Caveolin 2 is a binding partner of CAVEOLIN 1. It undergoes tyrosine phosphorylation by C-SRC PROTEIN PP60 and plays a regulatory role in CAVEOLAE formation.
Endocytic/exocytic CELL MEMBRANE STRUCTURES rich in glycosphingolipids, cholesterol, and lipid-anchored membrane proteins that function in ENDOCYTOSIS (potocytosis), transcytosis, and SIGNAL TRANSDUCTION. Caveolae assume various shapes from open pits to closed vesicles. Caveolar coats are composed of CAVEOLINS.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Detergent-insoluble CELL MEMBRANE components. They are enriched in SPHINGOLIPIDS and CHOLESTEROL and clustered with glycosyl-phosphatidylinositol (GPI)-anchored proteins.
A complex of polyene antibiotics obtained from Streptomyces filipinensis. Filipin III alters membrane function by interfering with membrane sterols, inhibits mitochondrial respiration, and is proposed as an antifungal agent. Filipins I, II, and IV are less important.
Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.
A partitioning within cells due to the selectively permeable membranes which enclose each of the separate parts, e.g., mitochondria, lysosomes, etc.
The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.
Cyclic GLUCANS consisting of seven (7) glucopyranose units linked by 1,4-glycosidic bonds.
The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. Each molecule of clathrin consists of three light chains (CLATHRIN LIGHT CHAINS) and three heavy chains (CLATHRIN HEAVY CHAINS) that form a structure called a triskelion. Clathrin also interacts with cytoskeletal proteins.
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Nonionic surfactant mixtures varying in the number of repeating ethoxy (oxy-1,2-ethanediyl) groups. They are used as detergents, emulsifiers, wetting agents, defoaming agents, etc. Octoxynol-9, the compound with 9 repeating ethoxy groups, is a spermatocide.
A homologous group of cyclic GLUCANS consisting of alpha-1,4 bound glucose units obtained by the action of cyclodextrin glucanotransferase on starch or similar substrates. The enzyme is produced by certain species of Bacillus. Cyclodextrins form inclusion complexes with a wide variety of substances.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Established cell cultures that have the potential to propagate indefinitely.
A CALCIUM-dependent, constitutively-expressed form of nitric oxide synthase found primarily in ENDOTHELIAL CELLS.
Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays.
Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.
Neoplasms composed of fatty tissue or connective tissue made up of fat cells in a meshwork of areolar tissue. The concept does not refer to neoplasms located in adipose tissue.
A subtype of dynamin found ubiquitously expressed in a variety of tissues.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Cells in the body that store FATS, usually in the form of TRIGLYCERIDES. WHITE ADIPOCYTES are the predominant type and found mostly in the abdominal cavity and subcutaneous tissue. BROWN ADIPOCYTES are thermogenic cells that can be found in newborns of some species and hibernating mammals.
Compounds containing carbohydrate or glycosyl groups linked to phosphatidylinositols. They anchor GPI-LINKED PROTEINS or polysaccharides to cell membranes.
Membrane-limited structures derived from the plasma membrane or various intracellular membranes which function in storage, transport or metabolism.
Short tracts of DNA sequence that are used as landmarks in GENOME mapping. In most instances, 200 to 500 base pairs of sequence define a Sequence Tagged Site (STS) that is operationally unique in the human genome (i.e., can be specifically detected by the polymerase chain reaction in the presence of all other genomic sequences). The overwhelming advantage of STSs over mapping landmarks defined in other ways is that the means of testing for the presence of a particular STS can be completely described as information in a database.
A family of high molecular weight GTP phosphohydrolases that play a direct role in vesicle transport. They associate with microtubule bundles (MICROTUBULES) and are believed to produce mechanical force via a process linked to GTP hydrolysis. This enzyme was formerly listed as EC 3.6.1.50.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
An NADPH-dependent enzyme that catalyzes the conversion of L-ARGININE and OXYGEN to produce CITRULLINE and NITRIC OXIDE.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The extension of endometrial tissue (ENDOMETRIUM) into the MYOMETRIUM. It usually occurs in women in their reproductive years and may result in a diffusely enlarged uterus with ectopic and benign endometrial glands and stroma.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Cytoplasmic vesicles formed when COATED VESICLES shed their CLATHRIN coat. Endosomes internalize macromolecules bound by receptors on the cell surface.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Centrifugation using a rotating chamber of large capacity in which to separate cell organelles by density-gradient centrifugation. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The protein constituents of muscle, the major ones being ACTINS and MYOSINS. More than a dozen accessory proteins exist including TROPONIN; TROPOMYOSIN; and DYSTROPHIN.
Amino acids containing an aromatic side chain.
A glucose transport protein found in mature MUSCLE CELLS and ADIPOCYTES. It promotes transport of glucose from the BLOOD into target TISSUES. The inactive form of the protein is localized in CYTOPLASMIC VESICLES. In response to INSULIN, it is translocated to the PLASMA MEMBRANE where it facilitates glucose uptake.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Src-family kinases that associate with T-CELL ANTIGEN RECEPTOR and phosphorylate a wide variety of intracellular signaling molecules.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.
Unsaturated derivatives of the steroid androstane containing at least one double bond at any site in any of the rings.
Transport proteins that carry specific substances in the blood or across cell membranes.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
A PROTEIN-TYROSINE KINASE family that was originally identified by homology to the Rous sarcoma virus ONCOGENE PROTEIN PP60(V-SRC). They interact with a variety of cell-surface receptors and participate in intracellular signal transduction pathways. Oncogenic forms of src-family kinases can occur through altered regulation or expression of the endogenous protein and by virally encoded src (v-src) genes.
A genus of owlet moths of the family Noctuidae. These insects are used in molecular biology studies during all stages of their life cycle.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
An enzyme that catalyzes the oxidation of cholesterol in the presence of molecular oxygen to 4-cholesten-3-one and hydrogen peroxide. The enzyme is not specific for cholesterol, but will also oxidize other 3-hydroxysteroids. EC 1.1.3.6.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A subtype of thioredoxin reductase found primarily in the CYTOSOL.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
A heterogeneous group of inherited MYOPATHIES, characterized by wasting and weakness of the SKELETAL MUSCLE. They are categorized by the sites of MUSCLE WEAKNESS; AGE OF ONSET; and INHERITANCE PATTERNS.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Proteins prepared by recombinant DNA technology.
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.
A 700-kDa cytosolic protein complex consisting of seven equimolar subunits (alpha, beta, beta', gamma, delta, epsilon and zeta). COATOMER PROTEIN and ADP-RIBOSYLATION FACTOR 1 are principle components of COAT PROTEIN COMPLEX I and are involved in vesicle transport between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.
GPI-linked membrane proteins broadly distributed among hematopoietic and non-hematopoietic cells. CD55 prevents the assembly of C3 CONVERTASE or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex.
A low affinity receptor that binds NERVE GROWTH FACTOR; BRAIN-DERIVED NEUROTROPHIC FACTOR; NEUROTROPHIN 3; and neurotrophin 4.
A large group of membrane transport proteins that shuttle MONOSACCHARIDES across CELL MEMBRANES.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles is covered with a lattice-like network of the protein CLATHRIN. Shortly after formation, however, the clathrin coat is removed and the vesicles are referred to as ENDOSOMES.
Enzymes that hydrolyze GTP to GDP. EC 3.6.1.-.
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
A specific monosialoganglioside that accumulates abnormally within the nervous system due to a deficiency of GM1-b-galactosidase, resulting in GM1 gangliosidosis.
Lipids containing at least one monosaccharide residue and either a sphingoid or a ceramide (CERAMIDES). They are subdivided into NEUTRAL GLYCOSPHINGOLIPIDS comprising monoglycosyl- and oligoglycosylsphingoids and monoglycosyl- and oligoglycosylceramides; and ACIDIC GLYCOSPHINGOLIPIDS which comprises sialosylglycosylsphingolipids (GANGLIOSIDES); SULFOGLYCOSPHINGOLIPIDS (formerly known as sulfatides), glycuronoglycosphingolipids, and phospho- and phosphonoglycosphingolipids. (From IUPAC's webpage)
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
GTP-BINDING PROTEINS that contain three non-identical subunits. They are found associated with members of the seven transmembrane domain superfamily of G-PROTEIN-COUPLED RECEPTORS. Upon activation the GTP-BINDING PROTEIN ALPHA SUBUNIT of the complex dissociates leaving a dimer of a GTP-BINDING PROTEIN BETA SUBUNIT bound to a GTP-BINDING PROTEIN GAMMA SUBUNIT.
A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.
An essential amino acid that is required for the production of HISTAMINE.
A family of heterotrimeric GTP-binding protein alpha subunits that were originally identified by their ability to inhibit ADENYLYL CYCLASES. Members of this family can couple to beta and gamma G-protein subunits that activate POTASSIUM CHANNELS. The Gi-Go part of the name is also spelled Gi/Go.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
The entering of cells by viruses following VIRUS ATTACHMENT. This is achieved by ENDOCYTOSIS, by direct MEMBRANE FUSION of the viral membrane with the CELL MEMBRANE, or by translocation of the whole virus across the cell membrane.

NO synthase II in mouse skeletal muscle is associated with caveolin 3. (1/243)

The inducible-type NO synthase (NOS II; iNOS) is constitutively expressed in slow-twitch skeletal muscle fibres of guinea-pigs [Gath, Closs, Godtel-Armbrust, Schmitt, Nakane, Wessler and Forstermann (1996) FASEB J. 10, 1614-1620]. Here we studied the expression of NOS II in skeletal muscle of wild-type and NOS II-deficient mice and investigated the molecular basis for the membrane association of this NOS in muscle. A basal expression of NOS II mRNA and protein was detected in skeletal muscle from untreated wild-type mice; expression increased when mice were treated with bacterial lipopolysaccharide (LPS). No NOS II was found in any tissue of untreated or LPS-treated NOS II-deficient mice. Immunoprecipitation experiments were performed with homogenates of gastrocnemius muscle from untreated or LPS-treated wild-type mice. A NOS II-specific antibody precipitated caveolin 3 in all homogenates investigated, the effect being most pronounced in skeletal muscle from LPS-treated animals. Conversely, an antibody against caveolin 3 co-precipitated NOS II in muscle homogenates. Similarly, a weak co-precipitation of NOS II and caveolin 3 was seen in homogenates of untreated murine C2C12 myotubes; co-precipitation was markedly enhanced in cells stimulated with LPS/interferon gamma. The association of NOS II with caveolin 3 might have implications for the regulation of contraction of, and/or glucose uptake by, slow-twitch muscle fibres.  (+info)

Neuregulin signaling in the heart. Dynamic targeting of erbB4 to caveolar microdomains in cardiac myocytes. (2/243)

Two of the neuregulins (NRG1 and NRG2) and their receptors (erbB2 and erbB4) are essential for normal cardiac development and can mediate hypertrophic growth and enhance survival of embryonic, postnatal, and adult rat ventricular myocytes. The expression of erbB4, the predominant NRG receptor in postnatal rat ventricular muscle, declines after midembryogenesis, and its expression is limited to cardiac myocytes. A full-length erbB4 rat cDNA isolated from neonatal ventricular muscle was found to be highly homologous to human erbB4 and contained a caveolin binding motif within the cytoplasmic kinase domain. Using the complementary techniques of detergent-free density-gradient ultracentrifugation of myocyte lysates and coimmunoprecipitation of erbB4 and caveolin-3, the caveolin isoform expressed in cardiac myocytes, erbB4 could be localized (using both approaches) to caveolar microdomains. Moreover, addition of a soluble NRG1, recombinant human glial growth factor 2, resulted in rapid (2-minute) translocation of erbB4 out of caveolar microdomain in cardiac myocytes. Thus, erbB4 is dynamically targeted to caveolar microdomains within cardiac myocytes. Its rapid translocation after NRG1 binding may contribute to receptor desensitization in the continuous presence of ligand.  (+info)

Molecular characterization of caveolin association with the Golgi complex: identification of a cis-Golgi targeting domain in the caveolin molecule. (3/243)

Caveolins are integral membrane proteins which are a major component of caveolae. In addition, caveolins have been proposed to cycle between intracellular compartments and the cell surface but the exact trafficking route and targeting information in the caveolin molecule have not been defined. We show that antibodies against the caveolin scaffolding domain or against the COOH terminus of caveolin-1 show a striking specificity for the Golgi pool of caveolin and do not recognize surface caveolin by immunofluorescence. To analyze the Golgi targeting of caveolin in more detail, caveolin mutants were expressed in fibroblasts. Specific mutants lacking the NH2 terminus were targeted to the cis Golgi but were not detectable in surface caveolae. Moreover, a 32-amino acid segment of the putative COOH-terminal cytoplasmic domain of caveolin-3 was targeted specifically and exclusively to the Golgi complex and could target a soluble heterologous protein, green fluorescent protein, to this compartment. Palmitoylation-deficient COOH-terminal mutants showed negligible association with the Golgi complex. This study defines unique Golgi targeting information in the caveolin molecule and identifies the cis Golgi complex as an intermediate compartment on the caveolin cycling pathway.  (+info)

Localization of the human caveolin-3 gene to the D3S18/D3S4163/D3S4539 locus (3p25), in close proximity to the human oxytocin receptor gene. Identification of the caveolin-3 gene as a candidate for deletion in 3p-syndrome. (4/243)

Caveolin-3, a muscle-specific caveolin-related protein, is the principal structural protein of caveolae membrane domains in striated muscle cell types (cardiac and skeletal). Recently, we identified an autosomal dominant form of limb girdle muscular dystrophy in humans that is due to mutations within exon 2 of the caveolin-3 gene (3p25). However, the detailed location of the human caveolin-3 gene and its position with regard to neighboring genes remains unknown. Here, we have isolated three independent BAC clones containing the human caveolin-3 gene. Using a PCR-based approach, we determined that these clones contain both exons 1 and 2 of the human caveolin-3 gene. In addition, we performed microsatellite marker analysis of these BAC clones, using a panel of 13 markers that are known to map within the 3p25 region. Our results indicate that these BAC clones contain the following three markers: D3S18, SHGC-1079 (also known as D3S4163) and D3S4539. Interestingly, D3S18 is a marker for two known human diseases, von Hippel-Lindau disease and 3p-syndrome. As D3S4163 and D3S4539 are known to map in the vicinity of the 3' end of the human oxytocin receptor gene, we determined if these caveolin-3 positive BACs also contain the oxytocin receptor gene. We show that (i) these BACs contain all four exons of the oxytocin receptor gene and (ii) that the genes encoding caveolin-3 and the oxytocin receptor are located approximately 7-10 kb apart and in the opposite orientation. As 3p-syndrome is characterized by cardiac septal defects and caveolin-3 is expressed primarily in the heart and skeletal muscle, caveolin-3 is a candidate gene that may be deleted in 3p-syndrome.  (+info)

Caveolin-3 upregulation activates beta-secretase-mediated cleavage of the amyloid precursor protein in Alzheimer's disease. (5/243)

Here, we investigate the involvement of caveolins in the pathophysiology of Alzheimer's disease (AD). We show dramatic upregulation of caveolin-3 immunoreactivity in astroglial cells surrounding senile plaques in brain tissue sections from authentic AD patients and an established transgenic mouse model of AD. In addition, we find that caveolin-3 physically interacts and biochemically colocalizes with amyloid precursor protein (APP) both in vivo and in vitro. Interestingly, recombinant overexpression of caveolin-3 in cultured cells stimulated beta-secretase-mediated processing of APP. Immunoreactivities of APP and presenilins were concomitantly increased in caveolin-3-positive astrocytes. Because the presenilins also form a physical complex with caveolin-3, caveolin-3 may provide a common platform for APP and the presenilins to associate in astrocytes. In AD, augmented expression of caveolin-3 and presenilins in reactive astrocytes may alter APP processing, leading to the overproduction of its toxic amyloid metabolites.  (+info)

Phenotypic behavior of caveolin-3 mutations that cause autosomal dominant limb girdle muscular dystrophy (LGMD-1C). Retention of LGMD-1C caveolin-3 mutants within the golgi complex. (6/243)

Caveolin-3, a muscle-specific caveolin-related protein, is the principal structural protein of caveolae membrane domains in striated muscle cell types (cardiac and skeletal). Autosomal dominant limb girdle muscular dystrophy (LGMD-1C) in humans is due to mutations within the caveolin-3 gene: (i) a 9-base pair microdeletion that removes three amino acids within the caveolin scaffolding domain (DeltaTFT) or (ii) a missense mutation within the membrane spanning domain (P --> L). The molecular mechanisms by which these two mutations cause muscular dystrophy remain unknown. Here, we investigate the phenotypic behavior of these caveolin-3 mutations using heterologous expression. Wild type caveolin-3 or caveolin-3 mutants were transiently expressed in NIH 3T3 cells. LGMD-1C mutants of caveolin-3 (DeltaTFT or P --> L) were primarily retained at the level of a perinuclear compartment that we identified as the Golgi complex in double-labeling experiments, while wild type caveolin-3 was efficiently targeted to the plasma membrane. In accordance with these observations, caveolin-3 mutants formed oligomers of a much larger size than wild type caveolin-3 and were excluded from caveolae-enriched membrane fractions as seen by sucrose density gradient centrifugation. In addition, these caveolin-3 mutants were expressed at significantly lower levels and had a dramatically shortened half-life of approximately 45-60 min. However, caveolin-3 mutants were palmitoylated to the same extent as wild type caveolin-3, indicating that targeting to the plasma membrane is not required for palmitoylation of caveolin-3. In conclusion, we show that LGMD-1C mutations lead to formation of unstable high molecular mass aggregates of caveolin-3 that are retained within the Golgi complex and are not targeted to the plasma membrane. Consistent with its autosomal dominant form of genetic transmission, we demonstrate that LGMD-1C mutants of caveolin-3 behave in a dominant-negative fashion, causing the retention of wild type caveolin-3 at the level of the Golgi. These data provide a molecular explanation for why caveolin-3 levels are down-regulated in patients with this form of limb girdle muscular dystrophy (LGMD-1C).  (+info)

The limb-girdle muscular dystrophies-multiple genes, multiple mechanisms. (7/243)

In the field of muscular dystrophy, advances in understanding the molecular basis of the various disorders in this group have been rapidly translated into readily applicable diagnostic tests, allowing the provision of more accurate prognostic and genetic counselling. The limb-girdle muscular dystrophies (LGMD) have recently undergone a major reclassification according to their genetic basis. Currently 13 different types can be recognized. Amongst this group, increasing diversity of the mechanisms involved in producing a muscular dystrophy phenotype is emerging. Recent insights into the involvement of the dystrophin glycoprotein complex in muscular dystrophy suggests that its members may play distinct or even multiple roles in the maintenance of muscle fibre integrity. In other forms of LGMD, proteins have been implicated which may be important in intracellular signalling, vesicle trafficking or the control of transcription. As these various mechanisms are more fully elucidated, further insights will be gained into the pathophysiology of muscular dystrophy. At a practical level, despite the marked heterogeneity of this group real progress can at last be made in determining a precise diagnosis.  (+info)

Codistribution of NOS and caveolin throughout peripheral vasculature and skeletal muscle of hamsters. (8/243)

In isolated cell systems, nitric oxide synthase (NOS) activity is regulated by caveolin (CAV), a resident caveolae coat protein. Because little is known of this interaction in vivo, we tested whether NOS and caveolin are distributed together in the intact organism. Using immunohistochemistry, we investigated the localization of constitutive neuronal (nNOS) and endothelial (eNOS) enzyme isoforms along with caveolin-1 (CAV-1) and caveolin-3 (CAV-3) throughout the systemic vasculature and peripheral tissues of the hamster. The carotid artery, abdominal aorta, vena cava, femoral artery and vein, feed artery and collecting vein of the cheek pouch retractor muscle, capillaries and muscle fibers of retractor and cremaster muscles, and arterioles and venules of the cheek pouch were studied. In endothelial cells, eNOS and CAV-1 were present throughout the vasculature, whereas nNOS and CAV-3 were absent except in capillaries, which reacted for nNOS. In smooth muscle cells, nNOS and CAV-1 were also expressed systemically, whereas eNOS was absent; CAV-3 was present in the arterial but not the venous vasculature. Both nNOS and CAV-3 were located at the sarcolemma of skeletal muscle fibers, which were devoid of eNOS and CAV-1. These immunolabeling patterns suggest functional interactions between eNOS and CAV-1 throughout the endothelium, regional differences in the modulation of nNOS by caveolin isoforms in vascular smooth muscle, and modulation of nNOS by CAV-3 in skeletal muscle.  (+info)

This issue contains an important contribution from the team of Douglas Lauffenburger, Chair of the Editorial Board, at MIT, USA, in which they demonstrate the ability to characterise quantitative data on phenotypic behaviour of individual endothelial cells in response to angiogenesis signalling and relate it to overall population behaviour. They show that the behaviour of microvascular endothelial cells in a single population is heterogeneous and cannot be assumed to be the same for all cells in a given condition.. Endothelial cell phenotypic behaviors cluster into dynamic state transition programs modulated by angiogenic and angiostatic ...
Title: Coordinated Expression of Pax-5 and FAK1 in Metastasis. VOLUME: 11 ISSUE: 7. Author(s):Nicolas Crapoulet, Pierre OBrien, Rodney J. Ouellette and Gilles A. Robichaud. Affiliation:Universite de Moncton, Moncton, NB, Canada, E1A 3E9.. Keywords:Cancer, cell signaling, FAK1, focal adhesion, metastasis, Pax-5, B lymphopoiesis, cell differentiation, homeostasis, leukemia. Abstract: The Pax-5 gene encodes a B-cell-specific activator protein (BSAP) that plays a key role in B lymphocyte differentiation and embryogenesis. The deregulation of this transcription factor is also linked to B cell malignancies and recently to other cancers. More specifically, the downstream effects of Pax-5 promote cell-cell interactions and mediate the activation of adhesion genes which result in an epithelial phenotypic behavior of human carcinoma cells. To gain a better understanding of Pax-5-mediated gene regulation, we studied available gene expression data in depth and identified several Pax-5 downstream targets. ...
The proteoglycan-dystrophin complex in genetic cardiomyopathies--lessons from three siblings with limb-girdle muscular dystrophy-2I (LGMD-2I).:
www.MOLUNA.de Caveolins in Cancer Pathogenesis, Prevention and Therapy [4196990] - Caveolins play an important role in the pathogenesis of multiple cancers. This volume focuses mainly on the importance of Caveolin-1 in breast, prostate, lung, skin, colon, pancreatic and brain cancers. It also studies the role of Caveolin-3 in breast cancer.Caveolins are important structural proteins of Caveolae, small invaginations of the
To increase tolerance to abiotic stresses in breeding programmes, typically families and collections of genotypes are evaluated in series of trials (environments) representing different levels of stress. The statistical analysis of the data from such trials concentrates on modelling the phenotypic behaviour of the genotypes across the set of environments. This phenotypic behaviour can be modelled in the form of genotype-specific linear and non-linear response curves in relation to environmental characterizations. Non-parallelism of the response curves indicates genotype × environment interaction. Identification of the genetic basis of the parameters determining the response curves will help in the development of breeding programmes for improving abiotic stress tolerance and understanding genotype × environment interaction. In this paper we present two strategies for locating quantitative trait loci for response-curve parameters and estimation of their allele effects. The procedures are ...
Caveolin-3, the muscle-specific isoform of caveolins, plays important roles in signal transduction. Dominant-negative mutations of the caveolin-3 gene cause autosomal dominant limb-girdle muscular dystrophy 1C (LGMD1C) with loss of caveolin-3. However, identification of the precise molecular mechanism leading to muscular atrophy in caveolin-3-deficient muscle has remained elusive. Myostatin, a member of the muscle-specific TGF-β superfamily, negatively regulates skeletal muscle volume. Here we report that caveolin-3 inhibited myostatin signaling by suppressing activation of its type I receptor; this was followed by hypophosphorylation of an intracellular effector, Mad homolog 2 (Smad2), and decreased downstream transcriptional activity. Loss of caveolin-3 in P104L mutant caveolin-3 transgenic mice caused muscular atrophy with increase in phosphorylated Smad2 (p-Smad2) as well as p21 (also known as Cdkn1a), a myostatin target gene. Introduction of the myostatin prodomain, an inhibitor of ...
At the European Molecular Biology Laboratory (EMBL) in Heidelberg, Professor Parton was mentored by Gareth Griffiths, where he honed his skills in electron microscopy while collaborating with other groups throughout the institute including Jean Gruenberg and Marino Zerial. He has maintained these collaborations to this day, despite the groups now being spread around the globe.. Collaboration has been central to his career. In a collaborative project with the laboratory of Kai Simons, he observed that the newly-discovered protein, VIP21 (later renamed caveolin-1), was an abundant marker protein of caveolae. Also at the EMBL Parton and Michael Way discovered a second member of the caveolin family, M-caveolin, now termed caveolin-3.. At The University of Queensland he collaborated with Professor John Hancock. Together they showed that caveolin mutants can act as dominant negative inhibitory mutants and that one of the mutants was a highly potent inhibitor of Ras signalling. Inhibition was specific ...
opamine D3 receptor, Caveolin1, Caveolin 1, 3 dopamine receptor, D, Dopamine receptor D3, DRD, ETM1, FET1, Cav-1, ETM1, CAV 1, CAV1, CAV1_HUMAN.
This download caveolins was been, and received on the treatment by Sir Henry Irving in 1875. Harold was in 1876, The Cup in 1881( at the Lyceum), The Promise of May( at the Globe) in 1882, Becket in 1884, with The Foresters in 1892. The download is one from which I give, not right of secure single-player of the purchase and of of rendering for the analysis.
Biochemical network reconstructions have become popular tools in systems biology. Metabolicnetwork reconstructions are biochemically, genetically, and genomically (BiGG) structured databases of biochemical reactions and metabolites. They contain information such as exact reaction stoichiometry, reaction reversibility, and the relationships between genes, proteins, and reactions. Network reconstructions have been used extensively to study the phenotypic behavior of wild-type and mutant stains under a variety of conditions, linking genotypes with phenotypes. Such phenotypic simulations have allowed for the prediction of growth after genetic manipulations, prediction of growth phenotypes after adaptive evolution, and prediction of essential genes. Additionally, because network reconstructions are organism specific, they can be used to understand differences between organisms of species in a functional context.There are different types of reconstructions representing various types of biological networks
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This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites. [provided by RefSeq, Jul 2008 ...
Expression of caveolins in RMS tumours. Double immunostain showing that in skeletal muscle Cav-1 and Cav-3 mark satellite cells and the plasmalemma of myofibres
For phenotypic behavior to be understood in the context of cell lineage and local environment, properties of individual cells must be measured relative to population-wide traits. However, the inability to accurately identify, track and measure thousands of single cells via high-throughput microscopy has impeded dynamic studies of cell populations. We demonstrate unique labeling of cells, driven by the heterogeneous random uptake of fluorescent nanoparticles of different emission colors. By sequentially exposing a cell population to different particles, we generated a large number of unique digital codes, which corresponded to the cell-specific number of nanoparticle-loaded vesicles and were visible within a given fluorescence channel. When three colors are used, the assay can self-generate over 17,000 individual codes identifiable using a typical fluorescence microscope. The color-codes provided immediate visualization of cell identity and allowed us to track human cells with a success rate of ...
A pacing system delivers cardiac protection pacing to protect the heart from injuries associated with ischemic events. The pacing system detects an ischemic event and, in response, initiates one or more cardiac protection pacing sequences each including alternative pacing and non-pacing periods. In one embodiment, the pacing system initiates cardiac protection pacing sequences including at least one postconditioning sequence to protect the heart from a detected ischemic event and a plurality prophylactic preconditioning sequences to protect the heart from probable future ischemic events.
A pacing system delivers cardiac protection pacing to protect the heart from injuries associated with ischemic events. The pacing system detects an ischemic event and, in response, initiates one or more cardiac protection pacing sequences each including alternative pacing and non-pacing periods. In one embodiment, the pacing system initiates a cardiac protection pacing sequence in response to the detection of the onset of an ischemic event, such that a pacing concurrent conditioning therapy is applied during the detected ischemic event.
Dysferlin兔多克隆抗体(ab15108)可与狗, 人样本反应并经WB, IHC, ICC/IF实验严格验证,被3篇文献引用并得到3个独立的用户反馈。所有产品均提供质保服务,中国75%以上现货。
Caveolae are specialized flask-shaped lipid rafts enriched in cholesterol, sphingolipids, and structural marker proteins termed caveolins. Caveolins are highly conserved hairpin loop-shaped, oligomeric proteins of 22-24 kDa. Besides the plasma cell membrane, caveolins are also present in mitochondri …
The limb‐girdle muscular dystrophies (LGMDs) area group of genetically heterogeneous neuromuscular disorders caused by specific protein defects in muscle fibres and characterised by predominant weakness and wasting in proximal limb and axial muscles
TY - CHAP. T1 - Role of caveolae in the airway. AU - Pabelick, Christina M. AU - Singh, Brij B.. AU - Prakash, Y.s.. PY - 2013/11/1. Y1 - 2013/11/1. N2 - Caveolae are flask-shaped invaginations of the plasma membrane that are rich in lipids and serve as microdomains to facilitate interactions between proteins at the membrane as well as intracellular components, thus modulating signal transduction, protein and lipid transport, and other processes. Constituent caveolar proteins such as caveolins and cavins also have scaffolding domains that anchor and regulate protein function. There is now evidence that caveolae and their constituent proteins are present in airway smooth muscle in a variety of species. Caveolae in airway cells contain or interact with molecules such as receptors, ion channels, and regulatory proteins that are key to the roles of airway epithelium and smooth muscle in regulating airway structure and function. Furthermore, caveolar protein expression and regulation appear to be ...
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity).
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Caveolin-2兔单克隆抗体[EPR2220(3)](ab124883)可与人样本反应并经WB, Flow Cyt实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Plasmid CAV1-mEGFP from Dr. Ari Heleniuss lab contains the insert caveolin-1 and is published in Traffic. 2010 Mar . 11(3):361-82. This plasmid is available through Addgene.
In this study, we examined cardiomyocyte hypertrophy by several methods. PE and ET increased leucine incorporation and cell area and changed the immunostaining pattern of phalloidin from a dense staining without agonists to fiberlike staining, indicating that myocyte hypertrophy did occur at the protein and structural levels. Moreover, βMHC expression was markedly augmented by PE and ET, suggesting the transformation of myosin. These results indicate that PE and ET induced pathologic hypertrophy in cardiomyocytes. Infection with Ad.Cav-3 prevented all of these changes induced by PE and ET. Thus, caveolin-3 might act as an inhibitor of myocyte hypertrophy. The effects of overexpression of wild-type caveolin-3 were not nonspecific, because infection with Ad.LacZ did not affect myocyte hypertrophy. Overexpression of caveolin-3 in Ad.Cav-3-infected cells was associated with the prevention of both agonist-induced hypertrophy and sarcomeric disorganization seen in Ad.LacZ-infected and control cells ...
Caveolins act as scaffold proteins in multiprotein complexes and have been implicated in signaling by G protein-coupled receptors. Studies using knock-out mice suggest that beta(3)-adrenoceptor (beta(3)-AR) signaling is dependent on caveolin-1; however, it is not known whether caveolin-1 is associated with the beta(3)-AR or solely with downstream signaling proteins. We have addressed this question by examining the impact of membrane rafts and caveolin-1 on the differential signaling of mouse beta(3a)- and beta(3b)-AR isoforms that diverge at the distal C terminus. Only the beta(3b)-AR promotes pertussis toxin (PTX)-sensitive cAMP accumulation. When cells expressing the beta(3a)-AR were treated with filipin III to disrupt membrane rafts or transfected with caveolin-1 siRNA, the cyclic AMP response to the beta(3)-AR agonist CL316243 became PTX-sensitive, suggesting G alpha(i/o) coupling. The beta(3a)-AR C terminus, S (P-384) under bar PLNR (P-389) under bar DG (Y-392) under bar EGARP (P-398) under ...
Coronary Artery disease is one of the leading causes of death in china, followed by cancer. According to US CDC (Centre for Disease Control), cardiovascular disease is also the leading cause of death of men and women in the United States. Factors including hereditary and family history, nutrition and lifestyle, hypertension, high lipids and cholesterol etc, will increase the risk for cardiovascular diseases. A persons unique genetic makeup will also impact their responses to drugs eg. dosage, side effects etc. Cardiac Protection Genetic Test is conducted a CAP and CLIA accredited genetic laboratory in California US.. ...
Buy our Caveolin-2 peptide. Ab4929 is a blocking peptide and has been validated in BL. Abcam provides free protocols, tips and expert support for WB and a 12…
Caveolin-3 is a protein that in humans is encoded by the CAV3 gene. Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites. This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), HyperCKemia, distal myopathy or rippling muscle disease (RMD). Other mutations in Caveolin causes Long QT Syndrome or familial hypertrophic cardiomyopathy, although the role of Cav3 in Long QT syndrome has recently been disputed. Caveolin ...
Introduction: Caveolins (Cav), the principal structural proteins of the caveolar domain, have been implicated in the development of cardiac hypertrophy, pulmonary hypertension (PH) and lung remodeling. Mice with homozygous deletion of the Cav-1 gene display cardiac hypertrophy and pulmonary abnormalities characterized by thickened alveolar septa, hypercellularity and PH. In vivo administration of a cell-permeable Cav-1 mimetic peptide was previously shown to prevent the development of monocrotaline-induced pulmonary artery medial hypertrophy, PH and right ventricular hypertrophy in rats. Whether administration of such a Cav-1 peptide could rescue the cardio-pulmonary defects observed in Cav-1 KO mice remains unknown.. Methods and Results: Three week-old wild-type and Cav-1 KO mice were randomly assigned to receive a daily intraperitoneal injection of either saline, penetratin alone (AP, 2.5 mg/kg/d) or a peptide consisting of the Cav-1 scaffolding domain coupled to penetratin (AP-Cav, 2.5 ...
Caveolin-1 (Cav-1) is a 21?kDa protein enriched in caveolae and continues to be implicated in oncogenic cell transformation CHC tumorigenesis and metastasis. response to chemotherapy and radiation and tumor growth. We found Cav-1 is definitely overexpressed in human being Personal computer cell lines mouse models and human being pancreatic tumors and is associated with worse tumor grade and clinical results. In Personal computer cell lines disruption/depletion of caveolae/Cav-1 reduces proliferation colony formation and invasion. Radiation and chemotherapy up-regulate Cav-1 manifestation while Cav-1 depletion induces both chemosensitization and radiosensitization through modified apoptotic and DNA restoration signaling. and and transgene (KPC mouse) (Fig. 1B). Cav-1 manifestation was also tested on a cells microarray of 110 individuals with pancreatic malignancy and obtained semi-quantitatively for low versus high CHC manifestation. While Cav-1 is definitely virtually absent in pancreatic ...
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TY - JOUR. T1 - Caveolin-2 is targeted to lipid droplets, a new membrane domain in the cell. AU - Fujimoto, Toyoshi. AU - Kogo, Hiroshi. AU - Ishiguro, Kimiko. AU - Tauchi, Kumi. AU - Nomura, Ryuji. PY - 2001/3/5. Y1 - 2001/3/5. N2 - Caveolin-1 and -2 constitute a framework of caveolae in nonmuscle cells. In the present study, we showed that caveolin-2, especially its β isoform, is targeted to the surface of lipid droplets (LD) by immunofluorescence and immunoelectron microscopy, and by subcellular fractionation. Brefeldin A treatment induced further accumulation of caveolin-2 along with caveolin-1 in LD. Analysis of mouse caveolin-2 deletion mutants revealed that the central hydrophobic domain (residues 87-119) and the NH2-terminal (residues 70-86) and COOH-terminal (residues 120-150) hydrophilic domains are all necessary for the localization in LD. The NH2- and COOH-terminal domains appeared to be related to membrane binding and exit from ER, respectively, implying that caveolin-2 is ...
where to get vector overexpressing caveolin-1? - posted in Molecular Cloning: Trying to overexpress caveolin-1 in human cancer cells...are there readymade products out there which already has cav-1 attached to a dependable promoter? If so, where should I look? Thanks in advance!
Proteins and other substances can cross the endothelial layer that lines a blood vessel via two routes. Caveolin-1 is essential for both, Siddiqui et al. show.. Researchers already knew that caveolin-1 was necessary for transcellular protein trafficking, in which macromolecules such as albumin enter an endothelial cell from the bloodstream and exit on the tissue side. Caveolae swallow these molecular travelers and bundle them into vesicles that wend through the cell. In an alternative pathway, known as the paracellular route, molecules slip between the cells of the endothelial layer, passing through the adherens junctions that fasten adjacent cells together. Previous work showed that adherens junctions become permeable in mice lacking caveolin-1, suggesting that the protein helps seal the junctions.. Siddiqui et al. dissected the molecular chain of events that connects caveolin-1 to adherens junction integrity. Loss of caveolin-1 activated the enzyme eNOS, which spawns nitric oxide (NO) that ...
The scaffolding protein CAV1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to…
Limb-girdle muscular dystrophy (LGMD) is a rare heterogeneous group of human diseases. Besides the characteristic presentation of weakness in the proximal limbs and shoulder or pelvic girdles, the subtypes of LGMD do not share many features in common. The group is classified, by mode of inheritance, into LGMD1 (autosomal dominant) and LGMD2 (autosomal recessive). The forms are further sub grouped based on the causative gene loci. Spontaneous animal models of LGMD are uncommon and the majority of models are in transgenic mice. Since mutations that result in LGMD sometimes result in other phenotypes, many of these animal models are useful beyond the scope of LGMD. In general the animal models of LGMD do not closely resemble the human phenotype, with a few exceptions. Although many of these models do not exactly replicate the human disease, they are still valuable tools in biomedical research, not only to further studies in understanding the mechanisms of the disease, but also to identify and test ...
TY - JOUR. T1 - AMP-dependent kinase inhibits oxidative stress-induced caveolin-1 phosphorylation and endocytosis by suppressing the dissociation between c-Abl and Prdx1 proteins in endothelial cells. AU - Takeuchi, Kimio. AU - Morizane, Yuki. AU - Kamami-Levy, Cynthia. AU - Suzuki, Jun. AU - Kayama, Maki. AU - Cai, Wenyi. AU - Miller, Joan W.. AU - Vavvas, Demetrios G.. PY - 2013/7/12. Y1 - 2013/7/12. N2 - Caveolin-1 is the primary structural component of endothelial caveolae that is essential for transcellular trafficking of albumin and is also a critical scaffolding protein that regulates the activity of signaling molecules in caveolae. Phosphorylation of caveolin-1 plays a fundamental role in the mechanism of oxidant-induced vascular hyper permeability. However, the regulatory mechanism of caveolin-1 phosphorylation remains unclear. Here we identify a previously unexpected role for AMPKin inhibition of caveolin-1 phosphorylation under oxidative stress. A pharmacological activator of AMPK, ...
Lipids are the energy source used during liver regeneration. The research group, led by Dr. Albert Pol and with members from IDIBAPS, Universitat de Barcelona and Queensland University, unveils the essential role of the protein caveolin-1 in a fundamental process for liver cure after injury or transplant. Results also evidence the existence of cellular mechanisms by which excessive accumulation of lipids in the liver is a risk factor for the apparition of hepatic tumours.
Caveolae are specialised forms of lipid rafts in the plasma membrane of most cells. They form dynamic assemblies of sphingolipids and cholesterol containing scaffolding domains with different affinities for proteins resulting in a variety of functions [1]. The constitutive Cav-1 protein is distributed ubiquitously, while Cav-2 is usually associated with Cav-1 [3, 28]. Although Cav-3 is thought to be muscle specific and is expressed in striated muscles [3, 28, 29], we and others have found that Cav-3 is not expressed within human ASM [6, 30]. Recent studies have established that ASM caveolae contain a number of proteins important to [Ca2+]i regulation (e.g. agonist receptors, Ca2+ influx channels, and force regulatory proteins such as RhoA). In canine ASM, it has been established that caveolar-enriched membrane fractions express Cav-1, L-type Ca2+ channels and plasma membrane Ca2+ ATPase, but not SR proteins such as IP3R or RyR channels [31].. In ASM of different species, in addition to Ca2+ ...
Dysferlinopathies are a group of progressive muscular dystrophies characterized by mutations in the gene DYSF. These mutations cause scarcity or complete absence of dysferlin, a protein that is expressed in skeletal muscle and plays a role in membrane repair. Our objective was to unravel the protein …
Garg V, Sun W, Hu K (2009). "Caveolin-3 negatively regulates recombinant cardiac K(ATP) channels". Biochem. Biophys. Res. ... 2008). "Protein kinase C-epsilon induces caveolin-dependent internalization of vascular adenosine 5'-triphosphate-sensitive K+ ... 385 (3): 472-7. doi:10.1016/j.bbrc.2009.05.100. PMID 19481058. ABCC9 human gene location in the UCSC Genome Browser. ABCC9 ... 52 (3): 499-506. doi:10.1161/HYPERTENSIONAHA.108.110817. PMID 18663158. Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a ...
... has been shown to interact with Caveolin 3 in skeletal muscle, and this interaction is thought to retain dysferlin ... Hernández-Deviez DJ, Howes MT, Laval SH, Bushby K, Hancock JF, Parton RG (2008). "Caveolin regulates endocytosis of the muscle ... Dysferlin also interacts with MG53, and a functional interaction between dysferlin, caveolin-3 and MG53 is thought to be ... caveolin-3, and dysferlin". J. Biol. Chem. 284 (23): 15894-902. doi:10.1074/jbc.M109.009589. PMC 2708885. PMID 19380584. ...
Scherer PE, Lisanti MP (Aug 1997). "Association of phosphofructokinase-M with caveolin-3 in differentiated skeletal myotubes. ... 63 (3): 1154-65. doi:10.2337/db13-1301. PMC 3931395. PMID 24306210. Su Y, Zhou A, Al-Lamki RS, Karet FE (May 2003). "The a- ... 61 (3): 415-9. doi:10.1016/0009-8981(75)90434-9. PMID 125160. Zhao ZZ, Malencik DA, Anderson SR (Feb 1991). "Protein-induced ...
LQT9 is caused by variants in the membrane structural protein, caveolin-3. Caveolins form specific membrane domains called ... Similar to LQT3, these caveolin variants increase the late sustained sodium current, which impairs cellular repolarization. ...
LQT9 is caused by variants in the membrane structural protein, caveolin-3. Caveolins form specific membrane domains called ... Similar to LQT3, these caveolin variants increase the late sustained sodium current, which impairs cellular repolarization. ... 24 (1): 3-13. doi:10.1016/j.spen.2017.01.001. PMID 28779863. Syncope can lead to convulsions and can easily be confused with ... Both sets of guidelines agree that LQTS can also be diagnosed if an individual has a Schwartz score of greater than 3 or if a ...
... has been shown to interact with FYN, C-src tyrosine kinase, Src, NCK1, Grb2, Caveolin 3 and SHC1. Actin-binding ... Identification of a central WW-like domain within caveolin family members". The Journal of Biological Chemistry. 275 (48): ... "Caveolin-3 directly interacts with the C-terminal tail of beta -dystroglycan. ... 23 (3): 338-42. doi:10.1038/15519. PMID 10610181. S2CID 564897. Rentschler S, Linn H, Deininger K, Bedford MT, Espanel X, Sudol ...
IGFBP-3 is one of six IGF binding proteins (IGFBP-1 to IGFBP-6) that have highly conserved structures and bind the insulin-like ... The main IGFBP-3 ligands in the circulation are IGF-1 and IGF-2, and the acid-labile subunit (ALS). The serum proteins ... IGFBP-3 exerts antiproliferative effects in many cell types by blocking the ability of IGF-1 and IGF-2 to activate the IGF1R ( ... Since IGFBP-3 is abundant in the bloodstream of healthy adults (typically 2-4 mg/L), and is largely stabilized by its complex ...
"Interaction of synthetic peptides corresponding to the scaffolding domain of Caveolin-3 with model membranes". Biopolymers. 84 ... 23 (3): 413-418. doi:10.1016/S0196-9781(01)00628-3. PMID 11835989. Pallavi, B.; Nagaraj, R. (2003). "Palmitoylated peptides ...
Zhao G, Simpson RU (2010). "Membrane Localization, Caveolin-3 Association and Rapid Actions of Vitamin D Receptor in Cardiac ... 49 (3): 668-73. PMC 1683124. PMID 1652893. Szpirer J, Szpirer C, Riviere M, Levan G, Marynen P, Cassiman JJ, Wiese R, DeLuca HF ... 60 Suppl 3: 106-10. PMID 11979895. Uitterlinden AG, Fang Y, Van Meurs JB, Pols HA, Van Leeuwen JP (2004). "Genetics and biology ... 3 (3): 361-70. doi:10.1016/S1097-2765(00)80463-3. PMID 10198638. Tagami T, Lutz WH, Kumar R, Jameson JL (December 1998). "The ...
... has been shown to interact with: ARF1, Aldolase A, Amphiphysin, BIN1, Caveolin 1, Glyceraldehyde 3-phosphate dehydrogenase ... functional interaction with caveolin in low-density membrane microdomains". FEBS Letters. 467 (2-3): 326-32. doi:10.1016/s0014- ... functional interaction with caveolin in low-density membrane microdomains". FEBS Letters. 467 (2-3): 326-32. doi:10.1016/S0014- ... N-(2-(1-(3-fluorophenyl)-4-oxo-1,3,8-triazaspiro[4.5]decan-8-yl)ethyl)-2-naphthamide: 75-fold selective versus PLD1, IC50 = 20 ...
2005). "Junctophilin type 2 is associated with caveolin-3 and is down-regulated in the hypertrophic and dilated ... 325 (3): 852-6. doi:10.1016/j.bbrc.2004.10.107. PMID 15541368. Kim J, Bhinge AA, Morgan XC, Iyer VR (2005). "Mapping DNA- ... 273 (3): 920-7. doi:10.1006/bbrc.2000.3011. PMID 10891348. Garbino A, van Oort RJ, et al. (2009). "Molecular evolution of the ... 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. Matsushita Y, Furukawa T, Kasanuki H, et al. ( ...
G-protein-coupled receptor signaling components localize in both sarcolemmal and intracellular caveolin-3-associated ... 5(3):237-48. García-Marcos, M., Ghosh, P., and M.G. Farquhar. 2009. GIV is a non-receptor GEF factor for Galphai with a unique ... Retrieved 3 May 2019. CS1 maint: discouraged parameter (link) Shearer, B. F.; Shearer, B. S., eds. (1996). Notable women in the ... 2006 Jul 3; PMID 16818493 Farquhar, M. G. 2006. The glomerular basement membrane: not gone, just forgotten. J. Clin. Invest. ...
Llorente A, de Marco MC, Alonso MA (Oct 2004). "Caveolin-1 and MAL are located on prostasomes secreted by the prostate cancer ... Millán J, Puertollano R, Fan L, Alonso MA (Apr 1997). "Caveolin and MAL, two protein components of internal detergent-insoluble ... 233 (3): 707-12. doi:10.1006/bbrc.1997.6530. PMID 9168919. Martín-Belmonte F, Kremer L, Albar JP, Marazuela M, Alonso MA (Apr ... 82 (3): 550-62. doi:10.1046/j.1471-4159.2002.00987.x. PMID 12153479. S2CID 39619359. Copie-Bergman C, Plonquet A, Alonso MA, ...
Llorente A, de Marco MC, Alonso MA (2005). "Caveolin-1 and MAL are located on prostasomes secreted by the prostate cancer PC-3 ... 19 (3): 925-33. PMID 15168355. Rohan S, Tu JJ, Kao J, et al. (2007). "Gene expression profiling separates chromophobe renal ... 76 (1-3): 81-8. doi:10.1006/geno.2001.6610. PMID 11549320. "Entrez Gene: MAL2 mal, T-cell differentiation protein 2". Marazuela ... 332 (3): 675-87. doi:10.1016/S0022-2836(03)00944-6. PMID 12963375. Strausberg RL, Feingold EA, Grouse LH, et al. (2003). " ...
The C-terminus of DLC1 is also known to interact with caveolin-1, although the biological significance of this interaction has ... caveolin-1, DLC-1, and NM23-H2 as putative antitumorigenic, progesterone-regulated genes for ovarian cancer cells by gene ... The detailed pathways by which DLC1 results in the cleavage of procaspase-3 and decrease in Bcl-2 levels require further ... DLC1 is responsible for inducing programmed cell death by at least two mechanisms: caspase-3-mediated apoptosis and Bcl-2 ...
... caveolin 1 MeSH D12.776.543.990.100.750 - caveolin 2 MeSH D12.776.543.990.100.875 - caveolin 3 MeSH D12.776.543.990.150.100 - ... toll-like receptor 3 MeSH D12.776.543.750.705.910.500.400 - toll-like receptor 4 MeSH D12.776.543.750.705.910.500.500 - toll- ... type 3 MeSH D12.776.543.750.060.116 - receptor, fibroblast growth factor, type 4 MeSH D12.776.543.750.060.124 - proto-oncogene ... erbb-3 MeSH D12.776.543.750.060.468 - receptor, igf type 1 MeSH D12.776.543.750.060.484 - receptor, insulin MeSH D12.776. ...
Caveolin-3 Association and Rapid Actions of Vitamin D Receptor in Cardiac Myocytes". Steroids 75 (8-9): 555-9. PMC 2885558. ... doi:10.1016/S1097-2765(00)80463-3. *^ a b Tagami T, Lutz WH, Kumar R, Jameson JL (December 1998). "The interaction of the ... Sinteza receptora vitamina vitamina D3 je genetički kontrolrana. Ovaj receptor istovremeno funkcionira i kao sekundarna žučna i ... 3)-induced differentiation by sequestering the vitamin D(3) receptor". Cancer Res. 62 (23): 7050-8. PMID 12460926. ...
This gene codes for the Caveolin protein, which is a scaffolding membrane protein. This protein plays a role in lipid ... April 2008). "Association of a Homozygous Nonsense Caveolin-1 Mutation with Berardinelli-Seip Congenital Lipodystrophy". ... More recently, type 3 CGL was identified as a separate type of CGL, which was identified as a mutation in the CAV1 gene. Then, ... Types 3 and 4 are two different mutations but they share a common defective pathway. Medical diagnosis of CGL can be made after ...
There are two isoforms of caveolin-1: caveolin-1α and caveolin-1β, the latter lacking a part of the N-terminus. Caveolins are ... The expression pattern of caveolin-2 is similar to that of caveolin-1; it seems to be co-expressed with caveolin-1. The ... The caveolin gene family has three members in vertebrates: CAV1, CAV2, and CAV3, coding for the proteins caveolin-1, caveolin-2 ... Caveolin-1 has also been shown to play a role in the integrin signaling. The tyrosine phosphorylated form of caveolin-1 ...
... within caveolin-1 molecule. Molecules that interact with caveolin-1 contain caveolin-binding motifs (CBM). Caveolin GRCh38: ... Fra AM, Mastroianni N, Mancini M, Pasqualetto E, Sitia R (March 1999). "Human caveolin-1 and caveolin-2 are closely linked ... caveolin 2, PLD2, Bruton's tyrosine kinase, and SCP2. All these interactions are through a caveolin-scaffolding domain (CSD) ... caveolin. Caveolin binding negatively regulates the auto-activation of Src tyrosine kinases". The Journal of Biological ...
Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting ... "Entrez Gene: CAV3 caveolin 3". Hedley PL, Kanters JK, Dembic M, Jespersen T, Skibsbye L, Aidt FH, Eschen O, Graff C, Behr ER, ... Caveolin-3 is a protein that in humans is encoded by the CAV3 gene. Alternative splicing has been identified for this locus, ... Caveolin 3 has been shown to interact with a range of different proteins, including, but not limited to: DAG1, DYSF, EGFR, and ...
... has been shown to interact with Caveolin 1 and RAS p21 protein activator 1. GRCh38: Ensembl release 89: ... 1998). "Expression of caveolin-1 and -2 in differentiating PC12 cells and dorsal root ganglion neurons: caveolin-2 is up- ... Fra AM, Mastroianni N, Mancini M, Pasqualetto E, Sitia R (May 1999). "Human caveolin-1 and caveolin-2 are closely linked genes ... 2002). "Epithelial expression of caveolin-2, but not caveolin-1, is enhanced in the inflamed mucosa of patients with ulcerative ...
Caveolin-3 Association and Rapid Actions of Vitamin D Receptor in Cardiac Myocytes". Steroids 75 (8-9): 555-9. PMC 2885558. ... 11,0 11,1 11,2 11,3 11,4 Kitagawa H, Fujiki R, Yoshimura K, Mezaki Y, Uematsu Y, Matsui D, Ogawa S, Unno K, Okubo M, Tokita A, ... doi:10.1016/S1097-2765(00)80463-3.. *↑ 14,0 14,1 Tagami T, Lutz WH, Kumar R, Jameson JL (December 1998). "The interaction of ... Genet. 49 (3): 668-73. PMC 1683124. PMID 1652893.. *. Szpirer J, Szpirer C, Riviere M, Levan G, Marynen P, Cassiman JJ, Wiese R ...
... co-immunoprecipitates with src kinases and caveolin". Pancreas. 21 (3): 219-25. doi:10.1097/00006676-200010000-00001. PMID ... 1491 (1-3): 376-80. doi:10.1016/S0167-4781(00)00057-9. PMID 10760606. Parker EM, Zaman MM, Freedman SD (2001). "GP2, a GPI- ... J. 277 (3): 879-81. doi:10.1042/bj2770879. PMC 1151326. PMID 1651706. Fukuoka S, Freedman SD, Scheele GA (1991). "A single gene ... 79 (3-4): 231-2. doi:10.1159/000134730. PMID 9605860. "Entrez Gene: GP2 glycoprotein 2 (zymogen granule membrane)". Jacob M, ...
Caveolin-1 Influences LFA-1 Redistribution upon TCR Stimulation in CD8 T Cells (2017) Proximity of TCR and its CD8 coreceptor ... "Caveolin-1 Influences LFA-1 Redistribution upon TCR Stimulation in CD8 T Cells". Journal of Immunology. 199 (3): 874-884. doi: ... investigating the role of Caveolin-1 in T cell cholesterol homeostasis, integrin signaling and exosome complex biogenesis. In ...
Disruption of the maxi-K-caveolin-1 interaction alters current expression in human myometrial cells. Reprod Biol Endocrinol. ... 2019;34(3):323-331. doi:10.1177/0748730419844650 Reschke L, McCarthy R, Herzog ED, Fay JC, Jungheim ES, England SK. ... Funded by the March of the Dimes, England serves as Associate Program Director and Theme 3 Leader of the Prematurity Research ... 2002;192(1-2):1-6. doi:10.1016/s0303-7207(02)00136-3 Benkusky NA, Fergus DJ, Zucchero TM, England SK. Regulation of the Ca2+- ...
Caveolin-1) Associated With Human Pulmonary Arterial Hypertension". Circulation: Cardiovascular Genetics. 5 (3): 336-343. doi: ... 132 (3): 285-292. doi:10.1007/s00439-012-1249-0. ISSN 1432-1203. PMC 3570587. PMID 23138528. Austin Eric D.; Ma Lijiang; LeDuc ... 97 (3): 457-464. doi:10.1016/j.ajhg.2015.07.014. ISSN 0002-9297. PMC 4564988. PMID 26299366. Cheung, Yee Him; Gayden, Tenzin; ... 51 (3): 197-202. doi:10.1136/jmedgenet-2013-101989. ISSN 1468-6244. PMC 3955383. PMID 24385578. Chung, Wendy K.; Arkovitz, Marc ...
Caveolin 1 is dephosphorylated on tyrosine 14 in response to shear stress and PTPmu is hypothesized to catalyze this reaction. ... Caveolin 1 is a scaffolding protein enriched in endothelial cell junctions that is also linked to shear stress regulated ... Shin J, Jo H, Park H (2006). "Caveolin-1 is transiently dephosphorylated by shear stress-activated protein tyrosine phosphatase ... 34 (3): 453-67. doi:10.1016/j.mcn.2006.11.022. PMC 185529. PMID 17234431. Phillips-Mason PJ, Kaur H, Burden-Gulley SM, Craig SE ...
RhoC GTPase is overexpressed, possibly related to overexpression (hypomethylation) of caveolin-1 and -2. Caveolin is ... caveolin-1 and -2 are overexpressed and may contribute to tumour cell motility[13] ... "Overexpression of caveolin-1 and -2 in cell lines and in human samples of inflammatory breast cancer". Breast Cancer Research ... ISBN 978-3-0348-0836-1.. *^ "Facts for Life - Inflammatory Breast Cancer" (PDF). Susan G. Komen for the Cure. Retrieved 2006-12 ...
Feng X, Gaeta ML, Madge LA, Yang JH, Bradley JR, Pober JS (March 2001). "Caveolin-1 associates with TRAF2 to form a complex ... Caveolin 1, EDARADD, HIVEP3, IKK2, Low affinity nerve growth factor receptor, MAP3K14, MAP3K1, MAP3K7IP2, MAP4K2, MAP4K5, RANK ... "A phosphotyrosine-dependent protein interaction screen reveals a role for phosphorylation of caveolin-1 on tyrosine 14: ... 2 (3): 389-95. doi:10.1016/s1097-2765(00)80283-x. PMID 9774977. Hoeflich KP, Yeh WC, Yao Z, Mak TW, Woodgett JR (October 1999 ...
978-3-540-13679-8. . PMID 6240757.. *^ Vallbo AB, al-Falahe NA (February 1990). "Human muscle spindle response in a motor ... 3) Fusimotor template of intended movement. Static gamma activity is a "temporal template" of the expected shortening and ... 571 (Pt 3): 711-23. doi:10.1113/jphysiol.2005.101634. PMC 1805796. PMID 16423858.. ...
Yamamoto H, Komekado H, Kikuchi A (2006). "Caveolin is necessary for Wnt-3a-dependent internalization of LRP6 and accumulation ...
Two examples of adaptor proteins are AP180[3] and epsin.[4][5][6] AP180 is used in synaptic vesicle formation. It recruits ... doi:10.1016/S0968-0004(02)02089-3. PMID 12076538. Archived (PDF) from the original on 2008-11-21.. ... Issue 3, 471-484, 5 August 2011 Abstract Archived 2012-01-19 at the Wayback Machine. ...
"Amyloid β-Protein Stimulates Trafficking of Cholesterol and Caveolin-1 from the Plasma Membrane to the Golgi Complex in Mouse ... Med. 3 (9): 1016-20. PMID 9288729. doi:10.1038/nm0997-1016.. *↑ Yin YI, Bassit B, Zhu L, Yang X, Wang C, Li YM (August 2007). " ... 62,0 62,1 62,2 62,3 62,4 Kang JE, Lim MM, Bateman RJ, Lee JJ, Smyth LP, Cirrito JR, Fujiki N, Nishino S, Holtzman DM (November ... Neurol. 38 (3): 421-8. PMID 7668828. doi:10.1002/ana.410380312.. *↑ Kamal A, Almenar-Queralt A, LeBlanc JF, Roberts EA, ...
... is a sheath of connective tissue that groups muscle fibers into bundles (anywhere between 10 and 100 or more) or fascicles. Studies of muscle physiology suggest that the perimysium plays a role in transmitting lateral contractile movements. This hypothesis is strongly supported in one exhibition of the existence of "perimysial junctional plates" in ungulate flexor carpi radialis muscles constructed by Emilie Passerieux.[1] The overall comprehensive organization of the perimysium collagen network, as well as its continuity and disparateness, however, have still not been observed and described thoroughly everywhere within the muscle. Found to have type I, III, VI, and XII collagen. ...
Muscle contraction consists of the simultaneous shortening of multiple sarcomeres.[3] Muscle fiber contraction[edit]. The axon ...
... caveolin, dynamin and actin. Once within the cell the viruses are rapidly delivered to endosomes via vesicular trafficking ... The 5' and 3' terminal 19 nt viral promoter regions of both RNA segments are necessary for recognition and binding of the viral ... but it also suppresses host innate IFN response by inhibiting translocation of IRF-3. NP of Lassa virus is reported to have an ... 3] There are no approved vaccines against Lassa fever for use in humans.[4] ...
3) The vesicle is transported to the active zone and docked in close proximity to the plasma membrane. (4) During an action ... 3] The glutamate synapse contains smaller pyramid like structures that extend about 50 nm from the membrane.[4] The ...
... localizes in a lipid raft/caveola and inhibits ERK activation in collaboration with caveolin-1". Genes to Cells. 10 (9): 887-95 ... 283 (3): 1679-91. doi:10.1074/jbc.M705457200. PMID 17974561.. *. Johne C, Matenia D, Li XY, Timm T, Balusamy K, Mandelkow EM ( ... An exon 3 c.46C,T mutation leading to p.Arg16Stop.[8] This mutation may result in a truncated nonfunctional protein. Blast ... "Spred-1 negatively regulates interleukin-3-mediated ERK/mitogen-activated protein (MAP) kinase activation in hematopoietic ...
Heritable (BMPR2, ALK1, SMAD9, caveolin 1, KCNK3 mutations). *Drug- and toxin-induced (e.g., methamphetamine use [23] ) ... 62 (3): 525-63. doi:10.1124/pr.110.002907. PMC 2964902. PMID 20716671.. CS1 maint: multiple names: authors list (link) ... 3 Pathogenesis *3.1 Molecular pathology *3.1.1 Nitric oxide-soluble guanylate cyclase pathway ... Since it has a half-life of 3 to 5 minutes, the infusion has to be continuous, and interruption can be fatal.[72] Other ...
Yamamoto, M; Toya Y; Jensen R A; Ishikawa Y (March 1999). "Caveolin is an inhibitor of platelet-derived growth factor receptor ... B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7, 3.1.3.48, 3.4.21/22/23/24, ... PDGFRA formira interakcije sa PDGFRB,[2][3] PLCG1,[4] Natrijum-vodonik antiporter 3 regulator 1,[5] Cbl gene,[6] CRK,[7][8] ...
Vargas L, Nore BF, Berglof A, Heinonen JE, Mattsson PT, Smith CI, Mohamed AJ (March 2002). "Functional interaction of caveolin- ... phosphatidylinositol-3,4,5-trisphosphate binding. • non-membrane spanning protein tyrosine kinase activity. • ATP binding. • ... 1bwn: PH DOMAIN AND BTK MOTIF FROM BRUTON'S TYROSINE KINASE MUTANT E41K IN COMPLEX WITH INS(1,3,4,5)P4 ... Btk contains a PH domain that binds phosphatidylinositol (3,4,5)-trisphosphate (PIP3). PIP3 binding induces Btk to ...
... s are skeletal muscle fibers that serve as specialized sensory organs (proprioceptors) that detect the amount and rate of change in length of a muscle.[1] They constitute the muscle spindle and are innervated by both sensory (afferent) and motor (efferent) fibers. Gamma efferents from small multipolar cells from anterior gray column innervate it. These form a part of neuromuscular spindles. Intrafusal muscle fibers are walled off from the rest of the muscle by an outer connective tissue sheath consisting of flattened fibroblasts and collagen.[2] This sheath has a spindle or "fusiform" shape, hence the name "intrafusal". There are two types of intrafusal muscle fibers: nuclear bag and nuclear chain fibers. They bear two types of sensory ending, known as annulospiral and flower-spray endings. Both ends of these fibers contract but the central region only stretches and does not contract. They are innervated by gamma motor neurons and beta motor neurons. It is by the sensory ...
Although receptors and their ligands can be brought into the cell through a few mechanisms (e.g. caveolin), clathrin-mediated ... 3] The activated receptor becomes internalised and is transported to late endosomes and lysosomes for degradation. However, ...
Frank P, Lisanti M (2004). "Caveolin-1 and caveolae in atherosclerosis: differential roles in fatty streak formation and ... Li X, Everson W, Smart E (2005). "Caveolae, lipid rafts, and vascular disease". Trends Cardiovasc Med 15 (3): 92-6. PMID ...
The other component, orthogonal to the direction of action of the muscle (Orthogonal force = Total force × sinΦ) is not exerted on the tendon, but simply squeezes the muscle, by pulling its aponeuroses toward each other. Notice that, although it is practically convenient to compute PCSA based on volume or mass and fiber length, PCSA (and therefore the total fiber force, which is proportional to PCSA) is not proportional to muscle mass or fiber length alone. Namely, the maximum (tetanic) force of a muscle fiber simply depends on its thickness (cross-section area) and type. By no means it depends on its mass or length alone. For instance, when muscle mass increases due to physical development during childhood, this may be only due to an increase in length of the muscle fibers, with no change in fiber thickness (PCSA) or fiber type. In this case, an increase in mass does not produce an increase in force. Sometimes, the increase in mass is associated with an increase in thickness. Only in this ...
396 (Pt 1): e1-3. doi:10.1042/BJ20060377. ISSN 0264-6021. PMC 1450001. PMID 16626281.. ... 3] They are found in ventricular muscle cells in most species, and in atrial muscle cells from large mammals.[4] In cardiac ... there are 3 times as many L-type calcium channels located within the T-tubule membrane compared to the rest of the sarcolemma. ...
doi:10.1016/0022-510x(73)90023-3. PMID 4120482.. *^ Michael Yessis (2006). Build A Better Athlete. Ultimate Athlete Concepts. ... 106 (3): 463-475. doi:10.1016/0305-0491(93)90120-T.. *^ Buchthal, F; Schmalbruch, H (Aug 1970). "Contraction times and fibre ... The striated cells of cardiac and skeletal muscles are referred to as muscle fibers.[3] Cardiomyocytes are the muscle fibres ... glycolytic system for energy transfer and can contract and develop tension at 2-3 times the rate of slow twitch fibers. Fast ...
Caveolin 1. *Caveolin 3. *Collagen, type I, alpha 2. *Complement component 1r ...
Lu, Zhimin; Ghosh, Sourav; Wang, Zhiyong; Hunter, Tony (December 2003). "Downregulation of caveolin-1 function by EGF leads to ... 233 (3): 706-20. doi:10.1002/dvdy.20345. PMID 15937929.. *^ Kerosuo L, Bronner-Fraser M (2012). "What is bad in cancer is good ... 4 (3): 380-382. doi:10.4161/cc.4.3.1538. PMID 15711124.. *^ Atouf F, Park CH, Pechhold K, et al. (2007). "No evidence for mouse ... 15 (3): 178-196. doi:10.1038/nrm3758. PMC 4240281. PMID 24556840.. *^ Thiery JP, Acloque H, Huang YJ, Nieto MA (2009). " ...
ISBN 0-7216-9549-3.. *^ Quoted from National Skeletal Muscle Research Center; UCSD, Muscle Physiology Home Page - Skeletal ... 9 (3): e91754. Bibcode:2014PLoSO...991754M. doi:10.1371/journal.pone.0091754. PMC 3951451. PMID 24622330.. ... Together, two terminal cisternae and a transverse tubule form a triad.[3] ... 3. pp. 1337-62. doi:10.1002/cphy.c120033. ISBN 9780470650714. PMID 23897689.. ...
978-3-527-30594-0. .. *^ Reddy AS, Day IS (2001). "Analysis of the myosins encoded in the recently completed Arabidopsis ... 30 (3): 389-411. doi:10.1007/BF02738121. PMID 10403058. S2CID 13319819.. *Molecular Biology of the Cell. Alberts, Johnson, ... However, beginning in the 1970s, researchers began to discover new myosin genes in simple eukaryotes[3] encoding proteins that ... 3] Following the discovery by Pollard and Korn (1973) of enzymes with myosin-like function in Acanthamoeba castellanii, a ...
12 (3): 160-168. doi:10.1007/bf00289169. PMID 4712973.. *^ a b Burke RE, Levine DN, Tsairis P, Zajac FE (November 1973). " ... 285 (3): R570-80. doi:10.1152/ajpregu.00646.2002. PMID 12738613.. *^ Baldwin KM, Haddad F (January 2001). "Effects of different ... 3] Henneman's size principle indicates that motor units are recruited from smallest to largest based on the size of the load. ...
... caveolin 3) ... 相关:一氧化氮合成酶1(英语:NOS1) · 小窝蛋白3(英语: ... 维基媒体基金会是按美国国內稅收法
doi:10.1007/s10072-015-2399-3. PMID 26482054.. *^ Muhammad A, Flores I, Zhang H, Yu R, Staniszewski A, Planel E, Herman M, Ho L ...
3)K function to Rab5 regulation of endosome fusion". Nature. 394 (6692): 494-8. doi:10.1038/28879. PMID 9697774.. ...
Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting ... "Entrez Gene: CAV3 caveolin 3". Hedley PL, Kanters JK, Dembic M, Jespersen T, Skibsbye L, Aidt FH, Eschen O, Graff C, Behr ER, ... Caveolin-3 is a protein that in humans is encoded by the CAV3 gene. Alternative splicing has been identified for this locus, ... Caveolin 3 has been shown to interact with a range of different proteins, including, but not limited to: DAG1, DYSF, EGFR, and ...
Goat polyclonal Caveolin-3 antibody. Validated in WB, IHC and tested in Mouse, Rat, Human, Pig. Immunogen corresponding to ... Primary - Goat Anti-Caveolin-3 antibody (ab87770) WB, IHC-P Secondary - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129) ... All lanes : Anti-Caveolin-3 antibody (ab87770) at 0.1 µg/ml. Lane 1 : pig skeletal muscle tissue lysate. Lane 2 : pig heart ... All lanes : Anti-Caveolin-3 antibody (ab87770) at 0.3 µg/ml. Lane 1 : mouse heart tissue lysate. Lane 2 : rat heart tissue ...
Abcam provides specific protocols for Anti-Caveolin-3 antibody (ab87770) : Western blot protocols, Immunohistochemistry ...
... caveolin-1α and -1β, caveolin-2, and caveolin-3). Caveolin-1 and -2 are coexpressed and abundantly expressed in adipocytes, ... Caveolin-mediated regulation of signaling along the p42/44 MAP kinase cascade in vivo: a role for the caveolin-scaffolding ... Several caveolin-related proteins have been shown to bind to caveolins through caveolin-binding domains (øXøXXXXø or øXXXXøXXø ... and found that they did not have caveolin-binding domains. This suggests that JNK is not regulated by caveolin. ...
Identification, sequence, and expression of caveolin-2 defines a caveolin gene family. Proc. Natl. Acad. Sci. USA 93, 131-135 ( ... Aberrant dysferlin trafficking in cells lacking caveolin or expressing dystrophy mutants of caveolin-3. Hum. Mol. Genet. 15, ... Way, M. & Parton, G. M-caveolin, a muscle-specific caveolin-related protein. FEBS Lett. 376, 108-112 (1995). ... Byrne, D. P., Dart, C. & Rigden, D. J. Evaluating caveolin interactions: do proteins interact with the caveolin scaffolding ...
Tags: Caveolin-3, Duchenne Muscular Dystrophy, Dystrophin, Jerry Lewis, Muscular Dystrophy Association, Niemann Pick Type C, ... You are here: Home / Archives for Caveolin-3. Calling Jerry Lewis - Scientists Report Understanding Niemann Pick Type C Could ...
The Caveolin-3 is purified by proprietary chromatographic techniques. ... Recombinant Human Caveolin-3 full length protein expressed in E.coli, shows a 50 kDa band on SDS-PAGE (Including GST). ... Three proteins, caveolin-1, caveolin-2 and caveolin-3 have been identified as principle components of caveolae.. ... Recombinant Human caveolin 3. Download Datasheet See All CAV3 Products. Bring this labeled protein directly to your bench! ...
Abstract 21013: Caveolin 3-Containing Lipid Rafts Synergized With Insulin to Activate Akt Kinase by Blocking Akt ... Abstract 21013: Caveolin 3-Containing Lipid Rafts Synergized With Insulin to Activate Akt Kinase by Blocking Akt ... Abstract 21013: Caveolin 3-Containing Lipid Rafts Synergized With Insulin to Activate Akt Kinase by Blocking Akt ... Abstract 21013: Caveolin 3-Containing Lipid Rafts Synergized With Insulin to Activate Akt Kinase by Blocking Akt ...
Previous experiments have demonstrated that membrane proteins interact with caveolin scaffolding domain via a specific caveolin ... Role of caveolin and caveolae in insulin signaling and diabetes. Am J Physiol Endocrinol Metab. 2003;285:E1151-E1160. ... Caveolin gene transfer improves glucose metabolism in diabetic mice. Am J Physiol Cell Physiol. 2010;298:C450-C456. ... Essential Role of Caveolin-3 in Adiponectin Signalsome Formation and Adiponectin Cardioprotection. Yajing Wang, Xiaoliang Wang ...
Abstract 15887: MURC/cavin-4 Functions as a Negative Regulator of Caveolin-3 in Tte Development of Heart Failure. Daisuke Naito ... Conclusion: MURC functions as a negative regulator of caveolin-3 at caveolae and modulates localization of caveolin-3 in CMs. ... MURC was also colocalized and associated with caveolin-3 at the plasma membrane of CMs, suggesting an inhibitory interaction of ... MURCΔCC impaired membrane localization of caveolin-3, resulting in increased cAMP levels in CMs compared with MURC. Transgenic ...
Several mutations in the muscle-specific caveolin, caveolin-3, lead to a form of autosomal dominant muscular dystrophy referred ... A caveolin-3 mutant that causes limb girdle muscular dystrophy type 1C disrupts Src localization and activity and induces ... Caveolin 3. Caveolins / genetics*, metabolism. Cells, Cultured. Membrane Microdomains / metabolism. Mice. Microscopy, ... The TFT mutation reduced the binding of Src to caveolin-3, diminished targeting of Src to lipid rafts, and caused abnormal ...
Conclusion: Overall, we show for the first time that a high palmitate diet leads to loss of caveolin-3 in cardiomyocytes and to ... To determine the mechanism of the loss of plasma membrane bound eNOS, we investigated the effect of palmitate on caveolin-3 and ... Ceramide is a sphingolipid and localizes to caveolae, which are lined in the inner membrane leaflet by caveolin proteins. In ... The remaining 30% of caveolin-3 was localized to a peri-nuclear location. In contrast to previous studies, palmitate did not ...
TRPC1 binds to caveolin-3 and is regulated by Src kinase - role in Duchenne muscular dystrophy ... TRPC1 binds to caveolin-3 and is regulated by Src kinase - role in Duchenne muscular dystrophy ... TRPC1 binds to caveolin-3 and is regulated by Src kinase - role in Duchenne muscular dystrophy ... TRPC1 binds to caveolin-3 and is regulated by Src kinase - role in Duchenne muscular dystrophy ...
The Role of CAV3 in Long-QT Syndrome Clinical and Functional Assessment of a Caveolin-3/Kv11.1 Double Heterozygote Versus ... The Role of CAV3 in Long-QT Syndrome Clinical and Functional Assessment of a Caveolin-3/Kv11.1 Double Heterozygote Versus ... The Role of CAV3 in Long-QT Syndrome Clinical and Functional Assessment of a Caveolin-3/Kv11.1 Double Heterozygote Versus ... Caveolin-3 Single Heterozygote. CIRCULATION-CARDIOVASCULAR GENETICS , 6 (5) pp. 452-461. 10.1161/CIRCGENETICS.113.000137. ...
A deficiency in caveolin-3 (Cav-3), the major striated muscle isoform, is responsible for skeletal muscle disorders, such as ... Here we show that a loss of Cav-3 induced by the expression of the LGMD 1C-associated mutant P104L (Cav-3(P104L)) provokes a ... In myotubes expressing Cav-3(P104L), the loss of Cav-3 was accompanied by a 66% reduction in Ca(v)1.1 mean labelling intensity ... Confocal immunomiscrocopy indicated a colocalization of Cav-3 and Ca(v)1.1, the pore-forming subunit of the L-type Ca(2+) ...
... signaling is dependent on caveolin-1; however, it is not known whether caveolin-1 is associated with the beta(3)-AR or solely ... Interaction with Caveolin-1 Modulates G Protein Coupling of Mouse beta(3)-Adrenoceptor. Sato, Masaaki Stockholm University, ... The endogenous beta(3a)-AR displayed G alpha(i/o) coupling in brown adipocytes from caveolin-1 knock-out mice or in wild type ... Our studies indicate that interaction of the beta(3a)-AR with caveolin inhibits coupling to G alpha(i/o) proteins and suggest ...
Caveolin-3 is a muscle specific scaffolding protein with both structural and signaling roles. Lack of caveolin-3 expression has ... In differentiated caveolin-3 null myotubes, agrin treatment yields a 60% reduction in nAChR clusters as compared to agrin ... Hezel, Michael P. (2009) LGMD-1C: Role of Caveolin-3 in Neuromuscular Junction Structure and Function. Doctoral Dissertation, ... Immunoprecipitation of MuSK shows that caveolin-3 and MuSK association peaks at 1 hour of agrin treatment in wildtype cells. ...
Caveolin-3 (CAV3) is a muscle-specific protein localized to the sarcolemma. It was suggested that CAV3 is involved in the ... p.P104L Caveolin-3 causes Golgi/ER stress. Mis-localization of CAV3 aggregates (formed by mutant and wild-type protein) to the ... Caveolin-3 T78M and T78K missense mutations lead to different phenotypes in vivo and in vitro. Lab Investig. 2008;88:275-83. ... Caveolin-3 plays a critical role in autophagy after ischemia-reperfusion. Am J Physiol Cell Physiol. 2016;311:C854-65.View ...
Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting ... The interaction of caveolin 3 protein with the potassium inward rectifier channel Kir2.1: physiology and pathology related to ... This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell ... Identification and functional analysis of a new putative caveolin-3 variant found in a patient with sudden unexplained death.. ...
Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting ... This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell ...
The caveolin gene family has three members in vertebrates: CAV1, CAV2, and CAV3, coding for the proteins caveolin-1, caveolin-2 ... There are two isoforms of caveolin-1: caveolin-1α and caveolin-1β, the latter lacking a part of the N-terminus. ... The expression pattern of caveolin-2 is similar to that of caveolin-1; it seems to be co-expressed with caveolin-1. ... Caveolin-1 has also been shown to play a role in the integrin signaling. The tyrosine phosphorylated form of caveolin-1 ...
Mammals have three caveolin proteins:caveolin-1 (Cav-1, or VIP21), caveolin-2 and caveolin-3 (or M-caveolin). Various classes ... Evaluating caveolin interactions: do proteins interact with the caveolin scaffolding domain through a widespread aromatic ... Caveolae require the caveolin protein for formation. Caveolins may act as scaffolding proteins within caveolar membranes by ...
Recombinant Human Caveolin-3 protein Full length protein datasheet (ab114264). Abcam offers quality products including ... Recombinant Human Caveolin-3 protein. Caveolin-3 タンパク質・ペプチド 製品一覧. ... お問い合わせは電話 +81-(0)3-6231-0940 または メー
Song KS, Scherer PE, Tang Z, Okamoto T, Li S, Chafel M, Chu C, Kohtz DS & Lisanti MP 1996 Expression of caveolin-3 in skeletal ... Caveolin expression levels in primary culture of FS cells after 72-h incubation. (A-C) The expression of caveolin 1, 2, and 3 ... Caveolin expression levels in primary culture of FS cells after 72-h incubation. (A-C) The expression of caveolin 1, 2, and 3 ... Caveolin expression levels in primary culture of FS cells after 72-h incubation. (A-C) The expression of caveolin 1, 2, and 3 ...
MURC/cavin-4 is co-expressed with Caveolin-3 in rhabdomyosarcoma tumors and its silencing prevents myogenic differentiation in ... MURC/cavin-4 is co-expressed with Caveolin-3 in rhabdomyosarcoma tumors and its silencing prevents myogenic differentiation in ... MURC/cavin-4 is co-expressed with Caveolin-3 in rhabdomyosarcoma tumors and its silencing prevents myogenic differentiation in ... MURC/cavin-4 is co-expressed with Caveolin-3 in rhabdomyosarcoma tumors and its silencing prevents myogenic differentiation in ...
Rabbit Monoclonal Anti-Caveolin-1 Antibody (SP43). Caveolae Marker, Endosome Marker. Validated: IHC, IHC-P. Tested Reactivity: ... The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle ... Caveolin-1 Antibody (SP43) Summary. Immunogen. Synthetic peptide corresponding to C-terminus of human Caveolin 1 protein ... Blogs on Caveolin-1. There are no specific blogs for Caveolin-1, but you can read our latest blog posts. ...
Mouse Monoclonal Anti-Caveolin-1 Antibody (7C8) [Allophycocyanin]. Caveolae Marker, Endosome Marker. Validated: Flow. Tested ... Reviews for Caveolin-1 Antibody (NB100-615APC) (0) There are no reviews for Caveolin-1 Antibody (NB100-615APC). By submitting a ... Blogs on Caveolin-1. There are no specific blogs for Caveolin-1, but you can read our latest blog posts. ... Diseases for Caveolin-1 Antibody (NB100-615APC). Discover more about diseases related to Caveolin-1 Antibody (NB100-615APC). ...
B) Caveolin expression in NIH v-Src cells, treated with siRNA against caveolin, was evaluated by western blotting of whole-cell ... The presence of caveolin negatively regulates bacterial internalization. (A) Wild-type (CavWT) or caveolin-1-deficient (Cav1−/− ... Caveolin-1 interferes with membrane protein mobility and blocks integrin-mediated uptake of S. aureus. Caveolin-1 has ... S6). To investigate the role of caveolin, we used a fibroblast line derived from caveolin-1-deficient mice (Cav1−/− cells) ( ...
CAV3: caveolin 3. *CAVIN1: caveolae associated protein 1. *CBFB: core-binding factor subunit beta ...
The CAV3 gene provides instructions for making a protein called caveolin-3, which is found in the membrane surrounding muscle ... Alias L, Gallano P, Moreno D, Pujol R, Martínez-Matos JA, Baiget M, Ferrer I, Olivé M. A novel mutation in the caveolin-3 gene ... Mutations that cause CAV3-related distal myopathy result in a shortage of caveolin-3 protein in the muscle cell membrane and a ... Mutant caveolin-3 induces persistent late sodium current and is associated with long-QT syndrome. Circulation. 2006 Nov 14;114( ...
  • Caveolin-3 is a protein that in humans is encoded by the CAV3 gene. (wikipedia.org)
  • Other mutations in Caveolin causes Long QT Syndrome or familial hypertrophic cardiomyopathy, although the role of Cav3 in Long QT syndrome has recently been disputed. (wikipedia.org)
  • Cardiac overexpression of muscle specific caveolin-3 (Cav3) protects against ischemic/reperfusion injury through Akt kinase activation. (ahajournals.org)
  • Caveolin-3 (CAV3) is a muscle-specific protein localized to the sarcolemma. (biomedcentral.com)
  • The CAV3 gene provides instructions for making a protein called caveolin-3, which is found in the membrane surrounding muscle cells. (medlineplus.gov)
  • Mutations that cause CAV3 -related distal myopathy result in a shortage of caveolin-3 protein in the muscle cell membrane and a reduction in the number of caveolae. (medlineplus.gov)
  • CAV3 gene mutations that cause isolated hyperCKemia lead to a caveolin-3 shortage that likely damages muscle cells. (medlineplus.gov)
  • CAV3 gene mutations that cause rippling muscle disease result in a shortage of caveolin-3 protein in the muscle cell membrane. (medlineplus.gov)
  • I Ca and Ca 2+ transients were measured in isolated myocytes, and expression of caveolin 3 (Cav3), junctophilin 2 (Jph2) and bridging integrator 1 (Bin1) was determined. (bris.ac.uk)
  • Skeletal muscle expressing Pro104Leu mutant caveolin 3 (CAV3 P104L ) in mouse becomes atrophied and serves as a model of autosomal dominant limb-girdle muscular dystrophy 1C. (fujita-hu.ac.jp)
  • Mutation of the caveolin-3 gene ( CAV3 ) causes this disorder. (medscape.com)
  • Caveolin-3 is one of three isoforms of the protein caveolin. (wikipedia.org)
  • Recombinant Human Caveolin-3 protein ( ab114264 ) can be used as a positive control in WB. (abcam.com)
  • The protein caveolin-3, a structural component of cardiac caveolae, is associated with cellular signaling. (ahajournals.org)
  • Ad.Cav-3 also blocked the PE- and ET-induced phosphorylations of extracellular signal-regulated kinases (ERKs) but did not affect c -Jun amino-terminal kinase and p38 mitogen-activated protein kinase activities. (ahajournals.org)
  • In the dog with pacing-induced heart failure, expression of caveolin-3 protein is increased. (ahajournals.org)
  • It has been reported that molecules involved in cardiac hypertrophic responses are concentrated in caveolae, eg, G proteins, extracellular signal-regulated kinases (ERKs), Src family kinases, Ras, and protein kinase C. 1,4 We have reported that caveolin-3 is upregulated in hypertrophied cardiomyocytes induced by phenylephrine (PE). (ahajournals.org)
  • Caveolin-3 is the major structural protein of caveolae in muscle. (nature.com)
  • Recombinant Human Caveolin-3 full length protein expressed in E.coli, shows a 50 kDa band on SDS-PAGE (Including GST). (creativebiomart.net)
  • AdipoR1/Cav-3 interaction was required for APN-initiated AMP-activated protein kinase (AMPK)-dependent and AMPK-independent intracellular cardioprotective signalings. (ahajournals.org)
  • More importantly, APPL1 and adenylate cyclase, 2 immediately downstream molecules required for AMPK-dependent and AMPK-independent signaling, respectively, formed a protein complex with AdipoR1 in a Cav-3 dependent fashion. (ahajournals.org)
  • This mutation causes a 90-95% loss of caveolin-3 protein levels and reduced formation of caveolae in skeletal muscle fibers. (biomedsearch.com)
  • In this study, we investigated the effects of palmitate on caveolin proteins and on endothelial Nitric Oxide Synthase (eNOS), a signaling mediator that binds to caveolin-3, the musclespecific caveolae scaffolding protein. (thescipub.com)
  • To determine the mechanism of the loss of plasma membrane bound eNOS, we investigated the effect of palmitate on caveolin-3 and found decreased caveolin-3 protein levels by 70% compared to control cells. (thescipub.com)
  • The interaction of caveolin 3 protein with the potassium inward rectifier channel Kir2.1: physiology and pathology related to long qt syndrome 9 (LQT9). (semanticscholar.org)
  • Caveolin-3 is a muscle specific scaffolding protein with both structural and signaling roles. (pitt.edu)
  • Caveolae require the caveolin protein for formation. (ebi.ac.uk)
  • Caveolin 3 is a membrane protein that binds cholesterol and a number of signaling molecules that interact with integrin β1. (bioscientifica.com)
  • Furthermore, caveolin 3 drove activation of the mitogen-activated protein kinase (MAPK) signaling cascades, which resulted in upregulation of cyclin D1 in FS cells. (bioscientifica.com)
  • The purpose of this study was to investigate whether MURC/cavin-4, a plasma membrane and Z-line associated protein exhibiting an overlapping distribution with Caveolin-3 (Cav-3) in heart and muscle tissues, may be expressed and play a role in rhabdomyosarcoma (RMS), an aggressive myogenic tumor affecting childhood. (elsevier.com)
  • Finally, MURC/cavin-4 silencing prevented the differentiation in the RD cell line, leading to morphological cell impairment characterized by depletion of myogenin, Cav-3 and MHC protein levels. (elsevier.com)
  • Within the caveolae, the caveolin-3 protein acts as a scaffold to organize other molecules that are important for cell signaling and maintenance of the cell structure. (medlineplus.gov)
  • Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. (novusbio.com)
  • In Western blot a band is observed ~23 kDa, representing the Caveolin 1 protein. (novusbio.com)
  • Caveolin, a 21-24-kDa integral membrane protein, is a principal component of the caveolae membrane. (elsevier.com)
  • Here, we examine (i) the expression of caveolin-3 protein in muscle tissue types and (ii) its localization within skeletal muscle fibers by immunofluorescence microscopy and subcellular fractionation. (elsevier.com)
  • A survey of tissues and muscle cell types by Western blot analysis reveals that the caveolin-3 protein is selectively expressed only in heart and skeletal muscle tissues, cardiac myocytes, and smooth muscle cells. (elsevier.com)
  • Immunolocalization of caveolin-3 in skeletal muscle fibers demonstrates that caveolin-3 is localized to the sarcolemma (muscle cell plasma membrane) and coincides with the distribution of another muscle-specific plasma membrane marker protein, dystrophin. (elsevier.com)
  • In addition, caveolin-3 protein expression is dramatically induced during the differentiation of C2C12 skeletal myoblasts in culture. (elsevier.com)
  • Our results indicare that caveolin-3 co-localizes, co-fractionates, and coimmunoprecipitates with a fusion protein containing the cytoplasmic tail of β-dystroglycan. (elsevier.com)
  • This process is the classical signaling pathway of testosterone, which requires several hours to respond [ 3 ] since it relates to gene transcriptional activation or repression, as well as protein synthesis and secretion required to support spermatogenesis [ 4 ]. (hindawi.com)
  • Caveolin-3 (Cav-3) is a protein that has been implicated in t-tubule formation and function in cardiac ventricular myocytes. (bristol.ac.uk)
  • In cell lines expressing the same mutant of caveolin-1 , we observed that the mutant Caveolin-1 expression seemed to induce cellular transformation and activation of mitogen-activated protein kinase-signaling pathway and to promote invasion-ability as well as altered actin networks in the cells. (aacrjournals.org)
  • Alignment of the protein sequences encoded by the human caveolin-1 , caveolin-2 , and caveolin-3 . (aacrjournals.org)
  • In addition, signaling studies show p38 mitogen-activated protein kinase (MAPK) as the upstream regulator of Sp1-mediated activation of the caveolin-1 promoter following oxidative stress. (aacrjournals.org)
  • Inhibition of p38 MAPK prevents the oxidant-induced Sp1-mediated up-regulation of caveolin-1 protein expression and development of premature senescence. (aacrjournals.org)
  • Caveolin is the structural protein component of caveolar membranes. (aacrjournals.org)
  • Caveolin acts as a scaffolding protein to concentrate and functionally regulate signaling molecules ( 1 - 7 ). (aacrjournals.org)
  • The direct interaction with caveolin-1 results in the inhibition of a number of signaling molecules, such as G-protein α subunit, Ras, nitric oxide synthase, protein kinase C, and protein kinase A ( 2 , 7 , 10 , 16 - 25 ). (aacrjournals.org)
  • Several independent lines of evidence indicate that caveolin-1 may act as an antiproliferative protein ( 28 - 32 ). (aacrjournals.org)
  • In the absence of caveolin-2 protein expression, caveolae still form and caveolin-1 maintains its localization in plasma membrane caveolae, although in certain tissues caveolin-1 is partially destabilized and shows modestly diminished protein levels. (asm.org)
  • A major advance in the study of caveolae was the discovery of the 21- to 24-kDa caveolar marker protein named caveolin (now called caveolin-1) ( 38 ). (asm.org)
  • This ≈20-kDa protein, named caveolin-2, was ≈38% identical and 58% similar to caveolin-1 ( 44 ). (asm.org)
  • Caveolin-3 is a muscle-specific protein related to the caveolae, which are the invaginations of the plasma membrane. (medscape.com)
  • In addition, LA419 treatment restored endothelial NO synthase and caveolin-3 expression levels, enhanced the interaction between endothelial NO synthase and its positive regulator the heat shock protein 90, and re-established the normal cardiac content of cGMP in stenosed rats. (ahajournals.org)
  • Caveolae are specialized microdomains of the plasmalemma rich in signaling molecules and supported by the structural protein caveolin-3 in muscle. (pnas.org)
  • Immunoprecipitation from ventricular myocytes using anti-Ca v 1.2 or anti-caveolin-3 followed by Western blot analysis showed that caveolin-3, Ca v 1.2, β 2 -AR (not β 1 -AR), G protein α s , adenylyl cyclase, protein kinase A, and protein phosphatase 2a are closely associated. (pnas.org)
  • Caveolae are small invaginations of the plasma membrane enriched in cholesterol and sphingolipids, which are defined by their principal structural protein, caveolin. (pnas.org)
  • Caveolin, an intergal membrane protein of the plasma membrane caveolae, binds to and regulates nitric oxide synthases (NOS) in several cells and tissues. (nii.ac.jp)
  • Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. (wikipedia.org)
  • Caveolin 3 has been shown to interact with a range of different proteins, including, but not limited to: DAG1, DYSF, EGFR, and RYR1. (wikipedia.org)
  • There are many proteins that associate with caveolin-3, including ion channels and exchangers. (wikipedia.org)
  • Disruption of interactions between caveolin-3 and its associated binding proteins has been shown to affect LTCC. (wikipedia.org)
  • This occurs by changing the PKA-mediated phosphorylation of caveolin-3-associated binding proteins, causing negative down-stream effects on LTCC activity. (wikipedia.org)
  • Three proteins, caveolin-1, caveolin-2 and caveolin-3 have been identified as principle components of caveolae. (creativebiomart.net)
  • Numerous studies have demonstrated that caveolin scaffolding domain binding inhibits the function of multiple caveolar proteins involved in cell growth and proliferation. (ahajournals.org)
  • Ceramide is a sphingolipid and localizes to caveolae, which are lined in the inner membrane leaflet by caveolin proteins. (thescipub.com)
  • Caveolin proteins are essential for distinct effects of membrane estrogen receptors in neurons. (semanticscholar.org)
  • however, it is not known whether caveolin-1 is associated with the beta(3)-AR or solely with downstream signaling proteins. (diva-portal.org)
  • Our studies indicate that interaction of the beta(3a)-AR with caveolin inhibits coupling to G alpha(i/o) proteins and suggest that signaling is modulated by a raft-enriched complex containing the beta(3a)-AR, caveolin-1, G alpha(s), and adenylyl cyclase. (diva-portal.org)
  • Mammals have three caveolin proteins:caveolin-1 (Cav-1, or VIP21), caveolin-2 and caveolin-3 (or M-caveolin). (ebi.ac.uk)
  • Evaluating caveolin interactions: do proteins interact with the caveolin scaffolding domain through a widespread aromatic residue-rich motif? (ebi.ac.uk)
  • Caveolin interacts with and regulates heterotrimeric G-proteins. (novusbio.com)
  • Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. (novusbio.com)
  • This is specific for caveolin alpha and beta proteins. (novusbio.com)
  • Several membrane-microdomain-associated proteins, such as flotillin-1 and flotillin-2, as well as caveolin, were recruited to sites of bacterial attachment. (biologists.org)
  • However, fluorescence recovery after photobleaching (FRAP) revealed an enhanced mobility of membrane-microdomain-associated proteins in the absence of caveolin-1. (biologists.org)
  • Using differentiated C2C12 skeletal myoblasts as a model system, we observe that caveolin-3 co-fractionates with cytoplasmic signaling molecules (G- proteins and Src-like kinases) and members of the dystrophin complex (dystrophin, α-sarcoglycan, and β-dystroglycan), but is clearly separated from the bulk of cellular proteins. (elsevier.com)
  • In an effort to discover other novel resident proteins of caveolae, Scherer and colleagues identified a second caveolin homologue through the microsequencing of purified adipocyte caveolar membranes. (asm.org)
  • Caveolin-1 or CAV1 is a scaffolding proteins within caveolar membranes. (thermofisher.com)
  • Caveolae, plasma membrane invaginations with constitutive caveolin proteins, harbour proteins involved in intracellular calcium ([Ca 2+ ] i ) regulation. (ersjournals.com)
  • Caveolae are flask-shaped plasma membrane invaginations expressing the scaffolding caveolin proteins-1 to -3 [ 1 , 2 ], as well as a variety of proteins important in signal transduction [ 3 ]. (ersjournals.com)
  • We and others have demonstrated that human airway smooth muscle (ASM) cells express caveolin-1 (Cav-1), and that ASM caveolae contain a number of proteins involved in regulation of intracellular Ca 2+ ([Ca 2+ ] i ) [ 6 - 8 ]. (ersjournals.com)
  • F-actin is accumulated promptly at the site of mem- (https://www.weblio.jp/content/%E4%B8%80% 2+ brane disruption and annexins, phospholipid-interacting proteins with Ca -binding activity, E8%88%AC%E8%B2%A1%E5%9B%A3%E6% are also recruited to the injured site and contribute to membrane repair [3]. (deepdyve.com)
  • MURC was also colocalized and associated with caveolin-3 at the plasma membrane of CMs, suggesting an inhibitory interaction of MURC with caveolin-3 for cAMP production in β-adrenergic signaling. (ahajournals.org)
  • MURCΔCC impaired membrane localization of caveolin-3, resulting in increased cAMP levels in CMs compared with MURC. (ahajournals.org)
  • Transgenic mice expressing cardiac-specific MURCΔCC exhibited progressive cardiac dysfunction and intraventricular conduction delay accompanied by impaired membrane localization of SCN5A, which was associated with caveolin-3. (ahajournals.org)
  • We demonstrate that the TFT caveolin-3 mutation in post-mitotic skeletal myotubes causes severely reduced localization of caveolin-3 to the plasma membrane and to lipid rafts, and significantly inhibits caveolar function. (biomedsearch.com)
  • We have addressed this question by examining the impact of membrane rafts and caveolin-1 on the differential signaling of mouse beta(3a)- and beta(3b)-AR isoforms that diverge at the distal C terminus. (diva-portal.org)
  • When cells expressing the beta(3a)-AR were treated with filipin III to disrupt membrane rafts or transfected with caveolin-1 siRNA, the cyclic AMP response to the beta(3)-AR agonist CL316243 became PTX-sensitive, suggesting G alpha(i/o) coupling. (diva-portal.org)
  • Caveolin forms oligomers and associates with cholesterol and sphingolipids in certain areas of the cell membrane , leading to the formation of caveolae . (wikipedia.org)
  • Enhanced membrane microdomain mobility and increased uptake of S. aureus was repressed by expression of wild-type caveolin-1, but not caveolin-1 G83S, which harbors a point mutation in the caveolin scaffolding domain. (biologists.org)
  • These results highlight a crucial role for caveolin-1 in negative regulation of membrane microdomain mobility, thereby affecting endocytosis of bacteria-engaged integrins. (biologists.org)
  • Thus, the pathological loss of t-tubular I Ca , which contributes to impaired excitation-contraction coupling and thereby cardiac function in vivo, appears to be attributable to loss of caveolin 3-dependent stimulation of t-tubular I Ca . Abstract: Previous work has shown redistribution of L-type Ca 2+ current (I Ca ) from the t-tubules to the surface membrane of rat ventricular myocytes after myocardial infarction. (bris.ac.uk)
  • We hypothesized that aquaporin 3 is involved in the regulation of keratinocyte function by a mechanism involving the interaction between aquaporin 3 and phospholipase D. Using sucrose gradient centrifugation, immunoprecipitation analysis, and confocal microscopy, we found that aquaporin 3 and phospholipase D 2 colocalized in caveolin-rich membrane microdomains. (elsevier.com)
  • Zheng, X & Bollag, WB 2003, ' Aquaporin 3 Colocates with Phospholipase D 2 in Caveolin-Rich Membrane Microdomains and Is Downregulated Upon Keratinocyte Differentiation ', Journal of Investigative Dermatology , vol. 121, no. 6, pp. 1487-1495. (elsevier.com)
  • However, it remains unknown whether Cav-3 expression is required for the plasma membrane recruitment and caveolar targeting of PFK-M. Here, we demonstrate that recombinant expression of Cav-3 dramatically affects the subcellular localization of PFK-M, by targeting PFK-M to the plasma membrane, and by translocating PFK-M to caveolae-enriched membrane domains. (elsevier.com)
  • Here, we demonstrate that caveolin-3 directly interacts with β-dystroglycan, an integral membrane component of the dystrophin complex. (elsevier.com)
  • Caveolin-3 overexpression promoted the localization of ADRB2 on the membrane of the HG-stimulated H9C2 cells, subsequently inhibiting apoptosis and promoting cell viability. (aging-us.com)
  • Knockdown of caveolin-1 by shRNA decreased the amount of AR localized to membrane fraction and prevented AR membrane trafficking after being exposed to testosterone at physiological concentration. (hindawi.com)
  • These data suggested that AR localized to membrane fraction by binding with caveolin-1 through palmitoylation of the cysteine residue. (hindawi.com)
  • We looked for mutations in the caveolin-1 gene, encoding a critical molecule for membrane signaling to cell growth, in 92 primary human breast cancers, and we report here the identification of a mutation in caveolin-1 at codon 132 (P132L) in 16% of cases. (aacrjournals.org)
  • This site is evolutionarily conserved from worms to man, providing evidence that this region of the membrane-spanning domain is critical for Caveolin function. (aacrjournals.org)
  • and grants from the Setsuro including calpain 3, which is involved in the resealing of membrane structures through their 2+ Fujii Memorial, the Osaka Foundation for Ca -dependent protease activity [1]. (deepdyve.com)
  • Dysferlin interacts with mitsugumin 53 (MG53) and caveolin 3, which are %B1%E4%BC%9A), the Osaka Medical Research also essential to repair membrane damage [5]. (deepdyve.com)
  • Caveolae are invaginations of the plasma membrane, located in ordered lipid raft domains (13) and enriched with caveolin-1, -2, or -3 (11,14). (deepdyve.com)
  • Caveolin-3, the most recently recognized member of the caveolin gene family, is muscle-specific and is found in both cardiac and skeletal muscle, as well as smooth muscle cells. (elsevier.com)
  • Caveolin-2 is a member of the caveolin gene family with no known function. (asm.org)
  • Overexpression of caveolin-3 leads to the development of cardiomyopathy, resulting in degeneration of cardiac tissue and manifesting pathologies due to the associated degeneration. (wikipedia.org)
  • To investigate the effect of adenovirus-mediated overexpression of caveolin-3 on hypertrophic responses in cardiomyocytes, we constructed an adenovirus that encoded human wild-type caveolin-3 (Ad.Cav-3), mutant caveolin-3 (Ad.Cav-3Δ), or bacterial β-galactosidase (Ad.LacZ). (ahajournals.org)
  • We investigated the effect of adenovirus-mediated overexpression of wild-type and dominant-negative caveolin-3 on hypertrophic responses to G q -dependent agonists in cardiomyocytes. (ahajournals.org)
  • Overexpression of caveolin-3 induces cardiac protection, and loss of caveolin-3 causes progressive cardiomyopathy. (ahajournals.org)
  • Knockdown of MURC suppressed ISO-induced cAMP production in CMs, whereas overexpression of MURC as well as knockdown of caveolin-3 stimulated cAMP production. (ahajournals.org)
  • Under HG stimulation, Caveolin-3 overexpression enhanced the activity of the cAMP/PKA and BDNF/TrkB signaling pathways, whereas ADRB2 silencing reversed the effects of Caveolin-3 overexpression. (aging-us.com)
  • Combination of electrophoretic mobility shift studies, chromatin immunoprecipitation analysis, Sp1 overexpression experiments, as well as promoter mutagenesis identifies enhanced Sp1 binding to two GC-boxes at −238/−231 and −118/−106 as the core mechanism of oxidative stress-triggered caveolin-1 transactivation. (aacrjournals.org)
  • Consistent with this idea, we have previously shown that overexpression of caveolin-1 is sufficient to arrest mouse embryonic fibroblasts in the G 0 -G 1 phase of the cell cycle, reduce their proliferative life span, and promote premature cellular senescence through activation of a p53/p21-dependent pathway ( 33 , 34 ). (aacrjournals.org)
  • Small interference RNA (siRNA) or overexpression vectors were used to alter expression of caveolin-1 (Cav-1), Orai1 or STIM1. (ersjournals.com)
  • Cardiac-Specific Caveolin-3 Overexpression Prevents Post-Myocardial Infarction Ventricular Arrhythmias by Inhibiting Ryanodine Receptor-2 Hyperphosphorylation. (physiciansweekly.com)
  • Loss of caveolin-3 induced by the dystrophy-associated P104L mutation impairs L-type calcium channel function in mouse skeletal muscle cells. (semanticscholar.org)
  • A deficiency in caveolin-3 (Cav-3), the major striated muscle isoform, is responsible for skeletal muscle disorders, such as limb-girdle muscular dystrophy 1C (LGMD 1C). (semanticscholar.org)
  • Skeletal Muscle 3 (1): 3. (els.net)
  • We previously generated a model of limb-girdle muscular dystrophy by overexpressing the disease-causing mutant caveolin-3 (CAV-3P104L) in skeletal muscle. (nii.ac.jp)
  • The results of this study demonstrate that caveolin-3 mutations associated with LGMD-1C disrupt normal cellular signal transduction pathways associated with caveolae and cause apoptosis in muscle cells, all of which may reflect pathogenetic pathways that lead to muscle degeneration in these disorders. (biomedsearch.com)
  • Whereas dominant-negative versions of flotillin-2 did not affect bacterial attachment or internalization, cells deficient for caveolin-1 ( Cav1 −/− ) showed increased uptake of S. aureus and other Fn-binding pathogens. (biologists.org)
  • Mutations affecting the CAV1 gene can result in congenital generalized lipodystrophy 3, pulmonary hypertension primary 3, or partial lipodystrophy/congenital cataracts, and neurodegeneration syndrome. (thermofisher.com)
  • Caveolin-1 and -2 are coexpressed and abundantly expressed in adipocytes, endothelial cells, and fibroblasts. (ahajournals.org)
  • Caveolin-1 is most prominently expressed in endothelial , fibrous , and adipose tissue. (wikipedia.org)
  • Caveolin-1 and caveolin-2 are coexpressed in many cell types, including adipocytes, endothelial cells, epithelial cells, and fibroblasts ( 9 ). (aacrjournals.org)
  • Although caveolin-2 is coexpressed and heterooligomerizes with caveolin-1 in many cell types (most notably adipocytes and endothelial cells), caveolin-2 has traditionally been considered the dispensable structural partner of the widely studied caveolin-1. (asm.org)
  • Santibanez JF, Blanco FJ, Garrido-Martin EM, Sanz-Rodriguez F, del Pozo MA and Bernabeu C: Caveolin-1 interacts and cooperates with the transforming growth factor-beta type I receptor ALK1 in endothelial caveolae. (spandidos-publications.com)
  • abstract = "Aquaporin 3 is a channel that transports both water and glycerol. (elsevier.com)
  • Flow Cytometry: Caveolin-1 Antibody (7C8) [Allophycocyanin] [NB100-615APC] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615APC (blue) and a matched isotype control (orange). (novusbio.com)
  • This Caveolin 1 antibody is useful for Flow Cytometry, Western blot, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Immunohistochemistry-paraffin embedded sections. (novusbio.com)
  • For this purpose, we generated a novel monoclonal antibody (mAb) probe that recognizes the unique N-terminal region of caveolin-3, but not other members of the caveolin gene family. (elsevier.com)
  • The following product was used in this experiment: Caveolin 1 Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-17447, RRID AB_10985023. (thermofisher.com)
  • This antibody is not cross-reactive with caveolin-2 or -3. (thermofisher.com)
  • Caveolin 2 antibody LS-C498386 is an unconjugated rabbit polyclonal antibody to human Caveolin 2 (CAV2). (lsbio.com)
  • 1] "Molecular cloning and developmental expression of the caveolin gene family in the amphibian Xenopus laevis. (tcdb.org)
  • The caveolin gene family consists of three members: caveolin-1, caveolin-2, and caveolin-3 ( 3 , 4 , 8 ). (aacrjournals.org)
  • A caveolin-3 mutant that causes limb girdle muscular dystrophy type 1C disrupts Src localization and activity and induces apoptosis in skeletal myotubes. (biomedsearch.com)
  • Several mutations in the muscle-specific caveolin, caveolin-3, lead to a form of autosomal dominant muscular dystrophy referred to as limb girdle muscular dystrophy type 1C (LGMD-1C). (biomedsearch.com)
  • Lack of caveolin-3 expression has been implicated in limb-girdle muscular dystrophy, along with distal myopathy and rippling muscle disease. (pitt.edu)
  • Caveolin-3 has been implicated in the development of certain types of muscular dystrophy ( Limb-girdle muscular dystrophy ). (wikipedia.org)
  • As such, our current results may be important in understanding the pathogenesis of Cav-3-related muscle diseases, such as limb-girdle muscular dystrophy-1C, distal myopathy, and rippling muscle disease, that are caused by mutations within the human Cav-3 gene. (elsevier.com)
  • Skulder-hofte-muskeldystrofi (limb girdle muscular dystrophy) er klinisk nokså homogen, men har en rekke helt ulike gen- og proteindefekter som årsak. (tidsskriftet.no)
  • Mutations in all sarcoglycans, in dysferlin, and in caveolin-3, as well as mutations that cause abnormal glycosylation of alpha-dystroglycan, can result in limb-girdle muscular dystrophy. (medscape.com)
  • Limb-girdle muscular dystrophy type 2A can result from accelerated autoproteolytic inactivation of calpain 3. (medscape.com)
  • Mutations in the proteolytic enzyme calpain 3 cause limb-girdle muscular dystrophy type 2A.Cell. (healio.com)
  • As a PhD student, I was the first to discover that mutations in the caveolin-3 gene are the cause of a novel form of limb-girdle muscular dystrophy, named LGMD-1C. (salford.ac.uk)
  • Accordingly, mutations in the calpain 3 gene cause limb Promotion of Fundamental Medical Research (YH) girdle muscular dystrophy type 2A [2]. (deepdyve.com)
  • Genetically engineered mice that lack caveolin-1 and caveolin-2 are viable and fertile, showing that neither the caveolins nor the caveolae are essential in embryonic development or reproduction of these animals. (wikipedia.org)
  • Caveolin-3 is concentrated in the caveolae of myocytes, and modulates numerous metabolic processes including: nitric oxide synthesis, cholesterol metabolism, and cardiac myocytes contraction. (wikipedia.org)
  • In cardiac myocytes, caveolin-3 negatively regulates ATP-dependent potassium channels (KATP) localized in caveolae. (wikipedia.org)
  • Caveolin-3 associates with the cardiac sodium-calcium exchanger (NCX) in caveolae of cardiac myocytes. (wikipedia.org)
  • Interactions between caveolin-3 and cardiac NCX influence NCX-regulation of cellular signaling factors and excitation of cardiac myocytes. (wikipedia.org)
  • In contrast, caveolin-3 expression is essentially restricted to skeletal and smooth muscle cells, as well as cardiac myocytes ( 10 - 18 ). (aacrjournals.org)
  • For example, β 2 -adrenergic receptor (AR) associates with Cav-3 in neonatal mouse cardiac myocytes ( 10 - 12 ). (pnas.org)
  • The endogenous beta(3a)-AR displayed G alpha(i/o) coupling in brown adipocytes from caveolin-1 knock-out mice or in wild type adipocytes treated with filipin III. (diva-portal.org)
  • These results indicate a role for caveolin-3 in efficient nAChR clustering.Electromyography studies in anesthetized mice indicated lengthened latencies of the muscle action potential in the caveolin-3 null mice as compared to wildtype mice. (pitt.edu)
  • There were also decreased overall electromyography (EMG) amplitude and EMG area under the curve in caveolin-3 null mice. (pitt.edu)
  • Aquaporin 3-deficient mice exhibit skin defects, including decreased glycerol content and impairment of water holding capacity, barrier recovery, and wound healing. (elsevier.com)
  • We therefore investigated the structure and function of myocytes isolated from the hearts of Cav-3 KO mice. (bristol.ac.uk)
  • The mutations in these mice were located at the 3' and 5' ends of the dysferlin gene. (nih.gov)
  • Vital staining with Evans blue dye revealed loss of sarcolemmal integrity in both lines of mice, similar to that seen in mdx and caveolin-3 deficient mice. (nih.gov)
  • However, in contrast to the latter group of animals, the dysferlin-deficient mice have an intact dystrophin glycoprotein complex and normal levels of caveolin-3. (nih.gov)
  • We now directly address the functional significance of caveolin-2 by genetically targeting the caveolin-2 locus (Cav-2) in mice. (asm.org)
  • As caveolin-2 expression is also severely reduced in Cav-1-null mice, we conclude that caveolin-2 deficiency is the clear culprit in this lung disorder. (asm.org)
  • Our analysis of several different phenotypes observed in caveolin-1-deficient mice (i.e., abnormal vascular responses and altered lipid homeostasis) reveals that Cav-2-null mice do not show any of these other phenotypes, indicating a selective role for caveolin-2 in lung function. (asm.org)
  • The double-mutant mice exhibited a reduction in the muscle mass and strength in comparison with the mutant caveolin-3 mice. (nii.ac.jp)
  • RESEARCH DESIGN AND METHODS Wild-type and Nur77 null mice were fed a high-fat diet (60% calories from fat) for 3 months. (diabetesjournals.org)
  • Functional interaction of the two-pore domain potassium channel TASK-1 and caveolin-3. (semanticscholar.org)
  • The beta(3a)-AR C terminus, S (P-384) under bar PLNR (P-389) under bar DG (Y-392) under bar EGARP (P-398) under bar PT, resembles a caveolin interaction motif. (diva-portal.org)
  • As the WW domain of dystrophin recognizes the same site within β-dystroglycan, we also demonstrate that caveolin-3 can effectively block the interaction of dystrophin with β-dystroglycan. (elsevier.com)
  • In this regard, interaction of caveolin-3 with β-dystroglycan may competitively regulate the recruitment of dystrophin to the sarcolemma. (elsevier.com)
  • Fluorescence lifetime imaging showed a direct interaction of dysferlin with Trim72/MG53, AHNAK, cytoplasmic dynein, myomesin-2 and calsequestrin-1, but not with caveolin-3 or dystrophin. (nih.gov)
  • Changes in the distribution and function of Cavin4/Murc, which is predominantly expressed in striated muscles, have been reported to alter caveolae structure through interaction with Caveolin-3. (uni-koeln.de)
  • However, interaction of caveolin-3 and neuronal NOS (nNOS) in myofiber has remained unknown. (nii.ac.jp)
  • The functional role of caveolin-1 is now a primary focus of the caveolar research field. (asm.org)
  • In cardiomyocytes, caveolin-3 is the dominant isotype. (ahajournals.org)
  • In cardiomyocytes, caveolin-3 might be involved in the regulation of channel functions. (ahajournals.org)
  • 8 Moreover, a recent report has suggested that caveolin-3 plays a role in the increase in sodium current amplitude in cardiomyocytes. (ahajournals.org)
  • Therefore, the aims of the present study were to (1) determine the role of caveolin-3 (Cav-3) (the predominant form of caveolin expressed in cardiomyocytes) in the cardioprotective actions of APN, and (2) investigate the molecular mechanisms responsible for Cav-3 regulation of APN transmembrane signaling. (ahajournals.org)
  • Caveolae formation and function are also regulated by caveolin-3 in cardiomyocytes (CMs). (ahajournals.org)
  • Overall, we show for the first time that a high palmitate diet leads to loss of caveolin-3 in cardiomyocytes and to coronary dysfunction of the mouse heart, via uncoupling of eNOS. (thescipub.com)
  • The combination of immunoprecipitations and blue native electrophoresis allowed us to identify previously reported partners of dysferlin - such as caveolin-3, AHNAK, annexins, or Trim72/MG53 - and new interacting partners. (nih.gov)
  • Similarly, dominant-negative genotypes for caveolin-3 increase cardiac hypertrophy, whereas increased expression of caveolin-3 inhibits the ability of the heart to hypertrophy, implicating caveolin-3 as a negative regulator of cardiac hypertrophy. (wikipedia.org)
  • These results suggest that caveolin-3 behaves as a negative regulator of hypertrophic responses, probably through suppression of ERK1/2 activity. (ahajournals.org)
  • MURC functions as a negative regulator of caveolin-3 at caveolae and modulates localization of caveolin-3 in CMs. (ahajournals.org)
  • Haines P, Samuel GH, Cohen H, Trojanowska M and Bujor AM: Caveolin-1 is a negative regulator of MMP-1 gene expression in human dermal fibroblasts via inhibition of Erk1/2/Ets1 signaling pathway. (spandidos-publications.com)
  • MURC regulates cardiac function through modulation of caveolin-3 function and localization in the cAMP signaling pathway. (ahajournals.org)
  • To understand the mechanistic importance of Murc loss of function, we assessed Caveolin-1 and 3 localization and found it to be abnormal. (uni-koeln.de)
  • However, the cellular mechanism by which loss of caveolin 3 leads to muscle atrophy is unknown. (fujita-hu.ac.jp)
  • Loss of caveolin-3 resulted in atrophic myopathy with increased sarcolemmal nNOS activity, indicating caveolin-3 binding and suppression on nNOS in myofiber. (nii.ac.jp)
  • Thus, increased sarcolemmal nNOS activity by the loss of caveolin-3 could prevent muscle atrophy in the pathogenesis leading to LGMD1C. (nii.ac.jp)
  • These defects are fully reversed by reassembling functional caveolae through expression of caveolin-3. (nature.com)
  • The expression of caveolin-3 is restricted to striated and smooth muscle . (wikipedia.org)
  • The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types. (novusbio.com)
  • Focal adhesion kinase signaling regulates the expression of caveolin 3 and beta1 integrin, genes essential for normal myoblast fusion / N. L. Quach;S. Biressi;L. F. Reichardt;C. Keller;T. A. Rando. (unitn.it)
  • Objective To evaluate the effects of N-acetycysteine on the expression of caveolin-3 (Cav-3) during myocardial ischemia - reperfusion (I/R) in diabetic rats . (bvsalud.org)
  • The amount of KATP activation during times of biological stress influences the amount of cellular damage that will occur, thus regulation of caveolin-3 expression during these times influences the amount of cellular damage. (wikipedia.org)
  • Alterations in caveolin-3 expression have been implicated in the altered expression and regulation of numerous signaling molecules involved in cardiomyopathies. (wikipedia.org)
  • Increasing severity of response to transverse aortic constriction, from hypertrophy to failure, was accompanied by graded loss of t-tubular I Ca and loss of regulation of I Ca by caveolin 3. (bris.ac.uk)
  • Phosphatidylglycerol, as a bioactive lipid, could potentially mediate the effects of the aquaporin 3-phospholipase D 2 signaling module, with aquaporin 3 as a modulatory unit, in the regulation of keratinocyte function. (elsevier.com)
  • These data suggest that FAK regulation of profusion genes, including caveolin 3 and the beta1D integrin subunit, is essential for morphological muscle differentiation. (unitn.it)
  • In hearts with MI, BDNF/TrkB relieved myocardial ischemic injury and suppressed myocardial cell apoptosis via the regulation of transient receptor potential canonical (TRPC) 3/6 channels, thus suggesting a new underlying therapy for MI [ 6 ]. (aging-us.com)
  • We have previously shown that up-regulation of caveolin-1 was required for oxidative stress-induced premature senescence in fibroblasts. (aacrjournals.org)
  • However, the molecular mechanisms underlying caveolin-1 up-regulation in senescent cells remain unknown. (aacrjournals.org)
  • Finally, we show that oxidative stress induces p38-mediated up-regulation of caveolin-1 and premature senescence in normal human mammary epithelial cells but not in MCF-7 breast cancer cells, which do not express caveolin-1 and undergo apoptosis. (aacrjournals.org)
  • Here we demonstrate that a subpopulation of L-type Ca 2+ channels is localized to caveolae in ventricular myocytes as part of a macromolecular signaling complex necessary for β 2 -adrenergic receptor (AR) regulation of I Ca,L . Immunofluorescence studies of isolated ventricular myocytes using confocal microscopy detected extensive colocalization of caveolin-3 and the major pore-forming subunit of the L-type Ca channel (Ca v 1.2). (pnas.org)
  • The molecular mechanisms underlying caveolin-associated diseases are still poorly understood. (nature.com)
  • Molecular and cellular experiments revealed that APN receptor 1 (AdipoR1) colocalized with Cav-3, forming AdipoR1/Cav-3 complex via specific Cav-3 scaffolding domain binding motifs. (ahajournals.org)
  • While the molecular mechanisms involved in nAChR clustering remain to be fully defined, we hypothesize caveolin-3 is important for nAChR clustering and overall neuromuscular junction function.Caveolin-3 and the nAChR co-localize and associate evidenced by immunofluorescence and immunoprecipitation. (pitt.edu)
  • Molecular cloning had identified three distinct caveolin genes (1 , 2 , 3 , 4) caveolin-1 , caveolin-2 , and caveolin-3 . (aacrjournals.org)
  • This study delineates for the first time the molecular mechanisms that modulate caveolin-1 gene transcription upon oxidative stress and brings new insights into the redox control of cellular senescence in both normal and cancer cells. (aacrjournals.org)
  • Caveolin-3 co-immunoprecipitates with antibodies directed against dystrophin, suggesting that they are physically associated as a discrete complex. (elsevier.com)
  • 11 However, the role of caveolin-3 in cardiomyocyte hypertrophy still remains unknown: it might promote hypertrophy, might inhibit hypertrophy, or might simply be a consequence of hypertrophy. (ahajournals.org)
  • 9 , 10 However, the role of caveolin in APN transmembrane signaling, ie, functioning as either an inhibitor or activator, has never been previously investigated. (ahajournals.org)
  • Ma X, Liu L, Nie W, Li Y, Zhang B, Zhang J and Zhou R: Prognostic role of caveolin in breast cancer: A meta-analysis. (spandidos-publications.com)
  • During the early stages of human tuberculosis, MTB induces an immune response [ 2 ] and subsequently leads to the development of lung granulomas consisting of macrophages, T cells, B cells, and fibroblasts [ 3 ]. (hindawi.com)
  • Here, we show that subcytotoxic oxidative stress generated by hydrogen peroxide application promotes premature senescence and stimulates the activity of a (−1,296) caveolin-1 promoter reporter gene construct in fibroblasts. (aacrjournals.org)
  • This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. (wikipedia.org)
  • It was reported that palmitoylation of a cysteine in the conserved region of estrogen receptor (ER) increases the physical association of ER with caveolin-1 [ 10 ], which is the main component of the caveolae in the plasma membranes found in most cell types. (hindawi.com)
  • Several independent lines of evidence indicate that caveolin-3 is localized to the sarcolemma, where it associates with the dystrophin-glycoprotein complex. (elsevier.com)
  • However, it remains unknown which component of the dystrophin complex interacts with caveolin-3. (elsevier.com)
  • Caveolin-3 directly interacts with the C-terminal tail of β-dystroglycan. (elsevier.com)
  • When bound by androgens, cytoplasmic AR translocates to the nucleus and binds to androgen response elements within androgen-responsive genes [ 3 ]. (hindawi.com)
  • Knockout of caveolin-3 genes are sufficient to induce these manifestations. (wikipedia.org)
  • Four isotypes encoded in 3 different genes have been identified (caveolin-1α and -1β, caveolin-2, and caveolin-3). (ahajournals.org)
  • Parental NIH3T3 cells were transfected, with the mutant or wild-type caveolin-1 genes introduced into pcDNA3 expression vector using a lipofection protocol. (aacrjournals.org)
  • Intriguingly, the normal increases in the transcript of caveolin 3 as well as an integrin subunit, the beta1D isoform, were suppressed by FAK inhibition. (unitn.it)
  • Previous co-immunoprecipitation studies have shown that endogenous PFK-M (phosphofructokinase, muscle-specific isoform) associates with caveolin (Cav)-3 under certain metabolic conditions. (elsevier.com)
  • Caveolin-3 (Cav-3) is the specific and predominant isoform that is expressed in muscle ( 5 ). (pnas.org)
  • Mutant beta(3a)-ARs (F389A/Y392A/F398A or P384S/F389A) promoted PTX-sensitive cAMP responses, and in situ proximity assays demonstrated an association between caveolin-1 and the wild type beta(3a)-AR but not the mutant receptors. (diva-portal.org)
  • MURC/cavin-4 Is Co-Expressed with Caveolin-3 in Rhabdomyosarcoma Tumors and Its Silencing Prevents Myogenic Differentiation in the Human Embryonal RD Cell Line. (nih.gov)
  • One of these mutations (here termed the 'TFT mutation') results in a deletion of a tripeptide (DeltaTFT(63-65)) that affects the scaffolding and oligomerization domains of caveolin-3. (biomedsearch.com)
  • The TFT mutation reduced the binding of Src to caveolin-3, diminished targeting of Src to lipid rafts, and caused abnormal perinuclear accumulation of Src. (biomedsearch.com)
  • These results provide, for the first time, genetic evidence that a functioning Caveolin-1 mutation may have a role in the malignant progression of human breast cancer. (aacrjournals.org)
  • MURC/cavin-4, especially in combination with Cav-3, may play a consistent role in the differentiation process of rhabdomyosarcoma. (nih.gov)
  • We found MURC/cavin-4 to be expressed, often concurrently with Cav-3, in mouse and human RMS, as demonstrated through in silico analysis of gene datasets and immunohistochemical analysis of tumor samples. (elsevier.com)
  • In vitro expression studies carried out using human cell lines and primary mouse tumor cultures showed that expression levels of both MURC/cavin-4 and Cav-3, while being low or undetectable during cell proliferation, became robustly increased during myogenic differentiation, as detected via semi-quantitative RT-PCR and immunoblotting analysis. (elsevier.com)
  • Furthermore, confocal microscopy analysis performed on human RD and RH30 cell lines confirmed that MURC/cavin-4 mostly marks differentiated cell elements, colocalizing at the cell surface with Cav-3 and labeling myosin heavy chain (MHC) expressing cells. (elsevier.com)
  • In noninfected cells, phenylephrine (PE) and endothelin-1 (ET) increased cell size and [ 3 H]leucine incorporation, along with the induction of sarcomeric reorganization and the reexpression of β-myosin heavy chain, indicating myocyte hypertrophy. (ahajournals.org)
  • Immunoprecipitation of MuSK shows that caveolin-3 and MuSK association peaks at 1 hour of agrin treatment in wildtype cells. (pitt.edu)
  • Agrin induced MuSK phosphorylation was decreased more significantly than the overall decrease in MuSK expression in the caveolin-3 null cells as compared to the wildtype results. (pitt.edu)
  • Then, we analyzed downstream events of the integrin β1 signaling pathway in the proliferation of FS cells and identified caveolin 3 as a potential candidate molecule. (bioscientifica.com)
  • Using specific small interfering RNA of caveolin 3, the proliferation of FS cells was inhibited. (bioscientifica.com)
  • These findings suggest that matricrine signaling in the proliferation of FS cells was transduced by a caveolin 3-mediated integrin β1 signaling pathway and subsequent activation of the MAPK pathway. (bioscientifica.com)
  • Researchers suggest that reduced caveolin-3 levels may impair the control of calcium levels in muscle cells, leading to abnormal muscle contractions in response to stimulation. (medlineplus.gov)
  • These data indicate that both TGF-Β-dependent reduction in satellite cells and impairment of myoblast differentiation contribute to the cellular mechanism underlying caveolin 3-deficient muscle atrophy. (fujita-hu.ac.jp)
  • Mutations resulting in loss-of-function of caveolin-3 cause cardiac myocyte hypertrophy, dilation of the heart, and depression of fractional shortening. (wikipedia.org)
  • In cardiac hypertrophy and failure, Cav-3 expression decreases, t-tubule structure is disrupted and excitation-contraction coupling (ECC) is impaired. (bristol.ac.uk)
  • These data suggest that Cav-3 KO causes cellular hypertrophy thereby decreasing t-tubular ICa density. (bristol.ac.uk)
  • Caveolin-1 has also been shown to play a role in the integrin signaling. (wikipedia.org)
  • There are dedicated phagocytotic integrins, such as integrin αMβ2 (complement receptor 3), that internalize particulate material ( Dupuy and Caron, 2008 ). (biologists.org)
  • The stimulation of I Ca,L in response to β 2 -AR activation was eliminated by disruption of caveolae with 10 mM methyl β-cyclodextrin or by small interfering RNA directed against caveolin-3, whereas β 1 -AR stimulation (norepinephrine plus prazosin) of I Ca,L was not altered. (pnas.org)
  • Taken together, our data show for the first time a specific role for caveolin-2 in mammalian physiology independent of caveolin-1. (asm.org)
  • Fernandez-Rojo MA and Ramm GA: Caveolin-1 function in liver physiology and disease. (spandidos-publications.com)
  • Although these receptors possess a putative ligand-binding domain, X-ray crystallography studies suggest that the ligand-binding pocket is occluded by bulky hydrophobic residues and is unable to accommodate ligands ( 2 , 3 ). (diabetesjournals.org)
  • It lacks 9 nucleotides in the caveolin scaffolding domain and behaves in a dominant-negative fashion. (ahajournals.org)
  • Consistent with its autosomal dominant form of genetic transmission, we demonstrate that LGMD-1C mutants of caveolin-3 behave in a dominant-negative fashion, causing the retention of wild type caveolin-3 at the level of the Golgi. (wikigenes.org)
  • Disruption of caveolin-3 disturbs the structure of cardiac caveolae and blocks atrial natriuretic peptide (ANP) expression, a cardiac-related hormone involved in many functions including maintaining cellular homeostasis. (wikipedia.org)
  • Normal caveolin-3 expression under conditions of stress increases cardiac cellular levels of ANP, maintaining cardiac homeostasis. (wikipedia.org)
  • However, there are also L-type Ca 2+ channels present outside of junctional complexes in cardiac muscle that may have significantly less impact on excitation-contraction coupling but could be essential for regulating other cellular functions ( 3 , 4 ). (pnas.org)
  • Caveolin-3 is most closely related to caveolin-1, but caveolin-3 mRNA is expressed only in muscle tissue types. (elsevier.com)
  • Five μl (1-3 μg/μl) of mRNA were reverse transcribed to generate cDNA using Superscript II and random hexamers (Life Technologies, Inc.) according to manufacturers' protocols. (aacrjournals.org)