The phenomenon by which dissociated cells intermixed in vitro tend to group themselves with cells of their own type.
The formation of clumps of RED BLOOD CELLS under low or non-flow conditions, resulting from the attraction forces between the red blood cells. The cells adhere to each other in rouleaux aggregates. Slight mechanical force, such as occurs in the circulation, is enough to disperse these aggregates. Stronger or weaker than normal aggregation may result from a variety of effects in the ERYTHROCYTE MEMBRANE or in BLOOD PLASMA. The degree of aggregation is affected by ERYTHROCYTE DEFORMABILITY, erythrocyte membrane sialylation, masking of negative surface charge by plasma proteins, etc. BLOOD VISCOSITY and the ERYTHROCYTE SEDIMENTATION RATE are affected by the amount of erythrocyte aggregation and are parameters used to measure the aggregation.
The internal resistance of the BLOOD to shear forces. The in vitro measure of whole blood viscosity is of limited clinical utility because it bears little relationship to the actual viscosity within the circulation, but an increase in the viscosity of circulating blood can contribute to morbidity in patients suffering from disorders such as SICKLE CELL ANEMIA and POLYCYTHEMIA.
Adherence of cells to surfaces or to other cells.
The aggregation of suspended solids into larger clumps.
The deformation and flow behavior of BLOOD and its elements i.e., PLASMA; ERYTHROCYTES; WHITE BLOOD CELLS; and BLOOD PLATELETS.
Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.
Drugs or agents which antagonize or impair any mechanism leading to blood platelet aggregation, whether during the phases of activation and shape change or following the dense-granule release reaction and stimulation of the prostaglandin-thromboxane system.
Methylmaleic anhydrides.
A genus of protozoa, formerly also considered a fungus. Its natural habitat is decaying forest leaves, where it feeds on bacteria. D. discoideum is the best-known species and is widely used in biomedical research.
Ability of ERYTHROCYTES to change shape as they pass through narrow spaces, such as the microvasculature.
Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body.
The study of the deformation and flow of matter, usually liquids or fluids, and of the plastic flow of solids. The concept covers consistency, dilatancy, liquefaction, resistance to flow, shearing, thixotrophy, and VISCOSITY.
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.
The phylum of sponges which are sessile, suspension-feeding, multicellular animals that utilize flagellated cells called choanocytes to circulate water. Most are hermaphroditic. They are probably an early evolutionary side branch that gave rise to no other group of animals. Except for about 150 freshwater species, sponges are marine animals. They are a source of ALKALOIDS; STEROLS; and other complex molecules useful in medicine and biological research.
Members of the integrin family appearing late after T-cell activation. They are a family of proteins initially identified at the surface of stimulated T-cells, but now identified on a variety of cell types. At least six VLA antigens have been identified as heterodimeric adhesion receptors consisting of a single common beta-subunit and different alpha-subunits.
Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.
Surface ligands that mediate cell-to-cell adhesion and function in the assembly and interconnection of the vertebrate nervous system. These molecules promote cell adhesion via a homophilic mechanism. These are not to be confused with NEURAL CELL ADHESION MOLECULES, now known to be expressed in a variety of tissues and cell types in addition to nervous tissue.
Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products.
The volume of packed RED BLOOD CELLS in a blood specimen. The volume is measured by centrifugation in a tube with graduated markings, or with automated blood cell counters. It is an indicator of erythrocyte status in disease. For example, ANEMIA shows a low value; POLYCYTHEMIA, a high value.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A species of motile, free-living, gram-negative bacteria that occur in the soil. They are aerobic or microaerophilic and are sometimes capable of nitrogen fixation.
An integrin heterodimer widely expressed on cells of hematopoietic origin. CD11A ANTIGEN comprises the alpha chain and the CD18 antigen (ANTIGENS, CD18) the beta chain. Lymphocyte function-associated antigen-1 is a major receptor of T-CELLS; B-CELLS; and GRANULOCYTES. It mediates the leukocyte adhesion reactions underlying cytolytic conjugate formation, helper T-cell interactions, and antibody-dependent killing by NATURAL KILLER CELLS and granulocytes. Intracellular adhesion molecule-1 has been defined as a ligand for lymphocyte function-associated antigen-1.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A division of organisms that exist vegetatively as complex mobile plasmodia, reproduce by means of spores, and have complex life cycles. They are now classed as protozoa but formerly were considered fungi.
A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.
Established cell cultures that have the potential to propagate indefinitely.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.
A group of glucose polymers made by certain bacteria. Dextrans are used therapeutically as plasma volume expanders and anticoagulants. They are also commonly used in biological experimentation and in industry for a wide variety of purposes.
The rate dynamics in chemical or physical systems.
A chelating agent that sequesters a variety of polyvalent cations such as CALCIUM. It is used in pharmaceutical manufacturing and as a food additive.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Antibodies produced by a single clone of cells.
A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A series of progressive, overlapping events, triggered by exposure of the PLATELETS to subendothelial tissue. These events include shape change, adhesiveness, aggregation, and release reactions. When carried through to completion, these events lead to the formation of a stable hemostatic plug.
Chemically stimulated aggregation of cell surface receptors, which potentiates the action of the effector cell.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
The movement of cells or organisms toward or away from a substance in response to its concentration gradient.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Proteins prepared by recombinant DNA technology.
Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.
A cell-surface ligand involved in leukocyte adhesion and inflammation. Its production is induced by gamma-interferon and it is required for neutrophil migration into inflamed tissue.
A fibrous protein complex that consists of proteins folded into a specific cross beta-pleated sheet structure. This fibrillar structure has been found as an alternative folding pattern for a variety of functional proteins. Deposits of amyloid in the form of AMYLOID PLAQUES are associated with a variety of degenerative diseases. The amyloid structure has also been found in a number of functional proteins that are unrelated to disease.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Processes involved in the formation of TERTIARY PROTEIN STRUCTURE.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
Proteins found in any species of bacterium.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
A cell line derived from cultured tumor cells.
Elements of limited time intervals, contributing to particular results or situations.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
A synuclein that is a major component of LEWY BODIES that plays a role in neurodegeneration and neuroprotection.
Duration of blood flow after skin puncture. This test is used as a measure of capillary and platelet function.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
An enzyme formed from PROTHROMBIN that converts FIBRINOGEN to FIBRIN.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
An unstable intermediate between the prostaglandin endoperoxides and thromboxane B2. The compound has a bicyclic oxaneoxetane structure. It is a potent inducer of platelet aggregation and causes vasoconstriction. It is the principal component of rabbit aorta contracting substance (RCS).
The sum of the weight of all the atoms in a molecule.
Surface glycoproteins on platelets which have a key role in hemostasis and thrombosis such as platelet adhesion and aggregation. Many of these are receptors.
Disruption of the non-covalent bonds and/or disulfide bonds responsible for maintaining the three-dimensional shape and activity of the native protein.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Methods for maintaining or growing CELLS in vitro.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Glycoproteins found on the membrane or surface of cells.
A stable, physiologically active compound formed in vivo from the prostaglandin endoperoxides. It is important in the platelet-release reaction (release of ADP and serotonin).
The assembly of the QUATERNARY PROTEIN STRUCTURE of multimeric proteins (MULTIPROTEIN COMPLEXES) from their composite PROTEIN SUBUNITS.
Laboratory examination used to monitor and evaluate platelet function in a patient's blood.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Peptides generated from AMYLOID BETA-PEPTIDES PRECURSOR. An amyloid fibrillar form of these peptides is the major component of amyloid plaques found in individuals with Alzheimer's disease and in aged individuals with trisomy 21 (DOWN SYNDROME). The peptide is found predominantly in the nervous system, but there have been reports of its presence in non-neural tissue.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.

Mechanisms of GDF-5 action during skeletal development. (1/2029)

Mutations in GDF-5, a member of the TGF-beta superfamily, result in the autosomal recessive syndromes brachypod (bp) in mice and Hunter-Thompson and Grebe-type chondrodysplasias in humans. These syndromes are all characterised by the shortening of the appendicular skeleton and loss or abnormal development of some joints. To investigate how GDF-5 controls skeletogenesis, we overexpressed GDF-5 during chick limb development using the retrovirus, RCASBP. This resulted in up to a 37.5% increase in length of the skeletal elements, which was predominantly due to an increase in the number of chondrocytes. By injecting virus at different stages of development, we show that GDF-5 can increase both the size of the early cartilage condensation and the later developing skeletal element. Using in vitro micromass cultures as a model system to study the early steps of chondrogenesis, we show that GDF-5 increases chondrogenesis in a dose-dependent manner. We did not detect changes in proliferation. However, cell suspension cultures showed that GDF-5 might act at these stages by increasing cell adhesion, a critical determinant of early chondrogenesis. In contrast, pulse labelling experiments of GDF-5-infected limbs showed that at later stages of skeletal development GDF-5 can increase proliferation of chondrocytes. Thus, here we show two mechanisms of how GDF-5 may control different stages of skeletogenesis. Finally, our data show that levels of GDF-5 expression/activity are important in controlling the size of skeletal elements and provides a possible explanation for the variation in the severity of skeletal defects resulting from mutations in GDF-5.  (+info)

Analysis of the stimulation-inhibition paradox exhibited by lymphocytes exposed to concanavalin A. (2/2029)

High doses of Concanavalin A (Con A), which normally inhibit T-lymphocyte stimulation as measured by increases in DNA synthesis, cause these lymphocytes to become committed to mitogenesis while also generating a dominant but reversible negative growth signal. The observed response to the stimulatory signal as measured by the rate of commitment to enter the S phase (i.e., the rate at which the stimulation becomes lectin independent) increases with lectin concentration even in the inhibitory range. The generation of this positive signal is prevented by treating the cells with colchicine. Cells that have become committed but are also simultaneously blocked from entering the S phase by the high doses of Con A can begin synthesizing DNA if the lectin is released by adding a competitive inhibitor of binding. Experiments done in agarose cultures in which lymphocytes are kept from contact with each other suggest that the reversible inhibitory signal is mediated by structures in the individual cells rather than as a result of agglutination. Continuously dividing cells of the lymphoid line P388 are also individually and reversibly inhibited by Con A. These findings are considered in terms of the relation of the inhibitory signal to the microtubular components of cell surface modulating assemblies made up of submembranous arrays of microtubules, microfilaments, and associated proteins.  (+info)

The pro-phenoloxidase of coleopteran insect, Tenebrio molitor, larvae was activated during cell clump/cell adhesion of insect cellular defense reactions. (3/2029)

To characterize the proteins involved in cell clump/cell adhesion of insect cellular defense reactions, we induced the cell clump/cell adhesion reaction in vitro with the hemolymph of larvae of the coleopteran insect, Tenebrio molitor. The 72 kDa protein was specifically enriched in the residues of cell clump/cell adhesion and was purified to homogeneity. A cDNA clone for the 72 kDa protein was isolated. We found that the 72 kDa protein was an activated phenoloxidase from Tenebrio pro-phenoloxidase. We suggest that activated phenoloxidase is involved in the cell clump/cell adhesion reaction as well as in the synthesis of melanin.  (+info)

Distinct functions of alpha3 and alpha(v) integrin receptors in neuronal migration and laminar organization of the cerebral cortex. (4/2029)

Changes in specific cell-cell recognition and adhesion interactions between neurons and radial glial cells regulate neuronal migration as well as the establishment of distinct layers in the developing cerebral cortex. Here, we show that alpha3beta1 integrin is necessary for neuron-glial recognition during neuronal migration and that alpha(v) integrins provide optimal levels of the basic neuron-glial adhesion needed to maintain neuronal migration on radial glial fibers. A gliophilic-to-neurophilic switch in the adhesive preference of developing cortical neurons occurs following the loss of alpha3beta1 integrin function. Furthermore, the targeted mutation of the alpha3 integrin gene results in abnormal layering of the cerebral cortex. These results suggest that alpha3beta1 and alpha(v) integrins regulate distinct aspects of neuronal migration and neuron-glial interactions during corticogenesis.  (+info)

Multiple developmental roles for CRAC, a cytosolic regulator of adenylyl cyclase. (5/2029)

Receptor-mediated activation of adenylyl cyclase (ACA) in Dictyostelium requires CRAC protein. Upon translocation to the membrane, this pleckstrin homology (PH) domain protein stimulates ACA and thereby mediates developmental aggregation. CRAC may also have roles later in development since CRAC-null cells can respond to chemotactic signals and participate in developmental aggregation when admixed with wild-type cells, but they do not complete development within such chimeras. To test whether the role of CRAC in postaggregative development is related to the activation of ACA, chemotactic aggregation was bypassed in CRAC-null cells by activating the cAMP-dependent protein kinase (PKA). While such strains formed mounds, they did not complete fruiting body morphogenesis or form spores. Expression of CRAC in the prespore cells of these strains rescued sporulation and fruiting body formation. This later function of CRAC does not appear to require its PH domain since the C-terminal portion of the protein (CRAC-DeltaPH) can substitute for full-length CRAC in promoting spore cell formation and morphogenesis. No detectable ACA activation was observed in any of the CRAC-null strains rescued by PKA activation and expression of CRAC-DeltaPH. Finally, we found that the development of CRAC-null ACA-null double mutants could be rescued by the activation of PKA together with the expression of CRAC-DeltaPH. Thus, there appears to be a required function for CRAC in postaggregative development that is independent of its previously described function in the ACA activation pathway.  (+info)

Origin of the integrin-mediated signal transduction. Functional studies with cell cultures from the sponge Suberites domuncula. (6/2029)

Sponges (phylum Porifera) represent the phylogenetically oldest metazoan animals. Recently, from the marine sponge Geodia cydonium a first cDNA encoding a putative integrin receptor molecule was isolated. In the present study basic functional experiments have been conducted to test the hypothesis that in sponges integrin polypeptides also function as adhesion molecules and as outside-in signaling molecules. The sponge Suberites domuncula has been used for the experiments because from this sponge only has a cell culture been established. Here we report that aggregation factor (AF)-mediated cell-cell adhesion is blocked by the RGDS peptide which is known to interact with beta integrin. Both RGDS and AF were found to stimulate DNA synthesis within 24 h. The beta subunit of the integrin receptor was cloned from S. domuncula; the estimated 91-kDa molecule comprises the characteristic signatures. Evolutionary conservation of the beta integrin was assessed by comparison with corresponding beta integrin subunits from evolutionary higher metazoan taxa. Addition of RGDS or of AF to isolated cells of S. domuncula causes a rapid (within 1-2 min) increase in the intracellular Ca2+ concentration which is further augmented in the presence of Ca2+. Furthermore, incubation of the cells with RGDS or AF causes an activation of the GTP-binding protein Ras. In addition it is shown that after a prolonged incubation of the cells with RGDS and AF the expression of the genes coding for Ras and for calmodulin is upregulated. These results suggest that the integrin receptor functions in the sponge system not only as adhesion molecule but also as a molecule involved in outside-in signaling.  (+info)

E-cadherin mediates aggregation-dependent survival of prostate and mammary epithelial cells through the retinoblastoma cell cycle control pathway. (7/2029)

E-cadherin and the retinoblastoma tumor suppressor (Rb) are traditionally associated with diverse regulatory aspects of cell growth and differentiation. However, we have discovered new evidence, which suggests that these proteins are functionally linked in a physiologic pathway required for cell survival and programmed cell death. Pharmacological activation of protein kinase C (PKC) or inducible overexpression and activation of the alpha isozyme of PKC (PKCalpha) resulted in approximately 60% apoptosis of mammary and prostate epithelial cells. Interestingly, the surviving cells had undergone dramatic aggregation concurrent with increased E-cadherin expression. When aggregation was inhibited by the addition of an E-cadherin-blocking antibody, apoptosis increased synergistically. We hypothesized that survival of the aggregated population was associated with contact-inhibited growth and that apoptosis might result from aberrant growth regulatory signals in non-aggregated, cycling cells. This hypothesis was confirmed by experiments that demonstrated that E-cadherin-dependent aggregation resulted in Rb-mediated G1 arrest and survival. Immunoblot analysis and flow cytometry revealed that hypophosphorylated Rb was present in non-aggregated, S phase cultures concurrent with synergistic cell death. We have also determined that the loss of membrane E-cadherin and subsequent hypophosphorylation of Rb in luminal epithelial cells preceded apoptosis induced by castration. These findings provide compelling evidence that suggests that E-cadherin-mediated aggregation results in Rb activation and G1 arrest that is critical for survival of prostate and mammary epithelial cells. These data also indicate that Rb can initiate a fatal growth signal conflict in non-aggregated, cycling cells when the protein is hypophosphorylated as these epithelial cells enter S phase.  (+info)

Differential responses to CD40 ligation among Burkitt lymphoma lines that are uniformly responsive to Epstein-Barr virus latent membrane protein 1. (8/2029)

Ligation of CD40 on the surface of B cells induces multiple phenotypic effects, many of which are mimicked by the EBV latent membrane protein 1 (LMP1) through its interaction with downstream components of the CD40 signaling pathway. Because the effects of LMP1 have been most closely studied in human Burkitt Lymphoma (BL) cell lines retaining a tumor biopsy-like phenotype in vitro, we have examined the response of a panel of such lines to CD40 ligation. Two distinct patterns of response were observed that were unrelated to the surface level of CD40 or to the EBV genome status of the lines. Following exposure to either CD40-specific mAbs or the soluble trimeric ligand (sCD40L), high responder (HR) lines showed rapid aggregation, activation of NF-kappa B, up-regulation of cell surface markers ICAM-1/CD54 and Fas/CD95, and growth inhibition. Aggregation was seen at lower doses than those required to elicit the other effects. By contrast, low responder (LR) lines showed no detectable response to CD40 mAbs, while their responses to sCD40L were limited to activation of NF-kappa B and up-regulation of CD95 only. However, in transfection experiments, LMP1 uniformly induced the full spectrum of phenotypic effects in both HR and LR lines. We conclude that some BL cell lines show a highly restricted response to CD40 ligation but remain fully susceptible to LMP1.  (+info)

We study spreading on soft substrates of cellular aggregates using CT26 cells that produce an extracellular matrix (ECM). Compared to our previous work on the spreading of S180 cellular aggregates, which did not secrete ECMs, we found that the spreading velocity of the precursor film is also maximal for inte
We had one patient that asked the phlebotomist to come in the hospital to take their blood cause they got. [inaudible 00:44:24] I wasnt, we couldnt do that, but it was. So this is looking at the cellular aggregates and what we noticed, this is consistent with what weve seen in the animal models as well as what weve seen in the PDE9 study, that when a patient is reporting a VOC, we actually see an increase in cellular aggregates in terms of the monocyte platelet aggregates as well as the neutrophil platelet aggregates when you look at baseline comparison. In addition, we did see some changes in adhesion to P-selectin under flow conditions shown in the bottom. So both of these we do see changes in aggregates when a patient is self-reporting a VOC. So in addition to the other biomarkers that we said, we also took clinical laboratories and this is looking at the standard hemoglobin, hemoglobin F, nucleated red blood cells, eosinophiles as well as C-reactive protein. Interestingly, eosinophiles ...
Clone DB105 recognizes the CD44 antigen. CD44 is a marker for many types of cancer stem cells (CSCs), including breast CSCs that possess higher tumorigenicity and metastatic potential, colorectal, pancreatic, and prostate CSCs. In addition, expression was observed in several cancers as well as on carcinoma cell lines. Here, CD44 plays a role in cancer cell migration and matrix adhesion in response to a cellular microenvironment, thus enhancing cellular aggregation and tumor cell growth. CD44 is also expressed on mesodermal cells, such as hematopoietic, fibroblastic, and glial cells. - Österreich
TY - GEN. T1 - Role of cell aggregation protein in adhesion in vitro and in vivo. AU - Morelli, Lorenzo. AU - Cesena, Camilla. AU - Lucchini, Franco. AU - Callegari, Maria Luisa. AU - Alander, Minna. AU - Mattila-Sandholm, Tiina. AU - Wright, Atte von. AU - Salminen, Seppo. AU - Lehto, Elina. AU - Vilpponen-Salmela, Terttu. N1 - Poster. PY - 1997. Y1 - 1997. M3 - Conference article in proceedings. SN - 951-38-4564-8. T3 - VTT Symposium. SP - 63. EP - 64. BT - Novel methods for probiotic research. PB - VTT Technical Research Centre of Finland. CY - Espoo. ER - ...
Scientists estimate that planet Earth formed about 4.5 billion years ago as part of the formation of the solar system. It was only about one billion years later that the first unicellular organisms made their debut, and it took about two billion years more for multicellular organisms to appear. Initially these were simple cellular aggregates that later on evolved into more complex life forms with distinct tissues and organs. The most dramatic segment of evolution took place about a half billion years ago when over a "brief” period of 30 million years the foundations were laid for all the groups of animals that still surround us today. The following video describes the basics of how life is believed to have emerged and evolved on Earth, from the formation of DNA to the appearance of human beings. It should be mentioned that the video contains several inaccuracies on which I will elaborate in the following paragraphs.
A rotating cell culture system that allows epithelial cells to grow under physiological conditions resulting in 3-D cellular aggregate...
Caring Medical discusses treatments for Barré-Liéou and Cervicocranial syndrome due to vertebral instability, which affects the function of the nerve cell aggregations located in the neck just in front of the vertebrae.
Report prepared at Wed, 13 Dec 2017 00:09:45 UTC+1000, using data obtained within AS0.6447. The report may include routes internal to AS0.6447, and may also include routes that are accepted from adjacent ASs and marked NO EXPORT. The report also does not take into account conditions local to each origin AS in terms of policy or traffic engineering requirements. As an aggregation guide, this report is a very approximate guide at best.. ...
metrics aggregation that counts the number of values that are extracted from the aggregated documents. These values can be extracted either from specific fields in the documents, or be generated by a provided script. Typically, this aggregator will be used in conjunction with other single-value aggregations. For example, when computing the ...
Aggregations (Group-by, Pivot-table, and N-dimensional Cube) and Time Series Transformations as Stored Procedures in DocumentDB - lmaccherone/documentdb-lumenize
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Hello we have the following unique algo aa,a,nd we would like to know if it could be interesting in Bio. Does somebody can help ...
Campaigning in Florida, President Barack Obama told supporters that his Republican challenger, Mitt Romney, doesnt represent change in the 2012 election. Obama told them, you know what I mean, and I mean what I say. (Nov. 4)
Exposure of Sulfolobus solfataricus to the DNA damaging agents UV-irradiation, bleomycin or mitomycin C induces cellular aggregation.[2] Other physical stressors, such as pH or temperature shift, do not induce aggregation, suggesting that induction of aggregation is caused specifically by DNA damage. Ajon et al.[3] showed that UV-induced cellular aggregation mediates chromosomal marker exchange with high frequency. Recombination rates exceeded those of uninduced cultures by up to three orders of magnitude. Frols et al.[2][4] and Ajon et al.[3] hypothesized that the UV-inducible DNA transfer process and subsequent homologous recombinational repair represents an important mechanism to maintain chromosome integrity. This response may be a primitive form of sexual interaction, similar to the more well-studied bacterial transformation that is also associated with DNA transfer between cells leading to homologous recombinational repair of DNA damage.[5] In another related species, Sulfolobus ...
Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541 ...
The species-specific cell adhesion in the marine sponge Microciona prolifera involves the interaction of an extracellular proteoglycan-like macromolecular complex, otherwise known as aggregation factor. In the interaction, two highly polyvalent functional domains play a role: a cell-binding and a self-interaction domain. The self-recognition has been characterized as a Ca2+-dependent carbohydrate-carbohydrate ... read more interaction of repetitive low affinity carbohydrate epitopes. One of the involved epitopes is the pyruvated trisaccharide b-D-Galp4,6(R)Pyr-(1→4)-b-D-GlcpNAc-(1→3)-LFucp. To evaluate the role of this trisaccharide in the proteoglycan-proteoglycan self-recognition, b-D-Galp4,6(R)Pyr-(1→4)-b-D-GlcpNAc-(1→3)-a-L-Fucp-(1→O)(CH2)3S(CH2)6SH was synthesized, and partially converted into gold glyconanoparticles. These mimics are being used to explore the self-interaction phenomenon for the trisaccharide epitope, via TEM aggregation experiments (gold glyconanoparticles) and ...
Using freeze-fracture electron microscopy and fluorescent dye injection we have analysed the contacts between cells of the deeper endoderm taken from neuru
The fixation of dissociated chick embryonic cells in the presence of ruthenium red or alcian blue revealed a remarkable reduction in the binding of these cations to cell surfaces following tryptic digestion at 37 °C, but not after trypsin used at 4 °C or EDTA.. The reconstitution of anionic sites present over the cell plasma membrane was studied in retina, liver and heart cells from embryos of different ages, cultivated according to a method which promotes cell reaggregation. All these cells regenerated ruthenium red-stainability within the first hour of culture. Culturing at 15 °C, substitution of saline for the culture medium or addition of puromycin or of neuraminidase to the culture media did not affect this restitutive process. Neuraminidase and hyaluronidase digestion were not able to alter the ruthenium red-binding, once it was present.. From these data and from the reports available in the literature it has been concluded that the anionic binding-sites cannot be identified with the ...
Buy PEAR1 elisa kit, Mouse platelet endothelial aggregation receptor 1 ELISA Kit-NP_001027585.1 (MBS9332849) product datasheet at MyBioSource, ELISA Kits
Demonstration of the cellular aggregation which constitutes the lupus erythematosus (LE) phenomenon in heparinized bone marrow1 was the basis of the first in a series of specific diagnostic procedures in systemic lupus erythematosus (SLE).2 When this phenomenon was also demonstrated in heparinized3 and in clotted4 peripheral blood of patients suffering from this disease, such preparations were also used in laboratory diagnosis. Finally, the reproduction in vitro of the LE phenomenon by adding LE plasma or serum to cells of normal bone marrow5, 6 added another in this series of tests.. Thus, the three types of diagnostic procedures used have in ...
Cloning of a heat-inducible biomarker, the cDNA encoding the 70 kDa heat shock protein, from the marine sponge Geodia cydonium:response to natural ...
Related Transcriptional Enhancer Factor-1 (RTEF-1) has been suggested to induce angiogenesis through regulating target genes. Whether RTEF-1 has a direct role in angiogenesis and what specific genes are involved in RTEF-1 driven angiogenisis have not been elucidated. We found that over-expressing RTEF-1 in Human dermal microvascular endothelial cells-1 (HMEC-1) significantly increased endothelial cell aggregation, growth and migration while the processes were inhibited by siRNA of RTEF-1. In addition, we observed that Endothelial differentiation gene-1 (Edg-1) expression was up-regulated by RTEF-1 at the transcriptional level. RTEF-1 could bind to Edg-1 promoter and subsequently induce its activity. Edg-1 siRNA significantly blocked RTEF-1-driven increases in endothelial cell aggregation in a Matrigel assay and retarded RTEF-1-induced endothelial cell growth and migration. Pertussis Toxin (PTX), a Gi/Go protein sensitive inhibitor, was found to inhibit RTEF-1 driven endothelial cell aggregation ...
The reaggregation of dissociated cells to form organotypic structures provides an in vitro system for the analysis of the cellular interactions and molecular mechanisms involved in the formation of tissue architecture. The retina, an outgrowth of the forebrain, is a precisely layered neural tissue, yet the mechanisms underlying layer formation are largely unexplored. Here we describe the protocol to dissociate, re-aggregate, and culture zebrafish retinal cells from a transgenic, Spectrum of Fates, line where all main cell types are labelled with a combination of fluorescent proteins driven by fate-specific promoters. These cells re-aggregate and self-organize in just 48 h in minimal culture conditions. We also describe how the patterning in these aggregates can be analyzed using isocontour profiling to compare whether different conditions affect their self-organization ...
Mechanistically, IgSF9 and IgSF9b share similarities, but also seem to have distinct properties. Both CAMs mediate homophilic adhesion or homotypic aggregation and are not sufficient to induce synapse formation. Both proteins are localized at inhibitory synapses. Interestingly, IgSF9b was shown to act on the postsynaptic side of inhibitory synapses onto interneurons (Woo et al., 2013), whereas our study suggests that IgSF9 is enriched on the presynaptic side and is required for inhibitory synapse onto pyramidal neurons. Whether IgSF9 and IgSF9b are necessary in either the presynaptic or postsynaptic compartment in vivo will require conditional knock-out mice and is a direction of future studies.. The most striking difference between IgSF9 and IgSF9b was the role of their respective intracellular domains (ICDs). The ICD of IgSF9b was shown to interact with Neuroligin-2 by way of the scaffolding protein S-SCAM and it was suggested, although not formally shown, that this interaction is necessary ...
The researchers took on one of the most insidious problems, compound aggregation, in which small molecules form colloids that bind to and partially denature proteins, causing false positives in all sorts of assays. This can happen even at nanomolar concentrations of compound, and is all the more problematic at higher concentrations used in fragment screening and early hit to lead optimization. In many cases aggregates can be disrupted or passivated by including nonionic detergents such as Triton X-100 or Tween-80, but not all assays tolerate detergent, and some aggregates form even in the presence of detergent ...
1. As a preliminary study on the molecular basis of cell aggregation, the soluble macromolecular fractions of growing (vegetative) and aggregation competent cells of D. discoideum were compared. a) ...
Nam, M. H., Dewey D. Y. Ryu, B. R. Thomas, and R. Rodriguez. 1997. Expression and secretion of fusion protein (GUS) in the suspension culture of transgenic 3DG rice cell line: Effects of osmotic pressure, buffering capacity of pH and cell aggregation on GUS expression. J. Crop Science, 39: 9 -14 ...
Home › A disaggregated biodiversity offset accounting model to improve estimation of ecological equivalency and no net loss ...
We specialize in using statistical and soft matter physics methods and advanced computational tools to understand stabilities and dynamic transitions of various molecular and cellular biosystems, from single biomolecules, assemblies, to cell dynamics. We are particularly interested in mechanical structures at the nanometer to micrometer scale. Our research spans from internal motions of biomolecules (often near equilibrium) to subcellular assemblies and cell aggregation, which can be far-from-equilibrium systems. These nanoscale molecular machines are not only quite complicated structurally, but have evolved intriguing functions responding to many environmental pressures. It is both fun and important to answer questions such as: How stable and robust are their functions? Under what conditions will they fail? How quickly can such molecules carry out their functions? How does a molecule actually pick out one unique, or maybe a few conformations, from an astronomical number of possibilities? How ...
In mid-1998 the 191 distributor Hgh molecule was genetically engineered in the workplace. Since then all HGH is synthetically produced and HGH from cadavers is no mortal in use. Inductive HGH (Somatrophin) is free to us now in two forms. One is through a pricey doctors pharmaceutical prescription, and secondly at a valid toll through a homeopathic exam spray. A position therapy deciding would be Secretogogues and Stacked Radical products which take absolutely no HGH. The aim here is to cable your own body, by stimulating the Endocrine Secretor, to food many of its own HGH. Alas this latter therapy totality for only a month or two ...
UfiSpace, the leading provider of open disaggregated networking solutions, has announced the availability of their S9600 series open aggregation routers, which are built on Broadcoms Qumran2c silicon. Their open aggregation routers come in three models with various port configurations and capab...
You can filter specific content in the table using the column filters. Click the filter of the desired column and write the desired text. The filter icon will turn green and the table will show only those aggregation tasks containing that string in that column. This method can be used to filter by Name and Owner.. You can also filter by clicking cells on the table, or a specific part of it (for example, a tag or a status icon), to use its content as filtering criteria. The table will only show those aggregation tasks containing that information. This method can be used to filter by Status, Owner and Tag.. ...
Serialization solution shown by OCS Checkweigher focuses on aggregation of bottles in multilayer cases. Aggregation is finalized when cases go onto a pallet.
PV Economics, Markets and Policies, PV Economics and Markets, Development of Innovative Self-Consumption and Aggregation Concepts for PV Prosumers to Improve Grid Load and Increase Market Value of PV: The PV-Prosumers4Grid Project
The fine aggregate is natural sand that has been washed and sieved to remove particles larger than 5 mm, and the coarse aggregate is a gravel that has been crushed, washed and sieved so that the particles vary from 5 to 50 mm in size.The fine and coarse aggregate is delivered separately. As they need to be sieved, a prepared mixture of fine and coarse aggregate is more expensive than the .... ...
Page contains details about BiPy-BTA discotic 1 aggregates . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
So when it comes to applying sizing installing and supporting your equipment needs think of Aggregate Crusher Specialists as your first call for support Proudly Representing Sandvik Crushing amp Screening
Swedish University dissertations (essays) about HäLSA. Search and download thousands of Swedish university dissertations. Full text. Free.
TY - JOUR. T1 - A study of whole blood platelet and white cell aggregation using a laser flow aggregometer. AU - Sun, J.. AU - Abel, E. W.. AU - Bancroft, A.. AU - McLaren, M.. AU - Belch, J. J. F.. PY - 2003. Y1 - 2003. N2 - Both platelet aggregation and white blood cell aggregation are involved in pathological processes such as thrombosis, atherosclerosis and chronic inflammation. People in older age groups are likely to suffer from cardiovascular diseases and may have increased white cell and platelet aggregation which could contribute to this increased risk. This study aimed to compare white cell and platelet aggregation between different age and gender groups. Whole blood white cell aggregation and platelet aggregation were carried out on healthy volunteers using cytometric techniques. It was found that both white cell and platelet aggregation in the elderly group (white cell aggregation median value, 0.08; range, 0.02-0.14; platelet aggregation median value, 0.32; range, 0.1-0.39) were ...
Membrane interaction in aggregating cells of Dictyostelium discoideum can be blocked by univalent antibodies directed against specific membrane sites. Using a quantitative technique for measuring cell association, two classes of target sites for blocking antibodies were distinguished and their developmental dynamics studied. One class of these sites is specific for aggregation-competent cells, their quantity rising from virtually 0-level during growth, with a steep increase shortly before cell aggregation. The serological activity of these structures is species specific; they are not detectable in a nonaggregating mutant, but present in a revertant undergoing normal morphogenesis. Patterns of cell assembly in the presence of antibodies show that selective blockage of these membrane sites abolishes the preference for end-to-end association which is typical for aggregating cells. A second class of target sites is present in comparable quantities in particle fractions from both growth-phase and ...
The aggregation phenomenon in lactococci has been studied for more than thirty years. It was found to be directly associated with a sex factor and lactose plasmid co-integration event [1] or duplication of the cell wall spanning (CWS) domain of PrtP proteinase [2]. Lactose plasmid conjugation in Lactococcus lactis 712 and in the related strains C2 and ML3, frequently involves plasmid co-integration with a sex factor. Moreover, this phenomenon is often associated with a cell aggregation phenotype and high frequency transfer ability [3-5]. The lactococcal sex factor exists integrated in the chromosome [6], although it can be excised as a closed circular form and lost from the cell [1]. Deletion and over-expression experiments confirmed that CluA is the only sex factor component responsible for aggregation in L. lactis. This 136 kDa surface-bound protein, encoded by the chromosomally located sex factor of Lactococcus lactis subsp. cremoris MG1363, is associated with cell aggregation linked to ...
Abstract. We have recently found that antibodies to L-selectin, the homing receptor on neutrophils, are as effective as those to beta 2-integrin at blocking fo
The purpose of these studies was to determine whether hematogenous clonal pulmonary melanoma metastases originate from the expansion of a single cell and if so, by extrapolation, metastasis can be considered a cloning process. Three different cell lines of murine K-1735 melanoma with different metastatic properties and unique karyotypes were injected i.v. into syngeneic C3H/HeN mice as multicell aggregates of individual cell lines or combinations of cell lines. Resultant solitary lung metastases were isolated in culture as individual lines and then karyotyped. Even when heterogeneous clumps of tumor cells were injected, the individual metastases exhibited a karyotype unique to one metastatic cell type. Furthermore, when cellular aggregates were composed of metastatic cells admixed with cells that were tumorigenic but nonmetastatic, the resultant metastases exhibited only the karyotype of the metastatic cells. This finding suggests that the presence of metastatic cells did not change the ...
The study clearly showed that the gene correction procedures did not impair the full pluripotentiality of iPS cells, said Shinya Yamanaka (director of the Center for iPS Cell Research and Application at Kyoto University). Yamanaka is the father of the iPS field. In 2006 his laboratory was the first to coax adult mouse fibroblasts into an undifferentiated state through the expression of 4 transcription factors: Oct4, Sox2, Klf4, and c-Myc.4. Yamanaka said that the findings of the German group indicate a possibility that human iPS cells generated from people with genetic defects may be repaired and have the similar abilities. The work should encourage researchers to improve differentiation methods from iPS cells into functional cells.. An intriguing aspect of the study was the relatively high efficiency at which the groups approach yielded full-term surviving pups. Up to 60% of cellular aggregates from tetraploid complementation generated surviving pups, and a significant number of the pups ...
Because sponges (Porifera) are generally the dominant group of multicellular animals found in the marine benthos they are key animals for the testing of biomarkers in this biotope. Previously, it was shown that the heat shock protein hsp70 from the marine sponge Geodia cydonium is a useful biomarker for following biological responses to selected physical and chemical stressors. One protein which is critical for hsp70 function in other organisms is the DnaJ-like protein. In this study we therefore isolated and characterized a cDNA encoding the DnaJ-like protein from G. cydonium designated GCDNAJ. GCDNAJ is 1476 base pairs (bp) long with a 1236 bp open reading frame encoding a protein of 45542 daltons. Northern blot analysis revealed a transcript size of 1.5 kilobase (kb). The deduced amino acid (aa) sequence of GCDNAJ contains motifs characteristic of most prokaryotic and eukaryotic DnaJ family members. In response to a 7°C temperature increase, expression of the transcripts encoding hsp70 and ...
To examine if cadherin function is essential for the activity-induced spine enlargement, we took advantage of the W2A mutant of N-cadherin (W2A-cadherin), a point mutant that lacks adhesive activity (Tamura et al., 1998). The overexpression of W2A-cadherin suppressed the adhesive function of N-cadherin as examined by cell aggregation assays (Fig. 7 A). The number of dendritic protrusions on the neurons overexpressing W2A-cadherin was equal to the spine number on the control neurons (Table II). 16% of these spines on the W2A-transfected neurons remained in normal cotyloid shape (Fig. 7 C-1; Table I). 32% of spines appeared largely normal with their heads still identifiable as the bulging end, but their apexes were flat without cotyloid curve (Fig. 7 C-2; Table I). This type of spine was occasionally accompanied by transient protrusions on its apex (see Fig. 8 D). 13% showed normally bulged spine head, but with elongated neck (Fig. 7 C-3; Table I). The other 39% showed filopodia-like elongated ...
Many types of mammalian cells can aggregate and differentiate into 3-D multicellular spheroids when cultured in suspension or a nonadhesive environment. Compared to conventional monolayer cultures, multicellular spheroids resemble real tissues better in terms of structural and functional properties. Multicellular spheroids formed by transformed cells are widely used as avascular tumor models for metastasis and invasion research and for therapeutic screening. Many primary or progenitor cells on the other hand, show significantly enhanced viability and functional performance when grown as spheroids. Multicellular spheroids in this aspect are ideal building units for tissue reconstruction. Here we review the current understanding of multicellular spheroid formation mechanisms, their biomedical applications, and recent advances in spheroid culture, manipulation, and analysis techniques. ...
In aggregation of red blood cells (RBCs) induced by macromolecules, the aggregating energy provided by macromolecular binding to RBC membrane must overcome the disaggregation energy of electrostatic repulsion between RBC surfaces and the effects of m
In this paper we report a novel effect of soluble Fbg that contributes to the sequential activation of circulating neutrophils. Although β2 integrins on myeloid cells were initially identified as cell surface structures mediating the traffic and localization of circulating immune cells (37), they are also able to regulate multiple cellular responses to environmental stimuli, leading to growth, differentiation, gene expression, and apoptosis (38). We now demonstrate that soluble hFbg is capable of triggering neutrophil activation through a CD11b-dependent mechanism. It is well known that several products of coagulation and fibrinolysis profoundly affect many facets of inflammatory reactions, such as induction of platelet activation (39), cytokine production by mononuclear phagocytes (40, 41, 42), neutrophil aggregation, and chemotaxis (43, 44, 45). However, the effects of soluble hFbg on inflammatory cells have only recently been investigated. In this regard, Sitrin et al. (9) recently reported ...
The aggregation state of sickle cells was studied as a function of oxygen partial pressure, with ultrasound backscattering. The ultrasonic backscattering coefficient χ , which depends on the size of the aggregates, is highly correlated with erythrocy
In 1975, Orci et al. (1) reported that human islet cells contain specialized membrane domains that are compatible with the ultrastructural features of two types of intercellular junctions: tight junctions and gap junctions. Since then, numerous reports have demonstrated critical functions for these cell-cell junctional complexes in islet cells (2-8). Eventually, a number of proteins were identified that regulated cell aggregation, islet cell-type segregation, architectural organization within islets of Langerhans, and state of differentiation, cell growth, and hormone secretion (9-16). Hints that direct islet cell-to-islet cell interactions are required for proper insulin secretion were uncovered in the 1980s when it was observed that single (isolated) β-cells are unresponsive to glucose unless they are given the opportunity to reaggregate into small clusters (17). Even more interesting, it was observed that islet cell types harbor specific cell-to-cell recognition signatures that drive their ...
We have recently demonstrated that the CD40 molecule was expressed on both normal human plasma cells and most malignant plasma cells, i.e., myeloma cells. Thus, we have investigated its putative role in the proliferation of myeloma cells. We report that 7 of 15 myeloma cell lines were CD40+ but only one, XG2, presented a high level of CD40 expression. We show that the CD40 stimulation by anti-CD40 monoclonal antibodies (mAbs) of the interleukin 6-dependent myeloma cell line XG2 induced a total inhibition of its proliferation. This inhibition was also observed when cells were either cultured in the CD40 system, where the anti-CD40 mAb has been immobilized on fibroblasts expressing Fc receptors or in the presence of a soluble chimeric CD40 ligand molecule. This inhibition of proliferation was neither accompanied by differentiation nor apoptosis. Triggering CD40 induced an homotypic aggregation of XG2 cells, and the inhibition of proliferation was totally prevented by a blocking anti-CD18 mAb. ...
It is an interesting time to consider the means and roles of cell adhesion in the eukaryotes. There was initial wonder at the results of Wilsons reports of species-specific reaggregation of sponge...
Professor J.P. Trinkaus with Tommy Harris, Oxford University, 1987 Malcolm Steinberg (1963, and later) proposed a thermodynamic theory that sorting out of dissociated chick, mouse and amphibian cells is caused by quantitative differences in amount of cell-cell adhesiveness (amount rather than kind). The Differential Adhesion Hypothesis or DAH.. Steinberg, M.S. (1963). Reconstruction of Tissues by Dissociated Cells. Some morphogenetic tissue movements and the sorting out of embryonic cells may have a common explanation. Science 141, 401-408. [doi: 10.1126/science.141.3579.401. PMID: 13983728]. Steinberg, M.S. (1970) Does differential adhesion govern self-assembly processes in histogenesis? Equilibrium configurations and the emergence of a hierarchy among populations of embryonic cells. J Exp Zool. 173(4):395-433. [doi: 10.1002/jez.1401730406. PMID: 5429514] This theory had the great advantage of making testable predictions. Relative interior-exterior positions of sorting out should form a ...
Downloadable! In this paper we analyze judgement aggregation problems in which a group of agents independently votes on a set of complex propositions that has some interdependency constraint between them (e.g., transitivity when describing preferences). We consider the issue of judgement aggregation from the perspective of approximation. That is, we generalize the previous results by studying approximate judgement aggregation. We relax the main two constraints assumed in the current literature, Consistency and Independence and consider mechanisms that only approximately satisfy these constraints, that is, satisfy them up to a small portion of the inputs. The main question we raise is whether the relaxation of these notions significantly alters the class of satisfying aggregation mechanisms. The recent works for preference aggregation of Kalai, Mossel, and Keller fit into this framework. The main result of this paper is that, as in the case of preference aggregation, in the case of a subclass of a
Using Rezais cells, Norriss technology and Seagles leg work, the team was able to capture convincing and dramatic evidence that melanin protects the retinal cells. In the PNAS paper (21 June 2005), they show that increased melanin aggregation and radical migration within melanin aggregates can protect RPE cells from free-radical damage and help prevent cell death. In the JACS paper (17 August 2005, but available online) they demonstrate how melanin actually scavenges the harmful free radicals produced by high-energy blue or ultraviolet light as it flows into the eye, soaking them up and neutralizing their effects ...
Amphiphilic molecules have a hydrophilic head and a hydrophobic chain. Under certain conditions, they can self-organize into hollow spherical hollow vesicles with a surfactant bilayer enclosing an aqueous core, the overall diameter ranging from a few tens of nanometers to several microns. By carefully choosing specific preparation conditions, the scientists of SIS2M have shown that extremely robust aggregates can be synthesized with an interesting potential for encapsulation. The vesicles are widely studied for many fundamental issues (mechanism of self-assembly, physical properties of the membrane, etc ...) which understanding may open new perspectives (controlled release of active molecules, chemical nanoreactors, energy conversion, etc...). Usually, these vesicles (in this case also known as
Numerous bacterial strains produce surface lectins, commonly in the form of fimbriae that are filamentous assemblies of protein subunits. Among the best characterized of these are the type 1 (mannose specific) fimbrial lectins of Escherichia coli that consist almost exclusively of one class of subun …
Organizations which change service provider but do not renumber. This has the effect of punching a hole in the aggregation of the original service providers advertisement. This plan will handle the situation by requiring the newer service provider to advertise a specific advertisement for the new client, which is preferred by virtue of being the longest match. To maintain efficiency of aggregation, it is recommended that organizations which do change service providers plan to eventually migrate their address assignments from the old providers space to that of the new provider. To this end, it is recommended that mechanisms to facilitate such migration, including improved protocols and procedures for dynamic host address assignment, be developed. Note that some aggregation efficiency gain can still be had for multi-homed sites (and, in general, for any site composed of multiple, logical IP network numbers) - by allocating a contiguous power-of-two block of network numbers to the client (as ...
This research study is thus designed to compute total factor productivity (TFP) growth for Pakistans large scale manufacturing (LSM) sector for each five year period from 1970-71 to 2005-06.
Relative GDP shares are frequently used as weights in aggregations. In order to ensure that these weights reflect countries shares in real output, GDP data in national currencies should be converted into a common numeraire currency at purchasing power parity (PPP) rates. A review of the empirical evidence on the relationship between exchange rates and prices suggests that market (or official) exchange rates are generally poor proxies for PPP rates. The paper examines the PPP-based GDP data generated by the International Comparison Program and compares aggregations with PPP- and exchange rate-based GDP weights.
ETTelecom.com brings latest carrier aggregation support news, views and updates from all top sources for the Indian Telecom industry.
Aggregation Klausenpass Passroad Switzerland: 1 assigned downloads, like The Stäubifall waterfall and an aggregation of alp huts, seen from the Klausenpass road, Schächental, Uri, Switzerland from stock-photos
A mill is a device that breaks solid materials into smaller pieces by grinding, crushing, or cuttingFor instance rock crushing or grinding to produce uniform aggregate size for construction purposes, or separation of rock, soil or aggregate material for the . Rod mills are less common than ball mills for grinding minerals.. ...
Self‐incompatibility (SI) is a genetically controlled cell-cell recognition system that acts as a barrier to self pollination in a wide range of flowering plant species
We begin this discussion with an analysis of the hierarchical framework discussed in Hax and Meal, 1975. After defining the different levels of the hierarchy we provide necessary and detailed models. Several interpretations and consequences of these results are discussed. In addition, it is shown that planning horizons to insure consistency on a detailed level are intimately related to planning on an aggregate level. Although we cannot demonstrate the optimality of the approach of Hax and Meal, lower bounds are easily derived. For the remainder of the report, we do not consider the aggregate product structure of Hax and Meal. Instead, in a linear model, we aggregate all items together. Under a quite general cost structure, we are able to characterize optimal aggregate production which can be optimally disaggregated. The analysis is extended from the single to the multi-echelon case. We formalize some of the preceding notions and present a more general underlying theory. The aggregate
The AccuSizer FX Nano SIS system is specifically designed to measure protein aggregation. The instrument meets and/or exceeds all requirements described in USP
A method for performing Carrier Aggregation (CA) using spectrum shared between an incumbent user and a telecommunications network includes establishing a primary cell (PCell) for the secondary user in
The problem of finding the number and size distribution of cell clusters that grow in an organ or tissue from observations of the number and sizes of transections of such cell clusters in a planar section is considered. This problem is closely relate
Importance of an aggregate crushing value acv test greece. The aggregate crushing value ACV of an aggregate is the mass of material expressed as a percentage of the test sample which is crushed finer than a 236 mm sieve when a sample of aggregate passing the 132 mm and retained on the 950 mm sieve is subjected to crushing under a gradually applied pressive load of 400 kN 2 APPARATUS 21.. ...
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Researchers believe they figured out how Parkinsons advances by triggering a process that culminated with a Parkinsons-type disease in lab mice.
"Platelet phagocytosis and aggregation". The Journal of Cell Biology. 27 (3): 531-43. doi:10.1083/jcb.27.3.531. PMC 2106759. ... Berridge, Michael J. (1 October 2014). "Module 11: Cell Stress, Inflammatory Responses and Cell Death" (PDF). Cell Signalling ... "Programmed anuclear cell death delimits platelet life span". Cell. 128 (6): 1173-86. doi:10.1016/j.cell.2007.01.037. PMID ... Endothelial cells are attached to the subendothelial collagen by von Willebrand factor (VWF), which these cells produce. VWF is ...
In animal cells, virus particles are gathered by the microtubule-dependent aggregation of toxic or misfolded protein near the ... Viroplasms also control release of virions when the insect stings an infected plant cell or a cell near the infected cells. ... Understanding the phenomena of virus aggregation and of the cell response to the presence of virus, and whether viroplasms ... Kopito RR (December 2000). "Aggresomes, inclusion bodies and protein aggregation". Trends in Cell Biology. 10 (12): 524-30. doi ...
Bicher, Haim (1972). Blood Cell Aggregation in Thrombotic Processes. Springfield: Charles C. Thomas. ISBN 9780398022327. " ...
Protein aggregation Protein folding Live cell imaging Jennifer Lippincott-Schwartz : Intracellular Fluorescent Imaging: An ... "Live Cell Imaging". Live cell imaging enables in vivo tracking of proteins in space and time, in their natural endogenous ... which can then be followed inside a cell using a fluorescence microscope. The cell may then be treated by a perturbation of ... "Live-Cell Imaging". Lippincott-Schwartz, J.; Patterson, GH (2003). "Development and Use of Fluorescent Protein Markers in ...
"Strong aggregation and increased toxicity of polyleucine over polyglutamine stretches in mammalian cells". Human Molecular ... They may play a structural role in proteins such as collagens, myosin, keratins, silk, cell wall proteins. Tandem repeats of ... They thus hypothesize and propose that, in a cell-free environment, the early genetic code may have also produced low ... Thus, their presence may disturb the balance of protein-protein interaction networks within the cell, especially if the carrier ...
"Ataxin-1 nuclear localization and aggregation: role in polyglutamine-induced disease in SCA1 transgenic mice". Cell. 95 (1): 41 ... Cell. 127 (7): 1335-47. doi:10.1016/j.cell.2006.11.038. PMID 17190598. S2CID 14900395. Kim E, Lee Y, Choi S, Song JJ (July 2014 ... Cell. 122 (4): 633-44. doi:10.1016/j.cell.2005.06.012. PMID 16122429. S2CID 16706329. Mizutani A, Wang L, Rajan H, Vig PJ, ... This led to the hypothesis that the aggregates are toxic to neurons, but it has been shown in mice that aggregation is not ...
In addition, PGE2 inhibits platelet aggregation. PGE2 also suppresses T cell receptor signaling and proliferation, and may play ... PGE2 also has roles in inhibition of cytotoxic T-cell function, cell division of T-lymphocytes, and the development of TH1 ... "A live imaging cell motility screen identifies prostaglandin E2 as a T cell stop signal antagonist". Journal of Immunology. 187 ... Torres R, Picado C, de Mora F (January 2015). "The PGE2-EP2-mast cell axis: an antiasthma mechanism". Molecular Immunology. 63 ...
Cell lysis is a convenient tool to isolate certain macromolecules that exist primarily inside of the cell. Cell membranes ... At 303 K (30 °C) it forms micelles with aggregation number 75-120 (depending on method of determination; average ~95) and ... Other toxicity tests have been conducted using incubated human skin HaCaT keratinocyte cells. These human cells were incubated ... The human cells show CTAB being nontoxic at concentrations less than 1 μM. Without the use of CTAB in this synthesis, the gold ...
... and colocalization in cultured cells". In Vitro Cell. Dev. Biol. Anim. 38 (10): 582-94. doi:10.1290/1543-706X(2002)38. 2.0.CO;2 ... Moss TN, Vo A, McKeehan WL, Liu L (2007). "UXT (Ubiquitously Expressed Transcript) causes mitochondrial aggregation". In Vitro ... and regulated expression in prostate cancer cells results in decreased cell proliferation. Significantly, because decreased ... Cell. 13 (2): 670-82. doi:10.1091/mbc.01-10-0513. PMC 65658. PMID 11854421. Nwachukwu JC, Li W, Pineda-Torra I, Huang HY, Ruoff ...
Dobson CM (February 2004). "Principles of protein folding, misfolding and aggregation". Seminars in Cell & Developmental ... Auxiliary factors inside the living cell such as folding catalysts and chaperones assist in the folding process but do not ... that a protein's native state corresponds to its free energy minimum under the solution conditions usually encountered in cells ...
... normally aids in protein folding and aggregation; when present in the cell, functioning as an anti-inflammatory molecule ... Similarly, Hsp70 overexpression suppressed poly-Q dependent aggregation and neurodegeneration in cell cultures, yeast, fly, and ... Given the role of heat shock proteins as an ancient defense system for stabilizing cells and eliminating old and damaged cells ... Hsp70 is overexpressed in malignant melanoma and underexpressed in renal cell cancer. In breast cancer cell line (MCF7) has ...
The classic taxonomic criterion has been the cell morphology and the plane of cell division. In Pleurocapsales, the cells have ... Aggregation must divert resources away from the core business of making more cyanobacteria, as it generally involves the ... The cells in a hormogonium are often thinner than in the vegetative state, and the cells on either end of the motile chain may ... Each individual cell (each single cyanobacterium) typically has a thick, gelatinous cell wall. They lack flagella, but ...
Wang, Michelle Dong (1993). Reaction and aggregation dynamics of cell surface receptors. OCLC 713128957. "Michelle D. Wang". ... including RNA polymerase molecules which move along the DNA template during cell division. During this process genetic ...
These byproducts can react with cellular proteins to cause misfolding and aggregation (especially in nondividing cells like ... The unregulated cell division that marks cancer development requires increased protein synthesis for cancer cell function and ... This can change the sensitivity of cancer cells to antineoplastic drugs; cancer cells either die at a lower drug concentration ... This increased protein synthesis is typically seen in proteins that modulate cell metabolism and growth processes. Cancer cells ...
found that CB causes multinucleation in cells and significantly affects cell motility. The multinucleated cells probably arise ... Cytochalasin B inhibits glucose transport and platelet aggregation. It blocks adenosine-induced apoptotic body formation ... Cells treated with cytochalasin B and control group cells could not be distinguished. This indicated, that CB has no ... These are small nuclei containing one chromosome or part of a chromosome which did not get to one of the cell poles during cell ...
Ice crystals can damage cell membranes directly. In addition, ice crystals can damage small blood vessels at the site of injury ... Platelet aggregation is another possible mechanism of injury. Blisters and spasm of blood vessels (vasospasm) can develop after ... inflammatory mast cells act in the area. Small clots (microthrombi) form and can cut off blood to the affected area (known as ...
Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene. GRCh38: Ensembl ... The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a ... Cell. Proteomics. 3 (11): 1093-101. doi:10.1074/mcp.M400085-MCP200. PMID 15345747. S2CID 18659460. Rush J, Moritz A, Lee KA, et ... 2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94-101. doi:10.1038/ ...
Tartaglia GG, Pawar AP, Campioni S, Dobson CM, Chiti F, Vendruscolo M (4 July 2008). "Prediction of aggregation-prone regions ... Nature Reviews Molecular Cell Biology. 15 (6): 384-96. doi:10.1038/nrm3810. PMID 24854788. S2CID 46357173. "Membership to EMBO ... Vendruscolo provided contributions in the field of protein folding, misfolding and aggregation. He introduced the approach of ... He edited computational programs able to predict essential characteristics of protein folding and protein aggregation. In a ...
These kinds of chimeras can be made through either aggregation of stem cells and the diploid embryo or injection of the stem ... ES cells from the inner cell mass of an implanted blastocyst can contribute to all cell lineages of a mouse including the germ ... ES cells can be used in combination with eight-cell-and two-cell-stage embryos to make chimeras and exclusively give rise to ... Often, most or all of the cells of a single cell type will be composed of a single cell line, i.e. the blood may be composed ...
There are about 24,000 oxidative DNA adducts per cell in young rats and 66,000 adducts per cell in old rats. Likewise, any ... Excessive oxidative-damage leads to protein degradation or aggregation. The reaction of transition metals with proteins ... Reactive oxygen species can disrupt the function of immune cells, promoting immune evasion of leukemic cells. On the other hand ... In rat urine, about 74,000 oxidative DNA adducts per cell are excreted daily. There is also a steady state level of oxidative ...
"Widespread Proteome Remodeling and Aggregation in Aging C. elegans". Cell. 161 (4): 919-32. doi:10.1016/j.cell.2015.03.032. PMC ... Cell Stem Cell. 29: 918-932, doi: 10.1016/j.stem.2022.04.018 (2022). Morimoto, R.I., and G.S. Budinger. Protein Folding ... Krammer, C.; Park, K.W.; Li, L.; Melki, R.; Morimoto, R.I. (2013). "Spreading of a Prion Domain from Cell to Cell by Vesicular ... Rethinking the Roles of HSF-1 in Cell Stress, Development and Organismal Health. Trends in Cell Biology 12: DOI: org/10.1016/ j ...
In eukaryotic cells, an aggresome refers to an aggregation of misfolded proteins in the cell, formed when the protein ... Consequently, elaborate systems have evolved to protect cells from the deleterious effects of misfolded proteins. Cells mainly ... An aggresome forms around the microtubule organizing center in eukaryotic cells, adjacent to or enveloping the cell's ... Overexpression or inhibition of proteasome activity in transfected human embryonic kidney cells or Chinese hamster ovary cells ...
Identification of a cell surface protein involved in calcium-dependent cell aggregation". Cell. 28 (2): 217-224. doi:10.1016/ ... They found the hemagglutinin directly interacted with the e-cadherin in the epithelial cells to disturbed cell to cell adhesion ... Takeichi, M (1977). "Functional correlation between cell adhesive properties and some cell surface proteins". J. Cell Biol. 75 ... Once treated, these cells were tested for aggregation levels. He found that both calcium dependent and calcium independent ...
... aggregation-prone mutants of the tumor-suppressor protein p53; and semen proteins whose aggregation enhances HIV infection. ... which kills pancreatic β-cells in type-2 diabetes; transthyretin (TTR), which causes familial amyloid polyneuropathy, familial ... Lysine-specific molecular tweezers are broad-spectrum inhibitor of aggregation and toxicity of amyloid proteins. J. Am. Chem. ... The Lys-specific molecular tweezer, CLR01, modulates aggregation of mutant p53 DNA binding domain and inhibits its toxicity, ...
"Arfaptin 2 regulates the aggregation of mutant huntingtin protein". Nature Cell Biology. 4 (3): 240-5. doi:10.1038/ncb761. PMID ... Molecular Biology of the Cell. 14 (9): 3767-81. doi:10.1091/mbc.E03-01-0864. PMC 196566. PMID 12972563. Rangone H, Pardo R, ...
"Transient aggregation of ubiquitinated proteins during dendritic cell maturation". Nature. 417 (6885): 177-82. doi:10.1038/ ... Subsequently, research at the CIML expanded to other cells of the immune system, including B cells, dendritic cells and natural ... 2012). "Tuning of Natural Killer Cell Reactivity by NKp46 and Helios Calibrates T Cell Responses". Science. 335 (6066): 344-8. ... 2009). "MafB restricts M-CSF-dependent myeloid commitment divisions of hematopoietic stem cells". Cell. 138 (2): 300-13. doi: ...
Both modifications enhance BiP's ability to prevent protein aggregation. In mice cells, the conserved cysteine pair forms a ... These cysteines have been shown to undergo post-translational modifications in both yeast and mammalian cells. In yeast cells, ... In contrast, viruses rely on host BiP to successfully replicate, largely by infecting cells through cell-surface BiP, ... "Inhibition of antigen-presenting cell function and stimulation of human peripheral blood mononuclear cells to express an ...
Tuchin DA, Erve PR, Schumer W (1976). "Prevention of endotoxin--induced irreversible platelet aggregation in vitro". Surgical ... Molecular Cell. 19 (1): 89-100. doi:10.1016/j.molcel.2005.05.015. PMID 15989967. Wada O, Oishi H, Takada I, Yanagisawa J, Yano ... Molecular Cell. 3 (1): 97-108. doi:10.1016/S1097-2765(00)80178-1. PMID 10024883. Donner AJ, Ebmeier CC, Taatjes DJ, Espinosa JM ...
... the transient auto-aggregation of these cells in suspension and equally transient fall in circulating neutrophils when injected ... Thus, fMLF can act as a find-me signal, released by dead or dying cells to attract phagocytes to those cells, so that the ... Follow-up studies found that the genes of mitochondria and chloroplasts organelles of Eukaryote cells, including those of ... O'Flaherty JT, Kreutzer DL, Showell HS, Becker EL, Ward PA (Dec 1978). "Desensitization of the neutrophil aggregation response ...
Initial cell spreading on the abductin-based protein was similar to that on bovine serum albumin. These studies thus ... A moderate increase in DH was observed from 35 °C, and a sharper increase in DH occurred starting at 57 °C (aggregation ... A LIVE/DEAD assay revealed that human umbilical vein endothelial cells had a viability of 98 ± 4% after being cultured for two ... The abductin-based protein was cytocompatible, and cells spread slowly when first seeded on the abductin-based protein. ...
Cell Biol. Advances in Molecular and Cell Biology. Vol. 14. pp. 29-55. doi:10.1016/S1569-2558(08)60339-2. ISBN 9780762301133. . ... while aberrant PPIs are the basis of multiple aggregation-related diseases, such as Creutzfeldt-Jakob and Alzheimer's diseases ... The activity of the cell is regulated by extracellular signals. Signal propagation inside and/or along the interior of cells ... Cooper G (2000). The cell : a molecular approach (2nd ed.). Washington DC: ASM Press. ISBN 978-0-87893-106-4. .. [page needed] ...
Studies focusing on human breast cancer cell lines have further demonstrated that these cancerous cells display an increased ... "State of aggregation". Nature Neuroscience. 14 (4): 399. April 2011. doi:10.1038/nn0411-399. PMID 21445061.. ... Anti-aggregation agents[84] such as apomorphine, or carbenoxolone. The latter has commonly been used as a treatment for peptic ... Molecular Cell Biology. 8 (2): 101-12. doi:10.1038/nrm2101. PMID 17245412. S2CID 32991755.. ...
To disrupt the Greco, they plan to use a magnetron disguised as a new cell phone as a gift to Bank. They also obtain the ... On review aggregation website Rotten Tomatoes, the film holds an approval rating of 70% based on 200 reviews, and an average ...
"Brawl in Cell Block 99". Rotten Tomatoes. Retrieved October 16, 2017.. *^ "The 18 most unforgettable films of 2017, from 'Brawl ... The review aggregation website Rotten Tomatoes, the film has an "certified fresh" approval rating of 92% based on 75 reviews, ... "Brawl in Cell Block 99. 2017. Directed by S. Craig Zahler - MoMA". The Museum of Modern Art.. ... Zahler's second feature film as writer, director, and co-composer was Brawl in Cell Block 99, which stars Vince Vaughn, ...
A cell-counting assay may determine the number of living cells, the number of dead cells, or the ratio of one cell type to ... A similar assay but used for a different purpose is called Ristocetin Induced Platelet Aggregation or RIPA, which tests ... Other cell assaysEdit. Many cell assays have been developed to assess specific parameters or response of cells (biomarkers, ... CellTiter-Glo® Luminescent Cell Viability Assay. *Cell counting instruments and methods: CASY cell counting technology, Coulter ...
Cell. 2006-06-16, 125 (6): 1179-1191 [2018-08-27]. ISSN 0092-8674. PMID 16777606. doi:10.1016/j.cell.2006.04.026. (原始内容存档于2018- ... Tau aggregation inhibitor (TAI) therapy with remberTM arrests disease progression in mild and moderate Alzheimer's disease over ... Cell. 2012, 148 (6): 1204-22. PMC 3319071 . PMID 22424230. doi:10.1016/j.cell.2012.02.040.. ... While scientists know Alzheimer's disease involves progressive brain cell failure, the reason cells fail isn't clear.. ...
... is an aggregation of cells that eventually forms a tooth and is organized into three parts: the enamel organ, the dental ... The dental papilla contains cells that develop into odontoblasts, which are dentin-forming cells.[3] Additionally, the junction ... These cells give rise to ameloblasts, which produce enamel and the reduced enamel epithelium. The growth of cervical loop cells ... Tooth development is the complex process by which teeth form from embryonic cells, grow, and erupt into the mouth. Although ...
The cells are arranged in horizontal layers named combs, each cell being vertical and closed at the top. An egg is then laid in ... a component of the honey bee worker aggregation pheromone, that could therefore represent an honest signal for hornets. ... To be able to build cells in total darkness, they apparently use gravity to aid them.[clarification needed][citation needed] At ... As the colony size grows, new combs are added, and an envelope is built around the cell layers until the nest is entirely ...
... status has also been implicated in the maintenance of normal endothelial cell function of cells lining the inner ... surface of arteries, anti-inflammatory activity and inhibition of platelet adhesion and aggregation.[85] An inverse relation ... These convey α-tocopherol to cells in the rest of the body. As an example of a result of the preferential treatment, the US ... Alpha-tocopherol is a lipid-soluble antioxidant functioning within the glutathione peroxidase pathway,[15] and protecting cell ...
Aggregation[edit]. Aggregation in colloidal dispersions (or stable suspensions) has been characterized by the degree of ... This type of ordered array has also been observed in cell suspensions, where the symmetry is well adapted to the mode of ... Modes of Aggregation of the Virus Particles". The Journal of General Physiology. 25 (1): 111-46. doi:10.1085/jgp.25.1.111. PMC ... Witten, T.; Sander, L. (1983). "Diffusion-limited aggregation". Physical Review B. 27 (9): 5686. Bibcode:1983PhRvB..27.5686W. ...
Just as in animals, plant cells differentiate and develop into multiple cell types. Totipotent meristematic cells can ... protein expression is induced by stresses and protects other proteins from aggregation as a result of desiccation and freezing. ... Cell division is also characterized by the development of a phragmoplast for the construction of a cell plate in the late ... Plant cells are typically distinguished by their large water-filled central vacuole, chloroplasts, and rigid cell walls that ...
Thus, protective cells termed astrocytes surround the capillaries in the brain and absorb nutrients from the blood and ... "A Model for β-Amyloid Aggregation and Neurotoxicity Based on Free Radical Generation by the Peptide: Relevance to Alzheimer ... Neurotoxins inhibit neuron control over ion concentrations across the cell membrane,[6] or communication between neurons across ... Toxic effects from anatoxin-a progress very rapidly because it acts directly on the nerve cells (neurons). The progressive ...
It is produced in the gastric chief cells of the stomach lining and is one of the main digestive enzymes in the digestive ... cellular aggregation via surface antigens, or rosetting assays.[45] ... Pepsin is retained within the cell for up to 24 hours.[27] Such exposure to pepsin at neutral pH and endocyctosis of pepsin ... Pepsin's proenzyme, pepsinogen, is released by the gastric chief cells in the stomach wall, and upon mixing with the ...
"J. Cell Biol. 195 (4): 617-29. doi:10.1083/jcb.201106037. PMC 3257523. PMID 22065637.. ... Ellis RJ, Minton AP (May 2006). "Protein aggregation in crowded environments". Biological Chemistry. 387 (5): 485-97. doi: ... "Molecular Cell. 23 (5): 697-707. doi:10.1016/j.molcel.2006.07.016. PMC 5704897. PMID 16949366.. ... Dominance interactions in Escherichia coli cells mixedly infected with bacteriophage T4D wild-type and amber mutants and their ...
... are formed by aggregations of the medullary epithelial cells.[3] These are concentric, layered whorls of epithelial cells that ... Each T cell has a distinct T cell receptor, suited to a specific substance, called an antigen.[12] Most T cell receptors bind ... T cell maturationEdit. The thymus facilitates the maturation of T cells, an important part of the immune system providing cell- ... Here, the T cell receptor interacts with the MHC molecules on the surface of epithelial cells.[13] A T cell with a receptor ...
Cell phones communicating with a single cell tower constitute a local subnetwork; the connection between the cell tower and the ... Connecting DSLAMs to the nearest ATM or Ethernet aggregation node.. *Connecting a large company's site to a metro Ethernet ... A backhaul of a mobile network, also referred to as mobile-backhaul connects a cell site towards the core network. The two main ...
While scientists know Alzheimer's disease involves progressive brain cell failure, the reason cells fail isn't clear.. ... Wischik Claude M, Bentham Peter, Wischik Damon J, Seng Kwang Meng (2008). «Tau aggregation inhibitor (TAI) therapy with ... Int J Biochem Cell Biol. 37 (2): 289-305. *↑ «Cópia arquivada». Consultado em 24 de julho de 2015. Arquivado do original em 26 ... doi:10.1016/j.cell.2012.02.040. *↑ Yankner BA, Duffy LK, Kirschner DA (1990). «Neurotrophic and Neurotoxic Effects of Amyloid ...
Hydrogen fuel cell vehicles (FCV) with tanks containing 5.6 kg of hydrogen can deliver more than 90 kWh of electricity.[6] ... "SwRI deploys novel vehicle-to-grid aggregation system". Southwest Research Institute. 2013-09-09. Retrieved 2015-02-26.. ... "SwRI develops first ERCOT-qualified vehicle-to-grid aggregation system". Southwest Research Institute. 2014-01-14. Retrieved ... Most modern battery electric vehicles use lithium-ion cells that offer round-trip efficiency greater than 90%.[25] Efficiency ...
The use of quarter-row segments is driven by the fact that DRAM cells are narrower than SRAM cells. The SRAM bits are designed ... Row access is the heart of a read operation, as it involves the careful sensing of the tiny signals in DRAM memory cells; it is ... This operation has the side effect of refreshing the dynamic (capacitive) memory storage cells of that row. ...
Pugliese A, Beltramo T, Torre D (October 2008). "Reye's and Reye's-like syndromes". Cell Biochemistry and Function. 26 (7): 741 ... Thromboxanes are responsible for the aggregation of platelets that form blood clots. Heart attacks are caused primarily by ... Acetylsalicylic acid is quickly absorbed through the cell membrane in the acidic conditions of the stomach. The increased pH ... Endothelial cells lining the microvasculature in the body are proposed to express PTGS2, and, by selectively inhibiting PTGS2, ...
2003). "Porphyromonas gingivalis induces murine macrophage foam cell formation". Microb Pathog 35 (6): 259-67. doi:10.1016/j. ... "Activation of protease-activated receptors by gingipains from Porphyromonas gingivalis leads to platelet aggregation: a new ... "Intracoronary bone marrow-derived progenitor cells in acute myocardial infarction". N Engl J Med 2006 355 (12): 1210-21. doi: ...
To cite and quote an archived, two-author, foreign language journal article re-published as a PDF on an information aggregation ... on lipid peroxidation of liver mitochondria and red blood cell in rats]". Wei Sheng Yan Jiu (in Chinese). 28 (2): 115-116. PMID ... To cite and quote an archived, two-author, foreign language journal article re-published as a PDF on an information aggregation ... on lipid peroxidation of liver mitochondria and red blood cell in rats] ,journal=Wei Sheng Yan Jiu ,language=Chinese ,volume=28 ...
Notes and Domino is a cross-platform, distributed document-oriented NoSQL database and messaging framework and rapid application development environment that includes pre-built applications like email, calendar, etc. This sets it apart from its major commercial competitors, such as Microsoft Exchange or Novell GroupWise, which are purpose-built applications for mail and calendaring that offer APIs for extensibility. Domino databases are built using the Domino Designer client, available only for Microsoft Windows; standard user clients are available for Windows, Linux, and macOS.[17] A key feature of Notes is that many replicas of the same database can exist at the same time on different servers and clients, across dissimilar platforms; the same storage architecture is used for both client and server replicas. Originally, replication in Notes happened at document (i.e., record) level. With release of Notes 4 in 1996, replication was changed so that it now occurs at field level. A database is a ...
Among other things, arachidonic acid helps to maintain hippocampal cell membrane fluidity.[25] It also helps protect the brain ... and platelet aggregation[33] appear to be unaffected with this level and duration of use. Furthermore, higher concentrations of ... Darios, F; Davletov, B (2006). "Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3". ... of membranes of the body's cells, and is abundant in the brain, muscles, and liver. Skeletal muscle is an especially active ...
Cell. 122 (6): 957-68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923. ... Traut TW, Payne RC (Dec 1980). "Dependence of the catalytic activities on the aggregation and conformation states of uridine 5 ... Traut TW, Payne RC, Jones ME (Dec 1980). "Dependence of the aggregation and conformation states of uridine 5'-phosphate ... Lin T, Suttle DP (May 1995). "UMP synthase activity expressed in deficient hamster cells by separate transferase and ...
Mie theory has been used to determine whether scattered light from tissue corresponds to healthy or cancerous cell nuclei using ... I. Theory for external aggregation", J. Opt. Soc. Am. A 11, 3251-3260 (1994) K. Frizyuk, I. Volkovskaya, D. Smirnova, A. ... and biological cells and cellular components, a more detailed approach is necessary. The Mie solution is named after its ...
The thallus forms areas with cracked patches of goniocysts (small aggregations of photobiont cells enclosed by hyphae forming a ... The photobiont partner of the lichen is chlorococcoid, and they have cells that are typically 5-12 μm in diameter. ...
... and as we now see that all the forms of reproduction depend on the aggregation of gemmules derived from the whole body, we can ... rejected the idea that these particles were thrown off from all the cells of the body. He called the particles "pangens", later ...
Within the fruiting bodies, cells begin as rod-shaped vegetative cells, and develop into rounded myxospores with thick cell ... Individuals benefit from aggregation as it allows accumulation of the extracellular enzymes that are used to digest food; this ... The fruiting process is thought to benefit myxobacteria by ensuring that cell growth is resumed with a group (swarm) of ... When nutrients are scarce, myxobacterial cells aggregate into fruiting bodies (not to be confused with those in fungi), a ...
... is also called Dual Cell in the context of UMTS/HSPA+. Through carrier aggregation (part of the UMTS ... Release 10 supports four-carrier aggregation and eight-carrier-aggregation is supported since Release 11. 3GPP standardized ... Carrier aggregation is a technique that is used in wireless communication to increase the data rate per user, whereby multiple ... The sum data rate of a cell is increased as well because of a better resource utilization. In addition load balancing: p.42 is ...
Antibodies for proteins involved in cell aggregation pathways, according to their Panther/Gene Ontology Classification ...
Cell declines appear driven by hetero-aggregation and co-sedimentation of cells with plastic particles, recorded visually and ... Cell size matters : nano- and micro-plastics preferentially drive declines of large marine phytoplankton due to co-aggregation ... WRAP-Cell-size-matters-nano-micro-plastics-drive-marine-phytoplankton-co-aggregation-2022.pdf - Published Version - Requires a ... 2022) Cell size matters : nano- and micro-plastics preferentially drive declines of large marine phytoplankton due to co- ...
In this study, these two compounds were tested for their effects on hCT aggregation. We found that Mag and Hon both inhibited ... Further immuno-dot blot and dynamic light scattering studies suggested Mag and Hon suppressed the aggregation of hCT both at ... Human calcitonin (hCT) is a typical amyloidogenic peptide, its aggregation is associated with medullary carcinoma of the ... MTT cell toxicity assay. MTT-based cell toxicity assays were performed as we previously described79. SH-SY5Y cells were ...
Trodusquemine enhances Aβ42 aggregation but suppresses its toxicity by displacing oligomers from cell membranes. I: Nature ... Trodusquemine enhances Aβ42 aggregation but suppresses its toxicity by displacing oligomers from cell membranes. Nature ... Trodusquemine enhances Aβ42 aggregation but suppresses its toxicity by displacing oligomers from cell membranes. / Limbocker, ... Trodusquemine enhances Aβ42 aggregation but suppresses its toxicity by displacing oligomers from cell membranes. ...
Shift in aggregation, ROS generation, antioxidative defense, lysozyme and acetylcholinesterase activities in the cells of an ... Home › Related Articles › Shift in aggregation, ROS generation, antioxidative defense, lysozyme and acetylcholinesterase ... Prolonged treatment of washing soda inhibited the degree of cellular aggregation. Experimental exposure of 8 and 16mg/l of ... Present investigation deals with estimation of cellular aggregation, generation of ROS and activities of antioxidant enzymes, ...
A multi-bucket aggregation that works on geo_point. fields and groups points into buckets that represent cells in a grid. The ... To allow for more accurate counting of the top cells returned in the final result the aggregation defaults to returning max(10 ... The table below shows the metric dimensions for cells covered by various string lengths of geohash. Cell dimensions vary with ... aggregation as bucket keys can be also used for "zooming in" by translating them into bounding boxes using one of available ...
Importantly, we discovered that aSYN protein aggregation is modulated by patient brain cells intracellular milieus at the ... We found increased β-sheets and aggregated aSYN in PD patient hiPSC-derived midbrain cells, compared to controls. ... Using human induced pluripotent stem cells (hiPSC) and biochemical and vibrational spectroscopy techniques, we studied whether ... Recent studies suggest that brain cell type specific intracellular environments may play important roles in the generation of ...
We then constructed GJB1 expression vectors and performed cell biological analysis in vitro. Expression of FLAG-tagged GJB1 at ... Moreover, intracellular aggregation probably occurs in the endoplasmic reticulum compartment rather than the Golgi apparatus. ... Moreover, intracellular aggregation probably occurs in the endoplasmic reticulum compartment rather than the Golgi apparatus. ... Expression of FLAG-tagged GJB1 at various time points after transfection revealed evident protein aggregation with both wild- ...
Identification of an ERK Inhibitor as a Therapeutic Drug Against Tau Aggregation in a New Cell-Based Assay ... Identification of an ERK Inhibitor as a Therapeutic Drug Against Tau Aggregation in a New Cell-Based Assay ...
Figure 3: Resveratrol prevents p53 aggregation in breast cancer cells. (A) MDA-MB-231, (C) HCC-70 and (E) MCF-7 cells were ... Cell proliferation was assessed using the trypan blue exclusion test of cell viability. MDA-MB-231 cells cultured in a 24-well ... Resveratrol inhibits mutant p53 aggregation in MDA-MB-231 cells and also reduced cell migration and proliferation. These ... resveratrol reduces mutant p53 protein aggregation in MDA-MB-231 and HCC-70 cells but not in the wild-type p53 cell line MCF-7 ...
However, as cells age, these control mechanisms are weakened and the cell is less able to resolve the aggregates. The ... In mammalian cells, these protein aggregates are termed "aggresomes" and they are formed when the cell is diseased. This is ... After the cell divides, the daughter cells with the older pole gets the protein aggregate and grows more slowly than daughter ... mammalian cells).[citation needed] The aggregates in bacteria asymmetrically end up at one of the poles of the cell, the "older ...
Adding Dextran T-70 to the cell suspension increased erythrocyte aggregation in the tube, which resulted in suppressed cell ... The rate of O(2) release from the cells flowing in the narrow tube was determined based on the visible absorption spectrum and ... flowing in narrow tubes is inhibited primarily by erythrocyte aggregation itself and partly by thickening of the cell-free ... The deoxygenation was inversely proportional to the cell-free-layer thickness. The relation was not essentially altered even ...
Aggregation of alpha-synuclein (SNCA) may underlie the pathology of PD. They are the main components of Lewy bodies and ... Protein aggregation is a prominent feature of many neurodegenerative disorders including Parkinsons disease (PD). ... Protein aggregation is a prominent feature of many neurodegenerative disorders including Parkinsons disease (PD). Aggregation ... The aggregation was blocked by 100 nM EGCG in a concentration-dependent manner, as shown by an absence of thioflavin T binding ...
Study reports that PEAR1 methylation is a marker of platelet activation and whole blood cells count variability, in turn ... platelet endothelial aggregation receptor 1provided by HGNC. Primary source. HGNC:HGNC:33631 See related. Ensembl: ... platelet endothelial aggregation receptor 1. Names. multiple EGF-like domains protein 12. multiple EGF-like-domains 12. ... XM_017001236.2 → XP_016856725.1 platelet endothelial aggregation receptor 1 isoform X3. UniProtKB/Swiss-Prot. Q5VY43, Q8TEK2. ...
A one-dimensional model for cell diffusion and aggregation, incorporating volume filling and cell-to-cell adhesion, J. Math. ... R. M. Mège , J. Gavard and M. Lambert , Regulation of cell-cell junctions by the cytoskeleton, Current Opinion in Cell Biology ... S. Mancini , R. M. Mège , B. Sarels and P. O. Strale , A phenomenological model of cell-cell adhesion mediated by cadherins, J ... Cell Mechanics: From Single Scale-Based Models to Multi-Scale Modeling, Editors: A. Chauviere, L. Preziosi and C. Verdier, ...
Multicolumn variables and cell arrays other than cell arrays of character vectors are not allowed. ... Ensemble aggregation method. AdaBoostM1. , LogitBoost. , GentleBoost. , RUSBoost. , Subspace. , Bag. , AdaBoostM2 ... If the ensemble-aggregation method (. Method. ) is bag. and:. *. The misclassification cost (. Cost. ) is highly imbalanced, ... String array or cell array of character vectors. Each element in the array is the name of a predictor variable. The names must ...
Hero proteins can also block several different types of pathological protein aggregations in cells, and in Drosophila strains ... A widespread family of heat-resistant obscure (Hero) proteins protect against protein instability and aggregation. View ORCID ... A widespread family of heat-resistant obscure (Hero) proteins protect against protein instability and aggregation ... A widespread family of heat-resistant obscure (Hero) proteins protect against protein instability and aggregation ...
Secondary nucleation of monomers on fibril surface dominates α-synuclein aggregation and provides autocatalytic amyloid ... Lee, S., Desplats, P., Lee, H., Spencer, B. & Masliah, E. (2012). Cell-to-cell transmission of α-synuclein aggregates. Amyloid ... 3. Seeding efficiency in α-syn aggregation. (a-c) Representative seeded aggregation kinetic traces in the presence of fixed (3 ... 3.1. Non-seeded aggregation kinetic experiments. α-Syn aggregation is strongly influenced by intrinsic and extrinsic factors. ...
Somatic mutations inevitably occur during the many cell divisions required for the single-cell zygote to develop into a full ... Familial aggregation of amyotrophic lateral sclerosis, dementia, and Parkinsons disease: evidence of shared genetic ... with cell-to-cell transmission of the misfolded protein within the motor network. The difference from classic prion disease is ... Thus, formation of pathologic TDP-43 and its ubiquination may prove to be a mechanism of cell death that is not specific to ALS ...
Thermo Fisher cutting jobs at NY cell culture media plant. *. Sponsored Content. Grow Your CDMO With the Addition of Gloveless ... Bioprocessing begins upstream, most often with culturing of animal or microbial cells in a range of vessel types (such as bags ...
Parmeggiani F, Costagliola C, DAngelo S, Incorvaia C, Perri P, Sebastiani A. Clear cell renal cell carcinoma associated with ... These accumulations vary from dome-shaped deposits over the RPE, to linear and vertical aggregations, and other irregular ... Extensive mononuclear cell infiltrate in the choriocapillaris and choroid, as reported in histopathology, can be observed as ... A mild to moderate anterior chamber and vitreous cell reaction may be present even in the inactive stage. The patient often has ...
The algae cells were then rinsed with Milli Q water to remove Hg2+ attached to the outside of cells. After rinsing once, the ... Euglena gracilis cells were harvested from the culture medium by centrifugation, and were adjusted to a cell density of 1.0 × ... 3 (a) Recovery ratio (mobile cells/total cells by number) of algae incubated with different concentrations of Hg2+ at 10, 20, ... The Euglena gracilis cell lacks a cell wall. Instead, it has a pellicle made up of a protein layer supported by a substructure ...
LTE pioneer SK Telecom said it has successfully developed LTE-Advanced tri-band carrier aggregation technology. The technology ... It will boost capacity per cell site. In areas such as a crossroads in Seoul where many people might be streaming video, it ... LTE pioneer SK Telecom has successfully developed LTE-Advanced tri-band carrier aggregation technology, to ease network ...
Eberhard W. Lisse \ / Obstetrician & Gynaecologist (Saar) , el at lisse.NA el108-ARIN / * , Telephone: +264 81 124 6733 (cell) ... R] Odp: aggregation question. Petr PIKAL petr.pikal at precheza.cz Thu Jul 7 12:08:25 CEST 2011 *Previous message: [R] ...
Special Aggregation Levels. This table shows a small number of special aggregation levels that have only a few data cells ... e) Aggregation by size of establishment is done for private ownership cells only. ... QCEW Aggregation Level Codes (a) for NAICS Coded Data. Level Code. Aggregation Level. Notes. ... Aggregation Level Codes Matrix. This table shows the main aggregation level codes as a matrix. Tabular presentation may be ...
Am J Respir Cell Mol Biol 2011; 45: 858-866.. OpenUrlCrossRefPubMed ... Summary of previous studies on familial aggregation and heritability of sarcoidosis. Only familial aggregation studies with a ... Familial aggregation and heritability of sarcoidosis: a Swedish nested case−control study. Marios Rossides, Johan Grunewald, ... Familial aggregation of sarcoidosis. A case-control etiologic study of sarcoidosis (ACCESS). Am J Respir Crit Care Med 2001; ...
141 cases of early stage non-small cell lung cancer were stained for TP and vascular grade using the P-GF.44C and JC70 MoAbs, ... The early steps of TP activation could be identified in 27 cases, where one or two foci of cancer cell TP overexpression ... The present study provides further evidence of a direct association of TP and the process of angiogenesis in non-small cell ... Platelet-derived endothelial cell growth factor (PD-ECGF) also called thymidine phosphorylaze (TP) has been shown to have ...
Although the exact function of this protein is unknown, it appears to play an important role in nerve cells (neurons) in the ... Bates G. Huntingtin aggregation and toxicity in Huntingtons disease. Lancet. 2003 May 10;361(9369):1642-4. doi: 10.1016/S0140- ... Within cells, this protein may be involved in chemical signaling, transporting materials, attaching (binding) to proteins and ... To be or not to be toxic: aggregations in Huntington and Alzheimer disease. Trends Genet. 2006 Aug;22(8):408-11. doi: 10.1016/j ...
  • In molecular biology, protein aggregation is a phenomenon in which intrinsically-disordered or mis-folded proteins aggregate (i.e., accumulate and clump together) either intra- or extracellularly. (wikipedia.org)
  • In these cases, if the cell does not assist the protein in re-folding, or degrade the unfolded protein, the unfolded/misfolded protein may aggregate, in which the exposed hydrophobic portions of the protein may interact with the exposed hydrophobic patches of other proteins. (wikipedia.org)
  • citation needed] In addition to mutations in the affected proteins themselves, protein aggregation could also be caused indirectly through mutations in proteins in regulatory pathways such as the refolding pathway (molecular chaperones) or the ubiquitin-proteasome pathway (ubiquitin ligases). (wikipedia.org)
  • Hero proteins can also block several different types of pathological protein aggregations in cells, and in Drosophila strains that model neurodegenerative diseases. (biorxiv.org)
  • Within cells, this protein may be involved in chemical signaling, transporting materials, attaching (binding) to proteins and other structures, and protecting the cell from self-destruction (apoptosis). (medlineplus.gov)
  • Cell fusion is regulated by snap receptor (SNARE) proteins in mammalian cells. (purdue.edu)
  • The zippering of SNARE proteins exerts forces to the adjacent cell membrane and induces membrane fusion. (purdue.edu)
  • In a healthy cell, the production and degradation of protein are integrated with processes that ensure the proper folding of these proteins and the prevention of unfolded proteins aggregating. (edu.au)
  • The Ecroyd research focus is in the field of protein homeostasis (proteostasis), an important area of research as disturbances in proteostasis can lead to protein aggregation (i.e. the clumping of proteins into large deposits), a pathological hallmark of many human diseases, including Alzheimer's disease, Parkinson's disease and Motor Neurone Disease (MND). (edu.au)
  • The group are also developing new single-molecule approaches so that, for the first time, we can see and characterise the interactions between heat-shock proteins and aggregation-prone proteins. (edu.au)
  • Aggregates determine phenomena such as structural conformation in proteins and DNA, reduction of surface tension due to surfactants and the formation of biologically relevant structures such as micelles, important for fat storage within cells, or vesicles, playing an essential role for substance transport in living organisms. (sciepub.com)
  • The mechanism of protein aggregation is not well understood, "but most proteins will aggregate when they are in an unfolded or partially folded state," says Angelica Olcott, product manager at ProteinSimple. (biopharminternational.com)
  • this perturbs redox homeostasis, resulting in misfolding and aggregation of metastable proteins such as pulmonary surfactant protein SP-C. (E) SARS-CoV-2 in the blood, or in bronchoalveolar lavage fluid, might form an extra protein corona layer around itself - which might induce amyloid formation. (cell.com)
  • A dynamic instability triggered by the aggregation of the proteins may be at the origin of the symmetry breaking. (fresnel.fr)
  • PIG-A is required for synthesis of GPA, a cellular protein which allows a number of surface proteins to remain tethered to the cell wall. (managedcaremag.com)
  • On the one hand, you will have to run basic assays to quantify biomolecules such as nucleic acids and proteins, or cell viability . (bmglabtech.com)
  • These diseases, including Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis (Lou Gehrig's disease), and polyglutamine diseases, are associated with misfolding and aggregation of proteins in the central nervous system, which ultimately leads to neuronal cell death. (upenn.edu)
  • In this experiment, Luca Parmitano studies the aggregation and behaviour of amyloid proteins to analyse the possibility that astronauts are more likely to develop this type of disease. (euronews.com)
  • How do proteins fold in the cell, as they emerge out of the ribosome? (wisc.edu)
  • Proteins (blue chains and image in the front) are synthesized in the context of the ribosome (light green), in living cells. (wisc.edu)
  • We are involved in the study of fundamental questions in protein folding, including kinetic trapping events across the folding energy landscape, the role of water and hydrophobic collapse, the role of the protein's C terminus in folding, the thermodynamic balance between protein folding and aggregation in regular proteins and prions, and the prediction of protein structure from amino acid sequence. (wisc.edu)
  • Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains. (nih.gov)
  • In cells, proteins encoded by the same gene do not all behave uniformly but engage in functional subpopulations induced by spatial or temporal segregation. (uni-ulm.de)
  • Finally, a first SMT colocalization study of two proteins was performed in living cells showing enhanced SRF/MRTF-A colocalization upon stimulation. (uni-ulm.de)
  • They then conducted a series of tests and zeroed in on nine genes that made all proteins in the cell healthier. (medindia.net)
  • B ) Fluorescence microscopy of Lsm4-GFP cells co-expressing the mCherry-tagged PB proteins Dcp2 or Edc3. (elifesciences.org)
  • Let us natural viagra straight Generic proscar 5 mg for Border Clans which removed the proteins from the cells. (nephew.dk)
  • A clumping of proteins inside cell bodies in the brain, which may be toxic. (michaeljfox.org)
  • A chemical compound or substance that inhibits oxidation - damage to cells' membranes, proteins or genetic material by free radicals (the same chemical reaction that causes iron to rust). (michaeljfox.org)
  • Exposure of cells to stress, particularly oxidative stress, leads to misfolding of proteins and, if they are not refolded or degraded, to cytoplasmic protein aggregates. (uzh.ch)
  • This unfolded protein response (UPR) also promotes the degradation of misfolded proteins (ER-associated protein degradation-ERAD), but if these compensatory mechanisms fail, the affected cells may undergo apoptosis. (bmj.com)
  • Genetic silencing of WDFY4 in B cells increased lipidation of LC3 independent of p62 and Beclin1, which are essential proteins of canonical autophagy. (jimmunol.org)
  • To investigate factors that influence Tau protein aggregation, we expressed human Tau protein fused with fluorescent proteins in yeast Saccharomyces cerevisiae. (irb.hr)
  • Inhibiting hCT aggregation is thus of great importance both for pharmaceutical preparation of hCT in vitro and for the treatment of MTC. (nature.com)
  • We then constructed GJB1 expression vectors and performed cell biological analysis in vitro . (frontiersin.org)
  • We tested the potential of resveratrol, a naturally occurring polyphenol, to interact and prevent the aggregation of wild-type and mutant p53 in vitro using fluorescence spectroscopy techniques and in human breast cancer cells (MDA-MB-231, HCC-70 and MCF-7) using immunofluorescence co-localization assays. (oncotarget.com)
  • In addition, resveratrol and its derivatives pterostilbene and piceatannol inhibit mutant p53C aggregation in vitro . (oncotarget.com)
  • Shiga toxin-producing Escherichia coli (STEC) is a of STEC strains to invade epithelial cells in vitro, although pathogen that causes gastroenteritis and bloody diarrhea in small numbers ( 2,3 ), but no reports of invasion in vivo but can lead to severe disease, such as hemolytic uremic have been published. (cdc.gov)
  • We examined the aggregation-prone, EOFAD-related Arctic mutation (Abeta42Arc) and an artificial mutation (Abeta42art) that is known to suppress aggregation and toxicity of Abeta42 in vitro. (arctichealth.org)
  • Work currently being undertaken in this laboratory extends from molecular biology-based techniques to recombinant protein expression and purification, in vitro biochemical assays of chaperone protein activity, to mammalian cell culture and the study of protein expression and modification in animal tissues. (edu.au)
  • Polyanions have been reported to induce tau aggregation in vitro , but the precise trigger to convert tau from an inert to a seed-competent form in disease states is unknown. (jbc.org)
  • Thus, eukaryotic in vitro systems, specifically the wheat germ cell-free system, hold significant promise. (iucr.org)
  • Enlargement in vitro previous to mesenchymal stem cells (MSCs) software is a essential course of. (ncbcs.org)
  • Antibody Mediated Inhibition of TAU Aggregation in In vitro And in Neuronal cells. (mpg.de)
  • This complex of lectins, when applied in vitro to tunicate multipotent cells of epithelial origin, blocked cell proliferation and induced cell aggregation. (biologists.com)
  • Three-Dimensional In Vitro Cell Culture Models for Efficient Drug Discovery: Progress So Far and Future Prospects. (wjgnet.com)
  • The Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death. (wjgnet.com)
  • In vitro toxicity data of these metabolites derived in the development of validated and accepted in vitro and in silico embryonic stem cell test were used as input in the PBK model to extrapolate in vitro concentration-response curves to predicted approaches is urgently needed. (cdc.gov)
  • To verify the effects of resveratrol on tumorigenicity, cell proliferation and cell migration assays were performed using MDA-MB-231 cells. (oncotarget.com)
  • Significance: This study demonstrates how AQP4 aggregation influences plasma membrane dynamics to alter cell proliferation, invasiveness, migration, and apoptotic potential in glioma cells. (houstonmethodist.org)
  • A 2008 study found kaempferol-rich ginkgo biloba extract significantly inhibited the proliferation of pancreatic cancer cells. (mercola.com)
  • Mechanical properties of immune cells play important roles in multiple contexts: phagocytosis, immunological synapse formation, cell migration, cancer proliferation. (sfu.ca)
  • In those prostate cancer cells that depended on the predominant male sex hormone, testosterone, for their growth, capsaicin reduced cell proliferation in a dose-dependent manner. (naturalnews.com)
  • T cells may be responsible for regulating the proliferation, and thus the growth, of malignant epithelial cells in BCC. (who.int)
  • The relentless cell proliferation that characterizes malignancy is normally prevented by an intricate tumor suppression network, with its central hub being the preeminent tumor suppressor p53. (upenn.edu)
  • Our recent results have revealed a role for p53 in modulating metabolic pathways and autophagy that are crucial for cell proliferation. (upenn.edu)
  • that critically influences cell proliferation, differentiation and apoptosis ( Miele and Osborne, 1999 ). (elifesciences.org)
  • TXA 2 induces platelet activation, vasoconstriction, and vascular smooth muscle cell proliferation. (archbronconeumol.org)
  • Microglia are the resident immune cells of the brain and in neurodegeneration become pathologically activated leading to proliferation and release of cytotoxic cytokines. (biorxiv.org)
  • Cytotoxicity results show that cell viability and proliferation were not hindered in a wide range of nanocarrier concentrations and different incubation times. (dovepress.com)
  • The present study explored the effect of ginsenoside Rk1 (G-Rk1) on the proliferation and apoptosis in SK-OV-3 ovarian cancer cells and deepened its mechanism of action. (scialert.net)
  • It was observed that G-Rk1 can not only induce cell cycle arrest at the G1 phase but also inhibit the proliferation ability of SK-OV-3 cells. (scialert.net)
  • Therefore, the findings from the present study exhibited that G-Rk1 could inhibit the proliferation of SK-OV-3 ovarian cancer cells and maybe as a candidate drug for anti-ovarian cancer soon. (scialert.net)
  • Interestingly, ginsenoside Rg5, the isomer of G-Rk1, have been shown to inhibit the proliferation and promote apoptosis of ovarian cancer cells 12 . (scialert.net)
  • citation needed] Environmental stresses such as extreme temperatures and pH or oxidative stress can also lead to protein aggregation. (wikipedia.org)
  • This may lead to protein aggregation diseases, for example Parkinson's disease (PD), the prevalence of which is increasing (Dobson, Reference Dobson 2003 ). (cambridge.org)
  • Utilising a broad array of methods ranging from the fields of biophysics, biochemistry, and cell and molecular biology, we study the basic biological processes that lead to protein aggregation, with the aim of identifying and developing novel therapeutic strategies for the treatment of MND. (edu.au)
  • Yesica Nievas and colleagues report that disrupting palmitoylation reduced the protist's self-aggregation and adhesion to host cells. (asbmb.org)
  • Moreover, cell surface hydrophobicity is also important in adhesion because hydrophobic interaction tends to increase with increasing non-polar property of each surface. (kenyon.edu)
  • In addition, the authors observed platelet accumulation in combination with augmented expression of platelet endothelial cell adhesion molecule 1 (PECAM-1), as well as increased tissue factor and von Willebrand factor, in COVID-19 patients, suggesting that activation of the coagulation system in the brain vasculature most likely leads to occlusion and damage to small vessels. (medscape.com)
  • The article described how the scientists not only confirmed the two known host factors, the genes for the HIV co-receptors CD4 and CCR5, but also revealed the following host factors-tyrosylprotein sulfotransferase 2 (TPST2), solute carrier family 35 member B2 (SLC35B2), and activated leukocyte cell adhesion molecule (ALCAM. (genengnews.com)
  • The drug reduces abnormal platelet adhesion and aggregation. (nephew.dk)
  • A range of tests are needed to determine the efficacy of potential drugs, such as aggregation and adhesion/invasion assays. (tecan.com)
  • Aggregation and adhesion/invasion assays are among the many services SMALTIS off ers. (tecan.com)
  • Aggregation assays are used to assess the ability of a compound to inhibit adhesion, by interacting with the fi mbriae to cause aggregation. (tecan.com)
  • We also use the Spark to carry out adhesion/invasion assays to determine the capacity of bacterial strains to attach to or invade eukaryotic cells. (tecan.com)
  • We measure the blue and green fl uorescence, from the eukaryotic cells and bacteria respectively, to determine a ratio refl ecting the strain's adhesion/invasion capabilities. (tecan.com)
  • The Integrin Activation Pathway has been researched in relation to Cell Adhesion, Cell Migration, Platelet Aggregation, Localization, Secretion. (novusbio.com)
  • Nougayrède JP , Fernandes PJ , Donnenberg MS . Adhesion of enteropathogenic Escherichia coli to host cells. (cdc.gov)
  • Aggregation of alpha-synuclein (SNCA) may underlie the pathology of PD. (nih.gov)
  • Aggregation of the protein alpha-synuclein is found in Lewy bodies, a pathological hallmark of Parkinson's disease. (michaeljfox.org)
  • Washing soda, a reported environmental contaminant, affected adversely the immunophysiological status of E. carteri with reference to cellular aggregation, oxidative stress, antioxidative defense, lysozyme and acetylcholinesterase activity. (jostchemical.com)
  • The glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has multiple functions, including mediating oxidative stress-induced neuronal cell death. (cheric.org)
  • The GAI peptide also exerted a protective effect against oxidative stress-induced cell death in SH-SY5Y cells. (cheric.org)
  • This peptide could potentially serve as a tool to investigate GAPDH aggregation-related neurodegenerative and neuropsychiatric disorders and as a possible therapy for diseases associated with oxidative stress-induced cell death. (cheric.org)
  • Although etiology of neurodegenerative diseases is not well understood, some common pathological features are shared between them, including protein aggregation and oxidative stress. (bl.uk)
  • Abeta42 mutants with different aggregation profiles induce distinct pathologies in Drosophila. (arctichealth.org)
  • According to the authors, immunoglobulin complexes stimulate the classical complement cascade, activate endothelial cells, and subsequently induce blood-brain barrier leakage and immune cell infiltration. (medscape.com)
  • As revealed by transfection of tissue culture cells overexpressed p62 did not induce aggregation of regular CK filaments but selectively bound to misfolded and ubiquitinated CKs. (uzh.ch)
  • Cell Reports 33(9): 108418, December 2020. (upenn.edu)
  • Cell Rep, 2020 Aug 18. (nih.gov)
  • 2020 ) found that the removal of senescent cells from adult zebrafish with limb amputation injuries was harmful to tissue regeneration (Da Silva-Álvarez et al. (springer.com)
  • Self-aggregation plays an important role in chemical and biological processes, typically governed by simple chemical driving forces, e.g. hydrophilic and/or hydrophobic interactions, hydrogen bonds and pi-pi stacking 1 . (sciepub.com)
  • Further immuno-dot blot and dynamic light scattering studies suggested Mag and Hon suppressed the aggregation of hCT both at the oligomerization and the fibrillation stages, while MTT-based and dye-leakage assays demonstrated that Mag and Hon effectively reduced cytotoxicity caused by hCT aggregates. (nature.com)
  • Further evidence reveals significant intracellular stress granule formation induced by mutated GJB1 and impaired cell viability indicative of cytotoxicity of self-aggregates. (frontiersin.org)
  • Cells have mechanisms that can refold or degrade protein aggregates. (wikipedia.org)
  • However, as cells age, these control mechanisms are weakened and the cell is less able to resolve the aggregates. (wikipedia.org)
  • The present experiment may conclude that erythrocyte aggregation is induced preferentially among more deformable cells and/or among less negatively charged cells with weaker electrostatic repulsive force, and then the formed aggregates settle faster. (semanticscholar.org)
  • However, recent reports indicate that Abeta can form several different prefibrillar and fibrillar aggregates and that each aggregate may confer different pathogenic effects, suggesting that manipulation of Abeta42 aggregation may not only quantitatively but also qualitatively modify brain pathology. (arctichealth.org)
  • This pattern of degeneration coincides with the distribution of Thioflavin S-stained Abeta aggregates: Abeta42Arc formed large deposits in the cell body, Abeta42art accumulated preferentially in the neurites, while Abeta42 accumulated in both locations. (arctichealth.org)
  • Because the α-syn aggregates are deeply involved in the pathogenesis, there have been many attempts to demonstrate the mechanism of the aggregation and its potential causative factors including post-translational modifications (PTMs). (bmbreports.org)
  • α-Syn aggregates are detected in different cell types and intracellular locations, depending on the associated disease. (bmbreports.org)
  • Unexpectedly, these hyper-piliated cells have the ability to auto-aggregate via direct pilus-pilus interaction, and in liquid culture form macroscopic aggregates that rapidly sediment. (europa.eu)
  • The aggregates expressed a homolog of the integrin α-chain and other differentiation markers specific for epithelial cells. (biologists.com)
  • Aggregation is prevented by a crucial kinetic barrier that separates the native from the aggregates. (wisc.edu)
  • Huntingtin aggregates formed in cells expressing the mutant form of the protein, but tTG was completely excluded from the aggregates. (rupress.org)
  • The abundance and localization of the aggregates remained unaffected when tTG expression levels were significantly increased in the cells. (rupress.org)
  • In addition, large numbers of aggregates formed when a mutant huntingtin construct was transfected into cells that express no detectable tTG. (rupress.org)
  • The presence of intraneuronal aggregates of tau best correlate with the neuronal cell death that is associated with the clinical signs and symptoms of disease ( 2 ). (biorxiv.org)
  • AD is characterized by aggregation of Tau, a microtubule-binding protein that is normally soluble and mainly localized to neuronal axons, but which can form oligomers and higher order amyloid-like aggregates that accumulate in soma and dendrites and eventually lead to neuronal death. (irb.hr)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • This paper reports the incidence of sickle cell diseases, other haemoglobinopathies and haemoglobinopathy carriers over a 12-month period using high performance liquid chromatography as a primary screening method. (who.int)
  • Our researchers investigate the molecule mechanisms of protein aggregation and work on the next generation of therapies against these diseases. (edu.au)
  • Deterioration of either the structure or function of endothelial cells (ECs) results in a variety of cardiovascular diseases. (researchgate.net)
  • An activated endothelium and more permeable blood-brain barrier could result in infiltration of immune cells as seen in other neurological diseases. (medscape.com)
  • Although it has been suggested that α-syn in neurons would have certain roles in synaptic trafficking ( 3 ), it is mostly studied in relation to multiple neurodegenerative diseases, in particular, synucleinopathies because it is a highly amyloidogenic protein, and consequently prone to form aggregation. (bmbreports.org)
  • Our current projects focus on two areas: (1) tumor suppression, metabolism, and autophagy, and (2) protein quality control systems in animal cells and neurodegenerative diseases. (upenn.edu)
  • Cancer encompasses over 100 diseases that occur in most cell types and organs of the human body. (upenn.edu)
  • As a result, these principles may prove to be crucial and ultimately inspire the design of strategies to control deleterious aggregation leading to a variety of deadly diseases, including prion-related, Parkinson's and Alzheimer's disease, as well as Huntington's chorea. (wisc.edu)
  • Cell coding company bit.bio announces an expansion to its product portfolio to accelerate research into neurodegenerative diseases. (yahoo.com)
  • bit.bio's latest cell products provide a scalable source of human cells and will enable scientists to study neurodegenerative diseases in a human context. (yahoo.com)
  • The launch of two new cell products for research and drug discovery in neurodegenerative diseases validates our cell identity coding platform's ability to create and manufacture any human cell type consistently at scale. (yahoo.com)
  • Many strategies for ameliorating age-related diseases have been extensively studied, including calorie restriction through the control of diet and exercise and pharmaceutical treatments targeting specific cells and molecules (Di Daniele et al. (springer.com)
  • p62 Is a common component of cytoplasmic inclusions in protein aggregation diseases. (uzh.ch)
  • Aggregation of the protein tau from a soluble unfolded state to an insoluble β-sheet enriched filamentous structure underlies numerous human neurodegenerative diseases known as tauopathies ( 1 ). (biorxiv.org)
  • Another area of Lubchenko's research involves protein aggregation, a process thought to be responsible for many degenerative diseases, such as sickle cell anemia or Alzheimer's. (uh.edu)
  • As a promising strategy, gene therapy has drawn significant attention, specially in cell therapy and treatment of gene-associated diseases ranging from hemophilia and cancer to autoimmune diseases. (dovepress.com)
  • A widely published researcher, Fairman studies protein polymerizing systems and is working on better understanding the protein aggregation that underlies diseases such as Huntington's, ALS, and Alzheimer's. (haverford.edu)
  • Age-dependent protein aggregation is a conserved phenomenon that is associated with many neurodegenerative diseases, including Alzheimer's disease (AD). (irb.hr)
  • Although the molecular mechanisms of carcinogenesis are not fully understood, involvement of the tumor suppressor p53 is considered crucial, as p53 plays an essential role preventing cancer development by inducing cell cycle arrest and/or apoptosis in response to genotoxic stress [ 4 - 7 ]. (oncotarget.com)
  • Selective expression of the OAP-forming isoform M23-AQP4 (AQP4-OAP) triggered cell shape changes in glioma cells associated with alterations to the F-actin cytoskeleton that affected apoptosis. (houstonmethodist.org)
  • In a paper in the Journal of Lipid Research , Zhenzhen Zhang and colleagues at Northwest A&F University in Shaanxi, China, reported that in a model for apoptosis in fat cells, two microRNAs are important drivers of the programmed cell death pathway. (asbmb.org)
  • Changes in mitochondrial membrane potential (MMP) and Hoechst 33258 staining were used to detect cell apoptosis. (scialert.net)
  • Despite intensive multimodal therapy (chemotherapy and surgery), ovarian cancer still has a poor prognosis tumorigenesis and malignant transformation are caused by overexpression of the cell survival pathway and normal cellular senescence or apoptosis. (scialert.net)
  • Stress-induced amyloid aggregation in mammalian cells. (sfu.ca)
  • Specialized innate immune cells, like macrophages, have the ability to engulf and kill microbes and mammalian cells alike trough phagocytosis. (sfu.ca)
  • Notch signaling dictates cell fate decisions in mammalian cells including megakaryocytes. (elifesciences.org)
  • High-throughput mapping of a dynamic signaling network in mammalian cells. (nih.gov)
  • The Integrative BMB track will provide you with a broad eduction in Biochemistry and Molecular Biology using an interdisciplinary training in methods and multiple model organisms such as plants, fungi, human /mammalian cells, rodents & nematodes. (uni-bremen.de)
  • Cell aggregation is a complex behavior that is closely associated to the differentiation, migration, and viability of biological cells. (purdue.edu)
  • By contrast to cell stimulation, SMT has not been employed to follow dynamic TF changes along stages of cell differentiation. (uni-ulm.de)
  • Herein, we analysed the serum response factor (SRF), a TF involved in the differentiation of many cell types to study nuclear single-molecule dynamics in neuronal differentiation. (uni-ulm.de)
  • In summary, SMT revealed modulation of dynamic TF properties during cell stimulation and differentiation. (uni-ulm.de)
  • Cell Death & Differentiation, 27 (10). (whiterose.ac.uk)
  • the EST, which assesses the effects of compounds on the differentiation of mouse embryonic stem cells into contracting cardiomyocytes, can be used to rank the potency of chemicals within a series of alkoxyacetic acid metabolites formed from The implementation of the European REACH (Registration, glycol ethers. (cdc.gov)
  • GJB1- encoded GJB1 protein, also named connexin-32 (Cx32), is widely expressed in peripheral myelin and is specifically located at uncompacted folds of Schwann cell cytoplasm around the nodes of Ranvier and Schmidt-Lanterman incisures ( Abrams and Freidin, 2015 ). (frontiersin.org)
  • However, at low DNA concentration, such as the few copies of viral DNA in the cytoplasm of an infected cell, the DNA bridging property of BAF results in intracellular compaction. (nih.gov)
  • This work provides insights into the aggregation of TDP-43 in living cells and provide evidence suggesting that TDP-43 oligomerization and aggregation takes place in the cytoplasm separate from SGs. (uni-ulm.de)
  • Figure 436 from Chapter 16 (Cytoplasmic matrix and cytoskeleton) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Cytoplasm of a Sertoli cell from testis of the ground squirrel, Citellus lateralis in culture. (cellimagelibrary.org)
  • IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. (fishersci.com)
  • We mixed lifetime and phasor FLIM evaluation to judge the affect of human epidermal progress issue receptor 2 (HER2) inhibition, a prevalent most cancers biomarker, on each nuclear and cytoplasmic NAD(P)H of two squamous cell carcinoma (SCC) cultures. (ncbcs.org)
  • Cells have been challenged with mitochondrial inhibition and uncoupling to research AG825's influence on the general metabolic capability. (ncbcs.org)
  • NAD(P)H fluorescence lifetime and certain fraction constantly decreased following HER2 inhibition in each cell strains. (ncbcs.org)
  • Using each, complementary FLIM evaluation strategies along with quantitative fluorescence depth revealed constant, quantitative adjustments in NAD(P)H metabolism related to inhibition of progress issue signaling in SCC cell strains. (ncbcs.org)
  • The Gram-negative bacterium Escherichia coli is one of the model organisms for studying both cell aggregation and biofilm formation. (nature.com)
  • Dissemination of these plasmids among bacterial cells can enhance biofilm formation, biofilm development, and can strengthen the stability of the biofilm structure. (kenyon.edu)
  • IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. (fishersci.com)
  • Platelet aggregation, granule secretion and platelet-leukocyte interaction were analyzed by lumi-aggregometry and flow cytometry. (elifesciences.org)
  • apocrine gland one whose discharged secretion contains part of the secreting cells. (thefreedictionary.com)
  • By contrast, expression of M1-AQP4 (AQP4-tetramers), which is unable to aggregate into OAP, ameliorated glioma cell invasiveness, improved cell migration, and increased methalloproteinase-9 activity. (houstonmethodist.org)
  • HU prevented both P-selectin-induced TF expression and mixed cell aggregate formation. (nih.gov)
  • The thorough analysis of a therapeutic protein product's propensity to aggregate may be a necessary step in the prevention of a cell-mediated immune response. (biopharminternational.com)
  • Moreover, intracellular aggregation probably occurs in the endoplasmic reticulum compartment rather than the Golgi apparatus. (frontiersin.org)
  • K. pneumoniae pathogenicity relies largely on its ability to escape phagocytosis and intracellular killing by phagocytic cells. (unige.ch)
  • We examined the intracellular localization of Tau in young and aged cells, and in cells under different stress conditions, such as glucose starvation, hyperosmotic stress, elevated temperature and proteotoxic stress caused by a toxic amino acid analogue. (irb.hr)
  • The Abbkine Cell Migration Assay Kit is online! (abbkine.com)
  • We developed an aggregation assay protocol on the hospital's instrument, and a member of the Tecan team helped us to perform some validation experiments to confi rm that we could transfer our technology and methods onto the system. (tecan.com)
  • We then went ahead and purchased our own Spark reader, allowing us to perform the same aggregation assay far more costeff ectively than using a high content imaging platform. (tecan.com)
  • Cell viability was measured by MTT assay. (scialert.net)
  • Comparison of two assay methods for patterns of adherence to HEp-2 cells of Escherichia coli from patients with diarrhea. (cdc.gov)
  • This aminosterol enhances the rate of aggregation by promoting monomer-dependent secondary nucleation, but significantly reduces the toxicity of the resulting oligomers to neuroblastoma cells by inhibiting their binding to the cellular membranes. (lu.se)
  • Bates G. Huntingtin aggregation and toxicity in Huntington's disease. (medlineplus.gov)
  • Our results demonstrate that manipulation of the aggregation propensity of Abeta42 does not simply change the level of toxicity, but can also result in qualitative shifts in the pathology induced in vivo. (arctichealth.org)
  • To evaluate the ability of molecules to interfere with aggregation of hCC while informing about cellular toxicity, we generated cells that produce and secrete WT and L68Q-hCC and have detected high-molecular weight complexes formed from the mutant protein. (opinvisindi.is)
  • WRAP-Cell-size-matters-nano-micro-plastics-drive-marine-phytoplankton-co-aggregation-2022.pdf - Published Version - Requires a PDF viewer. (warwick.ac.uk)
  • CAMBRIDGE, England, September 29, 2022 --( BUSINESS WIRE )--Cell coding company bit.bio has announced an expansion to its product portfolio - ioGlutamatergic Neurons TDP-43 M337V disease model and early access to its ioMicroglia cell product. (yahoo.com)
  • Present investigation deals with estimation of cellular aggregation, generation of ROS and activities of antioxidant enzymes, lysozyme and acetylcholinesterase in the cells of E. carteri under the environmentally realistic concentrations of washing soda. (jostchemical.com)
  • Prolonged treatment of washing soda inhibited the degree of cellular aggregation. (jostchemical.com)
  • Protein misfolding and aberrant aggregation processes that elude cellular maintenance mechanisms can result in major disturbances of cellular processes. (cambridge.org)
  • Cellular tomography reveal that the S-layer is nearly continuous on the cell surface, completed by pentameric defects in the hexagonal lattice. (rcsb.org)
  • The aggregation of IgE anchored to high-affinity F є receptors on rat basophilic leukemia (RBL) cells by multivalent antigens initiates transmembrane signaling and ultimately cellular degranulation. (nau.edu)
  • Its life cycle, comprised of both single-cell and multicellular phases, provides an excellent system for studying the effects of NCL gene deficiency on conserved cellular and developmental processes. (mdpi.com)
  • These results demonstrate roles for self-organization and aggregation in persistence of multi-cellular communities, and also illustrate a role for the techniques of synthetic biology in elucidating fundamental biological principles. (caltech.edu)
  • Biomolecular condensates at the nexus of cellular stress, protein aggregation disease and ageing. (tu-dresden.de)
  • It also helps your synthesize hormones, activates coenzymes needed for your cellular metabolism and helps maintain healthy cell membranes. (livestrong.com)
  • The overall goal of this process is to perform density-based cellular analysis for various medical diagnostic applications by measuring the altered distribution of a cell population or by separating cell types based on their characteristic densities. (jove.com)
  • State-of-the-art, large-scale, single-cell and single-nucleus transcriptomics facilitate the exploration of the cardiac cellular landscape at an unprecedented level, beyond its descriptive features, and can further our understanding of the mechanisms of disease and guide functional studies. (cdc.gov)
  • Protein aggregation is a prominent feature of many neurodegenerative disorders including Parkinson's disease (PD). (nih.gov)
  • Parkinson's disease is a neurodegenerative disease caused by progressive dopamine brain cells loss. (medindia.net)
  • Transient oligomeric species formed during the aggregation process of the 42-residue form of the amyloid-β peptide (Aβ 42 ) are key pathogenic agents in Alzheimer's disease (AD). (lu.se)
  • Aggregation of the amyloid-beta-42 (Abeta42) peptide in the brain parenchyma is a pathological hallmark of Alzheimer's disease (AD), and the prevention of Abeta aggregation has been proposed as a therapeutic intervention in AD. (arctichealth.org)
  • investigated the ability of ASC specks to promote amyloid beta aggregation and spreading, linking inflammasome activity with Alzheimer's disease progression. (biolegend.com)
  • Tau aggregation and hyperphosphorylation is a key neuropathological hallmark of Alzheimer's disease (AD), and the temporospatial spread of Tau observed during clinical manifestation suggests that Tau pathology may spread along the axonal network and propagate between synaptically connected neurons. (jbc.org)
  • The underlying aggregation mechanism of this protein is dominated by a secondary process at mildly acidic pH, as in endosomes and other organelles. (cambridge.org)
  • methods for identifying the whole spectrum of STEC strains The colonization mechanism for the cell invasion is not associated with HUS. (cdc.gov)
  • Conjugation may be another mechanism that bacteria develop to transfer plasmid to the adjacent cells. (kenyon.edu)
  • From these findings they concluded that the mechanism by which capsaicin causes prostate cancer cells to die had been identified. (naturalnews.com)
  • The mechanism of cell death in tauopathy remains unclear, however several studies implicate microglia in non-cell-autonomous routes ( 3 - 8 ). (biorxiv.org)
  • In HL-60 cells, internalization is very fast, independent of binding to the cell surface, and proceeds from the fluid phase via a classical clathrin-dependent mechanism, ending up in an endolysosomal compartment from which it is not further released. (ipfdd.de)
  • This substantial difference in internalization rate and mechanism between two cell types has important repercussions for potential application of this class of glycodendrimers as drug delivery agents. (ipfdd.de)
  • Therefore, salmon calcitonin (sCT), which has much lower aggregation propensity but only shares 50% homology to hCT, is clinically used instead 25 . (nature.com)
  • In order to gain more insight into the tumour-host relationship, the present study was conducted to characterize the in situ phenotype of the lymphocytic infiltrate surrounding tumour tissue of BCC through the use of monoclonal antibodies against B and T cell populations. (who.int)
  • Direct immunofluorescent studies with fluorescein-conjugated antineutrophil IgG revealed that the antibodies were cleared by aggregation and endocytosis. (jci.org)
  • The cells are infected with a bacterial strain, then after a series of washes, the eukaryotic cells are stained with DAPI and the bacteria with specifi c antibodies conjugated to an AlexaFluor® dye. (tecan.com)
  • The group's research involves the systematic integration of model-based tools, such as sensitivity analysis, design of experiments and optimisation, with experimentation on mammalian cell culture systems with a focus on metabolism and protein glycosylation. (imperial.ac.uk)
  • Mice and flies given the polyamine in their diet have increased brain cell metabolism and cognitive function, and epidemiological data hints at a similar benefit in humans. (the-scientist.com)
  • A recent extension of this research area is to define the role of metabolism and autophagy in stem cells, including embryonic stem cells and cancer stem cells. (upenn.edu)
  • Cell Metabolism 33(1): 94-109, January 2021. (upenn.edu)
  • Cell structure and metabolism== ''Lactobacillus gasseri'' is rod shaped and a gram-positive bacterium. (kenyon.edu)
  • This correlates with the hydrogen peroxide produced, as described in the Cell Structure and Metabolism section. (kenyon.edu)
  • The underlying pathology leading to loss of color, night, central, or peripheral vision can be accounted for by mutations in specific genes (see RetNet ) known to disrupt crucial processes such as phototransduction, phagocytosis, vitamin A metabolism, cell-to-cell signaling, or gene regulation. (molvis.org)
  • As promised at the end of last year, LTE pioneer SK Telecom has successfully developed LTE-Advanced tri-band carrier aggregation technology, to ease network congestion in built up areas in South Korea. (telecoms.com)
  • Carrier aggregation is a technique that is used in wireless communication to increase the data rate per user, whereby multiple frequency blocks (called component carriers) are assigned to the same user. (wikipedia.org)
  • In addition load balancing: p.42 is possible with carrier aggregation. (wikipedia.org)
  • p. 113 The case where the component carriers are contiguous in the same frequency band is called intra-band contiguous carrier aggregation. (wikipedia.org)
  • If the component carriers are in the same frequency band but are separated by a gap the carrier aggregation is called intra-band non-contiguous. (wikipedia.org)
  • This is called inter-band carrier aggregation applied to heterogeneous networks. (wikipedia.org)
  • However, the complexity from an RF point of view is increased in the case inter-band carrier aggregation. (wikipedia.org)
  • Carrier aggregation is also called Dual Cell in the context of UMTS/HSPA+. (wikipedia.org)
  • Through carrier aggregation (part of the UMTS extension HSPA+) two downlink carriers may be assigned to one user since Release 8. (wikipedia.org)
  • Release 10 supports four-carrier aggregation and eight-carrier-aggregation is supported since Release 11. (wikipedia.org)
  • 3GPP standardized carrier aggregation for HSPA+ for the uplink for up to two component carriers since Release 9. (wikipedia.org)
  • p.30 A difference between two aggregated 10 MHz component carriers and a single ordinary 20 MHz channel is that in the case of carrier aggregation the control information is transmitted on both component carriers. (wikipedia.org)
  • LTE Advanced with carrier aggregation allows Gigabit LTE. (wikipedia.org)
  • This is made possible through higher-order modulation (256QAM), carrier aggregation and 4x4 MIMO. (wikipedia.org)
  • S. C. G. Tsinos, F. Foukalas, T. Khattab and L. Lai "On Channel Selection for Carrier Aggregation Systems. (wikipedia.org)
  • F. Foukalas and T. Tsiftsis, "Energy Efficient Power Allocation for Carrier Aggregation in Heterogeneous Networks: Partial Feedback and Circuit Power Consumption. (wikipedia.org)
  • In addition, they are looking to emulate the success of carrier aggregation (CA) in LTE networks, by introducing carrier aggregation technology to increase the throughput of the 5G services they can provide to their customers. (gsacom.com)
  • operators are expecting carrier aggregation will deliver Gigabit+ 5G networks. (gsacom.com)
  • 5G carrier aggregation also has the potential to increase cell coverage for 5G deployments. (gsacom.com)
  • Currently in use is multiple carrier aggregation and beamforming. (phonearena.com)
  • Our teams are working hard to roll out higher order and Massive MIMO, 256 QAM, Gigabit Class LTE, and four-channel (and later five-channel) carrier aggregation. (phonearena.com)
  • The SkyFlux UAR Disaggregated Router platform applies the network disaggregation concept onto carrier-grade modular redundant router platforms optimized for telecommunications networks, resulting in an open, flexible, and future-proof solution that is able to meet the requirements of telecom applications including 5G backhaul/midhaul, broadband aggregation, metro access and aggregation networks. (utstar.com)
  • It was recognized for LTE Innovation of the Year (LTE-Advanced FDD+TDD Convergence Carrier Aggregation solution), CEM Innovation of the Year (Customer Experience Transformation Center (CETC)) and Data Center Innovation of the Year (Container DC solution). (telecomasia.net)
  • T. Colin , M.-C. Durrieu , J. Joie , Y. Lei , Y. Mammeri , C. Poignard and O. Saut , Modeling of the migration of endothelial cells on bioactive micro-patterned polymers, Mathematical Biosciences and Engineering , 10 (2013) , 997-1015. (aimsciences.org)
  • Redefining cell migration assays. (abbkine.com)
  • A recent study investigating capsaicin on highly metastatic melanoma cells found its anti-mutagenic activity inhibited the migration of melanoma cells at low doses without showing obvious cytotoxicity. (naturalnews.com)
  • Our study provides evidence that resveratrol directly modulates p53, enhancing our understanding of the mechanisms involved in p53 aggregation and its potential as a therapeutic strategy for cancer treatment. (oncotarget.com)
  • Rendering and modulating such a dynamic artificial cell system require mechanisms for receiving, transducing, and transmitting intercellular signals, yet effective tools are limited at present. (purdue.edu)
  • However, the molecular mechanisms underlying leukocyte cell mechanics are poorly understood. (sfu.ca)
  • The Yerbury lab is dedicated to understanding the molecular mechanisms underpinning Motor Neurone Disease (MND), with a particular focus on protein misfolding and protein aggregation. (edu.au)
  • The CCLE was a useful tool since the essential mechanisms involved in the development of AD pathology are conserved across different cell lines. (broadinstitute.org)
  • Loss of function (LoF), dominant negative (DN) and gain of function (GoF) mutations in p53 are associated with amyloid aggregation. (oncotarget.com)
  • Gain-of-function mutations in CARD11 promote enhanced aggregation and idiosyncratic signalosome assembly. (invivogen.com)
  • Phg1A KO D. discoideum cells cannot feed upon K. pneumoniae bacteria, unless bacteria bear mutations decreasing their virulence. (unige.ch)
  • Although the exact function of this protein is unknown, it appears to play an important role in nerve cells (neurons) in the brain and is essential for normal development before birth. (medlineplus.gov)
  • The elongated protein is cut into smaller, toxic fragments that bind together and accumulate in neurons, disrupting the normal functions of these cells. (medlineplus.gov)
  • [ 1 ] These infiltrates were present almost exclusively in the perivascular space and not in the brain parenchyma, indicating indirect effects, if any, on cells like neurons and glia. (medscape.com)
  • Our lab recently identified a novel protein quality control (PQC) system in animal cells that is mechanistically distinct from canonical PQC systems, and is multifunctional and effective in suppressing protein misfolding and aggregation in neurons and other cells. (upenn.edu)
  • The disease model cells and the genetically matched control, ioGlutamatergic Neurons, mature rapidly, are highly reproducible between batches, and have unprecedented scalability. (yahoo.com)
  • Chemical synapses are special cell-cell junctions which facilitate the transmission of neural signal within neurons or from a neuron to target cells. (uni-ulm.de)
  • In this study, we carefully analyzed the subcellular localization of endogenous FUS at synaptic sites of hippocampal neurons which are among the most affected cell types in FTD with FUS pathology. (uni-ulm.de)
  • Platelets are circulating blood cells having central role in hemostasis and pathological thrombus formation that can lead to serious vaso-occlusive pathologies like myocardial infarction and ischemic stroke. (elifesciences.org)
  • Vitamin K affects small blood cells, called platelets, which your body relies on to control bleeding. (livestrong.com)
  • Under a magnification of 1000X, this photomicrograph of a fixed blood smear, revealed a round aggregation of platelets (arrow), a polymorphonuclear leukocyte (PMN), and a number of red blood cells (RBCs). (cdc.gov)
  • What about on platelets, adrenergic nerver terminals, vascular smooth muscle and fat cells? (freezingblue.com)
  • The fact that aggregation is "dynamic, that it is a mixture in solution, and that species do not attach to each other permanently, but through bonds that can be broken by ionic processes, means that we need to rely on both separation and detection," which makes it especially difficult. (biopharminternational.com)
  • Only a few species and few cells were found in the Skagerrak waters at the ordinary phytoplankton stations. (smhi.se)
  • Each genus is made up by aggregation of species. (free-translator.com)
  • Aggregation of sponge cells: the aggregation factor as a tool to establish species. (vliz.be)
  • You will use modern molecular biology techniques, including CRISPR/Cas9 gene editing, to manipulate putative molecular determinants of leukocyte cell mechanics. (sfu.ca)
  • We think, by binding to amyloid fibrils, these molecular chaperones protect the cell from their toxic effects. (edu.au)
  • Disruption in redox homeostasis can result in undesirable molecular interactions throughout the cell. (hindawi.com)
  • Molecular biology of the cell. (elsevier.com)
  • 1990. Cell replication and unscheduled DNA synthesis (UDS) activity of low molecular weight chlorinated paraffins in the rat liver in vivo. (cdc.gov)
  • Rnq1PD shows a different aggregation pattern in [ pin− ] and [ PIN+ ] cells. (elifesciences.org)
  • PDF] O(2) release from erythrocytes flowing in a narrow O(2)-permeable tube: effects of erythrocyte aggregation. (semanticscholar.org)
  • The hemorheological parameters, erythrocyte aggregation and deformability are determined in leprotic patients and are compared with that of healthy subjects. (who.int)
  • The type of bucket aggregation determines whether a given document falls into a bucket or not. (opensearch.org)
  • A bucket aggregation returning a form of adjacency matrix . (elastic.co)
  • There are currently nine known prostaglandin receptors on various cell types. (wikidoc.org)
  • Signaling is induced through binding of five independent ligands, Delta-like ligands (DLL)-1, 3, 4 and Jagged (Jag)-1 and -2 ( Kopan and Ilagan, 2009 ), to four isoforms of cognate Notch receptors, Notch1 to Notch4, on surface of adjacent cells. (elifesciences.org)
  • In order to colonize and initiate an infection, bacteria adhere to host cells via the fi mbriae (hair-like structures) that interact with specifi c host cell receptors. (tecan.com)
  • He followed this up with Brawl in Cell Block 99 (2017) and Dragged Across Concrete (2018), all of which he wrote and composed the music for. (wikipedia.org)
  • In this study, these two compounds were tested for their effects on hCT aggregation. (nature.com)
  • Currently, we are developing a powerful high-throughput microscopy method to screen novel and clinically-approved compounds for their protective properties against MND-related protein aggregation in cultured cells. (edu.au)
  • We identified 3 non-antibiotic compounds that restored growth of phg1A KO cells on K. pneumoniae , and we characterized the mode of action of one of them, 5-ethyl-2'-deoxyuridine (K2). (unige.ch)
  • They found that the compounds restored the health of the cell and resulted in reduction of protein aggregation and protection against misfolding. (medindia.net)
  • To investigate the relationship between Aβ 42 aggregation and its cytotoxicity and the influence of a potential drug on both phenomena, we have studied the effects of trodusquemine. (lu.se)
  • Lara S , Heilig J , Virtanen A , Kleinau S . Exploring complement-dependent cytotoxicity by rituximab isotypes in 2D and 3D-cultured B-cell lymphoma. (wjgnet.com)
  • Aggregation of these molecules leads to bilayer structures such as vesicles, as presented in B2 and B3. (sciepub.com)
  • The new Northwestern research identifies new genes and pathways that prevent protein misfolding and toxic aggregation, keeping cells healthy, and also identifies small molecules with therapeutic potential that restore health to damaged cells, providing new targets for drug development. (medindia.net)
  • In the Nature Chemical Biology study, Morimoto and his colleagues screened nearly one million small molecules in human tissue culture cells to identify those that restore the cell's ability to protect itself from protein damage. (medindia.net)
  • Since joining the Haverford faculty in 1997, Professor of Biology Rob Fairman has taught a wide array of courses, including "Protein Folding and Design," "Molecules, Cells, and Organisms," and "Genetic Roil and Royal Families," which looks at the biology of inherited conditions through the lens of royal intermarriage. (haverford.edu)
  • A micrograph of fat cells stained with Oil Red O shows their lipid vesicle contents. (asbmb.org)
  • Strikingly, prevention of DLL-4-Notch1 interaction by a blocking antibody abolished platelet aggregation and extracellular vesicle shedding induced by thrombin. (elifesciences.org)
  • Additionally, the GAI peptide did not affect GAPDH glycolytic activity or cell viability. (cheric.org)
  • The role of AQP4 aggregation into OAP in malignant gliomas is still unclear. (houstonmethodist.org)
  • Whether MRP4 is the only transporter releasing prostaglandins from the cells is still unclear. (wikidoc.org)
  • However, the pathological significance of GAPDH aggregation in disease pathogenesis remains unclear due to the lack of an effective GAPDH aggregation inhibitor. (cheric.org)
  • Although the main risk factor for the onset of AD is aging, the exact causes of Tau protein aggregation are still largely unclear. (irb.hr)
  • We collaborated with researchers from Egypt, Syrian Arab Republic and Jordan in a study of patients with sickle-cell disease from those countries, and from various parts of Saudi Arabia, in order to investigate the influence of genetics on the clinical presentation of the disease, and to attempt to determine the origin of the sickle-cell gene in Arabs. (who.int)
  • This cell-cell attachment is based on plasmid-encoded and is subject to conjugal gene transfer. (kenyon.edu)
  • 4 Some SSRIs seem to have the ability to regulate cytokine and gene expression in both cell and animal models of inflammation and modulate immune responses. (nursingcenter.com)
  • They reduced expression of each gene one at a time and looked to see if the gene suppressed protein aggregation in the cell. (medindia.net)
  • Did the gene increase aggregation or lessen it or have no effect at all? (medindia.net)
  • This database contains valuable gene expression information for a large number of immortalized cancer cell lines. (broadinstitute.org)
  • PEAR1 is a platelet receptor that signals upon the formation of platelet-platelet contacts independent of platelet activation and secondary to platelet aggregation (Nanda et al. (nih.gov)
  • These results are discussed in terms of recent theoretical developments concerning effects of receptor density on ligand binding to cell surfaces. (nau.edu)
  • A chemical that binds to a receptor on a cell and triggers a response by that cell. (michaeljfox.org)
  • Failure to clear Aβ plaques compromises brain homeostasis and has been linked to genetic variations of the triggering receptor expressed on myeloid cells 2, or TREM-2. (broadinstitute.org)
  • Cells also communicate between themselves to initiate antibiotic biosynthesis, and extracellular enzyme biosynthesis. (kenyon.edu)
  • The extracellular domain possesses a platelet aggregation stimulating (PLAG) domain. (sigmaaldrich.com)
  • of tumor cells induced platelet aggregation (TCIPA). (tci-thaijo.org)
  • In this study, we developed microwell arrays and micropillar electrode arrays that can generate electro-rotation as basic technologies (devices) for detecting circulating tumor cells. (nii.ac.jp)
  • Aggregation typically occurs because of nucleation, surface-induced factors, as a result of conformationally or chemically altered monomers, or association of native monomers (2). (biopharminternational.com)
  • This study shows a new perspective on the role of AQP4 in brain tumors not necessarily associated with edema formation but with AQP4 aggregation/disaggregation dynamics and their link with the actin cytoskeleton. (houstonmethodist.org)
  • Therefore the main objective of this action was to visualise the DNA-uptake pilus in live cells and investigate its dynamics. (europa.eu)
  • The research carried out in the Cavagnero group focuses on understanding protein folding dynamics and aggregation in the cell. (wisc.edu)
  • Tau aggregation underlies neurodegenerative tauopathies, and transcellular propagation of tau assemblies of unique structure, i.e., strains, may underlie the diversity of these disorders. (jbc.org)
  • In 2006, a team of researchers from the Samuel Oschin Comprehensive Cancer Institute at Cedars-Sinai Medical Center, found that capsaicin induced 80 percent of human prostate cancer cells growing in mice to follow pathways leading to certain death. (naturalnews.com)
  • If successful, homeostasis is restored and protein synthesis resumes, but if ER stress persists, cell death pathways are activated. (bmj.com)
  • Such intercellular signalling, known as quorum sensing, allows bacteria to coordinate and synchronize behavioural responses at high cell densities. (nature.com)
  • Once bacteria irreversibly attach to the surface, they produce EPS, which helps bind cells together, to the surface, and to other particulate materials. (kenyon.edu)
  • To transport DNA into the cell, competent bacteria use type IV pili, a widespread and versatile class of cell surface polymers. (europa.eu)
  • We have demonstrated that the polyphenol (-)-epi-gallocatechine gallate (EGCG) inhibited SNCA aggregation, which made it a candidate for therapeutic intervention in PD. (nih.gov)
  • Cabozantinib Combined With Atezolizumab for Advanced RCC This novel therapeutic combination shows encouraging results in patients with advanced renal cell carcinoma. (medscape.com)
  • R. M. Mège , J. Gavard and M. Lambert , Regulation of cell-cell junctions by the cytoskeleton, Current Opinion in Cell Biology , 18 (2006) , 541-548. (aimsciences.org)
  • The goal of this project is to determine the role of specific membrane and cytoskeleton components in leukocyte cell mechanics. (sfu.ca)
  • We analyzed TDP-43 mobility and localization under different stress and recovery conditions using live cell single-molecule tracking and super-resolution microscopy. (uni-ulm.de)
  • Of late, my group has been involved in developing novel techniques to study heat-shock chaperone function in cells and a flow cytometry-based method to count and physically isolate protein inclusions from cells. (edu.au)
  • Chemotaxis to self-secreted attractants is well known to promote aggregation of eukaryotic organisms, such as social amoebae 15 . (nature.com)
  • Our results show basal levels of Tau-NanoBiT reporter signal in logarithmically growing wild-type cells, suggesting that Tau oligomerization does not occur under normal growth conditions. (irb.hr)
  • Expression of FLAG-tagged GJB1 at various time points after transfection revealed evident protein aggregation with both wild-type and mutant forms, indicated with immunostaining and immunoblotting. (frontiersin.org)