A CCAAT-enhancer-binding protein found in LIVER; ADIPOSE TISSUE; INTESTINES; LUNG; ADRENAL GLANDS; PLACENTA; OVARY and peripheral blood mononuclear cells (LEUKOCYTES, MONONUCLEAR). Experiments with knock-out mice have demonstrated that CCAAT-enhancer binding protein-alpha is essential for the functioning and differentiation of HEPATOCYTES and ADIPOCYTES.
A class of proteins that were originally identified by their ability to bind the DNA sequence CCAAT. The typical CCAAT-enhancer binding protein forms dimers and consists of an activation domain, a DNA-binding basic region, and a leucine-rich dimerization domain (LEUCINE ZIPPERS). CCAAT-BINDING FACTOR is structurally distinct type of CCAAT-enhancer binding protein consisting of a trimer of three different subunits.
A CCAAT-enhancer-binding protein found in LIVER; INTESTINES; LUNG and ADIPOSE TISSUE. It is an important mediator of INTERLEUKIN-6 signaling.
A member of the C-EBP protein family of transcription factors. It plays a key role in G0 PHASE mammary EPITHELIAL CELL growth arrest, and it is involved in transcriptional regulation of INTERLEUKIN 1; INTERLEUKIN 6; and TUMOR NECROSIS FACTOR-ALPHA.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
A CCAAT-enhancer binding protein that is induced by DNA DAMAGE and growth arrest. It serves as a dominant negative inhibitor of other CCAAT-enhancer binding proteins.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Cells in the body that store FATS, usually in the form of TRIGLYCERIDES. WHITE ADIPOCYTES are the predominant type and found mostly in the abdominal cavity and subcutaneous tissue. BROWN ADIPOCYTES are thermogenic cells that can be found in newborns of some species and hibernating mammals.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The differentiation of pre-adipocytes into mature ADIPOCYTES.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.
Established cell cultures that have the potential to propagate indefinitely.
A continuous cell line that is a substrain of SWISS 3T3 CELLS developed though clonal isolation. The mouse fibroblast cells undergo an adipose-like conversion as they move to a confluent and contact-inhibited state.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. These elements may be found in both promoter and enhancer regions.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
A nuclear transcription factor. Heterodimerization with RETINOID X RECEPTOR ALPHA is important in regulation of GLUCOSE metabolism and CELL GROWTH PROCESSES. It is a target of THIAZOLIDINEDIONES for control of DIABETES MELLITUS.
Formation of MYELOID CELLS from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW via MYELOID STEM CELLS. Myelopoiesis generally refers to the production of leukocytes in blood, such as MONOCYTES and GRANULOCYTES. This process also produces precursor cells for MACROPHAGE and DENDRITIC CELLS found in the lymphoid tissue.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
DNA-binding motifs formed from two alpha-helixes which intertwine for about eight turns into a coiled coil and then bifurcate to form Y shaped structures. Leucines occurring in heptad repeats end up on the same sides of the helixes and are adjacent to each other in the stem of the Y (the "zipper" region). The DNA-binding residues are located in the bifurcated region of the Y.
Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.
Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.
An organochlorine compound that was formerly used as an insecticide. Its manufacture and use has been discontinued in the United States. (From Merck Index, 11th ed)
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Nucleic acid sequences involved in regulating the expression of genes.
A forkhead transcription factor that regulates expression of metabolic GENES and is involved in EMBRYONIC DEVELOPMENT. Mutations in HNF-3beta have been associated with CONGENITAL HYPERINSULINISM.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
An early local inflammatory reaction to insult or injury that consists of fever, an increase in inflammatory humoral factors, and an increased synthesis by hepatocytes of a number of proteins or glycoproteins usually found in the plasma.
A component of NF-kappa B transcription factor. It is proteolytically processed from NF-kappa B p105 precursor protein and is capable of forming dimeric complexes with itself or with TRANSCRIPTION FACTOR RELA. It regulates expression of GENES involved in immune and inflammatory responses.
Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.
A DNA-directed RNA polymerase found in BACTERIA. It is a holoenzyme that consists of multiple subunits including sigma factor 54.
A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. This protein functions to integrate both calcium and cAMP signals.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
An anti-inflammatory 9-fluoro-glucocorticoid.
A human cell line established from a diffuse histiocytic lymphoma (HISTIOCYTIC LYMPHOMA, DIFFUSE) and displaying many monocytic characteristics. It serves as an in vitro model for MONOCYTE and MACROPHAGE differentiation.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A family of DNA binding proteins that regulate expression of a variety of GENES during CELL DIFFERENTIATION and APOPTOSIS. Family members contain a highly conserved carboxy-terminal basic HELIX-TURN-HELIX MOTIF involved in dimerization and sequence-specific DNA binding.
A cell line derived from cultured tumor cells.
Transport proteins that carry specific substances in the blood or across cell membranes.
Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA.
Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)
Intracellular receptors that can be found in the cytoplasm or in the nucleus. They bind to extracellular signaling molecules that migrate through or are transported across the CELL MEMBRANE. Many members of this class of receptors occur in the cytoplasm and are transported to the CELL NUCLEUS upon ligand-binding where they signal via DNA-binding and transcription regulation. Also included in this category are receptors found on INTRACELLULAR MEMBRANES that act via mechanisms similar to CELL SURFACE RECEPTORS.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A group of CORTICOSTEROIDS that affect carbohydrate metabolism (GLUCONEOGENESIS, liver glycogen deposition, elevation of BLOOD SUGAR), inhibit ADRENOCORTICOTROPIC HORMONE secretion, and possess pronounced anti-inflammatory activity. They also play a role in fat and protein metabolism, maintenance of arterial blood pressure, alteration of the connective tissue response to injury, reduction in the number of circulating lymphocytes, and functioning of the central nervous system.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in leukemia.
Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.
A multiprotein complex composed of the products of c-jun and c-fos proto-oncogenes. These proteins must dimerize in order to bind to the AP-1 recognition site, also known as the TPA-responsive element (TRE). AP-1 controls both basal and inducible transcription of several genes.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Various physiological or molecular disturbances that impair ENDOPLASMIC RETICULUM function. It triggers many responses, including UNFOLDED PROTEIN RESPONSE, which may lead to APOPTOSIS; and AUTOPHAGY.
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.
A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.
Parent cells in the lineage that gives rise to MONOCYTES and MACROPHAGES.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A large group of membrane transport proteins that shuttle MONOSACCHARIDES across CELL MEMBRANES.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
A primary malignant neoplasm of epithelial liver cells. It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. The cells may be uniform or markedly pleomorphic, or form GIANT CELLS. Several classification schemes have been suggested.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
Proteins that bind to the 3' polyadenylated region of MRNA. When complexed with RNA the proteins serve an array of functions such as stabilizing the 3' end of RNA, promoting poly(A) synthesis and stimulating mRNA translation.
Intracellular proteins that reversibly bind hydrophobic ligands including: saturated and unsaturated FATTY ACIDS; EICOSANOIDS; and RETINOIDS. They are considered a highly conserved and ubiquitously expressed family of proteins that may play a role in the metabolism of LIPIDS.
An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. This catalyzes endonucleolytic cleavage of DNA yielding 5'-phosphodi- and oligonucleotide end-products. The enzyme has a preference for double-stranded DNA.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Solutions that have a greater osmotic pressure than a reference solution such as blood, plasma, or interstitial fluid.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
A method for determining the sequence specificity of DNA-binding proteins. DNA footprinting utilizes a DNA damaging agent (either a chemical reagent or a nuclease) which cleaves DNA at every base pair. DNA cleavage is inhibited where the ligand binds to DNA. (from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Tumors or cancer of the LIVER.
A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).
Proteins prepared by recombinant DNA technology.
A sterol regulatory element binding protein that regulates expression of GENES involved in FATTY ACIDS metabolism and LIPOGENESIS. Two major isoforms of the protein exist due to ALTERNATIVE SPLICING.
Elements of limited time intervals, contributing to particular results or situations.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
A heterotrimeric DNA-binding protein that binds to CCAAT motifs in the promoters of eukaryotic genes. It is composed of three subunits: A, B and C.
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.
The process by which two molecules of the same chemical composition form a condensation product or polymer.
Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
A species of gliding bacteria found on soil as well as in surface fresh water and coastal seawater.
Repair or renewal of hepatic tissue.
A theoretical representative nucleotide or amino acid sequence in which each nucleotide or amino acid is the one which occurs most frequently at that site in the different sequences which occur in nature. The phrase also refers to an actual sequence which approximates the theoretical consensus. A known CONSERVED SEQUENCE set is represented by a consensus sequence. Commonly observed supersecondary protein structures (AMINO ACID MOTIFS) are often formed by conserved sequences.
Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL).
A family of transcription factors characterized by the presence of highly conserved calcineurin- and DNA-binding domains. NFAT proteins are activated in the CYTOPLASM by the calcium-dependent phosphatase CALCINEURIN. They transduce calcium signals to the nucleus where they can interact with TRANSCRIPTION FACTOR AP-1 or NF-KAPPA B and initiate GENETIC TRANSCRIPTION of GENES involved in CELL DIFFERENTIATION and development. NFAT proteins stimulate T-CELL activation through the induction of IMMEDIATE-EARLY GENES such as INTERLEUKIN-2.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
Promoter-specific RNA polymerase II transcription factor that binds to the GC box, one of the upstream promoter elements, in mammalian cells. The binding of Sp1 is necessary for the initiation of transcription in the promoters of a variety of cellular and viral GENES.
Deletion of sequences of nucleic acids from the genetic material of an individual.
A T-cell factor that plays an essential role in EMBRYONIC DEVELOPMENT.
A family of immunophilin proteins that bind to the immunosuppressive drugs TACROLIMUS (also known as FK506) and SIROLIMUS. EC 5.2.1.-
The artificial induction of GENE SILENCING by the use of RNA INTERFERENCE to reduce the expression of a specific gene. It includes the use of DOUBLE-STRANDED RNA, such as SMALL INTERFERING RNA and RNA containing HAIRPIN LOOP SEQUENCE, and ANTI-SENSE OLIGONUCLEOTIDES.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
MAMMARY GLANDS in the non-human MAMMALS.
A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain. Runx1 is frequently mutated in human LEUKEMIAS.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
A histone deacetylase subtype that is found along with HISTONE DEACETYLASE 2; RETINOBLASTOMA-BINDING PROTEIN 4; and RETINOBLASTOMA-BINDING PROTEIN 7 as core components of histone deacetylase complexes.
An interleukin-1 subtype that is synthesized as an inactive membrane-bound pro-protein. Proteolytic processing of the precursor form by CASPASE 1 results in release of the active form of interleukin-1beta from the membrane.
A poly(A) binding protein that has a variety of functions such as mRNA stabilization and protection of RNA from nuclease activity. Although poly(A) binding protein I is considered a major cytoplasmic RNA-binding protein it is also found in the CELL NUCLEUS and may be involved in transport of mRNP particles.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.
A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from http://www.atcc.org/)
A family of double-stranded RNA-binding proteins that are related to NFATC TRANSCRIPTION FACTORS. In addition to binding to RNA, nuclear factor 90 proteins form heterodimeric complexes that regulate GENETIC TRANSCRIPTION and may play a role in T-CELL activation.
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
Activating transcription factors of the MADS family which bind a specific sequence element (MEF2 element) in many muscle-specific genes and are involved in skeletal and cardiac myogenesis, neuronal differentiation and survival/apoptosis.
A family of soluble proteins that bind insulin-like growth factors and modulate their biological actions at the cellular level. (Int J Gynaecol Obstet 1992;39(1):3-9)
Proteins found in any species of bacterium.
Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.
A cellular response to environmental insults that cause disruptions in PROTEIN FOLDING and/or accumulation of defectively folded protein in the ENDOPLASMIC RETICULUM. It consists of a group of regulatory cascades that are triggered as a response to altered levels of calcium and/or the redox state of the endoplasmic reticulum. Persistent activation of the unfolded protein response leads to the induction of APOPTOSIS.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
A family of muscle-specific transcription factors which bind to DNA in control regions and thus regulate myogenesis. All members of this family contain a conserved helix-loop-helix motif which is homologous to the myc family proteins. These factors are only found in skeletal muscle. Members include the myoD protein (MYOD PROTEIN); MYOGENIN; myf-5, and myf-6 (also called MRF4 or herculin).
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.
A 16-kDa peptide hormone secreted from WHITE ADIPOCYTES. Leptin serves as a feedback signal from fat cells to the CENTRAL NERVOUS SYSTEM in regulation of food intake, energy balance, and fat storage.
Tumor necrosis factor receptor family members that are widely expressed and play a role in regulation of peripheral immune responses and APOPTOSIS. The receptors are specific for TNF-RELATED APOPTOSIS-INDUCING LIGAND and signal via conserved death domains that associate with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
Cellular DNA-binding proteins encoded by the c-jun genes (GENES, JUN). They are involved in growth-related transcriptional control. There appear to be three distinct functions: dimerization (with c-fos), DNA-binding, and transcriptional activation. Oncogenic transformation can take place by constitutive expression of c-jun.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions.
A conserved A-T rich sequence which is contained in promoters for RNA polymerase II. The segment is seven base pairs long and the nucleotides most commonly found are TATAAAA.
The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.
Sequences of DNA in the genes that are located between the EXONS. They are transcribed along with the exons but are removed from the primary gene transcript by RNA SPLICING to leave mature RNA. Some introns code for separate genes.
A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.
Cis-acting regulatory sequences in the HIV long terminal repeat (LTR) which play a major role in induction or augmentation of HIV gene expression in response to environmental stimuli such as mitogens, phorbol esters, or other viruses. The HIV enhancer is the binding site for many cellular transcription factors including the nuclear factor NF-kappa B.
A poly(A) binding protein that is involved in promoting the extension of the poly A tails of MRNA. The protein requires a minimum of ten ADENOSINE nucleotides in order for binding to mRNA. Once bound it works in conjunction with CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR to stimulate the rate of poly A synthesis by POLY A POLYMERASE. Once poly-A tails reach around 250 nucleotides in length poly(A) binding protein II no longer stimulates POLYADENYLATION. Mutations within a GCG repeat region in the gene for poly(A) binding protein II have been shown to cause the disease MUSCULAR DYSTROPHY, OCULOPHARYNGEAL.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.
Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Progenitor cells from which all blood cells derive.
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
A family of transcription factors that bind to the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. Family members contain a highly conserved DNA-binding domain known as the runt domain. They can act as both activators and repressors of expression of GENES involved in CELL DIFFERENTIATION and CELL CYCLE progression.
One of the six homologous soluble proteins that bind insulin-like growth factors (SOMATOMEDINS) and modulate their mitogenic and metabolic actions at the cellular level.
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
Periplasmic proteins that scavenge or sense diverse nutrients. In the bacterial environment they usually couple to transporters or chemotaxis receptors on the inner bacterial membrane.
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
A member of the p300-CBP transcription factor family that was initially identified as a binding partner for CAMP RESPONSE ELEMENT-BINDING PROTEIN. Mutations in CREB-binding protein are associated with RUBINSTEIN-TAYBI SYNDROME.
Biochemical identification of mutational changes in a nucleotide sequence.
Y-box-binding protein 1 was originally identified as a DNA-binding protein that interacts with Y-box PROMOTER REGIONS of MHC CLASS II GENES. It is a highly conserved transcription factor that regulates expression of a wide variety of GENES.
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
The parts of a transcript of a split GENE remaining after the INTRONS are removed. They are spliced together to become a MESSENGER RNA or other functional RNA.
A 12-KDa tacrolimus binding protein that is found associated with and may modulate the function of calcium release channels. It is a peptidyl-prolyl cis/trans isomerase which is inhibited by both tacrolimus (commonly called FK506) and SIROLIMUS.
A superfamily of proteins containing the globin fold which is composed of 6-8 alpha helices arranged in a characterstic HEME enclosing structure.

C/EBPalpha regulates generation of C/EBPbeta isoforms through activation of specific proteolytic cleavage. (1/2395)

C/EBPalpha and C/EBPbeta are intronless genes that can produce several N-terminally truncated isoforms through the process of alternative translation initiation at downstream AUG codons. C/EBPbeta has been reported to produce four isoforms: full-length 38-kDa C/EBPbeta, 35-kDa LAP (liver-enriched transcriptional activator protein), 21-kDa LIP (liver-enriched transcriptional inhibitory protein), and a 14-kDa isoform. In this report, we investigated the mechanisms by which C/EBPbeta isoforms are generated in the liver and in cultured cells. Using an in vitro translation system, we found that LIP can be generated by two mechanisms: alternative translation and a novel mechanism-specific proteolytic cleavage of full-length C/EBPbeta. Studies of mice in which the C/EBPalpha gene had been deleted (C/EBPalpha-/-) showed that the regulation of C/EBPbeta proteolysis is dependent on C/EBPalpha. The induction of C/EBPalpha in cultured cells leads to induced cleavage of C/EBPbeta to generate the LIP isoform. We characterized the cleavage activity in mouse liver extracts and found that the proteolytic cleavage activity is specific to prenatal and newborn livers, is sensitive to chymostatin, and is completely abolished in C/EBPalpha-/- animals. The lack of cleavage activity in the livers of C/EBPalpha-/- mice correlates with the decreased levels of LIP in the livers of these animals. Analysis of LIP production during liver regeneration showed that, in this system, the transient induction of LIP is dependent on the third AUG codon and most likely involves translational control. We propose that there are two mechanisms by which C/EBPbeta isoforms might be generated in the liver and in cultured cells: one that is determined by translation and a second that involves C/EBPalpha-dependent, specific proteolytic cleavage of full-length C/EBPbeta. The latter mechanism implicates C/EBPalpha in the regulation of posttranslational generation of the dominant negative C/EBPbeta isoform, LIP.  (+info)

A critical role for cAMP response element-binding protein (CREB) as a Co-activator in sterol-regulated transcription of 3-hydroxy-3-methylglutaryl coenzyme A synthase promoter. (2/2395)

3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase, a key regulatory enzyme in the pathway for endogenous cholesterol synthesis, is a target for negative feedback regulation by cholesterol. When cellular sterol levels are low, the sterol regulatory element-binding proteins (SREBPs) are released from the endoplasmic reticulum membrane, allowing them to translocate to the nucleus and activate SREBP target genes. However, in all SREBP-regulated promoters studied to date, additional co-regulatory transcription factors are required for sterol-regulated activation of transcription. We have previously shown that, in addition to SREBPs, NF-Y/CBF is required for sterol-regulated transcription of HMG-CoA synthase. This heterotrimeric transcription factor has recently been shown to function as a co-regulator in several other SREBP-regulated promoters, as well. In addition to cis-acting sites for both SREBP and NF-Y/CBF, the sterol regulatory region of the synthase promoter also contains a consensus cAMP response element (CRE), an element that binds members of the CREB/ATF family of transcription factors. Here, we show that this consensus CRE is essential for sterol-regulated transcription of the synthase promoter. Using in vitro binding assays, we also demonstrate that CREB binds to this CRE, and mutations within the CRE that result in a loss of CREB binding also result in a loss of sterol-regulated transcription. We further show that efficient activation of the synthase promoter in Drosophila SL2 cells requires the simultaneous expression of all three factors: SREBPs, NF-Y/CBF, and CREB. To date this is the first promoter shown to require CREB for efficient sterol-regulated transcription, and to require two different co-regulatory factors in addition to SREBPs for maximal activation.  (+info)

CCAAT/enhancer-binding protein beta is an accessory factor for the glucocorticoid response from the cAMP response element in the rat phosphoenolpyruvate carboxykinase gene promoter. (3/2395)

The cyclic AMP response element (CRE) of the rat phosphoenolpyruvate carboxykinase (PEPCK) gene promoter is required for a complete glucocorticoid response. Proteins known to bind the PEPCK CRE include the CRE-binding protein (CREB) and members of the CCAAT/enhancer-binding protein (C/EBP) family. We took two different approaches to determine which of these proteins provides the accessory factor activity for the glucocorticoid response from the PEPCK CRE. The first strategy involved replacing the CRE of the PEPCK promoter/chloramphenicol acetyltransferase reporter plasmid (pPL32) with a consensus C/EBP-binding sequence. This construct, termed pDeltaCREC/EBP, binds C/EBPalpha and beta but not CREB, yet it confers a nearly complete glucocorticoid response when transiently transfected into H4IIE rat hepatoma cells. These results suggest that one of the C/EBP family members may be the accessory factor. The second strategy involved co-transfecting H4IIE cells with a pPL32 mutant, in which the CRE was replaced with a GAL4-binding sequence (pDeltaCREGAL4), and various GAL4 DNA-binding domain (DBD) fusion protein expression vectors. Although chimeric proteins consisting of the GAL4 DBD fused to either CREB or C/EBPalpha are able to confer an increase in basal transcription, they do not facilitate the glucocorticoid response. In contrast, a fusion protein consisting of the GAL4 DBD and amino acids 1-118 of C/EBPbeta provides a significant glucocorticoid response. Additional GAL4 fusion studies were done to map the minimal domain of C/EBPbeta needed for accessory factor activity to the glucocorticoid response. Chimeric proteins containing amino acid regions 1-84, 52-118, or 85-118 of C/EBPbeta fused to the GAL4 DBD do not mediate a glucocorticoid response. We conclude that the amino terminus of C/EBPbeta contains a multicomponent domain necessary to confer accessory factor activity to the glucocorticoid response from the CRE of the PEPCK gene promoter.  (+info)

CCAAT/enhancer-binding proteins. A role in regulation of human involucrin promoter response to phorbol ester. (4/2395)

The phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) is a potent inducer of keratinocyte differentiation and of involucrin gene expression. In the present study we show that a CCAAT/enhancer-binding protein (C/EBP) site in the proximal regulatory region is required for the phorbol ester response. Mutation of the C/EBP site results in the loss of basal and TPA-responsive activity. Gel mobility supershift analysis shows that C/EBPalpha binding to this site is increased by TPA treatment. Moreover, cotransfection of the human involucrin reporter plasmid with C/EBPalpha increases promoter activity to an extent comparable with TPA treatment. Mutation of the C/EBP-binding site eliminates these responses. Transfection experiments using GADD153 to create C/EBP-null conditions confirm that C/EBP factors are absolutely required for promoter activity and TPA responsiveness. C/EBPbeta and C/EBPdelta inhibit both TPA- and C/EBPalpha-dependent promoter activation, indicating functional differences among C/EBP family members. These results suggest that C/EBP transcription factor activity is necessary for basal promoter activity and TPA response of the involucrin gene.  (+info)

The mammalian endoplasmic reticulum stress response element consists of an evolutionarily conserved tripartite structure and interacts with a novel stress-inducible complex. (5/2395)

When mammalian cells are subjected to calcium depletion stress or protein glycosylation block, the transcription of a family of glucose-regulated protein (GRP) genes encoding endoplasmic reticulum (ER) chaperones is induced to high levels. The consensus mammalian ER stress response element (ERSE) conserved among grp promoters consists of a tripartite structure CCAAT(N9)CCACG, with N being a strikingly GC-rich region of 9 bp. The ERSE, in duplicate copies, can confer full stress inducibility to a heterologous promoter in a sequence-specific but orientation-independent manner. In addition to CBF/NF-Y and YY1 binding to the CCAAT and CCACG motifs, respectively, we further discovered that an ER stress-inducible complex (ERSF) from HeLa nuclear extract binds specifically to the ERSE. Strikingly, the interaction of the ERSF with the ERSE requires a conserved GGC motif within the 9 bp region. Since mutation of the GGC triplet sequence also results in loss of stress inducibility, specific sequence within the 9 bp region is an integral part of the tripartite structure. Finally, correlation of factor binding with stress inducibility reveals that ERSF binding to the ERSE alone is not sufficient; full stress inducibility requires integrity of the CCAAT, GGC and CCACG sequence motifs, as well as precise spacing among these sites.  (+info)

Tumour necrosis factor-alpha regulates expression of the CCAAT-enhancer-binding proteins (C/EBPs) alpha and beta and determines the occupation of the C/EBP site in the promoter of the insulin-responsive glucose-transporter gene in 3T3-L1 adipocytes. (6/2395)

We have demonstrated previously that treatment of 3T3-L1 adipocytes with tumour necrosis factor-alpha (TNF) results in a rapid (4 h) and significant (75-80%) reduction in the rate of transcription of the GLUT4 gene. Control of GLUT4 gene transcription has been suggested at least in part to reside with the CCAAT-enhancer-binding protein (C/EBP) family (alpha, beta and delta isoforms) of transcription factors. Using electrophoretic mobility shift assays, we have examined the ability of TNF to alter the occupation of the C/EBP site in the GLUT4 promoter. The data suggest that in fully differentiated adipocytes the C/EBP site is a ligand for predominantly alpha/alpha homodimers; however, after exposure to TNF, a shift in occupancy of the site occurs and the ligands become alpha/beta heterodimers and beta/beta homodimers. Partner selection in dimer formation appears to be controlled by selective translocation of the beta-isoform from the cytosol to the nucleus after exposure of the cells to TNF.  (+info)

A novel splicing isoform of mouse sterol regulatory element-binding protein-1 (SREBP-1). (7/2395)

We cloned a cDNA encoding the NH2-terminal portion of mouse SREBP-1. The deduced amino acid sequence was 76% and 90% identical to human and hamster SREBP-1, respectively. We found out a novel splicing isoform of mouse SREBP-1 that lacks 42 amino acid residues composing a PEST sequence observed in unstable proteins. It has been reported that SREBP-1 is rapidly turned over in the nucleus. Although this isoform was not a dominant isoform, it might be possible that the produced protein functions differently from other isoforms including a complete PEST sequence.  (+info)

Transcription factors CCAAT/enhancer-binding protein beta and nuclear factor-Y bind to discrete regulatory elements in the very low density lipoprotein receptor promoter. (8/2395)

Expression of the very low density lipoprotein receptor (VLDL-R) is barely detectable in liver, but occurs in adipose tissue, skeletal muscle, heart, and placenta, where it is postulated to supply triglyceride to tissues that utilize fatty acids. To investigate its tissue-specific expression, cell lines were transfected with luciferase reporter gene constructs driven by the 5'-flanking region of the VLDL-R gene. Transcriptional activity of a 4.2-kb promoter fragment was 5-fold higher in BeWo placental cells than in Huh-7 hepatoma cells, consistent with relative endogenous expression of the VLDL-R. By deletion analysis, DNase I protection assays and site-directed mutagenesis, two regulatory elements were essential for maximal promoter activity in BeWo cells: footprint site D (-856 to -830) and an inverted CCAAT box (-703 to -707). Mutation of either element reduced promoter activity by 60% in BeWo cells, but had little effect in Huh-7 cells, suggesting that these elements direct cell-type specific transcription. Electrophoretic mobility-shift assays with BeWo nuclear extracts revealed that the inverted CCAAT box binds transcription factor NF-Y, and site D binds CCAAT/enhancer-binding protein b (C/EBPbeta) and minor amounts of C/EBPalpha and C/EBPdelta. Overexpression of a dominant negative NF-YA vector confirmed involvement of NF-Y in the regulation of the VLDL-receptor gene through the CCAAT box. However overexpression of C/EBP could not stimulate transcription from the VLDL-receptor promoter nor from site D fused to a heterologous promoter, suggesting that the simultaneous binding of an accessory factor(s) may be necessary for C/EBP transactivation via the D site.  (+info)

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Monocytes are circulating, short-lived mononuclear phagocytes, which in mice and man comprise two main subpopulations. Murine Ly6C(+) monocytes display developmental plasticity and are recruited to complement tissue-resident macrophages and dendritic cells on demand. Murine vascular Ly6C(-) monocytes patrol the endothelium, act as scavengers, and support vessel wall repair. Here we characterized population and single cell transcriptomes, as well as enhancer and promoter landscapes of the murine monocyte compartment. Single cell RNA-seq and transplantation experiments confirmed homeostatic default differentiation of Ly6C(+) into Ly6C(-) monocytes. The main two subsets were homogeneous, but linked by a more heterogeneous differentiation intermediate. We show that monocyte differentiation occurred through de novo enhancer establishment and activation of pre-established (poised) enhancers. Generation of Ly6C(-) monocytes involved induction of the transcription factor C/EBP{beta} and ...
TY - JOUR. T1 - Identification of transcriptional activation and repression domains in human CCAAT/enhancer-binding protein ε. AU - Williamson, Elizabeth A.. AU - Xu, Haixin N.. AU - Gombart, Adrian F.. AU - Verbeek, Walter. AU - Chumakov, Alexey M.. AU - Friedman, Alan D.. AU - Koeffler, H. Phillip. PY - 1998/6/12. Y1 - 1998/6/12. N2 - Human CCAAT/enhancer-binding protein ε (C/EBPε), a new member of the C/EBP family, significantly upregulates both the mim-1 and human myeloperoxidase promoters, suggesting an important role for C/EBPε in the transcriptional regulation of a subset of myeloid-specific genes. To elucidate the structure and function of C/EBPε in transcriptional activation, amino acid residues 1-115, 147-249, or 1-249 of C/EBPε were fused to the yeast GAL4 DNA binding domain. These expression vectors were cotransfected with a chloramphenicol acetyltransferase reporter gene and, in all cell lines tested, only the GAL-C/EBPε-(1-115) fusion protein significantly activated ...
FIG. 4. Effect of site-specific mutations on C/EBP and NF-Y binding activity. Nuclear extracts were prepared from 293T cells overexpressing C/EBP-α or -β. For mock, the cells were transfected by pcDNA. All probes were generated by PCR with 32P-labeled antisense primer and unlabeled sense primers using site-directed mutated plasmids as templates, and purified from the polyacrylamide gel, to equalize the specific activities between probes. A: DNA oligonucleotide sequence of wild and mutated probes used in EMSA. Mutated regions are indicated by lowercase with * with their name. B: EMSA using nuclear extracts of 293T cells overexpression C/EBP-α and C/EBP-β. Wild-type (wt) and mutant (m1, m2, m3, m4, and m5) probes were incubated with 4 μg of indicated nuclear extracts. The bands corresponding to NF-Y, C/EBP-α, and C/EBP-β are marked by arrows. C: Western blot (WB) analysis of C/EBP-α and -β for validating the expression of C/EBP-α and -β. 293T cells were transfected with expression ...
TY - JOUR. T1 - Differentiation-induced gene expression in 3T3-L1 preadipocytes. T2 - CCAAT/enhancer binding protein interacts with and activates the promoters of two adipocyte-specific genes.. AU - Christy, R. J.. AU - Yang, V. W.. AU - Ntambi, J. M.. AU - Geiman, D. E.. AU - Landschulz, W. H.. AU - Friedman, A. D.. AU - Nakabeppu, Y.. AU - Kelly, T. J.. AU - Lane, M. D.. PY - 1989/9. Y1 - 1989/9. N2 - Previous studies have shown that differentiation of 3T3-L1 preadipocytes leads to the transcriptional activation of a group of adipose-specific genes. As an approach to defining the mechanism responsible for activating the expression of these genes, we investigated the binding of nuclear factors to the promoters of two differentiation-induced genes, the 422(aP2) and stearoyl-CoA desaturase 1 (SCD1) genes. DNase I footprinting and gel retardation analysis identified two binding regions within the promoters of each gene that interact with nuclear factors present in differentiated 3T3-L1 adipocytes. ...
Positively regulates Ras-mediated pathways. Acts downstream or parallel to Raf, but upstream of nuclear factors in Ras signaling. Three mutants have been isolated, that suppress the rough eye phenotype caused by mutated Ras1 (sev-Ras1 v12). Inhibits yki activity by restricting its nuclear localization.
The CCAAT/enhancer-binding protein β (C/EBPβ) is a transcription factor, which was first identified as a regulator of differentiation and inflammatory processes mainly in adipose tissue and liver; however, its function in the brain was largely unknown for many years. Previous studies from our laboratory indicated that C/EBPβ is implicated in inflammatory process and brain injury, since mice lacking this gene were less susceptible to kainic acid-induced injury. We first performed cDNA microarrays analysis using hippocampal RNA isolated from C/EBPβ +/+ and C/EBPβ −/− mice. Immunocytochemical and immunohistochemical studies were done to evaluate C/EBPβ and C3 levels. Transient transfection experiments were made to analyze transcriptional regulation of C3 by C/EBPβ. To knockdown C/EBPβ and C3 expression, mouse astrocytes were infected with lentiviral particles expressing an shRNA specific for C/EBPβ or an siRNA specific for C3. Among the genes displaying
In this work, we have shown that the transcription factor C/EBPβ directly regulates the expression of the C3 gene, and that this control could be relevant for the pro-inflammatory effects of this transcription factor. By microarray analysis and RT-PCR we showed that the hippocampal content of C3 transcripts was depleted in C/EBPβ −/− mice. The analysis of the C3 promoter showed that this gene was directly induced by C/EBPβ through a C/EBPβ consensus site located at −616/-599 position from the transcription start site. In accordance with these data, LPS induced the expression of C3 in glial cells, at least in part, through the induction of C/EBPβ since the repression of LPS-induction of C/EBPβ by shRNA interference blocked C3 increase. On the contrary, C/EBPβ overexpression by transient transfection induced C3 expression. Additionally, treatment of these cultures with LPS induced the levels of the pro-inflammatory factors IL-1β and COX-2, which were significantly reduced in those ...
In this study we have shown that the rat UDP-glucuronosyltransferase 2B1 gene is specifically activated by C/EBPα and that this activation is correlated with the binding of C/EBPα to an element residing between −91 and −99 bp upstream of the UGT2B1 gene transcription start site. Furthermore, we extend these findings to show that C/EBPα is essential for the expression of transcripts homologous to UGT2B1 in adult mouse liver.. This is the first example of the regulation of a UGT2B gene by a member of the C/EBP transcription factor family, and adds to our previous finding that the UGT2B1 gene promoter also interacts with and is activated by HNF1α (Hansen et al., 1997). Both C/EBP and HNF1 also interact with the early promoter of the albumin gene (Fig. 1) (Lichtsteiner et al., 1987). When these two factors are simultaneously overexpressed, there is a strong synergistic effect on transcription of this gene (Wu et al., 1994). It was shown that a specific C/EBPα activation domain was required ...
CEBPD (C/EBP delta) is a member of the CCAAT-enhancer binding protein (C/EBP) family of transcription factors characterized by a b-Zip domain that mediates dimerization and DNA binding. CEBPD is induced in response to acute stressors such as cytokine stimulation, bacterial lipopolysaccharide (LPS), corticosteroids, radiation and hypoxia. We have previously reported that CEBPD has dual functions in breast cancer by both attenuating or enhancing oncogenic pathways depending on context (Balamurugan and Sterneck, 2013, Mendoza-Villanueva et al., 2016). Recent studies reveal that elevated Endoplasmic Reticulum (ER) stress is associated with the pathology of several diseases including cancer. Limiting supply of nutrients and oxygen in growing tumor cells disrupts the protein folding homeostasis resulting in activation of the unfolded protein response (UPR). The UPR includes pathways that support adaptation to stress, and that are also implicated in promoting malignant features and therapy resistance ...
The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
Complete information for YWHAE gene (Protein Coding), Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Epsilon, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
The transcription factor C/EBPα is a critical mediator of myeloid differentiation and is often functionally impaired in acute myeloid leukemia. Recent studies have suggested that oncogenic FLT3 activity disrupts wild-type C/EBPα function via phosphorylation on serine 21 (S21). Despite the apparent r …
The CCAAT/Enhancer Binding Proteins (C/EBPs) are a family of leucine-zipper transcription factors that regulate physiological processes such as energy metabolism, inflammation, cell cycle, and the development and differentiation ...
Pharmacodynamic studies, including micro-ribonucleic acid (miRNA)-181 family and target gene expression, CCAAT/enhancer binding protein (C/EBP), alpha gene (CEBPA) expression, and genes involved in erythroid ...
Having analysed data with TRANSFAC system, we may assume that the disturbed attachment of such factors as (C/EBP(CCAAT enhancer binding protein) Hoxa-3,Sp1 (serine protease inhibitor) or GATA-1, (GATA nucleotide sequence) may have an impact on IGF-1 protein synthesis, but we did not observed any significant correlation between promoter P1 polymorphism and serum IGF-1 levels ...
Gentaur molecular products has all kinds of products like :search , Panomics \ C_EBPalpha _ C_EBPgamma EMSA Probe Set \ AY1274P for more molecular products just contact us
CCAAT-enhancer binding protein (C/EBP) β regulates insulin-like growth factor (IGF) 1 expression in porcine liver during prenatal and postnatal ...
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human CCAAT/Enhancer Binding Protein Beta (CEBPb) in samples from Tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species ...
Insulin can inhibit the stimulatory effect of glucocorticoid hormones on the transcription of genes coding for enzymes involved in glucose metabolism. We reported earlier that insulin inhibits the glucocorticoid-stimulated transcription of the gene coding for liver 6-phosphofructo-2-kinase (PFK-2). To elucidate the mechanism of these hormonal effects, we have studied the regulatory regions of the PFK-2 gene in transfection experiments. We found that both glucocorticoids and insulin act via the glucocorticoid response unit (GRU) located in the first intron. Footprinting experiments showed that the GRU binds not only the glucocorticoid receptor (GR), but also ubiquitous [nuclear factor I (NF-I)] and liver-enriched [hepatocyte nuclear factor (HNF)-3, HNF-6, CAAT/enhancer binding protein (C/EBP)] transcription factors. Site-directed mutational analysis of the GRU revealed that these factors modulate glucocorticoid action but that none of them seems to be individually involved in the inhibitory ...
TransAM C/EBP α/β Kits are DNA binding ELISAs that quantify the activated transcription factors using a method that is faster and more sensitive than gelshift, without radioactivity and gels.
Background The up-regulation of CCAAT/enhancer binding protein delta (CEBPD) has frequently been observed in macrophages in age-associated disorders, including rheumatoid arthritis (RA). However, the role of macrophage CEBPD in the pathogenesis of RA is unclear. Methodology and Principal Findings We found that the collagen-induced arthritis (CIA) score and the number of affected paws in Cebpd−/− mice were significantly decreased compared with the wild-type (WT) mice. The histological analysis revealed an attenuated CIA in Cebpd−/− mice, as shown by reduced pannus formation and greater integrity of joint architecture in affected paws of Cebpd−/− mice compared with WT mice. In addition, immunohistochemistry analysis revealed decreased pannus proliferation and angiogenesis in Cebpd−/− mice compared with WT mice. CEBPD activated in macrophages played a functional role in promoting the tube formation of endothelial cells and the migration and proliferation of synoviocytes. In vivo DNA
With SBIs line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time-our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid and ready-to-transduce pre-packaged lentivirus*. The pGreenFire1-C/EBP-α Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from C/EBP-α transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of C/EBP-α-responsive transcriptional elements, drives expression of dscGFP and luciferase in response to C/EBP-α activity. The result is the ability to quantitatively measure C/EBP-α activity by fluorescence and luciferase activity ...
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CCAAT/enhancer binding protein (C/EBP), epsilon, also known as CEBPE and CRP1, is a type of ccaat-enhancer-binding protein. CEBPE is its human gene and is pro-apoptotic. The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-δ. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with specific granule deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined. GRCh38: Ensembl release 89: ENSG00000092067 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000052435 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Entrez Gene: CEBPE CCAAT/enhancer binding protein (C/EBP), epsilon. Antonson P, Stellan B, Yamanaka R, ...
C/EBPs are a family of B-Zip transcription factors--TFs--involved in the regulation of differentiation in several tissues. The two most studied members--C/EBPα and C/EBPβ--play important roles in skin homeostasis and their ablation reveals cells with stem cells signatures. Much less is known about C/EBPδ which is highly expressed in the granular layer of interfollicular epidermis and is a direct target of p63, the master regular of multilayered epithelia. We identified C/EBPδ target genes in human primary keratinocytes by ChIP on chip and profiling of cells functionally inactivated with siRNA. Categorization suggests a role in differentiation and control of cell-cycle, particularly of G2/M genes. Among positively controlled targets are numerous genes involved in barrier function. Functional inactivation of C/EBPδ as well as overexpressions of two TF targets--MafB and SOX2--affect expression of markers of keratinocyte differentiation. We performed IHC on skin tumor tissue arrays expression ...
IL-6 induces PAI-1 mRNA and protein accumulation.13,19 Although several transcription factor binding sites were identified in PAI-1 promoter, no classic inflammatory response element was found. Because promoter activity was increased by IL-6, the IL-6-responsive region was explored. The deletion and site mutation of the region from −239 to −210 bp decreased ,80% of the IL-6-inducible promoter activity, indicating that the region is critical for response. A computer-based database analysis indicated there is a putative C/EBP binding site (−226 to −212 bp). The promoters of most IL-6-inducible acute-phase protein genes have been characterized with C/EBP binding motifs.11 Using competition experiments, EMSA supershift analysis, and DNase I footprinting analysis, a C/EBP motif on PAI-1 promoter was verified, and 3 members of C/EBP family including α, β, and δ were involved in the DNA-protein complex formation. C/EBPβ was involved in the formation of 3 complexes because single-copy ...
We have shown previously that a 500-bp region of the human insulin receptor promoter (−0.3 to −1.8 kb) was able to stimulate transcription from a heterologous thymidine kinase promoter in HepG2 hepatoma cells but not in HeLa fibroblasts. Footprint analysis localized the transcription factor binding sites to a 36-bp region at −1420. In this paper, we analyze the factors that recognize this element and show that it contains binding sites for the CAAT/enhancer binding protein C/EBP and nuclear factor 1 (NF-1). In addition we show that both C/EBPα and the C/EBPβ can transactivate the human insulin receptor promoter in a dose-dependent manner.. ...
Background: Tumor-associated macrophages (TAMs) are potent drivers of an immunosuppressive tumor microenvironment by reducing T cell recruitment and activation, promoting resistance to immune checkpoint inhibition. Experimental therapies blocking monocyte differentiation or eliminating TAMs show minimal anti-tumor activity. STAT6 and C/EBPβ have key roles in regulating the immunosuppressive state of TAMs however previous attempts to selectively target these transcription factors were unsuccessful. We have developed proprietary engineered exosome therapeutic candidates loaded with antisense oligos (ASOs) targeting STAT6 and C/EBPβ (exoASO). exoASO is designed to selectively deliver ASOs to TAMs and decrease STAT6 and C/EBPβ expression, leading to reprogramming of M2-like TAMs to a M1-like immune stimulatory phenotype.. Results: exoASO-STAT6 and exoASO-C/EBPβ induced dose-dependent knockdown (KD) of target genes in primary human M2 macrophages with greater potency (IC50) than free ASO. Gene ...
The gene encoding an important myeloid transcription factor is mutated in cells of acute myeloid leukemia (somatic mutation) and in patients with autosomal dominant familial acute myeloid leukemia (germline mutation).. ...
Compare C/EBP-delta ELISA Kits from Nordic BioSite from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.
Choi B.H., Park G.T., Rho H.M. (1999). Interaction of hepatitis B viral X protein and CCAAT/enhancer-binding protein alpha synergistically activates the hepatitis B viral enhancer II/pregenomic promoter.. J. Biol. Chem. 274: 2858 - 2865. PubMed DOI:10.1074/jbc.274.5.2858 ...
Multilevel issues of place and access confront rural populations and intensify disparities in cancer risk factors, incidence, mortality, and other outcomes. As rural cancer control is a national priority, CEBP will publish this Rural Cancer Control Focus Issue in advance of the AACR Annual Meeting and an NCI-sponsored conference in May 2018.. For more information, email [email protected] ...
Loss of RUNX1/ETO Triggers C/EBPα-Driven Reorganization of the Leukemic Transcriptional Network(A) RUNX1/ETO and CEBPA mRNA expression levels in Kasumi-1 cells
TY - JOUR. T1 - Differential expression of exons 1a and 1c in mRNAs for sterol regulatory element binding protein-1 in human and mouse organs and cultured cells. AU - Shimomura, Iichiro. AU - Shimano, Hitoshi. AU - Horton, Jay D.. AU - Goldstein, Joseph L.. AU - Brown, Michael S.. PY - 1997/3/1. Y1 - 1997/3/1. N2 - The 5 end of the mRNA-encoding sterol regulatory element binding protein-1 (SREBP-1) exists in two forms, designated 1a and 1c. The divergence results from the use of two transcription start sites that produce two separate 5 exons, each of which is spliced to a common exon 2. Here we show that the ratio of SREBP-1c to 1a transcripts varies markedly among organs of the adult mouse. At one extreme is the liver, in which the 1c transcript predominates by a 9:1 ratio. High 1c:1a ratios are also found in mouse adrenal gland and adipose tissue and in human liver and adrenal gland. At the other extreme is the spleen, which shows a reversed 1c:1a ratio (1:10). In five different lines of ...
In vitro, the transcription factor sterol regulatory element binding protein-1c (SREBP-1c) mimics the positive effects of insulin on hepatic genes involved in glucose utilization, such as glucokinase (GK) and enzymes of the lipogenic pathway, suggesting that it is a key factor in the control of hepatic glucose metabolism. Decreased glucose utilization and increased glucose production by the liver play an important role in the development of the hyperglycemia in diabetic states. We thus reasoned that if SREBP-1c is indeed a mediator of hepatic insulin action, a hepatic targeted overexpression of SREBP-1c should greatly improve glucose homeostasis in diabetic mice. This was achieved by injecting streptozotocin-induced diabetic mice with a recombinant adenovirus containing the cDNA of the mature, transcriptionally active form of SREBP-1c. We show here that overexpressing SREBP-1c specifically in the liver of diabetic mice induces GK and lipogenic enzyme gene expression and represses the expression of
TY - JOUR. T1 - SREBP cleavage-activating protein (SCAP) is required for increased lipid synthesis in liver induced by cholesterol deprivation and insulin elevation. AU - Matsuda, Morihiro. AU - Korn, Bobby S.. AU - Hammer, Robert E.. AU - Moon, Young Ah. AU - Komuro, Ryutaro. AU - Horton, Jay D.. AU - Goldstein, Joseph L.. AU - Brown, Michael S.. AU - Shimomura, Iichiro. PY - 2001/5/15. Y1 - 2001/5/15. N2 - In liver, the synthesis of cholesterol and fatty acids increases in response to cholesterol deprivation and insulin elevation, respectively. This regulatory mechanism underlies the adaptation to cholesterol synthesis inhibitors (statins) and high calorie diets (insulin). In nonhepatic cells, lipid synthesis is controlled by sterol regulatory element-binding proteins (SREBPs), membrane-bound transcription factors whose active domains are released proteolytically to enter the nucleus and activate genes involved in the synthesis and uptake of cholesterol and fatty acids. SCAP (SREBP ...
Neutrophil-specific granule deficiency (SGD) is a rare disorder characterized by recurrent pyogenic infections, defective neutrophil chemotaxis and bactericidal activity, and lack of neutrophil secondary granule proteins. It has been linked to a defect in the transcription factor CCAAT/enhancer binding protein (CEBP) epsilon. Recently, loss-of-function mutations in SMARCD2 were identified from SGD patients. SMARCD2 is chromatin-remodeling factor, that interacts with CEBP epsilon ...
Amelogenin gene expression is spatiotemporally regulated during enamel biomineralization. Studies show that C/EBP alpha is a transactivator of the mouse amelogenin gene acting at the C/EBP alpha cis-element located in the -70/+52 minimal promoter that also contains a reversed CCAAT box (-58/-54). Similar to the C/EBP alpha binding site, this CCAAT box is required for the basal promoter activity. Electrophoretic mobility shift assays demonstrate that NF-Y is directly bound to this reversed CCAAT box. Co-transfection of C/EBP alpha and NF-Y synergistically increases the promoter activity. Protein-protein interactions between C/EBP alpha with NF-Y are identified by a co-immunoprecipitation analysis.; A protein/DNA array technique is utilized to identify a transcriptional factor named YY1. YY1 represses both the basal amelogenin promoter activity and C/EBP alpha-mediated transactivation. Furthermore, YY1 repression is independent of its DNA binding capacity.; C/EBP alpha contains four highly ...
Rationale: Elevated levels of C/EBP homologous protein (CHOP), a member of the C/EBP transcription factor family, in advanced atherosclerotic plaques is reported to be associated with atherosclerotic plaque rupture in humans. However, the molecular mechanism by which CHOP accumulation occurs is poorly defined. Objective: The aim of this study was to investigate if (1) macrophage AMP-activated kinase (AMPK) regulates cellular CHOP accumulation and (2) whole-body Ampk deletion leads to neointimal disruption. Methods and Results: In isolated or cultured macrophages, Ampkα1 deletion markedly increased apoptosis and CHOP, whereas pharmacological activation of AMPK dramatically reduced CHOP protein level via promoting CHOP degradation by proteasome. In addition, co-transfection of Chop-specific siRNA, but not control siRNA, markedly reduced apoptosis in macrophages transfected with Ampkα1-specific siRNA. Mechanistically, AMPKα1 was found to co-immunoprecipitate with CHOP and phosphorylate CHOP at ...
CCAAT/enhancer binding protein zeta (mouse, aa620-633) Antibody (internal region), Peptide-affinity purified goat antibody validated in WB, E (AF3888a), Abgent
THOC5 is a nuclear/cytoplasmic protein member of the spliceosome complex which potentiates C/EBP expression in adipocyte differentiation. As C/EBP family members are important regulators of myelopoiesis and THOC5 is highly expressed in neutrophil/macrophage progenitor cells we assessed the role of THOC5 in cytokine-stimulated monocytic development. M-CSF stimulated maturation of the NFS60 cell line was associated with enhanced THOC5 expression and phosphorylation. THOC5 was also shown to form a complex with C/EBPbeta. Ectopic expression of THOC5 mimicked M-CSF mediated cell maturation and enhanced protein expression of the myeloid transcription factors C/EBPbeta, C/EBPalpha, Pu-1 and also GAB2 (a PI-3 Kinase and macrophage development regulator). Increased THOC5 expression also mimicked M-CSF stimulated increases in the lipid second messenger PtdInsP(3). Inhibition of THOC5-induced increases in PtdInsP(3) levels abrogated the elevated levels of C/EBPbeta. Thus THOC5 expression can potentiate ...
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DNA methylation has been implicated in the pathogenesis of chronic pain. However, the specific genes that are regulated by DNA methylation under neuropathic pain condition remain largely unknown. Here we investigated how chemokine receptor CXCR3 is regulated by DNA methylation and its contribution to neuropathic pain induced by spinal nerve ligation (SNL) in mice. SNL increased Cxcr3 mRNA and protein expression in the neurons of spinal cord. Meanwhile, the CpG island in the Cxcr3 gene promoter region was demethylated, and the expression of DNA methyltransferase 3b (DNMT3b) was decreased. SNL also increased the binding of CCAAT/enhancer binding protein α (C/EBPα) with Cxcr3 promoter and decreased the binding of DNMT3b with Cxcr3 promoter in the spinal cord. C/EBPα expression was increased in spinal neurons after SNL, and inhibition of C/EBPα by intrathecal siRNA attenuated SNL-induced pain hypersensitivity and reduced Cxcr3 expression. Furthermore, SNL-induced mechanical allodynia and heat ...
CCAAT/enhancer-binding protein δ (CEBPD) is expressed in hypoxic kidney tubular cells in vivo. (a) Mice were exposed to 8% O2 for 6 h using a hypoxia chamber
A recent paper in Nature Medicine showed that Down syndrome brains have reduced expression of Sorting nexin 27 (SNX27) and CCAAT/enhancer binding protein beta (C/EBP beta) and identified C/EBP beta as a transcription factor for SNX27. Down syndrome results in overexpression of miR-155, a chromosome 21-encoded microRNA that negatively regulates C/EBP beta, thereby reducing SNX27 expression. SNX27 is a brain-enriched […]. ...
TY - JOUR. T1 - Inhibition of LPS-induced C/EBPδ by trichostatin A has a positive effect on LPS-induced cyclooxygenase 2 expression in RAW264.7 cells. AU - Liu, Yi Wen. AU - Wang, Shao An. AU - Hsu, Tsung Yi. AU - Chen, Tsu An. AU - Chang, Wen Chang. AU - Hung, Jan Jong. PY - 2010/8/15. Y1 - 2010/8/15. N2 - Cyclooxygenase 2 (COX-2) is an important inflammatory factor. Previous studies have indicated that COX-2 is induced with lipopolysaccharide (LPS) treatment. Here, we found that an inhibitor of histone deacetylase (HDAC), trichostatin A (TSA), cannot repress LPS-induced COX-2 but it increased the COX-2 level in RAW264.7 cells. We found no significant difference in NF-κB activation and ERK1/2 phosphorylation, but LPS-induced C/EBPδ expression was completely abolished after TSA treatment of LPS-treated cells. Interesting, reporter assay of C/EBPδ promoter revealed that Sp1-binding site is important. Although there was no alteration in c-Jun levels, but the phosphorylation of c-Jun at its ...
The transcription factor CCAAT-enhancer-binding protein alpha (C/EBPα) is a master regulator of granulopoiesis and regulates the switch between proliferating, uncommitted progenitors and cell-cycle-arrested, differentiated myeloid cells. Usage of two alternative translation initiation sites in the CEBPA mRNA results in expression of a full-length C/EBPα protein p42 (42 kDa) and a shorter p30 isoform (30 kDa). CEBPA mutations are found in 9-15% of Acute Myeloid Leukemia (AML) patients. N-terminal frameshift mutations in the CEBPA gene lead to selective ablation of p42 expression, while C-terminal mutations disrupt the dimerization and DNA-binding ability of C/EBPα. AML patients harbor either mono- or biallelic CEBPA mutations (CEBPAmo or CEBPAbi) and both genotypes are frequently associated with concurrent mutations in other genes. The most commonly co-occurring mutations in both groups are loss-of-function mutations in the methylcytosine dioxygenase TET2 (44.4% in CEBPAmo / 34.8% in CEBPAbi). ...
Sterol regulatory element-binding protein 1c (SREBP-1c) is a central regulator of lipogenesis whose activity is controlled by proteolytic cleavage. The metabolic factors that affect its processing are incompletely understood. Here, we show that dynamic changes in the acyl chain composition of ER phospholipids affect SREBP-1c maturation in physiology and disease. The abundance of polyunsaturated phosphatidylcholine in liver ER is selectively increased in response to feeding and in the setting of obesity-linked insulin resistance. Exogenous delivery of polyunsaturated phosphatidylcholine to ER accelerated SREBP-1c processing through a mechanism that required an intact SREBP cleavage-activating protein (SCAP) pathway. Furthermore, induction of the phospholipid-remodeling enzyme LPCAT3 in response to liver X receptor (LXR) activation promoted SREBP-1c processing by driving the incorporation of polyunsaturated fatty acids into ER. Conversely, LPCAT3 deficiency increased membrane saturation, reduced ...
Sterol regulatory element-binding protein 1c (SREBP-1c) is a central regulator of lipogenesis whose activity is controlled by proteolytic cleavage. The metabolic factors that affect its processing are incompletely understood. Here, we show that dynamic changes in the acyl chain composition of ER phospholipids affect SREBP-1c maturation in physiology and disease. The abundance of polyunsaturated phosphatidylcholine in liver ER is selectively increased in response to feeding and in the setting of obesity-linked insulin resistance. Exogenous delivery of polyunsaturated phosphatidylcholine to ER accelerated SREBP-1c processing through a mechanism that required an intact SREBP cleavage-activating protein (SCAP) pathway. Furthermore, induction of the phospholipid-remodeling enzyme LPCAT3 in response to liver X receptor (LXR) activation promoted SREBP-1c processing by driving the incorporation of polyunsaturated fatty acids into ER. Conversely, LPCAT3 deficiency increased membrane saturation, reduced ...
Sterol regulatory element-binding protein 1c (SREBP-1c) is a central regulator of lipogenesis whose activity is controlled by proteolytic cleavage. The metabolic factors that affect its processing are incompletely understood. Here, we show that dynamic changes in the acyl chain composition of ER phospholipids affect SREBP-1c maturation in physiology and disease. The abundance of polyunsaturated phosphatidylcholine in liver ER is selectively increased in response to feeding and in the setting of obesity-linked insulin resistance. Exogenous delivery of polyunsaturated phosphatidylcholine to ER accelerated SREBP-1c processing through a mechanism that required an intact SREBP cleavage-activating protein (SCAP) pathway. Furthermore, induction of the phospholipid-remodeling enzyme LPCAT3 in response to liver X receptor (LXR) activation promoted SREBP-1c processing by driving the incorporation of polyunsaturated fatty acids into ER. Conversely, LPCAT3 deficiency increased membrane saturation, reduced ...
Here, we investigated the mechanisms by which PPARδ agonists control expression of 14-3-3ε, a key antiinflammatory protein in endothelial cells.12 Our data not only provide evidence that PPARδ modulates expression of YWHAE gene and 14-3-3ε protein under resting conditions but also demonstrate that this nuclear receptor upregulates 14-3-3ε expression by targeting transcription via a PPRE-independent pathway involving colocalization of C/EBPβ and PPARδ on YWHAE promoter. Several lines of evidence support these conclusions. First, PPARδ agonists regulated YWHAE promoter activity in a concentration- and time-dependent manner. Concordantly, YWHAE promoter was upregulated by PPARδ overexpression, whereas specific PPARγ and PPARα ligands had no effect on YWHAE promoter under our experimental conditions. Second, PPARδ activation increased 14-3-3ε mRNA and protein expression in both primary and spontaneously transformed endothelial cell lines, whereas PPARδ knockdown depressed basal and ...
We recently discovered that induction from the anti-inflammatory gene by cyclic AMP occurs through book cyclic AMP-dependent proteins kinase-independent systems involving activation of CCAAT/enhancer-binding proteins (C/EBP) transcription elements, notably C/EBP, from the cyclic AMP GEF EPAC1 as well as the ...
TY - CONF. T1 - A transcription factor complex at the beta-globin CCAAT site involving members of the C/EBP family of B-zip protein may be involved in haemoglobin switching. AU - Gordon, Chris. AU - Perkins, Andrew C. PY - 1999. Y1 - 1999. M3 - Abstract. SP - 1006. EP - 1006. T2 - 11th Lorne Cancer Conference. Y2 - 11 February 1999 through 14 February 1999. ER - ...
TY - JOUR. T1 - Regenerating livers of old rats contain high levels of C/EBPα that correlate with altered expression of cell cycle associated proteins. AU - Timchenko, Nikolai A.. AU - Wilde, Margaret. AU - Kosai, Ken Lchiro. AU - Heydari, Ahmed. AU - Bilyeu, Timothy A.. AU - Finegold, Milton J.. AU - Mohamedali, Khalid. AU - Richardson, Arlan. AU - Darlington, Gretchen J.. N1 - Funding Information: We thank W.Harper, S.Elledge and E.Harlow for cp36 antibodies and Dr J.Albrecht for His-C-p21. We thank K.Faraj for excellent assistance in the preparation of the manuscript. This work was supported by NIH grants DK45285 (G.J.D.), AG13663 (G.J.D.), AG00765-01 (N.A.T.) and GM55188-01 (N.A.T.), by AFAR grant A 97161, by The Moran Foundation and by the Estate of Evelyn Lucille Hansen.. PY - 1998/7/1. Y1 - 1998/7/1. N2 - The nuclear transcription factor, CCAAT/enhancer binding protein α (C/EBPα) is expressed at high levels in the liver and inhibits growth in cultured cells. We have tested the ...
One month following graft injection, no significant difference was noted between M2-supplemented (105±7.0 mm3) and control graft volumes (72±22 mm3). By three months post-injection, M2-supplemented grafts remained stable while controls experienced further volume loss (103±8 mm3 vs. 39.4±15 mm3, p=0.015). Presence of M2 macrophages in the supplemented grafts was confirmed by flow cytometry. M2-supplemented grafts demonstrated a 157% increase in vascular density compared to controls (p,0.05). Induction of adipogenic C/EBPα gene expression was observed when M2 supernatants were added to SVF containing ASC ...
The transcription factor C/EBP? is required for regulation of the balance between differentiation and proliferation during the early stages of myelopoiesis. The...
Liver is a unique tissue which is able to regenerate in response to partial hepatectomy (PH) and after injury. My laboratory investigates the role of transcript...
It is a member of the CCAAT/enhancer-binding protein (C/EBP) family of DNA-binding transcription factors. The protein functions ... Qiao D, Im E, Qi W, Martinez JD (June 2002). "Activator protein-1 and CCAAT/enhancer-binding protein mediated GADD153 ... Ramji DP, Foka P (August 2002). "CCAAT/enhancer-binding proteins: structure, function and regulation". The Biochemical Journal ... "Physical and functional association between GADD153 and CCAAT/enhancer-binding protein beta during cellular stress". The ...
Another aspect of the CCAAT binding motif is the CCAAT/enhancer binding proteins (C/EBPs). They are a group of transcription ... Protein specific binding is required for the CCAAT box activation. These proteins are known as CCAAT box binding proteins/CCAAT ... Ramji, Dpiak P.; Foka, Pelagia (10 May 2002). "Review Article: CCAAT/enhancer-binding proteins: structure, function and ... which are highly conserved and bind to the CCAAT motif. While research on these binding proteins is relatively recent, their ...
CCAAT/enhancer-binding protein alpha is a protein encoded by the CEBPA gene in humans. CCAAT/enhancer-binding protein alpha is ... For details on the CCAAT structural motif in gene enhancers and on CCAAT/Enhancer Binding Proteins see the specific page. The ... One category of mutations prevent CCAAT/enhancer-binding protein alpha DNA binding by altering its COOH-terminal basic leucine ... "Entrez Gene: CEBPA CCAAT/enhancer binding protein (C/EBP), alpha". Ohlsson E, Schuster MB, Hasemann M, Porse BT (Apr 2016). " ...
CCAAT/enhancer-binding protein beta is a protein that in humans is encoded by the CEBPB gene. The protein encoded by this ... "Entrez Gene: CEBPB CCAAT/enhancer binding protein (C/EBP), beta". Ruffell D, Mourkioti F, Gambardella A, Kirstetter P, Lopez RG ... Chen GK, Sale S, Tan T, Ermoian RP, Sikic BI (April 2004). "CCAAT/enhancer-binding protein beta (nuclear factor for interleukin ... Ccaat-enhancer-binding proteins GRCh38: Ensembl release 89: ENSG00000172216 - Ensembl, May 2017 GRCm38: Ensembl release 89: ...
CCAAT/enhancer-binding protein delta is a protein that in humans is encoded by the CEBPD gene. The protein encoded by this ... "CCAAT/enhancer-binding protein family members recruit the coactivator CREB-binding protein and trigger its phosphorylation". ... "Entrez Gene: CEBPD CCAAT/enhancer binding protein (C/EBP), delta". Gery S, Tanosaki S, Hofmann WK, Koppel A, Koeffler HP (Feb ... Li R, Strohmeyer R, Liang Z, Lue LF, Rogers J (Sep 2004). "CCAAT/enhancer binding protein delta (C/EBPdelta) expression and ...
CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. Ccaat-enhancer-binding proteins ... CCAAT/enhancer-binding protein gamma is a protein that in humans is encoded by the CEBPG gene. The C/EBP family of ... "Entrez Gene: CEBPG CCAAT/enhancer binding protein (C/EBP), gamma". Nishizawa M, Nagata S (1992). "cDNA clones encoding leucine- ... Involvement of C/enhancer-binding protein transcription factors and their possible interaction with an NF-IL-4 site". J. ...
CCAAT/enhancer binding protein (C/EBP), epsilon, also known as CEBPE and CRP1, is a type of ccaat-enhancer-binding protein. ... "Entrez Gene: CEBPE CCAAT/enhancer binding protein (C/EBP), epsilon". Antonson P, Stellan B, Yamanaka R, Xanthopoulos KG (Jul ... Antonson P, Stellan B, Yamanaka R, Xanthopoulos KG (Jul 1996). "A novel human CCAAT/enhancer binding protein gene, C/EBPepsilon ... Kim J, Cantwell CA, Johnson PF, Pfarr CM, Williams SC (Oct 2002). "Transcriptional activity of CCAAT/enhancer-binding proteins ...
"Molecular Mechanism of CCAAT-Enhancer Binding Protein Recruitment by the TRIB1 Pseudokinase". Structure. 23 (11): 2111-21. doi: ... Tribbles proteins function as scaffold proteins, which bind their substrates to localize them to or from their function ... It exerts its biological functions through binding to signalling proteins of the MAPKK level of the MAPK pathway, therefore ... The protein's primary structure contains a PEST region, indicative of proteins that are highly susceptible to degradation in ...
"G9a-mediated lysine methylation alters the function of CCAAT/enhancer-binding protein-beta". The Journal of Biological ... Nevertheless, increasing evidence suggests methylation of non-histone proteins may influence protein stability, protein-protein ... which is involved in protein-protein interactions. The ankyrin repeat domain also contains H3K9me1 and H3K9me2 binding sites. ... Euchromatic histone-lysine N-methyltransferase 1, also known as G9a-like protein (GLP), is a protein that in humans is encoded ...
Marchildon, François (2012). "CCAAT/Enhancer Binding Protein Beta is Expressed in Satellite Cells and Controls Myogenesis". ... Moreover, both quiescent and activated human satellite cells can be identified by the membrane-bound neural cell adhesion ... There is some research indicating that satellite cells are negatively regulated by a protein called myostatin. Increased levels ... Activated satellite cells also begin expressing muscle-specific filament proteins such as desmin as they differentiate. The ...
Hanlon M, Sealy L (May 1999). "Ras regulates the association of serum response factor and CCAAT/enhancer-binding protein beta ... This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the ... Serum response factor has been shown to interact with: ASCC3, ATF6, CEBPB, CREB-binding protein, ELK4, GATA4, GTF2F1, GTF2I, ... "A multifunctional DNA-binding protein that promotes the formation of serum response factor/homeodomain complexes: identity to ...
"Different regulation of the LXRalpha promoter activity by isoforms of CCAAT/enhancer-binding proteins". Biochemical and ... Liver X receptor alpha (LXR-alpha) is a nuclear receptor protein that in humans is encoded by the NR1H3 gene (nuclear receptor ... The liver X receptors, LXRα (this protein) and LXRβ, form a subfamily of the nuclear receptor superfamily and are key ... Miyata KS, McCaw SE, Meertens LM, Patel HV, Rachubinski RA, Capone JP (Nov 1998). "Receptor-interacting protein 140 interacts ...
"Capsaicin induces CYP3A4 expression via pregnane X receptor and CCAAT/enhancer-binding protein β activation". Molecular ... Due to membrane-bound CYP3A4's natural propensity to conglomerate, it has historically been difficult to study drug binding in ... which binds to the XREM region of the CYP3A4 gene. XREM is a regulatory region of the CYP3A4 gene, and binding causes a ... Ligand binding increases when in the presence of CYP3A4 ligands, such as in the presence of aflatoxin B1, M1, and G1. Indeed, ...
Boruk M, Savory JG, Haché RJ (Nov 1998). "AF-2-dependent potentiation of CCAAT enhancer binding protein beta-mediated ... "Entrez Gene: DBI diazepam binding inhibitor (GABA receptor modulator, acyl-Coenzyme A binding protein)". Kos M, Reid G, Denger ... Chan SW, Hong W (Jul 2001). "Retinoblastoma-binding protein 2 (Rbp2) potentiates nuclear hormone receptor-mediated ... CREB Binding Protein Interaction Interface and Its Importance for the Function of SRC1". Mol. Cell. Biol. 21 (1): 39-50. doi: ...
March 2001). "Dominant-negative mutations of CEBPA, encoding CCAAT/enhancer binding protein-alpha (C/EBPalpha), in acute ... an aggregate of multiple copies of the same protein, otherwise known as a homomultimeric protein or homooligomeric protein. In ... For example, in humans the Hb gene locus is responsible for the Beta-chain protein (HBB) that is one of the two globin proteins ... A mutation that leads to a mutant protein that disrupts the activity of the wild-type protein in the multimer is a dominant- ...
C/EBP-beta (bahasa Inggris: CCAAT/enhancer-binding protein beta) adalah faktor transkripsi bZIP yang memiliki berkas genetik ... "CEBPB CCAAT/enhancer binding protein (C/EBP), beta [ Homo sapiens ]". Entrez Gene. Diakses tanggal 2010-10-27.. ... C/EBP-beta dapat membentuk kompleks heterdimer dengan protein sejenis seperti C/EBP-alfa, C/EBP-delta, C/EBP-gamma. ...
March 2001). "Dominant-negative mutations of CEBPA, encoding CCAAT/enhancer binding protein-alpha (C/EBPalpha), in acute ... an aggregate of multiple copies of the same protein, otherwise known as a homomultimeric protein or homooligomeric protein. In ... For example, in humans the Hb gene locus is responsible for the Beta-chain protein (HBB) that is one of the two globin proteins ... In fact, the first study reporting a mutant protein inhibiting the normal function of a wild-type protein in a mixed multimer ...
... cholesterol acyltransferase1 gene expression via MAP kinases and CCAAT/enhancer-binding protein α.". Journal of Cellular ... First, it is activated by potassium ions binding near the CoA binding site and the catalytic site. This binding causes a ... There are two functional sections of this protein, TMD7 and TMD8; one side is involved in substrate binding and catalysis, ... The gene also may have a binding site for the transcription factor Sp1, and has sequences resembling the binding sites of ...
... and duodenal homeobox gene 1 induces hepatic dedifferentiation by suppressing the expression of CCAAT/enhancer-binding protein ... microRNAs and a variety of other methods including using proteins and plasmids; one example is the non-viral delivery of ... markers of the target cell type and the absence of donor cell markers which can be accomplished using green fluorescent protein ...
Jurado LA, Song S, Roesler WJ, Park EA (2002). "Conserved amino acids within CCAAT enhancer-binding proteins (C/EBP(alpha) and ... A mitochondrial isozyme of the encoded protein also has been characterized. Click on genes, proteins and metabolites below to ... Wilson HL, McFie PJ, Roesler WJ (2003). "Different transcription factor binding arrays modulate the cAMP responsivity of the ... 2002). "Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site". J. Mol. Biol. ...
"Transcriptional regulation of human CYP3A4 basal expression by CCAAT enhancer-binding protein alpha and hepatocyte nuclear ... "Cloning and characterization of seven human forkhead proteins: binding site specificity and DNA bending". The EMBO Journal. 13 ... Forkhead box I1 is a protein that in humans is encoded by the FOXI1 gene. This gene belongs to the forkhead family of ... FOX proteins GRCh38: Ensembl release 89: ENSG00000168269 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000047861 - ...
2003). "Transcriptional regulation of human CYP3A4 basal expression by CCAAT enhancer-binding protein alpha and hepatocyte ... is a protein that in humans is encoded by the FOXA3 gene. HNF-3G is a member of the forkheadclass of DNA-binding proteins. ... 2009). "Differential binding and co-binding pattern of FOXA1 and FOXA3 and their relation to H3K4me3 in HepG2 cells revealed by ... Hepatocyte nuclear factor 3-gamma (HNF-3G), also known as forkhead box protein A3 (FOXA3) or transcription factor 3G (TCF-3G) ...
IL-6 activates the CCAAT enhancer-binding protein transcription factors which activate MDR1 gene expression (see Alteration of ... This transporter protein is encoded by the MDR1 gene and is also called the ATP-binding cassette (ABC) protein. MDR1 has ... MDR1 is activated through NF-κB, a protein complex which acts as a transcription factor. In the rat, an NF-κB binding site is ... These point mutations enhance autophosphorylation of the BCR-ABL protein, resulting in the stabilization of the ATP-binding ...
"AU-rich element-binding protein negatively regulates CCAAT enhancer-binding protein mRNA stability during long-term synaptic ... "Overexpression of and RNA interference with the CCAAT enhancer-binding protein on long-term facilitation of Aplysia sensory to ... Lee JA, Kim H, Lee YS, Kaang BK (2003). "Overexpression and RNA interference of Ap-cyclic AMP-response element binding protein- ... transcription requires the protein kinase-A-mediated phosphorylation of the cAMP-response element-binding protein(CREB). ...
... of a co-repressor that inhibits the transcriptional and growth-arrest activities of CCAAT/enhancer-binding protein alpha". J. ... 2000). "Protein-interaction modules that organize nuclear function: FF domains of CA150 bind the phosphoCTD of RNA polymerase ... 2004). "A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease". Mol. Cell. ... This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein ...
Eaton EM, Sealy L (Aug 2003). "Modification of CCAAT/enhancer-binding protein-beta by the small ubiquitin-like modifier (SUMO) ... Kim J, Cantwell CA, Johnson PF, Pfarr CM, Williams SC (Oct 2002). "Transcriptional activity of CCAAT/enhancer-binding proteins ... It is a ubiquitin-like protein and functions in a manner similar to ubiquitin in that it is bound to target proteins as part of ... Small ubiquitin-related modifier 2 is a protein that in humans is encoded by the SUMO2 gene. This gene encodes a protein that ...
"CEBPE CCAAT/enhancer binding protein epsilon [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2016-05-09 ... The C8orf48 protein is predicted to be a nuclear protein particularly located in the nuclear lamina. This protein does not ... These transcription factors include MAX binding protein and Estrogen-related receptor alpha (secondary DNA binding preference) ... "Characterization of the DNA-binding properties of the myeloid zinc finger protein MZF1: two independent DNA-binding domains ...
Transcription factors, peroxis proliferator-activated receptor γ (PPARγ) and CCAAT enhancer-binding proteins (C/EBPs) are main ... cAMP-responsive element binding protein promotes differentiation, while the activation of PPARγ and C/EBPα is also responsive ... T-cell factor/lymphoid enhancer-binding factor (TCF/LEF), GATA2/3, retinoic acid receptor α, and SMAD6/7 don't affect the ... Adipocyte determination and differentiation factor 1 (ADD1) and sterol regulatory element binding protein 1 (SREBP1) can ...
Eaton EM, Sealy L (August 2003). "Modification of CCAAT/enhancer-binding protein-beta by the small ubiquitin-like modifier ( ... "A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy ... Dobreva G, Dambacher J, Grosschedl R (December 2003). "SUMO modification of a novel MAR-binding protein, SATB2, modulates ... Small ubiquitin-related modifier 3 is a protein that in humans is encoded by the SUMO3 gene. SUMO proteins, such as SUMO3, and ...
Barakat et al (2015). CCAAT/Enhancer binding protein β controls androgen-deprivation-induced senescence in prostate cancer ...
CEBPA, C/EBP-alpha, CEBP, CCAAT/enhancer binding protein alpha, CCAAT enhancer binding protein alpha. ... Choi B.H., Park G.T., Rho H.M. (1999). Interaction of hepatitis B viral X protein and CCAAT/enhancer-binding protein alpha ... CEBPA (англ. CCAAT/enhancer binding protein alpha) - білок, який кодується однойменним геном, розташованим у людей на короткому ... Muller C., Calkhoven C.F., Sha X., Leutz A. (2004). The CCAAT enhancer-binding protein alpha (C/EBPalpha) requires a SWI/SNF ...
In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... Promoters represent critical elements that can work in concert with other regulatory regions (enhancers, silencers, boundary ... CCAAT boxes are common, as they are in many promoters that lack TATA boxes. In addition, the motifs NRF-1, GABPA, YY1, and ... which in turn are often brought to the promoter DNA by an activator protein's binding to its own DNA binding site nearby.. In ...
"CCAAT/enhancer-binding protein family members recruit the coactivator CREB-binding protein and trigger its phosphorylation". J ... Protein phosphorylation[edit]. Main article: Protein phosphorylation. Function[edit]. Reversible phosphorylation of proteins is ... multi-protein enzyme present in phagocytic cells, plays an important role in the regulation of protein-protein interactions in ... protein A phosphorylates protein B, and B phosphorylates C. However, in another signaling pathway, protein D phosphorylates A, ...
... although the consensus binding site for the TATA-binding protein (TBP) is TATAAAA, the TBP transcription factor can also bind ... DNA-binding domain (DBD), which attaches to specific sequences of DNA (enhancer or promoter. Necessary component for all ... I. constitutively active - present in all cells at all times - general transcription factors, Sp1, NF1, CCAAT ... TAD is domain of the transcription factor that binds other proteins such as transcription coregulators. Proteins containing ...
Sterneck began to study the functions of CCAAT-enhancer-binding proteins (C/EBP) transcription factors, including their roles ...
Boruk M, Savory JG, Haché RJ (November 1998). "AF-2-dependent potentiation of CCAAT enhancer binding protein beta-mediated ... the heat shock protein 70 (hsp70) and the protein FKBP4 (FK506-binding protein 4). The endogenous glucocorticoid hormone ... Hulkko SM, Wakui H, Zilliacus J (August 2000). "The pro-apoptotic protein death-associated protein 3 (DAP3) interacts with the ... DNA-binding domain (DBD) D - hinge region E - ligand-binding domain (LBD) F - C-terminal domain In the absence of hormone, the ...
"Regulation of the homeodomain CCAAT displacement/cut protein function by histone acetyltransferases p300/CREB-binding protein ( ... matrix attachment region upstream of the T cell receptor beta gene enhancer binds Cux/CDP and SATB1 and modulates enhancer- ... "Regulation of the homeodomain CCAAT displacement/cut protein function by histone acetyltransferases p300/CREB-binding protein ( ... The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It regulates gene expression, ...
CCAAT/enhancer-binding protein alpha), glucocorticoid receptors α and β, and p53. Expression of isoforms 1 and 2 has been ... these tyrosine motifs are bound by the SH2 domains of signaling proteins. Important binding partners for this region include ... allowing the CAS protein to function as a scaffold for other proteins including CRK proteins and C3G, a guanine nucleotide ... All CAS proteins except CASS4 contain a YDYVHL motif within this domain, which is an important binding site for the Src SH2 ...
An important example is that the proapoptotic protein CHOP (CCAAT/-enhancer-binding protein homologous protein), is upregulated ... exposed hydrophobic residues of the misfolded protein are bound by the protein glucose regulate protein 78 (Grp78), a heat ... "Heavy chain binding protein recognizes incompletely disulfide-bonded forms of vesicular stomatitis virus G protein". The ... By binding with the protein TRAF2, Ire1 activates a JNK signaling pathway, at which point human procaspase 4 is believed to ...
Transcription factor assessment indicates many potential TATA-binding protein and CCAAT-enhancer-binding proteins sites, along ... C1orf112 is predicted to interact with a diverse range of proteins, including multiple mitosis-associated proteins. C1orf112 is ... quality-controlled protein-protein association networks, made broadly accessible". Nucleic Acids Research. 45 (D1): D362-D368. ... Ligand binding sites are predicted by I-TASSER from positions 377 to 530 in Isoform X1. A leucine zipper motif is present in ...
Zuo Y, Qiang L, Farmer SR (March 2006). "Activation of CCAAT/enhancer-binding protein (C/EBP) alpha expression by C/EBP beta ... binding protein], PACT (protein activator of the interferon-induced protein kinase), the SMN complex, fragile X mental ... significantly inhibited adipogenesis and repressed induction of the master regulators PPARγ and CCAAT/enhancer-binding protein ... These proteins have three highly positively charged regions, termed AT hooks, that bind the minor groove of AT-rich DNA ...
"CCAAT/Enhancer-binding Protein Family Members Recruit the Coactivator CREB-binding Protein and Trigger Its Phosphorylation". ... PMID 12857754.CS1 maint: multiple names: authors list (link) Ramji D.P., Foka P. (2002). "CCAAT/enhancer-binding proteins: ... As suggested by the protein's name, glutamate-rich protein 4, the protein is most highly composed of glutamic acid amino acids ... "TATA-binding proteins". InterPro. Retrieved 6 May 2019. "SRY Gene". Genetics Home Reference. Vihervaara A, Sistonen L (2014). " ...
TFG-TEC binds to the proximal promoter region of the ENO3 gene. Click on genes, proteins and metabolites below to link to ... activator protein 1 and 2, CCAAT box transcription factor/nuclear factor I, and cyclic AMP. Unlike the other enolase genes, ... and two myocyte-specific enhancer-binding factor 1 boxes. Upstream of the first exon lies a TATA-like box and CpG-rich region, ... Peshavaria M, Hinks LJ, Day IN (Nov 1989). "Structure of human muscle (beta) enolase mRNA and protein deduced from a genomic ...
"A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy ... E3 SUMO-protein ligase PIAS4 is one of several protein inhibitor of activated STAT (PIAS) proteins. It is also known as protein ... Mothers against decapentaplegic homolog 7 and Lymphoid enhancer-binding factor 1. GRCh38: Ensembl release 89: ENSG00000105229 ... "Entrez Gene: PIAS4 Protein inhibitor of activated STAT, 4". Imoto, Seiyu; Sugiyama Kenji; Muromoto Ryuta; Sato Noriko; Yamamoto ...
... gene involved in regulation of fatty acid storage and glucose metabolism and members of the CCAAT/enhancer-binding protein (C/ ... This process is regulated by the nuclear receptor peroxisome proliferator-activated receptor γ(PPARγ), a protein regulating ... which are exported into the blood and bound to albumin, and glycerol, which is exported into the blood freely. There is ...
... is transcription factor CCAAT-enhancer binding protein α (C/EBPα). Mutations in C/EBPα are associated with acute myeloid ... "Ikaros DNA-binding proteins as integral components of B cell developmental-stage-specific regulatory circuits". Immunity. 26 (3 ... One of the key players in self-renewal and development of haematopoietic cells is stem cell factor (SCF), which binds to the c- ... Very important finding is, that different zinc fingers are involved in binding to different place in DNA and this is the reason ...
Ccaat/Enhancer Binding Protein Vertebrate TATA binding protein factor CCAAT binding factors Activator-, mediator- and TBP- ... factor 1 RXR heterodimer binding sites GATA binding factors Nuclear receptor subfamily 2 factors Octamer binding protein EGR/ ... proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be ... calcium ion binding, protein binding. Analysis of gene ontology information by BioGPS was able to produce a list of biological ...
CCAAT binding factors, or CCAAT enhancer binding proteins were found. The TMEM251 protein is 169 amino acids in length. The ... Transmembrane protein 251, also known as C14orf109 or UPF0694, is a protein that in humans is encoded by the TMEM251 gene. One ... No vertebrate TATA binding protein factors, RNA polymerase transcription factor II B, ... The first transcript variant encodes a shorter predicted protein, while the second transcript variant encodes a protein with a ...
... may refer to: CEBPA, a human gene that modulates leptin expression CCAAT-enhancer-binding proteins or C/EBPs Communication ...
"Stem-loop binding protein facilitates 3'-end formation by stabilizing U7 snRNP binding to histone pre-mRNA". Mol Cell Biol. 19 ... "U7 snRNA acts as a transcriptional regulator interacting with an inverted CCAAT sequence-binding transcription factor NF-Y". ... SMN2 pre-mRNA splicing corrected by a U7 snRNA derivative carrying a splicing enhancer sequence". Mol Ther. 15 (8): 1479-1486. ... "A novel zinc finger protein is associated with U7 snRNP and interacts with the stem-loop binding protein in the histone pre- ...
For example, CCAAT/enhancer-binding protein β (C/EBPβ), an early adipogenic transcription factor, recruits and requires KMT2D ... KMT2C and KMT2D are required for the binding of H3K27 acetyltransferases CREB-binding protein (CBP) and/or p300 on enhancers, ... The protein selectively binds enhancer regions based on type of cell and stage of differentiation. During differentiation, ... The KMT2C and KMT2D proteins, rather than the KMT2C and KMT2D-mediated H3K4me1, control p300 recruitment to enhancers, enhancer ...
RAV Cdx protein family DNA-binding protein Inhibitor of DNA-binding protein Nuclear receptor, a class of ligand activated ... Transcription factors bind to either enhancer or promoter regions of DNA adjacent to the genes that they regulate. Depending on ... Heteromeric CCAAT factors 4.8.1 Family: Heteromeric CCAAT factors 4.9 Class: Grainyhead 4.9.1 Family: Grainyhead 4.10 Class: ... although the consensus binding site for the TATA-binding protein (TBP) is TATAAAA, the TBP transcription factor can also bind ...
Regulation of Id2 expression by CCAAT/enhancer binding protein beta.. Karaya K1, Mori S, Kimoto H, Shima Y, Tsuji Y, Kurooka H ... similar to the mice lacking the CCAAT enhancer binding protein (C/EBP) beta. Here, we show that Id2 is a direct target of C/ ... Oligonucleotide HpxA was used as a positive control for C/EBPβ binding. DNA-protein complexes were separated on a 6% ... which was achieved by using a system utilizing the fusion protein between C/EBPbeta and the ligand-binding domain of the human ...
"CCAAT/enhancer-binding protein family members recruit the coactivator CREB-binding protein and trigger its phosphorylation". ... CCAAT-enhancer-binding proteins (or C/EBPs) is a family of transcription factors composed of six members, named from C/EBPα to ... CCAAT/enhancer-binding proteins are often involved in growth arrest and differentiation, which has been interpreted to suggest ... The function of CCAAT/enhancer-binding proteins in cancer is thus clearly context dependent but largely tumor suppressive. ...
CCAAT/enhancer-binding protein delta; Short=C/EBP delta; AltName: ... RecName: Full=CCAAT/enhancer-binding protein delta; Short ... RefSeq protein See the reference protein sequence for CCAAT/enhancer-binding protein delta (NP_005186.2). ... RecName: Full=CCAAT/enhancer-binding protein delta; Short=C/EBP delta; AltName: Full=Nuclear factor NF-IL6-beta; Short=NF-IL6- ... HMDB and 5-AzadC Combination Reverses Tumor Suppressor CCAAT/Enhancer-Binding Protein Delta to Strengthen the Death of Liver ...
... The introduction to this article provides insufficient context for those unfamiliar with the ... CCAAT-enhancer-binding proteins (or C/EBPs) are a family of transcription factors that are composed of six members C/EBP α to C ... It uses material from the Wikipedia article "Ccaat-enhancer-binding_proteins". A list of authors is available in Wikipedia. ... Ramji, D. P. & Foka P., CCAAT/enhancer-binding proteins: structure, function and regulation, Biochem. J. 365:561-575 (2002). ...
Tamara Tchkonia, Nino Giorgadze, Tamar Pirtskhalava, Thomas Thomou, Matthew DePonte, Ada Koo, R. Armour Forse, Dharmaraj Chinnappan, Carmen Martin-Ruiz, Thomas von Zglinicki, James L. Kirkland ...
Compare CCAAT enhancer binding protein delta ELISA Kits from leading suppliers on Biocompare. View specifications, prices, ... CCAAT enhancer binding protein delta ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a widely used application for ... Your search returned 52 CCAAT enhancer binding protein delta ELISA ELISA Kit across 12 suppliers. ... Watch Webinar: How To Get Speed and Depth in your Host Cell Protein (HCP) Analysis ...
Compare Anti-CCAAT/enhancer binding protein epsilon Antibody Products from leading suppliers on Biocompare. View specifications ... Anti-CCAAT/enhancer binding protein epsilon Antibody Products. Anti-CCAAT/enhancer binding protein epsilon antibodies can be ... The CCAAT/enhancer binding protein epsilon protein is a reported synonym for the human gene CEBPE, encoding CCAAT enhancer ... Your search returned 241 CCAAT/enhancer binding protein epsilon Antibodies across 25 suppliers. ...
CCAAT/enhancer-binding protein family members recruit the coactivator CREB-binding protein and trigger its phosphorylation, J ... CCAAT-enhancer-binding proteins (or C/EBPs) are a family of transcription factors, composed of six members called C/EBP α to C/ ... 1. Ramji, D. P. & Foka P., CCAAT/enhancer-binding proteins: structure, function and regulation, Biochem. J. 365:561-575 (2002). ... C/EBP proteins interact with the CCAAT (cytidine-cytidine-adenosine-adenosine-thymidine) box motif which is present in several ...
... the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Required for the ... C/EBP are DNA-binding proteins that recognize two different motifs: ... CCAAT/enhancer-binding protein epsilonAdd BLAST. 281. Amino acid modifications. Feature key. Position(s). DescriptionActions. ... sp,P56261,CEBPE_RAT CCAAT/enhancer-binding protein epsilon OS=Rattus norvegicus GN=Cebpe PE=1 SV=2 ...
... the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers (PubMed:16397300). ... CCAAT/enhancer-binding protein deltaAdd BLAST. 268. Amino acid modifications. Feature key. Position(s). DescriptionActions. ... sp,P49716,CEBPD_HUMAN CCAAT/enhancer-binding protein delta OS=Homo sapiens OX=9606 GN=CEBPD PE=1 SV=2 ... identical protein binding Source: Ensembl. *RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: ...
CCAAT/enhancer-binding protein zeta is a protein that in humans is encoded by the CEBPZ gene.[1][2][3] ... Ccaat-enhancer-binding proteins. References. *↑ Lum LS, Sultzman LA, Kaufman RJ, Linzer DI, Wu BJ (Jan 1991). "A cloned human ... "Entrez Gene: CEBPZ CCAAT/enhancer binding protein zeta".. ,templatestyles src="Module:Citation/CS1/styles.css",,/templatestyles ... Retrieved from "https://www.wikidoc.org/index.php?title=CCAAT/enhancer_binding_protein_zeta&oldid=1412751" ...
Interleukin-6-dependent DNA-binding protein (IL6DBP); Nuclear factor for IL-6 expression (NF-IL6); Transcription factor 5 (TCF5 ... CCAAT-enhancer binding protein β (C/EBP-β); ... CCAAT-enhancer binding protein β (C/EBP-β); Interleukin-6- ... CCAAT/enhancer binding protein beta is expressed in satellite cells and controls myogenesis. Stem Cells. 2012;30:2619-30. https ... The nuclear factor for IL-6 expression (NF-IL6) was discovered as a member of the CCAAT-enhancer binding proteins (C/EBP) in ...
CCAAT/Enhancer Binding Protein and Nuclear Factor-Y Regulate Adiponectin Gene Expression in Adipose Tissue. ... CCAAT/Enhancer Binding Protein and Nuclear Factor-Y Regulate Adiponectin Gene Expression in Adipose Tissue ... CCAAT/Enhancer Binding Protein and Nuclear Factor-Y Regulate Adiponectin Gene Expression in Adipose Tissue ... CCAAT/Enhancer Binding Protein and Nuclear Factor-Y Regulate Adiponectin Gene Expression in Adipose Tissue ...
The transcription factor CCAAT/Enhancer-Binding Protein alpha (C/EBPa) coordinates proliferation arrest and differentiation of ... Der Transkriptionsfaktor CCAAT/Enhancer-Binding Protein alpha (C/EBPa) koordiniert Proliferationshemmung und Differenzierung ... Molecular mechanisms of gene activation and gene expression mediated by CCAAT/enhancer binding proteins. ... but by direct protein-protein interactions that abolish the binding of C/EBPa to DNA. This mechanism of transcriptional ...
... and noradrenaline induce the transcription factors CCAAT/enhancer binding protein (C/EBP)-beta and C/EBP delta in mouse ... and noradrenaline induce the transcription factors CCAAT/enhancer binding protein (C/EBP)-beta and C/EBP delta in mouse ... and noradrenaline induce the transcription factors CCAAT/enhancer binding protein (C/EBP)-beta and C/EBP delta in mouse ... and noradrenaline induce the transcription factors CCAAT/enhancer binding protein (C/EBP)-beta and C/EBP delta in mouse ...
CCAAT-enhancer binding protein: a component of a differentiation switch Message Subject. (Your Name) has forwarded a page to ... The CCAAT-enhancer binding protein (C/EBP) has now been found to promote the terminal differentiation of adipocytes. During the ... CCAAT-enhancer binding protein: a component of a differentiation switch. By RM Umek, AD Friedman, SL McKnight ... CCAAT-enhancer binding protein: a component of a differentiation switch. By RM Umek, AD Friedman, SL McKnight ...
... J ... cells of asthmatic patients have an impaired expression of CCAAT/enhancer binding protein (C/EBP) alpha, which is associated ... CCAAT-Enhancer-Binding Protein-alpha / biosynthesis* * CCAAT-Enhancer-Binding Protein-alpha / immunology ... and 4E binding protein. Results: Compared with healthy control subjects, BSM cells of asthmatic patients proliferate faster ( ...
What is CCAAT/enhancer-binding protein homologous protein? Meaning of CCAAT/enhancer-binding protein homologous protein medical ... What does CCAAT/enhancer-binding protein homologous protein mean? ... Looking for online definition of CCAAT/enhancer-binding protein ... homologous protein in the Medical Dictionary? CCAAT/enhancer-binding protein homologous protein explanation free. ... CCAAT/enhancer-binding protein homologous protein , definition of CCAAT/enhancer-binding protein homologous protein by Medical ...
CCAAT/enhancer-binding protein. CHOP. CCAAT/enhancer-binding protein homologous protein. dn. dominant-negative. DON. ... CCAAT/enhancer-binding protein homologous protein (CHOP) regulates osteoblast differentiation. Mol. Cell. Biol. 26: 6105-6116. ... CCAAT/enhancer-binding protein (C/EBP) homologous protein (CHOP)/growth arrest and DNA damage-inducible gene 153 are C/EBP ... CCAAT/enhancer-binding protein homologous protein (CHOP) is a crucial stress-responsive factor in various mucosal injuries, ...
Effect of hypoxia on the expression of CCAAT/enhancer-binding protein β and receptor activator of nuclear factor kappa-B ligand ... enhancer-binding protein-β (C/EBPβ) and the receptor activator of nuclear factor kappa-B ligand (RANKL) expressions in ... In this study, the role of hypoxia-inducible factor-1 (HIF-1) signaling in the regulation of CCAAT (cytosine-cytosine-adenosine ... monocyte chemoattractant protein-1, and macrophage colony-stimulating factor in the culture media. Treatment of the cells with ...
In vivo DNA binding assays and reporter assays showed that CEBPD up-regulated CCL20, CXCL1, IL23A and TNFAIP6 transcripts ... CCL20, IL23A, CXCL1 and TNFAIP6 contributed to the migration and proliferation of synoviocytes, and the latter two proteins ... Background The up-regulation of CCAAT/enhancer binding protein delta (CEBPD) has frequently been observed in macrophages in age ... through direct binding to their promoter regions. ...
Selective suppression of CCAAT/enhancer-binding protein β binding and cyclooxygenase-2 promoter activity by sodium salicylate ... CCAAT-enhancer-binding proteins (C/EBP) regulate the tissue specific activity of the CD11c intergrin gene promoter through ... The induction of cyclooxygenase-2 mRNA in macrophages is biphasic and requires both CCAAT enhancer-binding protein β (C/EBPβ) ... Dynamic regulation of cyclooxygenase-2 promoter activity by isoforms of CCAAT/enhancer-binding proteins. J. Biol. Chem. 277: ...
RasV12-Mediated Down-regulation of CCAAT/Enhancer Binding Protein β in Immortalized Fibroblasts Requires Loss of p19Arf and ... RasV12-Mediated Down-regulation of CCAAT/Enhancer Binding Protein β in Immortalized Fibroblasts Requires Loss of p19Arf and ... RasV12-Mediated Down-regulation of CCAAT/Enhancer Binding Protein β in Immortalized Fibroblasts Requires Loss of p19Arf and ... RasV12-Mediated Down-regulation of CCAAT/Enhancer Binding Protein β in Immortalized Fibroblasts Requires Loss of p19Arf and ...
Studies into the regulation of CCAAT Enhancer Binding Protein delta expression (C EBP) delta by cytokines ... Expression of APP genes is known to be regulated by the CCAAT Enhancer Binding Protein (C/EBP) family of transcription factors ... However, unlike endogenous C/EBP5 mRNA and protein expression, both of which were induced by IL-1, the activity of the human C/ ... is characterised by changes in levels of serum acute phase response proteins (APPs). These APPs are synthesised primarily by ...
... to TRAIL-induced apoptosis via CCAAT/enhancer-binding protein homologous protein-dependent DR5 up-regulation. ... Our results also indicate that an increase in CCAAT/enhancer binding protein homologous protein (CHOP) protein levels precedes ... to TRAIL-induced apoptosis via CCAAT/enhancer-binding protein homologous protein-dependent DR5 up-regulation.. Kim EH, Yoon MJ ... to TRAIL-induced apoptosis via CCAAT/enhancer-binding protein homologous protein-dependent DR5 up-regulation.. Posted on: 01/13 ...
10 CCAAT/enhancer-binding proteins (C/EBPs) binding motifs are identified in the promoter regions of most acute-phase protein ... containing a putative CCAAT/enhancer-binding protein (C/EBP) binding site was necessary. Point mutation in this site abolished ... Biological role of the CCAAT/enhancer-binding protein family of transcription factors. J Biol Chem. 1998; 273: 28545-28548. ... Interleukin-6 and Mevastatin Regulate Plasminogen Activator Inhibitor-1 Through CCAAT/Enhancer-Binding Protein-δ. Jie Dong, ...
Deficiency of CCAAT/Enhancer Binding Protein-Epsilon Reduces Atherosclerotic Lesions in LDLR-/- Mice Public Deposited ... The CCAAT/enhancer binding proteins (C/EBPs) are transcription factors involved in hematopoietic cell development and induction ... 2014) Deficiency of CCAAT/Enhancer Binding Protein-Epsilon Reduces Atherosclerotic Lesions in LDLR-/- Mice. PLoS ONE 9(1): ... Peritoneal macrophages of llee male mice have reduced mRNA expression of FABP4, a fatty acid binding protein implicated in ...
CCAAT/enhancer binding protein (C/EBP), zeta, opposite strand. Synonyms: 1110001A16Rik. Gene nomenclature, locus information, ...
CCAAT/enhancer binding protein \(\gamma\) (C/EBPγ) is a member of the C/EBP family of transcription factors, which lacks known ... CCAAT/Enhancer-Binding Protein \(\gamma\) Is a Critical Regulator of IL-1\(\beta\)-Induced IL-6 Production in Alveolar ... CCAAT/enhancer-binding protein \(\gamma\) is a critical regulator of IL-1\(\beta\)-induced IL-6 production in alveolar ... CCAAT/Enhancer-Binding Protein \(\gamma\) Is a Critical Regulator of IL-1\(\beta\)-Induced IL-6 Production in Alveolar ...
Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ... Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ... Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ... Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ...
  • PERK, double-stranded-RNA-regulated protein kinase-like ER kinase. (biochemj.org)
  • Protein limitation in vivo or amino acid deprivation of cells in culture causes a signal transduction cascade consisting of activation of the kinase GCN2 (general control nonderepressible 2), phosphorylation of eukaryotic initiation factor 2, and increased synthesis of activating transcription factor (ATF) 4 by a translational control mechanism. (semanticscholar.org)
  • A mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK)-dependent transcriptional program controls activation of the early growth response 1 (EGR1) gene during amino acid limitation. (semanticscholar.org)
  • 2015. Protein Kinase C is involved in the induction of ATP-Binding cassette transporter A1 expression by liver X receptor/retinoid X receptor agonist in human macrophages . (cardiff.ac.uk)
  • Here, we show that nuclear factor κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways regulate the expression of CCAAT/enhancer-binding protein homologous protein (CHOP), which mediates endoplasmic reticulum stress-induced apoptosis. (elsevier.com)
  • Transfection of human astroglial cells with a plasmid expressing C/EBPβ induced expression of multiple targets identified in the transcriptomic analysis of HAND brains, including dynamin-1-like protein (DNM1L) and interleukin-1 receptor-associated kinase 1. (nntc.org)
  • In this study, we demonstrated that PGE2 acts through EP4 receptor and protein kinase A to modulate CCAAT/enhancer-binding protein delta (CEBPD) abundance in astrocytes. (ncku.edu.tw)
  • Four DNA-recombinant proteins, corresponding to the DNA-binding domain of CCAAT/enhancer binding protein (C/EBP), were phosphorylated in vitro by protein kinase C (PKC). (elsevier.com)
  • Phosphorylation of C/EBP by PKC or M-kinase resulted in an attenuation of binding to a 32 P-labeled CCAAT oligodeoxynucleotide. (elsevier.com)
  • Studies on the mechanisms underlying these changes have unraveled the involvement of mitogen-activated protein kinase (MAPK) pathway in the disease process. (springer.com)
  • AMP-activated protein kinase (AMPK) is a key modulator to maintain the cellular as well as whole-body energy balance. (rsc.org)
  • ERICH4 has proposed phosphorylation at serine amino acids 28 and 96 and amino acid 36, a threonine, by casein kinase II and protein kinase c, respectively. (wikipedia.org)
  • C/EBP proteins interact with the CCAAT (cytosine-cytosine-adenosine-adenosine-thymidine) box motif, which is present in several gene promoters. (wikipedia.org)
  • In this study, the role of hypoxia-inducible factor-1 (HIF-1) signaling in the regulation of CCAAT (cytosine-cytosine-adenosine-adenosine-thymidine)/enhancer-binding protein-β (C/EBPβ) and the receptor activator of nuclear factor kappa-B ligand (RANKL) expressions in immortalized human periodontal ligament (PDL) cells was investigated. (nii.ac.jp)
  • CCAAT/enhancer-binding protein delta (CEBPD) belongs to the CCAAT/enhancer-binding protein family, and these proteins function as transcription factors in many biological processes, including cell differentiation, motility, growth arrest, proliferation, cell death, metabolism and immune responses. (biomedcentral.com)
  • CEBPD was induced in the nuclei of tubular epithelial cells in both acute and chronic hypoxic kidneys.In turn, CEBPD induction augmented HIF-1α expression and its transcriptional activity.Mechanistically, CEBPD directly bound to the HIF-1α promoter and enhanced its transcription. (nih.gov)
  • We report that CCAAT/enhancer-binding protein δ (CEBPD), a transcription factor and inflammatory response gene, is a novel HIF-1 regulator in kidney. (nih.gov)
  • Mechanistically, CEBPD directly bound to the HIF-1α promoter and enhanced its transcription. (nih.gov)
  • CCAAT/enhancer-binding protein δ (CEBPD) is expressed in hypoxic kidney tubular cells in vivo. (nih.gov)
  • The transcription factor CCAAT/enhancer-binding protein delta (CEBPD) is highly expressed in astrocytes and has been suggested to contribute to the progress of Alzheimer's disease (AD). (elsevier.com)
  • Cebpd can up-regulate p47 phox and p67 phox transcription via a direct binding on their promoters, which results in an increase in intracellular oxidative stress. (elsevier.com)
  • Background: The up-regulation of CCAAT/enhancer binding protein delta (CEBPD) has frequently been observed in macrophages in age-associated disorders, including rheumatoid arthritis (RA). (elsevier.com)
  • In vivo DNA binding assays and reporter assays showed that CEBPD up-regulated CCL20, CXCL1, IL23A and TNFAIP6 transcripts through direct binding to their promoter regions. (elsevier.com)
  • This domain is involved in dimerization and DNA binding, as are other transcription factors of the leucine zipper domain-containing family (c-Fos and c-jun). (wikipedia.org)
  • Podust LM, Krezel AM, Kim Y. Crystal structure of the CCAAT box/enhancer-binding protein beta activating transcription factor-4 basic leucine zipper heterodimer in the absence of DNA. (springer.com)
  • The CCAAT/enhancer (C/EBP) family of basic-leucine zipper (bZIP) transcription factors is a multifaceted highly-regulated system for gene regulation. (springer.com)
  • C/EBP proteins are all members of the b-ZIP family of transcription factors and share a highly homologous carboxy terminus that contains the basic and leucine zipper protein domains. (asm.org)
  • The different C/EBP family members homo- and heterodimerize through their leucine zipper regions and bind to their cognate DNA sequences through the corresponding basic regions. (asm.org)
  • CCAAT/enhancer binding protein(C/EBP) α is a family of transcription factors that all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. (creativebiomart.net)
  • a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. (creativebiomart.net)
  • The intronless C/EBPβ gene encodes a single mRNA that produces three protein isoforms, C/EBPβ-1, -2, and -3, which share a common basic-leucine zipper domain at their C-terminus, but are distinguished at their N-termini by the in-frame methionine codon used to initiate translation. (semanticscholar.org)
  • CCAAT/enhancer binding proteins (C/EBPs) are a family of leucine zipper, transcription factors that bind to DNA as homodimers and heterodimers. (elsevier.com)
  • CCAAT/enhancer-binding protein β (C/EBPβ), is a member of a family of transcription factors consisting of six structurally related basic leucine-zipper DNA-binding proteins. (biomedcentral.com)
  • This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. (genecards.org)
  • The family of C/EBP ( CCAAT-enhancer binding protein ) basic leucine zipper transcription factors includes C/EBPα, C/EBPβ, C/EBPδ, C/EBPγ and C/EBPε. (activemotif.jp)
  • Each member exhibits similar DNA-binding specificities and contains a leucine zipper dimerization region. (activemotif.jp)
  • Their amino end includes a transcriptional activation domains, as well as the carboxyl terminal area includes a simple leucine zipper theme that forms homo- or hetero-dimers and enables binding to DNA. (cancercurehere.com)
  • Regulation of Id2 expression by CCAAT/enhancer binding protein beta. (nih.gov)
  • Translocation of C/EBPbeta into the nucleus, which was achieved by using a system utilizing the fusion protein between C/EBPbeta and the ligand-binding domain of the human estrogen receptor (C/EBPbeta-ERT), demonstrated the rapid induction of endogenous Id2 expression. (nih.gov)
  • Induction of endogenous Id2 expression by the C/EBPβ-ERT fusion protein. (nih.gov)
  • They promote the expression of certain proteins through interaction with DNA . (bionity.com)
  • The nuclear factor for IL-6 expression (NF-IL6) was discovered as a member of the CCAAT-enhancer binding proteins (C/EBP) in 1990, from which it derived its current name: C/EBP-β (Akira et al. (springer.com)
  • 1990 ). C/EBP-β was first found to bind to an interleukin 1 (IL1) responsive element necessary for IL-6 expression (Akira et al. (springer.com)
  • Expression pattern of the CCAAT/enhancer-binding proteins C/EBP-alpha, C/EBP-beta and C/EBP-delta in the human placenta. (springer.com)
  • Chakrabarty A, Roberts MR. Ets-2 and C/EBP-beta are important mediators of ovine trophoblast Kunitz domain protein-1 gene expression in trophoblast. (springer.com)
  • Lala-Tabbert N, Fu D, Wiper-Bergeron N. Induction of CCAAT/enhancer-binding protein beta expression with the phosphodiesterase inhibitor isobutylmethylxanthine improves myoblast engraftment into dystrophic muscle. (springer.com)
  • Marchildon F, Lamarche E, Lala-Tabbert N, St-Louis C, Wiper-Bergeron N. Expression of CCAAT/enhancer binding protein beta in muscle satellite cells inhibits myogenesis in cancer cachexia. (springer.com)
  • Bronchial smooth muscle (BSM) cells of asthmatic patients have an impaired expression of CCAAT/enhancer binding protein (C/EBP) alpha, which is associated with increased proliferation. (nih.gov)
  • Although CHOP lacks the ability to bind classical C/EBP-binding DNA elements, CHOP forms a heterodimeric complex with other C/EBP family members, and it is thus an influential repressor of C/EBPβ-linked gene expression ( 5 , 6 ). (jimmunol.org)
  • By gel shift assay and Western blotting, we found that the DNA binding complex and protein expression of C/EBPβ and δ were increased by LPS treatment, but these results were not found for Sp1. (jimmunol.org)
  • The increase of C/EBPβ and δ proteins and the enhancement of transactivation activity of C/EBPβ and δ by LPS treatment, at least in part, explain the activation of IL-10 gene expression. (jimmunol.org)
  • In mouse monocytes/macrophages, the Sp1 binding site residing at −89 to −78 bp of the IL-10 promoter is also critical for its basal and LPS-induced expression ( 22 , 23 ). (jimmunol.org)
  • Expression of APP genes is known to be regulated by the CCAAT Enhancer Binding Protein (C/EBP) family of transcription factors, which consists of six members (a-Q. Several studies have implicated C/EBP5 as an important regulator of APP gene transcription during inflammation. (bl.uk)
  • However, unlike endogenous C/EBP5 mRNA and protein expression, both of which were induced by IL-1, the activity of the human C/EBP5 gene promoter was not stimulated by this cytokine, despite being responsive to IL-6 action. (bl.uk)
  • Conclusions- IL-6 increases hepatic PAI-1 expression mediated by the −232- to −210-bp region of the promoter containing a C/EBPδ binding site. (ahajournals.org)
  • Peritoneal macrophages of llee male mice have reduced mRNA expression of FABP4, a fatty acid binding protein implicated in atherosclerosis. (oregonstate.edu)
  • While C/EBP proteins are expressed in many human tissues, high levels of C/EBP mRNA and protein expression are limited to only a few cell types, including cells of the myeloid lineage. (asm.org)
  • In fact, C/EBP proteins are intimately involved in the regulation of myelocytic/monocytic gene expression. (asm.org)
  • Growth-dependent inhibition of CCAAT enhancer-binding protein (C/EBP alpha) gene expression during hepatocyte proliferation in the regenerating liver and in culture. (asm.org)
  • We have focused on CCAAT enhancer-binding protein (C/EBP alpha), a transcription factor which is highly abundant in normal liver and is considered to regulate expression of many genes, including some involved in energy metabolism (S. L. McKnight, M. D. Lane, and S. Gluecksohn-Walsh. (asm.org)
  • In another study, we found that ceramide decreased nuclear hepatic nuclear factor-1 (HNF1), whose binding to the HNF1-response element in the GSTA2 gene was responsible for the constitutive and inducible gene expression. (aspetjournals.org)
  • Abgent has over fifteen years of experience producing recombinant proteins in E. coli and mammalian cells (CHO and HEK293, etc), and we have added a powerful yeast expression platform to our menu of services. (abgent.com)
  • With state-of-the art molecular biology and protein biochemistry labs, we work with our clients to rapidly evaluate in parallel to identify the optimal expression system for candidate proteins. (abgent.com)
  • Elevated ATF4 expression, in the absence of other signals, is sufficient for transcriptional induction via CCAAT enhancer-binding protein-activating transcription factor response elements. (semanticscholar.org)
  • These expression vectors were cotransfected with a chloramphenicol acetyltransferase reporter gene and, in all cell lines tested, only the GAL-C/EBPε-(1-115) fusion protein significantly activated expression from the chloramphenicol acetyltransferase reporter gene. (elsevier.com)
  • Expression vectors containing the repression domain of C/EBPε strongly inhibited gene transcription from TK, SV40, and adenovital major late promoters bearing GALA binding sites. (elsevier.com)
  • The transcription factor, CCAAT/enhancer binding protein beta (C/EBPbeta), regulates the expression of genes involved in proliferation and terminal differentiation. (mysciencework.com)
  • Differentiation-induced gene expression in 3T3-L1 preadipocytes: CCAAT/enhancer binding protein interacts with and activates the promoters of two adipocyte-specific genes. (elsevier.com)
  • As an approach to defining the mechanism responsible for activating the expression of these genes, we investigated the binding of nuclear factors to the promoters of two differentiation-induced genes, the 422(aP2) and stearoyl-CoA desaturase 1 (SCD1) genes. (elsevier.com)
  • Transcription factors form a vital link in the chain of regeneration, converting injury-induced stress signals into downstream protein expression via gene regulation. (frontiersin.org)
  • This protein is a transcription factor, which means that it attaches (binds) to specific regions of DNA and helps control the activity (expression) of certain genes. (nih.gov)
  • Impaired DNA binding interferes with the protein's ability to regulate gene expression and impairs its tumor suppressor function. (nih.gov)
  • Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. (genecards.org)
  • To regulate gluconeogenesis, functionally cooperates with FOXO1 binding to IRE-controlled promoters and regulating the expression of target genes such as PCK1 or G6PC. (genecards.org)
  • Sleadd, Isaac Martin, "CCAAT/Enhancer-Binding Protein Delta (C/EBP-delta) Expression in Antarctic Fishes: Implications for Cell Cycle and Apoptosis" (2013). (pdx.edu)
  • We demonstrate that stimulatory signals can change the DNA methylation status at a CCAAT/enhancer binding protein (CEBP) binding site and a transcription start site and activate expression of the angiotensinogen gene (AGT). (elsevier.com)
  • A CEBP binding site in the human AGT promoter was hypomethylated in tissues with high expression of AGT, but not in those with low expression. (elsevier.com)
  • Excess circulating aldosterone upregulated AGT expression and was accompanied by DNA hypomethylation around a CEBP binding site and a transcription start site in human visceral adipose tissue. (elsevier.com)
  • High salt intake led to upregulation of Agt expression, DNA hypomethylation around 2 CEBP binding sites and a transcription start site, and decreased DNA methylation activity in rat visceral adipose tissue. (elsevier.com)
  • C/EBPs can bind DNA as homodimers and recruit coactivators to promote gene expression. (activemotif.jp)
  • 2016. The role of mitogen-activated protein kinases and sterol receptor coactivator-1 in TGF-β-regulated expression of genes implicated in macrophage cholesterol uptake . (cardiff.ac.uk)
  • Main Activation of CCAAT/Enhancer-binding Protein (C/EBP) Expression by C/EBPbeta during Adipogenesis. (booksc.org)
  • The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. (atcgbio.com)
  • Shao, C, Lawrence, MC & Cobb, MH 2010, ' Regulation of CCAAT/enhancer-binding protein homologous protein (CHOP) expression by interleukin-1β in pancreatic βcells ', Journal of Biological Chemistry , vol. 285, no. 26, pp. 19710-19719. (elsevier.com)
  • Transcription factors belonging to the CCAAT-enhancer binding protein (C/EBP) family have been implicated in the regulation of gene expression during differentiation, development and disease. (cf.ac.uk)
  • Endothelial function was examined, and the left main coronary artery atherosclerosis was quantified by stereology and semiquantitative immunohistochemistry to determine the endothelial expression of proteins related to the NO, renin-angiotensin, endoplasmic reticulum, and oxidative stress systems, as well as apoptosis. (uniss.it)
  • Electrophoretic mobility shift assays showed that C/EBPα was the major protein that bound to this site and, consistent with its expression pattern, the binding was reduced when the cells were exposed to IL‐6. (cf.ac.uk)
  • Newly developed omics approaches hold potential to identify mechanisms driving neuropathogenesis of HIV in the ART era.METHODS: In this study, using 33 postmortem frontal cortex (FC) tissues, neuropathological, molecular, and biochemical analyses were used to determine cellular localization and validate expression levels of the prolific transcription factor (TF), CCAAT enhancer binding protein (C/EBP) β, in brain tissues from HIV+ cognitively normal and HAND cases. (nntc.org)
  • Our data showed that cortisol-induced 11β-HSD1 mRNA expression dose dependently in amnion fibroblasts, which could be completely blocked both by the mRNA transcription inhibitor 5,6-dichlorobenzimidazole riboside and by the glucocorticoid receptor (GR) antagonist RU486, and partially blocked by global inhibition of CCAAT/enhancer-binding proteins (C/EBPs) with transfection of C/EBP-specific dominant-negative expression CMV500 plasmid (AC/EBP) into the cells. (uthscsa.edu)
  • In conclusion, we demonstrated that GR and C/EBPα were involved in cortisol-induced 11β-HSD1 mRNA expression via binding to 11β-HSD1 promoter in amnion fibroblasts, which may cast a feed-forward production of cortisol in the fetal membranes at the end of gestation. (uthscsa.edu)
  • The aim of this study was to examine the correlation between CCAAT- enhancer binding proteins (C/EBPs) and leptin gene expression in response to insulin deprivation in preadipocytes and adipocytes. (umn.edu)
  • Northern-blotting and Western blot analysis were used to study leptin mRNA and C/EBP protein expression in cultures, respectively. (umn.edu)
  • Insulin deprivation from Days 3-4 reduced leptin mRNA and C/EBP-α protein expression: Treatment with 850 nM or 10 nM insulin from Days 3-9 induced leptin mRNA and C/EBP-α expression at a similar level. (umn.edu)
  • Chen, XL , Dean, RG & Hausman, GJ 1999, ' Expression of leptin mRNA and CCAAT-enhancer binding proteins in response to insulin deprivation during preadipocyte differentiation in primary cultures of porcine stromal-vascular cells ', Domestic Animal Endocrinology , vol. 17, no. 4, pp. 389-401. (umn.edu)
  • Increased concentration and expression of TNF- α , interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1) are evident in adipocyte dysfunction and insulin resistance [ 11 ]. (hindawi.com)
  • In addition, IL-1β represses the expression of matrix proteins in cartilage, such as type II, type IX and type XI collagen and cartilage-derived retinoic acid-sensitive protein (CD-RAP), leading to degradation of the cartilage structures. (biomedcentral.com)
  • C/EBP-β expression vectors were also found to downregulate the reporter construct containing the promoter and enhancer of the type II collagen gene. (biomedcentral.com)
  • Expression of matrix proteins are influenced by availability of both positive and negative transacting factors. (biomedcentral.com)
  • The CCAAT enhancer binding protein beta (C/EBP-β) increased the expression of miR-16 after I/R injury. (nature.com)
  • The ChIP and luciferase promoter assay indicated that about −1.0 kb to −0.5 kb upstream of miR-16 genome promoter region containing C/EBP-β binding motif transcriptionally regulated miR-16 expression. (nature.com)
  • CCAAT/enhancer binding protein δ (C/EBPδ) regulation and expression. (yasni.com)
  • 15,16 Upon activation, AMPK might downregulate an activity of sterol regulatory element binding protein-1 (SREBP1) through protein interaction and/or phosphorylation, and subsequently inhibits target molecules for SREBP1 including fatty acid synthase (FAS) expression, leading to reduced lipogenesis and lipid accumulation. (rsc.org)
  • According to STRING analysis, ERICH4 has multiple predicted interactions with other proteins including proteins with associated immune function and expression within the gastrointestinal tract or testes from textmining. (wikipedia.org)
  • CCAAT/enhancer-binding protein homologous protein (CHOP) is a crucial stress-responsive factor in various mucosal injuries, including cellular translational stress conditions. (jimmunol.org)
  • CCAAT/enhancer-binding protein (C/EBP) homologous protein (CHOP)/growth arrest and DNA damage-inducible gene 153 are C/EBP family transcription factors that are markedly induced in response to genotoxic agents such as UV irradiation and alkylating agents ( 1 , 2 ). (jimmunol.org)
  • Arsenic trioxide sensitizes human glioma cells, but not normal astrocytes, to TRAIL-induced apoptosis via CCAAT/enhancer-binding protein homologous protein-dependent DR5 up-regulation. (virtualtrials.com)
  • Our results also indicate that an increase in CCAAT/enhancer binding protein homologous protein (CHOP) protein levels precedes As(2)O(3)-induced DR5 up-regulation. (virtualtrials.com)
  • However, the role of CCAAT-Enhancer-Binding Protein Homologous Protein (CHOP) signaling in liver IR injury still remains unclear. (atcmeetingabstracts.com)
  • Objective To explore the temporal and spatial distribution of CCAAT/enhancer-binding protein homologous protein (CHOP) and calnexin (CNX) in the dentate gyrus of mesial temporal lobe epilepsy (mTLE) mouse model. (bvsalud.org)
  • High dietary taurine reduces apoptosis and atherosclerosis in the left main coronary artery: association with reduced CCAAT/enhancer binding protein homologous protein and total plasma homocysteine but not lipidemia. (uniss.it)
  • Taurine reduces left main coronary artery wall pathology associated with decreased plasma total homocysteine, methionine, apoptosis, and normalization of CCAAT/enhancer binding protein homologous protein. (uniss.it)
  • In reporter assays, transactivation of the Id2 promoter by C/EBPbeta was observed and, among three potential C/EBPbeta binding sites found in the 2.3 kb Id2 promoter region, the most proximal element was responsible for the transactivation. (nih.gov)
  • In the Id2 promoter region spanning positions −2248 to +84 from the transcription initiation site, there are three potential C/EBPβ binding sites, named CβE1 (−445 to −436), CβE2 (−81 to −73) and CβE3 (−73 to −65), as indicated by arrows in the schematic representation of pGL2-Id2/A. The numbers shown on the left of the respective reporter constructs indicate the positions from the transcription initiation site. (nih.gov)
  • Nuclear protein binding sites in the mouse adiponectin promoter. (diabetesjournals.org)
  • NF-Y and C/EBP are bound to the adiponectin promoter in vitro. (diabetesjournals.org)
  • Previous studies have revealed that LPS can activate transcription of the IL-10 gene promoter through an SV40 promoter factor 1 (Sp1) binding site in mouse macrophage RAW264.7. (jimmunol.org)
  • Systematic deletion assay of the promoter demonstrated that the region (−239 to −210 bp) containing a putative CCAAT/enhancer-binding protein (C/EBP) binding site was necessary. (ahajournals.org)
  • 3 PAI-1 promoter contains transcription factor binding sites responsible for inducible expressions. (ahajournals.org)
  • 10 CCAAT/enhancer-binding proteins (C/EBPs) binding motifs are identified in the promoter regions of most acute-phase protein genes. (ahajournals.org)
  • Thus, HIV-1 LTR ATF/CREB binding site sequence variation may modulate cellular signaling at the viral promoter through the C/EBP pathway. (asm.org)
  • The promoter elements of many monocyte-specific genes contain C/EBP binding sites, including macrophage inflammatory protein 1 alpha, tumor necrosis factor alpha ( 32 ), IL-6 ( 6 , 27 , 38 ), and IL-8 ( 27 , 36 ). (asm.org)
  • One differentiation-induced nuclear factor interacts specifically with a single binding site in the promoter of each gene. (elsevier.com)
  • Bacterially expressed recombinant C/EBP binds to the same site at which the differentiation-specific nuclear factor interacts within the promoter of each gene. (elsevier.com)
  • Taken together, CEBP binding initiates chromatin relaxation and transcription, which are followed by DNA demethylation around a CEBP binding site and a transcription start site in the AGT promoter. (elsevier.com)
  • Although phosphorylated STAT3 was not detected to directly bind to FTO promoter, it was found to interact with C/EBP beta. (biomedcentral.com)
  • We have, therefore, investigated this aspect using the Xenopus laevis CCAAT/enhancer binding protein‐α (C/EBPα) gene promoter as a model. (cf.ac.uk)
  • We report that CCAAT/enhancer-binding protein (C/EBP)-β interacts with ARE/EpRE in the rGST-Ya promoter and that aryl hydrocarbon receptor (AhR) is present within the protein complex binding to the C/EBP site. (elsevier.com)
  • Specific nucleotide mutations of the putative glucocorticoid responsive element or CCAAT in this promoter region attenuated the induction by cortisol. (uthscsa.edu)
  • Moreover, chromatin immunoprecipitation assay and electrophoretic mobility shift assay showed that GR and C/EBPα but not C/EBPβ could bind this promoter region upon cortisol stimulation of amnion fibroblasts. (uthscsa.edu)
  • TCM caused a 5.7-fold induction of the −517-bp promoter II construct, whereas site-directed mutagenesis of a CCAAT/enhancer binding protein (C/EBP) binding site (−317/−304 bp) abolished both baseline and TCM-induced activities. (aacrjournals.org)
  • This is, at least in part, mediated by a TCM-induced up-regulation and enhanced binding of C/EBPβ to a promoter II regulatory element. (aacrjournals.org)
  • C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. (uniprot.org)
  • Human CCAAT/enhancer-binding protein ε (C/EBPε), a new member of the C/EBP family, significantly upregulates both the mim-1 and human myeloperoxidase promoters, suggesting an important role for C/EBPε in the transcriptional regulation of a subset of myeloid-specific genes. (elsevier.com)
  • DNase I footprinting and gel retardation analysis identified two binding regions within the promoters of each gene that interact with nuclear factors present in differentiated 3T3-L1 adipocytes. (elsevier.com)
  • Reciprocally E2F1 blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. (genecards.org)
  • The protein CEBP-alpha encoded by CEBPA intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. (atcgbio.com)
  • CCAAT/enhancer binding protein-β (C/EBPβ) and NF-E2-related factor-2 (Nrf2) are both involved in the regulation of the genes encoding phase II detoxification enzymes including glutathione S -transferase (GST). (aspetjournals.org)
  • Dimerization of the dominant-negative C/EBPbeta-liver-enriched inhibitory protein (LIP) isoform with the C/EBPbeta-liver-enriched activating protein (LAP) isoform inhibits the transcriptional activation of genes involved in differentiation. (mysciencework.com)
  • The encoded protein is important in the regulation of genes involved in immune and inflammatory responses, and may be involved in the regulation of genes associated with activation and/or differentiation of macrophages. (nih.gov)
  • The transcription factors CCAAT/enhancer binding proteins (C/EBP) α, β and δ have been shown to be expressed in brain and to be involved in regulation of inflammatory genes in concert with nuclear factor κB (NF-κB). (biomedcentral.com)
  • We have shown that acute phase protein (APP) genes are induced during the intestinal APR, and that the CCAAT/enhancer binding protein family of transcription factors are involved in APP regulation. (usherbrooke.ca)
  • Binds directly to the consensus DNA sequence 5-T[TG]NNGNAA[TG]-3 acting as an activator on distinct target genes (PubMed:11242107). (genecards.org)
  • A cDNA microarray study looked at the Notothenioid fish Trematomus bernacchii and revealed heat sensitivities for hundreds of genes, two of which code for members of the CCAAT/Enhancer-binding protein (C/EBP) family of transcription factors. (pdx.edu)
  • The pocket proteins bind to E2F and inhibit the activation of E2F-responsive genes through two mechanisms (reviewed in refs. (pnas.org)
  • These E2Fs are thought to play a key role in the repression of responsive genes through the recruitment of pocket proteins and their associated histone deacetylases. (pnas.org)
  • Surprisingly, ABIN1-deficient macrophages exhibit normal regulation of major proinflammatory signaling pathways and mediators but show selective deregulation of the transcription factor CCAAT/enhancer binding protein β (C/EBPβ) and its target genes, such as colony-stimulating factor 3 (Csf3), nitric oxide synthase, inducible (Nos2), and S100 calcium-binding protein A8 (S100a8). (elsevier.com)
  • Additionally, cooperation of C/EBPα with other Stat3-activated proteins are required for the induction of some G-CSF responsive genes including lysozyme M and the G-CSF receptor. (elsevier.com)
  • Cellular perturbations like metabolic insults and unfolded proteins can be registered by the endoplasmic reticulum (ER) and result in ER stress responses, which can lead eventually to apoptosis. (biochemj.org)
  • Multiple protein isoforms, including truncated, dominant negatives, are generated by translation of the C/EBPβ transcript or via proteolytic cleavage of the full-length C/EBPβ protein. (elsevier.com)
  • The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. (genecards.org)
  • The human gene produces 4 isoforms (32, 30, 27 and 14 kDa C/EBP proteins), and function of these isoforms purchase OSI-420 differs. (cancercurehere.com)
  • CCAAT-enhancer-binding proteins (or C/EBPs) is a family of transcription factors composed of six members, named from C/EBPα to C/EBPζ. (wikipedia.org)
  • Once bound to DNA, C/EBPs can recruit so-called co-activators (such as CBP) that in turn can open up chromatin structure or recruit basal transcription factors. (wikipedia.org)
  • The dimerization is necessary to enable C/EBPs to bind specifically to DNA through a palindromic sequence in the major groove of the DNA. (wikipedia.org)
  • C/EBPs proteins are involved in different cellular responses like in the control of cellular proliferation, growth and differentiation, metabolism , immunology and many others. (bionity.com)
  • The CCAAT/enhancer binding proteins (C/EBPs) are transcription factors involved in hematopoietic cell development and induction of several inflammatory mediators. (oregonstate.edu)
  • CCAAT/enhancer binding proteins (C/EBPs) are a family of factors that regulate cell growth and differentiation. (elsevier.com)
  • CCAAT/Enhancer binding proteins (C/EBPs) play important roles in the regulation of cell growth and differentiation. (nih.gov)
  • A family of transcription factors, CCAAT/enhancer binding proteins (C/EBPs), assists in oriLyt-mediated DNA replication during gammaherpesvirus reactivation. (bvsalud.org)
  • We also analyzed levels of proteins that control translation of CEBPA mRNA, namely heterogeneous nuclear ribonucleoprotein E2, calreticulin, eukaryotic translation initiation factor (eIF4E), and 4E binding protein. (nih.gov)
  • The CEBPA gene provides instructions for making a protein called CCAAT enhancer-binding protein alpha. (nih.gov)
  • This shortened protein is produced from one copy of the CEBPA gene in each cell, and it is believed to interfere with the tumor suppressor function of the normal protein produced from the second copy of the gene. (nih.gov)
  • The somatic CEBPA gene mutations that have been identified in leukemia cells generally decrease the DNA-binding ability of CCAAT enhancer-binding protein alpha. (nih.gov)
  • CEBPA (CCAAT Enhancer Binding Protein Alpha) is a Protein Coding gene. (genecards.org)
  • sh-LTviral- CEBPA (CCAAT/enhancer binding protein, alpha) (cat#SH3001-11A) is the function-validated shRNA-CEBPA (CCAAT/enhancer binding protein, alpha) lentivirus particle. (atcgbio.com)
  • Should the Mouse CCAAT/Enhancer Binding Protein Beta (CEBPb) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement. (bioemm.com)
  • Description: A sandwich quantitative ELISA assay kit for detection of Mouse CCAAT/Enhancer Binding Protein Beta (CEBPb) in samples from tissue homogenates, cell lysates or other biological fluids. (bioemm.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human CCAAT/Enhancer Binding Protein Beta (CEBPb) in Tissue homogenates, cell lysates and other biological fluids. (bioemm.com)
  • 2. Kovács KA, Steinmann M, Magistretti PJ, Halfon O, Cardinaux JR., CCAAT/enhancer-binding protein family members recruit the coactivator CREB-binding protein and trigger its phosphorylation, J Biol Chem. (thefullwiki.org)
  • Three other truncated forms of C/EBP, C/EBP87, C/EBP87(S-C), and C/EBP60, were studied to define the sites of phosphorylation affecting DNA binding. (elsevier.com)
  • Phosphorylation of the C/EBP87, containing sites Ser 299 and Ser 277 , and C/EBP60, containing only site Ser 299 , by PKC also resulted in attenuation of DNA binding. (elsevier.com)
  • In contrast, phosphorylation of C/EBP87(S-C), which retained Ser 277 but had a Cys in place of Ser 299 , had no effect on DNA binding. (elsevier.com)
  • To elucidate the structure and function of C/EBPε in transcriptional activation, amino acid residues 1-115, 147-249, or 1-249 of C/EBPε were fused to the yeast GAL4 DNA binding domain. (elsevier.com)
  • The typical CCAAT-enhancer binding protein forms dimers and consists of an activation domain, a DNA-binding basic region, and a leucine-rich dimerization domain (LEUCINE ZIPPERS). (uams.edu)
  • In addition, CREB-1 homodimers bind to the ATF/CREB site and recruit C/EBP dimers to their cognate weak binding sites. (asm.org)
  • C/EBP family consist of several related proteins, C/EBP α, β, γ, δ, that form homodimers and that form heterodimers with each other. (creativebiomart.net)
  • The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. (genecards.org)
  • These data suggest that CREB proteins are involved in the regulation of granulopoiesis via C/EBPβ upregulation. (harvard.edu)
  • Therefore, the highly conserved DNA binding domains explain how C/EBP family members can recognize similar DNA sequences in in vitro and in vivo DNA binding assays [ 7 - 10 ] but show diverse functions in cells, which may result from interaction with various proteins involving in transcriptional regulation or post-translational modifications. (biomedcentral.com)
  • Absence of the tumor suppressor function of CCAAT enhancer-binding protein alpha is believed to disrupt the regulation of blood cell production, leading to the uncontrolled production of abnormal cells that occurs in acute myeloid leukemia. (nih.gov)
  • The E2F and pocket protein families are known to play an important role in the regulation of both cellular proliferation and terminal differentiation. (pnas.org)
  • Chen, YH & Ramos, KS 2000, ' A CCAAT/enhancer-binding protein site within antioxidant/electrophile response element along with CREB-binding protein participate in the negative regulation of rat GST-Ya, gene in vascular smooth muscle cells ', Journal of Biological Chemistry , vol. 275, no. 35, pp. 27366-27376. (elsevier.com)
  • A gene on chromosome 12q13.1-q13.2 that encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors, which form heterodimers with other C/EBP members-e.g. (thefreedictionary.com)
  • It can also form heterodimers with the related protein CEBP-alpha. (nih.gov)
  • It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. (atcgbio.com)
  • Granulocyte colony-stimulating factor (G-CSF) promotes granulopoiesis and granulocytic differentiation, and Stat3 is the principle Stat protein activated by G-CSF. (elsevier.com)
  • The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. (nih.gov)
  • This protein is a member of the BZIP family, C/EBP subfamily. (biocompare.com)
  • As this element contains the CCAAT/enhancer-binding protein (C/EBP) motif, the function of C/EBP-β was examined. (biomedcentral.com)
  • The direct binding of C/EBP-β to the C/EBP motif was confirmed by electrophoretic mobility shift assay. (biomedcentral.com)
  • C/EBP proteins also contain activation domains at the N-terminus and regulatory domains. (wikipedia.org)
  • C/EBPβ can interact with CREB, NF-κB, and other proteins, leading to a trans-activation potential. (wikipedia.org)
  • Although the Sp1 site is essential for activation of the IL-10 gene, DNA binding activity of Sp1 is not changed after LPS treatment ( 22 ). (jimmunol.org)
  • The immutability of Sp1-DNA binding activity is insufficient to explain the mechanism of gene activation in mouse macrophages. (jimmunol.org)
  • CCAAT/enhancer binding protein \(\gamma\) (C/EBPγ) is a member of the C/EBP family of transcription factors, which lacks known activation domains. (harvard.edu)
  • Most importantly, sequence variation at the ATF/CREB binding site affected basal LTR activity as well as LTR function following interleukin-6 stimulation, a treatment that leads to increases in C/EBP activation. (asm.org)
  • TransAM ® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. (activemotif.jp)
  • Gene deletion of individual C/EBP family members has demonstrated an essential role for C/EBPβ in normal mammary development, while transgenic and overexpression studies provide evidence that the dominant-negative C/EBPβ-liver-enriched inhibitory protein isoform induces proliferation in mammary epithelial cells. (elsevier.com)
  • Zahnow, CA 2002, ' CCAAT/enhancer binding proteins in normal mammary development and breast cancer ', Breast Cancer Research , vol. 4, no. 3, pp. 113-121. (elsevier.com)
  • The CCAAT/enhancer binding protein epsilon protein is a reported synonym for the human gene CEBPE, encoding CCAAT enhancer binding protein epsilon. (biocompare.com)
  • C/EBP proteins are involved in different cellular responses, such as in the control of cellular proliferation, growth and differentiation, in metabolism, and in immunity. (wikipedia.org)
  • The transcription factor CCAAT/Enhancer-Binding Protein alpha (C/EBPa) coordinates proliferation arrest and differentiation of myeloid progenitors and adipocytes. (hu-berlin.de)
  • The CCAAT-enhancer binding protein (C/EBP) has now been found to promote the terminal differentiation of adipocytes. (sciencemag.org)
  • The transcription factor CCAAT/enhancer binding protein (C/EBP)β is critical for normal growth and differentiation of the mammary gland. (semanticscholar.org)
  • Several lines of evidence indicate that the differentiation-induced nuclear factor is CCAAT/enhancer binding protein (C/EBP), a DNA-binding protein first isolated from rat liver. (elsevier.com)
  • CCAAT enhancer-binding protein alpha is involved in the maturation (differentiation) of certain blood cells. (nih.gov)
  • This finding definitively separates the known, positive role of pRB in adipogenesis from its cell cycle function and shows that this pocket protein is required to act downstream of E2F4 in the differentiation process. (pnas.org)
  • It is currently unclear whether the developmental defects reflect a requirement for E2F4 and/or E2F5 in cell cycle control or whether these proteins play a more direct role in the differentiation process. (pnas.org)
  • In contrast, PPARγ-regulating CHOP was a positive modulator of HuR protein export from nuclei. (jimmunol.org)
  • Taken together, the results indicate that ribotoxin-induced CHOP protein is positively associated with production of proinflammatory cytokine IL-8, but it downregulates PPARγ action, subsequently allowing the cytosolic translocation of HuR protein and stabilization of IL-8 mRNA in gut epithelial cells. (jimmunol.org)
  • Recently, CHOP protein was reported to be a crucial mediator involved in pathogenesis of human inflammatory bowel disease (IBD)-like ulcerative colitis by mediating production of proinflammatory cytokines ( 10 ). (jimmunol.org)
  • Incubation of wild-type CHO-K1 or CT-expressing MT58 cells at 40°C did not induce differences in CHOP protein levels in time. (biochemj.org)
  • These results suggest that PC depletion in MT58 induces the ER-stress-related protein CHOP, without raising a general ER stress response. (biochemj.org)
  • Both CHOP mRNA and protein increase in β cells treated with IL-1β. (elsevier.com)
  • Electrophoretic mobility shift assay (EMSA) identified this element as a core sequence to which C/EBPbeta binds. (nih.gov)
  • The sequence comparison of these sites with the consensus binding site (), TT/GNNGNAAT/G, is shown in the inset. (nih.gov)
  • View conserved domains detected in this protein sequence using CD-search. (nih.gov)
  • See the reference protein sequence for CCAAT/enhancer-binding protein delta (NP_005186.2). (nih.gov)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • Additionally, sequence variation at C/EBP site I, which lies immediately upstream of the distal nuclear factor kappa B site and immediately downstream of a binding site for activating transcription factor (ATF)/cyclic AMP response element binding protein (CREB), has been shown to affect HIV-1 long terminal repeat (LTR) activity. (asm.org)
  • A class of proteins that were originally identified by their ability to bind the DNA sequence CCAAT. (uams.edu)
  • Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and sequence-specific DNA binding . (genecards.org)
  • The 5′-flanking sequence of the chicken FTO gene contains nine predicted binding sites for CCAAT/enhancer binding protein beta (C/EBP beta) and one for signal transducer and activator of transcription 3 (STAT3). (biomedcentral.com)
  • The alpha helix segments span from amino acids 2-9, 21-24, 47-58, 61-94, and 104-111 in the protein sequence. (wikipedia.org)
  • Program analysis in SWISS-Model proposes a tertiary structure for ERICH4 by matching the protein against the template of NLRP6 with a sequence identity of 25.79%, sequence similarity of 0.30, and coverage of 0.43 for amino acids 43-92 in ERICH4. (wikipedia.org)
  • First, low amounts of CREB-1 and C/EBP appear to heterodimerize and bind to a site consisting of a half site from both the ATF/CREB and C/EBP binding sites. (asm.org)
  • This interaction is reciprocal, since C/EBP dimer binding to a strong C/EBP site leads to enhanced CREB-1 recruitment to ATF/CREB sites that are weakly bound by CREB. (asm.org)
  • In addition, the transcripts of CRE binding (CREB) family proteins were readily detected in hematopoietic stem/progenitor cells. (harvard.edu)
  • CREB was upregulated, phosphorylated and bound to the CREs in response to GM-CSF stimulation. (harvard.edu)
  • Overexpression of CREB-binding protein (CBP) nullified repression of rGST-Ya transcription. (elsevier.com)
  • van der Krieken SE, Popeijus HE, Mensink RP, Plat J. CCAAT/enhancer binding protein beta in relation to ER stress, inflammation, and metabolic disturbances. (springer.com)
  • The initial phase of inflammation, the acute phase response (APR), is characterised by changes in levels of serum acute phase response proteins (APPs). (bl.uk)
  • Inflammation and hypoxia linked to renal injury by CCAAT/enhancer-binding protein δ. (nih.gov)
  • Results Af -exposed mice showed that ER stress markers, unfolded protein response (UPR)-related proteins, phosphorylated Akt, generation of mitochondrial reactive oxygen species (mtROS), eosinophilic allergic inflammation, and airway hyperresponsiveness (AHR) were increased in the lung. (bmj.com)
  • A PI3K-δ inhibitor reduced Af -induced increases in ER stress markers, UPR-related proteins, allergic inflammation and AHR in mice. (bmj.com)
  • CCAAT/enhancer-binding protein zeta is a protein that in humans is encoded by the CEBPZ gene . (wikidoc.org)
  • 13 Statins can modify fibrinolytic potential of endothelial cells via inhibition of geranylgeranylated Rho protein 14 and act on PAI-1 transcription 12 and inhibit inflammatory transcription factors. (ahajournals.org)
  • CCL20, IL23A, CXCL1 and TNFAIP6 contributed to the migration and proliferation of synoviocytes, and the latter two proteins were involved in tube formation of endothelial cells. (elsevier.com)
  • Taurine failed to improve hyperlipidemia, endothelial function, or endothelial proteins related to the NO, renin-angiotensin, and oxidative stress systems. (uniss.it)
  • HMDB and 5-AzadC Combination Reverses Tumor Suppressor CCAAT/Enhancer-Binding Protein Delta to Strengthen the Death of Liver Cancer Cells. (nih.gov)
  • Mounting evidence suggests that alterations in the ratio of the C/EBPβ-liver-enriched inhibitory protein isoform and the C/EBPβ-liver-enriched activating protein isoform may play a role in the development of breast cancer. (elsevier.com)
  • A role for CCAAT/enhancer binding protein beta-liver-enriched. (mysciencework.com)
  • A role for CCAAT/enhancer binding protein beta-liver-enriched inhibitory protein in mammary epithelial cell proliferation. (mysciencework.com)
  • The "full-length" 38 kd C/EBPbeta LAP ("Liver-enriched Activator Protein") isoform is the predominant C/EBPbeta protein isoform in mammary tumor whole cell lysates, however, the truncated 20 kd C/EBPbeta LIP ("Liver-enriched Inhibitory Protein") isoform is also present at detectable levels (mean LAP:LIP ratio 5.3:1). (nih.gov)
  • Increasing amounts of rat liver nuclear extract are assayed using the TransAM C/EBP α/β Kit in the presence or absence of competitor oligonucleotides containing the wild-type or a mutated form of the C/EBP consensus binding site. (activemotif.jp)
  • C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. (creativebiomart.net)
  • Electrophoretic mobility shift assay and chromatin immunoprecipitation assay demonstrated that C/EBPα, C/EBPβ, and C/EBPδ were involved in protein-DNA complex formation in intact cells. (ahajournals.org)
  • We found that C/EBP α and β bind to the CCAAT boxes in the MHV-68 oriLyt core region both in vitro and in vivo, as demonstrated by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. (bvsalud.org)
  • The C/EBP family of proteins includes at least eight different proteins, many of which are important activators of transcription. (asm.org)
  • Second, it is a specific property of E2F4, and not other E2Fs, and it occurs independently of E2F4's ability to interact with pocket proteins. (pnas.org)
  • It does not interact with the pocket proteins and has been shown to play a role in vertebrate patterning through interaction with the mammalian polycomb complex ( 3 , 4 ). (pnas.org)
  • Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. (atcgbio.com)
  • Previous studies using loss-of-function mutants revealed that CCAAT/enhancer-binding protein α (C/EBPα) and PU.1 are potential regulators for hematopoietic stem cells (HSCs). (elsevier.com)
  • Your search returned 52 CCAAT enhancer binding protein delta ELISA ELISA Kit across 12 suppliers. (biocompare.com)
  • E2F acts as a co-repressor of another transcription factor, C/EBPa, without binding to cis-regulatory elements, but by direct protein-protein interactions that abolish the binding of C/EBPa to DNA. (hu-berlin.de)
  • No experimentally confirmed protein interactions yet. (wikipedia.org)
  • These results demonstrate that the "full-length" C/EBPbeta LAP isoform is the predominant C/EBPbeta protein isoform expressed in mouse mammary gland in vivo and mouse mammary epithelial cell cultures in vitro. (nih.gov)
  • This mechanism of transcriptional repression occurs independent of pocket proteins. (hu-berlin.de)
  • These results suggest that C/EBP-β play an important role in the distinct effects of IL-1β : the promotion of inflammatory reaction and the repression of cartilage-specific proteins in joint disease. (biomedcentral.com)
  • Second, the resultant E2F⋅pocket protein complexes can actively repress transcription through recruitment of histone deacetylase and methylase activities. (pnas.org)
  • The full-length isoform of the C/EBPβ protein (LAP) activates the MafB gene, whereas the short isoform (LIP) suppresses it. (wikipedia.org)
  • ERICH4 has one different protein-encoding transcript variant, or isoform. (wikipedia.org)
  • Transcriptional activators include C/EBP α ( 4 ), C/EBP β (nuclear factor interleukin-6), (IL-6) ( 2 , 9 , 12 , 40 ), C/EBP δ ( 42 ), C/EBP ɛ ( 44 ), and C/EBP-related protein 1 (CRP-1) ( 42 ). (asm.org)
  • Based on our recent report that PPARγ is a negative regulator of mRNA stability of IL-8, PPARγ was linked to a notable mRNA stabilizing protein, HuR, since ribotoxin-induced IL-8 mRNA is stabilized by HuR protein. (jimmunol.org)
  • When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. (activemotif.jp)
  • A primary antibody specific for an epitope on the bound and active form of the transcription factor is then added followed by subsequent incubation with secondary antibody and Developing Solution to provide an easily quantified, sensitive colorimetric readout (Figure 1). (activemotif.jp)
  • This work demonstrates an association of C/EBPa to the RNA Pol I transcription factor UBF1, both proteins retained in large chromosomal foci. (hu-berlin.de)
  • Hypoxia had no effects on the secretion of interleukin (IL)-1β, IL-6, IL-8, IL-17A, tumor necrosis factor-alpha, macrophage migration inhibitory factor, monocyte chemoattractant protein-1, and macrophage colony-stimulating factor in the culture media. (nii.ac.jp)
  • In contrast to the definitive role of the transcription factor, CCAAT/Enhancer binding protein α (C/EBPα), in steady-state granulopoiesis, previous findings have suggested that granulopoiesis during emergency situations, such as infection, is dependent on C/EBPβ. (harvard.edu)
  • CCAAT-BINDING FACTOR is structurally distinct type of CCAAT-enhancer binding protein consisting of a trimer of three different subunits. (uams.edu)
  • N-terminal nonsense mutations result in a dominant negative C/EBP-alpha protein while C-terminal mutations reduce the DNA-binding potential of this transcription factor. (genecards.org)
  • Deletion of the transcription factor CCAAT/enhancer binding protein (C/EBP)β results in a severe inhibition of lobuloalveolar development in the mouse mammary gland. (elsevier.com)
  • The TransAM format is perfect for assaying transcription factor binding to a consensus-binding site. (activemotif.jp)
  • Activated nuclear extract is added to each well and the transcription factor of interest binds specifically to this bound oligonucleotide. (activemotif.jp)
  • Activated transcription factor in the cell extract binds to the consensus-binding site on the oligo immobilized in the well. (activemotif.jp)
  • CCAAT/enhancer binding protein δ is a transcription factor that has recently been hypothesized to play a detrimental role in outcome of meningitis caused by S. pneumoniae . (springer.com)
  • Finally, the enhancer factor, Sox9, known to be downregulated by IL-1, was shown to bind adjacent to the C/EBP site competing with C/EBP binding. (biomedcentral.com)
  • Anti-CCAAT/enhancer binding protein epsilon antibodies can be readily obtained from commercial sources. (biocompare.com)
  • Your search returned 241 CCAAT/enhancer binding protein epsilon Antibodies across 25 suppliers. (biocompare.com)
  • Check out links to articles that cite our custom service antibodies, peptides, and proteins in major peer-reviewed journals, organized by research category. (abgent.com)
  • The TransAM C/EBP α/β Kit contains antibodies specific for the active form of C/EBPα and C/EBPβ when bound to the target DNA. (activemotif.jp)
  • Mice deficient for Id2, a negative regulator of basic helix-loop-helix (bHLH) transcription factors, exhibit a defect in lactation due to impaired lobuloalveolar development during pregnancy, similar to the mice lacking the CCAAT enhancer binding protein (C/EBP) beta. (nih.gov)
  • Recent observations have shown two CCAAT/enhancer binding protein (C/EBP) binding sites to be critically important for efficient human immunodeficiency virus type 1 (HIV-1) replication within cells of the monocyte/macrophage lineage, a cell type likely involved in transport of the virus to the brain. (asm.org)
  • Additional studies indicated that these two C/EBP binding sites were required for replication of an infectious HIV-1 molecular clone in the U-937 cell line as well as in primary cells of the monocyte/macrophage lineage. (asm.org)
  • 1 Plasminogen activator inhibitor-1 (PAI-1), the physiological inhibitor of fibrinolysis, is an acute-phase protein that impairs fibrinolysis. (ahajournals.org)
  • The 32 and 30 kDa C/EBP works as transcriptional activator, the 27 kDa protein as transcriptional repressor, and the 14 kDa form as dominant-negative regulator [27]. (cancercurehere.com)