Chemicals and DrugsPhenomena and ProcessesInformation Science
CCAAT-Binding FactorCCAAT-Enhancer-Binding ProteinsCore Binding FactorsNFI Transcription FactorsY-Box-Binding Protein 1DNA-Binding ProteinsTranscription FactorsBase SequencePromoter Regions, GeneticMolecular Sequence DataCore Binding Factor beta SubunitPol1 Transcription Initiation Complex ProteinsCore Binding Factor alpha SubunitsTelomeric Repeat Binding Protein 1G-Box Binding FactorsCCAAT-Enhancer-Binding Protein-betaTranscription, GeneticCore Binding Factor Alpha 2 Subunit
CCAAT-Binding Factor
A heterotrimeric DNA-binding protein that binds to CCAAT motifs in the promoters of eukaryotic genes. It is composed of three subunits: A, B and C.
CCAAT-Enhancer-Binding Proteins
A class of proteins that were originally identified by their ability to bind the DNA sequence CCAAT. The typical CCAAT-enhancer binding protein forms dimers and consists of an activation domain, a DNA-binding basic region, and a leucine-rich dimerization domain (LEUCINE ZIPPERS). CCAAT-BINDING FACTOR is structurally distinct type of CCAAT-enhancer binding protein consisting of a trimer of three different subunits.
Core Binding Factors
Heterodimeric transcription factors containing a DNA-binding alpha subunits, (CORE BINDING FACTOR ALPHA SUBUNITS), along with a non-DNA-binding beta subunits, CORE BINDING FACTOR BETA SUBUNIT. Core Binding Factor regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.
NFI Transcription Factors
Transcription factors that were originally identified as site-specific DNA-binding proteins essential for DNA REPLICATION by ADENOVIRUSES. They play important roles in MAMMARY GLAND function and development.
Y-Box-Binding Protein 1
Y-box-binding protein 1 was originally identified as a DNA-binding protein that interacts with Y-box PROMOTER REGIONS of MHC CLASS II GENES. It is a highly conserved transcription factor that regulates expression of a wide variety of GENES.
DNA-Binding Proteins
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Transcription Factors
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Base Sequence
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Promoter Regions, Genetic
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Core Binding Factor beta Subunit
A non-DNA binding transcription factor that is a subunit of core binding factor. It forms heterodimeric complexes with CORE BINDING FACTOR ALPHA SUBUNITS, and regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.
Pol1 Transcription Initiation Complex Proteins
Factors that form a preinitiation complex at promoters that are specifically transcribed by RNA POLYMERASE I.
Core Binding Factor alpha Subunits
A family of transcription factors that bind to the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. Family members contain a highly conserved DNA-binding domain known as the runt domain. They can act as both activators and repressors of expression of GENES involved in CELL DIFFERENTIATION and CELL CYCLE progression.
Telomeric Repeat Binding Protein 1
A ubiquitously expressed telomere-binding protein that is present at TELOMERES throughout the CELL CYCLE. It is a suppressor of telomere elongation and may be involved in stabilization of telomere length. It is structurally different from TELOMERIC REPEAT BINDING PROTEIN 2 in that it contains acidic N-terminal amino acid residues.
G-Box Binding Factors
A family of transcription factors found primarily in PLANTS that bind to the G-box DNA sequence CACGTG or to a consensus sequence CANNTG.
CCAAT-Enhancer-Binding Protein-beta
A CCAAT-enhancer-binding protein found in LIVER; INTESTINES; LUNG and ADIPOSE TISSUE. It is an important mediator of INTERLEUKIN-6 signaling.
Transcription, Genetic
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Core Binding Factor Alpha 2 Subunit
A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain. Runx1 is frequently mutated in human LEUKEMIAS.

Transcriptional regulation of the squalene synthase gene (ERG9) in the yeast Saccharomyces cerevisiae. (1/416)

The ergosterol biosynthetic pathway is a specific branch of the mevalonate pathway. Since the cells requirement for sterols is greater than for isoprenoids, sterol biosynthesis must be regulated independently of isoprenoid biosynthesis. In this study we explored the transcriptional regulation of squalene synthase (ERG9) in Saccharomyces cerevisiae, the first enzyme dedicated to the synthesis of sterols. A mutant search was performed to identify genes that were involved in the regulation of the expression of an ERG9-lacZ promoter fusion. Mutants with phenotypes consistent with known sterol biosynthetic mutations (ERG3, ERG7, ERG24) increased expression of ERG9. In addition, treatment of wild-type cells with the sterol inhibitors zaragozic acid and ketoconazole, which target squalene synthase and the C-14 sterol demethylase respectively, also caused an increase in ERG9 expression. The data also demonstrate that heme mutants increased ERG9 expression while anaerobic conditions decreased expression. Additionally, the heme activator protein transcription factors HAP1 and HAP2/3/4, the yeast activator protein transcription factor yAP-1, and the phospholipid transcription factor complex INO2/4 regulate ERG9 expression. ERG9 expression is decreased in hap1, hap2/3/4, and yap-1 mutants while ino2/4 mutants showed an increase in ERG9 expression. This study demonstrates that ERG9 transcription is regulated by several diverse factors, consistent with the idea that as the first step dedicated to the synthesis of sterols, squalene synthase gene expression and ultimately sterol biosynthesis is highly regulated.  (+info)

Down-regulation of cyclin B1 gene transcription in terminally differentiated skeletal muscle cells is associated with loss of functional CCAAT-binding NF-Y complex. (2/416)

The observation that cyclin B1 protein and mRNAs are down-regulated in terminally differentiated (TD) C2C12 cells, suggested us to investigate the transcriptional regulation of the cyclin B1 gene in these cells. Transfections of cyclin B1 promoter constructs indicate that two CCAAT boxes support cyclin B1 promoter activity in proliferating cells. EMSAs demonstrate that both CCAAT boxes are recognized by the trimeric NF-Y complex in proliferating but not in TD cells. Transfecting a dominant-negative mutant of NF-YA we provide evidence that NF-Y is required for maximal promoter activity. Addition of recombinant NF-YA to TD C2C12 nuclear extracts restores binding activity in vitro, thus indicating that the loss of NF-YA in TD cells is responsible for the lack of the NF-Y binding to the CCAAT boxes. Consistent with this, we found that the NF-YA protein is absent in TD C2C12 cells. In conclusion, our data demonstrate that NF-Y is required for cyclin B1 promoter activity. We also demonstrate that cyclin B1 expression is regulated at the transcriptional level in TD C2C12 cells and that the switch-off of cyclin B1 promoter activity in differentiated cells depends upon the loss of a functional NF-Y complex. In particular the loss of NF-YA protein is most likely responsible for its inactivation.  (+info)

Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays. (3/416)

The yeast SWI/SNF complex is required for the transcription of several yeast genes and has been shown to alter nucleosome structure in an ATP-dependent reaction. In this study, we show that the complex stimulated in vitro transcription from nucleosome templates in an activation domain-dependent manner. Transcription stimulation by SWI/SNF required an activation domain with which it directly interacts. The acidic activation domains of VP16, Gcn4, Swi5, and Hap4 interacted directly with the purified SWI/SNF complex and with the SWI/SNF complex in whole-cell extracts. The similarity of activation domain interactions and transcriptional stimulation between SWI/SNF and the SAGA histone acetyltransferase complex may account for their apparent overlapping functions in vivo.  (+info)

Identification of proteins that interact with NF-YA. (4/416)

We used the yeast two-hybrid system to show that the serum response factor (SRF) and zinc-fingers and homeobox 1 (ZHXI) proteins interact with the A subunit of nuclear factor-Y (NF-YA). GST pulldown assays revealed that both proteins interact specifically with NF-YA in vitro. Amino acids located between 272 and 564, a region that contains two homeodomains, are required for the interaction of ZHX1 with NF-YA. Two different domains of NF-YA, a glutamine-rich region and a serine/threonine-rich region, are necessary for the interactions with ZHX1 and SRF, respectively.  (+info)

A nuclear factor Y (NFY) site positively regulates the human CD34 stem cell gene. (5/416)

Proper regulation of the human CD34 gene requires a combinatorial action of multiple proximal and long-range, cis elements. This report shows that, like the murine CD34 5' untranslated region (UTR), the corresponding region of the human CD34 gene is necessary for optimal promoter activity. We localized the most critical element of this region to base pairs +48/+75. Through oligonucleotide competition and antibody supershift experiments in electrophoretic mobility shift assays, we found that this sequence contains a binding site (CCAAT box) for the transcription factor NFY (nuclear factor Y), a factor mediating cell type-specific and cell-cycle regulated expression of genes. Mutating this site led to a 5-fold decrease in CD34 promoter activity in transient transfection experiments. Interestingly, NFY binds adjacently to the earlier identified c-myb binding site. Here we show that both binding sites are important for CD34 promoter function: mutating either site alone decreased CD34 promoter-driven reporter gene activity 4-fold. We also show that the integrity of the c-myb binding site is necessary for stabilization of NFY binding to its site. Such cooperation between c-myb, which is expressed in early hematopoietic cells, and NFY, which is expressed in many cell types, might contribute to specific activation of CD34 in stem cells. The CCAAT box motif was also noted in the 5' UTR of the murine CD34 gene, however, NFY did not bind to this region. Thus, our results indicate that the functional similarities between the human and murine CD34 5' UTRs are achieved through different molecular mechanism(s).  (+info)

Identification and characterization of basal and cyclic AMP response elements in the promoter of the rat GTP cyclohydrolase I gene. (6/416)

5812 base pairs of rat GTP cyclohydrolase I (GTPCH) 5'-flanking region were cloned and sequenced, and the transcription start site was determined for the gene in rat liver. Progressive deletion analysis using transient transfection assays of luciferase reporter constructs defined the core promoter as a highly conserved 142-base pair GC-rich sequence upstream from the cap site. DNase I footprint analysis of this region revealed (5' --> 3') a Sp1/GC box, a noncanonical cAMP-response element (CRE), a CCAAT-box, and an E-box. Transcription from the core promoter in PC12 but not C6 or Rat2 cells was enhanced by incubation with 8-bromo-cyclic AMP. Mutagenesis showed that both the CRE and CCAAT-box independently contribute to basal and cAMP-dependent activity. The combined CRE and CCAAT-box cassette was also found to enhance basal transcription and confer cAMP sensitivity on a heterologous minimal promoter. The addition of the Sp1/GC box sequence to this minimal promoter construct inhibited basal transcription without affecting the cAMP response. EMSA showed that nuclear proteins from PC12 but not C6 or Rat2 cells bind the CRE as a complex containing activating transcription factor (ATF)-4 and CCAAT enhancer-binding protein beta, while both PC12 and C6 cell nuclear extracts were recruited by the CCAAT-box as a complex containing nuclear factor Y. Overexpression of ATF-4 in PC12 cells was found to transactivate the GTPCH promoter response to cAMP. These studies suggest that the elements required for cell type-specific cAMP-dependent enhancement of gene transcription are located along the GTPCH core promoter and include the CRE and adjacent CCAAT-box and the proteins ATF-4, CCAAT enhancer-binding protein beta, and nuclear factor Y.  (+info)

Sterol regulation of human fatty acid synthase promoter I requires nuclear factor-Y- and Sp-1-binding sites. (7/416)

To understand cholesterol-mediated regulation of human fatty acid synthase promoter I, we tested various 5'-deletion constructs of promoter I-luciferase reporter gene constructs in HepG2 cells. The reporter gene constructs that contained only the Sp-1-binding site (nucleotides -82 to -74) and the two tandem sterol regulatory elements (SREs; nucleotides -63 to -46) did not respond to cholesterol. Only the reporter gene constructs containing a nuclear factor-Y (NF-Y) sequence, the CCAAT sequence (nucleotides -90 to -86), an Sp-1 sequence, and the two tandem SREs responded to cholesterol. The NF-Y-binding site, therefore, is essential for cholesterol response. Mutating the SREs or the NF-Y site and inserting 4 bp between the Sp-1- and NF-Y-binding sites both resulted in a minimal cholesterol response of the reporter genes. Electrophoretic mobility-shift assays using anti-SRE-binding protein (SREBP) and anti-NF-Ya antibodies confirmed that these SREs and the NF-Y site bind the respective factors. We also identified a second Sp-1 site located between nucleotides -40 and -30 that can substitute for the mutated Sp-1 site located between nucleotides -82 and -74. The reporter gene expression of the wild-type promoter and the Sp-1 site (nucleotides -82 to -74) mutant promoter was similar when SREBP1a [the N-terminal domain of SREBP (amino acids 1-520)] was constitutively overexpressed, suggesting that Sp-1 recruits SREBP to the SREs. Under the same conditions, an NF-Y site mutation resulted in significant loss of reporter gene expression, suggesting that NF-Y is required to activate the cholesterol response.  (+info)

Redirection of the respiro-fermentative flux distribution in Saccharomyces cerevisiae by overexpression of the transcription factor Hap4p. (8/416)

Reduction of aerobic fermentation on sugars by altering the fermentative/oxidative balance is of significant interest for optimization of industrial production of Saccharomyces cerevisiae. Glucose control of oxidative metabolism in baker's yeast is partly mediated through transcriptional regulation of the Hap4p subunit of the Hap2/3/4/5p transcriptional activator complex. To alleviate glucose repression of oxidative metabolism, we constructed a yeast strain with constitutively elevated levels of Hap4p. Genetic analysis of expression levels of glucose-repressed genes and analysis of respiratory capacity showed that Hap4p overexpression (partly) relieves glucose repression of respiration. Analysis of the physiological properties of the Hap4p overproducer in batch cultures in fermentors (aerobic, glucose excess) has shown that the metabolism of this strain is more oxidative than in the wild-type strain, resulting in a significant reduced ethanol production and improvement of growth rate and a 40% gain in biomass yield. Our results show that modification of one or more transcriptional regulators can be a powerful and a widely applicable tool for redirection of metabolic fluxes in microorganisms.  (+info)

CCAAT-Binding Factor (CBF) is a heteromeric transcription factor complex, primarily composed of three subunits - NFYA, NFYB, and NFYC, that binds to the CCAAT box DNA motif, involved in the regulation of various cellular processes including gene expression, differentiation, and development.

CCAAT-binding factor (CBF) is a transcription factor that binds to the CCAAT box, a specific DNA sequence found in the promoter regions of many genes. The CBF complex is composed of three subunits, NF-YA, NF-YB, and NF-YC, which are required for its DNA binding activity. The CBF complex plays important roles in various biological processes, including cell cycle regulation, differentiation, and stress response.

CCAAT-Enhancer-Binding Proteins (C/EBPs) are a family of transcription factors that bind to the CCAAT box motif in the promoter or enhancer regions of target genes, playing crucial roles in various biological processes including inflammation, immunity, differentiation, and metabolism.

CCAAT-Enhancer-Binding Proteins (C/EBPs) are a family of transcription factors that play crucial roles in the regulation of various biological processes, including cell growth, development, and differentiation. They bind to specific DNA sequences called CCAAT boxes, which are found in the promoter or enhancer regions of many genes.

The C/EBP family consists of several members, including C/EBPα, C/EBPβ, C/EBPγ, C/EBPδ, and C/EBPε. These proteins share a highly conserved basic region-leucine zipper (bZIP) domain, which is responsible for their DNA-binding and dimerization activities.

C/EBPs can form homodimers or heterodimers with other bZIP proteins, allowing them to regulate gene expression in a combinatorial manner. They are involved in the regulation of various physiological processes, such as inflammation, immune response, metabolism, and cell cycle control. Dysregulation of C/EBP function has been implicated in several diseases, including cancer, diabetes, and inflammatory disorders.

Core Binding Factors (CBFs) are a group of transcription factors, specifically CBFα and CBFβ, that form a complex and play crucial roles in hematopoiesis, cell cycle regulation, and chromatin remodeling by binding to specific DNA sequences, known as core binding sites, within the promoter regions of target genes.

Core binding factors (CBFs) are a group of proteins that play critical roles in the development and differentiation of hematopoietic cells, which are the cells responsible for the formation of blood and immune systems. The term "core binding factor" refers to the ability of these proteins to bind to specific DNA sequences, known as core binding sites, and regulate gene transcription.

The two main CBFs are:

1. Core Binding Factor Alpha (CBF-α): Also known as RUNX1 or AML1, this protein forms a complex with Core Binding Factor Beta (CBF-β) to regulate the expression of genes involved in hematopoiesis. Mutations in CBF-α have been associated with various types of leukemia and myelodysplastic syndromes.
2. Core Binding Factor Beta (CBF-β): Also known as PEBP2B, this protein partners with CBF-α to form the active transcription factor complex. CBF-β enhances the DNA binding affinity and stability of the CBF-α/CBF-β heterodimer.

In certain types of leukemia, chromosomal abnormalities can lead to the formation of fusion proteins involving CBF-α or CBF-β. These fusion proteins disrupt normal hematopoiesis and contribute to the development of cancer. Examples include the t(8;21) translocation that creates the AML1/ETO fusion protein in acute myeloid leukemia (AML) and the inv(16) inversion that forms the CBFB-MYH11 fusion protein in AML.

NFI (Nuclear Factor I) Transcription Factors are a family of transcriptional regulators involved in various cellular processes, including DNA replication, repair, and recombination, characterized by their ability to bind to the consensus sequence TTGGC(A/T)NNNNNGCCAA through a conserved NFI-specific DNA-binding domain.

Nuclear Factor I (NFI) transcription factors are a family of transcriptional regulatory proteins that bind to specific DNA sequences and play crucial roles in the regulation of gene expression. They are involved in various biological processes, including cell growth, differentiation, and development. NFI transcription factors recognize and bind to the consensus sequence TTGGC(N)5GCCAA, where N represents any nucleotide. In humans, there are four known members of the NFI family (NFIA, NFIB, NFIC, and NFIX), each with distinct expression patterns and functions. These factors can act as both activators and repressors of transcription, depending on the context and interacting proteins.

Y-Box-Binding Protein 1 (YB-1) is a multifunctional protein that binds to the Y-box DNA sequence element and plays critical roles in transcription, translation, DNA repair, and cellular stress responses, with its deregulation implicated in various human diseases including cancer.

Y-box-binding protein 1 (YB-1) is a multifunctional protein that belongs to the family of cold shock proteins. It binds to the Y-box DNA sequence, which is a cis-acting element found in the promoter regions of various genes. YB-1 plays a crucial role in several cellular processes such as transcription, translation, DNA repair, and nucleocytoplasmic shuttling.

YB-1 has been implicated in the regulation of gene expression in response to different stimuli, including stress, growth factors, and differentiation signals. It can function both as a transcriptional activator and repressor, depending on the cellular context and interacting partners. YB-1 is also involved in the regulation of mRNA stability, translation, and localization.

In addition to its role in normal cellular processes, YB-1 has been implicated in various pathological conditions, including cancer, neurodegenerative diseases, and viral infections. For instance, elevated levels of YB-1 have been found in several types of cancer, where it can promote tumor growth, invasion, and drug resistance.

Overall, YB-1 is a versatile protein that plays a critical role in the regulation of gene expression at multiple levels, and its dysregulation has been associated with various diseases.

'DNA-binding proteins' are defined as a category of proteins that interact with DNA, recognizing and binding to specific sequences or structures, thereby playing crucial roles in various cellular processes such as transcription regulation, DNA replication, repair, and recombination.

DNA-binding proteins are a type of protein that have the ability to bind to DNA (deoxyribonucleic acid), the genetic material of organisms. These proteins play crucial roles in various biological processes, such as regulation of gene expression, DNA replication, repair and recombination.

The binding of DNA-binding proteins to specific DNA sequences is mediated by non-covalent interactions, including electrostatic, hydrogen bonding, and van der Waals forces. The specificity of binding is determined by the recognition of particular nucleotide sequences or structural features of the DNA molecule.

DNA-binding proteins can be classified into several categories based on their structure and function, such as transcription factors, histones, and restriction enzymes. Transcription factors are a major class of DNA-binding proteins that regulate gene expression by binding to specific DNA sequences in the promoter region of genes and recruiting other proteins to modulate transcription. Histones are DNA-binding proteins that package DNA into nucleosomes, the basic unit of chromatin structure. Restriction enzymes are DNA-binding proteins that recognize and cleave specific DNA sequences, and are widely used in molecular biology research and biotechnology applications.

Transcription factors are specialized proteins that regulate the transcription of genetic information from DNA to RNA by binding to specific DNA sequences, thereby acting as molecular switches that turn genes on or off.

Transcription factors are proteins that play a crucial role in regulating gene expression by controlling the transcription of DNA to messenger RNA (mRNA). They function by binding to specific DNA sequences, known as response elements, located in the promoter region or enhancer regions of target genes. This binding can either activate or repress the initiation of transcription, depending on the properties and interactions of the particular transcription factor. Transcription factors often act as part of a complex network of regulatory proteins that determine the precise spatiotemporal patterns of gene expression during development, differentiation, and homeostasis in an organism.

In molecular biology, the "base sequence" refers to the specific order of nucleotides (adenine, thymine, guanine, and cytosine) that make up a DNA or RNA molecule, which is critical for encoding genetic information and determining the function of genes.

A base sequence in the context of molecular biology refers to the specific order of nucleotides in a DNA or RNA molecule. In DNA, these nucleotides are adenine (A), guanine (G), cytosine (C), and thymine (T). In RNA, uracil (U) takes the place of thymine. The base sequence contains genetic information that is transcribed into RNA and ultimately translated into proteins. It is the exact order of these bases that determines the genetic code and thus the function of the DNA or RNA molecule.

'Promoter regions, genetic' are specific DNA sequences located primarily upstream of a gene that serve as binding sites for RNA polymerase and transcription factors, thereby regulating the initiation and rate of transcription of that gene.

Promoter regions in genetics refer to specific DNA sequences located near the transcription start site of a gene. They serve as binding sites for RNA polymerase and various transcription factors that regulate the initiation of gene transcription. These regulatory elements help control the rate of transcription and, therefore, the level of gene expression. Promoter regions can be composed of different types of sequences, such as the TATA box and CAAT box, and their organization and composition can vary between different genes and species.

'Molecular sequence data' refers to the specific arrangement of molecules, such as nucleotides in DNA or amino acids in proteins, that are represented in a linear fashion and used to identify, compare, and analyze biological characteristics or relationships among organisms at the molecular level.

Molecular sequence data refers to the specific arrangement of molecules, most commonly nucleotides in DNA or RNA, or amino acids in proteins, that make up a biological macromolecule. This data is generated through laboratory techniques such as sequencing, and provides information about the exact order of the constituent molecules. This data is crucial in various fields of biology, including genetics, evolution, and molecular biology, allowing for comparisons between different organisms, identification of genetic variations, and studies of gene function and regulation.

Core Binding Factor beta Subunit (CBFβ) is a non-DNA binding regulatory subunit that heterodimerizes with the alpha subunit of Core Binding Factor (CBF), forming a transcription factor complex that recognizes and binds to specific DNA sequences, playing crucial roles in hematopoiesis, neurogenesis, and limb development.

Core Binding Factor-beta (CBF-β) is a subunit of the Core Binding Factor (CBF), which is a heterodimeric transcription factor composed of a DNA-binding alpha subunit and a non-DNA binding beta subunit. The CBF plays a crucial role in hematopoiesis, the process of blood cell development, by regulating the expression of various genes involved in this process.

The CBF-β subunit is a 36 kDa protein that is encoded by the CBFB gene in humans. It does not bind to DNA directly but instead forms a complex with the DNA-binding alpha subunit, which is either RUNX1 (also known as AML1), RUNX2, or RUNX3. The CBF-β subunit stabilizes the interaction between the alpha subunit and DNA, enhances its DNA-binding affinity, and increases the transcriptional activity of the complex.

Mutations in the CBFB gene have been associated with several hematological disorders, including acute myeloid leukemia (AML), myelodysplastic syndromes (MDS), and familial platelet disorder with predisposition to AML (FPD/AML). These mutations can lead to aberrant transcriptional regulation of hematopoietic genes, resulting in the development of these disorders.

POL1 (Polymerase Alpha) Transcription Initiation Complex Proteins are a set of proteins, including POL1, that come together to form the initiation complex for the transcription of DNA into RNA, primarily at the start of gene expression in eukaryotic cells.

POL1 (Polymerase 1) Transcription Initiation Complex Proteins are a set of proteins that come together to form the initiation complex for the transcription of ribosomal RNA (rRNA) genes in eukaryotic cells. The POL1 complex includes RNA polymerase I, select transcription factors, and other regulatory proteins. This complex is responsible for the transcription of rRNA genes located within the nucleolus, a specialized region within the cell nucleus. Proper assembly and functioning of this initiation complex are crucial for the production of ribosomes, which play a critical role in protein synthesis.

Core Binding Factor (CBF) alpha subunits, also known as RUNX proteins, are transcription factors that form heterodimers with CBF beta subunits and play critical roles in hematopoiesis, neurogenesis, and skeletal development by regulating the expression of target genes through binding to specific DNA sequences.

Core Binding Factor (CBF) is a transcription factor that plays a crucial role in the development and differentiation of various tissues, including hematopoietic cells. It is composed of two subunits: alpha (CBFA or AML1) and beta (CBFB or PEBP2b).

The CBFA subunit, also known as RUNX1, is a transcription factor that binds to DNA and regulates the expression of target genes involved in hematopoiesis, neurogenesis, and other developmental processes. It contains a highly conserved DNA-binding domain called the runt homology domain (RHD) that recognizes specific DNA sequences.

Mutations in CBFA have been associated with various hematological disorders, including acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), and familial platelet disorder with predisposition to AML (FDPA). These mutations can lead to altered gene expression, impaired differentiation, and increased proliferation of hematopoietic cells, contributing to the development of these diseases.

Telomeric Repeat Binding Protein 1 (TRF1) is a key component of the shelterin complex, which binds and protects telomeres, playing a crucial role in maintaining telomere length, structure, and function, and thus contributing to genomic stability.

Telomeric Repeat Binding Protein 1 (TRF1) is a protein that binds to the telomeres, which are the repetitive DNA sequences found at the ends of chromosomes. TRF1 plays a crucial role in the protection and regulation of telomere length. It helps to form a protective cap on the end of the chromosome, preventing it from being recognized as damaged or broken. Additionally, TRF1 is involved in the negative regulation of telomerase, an enzyme that adds repetitive DNA sequences to the ends of chromosomes, thereby controlling the length of the telomeres. Mutations in TRF1 have been associated with certain types of cancer and premature aging disorders.

G-box Binding Factors are transcription factors, specifically the basic leucine zipper (bZIP) family members, that bind to the G-box DNA motif (CACGTG) in the promoter regions of genes to regulate their expression, primarily involved in plant growth, development, and stress responses.

I'm sorry for any confusion, but "G-Box Binding Factors" is not a widely recognized or established term in medical or molecular biology literature. The "G-box" is a specific sequence of DNA that can be found in the promoter regions of many genes and serves as a binding site for various transcription factors. Transcription factors are proteins that regulate gene expression by binding to specific DNA sequences and either promoting or inhibiting the initiation of transcription.

However, "G-Box Binding Factors" is too broad since multiple transcription factors can bind to the G-box sequence. Some examples of transcription factors known to bind to the G-box include proteins like GBF (G-box binding factor), HSF (heat shock transcription factor), and bZIP (basic region/leucine zipper) proteins, among others.

If you have a more specific context or reference related to "G-Box Binding Factors," I would be happy to help provide further information based on that context.

CEBP-β, also known as C/EBP beta, is a transcription factor protein that binds to the CCAAT box motif in the enhancer regions of target genes, playing crucial roles in regulation of immune response, cell differentiation, and metabolism, among other biological processes.

CCAAT-Enhancer-Binding Protein-beta (CEBPB) is a transcription factor that plays a crucial role in the regulation of gene expression. It binds to the CCAAT box, a specific DNA sequence found in the promoter or enhancer regions of many genes. CEBPB is involved in various biological processes such as cell growth, development, and immune response. Dysregulation of CEBPB has been implicated in several diseases, including cancer and inflammatory disorders.

Transcription, Genetic is the process of creating an RNA copy of a sequence of DNA through the action of RNA polymerase and various transcription factors, which serves as a template for protein synthesis or other cellular functions.

Genetic transcription is the process by which the information in a strand of DNA is used to create a complementary RNA molecule. This process is the first step in gene expression, where the genetic code in DNA is converted into a form that can be used to produce proteins or functional RNAs.

During transcription, an enzyme called RNA polymerase binds to the DNA template strand and reads the sequence of nucleotide bases. As it moves along the template, it adds complementary RNA nucleotides to the growing RNA chain, creating a single-stranded RNA molecule that is complementary to the DNA template strand. Once transcription is complete, the RNA molecule may undergo further processing before it can be translated into protein or perform its functional role in the cell.

Transcription can be either "constitutive" or "regulated." Constitutive transcription occurs at a relatively constant rate and produces essential proteins that are required for basic cellular functions. Regulated transcription, on the other hand, is subject to control by various intracellular and extracellular signals, allowing cells to respond to changing environmental conditions or developmental cues.

Core Binding Factor Alpha 2 Subunit is a protein that heterodimerizes with Core Binding Factor Beta to form the transcription factor complex, CBFA2/RUNX1, which plays critical roles in hematopoiesis and is associated with certain leukemias when mutated.

Core Binding Factor Alpha 2 Subunit, also known as CBF-A2 or CEBP-α, is a protein that forms a complex with other proteins to act as a transcription factor. Transcription factors are proteins that help regulate the expression of genes by binding to specific DNA sequences and controlling the rate of transcription of genetic information from DNA to RNA.

CBF-A2 is a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors, which are important in regulating various biological processes such as cell growth, development, and inflammation. CBF-A2 forms a heterodimer with Core Binding Factor Beta (CBF-β) to form the active transcription factor complex known as the core binding factor (CBF).

The CBF complex binds to the CCAAT box, a specific DNA sequence found in the promoter regions of many genes. By binding to this sequence, the CBF complex can either activate or repress the transcription of target genes, depending on the context and the presence of other regulatory factors.

Mutations in the gene encoding CBF-A2 have been associated with several human diseases, including acute myeloid leukemia (AML) and multiple myeloma. In AML, mutations in the CBF-A2 gene can lead to the formation of abnormal CBF complexes that disrupt normal gene expression patterns and contribute to the development of leukemia.

CCAAT-binding factor complex | SGDCCAAT-binding factor complex | SGD
CCAAT-binding factor complex. GO ID. GO:0016602 Aspect. Cellular Component. Description. A heteromeric transcription factor ... CBF complex, NF-Y transcription factor complex, nuclear transcription factor Y complex View GO Annotations in other species in ... complex that binds to the CCAAT-box upstream of promoters; functions as both an activator and a repressor, depending on its ...
https://www.yeastgenome.org/go/GO:0016602
CCAAT/enhancer-binding protein β promotes receptor activator of nuclear factor-kappa-B ligand (RANKL) expression and osteoclast...CCAAT/enhancer-binding protein β promotes receptor activator of nuclear factor-kappa-B ligand (RANKL) expression and osteoclast...
ChIP and IP assays revealed the cooperative binding of C/EBPβ and ATF4 on the RANKL promoter. We demonstrated that C/EBPβ, ... In this study, we examined whether C/EBPβ mediates the expression of receptor activator of nuclear factor-kappa-B ligand (RANKL ... The cooperation of C/EBPβ and activation transcription factor-4 (ATF4) in the regulation of the RANKL promoter was also ... is a transcription factor that is activated in the synovium in rheumatoid arthritis (RA) and promotes expression of various ...
https://arthritis-research.biomedcentral.com/articles/10.1186/s13075-015-0532-6/figures/1
MedlinePlus: Genes: CMedlinePlus: Genes: C
CDT1: chromatin licensing and DNA replication factor 1. *CEBPA: CCAAT enhancer binding protein alpha ...
https://medlineplus.gov/genetics/gene-c/
Introductory Chapter: Gene Expression Controlling System and Its Application to Medical Sciences | IntechOpenIntroductory Chapter: Gene Expression Controlling System and Its Application to Medical Sciences | IntechOpen
2.2.3. High-mobility group (HMG) proteins and heteromeric CCAAT-binding factors. This group includes SOX [17] and NF-Y [18] ... Water-insoluble factors, steroids, and vitamins will easily go through lipid bilayer to bind to nuclear receptors. On the other ... 2. Transcription factors (TFs) in mammalian cells. Transcription factors are divided into two groups. First, the general TFs ( ... Nucleotide excision repair is impaired by binding of transcription factors to DNA. Nature. 2016;. 532. (7598):264-267. ...
https://www.intechopen.com/chapters/63319
CCAAT-enhancer-binding proteins - WikipediaCCAAT-enhancer-binding proteins - Wikipedia
CCAAT-enhancer-binding proteins (or C/EBPs) is a family of transcription factors composed of six members, named from C/EBPα to ... "Structural basis for DNA recognition by the basic region leucine zipper transcription factor CCAAT/enhancer-binding protein ... "CCAAT/enhancer-binding protein family members recruit the coactivator CREB-binding protein and trigger its phosphorylation". ... The function of CCAAT/enhancer-binding proteins in cancer is thus clearly context dependent but largely tumor suppressive. C/ ...
https://en.wikipedia.org/wiki/CCAAT-enhancer-binding_proteins
Frontiers | Iron - A Key Nexus in the Virulence of Aspergillus fumigatusFrontiers | Iron - A Key Nexus in the Virulence of Aspergillus fumigatus
... the GATA-factor SreA and the bZip-factor HapX. During iron sufficiency, SreA represses iron uptake, including reductive iron ... Recently, the sterol regulatory element binding protein SrbA was found to be essential for adaptation to iron starvation, ... the GATA-factor SreA and the bZip-factor HapX. During iron sufficiency SreA represses iron uptake, including reductive iron ... Recently, the sterol-regulatory element-binding protein SrbA was found to be essential for adaptation to iron starvation, ...
https://www.frontiersin.org/articles/10.3389/fmicb.2012.00028/full
NFIX - WikipediaNFIX - Wikipedia
"Entrez Gene: NFIX nuclear factor I/X (CCAAT-binding transcription factor)". Singh SK, Bhardwaj R, Wilczynska KM, Dumur CI, ... gene expression is mediated by decreased DNA binding of nuclear factor I proteins which control constitutive TTF-1 expression ... for the human transcription factor nuclear factor I by FISH". Genomics. 28 (1): 66-73. doi:10.1006/geno.1995.1107. PMID 7590749 ... Nuclear factor 1 X-type is a protein that in humans is encoded by the NFIX gene. NFI-X3, a splice variant of NFIX, regulates ...
https://en.wikipedia.org/wiki/NFIX
The aged epidermal permeability barrier: basis for functional abnormalitiesThe aged epidermal permeability barrier: basis for functional abnormalities
Various cytokine/growth factor signaling pathways are a … ... CCAAT-Enhancer-Binding Proteins / physiology * Cell Membrane ... Various cytokine/growth factor signaling pathways are abnormal in aged skin, particularly in the interleukin-1 family. Barrier ...
https://pubmed.ncbi.nlm.nih.gov/11913735/
HIF-P4H-2 inhibition enhances intestinal fructose metabolism and induces thermogenesis protecting against NAFLD | Journal of...HIF-P4H-2 inhibition enhances intestinal fructose metabolism and induces thermogenesis protecting against NAFLD | Journal of...
CCAAT-enhancer-binding protein alpha; CHREBP, carbohydrate-responsive element-binding protein; DGAT1, diacylglycerol o- ... sterol regulatory element-binding protein 1c; UCP-1, uncoupling protein 1; VEGFA, vascular endothelial growth factor A; WAT, ... ACSL1, acyl-CoA synthetase long-chain family member 1; AUC, area under the curve; CEBPA, CCAAT-enhancer-binding protein alpha; ... sterol regulatory element-binding protein 1c; TG, triglycerides; TGFb1, transforming growth factor beta 1 ...
https://link.springer.com/article/10.1007/s00109-020-01903-0
Host cellular microRNA involvement in the control of hepatitis B virus gene expression and replicationHost cellular microRNA involvement in the control of hepatitis B virus gene expression and replication
CCAAT/enhancer binding protein beta; HCC: Hepatocellular carcinoma; HNF1α: Human hepatocyte nuclear factor 1 α. ... it has been reported that CCAAT/enhancer binding protein beta, which can bind to HBV promoters and enhancers, is one target of ... a liver-enriched transcription factor activated by bile acids. FXRα can function as a transcription factor by binding to the ... Shaul Y, Ben-Levy R, De-Medina T. High affinity binding site for nuclear factor I next to the hepatitis B virus S gene promoter ...
https://wjgnet.com/1948-5182/full/v7/i4/696.htm
Function of C/EBPδ in a regulatory circuit that discriminates between transient and persistent TLR4-induced signals | Nature...Function of C/EBPδ in a regulatory circuit that discriminates between transient and persistent TLR4-induced signals | Nature...
... to predict and confirm the existence of a gene-regulatory network involving dynamic interaction among the transcription factors ... Aderem and colleagues use systems-biology approaches to show that three transcription factors, NF-κB, ATF3 and C/EBPδ, work ... Lekstrom-Himes, J. & Xanthopoulos, K.G. Biological role of the CCAAT/enhancer-binding protein family of transcription factors. ... CCAAT/enhancer-binding protein family members recruit the coactivator CREB-binding protein and trigger its phosphorylation. J. ...
https://www.nature.com/articles/ni.1721?error=cookies_not_supported&code=1d4015fc-a71f-40eb-8761-03d5f8da78bd
Effects of GSK2606414 on cell proliferation and endoplasmic reticulum stress‑associated gene expression in retinal pigment...Effects of GSK2606414 on cell proliferation and endoplasmic reticulum stress‑associated gene expression in retinal pigment...
ATF4, activating transcription factor 4; CHOP, CCAAT/enhancer-binding protein homologous protein; VEGF, vascular endothelial ... ATF4 induces expression of the proapoptotic transcription factor CCAAT/enhancer-binding protein homologous protein (CHOP), ... CCAAT/enhancer‑binding protein homologous protein (CHOP) and vascular endothelial growth factor (VEGF) in human retinal pigment ... Cells were subsequently washed in 1X binding buffer and resuspended in 200 µl binding buffer, followed by the addition of 5 µl ...
https://www.spandidos-publications.com/10.3892/mmr.2017.6418
Epidermodysplasia Verruciformis: Background, Pathophysiology, EtiologyEpidermodysplasia Verruciformis: Background, Pathophysiology, Etiology
The E6 protein targets the transcription factor CCAAT/enhancer-binding protein (C/EBP)α, which is a tumor suppressor of UV- ... such as tumor necrosis factor-alpha (TNF-a), transforming growth factor-beta (TGF-b), interleukin 4, and interleukin 10, as ... 19, 20, 21, 22] This gene encodes calcium and integrin-binding protein 1, which forms a complex with EVER1 and EVER2. [20] The ... ZnT-1/EVER has been found to bind the E5 protein, resulting in increased concentrations of free zinc in keratinocytes. In non- ...
https://emedicine.medscape.com/article/1131981-overview
Selective inhibition of cytokine-activated extracellular signal-regulated kinase by cyclic amp via Epac1-dependent induction of...Selective inhibition of cytokine-activated extracellular signal-regulated kinase by cyclic amp via Epac1-dependent induction of...
CCAAT/enhancer binding protein; JAK, Janus kinase; MEK, mitogen-activated protein/ERK kinase; SHP-2, SH2 domain-containing ... EPAC1 and ERK-dependent activation of C/EBP transcription factors: a new cyclic AMP-activated anti-inflammatory gene expression ... and subsequent phosphorylation of transcription factor C/EBPβ, both of which were resistant to PKA inhibition. However, siRNA ... cAMP response element binding protein; MEF, murine embryonic fibroblast ...
https://eprints.gla.ac.uk/7622/
Research AG LeuztzResearch AG Leuztz
The lab currently focuses on functions of transcription factors of the CCAAT Enhancer Binding Protein family (C/EBPs). We use ... Currently, we concentrate on the functions of "pioneering transcription factors" CCAAT Enhancer Binding Proteins alpha and beta ... PRISMA and BioID disclose a motifs-based interactome of the intrinsically disordered transcription factor C/EBPα *E. Ramberger ... Carm1-arginine methylation of the transcription factor C/EBPα regulates transdifferentiation velocity *G. Torcal Garcia ...
https://www.mdc-berlin.de/leutz
IJMS | Free Full-Text | Predicting FOXM1-Mediated Gene Regulation through the Analysis of Genome-Wide FOXM1 Binding Sites in...IJMS | Free Full-Text | Predicting FOXM1-Mediated Gene Regulation through the Analysis of Genome-Wide FOXM1 Binding Sites in...
Consistent with previous studies, different TF binding motifs were identified at FOXM1 binding sites, while the NFY binding ... In this study, a comprehensive meta-analysis of genome-wide FOXM1 binding sites in ECC-1, GM12878, K562, MCF-7, and SK-N-SH ... motif was found at 81% of common FOXM1 binding sites in promoters of cell cycle-related genes. The results indicated that FOXM1 ... is a key transcription factor (TF) that regulates a common set of genes related to the cell cycle in various cell types. ...
https://www.mdpi.com/1422-0067/21/17/6141
GADD153/CHOP Overexpression Lysate (NBL1-09772): Novus BiologicalsGADD153/CHOP Overexpression Lysate (NBL1-09772): Novus Biologicals
GADD153 encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions ... and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by ...
https://www.novusbio.com/products/gadd153-chop-lysate_nbl1-09772
Non-cyp51A Azole-Resistant Aspergillus fumigatus Isolates with Mutation in HMG-CoA Reductase - Volume 24, Number 10-October...
A recent study showed that HapE forms a CCAAT binding complex, which plays a role in the negative regulation of cyp51A ... Hagiwara D, Miura D, Shimizu K, Paul S, Ohba A, Gonoi T, et al. A novel zn2-cys6 transcription factor atrr plays a key role in ... Sterol biosynthesis and azole tolerance is governed by the opposing actions of SrbA and the CCAAT binding complex. PLoS Pathog ... Polyene drugs such as amphotericin B and nystatin bind to ergosterol in the plasma membrane, leading to cell death by promoting ...
https://wwwnc.cdc.gov/eid/article/24/10/18-0730
Cells | Free Full-Text | Shared and Divergent Epigenetic Mechanisms in Cachexia and SarcopeniaCells | Free Full-Text | Shared and Divergent Epigenetic Mechanisms in Cachexia and Sarcopenia
Similarly, the constant expression of CCAAT/enhancer binding protein (C/EBPβ), a transcription factor that positively regulates ... The region where HuR binds is near the binding region of miR-330. Consequently, the binding of HuR to STAT3 inhibits the ... Marchildon, F.; Fu, D.; Lala-Tabbert, N.; Wiper-Bergeron, N. CCAAT/enhancer binding protein beta protects muscle satellite ... Several factors remain the main indicators of sarcopenia pathophysiology. These factors include declines in anabolic hormone ...
https://www.mdpi.com/2073-4409/11/15/2293
High Mobility Group B Proteins, Their Partners, and Other Redox Sensors in Ovarian and Prostate CancerHigh Mobility Group B Proteins, Their Partners, and Other Redox Sensors in Ovarian and Prostate Cancer
... encoding a transcription factor that binds to antioxidant response elements (AREs) and that is regarded as a promising ... D. Dolfini and R. Mantovani, "Targeting the Y/CCAAT box in cancer: YB-1 (YBX1) or NF-Y," Cell Death and Differentiation, vol. ... HMGB proteins are able to bind to other nuclear proteins that do not have DNA binding capacity but that have a role in ... β DNA binding domain in DNA binding and interaction with HMGB," The Journal of Biological Chemistry, vol. 279, no. 15, pp. ...
https://www.hindawi.com/journals/omcl/2016/5845061/
Cyclic AMP Responsive Element Binding Proteins Are Involved in Emergency Granulopoiesis through the Upregulation of CCAAT...Cyclic AMP Responsive Element Binding Proteins Are Involved in 'Emergency' Granulopoiesis through the Upregulation of CCAAT...
In contrast to the definitive role of the transcription factor, CCAAT/Enhancer binding protein α (C/EBPα), in steady-state ... Cyclic AMP Responsive Element Binding Proteins Are Involved in Emergency Granulopoiesis through the Upregulation of CCAAT/ ... Cyclic AMP responsive element binding proteins are involved in emergency granulopoiesis through the upregulation of CCAAT/ ... CREB was upregulated, phosphorylated and bound to the CREs in response to GM-CSF stimulation. Retroviral transduction of a ...
https://dash.harvard.edu/handle/1/11180377
Comparative molecular analysis of early and late cancer cachexia-induced muscle wasting in mouse modelsComparative molecular analysis of early and late cancer cachexia-induced muscle wasting in mouse models
CCAAT/enhancer binding protein beta (C/EBPβ) is an important transcription factor involved in cellular metabolism and ... Ramji DP and Foka P: CCAAT/enhancer-binding proteins: Structure, function and regulation. Biochem J. 365:561-575. 2002. View ... Lokireddy S, Wijesoma IW, Bonala S, Wei M, Sze SK, McFarlane C, Kambadur R and Sharma M: Myostatin is a novel tumoral factor ... Penner G, Gang G, Sun X, Wray C and Hasselgren PO: C/EBP DNA-binding activity is upregulated by a glucocorticoid-dependent ...
https://www.spandidos-publications.com/10.3892/or.2016.5165?text=fulltext
MyeloCult™ - Long-Term Culture-Initiating Cell (LTC-IC) AssaysMyeloCult™ - Long-Term Culture-Initiating Cell (LTC-IC) Assays
2006) CCAAT/enhancer binding proteins alpha and epsilon cooperate with all-trans retinoic acid in therapy but differ in their ... 2006) The ETS factor TEL2 is a hematopoietic oncoprotein. Blood 107(3): 1124-1132. Carmen M et al. (2004) Interleukin-6 ... 2003) CCAAT/Enhancer binding proteins repress the leukemic phenotype of acute myeloid leukemia. Blood 101(3): 1141-1148. Jin H ... 2006) Granulocyte colony-stimulating factor preferentially stimulates proliferation of monosomy 7 cells bearing the isoform IV ...
https://www.stemcell.com/products/popular-brands/myeloculttm.html
3,3′‐Diindolylmethane Enhances Glucose Uptake Through Activation of Insulin Signaling in 3T3‐L1 Adipocytes | Request PDF3,3′‐Diindolylmethane Enhances Glucose Uptake Through Activation of Insulin Signaling in 3T3‐L1 Adipocytes | Request PDF
CCAAT-/enhancer-binding protein α, sterol regulatory element-binding transcription factor 1c and fatty acid synthase. ... Inhibition of early differentiation was reflected by down-regulation of early adipogenic factors such as CCAAT-enhancer binding ... Nuclear factor E2-related factor 2 (Nrf2) is a cap-n-collar basic leucine zipper (CNC-bZIP) transcription factor that is well ... The growth factors fibroblast growth factor, platelet-derived growth factor, and bombesin did not substitute for insulin during ...
https://www.researchgate.net/publication/324926095_33
CDP/Cutl1 controls differentiation-specific MMTV and cellular gene expression in the mammary glandCDP/Cutl1 controls differentiation-specific MMTV and cellular gene expression in the mammary gland
Virgin mammary glands have high levels of full-length CDP that binds to negative regulatory elements (NRE) to repress MMTV ... CCAAT displacement protein (CDP), is a transcriptional repressor of MMTV. ... Furthermore, the loss of DNA-binding activity correlates with increased expression of MMTV and other mammary-specific genes, ... During late pregnancy, CDP DNA-binding activity declines concomitant with an increase in viral transcripts. This study ...
https://repositories.lib.utexas.edu/items/0d154e67-52b0-4e88-b1a8-e418c01fd696
JCI - Chop deletion reduces oxidative stress, improves β cell function, and promotes cell survival in multiple mouse models of...
... cAMP-responsive-element-binding protein (CREB) interact with the CCAAT/enhancer-binding protein (C/EBP)-ATF composite site to ... mRNA to produce a potent transcription factor (17). ATF6 is a basic leucine zipper-containing transcription factor that, upon ... Role of C/EBP homologous protein (CHOP-10) in the programmed activation of CCAAT/enhancer-binding protein-beta during ... and the transcription factor activating transcription factor 6 (ATF6). This response collectively halts initiation of ...
https://www.jci.org/articles/view/34587
Cross Talk of Proteostasis and Mitostasis in Cellular Homeodynamics, Ageing, and DiseaseCross Talk of Proteostasis and Mitostasis in Cellular Homeodynamics, Ageing, and Disease
... cAMP-responsive-element-binding protein (CREB) interact with the CCAAT/enhancer-binding protein (C/EBP)-ATF composite site to ... the nuclear factor-kappa beta (NF-κB), and the Hypoxia Inducible Factor 1α (HIF1α). The transcription factors NRF1 and NRF2 ... c-Myc induces the activation of the PGC-1β factor; on the other hand, mitochondrial biogenesis is inhibited when HIF1 factors ... Another downstream component of HAF-1 is the bZip transcription factor ATFS-1 (Activating Transcription Factor associated with ...
https://www.hindawi.com/journals/omcl/2016/4587691/
Leonardo Aliaga | Stanford MedicineLeonardo Aliaga | Stanford Medicine
... reticulum stress and unfolded protein response and the resulting up-regulation of pro-apoptotic factor CCAAT/enhancer-binding ... reticulum stress and unfolded protein response and the resulting up-regulation of pro-apoptotic factor CCAAT/enhancer-binding ... reticulum stress and unfolded protein response and the resulting up-regulation of pro-apoptotic factor CCAAT/enhancer-binding ... to a consecutive series of CM1 patients to identify specific factors or symptoms that predispose to a better or worse surgical ...
https://med.stanford.edu/profiles/leonardo-aliaga
Negative selection maintains transcription factor binding motifs in human cancer | BMC Genomics | Full TextNegative selection maintains transcription factor binding motifs in human cancer | BMC Genomics | Full Text
In particular, mutations in DNA binding sites recognized by transcription factors can alter regulator binding affinities and, ... Here we present analysis of transcription factor binding motifs co-localized with non-coding variants. To avoid statistical ... Cancer somatic mutations in binding sites of selected transcription factors have been found under positive selection. However, ... Further analysis of transcription factors with conserved binding motifs can reveal cell regulatory pathways crucial for the ...
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-2728-9
Immune cell - SERPINE1 - The Human Protein AtlasImmune cell - SERPINE1 - The Human Protein Atlas
CCAAT enhancer binding protein zeta. 0.9048. 15. LRIF1. Ligand dependent nuclear receptor interacting factor 1. 0.9048. 14. ...
http://www.proteinatlas.org/ENSG00000106366-SERPINE1/immune+cell
Transcription factorsProteinsEnhancer binding protePeroxisome proliferHomologous proteinCHOPSterol regulatEBPsGeneRegulatoryInduced plurActivatorGranulocyte-macropCellularPathwaysForkheadEukaryoticGenesDifferentiationPhosphorylationCREBAffinityTranscriptsCytokineGlucoseRegionsLigandInflammatoryMolecularSitesMotifsGrowthInitiationRoleCassetteExpression
Transcription factors27
  • Recent global analyses of gene transcripts revealed that specific transcription factors (TFs) and their networking systems physiologically correspond to the onset of human diseases, including cancer. (intechopen.com)
  • Transcription factors are divided into two groups. (intechopen.com)
  • CCAAT-enhancer-binding proteins (or C/EBPs) is a family of transcription factors composed of six members, named from C/EBPα to C/EBPζ. (wikipedia.org)
  • Once bound to DNA, C/EBPs can recruit so-called co-activators (such as CBP) that in turn can open up chromatin structure or recruit basal transcription factors. (wikipedia.org)
  • This domain is involved in dimerization and DNA binding, as are other transcription factors of the leucine zipper domain-containing family (c-Fos and c-jun). (wikipedia.org)
  • Members of the C/EBP family can form homodimers or heterodimers with other C/EBPs and with other transcription factors, which may or may not contain the leucine zipper domain. (wikipedia.org)
  • C/EBPβ and δ promote adipogenesis, at least in part by inducing the expression of the "master" adipogenic transcription factors C/EBPα and PPARγ. (wikipedia.org)
  • In Aspergillus fumigatus , iron starvation causes extensive transcriptional remodeling involving two central transcription factors, which are interconnected in a negative transcriptional feed-back loop: the GATA-factor SreA and the bZip-factor HapX. (frontiersin.org)
  • Here we use systems-biology approaches to predict and confirm the existence of a gene-regulatory network involving dynamic interaction among the transcription factors NF-κB, C/EBPδ and ATF3 that controls inflammatory responses. (nature.com)
  • Currently, we concentrate on the functions of "pioneering transcription factors" CCAAT Enhancer Binding Proteins alpha and beta ( C/EBPα,β ) in development and disease. (mdc-berlin.de)
  • Hematopoietic transcription factors (= gene regulatory proteins) concertedly control cell proliferation, lineage commitment, and cell differentiation. (mdc-berlin.de)
  • Mutations in these key transcription factors dysregulate hematopoiesis and cause diseases, such as leukemia and immune defects. (mdc-berlin.de)
  • The lab currently focuses on functions of transcription factors of the CCAAT Enhancer Binding Protein family (C/EBPs). (mdc-berlin.de)
  • GADD153 encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. (novusbio.com)
  • In particular, mutations in DNA binding sites recognized by transcription factors can alter regulator binding affinities and, consequently, expression of target genes. (biomedcentral.com)
  • Cancer somatic mutations in binding sites of selected transcription factors have been found under positive selection. (biomedcentral.com)
  • For many transcription factors, including multiple members of FOX, HOX, and NR families, we show that human cancers accumulate fewer mutations than expected by chance that increase or decrease affinity of predicted binding sites. (biomedcentral.com)
  • Further analysis of transcription factors with conserved binding motifs can reveal cell regulatory pathways crucial for the survivability of various human cancers. (biomedcentral.com)
  • RNA-seq results showed that transcription factors and macrophage inflammatory proteins were significantly downregulated in the DNMT3A mutant clones. (frontiersin.org)
  • Our results suggest that Foxo3a transcription factors may be involved in the pathogenesis of periapical granulomas. (go.jp)
  • Functional genomics data indicate that 2 of the suggestive SNPs (rs2446823 and rs2446824) are located within putative binding sites for the CCAAT/Enhancer Binding Protein (CEBP) and Hepatocyte Nuclear Factor 4, Alpha transcription factors (TFs), respectively. (cdc.gov)
  • CCAAT/enhancer binding proteins (C/EBPs) are a family of transcription factors that regulate cellular growth and differentiation, metabolism, and inflammation [ 18 ]. (biomedcentral.com)
  • CCAAT/enhancer binding proteins (C/EBPs) are a family of leucine zipper, transcription factors that bind to DNA as homodimers and heterodimers. (johnshopkins.edu)
  • In addition, transcription prediction showed that mutation of these 6 SNPs might alternate the binding of particular transcription factors. (biomedcentral.com)
  • This divergence may origin from the differential effect of inotilone on transcription factors, nuclear factor κB (NF κB) and CCAAT enhancer-binding protein (C/EBP). (rutgers.edu)
  • Furthermore, this resource has allowed the identification of cis -regulatory sites in previously unexplored regions of the genome and the cooperating transcription factors underlying estrogen signaling in breast cancer. (nature.com)
  • Figure 5: Involvement of cooperating transcription factors at estrogen receptor binding sites. (nature.com)
Proteins5
  • The others are the site-specific TFs or the DNA sequence-specific binding proteins. (intechopen.com)
  • C/EBP proteins interact with the CCAAT (cytosine-cytosine-adenosine-adenosine-thymidine) box motif, which is present in several gene promoters. (wikipedia.org)
  • In addition, the transcripts of CRE binding (CREB) family proteins were readily detected in hematopoietic stem/progenitor cells. (harvard.edu)
  • Zahnow, CA 2002, ' CCAAT/enhancer binding proteins in normal mammary development and breast cancer ', Breast Cancer Research , vol. 4, no. 3, pp. 113-121. (johnshopkins.edu)
  • Molecular docking analysis revealed that the small molecular components of LZP can bind spontaneously to crucial proteins involved in the IL-17 and TNF signaling pathways, including MAPK15, MMP3, VCAM1, and CASP3. (bvsalud.org)
Enhancer binding prote2
  • Scholars@Duke publication: A 30-kDa alternative translation product of the CCAAT/enhancer binding protein alpha message: transcriptional activator lacking antimitotic activity. (duke.edu)
  • Full-length (42 kDa) CCAAT/enhancer binding protein alpha (C/EBP alpha) (p42) has been implicated in the transcriptional activation of adipocyte genes including the 422(aP2) and C/EBP alpha genes during differentiation of 3T3-L1 preadipocytes. (duke.edu)
Peroxisome prolifer2
  • Results DIM, but not I3C, increased adipocyte differentiation through upregulation of peroxisome proliferator‐activated receptor γ and CCAAT/enhancer‐binding protein α. (researchgate.net)
  • Additionally, TME concentrations ranging from 20 µg/mL to 500 µg/mL led to a decrease in the expression of adipocyte differentiation regulators, peroxisome proliferator-activated receptor γ, CCAAT element binding protein α, and sterol regulatory element binding transcription factor 1. (foodandnutritionresearch.net)
Homologous protein4
  • The present study investigated the effects of GSK2606414 on proliferation, apoptosis, and the expression of activating transcription factor 4 (ATF4), CCAAT/enhancer‑binding protein homologous protein (CHOP) and vascular endothelial growth factor (VEGF) in human retinal pigment epithelial (RPE) cells under endoplasmic reticulum (ER) stress. (spandidos-publications.com)
  • ATF4 induces expression of the proapoptotic transcription factor CCAAT/enhancer-binding protein homologous protein (CHOP), which mediates PERK-induced apoptosis ( 16 ). (spandidos-publications.com)
  • Chronic ER stress promotes apoptosis, at least in part through the UPR-induced transcription factor C/EBP homologous protein (CHOP). (jci.org)
  • TGZ elevated DR5 expression at the promoter level through the CCAAT/enhancer-binding protein homologous protein (CHOP) binding site. (nih.gov)
CHOP3
  • These findings suggest that CHOP is a fundamental factor that links protein misfolding in the ER to oxidative stress and apoptosis in β cells under conditions of increased insulin demand. (jci.org)
  • The transcription factor CHOP (CCAAT/enhancer-binding protein homology protein) accumulated in response to carfilzomib exposure, with CHOP depletion conferring protection against cytotoxicity. (ascopost.com)
  • We have used Southern blot technique to test whether a gene of the CCAAT/enhancer binding protein (C/EBP) family, CHOP, which maps to 12q13 and is assumed to be involved in adipocyte differentiation, could be the 12q gene in question. (lu.se)
Sterol regulat1
  • Recently, the sterol regulatory element binding protein SrbA was found to be essential for adaptation to iron starvation, thereby linking regulation of iron metabolism, ergosterol biosynthesis, azole drug resistance, and hypoxia adaptation. (frontiersin.org)
EBPs1
  • The dimerization is necessary to enable C/EBPs to bind specifically to DNA through a palindromic sequence in the major groove of the DNA. (wikipedia.org)
Gene11
  • Nuclear factor 1 X-type is a protein that in humans is encoded by the NFIX gene. (wikipedia.org)
  • We have previously identified NFAT (nuclear factor of activated T-cells) as a key regulator of insulin gene transcription in pancreatic β-cells that is activated by the calcium/calmodulin-dependent protein phosphatase 2B (calcineurin) in response to increased [Ca 2+ ] i ( 10 ). (diabetesjournals.org)
  • NFAT binds to three distinct NFAT elements within the rat I insulin promoter and activates insulin gene transcription. (diabetesjournals.org)
  • The second pathway also involves glucose metabolism but appears to be driven by glucose-derived factors that target insulin gene transcription independently of [Ca 2+ ] i . (diabetesjournals.org)
  • Overlap of 2 suggestive SNPs with likely TF binding sites suggests possible roles in disruption of gene regulation. (cdc.gov)
  • p30C/EBP alpha binds to the C/EBP sites within and activates reporter gene expression driven by the 422(aP2) and C/EBP alpha gene promoters. (duke.edu)
  • Figure 1: Summary of estrogen receptor and RNA PolII binding sites and correlation with nucleotide and gene number. (nature.com)
  • Figure 3: Estrogen receptor and RNA PolII binding relative to specific gene targets. (nature.com)
  • In the version of this article initially published online, the estrogen receptor and RNA PolII binding data were aligned incorrectly in the top panel of Figure 3, which represents estrogen receptor binding at the ESR1 gene region. (nature.com)
  • Growth factor independence-1 (Gfi-1) plays a role in mediating specific granule deficiency (SGD) in a patient lacking a gene-inactivating mutation in the C/EBPepsilon gene. (lu.se)
  • Neutrophil-specific granule deficiency: homozygous recessive inheritance of a frameshift mutation in the gene encoding transcription factor CCAAT/enhancer binding protein--epsilon. (lu.se)
Regulatory3
  • Virgin mammary glands have high levels of full-length CDP that binds to negative regulatory elements (NRE) to repress MMTV transcription. (utexas.edu)
  • Our data demonstrate negative selection against binding sites alterations and suggest that such selection pressure protects cancer cells from rewiring of regulatory circuits. (biomedcentral.com)
  • The DNMT3A mutation can cause significant changes in epigenetic modifications and is one of the essential regulatory factors for the occurrence and development of leukemia ( 4 ). (frontiersin.org)
Induced plur1
  • Given that OKSM (Yamanaka) factors convert somatic cells into induced pluripotent stem (iPS) cells, alterations in transcriptional state could affect destiny of the cells. (intechopen.com)
Activator1
  • The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. (novusbio.com)
Granulocyte-macrop1
  • The results demonstrated that two cyclic AMP responsive elements (CREs) in the proximal promoter region of C/EBPβ were involved in the positive regulation of C/EBPβ transcription during granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced differentiation of bone marrow cells. (harvard.edu)
Cellular4
  • We investigated whether inhibition of hypoxia-inducible factor prolyl 4-hydroxylase-2 (HIF-P4H-2), a key cellular oxygen sensor whose inhibition stabilizes HIF, would protect from NAFLD by subjecting HIF-P4H-2-deficient ( Hif-p4h-2 gt/gt ) mice to a high-fat, high-fructose (HFHF) or high-fat, methionine-choline-deficient (HF-MCD) diet. (springer.com)
  • The cellular factor, CCAAT displacement protein (CDP), is a transcriptional repressor of MMTV. (utexas.edu)
  • Complement C11 binding protein (C1QBP, also named p32 and HABP1) is a multifunctional protein which plays a pivotal role in diverse cellular processes such autophagy and cell apoptosis [ 7 , 8 ]. (jcancer.org)
  • Description Inflammation, a complex process, involving numerous mediators of cellular and plasma origins, is considered to be a critical factor in many human diseases and conditions, including obesity, cardiovascular diseases, diabetes, aging, and cancers. (rutgers.edu)
Pathways4
  • Various cytokine/growth factor signaling pathways are abnormal in aged skin, particularly in the interleukin-1 family. (nih.gov)
  • This process activates protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK), inositol-requiring kinase 1 and activating transcription factor (ATF) 6, and initiates unfolded protein response (UPR) signaling pathways ( 13 , 14 ). (spandidos-publications.com)
  • Previously, we have shown that dysregulation of the WNT-signaling pathway and interference with the transcription factor IRF8 are major deregulated pathways in chronic myelogenous leukemia (CML). (mdc-berlin.de)
  • Furthermore, molecular docking was employed to predict the binding capacity of the primary bioactive components of LZP to the critical molecular protein targets in the IL-17 and TNF signaling pathways. (bvsalud.org)
Forkhead3
  • Forkhead box protein M1 (FOXM1) is a key transcription factor (TF) that regulates a common set of genes related to the cell cycle in various cell types. (mdpi.com)
  • Myostatin binding to type IIB activin receptor (ActRIIB) on muscle surface induces the recruitment and activation of activin receptor-like kinase 5 (ALK5), and eventually leads to forkhead box O3 (FoxO3a)-dependent transcription to promote muscle protein breakdown via the ubiquitin-proteasome system ( 23 ). (spandidos-publications.com)
  • It has been reported that Forkhead box transcription factor class O3a (Foxo3a) is expressed in rheumatoid arthritis, a chronic inflammatory condition accompanied by bone resorption, and plays a role in its pathology. (go.jp)
Eukaryotic3
  • Western blot analysis was used to measure eukaryotic initiation factor 2α (eIF2α) phosphorylation levels. (spandidos-publications.com)
  • As one of three primary UPR effectors, PERK directly phosphorylates eukaryotic initiation factor 2α (eIF2α), which consequently inhibits initiation of general translation and reduces ER burden ( 15 ). (spandidos-publications.com)
  • Neuronal nitric oxide synthase (nNOS) and vitamin D receptor (VDR) expression were measured, as well as ER stress markers, including glucose-regulated protein78 (GRP78), protein kinase-like endoplasmic reticulum kinase (PERK) phosphorylation, eukaryotic initiation factor 2 alpha(eIF-2 alpha) phosphorylation, and CCAAT enhancer-binding protein homologous protein (CHOP). (cheric.org)
Genes3
  • For example, steroid and thyroid hormones directly bind to nuclear receptors, which induce expression of specific genes. (intechopen.com)
  • Furthermore, the loss of DNA-binding activity correlates with increased expression of MMTV and other mammary-specific genes, including β-casein, whey acidic protein and α-lactalbumin, indicating that CDP150 is a developmentally controlled, naturally occurring dominant-negative protein. (utexas.edu)
  • We mapped all estrogen receptor and RNA polymerase II binding sites on a genome-wide scale, identifying the authentic cis binding sites and target genes, in breast cancer cells. (nature.com)
Differentiation2
  • C/EBPβ plays a role in neuronal differentiation, in learning, in memory processes, in glial and neuronal cell functions, and in neurotrophic factor expression. (wikipedia.org)
  • The transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) is expressed in Pax7 + satellite cells of healthy muscle and is downregulated during myoblast differentiation. (biomedcentral.com)
Phosphorylation1
  • Consistent with this observation, cAMP elevation in HUVECs produced a transient yet robust activation of ERK, and subsequent phosphorylation of transcription factor C/EBPβ, both of which were resistant to PKA inhibition. (gla.ac.uk)
CREB1
  • CREB was upregulated, phosphorylated and bound to the CREs in response to GM-CSF stimulation. (harvard.edu)
Affinity2
  • The point-mutated Cyp51A has lower binding affinity to azole drugs, causing azole resistance in these strains ( 5 ). (cdc.gov)
  • Assessment of potential binding affinity changes induced by substitutions allows studying selection of sequence variants in binding sites in a way resembling usage of non-synonymous and synonymous substitutions in codons. (biomedcentral.com)
Transcripts1
  • During late pregnancy, CDP DNA-binding activity declines concomitant with an increase in viral transcripts. (utexas.edu)
Cytokine1
  • After the co-culture, the increase in pro-inflammatory cytokine expression in the mutant cells was significantly lower than that in the control group, while that in immunosuppressive factors was not significantly different. (frontiersin.org)
Glucose1
  • Glucose and GLP-1 also synergistically activated NFAT (nuclear factor of activated T-cells)-mediated transcription from a minimal promoter construct containing tandem NFAT consensus sequences. (diabetesjournals.org)
Regions1
  • Such stability of binding motifs is even more exhibited in DNase accessible regions. (biomedcentral.com)
Ligand1
  • Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is known to cause apoptosis in several types of malignant tumor cells through its interaction with the death domain-containing receptor, death receptor 5 (DR5). (nih.gov)
Inflammatory3
  • Barnes, P.J. & Karin, M. Nuclear factor-κB: a pivotal transcription factor in chronic inflammatory diseases. (nature.com)
  • Increasing evidence indicates that pro-inflammatory mediators, protein degradation-associated factors, and some other circulating mediators drive this process ( 18 ). (spandidos-publications.com)
  • In co-cultivated supernatants, the concentration of inflammatory factors in the experimental group was significantly lower than that in the control group, while that of immunosuppressive factors was significantly higher. (frontiersin.org)
Molecular1
  • Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)
Sites1
  • Figure 4: Identification of enriched motifs within the estrogen receptor binding sites and validation of transcription factor binding. (nature.com)
Motifs1
  • Here we present analysis of transcription factor binding motifs co-localized with non-coding variants. (biomedcentral.com)
Growth3
  • A large number of studies have demonstrated that the synergistic collaboration of a number of microRNAs (miRNAs), their growth factors and their downstream agents is required for the initiation and completion of pathogenesis in the liver. (wjgnet.com)
  • In addition, RPE cells proliferate and secrete various proangiogenic factors, including vascular endothelial growth factor (VEGF), which serves an important role in AMD-associated CNV ( 4 ). (spandidos-publications.com)
  • These results suggested that environmental factors are important in selectively favoring yeast or hyphal form, most important being the growth medium, incubation temperature and external pH value. (scirp.org)
Initiation1
  • First, the general TFs (GTFs), including preinitiation complex components TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and THIIH, are the primary protein factors that are required for the initiation of transcription from the TATA box (or TATA element), then elongation is executed by RNA polymerase II (RNA pol II) [ 1 ]. (intechopen.com)
Role3
  • A recent study showed that HapE forms a CCAAT binding complex, which plays a role in the negative regulation of cyp51A expression. (cdc.gov)
  • In contrast to the definitive role of the transcription factor, CCAAT/Enhancer binding protein α (C/EBPα), in steady-state granulopoiesis, previous findings have suggested that granulopoiesis during emergency situations, such as infection, is dependent on C/EBPβ. (harvard.edu)
  • Although a variety of studies have shown that Complement C11 binding protein (C1QBP) may play a tumor-promoting or tumor-suppressive role in cancer, the functions and mechanisms of C1QBP in HCC progression are under-investigating. (jcancer.org)
Cassette1
Expression1
  • The expression of cdr1B is dependent on AtrR, a recently characterized transcriptional factor ( 12 ), but the reason for the constitutive expression remains unclear. (cdc.gov)

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