Ccaat enhancer binding protein alpha. Medical search

A CCAAT-enhancer-binding protein found in LIVER; ADIPOSE TISSUE; INTESTINES; LUNG; ADRENAL GLANDS; PLACENTA; OVARY and peripheral blood mononuclear cells (LEUKOCYTES, MONONUCLEAR). Experiments with knock-out mice have demonstrated that CCAAT-enhancer binding protein-alpha is essential for the functioning and differentiation of HEPATOCYTES and ADIPOCYTES.

A class of proteins that were originally identified by their ability to bind the DNA sequence CCAAT. The typical CCAAT-enhancer binding protein forms dimers and consists of an activation domain, a DNA-binding basic region, and a leucine-rich dimerization domain (LEUCINE ZIPPERS). CCAAT-BINDING FACTOR is structurally distinct type of CCAAT-enhancer binding protein consisting of a trimer of three different subunits.

A CCAAT-enhancer-binding protein found in LIVER; INTESTINES; LUNG and ADIPOSE TISSUE. It is an important mediator of INTERLEUKIN-6 signaling.

A member of the C-EBP protein family of transcription factors. It plays a key role in G0 PHASE mammary EPITHELIAL CELL growth arrest, and it is involved in transcriptional regulation of INTERLEUKIN 1; INTERLEUKIN 6; and TUMOR NECROSIS FACTOR-ALPHA.

Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.

Cells in the body that store FATS, usually in the form of TRIGLYCERIDES. WHITE ADIPOCYTES are the predominant type and found mostly in the abdominal cavity and subcutaneous tissue. BROWN ADIPOCYTES are thermogenic cells that can be found in newborns of some species and hibernating mammals.

DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.

Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.

Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.

The differentiation of pre-adipocytes into mature ADIPOCYTES.

Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.

Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.

A continuous cell line that is a substrain of SWISS 3T3 CELLS developed though clonal isolation. The mouse fibroblast cells undergo an adipose-like conversion as they move to a confluent and contact-inhibited state.

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.

Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.

Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.

Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.

RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.

A nuclear transcription factor. Heterodimerization with RETINOID X RECEPTOR ALPHA is important in regulation of GLUCOSE metabolism and CELL GROWTH PROCESSES. It is a target of THIAZOLIDINEDIONES for control of DIABETES MELLITUS.

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in leukemia.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.

The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.

Established cell cultures that have the potential to propagate indefinitely.

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

A CCAAT-enhancer binding protein that is induced by DNA DAMAGE and growth arrest. It serves as a dominant negative inhibitor of other CCAAT-enhancer binding proteins.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.

An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.

The parts of a macromolecule that directly participate in its specific combination with another molecule.

A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.

Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. These elements may be found in both promoter and enhancer regions.

A family of DNA binding proteins that regulate expression of a variety of GENES during CELL DIFFERENTIATION and APOPTOSIS. Family members contain a highly conserved carboxy-terminal basic HELIX-TURN-HELIX MOTIF involved in dimerization and sequence-specific DNA binding.

The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.

Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)

Formation of MYELOID CELLS from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW via MYELOID STEM CELLS. Myelopoiesis generally refers to the production of leukocytes in blood, such as MONOCYTES and GRANULOCYTES. This process also produces precursor cells for MACROPHAGE and DENDRITIC CELLS found in the lymphoid tissue.

The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.

The GTPase-containing subunits of heterotrimeric GTP-binding proteins. When dissociated from the heterotrimeric complex these subunits interact with a variety of second messenger systems. Hydrolysis of GTP by the inherent GTPase activity of the subunit causes it to revert to its inactive (heterotrimeric) form. The GTP-Binding protein alpha subunits are grouped into families according to the type of action they have on second messenger systems.

Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.

A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).

Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.

A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.

Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.

Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.

A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.

Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.

Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.

Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.

DNA-binding motifs formed from two alpha-helixes which intertwine for about eight turns into a coiled coil and then bifurcate to form Y shaped structures. Leucines occurring in heptad repeats end up on the same sides of the helixes and are adjacent to each other in the stem of the Y (the "zipper" region). The DNA-binding residues are located in the bifurcated region of the Y.

Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.

An organochlorine compound that was formerly used as an insecticide. Its manufacture and use has been discontinued in the United States. (From Merck Index, 11th ed)

Nucleic acid sequences involved in regulating the expression of genes.

A forkhead transcription factor that regulates expression of metabolic GENES and is involved in EMBRYONIC DEVELOPMENT. Mutations in HNF-3beta have been associated with CONGENITAL HYPERINSULINISM.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.

A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.

An early local inflammatory reaction to insult or injury that consists of fever, an increase in inflammatory humoral factors, and an increased synthesis by hepatocytes of a number of proteins or glycoproteins usually found in the plasma.

A component of NF-kappa B transcription factor. It is proteolytically processed from NF-kappa B p105 precursor protein and is capable of forming dimeric complexes with itself or with TRANSCRIPTION FACTOR RELA. It regulates expression of GENES involved in immune and inflammatory responses.

Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.

A human cell line established from a diffuse histiocytic lymphoma (HISTIOCYTIC LYMPHOMA, DIFFUSE) and displaying many monocytic characteristics. It serves as an in vitro model for MONOCYTE and MACROPHAGE differentiation.

Transport proteins that carry specific substances in the blood or across cell membranes.

A cell line derived from cultured tumor cells.

An anti-inflammatory 9-fluoro-glucocorticoid.

A DNA-directed RNA polymerase found in BACTERIA. It is a holoenzyme that consists of multiple subunits including sigma factor 54.

A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. This protein functions to integrate both calcium and cAMP signals.

Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.

The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.

Intracellular receptors that can be found in the cytoplasm or in the nucleus. They bind to extracellular signaling molecules that migrate through or are transported across the CELL MEMBRANE. Many members of this class of receptors occur in the cytoplasm and are transported to the CELL NUCLEUS upon ligand-binding where they signal via DNA-binding and transcription regulation. Also included in this category are receptors found on INTRACELLULAR MEMBRANES that act via mechanisms similar to CELL SURFACE RECEPTORS.

Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA.

Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.

A group of CORTICOSTEROIDS that affect carbohydrate metabolism (GLUCONEOGENESIS, liver glycogen deposition, elevation of BLOOD SUGAR), inhibit ADRENOCORTICOTROPIC HORMONE secretion, and possess pronounced anti-inflammatory activity. They also play a role in fat and protein metabolism, maintenance of arterial blood pressure, alteration of the connective tissue response to injury, reduction in the number of circulating lymphocytes, and functioning of the central nervous system.

Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)

Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.

Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.

A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.

A multiprotein complex composed of the products of c-jun and c-fos proto-oncogenes. These proteins must dimerize in order to bind to the AP-1 recognition site, also known as the TPA-responsive element (TRE). AP-1 controls both basal and inducible transcription of several genes.

The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.

Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.

Various physiological or molecular disturbances that impair ENDOPLASMIC RETICULUM function. It triggers many responses, including UNFOLDED PROTEIN RESPONSE, which may lead to APOPTOSIS; and AUTOPHAGY.

Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.

A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.

Histochemical localization of immunoreactive substances using labeled antibodies as reagents.

Parent cells in the lineage that gives rise to MONOCYTES and MACROPHAGES.

One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.

The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

All of the processes involved in increasing CELL NUMBER including CELL DIVISION.

Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.

Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.

Proteins prepared by recombinant DNA technology.

A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)

The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.

Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.

Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)

A primary malignant neoplasm of epithelial liver cells. It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. The cells may be uniform or markedly pleomorphic, or form GIANT CELLS. Several classification schemes have been suggested.

A large group of membrane transport proteins that shuttle MONOSACCHARIDES across CELL MEMBRANES.

The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.

The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.

A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.

The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Proteins that bind to the 3' polyadenylated region of MRNA. When complexed with RNA the proteins serve an array of functions such as stabilizing the 3' end of RNA, promoting poly(A) synthesis and stimulating mRNA translation.

An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. This catalyzes endonucleolytic cleavage of DNA yielding 5'-phosphodi- and oligonucleotide end-products. The enzyme has a preference for double-stranded DNA.

Intracellular proteins that reversibly bind hydrophobic ligands including: saturated and unsaturated FATTY ACIDS; EICOSANOIDS; and RETINOIDS. They are considered a highly conserved and ubiquitously expressed family of proteins that may play a role in the metabolism of LIPIDS.

Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.

A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.

Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.

Thapsigargin suppresses phorbol ester-dependent human involucrin promoter activity by suppressing CCAAT-enhancer-binding protein alpha (C/EBPalpha) DNA binding. (1/560)

Human involucrin (hINV) is a keratinocyte differentiation marker expressed in the suprabasal epidermal layers. In cultured keratinocytes hINV mRNA levels are increased 10-fold by a 24-h treatment with 50 ng/ml PMA, an agent that promotes keratinocyte differentiation. Previous studies show that thapsigargin (TGN), an agent that depletes intracellular calcium stores, inhibits keratinocyte differentiation. In the present study we show that TGN inhibits the PMA-dependent, differentiation-associated, increase in hINV mRNA levels and hINV promoter activity. Inhibition is half-maximal at 10 nM and maximal at 100 nM TGN. Neither basal hINV promoter activity nor glyceraldehyde-3-phosphate dehydrogenase mRNA levels are inhibited. Mutation of a functionally important CAATT-enhancer-binding protein (C/EBP) site within the hINV promoter proximal regulatory region eliminates the regulation, suggesting that TGN may effect C/EBP-dependent promoter activation. Consistent with this hypothesis, TGN inhibits C/EBPalpha-dependent promoter activation via a mechanism that involves inhibition of C/EBPalpha binding to DNA without changing C/EBPalpha protein levels. These results suggest that TGN interferes with hINV expression by interfering with C/EBP transcription-factor function. (+info)

Identification and characterization of leptin-containing intracellular compartment in rat adipose cells. (2/560)

The major leptin-containing membrane compartment was identified and characterized in rat adipose cells by means of equilibrium density and velocity sucrose gradient centrifugation. This compartment appears to be different from peptide-containing secretory granules present in neuronal, endocrine, and exocrine cells, as well as from insulin-sensitive GLUT-4-containing vesicles abundant in adipocytes. Exocytosis of both leptin- and GLUT-4-containing vesicles can be induced by insulin; however, only leptin secretion is responsive to serum stimulation. This latter effect is resistant to cycloheximide, suggesting that serum triggers the release of a stored pool of presynthesized leptin molecules. We conclude that regulated secretion of leptin and insulin-dependent translocation of GLUT-4 represent different pathways of membrane trafficking in rat adipose cells. NIH 3T3 cells ectopically expressing CAAT box enhancer binding protein-alpha and Swiss 3T3 cells expressing peroxisome proliferator-activated receptor-gamma undergo differentiation in vitro and acquire adipocyte morphology and insulin-responsive glucose uptake. Only the former cell line, however, is capable of leptin secretion. Thus different transcriptional mechanisms control the developmental onset of these two major and independent physiological functions in adipose cells. (+info)

Role of C/EBP homologous protein (CHOP-10) in the programmed activation of CCAAT/enhancer-binding protein-beta during adipogenesis. (3/560)

Hormone induction of growth-arrested preadipocytes triggers mitotic clonal expansion followed by expression of CCAAT/enhancer-binding protein (C/EBP)alpha and differentiation into adipocytes. The order of these events is critical because C/EBPalpha is antimitotic and its expression prematurely would block the mitotic clonal expansion required for differentiation. C/EBPbeta, a transcriptional activator of the C/EBPalpha gene, is expressed early in the differentiation program, but lacks DNA-binding activity and fails to localize to centromeres until preadipocytes traverse the G(1)-S checkpoint of mitotic clonal expansion. Evidence is presented that dominant-negative CHOP-10 expressed by growth-arrested preadipocytes transiently sequesters C/EBPbeta by heterodimerization. As preadipocytes reach S phase, CHOP-10 is down-regulated, apparently releasing C/EBPbeta from inhibitory constraint and allowing transactivation of the C/EBPalpha gene. In support of these findings, up-regulation of CHOP-10 with the protease inhibitor N-acetyl-Leu-Leu-norleucinal prevents activation of C/EBPbeta, expression of C/EBPalpha, and adipogenesis. (+info)

Sequential repression and activation of the CCAAT enhancer-binding protein-alpha (C/EBPalpha ) gene during adipogenesis. (4/560)

CCAAT enhancer-binding protein-alpha (C/EBPalpha) functions as a pleiotropic transcriptional activator of adipocyte genes during adipogenesis. Nuclear factor C/EBP undifferentiated protein (CUP), an isoform of activator protein-2alpha (AP-2alpha), binds to repressive elements in the C/EBPalpha gene promoter, silencing the gene until late in the differentiation program. The CUP regulatory element overlaps a Sp (GT-box) element in the promoter to which Sp3 (or Sp1) can bind. Binding by Sp3 or Sp1 and CUP/AP2-alpha is mutually exclusive. Sp3 is a strong transcriptional activator of the C/EBPalpha gene promoter in 3T3-L1 preadipocytes and Schneider cells, this activation being repressed by CUP/AP-2alpha. Sp3 is expressed throughout differentiation, whereas CUP/AP-2alpha, which is expressed only by preadipocytes, is down-regulated during differentiation coincident with transcription of the C/EBPalpha gene. Thus, CUP/AP-2alpha delays access of Sp3 to the Sp regulatory element, preventing premature expression of C/EBPalpha and thereby interference by C/EBPalpha (which is antimitotic) with mitotic clonal expansion, an essential early event in the differentiation program. (+info)

CREB (cAMP response element binding protein) and C/EBPalpha (CCAAT/enhancer binding protein) are required for the superstimulation of phosphoenolpyruvate carboxykinase gene transcription by adenoviral E1a and cAMP. (5/560)

In the present study, we observed superstimulated levels of cAMP-stimulated transcription from the phosphoenolpyruvate carboxykinase (PEPCK) gene promoter in cells infected with wild-type adenovirus expressing 12 S and 13 S E1a proteins, or in cells expressing 13 S E1a alone. cAMP-stimulated transcription was inhibited in cells expressing only 12 S E1a, but slightly elevated in cells expressing E1a proteins with mutations in conserved regions 1 or 2, leading us to conclude that the superstimulation was mediated by conserved region 3 of 13 S E1a. E1a failed to enhance cAMP-stimulated transcription from promoters containing mutations that abolish binding by cAMP response element binding protein (CREB) or CCAAT/enhancer binding proteins (C/EBPs). This result was supported by experiments in which expression of dominant-negative CREB and/or C/EBP proteins repressed E1a- and cAMP-stimulated transcription from the PEPCK gene promoter. In reconstitution experiments using a Gal4-responsive promoter, E1a enhanced cAMP-stimulated transcription when chimaeric Gal4-CREB and Gal4-C/EBPalpha were co-expressed. Phosphorylation of CREB on serine-133 was stimulated in cells treated with dibutyryl cAMP, whereas phosphorylation of C/EBPalpha was increased by E1a expression. Our data support a model in which cAMP agonists increase CREB activity and stimulate PEPCK gene transcription, a process that is enhanced by E1a through the phosphorylation of C/EBPalpha. (+info)

The rat ortholog of the presumptive flounder antifreeze enhancer-binding protein is a helicase domain-containing protein. (6/560)

The expression of winter flounder liver-type antifreeze protein (wflAFP) genes is tissue-specific and under seasonal and hormonal regulation. The only intron of the major wflAFP gene was demonstrated to be a liver-specific enhancer in both mammalian cell lines and flounder hepatocytes. Element B, the core enhancer sequence, was shown to interact specifically with a liver-enriched transcription factor, CCAAT/enhancer-binding protein alpha (C/EBPalpha), as well as a presumptive antifreeze enhancer-binding protein (AEP). In this study, the identity of the rat AEP ortholog was revealed via its DNA-protein interaction with element B. It is a helicase-domain-containing protein, 988 amino acids in length, and is homologous to mouse Smubp-2, hamster Rip-1 and human Smubp-2. The specific binding between element B and AEP was confirmed by South-Western analysis and gel retardation assays. Residues in element B important to this interaction were identified by methylation interference assays. Mutation on one of the residues disrupted the binding between element B and AEP and its enhancer activity was significantly reduced, suggesting that AEP is essential for the transactivation of the wflAFP gene intron. The rat AEP is ubiquitously expressed in various tissues, and the flounder homolog is present as shown by genomic Southern analysis. The potential role of AEP in regulating the flounder AFP gene expression is discussed. (+info)

Interferon-gamma-induced regulation of peroxisome proliferator-activated receptor gamma and STATs in adipocytes. (7/560)

Interferon-gamma (IFN-gamma) is known primarily for its roles in immunological responses but also has been shown to affect fat metabolism and adipocyte gene expression. To further investigate the effects of IFN-gamma on fat cells, we examined the effects of this cytokine on the expression of adipocyte transcription factors in 3T3-L1 adipocytes. Although IFN-gamma regulated the expression of several adipocyte transcription factors, IFN-gamma treatment resulted in a rapid reduction of both peroxisome proliferator-activated receptor (PPAR) protein and mRNA. A 48-h exposure to IFN-gamma also resulted in a decrease of both CCAAT/enhancer-binding alpha and sterol regulatory element binding protein (SREBP-1) expression. The short half-life of both the PPARgamma mRNA and protein likely contributed to the rapid decline of both cytosolic and nuclear PPARgamma in the presence of IFN-gamma. Our studies clearly demonstrated that the IFN-gamma-induced loss of PPARgamma protein is partially inhibited in the presence of two distinct proteasome inhibitors. Moreover, IFN-gamma also inhibited the transcription of PPARgamma, which was accompanied by a decrease in PPARgamma mRNA accumulation. In addition, exposure to IFN-gamma resulted in a substantial increase in STAT 1 expression and a small increase in STAT 3 expression. IFN-gamma treatment of 3T3-L1 adipocytes (48-96 h) resulted in a substantial inhibition of insulin-sensitive glucose uptake. These data clearly demonstrate that IFN-gamma treatment results in the development of insulin resistance, which is accompanied by the regulation of various adipocyte transcription factors, in particular the synthesis and degradation of PPARgamma. (+info)

Hormonal signaling and transcriptional control of adipocyte differentiation. (8/560)

Recent advances regarding the biology of adipose tissue have identified the adipocyte as an important mediator in many physiologic and pathologic processes regarding energy metabolism. Consideration for a central role of adipose tissue in the development of obesity, cardiovascular disease and noninsulin-dependent diabetes mellitus has resulted in new incentives toward understanding the complexities of adipocyte differentiation. Current knowledge of this process includes a cascade of transcriptional events that culminate in the expression of peroxisome proliferator-activated receptor-gamma (PPARgamma) and CCAAT/enhancer binding protein-alpha (C/EBPalpha). These prominent adipogenic transcription factors have been shown to regulate, directly or indirectly, the gene expression necessary for the development of the mature adipocyte. Hormonal and nutritional signaling that impinges on these trans-acting factors provides a molecular link between lipids and lipid-related compounds and the gene expression important for glucose and lipid homeostasis. Knowledge concerning the transcriptional events mediating adipocyte differentiation provides a basis for understanding the physiologic processes associated with adipose tissue as well as for the development of therapeutic interventions in obesity and its related disorders. (+info)

CCAAT/enhancer-binding protein alpha is a protein encoded by the CEBPA gene in humans. CCAAT/enhancer-binding protein alpha is ... One category of mutations prevent CCAAT/enhancer-binding protein alpha DNA binding by altering its COOH-terminal basic leucine ... For details on the CCAAT structural motif in gene enhancers and on CCAAT/Enhancer Binding Proteins see the specific page. The ... "Entrez Gene: CEBPA CCAAT/enhancer binding protein (C/EBP), alpha". Ohlsson E, Schuster MB, Hasemann M, Porse BT (Apr 2016). " ...

https://en.wikipedia.org/wiki/CEBPA

Freytag SO, Paielli DL, Gilbert JD (Jul 1994). "Ectopic expression of the CCAAT/enhancer-binding protein alpha promotes the ... "CCAAT/enhancer-binding protein family members recruit the coactivator CREB-binding protein and trigger its phosphorylation". ... CCAAT-enhancer-binding proteins (or C/EBPs) is a family of transcription factors composed of six members, named from C/EBPα to ... CCAAT/enhancer-binding proteins are often involved in growth arrest and differentiation, which has been interpreted to suggest ...

https://en.wikipedia.org/wiki/CCAAT-enhancer-binding_proteins

"Different regulation of the LXRalpha promoter activity by isoforms of CCAAT/enhancer-binding proteins". Biochemical and ... Liver X receptor alpha (LXR-alpha) is a nuclear receptor protein that in humans is encoded by the NR1H3 gene (nuclear receptor ... "The atypical interaction of peroxisome proliferator-activated receptor alpha with liver X receptor alpha antagonizes the ... peroxisome proliferator-activated receptor alpha and liver-X-receptor alpha". Molecular and Cellular Endocrinology. 146 (1-2): ...

https://en.wikipedia.org/wiki/Liver_X_receptor_alpha

"Tumor Necrosis Factor Alpha Inhibits Type I Collagen Synthesis through Repressive CCAAT/Enhancer-Binding Proteins". Mol. Cell. ... CCAAT/enhancer-binding protein beta is a protein that in humans is encoded by the CEBPB gene. The protein encoded by this ... "Entrez Gene: CEBPB CCAAT/enhancer binding protein (C/EBP), beta". Ruffell D, Mourkioti F, Gambardella A, Kirstetter P, Lopez RG ... Chen GK, Sale S, Tan T, Ermoian RP, Sikic BI (April 2004). "CCAAT/enhancer-binding protein beta (nuclear factor for interleukin ...

https://en.wikipedia.org/wiki/CEBPB

Boruk M, Savory JG, Haché RJ (November 1998). "AF-2-dependent potentiation of CCAAT enhancer binding protein beta-mediated ... March 2001). "A subfamily of RNA-binding DEAD-box proteins acts as an estrogen receptor alpha coactivator through the N- ... "Entrez Gene: DBI diazepam binding inhibitor (GABA receptor modulator, acyl-Coenzyme A binding protein)". Kos M, Reid G, Denger ... March 2000). "Tamoxifen-bound estrogen receptor (ER) strongly interacts with the nuclear matrix protein HET/SAF-B, a novel ...

https://en.wikipedia.org/wiki/Estrogen_receptor_alpha

CCAAT/enhancer-binding protein delta is a protein that in humans is encoded by the CEBPD gene. The protein encoded by this ... It can also form heterodimers with the related protein CEBP-alpha. The encoded protein is important in the regulation of genes ... "CCAAT/enhancer-binding protein family members recruit the coactivator CREB-binding protein and trigger its phosphorylation". ... "Entrez Gene: CEBPD CCAAT/enhancer binding protein (C/EBP), delta". Gery S, Tanosaki S, Hofmann WK, Koppel A, Koeffler HP (Feb ...

https://en.wikipedia.org/wiki/CEBPD

March 2001). "Dominant-negative mutations of CEBPA, encoding CCAAT/enhancer binding protein-alpha (C/EBPalpha), in acute ... an aggregate of multiple copies of the same protein, otherwise known as a homomultimeric protein or homooligomeric protein. In ... For example, in humans the Hb gene locus is responsible for the Beta-chain protein (HBB) that is one of the two globin proteins ... A mutation that leads to a mutant protein that disrupts the activity of the wild-type protein in the multimer is a dominant- ...

https://en.wikipedia.org/wiki/Dominance_(genetics)

Jurado LA, Song S, Roesler WJ, Park EA (2002). "Conserved amino acids within CCAAT enhancer-binding proteins (C/EBP(alpha) and ... A mitochondrial isozyme of the encoded protein also has been characterized. Click on genes, proteins and metabolites below to ... Wilson HL, McFie PJ, Roesler WJ (2003). "Different transcription factor binding arrays modulate the cAMP responsivity of the ... 2002). "Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site". J. Mol. Biol. ...

https://en.wikipedia.org/wiki/PCK1

"Transcriptional regulation of human CYP3A4 basal expression by CCAAT enhancer-binding protein alpha and hepatocyte nuclear ... "Cloning and characterization of seven human forkhead proteins: binding site specificity and DNA bending". The EMBO Journal. 13 ... Forkhead box I1 is a protein that in humans is encoded by the FOXI1 gene. This gene belongs to the forkhead family of ... FOX proteins GRCh38: Ensembl release 89: ENSG00000168269 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000047861 - ...

https://en.wikipedia.org/wiki/FOXI1

2003). "Transcriptional regulation of human CYP3A4 basal expression by CCAAT enhancer-binding protein alpha and hepatocyte ... is a protein that in humans is encoded by the FOXA3 gene. HNF-3G is a member of the forkhead class of DNA-binding proteins. ... 2009). "Differential binding and co-binding pattern of FOXA1 and FOXA3 and their relation to H3K4me3 in HepG2 cells revealed by ... Hepatocyte nuclear factor 3-gamma (HNF-3G), also known as forkhead box protein A3 (FOXA3) or transcription factor 3G (TCF-3G) ...

https://en.wikipedia.org/wiki/FOXA3

... of a co-repressor that inhibits the transcriptional and growth-arrest activities of CCAAT/enhancer-binding protein alpha". J. ... 2000). "Protein-interaction modules that organize nuclear function: FF domains of CA150 bind the phosphoCTD of RNA polymerase ... 2004). "A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease". Mol. Cell. ... This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein ...

https://en.wikipedia.org/wiki/Transcription_elongation_regulator_1

CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. Ccaat-enhancer-binding proteins ... 1992). "Molecular cloning of two C/EBP-related proteins that bind to the promoter and the enhancer of the alpha 1-fetoprotein ... CCAAT/enhancer-binding protein gamma is a protein that in humans is encoded by the CEBPG gene. The C/EBP family of ... "Entrez Gene: CEBPG CCAAT/enhancer binding protein (C/EBP), gamma". Nishizawa M, Nagata S (1992). "cDNA clones encoding leucine- ...

https://en.wikipedia.org/wiki/CEBPG

... studies indicate a role for CCAAT enhancer binding proteins alpha and epsilon (C/EBP alpha and C/EBP epsilon ) and CDP/cut in ... CCAAT/enhancer binding protein (C/EBP), epsilon, also known as CEBPE and CRP1, is a type of ccaat-enhancer-binding protein. ... "Entrez Gene: CEBPE CCAAT/enhancer binding protein (C/EBP), epsilon". Antonson P, Stellan B, Yamanaka R, Xanthopoulos KG (Jul ... Antonson P, Stellan B, Yamanaka R, Xanthopoulos KG (Jul 1996). "A novel human CCAAT/enhancer binding protein gene, C/EBPepsilon ...

https://en.wikipedia.org/wiki/CEBPE

"A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy ... E3 SUMO-protein ligase PIAS4 is one of several protein inhibitor of activated STAT (PIAS) proteins. It is also known as protein ... Mothers against decapentaplegic homolog 7 and Lymphoid enhancer-binding factor 1. GRCh38: Ensembl release 89: ENSG00000105229 ... "Entrez Gene: PIAS4 Protein inhibitor of activated STAT, 4". Imoto, Seiyu; Sugiyama Kenji; Muromoto Ryuta; Sato Noriko; Yamamoto ...

https://en.wikipedia.org/wiki/PIAS4

"A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy ... Eaton EM, Sealy L (August 2003). "Modification of CCAAT/enhancer-binding protein-beta by the small ubiquitin-like modifier ( ... Dobreva G, Dambacher J, Grosschedl R (December 2003). "SUMO modification of a novel MAR-binding protein, SATB2, modulates ... Small ubiquitin-related modifier 3 is a protein that in humans is encoded by the SUMO3 gene. SUMO proteins, such as SUMO3, and ...

https://en.wikipedia.org/wiki/SUMO3

Zuo Y, Qiang L, Farmer SR (March 2006). "Activation of CCAAT/enhancer-binding protein (C/EBP) alpha expression by C/EBP beta ... inhibited adipogenesis and repressed induction of the master regulators PPARγ and CCAAT/enhancer-binding protein alpha (CEBPA ... binding protein], PACT (protein activator of the interferon-induced protein kinase), the SMN complex, fragile X mental ... These proteins have three highly positively charged regions, termed AT hooks, that bind the minor groove of AT-rich DNA ...

https://en.wikipedia.org/wiki/MicroRNA

CCAAT/enhancer-binding protein alpha), glucocorticoid receptors α and β, and p53. Expression of isoforms 1 and 2 has been ... these tyrosine motifs are bound by the SH2 domains of signaling proteins. Important binding partners for this region include ... allowing the CAS protein to function as a scaffold for other proteins including CRK proteins and C3G, a guanine nucleotide ... All CAS proteins except CASS4 contain a YDYVHL motif within this domain, which is an important binding site for the Src SH2 ...

https://en.wikipedia.org/wiki/Embryonal_fyn-associated_substrate

... found in inflammatory zone 1/resistin-like molecule alpha gene expression by a STAT6 and CCAAT/enhancer-binding protein- ... 2003). "The Secreted Protein Discovery Initiative (SPDI), a Large-Scale Effort to Identify Novel Human Secreted and ... Resistin-like beta is a protein that in humans is encoded by the RETNLB gene. GRCh38: Ensembl release 89: ENSG00000163515 - ... 2003). "Bacterial colonization leads to the colonic secretion of RELMbeta/FIZZ2, a novel goblet cell-specific protein". ...

https://en.wikipedia.org/wiki/RETNLB

CCAAT/enhancer-binding protein alpha, liver activator protein, and D-element-binding protein". Mol. Cell. Biol. 12 (7): 3023-31 ... interaction of CCAAT/enhancer-binding protein with elements flanking the ADH2 TATA box". Gene. 90 (2): 271-9. doi:10.1016/0378- ... 1986). "cDNA and protein structure for the alpha subunit of human liver alcohol dehydrogenase". Biochemistry. 25 (9): 2465-70. ... Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. This gene is ...

https://en.wikipedia.org/wiki/ADH1A

... activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the ... "Extracellular HIV-1 Tat protein induces a rapid and selective activation of protein kinase C (PKC)-alpha, and -epsilon and - ... Protein kinase C eta type is an enzyme that in humans is encoded by the PRKCH gene. Protein kinase C (PKC) is a family of ... "Entrez Gene: PRKCH protein kinase C, eta". Greif H, Ben-Chaim J, Shimon T, et al. (1992). "The protein kinase C-related PKC-L( ...

https://en.wikipedia.org/wiki/PRKCH

CCAAT binding factors, or CCAAT enhancer binding proteins were found. The TMEM251 protein is 169 amino acids in length. The ... 71.6% alpha helix. 8.9% turns It is predicted to have two transmembrane helices, of 23 amino acids in length each. The average ... Transmembrane protein 251, also known as C14orf109 or UPF0694, is a protein that in humans is encoded by the TMEM251 gene. One ... No vertebrate TATA binding protein factors, RNA polymerase transcription factor II B, ...

https://en.wikipedia.org/wiki/Transmembrane_protein_251

Transcription factors, peroxis proliferator-activated receptor γ (PPARγ) and CCAAT enhancer-binding proteins (C/EBPs) are main ... February 1999). "Cross-regulation of C/EBP alpha and PPAR gamma controls the transcriptional pathway of adipogenesis and ... cAMP-responsive element binding protein promotes differentiation, while the activation of PPARγ and C/EBPα is also responsive ... T-cell factor/lymphoid enhancer-binding factor (TCF/LEF), GATA2/3, retinoic acid receptor α, and SMAD6/7 don't affect the ...

https://en.wikipedia.org/wiki/Adipogenesis

... by activating a p38 delta mitogen-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha". J. Biol ... "Extracellular HIV-1 Tat protein induces a rapid and selective activation of protein kinase C (PKC)-alpha, and -epsilon and - ... Serine/threonine-protein kinase D3 (PKD3) or PKC-nu is an enzyme that in humans is encoded by the PRKD3 gene. Protein kinase C ... human immunodeficiency virus type 1 Tat protein is associated with an increase in both NF-kappa B binding and protein kinase C ...

https://en.wikipedia.org/wiki/PRKD3

... forkhead box protein A1 (FoxA1), and CCAAT enhancer-binding protein b (C/EBPb)] of FTMT transcription have been identified ( ... "Sp1 and Smad proteins cooperate to mediate transforming growth factor-beta 1-induced alpha 2(I) collagen expression in human ... "Functional cooperation of simian virus 40 promoter factor 1 and CCAAT/enhancer-binding protein beta and delta in ... Sp1 and cAMP-response-element-binding protein-binding protein (CBP/p300)". The Biochemical Journal. 339 (3): 751-8. doi:10.1042 ...

https://en.wikipedia.org/wiki/Sp1_transcription_factor

"CCAAT/Enhancer-binding Protein Family Members Recruit the Coactivator CREB-binding Protein and Trigger Its Phosphorylation". ... The alpha helix segments span from amino acids 2-9, 21-24, 47-58, 61-94, and 104-111 in the protein sequence. ERICH4 is not ... "CCAAT/enhancer-binding proteins: structure, function and regulation". Biochemical Journal. 365 (3): 561-575. doi:10.1042/ ... As suggested by the protein's name, glutamate-rich protein 4, the protein is most highly composed of glutamic acid amino acids ...

https://en.wikipedia.org/wiki/Glutamate-rich_protein_4

Stimulating protein 1, CCAAT/enhancer binding protein, GC box elements and HMG box-containing protein 1. Like previously ... PANO1 appears to diverge much more quickly than fibrinogen alpha and much more quickly than cytochrome c. p14ARF is a protein ... "Protein BLAST: search protein databases using a protein query". blast.ncbi.nlm.nih.gov. Retrieved 2021-08-01. (CS1 maint: url- ... PANO1 is a protein which in humans is encoded by the PANO1 gene. PANO1 is an apoptosis inducing protein that is able to ...

https://en.wikipedia.org/wiki/PANO1

Ccaat/Enhancer binding protein (CEBP) cAMP-responsive element binding proteins (CREB) Vertebrate TATA binding protein factor ( ... two alpha-helical segments were predicted. Predictive models made by Phyre2 and SWISS-Model have shown two alpha-helical ... "Protein BLAST: search protein databases using a protein query". blast.ncbi.nlm.nih.gov. Retrieved 2020-03-02. "Genomatix - NGS ... C/EBP homologous protein (CHOP) X-box binding factors (XBBF) TALE homeodomain class recognizing TG motifs (TALE) Human and ...

https://en.wikipedia.org/wiki/C5orf46

Transcription factor assessment indicates many potential TATA-binding protein and CCAAT-enhancer-binding proteins sites, along ... C1orf112 secondary structure is predicted to be predominately alpha helical, with < 5% of the protein composed of beta sheets. ... C1orf112 is predicted to interact with a diverse range of proteins, including multiple mitosis-associated proteins. C1orf112 is ... quality-controlled protein-protein association networks, made broadly accessible". Nucleic Acids Research. 45 (D1): D362-D368. ...

https://en.wikipedia.org/wiki/C1orf112

Ccaat-enhancer binding protein KRAB domain zinc finger protein 57 Krüppel-like C2H2 zinc finger factors Octamer binding protein ... The secondary structure of CCDC180 is predicted to be almost completely composed of alpha helices, with only a few predicted ... binding factors Nerve growth factor-induced protein C Myc-associated zinc finger GC-binding factor 2 X-box binding protein 1 ... Coiled-coil domain containing protein 180 (CCDC180) is a protein that in humans is encoded by the CCDC180 gene. This protein is ...

https://en.wikipedia.org/wiki/CCDC180

... interaction of CCAAT/enhancer-binding protein with elements flanking the ADH2 TATA box". Gene. 90 (2): 271-9. doi:10.1016/0378- ... Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. GRCh38: ... 2. The primary structure of the gamma 1 protein chain". European Journal of Biochemistry. 145 (3): 447-53. doi:10.1111/j.1432- ... Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibit high ...

https://en.wikipedia.org/wiki/ADH1C

Boruk M, Savory JG, Haché RJ (November 1998). "AF-2-dependent potentiation of CCAAT enhancer binding protein beta-mediated ... The role of alpha and beta isoforms of the glucocorticoid receptor]". Archivos de Bronconeumologia. 38 (9): 436-440. doi: ... the heat shock protein 70 (hsp70) and the protein FKBP4 (FK506-binding protein 4). The endogenous glucocorticoid hormone ... November 1997). "The glucocorticoid receptor is associated with the RNA-binding nuclear matrix protein hnRNP U". The Journal of ...

https://en.wikipedia.org/wiki/Glucocorticoid_receptor

"Regulation of the homeodomain CCAAT displacement/cut protein function by histone acetyltransferases p300/CREB-binding protein ( ... matrix attachment region upstream of the T cell receptor beta gene enhancer binds Cux/CDP and SATB1 and modulates enhancer- ... Interleukin1-alpha, and Cyclo-oxygenase 2 (COX2) genes. Genetic data from over 7,600 cancer patients shows that over 1% has the ... "Regulation of the homeodomain CCAAT displacement/cut protein function by histone acetyltransferases p300/CREB-binding protein ( ...

https://en.wikipedia.org/wiki/CUTL1

Another aspect of the CCAAT binding motif is the CCAAT/enhancer binding proteins (C/EBPs). They are a group of transcription ... The first domain (A1) contains 20 amino acids that forms an alpha helix that appears significant in its interactions with NF-YB ... Protein specific binding is required for the CCAAT box activation. These proteins are known as CCAAT box binding proteins/CCAAT ... Ramji, Dpiak P.; Foka, Pelagia (10 May 2002). "Review Article: CCAAT/enhancer-binding proteins: structure, function and ...

https://en.wikipedia.org/wiki/CAAT_box

TFG-TEC binds to the proximal promoter region of the ENO3 gene. Click on genes, proteins and metabolites below to link to ... activator protein 1 and 2, CCAAT box transcription factor/nuclear factor I, and cyclic AMP. Unlike the other enolase genes, ... and two myocyte-specific enhancer-binding factor 1 boxes. Upstream of the first exon lies a TATA-like box and CpG-rich region, ... A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have ...

https://en.wikipedia.org/wiki/ENO3

Ccaat/Enhancer Binding Protein Vertebrate TATA binding protein factor CCAAT binding factors Activator-, mediator- and TBP- ... with occasional short alpha helix segments. PELE uses eight different prediction programs to compare and confirm predictions, ... factor 1 RXR heterodimer binding sites GATA binding factors Nuclear receptor subfamily 2 factors Octamer binding protein EGR/ ... proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be ...

https://en.wikipedia.org/wiki/Multiple_Epidermal_Growth_Factor-like_Domains_8

It is a member of the CCAAT/enhancer-binding protein (C/EBP) family of DNA-binding transcription factors. The protein functions ... Li G, Mongillo M, Chin KT, Harding H, Ron D, Marks AR, Tabas I (September 2009). "Role of ERO1-alpha-mediated stimulation of ... Qiao D, Im E, Qi W, Martinez JD (June 2002). "Activator protein-1 and CCAAT/enhancer-binding protein mediated GADD153 ... Ramji DP, Foka P (August 2002). "CCAAT/enhancer-binding proteins: structure, function and regulation". The Biochemical Journal ...

https://en.wikipedia.org/wiki/DNA_damage-inducible_transcript_3

For example, CCAAT/enhancer-binding protein β (C/EBPβ), an early adipogenic transcription factor, recruits and requires KMT2D ... Mo R, Rao SM, Zhu YJ (June 2006). "Identification of the MLL2 complex as a coactivator for estrogen receptor alpha". The ... KMT2C and KMT2D are required for the binding of H3K27 acetyltransferases CREB-binding protein (CBP) and/or p300 on enhancers, ... The protein selectively binds enhancer regions based on type of cell and stage of differentiation. During differentiation, ...

https://en.wikipedia.org/wiki/KMT2D

... beta-Barrel alpha-helix transcription factors 4.6 Class: TATA binding proteins 4.6.1 Family: TBP 4.7 Class: HMG-box 4.7.1 ... Transcription factors bind to either enhancer or promoter regions of DNA adjacent to the genes that they regulate. Depending on ... Heteromeric CCAAT factors 4.8.1 Family: Heteromeric CCAAT factors 4.9 Class: Grainyhead 4.9.1 Family: Grainyhead 4.10 Class: ... RAV Cdx protein family DNA-binding protein Inhibitor of DNA-binding protein Mapper(2) Nuclear receptor, a class of ligand ...

https://en.wikipedia.org/wiki/Transcription_factor

Hanlon M, Sealy L (May 1999). "Ras regulates the association of serum response factor and CCAAT/enhancer-binding protein beta ... and Sp1 interact as components of a multiprotein transcriptional complex required for activity of the human cardiac alpha-actin ... This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the ... Serum response factor has been shown to interact with: ASCC3, ATF6, CEBPB, CREB-binding protein, ELK4, GATA4, GTF2F1, GTF2I, ...

https://en.wikipedia.org/wiki/Serum_response_factor

Holland MP, Bliss SP, Berghorn KA, Roberson MS (2004). "A role for CCAAT/enhancer-binding protein beta in the basal regulation ... 2001). "A role for the homeobox protein Distal-less 3 in the activation of the glycoprotein hormone alpha subunit gene in ... Homeobox protein DLX-3 is a protein that in humans is encoded by the DLX3 gene. Dlx3 is a crucial regulator of hair follicle ... Park GT, Denning MF, Morasso MI (2001). "Phosphorylation of murine homeodomain protein Dlx3 by protein kinase C". FEBS Lett. ...

https://en.wikipedia.org/wiki/DLX3

"Modulation of DNA binding properties of CCAAT/enhancer binding protein epsilon by heterodimer formation and interactions with ... Wang D, Westerheide SD, Hanson JL, Baldwin AS (Oct 2000). "Tumor necrosis factor alpha-induced phosphorylation of RelA/p65 on ... Parry GC, Mackman N (Dec 1997). "Role of cyclic AMP response element-binding protein in cyclic AMP inhibition of NF-kappaB- ... Gerritsen ME, Williams AJ, Neish AS, Moore S, Shi Y, Collins T (Apr 1997). "CREB-binding protein/p300 are transcriptional ...

https://en.wikipedia.org/wiki/RELA

DNA-binding domain (DBD) DNA-binding protein (DBP) Any polypeptide or protein containing one or more domains capable of ... enhancer A region of DNA near a gene that can be bound by an activator to increase gene expression or by a repressor to ... alpha helix (α-helix) A common structural motif in the secondary structures of proteins consisting of a right-handed helix ... CCAAT box A highly conserved regulatory DNA sequence located approximately 75 base pairs upstream (i.e. -75) of the site of the ...

https://en.wikipedia.org/wiki/Glossary_of_genetics_(0–L)

CCAAT binding protein, and cAMP-responsive element binding protein. Expression of TMEM128 is also regulated at the gene level ... and GH04J004264 enhancer located upstream of their target gene. TMEM128 sequence also contains many binding sites for various ... The evolution rate is at a medium pace, slower than the fibrinogen alpha chain but faster than cytochrome c, suggesting neither ... "PHYRE2 Protein Fold Recognition Server". www.sbg.bio.ic.ac.uk. Retrieved May 2, 2020. "Transcription factor binding sites for ...

https://en.wikipedia.org/wiki/TMEM128

"CEBPB CCAAT enhancer binding protein beta [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. National Institutes of ... The secondary structure of WDCP Protein Isoform 1 consists of 47 random coils (429 residues, 59.5%), 19 alpha-helices (160 ... SUMOylation can affect protein-protein interactions and affect protein ubiquitination. Palmitoylation is the addition of a ... WDCP Isoform 1 has no transmembrane domains, actin-binding motifs, ER retention motifs, or Golgi transport signals. The protein ...

https://en.wikipedia.org/wiki/WD_Repeat_and_Coiled_Coil_Containing_Protein

The CEBPA gene provides instructions for making a protein called CCAAT enhancer-binding protein alpha. Learn about this gene ... The CEBPA gene provides instructions for making a protein called CCAAT enhancer-binding protein alpha. This protein is a ... The other type of mutation blocks the DNA-binding ability of CCAAT enhancer-binding protein alpha. Impaired DNA binding ... The mutations result in a shorter version of CCAAT enhancer-binding protein alpha. This shortened protein is produced from one ...

https://medlineplus.gov/genetics/gene/cebpa/

Adipocyte differentiation-related protein (ADRP). * CCAAT/enhancer-binding proteins (C/EBP) alpha, beta, and delta ... Proopiomelanocortin (POMC) is converted into alpha-melanocyte-stimulating hormone (alpha-MSH), which acts centrally on the ... Diets with high or low protein content and glycemic index for weight-loss maintenance. N Engl J Med. 2010 Nov 25. 363(22):2102- ... The phenomenon reportedly was evidenced by the peak circulating C-reactive protein levels in individuals with obesity. [43] ...

https://www.medscape.com/answers/123702-11470/how-is-pickwickian-syndrome-related-to-obesity

... transcription factors PU.1 and CEBPA and the chromatin modifier CTCF as well as of the histone markers of promoter and enhancer ... transcription factors PU.1 and CEBPA and the chromatin modifier CTCF as well as of the histone markers of promoter and enhancer ... CCAAT/enhancer binding protein alpha; ChIP-seq, chromatin immunoprecipitation sequencing; CTCF, CCCTC-binding factor; CYP26B1, ... Two CTCF proteins bound at adjacent chromatin boundaries form a complex defining a TAD (right bottom). Enhancers and TSS ...

https://www.frontiersin.org/articles/10.3389/fphys.2019.00194/full

C/EBP alpha. CCAAT/enhancer-binding protein, alpha. fat metabolism. M. musculus. Chiu et al., 2004. ... CCAAT/enhancer-binding protein, beta. fat metabolism. M. musculus. Chiu et al., 2004. ... possible heat shock protein. C. elegans. Yokoyama et al., 2002. JNK. Jun N-terminal protein kinase. stress-activated protein ... telomere-binding protein. C. elegans. Rogina et al., 2002. Ku70. Thyroid-Lupus autoantigen. homologous recombination and ...

https://genomics.senescence.info/genes/function.html

Dive into the research topics of Glycogen synthase kinase-3β-mediated CCAAT/enhancer-binding protein delta phosphorylation in ... Glycogen synthase kinase-3β-mediated CCAAT/enhancer-binding protein delta phosphorylation in astrocytes promotes migration and ...

https://tmu.pure.elsevier.com/en/publications/glycogen-synthase-kinase-3%CE%B2-mediated-ccaatenhancer-binding-protei/fingerprints/

Growth hormone induces CCAAT/enhancer binding protein alpha (C/EBPalpha) in cultured rat hepatocytes. ... A missense mutation in the non-neural G-protein alpha-subunit isoforms modulates susceptibility to obesity. ... Statins inhibit protein lipidation and induce the unfolded protein response in the non-sterol producing nematode Caenorhabditis ... Interleukin-6 Receptor alpha is Co-localised with Melanin-Concentrating Hormone in Human and Mouse Hypothalamus ...

https://www.gu.se/om-universitetet/hitta-person/john-olovjansson

CCAAT/enhancer binding protein (C/EBP), alpha. 05202 Transcriptional misregulation in cancer. K09055 CEBPA; CCAAT/enhancer ... K09055 CEBPA; CCAAT/enhancer binding protein (C/EBP), alpha. 09180 Brite Hierarchies. 09182 Protein families: genetic ... alpha. 09162 Cancer: specific types. 05221 Acute myeloid leukemia. K09055 CEBPA; CCAAT/enhancer binding protein (C/EBP), alpha ... K09055 CEBPA; CCAAT/enhancer binding protein (C/EBP), alpha. Transcription factors [BR:ko03000]. Eukaryotic type. Basic leucine ...

https://www.kegg.jp/entry/K09055

The CCAAT-enhancer-binding protein (C/EBP) transcription factor alpha (C/EBPα) plays an important role in the differentiation ... The alpha chain is responsible for the IgE binding. The beta and the gamma chains contain ITAM motifs that increase FcεRI ... A helix-loop-helix protein that activates transcription through the immunoglobulin enhancer μE3 motif. Genes Dev. 1990, 4, 167- ... GATA2 directly binds to the C/EBPα gene inhibiting its expression; concomitantly the loss of GATA2 activity by DNA binding ...

https://www.mdpi.com/1422-0067/22/9/4935/htm

Different regulation of the LXR-alpha promoter activity by isoforms of CCAAT/enhancer-binding proteins. Biochemical and ... cellular retinol-binding protein, and cellular retinoic acid-binding protein in rat testis: evidence for germ cell-specific ... Cellular retinol binding protein 1 in rat Sertoli cells - Evidence for independent control at the mRNA and protein levels. ... Regulation of mRNA levels for cellular retinol binding protein in rat Sertoli cells by cyclic AMP and retinol. Biochemical and ...

https://www.mn.uio.no/ibv/personer/emeriti/winnie/index.html

B cell identity by either ectopic expression of the myeloid transcription factor CCAAT/enhancer-binding-protein-alpha (C/ ...

https://pubmed.ncbi.nlm.nih.gov/18423197/?dopt=Abstract

CCAAT-Enhancer-Binding Protein-alpha Medicine & Life Sciences 25% * Co-Repressor Proteins Medicine & Life Sciences 22% ... keywords = "CCAAT/ENHANCER-BINDING PROTEINS, RECEPTOR GAMMA-2 PROMOTER, ADIPOCYTE DIFFERENTIATION, 3T3-L1 PREADIPOCYTES, ... INSULIN SENSITIVITY, FUSION PROTEIN, GENE PROMOTER, GROWTH-FACTOR, MICE LACKING, ALPHA-GENE", ...

https://abdn.pure.elsevier.com/en/publications/etomtg8-is-an-inhibitor-of-cebp-beta-activity-and-a-regulator-of-

CCAAT/enhancer-binding protein alpha-Brm complexes. Journal of Biological Chemistry, 282(2):1268-78, Jan 12 2007. ... Age-specific CUGBP1-eIF2 complex increases translation of CCAAT/enhancer-binding protein beta in old liver. Journal of ... through specific activation of RNA CUG binding protein, CUGBP1. Experimental Cell Research, 314(11-12): 2266-78, July 1 2008. ... Wang G-L., Shi X., Salisbury E., and Timchenko N.A. Regulation of apoptotic and growth inhibitory activities of C/EBP alpha in ...

https://www.utmb.edu/ortho/faculty/elizabeth-salisbury-phd

... are located within putative binding sites for the CCAAT/Enhancer Binding Protein (CEBP) and Hepatocyte Nuclear Factor 4, Alpha ... Overlap of 2 suggestive SNPs with likely TF binding sites suggests possible roles in disruption of gene regulation. These ...

http://www2a.cdc.gov/nioshtic-2/BuildQyr.asp?s1=asthma+OR+%27allerg%2A%27+OR+%27immune%2A%27&f1=%2A&Startyear=&Adv=0&terms=1&EndYear=&Limit=10000&sort=&D1=10&PageNo=107&RecNo=1069&View=f&

... induced differentiation depends on a promoter region that contains two CCAAT/Enhancer-binding Protein-β (C/EBPβ) sites. We ... alpha isoform and Pcyt1a gene for CTP:phosphocholine cytidylyltransferase (CCT) alpha isoform. Here we show that CKα is ... We further detect increased IL-6 that amplified JUN-mediated CD47 enhancer activity and protein expression. Using an in vivo ... alpha isoform and the Pcyt1a gene for the CTP:phosphocholine cytidylyltransferase (CCT) alpha isoform. We report that the ...

https://profiles.stanford.edu/pablo-domizi?tab=bio

... while myeloid transcription factors such as CCAAT/enhancer binding protein-alpha (C/EBPalpha), C/EBPdelta, and C/EBPepsilon ... We also explored structure-function relationships using mutant HOXB6 proteins unable to bind to DNA or a key HOX-binding ... Prion protein is expressed on long-term repopulating hematopoietic stem cells and is important for their self-renewal. ... These biologic effects of HOXB6 were largely dependent on DNA binding but independent of direct interaction with PBX1. View ...

https://www.stemcell.com/catalogsearch/result/index/?category_option=Documents&p=2&parent_skus=28600&q=28600&resource_type=Reference

CCAAT enhancer binding protein alpha. 14. If you are already a registered HGMD user, please log in using the button above to ... CCAAT enhancer binding protein alpha. 23. If you are already an HGMD Professional subscriber, please log in using the button ...

https://www.hgmd.cf.ac.uk/ac/gene.php?gene=CEBPA

CDKN2A interacting protein [Source:HGN.... CEBPA. 1050. CEBPA. CCAAT enhancer binding protein alpha [.... ... Cytokine-activated STAT proteins dimerize and bind to high-affinity motifs, and N-terminal domain-mediated oligomerization of ... FERNANDEZ_BOUND_BY_MYC GSE10239_KLRG1INT_VS_KLRG1HIGH_EFF_CD8_TCELL_DN GSE10239_KLRG1INT_VS_KLRG1HIGH_EFF_CD8_TCELL_UP GSE10239 ... dimers allows tetramer formation and binding to low-affinity tandem motifs, but the functions of dimers versus tetramers are ...

https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/GSE36888_UNTREATED_VS_IL2_TREATED_TCELL_17H_UP.html

CCAAT enhancer-binding protein-alpha (C/EBP-alpha), regulatory element-binding protein-1c (SREBP-1c), adipocyte fatty acid- ... CCAAT/enhancer binding protein alpha(C/EBPalpha), the main transcription factor essential for the expression of 11beta-HSD1, ... protein diet ate 12% more calories over four days than they did on a 15% protein diet ... When protein in the diet goes too low ... and adequate protein, low dairy (APLD) differing in the quantity of total dietary protein and dairy food-source protein ...

https://qualitycounts.com/newsletter11/111026.htm

CCAAT-Enhancer-Binding Protein-alpha Entry term(s). C EBP alpha C EBPalpha C-EBP alpha C-EBPalpha CCAAT Enhancer Binding ... CCAAT-Enhancer-Binding Protein-alpha - Preferred Concept UI. M0164348. Scope note. A CCAAT-enhancer-binding protein found in ... C EBP alpha. C EBPalpha. C-EBP alpha. C-EBPalpha. CCAAT Enhancer Binding Protein alpha. ... Experiments with knock-out mice have demonstrated that CCAAT-enhancer binding protein-alpha is essential for the functioning ...

https://decs.bvsalud.org/en/ths/resource/?id=35342

CCAAT/enhancer binding protein alpha (C/EBPα), and sterol regulatory element binding protein-1c]. In addition, SPW-HHPE ... CCAAT/enhancer binding protein alpha (C/EBPα), and sterol regulatory element binding protein-1c]. In addition, SPW-HHPE ... CCAAT/enhancer binding protein alpha (C/EBPα), and sterol regulatory element binding protein-1c]. In addition, SPW-HHPE ... CCAAT/enhancer binding protein alpha (C/EBPα), and sterol regulatory element binding protein-1c]. In addition, SPW-HHPE ...

https://yonsei.pure.elsevier.com/en/publications/high-hydrostatic-pressure-extract-of-siegesbeckia-orientalis-inhi

https://www.staging.medscape.com/answers/123702-11491/what-is-the-role-of-genetic-susceptibility-loci-in-the-development-of-obesity

... a wiki resource centered on human protein-protein interactions ... CCAAT enhancer binding protein alpha. Image. No pdb structure. ... DNA-binding Transcription Factor Activity, RNA Polymerase II-specific. *DNA-binding Transcription Activator Activity, RNA ... RNA Polymerase II Cis-regulatory Region Sequence-specific DNA Binding. *DNA-binding Transcription Factor Activity, RNA ... Transcription Regulatory Region Sequence-specific DNA Binding. *RNA Polymerase II Cis-regulatory Region Sequence-specific DNA ...

https://hagrid.dbmi.pitt.edu/wiki-MPM/index.php/pair/view/6663/1050

Acute myeloid leukemia with CCAAT/enhancer binding protein alpha somatic mutation Active Synonym false false ... Acute myeloid leukaemia with CCAAT/enhancer binding protein alpha somatic mutation Active Synonym false false ... Acute myeloid leukemia with CCAAT/enhancer binding protein alpha somatic mutation (disorder). ... Acute myeloid leukemia with CCAAT/enhancer binding protein alpha somatic mutation (disorder). ...

https://phinvads.cdc.gov/vads/ViewCodeSystemConcept.action?oid=2.16.840.1.113883.6.96&code=764855007

ccaat/enhancer binding protein alpha (3) Date. Filter by date. Date range ... AXL Expression in Pediatric AML Is Associated with Putative LSC and Correlates with a Distinct Set of Proteins Associated with ... Proteomics in Pediatric Acute Myeloid and T-Cell Lymphoblastic Leukemia: Shared Individual Protein Expression Patterns Co- ... Cell Metabolism, Cell Cycle, and Unfolded Protein Response Fieke W Hoff, BSc, Peter P. Ruvolo, PhD, Yihua Qiu, Michael Andreeff ...

https://ashpublications.org/blood/search-results?f_AllAuthors=Fieke+W+Hoff%2C+BSc

CEBPA (CCAAT enhancer binding protein alpha). Chromosome. 19. Transcript - NCBI ID. NM_004364.4. ... Protein: Description of variant at protein level (following HGVS recommendations).. *p.(Arg345Pro) = change predicted from DNA ... Affects function: The variants effect on the proteins function, in the format Reported/Curator concluded; + indicating the ...

http://www.inc.gob.ar/sither/transcripts/00000073

CCAAT-Enhancer-Binding Protein-alpha 69% * Messenger RNA 68% * Adipogenesis 58% * HIV-Associated Lipodystrophy Syndrome 42% ... HIV-1 viral protein R (Vpr) induces fatty liver in mice via LXRα and PPARα dysregulation: Implications for HIV-specific ... Can serum surfactant protein d or cc-chemokine ligand 18 predict outcome of interstitial lung disease in patients with early ... CD25 and CD69 induction by α4β1 outside-in signalling requires TCR early signalling complex proteins. Cimo, A. M., Ahmed, Z., ...

https://scholars.houstonmethodist.org/en/persons/dorothy-e-lewis/publications/?ordering=publicationYearThenTitle&descending=true

CCAAT/enhancer binding protein (C/EBP), alpha. 0.042. Rad21. RAD21 homolog (S. pombe). 0.038. ...

http://imp.princeton.edu/predictions/process/mouse-context-global/15127/?gene=75705

CCAAT/enhancer binding protein (C/EBP), alpha. U: 4. D: 1. CPE. carboxypeptidase E. U: 4 ... Results for query: protein domain specific binding. Gene Symbol. Gene Name. Links to Senescence. ... protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I). U: 2 ... ATP-binding cassette, sub-family C (CFTR/MRP), member 2. U: 1 ... growth factor receptor bound protein 2-associated protein 2. D ...

https://senequest.net/search?type=go_term&search=protein%20domain%20specific%20binding

CCAAT/Enhancer-Binding Protein alpha (CEBPα), Peroxisome proliferator-activated receptor gamma (PPARγ), UCP1, Leptin (LEP), and ... CCAAT/Enhancer-Binding Protein alpha (CEBPα), Peroxisome proliferator-activated receptor gamma (PPARγ), UCP1, Leptin (LEP), and ... CCAAT/Enhancer-Binding Protein alpha (CEBPα), Peroxisome proliferator-activated receptor gamma (PPARγ), UCP1, Leptin (LEP), and ... CCAAT/Enhancer-Binding Protein alpha (CEBPα), Peroxisome proliferator-activated receptor gamma (PPARγ), UCP1, Leptin (LEP), and ...

https://experts.unthsc.edu/en/publications/comparative-analysis-of-human-adipose-derived-stromalstem-cells-a

The CEBPA gene provides instructions for making a protein called CCAAT enhancer-binding protein alpha. (medlineplus.gov)
This shortened protein is produced from one copy of the CEBPA gene in each cell, and it is believed to interfere with the tumor suppressor function of the normal protein produced from the second copy of the gene. (medlineplus.gov)
The somatic CEBPA gene mutations that have been identified in leukemia cells generally decrease the DNA-binding ability of CCAAT enhancer-binding protein alpha. (medlineplus.gov)
This includes the 1,25(OH) 2 D 3 -modulated cistromes of VDR, the pioneer transcription factors PU.1 and CEBPA and the chromatin modifier CTCF as well as of the histone markers of promoter and enhancer regions, H3K4me3 and H3K27ac, respectively. (frontiersin.org)
Book Enhancer Binding Protein Alpha (CEBPA) Mutation Detection Test at the best price in Delhi NCR, India from GDIC (Ganesh Diagnostic & Imaging Centre). (ganeshdiagnostic.com)
CEBPA (CCAAT/enhancer binding protein) is a gene that encodes a transcription factor involved in granulocyte differentiation. (ganeshdiagnostic.com)
What is the procedure for Enhancer Binding Protein Alpha (CEBPA) Mutation Detection Test? (ganeshdiagnostic.com)
The Enhancer Binding Protein Alpha ( CEBPA) Mutation Detection Test costs vary by region and facility. (ganeshdiagnostic.com)
PRKCH can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). (mybio.ie)

Several derivatives of 5-aminoimidazole-4-carboxamide-1--d-ribofuranoside (AICAR), an AMP-activated protein kinase (AMPK) activator, were identified as transcriptional activators of the DDT gene. (tokushima-u.ac.jp)
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. (mybio.ie)
PRKCH (Protein Kinase C Eta), a calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. (mybio.ie)
In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. (mybio.ie)
AMP-activated proteins kinase (AMPK) signaling may be the crucial pathway purchase VX-950 of lipid rate of metabolism. (casein-kinase.info)

This protein is a transcription factor, which means that it attaches (binds) to specific regions of DNA and helps control the activity (expression) of certain genes. (medlineplus.gov)
However, reprogramming of mature B cells required additional interruption with the transcriptional state maintaining B cell identity by either ectopic expression of the myeloid transcription factor CCAAT/enhancer-binding-protein-alpha (C/EBPalpha) or specific knockdown of the B cell transcription factor Pax5. (nih.gov)
It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. (nih.gov)
Likewise, keratin also upregulated osteogenic markers such as type I collagen, alpha 1 (COL1A1), runt-related transcription factor 2 (RUNX2), and vitamin D receptor (VDR) to hASCs. (ntu.edu.tw)

CCAAT enhancer-binding protein alpha is involved in the maturation (differentiation) of certain blood cells. (medlineplus.gov)
Experiments with knock-out mice have demonstrated that CCAAT-enhancer binding protein-alpha is essential for the functioning and differentiation of HEPATOCYTES and ADIPOCYTES . (bvsalud.org)
Relative to untreated polystyrene plates, keratin-coating increased the mRNA levels of lipoprotein lipase (LPL), peroxisome proliferator-activated receptor gamma (PPAR- £^), and CCAAT-enhancer binding protein alpha (CEBP-£\) to hASCs, which reveals an improvement in adipogenic differentiation. (ntu.edu.tw)
Included in this, CCAAT enhancer binding proteins alpha (C/EBP), sterol regulatory component binding proteins 1c (SREBP-1c), and peroxide proliferative activation receptor gamma (PPAR) are fundamental transcription elements in charge of end-phase differentiation and rules of a lot of downstream genes involved with lipid rate of metabolism [17,18]. (casein-kinase.info)

Impaired DNA binding interferes with the protein's ability to regulate gene expression and impairs its tumor suppressor function. (medlineplus.gov)
NCU-G1 "knockout" musen ble skapt ved innsetting av en "gene-trap" i første intron som fører til stopp i transkripsjonen etter første ekson. (uio.no)
Overlap of 2 suggestive SNPs with likely TF binding sites suggests possible roles in disruption of gene regulation. (cdc.gov)
Retroviral vectors with long terminal repeats (LTRs), which contain strong enhancer/promoter sequences at both ends of their genome, are widely used for stable gene transfer into hematopoietic cells. (stemcell.com)
CCAAT/enhancer binding protein alpha, beta and delta gene variants: associations with obesity related phenotypes in the Leeds Family Study. (cdc.gov)
Tumour necrosis factor-alpha regulates expression of the CCAAT-enhancer-binding proteins (C/EBPs) alpha and beta and determines the occupation of the C/EBP site in the promoter of the insulin-responsive glucose-transporter gene in 3T3-L1 adipocytes. (ecu.edu)
We have demonstrated previously that treatment of 3T3-L1 adipocytes with tumour necrosis factor-alpha (TNF) results in a rapid (4 h) and significant (75-80%) reduction in the rate of transcription of the GLUT4 gene. (ecu.edu)
Description of the protein which includes the UniProt Function and the NCBI Gene Summary. (nih.gov)
Such introductions of foreign nucleic acid using various chemical, biological, or physical methods can result in a change of the properties of the cell, allowing the study of gene function and protein expression in the context of the cell. (cdc.gov)

Self-inactivating (SIN) retroviral vectors do not contain the terminal repetition of the enhancer/promoter, theoretically attenuating the interaction with neighboring cellular genes. (stemcell.com)
With a new assay based on in vitro expansion of primary murine hematopoietic cells and selection in limiting dilution, we showed that SIN vectors using a strong internal retroviral enhancer/promoter may also transform cells by insertional mutagenesis. (stemcell.com)
binding site over the iNOS promoter. (academiaperuanadepsicologia.org)
As opposed to the indigenous toxin, a catalytically inactivated SubAB mutant slightly enhanced LPS-induced iNOS binding and appearance of NF-B subunits towards the iNOS promoter. (academiaperuanadepsicologia.org)

Various cell types can process and present these antigens to T cells, or antigens may be soluble and bound to B-cell receptors. (medscape.com)
SubAB binds to receptors in the cell membrane (59, 60) and thus gets into the cell, producing a site-specific cleavage of endoplasmic reticulum (ER) chaperone proteins BiP/Grp78. (academiaperuanadepsicologia.org)
SREBP-1c raises intracellular cholesterol concentrations by improving the purchase VX-950 manifestation of cholesterol-binding receptors for purchase VX-950 the cell membrane surface area [20]. (casein-kinase.info)

A 729-bp open reading frame (gltR) was identified in Pseudomonas aeruginosa PAO1 that encodes a product homologous to the two-component response regulator family of proteins. (ecu.edu)
The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. (nih.gov)
The effects of ER stress were transcriptional because of downregulation of CAAT/enhancer binding protein-α and peroxisome proliferator-activated receptor-γ transcriptional activators and upregulation of the transcriptional repressor CAAT/enhancer binding protein homologous protein-10 (CHOP10). (diabetesjournals.org)

Functional genomics data indicate that 2 of the suggestive SNPs (rs2446823 and rs2446824) are located within putative binding sites for the CCAAT/Enhancer Binding Protein (CEBP) and Hepatocyte Nuclear Factor 4, Alpha transcription factors (TFs), respectively. (cdc.gov)
Binding of complement to a foreign substance, or antigen, amplifies and augments the body's innate immune system by means of its role as an opsonin (a factor that enhances phagocytosis of unwanted particles) and as a chemoattractant (a factor that recruits cells to areas of inflammation). (medscape.com)

From inside the an aggressive binding apparatus, the fresh new methyl-CpG binding proteins (MeCP2) 1st binds methylated CpG websites and then prevents web sites to other protein so you can join [20, 21]. (casadediosusa.net)

The SubAB influence on LPS-induced iNOS appearance was significantly low in macrophages from NF-B1 (p50)-lacking mice, which lacked a DNA-binding subunit from the p65/p50 heterodimer, recommending that p50 was involved Cucurbitacin I with SubAB-mediated inhibition of iNOS appearance. (academiaperuanadepsicologia.org)

Absence of the tumor suppressor function of CCAAT enhancer-binding protein alpha is believed to disrupt the regulation of blood cell production, leading to the uncontrolled production of abnormal cells that occurs in acute myeloid leukemia. (medlineplus.gov)

QRT-PCR method was used to test the expression level of microRNA-199a and key adipogenic transcription factors, including peroxisome proliferator activated receptor gamma2 (PPARγ2), CCAAT/enhancer binding proteins alpha (C/EBPα), adipocyte fatty acid-binding protein (aP2). (scholars.direct)

Opsonins are often antibodies or components of the complement pathway that bind to the surface of target organisms to facilitate this phagocytosis. (medscape.com)

Cytokine-activated STAT proteins dimerize and bind to high-affinity motifs, and N-terminal domain-mediated oligomerization of dimers allows tetramer formation and binding to low-affinity tandem motifs, but the functions of dimers versus tetramers are unknown. (gsea-msigdb.org)

SPW-HHPE reduced the mRNA and protein expression of adipogenic regulatory factors [peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer binding protein alpha (C/EBPα), and sterol regulatory element binding protein-1c]. (elsevier.com)
Some protein located downstream from the AMPK pathway, including acetyl coenzyme A carboxylase 1 (ACC1), sterol regulatory component binding proteins 1c (SREBP-1c), blood sugar transporter 4 (GLUT-4), and carnitine palmitoyltransferase 1A (CPT1), are involved with lipid rate of metabolism highly. (casein-kinase.info)

Identification of protease serine S1 family member 53 as a mitochondrial protein in murine islet beta cells, Islets, Vol.14, No.1, 1-13, 2021. (tokushima-u.ac.jp)
SubAB inhibition of TNF-induced NF-B activation in rat renal tubular epithelial cells resulted from induction of CCAAT/enhancer-binding proteins beta (C/EBP) and a mammalian focus on of rapamycin (mTOR)-reliant Akt phosphorylation pathways (37). (academiaperuanadepsicologia.org)

Chemotaxis of neutrophils involves movement of pseudopodia and polymerization of cytoskeletal proteins or actin. (medscape.com)

XV" YOL105C 1 15 18 YOL105C "Putative integral membrane protein containing novel cysteine motif. (davidson.edu)

1] Neutrophils move to the site of invasion by means of chemotaxis, which occurs in response to microbial products, activated complement proteins, and cytokines. (medscape.com)

Triptolide inhibits IL-12/IL-23 expression in APCs via CCAAT/enhancer-binding protein alpha. (cornell.edu)

Lipid storage space in white adipose cells (WAT) is managed by the powerful procedures of lipogenesis and lipolysis, which involve many transcription elements, enzymes, and protein. (casein-kinase.info)

Furthermore, skeletal muscle tissue proteins degradation procedures via lysosomal pathways and ubiquitinCproteasome systems play an important role in muscle tissue atrophy and so are overactive in over 50% of tumor sufferers [8]. (baxkyardgardener.com)

Our results demonstrate that ARMC5 was a substrate adaptor protein between full-length SREBF and CUL3-based E3 ligase, and they suggest the involvement of the SREBF pathway in the development of BMAH. (bvsalud.org)

One type leads to production of an abnormally short protein that interferes with the tumor suppressor function of normal versions of CCAAT enhancer-binding protein alpha. (medlineplus.gov)
Intriguingly, furthermore to these actions, some recent studies demonstrated that SubAB pretreatment of varied cell lines inhibited lipopolysaccharide (LPS)- and tumor necrosis aspect alpha (TNF-)-induced NF-B activation (17, 37). (academiaperuanadepsicologia.org)

Additionally, the result is certainly exacerbated by significant metabolic adjustments, such as for example energy expenses at rest and disturbed fat burning capacity of carbohydrates, protein, and lipids [3]. (baxkyardgardener.com)

After a complex process of education and maturation, a circulating lymphocyte can bind to an antigen. (medscape.com)

Salisbury E.A, Lazard Z.W., Ubogu E.E., Davis A.R., Olmsted-Davis E.A. Transient brown adipocyte-like cells derive from peripheral nerve progenitors in response to bone morphogenetic protein 2. (utmb.edu)
Currently, I am focus on integrating single cell RNA and protein expression data to develop models to predict patient at risk for Antigen Loss relapse after CAR T cells immunotherapy. (stanford.edu)
Prion protein is expressed on long-term repopulating hematopoietic stem cells and is important for their self-renewal. (stemcell.com)
Although the wild-type prion protein (PrP) is abundant and widely expressed in various types of tissues and cells, its physiological function(s) remain unknown, and PrP knockout mice do not exhibit overt and undisputed phenotypes. (stemcell.com)
Transfection is performed to express a protein of interest in cultured cells. (cdc.gov)

CXXC motif containing zinc binding pro. (gsea-msigdb.org)

The other type of mutation blocks the DNA-binding ability of CCAAT enhancer-binding protein alpha. (medlineplus.gov)

Description: A sandwich quantitative ELISA assay kit for detection of Mouse Tubulin Polymerization Promoting Protein (TPPP) in samples from tissue homogenates or other biological fluids. (1elisakits.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tubulin Polymerization Promoting Protein (TPPP) in tissue homogenates, cell lysates and other biological fluids. (1elisakits.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Tubulin Polymerization Promoting Protein (TPPP) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (1elisakits.com)

PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. (mybio.ie)

Evaluations of lineage-specific genetic markers and proteins revealed that the adipogenic, osteogenic and chondrogenic differentiations of hASCs can be successfully induced, which suggested that the stemness of hASCs was maintained when cultured on keratin-coating substrates. (ntu.edu.tw)

Recombinant PRKCH protein was expressed in baculovirus expression system as the full length protein (accession number NP_006246.2) with a N-terminal FLAG tag. (mybio.ie)
The mRNA expression of bone morphogenetic protein 6 (BMP6) and CCAAT/enhancer binding protein alpha (C/EBPα), which are regulators of hepcidin, was also down-regulated in the livers of ANG II-treated mice. (tokushima-u.ac.jp)
The effects of endoplasmic stress inducers on resistin mRNA and secreted protein levels were examined in differentiated 3T3-L1 adipocytes, focusing on the expression and genomic binding of transcriptional regulators of resistin. (diabetesjournals.org)

contains a zinc finger,XV" YOL091W 1 15 16 YOL091W "involved in sporulation,XV" YOL103W-B 1 15 17 YOL103W-B "TyB Gag-Pol protein. (davidson.edu)

Oncological sufferers have reduced bodyweight because of the gradual reduction in muscle tissue and fats mass, while non-muscle protein are conserved [3]. (baxkyardgardener.com)
The function of brown adipose tissue is to directly transfer energy from nutrients to heat by uncoupling protein (UCP) 1, which mediates uncoupling of oxidative phosphorylation from ATP synthesis (conferred thermogenesis) [ 8 - 10 ]. (hindawi.com)

Previous research show that BiP/Grp78 cleavage by SubAB initiates an ER stress-induced unfolded proteins response (UPR) (41, 54), leading to transient inhibition of proteins synthesis (34), G0/G1 cell routine arrest (33, 34), downregulation of distance junction appearance (24), and caspase-dependent apoptosis via mitochondrial membrane harm (32, 58). (academiaperuanadepsicologia.org)

ER stress is a potent regulator of resistin, suggesting that ER stress may underlie the local downregulation of resistin mRNA and protein in fat in murine obesity. (diabetesjournals.org)

The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. (nih.gov)

calcium/calmodulin dependent protein k. (gsea-msigdb.org)

concentration of specific protein per volume of liquid. (cdc.gov)

CEBPA gene: MedlinePlus Genetics (medlineplus.gov)

Frontiers | Primary Vitamin D Target Genes of Human Monocytes (frontiersin.org)

Familial acute myeloid leukemia with mutated CEBPA: MedlinePlus Genetics (medlineplus.gov)

Functional Clustering of Genes Influencing Ageing (genomics.senescence.info)

Regulation of hemopoiesis - Ontology Report - Rat Genome Database (rgd.mcw.edu)

Acute Myeloid Leukemia (AML): Practice Essentials, Pathophysiology, Etiology (medscape.com)

IJMS | Free Full-Text | Anaphylaxis: Focus on Transcription Factor Activity (mdpi.com)

Frontiers | A CREB1 Gene Polymorphism (rs2253206) Is Associated with Prospective Memory in a Healthy Cohort (frontiersin.org)

ETO/MTG8 is an inhibitor of C/EBP beta activity and a regulator of early adipogenesis<... (abdn.pure.elsevier.com)

Pablo Domizi, Ph.D.'s Profile | Stanford Profiles (profiles.stanford.edu)

Anatomy20

Organisms4

Diseases4

Chemicals and Drugs41

Analytical, Diagnostic and Therapeutic Techniques and Equipment11

Phenomena and Processes32

Disciplines and Occupations1

Information Science3

Thapsigargin suppresses phorbol ester-dependent human involucrin promoter activity by suppressing CCAAT-enhancer-binding protein alpha (C/EBPalpha) DNA binding. (1/560)

Identification and characterization of leptin-containing intracellular compartment in rat adipose cells. (2/560)

Role of C/EBP homologous protein (CHOP-10) in the programmed activation of CCAAT/enhancer-binding protein-beta during adipogenesis. (3/560)

Sequential repression and activation of the CCAAT enhancer-binding protein-alpha (C/EBPalpha ) gene during adipogenesis. (4/560)

CREB (cAMP response element binding protein) and C/EBPalpha (CCAAT/enhancer binding protein) are required for the superstimulation of phosphoenolpyruvate carboxykinase gene transcription by adenoviral E1a and cAMP. (5/560)

The rat ortholog of the presumptive flounder antifreeze enhancer-binding protein is a helicase domain-containing protein. (6/560)

Interferon-gamma-induced regulation of peroxisome proliferator-activated receptor gamma and STATs in adipocytes. (7/560)

Hormonal signaling and transcriptional control of adipocyte differentiation. (8/560)

CCAAT-Enhancer-Binding Protein-alpha

CCAAT-Enhancer-Binding Proteins

CCAAT-Enhancer-Binding Protein-beta

CCAAT-Enhancer-Binding Protein-delta

DNA-Binding Proteins

Adipocytes

Promoter Regions, Genetic

Nuclear Proteins

Transcription Factors

Adipogenesis

Cell Differentiation

Enhancer Elements, Genetic

3T3-L1 Cells

Gene Expression Regulation

Granulocytes

Transcriptional Activation

3T3 Cells

RNA, Messenger

PPAR gamma

Gene Expression Regulation, Leukemic

Base Sequence

Leukemia, Myeloid, Acute

Transcription, Genetic

Cell Line

Liver

Transcription Factor CHOP

Mutation

Molecular Sequence Data

Transfection

Electrophoretic Mobility Shift Assay

Binding Sites

Down-Regulation

Protein Binding

Adipose Tissue

Cells, Cultured

Response Elements

Transcription Factor AP-2

Gene Expression

Cell Nucleus

Myelopoiesis

Signal Transduction

GTP-Binding Protein alpha Subunits

Genes, Reporter

DNA

Blotting, Western

Chromatin Immunoprecipitation

Trans-Activators

Tumor Cells, Cultured

Up-Regulation

DNA Primers

Mice, Knockout

Luciferases

Gene Expression Regulation, Enzymologic

Leucine Zippers

Protein Isoforms

Endrin

Regulatory Sequences, Nucleic Acid

Hepatocyte Nuclear Factor 3-beta

Amino Acid Sequence

HeLa Cells

Reverse Transcriptase Polymerase Chain Reaction

Acute-Phase Reaction

NF-kappa B p50 Subunit

Mice, Transgenic

Mice, Inbred C57BL

U937 Cells

Carrier Proteins

Cell Line, Tumor

Dexamethasone

RNA Polymerase Sigma 54

Cyclic AMP Response Element-Binding Protein

Recombinant Fusion Proteins

Phosphorylation

Receptors, Cytoplasmic and Nuclear

NF-kappa B

Blotting, Northern

Glucocorticoids

Oligonucleotides

Mutagenesis, Site-Directed

RNA, Small Interfering