CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase: An enzyme that catalyzes the formation of PHOSPHATIDYLINOSITOL and CMP from CDP-DIACYLGLYCEROL and MYOINOSITOL.Transferases (Other Substituted Phosphate Groups): A class of enzymes that transfers substituted phosphate groups. EC 2.7.8.Phosphotransferases: A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.Cytidine Diphosphate Diglycerides: The ester of diacylglycerol with the terminal phosphate of cytidine diphosphate. It serves as an intermediate in the biosynthesis of phosphatidylethanolamine and phosphatidylserine in bacteria.CDPdiacylglycerol-Serine O-Phosphatidyltransferase: An enzyme that catalyzes the formation of phosphatidylserine and CMP from CDPdiglyceride plus serine. EC 2.7.8.8.Diacylglycerol Kinase: An enzyme of the transferase class that uses ATP to catalyze the phosphorylation of diacylglycerol to a phosphatidate. EC 2.7.1.107.Inositol: An isomer of glucose that has traditionally been considered to be a B vitamin although it has an uncertain status as a vitamin and a deficiency syndrome has not been identified in man. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1379) Inositol phospholipids are important in signal transduction.Inositol Phosphates: Phosphoric acid esters of inositol. They include mono- and polyphosphoric acid esters, with the exception of inositol hexaphosphate which is PHYTIC ACID.DiglyceridesInositol 1,4,5-Trisphosphate: Intracellular messenger formed by the action of phospholipase C on phosphatidylinositol 4,5-bisphosphate, which is one of the phospholipids that make up the cell membrane. Inositol 1,4,5-trisphosphate is released into the cytoplasm where it releases calcium ions from internal stores within the cell's endoplasmic reticulum. These calcium ions stimulate the activity of B kinase or calmodulin.Diacylglycerol O-Acyltransferase: An enzyme that catalyses the last step of the TRIACYLGLYCEROL synthesis reaction in which diacylglycerol is covalently joined to LONG-CHAIN ACYL COA to form triglyceride. It was formerly categorized as EC 2.3.1.124.Inositol 1,4,5-Trisphosphate Receptors: Intracellular receptors that bind to INOSITOL 1,4,5-TRISPHOSPHATE and play an important role in its intracellular signaling. Inositol 1,4,5-trisphosphate receptors are calcium channels that release CALCIUM in response to increased levels of inositol 1,4,5-trisphosphate in the CYTOPLASM.Glycerides: GLYCEROL esterified with FATTY ACIDS.Phosphatidylinositols: Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to the hexahydroxy alcohol, myo-inositol. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid, myo-inositol, and 2 moles of fatty acids.Sugar PhosphatesPhosphatidic Acids: Fatty acid derivatives of glycerophosphates. They are composed of glycerol bound in ester linkage with 1 mole of phosphoric acid at the terminal 3-hydroxyl group and with 2 moles of fatty acids at the other two hydroxyl groups.Type C Phospholipases: A subclass of phospholipases that hydrolyze the phosphoester bond found in the third position of GLYCEROPHOSPHOLIPIDS. Although the singular term phospholipase C specifically refers to an enzyme that catalyzes the hydrolysis of PHOSPHATIDYLCHOLINE (EC 3.1.4.3), it is commonly used in the literature to refer to broad variety of enzymes that specifically catalyze the hydrolysis of PHOSPHATIDYLINOSITOLS.Ice Cream: A frozen dairy food made from cream or butterfat, milk, sugar, and flavorings. Frozen custard and French-type ice creams also contain eggs.Metabolome: The dynamic collection of metabolites which represent a cell's or organism's net metabolic response to current conditions.Food Additives: Substances which are of little or no nutritive value, but are used in the processing or storage of foods or animal feed, especially in the developed countries; includes ANTIOXIDANTS; FOOD PRESERVATIVES; FOOD COLORING AGENTS; FLAVORING AGENTS; ANTI-INFECTIVE AGENTS (both plain and LOCAL); VEHICLES; EXCIPIENTS and other similarly used substances. Many of the same substances are PHARMACEUTIC AIDS when added to pharmaceuticals rather than to foods.Metabolomics: The systematic identification and quantitation of all the metabolic products of a cell, tissue, organ, or organism under varying conditions. The METABOLOME of a cell or organism is a dynamic collection of metabolites which represent its net response to current conditions.Myristic Acid: A saturated 14-carbon fatty acid occurring in most animal and vegetable fats, particularly butterfat and coconut, palm, and nutmeg oils. It is used to synthesize flavor and as an ingredient in soaps and cosmetics. (From Dorland, 28th ed)Margarine: A butterlike product made of refined vegetable oils, sometimes blended with animal fats, and emulsified usually with water or milk. It is used as a butter substitute. (From Random House Unabridged Dictionary, 2d ed)Carbon-Sulfur Ligases: Enzymes that catalyze the joining of two molecules by the formation of a carbon-sulfur bond. EC 6.2.Acyl Carrier Protein: Consists of a polypeptide chain and 4'-phosphopantetheine linked to a serine residue by a phosphodiester bond. Acyl groups are bound as thiol esters to the pantothenyl group. Acyl carrier protein is involved in every step of fatty acid synthesis by the cytoplasmic system.Thiolester HydrolasesAcyltransferases: Enzymes from the transferase class that catalyze the transfer of acyl groups from donor to acceptor, forming either esters or amides. (From Enzyme Nomenclature 1992) EC 2.3.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Spectrometry, Mass, Fast Atom Bombardment: A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. Positive and negative fast atom bombardment spectra are recorded on a mass spectrometer fitted with an atom gun with xenon as the customary beam. The mass spectra obtained contain molecular weight recognition as well as sequence information.Mycobacterium: A genus of gram-positive, aerobic bacteria. Most species are free-living in soil and water, but the major habitat for some is the diseased tissue of warm-blooded hosts.Glycerophospholipids: Derivatives of phosphatidic acid in which the hydrophobic regions are composed of two fatty acids and a polar alcohol is joined to the C-3 position of glycerol through a phosphodiester bond. They are named according to their polar head groups, such as phosphatidylcholine and phosphatidylethanolamine.Glycerol: A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, and sweetening agent.Sphingolipids: A class of membrane lipids that have a polar head and two nonpolar tails. They are composed of one molecule of the long-chain amino alcohol sphingosine (4-sphingenine) or one of its derivatives, one molecule of a long-chain acid, a polar head alcohol and sometimes phosphoric acid in diester linkage at the polar head group. (Lehninger et al, Principles of Biochemistry, 2nd ed)Fatty Acids: Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)Phospholipids: Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.Sarcoma, Kaposi: A multicentric, malignant neoplastic vascular proliferation characterized by the development of bluish-red cutaneous nodules, usually on the lower extremities, most often on the toes or feet, and slowly increasing in size and number and spreading to more proximal areas. The tumors have endothelium-lined channels and vascular spaces admixed with variably sized aggregates of spindle-shaped cells, and often remain confined to the skin and subcutaneous tissue, but widespread visceral involvement may occur. Kaposi's sarcoma occurs spontaneously in Jewish and Italian males in Europe and the United States. An aggressive variant in young children is endemic in some areas of Africa. A third form occurs in about 0.04% of kidney transplant patients. There is also a high incidence in AIDS patients. (From Dorland, 27th ed & Holland et al., Cancer Medicine, 3d ed, pp2105-7) HHV-8 is the suspected cause.Lymphoma, Primary Effusion: A rare neoplasm of large B-cells usually presenting as serious effusions without detectable tumor masses. The most common sites of involvement are the pleural, pericardial, and peritoneal cavities. It is associated with HUMAN HERPESVIRUS 8, most often occurring in the setting of immunodeficiency.Herpesvirus 8, Human: A species in the genus RHADINOVIRUS, subfamily GAMMAHERPESVIRINAE, isolated from patients with AIDS-related and "classical" Kaposi sarcoma.Acquired Immunodeficiency Syndrome: An acquired defect of cellular immunity associated with infection by the human immunodeficiency virus (HIV), a CD4-positive T-lymphocyte count under 200 cells/microliter or less than 14% of total lymphocytes, and increased susceptibility to opportunistic infections and malignant neoplasms. Clinical manifestations also include emaciation (wasting) and dementia. These elements reflect criteria for AIDS as defined by the CDC in 1993.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Pyridoxal Kinase: An enzyme that catalyzes reversibly the phosphorylation of pyridoxal in the presence of ATP with the formation of pyridoxal 5-phosphate and ADP. Pyridoxine, pyridoxamine and various derivatives can also act as acceptors. EC 2.7.1.35.Fructokinases: A class of enzymes that catalyzes the phosphorylation of fructose in the presence of ATP. EC 2.7.1.-.Pantetheine: An intermediate in the pathway of coenzyme A formation in mammalian liver and some microorganisms.GluconatesErythritol: A four-carbon sugar that is found in algae, fungi, and lichens. It is twice as sweet as sucrose and can be used as a coronary vasodilator.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.cdc25 Phosphatases: A subclass of dual specificity phosphatases that play a role in the progression of the CELL CYCLE. They dephosphorylate and activate CYCLIN-DEPENDENT KINASES.cdc42 GTP-Binding Protein: A member of the Rho family of MONOMERIC GTP-BINDING PROTEINS. It is associated with a diverse array of cellular functions including cytoskeletal changes, filopodia formation and transport through the GOLGI APPARATUS. This enzyme was formerly listed as EC 3.6.1.47.CDC2 Protein Kinase: Phosphoprotein with protein kinase activity that functions in the G2/M phase transition of the CELL CYCLE. It is the catalytic subunit of the MATURATION-PROMOTING FACTOR and complexes with both CYCLIN A and CYCLIN B in mammalian cells. The maximal activity of cyclin-dependent kinase 1 is achieved when it is fully dephosphorylated.Heartwater Disease: A tick-borne septicemic disease of domestic and wild ruminants caused by EHRLICHIA RUMINANTIUM.Ehrlichia ruminantium: A species of gram-negative bacteria in the family ANAPLASMATACEAE, that causes HEARTWATER DISEASE in ruminants.Translational Medical Research: The application of discoveries generated by laboratory research and preclinical studies to the development of clinical trials and studies in humans. A second area of translational research concerns enhancing the adoption of best practices.Awards and PrizesNational Institutes of Health (U.S.): An operating division of the US Department of Health and Human Services. It is concerned with the overall planning, promoting, and administering of programs pertaining to health and medical research. Until 1995, it was an agency of the United States PUBLIC HEALTH SERVICE.Political Systems: The units based on political theory and chosen by countries under which their governmental power is organized and administered to their citizens.Democracy: A system of government in which there is free and equal participation by the people in the political decision-making process.C-Reactive Protein: A plasma protein that circulates in increased amounts during inflammation and after tissue damage.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Biological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.Inflammation: A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.Oligonucleotides: Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)Tyrosine: A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.Receptor Protein-Tyrosine Kinases: A class of cellular receptors that have an intrinsic PROTEIN-TYROSINE KINASE activity.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Adenosine Triphosphatases: A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.Protein-Tyrosine Kinases: Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.Myo-Inositol-1-Phosphate Synthase: An enzyme that catalyzes the formation of myo-inositol-1-phosphate from glucose-6-phosphate in the presence of NAD. EC 5.5.1.4.Gene Expression Regulation, Fungal: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.Jehovah's Witnesses: Members of a religious denomination founded in the United States during the late 19th century in which active evangelism is practiced, the imminent approach of the millennium is preached, and war and organized government authority in matters of conscience are strongly opposed (from American Heritage Dictionary of the English Language, 4th ed). Jehovah's Witnesses generally refuse blood transfusions and other blood-based treatments based on religious belief.Trehalase: An enzyme that catalyzes the conversion of alpha,alpha-trehalose and water to D-glucose. EC 3.2.1.28.TrehaloseGlycogenSaccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.Vicia sativa: A plant species of the genus VICIA, family FABACEAE. The seed is used for food and contains THIOCYANATES such as prunasin, cyanoalanine, cyanogen, and vicine.Oryza sativa: Annual cereal grass of the family POACEAE and its edible starchy grain, rice, which is the staple food of roughly one-half of the world's population.District of Columbia: A federal area located between Maryland and Virginia on the Potomac river; it is coextensive with Washington, D.C., which is the capital of the United States.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Pentosan Sulfuric Polyester: A sulfated pentosyl polysaccharide with heparin-like properties.Postpericardiotomy Syndrome: A nonspecific hypersensitivity reaction caused by TRAUMA to the PERICARDIUM, often following PERICARDIOTOMY. It is characterized by PERICARDIAL EFFUSION; high titers of anti-heart antibodies; low-grade FEVER; LETHARGY; loss of APPETITE; or ABDOMINAL PAIN.Postpoliomyelitis Syndrome: A syndrome characterized by new neuromuscular symptoms that occur at least 15 years after clinical stability has been attained in patients with a prior history of symptomatic poliomyelitis. Clinical features include new muscular weakness and atrophy of the limbs, bulbar innervated musculature, and muscles of respiration, combined with excessive fatigue, joint pain, and reduced stamina. The process is marked by slow progression and periods of stabilization. (From Ann NY Acad Sci 1995 May 25;753:68-80)Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Prospective Payment System: A system wherein reimbursement rates are set, for a given period of time, prior to the circumstances giving rise to actual reimbursement claims.

Identification of AtPIS, a phosphatidylinositol synthase from Arabidopsis. (1/56)

Phosphatidylinositol synthase is the enzyme responsible for the synthesis of phosphatidylinositol, a key phospholipid component of all eukaryotic membranes and the precursor of messenger molecules involved in signal transduction pathways for calcium-dependent responses in the cell. Using the amino acid sequence of the yeast enzyme as a probe, we identified an Arabidopsis expressed sequence tag potentially encoding the plant enzyme. Sequencing the entire cDNA confirmed the homology between the two proteins. Functional assays, performed by overexpression of the plant cDNA in Escherichia coli, a bacteria which lacks phosphatidylinositol and phosphatidylinositol synthase activity, showed that the plant protein induced the accumulation of phosphatidylinositol in the bacterial cells. Analysis of the enzymatic activity in vitro showed that synthesis of phosphatidylinositol occurs when CDP-diacylglycerol and myo-inositol only are provided as substrates, that it requires manganese or magnesium ions for activity, and that it is at least in part located to the bacterial membrane fraction. These data allowed us to conclude that the Arabidopsis cDNA codes for a phosphatidylinositol synthase. A single AtPIS genetic locus was found, which we mapped to Arabidopsis chromosome 1.  (+info)

Phosphatidylinositol is an essential phospholipid of mycobacteria. (2/56)

Phosphatidylinositol (PI) and metabolically derived products such as the phosphatidylinositol mannosides and linear and mature branched lipomannan and lipoarabinomannan are prominent phospholipids/lipoglycans of Mycobacterium sp. believed to play important roles in the structure and physiology of the bacterium as well as during host infection. To determine if PI is an essential phospholipid of mycobacteria, we identified the pgsA gene of Mycobacterium tuberculosis encoding the phosphatidylinositol synthase enzyme and constructed a pgsA conditional mutant of Mycobacterium smegmatis. The ability of this mutant to synthesize phosphatidylinositol synthase and subsequently PI was dependent on the presence of a functional copy of the pgsA gene carried on a thermosensitive plasmid. The mutant grew like the control strain under permissive conditions (30 degrees C), but ceased growing when placed at 42 degrees C, a temperature at which the rescue plasmid is lost. Loss of cell viability at 42 degrees C was observed when PI and phosphatidylinositol dimannoside contents dropped to approximately 30 and 50% of the wild-type levels, respectively. This work provides the first evidence of the essentiality of PI to the survival of mycobacteria. PI synthase is thus an essential enzyme of Mycobacterium that shows promise as a drug target for anti-tuberculosis therapy.  (+info)

Molecular cloning, functional complementation in Saccharomyces cerevisiae and enzymatic properties of phosphatidylinositol synthase from the protozoan parasite Toxoplasma gondii. (3/56)

The obligate intracellular parasite Toxoplasma gondii, the causative agent of toxoplasmosis, switches between the rapidly dividing tachyzoite and the slowly replicating bradyzoite in intermediate hosts such as humans and domestic animals. We have recently identified a bradyzoite cDNA encoding a putative phosphatidylinositol (PtdIns) synthase using a subtractive library [Yahiaoui, B., Dzierszinski, F., Bernigaud, A., Slomianny, C., Camus, D., and Tomavo, S. (1999) Mol. Biochem. Parasitol. 99, 223-235]. Here, we report the cloning of another cDNA encoding PtdIns synthase that is exclusively expressed in the tachyzoite stage. The two transcripts are encoded by two different genes, which are stage-specifically regulated. The deduced amino-acid sequence (258 amino acids with a calculated total molecular mass of 27.8 kDa) of the tachyzoite-specific cDNA shares a significant degree of identity (between 26.5 and 30.1%) to the PtdIns synthases from human, rat, Arabidopsis thaliana and yeast. Interestingly, the putative protein encompasses an N-terminal extension that is approximately 40 amino-acids longer than that of PtdIns synthases from other organisms. Functional complementation realized by tetrad analysis of segregants of a Saccharomyces cerevisiae PtdIns synthase-deficient mutant (PIS1/pis1:kanMX4) showed that only the T. gondii putative PtdIns synthase truncated at its N-terminal extension is able to restore the viability of the cells. We demonstrate that this protein expressed in yeast transformants is functionally active in the membrane preparation and requires manganese and magnesium ions for activity. To our knowledge, this is the first report on the molecular cloning and functional analysis of a gene encoding a PtdIns synthase in protozoan parasites.  (+info)

Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis. (4/56)

In order to study some of its enzymatic properties, phosphatidylinositol synthase 1 (AtPIS1) from the plant Arabidopsis thaliana was expressed in Escherichia coli, a host naturally devoid of phosphatidylinositol (PtdIns). In the context of the bacterial membrane and in addition to de novo synthesis, the plant enzyme is capable of catalysing the exchange of the inositol polar head for another inositol. Our data clearly show that the CDP-diacylglycerol-independent exchange reaction can occur using endogenous PtdIns molecular species or PtdIns molecular species from soybean added exogenously. Exchange has been observed in the absence of cytidine monophosphate (CMP), but is greatly enhanced in the presence of 4 microm CMP. Our data also show that AtPIS1 catalyses the removal of the polar head in the presence of much higher concentrations of CMP, in a manner that suggests a reverse of synthesis. All of the PtdIns metabolizing activities require free manganese ions. EDTA, in the presence of low Mn2+ concentrations, also has an enhancing effect.  (+info)

Regulation of CDP-diacylglycerol synthesis and utilization by inositol and choline in Schizosaccharomyces pombe. (5/56)

CDP-diacylglycerol (CDP-DG) is an important branchpoint intermediate in eucaryotic phospholipid biosynthesis and could be a key regulatory site in phospholipid metabolism. Therefore, we examined the effects of growth phase, phospholipid precursors, and the disruption of phosphatidylcholine (PC) synthesis on the membrane-associated phospholipid biosynthetic enzymes CDP-DG synthase, phosphatidylglycerolphosphate (PGP) synthase, phosphatidylinositol (PI) synthase, and phosphatidylserine (PS) synthase in cell extracts of the fission yeast Schizosaccharomyces pombe. In complete synthetic medium containing inositol, maximal expression of CDP-DG synthase, PGP synthase, PI synthase, and PS synthase in wild-type cells occurred in the exponential phase of growth and decreased two- to fourfold in the stationary phase of growth. In cells starved for inositol, this decrease in PGP synthase, PI synthase, and PS synthase expression was not observed. Starvation for inositol resulted in a twofold derepression of PGP synthase and PS synthase expression, while PI synthase expression decreased initially and then remained constant. Upon the addition of inositol to inositol-starved cells, there was a rapid and continued increase in PI synthase expression. We examined expression of these enzymes in cho2 and cho1 mutants, which are blocked in the methylation pathway for synthesis of PC. Choline starvation resulted in a decrease in PS synthase and CDP-DG synthase expression in cho1 but not cho2 cells. Expression of PGP synthase and PI synthase was not affected by choline starvation. Inositol starvation resulted in a 1.7-fold derepression of PGP synthase expression in cho2 but not cho1 cells when PC was synthesized. PS synthase expression was not depressed, while CDP-DG synthase and PI synthase expression decreased in cho2 and cho1 cells in the absence of inositol. These results demonstrate that (i) CDP-DG synthase, PGP synthase, PI synthase, and PS synthase are similarly regulated by growth phase; (ii) inositol affects the expression of PGP synthase, PI synthase, and PS synthase; (iii) disruption of the methylation pathway results in aberrant patterns of regulation of growth phase and phospholipid precursors. Important differences between S. pombe and Saccharomyces cerevisiae with regard to regulation of these enzymes are discussed.  (+info)

Inhibition of CDP-DG: inositol transferase by inostamycin. (6/56)

Inostamycin, a novel microbial secondary metabolite, inhibited [3H]inositol and 32P1 incorporation into phosphatidylinositol (PtdIns) induced by epidermal growth factor (EGF) in cultured A431 cells, the IC50 being 0.5 micrograms/ml, without inhibiting macromolecular synthesis. The drug inhibited cellular inositol phosphate formation only when it was added at the same time as labeled inositol. It was found to inhibit in vitro CDP-DG:inositol transferase activity of the A431 cell membrane, the IC50 being about 0.02 micrograms/ml. It did not inhibit tyrosine kinase, PtdIns phospholipase C, or PtdIns kinase. Therefore, inhibition of PtdIns turnover by inostamycin must be due to the inhibition of CDP-DG:inositol transferase. Thus, inostamycin is a novel inhibitor of CDP-DG:inositol transferase.  (+info)

Evidence of a role for phosphatidylinositol synthesis in human amnion cell proliferation. (7/56)

Phosphatidylinositol (PtdIns) is the key precursor of phosphoinositide-derived intracellular mediators. The effects of changing the rate of PtdIns synthesis on mitogenic activity of human amnion-derived WISH cells were investigated. Incubation of the cells with [3H]inositol caused a time- and dose-dependent PtdIns labeling. Exogenous Ca2+ inhibited [3H]inositol incorporation in a dose-dependent fashion; half-maximal inhibition occurred with 0.3-1.0 mM Ca2+. In contrast, removal of cytosolic Ca2+ by ionophore A23187 and 1 mM EGTA induced enhancement of the PtdIns labeling as a function of A23187 concentration, perhaps through release of inhibitory effects of endogenous Ca2+. The A23187-stimulated PtdIns labeling with [3H]inositol was not abolished by additional unlabeled inositol, suggesting that [3H]inositol labeling of PtdIns occurred mainly through de novo synthesis catalyzed by PtdIns synthase (EC 2.7.8.11). In cells with PtdIns synthase activity decreased by exogenous Ca2+, [3H]thymidine incorporation was also inhibited, while A23187 caused dose-dependent enhancement of thymidine incorporation. The changes in PtdIns synthase activity occurred in parallel with changes in mitogenic activity caused by increasing the dose of exogenous Ca2+ or A23187. A similar lowering of mitogenic activity was observed upon suppression of PtdIns synthase by pemirolast potassium (9-methyl-3-1H-tetrazol-5yl-4H-pyrido[1,2-a]pyridin-4-one potassium) via a Ca(2+)-independent mechanism. These data demonstrate that changes in PtdIns synthase activity by some agents acting via different mechanisms are associated with parallel changes in thymidine incorporation, and suggest that PtdIns production is tightly coupled to cell proliferation in human amnion cells.  (+info)

Synthetic capacity of Arabidopsis phosphatidylinositol synthase 1 expressed in Escherichia coli. (8/56)

Phosphatidylinositol (PtdIns) synthase 1 from the plant Arabidopsis thaliana has been expressed in Escherichia coli in order to study the synthetic capacities of the enzyme. Analysis of the total fatty acid content of the bacteria shows that PtdIns synthase activity does not have a profound effect on the proportions of the different fatty acids produced, even if the presence of an extra acidic phospholipid leads to a global reduction of the lipid content. A closer analysis carried out on individual phospholipids reveals a global fatty acid composition almost unchanged in the two major bacterial lipids phosphatidylethanolamine (PtdEtn) and phosphatidylglycerol (PtdGro). Phosphatidylinositol has a very unusual composition that shows the ability of the plant enzyme to use CDP-diacylglycerol molecular species absent from plants. We identified the various PtdIns molecular species. They represent a pool of the major molecular species of PtdEtn and PtdGro. These results, together with the determination of the apparent affinity constants of AtPIS1 for myo-inositol and CDP-diacylglycerol, allow us to discuss some of the constraints of PtdIns synthesis in plants in terms of specificity, which will depend on the subcellular localization of the protein.  (+info)

*CDP-diacylglycerol-inositol 3-phosphatidyltransferase

CDP-DG:inositol transferase, cytidine diphosphodiglyceride-inositol phosphatidyltransferase, CDP-diacylglycerol:myo-inositol-3- ... CDP-diacylglycerol-inositol phosphatidyltransferase, CDP-diglyceride:inositol transferase, cytidine 5'-diphospho-1,2-diacyl-sn- ... phosphatidyltransferase, CDP-diglyceride-inositol transferase, cytidine diphosphoglyceride-inositol phosphatidyltransferase, ... phosphatidyl-1D-myo-inositol Thus, the two substrates of this enzyme are CDP-diacylglycerol and myo-inositol, whereas its two ...

*CDIPT

CDP-diacylglycerol-inositol 3-phosphatidyltransferase is an enzyme that in humans is encoded by the CDIPT gene. ... Phosphatidylinositol synthase, a member of the CDP-alcohol phosphatidyl transferase class-I family, is an integral membrane ... "Entrez Gene: CDIPT CDP-diacylglycerol--inositol 3-phosphatidyltransferase (phosphatidylinositol synthase)". Human CDIPT genome ... Two enzymes, CDP-diacylglycerol synthase and phosphatidylinositol synthase, are involved in the biosynthesis of ...

*Chromosome 16 (human)

CDP-diacylglycerol-inositol 3-phosphatidyltransferase CFDP1: Craniofacial development protein 1 CHDS1: Coronary heart disease, ... 3 (2): 243-54. PMID 10464676. Martin J, et al. (2004). "The sequence and analysis of duplication-rich human chromosome 16". ... Chromosome 16 spans about 90 million base pairs (the building material of DNA) and represents just under 3% of the total DNA in ... ISBN 978-3-318-02253-7. Sethakulvichai, W.; Manitpornsut, S.; Wiboonrat, M.; Lilakiatsakun, W.; Assawamakin, A.; Tongsima, S. ( ...

*List of MeSH codes (D08)

CDP-diacylglycerol-serine O-phosphatidyltransferase MeSH D08.811.913.696.900.200 --- diacylglycerol cholinephosphotransferase ... CDP-diacylglycerol-inositol 3-phosphatidyltransferase MeSH D08.811.913.696.900.150 --- ... myo-inositol-1-phosphate synthase MeSH D08.811.399.475.200 --- aldose-ketose isomerases MeSH D08.811.399.475.200.174 --- ... phosphatidylinositol diacylglycerol-lyase MeSH D08.811.277.352.640.700.700.750 --- phospholipase c gamma MeSH D08.811.277.352. ...

*List of EC numbers (EC 2)

CDP-diacylglycerol-choline O-phosphatidyltransferase EC 2.7.8.25: triphosphoribosyl-dephospho-CoA synthase EC 2.7.8.26: ... CDP-diacylglycerol-inositol 3-phosphatidyltransferase EC 2.7.8.12: CDP-glycerol glycerophosphotransferase EC 2.7.8.13: phospho- ... CDP-diacylglycerol-serine O-phosphatidyltransferase EC 2.7.8.9: phosphomannan mannosephosphotransferase EC 2.7.8.10: ... CDP-acylglycerol O-arachidonoyltransferase EC 2.3.1.71: glycine N-benzoyltransferase EC 2.3.1.72: indoleacetylglucose-inositol ...
TY - JOUR. T1 - A revised biosynthetic pathway for phosphatidylinositol in Mycobacteria. AU - Morii, Hiroyuki. AU - Ogawa, Midori. AU - Fukuda, Kazumasa. AU - Taniguchi, Hatsumi. AU - Koga, Yosuke. PY - 2010/11/1. Y1 - 2010/11/1. N2 - For the last decade, it has been believed that phosphatidylinositol (PI) in mycobacteria is synthesized from free inositol and CDP-diacylglycerol by PI synthase in the presence of ATP. The role of ATP in this process, however, is not understood. Additionally, the PI synthase activity is extremely low compared with the PI synthase activity of yeast. When CDP-diacylglycerol and [ 14C]1L-myo-inositol 1-phosphate were incubated with the cell wall components of Mycobacterium smegmatis, both phosphatidylinositol phosphate (PIP) and PI were formed, as identified by fast atom bombardment-mass spectrometry and thin-layer chromatography. PI was formed from PIP by incubation with the cell wall components. Thus, mycobacterial PI was synthesized from CDP-diacylglycerol and ...
In an inositol-depleted 1321 N1 astrocytoma cell line, propranolol at 0.5 mM concentration and carbachol in the presence of Li+ induce a large increase (30-60-fold) in the amount of CMP-phosphatidate, the lipid substrate of PtdIns synthase. The actions of both agents on CMP-phosphatidate accumulation were reversed by co-incubation with 1 mM inositol. In cells grown in the presence of 40 microM inositol the propranolol- and carbachol-mediated CMP-phosphatidate accumulation was much smaller (2-4-fold). Propranolol- and carbachol-mediated increases in CMP-phosphatidate accumulation were at least additive in both inositol-replete and -depleted cells. The subcellular distribution of accumulated CMP-phosphatidate was investigated by sucrose-density-gradient centrifugation of a lysate of inositol-depleted cells. There were two coincident peaks of carbachol-stimulated [3H]CMP-phosphatidate and PtdIns synthase activity, respectively. The first peak of accumulated [3H]CMP-phosphatidate and PtdIns synthase ...
CDP-DG(20:0/22:0) belongs to the family of CDP-diacylglycerols. It is a glycerophospholipid containing a diacylglycerol, with a cytidine diphosphate attached to the oxygen O1 or O2 of the glycerol part. As is the case with diacylglycerols, phosphatidylserines can have many different combinations of fatty acids of varying lengths and saturation attached to the C-1 and C-2 positions. CDP-DG(20:0/22:0), in particular, consists of two eicosanoyl chain at positions C-1 and C2. In E. coli glycerophospholipid metabolism, The biosynthesis of CDP-diacylglycerol (CDP-DG) involves condensation of phosphatidic acid (PA) and cytidine triphosphate, with elimination of pyrophosphate, catalysed by the enzyme CDP-diacylglycerol synthase. The resulting CDP-diacylglycerol can be utilized immediately for the synthesis of phosphatidylglycerol (PG), and thence cardiolipin (CL), and of phosphatidylinositol (PI). CDP-DG(20:0/22:0) is also a substrate of CDP-diacylglycerol pyrophosphatase. It is involved in ...
Stimulation of enzyme secretion in the pancreas on injection of a single dose of the cholinergic drug, pilocarpine, was associated with an increased incorporation of [2-3H]myoinositol into a lipid, which was previously characterized as phosphatidylinositol. Stimulation of enzyme secretion by hourly injection of the pancreozymin congener, caerulein, led to more increased phosphatidylinositol synthesis than with a single injection of pilocarpine. The amylase level of the pancreas remained at a low level as long as caerulein was injected, indicating continued stimulation of enzyme secretion even though increased phosphatidylinositol synthesis ceased after 6 h. Feeding gave the same stimulation of phosphatidylinositol synthesis as caerulein. The major synthesis of phosphatidylinositol in controls and the stimulation of phosphatidylinositol synthesis by pilocarpine was entirely confined to the microsome fraction throughout the experiments (up to 18 h). This shows that there is no flow of microsomal ...
Phosphoinositides (PIs) and their derivatives are essential cellular components that form the building blocks for cell membranes and regulate numerous cell functions. Specifically, the ability to generate myo-inositol 1,4,5-trisphosphate (InsP3) via phospholipase C (PLC) dependent hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to InsP3 and diacylglycerol (DAG) initiates intracellular calcium signaling events representing a fundamental signaling mechanism dependent on PIs. InsP3 produced by PI turnover as a second messenger causes intracellular calcium release, especially from endoplasmic reticulum, by binding to the InsP3 receptor (InsP3R). Various PIs and the enzymes, such as phosphatidylinositol synthase and phosphatidylinositol 4-kinase, necessary for their turnover have been characterized in Apicomplexa, a large phylum of mostly commensal organisms that also includes several clinically relevant parasites. However, InsP3Rs have not been identified in genomes of apicomplexans, ...
1GR0: Crystal Structure of Inositol 1-Phosphate Synthase from Mycobacterium Tuberculosis, a Key Enzyme in Phosphatidylinositol Synthesis
Fosfatidat citidililtransferaza (EC 2.7.7.41, CDP digliceridna pirofosforilaza, CDP-diacilglicerolna sintaza, CDP-diacilgliceridna sintetaza, citidin difosfogliceridna pirofosforilaza, fosfatidat citidiltransferaza, fosfatidinsko kiselinska citidililtransferaza, CTP:1,2-diacilglicerofosfat-citidil transferaza, CTP-diacilglicerol sintetaza, DAG sintetaza, CDP-DG) je enzim sa sistematskim imenom CTP:fosfatidat citidililtransferaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju. ...
Clostridium butyricum phosphatidyltransferase: from Clostridium butyricum; catalyzes transfer of the phosphatidyl moiety of phosphatidylethanolamine, phosphatidylglycerol or phosphatidylserine to primary alcohols such as glycerol, serine and ethanolamine; catalyze transfer of both the diacyl and alkenyl acyl forms of glycerophospholipids, but the diacyl forms are used preferentially
The incorporation of [3H]Ins into PtdIns by exchange of free and lipid-bound inositol moieties occurs via the action of at least two types of Mg2+/Mn(2+)-dependent enzymes in turkey erythrocytes. One is a nucleotide-independent PtdIns/Ins exchange enzyme and its function is, as yet, unknown, whereas the other is CMP-dependent and appears to be an exchange reaction catalysed by PtdIns synthase. The effects of analogues with modifications of the substituent at the 1-, 2-, 3-, 4- and 5-positions on the incorporation of [3H]Ins into PtdIns under both synthase and exchange reaction conditions were investigated in turkey erythrocytes. Analogues causing substantial inhibition of [3H]Ins incorporation were then used in kinetic experiments to determine the type of inhibition involved. The analogues 1-deoxy-1-fluoro-scyllo-inositol and 5-O-methyl-myo-inositol exhibited the greatest effects on the incorporation of [3H]Ins via both the synthase and exchange reactions, and the kinetic analysis indicated that ...
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Toxoplasma gondii is among the most prevalent protozoan parasites, which infects a wide range of organisms including one-third of the human population. Its rapid intracellular replication within a vacuole requires efficient synthesis of glycerophospholipids. Cytidine diphosphate-diacylglycerol (CDP-DAG) serves as a major precursor for phospholipid synthesis. Given the peculiarities of lipid biogenesis, understanding the mechanism and physiological importance of CDP-DAG synthesis is particularly relevant in T. gondii Here, we report the occurrence of two phylogenetically divergent CDP-DAG synthase (CDS) enzymes in the parasite. The eukaryotic-type TgCDS1 and the prokaryotic-type TgCDS2 reside in the endoplasmic reticulum (ER) and apicoplast, respectively. Conditional knockdown of TgCDS1 severely attenuated the parasite growth and resulted in a nearly complete loss of virulence in a mouse model. Moreover, mice infected with the TgCDS1 mutant became fully resistant to challenge infection with a ...
CL(i-12:0/i-24:0/i-17:0/i-17:0) is a cardiolipin (CL). Cardiolipins are sometimes called double phospholipids because they have four fatty acid tails, instead of the usual two. They are glycerophospholipids in which the O1 and O3 oxygen atoms of the central glycerol moiety are each linked to one 1,3-diacylglyerol chain. Their general formula is OC(COP(O)(=O)OC[[email protected]@H](CO[R1])O[R2])COP(O)(=O)OC[[email protected]@H](CO[R3])O[R4], where R1-R4 are four fatty acyl chains. CL(i-12:0/i-24:0/i-17:0/i-17:0) contains one chain of 10-methylundecanoic acid at the C1 position, one chain of 22-methyltricosanoic acid at the C2 position, two chains of 15-methylhexadecanoic acid at the C3 and C4 positions fatty acids. Cardiolipins are known to be present in all mammalian cells, especially cells with a high number of mitochondria. De novo synthesis of Cardiolipins begins with condensing phosphatidic acid (PA) with cytidine-5-triphosphate (CTP) to form cytidine-diphosphate-1,2-diacyl-sn-glycerol (CDP-DG). Glycerol-3-phosphate ...
CL(i-12:0/i-22:0/i-22:0/i-21:0) is a cardiolipin (CL). Cardiolipins are sometimes called double phospholipids because they have four fatty acid tails, instead of the usual two. They are glycerophospholipids in which the O1 and O3 oxygen atoms of the central glycerol moiety are each linked to one 1,3-diacylglyerol chain. Their general formula is OC(COP(O)(=O)OC[[email protected]@H](CO[R1])O[R2])COP(O)(=O)OC[[email protected]@H](CO[R3])O[R4], where R1-R4 are four fatty acyl chains. CL(i-12:0/i-22:0/i-22:0/i-21:0) contains one chain of 10-methylundecanoic acid at the C1 position, two chains of 20-methylheneicosanoic acid at the C2 and C3 positions, one chain of 19-methyleicosanoic acid at the C4 position fatty acids. Cardiolipins are known to be present in all mammalian cells, especially cells with a high number of mitochondria. De novo synthesis of Cardiolipins begins with condensing phosphatidic acid (PA) with cytidine-5-triphosphate (CTP) to form cytidine-diphosphate-1,2-diacyl-sn-glycerol (CDP-DG). Glycerol-3-phosphate ...
Catalyzes the synthesis of cardiolipin (CL) (diphosphatidylglycerol) by specifically transferring a phosphatidyl group from CDP-diacylglycerol to phosphatidylglycerol (PG). CL is a key phospholipid in mitochondrial membranes and plays important roles in maintaining the functional integrity and dynamics of mitochondria under both optimal and stress conditions ...
Accepted name: phosphatidylcholine synthase. Reaction: CDP-diacylglycerol + choline = CMP + phosphatidylcholine. Other name(s): CDP-diglyceride-choline O-phosphatidyltransferase. Systematic name: CDP-diacylglycerol:choline O-phosphatidyltransferase. Comments: Requires divalent cations, with Mn2+ being more effective than Mg2+.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 243666-86-6. References: 1. de Rudder, K.E.E., Sohlenkamp, C. and Geiger, O. Plant-exudated choline is used for rhizobial membrane lipid biosynthesis by phosphatidylcholine synthase. J. Biol. Chem. 274 (1999) 20011-20016. [PMID: 10391951]. 2. Sohlenkamp, C., de Rudder, K.E.E., Röhrs, V., López-Lara, I.M. and Geiger, O. Cloning and characterization of the gene for phosphatidylcholine synthase. J. Biol. Chem. 275 (2000) 18919-18925. [PMID: 10858449]. ...

CDP-diacylglycerol-inositol 3-phosphatidyltransferase - WikipediaCDP-diacylglycerol-inositol 3-phosphatidyltransferase - Wikipedia

CDP-DG:inositol transferase, cytidine diphosphodiglyceride-inositol phosphatidyltransferase, CDP-diacylglycerol:myo-inositol-3- ... CDP-diacylglycerol-inositol phosphatidyltransferase, CDP-diglyceride:inositol transferase, cytidine 5-diphospho-1,2-diacyl-sn- ... phosphatidyltransferase, CDP-diglyceride-inositol transferase, cytidine diphosphoglyceride-inositol phosphatidyltransferase, ... phosphatidyl-1D-myo-inositol Thus, the two substrates of this enzyme are CDP-diacylglycerol and myo-inositol, whereas its two ...
more infohttps://en.wikipedia.org/wiki/CDP-diacylglycerol%E2%80%94inositol_3-phosphatidyltransferase

CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase
      - CDP Diacylglycerol Inositol 3 Phosphatidyltransferase
    ...CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase - CDP Diacylglycerol Inositol 3 Phosphatidyltransferase ...

An enzyme that catalyzes the formation of PHOSPHATIDYLINOSITOL and CMP from CDP-DIACYLGLYCEROL and MYOINOSITOL. ... CDP-DG Inositol Transferase; CDP-Diacylglycerol-Myo-Inositol 3-Phosphatidyltransferase; CDP-Diglyceride-Inositol Transferase; ... CDP DG Inositol Transferase; CDP Diacylglycerol Myo Inositol 3 Phosphatidyltransferase; CDP Diglyceride Inositol Transferase; ... 2 Diacyl sn Glycerol Inositol Transferase; Phosphatidyltransferase, CDPdiglyceride-Inositol; Phosphatidyltransferase, Inositol ...
more infohttp://www.curehunter.com/public/keywordSummaryD051607-CDP-Diacylglycerol-Inositol-3-Phosphatidyltransferase-CDP-Diacylglycerol-Inositol-3-Phosphatidyltransferase.do

CDIPT (CDP-diacylglycerol--inositol 3-phosphatidyltransferase)CDIPT (CDP-diacylglycerol--inositol 3-phosphatidyltransferase)

... inositol 3-phosphatidyltransferase), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol. ... Golgi membrane CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity CDP-diacylglycerol-inositol 3- ... Golgi membrane CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity CDP-diacylglycerol-inositol 3- ... component of membrane diacylglycerol binding manganese ion binding carbohydrate binding alcohol binding CDP-diacylglycerol ...
more infohttp://atlasgeneticsoncology.org/Genes/GC_CDIPT.html

cdipt, CDP-diacylglycerol--inositol 3-phosphatidyltransferase - Creative Biogenecdipt, CDP-diacylglycerol--inositol 3-phosphatidyltransferase - Creative Biogene

CDIPT; CDP-diacylglycerol--inositol 3-phosphatidyltransferase; CDP diacylglycerol inositol 3 phosphatidyltransferase ( ... CDP diacylglycerol inositol 3 phosphatidyltransferase; PtdIns synthase; PI synthase; CDP-diacylglycerol--inositol 3- ... phosphatidylinositol synthase); phosphatidylinositol synthase; PIS; PIS1; MGC1328; CDP diacylglycerol inositol 3 ... Two enzymes, CDP-diacylglycerol synthase and phosphatidylinositol synthase, are involved in the biosynthesis of ...
more infohttps://www.creative-biogene.com/symbolsearch_cdipt.html

CDIPT, ID (CDIPT, PIS, PIS1, CDP-diacylglycerol--inositol 3-phosphatidyltransferase, Phosphatidylinositol Synthase) size: 200ul...CDIPT, ID (CDIPT, PIS, PIS1, CDP-diacylglycerol--inositol 3-phosphatidyltransferase, Phosphatidylinositol Synthase) size: 200ul...

... inositol 3-phosphatidyltransferase Phosphatidylinositol Synthase 01022590324 at Gentaur CDIPT, ID (CDIPT, PIS, PIS1, CDP- ... Order CDIPT ID CDIPT PIS PIS1 CDP-diacylglycerol-- ... CDIPT, ID (CDIPT, PIS, PIS1, CDP-diacylglycerol--inositol 3- ... CDP-diacylglycerol--inositol 3-phosphatidyltransferase; CDP-diacylglycerol--inositol 3-phosphatidyltransferase; CDP- ... CDIPT, ID (CDIPT, PIS, PIS1, CDP-diacylglycerol--inositol 3-phosphatidyltransferase, Phosphatidylinositol Synthase) ...
more infohttps://phosphatidylinositol.com/products/7-mbs-monoclonals/2729-cdipt-id-cdipt-pis-pis1-cdp-diacylglycerol-inositol-3-phosphatidyltransferase-phosphatidylinositol-s

Chromosome 16 - WikipediaChromosome 16 - Wikipedia

CDIPT: CDP-diacylglycerol-inositol 3-phosphatidyltransferase. *CFDP1: Craniofacial development protein 1. *CHDS1: Coronary ... ISBN 978-3-318-02253-7.. *^ Sethakulvichai, W.; Manitpornsut, S.; Wiboonrat, M.; Lilakiatsakun, W.; Assawamakin, A.; Tongsima, ... 3 (2): 243-54. PMID 10464676.. *. Martin J, et al. (2004). "The sequence and analysis of duplication-rich human chromosome 16 ... Chromosome 16 spans about 90 million base pairs (the building material of DNA) and represents just under 3% of the total DNA in ...
more infohttps://en.wikipedia.org/wiki/Chromosome_16

Transcriptional development of phospholipid and lipoprotein metabolism in different intestinal regions of Atlantic salmon (...Transcriptional development of phospholipid and lipoprotein metabolism in different intestinal regions of Atlantic salmon (...

cdp-diacylglycerol-inositol 3-phosphatidyltransferase. CDP. Cytidine diphosphate. CDP-Cho. CDP-choline ... CDP-diacylglycerol:serine phosphatidyltransferase. PtdCho. Phosphatidylcholine. PtdEtn. Phosphatidylethanolamine. PtdGro. ... or CDP-DAG synthetase (cds). DAG is utilized with CDP-choline (CDP-Cho) and CDP-ethanolamine (CDP-Etn) for synthesizing of ... which can be transferred into diacylglycerol (DAG) or CDP-diacylglycerol (CDP-DAG) by phosphatidate phosphatase (plpp and lpin ...
more infohttps://link.springer.com/article/10.1186/s12864-018-4651-8

Chromosome 16 - WikipediaChromosome 16 - Wikipedia

CDIPT: CDP-diacylglycerol-inositol 3-phosphatidyltransferase. *CFDP1: Craniofacial development protein 1. *CHDS1: Coronary ... 978-3-318-02253-7. .. *^ Sethakulvichai, W.; Manitpornsut, S.; Wiboonrat, M.; Lilakiatsakun, W.; Assawamakin, A.; Tongsima, S ... 3 (2): 243-54. doi:10.1089/gte.1999.3.243. PMID 10464676.. *. Martin J, et al. (2004). "The sequence and analysis of ... Chromosome 16 spans about 90 million base pairs (the building material of DNA) and represents just under 3% of the total DNA in ...
more infohttps://en.m.wikipedia.org/wiki/Chromosome_16_

Springer Handbook of Enzymes | Springer for Research & DevelopmentSpringer Handbook of Enzymes | Springer for Research & Development

CDP-diacylglycerol-inositol 3-phosphatidyltransferase Pages 80-92 * CDP-glycerol glycerophosphotransferase Pages 93-95 ... CDP-diacylglycerol-serine O-phosphatidyltransferase Pages 64-75 * Phosphomannan mannosephosphotransferase Pages 76-77 ...
more infohttps://rd.springer.com/book/10.1007/978-3-540-71524-5

GO Gene ListGO Gene List

CDP-diacylglycerol--inositol 3-phosphatidyltransferase. NM_006319. Gene Info. CDK5R2. Cyclin-dependent kinase 5, regulatory ... Diacylglycerol O-acyltransferase 1. NM_012079. Gene Info. DGKA. Diacylglycerol kinase, alpha 80kDa. NM_201444. NM_201445. NM_ ... Diacylglycerol kinase, delta 130kDa. NM_152879. NM_003648. Gene Info. DGKH. Diacylglycerol kinase, eta. NM_178009. NM_001204504 ... Fermitin family member 3. NM_178443. NM_031471. Gene Info. FES. Feline sarcoma oncogene. NM_002005. NM_001143783. NM_001143784 ...
more infohttps://cgap.nci.nih.gov/Genes/GoGeneQuery?PAGE=1&ORG=Hs&GOID=0008289

CDIPT - PCR Primer Pair - SYBR | PrimePCR | Bio-RadCDIPT - PCR Primer Pair - SYBR | PrimePCR | Bio-Rad

CDP-diacylglycerol--inositol 3-phosphatidyltransferase. Aliases:. Not Available. RefSeq:. XM_001105279. Ensembl:. ...
more infohttp://www.bio-rad.com/en-us/prime-pcr-assays/assay/qmcccid0004116-primepcr-sybr-green-assay-cdipt-rhesus-monkey

Human Metabolome Database: Showing metabocard for DG(14:0/18:1(9Z)/0:0) (HMDB0007015)Human Metabolome Database: Showing metabocard for DG(14:0/18:1(9Z)/0:0) (HMDB0007015)

CDP-diacylglycerol--inositol 3-phosphatidyltransferase. General function:. Involved in phosphotransferase activity, for other ... Provides CDP-diacylglycerol an important precursor for the synthesis of phosphatidylinositol, phosphatidylglycerol, and ... Provides CDP-diacylglycerol an important precursor for the synthesis of phosphatidylinositol (PtdIns), phosphatidylglycerol, ... The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by ...
more infohttp://www.hmdb.ca/metabolites/HMDB07015

Human Metabolome Database: Showing metabocard for DG(16:0/16:1(9Z)/0:0) (HMDB0007099)Human Metabolome Database: Showing metabocard for DG(16:0/16:1(9Z)/0:0) (HMDB0007099)

CDP-diacylglycerol--inositol 3-phosphatidyltransferase. General function:. Involved in phosphotransferase activity, for other ... Provides CDP-diacylglycerol an important precursor for the synthesis of phosphatidylinositol, phosphatidylglycerol, and ... Provides CDP-diacylglycerol an important precursor for the synthesis of phosphatidylinositol (PtdIns), phosphatidylglycerol, ... The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by ...
more infohttp://www.hmdb.ca/metabolites/HMDB07099

KEGG PATHWAY: hsa00564KEGG PATHWAY: hsa00564

CDIPT; CDP-diacylglycerol--inositol 3-phosphatidyltransferase [KO:K00999] [EC:2.7.8.11]. 79143 ... ADPRM; ADP-ribose/CDP-alcohol diphosphatase, manganese dependent [KO:K01517] [EC:3.6.1.53 3.6.1.16 3.6.1.13] ... AGPAT3; 1-acylglycerol-3-phosphate O-acyltransferase 3 [KO:K13523] [EC:2.3.1.- 2.3.1.51] ... AGPAT4; 1-acylglycerol-3-phosphate O-acyltransferase 4 [KO:K13523] [EC:2.3.1.- 2.3.1.51] ...
more infohttp://www.genome.jp/dbget-bin/www_bget?pathway+hsa00564

CDIPT - WikipediaCDIPT - Wikipedia

CDP-diacylglycerol-inositol 3-phosphatidyltransferase is an enzyme that in humans is encoded by the CDIPT gene. ... Phosphatidylinositol synthase, a member of the CDP-alcohol phosphatidyl transferase class-I family, is an integral membrane ... "Entrez Gene: CDIPT CDP-diacylglycerol--inositol 3-phosphatidyltransferase (phosphatidylinositol synthase)". Human CDIPT genome ... Two enzymes, CDP-diacylglycerol synthase and phosphatidylinositol synthase, are involved in the biosynthesis of ...
more infohttps://en.wikipedia.org/wiki/CDIPT

Single Gene/enzyme deletions predicted to cause impairment of metabolic networksSingle Gene/enzyme deletions predicted to cause impairment of metabolic networks

CDP-diacylglycerol-inositol 3-phosphatidyltransferase. 2.7.8.11. No evidence. PFF1215w. Sphingomyelin synthase. 2.7.8.27. ... 3-oxoacyl-acyl-carrier protein synthase I/II (FABB/F). 2.3.1.41. ... E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase. 1.17.7.1 ...
more infohttp://mpmp.huji.ac.il/maps/enz_imp.html

A revised biosynthetic pathway for phosphatidylinositol in Mycobacteria<...A revised biosynthetic pathway for phosphatidylinositol in Mycobacteria<...

When CDP-diacylglycerol and [ 14C]1L-myo-inositol 1-phosphate were incubated with the cell wall components of Mycobacterium ... When CDP-diacylglycerol and [ 14C]1L-myo-inositol 1-phosphate were incubated with the cell wall components of Mycobacterium ... When CDP-diacylglycerol and [ 14C]1L-myo-inositol 1-phosphate were incubated with the cell wall components of Mycobacterium ... When CDP-diacylglycerol and [ 14C]1L-myo-inositol 1-phosphate were incubated with the cell wall components of Mycobacterium ...
more infohttps://kyushu-u.pure.elsevier.com/en/publications/a-revised-biosynthetic-pathway-for-phosphatidylinositol-in-mycoba

Search Articles | University of Toronto LibrariesSearch Articles | University of Toronto Libraries

cdp-diacylglycerol-inositol 3-phosphatidyltransferase (66) 66 Filter by. Remove filter. saccharomyces-cerevisiae (64) 64 ... RAT-LIVER , SIGNAL-TRANSDUCTION , DIACYLGLYCEROL , MEMBRANE , BIOCHEMISTRY & MOLECULAR BIOLOGY , SERINE PALMITOYLTRANSFERASE , ... Full Text SMS overexpression and knockdown: Impact on cellular sphingomyelin and diacylglycerol metabolism, and cell apoptosis ... 3. Full Text Lipidomics identifies cardiolipin oxidation as a mitochondrial target for redox therapy of brain injury ...
more infohttps://query.library.utoronto.ca/index.php/search/q?kw=SubjectTerms:Transferases%20

Noca 3239 protein (Nocardioides sp. JS614) - STRING interaction networkNoca 3239 protein (Nocardioides sp. JS614) - STRING interaction network

CDP-diacylglycerol-phosphatidylglycerol phosphatidyltransferase. CDP-diacylglycerol inositol 3-phosphatidyltransferase. 0.919. ... CDP-diacylglycerol-phosphatidylglycerol phosphatidyltransferase. CDP-diacylglycerol--glycerol-3-phosphate 3- ... CDP-diacylglycerol inositol 3-phosphatidyltransferase. recA protein; Can catalyze the hydrolysis of ATP in the presence of ... CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase. recA protein; Can catalyze the hydrolysis of ATP in the ...
more infohttps://string-db.org/network/196162.Noca_3239

Bra004101 protein (Brassica rapa) - STRING interaction networkBra004101 protein (Brassica rapa) - STRING interaction network

CDP-alcohol phosphatidyltransferase/ CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase (254 aa) ... AT4G38570 (E=1e-113) PIS2 , PIS2 (PROBABLE CDP-DIACYLGLYCEROL--INOSITOL 3-PHOSPHATIDYLTRANSFERASE 2); phosphotransferase, for ... CDP-alcohol phosphatidyltransferase/ CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase ... CDP-alcohol phosphatidyltransferase/ CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase ...
more infohttps://string-db.org/network/3711.Bra004101.1-P

IMP: Integrative Multi-species PredictionIMP: Integrative Multi-species Prediction

CDP-diacylglycerol--inositol 3-phosphatidyltransferase (phosphatidylinositol synthase). 0.017. cd40lg. CD40 ligand. 0.016. ...
more infohttp://imp.princeton.edu/predictions/process_ortho/fly-context-global/14352/?gene=139414

Code System ConceptCode System Concept

CDP-diglyceride-inositol phosphatidyltransferase Current Synonym true false 43865012 Phosphatidylinositol synthase Current ... Cytosine diphosphate (CDP) diacylglycerol-inositol 3-phosphatidyltransferase Current Synonym true false 2914041012 Cytosine ... Cytosine diphosphate diacylglycerol-inositol 3-phosphatidyltransferase (substance). Code System Preferred Concept Name. ... diphosphate diacylglycerol-inositol 3-phosphatidyltransferase Current Synonym true false 43863017 CDPdiacylglycerol-inositol 3- ...
more infohttps://phinvads.cdc.gov/vads/ViewCodeSystemConcept.action?oid=2.16.840.1.113883.6.96&code=26181000

IMP: Integrative Multi-species PredictionIMP: Integrative Multi-species Prediction

CDP-diacylglycerol--inositol 3-phosphatidyltransferase. 0.011. SLC25A44. solute carrier family 25, member 44. 0.011. ... signal transducer and activator of transcription 3 (acute-phase response factor). 0.017. ...
more infohttp://imp.princeton.edu/predictions/process/human-context-global/2169/?gene=8894

Gene expression profile of AIDS-related Kaposis sarcoma | BMC Cancer | Full TextGene expression profile of AIDS-related Kaposi's sarcoma | BMC Cancer | Full Text

Only 2,6%-3,0% of the tags occurred five times or more, 3518, 3444 and 2908 were seen between two and four times, and 11,326, ... Table 3 HHV8 tag counts in the AIDS-KS SAGE libraries For comparison tag numbers for each group were normalized to 50,000. ... Figure 3 Semi-quantitative RT-PCR analysis of differentially expressed genes PCR-analysis was performed for six genes on cDNA ... In 1994, Chang et al. [3] identified two fragments of herpesvirus-like DNA in the lesions of a patient with AIDS-KS. Since this ...
more infohttps://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-3-7

Glycerophospholipid Biosynthetic Pathway (Homo sapiens) - WikiPathwaysGlycerophospholipid Biosynthetic Pathway (Homo sapiens) - WikiPathways

CDP-DAG. CMP. CDP-diacylglyreol-inositol 3-phosphatidyltransferase. EC 2.7.8.11. 5, 23, 110, 120, 164. 1-phosphatidylinositol 4 ... CDP-choline. Metabolite. 49086 (ChEBI) CDP-choline:1-alkyl-2-acyl-sn-glycerophosphocholine transferase (Diacylglycerol ... Inositol. Metabolite. 892 (PubChem-compound) L-serine-phosphatidylethanolamine phosphatidyltransferase EC 2.7.8.29. Protein. ... CDP-ethanolamine). 6, 46, 49, 68, 259. CMP. ATP. ADP. CTP. PPi. Ethanolamine. Diacylglycerol O-acyltransferase. EC 2.3.1.20. 75 ...
more infohttps://www.wikipathways.org/index.php?title=Pathway:WP2533&
  • Glycerol-3-phosphate is first acylated with acyl-coenzyme A (acyl-CoA) to form lysophosphatidic acid, which is then acylated with another molecule of acyl-CoA to yield phosphatidic acid. (hmdb.ca)
  • Since diacylglycerols are synthesized via phosphatidic acid, they will usually contain a saturated fatty acid at the C-1 position on the glycerol moiety and an unsaturated fatty acid at the C-2 position. (hmdb.ca)
  • Studies showed that supplementation with n-3 PUFA can alter gene expressions toward a more anti-atherogenic and anti-inflammatory profile (Bouwens et al. (biomedcentral.com)
  • Chromosome 16 spans about 90 million base pairs (the building material of DNA) and represents just under 3% of the total DNA in cells . (wikipedia.org)
  • Synthesis of diacylglycerol begins with glycerol-3-phosphate, which is derived primarily from dihydroxyacetone phosphate, a product of glycolysis (usually in the cytoplasm of liver or adipose tissue cells). (hmdb.ca)
  • Transcriptomic profiles after n-3 PUFA supplementation indicate changes in glycerophospholipid metabolism in both subgroups and sphingolipid metabolism in non-responders. (biomedcentral.com)
  • In sum, results highlight key differences in lipid metabolism of non-responders compared to responders after an n-3 PUFA supplementation, which may explain the inter-individual variability in plasma TG response. (biomedcentral.com)
  • A very low, but significant level of free [ 3 H]inositol was incorporated into PI in mycobacterial cell wall preparations, but not in recombinant E. coli cell homogenates. (elsevier.com)
  • Six subjects did not lower their plasma TG (+9 %) levels (non-responders) and were matched to 6 subjects who lowered TG (−41 %) concentrations (responders) after the n-3 PUFA supplementation. (biomedcentral.com)
  • Studies have demonstrated large within-population heterogeneity in plasma triacylglycerol (TG) response to n-3 PUFA supplementation. (biomedcentral.com)