Basophils: Granular leukocytes characterized by a relatively pale-staining, lobate nucleus and cytoplasm containing coarse dark-staining granules of variable size and stainable by basic dyes.Basophil Degranulation Test: An in vitro test used in the diagnosis of allergies including drug hypersensitivity. The allergen is added to the patient's white blood cells and the subsequent histamine release is measured.Histamine Release: The secretion of histamine from mast cell and basophil granules by exocytosis. This can be initiated by a number of factors, all of which involve binding of IgE, cross-linked by antigen, to the mast cell or basophil's Fc receptors. Once released, histamine binds to a number of different target cell receptors and exerts a wide variety of effects.Antigens, CD8: Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.Antigens, CD3: Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).CD40 Ligand: A membrane glycoprotein and differentiation antigen expressed on the surface of T-cells that binds to CD40 ANTIGENS on B-LYMPHOCYTES and induces their proliferation. Mutation of the gene for CD40 ligand is a cause of HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 1.Antigens, CD40: A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.Antigens, CD34: Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.Antigens, CD44: Acidic sulfated integral membrane glycoproteins expressed in several alternatively spliced and variable glycosylated forms on a wide variety of cell types including mature T-cells, B-cells, medullary thymocytes, granulocytes, macrophages, erythrocytes, and fibroblasts. CD44 antigens are the principle cell surface receptors for hyaluronate and this interaction mediates binding of lymphocytes to high endothelial venules. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)Antigens, CD28: Costimulatory T-LYMPHOCYTE receptors that have specificity for CD80 ANTIGEN and CD86 ANTIGEN. Activation of this receptor results in increased T-cell proliferation, cytokine production and promotion of T-cell survival.Immunoglobulin E: An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).CD4-CD8 Ratio: Ratio of T-LYMPHOCYTES that express the CD4 ANTIGEN to those that express the CD8 ANTIGEN. This value is commonly assessed in the diagnosis and staging of diseases affecting the IMMUNE SYSTEM including HIV INFECTIONS.Antigens, CD63: Ubiquitously-expressed tetraspanin proteins that are found in late ENDOSOMES and LYSOSOMES and have been implicated in intracellular transport of proteins.Antigens, CD14: Glycolipid-anchored membrane glycoproteins expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein.Antigens, CD38: A bifunctional enzyme that catalyzes the synthesis and HYDROLYSIS of CYCLIC ADP-RIBOSE (cADPR) from NAD+ to ADP-RIBOSE. It is a cell surface molecule which is predominantly expressed on LYMPHOID CELLS and MYELOID CELLS.CD4-Positive T-Lymphocytes: A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.Antigens, CD: Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.Antigens, CD1: Glycoproteins expressed on cortical thymocytes and on some dendritic cells and B-cells. Their structure is similar to that of MHC Class I and their function has been postulated as similar also. CD1 antigens are highly specific markers for human LANGERHANS CELLS.Antigens, CD80: A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CTLA-4 ANTIGEN with high specificity and to CD28 ANTIGEN with low specificity. The interaction of CD80 with CD28 ANTIGEN provides a costimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.Receptors, IgE: Specific molecular sites on the surface of B- and T-lymphocytes which combine with IgEs. Two subclasses exist: low affinity receptors (Fc epsilon RII) and high affinity receptors (Fc epsilon RI).Antigens, CD2: Glycoprotein members of the immunoglobulin superfamily which participate in T-cell adhesion and activation. They are expressed on most peripheral T-lymphocytes, natural killer cells, and thymocytes, and function as co-receptors or accessory molecules in the T-cell receptor complex.Antigens, CD19: Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.Antigens, CD86: A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CD28 ANTIGEN with high specificity and to CTLA-4 ANTIGEN with low specificity. The interaction of CD86 with CD28 ANTIGEN provides a stimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.Antigens, CD18: Cell-surface glycoprotein beta-chains that are non-covalently linked to specific alpha-chains of the CD11 family of leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE-ADHESION). A defect in the gene encoding CD18 causes LEUKOCYTE-ADHESION DEFICIENCY SYNDROME.Antigens, CD95: A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.Antigens, CD5: Glycoproteins expressed on all mature T-cells, thymocytes, and a subset of mature B-cells. Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. The B-cell-specific molecule CD72 is a natural ligand for CD5. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)Antigens, CD56: The 140 kDa isoform of NCAM (neural cell adhesion molecule) containing a transmembrane domain and short cytoplasmic tail. It is expressed by all lymphocytes mediating non-MHC restricted cytotoxicity and is present on some neural tissues and tumors.Hypersensitivity: Altered reactivity to an antigen, which can result in pathologic reactions upon subsequent exposure to that particular antigen.Antigens, CD30: A member of the tumor necrosis factor receptor superfamily that may play a role in the regulation of NF-KAPPA B and APOPTOSIS. They are found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; NEUTROPHILS; EOSINOPHILS; MAST CELLS and NK CELLS. Overexpression of CD30 antigen in hematopoietic malignancies make the antigen clinically useful as a biological tumor marker. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.Mast Cells: Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.Antigens, CD24: A cell adhesion protein that was originally identified as a heat stable antigen in mice. It is involved in METASTASIS and is highly expressed in many NEOPLASMS.Antigens, CD36: Leukocyte differentiation antigens and major platelet membrane glycoproteins present on MONOCYTES; ENDOTHELIAL CELLS; PLATELETS; and mammary EPITHELIAL CELLS. They play major roles in CELL ADHESION; SIGNAL TRANSDUCTION; and regulation of angiogenesis. CD36 is a receptor for THROMBOSPONDINS and can act as a scavenger receptor that recognizes and transports oxidized LIPOPROTEINS and FATTY ACIDS.Antigens, CD9: A subtype of tetraspanin proteins that play a role in cell adhesion, cell motility, and tumor metastasis. CD9 antigens take part in the process of platelet activation and aggregation, the formation of paranodal junctions in neuronal tissue, and the fusion of sperm with egg.Interleukin-3: A multilineage cell growth factor secreted by LYMPHOCYTES; EPITHELIAL CELLS; and ASTROCYTES which stimulates clonal proliferation and differentiation of various types of blood and tissue cells.Antigens, CD20: Unglycosylated phosphoproteins expressed only on B-cells. They are regulators of transmembrane Ca2+ conductance and thought to play a role in B-cell activation and proliferation.Antigens, CD7: Differentiation antigens expressed on pluripotential hematopoietic cells, most human thymocytes, and a major subset of peripheral blood T-lymphocytes. They have been implicated in integrin-mediated cellular adhesion and as signalling receptors on T-cells.Antigens, CD43: A sialic acid-rich protein and an integral cell membrane mucin. It plays an important role in activation of T-LYMPHOCYTES.CD8-Positive T-Lymphocytes: A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.Antigens, CD11b: A CD antigen that contains a conserved I domain which is involved in ligand binding. When combined with CD18 the two subunits form MACROPHAGE-1 ANTIGEN.Antigens, CD11: A group of three different alpha chains (CD11a, CD11b, CD11c) that are associated with an invariant CD18 beta chain (ANTIGENS, CD18). The three resulting leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE ADHESION) are LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1; MACROPHAGE-1 ANTIGEN; and ANTIGEN, P150,95.Antigens, CD47: A ubiquitously expressed membrane glycoprotein. It interacts with a variety of INTEGRINS and mediates responses to EXTRACELLULAR MATRIX PROTEINS.Antigens, CD59: Small glycoproteins found on both hematopoietic and non-hematopoietic cells. CD59 restricts the cytolytic activity of homologous complement by binding to C8 and C9 and blocking the assembly of the membrane attack complex. (From Barclay et al., The Leukocyte Antigen FactsBook, 1993, p234)Antigens, CD70: A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds to CD27 ANTIGEN. It is found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; and DENDRITIC CELLS where it plays a role in stimulating the proliferation of CD4-POSITIVE T-LYMPHOCYTES and CD8-POSITIVE T-LYMPHOCYTES.Antibodies, Anti-Idiotypic: Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.Antigens, CD46: A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.Interleukin-4: A soluble factor produced by activated T-LYMPHOCYTES that induces the expression of MHC CLASS II GENES and FC RECEPTORS on B-LYMPHOCYTES and causes their proliferation and differentiation. It also acts on T-lymphocytes, MAST CELLS, and several other hematopoietic lineage cells.CD4 Lymphocyte Count: The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.Antigens, CD57: Oligosaccharide antigenic determinants found principally on NK cells and T-cells. Their role in the immune response is poorly understood.Anaphylaxis: An acute hypersensitivity reaction due to exposure to a previously encountered ANTIGEN. The reaction may include rapidly progressing URTICARIA, respiratory distress, vascular collapse, systemic SHOCK, and death.Eosinophils: Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.Allergens: Antigen-type substances that produce immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).Antigens, CD81: Tetraspanin proteins that are involved in a variety of cellular functions including BASEMENT MEMBRANE assembly, and in the formation of a molecular complexes on the surface of LYMPHOCYTES.Histamine: An amine derived by enzymatic decarboxylation of HISTIDINE. It is a powerful stimulant of gastric secretion, a constrictor of bronchial smooth muscle, a vasodilator, and also a centrally acting neurotransmitter.Leukotriene C4: The conjugation product of LEUKOTRIENE A4 and glutathione. It is the major arachidonic acid metabolite in macrophages and human mast cells as well as in antigen-sensitized lung tissue. It stimulates mucus secretion in the lung, and produces contractions of nonvascular and some VASCULAR SMOOTH MUSCLE. (From Dictionary of Prostaglandins and Related Compounds, 1990)Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Antigens, CD137: A member of the tumor necrosis factor receptor superfamily that is specific for 4-1BB LIGAND. It is found in a variety of immune cell types including activated T-LYMPHOCYTES; NATURAL KILLER CELLS; and DENDRITIC CELLS. Activation of the receptor on T-LYMPHOCYTES plays a role in their expansion, production of cytokines and survival. Signaling by the activated receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.CD30 Ligand: A membrane-bound tumor necrosis family member found primarily on activated T-LYMPHOCYTES that binds specifically to CD30 ANTIGEN. It may play a role in INFLAMMATION and immune regulation.Antigens, CD31: Cell adhesion molecules present on virtually all monocytes, platelets, and granulocytes. CD31 is highly expressed on endothelial cells and concentrated at the junctions between them.Antigens, CD11c: An integrin alpha subunit of approximately 150-kDa molecular weight. It is expressed at high levels on monocytes and combines with CD18 ANTIGEN to form the cell surface receptor INTEGRIN ALPHAXBETA2. The subunit contains a conserved I-domain which is characteristic of several of alpha integrins.Antigens, CD151: Tetraspanin proteins found associated with LAMININ-binding INTEGRINS. The CD151 antigens may play a role in the regulation of CELL MOTILITY.Antigens, CD55: GPI-linked membrane proteins broadly distributed among hematopoietic and non-hematopoietic cells. CD55 prevents the assembly of C3 CONVERTASE or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex.Strongylida Infections: Infections with nematodes of the order STRONGYLIDA.Th2 Cells: Subset of helper-inducer T-lymphocytes which synthesize and secrete the interleukins IL-4, IL-5, IL-6, and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.Cell Degranulation: The process of losing secretory granules (SECRETORY VESICLES). This occurs, for example, in mast cells, basophils, neutrophils, eosinophils, and platelets when secretory products are released from the granules by EXOCYTOSIS.Desensitization, Immunologic: Immunosuppression by the administration of increasing doses of antigen. Though the exact mechanism is not clear, the therapy results in an increase in serum levels of allergen-specific IMMUNOGLOBULIN G, suppression of specific IgE, and an increase in suppressor T-cell activity.Nippostrongylus: A genus of intestinal nematode parasites belonging to the superfamily HELIGMOSOMATOIDEA, which commonly occurs in rats but has been experimentally transmitted to other rodents and rabbits. Infection is usually through the skin.Antigens, CD58: Glycoproteins with a wide distribution on hematopoietic and non-hematopoietic cells and strongly expressed on macrophages. CD58 mediates cell adhesion by binding to CD2; (ANTIGENS, CD2); and this enhances antigen-specific T-cell activation.Peanut Hypersensitivity: Allergic reaction to peanuts that is triggered by the immune system.Immunophenotyping: Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.Filarioidea: A superfamily of nematodes of the suborder SPIRURINA. Its organisms possess a filiform body and a mouth surrounded by papillae.Antigens, CD13: Zinc-binding metalloproteases that are members of the type II integral membrane metalloproteases. They are expressed by GRANULOCYTES; MONOCYTES; and their precursors as well as by various non-hematopoietic cells. They release an N-terminal amino acid from a peptide, amide or arylamide.Anti-Allergic Agents: Agents that are used to treat allergic reactions. Most of these drugs act by preventing the release of inflammatory mediators or inhibiting the actions of released mediators on their target cells. (From AMA Drug Evaluations Annual, 1994, p475)Cytokines: Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.Betula: A plant genus of the family BETULACEAE. The tree has smooth, resinous, varicolored or white bark, marked by horizontal pores (lenticels), which usually peels horizontally in thin sheets.Antigens, CD11a: An alpha-integrin subunit found on lymphocytes, granulocytes, macrophages and monocytes. It combines with the integrin beta2 subunit (CD18 ANTIGEN) to form LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1.Skin Tests: Epicutaneous or intradermal application of a sensitizer for demonstration of either delayed or immediate hypersensitivity. Used in diagnosis of hypersensitivity or as a test for cellular immunity.Complement C5a: The minor fragment formed when C5 convertase cleaves C5 into C5a and COMPLEMENT C5B. C5a is a 74-amino-acid glycopeptide with a carboxy-terminal ARGININE that is crucial for its spasmogenic activity. Of all the complement-derived anaphylatoxins, C5a is the most potent in mediating immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE), smooth MUSCLE CONTRACTION; HISTAMINE RELEASE; and migration of LEUKOCYTES to site of INFLAMMATION.Hypersensitivity, Immediate: Hypersensitivity reactions which occur within minutes of exposure to challenging antigen due to the release of histamine which follows the antigen-antibody reaction and causes smooth muscle contraction and increased vascular permeability.Antigens, CD147: A widely distributed cell surface transmembrane glycoprotein that stimulates the synthesis of MATRIX METALLOPROTEINASES. It is found at high levels on the surface of malignant NEOPLASMS and may play a role as a mediator of malignant cell behavior.Urticaria: A vascular reaction of the skin characterized by erythema and wheal formation due to localized increase of vascular permeability. The causative mechanism may be allergy, infection, or stress.Cell SeparationAntigens, Plant: Substances found in PLANTS that have antigenic activity.Receptors, CCR3: CCR receptors with specificity for CHEMOKINE CCL11 and a variety of other CC CHEMOKINES. They are expressed at high levels in T-LYMPHOCYTES; EOSINOPHILS; BASOPHILS; and MAST CELLS.Antigens, CD82: A widely expressed transmembrane glycoprotein that functions as a METASTASIS suppressor protein. It is underexpressed in a variety of human NEOPLASMS.N-Formylmethionine Leucyl-Phenylalanine: A formylated tripeptide originally isolated from bacterial filtrates that is positively chemotactic to polymorphonuclear leucocytes, and causes them to release lysosomal enzymes and become metabolically activated.Interleukin-13: A cytokine synthesized by T-LYMPHOCYTES that produces proliferation, immunoglobulin isotype switching, and immunoglobulin production by immature B-LYMPHOCYTES. It appears to play a role in regulating inflammatory and immune responses.Leukocyte Count: The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.Immunologic Memory: The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus.Food Hypersensitivity: Gastrointestinal disturbances, skin eruptions, or shock due to allergic reactions to allergens in food.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Chemokine CCL11: A CC-type chemokine that is specific for CCR3 RECEPTORS. It is a potent chemoattractant for EOSINOPHILS.
2002). "Flow cytometric basophil activation test by detection of CD63 expression in patients with immediate-type reactions to ...
This protein has also been used in conjunction with CD63 as a marker for activated basophils in the Basophil Activation Test ... Bühring HJ, Seiffert M, Giesert C, Marxer A, Kanz L, Valent P, Sano K (May 2001). "The basophil activation marker defined by ... Bühring HJ, Streble A, Valent P (April 2004). "The basophil-specific ectoenzyme E-NPP3 (CD203c) as a marker for cell activation ... Ocmant A, Peignois Y, Mulier S, Hanssens L, Michils A, Schandené L (March 2007). "Flow cytometry for basophil activation ...
... is a good marker for flow cytometric quantification of in vitro activated basophils for diagnosis of IgE-mediated allergy ... In cell biology, CD63 is often used as a marker for multivessicular bodies, which are enriched with CD63. CD63 has been shown ... CD63 antigen is a protein that in humans is encoded by the CD63 gene. CD63 is mainly associated with membranes of intracellular ... CD63 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH) Human CD63 genome location and CD63 ...
Recently, Heneberg proposed that basophils may be defined as the cellular population positive for CD13, CD44, CD54, CD63, CD69 ... Basopenia (a low basophil count) is difficult to demonstrate as the normal basophil count is so low; it has been reported in ... Basophils are a type of white blood cells. Basophils are the least common of the granulocytes, representing about 0.5 to 1% of ... This suggests that basophils may play a role in the immune response to these viruses. Basophils of mice and humans have ...
Several minutes at room temperature gives the marker time to bind to the CD63 proteins on the cell membrane of the basophil. A ... The CD63 marker is an FITC labeled antigen which can bind to an CD63 protein and is used to sort the cells via FACS( ... including Mast cells and Basophils. Basophils contain many granules inside the cell, which are filled with a variety of active ... Pilot study on basophil activation induced by contrast medium. Fundam Clin Pharmacol 2011;25:267 - 276 Janeway CA, Jr.; et al ...
Recently, Heneberg[17] proposed that basophils may be defined as the cellular population positive for CD13, CD44, CD54, CD63, ... "Basophil". medcell.med.yale.edu.. *^ Voehringer D (December 2009). "The role of basophils in helminth infection". Trends in ... The word basophil uses combining forms of baso- + -phil, yielding "base-loving". ... Basophils are a type of white blood cell. Basophils are the least common of the granulocytes, representing about 0.5 to 1% of ...
A deficiency of CD63 can be associated with Hermansky-Pudlak syndrome.[8] The patients with this disease show signs of abnormal ... Since dense granules have surface membrane proteins, the activation of CD63 and LAMP-2 can be observed with flow cytometry. ... Dense granules are similar to lysosomes with an acidic pH and even some lysosomal proteins like CD63.[7] There is a granular ... "The protein CD63 is in platelet dense granules, is deficient in a patient with Hermansky-Pudlak syndrome, and appears ...
Shiratori I., Yamaguchi M., Suzukawa M. et al. Down-regulation of basophil function by human CD200 and human herpesvirus-8 ... CD63. *CD64 (A, B, C). *CD66 (a, b, c, d, e, f) ...
... basophils, and Th2 cells. DP2 activation also stimulates eosinophils and basophils to release the many pro-allergic elements of ... basophils, and Th2 cells at sites of nascent inflammation in animal models.[11] PGD2, acting through DP2, stimulates the in ... in human eosinophils and basophils". The Journal of Biological Chemistry. 279 (9): 7663-70. doi:10.1074/jbc.M310270200. PMID ... DP2 messenger RNA is also expressed by human basophils, eosinophils, a subpopulation of cytotoxic T cells (i.e. CD8+ T cells), ...
... basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial ...
The VCAM-1 protein mediates the adhesion of lymphocytes, monocytes, eosinophils, and basophils to vascular endothelium. It also ...
... discovery of activation markers such as CD63 and unique markers identifying basophil granulocytes. ... Basophil activation test measures basophil response to allergen cross-linking IgE on between 150 and 2000 basophil granulocytes ... The nature of basophil activation as an ex vivo challenge makes it a multifaceted and promising tool for the allergist. In this ... Basophil activation test may use fewer resources and be more reproducible than challenge testing. As it is less stressful for ...
Heparin blood basophil activation was similar through CD63 and CD203c. Basophils were significantly more sensitized three weeks ... and were stained with CD203c and CD63. Basophils were identified as CD203c+ leukocytes, and the proportion of CD63+ and CD203c ... Basophil sensitivity through CD63 or CD203c is a functional measure for specific immunotherapy. Susan Mikkelsen,1 Bo Martin ... Change in basophil activation measured as CD63 or CD203c activation at 0,1 ug/ml allergen did not vary significantly during the ...
Flow cytometric basophil activation test by detection of CD63 expression in patients with immediate-type reactions to ... Flow cytometric basophil activation test by detection of CD63 expression in patients with immediate-type reactions to ... Flow cytometric basophil activation test by detection of CD63 expression in patients with immediate-type reactions to ... In this study, we used flow cytometry to determine the percentage of activated basophils that expressed the CD63 marker after ...
Several minutes at room temperature gives the marker time to bind to the CD63 proteins on the cell membrane of the basophil. A ... The CD63 marker is an FITC labeled antigen which can bind to an CD63 protein and is used to sort the cells via FACS( ... including Mast cells and Basophils. Basophils contain many granules inside the cell, which are filled with a variety of active ... Pilot study on basophil activation induced by contrast medium. Fundam Clin Pharmacol 2011;25:267 - 276 Janeway CA, Jr.; et al ...
A. CD63 upregulation induced by egg white allergen (EGG1-4) at indicated concentrations. B. CD63 upregulation induced by peanut ... The distribution of percent CD63high basophils after in vitro stimulation from subjects over time by treatment status during ... The distribution of percent CD63high basophils after in vitro stimulation from subjects over time by treatment status during ... The distribution of percent CD63high basophils after in vitro stimulation from egg-allergic subjects (n=8) receiving active ...
Positive CD63 Basophil Activation Tests Are Common in Children with Chronic Spontaneous Urticaria and Linked to High Disease ... Positive CD63 Basophil Activation Tests Are Common in Children with Chronic Spontaneous Urticaria and Linked to High Disease ... Netchiporouk E, Moreau L, Rahme E, Maurer M, Lejtenyi D, Ben-Shoshan M. Positive CD63 Basophil Activation Tests Are Common in ... "Our study provides, for the time, reference and cutoff values for the CD63 BAT in children. Our findings show that positive ...
Heparin blood basophil activation was similar through CD63 and CD203c. Basophils were significantly more sensitized three weeks ... Basophils were stimulated at 7 log dilutions of V. vespula allergen for 15 min, and were stained with CD203c and CD63. ... Expression of CD203c was compared to CD63 as marker for basophil activation, using a Bland Altman plot and ROC curves. Patients ... Blood basophil concentration was marginally reduced by SCIT. Basophil activation is a versatile and sensitive tool that ...
Antigens CD63. Basophil degranulation test/methods. Basophils. Drug hypersensitivity/blood. Drug hypersensitivity/diagnosis. ... and observing basophil activation (indication of hypersensitivity) through upregulation of CD63 (or other basophil activation ... Looking for a safer tool, Basophil Activation Test (BAT) for allergy diagnosis has been studied in the last years. It is an in ... Antígenos CD63. Antiinflamatórios não esteróides. Basófilos. Curva ROC. Hipersensibilidade a drogas/diagnóstico. ...
A new variant of the basophil activation test for allergen-induced basophil CD63 upregulation. The effect of cetirizine. ... Diclofenac induces basophil degranulation without increasing CD63 expression in sensitive patients.. Malbrán A, Yeyati E, Rey ... Flow cytometric basophil activation test by detection of CD63 expression in patients with immediate-type reactions to ... Human basophil activation measured by CD63 expression and LTC4 release in IgE-mediated food allergy. ...
Furthermore, an increased activation of peripheral basophils was identified in MRL-lpr/lpr mice. Importantly, basophil-depleted ... basophil-adoptive-transferred mice exhibited the opposite results. Conclusion: These finding suggest that basophil activation- ... This study aimed to investigate the role of basophil activation in the development of SLE based on studies in patients with SLE ... The decrease of peripheral basophils in patients with SLE might be due to their migration to lymph nodes post their activation ...
Recently, Heneberg proposed that basophils may be defined as the cellular population positive for CD13, CD44, CD54, CD63, CD69 ... Basopenia (a low basophil count) is difficult to demonstrate as the normal basophil count is so low; it has been reported in ... Basophils are a type of white blood cells. Basophils are the least common of the granulocytes, representing about 0.5 to 1% of ... This suggests that basophils may play a role in the immune response to these viruses. Basophils of mice and humans have ...
Comparison of serum of visit 1 and 6 on third party basophils (CD63 upregulation on basophils) ... Change in the basophil proteome. *Change in basophil proteome in responders to omalizumab compared to non-responders to ... Effect of Omalizumab (Xolair) on Basophils in Patients With Chronic Idiopathic Urticaria. *Chronic Urticaria ... Fc-IgE Receptor density change on basophils. *Change of responsiveness to Fc-IgE cross-linking dependent stimuli (anti-IgE, ...
Purpose of Review The aim of this study is to critically review the relevant literature published on basophil activation test, ... Use of CD63 expression as a marker of in vitro basophil activation and leukotriene determination in metamizol allergic patients ... Eberlein B, León Suárez I, Darsow U, Ruëff F, Behrendt H, Ring J. A new basophil activation test using CD63 and CCR3 in allergy ... Comparison of two basophil activation markers CD63 and CD203c in the diagnosis of amoxicillin allergy. Clin Exp Allergy. 2008; ...
BACKGROUND: Basophil activation is associated with the expression of CD63. Because allergens can induce basophil activation by ... CD63 expression on basophils as a tool for the diagnosis of pollen-associated ... ... OBJECTIVE: We compared the CD63-based basophil activation test (BAT) in the diagnosis of allergy ... ... cross-linking specific IgE, increased CD63 expression has been proposed as a novel in vitro test for immediate type allergy. ...
IgE to mast cells and basophils. - mast cells, basophils and eosinophils cause release of histamine, leukotrienes, ... type I anaphylactic = basophils and eosinophils type II cytotoxic = antibody and complement. type III immune complex = ... Certain substances (makers) inside the basophil cell either come out of the cell or increase in number when the cell is ... IgE antibody mediated mast cell and basophil degranulation - release of pre-formed and de novo synthesised inflammatory ...
CD63 and CD203 expression by flow cytometry *​Newer tests. *Markers of basophil activation ... Newer laboratory tests that may assist in diagnosis include flow cytometry tests for expression of CD63 and CD203, markers of ... basophil activation. Referral to an allergist for allergen testing is recommended. Individuals with mastocytosis appear to be ...
Comparison of basophil activation tests using CD63 or CD203c expression in patients with insect venom allergy. Allergy 61: 1084 ... Comments regarding "Marked improvement of the basophil activation test by detecting CD203c instead of CD63" by Boumiza et al. ... tetraspanin CD63 has long been known as an activation marker of human basophils (29, 30). In contrast to other mast cell ... CD63 is a diagnostic marker in allergic diseases (29, 30), and the granular isoform of CD63 has also been reported as a ...
Knol EF, Mul FP, Jansen H, Calafat J, and Roos D. Monitoring human basophil activation via CD63 monoclonal antibody 435. J ... Basophil activation in unfractionated samples such as PBMC (note basophils are less dense than Ficoll) or whole blood can be ... Prepare Basophil medium (BM) as follows: *Add 5 μL of IL-3 into 2.5 mL of warm RPMI (use 5 mL PP tube). Vortex ... Evaluation of basophil activation in food allergy: present and future applications. Curr Opin Allergy Clin Immunol. 2006 Jun;6( ...
Knol EF, Mul FP, Jansen H, Calafat J, and Roos D. Monitoring human basophil activation via CD63 monoclonal antibody 435. J ... Prepare Basophil medium (BM) as follows: *Add 10 μL of IL-3 into 5 mL of warm RPMI (use 15 mL conical tube). Vortex ... Basophil activation in unfractionated samples such as whole blood can be measured by changes in the expression of cell surface ... Evaluation of basophil activation in food allergy: present and future applications. Curr Opin Allergy Clin Immunol. 2006 Jun;6( ...
CD63 is located on the basophilic granule membranes in resting basophils, mast cells, and platelets, and is also located on the ... CD63 functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. It ... CD63 belongs to the tetraspanin transmembrane 4 superfamily (TM4SF) of proteins, which are characterized by four membrane ... Clone REA444 recognizes the rat CD63 antigen, a multi-pass membrane protein which is also known as mast cell antigen AD1 or ...
AIU was diagnosed according to the basophil CD63 assay positivity.. The researchers found that ASST positivity, the occurrence ...
Keywords: Anaphylaxis; CD63; NSAID; asthma; basophil activation; diagnostic test; drug hypersensitivity; in vitro; intolerance ... A Basophil Activation Index (percentage of activated basophils after allergen stimulation/percentage of basally activated ... The basophil activation test is useful for the in vitro diagnosis of NSAID hypersensitivity, providing good specificity and ... Basophil activation test for the in vitro diagnosis of nonsteroidal anti-inflammatory drug hypersensitivity ...
... using flow cytometry has supplanted traditional methods of measuring basophil degranulation using histamine and other me ... 1991;88(3 Pt 1):328-38 Seminal paper identifying CD63 as a viable surrogate marker for basophil degranulation. CD63 is now a ... basophil sensitivity or both [33]. Basophil reactivity can be measured using %CD63+ basophils at a given concentration or using ... Priming of basophils with IL-3 can help to enhance IgE-mediated CD63 responses to allergen but can induce non-specific ...
Role of the CD63 Expression Test on Basophils in Drug Allergy Violeta Kvedariene,Bernadrd Arnoux and Pascal Demoly ...
Role of the CD63 Expression Test on Basophils in Drug Allergy Violeta Kvedariene,Bernadrd Arnoux and Pascal Demoly ...
  • ED7043-3 Staining Reagent - 1 vial containing 2 ml of premixed antibody cocktail: anti-CD63, FITC labeled + anti-CD203c, PE labeled. (exbio.cz)
  • Bazofil aktivasyonunu flow sitometride değerlendirmek için anti CD 123 PE, anti HLA-DR Per CP, anti CD63 FITC monoklonal antikor kombinasyonu kullanıldı.OSDT pozitif ürtiker hastalarında, atopik birey kanları ile inkübe edilen serumlarda CD63 ekspresyonu ile anlamlı ilişki bulundu (p: 0,033, r: 0,348). (uludag.edu.tr)
  • Anti CD123 PE, anti HLA-DR Per CP, anti CD63 FITC monoclonal antibody combinations were used to assess the basophil activation in flow cytometry.Statistically significant relationship was found between the ASST positive urticaria patients whose sera were incubated with the bloods of the atopic individuals and serum CD63 expression (p: 0,033, r: 0,348). (uludag.edu.tr)
  • Pre-OIT basophil reactivity positively associated with occurrence of symptoms during OIT, whereas the baseline milk IgE/total IgE ratio correlated with the likelihood of achieving sustained unresponsiveness. (nih.gov)
  • Combining omalizumab therapy with milk OIT led to distinct alterations in basophil reactivity but not T-cell responses. (nih.gov)
  • We sought to assess the utility of the basophil activation test (BAT) to predict the severity and threshold of reactivity to peanut during oral food challenges (OFCs). (ovid.com)
  • In addition, the absolute number of basophils was unchanged after hemodialysis with either of the dialyzers and compared with healthy controls. (pubfacts.com)
  • Mantegazza AR, Barrio MM, Moutel S, Bover L, Weck M, Brossart P, Teillaud JL, Mordoh J: CD63 tetraspanin slows down cell migration and translocates to theendosomal‑lysosomal‑MIICs route after extracellular stimuli in human immature dendritic cells. (sysmex-flowcytometry.com)
  • Herpesvirus-6, herpesvirus-7, and herpesvirus-8 produce a CD200 homolog which also inhibits basophil function. (wikipedia.org)
  • Peanut OIT induces a hyporesponsive state in basophils that is consistent with pathway-specific anergy previously described in vitro. (nih.gov)