Cdh1 is an activator of the anaphase-promoting complex-cyclosome, and is involved in substrate recognition. It associates with the complex in late MITOSIS from anaphase through G1 to regulate activity of CYCLIN-DEPENDENT KINASES and to prevent premature DNA replication.
Protrusion of abdominal structures into the THORAX as a result of congenital or traumatic defects in the respiratory DIAPHRAGM.
An E3 ubiquitin ligase primarily involved in regulation of the metaphase-to-anaphase transition during MITOSIS through ubiquitination of specific CELL CYCLE PROTEINS. Enzyme activity is tightly regulated through subunits and cofactors, which modulate activation, inhibition, and substrate specificity. The anaphase-promoting complex, or APC-C, is also involved in tissue differentiation in the PLACENTA, CRYSTALLINE LENS, and SKELETAL MUSCLE, and in regulation of postmitotic NEURONAL PLASTICITY and excitability.
Complexes of enzymes that catalyze the covalent attachment of UBIQUITIN to other proteins by forming a peptide bond between the C-terminal GLYCINE of UBIQUITIN and the alpha-amino groups of LYSINE residues in the protein. The complexes play an important role in mediating the selective-degradation of short-lived and abnormal proteins. The complex of enzymes can be broken down into three components that involve activation of ubiquitin (UBIQUITIN-ACTIVATING ENZYMES), conjugation of ubiquitin to the ligase complex (UBIQUITIN-CONJUGATING ENZYMES), and ligation of ubiquitin to the substrate protein (UBIQUITIN-PROTEIN LIGASES).
Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body.
Highly conserved proteins that specifically bind to and activate the anaphase-promoting complex-cyclosome, promoting ubiquitination and proteolysis of cell-cycle-regulatory proteins. Cdc20 is essential for anaphase-promoting complex activity, initiation of anaphase, and cyclin proteolysis during mitosis.
Ethers that are linked to a benzene ring structure.
Reversibly catalyze the oxidation of a hydroxyl group of carbohydrates to form a keto sugar, aldehyde or lactone. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.; EC 1.1.2.; and 1.1.99.
A subunit of the anaphase-promoting complex whose primary function is to provide structural support for the catalytic and substrate-recognition modules of the complex. Apc5, along with Apc4, tethers the tetratricopeptide-coactivator binding subcomplex to the main structural subunit, Apc1.
Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.
A subunit of the anaphase-promoting complex whose primary function is to provide structural support for the catalytic and substrate-recognition modules of the complex. Apc4, along with Apc5, tethers the tetratricopeptide-coactivator binding subcomplex to the main structural subunit, Apc1.
Addition of methyl groups to DNA. DNA methyltransferases (DNA methylases) perform this reaction using S-ADENOSYLMETHIONINE as the methyl group donor.
An enzyme that catalyzes the oxidation of 1-pyrroline-5-carboxylate to L-GLUTAMATE in the presence of NAD. Defects in the enzyme are the cause of hyperprolinemia II.
The largest subunit of the anaphase-promoting complex. It acts primarily as a scaffold for the proper organization and arrangement of subunits. The C-terminal region of Apc1 contains a series of tandem amino acid repeats that are also seen in the 26S proteasome regulatory particle, and may assist with forming and stabilizing protein-protein interactions.
Securin is involved in the control of the metaphase-anaphase transition during MITOSIS. It promotes the onset of anaphase by blocking SEPARASE function and preventing proteolysis of cohesin and separation of sister CHROMATIDS. Overexpression of securin is associated with NEOPLASTIC CELL TRANSFORMATION and tumor formation.
A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.
A cyclin subtype that is transported into the CELL NUCLEUS at the end of the G2 PHASE. It stimulates the G2/M phase transition by activating CDC2 PROTEIN KINASE.
Tumors or cancer of the STOMACH.
A class of enzymes that catalyze the formation of a bond between two substrate molecules, coupled with the hydrolysis of a pyrophosphate bond in ATP or a similar energy donor. (Dorland, 28th ed) EC 6.
A highly evolutionarily conserved subunit of the anaphase-promoting complex (APC-C) containing multiple 34-amino-acid tetratricopeptide repeats. These domains, also found in Apc subunits 6, 7, and 8, have been shown to mediate protein-protein interactions, suggesting that Apc3 may assist in coordinating the juxtaposition of the catalytic and substrate recognition module subunits relative to co-activators and APC-C inhibitors.
A family of structurally-related proteins that were originally identified by their ability to complex with cyclin proteins (CYCLINS). They share a common domain that binds specifically to F-BOX MOTIFS. They take part in SKP CULLIN F-BOX PROTEIN LIGASES, where they can bind to a variety of F-BOX PROTEINS.
Various conditions with the symptom of HEADACHE. Headache disorders are classified into major groups, such as PRIMARY HEADACHE DISORDERS (based on characteristics of their headache symptoms) and SECONDARY HEADACHE DISORDERS (based on their etiologies). (International Classification of Headache Disorders, 2nd ed. Cephalalgia 2004: suppl 1)
The act of ligating UBIQUITINS to PROTEINS to form ubiquitin-protein ligase complexes to label proteins for transport to the PROTEASOME ENDOPEPTIDASE COMPLEX where proteolysis occurs.
The phase of cell nucleus division following PROPHASE, when the breakdown of the NUCLEAR ENVELOPE occurs and the MITOTIC SPINDLE APPARATUS enters the nuclear region and attaches to the KINETOCHORES.
The phase of cell nucleus division following METAPHASE, in which the CHROMATIDS separate and migrate to opposite poles of the spindle.
Together with the Apc11 subunit, forms the catalytic core of the E3 ubiquitin ligase anaphase-promoting complex (APC-C). Its N-terminus has cullin domains which associate with the RING FINGER DOMAINS of Apc11. Apc2 also interacts with the E2 ubiquitin ligases involved in APC-C ubiquitination reactions.
Together with the Apc2 subunit, forms the catalytic core of the E3 ubiquitin ligase, anaphase-promoting complex-cyclosome. It has a RING H2 domain which interacts with the cullin domain of Apc2. Apc11 also interacts with the E2 ubiquitin ligases involved in APC-C ubiquitination reactions.
A diverse class of enzymes that interact with UBIQUITIN-CONJUGATING ENZYMES and ubiquitination-specific protein substrates. Each member of this enzyme group has its own distinct specificity for a substrate and ubiquitin-conjugating enzyme. Ubiquitin-protein ligases exist as both monomeric proteins multiprotein complexes.
Autosomal recessive hereditary disorders characterized by congenital SENSORINEURAL HEARING LOSS and RETINITIS PIGMENTOSA. Genetically and symptomatically heterogeneous, clinical classes include type I, type II, and type III. Their severity, age of onset of retinitis pigmentosa and the degree of vestibular dysfunction are variable.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Apc10 is necessary for coactivator-dependent substrate recognition by the anaphase-promoting complex-cyclosome. It binds the Apc2 subunit, which is a part of the catalytic core, and interacts with coactivators Cdh1 or Cdc20 to recruit substrates to the complex.
Mechanosensing organelles of hair cells which respond to fluid motion or fluid pressure changes. They have various functions in many different animals, but are primarily used in hearing.
Any detectable and heritable alteration in the lineage of germ cells. Mutations in these cells (i.e., "generative" cells ancestral to the gametes) are transmitted to progeny while those in somatic cells are not.
The period of the CELL CYCLE preceding DNA REPLICATION in S PHASE. Subphases of G1 include "competence" (to respond to growth factors), G1a (entry into G1), G1b (progression), and G1c (assembly). Progression through the G1 subphases is effected by limiting growth factors, nutrients, or inhibitors.
Sensory cells in the organ of Corti, characterized by their apical stereocilia (hair-like projections). The inner and outer hair cells, as defined by their proximity to the core of spongy bone (the modiolus), change morphologically along the COCHLEA. Towards the cochlear apex, the length of hair cell bodies and their apical STEREOCILIA increase, allowing differential responses to various frequencies of sound.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
A disaccharide consisting of two glucose units in beta (1-4) glycosidic linkage. Obtained from the partial hydrolysis of cellulose.
Presence of less than the normal amount of hair. (Dorland, 27th ed)
Gradual bilateral hearing loss associated with aging that is due to progressive degeneration of cochlear structures and central auditory pathways. Hearing loss usually begins with the high frequencies then progresses to sounds of middle and low frequencies.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
Pathophysiological conditions of the FETUS in the UTERUS. Some fetal diseases may be treated with FETAL THERAPIES.
The musculofibrous partition that separates the THORACIC CAVITY from the ABDOMINAL CAVITY. Contraction of the diaphragm increases the volume of the thoracic cavity aiding INHALATION.
Substances used to destroy or inhibit the action of rats, mice, or other rodents.
Adherence of cells to surfaces or to other cells.
The reduction or regulation of the population of noxious, destructive, or dangerous rodents through chemical, biological, or other means.
Diseases of rodents of the order RODENTIA. This term includes diseases of Sciuridae (squirrels), Geomyidae (gophers), Heteromyidae (pouched mice), Castoridae (beavers), Cricetidae (rats and mice), Muridae (Old World rats and mice), Erethizontidae (porcupines), and Caviidae (guinea pigs).
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
Hair-like extensions on specialized epidermal surfaces of plants which protect against damage from insects, animals, light degradation and fungal infection. Trichomes may also occur on certain unicellular EUKARYOTES.
Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.
A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.
A type of nuclear polyploidization in which multiple cycles of DNA REPLICATION occur in the absence of CELL DIVISION and result in a POLYPLOID CELL.
A thin layer of cells forming the outer integument of seed plants and ferns. (Random House Unabridged Dictionary, 2d ed)

Inhibitory phosphorylation of the APC regulator Hct1 is controlled by the kinase Cdc28 and the phosphatase Cdc14. (1/136)

BACKGROUND: Exit from mitosis requires inactivation of mitotic cyclin-dependent kinases (CDKs). A key mechanism of CDK inactivation is ubiquitin-mediated cyclin proteolysis, which is triggered by the late mitotic activation of a ubiquitin ligase known as the anaphase-promoting complex (APC). Activation of the APC requires its association with substoichiometric activating subunits termed Cdc20 and Hct1 (also known as Cdh1). Here, we explore the molecular function and regulation of the APC regulatory subunit Hct1 in Saccharomyces cerevisiae. RESULTS: Recombinant Hct1 activated the cyclin-ubiquitin ligase activity of APC isolated from multiple cell cycle stages. APC isolated from cells arrested in G1, or in late mitosis due to the cdc14-1 mutation, was more responsive to Hct1 than APC isolated from other stages. We found that Hct1 was phosphorylated in vivo at multiple CDK consensus sites during cell cycle stages when activity of the cyclin-dependent kinase Cdc28 is high and APC activity is low. Purified Hct1 was phosphorylated in vitro at these sites by purified Cdc28-cyclin complexes, and phosphorylation abolished the ability of Hct1 to activate the APC in vitro. The phosphatase Cdc14, which is known to be required for APC activation in vivo, was able to reverse the effects of Cdc28 by catalyzing Hct1 dephosphorylation and activation. CONCLUSIONS: We conclude that Hct1 phosphorylation is a key regulatory mechanism in the control of cyclin destruction. Phosphorylation of Hct1 provides a mechanism by which Cdc28 blocks its own inactivation during S phase and early mitosis. Following anaphase, dephosphorylation of Hct1 by Cdc14 may help initiate cyclin destruction.  (+info)

Pds1 and Esp1 control both anaphase and mitotic exit in normal cells and after DNA damage. (2/136)

The separation of sister chromatids in anaphase is followed by spindle disassembly and cytokinesis. These events are governed by the anaphase-promoting complex (APC), which triggers the ubiquitin-dependent proteolysis of key regulatory proteins: anaphase requires the destruction of the anaphase inhibitor Pds1, whereas mitotic exit requires the destruction of mitotic cyclins and the inactivation of Cdk1. We find that Pds1 is not only an inhibitor of anaphase, but also blocks cyclin destruction and mitotic exit by a mechanism independent of its effects on sister chromatid separation. Pds1 is also required for the mitotic arrest and inhibition of cyclin destruction that occurs after DNA damage. Even in anaphase cells, where Pds1 levels are normally low, DNA damage stabilizes Pds1 and prevents cyclin destruction and mitotic exit. Pds1 blocks cyclin destruction by inhibiting its binding partner Esp1. Mutations in ESP1 delay cyclin destruction; overexpression of ESP1 causes premature cyclin destruction in cells arrested in metaphase by spindle defects and in cells arrested in metaphase and anaphase by DNA damage. The effects of Esp1 are dependent on Cdc20 (an activating subunit of the APC) and on several additional proteins (Cdc5, Cdc14, Cdc15, Tem1) that form a regulatory network governing mitotic exit. We speculate that the inhibition of cyclin destruction by Pds1 may contribute to the ordering of late mitotic events by ensuring that mitotic exit is delayed until after anaphase is initiated. In addition, the stabilization of Pds1 after DNA damage provides a mechanism to delay both anaphase and mitotic exit while DNA repair occurs.  (+info)

Regulation of APC activity by phosphorylation and regulatory factors. (3/136)

Ubiquitin-dependent proteolysis of Cut2/Pds1 and Cyclin B is required for sister chromatid separation and exit from mitosis, respectively. Anaphase-promoting complex/cyclosome (APC) specifically ubiquitinates Cut2/Pds1 at metaphase-anaphase transition, and ubiquitinates Cyclin B in late mitosis and G1 phase. However, the exact regulatory mechanism of substrate-specific activation of mammalian APC with the right timing remains to be elucidated. We found that not only the binding of the activators Cdc20 and Cdh1 and the inhibitor Mad2 to APC, but also the phosphorylation of Cdc20 and Cdh1 by Cdc2-Cyclin B and that of APC by Polo-like kinase and cAMP-dependent protein kinase, regulate APC activity. The cooperation of the phosphorylation/dephosphorylation and the regulatory factors in regulation of APC activity may thus control the precise progression of mitosis.  (+info)

Expression of the CDH1-associated form of the anaphase-promoting complex in postmitotic neurons. (4/136)

The anaphase-promoting complex/cyclosome (APC) is a tightly cell cycle-regulated ubiquitin-protein ligase that targets cyclin B and other destruction box-containing proteins for proteolysis at the end of mitosis and in G1. Recent work has shown that activation of the APC in mitosis depends on CDC20, whereas APC is maintained active in G1 via association with the CDC20-related protein CDH1. Here we show that the mitotic activator CDC20 is the only component of the APC ubiquitination pathway whose expression is restricted to proliferating cells, whereas the APC and CDH1 are also expressed in several mammalian tissues that predominantly contain differentiated cells, such as adult brain. Immunocytochemical analyses of cultured rat hippocampal neurons and of mouse and human brain sections indicate that the APC and CDH1 are ubiquitously expressed in the nuclei of postmitotic terminally differentiated neurons. The APC purified from brain contains all core subunits known from proliferating cells and is tightly associated with CDH1. Purified brain APC(CDH1) has a high cyclin B ubiquitination activity that depends less on the destruction box than on the activity of mitotic APC(CDC20). On the basis of these results, we propose that the functions of APC(CDH1) are not restricted to controlling cell-cycle progression but may include the ubiquitination of yet unidentified substrates in differentiated cells.  (+info)

The mammalian Fizzy and Fizzy-related genes are regulated at the transcriptional and post-transcriptional levels. (5/136)

The cyclosome pathway of ubiquitin-mediated proteolysis plays an essential role in cell cycle control. The multisubunit cyclosome is regulated by transient interactions with Fizzy (Fzy) and Fizzy-related (Fzr) genes. We report here that both Fzy and Fzr are transcribed in a cell cycle specific but distinct manner. Fzy transcription starts after the restriction point in late G1 and ceases upon cell division. Fzr transcription also ceases upon cell division but resumes already in mid G1, before the restriction point, and takes place also in G0. Fzr has further a striking cell cycle specific pattern of mRNA stability. During most of the cell cycle its message is fairly stable, however upon exit from mitosis it is rapidly degraded. This result is puzzling because Fzr is essential for cyclosome activity in G1, and points to a complex pattern of Fzr regulation.  (+info)

Two different modes of cyclin clb2 proteolysis during mitosis in Saccharomyces cerevisiae. (6/136)

Sister chromatid separation and mitotic exit are triggered by the anaphase-promoting complex (APC/C) which is a multi-subunit ubiquitin ligase required for proteolytic degradation of various target proteins. Cdc20 and Cdh1 are substrate-specific activators of the APC/C. It was previously proposed that Cdh1 is essential for proteolysis of the yeast mitotic cyclin Clb2. We show that Clb2 proteolysis is triggered by two different modes during mitosis. A fraction of Clb2 is degraded during anaphase in the absence of Cdh1. However, a second fraction of Clb2 remains stable during anaphase and is degraded in a Cdh1-dependent manner as cells exit from mitosis. Most of cyclin Clb3 is degraded independently of Cdh1. Our data imply that degradation of mitotic cyclins is initiated by a Cdh1-independent mechanism.  (+info)

The KEN box: an APC recognition signal distinct from the D box targeted by Cdh1. (7/136)

The ordered progression through the cell cycle depends on regulating the abundance of several proteins through ubiquitin-mediated proteolysis. Degradation is precisely timed and specific. One key component of the degradation system, the anaphase promoting complex (APC), is a ubiquitin protein ligase. It is activated both during mitosis and late in mitosis/G(1), by the WD repeat proteins Cdc20 and Cdh1, respectively. These activators target distinct sets of substrates. Cdc20-APC requires a well-defined destruction box (D box), whereas Cdh1-APC confers a different and as yet unidentified specificity. We have determined the sequence specificity for Cdh1-APC using two assays, ubiquitination in a completely defined and purified system and degradation promoted by Cdh1-APC in Xenopus extracts. Cdc20 is itself a Cdh1-APC substrate. Vertebrate Cdc20 lacks a D box and therefore is recognized by Cdh1-APC through a different sequence. By analysis of Cdc20 as a substrate, we have identified a new recognition signal. This signal, composed of K-E-N, serves as a general targeting signal for Cdh1-APC. Like the D box, it is transposable to other proteins. Using the KEN box as a template, we have identified cell cycle genes Nek2 and B99 as additional Cdh1-APC substrates. Mutation in the KEN box stabilizes all three proteins against ubiquitination and degradation.  (+info)

Mitotic regulation of the APC activator proteins CDC20 and CDH1. (8/136)

The ordered activation of the ubiquitin protein ligase anaphase-promoting complex (APC) or cyclosome by CDC20 in metaphase and by CDH1 in telophase is essential for anaphase and for exit from mitosis, respectively. Here, we show that CDC20 can only bind to and activate the mitotically phosphorylated form of the Xenopus and the human APC in vitro. In contrast, the analysis of phosphorylated and nonphosphorylated forms of CDC20 suggests that CDC20 phosphorylation is neither sufficient nor required for APC activation. On the basis of these results and the observation that APC phosphorylation correlates with APC activation in vivo, we propose that mitotic APC phosphorylation is an important mechanism that controls the proper timing of APC(CDC20) activation. We further show that CDH1 is phosphorylated in vivo during S, G2, and M phase and that CDH1 levels fluctuate during the cell cycle. In vitro, phosphorylated CDH1 neither binds to nor activates the APC as efficiently as does nonphosphorylated CDH1. Nonphosphorylatable CDH1 mutants constitutively activate APC in vitro and in vivo, whereas mutants mimicking the phosphorylated form of CDH1 are constitutively inactive. These results suggest that mitotic kinases have antagonistic roles in regulating APC(CDC20) and APC(CDH1); the phosphorylation of APC subunits is required to allow APC activation by CDC20, whereas the phosphorylation of CDH1 prevents activation of the APC by CDH1. These mechanisms can explain the temporal order of APC activation by CDC20 and CDH1 and may help to ensure that exit from mitosis is not initiated before anaphase has occurred.  (+info)

Prophase I arrest, referred to as the germinal vesicle (GV) stage, is a conserved feature of oocytes across species (Whitaker, 1996; Mehlmann, 2005; Jones, 2008). In mice, as in all mammals, GV arrest begins shortly after meiotic recombination in fetal life. Periodic, non-hormonal recruitment of a small number of quiescent follicles into the growing pool after birth leads to follicle growth and eventual ovulation, both of which are hormone dependent. It is only near the time of ovulation in the fully grown oocytes of mature follicles that a rise in luteinizing hormone (LH) breaks this arrest, causing GV breakdown (GVB).. A high level of cAMP, generated from a Gs-coupled oocyte receptor, is needed to maintain arrest in fully grown oocytes (Cho et al., 1974; Magnusson and Hillensjo, 1977; Bornslaeger et al., 1986; Mehlmann et al., 2002; Freudzon et al., 2005). This is enhanced by cGMP from the surrounding cumulus cells, which inhibits phosphodiesterase 3A (PDE3A) activity, preventing cAMP ...
Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated
Figure 1. Cdh1 phosphorylation at Cdk sites promotes Cdh1 stability. A, Schematic of nine conserved sites of potential Cdk phosphorylation in Cdh1 (S/T-P), numbered according to human Cdh1. B, Lysates of 293T cells transfected with GFP-Cdh1 WT, 9A, or 9D were immunoblotted using a polyclonal antibody to Cdh1 or Erk, the latter to serve as a loading control. Quantitation of Cdh1 levels normalized by Erk revealed that the levels of the 9D and 9A mutant proteins were, respectively, increased by 122% and reduced by 57% relative to wild-type Cdh1 in 293T cells (average of 4 experiments). C, Lysates of Neuro2A cells transfected with GFP-Cdh1 WT, 9A, or 9D and treated with MG132 (5 μm) or the vehicle control DMSO were immunoblotted for GFP or Erk. The levels of the 9D and 9A mutant proteins were, respectively, increased by 150% and reduced by 37% relative to wild-type Cdh1 in Neuro2A cells (average of 2 experiments). MG132 treatment, respectively, increased WT, 9A, and 9D levels by 80, 134, and 9%. D, ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
CDH23 - CDH23 (untagged)-Human cadherin-related 23 (CDH23) transcript variant 3 available for purchase from OriGene - Your Gene Company.
Rational: Poor prognosis epithelial-derived cancers often exhibit morphologic and molecular changes characteristic of an epithelial to mesenchymal transition (EMT). EMT markers are predominantly found in tumors with a basal-like phenotype. Increased expression of the mesenchymal cadherins N-cadherin and/or Cadherin-11 (CDH11) and decreased E-cadherin, have been associated with both EMT and tumor progression. Importantly, CDH11 is a therapeutic target in rheumatoid arthritis (RA), an inflammatory disease with properties often compared with cancer. As CDH11 antibody based therapeutics are in clinical trials for RA and we recently showed that the arthritis drug celecoxib has the structural potential to bind CDH11, there is a strong possibility that, if CDH11can be shown to drive malignant progression rather than simply be associated with it, therapeutic options may be rapidly developed.. Results: We show that CDH11 is increased early in breast cancer and ductal carcinoma in-situ. CDH11 knockdown ...
CHO-Anti-Human CDH17 MAb stable cell line is clonally-derived from a CHO cell line, which has been transfected with an Anti-human CDH17 MAb gene to allow expression of the MAb. It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
Monoclonal antibody against Cadherin, E- expressed by Cdh1 for use in FFPE, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot against Drosophila, Xenopus
A non-religious forum for Genesis II Church of Health & Healing set up to serve mankind. We focus on healing and welcome people of all diversities, all beliefs and walks of life; to join us in our mission to bring health to the world.
Complete information for CDH11 gene (Protein Coding), Cadherin 11, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
####### Forum Help ####### Forum Rules I need to contact Jim Humble Contact jimhumble.is/contact The MMS Protocols Youll find them at the...
Hey everyone, I thought it important to share what things to look out for while doing your protocol...very important for speedy recovery. Taken from "" ...
Liver cancer is the fifth most commonly diagnosed and the second most lethal malignancies worldwide, in which hepatocellular carcinoma (HCC) represents the majority subtype. High mortality rate of HCC is due to lack of effective treatments and early detection methods. Activation of cadherin-17 (CDH17)/β-catenin axis is found by our team in HCC and targeting components of this axis associated with anti-tumorigenesis. With limited knowledge on this axis in HCC, I plan to study molecules related to this axis as a way to uncover the cellular mechanism of this axis in liver tumorigenesis. Gene profiling data was re-analyzed to search for CDH17-associated genes in HCC clinical samples. The patient cohort was segregated into CDH17-high and CDH17-low group according to tumor/adjacent non-tumor expression ratio of CDH17. Serine peptidase inhibitor, Kazal type 1 (SPINK1) was found highly expressed in CDH17-high cases and its over-expression accounted for 73 % of total studied cases. Gene manipulation and ...
Overexpression of mitotic cyclin CLB2 results in premature spindle elongation in swe1Δ mutants.A. Overexpression of CLB2 is toxic to swe1Δ mutants. WT and swe
In contrast to the absence of any significant requirement for Clb3 proteolysis for mitotic exit, mitotic Clb3 proteolysis is required for control of Start. Start is conditional on cells attaining a sufficient cell size; it depends on Cln3 as an initial upstream signal, and on the Cln1,2-dependent positive feedback loop (Cross and Tinkelenberg 1991; Dirick and Nasmyth 1991; Cross 1995; Skotheim et al. 2008). Start is also specifically blocked by mating pheromones (Cross 1995). All of these controls are abrogated by removal of the Clb3 D box: CLB3∆db cells pass Start even when very small, as indicated by the absence of nuclear Whi5, and by accelerated budding and DNA replication, in a Cln3-independent fashion; CLB3∆db results in mating factor insensitivity, and eliminates the requirement for any of CLN1,2,3 CLB5,6. Rescue of CLN deficiency by cyclins has been previously reported, but has involved expression of the rescuing cyclins from a strong promoter such as ADH1 (Koff et al. 1991; Léopold ...
Organ morphogenesis in multicellular organisms relies on the coordinated progression of cell proliferation and cell growth. Compared with animals, plants often undergo far more extensive post‐mitotic cell growth, sometimes up to 1000‐fold of original size, and an increase in cell size generally contributes to a larger extent to organ growth. It is also known that some cell types, such as neurons and various hair cells in insects, animals and plants, undergo massive cell growth during differentiation and this is vital for their specialised physiology and function (Sugimoto‐Shirasu and Roberts, 2003). Since the final size of cells is often fairly constant under given conditions, the duration of post‐mitotic cell growth is likely to be developmentally programmed. We still know surprisingly little about how cell size is determined and how developmental signals link to this control. Most of loss‐of‐function mutants with developmental defects display reduced cell‐size phenotypes, ...
The epithelial-to-mesenchymal transition (EMT) transcriptional program is characterized by repression of E-cadherin (CDH1) and induction of N-cadherin (CDH2), and mesenchymal genes like vimentin (VIM). Placenta-specific 8 (PLAC8) has been implicated in colon cancer; however, how PLAC8 contributes to disease is unknown, and endogenous PLAC8 protein has not been studied. We analyzed zebrafish and human tissues and found that endogenous PLAC8 localizes to the apical domain of differentiated intestinal epithelium. Colon cancer cells with elevated PLAC8 levels exhibited EMT features, including increased expression of VIM and zinc finger E-box binding homeobox 1 (ZEB1), aberrant cell motility, and increased invasiveness. In contrast to classical EMT, PLAC8 overexpression reduced cell surface CDH1 and upregulated P-cadherin (CDH3) without affecting CDH2 expression. PLAC8-induced EMT was linked to increased phosphorylated ERK2 (p-ERK2), and ERK2 knockdown restored cell surface CDH1 and suppressed CDH3, ...
Full-length coding sequences of two novel human cadherin cDNAs were obtained by sequence analysis of several EST clones and 5 and 3 rapid amplification of cDNA ends (RACE) products. Exons for a third cDNA sequence were identified in a public-domain
CDH1 - human gene knockout kit via CRISPR, 1 kit. |dl||dt|Kit Component:|/dt||dd|- |strong|KN220731G1|/strong|, CDH1 gRNA vector 1 in |a href=http://www.origene.com/CRISPR-CAS9/Detail.
As a guild, our mission is to raise money for Seattle Childrens Hospital. But we are also a group of parents, friends and family - all touched in some way by CDH. And as such, we seek to raise awareness of this life-threatening birth defect so that we may inspire hope and empowerment for those…
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Complete information for CDH12P1 gene (Pseudogene), Cadherin 12 Pseudogene 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Mouse monoclonal antibody raised against a partial recombinant CDH2. CDH2 (NP_001783, 807 a.a. ~ 906 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. (H00001000-M10) - Products - Abnova
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Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
Summary of CDH13 (CDHH) expression in human tissue. Distinct membranous and cytoplasmic expression in muscle tissues, renal glomeruli, cerebral cortex, in endothelial cells and in basal cells of squamous epithelia.
Expression of CDH3 (CDHP, PCAD) in human tissue. Overview of the antibody staining with HPA001767 and CAB002487 in immunohistochemistry
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CDH1 E273K lies within the Cadherin domain 2 of the Cdh1 protein (UniProt.org). E273K has not been characterized in the scientific literature and therefore, its effect on Cdh1 protein function is unknown (PubMed, May 2020 ...
Although interferon-gamma (IFN-) has a critical function in the noncytolytic elimination of several neurotropic viral infections, the signaling response to the cytokine is not characterized in primary... Read More ...
A couple of months ago, I started going to GriefShare classes (which are ran out of local churches all over the US. I highly recommend it!). I decided to go because I felt like life was going on as normal but I knew everything wasnt normal. It may have been because I spend a majority of my time at home with the kids and there wasnt the emotional room to grieve everyday and also care for the kids. I wasnt able to be available (or present) to them...so I decided I needed to set aside time for Noa, and thus, the classes. It has been so good. Its help put parameters on what Im experiencing and how it might be different in the light of our situation. It also has given some makers of growth and typical areas people might get stuck. And they just do a good job of covering many areas a grieving person will encounter. The first class I went to dealt with how family members grieve differently...and it brought deeper assurance that Justin not attending the class with me was ok...even good. And ...
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Discover Lifes page about the biology, natural history, ecology, identification and distribution of Childs, Ken I_KEN/0002 -- Discover Life
Lala Kent has always been candid about her struggles with substance abuse, but last month she opened up about her struggle with alcoholism -what shes doing about it. Five months ago, I came to the realization that I am an alcoholic, and I am now a friend of Bill W., Kent said on Instagram. In an interview with
人乳腺癌体细胞突变PCR芯片适用于快速、准确的检测和研究乳腺癌的常见基因突变,包括:AKT1, APC, BRAF, CDH1, CTNNB1, HRAS, NRAS, KRAS, PIK3CA和TP53。对上述基因突变的深入研究有助于科研工作者更好的
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What can CDH cause?. Babies with CDH can have severe breathing problems, as well as pulmonary hypertension, a condition that makes it difficult for blood to circulate through the lungs. The severity of symptoms and the babys survival depends on the size of the defect in the diaphragm, the amount and types of organs pushing up into the chest and the amount of lung tissue that can develop. Problems with other organs can also affect the survival of babies with CDH.. What can families expect?. When CDH is diagnosed prenatally, you will be referred to a perinatologist, a pediatric surgeon and a neonatologist to discuss what type of CDH your baby has and what to expect. Delivery will be planned at a tertiary medical center with direct transfer to Rady Childrens Hospital-San Diego.. Sometimes CDH is diagnosed after the baby is born. Your baby will be transferred directly to Rady Childrens as soon as the diagnosis is made.. What happens after delivery?. Most babies with CDH will need a breathing tube ...
Introduction Down-regulation of E-cadherin (CDH1) and epithelial-mesenchymal transition (EMT) are considered critical events for invasion and metastasis of colorectal carcinoma. Here we tested whether the important regulators of E-cadherin expression SNAI1 and TWIST1 are already detectable in human colorectal adenomas. Methods RNA was extracted from a set of randomly selected formalin-fixed and paraffin-embedded (FFPE) colorectal adenomas (n = 41) and normal colon mucosa (n = 10). Subsequently mRNA expression of CDH1, CDH2, SNAI1 and TWIST1 was analysed by quantitative RT-PCR analysis. CDH1 as well as SNAI1 protein expression were assessed by immunohistochemistry (IHC). Results SNAI1 mRNA was expressed in 78% (n = 32/41), TWIST1 mRNA in 41% (n = 17/41) and CDH2 mRNA in 41% (n = 17/41) of the colorectal adenoma tissue, while normal colon mucosa was negative for these transcription factors. We found a significant correlation between reduced CDH1 and the presence of SNAI1 mRNA expression and for
Looking for online definition of CDH13 in the Medical Dictionary? CDH13 explanation free. What is CDH13? Meaning of CDH13 medical term. What does CDH13 mean?
Looking for online definition of CDH5 in the Medical Dictionary? CDH5 explanation free. What is CDH5? Meaning of CDH5 medical term. What does CDH5 mean?
CDH1 research is a relatively new area in certain respects. In 1999, a medical researcher in New Zealand named Dr. Parry Guilford and his colleagues discovered the connection between CDH1 mutations and cancers of the stomach and breasts. Despite it being a relatively recent discovery, searching for information about CDH1 research returns a lot of information.. Using publicly available information, I am researching the landscape of research on issues relating to CDH1 mutation carriers. Typically, when doing this kind of work in the past, I have used Google Scholar and the United States Patent Office patent and patent application databases. Those are great resources for learning more about research in many areas of medical research. Using those tools to do some preliminary searching about CDH1 issues, it seems that researchers are searching for ways to improve methods for detecting early signs of diffuse stomach cancer and lobular breast cancer. Also, they are exploring drug development and other ...
Cadherin-related 23 (CDH23) is an adhesive protein very important to hearing and vision while CAMSAP3/Marshalin is definitely a microtubule (MT) minus-end binding protein that regulates MT systems. a process that will require a tubulin-binding site known as CKK. We further determined a conserved N-terminal area of CDH23-C that binds towards the CKK site. This CKK binding theme (CBM) can be next to the site that interacts with harmonin a binding partner of Lacidipine CDH23 implicated in deafness. As the human being Usher Symptoms 1D-connected mutation CDH23 R3175H maps towards the CBM we developed a matched up mutation in mouse CDH23-C at R55H. Both and assays reduced the power of CDH23-C to connect to CAMSAP3/Marshalin indicating that the discussion between CDH23 and CAMSAP3/Marshalin takes on a vital part in Lacidipine hearing and eyesight. Collectively our data claim that CDH23-C can be a CAMSAP3/Marshalin-binding proteins that can alter MT systems indirectly through its discussion with ...
CDH17 Antibody (monoclonal) (M01), Mouse monoclonal antibody raised against a partial recombinant CDH17. validated in WB, IHC, E (AT1472a), Abcepta
Germline mutations in the CDH1 (E-cadherin gene) gene have been reported in families with a hereditary predisposition to breast cancer and gastric cancer. Sequencing and deletion/duplication analyses of the CDH1 gene will identify individuals at risk for CHD1-related cancers ...
Prenatal therapy to stimulate lung growth. The critical point in fetuses diagnosed with CDH is not the defect in their diaphragm, but the lung volume available at birth. Indeed, when inside the womb, the baby gets its oxygen from the mother through the placenta. At birth however, a baby needs his/her own lungs to breathe and to provide sufficient oxygen to the other organs. Although the human lung can still grow a lot after birth, its size might not be enough to provide sufficient oxygen uptake in the first few days of life. Therefore, intensive neonatal care might for some babies not be enough to help them survive. As a consequence, if one would like to improve the prognosis for these very severe cases of CDH, one would need to do something before birth to improve lung growth and to provide these babies with a better start.. For more than 25 years researchers have been exploring this path. Initially they tried to provide space for the developing lung in the way it is done after birth: surgeons ...
E-cadherin, cadherin-1, uvomorulin, epithelial cadherin WP HGNC PO Definition: Tumor suppressor gene CDH1 is at 16q22.1 Calcium-dependent (...)
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Promoting complex/cyclosome (APC/C) associates with cadherin 1 (CDH1), acting as a ubiquitin ligase to down-regulate GA . The APC/C DH1 complicated targets
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Polyclonal antibody for VE CADHERIN/CDH5 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. VE CADHERIN/CDH5 information: Molecular Weight: 87528 MW; Subcellular Localization: Cell junction . Cell membrane ; Sin
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2004). "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Curr. Biol. 13 (17): ... The encoded protein is required for proper protein ubiquitination function of APC/C and for the interaction of APC/C with ... "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Curr. Biol. 13 (17): 1459-68. ... Gmachl M, Gieffers C, Podtelejnikov AV, Mann M, Peters JM (August 2000). "The RING-H2 finger protein APC11 and the E2 enzyme ...
2004). "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Curr. Biol. 13 (17): ... "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Curr. Biol. 13 (17): 1459-68. ... Gmachl, M; Gieffers C; Podtelejnikov A V; Mann M; Peters J M (August 2000). "The RING-H2 finger protein APC11 and the E2 enzyme ... A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition ...
2004). "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Curr. Biol. 13 (17): ... 2002). "APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting ... "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Curr. Biol. England. 13 (17): ... "APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex". Mol ...
2004). "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Curr. Biol. 13 (17): ... 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. Bibcode: ... "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Curr. Biol. 13 (17): 1459-68. ... Gmachl, M; Gieffers C; Podtelejnikov A V; Mann M; Peters J M (August 2000). "The RING-H2 finger protein APC11 and the E2 enzyme ...
CDH1 - E-cadherin (epithelial): E-cadherins are found in epithelial tissue; not to be confused with the APC/C activator protein ... Berx G, Becker KF, Höfler H, van Roy F (1998). "Mutations of the human E-cadherin (CDH1) gene". Human Mutation. 12 (4): 226-37 ... Moran CJ, Joyce M, McAnena OJ (April 2005). "CDH1 associated gastric cancer: a report of a family and review of the literature ... Cadherins are a class of type-1 transmembrane proteins, and they are dependent on calcium (Ca2+) ions to function, hence their ...
The gene mutated in HDGC, CDH1, codes for the E-Cadherin protein. This protein serves numerous functions in cell to cell ... CDH1) located on chromosome 16. Individuals who inherit an inactive copy of the CDH1 gene are at significantly elevated risk ... Although CDH1 is by far the most common gene associated with HDGC, around 11% of cases arise in individuals who are negative ... Mutations in CDH1 are also associated with high risk of lobular breast cancers, and may be associated with a mildly elevated ...
In that species, one important target is Cdh1/Hct1. Cdh1 associates with the APC and leads to APC activity (anaphase promoting ... "The structure of the cell cycle protein Cdc14 reveals a proline-directed protein phosphatase". EMBO J. 22 (14): 3524-3535. doi: ... Also, while the protein regulates the Cdk1 ortholog of S. pombe, this occurs through a process unlike that of S. cerevisiae; it ... Cdc14 and Cdc14 are a gene and its protein product respectively. Cdc14 is found in most of the eukaryotes. Cdc14 was defined by ...
"TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Current Biology. 13 (17): 1459- ... This protein and two other APC complex proteins, CDC23 and CDC27, contain a tetratricopeptide repeat (TPR), a protein domain ... This gene encodes a component protein of the APC complex, which is composed of eight proteins and functions as a protein ... Cell division cycle protein 16 homolog is a protein that in humans is encoded by the CDC16 gene. ...
2004). "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Curr. Biol. 13 (17): ... 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. Bibcode: ... "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Curr. Biol. 13 (17): 1459-68. ... "APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex". Mol ...
"TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Current Biology. 13 (17): 1459- ... "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Current Biology. 13 (17): 1459- ... "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. Bibcode:2005Natur. ...
A closely related protein, Cdc20homologue-1 (Cdh1) plays a complementary role in the cell cycle. CDC20 appears to act as a ... CDC20 is a protein related to the beta subunit of heterotrimeric G proteins. Near its C-terminus it contains seven WD40 repeats ... Securin is a protein that inhibits separase, which in turn inhibits cohesin, a protein that holds sister chromatids together. ... Importantly, cyclin accumulation is also prevented by Cdh1. CDC20-homologue 1 (Cdh1) plays a complementary role to CDC20 in ...
Seki A, Fang G (2007). "CKAP2 is a spindle-associated protein degraded by APC/C-Cdh1 during mitotic exit". J. Biol. Chem. 282 ( ... Cytoskeleton-associated protein 2 is a protein that in humans is encoded by the CKAP2 gene. Human CKAP2 gene, the cDNA of which ... 2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs ... "Entrez Gene: CKAP2 cytoskeleton associated protein 2". Alternative titles: TUMOR- AND MICROTUBLE-ASSOCIATED PROTEIN; TMAP, LB1 ...
"TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Current Biology. 13 (17): 1459- ... Fizzy-related protein homolog, also known as hCDH1, is a protein that in humans is encoded by the FZR1 gene. FZR1 has been ... Zhou Y, Ching YP, Chun AC, Jin DY (April 2003). "Nuclear localization of the cell cycle regulator CDH1 and its regulation by ... Kraft C, Vodermaier HC, Maurer-Stroh S, Eisenhaber F, Peters JM (May 2005). "The WD40 propeller domain of Cdh1 functions as a ...
2004). "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Curr. Biol. 13 (17): ... "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Curr. Biol. 13 (17): 1459-68. ... 2000). "The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase- ... The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby ...
... has been shown to interact with: Beta-catenin, CDH1, Catenin (cadherin-associated protein), alpha 1, Nephrin and ... Cadherin-3, also known as P-Cadherin, is a protein that in humans is encoded by the CDH3 gene. This gene is a classical ... The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a ... Herrenknecht K, Ozawa M, Eckerskorn C, Lottspeich F, Lenter M, Kemler R (Oct 1991). "The uvomorulin-anchorage protein alpha ...
... has been shown to interact with Cdh1. FOX proteins GRCh38: Ensembl release 89: ENSG00000111206 - Ensembl, May 2017 GRCm38 ... Forkhead box protein M1 is a protein that in humans is encoded by the FOXM1 gene. The protein encoded by this gene is a member ... FOXM1+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text from ... Laoukili J, Alvarez-Fernandez M, Stahl M, Medema RH (September 2008). "FoxM1 is degraded at mitotic exit in a Cdh1-dependent ...
Cdc20 and Cdh1 are the two activators of particular importance to the cell cycle. These proteins target the APC/C to specific ... Cdh1 does not require APC to be phosphorylated in order to bind, in fact, phosphorylation of Cdh1 by Cdks prevents it from ... It is important to note that the conserved area of the propeller of Cdh1 is much larger than that of Cdc20, allowing Cdh1 to ... Evidence shows that APC3 and APC7 serve to recruit Cdh1 to the anaphase promoting complex. This further supports that Cdh1 is ...
CDH1 protein, human Medical Subject Headings (MeSH) na Biblioteca Nacional de Medicina dos EUA. ... Oneyama C, Nakano H, Sharma SV (March 2002). "UCS15A, a novel small molecule, SH3 domain-mediated protein-protein interaction ... "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Curr. Biol. 13 (17): 1459-68. ... Control xenético e epixenético de CDH1[editar , editar a fonte]. Varias proteínas como SNAI1/SNAIL,[53][54] ZFHX1B/SIP1,[55] ...
It is hypothesized that Clb5 and Clb6 are somewhat resistant to APC-Cdh1 degradation since they are primarily regulated by APC- ... Clb5, in particular, has unique hydrophobic section of amino acids that allows specific interactions with proteins in the pre- ... Finally, Clb5-Cdk1 has been shown to be important for the phosphorylation of Cdh1 in yeast, and Clb5 destruction promotes ... An important regulatory event during G1 is the inactivation of the anaphase promoting complex (APC-Cdh1). Clb5 and Clb6 ...
"TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Current Biology. 13 (17): 1459- ... a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins ... Cell division cycle protein 27 homolog is a protein that in humans is encoded by the CDC27 gene. The protein encoded by this ... This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly ...
"TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Current Biology. 13 (17): 1459- ... a protein domain important for protein-protein interaction. CDC23 has been shown to interact with CDC27. GRCh38: Ensembl ... This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly ... The CDC23 protein shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression ...
2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. ... CHD1 contains two tandem N-terminal chromodomains, a SNF2-related domain, a helicase C domain, CDH1/2 SANT-Helical linker, and ... CHD1 is a member of a large family of CHD nucleosome remodelers, though yeast has only one CHD protein, called Chd1. Humans and ... The Chromodomain-Helicase DNA-binding 1 is a protein that, in humans, is encoded by the CHD1 gene. CHD1 is a chromatin ...
... the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain ... Cadherin-16 is a protein that in humans is encoded by the CDH16 gene. This gene is a member of the cadherin superfamily, genes ... 2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and ... Baudry D, Jeanpierre C (2000). "Assignment of E-cadherin (CDH1) and KSP-cadherin (CDH16) to chromosome 16q22.1 by radiation ...
Beta-transducin repeat-containing protein (βTRCP) is an FBP that targets emi1-an APC/C-Cdh1 inhibitor-and wee1 for degradation ... Skp1 - Skp1 is an adaptor protein that is essential for the recognition and binding of F-box proteins. Cullin (CUL1) - Cullin ... The SCF complex also marks various other cellular proteins for destruction. SCF contains a variable F-box protein and three ... Instead, FBP affinity for protein substrates is regulated through cyclin-CDK-mediated phosphorylation of target proteins. ...
F-box only protein 5 is a protein that in humans is encoded by the FBXO5 gene. This gene encodes a member of the F-box protein ... Cdh1)". Nature Cell Biology. 4 (5): 358-66. doi:10.1038/ncb785. PMID 11988738. S2CID 25403043. Li Z, Hu CY, Mo BQ, Xu JD, Zhao ... and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this ... The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box ...
Swope D, Cheng L, Gao E, Li J, Radice GL (Mar 2012). "Loss of cadherin-binding proteins β-catenin and plakoglobin in the heart ... Plakoglobin has been shown to interact with: APC, CTNNA1, CTNNB1, CDH1, CDH2, CDH3, CDH5, DSG2, DSP, MUC1, PKP2, PTPkappa ( ... Plakoglobin, also known as junction plakoglobin or gamma-catenin, is a protein that in humans is encoded by the JUP gene. ... Rubinfeld B, Souza B, Albert I, Munemitsu S, Polakis P (Mar 1995). "The APC protein and E-cadherin form similar but independent ...
... a phosphatase that counteracts Cdk1 via activation of Cdh1 and Swi5, a transcriptional activator of Sic1 proteins. While ... promotes formation of APC in association with Cdh1 (APC-Cdh1) to degrade Clb2s. Cdc20 and Cdh1, which are the activators of APC ... This allows for the transcription of S phase genes encoding for proteins that amplify the G1 to S phase switch. Many different ... This action is controlled by the Esp1 protein, which is tightly bound by the anaphase inhibitor Pds1 that is destroyed by the ...
... a phosphatase that counteracts Cdk1 via activation of Cdh1 and Swi5, a transcriptional activator of Sic1 proteins. While ... promotes formation of APC in association with Cdh1 (APC-Cdh1) to degrade Clb2s. Cdc20 and Cdh1, which are the activators of APC ... The double-negative feedback loop, formed by APC-Cdh1 and Sic1, is required to maintain low Clb2-Cdk1 activity because Clb2 ... Without Cdk1-Clb2 complexes to phosphorylate proteins that are involved in spindle dynamics such as Sli15, Ase1, and Ask1, ...
CDH1, CDH13), are inactivated by this epigenetic lesion. Hypermethylation is linked to methyl-binding proteins, DNA ...
The N-terminal region mediates binding of Hs-BUB1 to the mitotic kinetochore protein blinkin (a protein also commonly referred ... In turn APC/C, now in complex with Cdh1, also acts on Bub1 by priming it for degradation to exit mitosis. In addition, ... Bub1 is a serine/threonine protein kinase first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound ... The protein kinase Bub1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome ...
The protein degradation processEdit. Ribbon diagram of ubiquitin, the highly conserved protein that serves as a molecular tag ... Cdh1) ubiquitin ligase". Nature. 428 (6979): 190-3. doi:10.1038/nature02330. PMID 15014502.. ... Proteasomes are protein complexes which degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks ... Proteins are tagged for degradation with a small protein called ubiquitin. The tagging reaction is catalyzed by enzymes called ...
Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... a b Proto-Oncogene+Proteins+c-sis at the US National Library of Medicine Medical Subject Headings (MeSH) ... Hannink M, Donoghue DJ (1989). "Structure and function of platelet-derived growth factor (PDGF) and related proteins". Biochim ... The first engineered dominant negative protein was designed to inhibit PDGF [29] ...
protein binding. • calcium ion binding. • metal ion binding. Cellular component. • integral component of membrane. • membrane. ... an EGF-like domain and a complement-binding protein-like domains (same as complement regulatory proteins: CRP) having short ... calcium-dependent protein binding. • glycosphingolipid binding. • fucose binding. • carbohydrate binding. • sialic acid binding ... P-selectin is a protein that in humans is encoded by the SELP gene.[5] ...
identical protein binding. • cytoskeletal protein binding. • protein homodimerization activity. • cadherin binding. Cellular ... Cadherin-8 is a protein that in humans is encoded by the CDH8 gene.[5][6][7] ... Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a ... This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium- ...
protein binding. • cyclin-dependent protein serine/threonine kinase inhibitor activity. • ubiquitin protein ligase binding. • ... cyclin-dependent protein serine/threonine kinase activity. • protein kinase inhibitor activity. • protein kinase binding. • ... This article is about the p21Cip1 protein. For the p21/ras protein, see Ras (protein). For other uses, see P21 (disambiguation) ... protein stabilization. • positive regulation of cyclin-dependent protein kinase activity. • regulation of transcription from ...
... or uvomorulin is a protein that in humans is encoded by the CDH1 gene.[5] CDH1 has also been designated as CD324 (cluster of ... Genetic and epigenetic control of CDH1[edit]. Several proteins such as SNAI1/SNAIL,[58][59] ZFHX1B/SIP1,[60] SNAI2/SLUG,[61][62 ... Inactivation of CDH1 in 50% of diffuse gastric carcinomas.[56]. *Complete loss of E-cadherin protein expression in 84% of ... protein binding. • ankyrin binding. • gamma-catenin binding. • beta-catenin binding. • GTPase activating protein binding. • ...
There are many genes and proteins that contribute to the formation and development of the central nervous system, many of which ... and various cadherin coding genes like CDH1 and CDH2.[30] ... of expression of these genes and their corresponding proteins.[ ... One area deals with molecular processes and the function of certain proteins, often in conjunction with cell signaling and ...
protein kinase activity. • kinase activity. • protein binding. • protein tyrosine kinase activity. • ATP binding. • Ras guanyl- ... membrane protein proteolysis. • phosphorylation. • transmembrane receptor protein tyrosine kinase signaling pathway. • positive ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... "The Ret receptor protein tyrosine kinase associates with the SH2-containing adapter protein Grb10". J. Biol. Chem. 270 (37): ...
A vinculina interacciona con SORBS1,[12] CDH1[13][14] e a paxilina.[15][16][17] ... 1995). "Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL". J. Biol. Chem. 270 (10): ... Geiger,B. 130K Protein from Chicken Gizzard - Its Localization at the Termini of Microfilament Bundles in Cultured Chicken- ... Critchley DR (2005). "Cytoskeletal proteins talin and vinculin in integrin-mediated adhesion". Biochem. Soc. Trans. 32 (Pt 5): ...
Vascular cell adhesion protein 1 also known as vascular cell adhesion molecule 1 (VCAM-1) or cluster of differentiation 106 ( ... The VCAM-1 protein mediates the adhesion of lymphocytes, monocytes, eosinophils, and basophils to vascular endothelium. It also ... Primarily, the VCAM-1 protein is an endothelial ligand for VLA-4 (Very Late Antigen-4 or integrin α4β1) of the β1 subfamily of ... Proteins: clusters of differentiation (see also list of human clusters of differentiation) ...
protein binding. • enzyme binding. • metal ion binding. • identical protein binding. • ubiquitin protein ligase binding. • p53 ... protein ubiquitination. • negative regulation of protein processing. • establishment of protein localization. • response to ... protein deubiquitination. • protein sumoylation. • transcription factor catabolic process. • protein autoubiquitination. • ... Mouse double minute 2 homolog (MDM2) also known as E3 ubiquitin-protein ligase Mdm2 is a protein that in humans is encoded by ...
The gene that codes for the SDHB protein is nuclear, not mitchondrial DNA. However, the expressed protein is located in the ... "Cardiac Organellar Protein Atlas Knowledgebase (COPaKB).. *^ Sun, F; Huo, X; Zhai, Y; Wang, A; Xu, J; Su, D; Bartlam, M; Rao, Z ... Click on genes, proteins and metabolites below to link to respective articles. [§ 1] ... protein binding. • oxidoreductase activity. • electron carrier activity. • 2 iron, 2 sulfur cluster binding. • 3 iron, 4 sulfur ...
Helicobacter pylori CagA protein can be tyrosine phosphorylated in gastric epithelial cells. „J Exp Med". 191 (4), s. 593-602, ... Ponadto u kobiet z mutacją CDH1 przeprowadza się profilaktyczne badania piersi na zasadach podobnych jak w dziedzicznym raku ... A study of HER2 gene amplification and protein expression in gastric cancer. „J Clin Pathol". 63 (9), s. 839-842, Sep 2010. DOI ... CDH1)[47]. Mutacja tego genu jest stwierdzana w 30-50% przypadków tej choroby. Średni wiek, przy którym pojawia się rak żołądka ...
protein dimerization activity. • transcription factor activity, sequence-specific DNA binding. • protein complex binding. • ... protein complex. • nucleolus. • nucleus. • nuclear chromatin. Biological process. • Notch signaling pathway. • chromatin ... "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3: 89. PMC 1847948 ... protein deubiquitination. • positive regulation of transcription, DNA-templated. • positive regulation of telomerase activity. ...
"The stomach cancer proteome - The Human Protein Atlas". www.proteinatlas.org.. *^ Uhlen, Mathias; Zhang, Cheng; Lee, Sunjae; ... A genetic risk factor for gastric cancer is a genetic defect of the CDH1 gene known as hereditary diffuse gastric cancer (HDGC ... The CDH1 gene, which codes for E-cadherin, lies on the 16th chromosome.[41] When the gene experiences a particular mutation, ... arise from an inherited abnormal CDH1 gene. Genetic testing and treatment options are available for families at risk.[44] ...
Oneyama C, Nakano H, Sharma SV (Mar 2002). "UCS15A, a novel small molecule, SH3 domain-mediated protein-protein interaction ... CDH1,[18][68][84][85][86][87][88][89][90][91][92][93][94][95][96][97][98][99][100][101][102][103][104] ... "Entrez Gene: catenin (cadherin-associated protein)".. *↑ Nelson WJ (Apr 2008). "Regulation of cell-cell adhesion by the ... He Y, Yan H, Dong H, Zhang P, Tang L, Qiu X, Wu M, Wang H (Apr 2005). "Structural basis of interaction between protein tyrosine ...
The protein is a determinant for the Indian blood group system. *CD44, along with CD25, is used to track early T cell ... protein binding. • hyaluronic acid binding. • cytokine receptor activity. Cellular component. • integral component of membrane ... Sun CX, Robb VA, Gutmann DH (Nov 2002). "Protein 4.1 tumor suppressors: getting a FERM grip on growth regulation". Journal of ... Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor ...
Since many proteins are structurally similar, especially within the same protein family, Broad-spectrum inhibitors can't always ... A few of the common genes frequently observed are APC, CDH1, CDKN2A, MGMT, and RASSF1A (a tumor suppressor). In the cases of ... CXCR4 is a protein that acts as a coreceptor for the entry of HIV. It has been developed as a drug target for anti-HIV therapy ... CYP1A1 is a protein that is well known for its role in chemical compounds and drug metabolism. A study in prostate cancer ...
... novel SH2-containing protein family), and other proteins such as the Id family of helix-loop-helix proteins. In terms of post- ... CDH1 subunit of the APC complex recognizes NEDD9 and regulates ubiquitination and subsequent degradation of NEDD9. NEDD9 is ... Neural precursor cell expressed developmentally down-regulated protein 9 (NEDD-9) is a protein that in humans is encoded by the ... NEDD9 is a noncatalytic scaffolding protein that contains docking sites for proteins involved in multiple signal transduction ...
CTNND1 has been shown to interact with: Beta-catenin, CDH1, CDH2, Collagen, type XVII, alpha 1, Cortactin, FYN, MUC1, Nephrin, ... p120, and called catenin delta-1 is a protein that in humans is encoded by the CTNND1 gene. This gene encodes a member of the ... The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 4 (2): 141-50. ... "Entrez Gene: CTNND1 catenin (cadherin-associated protein), delta 1". Schackmann, R. C. J.; Tenhagen, M.; van de Ven, R. A. H.; ...
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Protein tyrosine phosphatases ... PTPRK has been shown to interact with: Beta-catenin, E-cadherin (CDH-1), Epidermal growth factor receptor (EGFR), HER2, ... "Galectin-3 binding protein promotes cell motility in colon cancer by stimulating the shedding of protein tyrosine phosphatase ... "Identification of target proteins of N-acetylglucosaminyl transferase V in human colon cancer and implications of protein ...
HRG Thrombophilia due to protein C deficiency, autosomal dominant; 176860; PROC Thrombophilia due to protein C deficiency, ... CDH1 Gastric cancer, somatic; 137215; APC Gastric cancer, somatic; 137215; CASP10 Gastric cancer, somatic; 137215; ERBB2 ... SLITRK1 Trifunctional protein deficiency; 609015; HADHA Trifunctional protein deficiency; 609015; HADHB Trigonocephaly; 190440 ... G6PC3 D-bifunctional protein deficiency; 261515; HSD17B4 De la Chapelle dysplasia; 256050; SLC26A2 De Sanctis-Cacchione ...
The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. ... FYN has been shown to interact with: ADD2, BCAR1, C-Raf, CBLC, CD36, CD44, CDH1, CHRNA7, CTNND1, CBL, CSF1R, DLG4, Dystroglycan ... Tyrosine phosphorylation of target proteins by Fyn serves to either regulate target protein activity, and/or to generate a ... Oneyama C, Nakano H, Sharma SV (March 2002). "UCS15A, a novel small molecule, SH3 domain-mediated protein-protein interaction ...
... and Fbxos containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by ... Cdh1) in G1 phase". The EMBO Journal. 27 (4): 679-91. doi:10.1038/emboj.2008.6. PMC 2262036. PMID 18239684. Bashir T, Pagan JK ... The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), ... Yam CH, Ng RW, Siu WY, Lau AW, Poon RY (January 1999). "Regulation of cyclin A-Cdk2 by SCF component Skp1 and F-box protein ...
Amon's team demonstrated that CDC20 is the target protein in the spindle checkpoint during mitosis. Amon's more recent work has ... Vistinin, Rosella; Prinz, Susanne; Amon, Angelika (1997). "CDC20 and CDH1: A Family of Substrate-Specific Activators of APC- ... More specifically, she demonstrated that CDC28 protein kinase is not required for the metaphase to anaphase transition and CLB2 ... and interference with protein biosynthesis. Amon has also examined trisomy in the mouse as a model of mammalian cell growth and ...
CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent ... Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in ... to allow unambiguous identification of a protein.,p>,a href=/help/protein_names target=_top>More...,/a>,/p>Protein namesi. ... Protein. Similar proteins. Organisms. Length. Cluster ID. Cluster name. Size. Q9R0T4. P09803. Q4KML8. UPI00017BDAFC. ...
CCS52A1 encodes a CDH1/FZR-like protein, a class of proteins that function as activators of the anaphase-promoting complex. ... the CCS52A proteins seem likely to be the most similar in function to CDH1/FZR proteins (Fülop et al. 2005). Arabidopsis has ... CDH1/FZR proteins are substrate-specific activators of the APC/C that regulate M/G1-phase-specific proteolysis and are required ... During the mitotic cell cycle, APC/CCDH1 targets proteins containing the Dbox motif, and in some cases other amino acid ...
Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months.. Please prevent ... Cadherin-E protein (100µg/1ml) in 50mM Tris-HCl, pH7.5 and 10mM L-glutathione (reduced). ... Epithelial cadherin, E-cadherin, Uvomorulin, Cadherin-1, CAM 120/80, CD324 antigen, CDH1, CDHE, UVO, ECAD, LCAM, Arc-1, CD324, ...
E-Cadherin Human Recombinant produced in HEK cells is a secreted protein with the sequence of Human E-Cadherin (amino acids ... The CDH1 protein was lyophilized from a 0.2µm filtered solution in PBS, pH7.4. ... It is recommended to reconstitute the lyophilized CDH1 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further ... For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).. Please prevent freeze-thaw cycles. ...
Expression of CDH1 (CD324, UVO, uvomorulin) in vagina tissue. Antibody staining with HPA004812, CAB000087, CAB028364, CAB072855 ... Protein evidence (Ezkurdia et al 2014). Protein evidence (Kim et al 2014). RAS pathway related proteins. Ribosomal proteins. ... Plasma proteins. Potential drug targets. Predicted intracellular proteins. Predicted membrane proteins. Predicted secreted ... Protein expressioni. The protein expression bar, with the units not detected (n), low (l), medium (m) and high (h), is based on ...
The Cdc20 (Fzy)/Cdh1-related protein, Cort, cooperates with Fzy in cyclin destruction and anaphase progression in meiosis I and ... The Cdc20 (Fzy)/Cdh1-related protein, Cort, cooperates with Fzy in cyclin destruction and anaphase progression in meiosis I and ... The Cdc20 (Fzy)/Cdh1-related protein, Cort, cooperates with Fzy in cyclin destruction and anaphase progression in meiosis I and ... The Cdc20 (Fzy)/Cdh1-related protein, Cort, cooperates with Fzy in cyclin destruction and anaphase progression in meiosis I and ...
cell cycle proteins:phospho-APC/C:Cdh1 complex [cytosol] (Mus musculus) * multiubiquitinated cell cycle protein:APC/C:Cdh1 ... multiubiquitinated cell cycle protein:APC/C:Cdh1 complex [cytosol] (Mus musculus) * cell cycle proteins:phospho-APC/C:Cdh1 ... Association of cell cycle proteins with the APC/C:Cdh1 complex (Mus musculus) * cell cycle proteins:phospho-APC/C:Cdh1 complex ... APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 (Mus musculus) * ...
... or uvomorulin is a protein that in humans is encoded by the CDH1 gene.[5] CDH1 has also been designated as CD324 (cluster of ... Genetic and epigenetic control of CDH1[edit]. Several proteins such as SNAI1/SNAIL,[58][59] ZFHX1B/SIP1,[60] SNAI2/SLUG,[61][62 ... Inactivation of CDH1 in 50% of diffuse gastric carcinomas.[56]. *Complete loss of E-cadherin protein expression in 84% of ... protein binding. • ankyrin binding. • gamma-catenin binding. • beta-catenin binding. • GTPase activating protein binding. • ...
Edges represent protein-protein associations. associations are meant to be specific and meaningful, i.e. proteins jointly ... CDH1. YNL172W. YGL003C. Largest subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ... CDH1. YFR028C. YGL003C. Protein phosphatase required for mitotic exit; required for rDNA segregation, cytokinesis, meiosis I ... Network nodes represent proteins. splice isoforms or post-translational modifications are collapsed, i.e. each node represents ...
DictionaryProtein expressionCDH1. Cdh1. Melanoma (SSM) in skin showing the expression of E-cadherin (CDH1) in both normal cells ... Protein evidence (Ezkurdia et al 2014). Protein evidence (Kim et al 2014). RAS pathway related proteins. Ribosomal proteins. ... Plasma proteins. Potential drug targets. Predicted intracellular proteins. Predicted membrane proteins. Predicted secreted ... E-cadherin (CDH1) - Marker for non-neural epithelial cells. The cadherins are a large family of transmembrane proteins that are ...
CELL DIVISION CYCLE PROTEIN 23 HOMOLOG. C, P. 597. Homo sapiens. Mutation(s): 0 Gene Names: CDC23, ANAPC8. ... Definition of Cdh1 interactions with the APC/C indicates how they are antagonized by Cdh1 phosphorylation. The structure of the ... FUSION PROTEIN - UBIQUITIN-CONJUGATING ENZYME E2 C, UBIQUITIN-CONJUGATING ENZYME E2 S. Q. 162. Homo sapiens. Mutation(s): 0 ... Atomic Structure of the Apc/C and its Mechanism of Protein Ubiquitination.. Chang, L., Zhang, Z., Yang, J., Mclaughlin, S.H., ...
The adaptor protein of the anaphase promoting complex Cdh1 is essential in maintaining replicative lifespan and in learning and ... The adaptor protein of the anaphase promoting complex Cdh1 is essential in maintaining replicative lifespan and in learning and ... The adaptor protein of the anaphase promoting complex Cdh1 is essential in maintaining replicative lifespan and in learning and ... The adaptor protein of the anaphase promoting complex Cdh1 is essential in maintaining replicative lifespan and in learning and ...
Protein Mutation Frequency in Cancer. The lollipop plot above illustrates recurrent (observed in 3 or more out of 4440 TCGA ...
The CDH1 gene provides instructions for making a protein called epithelial cadherin or E-cadherin. Learn about this gene and ... The CDH1 gene provides instructions for making a protein called epithelial cadherin or E-cadherin. This protein is found within ... These CDH1 gene mutations are thought to result in a nonfunctional E-cadherin protein. A loss of functional E-cadherin in these ... CDH1 gene mutations that cause BCD syndrome are thought to result in an abnormal E-cadherin protein that is quickly broken down ...
Here, we show that in budding yeast the activation of APC(Cdh1) is controlled in part by destruction of t … ... The APC is activated by association with Cdc20 in midmitosis and Cdh1 in late mitosis and G1. ... The completion of mitosis depends on protein ubiquitination by the anaphase-promoting complex (APC). ... The completion of mitosis depends on protein ubiquitination by the anaphase-promoting complex (APC). The APC is activated by ...
APC/C associated with the Cdh1 protein (APC/CCdh1) delays the increase in Cdk1 activity, leading to germinal vesicle breakdown ... APC/C Cdh1 Enables Removal of Shugoshin-2 from the Arms of Bivalent Chromosomes by Moderating Cyclin-Dependent Kinase Activity ... GVBD). More surprisingly, by moderating the rate at which Cdk1 is activated following GVBD, APC/CCdh1 creates conditions ...
STRING v9.1: protein-protein interaction networks, with increased coverage and integration. Nucleic Acids Res. 41, D808-15 ( ... CDH1/2, this compartment contains the CTNNB1 regulation and its influence on CDH1 as well as angiogenesis and extracellular ... E2F1 is a remarkable example of a network hub as this protein interacts with many genes, proteins, and other transcription ... More specifically, we retrieved data on protein-protein interactions from STRING58 (v9.1) and HPRD59 (release 9). Transcription ...
APC, anaphase-promoting complex; C, cytokinesis; Cdc20, cell-division cycle protein 20; Cdh1, Cdc20 homologue-1; K, ... d) At E14.5 eGFP staining co-localizes with brain lipid-binding protein (BLBP; red), a known marker for RG cells in the VZ ( ... a) Schemes of subcellular localization of the eGFP-anillin fusion protein during the cell cycle and of the CAG-eGFP-anillin ... Here we overcome these limitations by generating an in vivo reporter system using the scaffolding protein anillin fused to ...
These different conformational changes inhibit specific RB-protein interactions. CDH1, CDC20 homologue 1; CDK, cyclin-dependent ... a , Schematic depiction of the F box protein S phase kinase-associated protein 2 (SKP2) recognizing phosphorylated p27. Binding ... a , Model of active and complexes with E2F and an L-X-C-X-E peptide (Protein Data Bank (PDB) codes: 2QDJ, 1GUX, 1N4M and 2AZE ... Molecular mechanisms underlying RB protein function.. Dick FA1, Rubin SM.. Author information. 1. London Regional Cancer ...
We next examined the ubiquitination of various securin proteins by APC/CCdh1. Consistent with a previous report (28), mutation ... 2011) Structures of APC/C(Cdh1) with substrates identify Cdh1 and Apc10 as the D-box co-receptor. Nature 470(7333):274-278. ... Protein Binding and Ubiquitination Assays.. Protein binding and ubiquitination assays were performed as described (39, 40). See ... Protein Purification and Crystallization.. Human Cdc20 proteins were expressed in insect cells and purified with affinity and ...
d, e) As controls for the specificity of APCcdh1- and pRB-mediated Skp2 protein turnover, SAOS-tetRB cells were transfected ... Figure 3: pRB-APCcdh1 association, ubiquitination of Skp2 and p27Kip1 protein accumulation are coordinated during pRB-mediated ... Cdh1 siRNA cotransfection served as a control. Immunoprecipitations from MG132-treated cell extracts were carried out using ... a) pRB was induced by tetracycline in a stably transfected SAOS2 cell line (SAOS-tetRB) and protein samples for immunoblotting ...
E-cadherin (CDH1) Antigen Profile Protein Summary This gene is a classical cadherin from the cadherin superfamily. The encoded ... Protein level used designations for CDH1 CAM 120/80 , E-Cadherin , cadherin 1, E-cadherin (epithelial) , cadherin-1 , calcium- ... Target Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) * Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) ... CDH1 Reactivity: Rat AA 24-704 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum ...
Identification of CDH1 germline missense mutations associated with functional inactivation of the E-cadherin protein in young ... CDH1) in hereditary diffuse gastric cancer (HDGC). We screened a series of 66 young gastric cancer probands for germline CDH1 ... cDNAs encoding either the wild-type protein or mutant forms of E-cadherin were stably transfected into CHO (Chinese hamster ...
Previous Document: Protein kinase A type II-? regulatory subunit regulates the response of prostate cancer cells to tax.... ... Interestingly, the Cyclin A/Cdk2 regulated APC/C(Cdh1) activity is selective for only a subset of Cdh1 targets including ... A or inhibition of Cdk2 during late S/early G2 phase maintains the G2 phase arrest by reducing Cdh1 transcript and protein ... Thus, a normal role for Cyclin A/Cdk2 during early G2 phase is to increase the level of Cdh1 which destabilises Claspin which ...
... to allow unambiguous identification of a protein.,p>,a href=/help/protein_names target=_top>More...,/a>,/p>Protein namesi. ... Protein-protein interaction databases. BioGRIDi. 36253, 153 interactors. ComplexPortali. CPX-25, U4/U6.U5 tri-snRNP complex. ... section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes ... Integrated resource of protein families, domains and functional sites. More...InterProi. View protein in InterPro. IPR004123, ...
Component #1: protein, Human ribosome nascent chain complex (CDH1-RNC) stalled .... Protein. Name: Human ribosome nascent chain ... Title: Structure of drug-like molecule stalled CDH1 ribosome nascent chain complex (CDH1-RNC) with PP tRNA. P-authors: Li W / ... Human ribosome nascent chain complex (CDH1-RNC) stalled by a drug-like molecule with PP tRNA:. Details. Source. Homo sapiens ( ... Name: Human ribosome nascent chain complex (CDH1-RNC) stalled by a drug-like molecule with PP tRNA. Number of components: 1. ...
Ajuba LIM proteins are snail. /. slug. corepressors required for neural crest. development in Xenopus., Langer EM, Feng Y, ... Papers associated with cdh1 (and morpholino). Search for cdh1 morpholinos using Textpresso #gene_search_suggest { position: ... The Xenopus LIM-homeodomain protein Xlim5. regulates the differential adhesion properties of early ectoderm. cells., Houston DW ... Vezatin, a protein associated to adherens junctions, is required for mouse blastocyst morphogenesis., Hyenne V, Louvet-Vallée S ...
Therefore we then turned to the CDH1−/− iMEFs.. Immunoblotting confirmed that CDH1−/− iMEFs were indeed Cdh1-deficient (Fig. 6B ... Cenp-F degradation occurs normally in Cdh1-null MEFS. To test the role of Cdh1 more rigorously, we immortalised CDH1+/+ and ... This not only initiates Cdh1-activated APC/C (APC/CCdh1)-mediated degradation but, because Cdc20 is itself a APC/CCdh1 ... 2000). Mitotic regulation of the APC activator proteins CDC20 and CDH1. Mol. Biol. Cell 11, 1555-1569. ...
Expression of Cdh1 and Ctnna1 was mainly observed in the luminal and glandular epithelia on Days 1 and 4 of pregnancy (Fig. 1A ... Taken together, these results suggest that AJ proteins contribute to uterine barrier functions by cell-cell adhesion to ensure ... Immunofluorescence study showed no change in epithelial expression patterns of uterine AJ proteins from Days 1 to 5 of ... The lack of changes in the patterns of AJ protein on these days eliminates the possibility that these proteins are markers of ...
D) Cdh1 binds Ube2S. HACdh1 or HACdh1ΔWD40 were precipitated from 293T cells, and co-purifying Ube2S was detected by Western ... 2006) Modification of proteins by ubiquitin and ubiquitin-like proteins. Annu Rev Cell Dev Biol 22:159-180. ... Bound Cdh1 was detected by Western blot. (F) Ube2S binds Cdh1. 35S-Cdh1 was incubated with immobilized MBPUbe2S or the ... Bound Cdh1 was detected by autoradiography. (G) Ube2S directly binds Cdh1. HisCdh1 purified to homogeneity was incubated with ...
  • Cadherin-E protein (100µg/1ml) in 50mM Tris-HCl, pH7.5 and 10mM L-glutathione (reduced). (prospecbio.com)
  • E-Cadherin Human Recombinant produced in HEK cells is a secreted protein with the sequence of Human E-Cadherin (amino acids Asp155-Ile707) and fused to a 6xHis tag at the C-terminus. (prospecbio.com)
  • Lyophilized E-Cadherin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CDH1 should be stored at 4°C between 2-7 days and for future use below -18°C. (prospecbio.com)
  • Cadherin-1 also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin is a protein that in humans is encoded by the CDH1 gene . (wikipedia.org)
  • The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region, and a highly conserved cytoplasmic tail . (wikipedia.org)
  • Complete loss of E-cadherin protein expression in 84% of lobular breast carcinomas. (wikipedia.org)
  • The CDH1 gene provides instructions for making a protein called epithelial cadherin or E-cadherin. (medlineplus.gov)
  • E-cadherin belongs to a family of proteins called cadherins whose function is to help neighboring cells stick to one another (cell adhesion) to form organized tissues. (medlineplus.gov)
  • Another protein called p120-catenin, produced from the CTNND1 gene, helps keep E-cadherin in its proper place in the cell membrane, preventing it from being taken into the cell through a process called endocytosis and broken down prematurely. (medlineplus.gov)
  • E-cadherin is one of the best-understood cadherin proteins. (medlineplus.gov)
  • Interactions between the E-cadherin and p120-catenin proteins, in particular, are thought to be important for normal development of the head and face (craniofacial development), including the eyelids and teeth. (medlineplus.gov)
  • E-cadherin also acts as a tumor suppressor protein, which means it prevents cells from growing and dividing too rapidly or in an uncontrolled way. (medlineplus.gov)
  • Researchers believe that the resulting loss of E-cadherin protein may allow breast cells to grow and divide unchecked, leading to a cancerous tumor. (medlineplus.gov)
  • The mutations that cause HDGC often lead to the production of an abnormally short, nonfunctional version of the E-cadherin protein or lead to the production of a protein with an altered structure. (medlineplus.gov)
  • CDH1 gene mutations that cause BCD syndrome are thought to result in an abnormal E-cadherin protein that is quickly broken down. (medlineplus.gov)
  • A shortage of E-cadherin protein impairs the interaction between E-cadherin and p120-catenin and affects craniofacial development, leading to the features of BCD syndrome. (medlineplus.gov)
  • 29 Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) ELISA Kits from 12 manufacturers are available on www.antibodies-online.com. (antibodies-online.com)
  • Identification of CDH1 germline missense mutations associated with functional inactivation of the E-cadherin protein in young gastric cancer probands. (cdc.gov)
  • Direct evidence of E-cadherin mutations triggering tumorigenesis has come from the finding of inactivating germline mutations of the gene (CDH1) in hereditary diffuse gastric cancer (HDGC). (cdc.gov)
  • cDNAs encoding either the wild-type protein or mutant forms of E-cadherin were stably transfected into CHO (Chinese hamster ovary) E-cadherin-negative cells. (cdc.gov)
  • In this study, we investigated the uterine changes of AJ components E-cadherin, beta-catenin, and alpha-catenin at their mRNA and protein levels, together with the cellular distribution of meprinbeta, phospho-beta-catenin, and active beta-catenin proteins, in hamsters that show only ovarian progesterone-dependent uterine receptivity and implantation. (bioone.org)
  • This gene encodes a large protein that contains many cadherin domains and likely functions in cell adhesion. (genecards.org)
  • DCHS2 (Dachsous Cadherin-Related 2) is a Protein Coding gene. (genecards.org)
  • The cytoplasmic domain of E-cadherin is reported to interact with a range of intracellular proteins including erbin, ezrin, caspase-3, caspase-8, β-catenin, presenilin 1, and casein kinase II. (stemcell.com)
  • E-cadherin expression is regulated at various levels including gene expression, protein stability, and intracellular protein distribution ( Halbleib and Nelson, 2006 ). (rupress.org)
  • Cadherin-1 (not to be confused with the APC/C activator protein CDH1) also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin is a protein that in humans is encoded by the CDH1 gene. (wikipedia.org)
  • Several proteins such as SNAI1/SNAIL, ZFHX1B/SIP1, SNAI2/SLUG, TWIST1 and DeltaEF1 have been found to downregulate E-cadherin expression. (wikipedia.org)
  • We investigated DNMT1, DNMT3b and E-Cadherin (CDH1) mRNA and protein expression levels in an in vitro model of Huh-7 cells expressing the HCV core protein of different genotypes: 1b, 2a, 3a, 4h and 5a. (springer.com)
  • Germline sequence analysis of CDH1 (encoding the adhesion protein cadherin-1), a known driver, revealed an essential splice-site mutation co-segregating within the pedigree. (biomedcentral.com)
  • CDH1 encodes E-cadherin, a glycoprotein that plays an important part in cell-cell interaction. (springer.com)
  • Loss of expression and aberrant methylation of the cdh1 (e-cadherin) gene in breast cancer patients from Kashmir. (springer.com)
  • Cdh1 polymorphisms and low expression of e-cadherin and beta-catenin in colorectal cancer patients. (springer.com)
  • Association between e-cadherin (cdh1) polymorphisms and pancreatic cancer risk in Han Chinese population. (springer.com)
  • 25 Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) ELISA Kits von 11 Herstellern verfügbar auf www.antikoerper-online.de. (antikoerper-online.de)
  • E-cadherin has many other functions including acting as a tumor suppressor protein, which means it prevents cells from growing and dividing too rapidly or in an uncontrolled way. (medlineplus.gov)
  • When both copies of the CDH1 gene are mutated in a particular cell, that cell cannot produce any functional E-cadherin. (medlineplus.gov)
  • Cell-cell adhesion is mediated by extracellular cadherin domains, whereas the intracellular cytoplasmic tail associates with numerous adaptors and signaling proteins, collectively referred as the cadherin adhesome. (wikipedia.org)
  • Each cadherin has a small C-terminal cytoplasmic component, a transmembrane component, and the remaining bulk of the protein is extra-cellular (outside the cell). (wikipedia.org)
  • Once the cell-cell adhesion between cadherins present in the cell membranes of two different cells has formed, adherens junctions can then be made when protein complexes, usually composed of α-, β-, and γ-catenins, bind to the cytoplasmic portion of the cadherin. (wikipedia.org)
  • An important gene associated with Sarcomatoid Transitional Cell Carcinoma is CDH1 (Cadherin 1). (malacards.org)
  • Recognizes a protein of 120-80kDa, identified as E-cadherin. (genetex.com)
  • The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function. (genetex.com)
  • Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with E-Cadherin Monoclonal Antibody (CDH1/1525). (genetex.com)
  • Western Blot Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525). (genetex.com)
  • CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. (uniprot.org)
  • This protein is found within the membrane that surrounds epithelial cells, which are the cells that line the surfaces and cavities of the body, such as the inside of the eyelids and mouth. (medlineplus.gov)
  • By combining logic-based network modeling, in vitro experimentation, and gene expression profiles from patient cohorts displaying tumor aggressiveness, we identify and experimentally validate distinctive, tumor type-specific signatures of receptor proteins associated to epithelial-mesenchymal transition in bladder and breast cancer. (nature.com)
  • By in situ hybridization and immunofluorescence, we have demonstrated that uterine epithelial cells expressed three of these AJ proteins and their mRNAs prior to and during the initial phase of implantation. (bioone.org)
  • Immunofluorescence study showed no change in epithelial expression patterns of uterine AJ proteins from Days 1 to 5 of pregnancy. (bioone.org)
  • We previously showed that the epithelial cell-type-specific proteins e pithelial s plicing r egulatory p roteins 1 (ESRP1) and ESRP2 are important for the regulation of many AS events that are altered during EMT. (asm.org)
  • Here, we applied proteomics, expression analysis and functional studies in primary murine ATII cells to identify proteins and molecular mechanisms involved in alveolar epithelial plasticity. (biologists.org)
  • Our data thus identified proteins involved in ATII-to-ATI cell trans-differentiation and suggest a Wnt/β-catenin-driven functional role of ENO1 and PDIA3 in alveolar epithelial cell plasticity in lung injury and repair. (biologists.org)
  • Cdc20 and Cdh1 activate APC/C by contributing to substrate recognition, among other mechanisms ( 1 ⇓ - 3 ). (pnas.org)
  • This proteolysis is mediated by the ubiquitin-proteasome system, with the E3 ligase being the anaphase-promoting complex, also known as the cyclosome (APC/C). The APC/C is regulated by two activators, namely Cdc20 and Cdh1. (biologists.org)
  • The APC/C is activated by two cofactors, Cdc20 and Cdh1. (biologists.org)
  • Here, we identify the conserved Ube2S as a K11-specific chain elongating E2 for human and Drosophila APC/C. Ube2S depends on the cell cycle-dependent association with the APC/C activators Cdc20 and Cdh1 for its activity. (pnas.org)
  • Cdc20 and Cdh1 recruit substrates to the APC/C ( 17 , 18 ), but also increase the catalytic activity of the APC/C by a poorly understood mechanism. (pnas.org)
  • However, the function of cell division cycle proteins that are also involved in this process, such as CDC20 and CDH1 is totally unknown. (le.ac.uk)
  • Definition of Cdh1 interactions with the APC/C indicates how they are antagonized by Cdh1 phosphorylation. (rcsb.org)
  • When cells enter mitosis, the APC/C is partially activated through phosphorylation of core subunits and binding of the WD40-repeat protein Cdc20 ( 9 , 13 , 14 ). (pnas.org)
  • Accordingly, inactivation of the APC/C before S phase involves phosphorylation and degradation of Cdh1 and binding of the Cdh1-inhibitor Emi1 ( 5 ). (pnas.org)
  • Cdh1 phosphorylation at Cdk sites promotes Cdh1 stability. (jneurosci.org)
  • A , Schematic of nine conserved sites of potential Cdk phosphorylation in Cdh1 (S/T-P), numbered according to human Cdh1. (jneurosci.org)
  • Cdh1 phosphorylation at Cdk sites disrupts the association of Cdh1 with the APC core protein Cdc27. (jneurosci.org)
  • Cdh1 phosphorylation at Cdk sites promotes the cytoplasmic accumulation of Cdh1 in granule neurons. (jneurosci.org)
  • Phosphorylation of Cdh1 inhibits its ability to limit axon growth. (jneurosci.org)
  • Among these checkpoint components are protein kinases and phosphoproteins suggesting that checkpoint function requires a phosphorylation-based transduction pathway. (rupress.org)
  • RT "A tissue-specific atlas of mouse protein phosphorylation and RT expression. (genome.jp)
  • The encoded protein catalyzes phosphorylation of phosphatidylinositol 4-phosphate, producing phosphatidylinositol 4,5-bisphosphate. (nih.gov)
  • On the molecular level, JNK influences cellular functions by tagging other proteins with a phosphate chemical group (a process known as phosphorylation), a common mechanism cells use to turn enzymes on and off. (healthcanal.com)
  • Changes in global protein phosphorylation patterns in the Δcdc20 mutant parasites were largely different from those observed in the Δmap2 mutant. (le.ac.uk)
  • Further studies showed that UA inhibited the phosphorylation of Akt and ERK proteins. (greenmedinfo.com)
  • [5] CDH1 has also been designated as CD324 ( cluster of differentiation 324). (wikipedia.org)
  • A cadherina-1 tamén chamada E-cadherina ou cadherina epitelial , ou tamén CAM 120/80 , uvomorulina ou CD324 ( cluster de diferenciación 324), é unha proteína que nos humanos está codificada no xene CDH1 do cromosoma 16 . (wikipedia.org)
  • E2F1 is a remarkable example of a network hub as this protein interacts with many genes, proteins, and other transcription factors through a variety of regulatory mechanisms. (nature.com)
  • Active repression of methylated genes by the chromosomal protein MBD1. (semanticscholar.org)
  • SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins. (semanticscholar.org)
  • Although only 2.94% of the genome was revealed to encode protein coding genes, subsequent large-scale transcriptomic analyses, such as deep sequencing and/or whole genome high-density tiling array analyses combined with bioinformatics, revealed that the majority of the genome (74.7%) was indeed transcribed but was not translated into proteins ( 2 , 3 ). (bioscience.org)
  • These different conformational changes inhibit specific RB-protein interactions. (nih.gov)
  • The protein-protein interactions are mapped onto this pathway. (wikipathways.org)
  • E2F1 has direct interactions with proteins and molecules. (creativebiomart.net)
  • The concept of protein cross-linking as a (bio)chemical tool to infer structural information about protein conformations and protein-protein interactions in combination with mass spectrometry was introduced at the end of the 1990s ( 1 ). (mcponline.org)
  • High throughput generation of data supporting protein fold prediction and the determination of protein-protein interactions have not been realized routinely. (mcponline.org)
  • We critically discuss advantages and limitations of different concepts and look beyond the immediate outcome of cross-linking experiments (putative interactions and/or distance constraints) and examine the potential role of chemical cross-linking in the analysis of protein interaction networks and, more generally, for structural and systems biology. (mcponline.org)
  • Representative image of Proximity Ligation Assay of protein-protein interactions between CASP3 and CTTN. (abnova.com)
  • An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma. (abnova.com)
  • Cdc20 (fizzy (fzy)) or p55CDC, and Cdh1 (Hct1, srw1 or fizzy-related 1 (fzr1)) encode two adaptor proteins that bring substrates to the APC. (nyu.edu)
  • Furthermore, D-box peptides diminish the ubiquitination of KEN-box substrates by APC/C Cdh1 , suggesting possible competition between the two motifs. (pnas.org)
  • Aurora A and B were the only substrates stabilised following Cdh1 RNAi. (biologists.org)
  • This review summarizes what we currently understand about how the action of septin-associated protein kinases and their substrates control information flow to drive the cell cycle into and out of mitosis, to regulate bud growth, and especially to direct timely and efficient execution of cytokinesis and cell abscission. (frontiersin.org)
  • Cdh1 protein is a limiting, substrate-specific activator of anaphase-promoting complex (APC)-dependent proteolysis and it is required for the degradation of the APC substrates, such as Clb2 and Ase1. (thermofisher.com)
  • Two major regulators are cell-division cycle protein 20 (CDC20) and its homologue, CDC20 homologue 1 (CDH1), which activate the anaphase-promoting complex/cyclosome (APC/C) in mitosis, and facilitate degradation of mitotic APC/C substrates. (le.ac.uk)
  • CDH1/FZR is an activator of the anaphase-promoting complex/cyclosome (APC/C activator), a ubiquitin E3 ligase that targets mitotic cyclins for degradation by the 26S proteasome. (genetics.org)
  • In anaphase, APC/C bound to the Cdc20 homolog Cdh1 (APC/C Cdh1 ) further mediate the ubiquitination of cyclin B1 and other mitotic regulators, resulting in their degradation and mitotic exit. (pnas.org)
  • The proteasomal degradation of proteins is essential for cell division in all eukaryotes. (pnas.org)
  • Based on this and other clues, Bonni identified a segment in the fragile X protein where Cdh1-APC binds and marks it for degradation. (eurekalert.org)
  • If we can find a way to block the interaction between Cdh1-APC and the fragile X mental retardation protein or to block the ability of Cdh1-APC to cause degradation of the protein, that should make more of the fragile X protein available in the brain and reduce some of the symptoms experienced by carriers of this disorder," he said. (eurekalert.org)
  • Promotes ubiquitination of tyrosine-phosphorylated CDH1, thus targeting CDH1 for endocytosis and degradation. (abcam.com)
  • To determine whether vasopressin-induced alterations in protein abundance result from modulation of protein production, protein degradation, or both, we used protein mass spectrometry with dynamic stable isotope labeling in cell culture to achieve a proteome-wide determination of protein half-lives and relative translation rates in mpkCCD cells. (asnjournals.org)
  • First, as a germline CDH1 mutation was found, it tells us that an early chapter in the story was loss of the wild-type allele of this gene, probably coinciding with biallelic loss of the TP53 gene (encoding cellular tumor antigen p53) or preceding it. (biomedcentral.com)
  • Interacts with the CDC2 protein kinase to form MPF. (string-db.org)
  • More surprisingly, by moderating the rate at which Cdk1 is activated following GVBD, APC/C Cdh1 creates conditions necessary for the removal of shugoshin-2 from chromosome arms by the Aurora B/C kinase, an event crucial for the efficient resolution of chiasmata. (nih.gov)
  • Schematic depiction of the F box protein S phase kinase-associated protein 2 (SKP2) recognizing phosphorylated p27. (nih.gov)
  • Proto-oncogene tyrosine-protein kinase Src , also known as proto-oncogene c-Src or simply c-Src , is a non-receptor tyrosine kinase protein that in humans is encoded by the SRC gene . (wikidoc.org)
  • This induces long-range allostery via protein domain dynamics , causing the structure to be destabilized, resulting in the opening up of the SH3, SH2 and kinase domains and the autophosphorylation of the residue tyrosine 416. (wikidoc.org)
  • For instance, the disruption of calcineurin, a Ca 2+ -dependent phosphatase, or Ca 2+ /calmodulin-dependent protein kinase II or IV, two Ca 2+ -dependent kinases, profoundly impairs β-cell function, likely by modulating the activity of Ca 2+ -responsive transcription factors such as NFAT, CREB, and TORC2 ( 5 - 9 ). (diabetesjournals.org)
  • A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. (abnova.com)
  • For each exemplified protein there is a short descriptive text including the clinical usefulness of the corresponding antibody. (proteinatlas.org)
  • This antibody is purified through a protein A column, followed by peptide affinity purification. (avivasysbio.com)
  • Cdh1 antibody detects Cdh1 protein on zebrafish by whole mount immunohistochemical analysis. (genetex.com)
  • Cdh1 antibody (GTX125890) dilution: 1:50. (genetex.com)
  • B , Lysates of 293T cells transfected with GFP-Cdh1 WT, 9A, or 9D were immunoblotted using a polyclonal antibody to Cdh1 or Erk, the latter to serve as a loading control. (jneurosci.org)
  • E , Lysates of Neuro2A cells or granule neurons treated with roscovitine (20 μ m ) or DMSO were immunoblotted using a monoclonal antibody to Cdh1 and the Erk or 14-3-3β antibody. (jneurosci.org)
  • F , Lysates of granule neurons treated with MG132 (10 μ m ), roscovitine (20 μ m ), both MG132 and roscovitine, or DMSO were immunoblotted using the Erk antibody or the Cdh1 monoclonal antibody. (jneurosci.org)
  • A , The APC core protein Cdc27 was immunoprecipitated (IP) from lysates of 293T cells expressing GFP-Cdh1 WT, 9A, or 9D followed by immunoblotting with the GFP or Cdc27 antibody. (jneurosci.org)
  • B , Glycerol density gradient (15-35%) centrifugation of extracts was performed on cerebellar granule neurons, and fractions were immunoblotted using a Cdc27 antibody and a monoclonal antibody to Cdh1. (jneurosci.org)
  • A , Granule neurons transfected with an expression plasmid encoding GFP-Cdh1 WT, 9D, or 9A, together with the β-gal expression plasmid were subjected to immunocytochemistry with an antibody to β-gal or GFP and the DNA dye Hoechst. (jneurosci.org)
  • A , Granule neurons transfected with an expression plasmid encoding GFP-Cdh1-Res, GFP-Cdh1-Res 9A, GFP-Cdh1-Res 9D, or the control vector, together with the U6/Cdh1 RNAi plasmid and DsRed expression plasmid were subjected to immunocytochemistry using a DsRed antibody. (jneurosci.org)
  • This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the CASP3 protein, and the other against the CTTN protein for use in in situ Proximity Ligation Assay . (abnova.com)
  • Both APC-Cdc20 and APC-Cdh1 have been implicated in the control of mitosis through mediating ubiquitination of mitotic regulators, such as cyclin B1 and securin. (nyu.edu)
  • The completion of mitosis depends on protein ubiquitination by the anaphase-promoting complex (APC). (nih.gov)
  • We conclude that ubiquitination of a Cdh1 inhibitor by APC(Cdc20) helps establish the order of activation of the two APC isoforms. (nih.gov)
  • Although both motifs contribute to securin ubiquitination by APC/C Cdh1 , securin mutants lacking either motif are efficiently ubiquitinated. (pnas.org)
  • Figure 3: pRB-APCcdh1 association, ubiquitination of Skp2 and p27Kip1 protein accumulation are coordinated during pRB-mediated cell-cycle arrest. (nature.com)
  • protein ubiquitination. (abcam.com)
  • CCS52A1 encodes a CDH1/FZR-like protein, a class of proteins that function as activators of the anaphase-promoting complex. (genetics.org)
  • A gene on chromosome 17q21.2 that encodes the smallest of all type-I keratin proteins, which is expressed in the periderm, the transiently superficial layer that surrounds the developing epidermis. (thefreedictionary.com)
  • a gene encodes information not only for protein-coding RNAs but also for ncRNAs. (bioscience.org)
  • This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. (abnova.com)
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)
  • The bar plot below shows the proportion of tumor samples that have any kind of altering mutation(s) in the given protein. (phosphosite.org)
  • An additional mutation that impairs the normal copy of the CDH1 gene is needed for cancer to develop. (medlineplus.gov)
  • The 9D mutation, but not the 9A mutation, disrupts the ability of Cdh1 to form a complex with the APC core protein Cdc27. (jneurosci.org)
  • Thus, mutation of CDH1 and TP53 are early events that probably drove tumor initiation and set the ball rolling. (biomedcentral.com)
  • It is likely that 20 to 40 percent of individuals with HDGC have a mutation in the CDH1 gene. (medlineplus.gov)
  • About 60 to 70 percent of individuals with HDGC do not have an identified mutation in the CDH1 gene. (medlineplus.gov)
  • In contrast, carriers of the mutation make the protein but produce significantly less of it than people without the mutation. (eurekalert.org)
  • Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. (string-db.org)
  • The current view is that prior to anaphase, the APC/C is activated by Cdc20, but that following anaphase, APC/C switches to Cdh1-dependent activation. (biologists.org)
  • However, once the spindle checkpoint has become satisfied and anaphase initiated, APC/C activation switches from Cdc20 to Cdh1 ( Peters, 2006 ). (biologists.org)
  • Soon after anaphase onset, Cdc20 is degraded and replaced by Cdh1, which maintains APC/C activity during late mitosis and early G1 ( 15 , 16 ). (pnas.org)
  • The MA5-11496 immunogen is recombinant human cdh1 protein. (thermofisher.com)
  • Recombinant Human CDH1 precusor extracellular domain (NP_004351.1) (Met 1-Ile 707), fused with a C-terminal polyhistidine tag, was produced in Human Cell. (creativebiomart.net)
  • We found that both mRNA and protein expression levels of DNMT1 and 3b were upregulated in genotype 1b HCV core expressing cells as compared to control cells. (springer.com)
  • DNMT3b mRNA levels did not change in genotypes 2a, 3a, 4h and 5a, but were upregulated at the protein level by genotype 1b, 2a, 3a. (springer.com)
  • CDH1 mRNA expression was downregulated only in genotype 1b, whereas its protein expression resulted in downregulation by the HCV core of genotypes 1b, 2a and 3a. (springer.com)
  • Recent studies in a cultured mouse cell line called "mpkCCD" established that vasopressin regulates the abundances of many proteins in collecting duct cells in the absence of altered mRNA abundances. (asnjournals.org)
  • Endoreplication requires downregulation of mitotic A-type and B-type cyclins, as well as the Drosophila CDC25 homolog String and other factors that promote mitosis, coupled with the upregulation of CDH1/FZR. (genetics.org)
  • The APC is activated by association with Cdc20 in midmitosis and Cdh1 in late mitosis and G1. (nih.gov)
  • Later in mitosis, Acm1 destruction is also promoted by APC(Cdh1). (nih.gov)
  • Thus, a normal role for Cyclin A/Cdk2 during early G2 phase is to increase the level of Cdh1 which destabilises Claspin which in turn down regulates Chk1 activation to allow progression into mitosis. (biomedsearch.com)
  • This mechanism links S phase exit with G2 phase transit into mitosis, provides a novel insight into the roles of Cyclin A/Cdk2 in G2 phase progression, and identifies a novel role for APC/C(Cdh1) in late S/G2 phase cell cycle progression. (biomedsearch.com)
  • We also speculate that the ability of APC(Cdh1) to target its own inhibitor enhances the bistability of the late mitotic regulatory system. (nih.gov)
  • Here we overcome these limitations by generating an in vivo reporter system using the scaffolding protein anillin fused to enhanced green fluorescent protein, to provide high spatiotemporal resolution of mitotic phase. (nih.gov)
  • The mitotic APC/C activator, the cell division cycle 20 (Cdc20) protein, directly interacts with APC/C degrons--the destruction (D) and KEN boxes. (pnas.org)
  • It has been shown that human CKAP2 is degraded by APC/C-Cdh1 during mitotic exit and that a tight regulation of CKAP2 protein level is important for mitotic progression [ PMID: 17376772 ]. (ebi.ac.uk)
  • CKAP2 is a spindle-associated protein degraded by APC/C-Cdh1 during mitotic exit. (ebi.ac.uk)
  • Cdh1 plays important role in mitotic regulation. (thermofisher.com)
  • Insc and Lgn drive the orientation of the spindle by capturing cortical microtubules via nuclear mitotic apparatus protein 1 (Numa1) ( Poulson and Lechler, 2010 ). (biologists.org)
  • More than 120 inherited mutations in the CDH1 gene have been found to cause a familial cancer disorder called hereditary diffuse gastric cancer (HDGC). (medlineplus.gov)
  • Inherited inactivating mutations in CDH1 are associated with Hereditary Diffuse Gastric Cancer. (wikipedia.org)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • We find that Acm1 uses pseudosubstrate and other sequence motifs to bind and inhibit Cdh1, but not Cdc20. (nih.gov)
  • p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence. (uniprot.org)
  • section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. (uniprot.org)
  • Please note the EGFP protein sequence in this plasmid is missing the first two amino acids of the standard EGFP sequence (Met-Val) due to the cloning strategy. (addgene.org)
  • The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. (abnova.com)
  • One function of these structures that has been well-documented in studies conducted in budding yeast Saccharomyces cerevisiae is to serve as a scaffold that recruits regulatory proteins, which dictate the spatial and temporal control of certain aspects of the cell division cycle. (frontiersin.org)
  • The intracellular portion of classical cadherins has regulatory proteins that helps the cadherins joining the actin cytoskeleton. (wikipedia.org)
  • Regulatory proteins include p-120 catenin, α {\displaystyle \alpha } -catenin, β {\displaystyle \beta } -catenin, and vinculin. (wikipedia.org)
  • Cell-cycle progression is governed by a series of essential regulatory proteins. (le.ac.uk)
  • Vasopressin regulates water excretion, in part, by controlling the abundances of the water channel aquaporin-2 (AQP2) protein and regulatory proteins in the renal collecting duct. (asnjournals.org)
  • Inactivation of CDH1 in 50% of diffuse gastric carcinomas. (wikipedia.org)
  • People with CDH1 gene mutations associated with HDGC have a 56 to 70 percent chance of developing stomach (gastric) cancer in their lifetimes. (medlineplus.gov)
  • We screened a series of 66 young gastric cancer probands for germline CDH1 mutations, and two novel missense alterations together with an intronic variant were identified. (cdc.gov)
  • Individuals with this condition have up to a 70% lifetime risk of developing diffuse gastric carcinoma, and females with CDH1 mutations have up to a 60% lifetime risk of developing lobular breast cancer. (wikipedia.org)
  • Deregulation of arid1a, cdh1, cmet and pik3ca and target-related microrna expression in gastric cancer. (springer.com)
  • Up-regulation of cytoskeletal-associated protein 2 in primary human gastric adenocarcinomas. (ebi.ac.uk)
  • A lack of this protein, which is critical for cell adhesion, may also make it easier for cancer cells to detach from a primary tumor and spread (metastasize) to other parts of the body. (medlineplus.gov)
  • The RNA sample with the higher integrity (C71A) recorded lower Cqs for KRT19 (keratin 19), UBC#1, and HMBS (hydroxymethylbilane synthase), but not for TP53I3 (tumor protein p53 inducible protein 3) and GAPDH (see online Supplemental Tab 12). (thefreedictionary.com)
  • TP53I3, tumor protein p53 inducible protein 3. (thefreedictionary.com)
  • The loss of this protein prevents it from acting as a tumor suppressor, contributing to the uncontrollable growth and division of cells . (medlineplus.gov)
  • Inherited mutations in the CDH1 gene increase a woman's risk of developing a form of breast cancer that begins in the milk-producing glands (lobular breast cancer). (medlineplus.gov)
  • Inherited mutations in the CDH1 gene are thought to account for only a small fraction of all breast cancer cases. (medlineplus.gov)
  • CDH1 gene mutations also occur commonly in lobular breast cancers in women without a family history of the disease. (medlineplus.gov)
  • People with HDGC caused by CDH1 gene mutations are born with one mutated copy of the gene in each cell. (medlineplus.gov)
  • At least five inherited CDH1 gene mutations have been identified in people with blepharocheilodontic (BCD) syndrome. (medlineplus.gov)
  • Despite the association of CDH1 gene mutations with increased cancer risk (see below), it is unclear whether people with BCD syndrome are at increased risk of developing cancer. (medlineplus.gov)
  • p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors. (uniprot.org)
  • In addition to gross alterations in spindle structure, the budding yeast spindle checkpoint can also respond to low doses of microtubule depolymerizing drugs, to defects induced by mutations in centromere-binding proteins or in centromeric DNA, and to aberrantly segregating centromeres (for review see Rudner and Murray, 1996 ). (rupress.org)
  • These CDH1 gene mutations also lead to a 40 to 50 percent chance of lobular breast cancer in women, a slightly increased risk of prostate cancer in men, and a slightly increased risk of colorectal cancer. (medlineplus.gov)
  • It is unclear why CDH1 gene mutations primarily occur in the stomach lining and these other tissues. (medlineplus.gov)
  • Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed:11976333, PubMed:16787920). (genecards.org)
  • RT "The PDZ protein tax-interacting protein-1 inhibits beta-catenin RT transcriptional activity and growth of colorectal cancer cells. (genome.jp)
  • These results indicate that UA inhibits cell proliferation and induces apoptosis in colon cancer cells through simultaneous modulationof the multiple signaling pathways such as MMP9/CDH1, Akt/ERK, COX-2/PGE2, p300/NF-κB/CREB2, and cytochrome c/caspase pathways. (greenmedinfo.com)
  • MA5-11496 targets APC/C activator protein CDH1 in IP, ICC/IF and WB applications and shows reactivity with Human samples. (thermofisher.com)
  • Here we show that depletion of Cyclin A or inhibition of Cdk2 during late S/early G2 phase maintains the G2 phase arrest by reducing Cdh1 transcript and protein levels, thereby stabilizing Claspin and maintaining elevated levels of activated Chk1 which contributes to the G2 phase observed. (biomedsearch.com)
  • Two transcript variants encoding the same protein have been found for this gene. (wikidoc.org)
  • Alternative splicing of this gene results in two transcript variants that encode the same protein. (abnova.com)
  • Vezatin, a protein associated to adherens junctions, is required for mouse blastocyst morphogenesis. (xenbase.org)
  • Regulates surface levels of adherens junction proteins such as CDH1 . (rcsb.org)
  • Modulates adherens junctions formation by facilitating CDH1 trafficking. (nih.gov)
  • Although these two observations are consistent with Cenp-F being a substrate of Cdh1-activated APC/C, Cenp-F is degraded normally in Cdh1-null cells. (biologists.org)
  • The hepatitis C virus core protein of genotypes 3a and 1b downregulates insulin receptor substrate 1 through genotype-specific mechanisms. (springer.com)
  • The adaptor proteins that associate with desmosomal cadherins are plakoglobin (related to β {\displaystyle \beta } -catenin), plakophilins (p120 catenin subfamily), and desmoplakins. (wikipedia.org)
  • Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2). (nih.gov)
  • Among its related pathways are G-protein signaling_RhoA regulation pathway and Signaling by GPCR . (genecards.org)
  • Budding yeast ( S. cerevisiae ) has served as a path-finding model eukaryote in which to explore the structure, function, and regulation of septins and septin-associated proteins. (frontiersin.org)
  • We describe a previously unknown mode of regulation of DNMT1 protein stability through the coordinated action of an array of DNMT1-associated proteins. (sciencemag.org)
  • When any of these layers of protein regulation fail, cell growth can get out of hand. (healthcanal.com)
  • Cell-cycle regulation and DNA replication in Plasmodium was recently shown to be dependent on the activity of a number of protein kinases. (le.ac.uk)
  • In particular, processes that are rapidly regulated, such as transcription, endocytosis, cell cycle regulation, and ubiquitylation are associated with proteins with especially short half-lives. (asnjournals.org)
  • 1 ) regulated trafficking, 1 seen in a time frame of 40 seconds to about 40 minutes, 2 and ( 2 ) regulation of AQP2 protein abundance, typically seen after many hours of exposure to vasopressin. (asnjournals.org)
  • 13 This finding implies that post-transcriptional regulation of protein abundances in response to vasopressin in collecting duct cells is not unique to AQP2. (asnjournals.org)
  • These proteins were then used to determine the most important pathways involved in Breast Cancer by using the Human Pathway Database (HPD). (wikipathways.org)
  • The pathways retrieved from the Human Pathway Database were from several sources such as Protein Lounge, BioCarta, KEGG, and NCI-Nature. (wikipathways.org)
  • Protein-protein relations for the most important proteins for Breast Cancer were determined by annotating the pathways and by literature review. (wikipathways.org)
  • In particular, as discussed here, septin-based structures recruit, and thereby localize (and, in some cases, regulate the activity of) a multiplicity of protein kinases that integrate multiple inputs into signaling pathways and ultimately initiate ensuing biological responses (Figure 1 ). (frontiersin.org)
  • We selected most pathways E2F1 participated on our site, such as Activation of BH3-only proteins, Activation of NOXA and translocation to mitochondria, Activation of PUMA and translocation to mitochondria, which may be useful for your reference. (creativebiomart.net)
  • This pathway incorporates the most important proteins for Breast Cancer. (wikipathways.org)
  • Analysis of its overexpression in mad , bub , and mps1 mutants suggests that the Bub1 and Mps1 kinases act together at an early step in the checkpoint pathway, upstream of all the other Bub and Mad proteins ( Farr and Hoyt, 1998 ). (rupress.org)
  • A deficiency in ACADVL protein reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy.The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. (avivasysbio.com)
  • Also, other proteins which involved in the same pathway with E2F1 were listed below. (creativebiomart.net)
  • Model of active and complexes with E2F and an 'L-X-C-X-E' peptide (Protein Data Bank (PDB) codes: 2QDJ, 1GUX, 1N4M and 2AZE). (nih.gov)
  • Chemical cross-linking of reactive groups in native proteins and protein complexes in combination with the identification of cross-linked sites by mass spectrometry has been in use for more than a decade. (mcponline.org)
  • Recent advances in instrumentation, cross-linking protocols, and analysis software have led to a renewed interest in this technique, which promises to provide important information about native protein structure and the topology of protein complexes. (mcponline.org)
  • In contrast, we noted strong expression of beta-catenin and alpha-catenin proteins in the PDZ, but not in the SDZ, of mice. (bioone.org)
  • RT "AlphaT-catenin: a novel tissue-specific beta-catenin-binding protein RT mediating strong cell-cell adhesion. (genome.jp)
  • In particular, septin-associated protein kinases couple cell cycle progression with cellular morphogenesis. (frontiersin.org)
  • In this paper, we demonstrate that the inhibition of mitochondrial fission protein Drp1 causes an unexpected delay in G2/M cell cycle progression and aneuploidy. (biologists.org)
  • However, how deficiencies in the proteins that regulate mitochondrial dynamics impact cell cycle progression and hence directly contribute to the development of diseases is not clear. (biologists.org)
  • Biochemical and biophysical studies have established that Cdh1 and the APC/C subunit Apc10 serve as coreceptors for the D box, with the D box bridging an interaction between the two ( 7 ⇓ ⇓ ⇓ - 11 ). (pnas.org)
  • Tellinghuisen TL, Rice CM. Interaction between hepatitis C virus proteins and host cell factors. (springer.com)
  • Protein protein interaction immunofluorescence result. (abnova.com)
  • Each red dot represents the detection of protein-protein interaction complex. (abnova.com)
  • Cyclin A/Cdk2 regulates Cdh1 and claspin during late S/G2 phase of the cell cycle. (biomedsearch.com)
  • GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. (rcsb.org)
  • GNA12-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). (genecards.org)
  • GNA12-dependent Rho signaling also regulates protein phosphatese 2A activation causing dephosphorylation of its target proteins (PubMed:15525651, PubMed:17565996). (genecards.org)
  • These findings, which appeared online in Nature Cell Biology on June 27, showed that JNK, a protein already well known for other duties, also regulates the cell cycle. (healthcanal.com)
  • Antibodies have been selected to demonstrate the expression patterns of well-known proteins and to reflect antibodies used in clinical diagnostics to determine the nature of a given cancer. (proteinatlas.org)
  • For certain antibodies the corresponding protein expression pattern is shown in both normal and cancer tissues. (proteinatlas.org)
  • c ) pRB-APC cdh1 association was monitored over time by immunoprecipitation using anti-pRB antibodies. (nature.com)
  • The ectodomain of this protein mediates bacterial adhesion to mammalian cells, and the cytoplasmic domain is required for internalization. (wikipedia.org)
  • In contrast, 9A Cdh1 and 9D Cdh1 remained predominantly nuclear or nuclear and cytoplasmic, respectively, upon p25/Cdk5 expression. (jneurosci.org)
  • Normal and cancer tissues have been stained using immunohistochemistry to visualize various protein expression patterns. (proteinatlas.org)
  • High-resolution microscopy in embryonic heart and brain tissues of enhanced green fluorescent protein-anillin transgenic mice allows live monitoring of cell division and quantitation of cell cycle kinetics. (nih.gov)
  • In this study, CDH1 methylation and expression profile as well as prognosis of 38 cases of eyelid SCC and the corresponding adjacent tissues were analyzed to clarify the role of CDH1 methylation in SCC carcinogenesis and prognosis. (springer.com)
  • Interestingly, the Cyclin A/Cdk2 regulated APC/C(Cdh1) activity is selective for only a subset of Cdh1 targets including Claspin. (biomedsearch.com)
  • adhesion receptors , receptor tyrosine kinases , G-protein coupled receptors and cytokine receptors . (wikidoc.org)
  • In addition, and importantly, the activities of certain septin-associated protein kinases also regulate the state of organization of the septins themselves, creating a complex feedback loop. (frontiersin.org)
  • When CC polyubiquitin is free (unanchored-polyubiquitin), it also has CC distinct roles, such as in activation of protein kinases, and in CC signaling (By similarity). (univ-lyon1.fr)
  • Cadherins are a class of type-1 transmembrane proteins, and they are dependent on calcium (Ca2+) ions to function, hence their name. (wikipedia.org)
  • The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. (abnova.com)
  • 5A31: Structure of the human APC-Cdh1-Hsl1-UbcH10 complex. (rcsb.org)
  • EMDB-0601: Human ribosome nascent chain complex (CDH1-RNC) stalled by a drug. (pdbj.org)
  • Heterogeneity in the modification and involvement of chromatin components of the CpG island of the silenced human CDH1 gene in cancer cells. (semanticscholar.org)
  • These unexpected findings, that a much larger part of the human genome than was previously assumed is pervasively transcribed into ncRNA (non-protein-coding RNA), revolutionized our view of genome organization and content. (bioscience.org)
  • Deficiencies in the proteins regulating mitochondrial dynamics are associated with a number of human pathologies including neurodegenerative diseases and newborn lethality ( Westermann, 2010 ). (biologists.org)
  • Human FGFR1 full-length ORF ( NP_075594.1, 1 a.a. - 731 a.a.) recombinant protein with GST-tag at N-terminal. (abnova.com)
  • Cadherins are calcium-dependent cell adhesion proteins. (uniprot.org)
  • This protein is essential for the completion of the start, the controlling event, in the cell cycle. (string-db.org)
  • Thus, the enhanced green fluorescent protein-anillin system enables monitoring and measurement of cell division in vivo and markedly simplifies in vitro analysis in fixed cells. (nih.gov)
  • a ) Schemes of subcellular localization of the eGFP-anillin fusion protein during the cell cycle and of the CAG-eGFP-anillin fusion construct: Anillin is fused to the C terminus of eGFP and its expression is under control of the CAG promoter. (nih.gov)
  • In the G1 phase of the cell cycle, RB is phosphorylated at low levels and associates with E2F transcription factors, which are dimeric proteins containing E2F and differentiation-related polypeptide (DP) subunits. (nih.gov)
  • a ) pRB was induced by tetracycline in a stably transfected SAOS2 cell line (SAOS-tetRB) and protein samples for immunoblotting were harvested at various times after pRB induction. (nature.com)
  • Taken together, these results suggest that AJ proteins contribute to uterine barrier functions by cell-cell adhesion to ensure protection of the embryo. (bioone.org)
  • Conversely, the constitutive activation of calcineurin or calmodulin, a Ca 2+ binding protein, also causes marked β-cell dysfunction ( 3 , 10 , 11 ). (diabetesjournals.org)
  • Cadherins are synthesized as polypeptides and undergo many post-translational modifications to become the proteins which mediate cell-cell adhesion and recognition. (wikipedia.org)
  • FUNCTION: Stress-responsive protein involved in hormone responses, CC cell growth, and differentiation. (genome.jp)
  • To assure that the cell cycle - the cell's process of duplicating itself to make more cells - goes smoothly, a large network of proteins tells other proteins what to do and when to do it. (healthcanal.com)
  • Interplay between Cdh1 and JNK activity during the cell cycle. (healthcanal.com)
  • We've shown that Cdh1-APC's ability to do this is important to the development and structure of brain cell networks, but we also are interested in how it affects synapses, or the connections where nerve cells communicate with each other. (eurekalert.org)
  • Mass spectrometry of cultured ATII cells revealed a reduction of carbonyl reductase 2 (CBR2) and an increase in enolase 1 (ENO1) and protein disulfide-isomerase associated 3 (PDIA3) protein expression during ATII-to-ATI cell trans-differentiation. (biologists.org)
  • an ATI cell marker), exhibited decreased protein expression upon pharmacological and molecular Wnt/β-catenin inhibition in cultured ATII cells, whereas CBR2 levels were stabilized. (biologists.org)
  • UA also suppressed colon cancer cell migration by inhibiting MMP9 and upregulating CDH1 expression. (greenmedinfo.com)
  • The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. (abnova.com)
  • Abcg2 localizes to the nucleus and modulates cdh1 expression in lung cancer cells. (springer.com)