A CCAAT-enhancer-binding protein found in LIVER; INTESTINES; LUNG and ADIPOSE TISSUE. It is an important mediator of INTERLEUKIN-6 signaling.
A class of proteins that were originally identified by their ability to bind the DNA sequence CCAAT. The typical CCAAT-enhancer binding protein forms dimers and consists of an activation domain, a DNA-binding basic region, and a leucine-rich dimerization domain (LEUCINE ZIPPERS). CCAAT-BINDING FACTOR is structurally distinct type of CCAAT-enhancer binding protein consisting of a trimer of three different subunits.
A CCAAT-enhancer-binding protein found in LIVER; ADIPOSE TISSUE; INTESTINES; LUNG; ADRENAL GLANDS; PLACENTA; OVARY and peripheral blood mononuclear cells (LEUKOCYTES, MONONUCLEAR). Experiments with knock-out mice have demonstrated that CCAAT-enhancer binding protein-alpha is essential for the functioning and differentiation of HEPATOCYTES and ADIPOCYTES.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
A member of the C-EBP protein family of transcription factors. It plays a key role in G0 PHASE mammary EPITHELIAL CELL growth arrest, and it is involved in transcriptional regulation of INTERLEUKIN 1; INTERLEUKIN 6; and TUMOR NECROSIS FACTOR-ALPHA.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A CCAAT-enhancer binding protein that is induced by DNA DAMAGE and growth arrest. It serves as a dominant negative inhibitor of other CCAAT-enhancer binding proteins.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Cells in the body that store FATS, usually in the form of TRIGLYCERIDES. WHITE ADIPOCYTES are the predominant type and found mostly in the abdominal cavity and subcutaneous tissue. BROWN ADIPOCYTES are thermogenic cells that can be found in newborns of some species and hibernating mammals.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The differentiation of pre-adipocytes into mature ADIPOCYTES.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Established cell cultures that have the potential to propagate indefinitely.
An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.
A continuous cell line that is a substrain of SWISS 3T3 CELLS developed though clonal isolation. The mouse fibroblast cells undergo an adipose-like conversion as they move to a confluent and contact-inhibited state.
Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. These elements may be found in both promoter and enhancer regions.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
A nuclear transcription factor. Heterodimerization with RETINOID X RECEPTOR ALPHA is important in regulation of GLUCOSE metabolism and CELL GROWTH PROCESSES. It is a target of THIAZOLIDINEDIONES for control of DIABETES MELLITUS.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Formation of MYELOID CELLS from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW via MYELOID STEM CELLS. Myelopoiesis generally refers to the production of leukocytes in blood, such as MONOCYTES and GRANULOCYTES. This process also produces precursor cells for MACROPHAGE and DENDRITIC CELLS found in the lymphoid tissue.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
Heterotrimeric GTP-binding protein subunits that tightly associate with GTP-BINDING PROTEIN GAMMA SUBUNITS. A dimer of beta and gamma subunits is formed when the GTP-BINDING PROTEIN ALPHA SUBUNIT dissociates from the GTP-binding protein heterotrimeric complex. The beta-gamma dimer can play an important role in signal transduction by interacting with a variety of second messengers.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.
DNA-binding motifs formed from two alpha-helixes which intertwine for about eight turns into a coiled coil and then bifurcate to form Y shaped structures. Leucines occurring in heptad repeats end up on the same sides of the helixes and are adjacent to each other in the stem of the Y (the "zipper" region). The DNA-binding residues are located in the bifurcated region of the Y.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.
Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.
Nucleic acid sequences involved in regulating the expression of genes.
A forkhead transcription factor that regulates expression of metabolic GENES and is involved in EMBRYONIC DEVELOPMENT. Mutations in HNF-3beta have been associated with CONGENITAL HYPERINSULINISM.
An organochlorine compound that was formerly used as an insecticide. Its manufacture and use has been discontinued in the United States. (From Merck Index, 11th ed)
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. This protein functions to integrate both calcium and cAMP signals.
An early local inflammatory reaction to insult or injury that consists of fever, an increase in inflammatory humoral factors, and an increased synthesis by hepatocytes of a number of proteins or glycoproteins usually found in the plasma.
A component of NF-kappa B transcription factor. It is proteolytically processed from NF-kappa B p105 precursor protein and is capable of forming dimeric complexes with itself or with TRANSCRIPTION FACTOR RELA. It regulates expression of GENES involved in immune and inflammatory responses.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A cell line derived from cultured tumor cells.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A DNA-directed RNA polymerase found in BACTERIA. It is a holoenzyme that consists of multiple subunits including sigma factor 54.
Transport proteins that carry specific substances in the blood or across cell membranes.
Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
An anti-inflammatory 9-fluoro-glucocorticoid.
A human cell line established from a diffuse histiocytic lymphoma (HISTIOCYTIC LYMPHOMA, DIFFUSE) and displaying many monocytic characteristics. It serves as an in vitro model for MONOCYTE and MACROPHAGE differentiation.
A family of DNA binding proteins that regulate expression of a variety of GENES during CELL DIFFERENTIATION and APOPTOSIS. Family members contain a highly conserved carboxy-terminal basic HELIX-TURN-HELIX MOTIF involved in dimerization and sequence-specific DNA binding.

C/EBPalpha regulates generation of C/EBPbeta isoforms through activation of specific proteolytic cleavage. (1/788)

C/EBPalpha and C/EBPbeta are intronless genes that can produce several N-terminally truncated isoforms through the process of alternative translation initiation at downstream AUG codons. C/EBPbeta has been reported to produce four isoforms: full-length 38-kDa C/EBPbeta, 35-kDa LAP (liver-enriched transcriptional activator protein), 21-kDa LIP (liver-enriched transcriptional inhibitory protein), and a 14-kDa isoform. In this report, we investigated the mechanisms by which C/EBPbeta isoforms are generated in the liver and in cultured cells. Using an in vitro translation system, we found that LIP can be generated by two mechanisms: alternative translation and a novel mechanism-specific proteolytic cleavage of full-length C/EBPbeta. Studies of mice in which the C/EBPalpha gene had been deleted (C/EBPalpha-/-) showed that the regulation of C/EBPbeta proteolysis is dependent on C/EBPalpha. The induction of C/EBPalpha in cultured cells leads to induced cleavage of C/EBPbeta to generate the LIP isoform. We characterized the cleavage activity in mouse liver extracts and found that the proteolytic cleavage activity is specific to prenatal and newborn livers, is sensitive to chymostatin, and is completely abolished in C/EBPalpha-/- animals. The lack of cleavage activity in the livers of C/EBPalpha-/- mice correlates with the decreased levels of LIP in the livers of these animals. Analysis of LIP production during liver regeneration showed that, in this system, the transient induction of LIP is dependent on the third AUG codon and most likely involves translational control. We propose that there are two mechanisms by which C/EBPbeta isoforms might be generated in the liver and in cultured cells: one that is determined by translation and a second that involves C/EBPalpha-dependent, specific proteolytic cleavage of full-length C/EBPbeta. The latter mechanism implicates C/EBPalpha in the regulation of posttranslational generation of the dominant negative C/EBPbeta isoform, LIP.  (+info)

CCAAT/enhancer-binding proteins are mediators in the protein kinase A-dependent activation of the decidual prolactin promoter. (2/788)

In the course of decidualization, human endometrial stromal cells (ESC) activate the alternative upstream promoter of the decidual prolactin (dPRL) gene. The dPRL promoter is induced by the protein kinase A pathway in a delayed fashion via the region -332/-270 which contains two overlapping consensus binding sequences, B and D, for CCAAT/enhancer-binding proteins (C/EBP). Here we show that sites B and D both bind C/EBPbeta and -delta from ESC nuclear extracts. When decidualization of cultured ESC was induced by treatment with 8-Br-cAMP, complex formation on sites B and D was enhanced. Western blot analysis revealed an elevation of both C/EBPbeta isoforms, liver-enriched activator protein and liver-enriched inhibitory protein, with a delayed onset between 8 and 24 h of cAMP treatment, while C/EBPdelta expression remained unaffected. Cyclic AMP-mediated activation of dPRL promoter construct dPRL-332/luc3 was abrogated by mutation of sites B and D at -310/-285. An expression vector for liver-enriched activator protein potently induced transcription of dPRL-332/luc3 and further enhanced cAMP-mediated induction, while liver-enriched inhibitory protein expression vector abolished the cAMP response, implying that C/EBPs serve as mediators in the delayed cAMP signal transduction to the dPRL promoter. The ratio between activating and repressing isoforms is likely to dictate the transcriptional output.  (+info)

CUG repeat binding protein (CUGBP1) interacts with the 5' region of C/EBPbeta mRNA and regulates translation of C/EBPbeta isoforms. (3/788)

The transcription factor CCAAT/enhancer binding protein beta, C/EBPbeta, plays a significant role in the regulation of hepatocyte growth and differentiation. A single mRNA coding for C/EBPbeta produces several protein isoforms. Two pathways for generation of low molecular weight C/EBPbeta isoforms have been described: specific proteolytic cleavage and initiation of translation from different AUG codons of C/EBPbeta mRNA. A truncated C/EBPbeta isoform, LIP, is induced in rat livers in response to partial hepatectomy (PH) via the alternative translation mechanism. Here we present evidence that CUG repeat binding protein, CUGBP1, interacts with the 5' region of C/EBPbeta mRNA and regulates translation of C/EBPbeta isoforms. Two binding sites for CUGBP1 are located side by side between the first and second AUG codons of C/EBPbeta mRNA. One binding site is observed in an out of frame short open reading frame (sORF) that has been previously shown to regulate initiation of translation from different AUG codons of C/EBPbeta mRNA. Analysis of cytoplasmic and polysomal proteins from rat liver after PH showed that CUGBP1 is associated with polysomes that translate low molecular weight isoforms of C/EBPbeta. The binding activity of CUGBP1 to the 5' region of C/EBPbeta mRNA shows increased association with these polysomal fractions after PH. Addition of CUGBP1 into a cell-free translation system leads to increased translation of low molecular weight isoforms of C/EBPbeta. Our data demonstrate that CUGBP1 protein is an important component for the regulation of initiation from different AUG codons of C/EBPbeta mRNA.  (+info)

The C/EBP bZIP domain can mediate lipopolysaccharide induction of the proinflammatory cytokines interleukin-6 and monocyte chemoattractant protein-1. (4/788)

C/EBPalpha, beta, and delta are all expressed by bone marrow-derived macrophages. Ectopic expression of any of these transcription factors is sufficient to confer lipopolysaccharide (LPS)-inducible expression of interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) to a B lymphoblast cell line, which normally lacks C/EBP factors and does not display LPS induction of proinflammatory cytokines. Thus, the activities of C/EBPalpha, beta, and delta are redundant in regard to expression of IL-6 and MCP-1. Surprisingly, the bZIP region of C/EBPbeta, which lacks any previously described activation domains, can also confer LPS-inducible expression of IL-6 and MCP-1 in stable transfectants. Transient transfections reveal that the bZIP regions of C/EBPbeta, C/EBPdelta, and, to a lesser extent, C/EBPalpha can activate the IL-6 promoter and augment its induction by LPS. Furthermore, the transdominant inhibitor, LIP, can activate expression from the IL-6 promoter. The ability of the C/EBPbeta bZIP region to activate the IL-6 promoter in transient transfections is completely dependent upon an intact NF-kappaB-binding site, supporting a model where the bZIP protein primarily functions to augment the activity of NF-kappaB. Replacement of the leucine zipper of C/EBPbeta with that of GCN4 yields a chimeric protein that can dimerize and specifically bind to a C/EBP consensus sequence, but shows a markedly reduced ability to activate IL-6 and MCP-1 expression. These results implicate the leucine zipper domain in some function other than dimerization with known C/EBP family members, and suggest that C/EBP redundancy in regulating cytokine expression may result from their highly related bZIP regions.  (+info)

Inflammatory versus proliferative processes in epidermis. Tumor necrosis factor alpha induces K6b keratin synthesis through a transcriptional complex containing NFkappa B and C/EBPbeta. (5/788)

Epidermal keratinocytes respond to injury by becoming activated, i.e. hyperproliferative, migratory, and proinflammatory. These processes are regulated by growth factors and cytokines. One of the markers of activated keratinocytes is keratin K6. We used a novel organ culture system to show that tumor necrosis factor alpha (TNFalpha) induces the expression of K6 protein and mRNA in human skin. Multiple isoforms of K6 are encoded by distinct genes and have distinct patterns of expression. By having shown previously that proliferative signals, such as epidermal growth factor (EGF), induce expression of the cytoskeletal protein keratin K6b, we here demonstrate that the same isoform, K6b, is also induced by TNFalpha, a proinflammatory cytokine. Specifically, TNFalpha induces the transcription of the K6b gene promoter. By using co-transfection, specific inhibitors, and antisense oligonucleotides, we have identified NFkappaB and C/EBPbeta as the transcription factors that convey the TNFalpha signal. Both transcription factors are necessary for the induction of K6b by TNFalpha and act as a complex, although only C/EBPbeta binds the K6b promoter DNA. By using transfection, site-directed mutagenesis, and footprinting, we have mapped the site that responds to TNFalpha, NFkappaB, and C/EBPbeta. This site is separate from the one responsive to EGF and AP1. Our results show that the proinflammatory (TNFalpha) and the proliferative (EGF) signals in epidermis separately and independently regulate the expression of the same K6b keratin isoform. Thus, the cytoskeletal responses in epidermal cells can be precisely tuned by separate proliferative and inflammatory signals to fit the nature of the injuries that caused them.  (+info)

NF-IL6 and CRE elements principally account for both basal and interleukin-1 beta-induced transcriptional activity of the proximal 528bp of the PGHS-2 promoter in amnion-derived AV3 cells: evidence for involvement of C/EBP beta. (6/788)

Prostaglandin H synthase (PGHS)-2 promoter fragments (-528 to +9 bp and 5' unidirectional deletions thereof) were cloned upstream of the chloramphenicol acetyl-transferase (CAT) reporter gene. These were transfected into amnion-derived AV3 cells. The region, -528 to -203, which includes NF-kappa B sites, had little influence on CAT expression. The region, -203 and -52, however, was responsible for most of the basal promoter activity and also conferred responsiveness to interleukin (IL)-1 beta (>3-times basal). Point mutations of NF-IL6 and cAMP response element (CRE) in this region reduced both basal and IL-1 beta-stimulated production of CAT; dual mutation eliminated IL-1 beta responsiveness. Factors in nuclear extracts from control or IL-1 beta-stimulated AV3 cells specifically complexed the NF-IL6 and CRE sequences. However, the NF-IL6 and CRE oligonucleotides cross-competed, suggesting a common factor. C/EBP beta was identified by supershift assay as interacting with both sequences. To a lesser extent C/EBP alpha and delta also interacted with the NF-IL6 site. However, CRE binding protein (CREB), was absent from the complex with the CRE. In conclusion, NF-IL6 and CRE elements principally account in AV3 amnion cells for basal and IL-1 beta-inducible transcriptional activity of the proximal 528 bp of the PGHS-2 promoter, while NF-kappa B elements play no substantial role. C/EBPs, particularly C/EBPbeta, are implicated in control of PGHS-2 transcription through the NF-IL6 and CRE sites.  (+info)

Multiple regulatory elements in the murine stromelysin-3 promoter. Evidence for direct control by CCAAT/enhancer-binding protein beta and thyroid and retinoid receptors. (7/788)

Stromelysin-3 (ST3) belongs to the matrix metalloproteinase (MMPs) family, a protease family involved in tissue remodeling. Although this family of enzymes is regulated by nuclear receptors, few hormone-responsive elements have been demonstrated in MMP promoters. In order to identify regulatory elements and/or factors that control the expression of the mouse st3 gene, we have analyzed genomic sequences encompassing 5 kilobase pairs of the ST3 promoter. Analysis of these sequences revealed several CCAAT/enhancer-binding proteins (C/EBP) and retinoic acid-responsive elements (RAREs), as well as one thyroid-responsive element. However, in contrast to most MMP promoters, no AP-1-binding sites were identified. Specific binding activities were demonstrated for all elements. Consistent with previous reports, retinoid X receptor is required for maximal binding to the ST3 RAREs and the TRE. The ST3-C/EBP element was shown to mediate dose-dependent promoter activation by C/EBPbeta. Among the RAREs, the proximal DR1-RARE was shown to be sufficient for ST3 promoter activation by ligand-bound retinoid receptors, whereas the two distal DR2-RAREs appear to be involved more in the control of base-line promoter activity. Accordingly, ST3 expression was induced by retinoic acid and was reduced in cells where specific retinoic acid receptors had been inactivated. The involvement of these conserved regulatory elements is discussed in the context of physiological or pathological situations associated with st3 expression. Our findings therefore assign to C/EBP, retinoids, and thyroid hormone important roles in the regulation of ST3 gene expression.  (+info)

ERK1 and ERK2 activate CCAAAT/enhancer-binding protein-beta-dependent gene transcription in response to interferon-gamma. (8/788)

Interferons (IFNs) regulate the expression of a number of cellular genes by activating the JAK-STAT pathway. We have recently discovered that CCAAAT/enhancer-binding protein-beta (C/EBP-beta) induces gene transcription through a novel IFN response element called the gamma-IFN-activated transcriptional element (Roy, S. K., Wachira, S. J., Weihua, X., Hu, J., and Kalvakolanu, D. V. (2000) J. Biol. Chem. 275, 12626-12632. Here, we describe a new IFN-gamma-stimulated pathway that operates C/EBP-beta-regulated gene expression independent of JAK1. We show that ERKs are activated by IFN-gamma to stimulate C/EBP-beta-dependent expression. Sustained ERK activation directly correlated with C/EBP-beta-dependent gene expression in response to IFN-gamma. Mutant MKK1, its inhibitors, and mutant ERK suppressed IFN-gamma-stimulated gene induction through the gamma-IFN-activated transcriptional element. Ras and Raf activation was not required for this process. Furthermore, Raf-1 phosphorylation negatively correlated with its activity. Interestingly, C/EBP-beta-induced gene expression required STAT1, but not JAK1. A C/EBP-beta mutant lacking the ERK phosphorylation site failed to promote IFN-stimulated gene expression. Thus, our data link C/EBP-beta to IFN-gamma signaling through ERKs.  (+info)

Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human CCAAT/Enhancer Binding Protein Beta (CEBPb) in samples from Tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species ...
Conserved upstream open reading frames (uORFs) are found within many eukaryotic transcripts and are known to regulate protein translation. Evidence from genetic and bioinformatic studies implicates disturbed uORF-mediated translational control in the etiology of human diseases. A genetic mouse model has recently provided proof-of-principle support for the physiological relevance of uORF-mediated translational control in mammals. The targeted disruption of the uORF initiation codon within the transcription factor CCAAT/enhancer binding protein beta (C/EBPbeta) gene resulted in deregulated C/EBPbeta protein isoform expression, associated with defective liver regeneration and impaired osteoclast differentiation. The high prevalence of uORFs in the human transcriptome suggests that intensified search for mutations within 5 RNA leader regions may reveal a multitude of alterations affecting uORFs, causing pathogenic deregulation of protein expression.. ...
THOC5 is a nuclear/cytoplasmic protein member of the spliceosome complex which potentiates C/EBP expression in adipocyte differentiation. As C/EBP family members are important regulators of myelopoiesis and THOC5 is highly expressed in neutrophil/macrophage progenitor cells we assessed the role of THOC5 in cytokine-stimulated monocytic development. M-CSF stimulated maturation of the NFS60 cell line was associated with enhanced THOC5 expression and phosphorylation. THOC5 was also shown to form a complex with C/EBPbeta. Ectopic expression of THOC5 mimicked M-CSF mediated cell maturation and enhanced protein expression of the myeloid transcription factors C/EBPbeta, C/EBPalpha, Pu-1 and also GAB2 (a PI-3 Kinase and macrophage development regulator). Increased THOC5 expression also mimicked M-CSF stimulated increases in the lipid second messenger PtdInsP(3). Inhibition of THOC5-induced increases in PtdInsP(3) levels abrogated the elevated levels of C/EBPbeta. Thus THOC5 expression can potentiate ...
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Stress responses are critical for estrogen (E2) to induce apoptosis in E2-deprived breast cancer cells. Nuclear factor-kappa B (NF-κB) is well known as a therapeutic target to prevent stress responses in chronic inflammatory diseases including cancer. However, whether E2 activates NF-κB to participate in stress-associated apoptosis in E2-deprived breast cancer cells is unclear. We demonstrated that E2 differentially modulates NF-κB activity in E2-deprived breast cancer cells according to the treatment time. Because E2 initially has significant potential to down modulate the NF-κB activation, it completely suppresses the tumor necrosis factor alpha (TNFα)-induced NF-κB activation. We found that E2 preferentially and constantly enhances the expression of transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) which is responsible for suppression of NF-κB activation by E2 in MCF-7:5C cells. The mTOR signaling pathway promotes repression of NF-κB by C/EBPβ which is confirmed by ...
A recent paper in Nature Medicine showed that Down syndrome brains have reduced expression of Sorting nexin 27 (SNX27) and CCAAT/enhancer binding protein beta (C/EBP beta) and identified C/EBP beta as a transcription factor for SNX27. Down syndrome results in overexpression of miR-155, a chromosome 21-encoded microRNA that negatively regulates C/EBP beta, thereby reducing SNX27 expression. SNX27 is a brain-enriched […]. ...
CCAAT/enhancer-binding protein beta is a protein that in humans is encoded by the CEBPB gene. The protein encoded by this intronless gene is a bZIP transcription factor that can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. CEBP-beta is critical for normal macrophage functioning, an important immune cell sub-type; mice unable to express CEBP-beta have macrophages that cannot differentiate (specialize) and thus are unable to perform all their biological functions - including macrophage-mediated muscle repair. ...
Mouse polyclonal antibody raised against a full-length human CEBPB protein. CEBPB (AAH21931.1, 1 a.a. ~ 345 a.a) full-length human protein. (H00001051-B01P) - Products - Abnova
CCAAT/enhancer-binding protein δ (CEBPD) is expressed in hypoxic kidney tubular cells in vivo. (a) Mice were exposed to 8% O2 for 6 h using a hypoxia chamber
Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear.
Human CEBPB partial ORF ( NP_005185, 256 a.a. - 344 a.a.) recombinant protein with GST-tag at N-terminal. (H00001051-Q01) - Products - Abnova
The gene encoding an important myeloid transcription factor is mutated in cells of acute myeloid leukemia (somatic mutation) and in patients with autosomal dominant familial acute myeloid leukemia (germline mutation).. ...
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Fasting elicits transcriptional programs in hepatocytes leading to glucose and ketone production. This transcriptional program is regulated by many transcription factors (TFs). To understands how this complex network regulates the metabolic response to fasting we aimed at isolating the enhancers and TFs dictating it. Measuring chromatin accessibility revealed that fasting massively reorganizes liver chromatin, exposing numerous fasting-induced enhancers. By utilizing computational methods in combination with dissecting enhancers features and TF cistromes, we implicated four key TFs regulating the fasting response: glucocorticoid receptor (GR), cAMP responsive element binding protein 1 (CREB1), peroxisome proliferator activated receptor alpha (PPARA) and CCAAT/enhancer binding protein beta (CEBPB). These TFs regulate fuel production by two distinctly-operating modules, each controlling a separate metabolic pathway. The gluconeogenic module operates through assisted loading whereby GR doubles the ...
TY - JOUR. T1 - Identification of transcriptional activation and repression domains in human CCAAT/enhancer-binding protein ε. AU - Williamson, Elizabeth A.. AU - Xu, Haixin N.. AU - Gombart, Adrian F.. AU - Verbeek, Walter. AU - Chumakov, Alexey M.. AU - Friedman, Alan D.. AU - Koeffler, H. Phillip. PY - 1998/6/12. Y1 - 1998/6/12. N2 - Human CCAAT/enhancer-binding protein ε (C/EBPε), a new member of the C/EBP family, significantly upregulates both the mim-1 and human myeloperoxidase promoters, suggesting an important role for C/EBPε in the transcriptional regulation of a subset of myeloid-specific genes. To elucidate the structure and function of C/EBPε in transcriptional activation, amino acid residues 1-115, 147-249, or 1-249 of C/EBPε were fused to the yeast GAL4 DNA binding domain. These expression vectors were cotransfected with a chloramphenicol acetyltransferase reporter gene and, in all cell lines tested, only the GAL-C/EBPε-(1-115) fusion protein significantly activated ...
TNF-a was originally identified as a macrophage product implicated in the metabolic disturbances of chronic inflammation and malignancy. Later on, its biological actions were shown to further extend to anorexia, weight loss, and insulin resistance (7). Elevated adipose tissue expression of TNF-a mRNA has been reported in different rodent models of obesity as well as in clinical studies involving obese patients (23). TNF-a mRNA expression is positively correlated with body adiposity as well as with hyperinsulinemia, showing positive associations with fasting insulin and triglyceride concentrations. TNF-a inhibits the expression of the transcription factor CCAAT/ enhancer binding protein-a (CEBPa) and the nuclear receptor peroxisome proliferator-activated receptor (PPAR)y2 (8,12,14). Furthermore, TNF-a stimulates the nuclear factor- kB transcription factor (NFkB), which orchestrates a series of inflammatory events, including expression of adhesion molecules on the surface of both endothelial cells ...
Macrophages play an essential role in the resolution of tissue damage through removal of necrotic cells, thus paving the way for tissue regeneration. Macrophages also directly support the formation of new tissue to replace the injury, through their acquisition of an anti-inflammatory, or M2, phenotype, characterized by a gene expression program that includes IL-10, the IL-13 receptor, and arginase 1. We report that deletion of two CREB-binding sites from the Cebpb promoter abrogates Cebpb induction upon macrophage activation. This blocks the downstream induction of M2-specific Msr1, Il10, II13ra, and Arg-1 genes, whereas the inflammatory (M1) genes Il1, Il6, Tnfa, and Il12 are not affected. Mice carrying the mutated Cebpb promoter (betaDeltaCre) remove necrotic tissue from injured muscle, but exhibit severe defects in muscle fiber regeneration. Conditional deletion of the Cebpb gene in muscle cells does not affect regeneration, showing that the C/EBPbeta cascade leading to muscle repair is muscle
Expression of CEBPB (C/EBP-beta, CRP2, IL6DBP, LAP, NFIL6, TCF5) in nasopharynx tissue. Antibody staining with CAB004213 in immunohistochemistry.
Expression of CEBPB (C/EBP-beta, CRP2, IL6DBP, LAP, NFIL6, TCF5) in cervix, uterine tissue. Antibody staining with CAB004213 in immunohistochemistry.
The CCAAT/enhancer-binding protein β (C/EBPβ) is a transcription factor, which was first identified as a regulator of differentiation and inflammatory processes mainly in adipose tissue and liver; however, its function in the brain was largely unknown for many years. Previous studies from our laboratory indicated that C/EBPβ is implicated in inflammatory process and brain injury, since mice lacking this gene were less susceptible to kainic acid-induced injury. We first performed cDNA microarrays analysis using hippocampal RNA isolated from C/EBPβ +/+ and C/EBPβ −/− mice. Immunocytochemical and immunohistochemical studies were done to evaluate C/EBPβ and C3 levels. Transient transfection experiments were made to analyze transcriptional regulation of C3 by C/EBPβ. To knockdown C/EBPβ and C3 expression, mouse astrocytes were infected with lentiviral particles expressing an shRNA specific for C/EBPβ or an siRNA specific for C3. Among the genes displaying
This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. The use of alternative in-frame non-AUG (CUG) and AUG start codons results in several protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the CUG and the first AUG start codons. [provided by RefSeq, Sep 2014 ...
The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
Having analysed data with TRANSFAC system, we may assume that the disturbed attachment of such factors as (C/EBP(CCAAT enhancer binding protein) Hoxa-3,Sp1 (serine protease inhibitor) or GATA-1, (GATA nucleotide sequence) may have an impact on IGF-1 protein synthesis, but we did not observed any significant correlation between promoter P1 polymorphism and serum IGF-1 levels ...
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TY - JOUR. T1 - Differentiation-dependent changes in levels of C/EBPβ repressors and activators regulate human papillomavirus type 31 late gene expression. AU - Gunasekharan, Vignesh. AU - Haché, Guylaine. AU - Laimins, Laimonis. N1 - Copyright: Copyright 2013 Elsevier B.V., All rights reserved.. PY - 2012/5. Y1 - 2012/5. N2 - The liver-enriched transcriptional activator protein (LAP) isoform of CCAAT/enhancer binding proteinβ (C/EBPβ) is shown to be a major activator of differentiation-dependent human papillomavirus (HPV) late gene expression, while the liver-enriched inhibitory protein (LIP) isoform negatively regulates late expression. In undifferentiated cells, LIPs act as dominant-negative repressors of late expression, and upon differentiation, LIP levels are significantly reduced, allowing LAP-mediated activation of the late promoter. Importantly, knockdown of C/EBPβ isoforms blocks activation of late gene expression from complete viral genomes upon differentiation.. AB - The ...
TY - JOUR. T1 - Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy. AU - Sun, Yuansheng. AU - Day, Richard. AU - Periasamy, Ammasi. PY - 2011/9. Y1 - 2011/9. N2 - Fluorescence lifetime imaging microscopy (FLIM) is now routinely used for dynamic measurements of signaling events inside living cells, including detection of protein-protein interactions. An understanding of the basic physics of fluorescence lifetime measurements is required to use this technique. In this protocol, we describe both the time-correlated single photon counting and the frequency-domain methods for FLIM data acquisition and analysis. We describe calibration of both FLIM systems, and demonstrate how they are used to measure the quenched donor fluorescence lifetime that results from FÃ ¶rster resonance energy transfer (FRET). We then show how the FLIM-FRET methods are used to detect the dimerization of the transcription factor CCAAT/enhancer binding protein-Î ± in ...
ABSTRACT: Fluorescence lifetime imaging microscopy (FLIM) is now routinely used for dynamic measurements of signaling events inside living cells, including detection of protein-protein interactions. An understanding of the basic physics of fluorescence lifetime measurements is required to use this technique. In this protocol, we describe both the time-correlated single photon counting and the frequency-domain methods for FLIM data acquisition and analysis. We describe calibration of both FLIM systems, and demonstrate how they are used to measure the quenched donor fluorescence lifetime that results from F?rster resonance energy transfer. We then show how the FLIM-FRET methods are used to detect the dimerization of the transcription factor CCAAT enhancer binding protein-a in live mouse pituitary cell nuclei. Notably, the factors required for accurate determination and reproducibility of lifetime measurements are described. With either method, the entire protocol including specimen preparation, ...
TY - JOUR. T1 - Differentiation-induced gene expression in 3T3-L1 preadipocytes. T2 - CCAAT/enhancer binding protein interacts with and activates the promoters of two adipocyte-specific genes.. AU - Christy, R. J.. AU - Yang, V. W.. AU - Ntambi, J. M.. AU - Geiman, D. E.. AU - Landschulz, W. H.. AU - Friedman, A. D.. AU - Nakabeppu, Y.. AU - Kelly, T. J.. AU - Lane, M. D.. PY - 1989/9. Y1 - 1989/9. N2 - Previous studies have shown that differentiation of 3T3-L1 preadipocytes leads to the transcriptional activation of a group of adipose-specific genes. As an approach to defining the mechanism responsible for activating the expression of these genes, we investigated the binding of nuclear factors to the promoters of two differentiation-induced genes, the 422(aP2) and stearoyl-CoA desaturase 1 (SCD1) genes. DNase I footprinting and gel retardation analysis identified two binding regions within the promoters of each gene that interact with nuclear factors present in differentiated 3T3-L1 adipocytes. ...
Monocytes are circulating, short-lived mononuclear phagocytes, which in mice and man comprise two main subpopulations. Murine Ly6C(+) monocytes display developmental plasticity and are recruited to complement tissue-resident macrophages and dendritic cells on demand. Murine vascular Ly6C(-) monocytes patrol the endothelium, act as scavengers, and support vessel wall repair. Here we characterized population and single cell transcriptomes, as well as enhancer and promoter landscapes of the murine monocyte compartment. Single cell RNA-seq and transplantation experiments confirmed homeostatic default differentiation of Ly6C(+) into Ly6C(-) monocytes. The main two subsets were homogeneous, but linked by a more heterogeneous differentiation intermediate. We show that monocyte differentiation occurred through de novo enhancer establishment and activation of pre-established (poised) enhancers. Generation of Ly6C(-) monocytes involved induction of the transcription factor C/EBP{beta} and ...
In this work, we have shown that the transcription factor C/EBPβ directly regulates the expression of the C3 gene, and that this control could be relevant for the pro-inflammatory effects of this transcription factor. By microarray analysis and RT-PCR we showed that the hippocampal content of C3 transcripts was depleted in C/EBPβ −/− mice. The analysis of the C3 promoter showed that this gene was directly induced by C/EBPβ through a C/EBPβ consensus site located at −616/-599 position from the transcription start site. In accordance with these data, LPS induced the expression of C3 in glial cells, at least in part, through the induction of C/EBPβ since the repression of LPS-induction of C/EBPβ by shRNA interference blocked C3 increase. On the contrary, C/EBPβ overexpression by transient transfection induced C3 expression. Additionally, treatment of these cultures with LPS induced the levels of the pro-inflammatory factors IL-1β and COX-2, which were significantly reduced in those ...
CEBPA [ENSP00000427514]. CCAAT/enhancer binding protein (C/EBP), alpha; Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5-T[TG]NNGNAA[TG]-3 acting as an activator on distinct target genes. During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP). Critical for the proper development of the liver and the lung (By similarity). Necessary for terminal adipocyte differentiation, is required for postnatal maintenance of systemic energy homeostasis and lipid storage (By similarity). To regulate these different processes at the proper moment and tissue, interplays with other transcription factors and modulators. Downregulates the expression of genes that maintain cells in an undifferentiated and ...
In this study we have shown that the rat UDP-glucuronosyltransferase 2B1 gene is specifically activated by C/EBPα and that this activation is correlated with the binding of C/EBPα to an element residing between −91 and −99 bp upstream of the UGT2B1 gene transcription start site. Furthermore, we extend these findings to show that C/EBPα is essential for the expression of transcripts homologous to UGT2B1 in adult mouse liver.. This is the first example of the regulation of a UGT2B gene by a member of the C/EBP transcription factor family, and adds to our previous finding that the UGT2B1 gene promoter also interacts with and is activated by HNF1α (Hansen et al., 1997). Both C/EBP and HNF1 also interact with the early promoter of the albumin gene (Fig. 1) (Lichtsteiner et al., 1987). When these two factors are simultaneously overexpressed, there is a strong synergistic effect on transcription of this gene (Wu et al., 1994). It was shown that a specific C/EBPα activation domain was required ...
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The CCAAT/Enhancer Binding Proteins (C/EBPs) are a family of leucine-zipper transcription factors that regulate physiological processes such as energy metabolism, inflammation, cell cycle, and the development and differentiation ...
Pharmacodynamic studies, including micro-ribonucleic acid (miRNA)-181 family and target gene expression, CCAAT/enhancer binding protein (C/EBP), alpha gene (CEBPA) expression, and genes involved in erythroid ...
Another study applying ChIP-seq for two different TFs (CCAAT/enhancer-binding protein α and hepatocyte nuclear factor 4 α) in the livers of five vertebrates species provides an alternative view of conservation of TF-binding events (Schmidt et al., 2010). This work demonstrated that the majority of binding events are species specific, rather than consistently localized in conserved regions. Binding to conserved sequences in one species was rarely indicative of binding to the homologous sequence in others. These differences in binding were consistently observed between human and mouse in the livers of both species, and also in the livers of aneuploid mice harbouring human chromosome 21. Binding to the human chromosome in mouse was representative of binding to the endogenous chromosome in human, rather than binding to mouse chromosomes (Wilson et al., 2008) (Fig. 4). The differences in binding between species are therefore unlikely to be due to non-equivalence in the assayed tissue. Similarly, ...
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Neutrophil-specific granule deficiency (SGD) is a rare disorder characterized by recurrent pyogenic infections, defective neutrophil chemotaxis and bactericidal activity, and lack of neutrophil secondary granule proteins. It has been linked to a defect in the transcription factor CCAAT/enhancer binding protein (CEBP) epsilon. Recently, loss-of-function mutations in SMARCD2 were identified from SGD patients. SMARCD2 is chromatin-remodeling factor, that interacts with CEBP epsilon ...
CCAAT/enhancer binding protein zeta (mouse, aa620-633) Antibody (internal region), Peptide-affinity purified goat antibody validated in WB, E (AF3888a), Abgent
TY - JOUR. T1 - A20-binding inhibitor of NF-κB (ABIN1) controls Toll-like receptor-mediated CCAAT/enhancer-binding protein β activation and protects from inflammatory disease. AU - Zhou, Jingran. AU - Wu, Ruiqiong. AU - High, Anthony A.. AU - Slaughter, Clive A.. AU - Finkelstein, David. AU - Rehg, Jerold E.. AU - Redecke, Vanessa. AU - Häcker, Hans. PY - 2011/11/1. Y1 - 2011/11/1. N2 - Toll-like receptors (TLRs) are expressed on innate immune cells and trigger inflammation upon detection of pathogens and host tissue injury. TLR-mediated proinflammatory-signaling pathways are counteracted by partially characterized anti-inflammatory mechanisms that prevent exaggerated inflammation and host tissue damage as manifested in inflammatory diseases. We biochemically identified a component of TLR-signaling pathways, A20-binding inhibitor of NF-κB (ABIN1), which recently has been linked by genome-wide association studies to the inflammatory diseases systemic lupus erythematosus and psoriasis. We ...
CCAAT/enhancer binding protein (C/EBP), epsilon, also known as CEBPE and CRP1, is a type of ccaat-enhancer-binding protein. CEBPE is its human gene and is pro-apoptotic. The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-δ. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with specific granule deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined. GRCh38: Ensembl release 89: ENSG00000092067 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000052435 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Entrez Gene: CEBPE CCAAT/enhancer binding protein (C/EBP), epsilon. Antonson P, Stellan B, Yamanaka R, ...
We recently discovered that induction from the anti-inflammatory gene by cyclic AMP occurs through book cyclic AMP-dependent proteins kinase-independent systems involving activation of CCAAT/enhancer-binding proteins (C/EBP) transcription elements, notably C/EBP, from the cyclic AMP GEF EPAC1 as well as the ...
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TY - JOUR. T1 - Regenerating livers of old rats contain high levels of C/EBPα that correlate with altered expression of cell cycle associated proteins. AU - Timchenko, Nikolai A.. AU - Wilde, Margaret. AU - Kosai, Ken Lchiro. AU - Heydari, Ahmed. AU - Bilyeu, Timothy A.. AU - Finegold, Milton J.. AU - Mohamedali, Khalid. AU - Richardson, Arlan. AU - Darlington, Gretchen J.. N1 - Funding Information: We thank W.Harper, S.Elledge and E.Harlow for cp36 antibodies and Dr J.Albrecht for His-C-p21. We thank K.Faraj for excellent assistance in the preparation of the manuscript. This work was supported by NIH grants DK45285 (G.J.D.), AG13663 (G.J.D.), AG00765-01 (N.A.T.) and GM55188-01 (N.A.T.), by AFAR grant A 97161, by The Moran Foundation and by the Estate of Evelyn Lucille Hansen.. PY - 1998/7/1. Y1 - 1998/7/1. N2 - The nuclear transcription factor, CCAAT/enhancer binding protein α (C/EBPα) is expressed at high levels in the liver and inhibits growth in cultured cells. We have tested the ...
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Background The up-regulation of CCAAT/enhancer binding protein delta (CEBPD) has frequently been observed in macrophages in age-associated disorders, including rheumatoid arthritis (RA). However, the role of macrophage CEBPD in the pathogenesis of RA is unclear. Methodology and Principal Findings We found that the collagen-induced arthritis (CIA) score and the number of affected paws in Cebpd−/− mice were significantly decreased compared with the wild-type (WT) mice. The histological analysis revealed an attenuated CIA in Cebpd−/− mice, as shown by reduced pannus formation and greater integrity of joint architecture in affected paws of Cebpd−/− mice compared with WT mice. In addition, immunohistochemistry analysis revealed decreased pannus proliferation and angiogenesis in Cebpd−/− mice compared with WT mice. CEBPD activated in macrophages played a functional role in promoting the tube formation of endothelial cells and the migration and proliferation of synoviocytes. In vivo DNA
TY - JOUR. T1 - Enhancer-binding proteins with a forkhead-associated domain and the sigma(54) regulon in Myxococcus xanthus fruiting body development. AU - Jelsbak, Lars. AU - Givskov, Michael Christian. AU - Kaiser, D.. PY - 2005. Y1 - 2005. N2 - In response to starvation, Myxococcus xanthus initiates a developmental program that results in the formation of spore-filled, multicellular fruiting bodies. Many developmentally regulated genes in M. xanthus are transcribed from sigma(54) promoters, and these genes require enhancer-binding proteins. Here we report the finding of an unusual group of 12 genes encoding sigma(54)-dependent enhancer-binding proteins containing a forkhead-associated (FHA) domain as their N-terminal sensory domain. FHA domains in other proteins recognize phosphothreonine residues. An insertion mutation in one of these genes, Mx4885, caused a cell autonomous aggregation and sporulation defect. In-frame deletion mutants showed that the FHA domain is necessary for proper Mx4885 ...
Here, we investigated the mechanisms by which PPARδ agonists control expression of 14-3-3ε, a key antiinflammatory protein in endothelial cells.12 Our data not only provide evidence that PPARδ modulates expression of YWHAE gene and 14-3-3ε protein under resting conditions but also demonstrate that this nuclear receptor upregulates 14-3-3ε expression by targeting transcription via a PPRE-independent pathway involving colocalization of C/EBPβ and PPARδ on YWHAE promoter. Several lines of evidence support these conclusions. First, PPARδ agonists regulated YWHAE promoter activity in a concentration- and time-dependent manner. Concordantly, YWHAE promoter was upregulated by PPARδ overexpression, whereas specific PPARγ and PPARα ligands had no effect on YWHAE promoter under our experimental conditions. Second, PPARδ activation increased 14-3-3ε mRNA and protein expression in both primary and spontaneously transformed endothelial cell lines, whereas PPARδ knockdown depressed basal and ...
FIG. 4. Effect of site-specific mutations on C/EBP and NF-Y binding activity. Nuclear extracts were prepared from 293T cells overexpressing C/EBP-α or -β. For mock, the cells were transfected by pcDNA. All probes were generated by PCR with 32P-labeled antisense primer and unlabeled sense primers using site-directed mutated plasmids as templates, and purified from the polyacrylamide gel, to equalize the specific activities between probes. A: DNA oligonucleotide sequence of wild and mutated probes used in EMSA. Mutated regions are indicated by lowercase with * with their name. B: EMSA using nuclear extracts of 293T cells overexpression C/EBP-α and C/EBP-β. Wild-type (wt) and mutant (m1, m2, m3, m4, and m5) probes were incubated with 4 μg of indicated nuclear extracts. The bands corresponding to NF-Y, C/EBP-α, and C/EBP-β are marked by arrows. C: Western blot (WB) analysis of C/EBP-α and -β for validating the expression of C/EBP-α and -β. 293T cells were transfected with expression ...
Lexpressió cortical androgen dependent del KAP està afectada en hipotiroïdisme postnatal. La síntesi puntual de T3 a partir del dia 11 postnatal, comença una resposta cortical feble de KAP que va augmentant cap als dies 15-16, que és quan es produeix un pic fisiològic de T4 i el desenvolupament puberal dels ratolins. Donat que les CCAAT/Enhancer-Binding Proteins (C/EBPs) participen en respostes mitjançades per T3 i que en el promotor del KAP existeixen quatre elements de resposta consens per a C/EBPs, hem analitzat la seva participació en la resposta androgènica de KAP mitjançada per T3. La detecció de p42C/EBPa y p35C/EBPb es troba correlacionada amb lexpressió del KAP, apareixent en extractes renal nuclears de ratolins masles control i hipotiroïdals induïts amb T3 durant els dies 7-21 postnatals, però no en els hipotiroïdals no tractats. Mitjançant transfeccions transitòries es mostrava com C/EBPa i C/EBPb eren capaces dinduir respostes màximes del promotor del KAP i que ...
Choi B.H., Park G.T., Rho H.M. (1999). Interaction of hepatitis B viral X protein and CCAAT/enhancer-binding protein alpha synergistically activates the hepatitis B viral enhancer II/pregenomic promoter.. J. Biol. Chem. 274: 2858 - 2865. PubMed DOI:10.1074/jbc.274.5.2858 ...
Plasmid p-eGFP-beta 2 CP from Dr. John Coopers lab contains the insert capping protein beta 2 subunit and is published in J Cell Biol. 1998 Dec 28. 143(7):1919-30. This plasmid is available through Addgene.
CCAAT/enhancer-binding protein beta is a protein that in humans is encoded by the CEBPB gene. The protein encoded by this ... "Entrez Gene: CEBPB CCAAT/enhancer binding protein (C/EBP), beta". Ruffell D, Mourkioti F, Gambardella A, Kirstetter P, Lopez RG ... Chen GK, Sale S, Tan T, Ermoian RP, Sikic BI (April 2004). "CCAAT/enhancer-binding protein beta (nuclear factor for interleukin ... Hanlon M, Sealy L (May 1999). "Ras regulates the association of serum response factor and CCAAT/enhancer-binding protein beta ...
"G9a-mediated lysine methylation alters the function of CCAAT/enhancer-binding protein-beta". The Journal of Biological ... Nevertheless, increasing evidence suggests methylation of non-histone proteins may influence protein stability, protein-protein ... which is involved in protein-protein interactions. The ankyrin repeat domain also contains H3K9me1 and H3K9me2 binding sites. ... Euchromatic histone-lysine N-methyltransferase 1, also known as G9a-like protein (GLP), is a protein that in humans is encoded ...
Marchildon, François (2012). "CCAAT/Enhancer Binding Protein Beta is Expressed in Satellite Cells and Controls Myogenesis". ... Moreover, both quiescent and activated human satellite cells can be identified by the membrane-bound neural cell adhesion ... There is some research indicating that satellite cells are negatively regulated by a protein called myostatin. Increased levels ... Activated satellite cells also begin expressing muscle-specific filament proteins such as desmin as they differentiate. The ...
Hanlon M, Sealy L (May 1999). "Ras regulates the association of serum response factor and CCAAT/enhancer-binding protein beta ... This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the ... Serum response factor has been shown to interact with: ASCC3, ATF6, CEBPB, CREB-binding protein, ELK4, GATA4, GTF2F1, GTF2I, ... "A multifunctional DNA-binding protein that promotes the formation of serum response factor/homeodomain complexes: identity to ...
"Physical and functional association between GADD153 and CCAAT/enhancer-binding protein beta during cellular stress". The ... It is a member of the CCAAT/enhancer-binding protein (C/EBP) family of DNA-binding transcription factors. The protein functions ... Qiao D, Im E, Qi W, Martinez JD (June 2002). "Activator protein-1 and CCAAT/enhancer-binding protein mediated GADD153 ... "Physical and functional association between GADD153 and CCAAT/enhancer-binding protein beta during cellular stress". The ...
Boruk M, Savory JG, Haché RJ (Nov 1998). "AF-2-dependent potentiation of CCAAT enhancer binding protein beta-mediated ... "Entrez Gene: DBI diazepam binding inhibitor (GABA receptor modulator, acyl-Coenzyme A binding protein)". Kos M, Reid G, Denger ... Chan SW, Hong W (Jul 2001). "Retinoblastoma-binding protein 2 (Rbp2) potentiates nuclear hormone receptor-mediated ... CREB Binding Protein Interaction Interface and Its Importance for the Function of SRC1". Mol. Cell. Biol. 21 (1): 39-50. doi: ...
"Conserved amino acids within CCAAT enhancer-binding proteins (C/EBP(alpha) and beta) regulate phosphoenolpyruvate carboxykinase ... A mitochondrial isozyme of the encoded protein also has been characterized. Click on genes, proteins and metabolites below to ... Wilson HL, McFie PJ, Roesler WJ (2003). "Different transcription factor binding arrays modulate the cAMP responsivity of the ... 2002). "Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site". J. Mol. Biol. ...
Eaton EM, Sealy L (August 2003). "Modification of CCAAT/enhancer-binding protein-beta by the small ubiquitin-like modifier ( ... "A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy ... a novel Axin-binding protein, is involved in downregulation of beta-catenin". Molecular and Cellular Biology. 22 (11): 3803-19 ... Dobreva G, Dambacher J, Grosschedl R (December 2003). "SUMO modification of a novel MAR-binding protein, SATB2, modulates ...
Eaton EM, Sealy L (Aug 2003). "Modification of CCAAT/enhancer-binding protein-beta by the small ubiquitin-like modifier (SUMO) ... Kim J, Cantwell CA, Johnson PF, Pfarr CM, Williams SC (Oct 2002). "Transcriptional activity of CCAAT/enhancer-binding proteins ... It is a ubiquitin-like protein and functions in a manner similar to ubiquitin in that it is bound to target proteins as part of ... which is an axin-binding protein promoting beta-catenin degradation". The Journal of Biological Chemistry. 276 (42): 39060-6. ...
"The CCAAT enhancer-binding protein (C/EBP)beta and Nrf1 interact to regulate dentin sialophosphoprotein (DSPP) gene expression ... NFE2L1 binds DNA as heterodimers with one of small Maf proteins (MAFF, MAFG, MAFK). NFE2L1 has been shown to interact with C- ... β-TrCP also binds to the DSGLC motif, a highly conserved region of CNC-bZIP proteins, in order to poly-ubiquitinate NFE2L1 ... Zhang Y, Lucocq JM, Hayes JD (Mar 2009). "The Nrf1 CNC/bZIP protein is a nuclear envelope-bound transcription factor that is ...
Holland MP, Bliss SP, Berghorn KA, Roberson MS (2004). "A role for CCAAT/enhancer-binding protein beta in the basal regulation ... Homeobox protein DLX-3 is a protein that in humans is encoded by the DLX3 gene. Dlx3 is a crucial regulator of hair follicle ... Park GT, Denning MF, Morasso MI (2001). "Phosphorylation of murine homeodomain protein Dlx3 by protein kinase C". FEBS Lett. ... "AP-2 gamma and the homeodomain protein distal-less 3 are required for placental-specific expression of the murine 3 beta- ...
"Transforming growth factor-beta inhibits adipocyte differentiation by Smad3 interacting with CCAAT/enhancer-binding protein (C/ ... CCAAT/enhancer-binding protein delta is a protein that in humans is encoded by the CEBPD gene. The protein encoded by this ... "CCAAT/enhancer-binding protein family members recruit the coactivator CREB-binding protein and trigger its phosphorylation". ... "Entrez Gene: CEBPD CCAAT/enhancer binding protein (C/EBP), delta". Gery S, Tanosaki S, Hofmann WK, Koppel A, Koeffler HP (Feb ...
Podust LM, Krezel AM, Kim Y (January 2001). "Crystal structure of the CCAAT box/enhancer-binding protein beta activating ... "Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal ... "Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal ... The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein ...
... a new inhibitory partner of CCAAT/enhancer-binding protein beta, implicated in adipocyte differentiation". The Journal of ... Coiled-coil domain-containing protein 85B is a protein that in humans is encoded by the CCDC85B gene. Hepatitis delta virus ( ... Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi: ... "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): ...
Chen, GK; Sale, S; Tan, S; Ermoian, RP; Sikic, BI (2004). "CCAAT/enhancer-binding protein beta (nuclear factor for interleukin ... He discovered that deletion of aa335 changes the drug-binding spectrum and is integral to the pharmacophore of P-gp. He also ... 6) transactivates the human MDR1 gene by interaction with an inverted CCAAT box in human cancer cells". Molecular Pharmacology ...
"Src homology 2 domain-containing inositol-5-phosphatase and CCAAT enhancer-binding protein beta are targeted by miR-155 in B ... "Src homology 2 domain-containing inositol-5-phosphatase and CCAAT enhancer-binding protein beta are targeted by miR-155 in B ... the protein-encoding mRNA for the transcriptional regulator Pu.1-protein, elevation of Pu.1 protein predisposes defective IgG1 ... Following Dicer cleavage, an Argonaute (Ago) protein binds to the short RNA duplexes, forming the core of a multi-subunit ...
"Functional cooperation of simian virus 40 promoter factor 1 and CCAAT/enhancer-binding protein beta and delta in ... Sp1 and cAMP-response-element-binding protein-binding protein (CBP/p300)". The Biochemical Journal. 339 ( Pt 3) (3): 751-8. doi ... gene is mediated by interactions of Msx1 protein with a multi-protein transcriptional complex containing TATA-binding protein, ... The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The ...
CCAAT/enhancer-binding protein alpha is a protein encoded by the CEBPA gene in humans. CCAAT/enhancer-binding protein alpha is ... It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma, as well as distinct transcription factors ... For details on the CCAAT structural motif in gene enhancers and on CCAAT/Enhancer Binding Proteins see the specific page. The ... One category of mutations prevent CCAAT/enhancer-binding protein alpha DNA binding by altering its COOH-terminal basic leucine ...
Zuo Y, Qiang L, Farmer SR (March 2006). "Activation of CCAAT/enhancer-binding protein (C/EBP) alpha expression by C/EBP beta ... binding protein], PACT (protein activator of the interferon-induced protein kinase), the SMN complex, fragile X mental ... significantly inhibited adipogenesis and repressed induction of the master regulators PPARγ and CCAAT/enhancer-binding protein ... These proteins have three highly positively charged regions, termed AT hooks, that bind the minor groove of AT-rich DNA ...
CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. Ccaat-enhancer-binding proteins ... The C/EBP family consist of several related proteins, C/EBP alpha, C/EBP beta, C/EBP gamma, and C/EBP delta, that form ... CCAAT/enhancer-binding protein gamma is a protein that in humans is encoded by the CEBPG gene. The C/EBP family of ... "Entrez Gene: CEBPG CCAAT/enhancer binding protein (C/EBP), gamma". Nishizawa M, Nagata S (1992). "cDNA clones encoding leucine- ...
... an epithelial-stromal interaction in breast tumors mediated by CCAAT/enhancer binding protein beta.Cancer Res.2001;61:2328-34. ... Paracrine activation of WNT/beta-catenin pathway in uterine leiomyoma stem cells promotes tumor growth.Proc Natl Acad Sci USA. ...
... found in inflammatory zone 1/resistin-like molecule alpha gene expression by a STAT6 and CCAAT/enhancer-binding protein- ... Resistin-like beta is a protein that in humans is encoded by the RETNLB gene. GRCh38: Ensembl release 89: ENSG00000163515 - ... 2003). "The Secreted Protein Discovery Initiative (SPDI), a Large-Scale Effort to Identify Novel Human Secreted and ... "Entrez Gene: RETNLB resistin like beta". Kubota T, Kawano S, Chih DY, et al. (2001). "Representational difference analysis ...
"CEBPB CCAAT enhancer binding protein beta [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. National Institutes of ... SUMOylation can affect protein-protein interactions and affect protein ubiquitination. Palmitoylation is the addition of a ... Table 3. Table of WDCP protein Isoforms and Protein Information. The secondary structure of WDCP Protein Isoform 1 consists of ... WDCP Isoform 1 has no transmembrane domains, actin-binding motifs, ER retention motifs, or Golgi transport signals. The protein ...
C/EBP-beta (bahasa Inggris: CCAAT/enhancer-binding protein beta) adalah faktor transkripsi bZIP yang memiliki berkas genetik ... "CEBPB CCAAT/enhancer binding protein (C/EBP), beta [ Homo sapiens ]". Entrez Gene. Diakses tanggal 2010-10-27.. ... C/EBP-beta dapat membentuk kompleks heterdimer dengan protein sejenis seperti C/EBP-alfa, C/EBP-delta, C/EBP-gamma. ... C/EBP-beta menginduksi transkripsi IL-1, IL-6 beserta respon kekebalan dan peradangan, termasuk pada fase akut. ...
"Different regulation of the LXRalpha promoter activity by isoforms of CCAAT/enhancer-binding proteins". Biochemical and ... A critical role for nuclear liver X receptors alpha and beta". The Journal of Biological Chemistry. 277 (35): 31900-8. doi: ... Liver X receptor alpha (LXR-alpha) is a nuclear receptor protein that in humans is encoded by the NR1H3 gene (nuclear receptor ... The liver X receptors, LXRα (this protein) and LXRβ, form a subfamily of the nuclear receptor superfamily and are key ...
... interaction of CCAAT/enhancer-binding protein with elements flanking the ADH2 TATA box". Gene. 90 (2): 271-9. doi:10.1016/0378- ... Hurley TD, Bosron WF, Hamilton JA, Amzel LM (Sep 1991). "Structure of human beta 1 beta 1 alcohol dehydrogenase: catalytic ... Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. GRCh38: ... 2. The primary structure of the gamma 1 protein chain". European Journal of Biochemistry / FEBS. 145 (3): 447-53. doi:10.1111/j ...
Boruk M, Savory JG, Haché RJ (November 1998). "AF-2-dependent potentiation of CCAAT enhancer binding protein beta-mediated ... the heat shock protein 70 (hsp70) and the protein FKBP4 (FK506-binding protein 4). The endogenous glucocorticoid hormone ... "The non-ligand binding beta-isoform of the human glucocorticoid receptor (hGR beta): tissue levels, mechanism of action, and ... Hulkko SM, Wakui H, Zilliacus J (August 2000). "The pro-apoptotic protein death-associated protein 3 (DAP3) interacts with the ...
March 2001). "Dominant-negative mutations of CEBPA, encoding CCAAT/enhancer binding protein-alpha (C/EBPalpha), in acute ... For example, in humans the Hb gene locus is responsible for the Beta-chain protein (HBB) that is one of the two globin proteins ... an aggregate of multiple copies of the same protein, otherwise known as a homomultimeric protein or homooligomeric protein. In ... In fact, the first study reporting a mutant protein inhibiting the normal function of a wild-type protein in a mixed multimer ...
"A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy ... 2003). "Regulation of transforming growth factor-beta signaling by protein inhibitor of activated STAT, PIASy through Smad3". J ... E3 SUMO-protein ligase PIAS4 is one of several protein inhibitor of activated STAT (PIAS) proteins. It is also known as protein ... "Regulation of transforming growth factor-beta signaling by protein inhibitor of activated STAT, PIASy through Smad3". J. Biol. ...
TGF-beta induced apoptosis proteins (TAIP) GATA binding factors (GATA) Ccaat/Enhancer binding protein (CEBP) cAMP-responsive ... "Protein BLAST: search protein databases using a protein query". blast.ncbi.nlm.nih.gov. Retrieved 2020-03-02. "Genomatix - NGS ... element binding proteins (CREB) Vertebrate TATA binding protein factor (VTBP) C5orf46 is largely expressed in salivary glands ... C/EBP homologous protein (CHOP) X-box binding factors (XBBF) TALE homeodomain class recognizing TG motifs (TALE) Human and ...
2003). "Transcriptional regulation of human CYP3A4 basal expression by CCAAT enhancer-binding protein alpha and hepatocyte ... "Analysis of hepatocyte nuclear factor-3 beta protein domains required for transcriptional activation and nuclear targeting". ... sequence-specific DNA binding. • DNA binding. • transcription factor binding. • protein domain specific binding. • RNA ... HNF-3G is a member of the forkheadclass of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional ...
In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... Promoters represent critical elements that can work in concert with other regulatory regions (enhancers, silencers, boundary ... CCAAT boxes are common, as they are in many promoters that lack TATA boxes. In addition, the motifs NRF-1, GABPA, YY1, and ... moderate reduction of beta globin gene transcriptional activity by a novel mutation of the proximal CACCC promoter element". ...
In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... Promoters represent critical elements that can work in concert with other regulatory regions (enhancers, silencers, boundary ... Hobbs K, Negri J, Klinnert M, Rosenwasser LJ, Borish L (December 1998). "Interleukin-10 and transforming growth factor-beta ... CCAAT boxes are common, as they are in many promoters that lack TATA boxes. In addition, the motifs NRF-1, GABPA, YY1, and ...
4.5 Class: beta-Barrel alpha-helix transcription factors. *4.6 Class: TATA binding proteins *4.6.1 Family: TBP ... DNA-binding domain (DBD), which attaches to specific sequences of DNA (enhancer or promoter. Necessary component for all ... I. constitutively active - present in all cells at all times - general transcription factors, Sp1, NF1, CCAAT ... although the consensus binding site for the TATA-binding protein (TBP) is TATAAAA, the TBP transcription factor can also bind ...
"Regulation of the homeodomain CCAAT displacement/cut protein function by histone acetyltransferases p300/CREB-binding protein ( ... matrix attachment region upstream of the T cell receptor beta gene enhancer binds Cux/CDP and SATB1 and modulates enhancer- ... "Regulation of the homeodomain CCAAT displacement/cut protein function by histone acetyltransferases p300/CREB-binding protein ( ... The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It regulates gene expression, ...
Another aspect of the CCAAT binding motif is the CCAAT/enhancer binding proteins (C/EBPs). They are a group of transcription ... This is composed of three alpha-helices separated by two beta strand-loop domains. Similar to NF-YA, NF-YB has been shown to ... Protein specific binding is required for the CCAAT box activation. These proteins are known as CCAAT box binding proteins/CCAAT ... Ramji, Dpiak P.; Foka, Pelagia (10 May 2002). "Review Article: CCAAT/enhancer-binding proteins: structure, function and ...
Transcription factor assessment indicates many potential TATA-binding protein and CCAAT-enhancer-binding proteins sites, along ... of the protein composed of beta sheets. Ligand binding sites are predicted by I-TASSER from positions 377 to 530 in Isoform X1 ... C1orf112 is predicted to interact with a diverse range of proteins, including multiple mitosis-associated proteins. C1orf112 is ... quality-controlled protein-protein association networks, made broadly accessible". Nucleic Acids Research. 45 (D1): D362-D368. ...
"CCAAT/Enhancer-binding Protein Family Members Recruit the Coactivator CREB-binding Protein and Trigger Its Phosphorylation". ... ERICH4 is not predicted to contain beta-sheets. Program analysis in SWISS-Model proposes a tertiary structure for ERICH4 by ... PMID 12857754.CS1 maint: multiple names: authors list (link) Ramji D.P., Foka P. (2002). "CCAAT/enhancer-binding proteins: ... As suggested by the protein's name, glutamate-rich protein 4, the protein is most highly composed of glutamic acid amino acids ...
TFG-TEC binds to the proximal promoter region of the ENO3 gene. Click on genes, proteins and metabolites below to link to ... Peshavaria M, Hinks LJ, Day IN (Nov 1989). "Structure of human muscle (beta) enolase mRNA and protein deduced from a genomic ... activator protein 1 and 2, CCAAT box transcription factor/nuclear factor I, and cyclic AMP. Unlike the other enolase genes, ... and two myocyte-specific enhancer-binding factor 1 boxes. Upstream of the first exon lies a TATA-like box and CpG-rich region, ...
Transcription factors, peroxis proliferator-activated receptor γ (PPARγ) and CCAAT enhancer-binding proteins (C/EBPs) are main ... Choy L, Skillington J, Derynck R (May 2000). "Roles of autocrine TGF-beta receptor and Smad signaling in adipocyte ... cAMP-responsive element binding protein promotes differentiation, while the activation of PPARγ and C/EBPα is also responsive ... T-cell factor/lymphoid enhancer-binding factor (TCF/LEF), GATA2/3, retinoic acid receptor α, and SMAD6/7 don't affect the ...
Ccaat/Enhancer Binding Protein Vertebrate TATA binding protein factor CCAAT binding factors Activator-, mediator- and TBP- ... The beta-folded sheets occur at many of the key domains, including the EGF-domains, kelch domains, and EGF-laminin domains. ... factor 1 RXR heterodimer binding sites GATA binding factors Nuclear receptor subfamily 2 factors Octamer binding protein EGR/ ... proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be ...
CCAAT binding factors, or CCAAT enhancer binding proteins were found. The TMEM251 protein is 169 amino acids in length. The ... 4 O-beta-N-acetylglucosamine attachment sites All post-translational modifications are conserved in vertebrates. Using various ... Transmembrane protein 251, also known as C14orf109 or UPF0694, is a protein that in humans is encoded by the TMEM251 gene. One ... No vertebrate TATA binding protein factors, RNA polymerase transcription factor II B, ...
"Stem-loop binding protein facilitates 3'-end formation by stabilizing U7 snRNP binding to histone pre-mRNA". Mol Cell Biol. 19 ... "U7 snRNA acts as a transcriptional regulator interacting with an inverted CCAAT sequence-binding transcription factor NF-Y". ... SMN2 pre-mRNA splicing corrected by a U7 snRNA derivative carrying a splicing enhancer sequence". Mol Ther. 15 (8): 1479-1486. ... "Double-target antisense U7 snRNAs promote efficient skipping of an aberrant exon in three human beta-thalassemic mutations". ...
... beta-Barrel alpha-helix transcription factors 4.6 Class: TATA binding proteins 4.6.1 Family: TBP 4.7 Class: HMG-box 4.7.1 ... Transcription factors bind to either enhancer or promoter regions of DNA adjacent to the genes that they regulate. Depending on ... Heteromeric CCAAT factors 4.8.1 Family: Heteromeric CCAAT factors 4.9 Class: Grainyhead 4.9.1 Family: Grainyhead 4.10 Class: ... RAV Cdx protein family DNA-binding protein Inhibitor of DNA-binding protein Nuclear receptor, a class of ligand activated ...
"Modulation of DNA binding properties of CCAAT/enhancer binding protein epsilon by heterodimer formation and interactions with ... Spencer E, Jiang J, Chen ZJ (Feb 1999). "Signal-induced ubiquitination of IkappaBalpha by the F-box protein Slimb/beta-TrCP". ... Parry GC, Mackman N (Dec 1997). "Role of cyclic AMP response element-binding protein in cyclic AMP inhibition of NF-kappaB- ... Gerritsen ME, Williams AJ, Neish AS, Moore S, Shi Y, Collins T (Apr 1997). "CREB-binding protein/p300 are transcriptional ...
CCAAT/enhancer binding protein-α (C/EBPα) induces transdifferentiation of B cells into macrophages at high efficiencies and ... for beta-cell maturation) combination (called PNM) can lead to the transformation of some cell types into a beta cell-like ... Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding domain (TAZ) acting as an intracellular ... using the genetic material encoding reprogramming protein factors, recombinant proteins; microRNA, a synthetic, self- ...
... differentiation by activating a p38 delta mitogen-activated protein kinase cascade that targets CCAAT/enhancer-binding protein ... 2002). "Human serum and glucocorticoid-inducible kinase-like kinase (SGKL) phosphorylates glycogen syntheses kinase 3 beta (GSK ... Serine/threonine-protein kinase D3 (PKD3) or PKC-nu is an enzyme that in humans is encoded by the PRKD3 gene. Protein kinase C ... human immunodeficiency virus type 1 Tat protein is associated with an increase in both NF-kappa B binding and protein kinase C ...
Regulation of Id2 expression by CCAAT/enhancer binding protein beta.. Karaya K1, Mori S, Kimoto H, Shima Y, Tsuji Y, Kurooka H ... similar to the mice lacking the CCAAT enhancer binding protein (C/EBP) beta. Here, we show that Id2 is a direct target of C/ ... Oligonucleotide HpxA was used as a positive control for C/EBPβ binding. DNA-protein complexes were separated on a 6% ... which was achieved by using a system utilizing the fusion protein between C/EBPbeta and the ligand-binding domain of the human ...
Its functional capacity is governed by protein interactions and post-translational protein modifications. During early ... Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase ... "Antioxidant-induced nuclear translocation of CCAAT/enhancer-binding protein beta. A critical role for protein kinase A-mediated ... "Antioxidant-induced nuclear translocation of CCAAT/enhancer-binding protein beta. A critical role for protein kinase A-mediated ...
Interleukin-6-dependent DNA-binding protein (IL6DBP); Nuclear factor for IL-6 expression (NF-IL6); Transcription factor 5 (TCF5 ... CCAAT-enhancer binding protein β (C/EBP-β); ... CCAAT/enhancer binding protein beta is expressed in satellite ... CCAAT-enhancer binding protein β (C/EBP-β); Interleukin-6-dependent DNA-binding protein (IL6DBP); Nuclear factor for IL-6 ... Expression pattern of the CCAAT/enhancer-binding proteins C/EBP-alpha, C/EBP-beta and C/EBP-delta in the human placenta. ...
CCAAT/Enhancer-Binding Protein \(\gamma\) Is a Critical Regulator of IL-1\(\beta\)-Induced IL-6 Production in Alveolar ... CCAAT/enhancer-binding protein \(\gamma\) is a critical regulator of IL-1\(\beta\)-induced IL-6 production in alveolar ... CCAAT/Enhancer-Binding Protein \(\gamma\) Is a Critical Regulator of IL-1\(\beta\)-Induced IL-6 Production in Alveolar ... CCAAT/enhancer binding protein \(\gamma\) (C/EBPγ) is a member of the C/EBP family of transcription factors, which lacks known ...
CCAAT/Enhancer binding proteins (C/EBPs) play important roles in the regulation of cell growth and differentiation. This study ... CCAAT-Enhancer-Binding Protein-beta / genetics * CCAAT-Enhancer-Binding Protein-beta / metabolism* ... CCAAT/Enhancer binding proteins (C/EBPs) play important roles in the regulation of cell growth and differentiation. This study ... Expression and function of CCAAT/enhancer binding proteinbeta (C/EBPbeta) LAP and LIP isoforms in mouse mammary gland, tumors ...
CCAAT/Enhancer-Binding Protein \(\gamma\) Is a Critical Regulator of IL-1\(\beta\)-Induced IL-6 Production in Alveolar ... CCAAT/enhancer-binding protein \(\gamma\) is a critical regulator of IL-1\(\beta\)-induced IL-6 production in alveolar ... CCAAT/enhancer binding protein \(\gamma\) (C/EBPγ) is a member of the C/EBP family of transcription factors, which lacks known ... We further provide the evidence that C/EBP\(\gamma\) inhibits IL-6 expression by inhibiting C/EBP\(\beta\) but not NF-\(\kappa ...
The intronless C/EBPβ gene encodes a single mRNA that produces three protein isoforms, C/EBPβ-1, -2, and -3, which share a ... The transcription factor CCAAT/enhancer binding protein (C/EBP)β is critical for normal growth and differentiation of the ... CCAAT/enhancer binding protein beta (C/EBPβ)-2 transforms normal mammary epithelial cells and induces epithelial to mesenchymal ... article{Bundy2003CCAATenhancerBP, title={CCAAT/enhancer binding protein beta (C/EBPβ)-2 transforms normal mammary epithelial ...
A role for CCAAT/enhancer binding protein beta-liver-enriched inhibitory protein in mammary epithelial cell proliferation.: The ... A role for CCAAT/enhancer binding protein beta-liver-enriched inhibitory protein in mammary epithelial cell proliferation.. ... The transcription factor, CCAAT/enhancer binding protein beta (C/EBPbeta), regulates the expression of genes involved in ... Dimerization of the dominant-negative C/EBPbeta-liver-enriched inhibitory protein (LIP) isoform with the C/EBPbeta-liver- ...
Research Topics about ccaat enhancer binding protein beta ... ccaat enhancer binding protein beta. Summary. Summary: A CCAAT- ... ccaat enhancer binding proteins , ccaat enhancer binding protein beta ... ccaat enhancer binding proteins*transcription factors*gene expression regulation*ccaat enhancer binding protein alpha*cell ... A role for CCAAT/enhancer binding protein beta-liver-enriched inhibitory protein in mammary epithelial cell proliferation. C A ...
Human CEBPb(CCAAT/Enhancer Binding Protein Beta) ELISA Kit. Human CEBPb(CCAAT/Enhancer Binding Protein Beta) ELISA Kit ... Should the Mouse CCAAT/Enhancer Binding Protein Beta (CEBPb) ELISA Kit is proven to show malperformance, you will receive a ... Should the Mouse CCAAT/Enhancer Binding Protein Beta (CEBPb) ELISA Kit is proven to show malperformance, you will receive a ... Known also as CCAAT/Enhancer Binding Protein Beta elisa. Alternative names of the recognized antigen: CRP2 ...
CCAAT/Enhancer Binding Protein Beta) ELISA Kit OSCAR DIAGNOSTIC SERVICES PVT. LTD.is an India based Company in Delhi. ... Porcine C/EBP (CCAAT/Enhancer Binding Protein Beta) ELISA Kit Porcine C/EBP (CCAAT/Enhancer Binding Protein Beta) ELISA Kit ... Porcine C/EBP (CCAAT/Enhancer Binding Protein Beta) ELISA Kit » Porcine C/EBP (CCAAT/Enhancer Binding Protein Beta) ELISA Kit ... Porcine C/EBP (CCAAT/Enhancer Binding Protein Beta) ELISA Kit Porcine C/EBP (CCAAT/Enhancer Binding Protein Beta) ELISA Kit ...
CCAAT/enhancer-binding protein beta. CARS. Compensatory anti-inflammatory response syndrome. CRP. C-reactive protein ...
Islet Biology-Beta Cell-Development and Postnatal Growth (36). *. Islet Biology-Beta Cell-Stimulus-Secretion Coupling and ...
The 5′-flanking sequence of the chicken FTO gene contains nine predicted binding sites for CCAAT/enhancer binding protein beta ... Although phosphorylated STAT3 was not detected to directly bind to FTO promoter, it was found to interact with C/EBP beta. Our ... Lipopolysaccharide challenge increased the C/EBP beta binding to FTO promoter in the liver (P , 0.01 for fragment 1, P , 0.05 ... although the protein content of C/EBP beta was not altered. Moreover, injection of LPS resulted in enhanced phosphorylation of ...
CCAAT-enhancer-binding protein-beta‎ (7 В). *. ► Cell cycle proteins‎ (1 К, 251 В) ... Protein (lb); protein (nb); Protéin (su); Protein (hif); 朊 (lzh); بروتين (ar); Protein (br); ပရိုတိန်း (my); 蛋白質 (yue); Белок ( ... प्रोटिन (dty); Prótín (is); Protein (ms); protein (tr); لحمیات (ur); Bielkovina (sk); білок (uk); 蛋白质 (zh-cn); Protein (gsw); ... protein (sco); Уураг (mn); protein (nn); ಪ್ರೋಟೀನ್ (kn); پرۆتین (ckb); protein (en); fehérje (hu); પ્રોટિન (gu); प्रोटिन (new); ...
CCAAT/enhancer-binding protein beta. MQRLVAWDPACLPLPPPPPAFKSMEVANFYYEADCLAAAYGGKAAPAAPP.... unknown. inhibitor. Nuclear ... Heat shock protein HSP 90-alpha. MPEETQTQDQPMEEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELIS.... unknown. Heat shock-related 70 kDa ... Serine/threonine-protein kinase 17B. MSRRRFDCRSISGLLTTTPQIPIKMENFNNFYILTSKELGRGKFAVVRQC.... unknown. Estrogen receptor alpha. ... ATP-binding cassette sub-family G member 2. MSSSNVEVFIPVSQGNTNGFPATASNDLKAFTEGAVLSFHNICYRVKLKS.... unknown. inhibitor. Solute ...
Antioxidant-induced nuclear translocation of CCAAT/enhancer-binding protein beta. A critical role for protein kinase A-mediated ... and purification of a trefoil peptide motif in a beta-galactosidase fusion protein and its use to search for trefoil-binding ... Immunoprecipitation and characterization of a binding protein specific for the peptide, intestinal trefoil factor. ... Leukemic HRX fusion proteins inhibit GADD34-induced apoptosis and associate with the GADD34 and hSNF5/INI1 proteins. ...
CCAAT-Enhancer-Binding Protein-beta / physiology* * Calcitriol / pharmacology* * Cell Differentiation / drug effects ... isoforms beta-1 and beta-3 were expressed at levels comparable to 1,25D-sensitive HL60-G cells, but isoform beta-2 was ... Treatment of sensitive HL60 cells with 1,25D resulted in predominantly nuclear localization of C/EBP isoforms beta-2 and beta-3 ...
... quantification of relative binding affinities and rapid protein classification, all independently of the transactivation ... Interactions are detected and quantified by laser scanning to reveal proteins that differentially bind to nonmodified or ... G9a-mediated lysine methylation alters the function of CCAAT/enhancer-binding protein-beta. J. Biol. Chem. 283, 26357-26363 ( ... Edmondson, D.G. & Roth, S.Y. Identification of protein interactions by far western analysis. Curr. Protoc. Protein Sci. 19, ...
... protein_coding gene_symbol:Cebpb description:CCAAT/enhancer binding protein (C/EBP), beta [Source:MGI Symbol;Acc:MGI:88373] ... cdna chromosome:GRCm38:2:167688915:167690418:1 gene:ENSMUSG00000056501 gene_biotype:protein_coding transcript_biotype: ... CCAAT/enhancer binding protein (C/EBP), beta. 105. 22. 7. 18. Sequence references in MGI J:91388 Mouse Genome Informatics ...
... activity and E2 protein in liver tissue due to the ,i,Dbt,sup,tm1Geh,/sup, ,/i, knock-out mutation is rescued by the two ... Cebpb, CCAAT/enhancer binding protein beta, rat. Expressed Gene. tTA, tetracycline-controlled transactivator, E. coli. ... Levels of the human E2 protein are approximately equal to mouse E2 protein in livers of control mice. However, when crossed to ... despite nearly equal protein levels, indicating suboptimal BCKDH activity of protein assembled with human E2 ...
CCAAT-enhancer-binding protein homologous protein; JNK, c-Jun N-terminal kinase; GSK 3β, Glycogen synthase kinase 3 beta; IRS-1 ... CCAAT-Enhancer-Binding Protein Homologous Protein; Cpg-Odns, Cpg Oligodeoxynucleotides; CrNano, Chromium Nanoparticles; CRP, C- ... The cellular metabolism is regulated by various proteins (e.g., protein kinase C), receptors (e.g., receptor tyrosine kinase) ... where it binds to the receptor within the cell and blocks the endogenous hormone from binding. The normal signaling is blocked ...
CCAAT/enhancer binding protein (C/EBP), beta MGI:88373 .yui-skin-sam .yui-dt th{ background:url(http://www.informatics.jax.org/ ...
1992) A member of the C/EBP family, NF-IL6 beta, forms a heterodimer and transcriptionally synergizes with NF-IL6. Proc. Natl. ... Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ... Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ... Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ...
The roles of phosphatidylinositol 3-kinase and CCAAT/enhancer-binding protein beta. J Biol Chem. 2005;280(39):33240-9.PubMed ... Johnson GL, Lapadat R. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science. 2002; ... The effect of vitamin E and beta carotene on the incidence of lung cancer and other cancers in male smokers. N Engl J Med. 1994 ... Beta-catenin, twist and snail: transcriptional regulation of EMT in smokers and COPD, and relation to airflow obstruction. Sci ...
0 (CCAAT-Enhancer-Binding Protein-beta); 0 (CD11c Antigen); 0 (GKLF protein); 0 (Kruppel-Like Transcription Factors); 0 ( ... Prote na beta Intensificadora de Liga o a CCAAT/defici ncia. Prote na beta Intensificadora de Liga o a CCAAT/gen tica. Ant geno ... Interferon beta/defici ncia. Interferon beta/gen tica. Interferon beta/imunologia. Lipopolissacar deos/imunologia. Ativa o ... 0 (CD11c Antigen); 0 (Immunoglobulin J Recombination Signal Sequence-Binding Protein); 0 (Interleukin-17); 0 (Rbpj protein, ...
CCAAT/enhancer binding protein beta; Ccl12, chemokine ligand 12; CXCL3, chemokine (C-X-C motif) ligand 3; IL, interleukin. The ... 1995). Expression of monocyte chemoattractant protein-1 and macrophage inflammatory protein-1 after focal cerebral ischemia in ... Detected elevated expression of SMAD5 protein may be associated with the activation of the bone morphogenetic protein (BMP) ... The miRNAs bind to their mRNA target at complementary sequences and downregulate gene expression by inhibiting the mRNA ...
... and noradrenaline induce the transcription factors CCAAT/enhancer binding protein (C/EBP)-beta and C/EBP delta in mouse ... and noradrenaline induce the transcription factors CCAAT/enhancer binding protein (C/EBP)-beta and C/EBP delta in mouse ... and noradrenaline induce the transcription factors CCAAT/enhancer binding protein (C/EBP)-beta and C/EBP delta in mouse ... and noradrenaline induce the transcription factors CCAAT/enhancer binding protein (C/EBP)-beta and C/EBP delta in mouse ...
Regulation of CCAAT/enhancer binding protein-alpha gene transcription by interleukin-6. Atherosclerosis Supplements 5(1), pp. ... Signalling pathways underlying transforming growth factor-beta regulated expression of key genes implicated in the control of ... CCAAT/Enhancer binding proteins: structure, function and regulation. Biochemical Journal 365, pp. 561-575. (10.1042/BJ20020508) ... Regulation of CCAAT/enhancer binding protein-alpha gene transcription by interleukin-6. Atherosclerosis Supplements 5(1), pp. ...
5′ AMP-activated protein kinase. CEBPB. CCAAT/enhancer-binding protein beta. G6Pase. Glucose 6-phosphatase ... Zheng S, Rollet M, Pan YX (2011) Maternal protein restriction during pregnancy induces CCAAT/enhancer-binding protein (C/EBPβ) ... Petrik J, Reusens B, Arany E, Remacle C, Coelho C, Hoet JJ, Hill DJ (1999) A low protein diet alters the balance of islet cell ... Bellinger L, Lilley C, Langley-Evans SC (2004) Prenatal exposure to a maternal low-protein diet programmes a preference for ...
  • Should the Mouse CCAAT/Enhancer Binding Protein Beta (CEBPb) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement. (bioemm.com)
  • Description: A sandwich quantitative ELISA assay kit for detection of Mouse CCAAT/Enhancer Binding Protein Beta (CEBPb) in samples from tissue homogenates, cell lysates or other biological fluids. (bioemm.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human CCAAT/Enhancer Binding Protein Beta (CEBPb) in Tissue homogenates, cell lysates and other biological fluids. (bioemm.com)
  • CCAAT/enhancer-binding protein beta ) adalah faktor transkripsi bZIP yang memiliki berkas genetik CEBPB pada kromosom 20 lokasi 20q13.1, yang tidak dilengkapi dengan intron . (wikipedia.org)
  • CCAAT/enhancer-binding protein beta is a protein that in humans is encoded by the CEBPB gene. (wikipedia.org)
  • Genes coding for transporter proteins that confer multidrug resistance to the cells have also been found to be activated by CEBPB. (wikipedia.org)
  • The transcription factor binding sites associated with this promoter and confirmed with a ChIP signal include HNF4A, CEBPB, ERG1, FOS1, ETS1, and E2F6. (wikipedia.org)
  • CEBPB is an intronless gene and its protein is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. (prospecbio.com)
  • CEBPB antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. (prospecbio.com)
  • Human CEBPB partial ORF ( NP_005185, 256 a.a. - 344 a.a.) recombinant protein with GST-tag at N-terminal. (abnova.com)
  • Focusing in on specific genes, Kaleta and colleagues found that those whose expression changed across the lifespan frequently contained binding sites for the transcription factors E2F1, NFAT, CEBPB, AP1, suggesting these as master regulators of aging-related processes. (alzforum.org)
  • These sequences represent the protein coding region of the CEBPB cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)
  • CCAAT/enhancer-binding protein beta (CEBPB), also known as LAP, is a important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. (ptglab.com)
  • CEBPb mRNAs possess alternative translation-initiation codons, which result in the formation of truncated forms of the protein. (ptglab.com)
  • Mouse polyclonal antibody raised against a full-length human CEBPB protein. (abnova.com)
  • CEBPB (AAH21931.1, 1 a.a. ~ 345 a.a) full-length human protein. (abnova.com)
  • CEBPA (CCAAT Enhancer Binding Protein Alpha) is a Protein Coding gene. (genecards.org)
  • Reciprocally E2F1 blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. (genecards.org)
  • 2015. Protein Kinase C is involved in the induction of ATP-Binding cassette transporter A1 expression by liver X receptor/retinoid X receptor agonist in human macrophages . (cardiff.ac.uk)
  • The function of the protein is not completely understood, but WDCP has been identified in a fusion protein with anaplastic lymphoma kinase found in colorectal cancer. (wikipedia.org)
  • WDCP protein domains include two tryptophan-aspartic acid repeat sites, multiple phosphorylation sites, and a domain that interacts with the hemopoietic cell kinase. (wikipedia.org)
  • for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). (wikiversity.org)
  • Studies on the mechanisms underlying these changes have unraveled the involvement of mitogen-activated protein kinase (MAPK) pathway in the disease process. (springer.com)
  • For instance, the disruption of calcineurin, a Ca 2+ -dependent phosphatase, or Ca 2+ /calmodulin-dependent protein kinase II or IV, two Ca 2+ -dependent kinases, profoundly impairs β-cell function, likely by modulating the activity of Ca 2+ -responsive transcription factors such as NFAT, CREB, and TORC2 ( 5 - 9 ). (diabetesjournals.org)
  • Protein limitation in vivo or amino acid deprivation of cells in culture causes a signal transduction cascade consisting of activation of the kinase GCN2 (general control nonderepressible 2), phosphorylation of eukaryotic initiation factor 2, and increased synthesis of activating transcription factor (ATF) 4 by a translational control mechanism. (semanticscholar.org)
  • A mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK)-dependent transcriptional program controls activation of the early growth response 1 (EGR1) gene during amino acid limitation. (semanticscholar.org)
  • RNA-binding protein Musashi1 modulates glioma cell growth through the post-transcriptional regulation of Notch and PI3 kinase/Akt signaling pathways. (peprotech.com)
  • Phosphorylation of ATF1 at Ser63 by PKA (cAMP-dependent protein kinase) and related kinases was the only known post-translational regulatory mechanism of ATF1. (biologists.org)
  • Here, we found that HIPK2 (homeodomain-interacting protein kinase 2), a DNA-damage-responsive nuclear kinase, is a new ATF1 kinase that phosphorylates Ser198 but not Ser63. (biologists.org)
  • Stimulus-coupled activation of ATF1 is induced by growth factors as well as stress-inducing agents, in which ATF1 is phosphorylated at Ser63 located in the kinase-inducible (KID) domain by PKA (cAMP-dependent protein kinase) and several other serine-threonine (Ser-Thr) kinases ( Mayr and Montminy, 2001 ). (biologists.org)
  • To better understand the ATF1-mediated ARE regulation, in this study we attempted to find ATF1-interacting proteins by yeast two-hybrid screening and have identified homeodomain-interacting protein kinase 2 (HIPK2) as an ATF1 binding protein. (biologists.org)
  • In this study, we show that EGFR activation promoted GBP1 expression in GBM cell lines through a signaling pathway involving Src and p38 mitogen-activated protein kinase. (rupress.org)
  • Mice deficient for Id2, a negative regulator of basic helix-loop-helix (bHLH) transcription factors, exhibit a defect in lactation due to impaired lobuloalveolar development during pregnancy, similar to the mice lacking the CCAAT enhancer binding protein (C/EBP) beta. (nih.gov)
  • The CCAAT/enhancer (C/EBP) family of basic-leucine zipper (bZIP) transcription factors is a multifaceted highly-regulated system for gene regulation. (springer.com)
  • CCAAT/enhancer binding protein \(\gamma\) (C/EBPγ) is a member of the C/EBP family of transcription factors, which lacks known activation domains. (harvard.edu)
  • C/EBP proteins are all members of the b-ZIP family of transcription factors and share a highly homologous carboxy terminus that contains the basic and leucine zipper protein domains. (asm.org)
  • CCAAT/enhancer binding proteins (C/EBPs) are six-member family (α to ζ) of transcription factors. (nature.com)
  • Myocyte enhancer factor-2 (MEF2) proteins are a family of transcription factors which through control of gene expression are important regulators of cellular differentiation and consequently play a critical role in embryonic development. (wikiversity.org)
  • The serum response factor is a member of the MADS-box ( M CM1, A gamous, D eficiens, and S RF) box superfamily of transcription factors, [5] binding to the serum response element (SRE) in the promoter region of target genes. (wikiversity.org)
  • CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that regulate cell growth and differentiation in numerous cell types. (bloodjournal.org)
  • The CCAAT/enhancer-binding protein (C/EBP) family falls into this category of transcription factors, with many physiologic and pathologic conditions associated with their activities. (bloodjournal.org)
  • 1 , 2 To date, 6 C/EBP family members have been identified, with further diversity achieved by the generation of different isoforms and extensive protein-protein interactions both within the family and with other transcription factors. (bloodjournal.org)
  • Work over the past 15 years has provided increasing evidence for a role of gene expression in drug addiction, as several transcription factors-proteins that bind to specific response elements in the promoter regions of target genes and regulate those genes' expression-have been implicated in drug action. (royalsocietypublishing.org)
  • These Fos family proteins heterodimerize with Jun family proteins (c-Jun, JunB or JunD) to form active activator protein-1 (AP-1) transcription factors that bind to AP-1 sites (consensus sequence: TGAC/GTCA) present in the promoters of certain genes to regulate their transcription. (royalsocietypublishing.org)
  • One of these classes consists of transcription factors, which are known to bind to DNA and control the transcription activities of their target genes. (hindawi.com)
  • CCAAT/enhancer-binding protein β (C/EBPβ), is a member of a family of transcription factors consisting of six structurally related basic leucine-zipper DNA-binding proteins. (biomedcentral.com)
  • Proteins containing this domain are transcription factors. (genenames.org)
  • The CCAAT/enhancer binding protein family (C/EBP) are transcription factors that play integral roles in the development and function of many organ systems, including hematopoietic cells, adipose tissues, and liver. (zfin.org)
  • Translocation of C/EBPbeta into the nucleus, which was achieved by using a system utilizing the fusion protein between C/EBPbeta and the ligand-binding domain of the human estrogen receptor (C/EBPbeta-ERT), demonstrated the rapid induction of endogenous Id2 expression. (nih.gov)
  • In reporter assays, transactivation of the Id2 promoter by C/EBPbeta was observed and, among three potential C/EBPbeta binding sites found in the 2.3 kb Id2 promoter region, the most proximal element was responsible for the transactivation. (nih.gov)
  • Electrophoretic mobility shift assay (EMSA) identified this element as a core sequence to which C/EBPbeta binds. (nih.gov)
  • The "full-length" 38 kd C/EBPbeta LAP ("Liver-enriched Activator Protein") isoform is the predominant C/EBPbeta protein isoform in mammary tumor whole cell lysates, however, the truncated 20 kd C/EBPbeta LIP ("Liver-enriched Inhibitory Protein") isoform is also present at detectable levels (mean LAP:LIP ratio 5.3:1). (nih.gov)
  • C/EBPbeta LIP overexpressing HC11 cells did not express beta-casein mRNA (mammary epithelial cell differentiation marker) in response to lactogenic hormones. (nih.gov)
  • These results demonstrate that the "full-length" C/EBPbeta LAP isoform is the predominant C/EBPbeta protein isoform expressed in mouse mammary gland in vivo and mouse mammary epithelial cell cultures in vitro. (nih.gov)
  • Ectopic overexpression of C/EBPbeta LIP (LAP:LIP ratio of approximately 1:1) inhibits mammary epithelial cell differentiation (beta-casein expression). (nih.gov)
  • The transcription factor, CCAAT/enhancer binding protein beta (C/EBPbeta), regulates the expression of genes involved in proliferation and terminal differentiation. (mysciencework.com)
  • Dimerization of the dominant-negative C/EBPbeta-liver-enriched inhibitory protein (LIP) isoform with the C/EBPbeta-liver-enriched activating protein (LAP) isoform inhibits the transcriptional activation of genes involved in differentiation. (mysciencework.com)
  • Functional RNAi screening on a set of these ALK transcriptional targets revealed that the transcription factor C/EBPbeta and the antiapoptotic protein BCL2A1 are absolutely necessary to induce cell transformation and/or to sustain the growth and survival of ALK-positive ALCL cells. (nih.gov)
  • C/EBPbeta is an upstream gene that regulates WT1 expression by binding to the novel enhancer region. (genscript.com)
  • The increase of nuclear C/EBPbeta protein was dependent on p38. (genscript.com)
  • Kim JW, Tang QQ, Li X, Lane MD (2007) Effect of phosphorylation and S-S bond-induced dimerization on DNA binding and transcriptional activation by C/EBPbeta. (phosphosite.org)
  • The inner nuclear membrane protein emerin regulates beta-catenin activity by restricting its accumulation in the nucleus. (wikipathways.org)
  • Xu X, Hu J, McGrath B, Cavener D. GCN2 regulates the CCAAT enhancer binding protein beta and hepatic gluconeogenesis. (labome.org)
  • The results of high throughput expression microarray and chromatin occupancy analyses reveal that Pdx1 regulates a broad array of genes involved in diverse functions of the ER, including proper disulfide bond formation, protein folding, and the unfolded protein response. (pnas.org)
  • Regulation of Id2 expression by CCAAT/enhancer binding protein beta. (nih.gov)
  • Induction of endogenous Id2 expression by the C/EBPβ-ERT fusion protein. (nih.gov)
  • The nuclear factor for IL-6 expression (NF-IL6) was discovered as a member of the CCAAT-enhancer binding proteins (C/EBP) in 1990, from which it derived its current name: C/EBP-β (Akira et al. (springer.com)
  • 1990 ). C/EBP-β was first found to bind to an interleukin 1 (IL1) responsive element necessary for IL-6 expression (Akira et al. (springer.com)
  • Expression pattern of the CCAAT/enhancer-binding proteins C/EBP-alpha, C/EBP-beta and C/EBP-delta in the human placenta. (springer.com)
  • Chakrabarty A, Roberts MR. Ets-2 and C/EBP-beta are important mediators of ovine trophoblast Kunitz domain protein-1 gene expression in trophoblast. (springer.com)
  • Lala-Tabbert N, Fu D, Wiper-Bergeron N. Induction of CCAAT/enhancer-binding protein beta expression with the phosphodiesterase inhibitor isobutylmethylxanthine improves myoblast engraftment into dystrophic muscle. (springer.com)
  • Marchildon F, Lamarche E, Lala-Tabbert N, St-Louis C, Wiper-Bergeron N. Expression of CCAAT/enhancer binding protein beta in muscle satellite cells inhibits myogenesis in cancer cachexia. (springer.com)
  • Importantly, we demonstrate for the first time that C/EBP\(\gamma\) plays a critical role in regulating IL-1\(\beta\)-induced IL-6 expression in both mouse primary alveolar type II epithelial cells and a lung epithelial cell line, MLE12. (harvard.edu)
  • We further provide the evidence that C/EBP\(\gamma\) inhibits IL-6 expression by inhibiting C/EBP\(\beta\) but not NF-\(\kappa\)B stimulatory activity in MLE12 cells. (harvard.edu)
  • This defect in beta-casein expression was not corrected by carrying out the differentiation protocol in the presence of an artificial extracellular matrix. (nih.gov)
  • Our results reveal that FTO expression in liver, but not in hypothalamus, is affected by the i.p. injection of LPS, which may be mediated through tissue-specific FTO transcriptional regulation by C/EBP beta and STAT3 interaction. (biomedcentral.com)
  • Takayama, S. & Reed, J.C. Protein interaction cloning by far-Western screening of lambda-phage cDNA expression libraries. (nature.com)
  • The E2-targeted mutation leads to absence of branched-chain keto acid dehydrogenase (BCKDH) activity and E2 protein in liver tissue, but this absence is rescued by the two transgenes: liver-directed expression of the modified human BCKDH E2 subunit from the complimentary "Tet-off" transgenes abrogates the severity of Maple Syrup Urine Disease (MSUD) phenotype observed in E2-deficient single mutant mice. (jax.org)
  • While C/EBP proteins are expressed in many human tissues, high levels of C/EBP mRNA and protein expression are limited to only a few cell types, including cells of the myeloid lineage. (asm.org)
  • In fact, C/EBP proteins are intimately involved in the regulation of myelocytic/monocytic gene expression. (asm.org)
  • 2016. The role of mitogen-activated protein kinases and sterol receptor coactivator-1 in TGF-β-regulated expression of genes implicated in macrophage cholesterol uptake . (cardiff.ac.uk)
  • In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. (wikipedia.org)
  • Observational work has shown that expression of CEBP-beta in blood leukocytes is positively associated with muscle strength in humans, emphasizing the importance of the immune system, and particularly macrophages, in the maintenance of muscle function. (wikipedia.org)
  • Consistent with these findings, L-165,041 increased 14-3-3ε mRNA and protein level, whereas PPARδ small interfering RNA suppressed both basal and L-165,041-dependent YWHAE transcription and 14-3-3ε protein expression. (ahajournals.org)
  • Intriguingly, activation or knock down of endogenous PPARδ regulated C/EBPβ protein expression. (ahajournals.org)
  • The 3 isotypes identified in vertebrates, PPARα (NR1C1), PPARδ (NR1C2), and PPARγ (NR1C3), regulate target gene expression by binding to specific PPAR response elements (PPREs) as a heterodimer with a retinoid X receptor. (ahajournals.org)
  • The CCAAT enhancer binding protein beta (C/EBP-β) increased the expression of miR-16 after I/R injury. (nature.com)
  • The ChIP and luciferase promoter assay indicated that about −1.0 kb to −0.5 kb upstream of miR-16 genome promoter region containing C/EBP-β binding motif transcriptionally regulated miR-16 expression. (nature.com)
  • WDCP exhibits increased protein expression in endocrine tissues, and well as the kidney and urinary bladder. (wikipedia.org)
  • Arabidopsis SWI/SNF chromatin remodeling complex binds both promoters and terminators to regulate gene expression. (abcam.com)
  • TSA and BIX-01294 Induced Normal DNA and Histone Methylation and Increased Protein Expression in Porcine Somatic Cell Nuclear Transfer Embryos. (abcam.com)
  • Each phase of EO is accompanied by a change in cell shape or cell arrangement [ 3 , 4 ] (Figure 1 ) and the expression of a specific protein repertoire. (biomedcentral.com)
  • Elevated ATF4 expression, in the absence of other signals, is sufficient for transcriptional induction via CCAAT enhancer-binding protein-activating transcription factor response elements. (semanticscholar.org)
  • Tissue- and stage-specific expression, as well as variable DNA-binding specificities, contributes to the differences in the biologic functions of the C/EBP isoforms. (bloodjournal.org)
  • Since ΔFosB is a highly stable protein, it represents a mechanism by which drugs produce lasting changes in gene expression long after the cessation of drug use. (royalsocietypublishing.org)
  • Insulin gene expression is principally controlled by a highly conserved region lying approximately 340bp upstream of the transcription initiation start, termed the enhancer/promoter control region [14,15]. (diabetesincontrol.com)
  • Data show that CCAAT/enhancer-binding protein beta (C/EBP beta) expression is increased in endothelial cells and retinal pigment epithelial cells infected by Toxoplasma gondii, resulting in the activation of autophagy in host cells by inhibiting mechanistic target of rapamycin protein (mTOR) pathway. (genscript.com)
  • Previous studies have shown that CCAAT/enhancer-binding protein α (C/EBPα) plays a very important role during adipocyte terminal differentiation and that AP-2α (activator protein 2α) acts as a repressor to delay the expression of C/EBPα. (asm.org)
  • Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. (genecards.org)
  • To regulate gluconeogenesis, functionally cooperates with FOXO1 binding to IRE-controlled promoters and regulating the expression of target genes such as PCK1 or G6PC. (genecards.org)
  • Longer time control involves transcriptional regulation that affects the expression levels of key proteins. (mdpi.com)
  • Depletion of JunB by small interfering ribonucleic acid abrogates TGF-β-induced disruption of cell-cell junctions, formation of actin fibers, focal adhesions, and expression of fibrotic proteins. (biomedsearch.com)
  • Western blot, ELISA and immunohistochemistry were used to confirm the protein expression of genes of interest. (biomedcentral.com)
  • Administration of PROG significantly down-regulated three of the 13 increased target genes after TBI (Ccl-2, IL-1b and Cxcl-10), but did not inhibit the expression of any of the detected TLRs and adaptor/interacting proteins. (biomedcentral.com)
  • Rather, PROG up-regulated the expression of one TLR (TLR9), 5 adaptor/interacting proteins, 5 effectors and 10 downstream target genes. (biomedcentral.com)
  • We confirmed that Ccl-2, Cxcl-10, TLR2 and TLR9 proteins were expressed in brain tissue, a finding consistent with our observations of mRNA expression. (biomedcentral.com)
  • Both the Smad and p38 MAPK pathways play a crucial role in Runx2 expression following induction by transforming growth factor-beta and bone morphogenetic protein. (wikipathways.org)
  • Interleukin (IL)-6 gene expression in the central nervous system is necessary for fever response to lipopolysaccharide or IL-1 beta: a study on IL-6-deficient mice. (google.it)
  • Developmental control of proliferation relies on tight regulation of protein expression. (xenbase.org)
  • NPM-ALK-dependent expression of the transcription factor CCAAT/enhancer binding protein beta in ALK-positive anaplastic large cell lymphoma Blood. (usc.edu)
  • In this study, AGAF was examined for the granulocyte colony-stimulating factor (G-CSF) production and related protein expression in RAW 264.7 murine macrophages. (hindawi.com)
  • To accomplish this, we established a novel homozygous mouse model overexpressing rIAPP at a comparable expression rate and, on the same background, as a homozygous transgenic hIAPP mouse model previously reported to develop diabetes associated with beta-cell loss. (mendeley.com)
  • Both rIAPP and hIAPP transgenic mice have increased expression of BiP, but only hIAPP transgenic mice have elevated ER stress markers (X-box-binding protein-1, nuclear localized CCAAT/enhancer binding-protein homologous protein, active caspase-12, and accumulation of ubiquitinated proteins). (mendeley.com)
  • Enhancer transcription reveals subtype-specific gene expression programs controlling breast cancer pathogenesis. (mdanderson.org)
  • The intronless C/EBPβ gene encodes a single mRNA that produces three protein isoforms, C/EBPβ-1, -2, and -3, which share a common basic-leucine zipper domain at their C-terminus, but are distinguished at their N-termini by the in-frame methionine codon used to initiate translation. (semanticscholar.org)
  • Table of WDCP protein Isoforms and Protein Information. (wikipedia.org)
  • The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. (genscript.com)
  • The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. (genecards.org)
  • In the Id2 promoter region spanning positions −2248 to +84 from the transcription initiation site, there are three potential C/EBPβ binding sites, named CβE1 (−445 to −436), CβE2 (−81 to −73) and CβE3 (−73 to −65), as indicated by arrows in the schematic representation of pGL2-Id2/A. The numbers shown on the left of the respective reporter constructs indicate the positions from the transcription initiation site. (nih.gov)
  • Mechanism of c-Myb-C/EBP beta cooperation from separated sites on a promoter. (springer.com)
  • However, previous study demonstrates that C/EBP\(\gamma\) augments the C/EBP\(\beta\) stimulatory activity in lipopolysaccharide induction of IL-6 promoter in a B lymphoblast cell line. (harvard.edu)
  • Although phosphorylated STAT3 was not detected to directly bind to FTO promoter, it was found to interact with C/EBP beta. (biomedcentral.com)
  • These mice were created by Dr. Hermann Bujard (Universitat Heidelberg) to express tetracycline-controlled transactivator (tTA) under control of the rat liver-enriched activator protein promoter. (jax.org)
  • Thus, HIV-1 LTR ATF/CREB binding site sequence variation may modulate cellular signaling at the viral promoter through the C/EBP pathway. (asm.org)
  • The promoter elements of many monocyte-specific genes contain C/EBP binding sites, including macrophage inflammatory protein 1 alpha, tumor necrosis factor alpha ( 32 ), IL-6 ( 6 , 27 , 38 ), and IL-8 ( 27 , 36 ). (asm.org)
  • Surprisingly, PPAR response elements in YWHAE promoter were not required for upregulation by PPARδ, whereas a CCAAT/enhancer binding protein (C/EBP) site located at −160/−151 bp regulated both basal and PPARδ-dependent promoter activity. (ahajournals.org)
  • CCAAT/Enhancer binding proteins (C/EBPs) play important roles in the regulation of cell growth and differentiation. (nih.gov)
  • A 76bp fragment conferred a 20-fold induction by SA in transgenic tobacco plants and protein binding studies indicated that the core sequence of this SARE is TTCGACCTCC [40]. (wikiversity.org)
  • Induction of endoplasmic reticulum stress-induced beta-cell apoptosis and accumulation of polyubiquitinated proteins by human islet amyloid polypeptide. (mendeley.com)
  • Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. (uniprot.org)
  • The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. (wikipedia.org)
  • Here, we show that β-cells in Abcc8 −/− mice exhibit chronic membrane depolarization and a sustained elevation in [Ca 2+ ] i and dysregulation of more than 4,200 genes, many of which are involved in cell adhesion, Ca 2+ binding and Ca 2+ signaling, and maintenance of β-cell identity. (diabetesjournals.org)
  • Functional analysis revealed a previously unknown link between C/EBP proteins and circadian clock genes. (bloodjournal.org)
  • Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. (genscript.com)
  • This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. (genecards.org)
  • Binds directly to the consensus DNA sequence 5-T[TG]NNGNAA[TG]-3 acting as an activator on distinct target genes (PubMed:11242107). (genecards.org)
  • MiRNAs constitute an essential component of gene regulation and control well over half of all protein coding genes in humans [ 6 , 7 ]. (hindawi.com)
  • NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. (ptglab.com)
  • WDCP protein isoform 1 is 721 amino acids in length. (wikipedia.org)
  • The protein sequence for WDCP Protein Isoform 1 is shown below. (wikipedia.org)
  • Protein sequence of WDCP protein isoform 1. (wikipedia.org)
  • Transcriptional activators include C/EBP α ( 4 ), C/EBP β (nuclear factor interleukin-6), (IL-6) ( 2 , 9 , 12 , 40 ), C/EBP δ ( 42 ), C/EBP ɛ ( 44 ), and C/EBP-related protein 1 (CRP-1) ( 42 ). (asm.org)
  • A unique synaptic activity-responsive element (SARE) sequence, composed of the consensus binding sites for SRF, MEF2 and CREB, is necessary for control of transcriptional upregulation of the Arc gene in response to synaptic activity. (wikiversity.org)
  • Transcriptional regulation of adipocyte differentiation: a central role for CCAAT/enhancer-binding protein (C/EBP) β. (wikipathways.org)
  • The signaling pathway of G-CSF production involves AGAF and mitogen-activated protein kinases (MAPKs) inhibitors and pattern-recognition receptor antibodies. (hindawi.com)
  • Podust LM, Krezel AM, Kim Y. Crystal structure of the CCAAT box/enhancer-binding protein beta activating transcription factor-4 basic leucine zipper heterodimer in the absence of DNA. (springer.com)
  • The Basic Leucine Zipper Domain ( bZIP domain ) is found in many DNA binding eukaryotic proteins. (genenames.org)
  • It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. (wikipedia.org)
  • mice unable to express CEBP-beta have macrophages that cannot differentiate (specialize) and thus are unable to perform all their biological functions - including macrophage-mediated muscle repair. (wikipedia.org)
  • The transcription factor CCAAT/enhancer binding protein (C/EBP)β is critical for normal growth and differentiation of the mammary gland. (semanticscholar.org)
  • C/EBP proteins play a key role in regulating proliferation and differentiation of many cell types including mammary epithelia cells, neuronal cells, granulocytes, hepatocytes, and adipocytes. (bloodjournal.org)
  • One of the differentiation-inducing agents, all-trans retinoic acid (ATRA), which can induce granulocytic differentiation in myeloid leukemic cell lines, has been introduced into clinics to treat patients with acute promyelocytic leukemia (APL) in which a PML-RARA fusion protein is generated by a t(15;17)(q22;q12) chromosomal translocation. (mdpi.com)
  • Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. (peprotech.com)
  • 2009) O-linked N-acetylglucosamine modification on CCAAT enhancer-binding protein beta: role during adipocyte differentiation. (phosphosite.org)
  • In this study, we demonstrate that C/EBP\(\gamma\) activation was induced by IL-1\(\beta\) treatment in lung epithelial cells. (harvard.edu)
  • [1] C/EBP-beta dapat membentuk kompleks heterdimer dengan protein sejenis seperti C/EBP-alfa , C/EBP-delta , C/EBP-gamma . (wikipedia.org)
  • The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. (genscript.com)
  • The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. (genecards.org)
  • We have identified and characterized putative zebrafish orthologs of mammalian C/EBP alpha, beta, gamma, and delta using low-stringency hybridization screening and computer searches of the GenBank EST database. (zfin.org)
  • The protein encoded by this intronless gene is a bZIP transcription factor that can bind as a homodimer to certain DNA regulatory regions. (wikipedia.org)
  • Y. pestis exerts YopJ-dependent suppression of tumor necrosis factor alpha secretion and phosphorylation of mitogen-activated protein kinases and thus resembles enteropathogenic Yersinia . (asm.org)
  • In these triple mutant mice the absence of keto acid dehydrogenase (BCKDH) activity and E2 protein in liver tissue due to the Dbt tm1Geh knock-out mutation is rescued by the two transgenes, rendering this strain as a useful model for Maple Syrup Urine Disease (MSUD). (jax.org)
  • Because these mice have near normal amounts of E2 protein, but only 5-6% of normal BCKDH enzyme activity, it is probable that the c-myc tag at the carboxy-terminus of the human E2 transgene interferes with enzymatic activity. (jax.org)
  • In order to determine the importance of RXRα AF-1 domain A/B during development in vivo, we have engineered a mouse mutant line that expresses a truncated RXRα protein (RXRαΔA/B). The phenotypic analysis of mice carrying this mutation indicate that RXRα AF-1 domain A/B is important for transducing RA signals in vivo. (biologists.org)
  • Twenty-four hours post-surgery, mice were killed and peri-contusional brain tissue was harvested for genomic detection and protein measurement. (biomedcentral.com)
  • Lymphoproliferative disorder and imbalanced T‐helper response in C/EBP beta‐deficient mice. (google.it)
  • Activity-based protein profiling reveals mitochondrial oxidative enzyme impairment and restoration in diet-induced obese mice. (labome.org)
  • Researchers at the University of Illinois at Urbana-Champaign report that experiments involving mice - to be detailed in the Proceedings of the National Academy of Sciences - indicate that the transcription factor protein C/EBPb must be present in the uterus for pregnancy to occur. (breakingnewsblog.com)
  • In normal conditions, the protein, driven mostly by progesterone, is expressed rapidly and in large quantities during the critical four-day implantation period in mice, Bagchi said. (breakingnewsblog.com)
  • After messenger RNA profiling zeroed in on C/EBPb's activity, the researchers collaborated with Peter F. Johnson of the National Cancer Institute's Laboratory of Protein Dynamics and Signaling, who created mice that lacked the protein. (breakingnewsblog.com)
  • The experimental mice were then used to observe the relationships of the hormones and their receptors with the protein under varying conditions during the critical implantation period. (breakingnewsblog.com)
  • We report that by 10 wk of age hIAPP mice develop diabetes with a deficit in beta-cell mass due to increased beta-cell apoptosis. (mendeley.com)
  • The rIAPP transgenic mice counterparts do not develop diabetes or have decreased beta-cell mass. (mendeley.com)
  • The sequence comparison of these sites with the consensus binding site (), TT/GNNGNAAT/G, is shown in the inset. (nih.gov)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • The 5′-flanking sequence of the chicken FTO gene contains nine predicted binding sites for CCAAT/enhancer binding protein beta (C/EBP beta) and one for signal transducer and activator of transcription 3 (STAT3). (biomedcentral.com)
  • Additionally, sequence variation at C/EBP site I, which lies immediately upstream of the distal nuclear factor kappa B site and immediately downstream of a binding site for activating transcription factor (ATF)/cyclic AMP response element binding protein (CREB), has been shown to affect HIV-1 long terminal repeat (LTR) activity. (asm.org)
  • Most importantly, sequence variation at the ATF/CREB binding site affected basal LTR activity as well as LTR function following interleukin-6 stimulation, a treatment that leads to increases in C/EBP activation. (asm.org)
  • 19,20 Notably, 14-3-3 proteins were the first molecules identified as discrete binding partners of phosphoserine/threonine (pSer/Thr) residues in proteins, recognizing the sequence RSXpSXP or RXXXpSXP. (ahajournals.org)
  • The domain organization and sequence comparison of Mef2 proteins from representative species. (wikiversity.org)
  • The marker SNP in TRPM2 is a synonymous substitution and does not change the amino acid or protein sequence and is thus unlikely to be causally associated with disease. (biomedcentral.com)
  • Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and sequence-specific DNA binding . (genecards.org)
  • In this mature stage, miRNAs are loaded into a RNA-induced silencing complex (RISC) and bind predominantly to the 3'UTR of complementary target mRNAs using a 6-8 nucleotides stretch known as the seed sequence [ 3 ]. (hindawi.com)
  • Depending on sequence complementarity and most likely other as yet unknown mechanisms, this binding either obstructs the protein translational machinery and/or induces mRNA decay [ 4 ], indicating, therefore, the fate of the gene transcripts [ 5 ]. (hindawi.com)
  • One part of the domain contains a region that mediates sequence specific DNA binding properties and the leucine zipper that is required to hold together (dimerize) two DNA binding regions. (genenames.org)
  • [10] Interaction of SRF with other proteins, such as steroid hormone receptors, may contribute to regulation of muscle growth by steroids. (wikiversity.org)
  • Bai Y, Wei Y, Wu L, Wei J, Wang X, Bai Y. C/EBP beta mediates endoplasmic reticulum stress regulated inflammatory response and extracellular matrix degradation in LPS-stimulated human periodontal ligament cells. (springer.com)
  • How chromatin-binding modules interpret histone modifications: lessons from professional pocket pickers. (nature.com)
  • Recent observations have shown two CCAAT/enhancer binding protein (C/EBP) binding sites to be critically important for efficient human immunodeficiency virus type 1 (HIV-1) replication within cells of the monocyte/macrophage lineage, a cell type likely involved in transport of the virus to the brain. (asm.org)
  • Additional studies indicated that these two C/EBP binding sites were required for replication of an infectious HIV-1 molecular clone in the U-937 cell line as well as in primary cells of the monocyte/macrophage lineage. (asm.org)
  • First, low amounts of CREB-1 and C/EBP appear to heterodimerize and bind to a site consisting of a half site from both the ATF/CREB and C/EBP binding sites. (asm.org)
  • In addition, CREB-1 homodimers bind to the ATF/CREB site and recruit C/EBP dimers to their cognate weak binding sites. (asm.org)
  • This interaction is reciprocal, since C/EBP dimer binding to a strong C/EBP site leads to enhanced CREB-1 recruitment to ATF/CREB sites that are weakly bound by CREB. (asm.org)
  • The Ca 2+ /cAMP response element-binding protein (CREB) was initially identified as the main interlocutor in the dialogue between the synapse and the nucleus [1]. (wikiversity.org)
  • The antiapoptotic PPARδ might affect different proteins in endothelial cells, with 14-3-3 proteins being key candidates because of their ability to promote cell survival. (ahajournals.org)
  • Because 14-3-3 proteins and 14-3-3ε are antiapoptotic and antiinflammatory molecules in endothelial cells, they may play an important role in atherothrombosis. (ahajournals.org)
  • The C/EBP family of proteins includes at least eight different proteins, many of which are important activators of transcription. (asm.org)
  • Fu D, Lala-Tabbert N, Lee H, Wiper-Bergeron N. Mdm2 promotes myogenesis through the ubiquitination and degradation of CCAAT/enhancer-binding protein beta. (springer.com)
  • Finally, targeting stromal fibromuscular AR with the AR degradation enhancer, ASC-J9®, resulted in the reduction of PIN development/progression, which might provide a new approach to suppress PIN development. (wiley.com)
  • We previously demonstrated that the constitutive phosphorylation of ALK chimeric proteins is sufficient to induce cellular transformation in vitro and in vivo and that ALK activity is strictly required for the survival of ALK-positive ALCL cells. (nih.gov)
  • MacDonald M, Brown L, Longacre M, Stoker S, Kendrick M, Hasan N. Knockdown of both mitochondrial isocitrate dehydrogenase enzymes in pancreatic beta cells inhibits insulin secretion. (labome.org)
  • A role for CCAAT/enhancer binding protein beta-liver-enriched inhibitory protein in mammary epithelial cell proliferation. (mysciencework.com)
  • A negative regulator of beta-catenin signaling which is mutant in ADENOMATOUS POLYPOSIS COLI and GARDNER SYNDROME. (curehunter.com)
  • 12-14 Furthermore, recent evidence suggests that 14-3-3 proteins modulate crucial aspects of heart function both in vitro and in vivo. (ahajournals.org)
  • Recombinant Murine FGF-basic is a 16.3 kDa protein consisting of 145 amino acid residues. (peprotech.com)
  • The DNA binding region comprises a number of basic amino acids such as arginine and lysine. (genenames.org)
  • Leukemic HRX fusion proteins inhibit GADD34-induced apoptosis and associate with the GADD34 and hSNF5/INI1 proteins. (nih.gov)
  • The islet in type 2 diabetes is characterized by an approximately 60% beta-cell deficit, increased beta-cell apoptosis, and islet amyloid derived from islet amyloid polypeptide (IAPP). (mendeley.com)
  • We previously reported that overexpression of hIAPP in transgenic rats triggered endoplasmic reticulum (ER) stress-induced apoptosis in beta-cells. (mendeley.com)
  • All of these Fos family proteins, however, are highly unstable and return to basal levels within hours of drug administration. (royalsocietypublishing.org)
  • First, ΔFosB lacks two degron domains present in the C-terminus of full-length FosB (and found in all other Fos family proteins as well). (royalsocietypublishing.org)
  • The different C/EBP family members homo- and heterodimerize through their leucine zipper regions and bind to their cognate DNA sequences through the corresponding basic regions. (asm.org)