A CCAAT-enhancer-binding protein found in LIVER; ADIPOSE TISSUE; INTESTINES; LUNG; ADRENAL GLANDS; PLACENTA; OVARY and peripheral blood mononuclear cells (LEUKOCYTES, MONONUCLEAR). Experiments with knock-out mice have demonstrated that CCAAT-enhancer binding protein-alpha is essential for the functioning and differentiation of HEPATOCYTES and ADIPOCYTES.
A class of proteins that were originally identified by their ability to bind the DNA sequence CCAAT. The typical CCAAT-enhancer binding protein forms dimers and consists of an activation domain, a DNA-binding basic region, and a leucine-rich dimerization domain (LEUCINE ZIPPERS). CCAAT-BINDING FACTOR is structurally distinct type of CCAAT-enhancer binding protein consisting of a trimer of three different subunits.
Promoter Regions, Genetic
Enhancer Elements, Genetic
Gene Expression Regulation
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Gene Expression Regulation, Leukemic
Leukemia, Myeloid, Acute
Transcription Factor CHOP
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Electrophoretic Mobility Shift Assay
An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.
Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.
Transcription Factor AP-2
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Formation of MYELOID CELLS from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW via MYELOID STEM CELLS. Myelopoiesis generally refers to the production of leukocytes in blood, such as MONOCYTES and GRANULOCYTES. This process also produces precursor cells for MACROPHAGE and DENDRITIC CELLS found in the lymphoid tissue.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
GTP-Binding Protein alpha Subunits
The GTPase-containing subunits of heterotrimeric GTP-binding proteins. When dissociated from the heterotrimeric complex these subunits interact with a variety of second messenger systems. Hydrolysis of GTP by the inherent GTPase activity of the subunit causes it to revert to its inactive (heterotrimeric) form. The GTP-Binding protein alpha subunits are grouped into families according to the type of action they have on second messenger systems.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
Tumor Cells, Cultured
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Gene Expression Regulation, Enzymologic
DNA-binding motifs formed from two alpha-helixes which intertwine for about eight turns into a coiled coil and then bifurcate to form Y shaped structures. Leucines occurring in heptad repeats end up on the same sides of the helixes and are adjacent to each other in the stem of the Y (the "zipper" region). The DNA-binding residues are located in the bifurcated region of the Y.
Regulatory Sequences, Nucleic Acid
Hepatocyte Nuclear Factor 3-beta
Amino Acid Sequence
Reverse Transcriptase Polymerase Chain Reaction
NF-kappa B p50 Subunit
RNA Polymerase Sigma 54
Cyclic AMP Response Element-Binding Protein
Recombinant Fusion Proteins
Receptors, Cytoplasmic and Nuclear
Intracellular receptors that can be found in the cytoplasm or in the nucleus. They bind to extracellular signaling molecules that migrate through or are transported across the CELL MEMBRANE. Many members of this class of receptors occur in the cytoplasm and are transported to the CELL NUCLEUS upon ligand-binding where they signal via DNA-binding and transcription regulation. Also included in this category are receptors found on INTRACELLULAR MEMBRANES that act via mechanisms similar to CELL SURFACE RECEPTORS.
A group of CORTICOSTEROIDS that affect carbohydrate metabolism (GLUCONEOGENESIS, liver glycogen deposition, elevation of BLOOD SUGAR), inhibit ADRENOCORTICOTROPIC HORMONE secretion, and possess pronounced anti-inflammatory activity. They also play a role in fat and protein metabolism, maintenance of arterial blood pressure, alteration of the connective tissue response to injury, reduction in the number of circulating lymphocytes, and functioning of the central nervous system.
RNA, Small Interfering
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
Gene Expression Regulation, Neoplastic
Transcription Factor AP-1
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Endoplasmic Reticulum Stress
Monocyte-Macrophage Precursor Cells
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
A primary malignant neoplasm of epithelial liver cells. It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. The cells may be uniform or markedly pleomorphic, or form GIANT CELLS. Several classification schemes have been suggested.
Monosaccharide Transport Proteins
Gene Expression Profiling
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. This catalyzes endonucleolytic cleavage of DNA yielding 5'-phosphodi- and oligonucleotide end-products. The enzyme has a preference for double-stranded DNA.
Fatty Acid-Binding Proteins
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Oligonucleotide Array Sequence Analysis
Thapsigargin suppresses phorbol ester-dependent human involucrin promoter activity by suppressing CCAAT-enhancer-binding protein alpha (C/EBPalpha) DNA binding. (1/560)Human involucrin (hINV) is a keratinocyte differentiation marker expressed in the suprabasal epidermal layers. In cultured keratinocytes hINV mRNA levels are increased 10-fold by a 24-h treatment with 50 ng/ml PMA, an agent that promotes keratinocyte differentiation. Previous studies show that thapsigargin (TGN), an agent that depletes intracellular calcium stores, inhibits keratinocyte differentiation. In the present study we show that TGN inhibits the PMA-dependent, differentiation-associated, increase in hINV mRNA levels and hINV promoter activity. Inhibition is half-maximal at 10 nM and maximal at 100 nM TGN. Neither basal hINV promoter activity nor glyceraldehyde-3-phosphate dehydrogenase mRNA levels are inhibited. Mutation of a functionally important CAATT-enhancer-binding protein (C/EBP) site within the hINV promoter proximal regulatory region eliminates the regulation, suggesting that TGN may effect C/EBP-dependent promoter activation. Consistent with this hypothesis, TGN inhibits C/EBPalpha-dependent promoter activation via a mechanism that involves inhibition of C/EBPalpha binding to DNA without changing C/EBPalpha protein levels. These results suggest that TGN interferes with hINV expression by interfering with C/EBP transcription-factor function. (+info)
Identification and characterization of leptin-containing intracellular compartment in rat adipose cells. (2/560)The major leptin-containing membrane compartment was identified and characterized in rat adipose cells by means of equilibrium density and velocity sucrose gradient centrifugation. This compartment appears to be different from peptide-containing secretory granules present in neuronal, endocrine, and exocrine cells, as well as from insulin-sensitive GLUT-4-containing vesicles abundant in adipocytes. Exocytosis of both leptin- and GLUT-4-containing vesicles can be induced by insulin; however, only leptin secretion is responsive to serum stimulation. This latter effect is resistant to cycloheximide, suggesting that serum triggers the release of a stored pool of presynthesized leptin molecules. We conclude that regulated secretion of leptin and insulin-dependent translocation of GLUT-4 represent different pathways of membrane trafficking in rat adipose cells. NIH 3T3 cells ectopically expressing CAAT box enhancer binding protein-alpha and Swiss 3T3 cells expressing peroxisome proliferator-activated receptor-gamma undergo differentiation in vitro and acquire adipocyte morphology and insulin-responsive glucose uptake. Only the former cell line, however, is capable of leptin secretion. Thus different transcriptional mechanisms control the developmental onset of these two major and independent physiological functions in adipose cells. (+info)
Role of C/EBP homologous protein (CHOP-10) in the programmed activation of CCAAT/enhancer-binding protein-beta during adipogenesis. (3/560)Hormone induction of growth-arrested preadipocytes triggers mitotic clonal expansion followed by expression of CCAAT/enhancer-binding protein (C/EBP)alpha and differentiation into adipocytes. The order of these events is critical because C/EBPalpha is antimitotic and its expression prematurely would block the mitotic clonal expansion required for differentiation. C/EBPbeta, a transcriptional activator of the C/EBPalpha gene, is expressed early in the differentiation program, but lacks DNA-binding activity and fails to localize to centromeres until preadipocytes traverse the G(1)-S checkpoint of mitotic clonal expansion. Evidence is presented that dominant-negative CHOP-10 expressed by growth-arrested preadipocytes transiently sequesters C/EBPbeta by heterodimerization. As preadipocytes reach S phase, CHOP-10 is down-regulated, apparently releasing C/EBPbeta from inhibitory constraint and allowing transactivation of the C/EBPalpha gene. In support of these findings, up-regulation of CHOP-10 with the protease inhibitor N-acetyl-Leu-Leu-norleucinal prevents activation of C/EBPbeta, expression of C/EBPalpha, and adipogenesis. (+info)
Sequential repression and activation of the CCAAT enhancer-binding protein-alpha (C/EBPalpha ) gene during adipogenesis. (4/560)CCAAT enhancer-binding protein-alpha (C/EBPalpha) functions as a pleiotropic transcriptional activator of adipocyte genes during adipogenesis. Nuclear factor C/EBP undifferentiated protein (CUP), an isoform of activator protein-2alpha (AP-2alpha), binds to repressive elements in the C/EBPalpha gene promoter, silencing the gene until late in the differentiation program. The CUP regulatory element overlaps a Sp (GT-box) element in the promoter to which Sp3 (or Sp1) can bind. Binding by Sp3 or Sp1 and CUP/AP2-alpha is mutually exclusive. Sp3 is a strong transcriptional activator of the C/EBPalpha gene promoter in 3T3-L1 preadipocytes and Schneider cells, this activation being repressed by CUP/AP-2alpha. Sp3 is expressed throughout differentiation, whereas CUP/AP-2alpha, which is expressed only by preadipocytes, is down-regulated during differentiation coincident with transcription of the C/EBPalpha gene. Thus, CUP/AP-2alpha delays access of Sp3 to the Sp regulatory element, preventing premature expression of C/EBPalpha and thereby interference by C/EBPalpha (which is antimitotic) with mitotic clonal expansion, an essential early event in the differentiation program. (+info)
CREB (cAMP response element binding protein) and C/EBPalpha (CCAAT/enhancer binding protein) are required for the superstimulation of phosphoenolpyruvate carboxykinase gene transcription by adenoviral E1a and cAMP. (5/560)In the present study, we observed superstimulated levels of cAMP-stimulated transcription from the phosphoenolpyruvate carboxykinase (PEPCK) gene promoter in cells infected with wild-type adenovirus expressing 12 S and 13 S E1a proteins, or in cells expressing 13 S E1a alone. cAMP-stimulated transcription was inhibited in cells expressing only 12 S E1a, but slightly elevated in cells expressing E1a proteins with mutations in conserved regions 1 or 2, leading us to conclude that the superstimulation was mediated by conserved region 3 of 13 S E1a. E1a failed to enhance cAMP-stimulated transcription from promoters containing mutations that abolish binding by cAMP response element binding protein (CREB) or CCAAT/enhancer binding proteins (C/EBPs). This result was supported by experiments in which expression of dominant-negative CREB and/or C/EBP proteins repressed E1a- and cAMP-stimulated transcription from the PEPCK gene promoter. In reconstitution experiments using a Gal4-responsive promoter, E1a enhanced cAMP-stimulated transcription when chimaeric Gal4-CREB and Gal4-C/EBPalpha were co-expressed. Phosphorylation of CREB on serine-133 was stimulated in cells treated with dibutyryl cAMP, whereas phosphorylation of C/EBPalpha was increased by E1a expression. Our data support a model in which cAMP agonists increase CREB activity and stimulate PEPCK gene transcription, a process that is enhanced by E1a through the phosphorylation of C/EBPalpha. (+info)
The rat ortholog of the presumptive flounder antifreeze enhancer-binding protein is a helicase domain-containing protein. (6/560)The expression of winter flounder liver-type antifreeze protein (wflAFP) genes is tissue-specific and under seasonal and hormonal regulation. The only intron of the major wflAFP gene was demonstrated to be a liver-specific enhancer in both mammalian cell lines and flounder hepatocytes. Element B, the core enhancer sequence, was shown to interact specifically with a liver-enriched transcription factor, CCAAT/enhancer-binding protein alpha (C/EBPalpha), as well as a presumptive antifreeze enhancer-binding protein (AEP). In this study, the identity of the rat AEP ortholog was revealed via its DNA-protein interaction with element B. It is a helicase-domain-containing protein, 988 amino acids in length, and is homologous to mouse Smubp-2, hamster Rip-1 and human Smubp-2. The specific binding between element B and AEP was confirmed by South-Western analysis and gel retardation assays. Residues in element B important to this interaction were identified by methylation interference assays. Mutation on one of the residues disrupted the binding between element B and AEP and its enhancer activity was significantly reduced, suggesting that AEP is essential for the transactivation of the wflAFP gene intron. The rat AEP is ubiquitously expressed in various tissues, and the flounder homolog is present as shown by genomic Southern analysis. The potential role of AEP in regulating the flounder AFP gene expression is discussed. (+info)
Interferon-gamma-induced regulation of peroxisome proliferator-activated receptor gamma and STATs in adipocytes. (7/560)Interferon-gamma (IFN-gamma) is known primarily for its roles in immunological responses but also has been shown to affect fat metabolism and adipocyte gene expression. To further investigate the effects of IFN-gamma on fat cells, we examined the effects of this cytokine on the expression of adipocyte transcription factors in 3T3-L1 adipocytes. Although IFN-gamma regulated the expression of several adipocyte transcription factors, IFN-gamma treatment resulted in a rapid reduction of both peroxisome proliferator-activated receptor (PPAR) protein and mRNA. A 48-h exposure to IFN-gamma also resulted in a decrease of both CCAAT/enhancer-binding alpha and sterol regulatory element binding protein (SREBP-1) expression. The short half-life of both the PPARgamma mRNA and protein likely contributed to the rapid decline of both cytosolic and nuclear PPARgamma in the presence of IFN-gamma. Our studies clearly demonstrated that the IFN-gamma-induced loss of PPARgamma protein is partially inhibited in the presence of two distinct proteasome inhibitors. Moreover, IFN-gamma also inhibited the transcription of PPARgamma, which was accompanied by a decrease in PPARgamma mRNA accumulation. In addition, exposure to IFN-gamma resulted in a substantial increase in STAT 1 expression and a small increase in STAT 3 expression. IFN-gamma treatment of 3T3-L1 adipocytes (48-96 h) resulted in a substantial inhibition of insulin-sensitive glucose uptake. These data clearly demonstrate that IFN-gamma treatment results in the development of insulin resistance, which is accompanied by the regulation of various adipocyte transcription factors, in particular the synthesis and degradation of PPARgamma. (+info)
Hormonal signaling and transcriptional control of adipocyte differentiation. (8/560)Recent advances regarding the biology of adipose tissue have identified the adipocyte as an important mediator in many physiologic and pathologic processes regarding energy metabolism. Consideration for a central role of adipose tissue in the development of obesity, cardiovascular disease and noninsulin-dependent diabetes mellitus has resulted in new incentives toward understanding the complexities of adipocyte differentiation. Current knowledge of this process includes a cascade of transcriptional events that culminate in the expression of peroxisome proliferator-activated receptor-gamma (PPARgamma) and CCAAT/enhancer binding protein-alpha (C/EBPalpha). These prominent adipogenic transcription factors have been shown to regulate, directly or indirectly, the gene expression necessary for the development of the mature adipocyte. Hormonal and nutritional signaling that impinges on these trans-acting factors provides a molecular link between lipids and lipid-related compounds and the gene expression important for glucose and lipid homeostasis. Knowledge concerning the transcriptional events mediating adipocyte differentiation provides a basis for understanding the physiologic processes associated with adipose tissue as well as for the development of therapeutic interventions in obesity and its related disorders. (+info)
CCAAT-enhancer binding protein (C/EBP) β regulates insulin-like growth factor (IGF) 1 expression in porcine liver during...
CCAAT-enhancer binding protein (C/EBP) β regulates insulin-like growth factor (IGF) 1 expression in porcine liver during prenatal and postnatal ...
Animal Pharm: Sarcopenia: Poverty of the Flesh (Greek)
Adipocyte differentiation is a developmental process that is critical for metabolic homeostasis and nutrient signaling. The mammalian target of rapamycin (mTOR) mediates nutrient signaling to regulate cell growth, proliferation, and diverse cellular differentiation. It has been reported that rapamycin, the inhibitor of mTOR and an immunosuppressant, blocks adipocyte differentiation, but the mechanism underlying this phenomenon remains unknown. Here we show that mTOR plays a critical role in 3T3-L1 preadipocyte differentiation and that mTOR kinase activity is required for this process. Rapamycin specifically disrupted the positive transcriptional feedback loop between CCAAT/enhancer-binding protein-alpha and peroxisome proliferator-activated receptor-gamma (PPAR-gamma), two key transcription factors in adipogenesis, by directly targeting the transactivation activity of PPAR-gamma. In addition, we demonstrate for the first time that PPAR-gamma activity is dependent on amino acid sufficiency, ...
Tumor Necrosis Factora - Insulin Resistance - AmeriCorps Health Blog
TNF-a was originally identified as a macrophage product implicated in the metabolic disturbances of chronic inflammation and malignancy. Later on, its biological actions were shown to further extend to anorexia, weight loss, and insulin resistance (7). Elevated adipose tissue expression of TNF-a mRNA has been reported in different rodent models of obesity as well as in clinical studies involving obese patients (23). TNF-a mRNA expression is positively correlated with body adiposity as well as with hyperinsulinemia, showing positive associations with fasting insulin and triglyceride concentrations. TNF-a inhibits the expression of the transcription factor CCAAT/ enhancer binding protein-a (CEBPa) and the nuclear receptor peroxisome proliferator-activated receptor (PPAR)y2 (8,12,14). Furthermore, TNF-a stimulates the nuclear factor- kB transcription factor (NFkB), which orchestrates a series of inflammatory events, including expression of adhesion molecules on the surface of both endothelial cells ...
Active Motif » TransAM C/EBP (C/EBPa and C/EBPb, CCAAT-enhancer binding protein DNA binding ELISA)
TransAM C/EBP α/β Kits are DNA binding ELISAs that quantify the activated transcription factors using a method that is faster and more sensitive than gelshift, without radioactivity and gels.
CEBPA - Вікіпедія
Choi B.H., Park G.T., Rho H.M. (1999). Interaction of hepatitis B viral X protein and CCAAT/enhancer-binding protein alpha synergistically activates the hepatitis B viral enhancer II/pregenomic promoter.. J. Biol. Chem. 274: 2858 - 2865. PubMed DOI:10.1074/jbc.274.5.2858 ...
gq g11 gtp binding protein alpha subunits
Genomes and Genes, Scientific Experts, Publications, Species, Research Topics, Research Grants about gq g11 gtp binding protein alpha subunits
RCSB PDB - Protein Feature View - Purine-rich binding protein-alpha, isoform E - Q95RR6 (Q95RR6 DROME)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Upstream open reading frames: molecular switches in (patho)physiology | MDC Berlin
Conserved upstream open reading frames (uORFs) are found within many eukaryotic transcripts and are known to regulate protein translation. Evidence from genetic and bioinformatic studies implicates disturbed uORF-mediated translational control in the etiology of human diseases. A genetic mouse model has recently provided proof-of-principle support for the physiological relevance of uORF-mediated translational control in mammals. The targeted disruption of the uORF initiation codon within the transcription factor CCAAT/enhancer binding protein beta (C/EBPbeta) gene resulted in deregulated C/EBPbeta protein isoform expression, associated with defective liver regeneration and impaired osteoclast differentiation. The high prevalence of uORFs in the human transcriptome suggests that intensified search for mutations within 5 RNA leader regions may reveal a multitude of alterations affecting uORFs, causing pathogenic deregulation of protein expression.. ...
Stress responses are critical for estrogen (E2) to induce apoptosis in E2-deprived breast cancer cells. Nuclear factor-kappa B (NF-κB) is well known as a therapeutic target to prevent stress responses in chronic inflammatory diseases including cancer. However, whether E2 activates NF-κB to participate in stress-associated apoptosis in E2-deprived breast cancer cells is unclear. We demonstrated that E2 differentially modulates NF-κB activity in E2-deprived breast cancer cells according to the treatment time. Because E2 initially has significant potential to down modulate the NF-κB activation, it completely suppresses the tumor necrosis factor alpha (TNFα)-induced NF-κB activation. We found that E2 preferentially and constantly enhances the expression of transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) which is responsible for suppression of NF-κB activation by E2 in MCF-7:5C cells. The mTOR signaling pathway promotes repression of NF-κB by C/EBPβ which is confirmed by ...
Neutrophil-Specific Granule Deficiency disease: Malacards - Research Articles, Drugs, Genes, Clinical Trials
Neutrophil-specific granule deficiency (SGD) is a rare disorder characterized by recurrent pyogenic infections, defective neutrophil chemotaxis and bactericidal activity, and lack of neutrophil secondary granule proteins. It has been linked to a defect in the transcription factor CCAAT/enhancer binding protein (CEBP) epsilon. Recently, loss-of-function mutations in SMARCD2 were identified from SGD patients. SMARCD2 is chromatin-remodeling factor, that interacts with CEBP epsilon ...
Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy<...
TY - JOUR. T1 - Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy. AU - Sun, Yuansheng. AU - Day, Richard. AU - Periasamy, Ammasi. PY - 2011/9. Y1 - 2011/9. N2 - Fluorescence lifetime imaging microscopy (FLIM) is now routinely used for dynamic measurements of signaling events inside living cells, including detection of protein-protein interactions. An understanding of the basic physics of fluorescence lifetime measurements is required to use this technique. In this protocol, we describe both the time-correlated single photon counting and the frequency-domain methods for FLIM data acquisition and analysis. We describe calibration of both FLIM systems, and demonstrate how they are used to measure the quenched donor fluorescence lifetime that results from FÃ ¶rster resonance energy transfer (FRET). We then show how the FLIM-FRET methods are used to detect the dimerization of the transcription factor CCAAT/enhancer binding protein-Î ± in ...
FLIM Publications | W.M. Keck Center for Cellular Imaging, U.Va.
ABSTRACT: Fluorescence lifetime imaging microscopy (FLIM) is now routinely used for dynamic measurements of signaling events inside living cells, including detection of protein-protein interactions. An understanding of the basic physics of fluorescence lifetime measurements is required to use this technique. In this protocol, we describe both the time-correlated single photon counting and the frequency-domain methods for FLIM data acquisition and analysis. We describe calibration of both FLIM systems, and demonstrate how they are used to measure the quenched donor fluorescence lifetime that results from F?rster resonance energy transfer. We then show how the FLIM-FRET methods are used to detect the dimerization of the transcription factor CCAAT enhancer binding protein-a in live mouse pituitary cell nuclei. Notably, the factors required for accurate determination and reproducibility of lifetime measurements are described. With either method, the entire protocol including specimen preparation, ...
Differentiation-induced gene expression in 3T3-L1 preadipocytes: CCAAT/enhancer binding protein interacts with and activates...
TY - JOUR. T1 - Differentiation-induced gene expression in 3T3-L1 preadipocytes. T2 - CCAAT/enhancer binding protein interacts with and activates the promoters of two adipocyte-specific genes.. AU - Christy, R. J.. AU - Yang, V. W.. AU - Ntambi, J. M.. AU - Geiman, D. E.. AU - Landschulz, W. H.. AU - Friedman, A. D.. AU - Nakabeppu, Y.. AU - Kelly, T. J.. AU - Lane, M. D.. PY - 1989/9. Y1 - 1989/9. N2 - Previous studies have shown that differentiation of 3T3-L1 preadipocytes leads to the transcriptional activation of a group of adipose-specific genes. As an approach to defining the mechanism responsible for activating the expression of these genes, we investigated the binding of nuclear factors to the promoters of two differentiation-induced genes, the 422(aP2) and stearoyl-CoA desaturase 1 (SCD1) genes. DNase I footprinting and gel retardation analysis identified two binding regions within the promoters of each gene that interact with nuclear factors present in differentiated 3T3-L1 adipocytes. ...
CCAAT/enhancer binding protein zeta (mouse, aa620-633) Antibody (internal region) - Peptide-affinity purified goat antibody WB,...
CCAAT/enhancer binding protein zeta (mouse, aa620-633) Antibody (internal region), Peptide-affinity purified goat antibody validated in WB, E (AF3888a), Abgent
CCAAT/enhancer binding protein β directly regulates the expression of the complement component 3 gene in neural cells:...
In this work, we have shown that the transcription factor C/EBPβ directly regulates the expression of the C3 gene, and that this control could be relevant for the pro-inflammatory effects of this transcription factor. By microarray analysis and RT-PCR we showed that the hippocampal content of C3 transcripts was depleted in C/EBPβ −/− mice. The analysis of the C3 promoter showed that this gene was directly induced by C/EBPβ through a C/EBPβ consensus site located at −616/-599 position from the transcription start site. In accordance with these data, LPS induced the expression of C3 in glial cells, at least in part, through the induction of C/EBPβ since the repression of LPS-induction of C/EBPβ by shRNA interference blocked C3 increase. On the contrary, C/EBPβ overexpression by transient transfection induced C3 expression. Additionally, treatment of these cultures with LPS induced the levels of the pro-inflammatory factors IL-1β and COX-2, which were significantly reduced in those ...
CEBPD (C/EBP delta) is a member of the CCAAT-enhancer binding protein (C/EBP) family of transcription factors characterized by a b-Zip domain that mediates dimerization and DNA binding. CEBPD is induced in response to acute stressors such as cytokine stimulation, bacterial lipopolysaccharide (LPS), corticosteroids, radiation and hypoxia. We have previously reported that CEBPD has dual functions in breast cancer by both attenuating or enhancing oncogenic pathways depending on context (Balamurugan and Sterneck, 2013, Mendoza-Villanueva et al., 2016). Recent studies reveal that elevated Endoplasmic Reticulum (ER) stress is associated with the pathology of several diseases including cancer. Limiting supply of nutrients and oxygen in growing tumor cells disrupts the protein folding homeostasis resulting in activation of the unfolded protein response (UPR). The UPR includes pathways that support adaptation to stress, and that are also implicated in promoting malignant features and therapy resistance ...
FUNCTIONAL COOPERATION OF CEBPA AND TET2 MUTATIONS IN ACUTE... : HemaSphere
The transcription factor CCAAT-enhancer-binding protein alpha (C/EBPα) is a master regulator of granulopoiesis and regulates the switch between proliferating, uncommitted progenitors and cell-cycle-arrested, differentiated myeloid cells. Usage of two alternative translation initiation sites in the CEBPA mRNA results in expression of a full-length C/EBPα protein p42 (42 kDa) and a shorter p30 isoform (30 kDa). CEBPA mutations are found in 9-15% of Acute Myeloid Leukemia (AML) patients. N-terminal frameshift mutations in the CEBPA gene lead to selective ablation of p42 expression, while C-terminal mutations disrupt the dimerization and DNA-binding ability of C/EBPα. AML patients harbor either mono- or biallelic CEBPA mutations (CEBPAmo or CEBPAbi) and both genotypes are frequently associated with concurrent mutations in other genes. The most commonly co-occurring mutations in both groups are loss-of-function mutations in the methylcytosine dioxygenase TET2 (44.4% in CEBPAmo / 34.8% in CEBPAbi). ...
Browsing HMS Scholarly Articles by Author Raam, Manu S.
The CCAAT/Enhancer Binding Proteins (C/EBPs) are a family of leucine-zipper transcription factors that regulate physiological processes such as energy metabolism, inflammation, cell cycle, and the development and differentiation ...
Search of: MLLT3 - List Results - ClinicalTrials.gov
Pharmacodynamic studies, including micro-ribonucleic acid (miRNA)-181 family and target gene expression, CCAAT/enhancer binding protein (C/EBP), alpha gene (CEBPA) expression, and genes involved in erythroid ...
CEBPA - Overview: CEBPA Mutations, Gene Sequencing, Varies
CEBPA : Initial evaluation of acute myeloid leukemia, both for assigning an appropriate diagnostic subclassification and as an aid for determining prognosis
Identification of transcriptional activation and repression domains in human CCAAT/enhancer-binding protein ε<...
TY - JOUR. T1 - Identification of transcriptional activation and repression domains in human CCAAT/enhancer-binding protein ε. AU - Williamson, Elizabeth A.. AU - Xu, Haixin N.. AU - Gombart, Adrian F.. AU - Verbeek, Walter. AU - Chumakov, Alexey M.. AU - Friedman, Alan D.. AU - Koeffler, H. Phillip. PY - 1998/6/12. Y1 - 1998/6/12. N2 - Human CCAAT/enhancer-binding protein ε (C/EBPε), a new member of the C/EBP family, significantly upregulates both the mim-1 and human myeloperoxidase promoters, suggesting an important role for C/EBPε in the transcriptional regulation of a subset of myeloid-specific genes. To elucidate the structure and function of C/EBPε in transcriptional activation, amino acid residues 1-115, 147-249, or 1-249 of C/EBPε were fused to the yeast GAL4 DNA binding domain. These expression vectors were cotransfected with a chloramphenicol acetyltransferase reporter gene and, in all cell lines tested, only the GAL-C/EBPε-(1-115) fusion protein significantly activated ...
Human CEBPb(CCAAT/Enhancer Binding Protein Beta) ELISA Kit - Bio EMM
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human CCAAT/Enhancer Binding Protein Beta (CEBPb) in samples from Tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species ...
The PRKCH gene and its putative association with human ageing
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription ...
CCAAT/enhancer binding protein β directly regulates the expression of the complement component 3 gene in neural cells:...
The CCAAT/enhancer-binding protein β (C/EBPβ) is a transcription factor, which was first identified as a regulator of differentiation and inflammatory processes mainly in adipose tissue and liver; however, its function in the brain was largely unknown for many years. Previous studies from our laboratory indicated that C/EBPβ is implicated in inflammatory process and brain injury, since mice lacking this gene were less susceptible to kainic acid-induced injury. We first performed cDNA microarrays analysis using hippocampal RNA isolated from C/EBPβ +/+ and C/EBPβ −/− mice. Immunocytochemical and immunohistochemical studies were done to evaluate C/EBPβ and C3 levels. Transient transfection experiments were made to analyze transcriptional regulation of C3 by C/EBPβ. To knockdown C/EBPβ and C3 expression, mouse astrocytes were infected with lentiviral particles expressing an shRNA specific for C/EBPβ or an siRNA specific for C3. Among the genes displaying
CEBPZOS (CCAAT/enhancer binding protein (C/EBP), zeta, opposite strand) - KOMP (Knockout Mouse Project)
The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
The gene encoding an important myeloid transcription factor is mutated in cells of acute myeloid leukemia (somatic mutation) and in patients with autosomal dominant familial acute myeloid leukemia (germline mutation).. ...
INT251745 - wiki-pain
Having analysed data with TRANSFAC system, we may assume that the disturbed attachment of such factors as (C/EBP(CCAAT enhancer binding protein) Hoxa-3,Sp1 (serine protease inhibitor) or GATA-1, (GATA nucleotide sequence) may have an impact on IGF-1 protein synthesis, but we did not observed any significant correlation between promoter P1 polymorphism and serum IGF-1 levels ...
C4BPA Gene - GeneCards | C4BPA Protein | C4BPA Antibody
Complete information for C4BPA gene (Protein Coding), Complement Component 4 Binding Protein Alpha, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
PLOS ONE: Role of Macrophage CCAAT/Enhancer Binding Protein Delta in the Pathogenesis of Rheumatoid Arthritis in Collagen...
Background The up-regulation of CCAAT/enhancer binding protein delta (CEBPD) has frequently been observed in macrophages in age-associated disorders, including rheumatoid arthritis (RA). However, the role of macrophage CEBPD in the pathogenesis of RA is unclear. Methodology and Principal Findings We found that the collagen-induced arthritis (CIA) score and the number of affected paws in Cebpd−/− mice were significantly decreased compared with the wild-type (WT) mice. The histological analysis revealed an attenuated CIA in Cebpd−/− mice, as shown by reduced pannus formation and greater integrity of joint architecture in affected paws of Cebpd−/− mice compared with WT mice. In addition, immunohistochemistry analysis revealed decreased pannus proliferation and angiogenesis in Cebpd−/− mice compared with WT mice. CEBPD activated in macrophages played a functional role in promoting the tube formation of endothelial cells and the migration and proliferation of synoviocytes. In vivo DNA
CEBPA mutations in patients with de novo acute myeloid leukemia: data | OTT
CEBPA mutations in patients with de novo acute myeloid leukemia: data analysis in a Chinese population Long Su, SuJun Gao, XiaoLiang Liu, YeHui Tan, Lu Wang, Wei Li Cancer Center, The First Hospital, Jilin University, Changchun, Peoples Republic of China Background: This study was aimed to explore the clinical characteristics and prognoses of acute myeloid leukemia (AML) patients with CEBPA mutations. Patients and methods: Three hundred and forty-five patients with de novo AML were retrospectively analyzed with regard to CEBPA mutations, clinical characteristics, therapeutic responses, and long-term outcomes. Results: CEBPA mutations were detected in 59 patients (17.10%), with 47 cases harboring double mutations and 12 cases harboring single mutations. In those with a normal karyotype (NK), 44 cases (25.29%) were detected with CEBPA mutations. The following characteristics were observed in CEBPA-mutated patients: most (66.10%) of them were M1 or M2; they presented with higher peripheral white blood
CCAAT/enhancer-binding protein δ (CEBPD) is expressed | Open-i
CCAAT/enhancer-binding protein δ (CEBPD) is expressed in hypoxic kidney tubular cells in vivo. (a) Mice were exposed to 8% O2 for 6 h using a hypoxia chamber
Peroxisome Proliferator-Activated Receptor-δ Upregulates 14-3-3ε in Human Endothelial Cells via CCAAT/Enhancer Binding Protein...
Here, we investigated the mechanisms by which PPARδ agonists control expression of 14-3-3ε, a key antiinflammatory protein in endothelial cells.12 Our data not only provide evidence that PPARδ modulates expression of YWHAE gene and 14-3-3ε protein under resting conditions but also demonstrate that this nuclear receptor upregulates 14-3-3ε expression by targeting transcription via a PPRE-independent pathway involving colocalization of C/EBPβ and PPARδ on YWHAE promoter. Several lines of evidence support these conclusions. First, PPARδ agonists regulated YWHAE promoter activity in a concentration- and time-dependent manner. Concordantly, YWHAE promoter was upregulated by PPARδ overexpression, whereas specific PPARγ and PPARα ligands had no effect on YWHAE promoter under our experimental conditions. Second, PPARδ activation increased 14-3-3ε mRNA and protein expression in both primary and spontaneously transformed endothelial cell lines, whereas PPARδ knockdown depressed basal and ...
CCAAT/Enhancer Binding Protein and Nuclear Factor-Y Regulate Adiponectin Gene Expression in Adipose Tissue | Diabetes
FIG. 4. Effect of site-specific mutations on C/EBP and NF-Y binding activity. Nuclear extracts were prepared from 293T cells overexpressing C/EBP-α or -β. For mock, the cells were transfected by pcDNA. All probes were generated by PCR with 32P-labeled antisense primer and unlabeled sense primers using site-directed mutated plasmids as templates, and purified from the polyacrylamide gel, to equalize the specific activities between probes. A: DNA oligonucleotide sequence of wild and mutated probes used in EMSA. Mutated regions are indicated by lowercase with * with their name. B: EMSA using nuclear extracts of 293T cells overexpression C/EBP-α and C/EBP-β. Wild-type (wt) and mutant (m1, m2, m3, m4, and m5) probes were incubated with 4 μg of indicated nuclear extracts. The bands corresponding to NF-Y, C/EBP-α, and C/EBP-β are marked by arrows. C: Western blot (WB) analysis of C/EBP-α and -β for validating the expression of C/EBP-α and -β. 293T cells were transfected with expression ...
Regulation of Id2 expression by CCAAT/enhancer binding protein beta. - PubMed - NCBI
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
C/EBPα and DEK coordinately regulate myeloid differentiation
The transcription factor C/EBPα is a critical mediator of myeloid differentiation and is often functionally impaired in acute myeloid leukemia. Recent studies have suggested that oncogenic FLT3 activity disrupts wild-type C/EBPα function via phosphorylation on serine 21 (S21). Despite the apparent r …
Overexpression of DR-nm23, a protein encoded by a member of the nm23 gene family, inhibits granulocyte differentiation and...
Sigma-Aldrich offers abstracts and full-text articles by [D Venturelli, R Martinez, P Melotti, I Casella, C Peschle, C Cucco, G Spampinato, Z Darzynkiewicz, B Calabretta].
Targeting the C/EBPalpha-Gfi-pathway in CML stem cells - Bruno Calabretta
The transcription factor C/EBP? is required for regulation of the balance between differentiation and proliferation during the early stages of myelopoiesis. The...
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Vascular Inflammation Is Negatively Autoregulated by Interaction Between CCAAT/Enhancer-Binding Protein-δ and Peroxisome...
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Molecular mechanisms of C/EBP alpha mediated growth arrest - Nikolai Timchenko
Liver is a unique tissue which is able to regenerate in response to partial hepatectomy (PH) and after injury. My laboratory investigates the role of transcript...
BCL2 | Cancer Genetics Web
Methamphetamine (METH) is a widely abused illicit psychoactive drug. Our previous study has shown that CCAAT-enhancer binding protein β (C/EBPβ) is an important regulator in METH-induced neuronal autophagy and apoptosis. However, the detailed molecular mechanisms underlying this process remain poorly understood. Previous studies have demonstrated that DNA damage-inducible transcript 4 (DDIT4), Trib3 (tribbles pseudo kinase 3), alpha-synuclein (α-syn) are involved in METH-induced dopaminergic neurotoxicity. We hypothesized that C/EBPβ is involved in METH-induced DDIT4-mediated neuronal autophagy and Trib3-mediated neuronal apoptosis. We tested our hypothesis by examining the effects of silencing C/EBPβ, DDIT4, Trib3 or α-syn with small interfering ribonucleic acid (siRNA) on METH-induced autophagy and apoptosis in the human neuroblastoma SH-SY5Y cells. We also measured the levels of phosphorylated tuberous sclerosis complex 2 (TSC2) protein and Parkin protein level in SH-SY5Y cells. ...
Neurotrophin/Trk receptor signaling mediates C/EBPalpha, -beta and NeuroD recruitment to immediate-early gene promoters in...
BACKGROUND: Extracellular signaling through receptors for neurotrophins mediates diverse neuronal functions, including survival, migration and differentiation in the central nervous system, but the transcriptional targets and regulators that mediate these diverse neurotrophin functions are not well understood. RESULTS: We have identified the immediate-early (IE) genes Fos, Egr1 and Egr2 as transcriptional targets of brain derived neurotrophic factor (BDNF)/TrkB signaling in primary cortical neurons, and show that the Fos serum response element area responds to BDNF/TrkB in a manner dependent on a combined C/EBP-Ebox element. The Egr1 and Egr2 promoters contain homologous regulatory elements. We found that C/EBPalpha/beta and NeuroD formed complexes in vitro and in vivo, and were recruited to all three homologous promoter regions. C/EBPalpha and NeuroD co-operatively activated the Fos promoter in transfection assays. Genetic depletion of Trk receptors led to impaired recruitment of C/EBPs and NeuroD in
CEBPE - Wikipedia
CCAAT/enhancer binding protein (C/EBP), epsilon, also known as CEBPE and CRP1, is a type of ccaat-enhancer-binding protein. CEBPE is its human gene and is pro-apoptotic. The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-δ. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with specific granule deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined. GRCh38: Ensembl release 89: ENSG00000092067 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000052435 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Entrez Gene: CEBPE CCAAT/enhancer binding protein (C/EBP), epsilon. Antonson P, Stellan B, Yamanaka R, ...
Part 05: CHROMOSOMES
A recent paper in Nature Medicine showed that Down syndrome brains have reduced expression of Sorting nexin 27 (SNX27) and CCAAT/enhancer binding protein beta (C/EBP beta) and identified C/EBP beta as a transcription factor for SNX27. Down syndrome results in overexpression of miR-155, a chromosome 21-encoded microRNA that negatively regulates C/EBP beta, thereby reducing SNX27 expression. SNX27 is a brain-enriched […]. ...
Regenerating livers of old rats contain high levels of C/EBPα that correlate with altered expression of cell cycle associated...
TY - JOUR. T1 - Regenerating livers of old rats contain high levels of C/EBPα that correlate with altered expression of cell cycle associated proteins. AU - Timchenko, Nikolai A.. AU - Wilde, Margaret. AU - Kosai, Ken Lchiro. AU - Heydari, Ahmed. AU - Bilyeu, Timothy A.. AU - Finegold, Milton J.. AU - Mohamedali, Khalid. AU - Richardson, Arlan. AU - Darlington, Gretchen J.. N1 - Funding Information: We thank W.Harper, S.Elledge and E.Harlow for cp36 antibodies and Dr J.Albrecht for His-C-p21. We thank K.Faraj for excellent assistance in the preparation of the manuscript. This work was supported by NIH grants DK45285 (G.J.D.), AG13663 (G.J.D.), AG00765-01 (N.A.T.) and GM55188-01 (N.A.T.), by AFAR grant A 97161, by The Moran Foundation and by the Estate of Evelyn Lucille Hansen.. PY - 1998/7/1. Y1 - 1998/7/1. N2 - The nuclear transcription factor, CCAAT/enhancer binding protein α (C/EBPα) is expressed at high levels in the liver and inhibits growth in cultured cells. We have tested the ...
DNMT1-interacting RNAs block gene-specific DNA methylation
DNA methylation was first described almost a century ago; however, the rules governing its establishment and maintenance remain elusive. Here we present data demonstrating that active transcription regulates levels of genomic methylation. We identify a novel RNA arising from the CEBPA gene locus tha …
Karen D. Parfitt | Pomona College in Claremont, California - Pomona College
Morrissey, J.M., Bigus, E. 19#, Necarsulmer, J.C., 15#, Srinivasan, V. 16# , Peppercorn, K., Mockett, B.G., Tate, W.P., Hughes, S.M., Parfitt, K.D. and Abraham, W.C. (2019) The Tripeptide RER Mimics Secreted Amyloid Precursor Protein-Alpha in Upregulating LTP. Frontiers in Cellular Neuroscience, 13:459-471. (#: undergraduate, Pomona College).. Mockett, B.G., Guévremont, D., Elder, M., Parfitt, K.D., Peppercorn, K., Morrissey, J., Singh, A., Hintz,T.J., Kochen, L., Tom Dieck, S, Schuman, E.M, Tate, W.P., Williams, J.M., and Abraham, W.C. (2019) Glutamate receptor trafficking and protein synthesis mediate the facilitation of LTP by secreted amyloid precursor protein-alpha; Journal of Neuroscience, 39: 3188-3203. Wiertelak, E.P., Hardwick, J., Kerchner, M., Parfitt, K.D., & Ramirez, J.J. The New Blueprints: Undergraduate Neuroscience Education in the Twenty-First Century, (2018) Journal of Undergraduate Neuroscience Education. Tan, V.T.Y., Mockett, B.G., Ohline, S., Parfitt, K.D., Wickie, ...
KAKEN - Research Projects | Gene Expression of Myeloperoxidase during Granulocyte Differentiation (KAKENHI-PROJECT-05680549)
Principal Investigator：YAMADA Michiyuki, Project Period (FY)：1993 - 1994, Research Category：Grant-in-Aid for General Scientific Research (C), Research Field：Functional biochemistry
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March 2001). "Dominant-negative mutations of CEBPA, encoding CCAAT/enhancer binding protein-alpha (C/EBPalpha), in acute ... an aggregate of multiple copies of the same protein, otherwise known as a homomultimeric protein or homooligomeric protein. In ... For example, in humans the Hb gene locus is responsible for the Beta-chain protein (HBB) that is one of the two globin proteins ... In fact, the first study reporting a mutant protein inhibiting the normal function of a wild-type protein in a mixed multimer ...
2003). "Transcriptional regulation of human CYP3A4 basal expression by CCAAT enhancer-binding protein alpha and hepatocyte ... sequence-specific DNA binding. • DNA binding. • transcription factor binding. • protein domain specific binding. • RNA ... HNF-3G is a member of the forkheadclass of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional ... transcription factor activity, sequence-specific DNA binding. • transcription regulatory region DNA binding. • ...
CEBPA - Вікіпедія
CEBPA, C/EBP-alpha, CEBP, CCAAT/enhancer binding protein alpha, CCAAT enhancer binding protein alpha. ... Choi B.H., Park G.T., Rho H.M. (1999). Interaction of hepatitis B viral X protein and CCAAT/enhancer-binding protein alpha ... and expression patterns of the human gene encoding CCAAT/enhancer binding protein alpha (C/EBP alpha).. Biochem. Biophys. Res. ... CEBPA (англ. CCAAT/enhancer binding protein alpha) - білок, який кодується однойменним геном, розташованим у людей на короткому ...
"Tumor Necrosis Factor Alpha Inhibits Type I Collagen Synthesis through Repressive CCAAT/Enhancer-Binding Proteins". Mol. Cell. ... CCAAT/enhancer-binding protein beta is a protein that in humans is encoded by the CEBPB gene. The protein encoded by this ... "Entrez Gene: CEBPB CCAAT/enhancer binding protein (C/EBP), beta". Ruffell D, Mourkioti F, Gambardella A, Kirstetter P, Lopez RG ... Chen GK, Sale S, Tan T, Ermoian RP, Sikic BI (April 2004). "CCAAT/enhancer-binding protein beta (nuclear factor for interleukin ...
CCAAT/enhancer-binding protein delta is a protein that in humans is encoded by the CEBPD gene. The protein encoded by this ... It can also form heterodimers with the related protein CEBP-alpha. The encoded protein is important in the regulation of genes ... "CCAAT/enhancer-binding protein family members recruit the coactivator CREB-binding protein and trigger its phosphorylation". ... "Entrez Gene: CEBPD CCAAT/enhancer binding protein (C/EBP), delta". Gery S, Tanosaki S, Hofmann WK, Koppel A, Koeffler HP (Feb ...
March 2001). "Dominant-negative mutations of CEBPA, encoding CCAAT/enhancer binding protein-alpha (C/EBPalpha), in acute ... an aggregate of multiple copies of the same protein, otherwise known as a homomultimeric protein or homooligomeric protein. In ... For example, in humans the Hb gene locus is responsible for the Beta-chain protein (HBB) that is one of the two globin proteins ... A mutation that leads to a mutant protein that disrupts the activity of the wild-type protein in the multimer is a dominant- ...
"Transcriptional regulation of human CYP3A4 basal expression by CCAAT enhancer-binding protein alpha and hepatocyte nuclear ... "Cloning and characterization of seven human forkhead proteins: binding site specificity and DNA bending". The EMBO Journal. 13 ... Forkhead box I1 is a protein that in humans is encoded by the FOXI1 gene. This gene belongs to the forkhead family of ... FOX proteins GRCh38: Ensembl release 89: ENSG00000168269 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000047861 - ...
Transcription elongation regulator 1
... of a co-repressor that inhibits the transcriptional and growth-arrest activities of CCAAT/enhancer-binding protein alpha". J. ... 2000). "Protein-interaction modules that organize nuclear function: FF domains of CA150 bind the phosphoCTD of RNA polymerase ... 2004). "A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease". Mol. Cell. ... This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein ...
Jurado LA, Song S, Roesler WJ, Park EA (2002). "Conserved amino acids within CCAAT enhancer-binding proteins (C/EBP(alpha) and ... A mitochondrial isozyme of the encoded protein also has been characterized. Click on genes, proteins and metabolites below to ... Wilson HL, McFie PJ, Roesler WJ (2003). "Different transcription factor binding arrays modulate the cAMP responsivity of the ... 2002). "Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site". J. Mol. Biol. ...
Zuo Y, Qiang L, Farmer SR (March 2006). "Activation of CCAAT/enhancer-binding protein (C/EBP) alpha expression by C/EBP beta ... inhibited adipogenesis and repressed induction of the master regulators PPARγ and CCAAT/enhancer-binding protein alpha (CEBPA ... binding protein], PACT (protein activator of the interferon-induced protein kinase), the SMN complex, fragile X mental ... These proteins have three highly positively charged regions, termed AT hooks, that bind the minor groove of AT-rich DNA ...
"A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy ... E3 SUMO-protein ligase PIAS4 is one of several protein inhibitor of activated STAT (PIAS) proteins. It is also known as protein ... Mothers against decapentaplegic homolog 7 and Lymphoid enhancer-binding factor 1. GRCh38: Ensembl release 89: ENSG00000105229 ... "Entrez Gene: PIAS4 Protein inhibitor of activated STAT, 4". Imoto, Seiyu; Sugiyama Kenji; Muromoto Ryuta; Sato Noriko; Yamamoto ...
"A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy ... Eaton EM, Sealy L (August 2003). "Modification of CCAAT/enhancer-binding protein-beta by the small ubiquitin-like modifier ( ... Dobreva G, Dambacher J, Grosschedl R (December 2003). "SUMO modification of a novel MAR-binding protein, SATB2, modulates ... Small ubiquitin-related modifier 3 is a protein that in humans is encoded by the SUMO3 gene. SUMO proteins, such as SUMO3, and ...
Embryonal fyn-associated substrate
CCAAT/enhancer-binding protein alpha), glucocorticoid receptors α and β, and p53. Expression of isoforms 1 and 2 has been ... these tyrosine motifs are bound by the SH2 domains of signaling proteins. Important binding partners for this region include ... allowing the CAS protein to function as a scaffold for other proteins including CRK proteins and C3G, a guanine nucleotide ... All CAS proteins except CASS4 contain a YDYVHL motif within this domain, which is an important binding site for the Src SH2 ...
... found in inflammatory zone 1/resistin-like molecule alpha gene expression by a STAT6 and CCAAT/enhancer-binding protein- ... 2003). "The Secreted Protein Discovery Initiative (SPDI), a Large-Scale Effort to Identify Novel Human Secreted and ... Resistin-like beta is a protein that in humans is encoded by the RETNLB gene. GRCh38: Ensembl release 89: ENSG00000163515 - ... 2003). "Bacterial colonization leads to the colonic secretion of RELMbeta/FIZZ2, a novel goblet cell-specific protein". ...
CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. Ccaat-enhancer-binding proteins ... 1992). "Molecular cloning of two C/EBP-related proteins that bind to the promoter and the enhancer of the alpha 1-fetoprotein ... CCAAT/enhancer-binding protein gamma is a protein that in humans is encoded by the CEBPG gene. The C/EBP family of ... "Entrez Gene: CEBPG CCAAT/enhancer binding protein (C/EBP), gamma". Nishizawa M, Nagata S (1992). "cDNA clones encoding leucine- ...
... studies indicate a role for CCAAT enhancer binding proteins alpha and epsilon (C/EBP alpha and C/EBP epsilon ) and CDP/cut in ... CCAAT/enhancer binding protein (C/EBP), epsilon, also known as CEBPE and CRP1, is a type of ccaat-enhancer-binding protein. ... "Entrez Gene: CEBPE CCAAT/enhancer binding protein (C/EBP), epsilon". Antonson P, Stellan B, Yamanaka R, Xanthopoulos KG (Jul ... Antonson P, Stellan B, Yamanaka R, Xanthopoulos KG (Jul 1996). "A novel human CCAAT/enhancer binding protein gene, C/EBPepsilon ...
CCAAT/enhancer-binding protein alpha, liver activator protein, and D-element-binding protein". Mol. Cell. Biol. 12 (7): 3023-31 ... interaction of CCAAT/enhancer-binding protein with elements flanking the ADH2 TATA box". Gene. 90 (2): 271-9. doi:10.1016/0378- ... 1986). "cDNA and protein structure for the alpha subunit of human liver alcohol dehydrogenase". Biochemistry. 25 (9): 2465-70. ... Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. This gene is ...
Transcription factors, peroxis proliferator-activated receptor γ (PPARγ) and CCAAT enhancer-binding proteins (C/EBPs) are main ... February 1999). "Cross-regulation of C/EBP alpha and PPAR gamma controls the transcriptional pathway of adipogenesis and ... cAMP-responsive element binding protein promotes differentiation, while the activation of PPARγ and C/EBPα is also responsive ... T-cell factor/lymphoid enhancer-binding factor (TCF/LEF), GATA2/3, retinoic acid receptor α, and SMAD6/7 don't affect the ...
Transmembrane protein 251
CCAAT binding factors, or CCAAT enhancer binding proteins were found. The TMEM251 protein is 169 amino acids in length. The ... 71.6% alpha helix. 8.9% turns It is predicted to have two transmembrane helices, of 23 amino acids in length each. The average ... Transmembrane protein 251, also known as C14orf109 or UPF0694, is a protein that in humans is encoded by the TMEM251 gene. One ... No vertebrate TATA binding protein factors, RNA polymerase transcription factor II B, ...
Estrogen receptor alpha
Boruk M, Savory JG, Haché RJ (Nov 1998). "AF-2-dependent potentiation of CCAAT enhancer binding protein beta-mediated ... "Entrez Gene: DBI diazepam binding inhibitor (GABA receptor modulator, acyl-Coenzyme A binding protein)". Kos M, Reid G, Denger ... Chan SW, Hong W (Jul 2001). "Retinoblastoma-binding protein 2 (Rbp2) potentiates nuclear hormone receptor-mediated ... leucine-rich protein 1 Estrogen receptor alpha has been shown to interact with: AKAP13 AHR BRCA1 CAV1 CCNC CDC25B CEBPB COBRA1 ...
Liver X receptor alpha
"Different regulation of the LXRalpha promoter activity by isoforms of CCAAT/enhancer-binding proteins". Biochemical and ... Liver X receptor alpha (LXR-alpha) is a nuclear receptor protein that in humans is encoded by the NR1H3 gene (nuclear receptor ... "The atypical interaction of peroxisome proliferator-activated receptor alpha with liver X receptor alpha antagonizes the ... peroxisome proliferator-activated receptor alpha and liver-X-receptor alpha". Molecular and Cellular Endocrinology. 146 (1-2): ...
Freytag SO, Paielli DL, Gilbert JD (Jul 1994). "Ectopic expression of the CCAAT/enhancer-binding protein alpha promotes the ... "CCAAT/enhancer-binding protein family members recruit the coactivator CREB-binding protein and trigger its phosphorylation". ... CCAAT-enhancer-binding proteins (or C/EBPs) is a family of transcription factors composed of six members, named from C/EBPα to ... CCAAT/enhancer-binding proteins are often involved in growth arrest and differentiation, which has been interpreted to suggest ...
... activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the ... "Extracellular HIV-1 Tat protein induces a rapid and selective activation of protein kinase C (PKC)-alpha, and -epsilon and - ... Protein kinase C eta type is an enzyme that in humans is encoded by the PRKCH gene. Protein kinase C (PKC) is a family of ... "Entrez Gene: PRKCH protein kinase C, eta". Greif H, Ben-Chaim J, Shimon T, et al. (1992). "The protein kinase C-related PKC-L( ...
... by activating a p38 delta mitogen-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha". J. Biol ... "Extracellular HIV-1 Tat protein induces a rapid and selective activation of protein kinase C (PKC)-alpha, and -epsilon and - ... Serine/threonine-protein kinase D3 (PKD3) or PKC-nu is an enzyme that in humans is encoded by the PRKD3 gene. Protein kinase C ... human immunodeficiency virus type 1 Tat protein is associated with an increase in both NF-kappa B binding and protein kinase C ...
Sp1 transcription factor
"Functional cooperation of simian virus 40 promoter factor 1 and CCAAT/enhancer-binding protein beta and delta in ... "Sp1 and Smad proteins cooperate to mediate transforming growth factor-beta 1-induced alpha 2(I) collagen expression in human ... Sp1 and cAMP-response-element-binding protein-binding protein (CBP/p300)". The Biochemical Journal. 339 ( Pt 3) (3): 751-8. doi ... gene is mediated by interactions of Msx1 protein with a multi-protein transcriptional complex containing TATA-binding protein, ...
Glutamate-rich protein 4
"CCAAT/Enhancer-binding Protein Family Members Recruit the Coactivator CREB-binding Protein and Trigger Its Phosphorylation". ... The alpha helix segments span from amino acids 2-9, 21-24, 47-58, 61-94, and 104-111 in the protein sequence. ERICH4 is not ... PMID 12857754.CS1 maint: multiple names: authors list (link) Ramji D.P., Foka P. (2002). "CCAAT/enhancer-binding proteins: ... As suggested by the protein's name, glutamate-rich protein 4, the protein is most highly composed of glutamic acid amino acids ...
Ccaat/Enhancer binding protein (CEBP) cAMP-responsive element binding proteins (CREB) Vertebrate TATA binding protein factor ( ... two alpha-helical segments were predicted. Predictive models made by Phyre2 and SWISS-Model have shown two alpha-helical ... "Protein BLAST: search protein databases using a protein query". blast.ncbi.nlm.nih.gov. Retrieved 2020-03-02. "Genomatix - NGS ... C/EBP homologous protein (CHOP) X-box binding factors (XBBF) TALE homeodomain class recognizing TG motifs (TALE) Human and ...
Chromosome 1 open reading frame 112
Transcription factor assessment indicates many potential TATA-binding protein and CCAAT-enhancer-binding proteins sites, along ... C1orf112 secondary structure is predicted to be predominately alpha helical, with < 5% of the protein composed of beta sheets. ... C1orf112 is predicted to interact with a diverse range of proteins, including multiple mitosis-associated proteins. C1orf112 is ... quality-controlled protein-protein association networks, made broadly accessible". Nucleic Acids Research. 45 (D1): D362-D368. ...
... interaction of CCAAT/enhancer-binding protein with elements flanking the ADH2 TATA box". Gene. 90 (2): 271-9. doi:10.1016/0378- ... Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. GRCh38: ... 2. The primary structure of the gamma 1 protein chain". European Journal of Biochemistry / FEBS. 145 (3): 447-53. doi:10.1111/j ... Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibit high ...
In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... DNA binding by the alpha subunit of RNA polymerase". Science. 262 (5138): 1407-1413. Bibcode:1993Sci...262.1407R. doi:10.1126/ ... Promoters represent critical elements that can work in concert with other regulatory regions (enhancers, silencers, boundary ... CCAAT boxes are common, as they are in many promoters that lack TATA boxes. In addition, the motifs NRF-1, GABPA, YY1, and ...
4.5 Class: beta-Barrel alpha-helix transcription factors. *4.6 Class: TATA binding proteins *4.6.1 Family: TBP ... DNA-binding domain (DBD), which attaches to specific sequences of DNA (enhancer or promoter. Necessary component for all ... I. constitutively active - present in all cells at all times - general transcription factors, Sp1, NF1, CCAAT ... although the consensus binding site for the TATA-binding protein (TBP) is TATAAAA, the TBP transcription factor can also bind ...
Boruk M, Savory JG, Haché RJ (November 1998). "AF-2-dependent potentiation of CCAAT enhancer binding protein beta-mediated ... The role of alpha and beta isoforms of the glucocorticoid receptor]". Arch. Bronconeumol. 38 (9): 436-40. doi:10.1016/S0300- ... the heat shock protein 70 (hsp70) and the protein FKBP4 (FK506-binding protein 4). The endogenous glucocorticoid hormone ... Hulkko SM, Wakui H, Zilliacus J (August 2000). "The pro-apoptotic protein death-associated protein 3 (DAP3) interacts with the ...
"Regulation of the homeodomain CCAAT displacement/cut protein function by histone acetyltransferases p300/CREB-binding protein ( ... matrix attachment region upstream of the T cell receptor beta gene enhancer binds Cux/CDP and SATB1 and modulates enhancer- ... Interleukin1-alpha, and Cyclo-oxygenase 2 (COX2) genes. Genetic data from over 7,600 cancer patients shows that over 1% has the ... "Regulation of the homeodomain CCAAT displacement/cut protein function by histone acetyltransferases p300/CREB-binding protein ( ...
Another aspect of the CCAAT binding motif is the CCAAT/enhancer binding proteins (C/EBPs). They are a group of transcription ... The first domain (A1) contains 20 amino acids that forms an alpha helix that appears significant in its interactions with NF-YB ... Protein specific binding is required for the CCAAT box activation. These proteins are known as CCAAT box binding proteins/CCAAT ... Ramji, Dpiak P.; Foka, Pelagia (10 May 2002). "Review Article: CCAAT/enhancer-binding proteins: structure, function and ...
TFG-TEC binds to the proximal promoter region of the ENO3 gene. Click on genes, proteins and metabolites below to link to ... activator protein 1 and 2, CCAAT box transcription factor/nuclear factor I, and cyclic AMP. Unlike the other enolase genes, ... and two myocyte-specific enhancer-binding factor 1 boxes. Upstream of the first exon lies a TATA-like box and CpG-rich region, ... A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have ...
Multiple Epidermal Growth Factor-like Domains 8
Ccaat/Enhancer Binding Protein Vertebrate TATA binding protein factor CCAAT binding factors Activator-, mediator- and TBP- ... with occasional short alpha helix segments. PELE uses eight different prediction programs to compare and confirm predictions, ... factor 1 RXR heterodimer binding sites GATA binding factors Nuclear receptor subfamily 2 factors Octamer binding protein EGR/ ... proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be ...
DNA damage-inducible transcript 3
It is a member of the CCAAT/enhancer-binding protein (C/EBP) family of DNA-binding transcription factors. The protein functions ... Li G, Mongillo M, Chin KT, Harding H, Ron D, Marks AR, Tabas I (September 2009). "Role of ERO1-alpha-mediated stimulation of ... Qiao D, Im E, Qi W, Martinez JD (June 2002). "Activator protein-1 and CCAAT/enhancer-binding protein mediated GADD153 ... Ramji DP, Foka P (August 2002). "CCAAT/enhancer-binding proteins: structure, function and regulation". The Biochemical Journal ...
For example, CCAAT/enhancer-binding protein β (C/EBPβ), an early adipogenic transcription factor, recruits and requires KMT2D ... Mo R, Rao SM, Zhu YJ (June 2006). "Identification of the MLL2 complex as a coactivator for estrogen receptor alpha". The ... KMT2C and KMT2D are required for the binding of H3K27 acetyltransferases CREB-binding protein (CBP) and/or p300 on enhancers, ... The protein selectively binds enhancer regions based on type of cell and stage of differentiation. During differentiation, ...
... beta-Barrel alpha-helix transcription factors 4.6 Class: TATA binding proteins 4.6.1 Family: TBP 4.7 Class: HMG-box 4.7.1 ... Transcription factors bind to either enhancer or promoter regions of DNA adjacent to the genes that they regulate. Depending on ... Heteromeric CCAAT factors 4.8.1 Family: Heteromeric CCAAT factors 4.9 Class: Grainyhead 4.9.1 Family: Grainyhead 4.10 Class: ... RAV Cdx protein family DNA-binding protein Inhibitor of DNA-binding protein Nuclear receptor, a class of ligand activated ...
Serum response factor
Hanlon M, Sealy L (May 1999). "Ras regulates the association of serum response factor and CCAAT/enhancer-binding protein beta ... and Sp1 interact as components of a multiprotein transcriptional complex required for activity of the human cardiac alpha-actin ... This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the ... Serum response factor has been shown to interact with: ASCC3, ATF6, CEBPB, CREB-binding protein, ELK4, GATA4, GTF2F1, GTF2I, ...
Holland MP, Bliss SP, Berghorn KA, Roberson MS (2004). "A role for CCAAT/enhancer-binding protein beta in the basal regulation ... 2001). "A role for the homeobox protein Distal-less 3 in the activation of the glycoprotein hormone alpha subunit gene in ... Homeobox protein DLX-3 is a protein that in humans is encoded by the DLX3 gene. Dlx3 is a crucial regulator of hair follicle ... Park GT, Denning MF, Morasso MI (2001). "Phosphorylation of murine homeodomain protein Dlx3 by protein kinase C". FEBS Lett. ...
"Modulation of DNA binding properties of CCAAT/enhancer binding protein epsilon by heterodimer formation and interactions with ... Wang D, Westerheide SD, Hanson JL, Baldwin AS (Oct 2000). "Tumor necrosis factor alpha-induced phosphorylation of RelA/p65 on ... Parry GC, Mackman N (Dec 1997). "Role of cyclic AMP response element-binding protein in cyclic AMP inhibition of NF-kappaB- ... Gerritsen ME, Williams AJ, Neish AS, Moore S, Shi Y, Collins T (Apr 1997). "CREB-binding protein/p300 are transcriptional ...
Ccaat-enhancer binding protein KRAB domain zinc finger protein 57 Krüppel-like C2H2 zinc finger factors Octamer binding protein ... The secondary structure of CCDC180 is predicted to be almost completely composed of alpha helices, with only a few predicted ... binding factors Nerve growth factor-induced protein C Myc-associated zinc finger GC-binding factor 2 X-box binding protein 1 ... Coiled-coil domain containing protein 180 (CCDC180) is a protein that in humans is encoded by the CCDC180 gene. This protein is ...
CCAAT enhancer binding protein alpha ELISA Kits | Biocompare.com
Compare CCAAT enhancer binding protein alpha ELISA Kits from leading suppliers on Biocompare. View specifications, prices, ... C/EBP alpha (CCAAT/Enhancer Binding Protein Alpha) BioAssay™ ELISA Kit (Human) ... CCAAT enhancer binding protein alpha ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody- ... Your search returned 119 CCAAT enhancer binding protein alpha ELISA ELISA Kit across 10 suppliers. ...
Impaired translation of CCAAT/enhancer binding protein alpha mRNA in bronchial smooth muscle cells of asthmatic patients
... J ... cells of asthmatic patients have an impaired expression of CCAAT/enhancer binding protein (C/EBP) alpha, which is associated ... CCAAT-Enhancer-Binding Protein-alpha / biosynthesis* * CCAAT-Enhancer-Binding Protein-alpha / immunology ... and 4E binding protein. Results: Compared with healthy control subjects, BSM cells of asthmatic patients proliferate faster ( ...
Growth-dependent inhibition of CCAAT enhancer-binding protein (C/EBP alpha) gene expression during hepatocyte proliferation in...
We have focused on CCAAT enhancer-binding protein (C/EBP alpha), a transcription factor which is highly abundant in normal ... Growth-dependent inhibition of CCAAT enhancer-binding protein (C/EBP alpha) gene expression during hepatocyte proliferation in ... Growth-dependent inhibition of CCAAT enhancer-binding protein (C/EBP alpha) gene expression during hepatocyte proliferation in ... Growth-dependent inhibition of CCAAT enhancer-binding protein (C/EBP alpha) gene expression during hepatocyte proliferation in ...
CCAAT-Enhancer-Binding Protein-alpha | Profiles RNS
"CCAAT-Enhancer-Binding Protein-alpha" by people in this website by year, and whether "CCAAT-Enhancer-Binding Protein-alpha" was ... CCAAT-Enhancer-Binding Protein-alpha*CCAAT-Enhancer-Binding Protein-alpha. *CCAAT Enhancer Binding Protein alpha ... "CCAAT-Enhancer-Binding Protein-alpha" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, ... Below are the most recent publications written about "CCAAT-Enhancer-Binding Protein-alpha" by people in Profiles. ...
CEBPA gene - Genetics Home Reference - NIH
CEBPA Gene - GeneCards | CEBPA Protein | CEBPA Antibody
Protein Coding), CCAAT Enhancer Binding Protein Alpha, including: function, proteins, disorders, pathways, orthologs, and ... Protein Symbol:. P49715-CEBPA_HUMAN. Recommended name:. CCAAT/enhancer-binding protein alpha. Protein Accession:. P49715. ... CEBPA (CCAAT Enhancer Binding Protein Alpha) is a Protein Coding gene. Diseases associated with CEBPA include Leukemia, Acute ... CCAAT enhancer binding protein alpha (C/EBP),expressed at highest level in placenta,also expressed in liver,lung,skeletal ...
CEBPB - CCAAT/enhancer-binding protein beta - Homo sapiens (Human) - CEBPB gene & protein
Its functional capacity is governed by protein interactions and post-translational protein modifications. During early ... Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase ... regulation of eIF2 alpha phosphorylation by heme Source: Reactome. *regulation of interleukin-6 production Source: Ensembl ... "Antioxidant-induced nuclear translocation of CCAAT/enhancer-binding protein beta. A critical role for protein kinase A-mediated ...
CEBPA gene: MedlinePlus Genetics
The CEBPA gene provides instructions for making a protein called CCAAT enhancer-binding protein alpha. Learn about this gene ... The CEBPA gene provides instructions for making a protein called CCAAT enhancer-binding protein alpha. This protein is a ... The other type of mutation blocks the DNA-binding ability of CCAAT enhancer-binding protein alpha. Impaired DNA binding ... The mutations result in a shorter version of CCAAT enhancer-binding protein alpha. This shortened protein is produced from one ...
Impairment of CCAAT Enhancer Binding Proteins alpha (CEBPA) function is a | Nutlin-3 overcomes arsenic trioxide resistance and...
Impairment of CCAAT Enhancer Binding Proteins alpha (CEBPA) function is a. Posted on September 25, 2017. by Billy James ... Impairment of CCAAT Enhancer Binding Proteins alpha (CEBPA) function is a common acquiring in acute myeloid leukemia; even so, ... American blotting Protein ingredients extracted from Trizol (Invitrogen) had been equally packed on 12.5% homogeneous SDS-PAGE ... tissue marrow of the murine style of severe promyelocytic leukemia and discovered markedly reduced degrees of mRNA and proteins ...
CCAAT-Enhancer-Binding Protein-beta Summary Report | CureHunter
A CCAAT-enhancer-binding protein found in LIVER; INTESTINES; LUNG and ADIPOSE TISSUE. It is an important mediator of ... CCAAT-Enhancer-Binding Protein-alpha 6. CCAAT-Enhancer-Binding Protein-beta 7. Interleukin-6 (Interleukin 6) ... Nuclear Proteins: 1752*CCAAT-Enhancer-Binding Proteins: 316*CCAAT-Enhancer-Binding Protein-beta: 377*human CEBPB protein ... CCAAT-Enhancer-Binding Proteins: 316*CCAAT-Enhancer-Binding Protein-beta: 377*human CEBPB protein ...
CCAAT/enhancer binding protein epsilon is a potential retinoid target gene in acute promyelocytic leukemia treatment. - PubMed...
Our data suggest that induction of C/EBPepsilon expression was through the retinoic acid receptor alpha (RARalpha) pathway. ... CCAAT/enhancer binding protein epsilon is a potential retinoid target gene in acute promyelocytic leukemia treatment.. Park DJ1 ... The CCAAT/enhancer binding protein epsilon (C/EBPepsilon) is a nuclear transcription factor expressed predominantly in myeloid ... CCAAT/enhancer binding protein ε is a potential retinoid target gene in acute promyelocytic leukemia treatment ...
Avicularin, a plant flavonoid, suppresses lipid accumulation through repression of C/EBPα-activated GLUT4-mediated glucose...
CCAAT-Enhancer-Binding Protein-alpha / genetics * CCAAT-Enhancer-Binding Protein-alpha / metabolism* ... CCAAT/enhancer-binding protein (C/EBP) α, and aP2 (fatty acid-binding protein 4). In contrast, avicularin did not affect the ... Moreover, the binding of C/EBPα to the promoter region of the GLUT4 gene was repressed by adding avicularin to the medium in ...
Evaluation of the Association Between Arsenic and Diabetes
CCAAT/enhancer binding protein (C/EBP alpha); DMAIII oxide, dimethylarsine oxide; DMAV, dimethylarsinate; HIF1a, hypoxia ... CCAAT/enhancer binding protein (C/EBP alpha); DMAIII oxide, dimethylarsine oxide; DMAV, dimethylarsinate; HIF1a, hypoxia ... 2002). Expression of both peroxisome proliferator-activated receptor-γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα) is ... adipocyte fatty acid binding protein (A-FABP), which is involved in preadipocyte differentiation, and p21, a protein whose ...
Enasidenib induces acute myeloid leukemia cell differentiation to promote clinical response | Blood Journal
CEBPA, CCAAT/enhancer-binding protein alpha; MLL, mixed-lineage leukemia.. Additionally, cytogenetics were overlaid with ... The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha- ... These mutant proteins possess neomorphic enzymatic activity resulting in R-2-hydroxyglutarate (R-2-HG) accumulation.1⇓⇓-4 R-2- ... DNA methyltransferase 3 alpha (DNMT3A) (42%), additional sex combs like 1 (ASXL1) (27%), runt-related transcription factor 1 ( ...
Gene Ontology Classifications
CCAAT/enhancer binding protein (C/EBP), alpha. MGI:99480 Go Annotations as Summary Text (Tabular View) (GO Graph). Summary from ... The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity ... The use of alternative in-frame non-AUG (CUG) and AUG start codons results in several protein isoforms with different lengths. ... This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT ...
Frontiers | Role of Endocrine-Disrupting Engineered Nanomaterials in the Pathogenesis of Type 2 Diabetes Mellitus |...
... alpha serine/threonine kinase; CHOP, CCAAT-enhancer-binding protein homologous protein; JNK, c-Jun N-terminal kinase; GSK 3β, ... CCAAT-Enhancer-Binding Protein Homologous Protein; Cpg-Odns, Cpg Oligodeoxynucleotides; CrNano, Chromium Nanoparticles; CRP, C- ... The cellular metabolism is regulated by various proteins (e.g., protein kinase C), receptors (e.g., receptor tyrosine kinase) ... where it binds to the receptor within the cell and blocks the endogenous hormone from binding. The normal signaling is blocked ...
006447 - B6.129S6(CBA)-Cebpa|tm1Dgt|/J
Cebpa, CCAAT/enhancer binding protein (C/EBP), alpha. Gene Synonym(s). Site of Expression. Following 3-4.5 weeks of poly I:C ... Western blot of whole-cell lysates of adult liver and total bone marrow cells showed that protein was present in mutants.. ... of hematopoietic stem cell repopulating capacity and self-renewal in the absence of the transcription factor C/EBP alpha. ... of hematopoietic stem cell repopulating capacity and self-renewal in the absence of the transcription factor C/EBP alpha. ...
KEGG BRITE: KEGG Orthology (KO) - Ailuropoda melanoleuca (giant panda)
CCAAT/enhancer-binding protein alpha 100469134 CEBPG; CCAAT/enhancer-binding protein gamma 100471463 CEBPE; CCAAT/enhancer- ... CCAAT/enhancer binding protein (C/EBP), alpha K10049 CEBPG; CCAAT/enhancer binding protein (C/EBP), gamma K10051 CEBPE; CCAAT/ ... TATA box-binding protein-like protein 1 100482953 TBP; TATA-box-binding protein 105242046 TBPL2; TATA box-binding protein-like ... GATA-binding protein 1 K17894 GATA2; GATA-binding protein 2 K17895 GATA3; GATA-binding protein 3 K09183 GATA4; GATA-binding ...
Epigenetic differences in cytogenetically normal versus abnormal acute myeloid leukemia.
0/CCAAT-Enhancer-Binding Proteins; 0/CEBPA protein, human; 0/CTNNA1 protein, human; 0/DNA, Neoplasm; 0/DNA-Binding Proteins; 0/ ... 0/Tumor Suppressor Proteins; 0/alpha Catenin; 0/tumor suppressor protein p73; EC 18.104.22.168/FLT3 protein, human; EC 22.214.171.124/fms- ... CCAAT-Enhancer-Binding Proteins / genetics. Case-Control Studies. DNA Methylation. DNA, Neoplasm / genetics. DNA-Binding ... Nuclear Proteins / genetics. Receptors, Estrogen / genetics. Tumor Suppressor Proteins / genetics. U937 Cells. alpha Catenin / ...
Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human...
1996) The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the ... Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ... Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ... Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ...
CCAAT/enhancer binding protein zeta (mouse, aa620-633) Antibody (internal region) - Peptide-affinity purified goat antibody WB,...
CCAAT/enhancer binding protein zeta (mouse, aa620-633) Antibody (internal region), Peptide-affinity purified goat antibody ... CCAAT-box-binding transcription factor; CCAAT/enhancer binding protein alpha (C/EBP) related sequence 1; CCAAT/enhancer-binding ... AF3887a: CCAAT/enhancer binding protein zeta (mouse) Antibody (C-Term). AF3888a: CCAAT/enhancer binding protein zeta (mouse, ... CCAAT/enhancer binding protein zeta (mouse, aa620-633) Antibody (internal region) CCAAT/enhancer binding protein zeta (mouse, ...
Professor Dipak Ramji - People - Cardiff University
Regulation of CCAAT/enhancer binding protein-alpha gene transcription by interleukin-6. Atherosclerosis Supplements 5(1), pp. ... Regulation of CCAAT/enhancer binding protein-alpha gene transcription by interleukin-6. Atherosclerosis Supplements 5(1), pp. ... CCAAT/Enhancer binding proteins: structure, function and regulation. Biochemical Journal 365, pp. 561-575. (10.1042/BJ20020508) ... CCAAT/Enhancer binding proteins: structure, function and regulation. Biochemical Journal 365, pp. 561-575. (10.1042/BJ20020508) ...
Anti-CEBP Alpha/CEBPA antibody [15C8] (ab15047) | Abcam
Mouse monoclonal CEBP Alpha/CEBPA antibody [15C8]. Validated in WB and tested in Mouse, Rat, Human. Cited in 2 publication(s). ... CCAAT Enhancer Binding Protein alpha antibody. *CCAAT/enhancer binding protein (C/EBP), alpha antibody ... C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the ... Eukaryotic Initiation Factor 4E in Nucleophosmin 1 Haploinsufficient Cells Results in Changes in CCAAT Enhancer-binding Protein ...
alkaline earth cation - Ontology Report - Rat Genome Database
CCAAT/enhancer binding protein alpha. 1. 91,363,492. 91,366,164. RGD:6480464. G. Clec3b. C-type lectin domain family 3, member ... S100 calcium binding protein A13. 2. 189,906,022. 189,912,516. RGD:6480464. G. S100a8. S100 calcium binding protein A8. 2. ... S100 calcium binding protein A9. 2. 190,097,436. 190,100,209. RGD:6480464. G. Shc1. SHC adaptor protein 1. 2. 188,745,503. ... LIM and SH3 protein 1. 10. 85,744,662. 85,785,130. RGD:6480464. G. Lrrfip1. LRR binding FLII interacting protein 1. 9. ...
Familial acute myeloid leukemia with mutated CEBPA: MedlinePlus Genetics
The CEBPA gene provides instructions for making a protein called CCAAT enhancer-binding protein alpha. This protein is a ... Absence of the tumor suppressor function of CCAAT enhancer-binding protein alpha is believed to disrupt the regulation of blood ... Dominant-negative mutations of CEBPA, encoding CCAAT/enhancer binding protein-alpha (C/EBPalpha), in acute myeloid leukemia. ... gene mutations identified in leukemia cells generally decrease the DNA-binding ability of CCAAT enhancer-binding protein alpha ...
Biology | Free Full-Text | Groundsel Bush (Baccharis halimifolia) Extract Promotes Adipocyte Differentiation In Vitro and...
... increased the adiponectin protein levels in a time-dependent manner, and substantially attenuated the TNF-alpha-induced ... Protein levels of two of these adipogenic markers (aP2 and adiponectin) were examined and found to be induced by GB treatment. ... and protein abundance, respectively. In differentiating 3T3-L1 adipocytes, GB enhanced lipid accumulation and increased ... CCAAT/enhancer binding protein (C/EBP) alpha (CEBPα; PPM04674), Sterol regulatory element binding transcription factor 1 ( ...
Molecular mechanisms of gene activation and gene expression mediated by CCAAT/enhancer binding proteins
The transcription factor CCAAT/Enhancer-Binding Protein alpha (C/EBPa) coordinates proliferation arrest and differentiation of ... Der Transkriptionsfaktor CCAAT/Enhancer-Binding Protein alpha (C/EBPa) koordiniert Proliferationshemmung und Differenzierung ... Molecular mechanisms of gene activation and gene expression mediated by CCAAT/enhancer binding proteins. ... but by direct protein-protein interactions that abolish the binding of C/EBPa to DNA. This mechanism of transcriptional ...
Dominance (genetics) - Wikipedia
March 2001). "Dominant-negative mutations of CEBPA, encoding CCAAT/enhancer binding protein-alpha (C/EBPalpha), in acute ... an aggregate of multiple copies of the same protein, otherwise known as a homomultimeric protein or homooligomeric protein. In ... For example, in humans the Hb gene locus is responsible for the Beta-chain protein (HBB) that is one of the two globin proteins ... In fact, the first study reporting a mutant protein inhibiting the normal function of a wild-type protein in a mixed multimer ...
Basic leucine zipper proteins Gene Family | HUGO Gene Nomenclature Committee
CCAAT/enhancer binding protein alpha. CEBP. C/EBP-alpha. 19q13.11. CEBPB CCAAT/enhancer binding protein beta. TCF5. LAP, CRP2, ... CCAAT/enhancer binding protein gamma. GPE1BP, IG/EBP-1. 19q13.11. CREBL2 cAMP responsive element binding protein like 2. ... CCAAT/enhancer binding protein epsilon. CRP1. 14q11.2. CEBPG ... CCAAT/enhancer binding protein delta. CRP3, CELF, C/EBP-delta, ... cAMP responsive element binding protein 1. 2q33.3. CREB3 cAMP responsive element binding protein 3. LZIP, Luman, sLZIP. 9p13.3 ...
Recombinant Human CCAAT/enhancer Binding Protein(C/EBP) α, His-tagged CEBPA-94H - Creative BioMart
CEBPA; C/EBP-alpha; CEBP; CCAAT/enhancer binding protein alpha; CCAAT/enhancer binding protein (C/EBP), alpha. ... CEBPA CCAAT/enhancer binding protein (C/EBP), alpha [ Homo sapiens ]. Synonyms:. ... Recombinant Human CCAAT/enhancer Binding Protein(C/EBP) α, His-tagged. Download Datasheet See All CEBPA Products. Bring this ... CCAAT/enhancer binding protein(C/EBP) α is a family of transcription factors that all contain a highly conserved, basic-leucine ...
CEBPAGenesCytosine-cytosine-adenosine-adenosine-thymidinePromoterBZIPEpsilonCEBPKinaseLiverEBPsHematopoieticCEBPBMetabolismLeucineGene expressionGammaDifferentiationSecondary granule proteinsHeterodimersExpressionReceptorsBasalEukaryoticChromatinPeptidesHomodimersCellsAmino AcidsTumor necroTranscription factor activityPromotersTranscriptional activityFactorEBPalphaAcuteForkhead boxDistalViralRegulatoryActivationRegulateModulateCREBMutation
- We also analyzed levels of proteins that control translation of CEBPA mRNA, namely heterogeneous nuclear ribonucleoprotein E2, calreticulin, eukaryotic translation initiation factor (eIF4E), and 4E binding protein. (nih.gov)
- The CEBPA gene provides instructions for making a protein called CCAAT enhancer-binding protein alpha. (nih.gov)
- This shortened protein is produced from one copy of the CEBPA gene in each cell, and it is believed to interfere with the tumor suppressor function of the normal protein produced from the second copy of the gene. (nih.gov)
- The somatic CEBPA gene mutations that have been identified in leukemia cells generally decrease the DNA-binding ability of CCAAT enhancer-binding protein alpha. (nih.gov)
- CEBPA (CCAAT Enhancer Binding Protein Alpha) is a Protein Coding gene. (genecards.org)
- Reciprocally E2F1 blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. (genecards.org)
- Synthetic peptide corresponding to Mouse CEBP Alpha/CEBPA aa 1-14. (abcam.com)
- CEBPA gene mutations that cause familial acute myeloid leukemia with mutated CEBPA result in a shorter version of CCAAT enhancer-binding protein alpha. (medlineplus.gov)
- Binds to the CYP3A4 promoter and activates its transcription in cooperation with CEBPA. (genecards.org)
- Most CEBPA DM patients have a classic biallelic mutant combination with an N-terminal mutation leading to production of p30 protein plus a C-terminal loss-of-function in-frame indel mutation ( CEBPA Classic-DM ), but approximately one-third of cases have one or more non-classic mutations, with diverse combinations reported, and there is little information on the consequences of such mutants. (haematologica.org)
- The CEBPA gene encodes CCAAT/enhancer binding protein-α (C/EBPα), a basic leucine zipper (bZIP) transcription factor that is essential for hematopoietic stem cell regulation and myeloid development. (haematologica.org)
- Background and aims: CCAAT/enhancer-binding protein alpha (CEBPA) is a transcription factor involved in adipogenesis and energy homeostasis. (diva-portal.org)
- Caloric restriction reduces CEBPA protein expression in patients with metabolic syndrome (MetS). (diva-portal.org)
- MTL-CEBPA is the first therapy to specifically up-regulate CCAAT/enhancer binding protein alpha (C/EBP-α), a transcription factor that acts as a master regulator of myeloid cell lineage determination and differentiation. (businesswire.com)
- Mutations of CCAAT enhancer binding protein alpha (CEBPA), a tumor suppressor, are found in about 10% of human AML patients. (oncologynurseadvisor.com)
- We have focused on CCAAT enhancer-binding protein (C/EBP alpha), a transcription factor which is highly abundant in normal liver and is considered to regulate expression of many genes, including some involved in energy metabolism (S. L. McKnight, M. D. Lane, and S. Gluecksohn-Walsh. (asm.org)
- This protein is a transcription factor, which means that it attaches (binds) to specific regions of DNA and helps control the activity (expression) of certain genes. (nih.gov)
- This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. (genecards.org)
- Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. (genecards.org)
- Binds directly to the consensus DNA sequence 5-T[TG]NNGNAA[TG]-3 acting as an activator on distinct target genes (PubMed:11242107). (genecards.org)
- To regulate gluconeogenesis, functionally cooperates with FOXO1 binding to IRE-controlled promoters and regulating the expression of target genes such as PCK1 or G6PC. (genecards.org)
- Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. (uniprot.org)
- Avicularin decreased the intracellular triglyceride level along with a reduction in the expression of adipogenic genes such as peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein (C/EBP) α, and aP2 (fatty acid-binding protein 4). (nih.gov)
- The promoter elements of many monocyte-specific genes contain C/EBP binding sites, including macrophage inflammatory protein 1 alpha, tumor necrosis factor alpha ( 32 ), IL-6 ( 6 , 27 , 38 ), and IL-8 ( 27 , 36 ). (asm.org)
- 2016. The role of mitogen-activated protein kinases and sterol receptor coactivator-1 in TGF-β-regulated expression of genes implicated in macrophage cholesterol uptake . (cardiff.ac.uk)
- C/EBP-β and -δ and ADD1/SREBP1 induce the expression of PPARγ, which triggers the adipogenic process by transactivation of adipose-specific genes involved in lipid storage and metabolism, such as aP2 (fatty acid binding protein), PEPCK (phosphoenolpyruvate carboxykinase), AOX (acyl-CoA oxidase) and LPL (lipoprotein lipase) ( 7 , 8 ). (diabetesjournals.org)
- The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. (wikidoc.org)
-  Genes coding for transporter proteins that confer multidrug resistance to the cells have also been found to be activated by CEBPB. (wikidoc.org)
- 10 CCAAT/enhancer-binding proteins (C/EBPs) binding motifs are identified in the promoter regions of most acute-phase protein genes. (ahajournals.org)
- Differentially expressed genes were found to be involved in several pathways related to preadipocyte differentiation that have been extensively studied, including glycerolipid metabolism, and the mammalian target of rapamycin, peroxisome proliferator-activated receptor, and mitogen-activated protein kinase signaling pathways. (g3journal.org)
- C/EBP proteins interact with the CCAAT (cytosine-cytosine-adenosine-adenosine-thymidine) box motif, which is present in several gene promoters. (wikipedia.org)
- In this study, the role of hypoxia-inducible factor-1 (HIF-1) signaling in the regulation of CCAAT (cytosine-cytosine-adenosine-adenosine-thymidine)/enhancer-binding protein-β (C/EBPβ) and the receptor activator of nuclear factor kappa-B ligand (RANKL) expressions in immortalized human periodontal ligament (PDL) cells was investigated. (nii.ac.jp)
- Reporter gene studies showed that C/EBPepsilon promoter/enhancer activity increased in a retinoid-dependent fashion via the retinoic acid response element (RARE) present in the promoter region of C/EBPepsilon. (nih.gov)
- Transactivational abilities of C/EBPε promoter/enhancer. (nih.gov)
- a ) Schema of CAT reporter constructs with putative retinoic acid response element from C/EBPε (RARE C/EBPε ), mutant RARE C/EBPε , 0.5-kb C/EBPε promoter/enhancer region, and mutant 0.5-kb C/EBPε promoter/enhancer region. (nih.gov)
- b ) Retinoid-induced transactivation of C/EBPε promoter/enhancer in COS-1 cells. (nih.gov)
- The transactivation of C/EBPε promoter/enhancer CAT reporters by RARα, mutant RARα (RARα403), and PML/RARα were compared in either the presence (hatched bars) or absence (solid bars) of ATRA (10 -6 M). The -fold induction of transactivation is calculated relative to that of pCMX transfected without ATRA treatment. (nih.gov)
- Moreover, the binding of C/EBPα to the promoter region of the GLUT4 gene was repressed by adding avicularin to the medium in 3T3-L1 cells, as demonstrated by the results of a chromatin immunoprecipitation assay. (nih.gov)
- Thus, HIV-1 LTR ATF/CREB binding site sequence variation may modulate cellular signaling at the viral promoter through the C/EBP pathway. (asm.org)
- Interaction of hepatitis B viral X protein and CCAAT/enhancer-binding protein alpha synergistically activates the hepatitis B viral enhancer II/pregenomic promoter. (wikipedia.org)
- Pitx2 expression caused an upregulation of miR-200c and chromatin immunoprecipitation assays revealed endogenous Pitx2 binding to the miR-200c/141 promoter. (biologists.org)
- Transcription factor that is thought to act as a pioneer factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites (By similarity). (genecards.org)
- binds to and activates transcription from the G6PC promoter. (genecards.org)
- Binds to the CYP3A7 promoter together with members of the CTF/NF-I family. (genecards.org)
- In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. (wikidoc.org)
- Systematic deletion assay of the promoter demonstrated that the region (−239 to −210 bp) containing a putative CCAAT/enhancer-binding protein (C/EBP) binding site was necessary. (ahajournals.org)
- Conclusions- IL-6 increases hepatic PAI-1 expression mediated by the −232- to −210-bp region of the promoter containing a C/EBPδ binding site. (ahajournals.org)
- 3 PAI-1 promoter contains transcription factor binding sites responsible for inducible expressions. (ahajournals.org)
- Three of the SNPs in the promoter region of COX-2 gene create at least three putative transcription factor binding sites and eliminate CCAAT/enhancer binding protein alpha (C/EBP alpha) and NF-kappa B binding sites. (nih.gov)
- Surprisingly, PPAR response elements in YWHAE promoter were not required for upregulation by PPARδ, whereas a CCAAT/enhancer binding protein (C/EBP) site located at −160/−151 bp regulated both basal and PPARδ-dependent promoter activity. (ahajournals.org)
- The Basic Leucine Zipper Domain ( bZIP domain ) is found in many DNA binding eukaryotic proteins. (genenames.org)
- The CCAAT/enhancer (C/EBP) family of basic-leucine zipper (bZIP) transcription factors is a multifaceted highly-regulated system for gene regulation. (springer.com)
- The protein encoded by this intronless gene is a bZIP transcription factor that can bind as a homodimer to certain DNA regulatory regions. (wikidoc.org)
- The most common C-terminal mutations are in-frame indels in the bZIP DNA binding domain (DBD) or leucine zipper domain (LZD) that lead to loss of the ability to bind to DNA or dimerize, classified here as C-terminal loss-of-function (C-LOF). (haematologica.org)
- CCAAT/enhancer binding protein epsilon is a potential retinoid target gene in acute promyelocytic leukemia treatment. (nih.gov)
- The CCAAT/enhancer binding protein epsilon (C/EBPepsilon) is a nuclear transcription factor expressed predominantly in myeloid cells and implicated as a potential regulator of myeloid differentiation. (nih.gov)
- Lane 1: mouse mouse lysates Lane 2: mouse heart lysates probed with Anti Phospho-CEBP alpha (Thr222/226) Polyclonal Antibody, Unconjugated (ABIN683563) at 1:200 in 4 °C. Followed by conjugation to secondary antibody at 1:3000 90min in 37 °C. Predicted band 45kD. (antikoerper-online.de)
- It can also form heterodimers with the related proteins CEBP-alpha , CEBP-delta , and CEBP-gamma . (wikidoc.org)
- 2015. Protein Kinase C is involved in the induction of ATP-Binding cassette transporter A1 expression by liver X receptor/retinoid X receptor agonist in human macrophages . (cardiff.ac.uk)
- Therapeutic potential of panduratin A, LKB1-dependent AMP-activated protein kinase stimulator, with activation of PPARalpha/delta for the treatment of obesity. (addgene.org)
- protect against high-fat diet-induced obesity by regulating lipid metabolism and activating AMP-activated protein kinase. (addgene.org)
- JTW-medicated serum treatment increased glucose uptake, up-regulated levels of phosphorylated adenosine 5′-monophoshate-activated protein kinase (p-AMPK), and stimulated expression and translocation of glucose transporter 4 (GLUT4). (portlandpress.com)
- p38 Mitogen-activated protein kinase (MAPK) is highly activated in human primary hepatocytes but barely in human hepatoma cell lines including HepG2 cells. (aspetjournals.org)
- Growth-dependent inhibition of CCAAT enhancer-binding protein (C/EBP alpha) gene expression during hepatocyte proliferation in the regenerating liver and in culture. (asm.org)
- Using Northern (RNA) blot analysis, we have examined the expression of C/EBP alpha mRNA during liver regeneration and in primary cultures of hepatocytes. (asm.org)
- Run-on transcription analysis is in agreement with the Northern blot data, thus suggesting that C/EBP alpha is transcriptionally regulated in regenerating liver. (asm.org)
- Western blot of whole-cell lysates of adult liver and total bone marrow cells showed that protein was present in mutants. (jax.org)
- Liver X receptor alpha (LXR-alpha) is a nuclear receptor protein that in humans is encoded by the NR1H3 gene (nuclear receptor subfamily 1, group H, member 3). (wikipedia.org)
- The liver X receptors, LXRα (this protein) and LXRβ, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. (wikipedia.org)
- Liver X receptor alpha has been shown to interact with EDF1 and Small heterodimer partner. (wikipedia.org)
- To evaluate this hypothesis, we focused on two molecules with known oncofetal properties in the liver, Yes-associated protein-1 (YAP1) and Insulin-like growth factor-2 RNA-binding protein-3 (IGF2BP3). (bmj.com)
- Liver tumors escape negative control of proliferation via PI3K/Akt-mediated block of C/EBP alpha growth inhibitory activity. (wikipedia.org)
- Lack of the lysosomal membrane protein, GLMP, in mice results in metabolic dysregulation in liver. (uio.no)
- The CCAAT/enhancer binding protein family (C/EBP) are transcription factors that play integral roles in the development and function of many organ systems, including hematopoietic cells, adipose tissues, and liver. (zfin.org)
- CCAAT-enhancer-binding proteins (or C/EBPs) is a family of transcription factors composed of six members, named from C/EBPα to C/EBPζ. (wikipedia.org)
- Once bound to DNA, C/EBPs can recruit so-called co-activators (such as CBP) that in turn can open up chromatin structure or recruit basal transcription factors. (wikipedia.org)
- The dimerization is necessary to enable C/EBPs to bind specifically to DNA through a palindromic sequence in the major groove of the DNA. (wikipedia.org)
- This process of adipocyte differentiation and development is controlled by a number of transcription factors that include the nuclear receptor peroxisome proliferator-activated receptor γ (PPARγ) ( 3 , 4 ), the family of CCAAT enhancer binding proteins (C/EBPs) ( 5 ) and the basic helix-loop-helix leucine zipper transcriptional factor ADD1/SREBP1 ( 6 ). (diabetesjournals.org)
- 2004. Enhancement of hematopoietic stem cell repopulating capacity and self-renewal in the absence of the transcription factor C/EBP alpha. (jax.org)
- Following 3-4.5 weeks of poly I:C treatment, deletion efficiency is greater than 95% in hematopoietic tissues, and C/EBPalpha protein is undetectable in bone marrow. (jax.org)
- However, there are some mutations leading to an aberrant activation of proteins that have a crucial effect on hematopoietic progenitor cell proliferation and differentiation. (thefreelibrary.com)
- A preparation of hematopoietic progenitor cells (HPCs) from a haploidentical donor that have been depleted of T-cell receptor (TCR) alpha and beta (TCRa/b+) as well as CD19-positive (CD19+) cells, that can potentially be used for immune reconstitution purposes. (cancer.gov)
- The TCR alpha/beta/CD19-depleted HPCs are used for allogeneic hematopoietic cell transplantation (HCT) and may allow for rapid and sustained engraftment, rapid immune reconstitution, and may prevent or reduce graft-versus-host disease (GvHD). (cancer.gov)
- CD47, also called integrin-associated protein (IAP), is a tumor-associated antigen (TAA) expressed on normal, healthy hematopoietic stem cells (HSC) and overexpressed on the surface of a variety of cancer cells. (cancer.gov)
- CCAAT/enhancer-binding protein beta is a protein that in humans is encoded by the CEBPB gene . (wikidoc.org)
- C/EBP proteins are involved in different cellular responses, such as in the control of cellular proliferation, growth and differentiation, in metabolism, and in immunity. (wikipedia.org)
- FoxO1/3a proteins belong to the forkhead box O subfamily of transcription factors that regulate multiple cellular functions from proliferation, metabolism, life span, and stress resistance to gluconeogenesis ( 12 - 14 ). (diabetesjournals.org)
- The corepressor NCoR1 antagonizes PGC-1alpha and estrogen-related receptor alpha in the regulation of skeletal muscle function and oxidative metabolism. (addgene.org)
- C/EBP proteins are all members of the b-ZIP family of transcription factors and share a highly homologous carboxy terminus that contains the basic and leucine zipper protein domains. (asm.org)
- The different C/EBP family members homo- and heterodimerize through their leucine zipper regions and bind to their cognate DNA sequences through the corresponding basic regions. (asm.org)
- One part of the domain contains a region that mediates sequence specific DNA binding properties and the leucine zipper that is required to hold together (dimerize) two DNA binding regions. (genenames.org)
- CCAAT/enhancer binding protein(C/EBP) α is a family of transcription factors that all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. (creativebiomart.net)
- a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. (creativebiomart.net)
- This domain is involved in dimerization and DNA binding, as are other transcription factors of the leucine zipper domain-containing family (c-Fos and c-jun). (wikipedia.org)
- Podust LM, Krezel AM, Kim Y. Crystal structure of the CCAAT box/enhancer-binding protein beta activating transcription factor-4 basic leucine zipper heterodimer in the absence of DNA. (springer.com)
- CCAAT/enhancer binding protein (C/EBP)ε, a member of the leucine zipper family of transcription factors, is expressed primarily in myeloid cells, and its knockout mouse model possesses distinctive defects, including a lack of neutrophil secondary granule proteins. (rupress.org)
- This drop in C/EBP alpha gene expression in response to activation of hepatocyte growth is consistent with the proposal that C/EBP alpha has an antiproliferative role to play in highly differentiated cells (R. M. Umek, A. D. Friedman, and S. L. McKnight, Science 251: 288-292, 1991). (asm.org)
- Impaired DNA binding interferes with the protein's ability to regulate gene expression and impairs its tumor suppressor function. (nih.gov)
- In fact, C/EBP proteins are intimately involved in the regulation of myelocytic/monocytic gene expression. (asm.org)
- Oil Red O neutral lipid staining, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and immunoblotting were used to measure GB effects on lipid accumulation, gene expression, and protein abundance, respectively. (mdpi.com)
- In mature adipocytes, GB reduced the gene expression of resistin, a pro-inflammatory endocrine factor, increased the adiponectin protein levels in a time-dependent manner, and substantially attenuated the TNF-alpha-induced reduction in adiponectin. (mdpi.com)
- Chakrabarty A, Roberts MR. Ets-2 and C/EBP-beta are important mediators of ovine trophoblast Kunitz domain protein-1 gene expression in trophoblast. (springer.com)
- As the transition to extrauterine life at birth alters the proportions of type I and II alveolar epithelial cells (AECs), our aim was to determine the effect of mild preterm birth on AECs and surfactant protein (SP) gene expression. (nature.com)
- The 3 isotypes identified in vertebrates, PPARα (NR1C1), PPARδ (NR1C2), and PPARγ (NR1C3), regulate target gene expression by binding to specific PPAR response elements (PPREs) as a heterodimer with a retinoid X receptor. (ahajournals.org)
- The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. (genecards.org)
- Hepatocyte nuclear factor 3-gamma (HNF-3G), also known as forkhead box protein A3 (FOXA3) or transcription factor 3G (TCF-3G) is a protein that in humans is encoded by the FOXA3 gene . (wikipedia.org)
- Wnt signaling stimulates osteoblastogenesis of mesenchymal precursors by suppressing CCAAT/enhancer-binding protein alpha and peroxisome proliferator-activated receptor gamma. (nih.gov)
- The TCR alpha/beta/CD19-depleted HPCs contain high amounts of natural killer (NK) cells, gamma/delta T-cells, CD34+ stem cells, monocytes, and dendritic cells (DCs), while devoid of alpha/beta T cells and CD19-positive B cells. (cancer.gov)
- We have identified and characterized putative zebrafish orthologs of mammalian C/EBP alpha, beta, gamma, and delta using low-stringency hybridization screening and computer searches of the GenBank EST database. (zfin.org)
- Experiments with knock-out mice have demonstrated that CCAAT-enhancer binding protein-alpha is essential for the functioning and differentiation of HEPATOCYTES and ADIPOCYTES. (jefferson.edu)
- CCAAT enhancer-binding protein alpha is involved in the maturation (differentiation) of certain blood cells. (nih.gov)
- 10(-6) M). In addition, forced expression of C/EBPepsilon in the U937 myelomonoblastic leukemia cells mimicked terminal granulocytic differentiation, including morphologic changes, increased CD11b/CD66b expression, and induction of secondary granule protein expression. (nih.gov)
- The transcription factor CCAAT/Enhancer-Binding Protein alpha (C/EBPa) coordinates proliferation arrest and differentiation of myeloid progenitors and adipocytes. (hu-berlin.de)
- In this issue of Blood , Bartels and colleagues demonstrate that acetylation of the transcription factor CCAAT enhancer binding protein ε (C/EBPε) is essential for terminal neutrophil granulocyte differentiation. (bloodjournal.org)
- Acetylation on K121 and K198 is essential for terminal differentiation of neutrophils and expression of secondary granule proteins. (bloodjournal.org)
- CDDO induces granulocytic differentiation of myeloid leukemic blasts through translational up-regulation of p42 CCAAT enhancer binding protein alpha. (semanticscholar.org)
- Thus, our objective was to determine a) the effects of mild preterm birth, in the absence of sustained ventilatory support, on the differentiation of AECs and surfactant protein mRNA expression and b) the persistence of these changes into early postnatal life. (nature.com)
Secondary granule proteins2
- Neutrophil-specific granule deficiency (SGD) is a rare disorder characterized by recurrent pyogenic infections, defective neutrophil chemotaxis and bactericidal activity, and lack of neutrophil secondary granule proteins. (rupress.org)
- The five reported cases consistently describe pleiotropic characteristics, including lack of secondary granule proteins and defensins, abnormalities in neutrophil migration and disaggregation, atypical nuclear morphology, and impaired bactericidal activity ( 1 - 11 ). (rupress.org)
- C/EBP family consist of several related proteins, C/EBP α, β, γ, δ, that form homodimers and that form heterodimers with each other. (creativebiomart.net)
- Bronchial smooth muscle (BSM) cells of asthmatic patients have an impaired expression of CCAAT/enhancer binding protein (C/EBP) alpha, which is associated with increased proliferation. (nih.gov)
- Cultures of hepatocytes on rat tail collagen in the presence or absence of EGF clearly show that within 3 h, EGF depresses C/EBP alpha mRNA expression and that this effect is substantially greater by 4 h. (asm.org)
- Our data suggest that induction of C/EBPepsilon expression was through the retinoic acid receptor alpha (RARalpha) pathway. (nih.gov)
- While C/EBP proteins are expressed in many human tissues, high levels of C/EBP mRNA and protein expression are limited to only a few cell types, including cells of the myeloid lineage. (asm.org)
- Abgent has over fifteen years of experience producing recombinant proteins in E. coli and mammalian cells (CHO and HEK293, etc), and we have added a powerful yeast expression platform to our menu of services. (abgent.com)
- With state-of-the art molecular biology and protein biochemistry labs, we work with our clients to rapidly evaluate in parallel to identify the optimal expression system for candidate proteins. (abgent.com)
- Expression of C/EBPε is restricted primarily to the myeloid lineage in 4 protein isoforms of 32, 30, 27, and 14 kDa. (bloodjournal.org)
- Restoration of LXR-alpha expression/function within a psoriatic lesion may help to switch the transition from psoriatic to symptomless skin. (wikipedia.org)
- In familial MDS/AML, mutations change the coding sequence of a gene to generate a mutant protein with altered activity or introduce frameshifts or stop codons or disrupt regulatory elements to alter protein expression. (jci.org)
- Molecular cloning, sequence, and expression patterns of the human gene encoding CCAAT/enhancer binding protein alpha (C/EBP alpha). (wikipedia.org)
- Brown fat-mediated thermogenesis and hepatic gluconeogenesis are both induced through expression of PGC-1 alpha, which then docks on a variety of transcription factor targets. (dana-farber.org)
- The nuclear factor for IL-6 expression (NF-IL6) was discovered as a member of the CCAAT-enhancer binding proteins (C/EBP) in 1990, from which it derived its current name: C/EBP-β (Akira et al. (springer.com)
- 1990 ). C/EBP-β was first found to bind to an interleukin 1 (IL1) responsive element necessary for IL-6 expression (Akira et al. (springer.com)
- Expression pattern of the CCAAT/enhancer-binding proteins C/EBP-alpha, C/EBP-beta and C/EBP-delta in the human placenta. (springer.com)
- Lala-Tabbert N, Fu D, Wiper-Bergeron N. Induction of CCAAT/enhancer-binding protein beta expression with the phosphodiesterase inhibitor isobutylmethylxanthine improves myoblast engraftment into dystrophic muscle. (springer.com)
- Marchildon F, Lamarche E, Lala-Tabbert N, St-Louis C, Wiper-Bergeron N. Expression of CCAAT/enhancer binding protein beta in muscle satellite cells inhibits myogenesis in cancer cachexia. (springer.com)
- It was reported that the −82T→C substitution within the CYP2B6*22 allele creates a functional CCAAT/enhancer-binding protein (C/EBP) binding site and enhances the basal expression of the CYP2B6 gene. (aspetjournals.org)
- On the other hand, this synergism was attenuated by disrupting the C/EBP binding site or knocking down C/EBPα expression. (aspetjournals.org)
- Sequence analysis of these mutations purported that a number of them may influence CYP2B6 expression by altering consensus transcription factor binding sites. (aspetjournals.org)
- reported that the −82T→C, a mutation within the CYP2B6*22 allele, enhances the basal expression of CYP2B6 by introducing a functional CCAAT/enhancer-binding protein (C/EBP) binding site and shifting the transcription start site downstream. (aspetjournals.org)
- To that end we are applying genome-wide expression analysis, chromatin immunoprecipitation, protein biochemistry, proteomics and functional screens. (stanford.edu)
- Consistent with these findings, L-165,041 increased 14-3-3ε mRNA and protein level, whereas PPARδ small interfering RNA suppressed both basal and L-165,041-dependent YWHAE transcription and 14-3-3ε protein expression. (ahajournals.org)
- Intriguingly, activation or knock down of endogenous PPARδ regulated C/EBPβ protein expression. (ahajournals.org)
- Expression pattern of the transcription factor CCAAT enhancer binding protein alpha (C/EBPalpha) in an E9.5 mouse embryo. (ehu.es)
- This mechanism relies on cargo recruitment to autophagosomes by receptors that bind to both the ubiquitin-like AUTOPHAGY8 (ATG8) protein through ATG8-interacting motifs (AIMs) and to the cargo to be degraded. (medworm.com)
- In plants, two autophagy cargo receptors, ATG8-interacting protein 1 (ATI1) and 2 (ATI2), were identified early on, but their molecular properties remain poorly understood. (medworm.com)
- Most importantly, sequence variation at the ATF/CREB binding site affected basal LTR activity as well as LTR function following interleukin-6 stimulation, a treatment that leads to increases in C/EBP activation. (asm.org)
- Selective autophagy has emerged as an important mechanism by which eukaryotic cells control the abundance of specific proteins. (medworm.com)
- Electrophoretic mobility shift assay and chromatin immunoprecipitation assay demonstrated that C/EBPα, C/EBPβ, and C/EBPδ were involved in protein-DNA complex formation in intact cells. (ahajournals.org)
- Check out links to articles that cite our custom service antibodies, peptides, and proteins in major peer-reviewed journals, organized by research category. (abgent.com)
- In addition, CREB-1 homodimers bind to the ATF/CREB site and recruit C/EBP dimers to their cognate weak binding sites. (asm.org)
- C/EBP alpha mRNA levels decrease 60 to 80% within 1 to 3 h after partial hepatectomy as the cells move from G0 to G1 and decrease further when cells progress into S phase. (asm.org)
- Liu M, Tu X, Ferrari-Amorotti G, Calabretta B, Baserga R. Downregulation of the upstream binding factor1 by glycogen synthase kinase3beta in myeloid cells induced to differentiate. (jefferson.edu)
- Absence of the tumor suppressor function of CCAAT enhancer-binding protein alpha is believed to disrupt the regulation of blood cell production, leading to the uncontrolled production of abnormal cells that occurs in acute myeloid leukemia. (nih.gov)
- Recent observations have shown two CCAAT/enhancer binding protein (C/EBP) binding sites to be critically important for efficient human immunodeficiency virus type 1 (HIV-1) replication within cells of the monocyte/macrophage lineage, a cell type likely involved in transport of the virus to the brain. (asm.org)
- Additional studies indicated that these two C/EBP binding sites were required for replication of an infectious HIV-1 molecular clone in the U-937 cell line as well as in primary cells of the monocyte/macrophage lineage. (asm.org)
- 2009). "Differential binding and co-binding pattern of FOXA1 and FOXA3 and their relation to H3K4me3 in HepG2 cells revealed by ChIP-seq" . (wikipedia.org)
- This protein is expressed in the mammalian nervous system and plays a significant role in the development and function of nerve cells. (wikipedia.org)
- CCAAT/enhancer binding protein beta is expressed in satellite cells and controls myogenesis. (springer.com)
- Although the mechanism of action has not been elucidated, following subcutaneous administration, CD44 targeted agent SPL-108 binds to CD44 and prevents the activation of various CD44-mediated signal transduction pathways, which may lead to reduced proliferation of CD44-expressing tumor stem cells. (cancer.gov)
- Upon administration, ALX148 binds to CD47 expressed on tumor cells and prevents the interaction of CD47 with its ligand SIRPa, a protein expressed on phagocytic cells. (cancer.gov)
- This induces pro-phagocytic signaling mediated by the binding of the pro-phagocytic signaling protein calreticulin (CRT), which is specifically expressed on the surface of tumor cells, to low-density lipoprotein (LDL) receptor-related protein (LRP), expressed on macrophages. (cancer.gov)
- 13 Statins can modify fibrinolytic potential of endothelial cells via inhibition of geranylgeranylated Rho protein 14 and act on PAI-1 transcription 12 and inhibit inflammatory transcription factors. (ahajournals.org)
- The antiapoptotic PPARδ might affect different proteins in endothelial cells, with 14-3-3 proteins being key candidates because of their ability to promote cell survival. (ahajournals.org)
- Because 14-3-3 proteins and 14-3-3ε are antiapoptotic and antiinflammatory molecules in endothelial cells, they may play an important role in atherothrombosis. (ahajournals.org)
- The DNA binding region comprises a number of basic amino acids such as arginine and lysine. (genenames.org)
- Hypoxia had no effects on the secretion of interleukin (IL)-1β, IL-6, IL-8, IL-17A, tumor necrosis factor-alpha, macrophage migration inhibitory factor, monocyte chemoattractant protein-1, and macrophage colony-stimulating factor in the culture media. (nii.ac.jp)
Transcription factor activity1
- Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and sequence-specific DNA binding . (genecards.org)
- C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. (abcam.com)
- 1 Phosphorylation of C/EBPε on threonine 75, which is located in the transactivation domain, leads to increased DNA binding and transcriptional activity, and sumolyation of a lysine residue 121 (K121) within repression domain I enhances transcriptional activation by C/EBPε. (bloodjournal.org)
- The predicted frame shift results in a truncation of the 32-kD major C/EBPε isoform, with loss of the dimerization domain, DNA binding region, and transcriptional activity. (rupress.org)
- N-terminal nonsense mutations result in a dominant negative C/EBP-alpha protein while C-terminal mutations reduce the DNA-binding potential of this transcription factor. (genecards.org)
- Additionally, sequence variation at C/EBP site I, which lies immediately upstream of the distal nuclear factor kappa B site and immediately downstream of a binding site for activating transcription factor (ATF)/cyclic AMP response element binding protein (CREB), has been shown to affect HIV-1 long terminal repeat (LTR) activity. (asm.org)
- Transcriptional activators include C/EBP α ( 4 ), C/EBP β (nuclear factor interleukin-6), (IL-6) ( 2 , 9 , 12 , 40 ), C/EBP δ ( 42 ), C/EBP ɛ ( 44 ), and C/EBP-related protein 1 (CRP-1) ( 42 ). (asm.org)
- E2F acts as a co-repressor of another transcription factor, C/EBPa, without binding to cis-regulatory elements, but by direct protein-protein interactions that abolish the binding of C/EBPa to DNA. (hu-berlin.de)
- This work demonstrates an association of C/EBPa to the RNA Pol I transcription factor UBF1, both proteins retained in large chromosomal foci. (hu-berlin.de)
- We discovered that the accumulation of fetal-like hepatocytes in injured livers is controlled by injury-related activation of Yes-associated protein-1 and Insulin-like growth factor-2 RNA-binding protein-3, two fetal factors that are normally silenced in healthy adult hepatocytes. (bmj.com)
- Tumour necrosis factor-alpha inhibits adipogenesis via a beta-catenin/TCF4(TCF7L2)-dependent pathway. (nih.gov)
- The CCAAT enhancer-binding protein alpha (C/EBPalpha) requires a SWI/SNF complex for proliferation arrest. (wikipedia.org)
- Deoxyribonuclease (DNase) I footprinting analysis revealed that 5′ flanking region (−232 to −210 bp) is acute-phase response protein-binding site. (ahajournals.org)
- Atherosclerosis is accelerated by acute-phase response, which involves altered hepatic protein synthesis. (ahajournals.org)
- 1 Plasminogen activator inhibitor-1 (PAI-1), the physiological inhibitor of fibrinolysis, is an acute-phase protein that impairs fibrinolysis. (ahajournals.org)
- 4-9 Increases of acute-phase proteins including PAI-1 are attributable largely to changes in hepatic productions by proinflammatory cytokines. (ahajournals.org)
- Interleukin-6 (IL-6) is the principal stimulator of most acute-phase proteins. (ahajournals.org)
- FOXA3 (Forkhead Box A3) is a Protein Coding gene. (genecards.org)
- and looping the PXR-bound distal phenobarbital-responsive enhancer module toward the proximal C/EBP binding site. (aspetjournals.org)
- C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. (creativebiomart.net)
- C/EBP proteins also contain activation domains at the N-terminus and regulatory domains. (wikipedia.org)
- A variant of signal regulatory protein alpha (SIRPa) that antagonizes the human cell surface antigen CD47, with potential phagocytosis-inducing, immunostimulating and antineoplastic activities. (cancer.gov)
- C/EBPβ can interact with CREB, NF-κB, and other proteins, leading to a trans-activation potential. (wikipedia.org)
- It has been shown that natural PPARγ ligands, such as prostaglandins ( 11 ), fatty acids ( 12 ), or synthetic ligands such as thiazolidinediones (TZDs), bind to PPARγ and lead to ligand-dependent activation. (diabetesjournals.org)
- FoxO1 and FoxO3a (collectively FoxO1/3a) proteins regulate a wide array of cellular processes, including hepatic gluconeogenesis. (diabetesjournals.org)
- also known as protein arginine methyltransferase 4 (PRMT4)] is one of nine members of the protein arginine methyltransferase (PRMT) family that regulate crucial cellular functions, including transcription, mRNA processing and stability, and translation. (biologists.org)
- 12-14 Furthermore, recent evidence suggests that 14-3-3 proteins modulate crucial aspects of heart function both in vitro and in vivo. (ahajournals.org)
- First, low amounts of CREB-1 and C/EBP appear to heterodimerize and bind to a site consisting of a half site from both the ATF/CREB and C/EBP binding sites. (asm.org)
- This interaction is reciprocal, since C/EBP dimer binding to a strong C/EBP site leads to enhanced CREB-1 recruitment to ATF/CREB sites that are weakly bound by CREB. (asm.org)
- The other type of mutation blocks the DNA-binding ability of CCAAT enhancer-binding protein alpha. (nih.gov)
- The bar plot below shows the proportion of tumor samples that have any kind of altering mutation(s) in the given protein. (phosphosite.org)
- These data also inform how each germline mutation uniquely disrupts protein function(s), whether it generates haploinsufficiency, dominant-negative or ectopic protein functions, or amalgamated gain-of-function and loss-of-function defects to disrupt cellular phenotypes and instigate MDS/AML. (jci.org)