cell-cell communication and/or by indirect secretion of factors and thereby promote functional recover …
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In the clinic, bone defects resulting from infections, trauma, surgical resection and genetic malformations remain a significant challenge. In the field of bone tissue engineering, three-dimensional (3D) scaffolds are promising for the treatment of bone defects. In this study, calcium sulfate hydrate (CSH)/mesoporous bioactive glass (MBG) scaffolds were successfully fabricated using a 3D printing technique, which had a regular and uniform square macroporous structure, high porosity and excellent apatite mineralization ability. Human bone marrow-derived mesenchymal stem cells (hBMSCs) were cultured on scaffolds to evaluate hBMSC attachment, proliferation and osteogenesis-related gene expression. Critical-sized rat calvarial defects were applied to investigate the effect of CSH/MBG scaffolds on bone regeneration in vivo. The in vitro results showed that CSH/MBG scaffolds stimulated the adhesion, proliferation, alkaline phosphatase (ALP) activity and ...
TY - JOUR. T1 - Interleukin-10 delivery via mesenchymal stem cells. T2 - A novel gene therapy approach to prevent lung ischemia-reperfusion injury. AU - Manning, Eddie. AU - Pham, Si. AU - Li, Sen. AU - Vazquez-Padron, Roberto I. AU - Mathew, James. AU - Ruiz, Phillip. AU - Salgar, Shashikumar K.. PY - 2010/6/1. Y1 - 2010/6/1. N2 - Ischemia-reperfusion (IR) injury is an important cause of primary graft failure in lung transplantation. In this study, viral interleukin-10 (vIL-10)-engineered mesenchymal stem cells (MSCs) were tested for their ability to prevent lung IR injury. Bone marrow-derived MSCs were transduced with rvIL-10-retrovirus. After 120min of warm left lung ischemia, rats received ∼15×106 vIL-10-engineered MSCs (MSC-vIL-10), empty vector-engineered MSCs (MSC-vec), or saline intravenously. Mean blood oxygenation (PaO2/FiO2 ratio, mmHg) was measured at 4hr, 24hr, 72hr, and 7 days. As early as 4hr post-IR injury with MSC-vIL-10 treatment, blood ...
Inflammatory skin disorders that cause serious deterioration of the quality of life have become one of the major public concerns. Despite their significance, there is no fundamental cure to date. Mesenchymal stem cells (MSCs) possess unique immunomodulatory properties which make them a promising tool for the treatment of various inflammatory diseases. Our recent preclinical and clinical studies have shown that MSCs can be successfully used for the treatment of atopic dermatitis (AD), one of the major inflammatory skin diseases. This observation along with similar reports from other groups revealed the efficacy and underlying mechanisms of MSCs in inflammatory dermatosis. In addition, it has been proposed that cell priming or gene transduction can be novel strategies for the development of next-generation high-efficacy MSCs for treating inflammatory skin diseases. We discuss here existing evidence that demonstrates the regulatory properties of MSCs on immune responses under ...
TY - JOUR. T1 - Overexpression of human arginine decarboxylase rescues human mesenchymal stem cells against H2O2 toxicity through cell survival protein activation. AU - Seo, Su Kyoung. AU - Yang, Wonsuk. AU - Park, Yu Mi. AU - Lee, Won Taek. AU - Park, Kyung Ah. AU - Lee, Jong Eun. PY - 2013/3/1. Y1 - 2013/3/1. N2 - In this study, we explored the potentiality of human arginine decarboxylase (ADC) to enhance the survival of mesenchymal stem cells (MSCs) against unfavorable milieu of host tissues as the low survival of MSCs is the issue in cell transplantation therapy. To address this, human MSCs overexpressing human ADC were treated with H2O2 and the resultant intracellular events were examined. First, we examined whether human ADC is overexpressed in human MSCs. Then, we investigated cell survival or death related events. We found that the overexpression of human ADC ...
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TY - JOUR. T1 - Cross-linked iron oxide nanoparticles for therapeutic engineering and in vivo monitoring of mesenchymal stem cells in cerebral ischemia model. AU - Park, Ji Won. AU - Ku, Sook Hee. AU - Moon, Hyung Ho. AU - Lee, Minhyung. AU - Choi, Donghoon. AU - Yang, Jaemoon. AU - Huh, Yong Min. AU - Jeong, Ji Hoon. AU - Park, Tae Gwan. AU - Mok, Hyejung. AU - Kim, Sun Hwa. PY - 2014/1/1. Y1 - 2014/1/1. N2 - Poly(ethylene glycol)-coated cross-linked iron oxide nanoparticles (PCIONs) are developed for therapeutic engineering of mesenchymal stem cells (MSCs) and their monitoring via magnetic resonance (MR) imaging at a time. PCIONs successfully combine with plasmid DNA (pDNA) via ionic interaction. Accordingly, PCION/pDNA complexes mediate superior translocations of vascular endothelial growth factor (VEGF) pDNA into intracellular regions of MSCs under external magnetic field, which significantly elevate production of VEGF from MSCs. Genetically engineered ...
Objective: The hepatic integration of human adipose tissue derived mesenchymal stem cells (hAT-MSCs) in vivo with or without prior differentiation to hepatocyte-like cells in vitro was investigated.. Methods and results: Cells, isolated either from peritoneal or subcutaneous adipose tissue, expressed mesenchymal stem cell surface markers and featured multiple lineage differentiation. Under conditions favouring hepatocyte differentiation, hAT-MSCs gained hepatocytic functions in vitro including urea formation, glycogen synthesis, cytochrome P450 enzyme activity, and expression of hepatocyte-specific transcripts of carbamoylphosphate synthetase, albumin and cytochrome P450 type 3A4 (CYP3A4). Transgenic expression of green fluorescent protein emerged upon hepatocyte differentiation when driven by the hepatocyte-specific promoter of the cytosolic phosphoenolpyruvate carboxykinase gene but was constitutive from ...
TY - JOUR. T1 - Transplantation of Cryopreserved Human Bone Marrow-derived Multipotent Adult Progenitor Cells for Neonatal Hypoxic-Ischemic Injury. T2 - Targeting the Hippocampus. AU - Yasuhara, Takao. AU - Matsukawa, Noriyuki. AU - Yu, Guolong. AU - Xu, Lin. AU - Mays, Robert W.. AU - Kovach, Jim. AU - Deans, Robert. AU - Hess, David C. AU - Carroll, James Edwin. AU - Borlongan, Cesar V.. PY - 2006/1/1. Y1 - 2006/1/1. N2 - There is currently no treatment for neonatal hypoxic-ischemic (HI) injury. Although limited clinical trials of stem cell therapy have been initiated in a number of neurological disorders, the preclinical evidence of a cell-based therapy for neonatal HI injury remains in its infancy. Stem cell therapy, via stimulation of endogenous stem cells or transplantation of exogenous stem cells, has targeted neurogenic sites, such ...
TY - JOUR. T1 - Cell-cell contact with proinflammatory macrophages enhances the immunotherapeutic effect of mesenchymal stem cells in two abortion models. AU - Li, Yanhong. AU - Zhang, Di. AU - Xu, Ling. AU - Dong, Lin. AU - Zheng, Ji. AU - Lin, Yikong. AU - Huang, Jiefang. AU - Zhang, Yanyun. AU - Tao, Yu. AU - Zang, Xingxing. AU - Li, Dajin. AU - Du, Meirong. PY - 2019/12/1. Y1 - 2019/12/1. N2 - Mesenchymal stem cells (MSCs), which are pluripotent cells with immunomodulatory properties, have been considered good candidates for the therapy of several immune disorders, such as inflammatory bowel diseases, concanavalin A-induced liver injury, and graft-versus-host disease. The embryo is a natural allograft to the maternal immune system. A successful pregnancy depends on the timely extinction of the inflammatory response induced by embryo implantation, followed by the switch to a tolerant immune ...
Purpose : Mesenchymal stem cell (MSC)-based therapy is under investigation as a treatment strategy to promote tissue repair and to modulate the immune system in immune mediated disorders. MSCs have been isolated from many tissues most commonly from the bone marrow or fat. Given the cumbersome procedures for harvesting bone marrow (BM), and to a lesser extent fat, there is a need to look for other tissues for MSC isolation. The International Society for Cellular Therapy (ISCT) has established three criteria for characterizing human MSCs: adherence to plastic, differentiation potential, and the presence or absence of certain cell surface markers. We evaluated whether or not corneal limbal stromal cells (CLSC) met ISCT criteria by comparing them to BM-MSCs. Methods : BM-MSCs were isolated from healthy human bone marrow donors. CLSC were ...
Here, we aimed to investigate osteogenic differentiation of human adipose-derived stem cells (hASCs) in three-dimensional (3D) bioprinted tissue constructs in vitro and in vivo. A 3D Bio-plotter dispensing system was used for building 3D constructs. Cell viability was determined using live/dead cell staining. After 7 and 14 days of culture, real-time quantitative polymerase chain reaction (PCR) was performed to analyze the expression of osteogenesis-related genes (RUNX2, OSX, and OCN). Western blotting for RUNX2 and immunofluorescent staining for OCN and RUNX2 were also performed. At 8 weeks after surgery, osteoids secreted by osteogenically differentiated cells were assessed by hematoxylin-eosin (H&E) staining, Masson trichrome staining, and OCN immunohistochemical staining. Results from live/dead cell staining showed that most of the cells remained alive, with a cell ...
TY - JOUR. T1 - Bone marrow-derived stem cells and hepatocarcinogenesis in hepatitis B virus transgenic mice. AU - Barone, Michele. AU - Scavo, Maria Principia. AU - Maiorano, Eugenio. AU - Di Leo, Alfredo. AU - Francavilla, Antonio. PY - 2014/3. Y1 - 2014/3. N2 - Background: Several studies have demonstrated that cancer can develop with the contribution of bone marrow-derived cancer stem cells. We evaluated the possible involvement of bone marrow-derived stem cells in hepatocarcinogenesis in a hepatitis B virus (HBV) transgenic mouse model. Methods: Bone marrow cells from wild type male mice were transplanted into sublethally irradiated, female, HBV transgenic mice with hepatocarcinoma nodules. Four months later, liver tissue was examined to localize neoplastic nodules/foci and characterize cells by evaluating the Y-chromosome and the hepatocyte lineage marker hepatocyte nuclear factor-1 (HNF1), as well as ...
In this thesis, changes in Youngs modulus of mesenchymal stem cells (MSCs) were investigated during their osteogenic differentiation on bioactive peptide nanofibers that bear triple glutamic acid sequence (EEE), a non-collagenous protein sequence of some extracellular matrix (ECM) proteins (e.g. bone sialoprotein) found in bone tissue. MSCs formed spherical cell aggregates on the osteoinductive peptide nanofibers, here also called osteospheroids, of which their cells made intensive cell-cell contacts and showed osteoblast-like cell morphology. Mechanical characterization of the osteospheroids on the peptide nanofiber hydrogel was performed using atomic force microscope (AFM) where AFM probes modified with a thin film coating of octa uorocyclobutane (C4F8) were used to measure force maps of the cells at days 3, 7 and 14 of osteogenic differentiation. Hertz ...
Purpose: To compare the cellular uptake efficiency and cytotoxicity of aminosilane (SiO2-NH2)-coated superparamagnetic iron oxide ([email protected]) nanoparticles with three other types of SPIO nanoparticles coated with SiO2 ([email protected]), dextran ([email protected]), or bare SPIO in mammalian cell lines. Materials and methods: Four types of monodispersed SPIO nanoparticles with a SPIO core size of 7 nm and an overall size in a range of 7-15 nm were synthesized. The mammalian cell lines of MCF-7, MDA-MB-231, HT-29, RAW264.7, L929, HepG2, PC-3, U-87 MG, and mouse mesenchymal stem cells (MSCs) were incubated with four types of SPIO nanoparticles for 24 hours in the serum-free culture medium Dulbeccos modified Eagles medium (DMEM) with 4.5 μg/mL iron concentration. The cellular uptake efficiencies of SPIO nanoparticles were compared by Prussian blue staining and intracellular iron quantification. In vitro magnetic ...
The purpose of this study was to develop a fibrin gel system capable of serving as a three dimensional scaffold for the chondrogenesis of rabbit bone marrow mesenchymal stem cells (BM-MSCs) and to examine the effect of two fibrinolytic inhibitors, aprotinin and aminohexanoic acid, on this system. Rabbit BM-MSCs were obtained from the tibias and femurs of New Zealand white rabbits. After chondrogenic potential of BM-MSCs was verified by pellet culture, 2 x 106 cells were pelleted and suspended in fibrinogen (80mg/ml) and then mixed with equal parts of thrombin (5 IU/ml). The specimen were then divided into four groups: aprotinin control (with aprotinin); aprotinin + transforming growth factor (TGF-beta) (with aprotinin and TGF-beta 1); amino control (with aminohexanoic acid); and amino+TGF-beta (with aminohexanoic acid and TGF- beta1). Each of these groups was further divided into three groups depending on the concentration of the inhibitor. Both of the aprotinin groups ...
Aim. This project studies the regeneration of epidermal and dermal components with the use human Whartons Jelly Mesenchymal Cells (WJ-MSCs) on a scaffold of Freeze Dried Human Amniotic Membrane (FD-HAM) and Cryopreserved Human Amniotic Membrane (CP-HAM) in porcine excisional wound model. Methods. Fresh AM was harvested from seronegative term pregnant mothers undergoing elective Caesarean section deliveries under sterile conditions. The membrane was de-cellularised and either cryopreserved (CP-HAM) or freeze dried (FD-HAM) for storage. Commercially available WJ-MSCs were seeded on the different forms of the decellularised membrane. Six, 5×5 cm cutaneous wounds using a dermatome at 0.8mm setting, 2cm apart will be created on the dorsum of 20-25kg Great White pigs on either side of the spine under general anaesthesia. The treatments (Wound control, WJ-MSCs alone, FD-HAM, CP-HAM, FD-HAM + WJ-MSCs, CP-HAM+ WJ-MSCs) were allocated by Latin squares to randomise the ...
TY - JOUR. T1 - Development of bioactive peptide amphiphiles for therapeutic cell delivery. AU - Webber, Matthew J.. AU - Tongers, Jörn. AU - Renault, Marie Ange. AU - Roncalli, Jerome G.. AU - Losordo, Douglas W.. AU - Stupp, Samuel I.. PY - 2010/1/1. Y1 - 2010/1/1. N2 - There is great clinical interest in cell-based therapies for ischemic tissue repair in cardiovascular disease. However, the regenerative potential of these therapies is limited due to poor cell viability and minimal retention following application. We report here the development of bioactive peptide amphiphile nanofibers displaying the fibronectin-derived RGDS cell adhesion epitope as a scaffold for therapeutic delivery of bone marrow derived stem and progenitor cells. When grown on flat substrates, a binary peptide amphiphile system consisting of 10 wt.% RGDS-containing molecules and 90 wt.% negatively charged diluent ...
Objectives: Human bone marrow stromal cells (hBMSCs) are adherent fibroblast-like cells found in the bone marrow. They are a heterogeneous population of cells that includes a subset of osteoprogenitors. BMSCs have been widely used for tissue engineering, especially for bone regeneration. However, for clinical application currently, large quantities of hBMSCs are usually required for transplantation which is typically produced by serial passages of the cells ex vivo. We examined the effects of in vitro expansion on hBMSCs proliferation, multidifferentiation, and gene expression profiles. Methods: hBMSCs were harvested from surgical waste bone specimens from 3 healthy adults with IRB approval. The hBMSCs were cultured in α-MEM with 10% FBS and 1% penicillin-streptomycin. hBMSCs were trypsinized and passaged when they reached 70-80% confluence. Cells from early passage (p2 or 3) were compared with late passage ...
Although transplanting mesenchymal stem cells can improve cardiac function and contribute to endothelial recovery in a damaged artery, mesenchymal stem cells may induce neointimal hyperplasia by directly or indirectly acting ...
One of the conventional approaches in tissue engineering is the use of scaffolds in combination with cells to obtain mechanically stable tissue constructs in vitro prior to implantation. Additive manufacturing by fused deposition modeling is a widely used technique to produce porous scaffolds with defined pore network, geometry, and therewith defined mechanical properties. Bone marrow derived mesenchymal stromal cells (MSCs) are promising candidates for tissue engineering based cell therapies due to their multipotent character. One of the hurdles to overcome when combining additive manufactured scaffolds with MSCs is the resulting heterogeneous cell distribution and limited cell proliferation capacity. In this study, we show that the use of a biaxial rotating bioreactor, after static culture of human fetal MSCs (hfMSCs) seeded on synthetic polymeric ...
Focal adhesions (FAs) undergo maturation culminating in size and composition changes that modulate adhesion, cytoskeleton remodeling and differentiation. While it is well-recognized that stimuli for osteogenesis of mesenchymal stem cells (MSCs) drive FA maturation, actin organization, and stress-fiber polarization, the extent to which FA-mediated signals regulated by the FA protein composition specifies MSC commitment remains largely unknown. Here we demonstrate that, upon dexamethasone (osteogenic induction) treatment, guanine nucleotide exchange factor-H1 (GEF-H1) is significantly enriched in FAs. Perturbation of GEF-H1 inhibits FA formation, anisotropic stress-fiber orientation and MSC osteogenesis in an actomyosin contractility-independent manner. To determine the role of GEF-H1 in MSC osteogenesis, we explore the GEF-H1-modulated FA proteome that reveals non-muscle myosin-II heavy chain-B (NMIIB) as a target of GEF-H1 in FAs. Inhibition of targeting NMIIB into FAs suppresses FA ...
Umbilical cord blood is taken from a new-borns umbilical cord and placenta immediately following birth - a painless, risk-free procedure. Cord blood contains a high concentration of stem cells, and recent studies have shown it provides unique advantages over traditional bone-marrow transplantation. The most significant advantage is that the donor cord blood stem cells do not have to match 100% to the recipients tissue, making finding a suitable match easier.. Cord blood can be used for the baby, the brothers and sisters and the mom and dad with a suitably matching tissue type, and in these instances cord blood stem cell transplants can in fact prove life-saving.. Much like cord blood, umbilical cord tissue can also be collected and stored in a stem cell bank. Cord tissue Mesenchymal stem cells (MSCs) are younger and considered a superior resource when ...
The therapeutic strategies for neurodegenerative diseases still represent a vast research field because of the lack of targeted, effective and resolutive treatment for neurodegenerative diseases. The use of stem cell-based therapy is an alternative approach that could lead to the replacement of damaged neuronal tissue. For this purpose, adult mesenchymal stem cells (MSC), including periodontal ligament stem cells (PDLSCs), could be very useful for their differentiation capacity, easy isolation and the ability to perform an autologous implant. The aim of this work was to test whether the Moringin [4-(α-L-rhamnosyloxy) benzyl isothiocyanate; GMG-ITC], an isothiocyanate extracted from Moringa oleifera seeds, was able to induce PDLSCs toward neural progenitor differentiation. Next-generation transcriptomics sequencing showed that moringin treatment increased the expression of genes involved in neuron cortical ...
Magnussen RA, Granan LP, Dunn WR, Amendola A, Andrish JT, Brophy R, Carey JL, Flanigan D, Huston LJ, Jones M, Kaeding CC, McCarty EC, Marx RG, Matava MJ, Parker RD, Vidal A, Wolcott M, Wolf BR, Wright RW, Spindler KP, Engebretsen L. Cross-cultural comparison of patients undergoing ACL reconstruction in the United States and Norway. Knee Surg Sports Traumatol Arthrosc. 2010 Jan;18(1):98-105.. Murray SJ, Santangelo KS, Bertone AL. Evaluation of early cellular influences of bone morphogenetic proteins 12 and 2 on equine superficial digital flexor tenocytes and bone marrow-derived mesenchymal stem cells in vitro. Am J Vet Res. 2010 Jan;71(1):103-14.. Brophy RH, Zeltser D, Wright RW, Flanigan DC. Anterior cruciate ligament reconstruction and concomitant articular cartilage injury: incidence and treatment. Arthroscopy 2010 Jan;26(1):112-20.. Marras WS, Lavender SA, Ferguson SA, Splittstoesser RE, Yang G, Schabo P. Instrumentation for measuring dynamic spinal load moment exposures ...
The human body develops from a single cell, the zygote, the product of the maternal oocyte and the paternal spermatozoon. That 1-cell zygote embryo will divide and eventually grow into an adult human which is comprised of ~3.7x1013 cells. The tens of trillions of cells in the adult human can be classified into approximately 200 different highly specialized cell types that make up all of the different tissues and organs of the human body. Regenerative medicine aims to replace or restore dysfunctional cells, tissues and organs with fully functional ones. One area receiving attention is regeneration of the intervertebral discs (IVDs), which are located between the vertebrae and function to give flexibility and support load to the spine. Degenerated discs are a major cause of lower back pain. Different stem cell based regenerative medicine ...
U.S., Jan. 13 -- ClinicalTrials.gov registry received information related to the study (NCT03014037) titled Comparing Mesenchymal Stem Cell Counts in Unilateral vs. Bilateral Posterior Superior Iliac Spine Bone Marrow Aspiration on Jan. 5. Brief Summary: Musculoskeletal conditions are common sources of pain. Specifically, osteoarthritis affects nearly 27 million people in the United States. This disease leads to the breakdown of the cartilage surfaces of the joints, which serve as the connection points between the bones of the body, causing a large amount of pain and disability in patients. Increasing the number of available treatment options for osteoarthritis is becoming increasingly more important as Americans continue to age. Using stem cells taken from bone marrow is one of the non-operative treatments that has been gaining popularity in the recent past. This study aims to clarify how this procedure should best be done by comparing stem cell ...
Here, we demonstrate that bone marrow-derived stem cells engraft the murine endometrium. Both stromal and epithelial cells were derived from bone marrow origin. These data show the potential for stem cells to have a role in the regeneration or repair of this tissue after injury. However, the small number of engrafted cells limits their potential to significantly contribute to cyclic endometrial function during each estrus cycle. In other organs, the homing and engraftment of stem cells are influenced by injury and inflammation, presumably through the generation of a signal emanating from the damaged tissue [17, [18]-19]. A more significant engraftment of endometrium by bone marrow is likely to occur after endometrial injury or inflammatory insult. Additionally, the proliferation and development of endometrium are entirely regulated by hormonal stimuli. Ovarian estrogen and progesterone drive ...
This review discusses the role of the cannabinoid system in cartilage tissue and endeavors to establish if targeting the cannabinoid system has potential in mesenchymal stem cell based tissue-engineered cartilage repair strategies. The review discusses the potential of cannabinoids to protect against the degradation of cartilage in inflamed arthritic joints and the influence of cannabinoids on the chondrocyte precursors, mesenchymal stem cells (MSCs). We provide experimental evidence to show that activation of the cannabinoid system enhances the survival, migration and chondrogenic differentiation of MSCs, which are three major tenets behind the success of a cell-based tissue-engineered cartilage repair strategy. These findings highlight the potential for cannabinoids to provide a dual function by acting as anti-inflammatory agents as well as regulators of MSC biology in ...
Epigenetic changes (i.e., chromatin modifications) occur during chondrogenesis and in osteoarthritis (OA). We investigated the effect of H3K27me3 demethylase inhibition on chondrogenesis and assessed its utility in cartilage tissue engineering and in understanding cartilage destruction in OA. We used a high-content screen to assess the effect of epigenetic modifying compounds on collagen output during chondrogenesis of monolayer human mesenchymal stem cells (MSCs). The impact of GSK-J4 on gene expression, glycosaminoglycan output and collagen formation during differentiation of MSCs into cartilage discs was investigated. Expression of lysine (K)-specific demethylase 6A (UTX) and Jumonji domain-containing 3 (JMJD3), the HEK27Me3 demethylases targeted by GSK-J4, was measured in damaged and undamaged cartilage from patients with OA. The impact of GSK-J4 on ex vivo cartilage destruction and expression of OA-related genes in human articular chondrocytes (HACs) was assessed. ...
BACKGROUND: Epigenetic changes (i.e., chromatin modifications) occur during chondrogenesis and in osteoarthritis (OA). We investigated the effect of H3K27me3 demethylase inhibition on chondrogenesis and assessed its utility in cartilage tissue engineering and in understanding cartilage destruction in OA. METHODS: We used a high-content screen to assess the effect of epigenetic modifying compounds on collagen output during chondrogenesis of monolayer human mesenchymal stem cells (MSCs). The impact of GSK-J4 on gene expression, glycosaminoglycan output and collagen formation during differentiation of MSCs into cartilage discs was investigated. Expression of lysine (K)-specific demethylase 6A (UTX) and Jumonji domain-containing 3 (JMJD3), the HEK27Me3 demethylases targeted by GSK-J4, was measured in damaged and undamaged cartilage from patients with OA. The impact of GSK-J4 on ex vivo cartilage destruction and expression of OA-related genes in human articular chondrocytes ...
Whartons jelly stem cells (WJSCs) are a potential source of transplantable stem cells in cartilage-regenerative strategies, due to their highly proliferative and multilineage differentiation capacity. We hypothesized that a non-direct co-culture system with human articular chondrocytes (hACs) could enhance the potential chondrogenic phenotype of hWJSCs during the expansion phase compared to those expanded in monoculture conditions. Primary hWJSCs were cultured in the bottom of a multiwell plate separated by a porous transwell membrane insert seeded with hACs. No statistically significant differences in hWJSCs duplication number were observed under either of the culture conditions during the expansion phase. hWJSCs under co-culture conditions show upregulations of collagen type I and II, COMP, TGFβ1 and aggrecan, as well as of the main cartilage transcription factor, SOX9, when compared to those cultured in the absence of chondrocytes. Chondrogenic differentiation of hWJSCs, ...
There are two basic stem cell types. The first is called unlimited stem cells (also known as embryonic stem cells). These can turn into any kind of cell, while the second type is termed limited stem cells (also known as adult stem cells).. With the unlimited type of stem cells, the cells have the potential to become any human cell type. They can be replicated outside the body and have applications for many human diseases.. Limited stem cells, though, do not have the same limitless potential and cannot be replicated outside the body. They need to be either frozen or immediately transplanted into the body.. R3 stem cell clinics do not work with embryonic stem cells, only adult stem cells that exist in two varieties:. 1) Hematopoietic Stem Cells - these exist in human bone ...
Non-healing bone fractures and periodontal bone loss constitute significant clinical problems with few approved medical options. Bone repair is enhanced by the presence of osteoblasts or osteoblastic precursor cells. Subcutaneous adipose tissue is a plentiful, accessible, and replenishable source of human stromal cells for transplantation. In Phase I of this SBIR, we tested the hypothesis that human adipose tissue-derived stromal cells are capable of osteoblast function. Substantial in vitro data indicates that these stromal cells differentiate into cells biochemically and morphologically similar to osteoblasts. The ability of these cells to form bone in vivo was examined as well. Phase II of this SBIR will extend these in vivo experiments. Specific Aim 1 examines the ability of human adipose tissue-derived stromal cells to form ectopic bone ...
TY - JOUR. T1 - Stable transduction of human pancreatic adenocarcinoma cells, rat fibroblasts, and bone marrow-derived stem cells with recombinant adeno- associated virus containing the rat preproisulin II gene. AU - Bochan, M. R.. AU - Sidner, R. A.. AU - Shah, R.. AU - Cummings, O. W.. AU - Goheen, M.. AU - Jindal, R. M.. PY - 1998/1/1. Y1 - 1998/1/1. UR - http://www.scopus.com/inward/record.url?scp=0031978015&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0031978015&partnerID=8YFLogxK. U2 - 10.1016/S0041-1345(97)01353-5. DO - 10.1016/S0041-1345(97)01353-5. M3 - Article. C2 - 9532125. AN - SCOPUS:0031978015. VL - 30. SP - 453. EP - 454. JO - Transplantation Proceedings. JF - Transplantation Proceedings. SN - 0041-1345. IS - 2. ER - ...
Bone marrow stromal cells (BMSCs) constitute a cell population routinely used as a representation of mesenchymal stem cells in vitro. They reside within the bone marrow cavity alongside hematopoietic stem cells (HSCs), which can give rise to red blood cells, immune progenitors, and osteoclasts. Thus, extractions of cell populations from the bone marrow results in a very heterogeneous mix of various cell populations, which can present challenges in experimental design and confound data interpretation. Several isolation and culture techniques have been developed in laboratories in order to obtain more or less homogeneous populations of BMSCs and HSCs invitro. Here, we present two methods for isolation of BMSCs and HSCs from mouse long bones: one method that yields a mixed population of BMSCs and HSCs and one method that attempts to separate the two cell populations ...
TY - JOUR. T1 - Comparative profiling of stemness markers expressed by human umbilical vein endothelial cells and human umbilical cord matrix-derived stem cells. AU - Hayati, Abdul Rahman. AU - Adzman-Sellymiah, AU - Kien Hui, Chua. AU - Aqilah, Raman Nur Fatin. AU - Tan, Geok Chin. AU - Fatimah, Simat Siti. AU - Lim, Yun Hsuen. AU - Syahrina, Rahim Nur. AU - Fariha, Mohd Manzor Nur. PY - 2018/12/28. Y1 - 2018/12/28. N2 - Mesenchymal stem cells population has been successfully isolated from the matrix and characterised for its stemness properties. Endothelial cells from the vein of the umbilical cord which are usually termed as HUVEC are also of interest to researchers as it can be manipulated in vitro to study endothelial cells behaviour and vascular-associated diseases. Immunophenotyping for positive and negative markers of mesenchymal stem cells was performed on HUVEC and compared to the closest stem ...
Effects of silica-gentamicin nanohybrids on osteogenic differentiation of human osteoblast-like SaOS-2 cells Wei He,1 Dina A Mosselhy,2,3 Yudong Zheng,1 Qingling Feng,4 Xiaoning Li,4 Xing Yang,4 Lina Yue,1 Simo-Pekka Hannula2 1School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, Peopleâ s Republic of China; 2Department of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, Espoo, Finland; 3Microbiological Unit, Fish Diseases Department, Animal Health Research Institute, Giza, Egypt; 4State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, Peopleâ s Republic of China Introduction: In recent years, there has been an increasing interest in silica (SiO2) nanoparticles (NPs) as drug delivery systems. This interest is mainly attributed to the ease of their surface functionalization for drug loading. In orthopedic applications, ...
Introduction: Quantitative assessment of right ventricular (RV) function by echocardiography remains a challenge due to the complex geometry of the RV. The objective of this study was to assess the utility of speckle-tracking echocardiography (STE) compared to conventional transthoracic two-dimensional (2DE) in a RV pressure-overload swine model after treatment with cardiac or mesenchymal stem cells.. Methods: Neonatal swine underwent pulmonary artery banding (PAB) to induce RV dysfunction. After banding, pigs received intramyocardial injection into the RV free wall with human c-kit+ cardiac stem cells (hCSCs) alone (n=5), human mesenchymal stem cells (hMSCs) alone (n=5), a combination of hCSCs/hMSCs (n=5) or placebo (phosphate-buffered saline, n=5). Standard 2DE was performed pre-operatively, post-banding and at 4 weeks. Offline blinded analysis using vendor-independent software was performed measuring longitudinal strain and strain rate from the apical ...
Pluripotent stem cell-derived differentiated endothelial cells offer high potential in regenerative medicine in the cardiovascular system. With the aim of translating the use of a human stem cell-derived endothelial cell product (hESC-ECP) for treatment of critical limb ischemia (CLI) in man, we report a good manufacturing practice (GMP)-compatible protocol and detailed cell tracking and efficacy data in multiple preclinical models. The clinical-grade cell line RC11 was used to generate hESC-ECP, which was identified as mostly endothelial (60% CD31+/CD144+), with the remainder of the subset expressing various pericyte/mesenchymal stem cell markers. Cell tracking using MRI, PET, and qPCR in a murine model of limb ischemia demonstrated that hESC-ECP was detectable up to day 7 following injection. Efficacy in several murine models of limb ischemia ...
Results Mesodermal markers increased in EB while undifferentiated ES markers disappeared. After 21 days of chondrogenic culture in micromass pellets, GAG analysis showed that proteoglycan production was significantly greater in chondrogenic pellets than in undifferentiated hiPSCs and EBs. Safranin-O staining demonstrated that the cells in chondrogenic pellets took on the appearance of immature chondrocytes and secreted extracellular matrix. The chondrogenic marker gene and protein expression increased after 21days of pellet culture. The chondrogenic pellets derived from hiPS cells have very low expression of hypertrophic or osteogenic markers.. Also, hiPS cells underwent good chondrogenic differentiation in PLGA scaffold or alginate gel as well. When hiPS cells in either pellet state or in alginate hydrogel were implanted in the osteochondral defects created on the patellar groove of immunosuppressed rats, the defects ...
Another advantage of human embryonic stem cells is that they can be propagated indefinitely in lab cultures and provide an unlimited source of high quality mesenchymal stem cells - the kind of stem cell needed for treatment of MS, the researchers say. This ability to reliably grow high quality mesenchymal stem cells from embryonic stem cells represents an advantage over adult bone marrow stem cells, which must be obtained from a limited supply of healthy donors and are of more variable quality ...
Abstract. Results obtained from completed and on-going clinical studies indicate huge therapeutic potential of stem cell-based therapy in the treatment of degenerative, autoimmune and genetic disorders. However, clinical application of stem cells raises numerous ethical and safety concerns.. In this review, we provide an overview of the most important ethical issues in stem cell therapy, as a contribution to the controversial debate about their clinical usage in regenerative and transplantation medicine.. We describe ethical challenges regarding human embryonic stem cell (hESC) research, emphasizing that ethical dilemma involving the destruction of a human embryo is a major factor that may have limited the development of hESC-based clinical therapies. With previous derivation of induced pluripotent stem cells (iPSCs) this problem has been overcome, however ...
Several laboratories have begun to characterize the interactions between MSCs and cells of the immune system. hMSCs have a number of surface molecules that would predict interaction with T cells. MHC I, Thy-1 (CD90), vascular cell adhesion molecule (CD106, VCAM), intercellular adhesion molecule 1 (ICAM-1) and ICAM-2, activated leukocyte cell adhesion molecule (CD166, ALCAM), lymphocyte functional antigen-3, and integrins α1 to α3, α5, α6, β1, β3, and β4 (CD49 a, b, c, e, and f) are surface molecules expressed on hMSCs that have cognate ligands on T cells.21,28,50,51 Additionally, although not expressed at appreciable levels on hMSCs, MHC II expression is upregulated by incubating the cells with interferon-γ. However, the hMSCs lack the B7 costimulatory molecules CD80 and CD86, and these are not expressed after interferon treatment.52,53. Intuitively, one might expect allogeneic MSCs ...
We are going to bring in research and an important published paper from Purdue University to help understand and confirm the notion that dextrose, especially hypertonic (extra) dextrose is a significant factor in the ability of mesenchymal stem cells from bone marrow to proliferate.(2). Simply, the researchers took stem cells and glucose and put them in an experimental situation. The experiment was to see how varying levels of glucose affected stem cell numbers (proliferation). What the experiment found was that mesenchymal stem cell consumption of glucose increased proportionally with the glucose concentration in the medium. The more glucose, the more the stem cells ate, the more the stem cells multiplied.. In summary, hypertonic dextrose in published studies helps stem cell proliferation in vitro (in cultures.) While this is important, more important is what it does in the human body. We ...
BACKGROUND. Schwann cell transplantation improves post-traumatic nerve regeneration in both peripheral nervous system (PNS) and central nervous system (CNS) but sufficient numbers of immunocompatible cells are required for clinical application. Bone marrow stromal cells (BMSCs) are a readily accessible cell source that can be expanded and differentiated to specialized cells for regenerative medicine. OBJECTIVES. We attempted to establish a protocol to induce the stable differentiation of human BMSC along a Schwann cell lineage. METHODS. Neurosphere medium was used to induce human BMSCs into neurospheres. Then, these neurospheres were induced to differentiate along the Schwann cell lineage using glia growth factors, and this was followed by co-culture with dorsal root ganglion (DRG) neurons. RESULTS. A lot of spheres of floating cells appeared after human BMSCs were cultured ...
Adult stem cells are multipotent, semi-differentiated, cells that exist in all tissues. "Adult stem cell", however, is not the preferred term used in the field to refer to them-developmental biologists will usually refer to them as "tissue resident stem cells" or as a specific cell type (often referencing the genes they express). They function to maintain tissue homeostasis by dividing to replace damaged or senescent somatic cells. The two most well-studied examples of adult stem cells are the hematopoietic stem cells, which give rise to blood cells, and the intestinal crypt stem cells, which give rise to the intestinal epithelium.[1] As multipotent cells, most adult stem cell types can generate cells of several different types. However, ...

73c) Cell and Tissue-Based Therapies for Insulin-Dependent Diabetes | AIChE73c) Cell and Tissue-Based Therapies for Insulin-Dependent Diabetes | AIChE

With non-pancreatic cells, we genetically engineer hepatic and intestinal endocrine L cells for insulin secretion. These cells ... The potential and challenges for developing clinical therapies based on these approaches will be discussed. ... The critical technologies needed for such a substitute depend strongly on the type of cells used. With cells from another ... Stem and progenitor cells constitute another promising cell source, however, their reproducible differentiation into pancreatic ...
more infohttps://www.aiche.org/conferences/aiche-annual-meeting/2015/proceeding/paper/73c-cell-and-tissue-based-therapies-insulin-dependent-diabetes

Current Treatment Limitations in Age-Related Macular Degeneration and Future Approaches Based on Cell Therapy and Tissue...Current Treatment Limitations in Age-Related Macular Degeneration and Future Approaches Based on Cell Therapy and Tissue...

Submissions should focus on focusing on new diagnostic and surgical techniques, instrument and therapy updates, as well as ... many options involving cell therapy mainly based on retinal and iris pigment epithelium cells as well as stem cells are being ... Current Treatment Limitations in Age-Related Macular Degeneration and Future Approaches Based on Cell Therapy and Tissue ... 2Tissue Engineering and Biomaterials Unit, CEIT and TECNUN (University of Navarra), Paseo de Manuel Lardizabal 15, 20018 San ...
more infohttps://www.hindawi.com/journals/joph/2014/510285/abs/

Mesenchymal Stem Cell-Based HLA-Independent Cell Therapy for Tissue Engineering of Bone and Cartilage | BenthamScienceMesenchymal Stem Cell-Based HLA-Independent Cell Therapy for Tissue Engineering of Bone and Cartilage | BenthamScience

Current Stem Cell Research & Therapy. Title: Mesenchymal Stem Cell-Based HLA-Independent Cell Therapy for Tissue Engineering of ... Mesenchymal Stem Cell-Based HLA-Independent Cell Therapy for Tissue Engineering of Bone and Cartilage. Author(s): Philipp ... Keywords: Mesenchymal Stem Cells, HLA-antigen, Tissue Engineering, bone regeneration, cartilage repair ... Keywords:Mesenchymal Stem Cells, HLA-antigen, Tissue Engineering, bone regeneration, cartilage repair ...
more infohttp://www.eurekaselect.com/56675/article

Gene expression-based enrichment of live cells from adipose tissue produces subpopulations with improved osteogenic potential |...Gene expression-based enrichment of live cells from adipose tissue produces subpopulations with improved osteogenic potential |...

... of fat tissue are a particularly attractive option for cell based therapy given their accessibility and relative abundance. ... The objective of this study was to determine whether SVF cells sorted based on expression of alkaline phosphatase liver/bone/ ... Cells positive for ALPL expression (ALPL+) represented approximately 34% of the gated population, while cells negative for ALPL ... Cells were separated into positive and negative groups using fluorescence-activated cell sorting (FACS) then differentiated ...
more infohttps://stemcellres.biomedcentral.com/articles/10.1186/scrt502/figures/5

Clinical and preclinical translation of cell-based therapies using adipose tissue-derived cells | Stem Cell Research & Therapy ...Clinical and preclinical translation of cell-based therapies using adipose tissue-derived cells | Stem Cell Research & Therapy ...

... and reliable site for the isolation of adult stem cells suitable for tissue engineering and regenerative medicine applications ... The present article reviews the case reports and phase I-III clinical evidence using autologous adipose-derived cells that have ... This body of work has provided evidence supporting clinical translational applications of adipose-derived cells in safety and ... and transplantation of freshly isolated stromal vascular fraction cells and adherent, culture-expanded, adipose-derived stromal ...
more infohttps://stemcellres.biomedcentral.com/articles/10.1186/scrt19/metrics

Clinical and preclinical translation of cell-based therapies using adipose tissue-derived cells - Seattle Stem Cell CenterClinical and preclinical translation of cell-based therapies using adipose tissue-derived cells - Seattle Stem Cell Center

Clinical and preclinical translation of cell-based therapies using adipose tissue-derived cells. ... Clinical and preclinical translation of cell-based therapies using adipose tissue-derived cells ... we are hopeful that chronic diseases amenable to SVF cell therapy or ASC therapy will also be identified. ... Adipose tissue is now recognized as an accessible, abundant, and reliable site for the isolation of. adult stem cells suitable ...
more infohttps://seattlestemcellcenter.net/orthopedics/clinical-and-preclinical-translation-of-cell-based-therapies-using-adipose-tissue-derived-cells/

Autologous mesenchymal stem cells produce concordant improvements in regional function, tissue perfusion, and fibrotic burden...Autologous mesenchymal stem cells produce concordant improvements in regional function, tissue perfusion, and fibrotic burden...

Rationale: Although accumulating data support the efficacy of intramyocardial cell-based therapy to improve left ventricular ( ... abstract = "Rationale: Although accumulating data support the efficacy of intramyocardial cell-based therapy to improve left ... N2 - Rationale: Although accumulating data support the efficacy of intramyocardial cell-based therapy to improve left ... AB - Rationale: Although accumulating data support the efficacy of intramyocardial cell-based therapy to improve left ...
more infohttps://pennstate.pure.elsevier.com/en/publications/autologous-mesenchymal-stem-cells-produce-concordant-improvements

Tissue Engineering in Osteoarthritis: Current Status and Prospect of Mesenchymal Stem Cell Therapy, BioDrugs | 10.1007/s40259...Tissue Engineering in Osteoarthritis: Current Status and Prospect of Mesenchymal Stem Cell Therapy, BioDrugs | 10.1007/s40259...

Current Status and Prospect of Mesenchymal Stem Cell Therapy, BioDrugs" on DeepDyve, the largest online rental service for ... Multilineage cells from human adipose tissue: implications for cell-based therapies. Zuk, PA; Zhu, M; Mizuno, H; Huang, J; ... Tissue Engineering in Osteoarthritis: Current Status and Prospect of Mesenchymal Stem Cell Therapy. Tissue Engineering in ... Tissue Engineering in Osteoarthritis: Current Status and Prospect of Mesenchymal Stem Cell Therapy. Im, Gun-Il ...
more infohttps://www.deepdyve.com/lp/springer_journal/tissue-engineering-in-osteoarthritis-current-status-and-prospect-of-Ze1vtsBbOQ

Cell-based therapy in the repair of osteochondral defects: A novel use for adipose tissue | ScholarBank@NUSCell-based therapy in the repair of osteochondral defects: A novel use for adipose tissue | [email protected]

Cell-based therapy in the repair of osteochondral defects: A novel use for adipose tissue. Tissue Engineering 9 (4) : 733-744. ... Cell-based therapy in the repair of osteochondral defects: A novel use for adipose tissue. ...
more infohttp://scholarbank.nus.edu.sg/handle/10635/38604

Research Keyword Faculty Listing | Yale School of MedicineResearch Keyword Faculty Listing | Yale School of Medicine

Cell- and Tissue-Based Therapy. Jiangbing Zhou, PhD Associate Professor Term. Research Interests. Biocompatible Materials; ... Biomedical Engineering; Brain Injuries; Brain Neoplasms; Cell- and Tissue-Based Therapy; Drug Delivery Systems; Genetic Therapy ...
more infohttps://medicine.yale.edu/research/listing.aspx?meshId=27141

Cell therapy for wound healing.  - PubMed - NCBICell therapy for wound healing. - PubMed - NCBI

Cell-and Tissue-based Therapy; Tissue Engineering; Wounds and Injuries. PMID:. 24616577. PMCID:. PMC3945123. DOI:. 10.3346/jkms ... Cell therapy has also been developed for chronic wound healing. By transplanting cells with an excellent wound healing capacity ... Cell therapy for wound healing.. You HJ1, Han SK1.. Author information. 1. Department of Plastic Surgery, Korea University ... The purpose of this review is to provide information on clinically available cell-based treatment options for healing of acute ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/24616577?dopt=Abstract

Effective myotube formation in human adipose tissue-derived stem cells expressing dystrophin and myosin heavy chain by cellular...Effective myotube formation in human adipose tissue-derived stem cells expressing dystrophin and myosin heavy chain by cellular...

N2 - Stem cell therapy for muscular dystrophies requires stem cells that are able to participate in the formation of new muscle ... AB - Stem cell therapy for muscular dystrophies requires stem cells that are able to participate in the formation of new muscle ... Stem cell therapy for muscular dystrophies requires stem cells that are able to participate in the formation of new muscle ... abstract = "Stem cell therapy for muscular dystrophies requires stem cells that are able to participate in the formation of new ...
more infohttps://yonsei.pure.elsevier.com/en/publications/effective-myotube-formation-in-human-adipose-tissue-derived-stem-

Hypoxia-preconditioned adipose tissue-derived mesenchymal stem cell increase the survival and gene expression of engineered...Hypoxia-preconditioned adipose tissue-derived mesenchymal stem cell increase the survival and gene expression of engineered...

... indicating that this novel strategy can be used to augment the therapeutic efficacy of combined stem cell and gene therapies ... we confirmed that HP-treated adipose tissue-derived mesenchymal stem cells (HP-AT-MSCs) increased cell survival and enhanced ... we confirmed that HP-treated adipose tissue-derived mesenchymal stem cells (HP-AT-MSCs) increased cell survival and enhanced ... we confirmed that HP-treated adipose tissue-derived mesenchymal stem cells (HP-AT-MSCs) increased cell survival and enhanced ...
more infohttps://yonsei.pure.elsevier.com/en/publications/hypoxia-preconditioned-adipose-tissue-derived-mesenchymal-stem-ce

Current methods to manufacture human platelet lysates (HPLs) for cell therapy and tissue engineering: possible trends in...Current methods to manufacture human platelet lysates (HPLs) for cell therapy and tissue engineering: possible trends in...

Chou, ML & Burnouf, TPR 2017, Current methods to manufacture human platelet lysates (HPLs) for cell therapy and tissue ... Graduate Institute of Biomedical Materials and Tissue Engineering. *International Ph.D. Program for Cell Therapy and ... Current methods to manufacture human platelet lysates (HPLs) for cell therapy and tissue engineering: possible trends in ... Current methods to manufacture human platelet lysates (HPLs) for cell therapy and tissue engineering: possible trends in ...
more infohttps://tmu.pure.elsevier.com/en/publications/current-methods-to-manufacture-human-platelet-lysates-hpls-for-ce

Blood & Tissue Center Foundation Sponsors Annual Red and White Ball: Event to Raise Funding for Stem Cell Expansion Laboratory...Blood & Tissue Center Foundation Sponsors Annual Red and White Ball: Event to Raise Funding for Stem Cell Expansion Laboratory...

Using processed tissue and cell-based therapies, GenCure works to connect health care requirements with innovative solutions ... Blood & Tissue Center Foundation Sponsors Annual Red and White Ball: Event to Raise Funding for Stem Cell Expansion Laboratory ... The GenCure laboratory is designed to widen the availability of stem cells, which are used as part of therapy for certain ... GenCure, The Foundation, QualTex Laboratories and the South Texas Blood & Tissue Center are subsidiaries of San Antonio-based ...
more infohttp://www.prweb.com/releases/2015/09/prweb12985878.htm

Cardiac stem cells with electrical stimulation improve ischaemic heart function through regulation of connective tissue growth...Cardiac stem cells with electrical stimulation improve ischaemic heart function through regulation of connective tissue growth...

It is suggested that Ctgf and miR-378 are novel therapeutic targets for stem cell-based therapy. ... It is suggested that Ctgf and miR-378 are novel therapeutic targets for stem cell-based therapy.", ... It is suggested that Ctgf and miR-378 are novel therapeutic targets for stem cell-based therapy. ... It is suggested that Ctgf and miR-378 are novel therapeutic targets for stem cell-based therapy. ...
more infohttps://augusta.pure.elsevier.com/en/publications/cardiac-stem-cells-with-electrical-stimulation-improve-ischaemic-

Using Stem Cells to Grow Artificial Tissue for Peripheral Nerve RepairUsing Stem Cells to Grow Artificial Tissue for Peripheral Nerve Repair

... and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and ... Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, ... "Multilineage cells from human adipose tissue: implications for cell-based therapies," Tissue Engineering, vol. 7, no. 2, pp. ... stem cells and tissue-based devices are regulated by the Food and Drug Administration (FDA) as Human Cells and Tissue-Based ...
more infohttps://www.hindawi.com/journals/sci/2016/7502178/

Trophic factors from adipose tissue-derived multi-lineage progenitor cells promote cytodifferentiation of periodontal ligament...Trophic factors from adipose tissue-derived multi-lineage progenitor cells promote cytodifferentiation of periodontal ligament...

Stem and progenitor cells are currently being investigated for their applicability in cell-based therapy for periodontal tissue ... "Stem and progenitor cells are currently being investigated for their applicability in cell-based therapy for periodontal tissue ... Stem and progenitor cells are currently being investigated for their applicability in cell-based therapy for periodontal tissue ... Stem and progenitor cells are currently being investigated for their applicability in cell-based therapy for periodontal tissue ...
more infohttps://pure.fujita-hu.ac.jp/en/publications/trophic-factors-from-adipose-tissue-derived-multi-lineage-progeni

Upstream Single-Use Technologies Archives - Page 4 of 8 - BioProcess InternationalBioProcess International | Page 4Upstream Single-Use Technologies Archives - Page 4 of 8 - BioProcess InternationalBioProcess International | Page 4

Standards for Ancillary Materials Used in Cell- and Tissue-Based Therapies. by Fouad Atouf, PhD, Nicole M. Provost, PhD and ... Cell- and tissue-based therapies are being used increasingly to treat many diseases for which currently no other adequate ... or injured cells, tissues, or organs. Cells or tissues might be unmanipulated, or their biological characteristics can be ... Examples of cell therapies range from traditional blood transfusions to recent approaches… ...
more infohttp://www.bioprocessintl.com/category/upstream-processing/upstream-single-use-technologies/page/4/

Three-Dimensional Human Cardiac Tissue Engineered by Centrifugation of Stacked Cell Sheets and Cross-Sectional Observation of...Three-Dimensional Human Cardiac Tissue Engineered by Centrifugation of Stacked Cell Sheets and Cross-Sectional Observation of...

Biotechnology industry High technology industry Cardiology Optical tomography Methods Tissue engineering Technology application ... Three-Dimensional Human Cardiac Tissue Engineered by Centrifugation of Stacked Cell Sheets and Cross-Sectional Observation of ... Recently, tissue engineering methodology and cell-based regenerative therapy have been progressing rapidly and are attracting ... Human ES cells [36] and human iPS cells [16,37] are the focus of much new research in the fields of cell biology, tissue ...
more infohttps://www.thefreelibrary.com/Three-Dimensional+Human+Cardiac+Tissue+Engineered+by+Centrifugation+of...-a0557302217

Abstract 17004: Intramyocardial Injection of Gelatinized Capillary-Alginate Hydrogel Enhances Recovery of LV Function Post-MI |...Abstract 17004: Intramyocardial Injection of Gelatinized Capillary-Alginate Hydrogel Enhances Recovery of LV Function Post-MI |...

Core 5. Myocardium: Function and FailureSession Title: Cell, Tissue Engineering and Gene-Based Therapies V ... The diameter and density of the channels can be adjusted to facilitate cell, tissue and blood vessel ingrowth (Fig. 1A). ...
more infohttp://circ.ahajournals.org/content/126/Suppl_21/A17004

Abstract 19201: Unseeded Engineered Valve Leaflets Retain Function and Remodel After Implant in Sheep Right Pulmonary Outflow...Abstract 19201: Unseeded Engineered Valve Leaflets Retain Function and Remodel After Implant in Sheep Right Pulmonary Outflow...

Core 5. Myocardium: Function and FailureSession Title: Cell, Tissue Engineering and Gene-Based Therapies V ... cell pre-seeding may not be required for tissue formation or remodeling for a functional engineered valve; 3) host cells seem ... The majority of cells expressed α-SMA and MMP2. CD45 positive cells, suggesting hematogenous origin, were found throughout the ... Tissue engineered valves offer the potential to overcome these disadvantages by creating living structures, but is limited by ...
more infohttp://circ.ahajournals.org/content/126/Suppl_21/A19201

Health information and publications in Africa African Index Medicus DatabaseHealth information and publications in Africa African Index Medicus Database

Stem Cells Cell- and Tissue-Based Therapy Nervous System Diseases South Africa - Pretoria. ... Stem Cells Cell- and Tissue-Based Therapy Nervous System Diseases South Africa - Pretoria. ... and Tissue-Based Therapy Add the result to your basket Refine your search Generate the RSS feed of the search. Stem cell ... Many forms of stem cell therapy exist, including the use of neural, haematopoietic and mesenchymal stem cells. Cell therapies ...
more infohttp://indexmedicus.afro.who.int/aim/opac_css/index.php?lvl=more_results&mode=keyword&user_query=Cell-+and+Tissue-Based+Therapy&tags=ok

Characterization of mesenchymal stem cells derived from equine adipose tissueCharacterization of mesenchymal stem cells derived from equine adipose tissue

ZUK, P.A.; ZHU, M.; MIZUNO, H. Multi-lineage cells from human adipose tissue: implication for cell-based therapies. Tissue ... Adipose Tissue-Derived Stromal Cells-Basic and Clinical Implications for Novel Cell-Based Therapies. Stem Cells, v.25, p.818- ... Use of adipose tissue-derived mesenchymal stem cells for experimental tendinitis therapy in equines. J. Equine Vet. Sci., v.31 ... Traditionally, the process of isolating the nucleated cell fraction from the adipose tissue is based on collagenase digestion, ...
more infohttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000400001&lng=en&tlng=en

Technologies, Methods, and Products of Small Molecule Directed Tissue and Organ Regeneration from Human Pluripotent Stem Cells ...Technologies, Methods, and Products of Small Molecule Directed Tissue and Organ Regeneration from Human Pluripotent Stem Cells ...

... hold great potential for restoring tissue and organ function, which has been hindered by inefficiency and instability of ... tissue and organ engineering, tissue and organ regeneration, scale-up production, cell-based therapy, and other purposes. ... tissue and organ engineering, tissue and organ regeneration, cell-based therapy, and clinics. ... bone marrow cells, umbilical cord stem cells, cord blood cells, patients heart tissue, placenta, or fat tissue are able to ...
more infohttp://www.freepatentsonline.com/y2014/0193380.html
  • Non-pancreatic cells from the same patient (autologous), targeted by gene transfer vectors or retrieved surgically and genetically engineered ex vivo before being returned to the patient, may relax the immune acceptance problems but pose challenges regarding the amount and kinetics of insulin secretion in response to physiologic stimuli. (aiche.org)
  • These cells are potentially autologous, however, they pose challenges regarding the amount and kinetics of secreted insulin. (aiche.org)
  • The present article reviews the case reports and phase I-III clinical evidence using autologous adipose- derived cells that have been published, to date, in the fields of gastroenterology, neurology, orthopedics, reconstructive surgery, and related clinical disciplines. (seattlestemcellcenter.net)
  • Autologous and allogeneic mesenchymal stem cells (MSCs) have rapidly emerged as investigational products for cartilage regeneration. (deepdyve.com)
  • Cheek defect created by removal of a basal cell carcinoma was reconstructed by autologous fibroblasts seeded on a hyaluronic acid sheet. (nih.gov)
  • This offers a Schwann cell-rich autologous material to bridge the interstump gap and serves to guide regenerating axons. (hindawi.com)
  • In comments to the Australian Therapeutic Goods Administration (TGA), the ISSCR supported regulation of autologous products that involve more than minimal manipulation of a patient's cells and place strict limitations on the direct advertisement of cell-based therapies. (isscr.org)
  • The comments were in response to the TGA's call for input from interested parties about the regulation of autologous cell and tissue products. (isscr.org)
  • Therapeutic Vaccination With Dendritic Cells Loaded With Autologous HIV Type 1-Infected Apoptotic Cells. (nextbio.com)
  • Stem cells that can differentiate along a neural lineage represent an essential resource and starting material for this process. (hindawi.com)
  • and (iv) maintains the potential to differentiate to derivatives of endoderm, mesoderm, and ectoderm tissues throughout the culture. (freepatentsonline.com)
  • Examining the ability of these cells to proliferate and differentiate in a whole kidney extracellular matrix scaffold would serve as a valuable baseline for future studies in producing a functioning organ. (ufl.edu)
  • The ISSCR Board writes a letter to the stem cell community regarding travel to the ISSCR 2017 meeting, 14-17 June. (isscr.org)
  • Mesenchymal stem cells (MSCs) improve LV structure and function through several effects including reducing fibrosis, neoangiogenesis, and neomyogenesis. (elsevier.com)
  • This review outlines points to consider in MSC-based approaches for OA treatment, including allogeneic MSCs, sources of MSCs, dosages, feasibility of multiple injections, indication according to severity of OA lesion and patient age, and issues regarding implantation versus injection. (deepdyve.com)
  • We introduce possible mechanisms of action of implanted or injected MSCs as well as the immunological aspects of MSC therapy and provide a summary of clinical trials of MSCs in the treatment of OA. (deepdyve.com)
  • Using both in vitro and in vivo injury models, we confirmed that HP-treated adipose tissue-derived mesenchymal stem cells (HP-AT-MSCs) increased cell survival and enhanced the expression of marker genes in DsRed-engineered neural stem cells (NSCs-DsRed). (elsevier.com)
  • Similar to untreated AT-MSCs, HP-AT-MSCs had normal morphology and were positive for the cell surface markers CD90, CD105, and CD29, but not CD31. (elsevier.com)
  • The relative abundance and easy access to adipose tissue has raised great interest among researchers using these cells as a source of mesenchymal stem cells (MSCs) in equine medicine. (scielo.br)
  • 2001). The amount of MSCs within the SVF seems to be directly related to the amount of adipose tissue collected. (scielo.br)
  • Scientists are interested in the question of whether MSCs can transfer their mitochondria to somatic cells. (springer.com)
  • Cytokeratin is an epithelial cell marker and Pax-2 is necessary for kidney development. (ufl.edu)
  • Moreover, tissue engineering strategies to design and manufacture scaffolds to mimic Bruch's membrane are very diverse and under investigation. (hindawi.com)
  • Cultured on three-dimensional scaffolds according to the tissue engineering concept, they have already been successfully employed for reconstruction of mesenchymal tissues in numerous studies involving both small and large animal models. (eurekaselect.com)
  • Advances in nervous system tissue engineering technology have led to efforts to build Schwann cell scaffolds to overcome this and enhance the regenerative capacity of neurons following injury. (hindawi.com)
  • One approach to alleviate this demand is to reconstruct kidneys using tissue engineering scaffolds. (ufl.edu)
  • Based on first qualitative evidence that magnetic resonance might regenerate cartilage tissue, a number of further studies were conducted. (wikipedia.org)
  • The potential and challenges for developing clinical therapies based on these approaches will be discussed. (aiche.org)
  • Over 200 clinical studies applying various stem cell approaches to treat neurological disease have been registered to date (Clinicaltrials.gov), the majority of which are for multiple sclerosis, stroke and spinal cord injuries. (who.int)
  • Ideally, the resulting extracellular matrices would retain the organ-specific architecture and chemical composition to guide implanted cells into functional structures and eventually working organs. (ufl.edu)
  • This body of work has provided evidence supporting clinical translational applications of adipose-derived cells in safety and efficacy trials. (seattlestemcellcenter.net)
  • The next steps will include documenting the reproducibility of the current preclinical and clinical findings, and controlled testing of the safety and efficacy of ASCs and SVF cells in a range of human conditions. (seattlestemcellcenter.net)
  • Rationale: Although accumulating data support the efficacy of intramyocardial cell-based therapy to improve left ventricular (LV) function in patients with chronic ischemic cardiomyopathy undergoing CABG, the underlying mechanism and impact of cell injection site remain controversial. (elsevier.com)
  • The ISSCR supports the increase in biomedical research funding in the 21st Century Cures Act in the U.S. It also underscores concern that the bill's streamlined process for FDA review and approval of regenerative therapies include a rigorous review of scientific and clinical evidence to ensure safety and efficacy. (isscr.org)
  • With cells from another individual (allogeneic) or another species (xenogeneic), encapsulation in semipermeable barriers improves immune acceptance, as it inhibits passage of antibodies and excludes cytotoxic cells of the host. (aiche.org)
  • Since the use of allogeneic Schwann cells requires a source of nerve tissue, it is affected by the same factors that limit the autograft. (hindawi.com)
  • As discussed above, there is no shortage of diseases - both acute and chronic - where adipose-derived cell therapies could have a potential clinical impact. (seattlestemcellcenter.net)
  • The purpose of this review is to provide information on clinically available cell-based treatment options for healing of acute and chronic wounds. (nih.gov)
  • Compared with a variety of conventional methods, such as skin grafts and local flaps, the cell therapy technique is simple, less time-consuming, and reduces the surgical burden for patients in the repair of acute wounds. (nih.gov)
  • The present paper reviews the limitations of the current therapies as well as the socioeconomic impact of the AMD. (hindawi.com)
  • In our laboratory, we focus on encapsulated allo- and xenogeneic pancreatic cells and on non-pancreatic cells genetically engineered to secrete insulin in response to physiologic stimuli. (aiche.org)
  • The method according to claim 1, wherein at least 70% of the hES cells from the culture of step (i) are positive for at least 4 markers selected from the group consisting of alkaline phosphatase, Oct-4, SSEA-4, Tra-1-60, Tra-1-81, acetylated histones, Brg-1, hSNF2H, and microRNA hsa-miR-302. (freepatentsonline.com)
  • The possible mechanisms of mitochondria released from stem cells, the pathways of mitochondria transfer between the donor stem cells and recipient cells, and the internalization of mitochondria into recipient cells are discussed. (springer.com)
  • this strategy improves survival of both the transplanted cells and the host cells at the injury site. (elsevier.com)
  • Over time, histological analysis revealed distinct cell morphologies, patterns of cell division and apoptosis, and cell arrangements with Pax-2 and Cytokeratin positivity. (ufl.edu)
  • The ISSCR is saddened by the passing of Hiromitsu "Hiro" Ogawa, a generous and thoughtful man who was a great supporter of stem cell science and the ISSCR. (isscr.org)
  • The ISSCR commends the U.S. Food and Drug Administration (FDA) for reaffirming its regulatory authority over stem cell biologics and clarifying the criteria that determine which cell-based products fall under its oversight. (isscr.org)
  • The ISSCR commends the U.S. Food and Drug Administration (FDA) for its policy direction and enforcement efforts, to provide "bright lines and appropriate oversight" for regulating stem cell treatments and regenerative medicine. (isscr.org)
  • The ISSCR awards recognize outstanding scientists in the field of stem cell research and regenerative medicine. (isscr.org)
  • In an opinion editorial published today, the president of the ISSCR, Hans Clevers, underscores the importance of fetal tissue research. (isscr.org)
  • We also discuss some of the biological, practical, ethical, and commercial considerations in using these different stem cells for future clinical application. (hindawi.com)
  • However, mitochondrial transfer between mammalian cells has been discovered that challenges the current inheritance of mitochondria and mitochondrial DNA (mtDNA), providing novel thinking for some diseases with mitochondrial damage or defect. (springer.com)
  • BACKGROUND: Following the loss of hair cells from the mammalian cochlea, the sensory epithelium repairs to close the lesions but no new hair cells arise and hearing impairment ensues. (biomedsearch.com)
  • Decellularization of whole rat kidneys was achieved by perfusion of detergent-based solutions through the renal artery. (ufl.edu)
  • Can human ASCs and SVF cell implants cause tumors either directly through transformation or indirectly by promoting the growth of endogenous tumor cells? (seattlestemcellcenter.net)
  • With encapsulated cells, we are developing methods to improve immunoprotection by combining the semipermeable barrier with the local presentation and delivery of pro-survival and insulinotropic factors. (aiche.org)
  • We found that connective tissue growth factor (Ctgf) was responsible for EleS-induced CSC survival and adhesion. (elsevier.com)
  • This method is not ideal because of donor site morbidity, the requirement for additional surgery, and limited donor tissue availability. (hindawi.com)
  • Improved HPL standardization and safety profile, through larger pools and dedicated viral inactivation and removal treatments, is expected in the next few years, broadening applications in cell therapy and regenerative medicine. (elsevier.com)
  • Published definition of the cell identity with a panel of surface antigens and establishment of universally applied quality assurance/ quality control testing criteria would benefit the fledgling adipose stem cell community. (seattlestemcellcenter.net)
  • Recently, we reported the rapid fabrication technique of a double-layered cell sheet-tissue by the combination of cell sheet stacking technology and centrifugation . (thefreelibrary.com)
  • Traditionally, the process of isolating the nucleated cell fraction from the adipose tissue is based on collagenase digestion, followed by a series of centrifugation steps to isolate the specific cells. (scielo.br)
  • Cytoprotective effects and cell adhesion ability were significantly increased by EleS as evaluated by transferase-mediated dUTP nick-end labelling (TUNEL), lactate dehydrogenase (LDH) release assay, and adhesion assay. (elsevier.com)
  • Furthermore, we develop technologies for cryopreservation of encapsulated cells and for monitoring grafts in minimally invasive or non-invasive ways. (aiche.org)