Caveolin 2 is a binding partner of CAVEOLIN 1. It undergoes tyrosine phosphorylation by C-SRC PROTEIN PP60 and plays a regulatory role in CAVEOLAE formation.
Endocytic/exocytic CELL MEMBRANE STRUCTURES rich in glycosphingolipids, cholesterol, and lipid-anchored membrane proteins that function in ENDOCYTOSIS (potocytosis), transcytosis, and SIGNAL TRANSDUCTION. Caveolae assume various shapes from open pits to closed vesicles. Caveolar coats are composed of CAVEOLINS.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Detergent-insoluble CELL MEMBRANE components. They are enriched in SPHINGOLIPIDS and CHOLESTEROL and clustered with glycosyl-phosphatidylinositol (GPI)-anchored proteins.
A complex of polyene antibiotics obtained from Streptomyces filipinensis. Filipin III alters membrane function by interfering with membrane sterols, inhibits mitochondrial respiration, and is proposed as an antifungal agent. Filipins I, II, and IV are less important.
Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.
A partitioning within cells due to the selectively permeable membranes which enclose each of the separate parts, e.g., mitochondria, lysosomes, etc.
The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.
Cyclic GLUCANS consisting of seven (7) glucopyranose units linked by 1,4-glycosidic bonds.
The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. Each molecule of clathrin consists of three light chains (CLATHRIN LIGHT CHAINS) and three heavy chains (CLATHRIN HEAVY CHAINS) that form a structure called a triskelion. Clathrin also interacts with cytoskeletal proteins.
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Nonionic surfactant mixtures varying in the number of repeating ethoxy (oxy-1,2-ethanediyl) groups. They are used as detergents, emulsifiers, wetting agents, defoaming agents, etc. Octoxynol-9, the compound with 9 repeating ethoxy groups, is a spermatocide.
A homologous group of cyclic GLUCANS consisting of alpha-1,4 bound glucose units obtained by the action of cyclodextrin glucanotransferase on starch or similar substrates. The enzyme is produced by certain species of Bacillus. Cyclodextrins form inclusion complexes with a wide variety of substances.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Established cell cultures that have the potential to propagate indefinitely.
A CALCIUM-dependent, constitutively-expressed form of nitric oxide synthase found primarily in ENDOTHELIAL CELLS.
Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays.
Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.
Neoplasms composed of fatty tissue or connective tissue made up of fat cells in a meshwork of areolar tissue. The concept does not refer to neoplasms located in adipose tissue.
A subtype of dynamin found ubiquitously expressed in a variety of tissues.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Cells in the body that store FATS, usually in the form of TRIGLYCERIDES. WHITE ADIPOCYTES are the predominant type and found mostly in the abdominal cavity and subcutaneous tissue. BROWN ADIPOCYTES are thermogenic cells that can be found in newborns of some species and hibernating mammals.
Compounds containing carbohydrate or glycosyl groups linked to phosphatidylinositols. They anchor GPI-LINKED PROTEINS or polysaccharides to cell membranes.
Membrane-limited structures derived from the plasma membrane or various intracellular membranes which function in storage, transport or metabolism.
Short tracts of DNA sequence that are used as landmarks in GENOME mapping. In most instances, 200 to 500 base pairs of sequence define a Sequence Tagged Site (STS) that is operationally unique in the human genome (i.e., can be specifically detected by the polymerase chain reaction in the presence of all other genomic sequences). The overwhelming advantage of STSs over mapping landmarks defined in other ways is that the means of testing for the presence of a particular STS can be completely described as information in a database.
A family of high molecular weight GTP phosphohydrolases that play a direct role in vesicle transport. They associate with microtubule bundles (MICROTUBULES) and are believed to produce mechanical force via a process linked to GTP hydrolysis. This enzyme was formerly listed as EC
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
An NADPH-dependent enzyme that catalyzes the conversion of L-ARGININE and OXYGEN to produce CITRULLINE and NITRIC OXIDE.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The extension of endometrial tissue (ENDOMETRIUM) into the MYOMETRIUM. It usually occurs in women in their reproductive years and may result in a diffusely enlarged uterus with ectopic and benign endometrial glands and stroma.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Cytoplasmic vesicles formed when COATED VESICLES shed their CLATHRIN coat. Endosomes internalize macromolecules bound by receptors on the cell surface.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Centrifugation using a rotating chamber of large capacity in which to separate cell organelles by density-gradient centrifugation. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The protein constituents of muscle, the major ones being ACTINS and MYOSINS. More than a dozen accessory proteins exist including TROPONIN; TROPOMYOSIN; and DYSTROPHIN.
Amino acids containing an aromatic side chain.
A glucose transport protein found in mature MUSCLE CELLS and ADIPOCYTES. It promotes transport of glucose from the BLOOD into target TISSUES. The inactive form of the protein is localized in CYTOPLASMIC VESICLES. In response to INSULIN, it is translocated to the PLASMA MEMBRANE where it facilitates glucose uptake.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Src-family kinases that associate with T-CELL ANTIGEN RECEPTOR and phosphorylate a wide variety of intracellular signaling molecules.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.
Unsaturated derivatives of the steroid androstane containing at least one double bond at any site in any of the rings.
Transport proteins that carry specific substances in the blood or across cell membranes.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
A PROTEIN-TYROSINE KINASE family that was originally identified by homology to the Rous sarcoma virus ONCOGENE PROTEIN PP60(V-SRC). They interact with a variety of cell-surface receptors and participate in intracellular signal transduction pathways. Oncogenic forms of src-family kinases can occur through altered regulation or expression of the endogenous protein and by virally encoded src (v-src) genes.
A genus of owlet moths of the family Noctuidae. These insects are used in molecular biology studies during all stages of their life cycle.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
An enzyme that catalyzes the oxidation of cholesterol in the presence of molecular oxygen to 4-cholesten-3-one and hydrogen peroxide. The enzyme is not specific for cholesterol, but will also oxidize other 3-hydroxysteroids. EC
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A subtype of thioredoxin reductase found primarily in the CYTOSOL.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
A heterogeneous group of inherited MYOPATHIES, characterized by wasting and weakness of the SKELETAL MUSCLE. They are categorized by the sites of MUSCLE WEAKNESS; AGE OF ONSET; and INHERITANCE PATTERNS.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Proteins prepared by recombinant DNA technology.
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.
A 700-kDa cytosolic protein complex consisting of seven equimolar subunits (alpha, beta, beta', gamma, delta, epsilon and zeta). COATOMER PROTEIN and ADP-RIBOSYLATION FACTOR 1 are principle components of COAT PROTEIN COMPLEX I and are involved in vesicle transport between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.
GPI-linked membrane proteins broadly distributed among hematopoietic and non-hematopoietic cells. CD55 prevents the assembly of C3 CONVERTASE or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex.
A low affinity receptor that binds NERVE GROWTH FACTOR; BRAIN-DERIVED NEUROTROPHIC FACTOR; NEUROTROPHIN 3; and neurotrophin 4.
A large group of membrane transport proteins that shuttle MONOSACCHARIDES across CELL MEMBRANES.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles is covered with a lattice-like network of the protein CLATHRIN. Shortly after formation, however, the clathrin coat is removed and the vesicles are referred to as ENDOSOMES.
Enzymes that hydrolyze GTP to GDP. EC 3.6.1.-.
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
A specific monosialoganglioside that accumulates abnormally within the nervous system due to a deficiency of GM1-b-galactosidase, resulting in GM1 gangliosidosis.
Lipids containing at least one monosaccharide residue and either a sphingoid or a ceramide (CERAMIDES). They are subdivided into NEUTRAL GLYCOSPHINGOLIPIDS comprising monoglycosyl- and oligoglycosylsphingoids and monoglycosyl- and oligoglycosylceramides; and ACIDIC GLYCOSPHINGOLIPIDS which comprises sialosylglycosylsphingolipids (GANGLIOSIDES); SULFOGLYCOSPHINGOLIPIDS (formerly known as sulfatides), glycuronoglycosphingolipids, and phospho- and phosphonoglycosphingolipids. (From IUPAC's webpage)
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
GTP-BINDING PROTEINS that contain three non-identical subunits. They are found associated with members of the seven transmembrane domain superfamily of G-PROTEIN-COUPLED RECEPTORS. Upon activation the GTP-BINDING PROTEIN ALPHA SUBUNIT of the complex dissociates leaving a dimer of a GTP-BINDING PROTEIN BETA SUBUNIT bound to a GTP-BINDING PROTEIN GAMMA SUBUNIT.
A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.
An essential amino acid that is required for the production of HISTAMINE.
A family of heterotrimeric GTP-binding protein alpha subunits that were originally identified by their ability to inhibit ADENYLYL CYCLASES. Members of this family can couple to beta and gamma G-protein subunits that activate POTASSIUM CHANNELS. The Gi-Go part of the name is also spelled Gi/Go.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
The entering of cells by viruses following VIRUS ATTACHMENT. This is achieved by ENDOCYTOSIS, by direct MEMBRANE FUSION of the viral membrane with the CELL MEMBRANE, or by translocation of the whole virus across the cell membrane.

NO synthase II in mouse skeletal muscle is associated with caveolin 3. (1/243)

The inducible-type NO synthase (NOS II; iNOS) is constitutively expressed in slow-twitch skeletal muscle fibres of guinea-pigs [Gath, Closs, Godtel-Armbrust, Schmitt, Nakane, Wessler and Forstermann (1996) FASEB J. 10, 1614-1620]. Here we studied the expression of NOS II in skeletal muscle of wild-type and NOS II-deficient mice and investigated the molecular basis for the membrane association of this NOS in muscle. A basal expression of NOS II mRNA and protein was detected in skeletal muscle from untreated wild-type mice; expression increased when mice were treated with bacterial lipopolysaccharide (LPS). No NOS II was found in any tissue of untreated or LPS-treated NOS II-deficient mice. Immunoprecipitation experiments were performed with homogenates of gastrocnemius muscle from untreated or LPS-treated wild-type mice. A NOS II-specific antibody precipitated caveolin 3 in all homogenates investigated, the effect being most pronounced in skeletal muscle from LPS-treated animals. Conversely, an antibody against caveolin 3 co-precipitated NOS II in muscle homogenates. Similarly, a weak co-precipitation of NOS II and caveolin 3 was seen in homogenates of untreated murine C2C12 myotubes; co-precipitation was markedly enhanced in cells stimulated with LPS/interferon gamma. The association of NOS II with caveolin 3 might have implications for the regulation of contraction of, and/or glucose uptake by, slow-twitch muscle fibres.  (+info)

Neuregulin signaling in the heart. Dynamic targeting of erbB4 to caveolar microdomains in cardiac myocytes. (2/243)

Two of the neuregulins (NRG1 and NRG2) and their receptors (erbB2 and erbB4) are essential for normal cardiac development and can mediate hypertrophic growth and enhance survival of embryonic, postnatal, and adult rat ventricular myocytes. The expression of erbB4, the predominant NRG receptor in postnatal rat ventricular muscle, declines after midembryogenesis, and its expression is limited to cardiac myocytes. A full-length erbB4 rat cDNA isolated from neonatal ventricular muscle was found to be highly homologous to human erbB4 and contained a caveolin binding motif within the cytoplasmic kinase domain. Using the complementary techniques of detergent-free density-gradient ultracentrifugation of myocyte lysates and coimmunoprecipitation of erbB4 and caveolin-3, the caveolin isoform expressed in cardiac myocytes, erbB4 could be localized (using both approaches) to caveolar microdomains. Moreover, addition of a soluble NRG1, recombinant human glial growth factor 2, resulted in rapid (2-minute) translocation of erbB4 out of caveolar microdomain in cardiac myocytes. Thus, erbB4 is dynamically targeted to caveolar microdomains within cardiac myocytes. Its rapid translocation after NRG1 binding may contribute to receptor desensitization in the continuous presence of ligand.  (+info)

Molecular characterization of caveolin association with the Golgi complex: identification of a cis-Golgi targeting domain in the caveolin molecule. (3/243)

Caveolins are integral membrane proteins which are a major component of caveolae. In addition, caveolins have been proposed to cycle between intracellular compartments and the cell surface but the exact trafficking route and targeting information in the caveolin molecule have not been defined. We show that antibodies against the caveolin scaffolding domain or against the COOH terminus of caveolin-1 show a striking specificity for the Golgi pool of caveolin and do not recognize surface caveolin by immunofluorescence. To analyze the Golgi targeting of caveolin in more detail, caveolin mutants were expressed in fibroblasts. Specific mutants lacking the NH2 terminus were targeted to the cis Golgi but were not detectable in surface caveolae. Moreover, a 32-amino acid segment of the putative COOH-terminal cytoplasmic domain of caveolin-3 was targeted specifically and exclusively to the Golgi complex and could target a soluble heterologous protein, green fluorescent protein, to this compartment. Palmitoylation-deficient COOH-terminal mutants showed negligible association with the Golgi complex. This study defines unique Golgi targeting information in the caveolin molecule and identifies the cis Golgi complex as an intermediate compartment on the caveolin cycling pathway.  (+info)

Localization of the human caveolin-3 gene to the D3S18/D3S4163/D3S4539 locus (3p25), in close proximity to the human oxytocin receptor gene. Identification of the caveolin-3 gene as a candidate for deletion in 3p-syndrome. (4/243)

Caveolin-3, a muscle-specific caveolin-related protein, is the principal structural protein of caveolae membrane domains in striated muscle cell types (cardiac and skeletal). Recently, we identified an autosomal dominant form of limb girdle muscular dystrophy in humans that is due to mutations within exon 2 of the caveolin-3 gene (3p25). However, the detailed location of the human caveolin-3 gene and its position with regard to neighboring genes remains unknown. Here, we have isolated three independent BAC clones containing the human caveolin-3 gene. Using a PCR-based approach, we determined that these clones contain both exons 1 and 2 of the human caveolin-3 gene. In addition, we performed microsatellite marker analysis of these BAC clones, using a panel of 13 markers that are known to map within the 3p25 region. Our results indicate that these BAC clones contain the following three markers: D3S18, SHGC-1079 (also known as D3S4163) and D3S4539. Interestingly, D3S18 is a marker for two known human diseases, von Hippel-Lindau disease and 3p-syndrome. As D3S4163 and D3S4539 are known to map in the vicinity of the 3' end of the human oxytocin receptor gene, we determined if these caveolin-3 positive BACs also contain the oxytocin receptor gene. We show that (i) these BACs contain all four exons of the oxytocin receptor gene and (ii) that the genes encoding caveolin-3 and the oxytocin receptor are located approximately 7-10 kb apart and in the opposite orientation. As 3p-syndrome is characterized by cardiac septal defects and caveolin-3 is expressed primarily in the heart and skeletal muscle, caveolin-3 is a candidate gene that may be deleted in 3p-syndrome.  (+info)

Caveolin-3 upregulation activates beta-secretase-mediated cleavage of the amyloid precursor protein in Alzheimer's disease. (5/243)

Here, we investigate the involvement of caveolins in the pathophysiology of Alzheimer's disease (AD). We show dramatic upregulation of caveolin-3 immunoreactivity in astroglial cells surrounding senile plaques in brain tissue sections from authentic AD patients and an established transgenic mouse model of AD. In addition, we find that caveolin-3 physically interacts and biochemically colocalizes with amyloid precursor protein (APP) both in vivo and in vitro. Interestingly, recombinant overexpression of caveolin-3 in cultured cells stimulated beta-secretase-mediated processing of APP. Immunoreactivities of APP and presenilins were concomitantly increased in caveolin-3-positive astrocytes. Because the presenilins also form a physical complex with caveolin-3, caveolin-3 may provide a common platform for APP and the presenilins to associate in astrocytes. In AD, augmented expression of caveolin-3 and presenilins in reactive astrocytes may alter APP processing, leading to the overproduction of its toxic amyloid metabolites.  (+info)

Phenotypic behavior of caveolin-3 mutations that cause autosomal dominant limb girdle muscular dystrophy (LGMD-1C). Retention of LGMD-1C caveolin-3 mutants within the golgi complex. (6/243)

Caveolin-3, a muscle-specific caveolin-related protein, is the principal structural protein of caveolae membrane domains in striated muscle cell types (cardiac and skeletal). Autosomal dominant limb girdle muscular dystrophy (LGMD-1C) in humans is due to mutations within the caveolin-3 gene: (i) a 9-base pair microdeletion that removes three amino acids within the caveolin scaffolding domain (DeltaTFT) or (ii) a missense mutation within the membrane spanning domain (P --> L). The molecular mechanisms by which these two mutations cause muscular dystrophy remain unknown. Here, we investigate the phenotypic behavior of these caveolin-3 mutations using heterologous expression. Wild type caveolin-3 or caveolin-3 mutants were transiently expressed in NIH 3T3 cells. LGMD-1C mutants of caveolin-3 (DeltaTFT or P --> L) were primarily retained at the level of a perinuclear compartment that we identified as the Golgi complex in double-labeling experiments, while wild type caveolin-3 was efficiently targeted to the plasma membrane. In accordance with these observations, caveolin-3 mutants formed oligomers of a much larger size than wild type caveolin-3 and were excluded from caveolae-enriched membrane fractions as seen by sucrose density gradient centrifugation. In addition, these caveolin-3 mutants were expressed at significantly lower levels and had a dramatically shortened half-life of approximately 45-60 min. However, caveolin-3 mutants were palmitoylated to the same extent as wild type caveolin-3, indicating that targeting to the plasma membrane is not required for palmitoylation of caveolin-3. In conclusion, we show that LGMD-1C mutations lead to formation of unstable high molecular mass aggregates of caveolin-3 that are retained within the Golgi complex and are not targeted to the plasma membrane. Consistent with its autosomal dominant form of genetic transmission, we demonstrate that LGMD-1C mutants of caveolin-3 behave in a dominant-negative fashion, causing the retention of wild type caveolin-3 at the level of the Golgi. These data provide a molecular explanation for why caveolin-3 levels are down-regulated in patients with this form of limb girdle muscular dystrophy (LGMD-1C).  (+info)

The limb-girdle muscular dystrophies-multiple genes, multiple mechanisms. (7/243)

In the field of muscular dystrophy, advances in understanding the molecular basis of the various disorders in this group have been rapidly translated into readily applicable diagnostic tests, allowing the provision of more accurate prognostic and genetic counselling. The limb-girdle muscular dystrophies (LGMD) have recently undergone a major reclassification according to their genetic basis. Currently 13 different types can be recognized. Amongst this group, increasing diversity of the mechanisms involved in producing a muscular dystrophy phenotype is emerging. Recent insights into the involvement of the dystrophin glycoprotein complex in muscular dystrophy suggests that its members may play distinct or even multiple roles in the maintenance of muscle fibre integrity. In other forms of LGMD, proteins have been implicated which may be important in intracellular signalling, vesicle trafficking or the control of transcription. As these various mechanisms are more fully elucidated, further insights will be gained into the pathophysiology of muscular dystrophy. At a practical level, despite the marked heterogeneity of this group real progress can at last be made in determining a precise diagnosis.  (+info)

Codistribution of NOS and caveolin throughout peripheral vasculature and skeletal muscle of hamsters. (8/243)

In isolated cell systems, nitric oxide synthase (NOS) activity is regulated by caveolin (CAV), a resident caveolae coat protein. Because little is known of this interaction in vivo, we tested whether NOS and caveolin are distributed together in the intact organism. Using immunohistochemistry, we investigated the localization of constitutive neuronal (nNOS) and endothelial (eNOS) enzyme isoforms along with caveolin-1 (CAV-1) and caveolin-3 (CAV-3) throughout the systemic vasculature and peripheral tissues of the hamster. The carotid artery, abdominal aorta, vena cava, femoral artery and vein, feed artery and collecting vein of the cheek pouch retractor muscle, capillaries and muscle fibers of retractor and cremaster muscles, and arterioles and venules of the cheek pouch were studied. In endothelial cells, eNOS and CAV-1 were present throughout the vasculature, whereas nNOS and CAV-3 were absent except in capillaries, which reacted for nNOS. In smooth muscle cells, nNOS and CAV-1 were also expressed systemically, whereas eNOS was absent; CAV-3 was present in the arterial but not the venous vasculature. Both nNOS and CAV-3 were located at the sarcolemma of skeletal muscle fibers, which were devoid of eNOS and CAV-1. These immunolabeling patterns suggest functional interactions between eNOS and CAV-1 throughout the endothelium, regional differences in the modulation of nNOS by caveolin isoforms in vascular smooth muscle, and modulation of nNOS by CAV-3 in skeletal muscle.  (+info)

This issue contains an important contribution from the team of Douglas Lauffenburger, Chair of the Editorial Board, at MIT, USA, in which they demonstrate the ability to characterise quantitative data on phenotypic behaviour of individual endothelial cells in response to angiogenesis signalling and relate it to overall population behaviour. They show that the behaviour of microvascular endothelial cells in a single population is heterogeneous and cannot be assumed to be the same for all cells in a given condition.. Endothelial cell phenotypic behaviors cluster into dynamic state transition programs modulated by angiogenic and angiostatic ...
This article presents a high-level conceptual framework to help orient the discussion and implementation of open-endedness in evolutionary systems. Drawing upon earlier work by Banzhaf et al. (2016), three different kinds of open-endedness are identified: exploratory, expansive, and transformational. These are characterized in terms of their relationship to the search space of phenotypic behaviors. A formalism is introduced to describe three key processes required for an evolutionary process: the generation of a phenotype from a genetic description, the evaluation of that phenotype, and the reproduction with variation of individuals according to their evaluation. The formalism makes explicit various influences in each of these processes that can easily be overlooked. The distinction is made between intrinsic and extrinsic implementations of these processes. A discussion then investigates how various interactions between these processes, and their modes of implementation, can lead to ...
Title: Coordinated Expression of Pax-5 and FAK1 in Metastasis. VOLUME: 11 ISSUE: 7. Author(s):Nicolas Crapoulet, Pierre OBrien, Rodney J. Ouellette and Gilles A. Robichaud. Affiliation:Universite de Moncton, Moncton, NB, Canada, E1A 3E9.. Keywords:Cancer, cell signaling, FAK1, focal adhesion, metastasis, Pax-5, B lymphopoiesis, cell differentiation, homeostasis, leukemia. Abstract: The Pax-5 gene encodes a B-cell-specific activator protein (BSAP) that plays a key role in B lymphocyte differentiation and embryogenesis. The deregulation of this transcription factor is also linked to B cell malignancies and recently to other cancers. More specifically, the downstream effects of Pax-5 promote cell-cell interactions and mediate the activation of adhesion genes which result in an epithelial phenotypic behavior of human carcinoma cells. To gain a better understanding of Pax-5-mediated gene regulation, we studied available gene expression data in depth and identified several Pax-5 downstream targets. ...
The proteoglycan-dystrophin complex in genetic cardiomyopathies--lessons from three siblings with limb-girdle muscular dystrophy-2I (LGMD-2I).: Caveolins in Cancer Pathogenesis, Prevention and Therapy [4196990] - Caveolins play an important role in the pathogenesis of multiple cancers. This volume focuses mainly on the importance of Caveolin-1 in breast, prostate, lung, skin, colon, pancreatic and brain cancers. It also studies the role of Caveolin-3 in breast cancer.Caveolins are important structural proteins of Caveolae, small invaginations of the
To increase tolerance to abiotic stresses in breeding programmes, typically families and collections of genotypes are evaluated in series of trials (environments) representing different levels of stress. The statistical analysis of the data from such trials concentrates on modelling the phenotypic behaviour of the genotypes across the set of environments. This phenotypic behaviour can be modelled in the form of genotype-specific linear and non-linear response curves in relation to environmental characterizations. Non-parallelism of the response curves indicates genotype × environment interaction. Identification of the genetic basis of the parameters determining the response curves will help in the development of breeding programmes for improving abiotic stress tolerance and understanding genotype × environment interaction. In this paper we present two strategies for locating quantitative trait loci for response-curve parameters and estimation of their allele effects. The procedures are ...
Caveolin-3, the muscle-specific isoform of caveolins, plays important roles in signal transduction. Dominant-negative mutations of the caveolin-3 gene cause autosomal dominant limb-girdle muscular dystrophy 1C (LGMD1C) with loss of caveolin-3. However, identification of the precise molecular mechanism leading to muscular atrophy in caveolin-3-deficient muscle has remained elusive. Myostatin, a member of the muscle-specific TGF-β superfamily, negatively regulates skeletal muscle volume. Here we report that caveolin-3 inhibited myostatin signaling by suppressing activation of its type I receptor; this was followed by hypophosphorylation of an intracellular effector, Mad homolog 2 (Smad2), and decreased downstream transcriptional activity. Loss of caveolin-3 in P104L mutant caveolin-3 transgenic mice caused muscular atrophy with increase in phosphorylated Smad2 (p-Smad2) as well as p21 (also known as Cdkn1a), a myostatin target gene. Introduction of the myostatin prodomain, an inhibitor of ...
At the European Molecular Biology Laboratory (EMBL) in Heidelberg, Professor Parton was mentored by Gareth Griffiths, where he honed his skills in electron microscopy while collaborating with other groups throughout the institute including Jean Gruenberg and Marino Zerial. He has maintained these collaborations to this day, despite the groups now being spread around the globe.. Collaboration has been central to his career. In a collaborative project with the laboratory of Kai Simons, he observed that the newly-discovered protein, VIP21 (later renamed caveolin-1), was an abundant marker protein of caveolae. Also at the EMBL Parton and Michael Way discovered a second member of the caveolin family, M-caveolin, now termed caveolin-3.. At The University of Queensland he collaborated with Professor John Hancock. Together they showed that caveolin mutants can act as dominant negative inhibitory mutants and that one of the mutants was a highly potent inhibitor of Ras signalling. Inhibition was specific ...
opamine D3 receptor, Caveolin1, Caveolin 1, 3 dopamine receptor, D, Dopamine receptor D3, DRD, ETM1, FET1, Cav-1, ETM1, CAV 1, CAV1, CAV1_HUMAN.
This download caveolins was been, and received on the treatment by Sir Henry Irving in 1875. Harold was in 1876, The Cup in 1881( at the Lyceum), The Promise of May( at the Globe) in 1882, Becket in 1884, with The Foresters in 1892. The download is one from which I give, not right of secure single-player of the purchase and of of rendering for the analysis.
Biochemical network reconstructions have become popular tools in systems biology. Metabolicnetwork reconstructions are biochemically, genetically, and genomically (BiGG) structured databases of biochemical reactions and metabolites. They contain information such as exact reaction stoichiometry, reaction reversibility, and the relationships between genes, proteins, and reactions. Network reconstructions have been used extensively to study the phenotypic behavior of wild-type and mutant stains under a variety of conditions, linking genotypes with phenotypes. Such phenotypic simulations have allowed for the prediction of growth after genetic manipulations, prediction of growth phenotypes after adaptive evolution, and prediction of essential genes. Additionally, because network reconstructions are organism specific, they can be used to understand differences between organisms of species in a functional context.There are different types of reconstructions representing various types of biological networks
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This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites. [provided by RefSeq, Jul 2008 ...
Expression of caveolins in RMS tumours. Double immunostain showing that in skeletal muscle Cav-1 and Cav-3 mark satellite cells and the plasmalemma of myofibres
For phenotypic behavior to be understood in the context of cell lineage and local environment, properties of individual cells must be measured relative to population-wide traits. However, the inability to accurately identify, track and measure thousands of single cells via high-throughput microscopy has impeded dynamic studies of cell populations. We demonstrate unique labeling of cells, driven by the heterogeneous random uptake of fluorescent nanoparticles of different emission colors. By sequentially exposing a cell population to different particles, we generated a large number of unique digital codes, which corresponded to the cell-specific number of nanoparticle-loaded vesicles and were visible within a given fluorescence channel. When three colors are used, the assay can self-generate over 17,000 individual codes identifiable using a typical fluorescence microscope. The color-codes provided immediate visualization of cell identity and allowed us to track human cells with a success rate of ...
A pacing system delivers cardiac protection pacing to protect the heart from injuries associated with ischemic events. The pacing system detects an ischemic event and, in response, initiates one or more cardiac protection pacing sequences each including alternative pacing and non-pacing periods. In one embodiment, the pacing system initiates cardiac protection pacing sequences including at least one postconditioning sequence to protect the heart from a detected ischemic event and a plurality prophylactic preconditioning sequences to protect the heart from probable future ischemic events.
A pacing system delivers cardiac protection pacing to protect the heart from injuries associated with ischemic events. The pacing system detects an ischemic event and, in response, initiates one or more cardiac protection pacing sequences each including alternative pacing and non-pacing periods. In one embodiment, the pacing system initiates a cardiac protection pacing sequence in response to the detection of the onset of an ischemic event, such that a pacing concurrent conditioning therapy is applied during the detected ischemic event.
Dysferlin兔多克隆抗体(ab15108)可与狗, 人样本反应并经WB, IHC, ICC/IF实验严格验证,被3篇文献引用并得到3个独立的用户反馈。所有产品均提供质保服务,中国75%以上现货。
Caveolae are specialized flask-shaped lipid rafts enriched in cholesterol, sphingolipids, and structural marker proteins termed caveolins. Caveolins are highly conserved hairpin loop-shaped, oligomeric proteins of 22-24 kDa. Besides the plasma cell membrane, caveolins are also present in mitochondri …
The limb‐girdle muscular dystrophies (LGMDs) area group of genetically heterogeneous neuromuscular disorders caused by specific protein defects in muscle fibres and characterised by predominant weakness and wasting in proximal limb and axial muscles
TY - CHAP. T1 - Role of caveolae in the airway. AU - Pabelick, Christina M. AU - Singh, Brij B.. AU - Prakash, Y.s.. PY - 2013/11/1. Y1 - 2013/11/1. N2 - Caveolae are flask-shaped invaginations of the plasma membrane that are rich in lipids and serve as microdomains to facilitate interactions between proteins at the membrane as well as intracellular components, thus modulating signal transduction, protein and lipid transport, and other processes. Constituent caveolar proteins such as caveolins and cavins also have scaffolding domains that anchor and regulate protein function. There is now evidence that caveolae and their constituent proteins are present in airway smooth muscle in a variety of species. Caveolae in airway cells contain or interact with molecules such as receptors, ion channels, and regulatory proteins that are key to the roles of airway epithelium and smooth muscle in regulating airway structure and function. Furthermore, caveolar protein expression and regulation appear to be ...
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity).
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Caveolin-2兔单克隆抗体[EPR2220(3)](ab124883)可与人样本反应并经WB, Flow Cyt实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Plasmid CAV1-mEGFP from Dr. Ari Heleniuss lab contains the insert caveolin-1 and is published in Traffic. 2010 Mar . 11(3):361-82. This plasmid is available through Addgene.
In this study, we examined cardiomyocyte hypertrophy by several methods. PE and ET increased leucine incorporation and cell area and changed the immunostaining pattern of phalloidin from a dense staining without agonists to fiberlike staining, indicating that myocyte hypertrophy did occur at the protein and structural levels. Moreover, βMHC expression was markedly augmented by PE and ET, suggesting the transformation of myosin. These results indicate that PE and ET induced pathologic hypertrophy in cardiomyocytes. Infection with Ad.Cav-3 prevented all of these changes induced by PE and ET. Thus, caveolin-3 might act as an inhibitor of myocyte hypertrophy. The effects of overexpression of wild-type caveolin-3 were not nonspecific, because infection with Ad.LacZ did not affect myocyte hypertrophy. Overexpression of caveolin-3 in Ad.Cav-3-infected cells was associated with the prevention of both agonist-induced hypertrophy and sarcomeric disorganization seen in Ad.LacZ-infected and control cells ...
Caveolins act as scaffold proteins in multiprotein complexes and have been implicated in signaling by G protein-coupled receptors. Studies using knock-out mice suggest that beta(3)-adrenoceptor (beta(3)-AR) signaling is dependent on caveolin-1; however, it is not known whether caveolin-1 is associated with the beta(3)-AR or solely with downstream signaling proteins. We have addressed this question by examining the impact of membrane rafts and caveolin-1 on the differential signaling of mouse beta(3a)- and beta(3b)-AR isoforms that diverge at the distal C terminus. Only the beta(3b)-AR promotes pertussis toxin (PTX)-sensitive cAMP accumulation. When cells expressing the beta(3a)-AR were treated with filipin III to disrupt membrane rafts or transfected with caveolin-1 siRNA, the cyclic AMP response to the beta(3)-AR agonist CL316243 became PTX-sensitive, suggesting G alpha(i/o) coupling. The beta(3a)-AR C terminus, S (P-384) under bar PLNR (P-389) under bar DG (Y-392) under bar EGARP (P-398) under ...
Coronary Artery disease is one of the leading causes of death in china, followed by cancer. According to US CDC (Centre for Disease Control), cardiovascular disease is also the leading cause of death of men and women in the United States. Factors including hereditary and family history, nutrition and lifestyle, hypertension, high lipids and cholesterol etc, will increase the risk for cardiovascular diseases. A persons unique genetic makeup will also impact their responses to drugs eg. dosage, side effects etc. Cardiac Protection Genetic Test is conducted a CAP and CLIA accredited genetic laboratory in California US.. ...
Buy our Caveolin-2 peptide. Ab4929 is a blocking peptide and has been validated in BL. Abcam provides free protocols, tips and expert support for WB and a 12…
Caveolin-3 is a protein that in humans is encoded by the CAV3 gene. Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites. This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), HyperCKemia, distal myopathy or rippling muscle disease (RMD). Other mutations in Caveolin causes Long QT Syndrome or familial hypertrophic cardiomyopathy, although the role of Cav3 in Long QT syndrome has recently been disputed. Caveolin ...
Introduction: Caveolins (Cav), the principal structural proteins of the caveolar domain, have been implicated in the development of cardiac hypertrophy, pulmonary hypertension (PH) and lung remodeling. Mice with homozygous deletion of the Cav-1 gene display cardiac hypertrophy and pulmonary abnormalities characterized by thickened alveolar septa, hypercellularity and PH. In vivo administration of a cell-permeable Cav-1 mimetic peptide was previously shown to prevent the development of monocrotaline-induced pulmonary artery medial hypertrophy, PH and right ventricular hypertrophy in rats. Whether administration of such a Cav-1 peptide could rescue the cardio-pulmonary defects observed in Cav-1 KO mice remains unknown.. Methods and Results: Three week-old wild-type and Cav-1 KO mice were randomly assigned to receive a daily intraperitoneal injection of either saline, penetratin alone (AP, 2.5 mg/kg/d) or a peptide consisting of the Cav-1 scaffolding domain coupled to penetratin (AP-Cav, 2.5 ...
Caveolin-1 (Cav-1) is a 21?kDa protein enriched in caveolae and continues to be implicated in oncogenic cell transformation CHC tumorigenesis and metastasis. response to chemotherapy and radiation and tumor growth. We found Cav-1 is definitely overexpressed in human being Personal computer cell lines mouse models and human being pancreatic tumors and is associated with worse tumor grade and clinical results. In Personal computer cell lines disruption/depletion of caveolae/Cav-1 reduces proliferation colony formation and invasion. Radiation and chemotherapy up-regulate Cav-1 manifestation while Cav-1 depletion induces both chemosensitization and radiosensitization through modified apoptotic and DNA restoration signaling. and and transgene (KPC mouse) (Fig. 1B). Cav-1 manifestation was also tested on a cells microarray of 110 individuals with pancreatic malignancy and obtained semi-quantitatively for low versus high CHC manifestation. While Cav-1 is definitely virtually absent in pancreatic ...
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TY - JOUR. T1 - Caveolin-2 is targeted to lipid droplets, a new membrane domain in the cell. AU - Fujimoto, Toyoshi. AU - Kogo, Hiroshi. AU - Ishiguro, Kimiko. AU - Tauchi, Kumi. AU - Nomura, Ryuji. PY - 2001/3/5. Y1 - 2001/3/5. N2 - Caveolin-1 and -2 constitute a framework of caveolae in nonmuscle cells. In the present study, we showed that caveolin-2, especially its β isoform, is targeted to the surface of lipid droplets (LD) by immunofluorescence and immunoelectron microscopy, and by subcellular fractionation. Brefeldin A treatment induced further accumulation of caveolin-2 along with caveolin-1 in LD. Analysis of mouse caveolin-2 deletion mutants revealed that the central hydrophobic domain (residues 87-119) and the NH2-terminal (residues 70-86) and COOH-terminal (residues 120-150) hydrophilic domains are all necessary for the localization in LD. The NH2- and COOH-terminal domains appeared to be related to membrane binding and exit from ER, respectively, implying that caveolin-2 is ...
where to get vector overexpressing caveolin-1? - posted in Molecular Cloning: Trying to overexpress caveolin-1 in human cancer cells...are there readymade products out there which already has cav-1 attached to a dependable promoter? If so, where should I look? Thanks in advance!
Proteins and other substances can cross the endothelial layer that lines a blood vessel via two routes. Caveolin-1 is essential for both, Siddiqui et al. show.. Researchers already knew that caveolin-1 was necessary for transcellular protein trafficking, in which macromolecules such as albumin enter an endothelial cell from the bloodstream and exit on the tissue side. Caveolae swallow these molecular travelers and bundle them into vesicles that wend through the cell. In an alternative pathway, known as the paracellular route, molecules slip between the cells of the endothelial layer, passing through the adherens junctions that fasten adjacent cells together. Previous work showed that adherens junctions become permeable in mice lacking caveolin-1, suggesting that the protein helps seal the junctions.. Siddiqui et al. dissected the molecular chain of events that connects caveolin-1 to adherens junction integrity. Loss of caveolin-1 activated the enzyme eNOS, which spawns nitric oxide (NO) that ...
The scaffolding protein CAV1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to…
Limb-girdle muscular dystrophy (LGMD) is a rare heterogeneous group of human diseases. Besides the characteristic presentation of weakness in the proximal limbs and shoulder or pelvic girdles, the subtypes of LGMD do not share many features in common. The group is classified, by mode of inheritance, into LGMD1 (autosomal dominant) and LGMD2 (autosomal recessive). The forms are further sub grouped based on the causative gene loci. Spontaneous animal models of LGMD are uncommon and the majority of models are in transgenic mice. Since mutations that result in LGMD sometimes result in other phenotypes, many of these animal models are useful beyond the scope of LGMD. In general the animal models of LGMD do not closely resemble the human phenotype, with a few exceptions. Although many of these models do not exactly replicate the human disease, they are still valuable tools in biomedical research, not only to further studies in understanding the mechanisms of the disease, but also to identify and test ...
TY - JOUR. T1 - AMP-dependent kinase inhibits oxidative stress-induced caveolin-1 phosphorylation and endocytosis by suppressing the dissociation between c-Abl and Prdx1 proteins in endothelial cells. AU - Takeuchi, Kimio. AU - Morizane, Yuki. AU - Kamami-Levy, Cynthia. AU - Suzuki, Jun. AU - Kayama, Maki. AU - Cai, Wenyi. AU - Miller, Joan W.. AU - Vavvas, Demetrios G.. PY - 2013/7/12. Y1 - 2013/7/12. N2 - Caveolin-1 is the primary structural component of endothelial caveolae that is essential for transcellular trafficking of albumin and is also a critical scaffolding protein that regulates the activity of signaling molecules in caveolae. Phosphorylation of caveolin-1 plays a fundamental role in the mechanism of oxidant-induced vascular hyper permeability. However, the regulatory mechanism of caveolin-1 phosphorylation remains unclear. Here we identify a previously unexpected role for AMPKin inhibition of caveolin-1 phosphorylation under oxidative stress. A pharmacological activator of AMPK, ...
Lipids are the energy source used during liver regeneration. The research group, led by Dr. Albert Pol and with members from IDIBAPS, Universitat de Barcelona and Queensland University, unveils the essential role of the protein caveolin-1 in a fundamental process for liver cure after injury or transplant. Results also evidence the existence of cellular mechanisms by which excessive accumulation of lipids in the liver is a risk factor for the apparition of hepatic tumours.
Caveolae are specialised forms of lipid rafts in the plasma membrane of most cells. They form dynamic assemblies of sphingolipids and cholesterol containing scaffolding domains with different affinities for proteins resulting in a variety of functions [1]. The constitutive Cav-1 protein is distributed ubiquitously, while Cav-2 is usually associated with Cav-1 [3, 28]. Although Cav-3 is thought to be muscle specific and is expressed in striated muscles [3, 28, 29], we and others have found that Cav-3 is not expressed within human ASM [6, 30]. Recent studies have established that ASM caveolae contain a number of proteins important to [Ca2+]i regulation (e.g. agonist receptors, Ca2+ influx channels, and force regulatory proteins such as RhoA). In canine ASM, it has been established that caveolar-enriched membrane fractions express Cav-1, L-type Ca2+ channels and plasma membrane Ca2+ ATPase, but not SR proteins such as IP3R or RyR channels [31].. In ASM of different species, in addition to Ca2+ ...
Dysferlinopathies are a group of progressive muscular dystrophies characterized by mutations in the gene DYSF. These mutations cause scarcity or complete absence of dysferlin, a protein that is expressed in skeletal muscle and plays a role in membrane repair. Our objective was to unravel the protein …
Garg V, Sun W, Hu K (2009). "Caveolin-3 negatively regulates recombinant cardiac K(ATP) channels". Biochem. Biophys. Res. ... 2008). "Protein kinase C-epsilon induces caveolin-dependent internalization of vascular adenosine 5'-triphosphate-sensitive K+ ... 385 (3): 472-7. doi:10.1016/j.bbrc.2009.05.100. PMID 19481058. ABCC9 human gene location in the UCSC Genome Browser. ABCC9 ... 52 (3): 499-506. doi:10.1161/HYPERTENSIONAHA.108.110817. PMID 18663158. Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a ...
... has been shown to interact with Caveolin 3 in skeletal muscle, and this interaction is thought to retain dysferlin ... Hernández-Deviez DJ, Howes MT, Laval SH, Bushby K, Hancock JF, Parton RG (2008). "Caveolin regulates endocytosis of the muscle ... Dysferlin also interacts with MG53, and a functional interaction between dysferlin, caveolin-3 and MG53 is thought to be ... caveolin-3, and dysferlin". J. Biol. Chem. 284 (23): 15894-902. doi:10.1074/jbc.M109.009589. PMC 2708885. PMID 19380584. ...
Scherer PE, Lisanti MP (Aug 1997). "Association of phosphofructokinase-M with caveolin-3 in differentiated skeletal myotubes. ... 63 (3): 1154-65. doi:10.2337/db13-1301. PMC 3931395. PMID 24306210. Su Y, Zhou A, Al-Lamki RS, Karet FE (May 2003). "The a- ... 61 (3): 415-9. doi:10.1016/0009-8981(75)90434-9. PMID 125160. Zhao ZZ, Malencik DA, Anderson SR (Feb 1991). "Protein-induced ... 8 (3): 273-5. doi:10.1002/(SICI)1098-1004(1996)8:3. 3.0.CO;2-#. PMID 8889589. S2CID 196597214. ...
LQT9 is caused by variants in the membrane structural protein, caveolin-3. Caveolins form specific membrane domains called ... Similar to LQT3, these caveolin variants increase the late sustained sodium current, which impairs cellular repolarization. ... 4 (3): eaar2631. Bibcode:2018SciA....4.2631H. doi:10.1126/sciadv.aar2631. PMC 5842040. PMID 29532034. Demirbilek H, Galcheva S ...
LQT9 is caused by variants in the membrane structural protein, caveolin-3. Caveolins form specific membrane domains called ... Similar to LQT3, these caveolin variants increase the late sustained sodium current, which impairs cellular repolarization. ... 24 (1): 3-13. doi:10.1016/j.spen.2017.01.001. PMID 28779863. Syncope can lead to convulsions and can easily be confused with ... Both sets of guidelines agree that LQTS can also be diagnosed if an individual has a Schwartz score of greater than 3 or if a ...
... has been shown to interact with FYN, C-src tyrosine kinase, Src, NCK1, Grb2, Caveolin 3 and SHC1. Actin-binding ... Identification of a central WW-like domain within caveolin family members". The Journal of Biological Chemistry. 275 (48): ... "Caveolin-3 directly interacts with the C-terminal tail of beta -dystroglycan. ... 23 (3): 338-42. doi:10.1038/15519. PMID 10610181. S2CID 564897. Rentschler S, Linn H, Deininger K, Bedford MT, Espanel X, Sudol ...
IGFBP-3 is one of six IGF binding proteins (IGFBP-1 to IGFBP-6) that have highly conserved structures and bind the insulin-like ... The main IGFBP-3 ligands in the circulation are IGF-1 and IGF-2, and the acid-labile subunit (ALS). The serum proteins ... IGFBP-3 exerts antiproliferative effects in many cell types by blocking the ability of IGF-1 and IGF-2 to activate the IGF1R ( ... Since IGFBP-3 is abundant in the bloodstream of healthy adults (typically 2-4 mg/L), and is largely stabilized by its complex ...
"Interaction of synthetic peptides corresponding to the scaffolding domain of Caveolin-3 with model membranes". Biopolymers. 84 ... 23 (3): 413-418. doi:10.1016/S0196-9781(01)00628-3. PMID 11835989. S2CID 1410192. Pallavi, B.; Nagaraj, R. (2003). " ...
Zhao G, Simpson RU (2010). "Membrane Localization, Caveolin-3 Association and Rapid Actions of Vitamin D Receptor in Cardiac ... 49 (3): 668-73. PMC 1683124. PMID 1652893. Szpirer J, Szpirer C, Riviere M, Levan G, Marynen P, Cassiman JJ, Wiese R, DeLuca HF ... 60 Suppl 3: 106-10. PMID 11979895. Uitterlinden AG, Fang Y, Van Meurs JB, Pols HA, Van Leeuwen JP (2004). "Genetics and biology ... 3 (3): 361-70. doi:10.1016/S1097-2765(00)80463-3. PMID 10198638. Tagami T, Lutz WH, Kumar R, Jameson JL (December 1998). "The ...
... has been shown to interact with: ARF1, Aldolase A, Amphiphysin, BIN1, Caveolin 1, Glyceraldehyde 3-phosphate dehydrogenase ... functional interaction with caveolin in low-density membrane microdomains". FEBS Letters. 467 (2-3): 326-32. doi:10.1016/s0014- ... functional interaction with caveolin in low-density membrane microdomains". FEBS Letters. 467 (2-3): 326-32. doi:10.1016/S0014- ... N-(2-(1-(3-fluorophenyl)-4-oxo-1,3,8-triazaspiro[4.5]decan-8-yl)ethyl)-2-naphthamide: 75-fold selective versus PLD1, IC50 = 20 ...
2005). "Junctophilin type 2 is associated with caveolin-3 and is down-regulated in the hypertrophic and dilated ... 325 (3): 852-6. doi:10.1016/j.bbrc.2004.10.107. PMID 15541368. Kim J, Bhinge AA, Morgan XC, Iyer VR (2005). "Mapping DNA- ... 273 (3): 920-7. doi:10.1006/bbrc.2000.3011. PMID 10891348. Garbino A, van Oort RJ, et al. (2009). "Molecular evolution of the ... 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. Matsushita Y, Furukawa T, Kasanuki H, et al. ( ...
G-protein-coupled receptor signaling components localize in both sarcolemmal and intracellular caveolin-3-associated ... 5(3):237-48. García-Marcos, M., Ghosh, P., and M.G. Farquhar. 2009. GIV is a non-receptor GEF factor for Galphai with a unique ... Retrieved 3 May 2019. Shearer, B. F.; Shearer, B. S., eds. (1996). Notable women in the life sciences : a biographical ... 2006 Jul 3; PMID 16818493 Farquhar, M. G. 2006. The glomerular basement membrane: not gone, just forgotten. J. Clin. Invest. ...
Caveolin proteins like caveolin-1 (CAV1), caveolin-2 (CAV2), and caveolin-3 (CAV3), play significant roles in the caveolar ... When caveolin proteins bind to the inner leaflet via cholesterol, the membrane starts to bend, leading to spontaneous curvature ... This effect is due to the force distribution generated when the caveolin oligomer binds to the membrane. The force distribution ... They consist of the cholesterol-binding protein caveolin (Vip21) with a bilayer enriched in cholesterol and glycolipids. ...
Llorente A, de Marco MC, Alonso MA (Oct 2004). "Caveolin-1 and MAL are located on prostasomes secreted by the prostate cancer ... Millán J, Puertollano R, Fan L, Alonso MA (Apr 1997). "Caveolin and MAL, two protein components of internal detergent-insoluble ... 233 (3): 707-12. doi:10.1006/bbrc.1997.6530. PMID 9168919. Martín-Belmonte F, Kremer L, Albar JP, Marazuela M, Alonso MA (Apr ... 82 (3): 550-62. doi:10.1046/j.1471-4159.2002.00987.x. PMID 12153479. S2CID 39619359. Copie-Bergman C, Plonquet A, Alonso MA, ...
Llorente A, de Marco MC, Alonso MA (2005). "Caveolin-1 and MAL are located on prostasomes secreted by the prostate cancer PC-3 ... 19 (3): 925-33. PMID 15168355. Rohan S, Tu JJ, Kao J, et al. (2007). "Gene expression profiling separates chromophobe renal ... 76 (1-3): 81-8. doi:10.1006/geno.2001.6610. PMID 11549320. "Entrez Gene: MAL2 mal, T-cell differentiation protein 2". Marazuela ... 332 (3): 675-87. doi:10.1016/S0022-2836(03)00944-6. PMID 12963375. Strausberg RL, Feingold EA, Grouse LH, et al. (2003). " ...
"A close association of the ganglioside-specific sialidase Neu3 with caveolin in membrane microdomains". J. Biol. Chem. 277 (29 ... Sialidase-3 is an enzyme that in humans is encoded by the NEU3 gene. This gene product belongs to a family of glycohydrolytic ... 56 (3): 420-9. doi:10.1016/j.metabol.2006.10.027. PMID 17292733. Wada T, Hata K, Yamaguchi K, Shiozaki K, Koseki K, Moriya S, ... J. 394 (Pt 3): 647-56. doi:10.1042/BJ20050737. PMC 1383714. PMID 16241905. Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T ...
The C-terminus of DLC1 is also known to interact with caveolin-1, although the biological significance of this interaction has ... caveolin-1, DLC-1, and NM23-H2 as putative antitumorigenic, progesterone-regulated genes for ovarian cancer cells by gene ... The detailed pathways by which DLC1 results in the cleavage of procaspase-3 and decrease in Bcl-2 levels require further ... DLC1 is responsible for inducing programmed cell death by at least two mechanisms: caspase-3-mediated apoptosis and Bcl-2 ...
... caveolin 1 MeSH D12.776.543.990.100.750 - caveolin 2 MeSH D12.776.543.990.100.875 - caveolin 3 MeSH D12.776.543.990.150.100 - ... toll-like receptor 3 MeSH D12.776.543.750.705.910.500.400 - toll-like receptor 4 MeSH D12.776.543.750.705.910.500.500 - toll- ... type 3 MeSH D12.776.543.750.060.116 - receptor, fibroblast growth factor, type 4 MeSH D12.776.543.750.060.124 - proto-oncogene ... erbb-3 MeSH D12.776.543.750.060.468 - receptor, igf type 1 MeSH D12.776.543.750.060.484 - receptor, insulin MeSH D12.776. ...
This gene codes for the Caveolin protein, which is a scaffolding membrane protein. This protein plays a role in lipid ... April 2008). "Association of a Homozygous Nonsense Caveolin-1 Mutation with Berardinelli-Seip Congenital Lipodystrophy". ... More recently, type 3 CGL was identified as a separate type of CGL, which was identified as a mutation in the CAV1 gene. Then, ... Types 3 and 4 are two different mutations but they share a common defective pathway. Medical diagnosis of CGL can be made after ...
There are two isoforms of caveolin-1: caveolin-1α and caveolin-1β, the latter lacking a part of the N-terminus. Caveolins are ... The expression pattern of caveolin-2 is similar to that of caveolin-1; it seems to be co-expressed with caveolin-1. The ... The caveolin gene family has three members in vertebrates: CAV1, CAV2, and CAV3, coding for the proteins caveolin-1, caveolin-2 ... Caveolin-1 has also been shown to play a role in the integrin signaling. The tyrosine phosphorylated form of caveolin-1 ...
... within caveolin-1 molecule. Molecules that interact with caveolin-1 contain caveolin-binding motifs (CBM). Caveolin GRCh38: ... Fra AM, Mastroianni N, Mancini M, Pasqualetto E, Sitia R (March 1999). "Human caveolin-1 and caveolin-2 are closely linked ... caveolin 2, PLD2, Bruton's tyrosine kinase, and SCP2. All these interactions are through a caveolin-scaffolding domain (CSD) ... caveolin. Caveolin binding negatively regulates the auto-activation of Src tyrosine kinases". The Journal of Biological ...
Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting ... "Entrez Gene: CAV3 caveolin 3". Hedley PL, Kanters JK, Dembic M, Jespersen T, Skibsbye L, Aidt FH, Eschen O, Graff C, Behr ER, ... Caveolin-3 is a protein that in humans is encoded by the CAV3 gene. Alternative splicing has been identified for this locus, ... Caveolin 3 has been shown to interact with a range of different proteins, including, but not limited to: DAG1, DYSF, EGFR, and ...
... has been shown to interact with Caveolin 1 and RAS p21 protein activator 1. GRCh38: Ensembl release 89: ... 1998). "Expression of caveolin-1 and -2 in differentiating PC12 cells and dorsal root ganglion neurons: caveolin-2 is up- ... Fra AM, Mastroianni N, Mancini M, Pasqualetto E, Sitia R (May 1999). "Human caveolin-1 and caveolin-2 are closely linked genes ... 2002). "Epithelial expression of caveolin-2, but not caveolin-1, is enhanced in the inflamed mucosa of patients with ulcerative ...
... co-immunoprecipitates with src kinases and caveolin". Pancreas. 21 (3): 219-25. doi:10.1097/00006676-200010000-00001. PMID ... 29 (3): 190-193. doi:10.1038/s41594-022-00729-3. PMC 8930769. PMID 35273390. PDB: 7P6R, 7P6S, 7P6T​ v t e (Articles with short ... 1491 (1-3): 376-80. doi:10.1016/S0167-4781(00)00057-9. PMID 10760606. Parker EM, Zaman MM, Freedman SD (2001). "GP2, a GPI- ... J. 277 (3): 879-81. doi:10.1042/bj2770879. PMC 1151326. PMID 1651706. Fukuoka S, Freedman SD, Scheele GA (1991). "A single gene ...
Caveolin-1 Influences LFA-1 Redistribution upon TCR Stimulation in CD8 T Cells (2017) Proximity of TCR and its CD8 coreceptor ... "Caveolin-1 Influences LFA-1 Redistribution upon TCR Stimulation in CD8 T Cells". Journal of Immunology. 199 (3): 874-884. doi: ... investigating the role of Caveolin-1 in T cell cholesterol homeostasis, integrin signaling and exosome complex biogenesis. In ... 3 January 2012). "Murine cytomegalovirus encodes a miR-27 inhibitor disguised as a target". Proceedings of the National Academy ...
Disruption of the maxi-K-caveolin-1 interaction alters current expression in human myometrial cells. Reprod Biol Endocrinol. ... 2019;34(3):323-331. doi:10.1177/0748730419844650 Reschke L, McCarthy R, Herzog ED, Fay JC, Jungheim ES, England SK. ... Funded by the March of the Dimes, England serves as Associate Program Director and Theme 3 Leader of the Prematurity Research ... 2002;192(1-2):1-6. doi:10.1016/s0303-7207(02)00136-3 Benkusky NA, Fergus DJ, Zucchero TM, England SK. Regulation of the Ca2+- ...
Caveolin-1) Associated With Human Pulmonary Arterial Hypertension". Circulation: Cardiovascular Genetics. 5 (3): 336-343. doi: ... 132 (3): 285-292. doi:10.1007/s00439-012-1249-0. ISSN 1432-1203. PMC 3570587. PMID 23138528. Austin Eric D.; Ma Lijiang; LeDuc ... 97 (3): 457-464. doi:10.1016/j.ajhg.2015.07.014. ISSN 0002-9297. PMC 4564988. PMID 26299366. Cheung, Yee Him; Gayden, Tenzin; ... 51 (3): 197-202. doi:10.1136/jmedgenet-2013-101989. ISSN 1468-6244. PMC 3955383. PMID 24385578. Chung, Wendy K.; Arkovitz, Marc ...
Caveolin 1 is dephosphorylated on tyrosine 14 in response to shear stress and PTPmu is hypothesized to catalyze this reaction. ... Caveolin 1 is a scaffolding protein enriched in endothelial cell junctions that is also linked to shear stress regulated ... Shin J, Jo H, Park H (2006). "Caveolin-1 is transiently dephosphorylated by shear stress-activated protein tyrosine phosphatase ... 34 (3): 453-67. doi:10.1016/j.mcn.2006.11.022. PMC 185529. PMID 17234431. Phillips-Mason PJ, Kaur H, Burden-Gulley SM, Craig SE ...
Feng X, Gaeta ML, Madge LA, Yang JH, Bradley JR, Pober JS (March 2001). "Caveolin-1 associates with TRAF2 to form a complex ... Caveolin 1, EDARADD, HIVEP3, IKK2, Low affinity nerve growth factor receptor, MAP3K14, MAP3K1, MAP3K7IP2, MAP4K2, MAP4K5, RANK ... "A phosphotyrosine-dependent protein interaction screen reveals a role for phosphorylation of caveolin-1 on tyrosine 14: ... 2 (3): 389-95. doi:10.1016/s1097-2765(00)80283-x. PMID 9774977. Hoeflich KP, Yeh WC, Yao Z, Mak TW, Woodgett JR (October 1999 ...
... repression antagonizes Sp1/sterol-responsive element-binding protein-induced transcriptional activation of caveolin-1 in ... 280 (3): 1901-10. doi:10.1074/jbc.M407941200. PMID 15531587. Neve B, Fernandez-Zapico ME, Ashkenazi-Katalan V, Dina C, Hamid YH ... 101 (3): 712-22. doi:10.1002/jcb.21228. PMID 17252542. S2CID 9730806. Niu X, Perakakis N, Laubner K, Limbert C, Stahl T, ... 8 (3): e60165. Bibcode:2013PLoSO...860165D. doi:10.1371/journal.pone.0060165. PMC 3610699. PMID 23555910. Mathison A, Grzenda A ...
"Amyloid beta-protein stimulates trafficking of cholesterol and caveolin-1 from the plasma membrane to the Golgi complex in ... 1 (3): FSO11. doi:10.4155/fso.15.9. PMC 5137966. PMID 28031886.. *^ a b Cummings J, Lee G, Mortsdorf T, Ritter A, Zhong K ( ... doi:10.1007/s12010-012-9549-3. PMC 3324686. PMID 22350870.. *^ Tharp WG, Sarkar IN (April 2013). "Origins of amyloid-β". BMC ... doi:10.1186/s40035-018-0139-3. PMC 6306008. PMID 30603085.. *^ Iliff JJ, Wang M, Liao Y, Plogg BA, Peng W, Gundersen GA, et al ...
PTGS2 has been shown to interact with caveolin 1. PTGS2 (COX-2) was discovered in 1991 by the Daniel Simmons laboratory[better ... "Colocalization and interaction of cyclooxygenase-2 with caveolin-1 in human fibroblasts". J. Biol. Chem. 276 (37): 34975-82. ... 73 (3-4): 141-62. doi:10.1016/j.plefa.2005.05.002. PMID 16005201. Wang Q1, He Y, Shen Y, Zhang Q, Chen D, Zuo C, Qin J, Wang H ... 51 (3): 575-85. doi:10.1194/jlr.M001719. PMC 2817587. PMID 19752399. Serhan CN (2005). "Lipoxins and aspirin-triggered 15-epi- ...
Clathrin and Caveolin 2-Dependent Manner". Cellular Microbiology. 11 (4): 629-644. doi:10.1111/j.1462-5822.2008.01279.x. PMC ... 107 (3): 121-125. ISSN 0003-9985. PMID 6687526. "History of Rocky Mountain Labs (RML) , NIH: National Institute of Allergy and ... In general, about 1-3% of the tick population carries R. rickettsii, even in areas where the majority of human cases are ... 5 (3): 252-76. doi:10.1016/j.ttbdis.2013.11.004. PMID 24556273. "Tickborne Rickettsial Diseases". Rocky Mountain Spotted Fever ...
... has been shown to interact with Caveolin 1 and peroxisomal receptor PEX5. GRCh38: Ensembl release 89: ENSG00000116171 - ... "Sterol carrier protein-2 directly interacts with caveolin-1 in vitro and in vivo". Biochemistry. 43 (23): 7288-306. doi:10.1021 ... 3 (2): 341-6. doi:10.1093/hmg/3.2.341. PMID 8004106. Seedorf U, Brysch P, Engel T, et al. (1994). "Sterol carrier protein X is ... Schroeder F, Butko P, Nemecz G, Scallen TJ (1990). "Interaction of fluorescent delta 5,7,9(11),22-ergostatetraen-3 beta-ol with ...
... with the help of adaptor proteins such as caveolin 1 or SHC1, which target the ER complexes to the plasma membrane. Activation ... 116 (3): 561-570. doi:10.1172/JCI27987. PMC 2373424. PMID 16511588. Diaz LK, Sneige N (January 2005). "Estrogen receptor ... These antibodies are commercially available from 3 commonly used autostain vendors- Dako, Leica, and Ventana, and in a study by ... of the ER complex causes increased kinase activity in phosphoinositide 3-kinases (PI3K) and mitogen-activated protein kinase ( ...
"Interaction of the immunoglobulin-like cell adhesion molecule hepaCAM with caveolin-1". Biochemical and Biophysical Research ... 3 (4): 334-6. doi:10.4161/cam.3.4.9246. PMC 2802741. PMID 19949308. Favre-Kontula L, Rolland A, Bernasconi L, et al. (April ... 39 (4): 580-6. doi:10.1016/S0168-8278(03)00359-3. PMID 12971969. Moh MC, Zhang C, Luo C, Lee LH, Shen S (July 2005). " ...
"The interaction between caveolin-1 and Rho-GTPases promotes metastasis by controlling the expression of alpha5-integrin and the ... 477 (3): 121-3. doi:10.1016/j.neulet.2010.04.046. PMID 20430066. S2CID 6740535. Wang Y, Bi L, Wang H, Li Y, Di Q, Xu W, Qian Y ... 3 (7): 723-34. doi:10.18632/oncotarget.547. PMC 3443255. PMID 22869051. Guo W, Ren D, Chen X, Tu X, Huang S, Wang M, Song L, ... 185 (3): 1155-61. doi:10.1016/0006-291x(92)91747-e. PMID 1378265. Law SF, Estojak J, Wang B, Mysliwiec T, Kruh G, Golemis EA ( ...
This region also contains a caveolin binding domain (CBD). The C-terminal end of the channel extends into a loop (residues 47- ... 68 (3): 1551-63. doi:10.1128/JVI.68.3.1551-1563.1994. PMC 236612. PMID 7508997. Rossman JS, Jing X, Leser GP, Lamb RA ( ... 85 (3): 1322-9. doi:10.1128/JVI.01367-10. PMC 3020500. PMID 21106743. Stewart SM, Pekosz A (January 2012). "The influenza C ... 10 (3): e0119115. Bibcode:2015PLoSO..1019115D. doi:10.1371/journal.pone.0119115. PMC 4358984. PMID 25768797. Deyde VM, Xu X, ...
... cell carcinomas and nonurothelial carcinomas of the urinary bladder differ in the promoter methylation status of the caveolin-1 ... 9 (3): 99-106. doi:10.1093/dnares/9.3.99. PMID 12168954. Nagase T, Kikuno R, Hattori A, et al. (2001). "Prediction of the ... 17 (1): 3-13. doi:10.3892/ijmm.17.1.3. PMID 16328005. Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based ... 2003). "AIP1 mediates TNF-α-induced ASK1 activation by facilitating dissociation of ASK1 from its inhibitor 14-3-3". J. Clin. ...
1998). "T-cadherin and signal-transducing molecules co-localize in caveolin-rich membrane domains of vascular smooth muscle ... 421 (3): 208-12. doi:10.1016/S0014-5793(97)01562-7. PMID 9468307. S2CID 26099158. Kremmidiotis G, Baker E, Crawford J, et al. ( ... 276 (3): 1240-7. doi:10.1006/bbrc.2000.3465. PMID 11027617. Ivanov D, Philippova M, Antropova J, et al. (2001). "Expression of ... 7 (3): 391-402. doi:10.1016/0896-6273(91)90291-7. PMID 1654948. Angst BD, Marcozzi C, Magee AI (15 February 2001). "The ...
... has been shown to interact with: CRK, Caveolin 1, Grb2, NCK1, NCK2, PDGFR-α, PTPN11, RAS p21 protein activator 1, SHC1 ... Yamamoto M, Toya Y, Jensen RA, Ishikawa Y (March 1999). "Caveolin is an inhibitor of platelet-derived growth factor receptor ... 144 (3): 377-92. doi:10.1309/AJCPMORR5Z2IKCEM. PMID 26276769. Arber DA, Orazi A, Hasserjian R, Thiele J, Borowitz MJ, Le Beau ... Braverman LE, Quilliam LA (February 1999). "Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter ...
... cell by binding to receptors on the surface of the cell and entering it by endocytosis mediated by either clathrin or caveolin- ... 20 (3): 209-18. doi:10.1111/j.1365-2893.2012.01655.x. PMID 23383660. S2CID 205356299. Drexler JF, Geipel A, König A, Corman VM ... 57 (3): 908-16. doi:10.1002/hep.26079. PMID 22987324. Bouckaert R, Simons BC, Krarup H, Friesen TM, Osiowy C (2017). "Tracing ... 17 Suppl 3: S20-3. doi:10.1016/s0168-8278(05)80419-2. PMID 8509635. Beck J, Nassal M (January 2007). "Hepatitis B virus ...
... of caveolin 1 and caveolin 2. Caveolin is, paradoxically, tumour-promoting in IBC. NF-κB pathway activation overexpression may ... Caveolin 1 and caveolin 2 are overexpressed, and may contribute to tumour cell motility E-cadherin is overexpressed; ... "Overexpression of caveolin-1 and -2 in cell lines and in human samples of inflammatory breast cancer" (PDF). Breast Cancer ... IBC is typically diagnosed in one of these stages: Stage IIIB - at least 1/3 of the skin of the breast is affected, and cancer ...
Razani B, Lisanti MP (2001). "Two distinct caveolin-1 domains mediate the functional interaction of caveolin-1 with protein ... 327 (3): 609-18. doi:10.1016/S0022-2836(03)00093-7. PMID 12634056. Herberg FW, Maleszka A, Eide T, Vossebein L, Tasken K (April ... 327 (3): 609-18. doi:10.1016/S0022-2836(03)00093-7. PMID 12634056. Steen RL, Beullens M, Landsverk HB, et al. (2004). "AKAP149 ...
Hyaluronan is tethered to cell surfaces by CD44 which are anchored in caveolin-rich lipid rafts. Cleavage and further ... 254 (3): 497-504. doi:10.1046/j.1432-1327.1998.2540497.x. PMID 9688259. Capello M, Ferri-Borgogno S, Cappello P, Novelli F ( ... 14 (3): 321-4. doi:10.1111/j.1743-6109.2006.00127.x. PMID 16808811. S2CID 30136711. Stern R (August 2008). "Hyaluronidases in ... 30 (3): 325-339. doi:10.1016/j.nic.2020.05.003. PMID 32600634. S2CID 220272658. University Hospital Tuebingen (2015-01-30). " ...
Wary, K.K.; Mariotti, A.; Zurzolo, C.; Giancotti, F.G. (1998). "A requirement for caveolin-1 and associated kinase Fyn in ... Wickstrom, S.A.; Alitalo, K.; Keski-Oja, J. (2002). "Endostatin associates with integrin alpha5beta1 and caveolin-1, and ... Endostatin binding and clustering of integrins causes co-localization with caveolin-1 and activates non-receptor tyrosine ... 275 (3): 1521-4. doi:10.1074/jbc.275.3.1521. PMID 10636838. Ma, L; Hollenberg, M.D.; Wallace, J.L. (2001). "Thrombin-induced ...
Carman CV, Lisanti MP, Benovic JL (Mar 1999). "Regulation of G protein-coupled receptor kinases by caveolin". Journal of ... 162 (3): 401-8. doi:10.1677/joe.0.1620401. PMID 10467231. Okochi M, Walter J, Koyama A, Nakajo S, Baba M, Iwatsubo T, Meijer L ... 298 (3): 1243-51. PMID 11504827. Blaukat A, Pizard A, Breit A, Wernstedt C, Alhenc-Gelas F, Muller-Esterl W, Dikic I (Nov 2001 ... 521 (1-3): 140-4. doi:10.1016/S0014-5793(02)02858-2. PMID 12067742. S2CID 29467488. Portal: Biology v t e (Articles with short ...
... directly binds caveolin-1". FEBS Letters. 508 (1): 49-52. doi:10.1016/S0014-5793(01)03020-4. PMID 11707266. S2CID 7887096. ... 3: 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. Moreno CS, Park S, Nelson K, Ashby D, Hubalek F, Lane WS, Pallas DC ... 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. v t e (Genes on human chromosome 2, All stub articles, Human ... network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 ...
2009). "Caveolin-1 scaffold domain interacts with TRPC1 and IP3R3 to regulate Ca2+ store release-induced Ca2+ entry in ... 9 (3): 264-6. doi:10.1038/gene.2008.12. PMID 18340361. Wu Z, Bowen WD (2008). "Role of sigma-1 receptor C-terminal segment in ... 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. Roach JC, Deutsch K, Li S, et al. (2006). " ... 190 (3): 285-96. doi:10.1083/jcb.201003144. PMC 2922655. PMID 20696704. Kline CF, Cunha SR, Lowe JS, et al. (2008). "Revisiting ...
"Ligand-independent activation of oestrogen receptor alpha by caveolin-1". The Biochemical Journal. 359 (Pt 1): 203-210. doi: ... 3 (6): 903-910. doi:10.1016/S1534-5807(02)00360-X. PMID 12479814. Ogawa S, Inoue S, Watanabe T, Hiroi H, Orimo A, Hosoi T, et ... 14 (3): 369-381. doi:10.1210/mend.14.3.0432. PMID 10707955. Townson SM, Dobrzycka KM, Lee AV, Air M, Deng W, Kang K, et al. ( ... 3 (1): 30-37. doi:10.1038/35050532. PMID 11146623. S2CID 23477845. Lee SK, Anzick SL, Choi JE, Bubendorf L, Guan XY, Jung YK, ...
Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting ... "Entrez Gene: CAV3 caveolin 3". Hedley PL, Kanters JK, Dembic M, Jespersen T, Skibsbye L, Aidt FH, Eschen O, Graff C, Behr ER, ... Caveolin-3 is a protein that in humans is encoded by the CAV3 gene. Alternative splicing has been identified for this locus, ... Caveolin 3 has been shown to interact with a range of different proteins, including, but not limited to: DAG1, DYSF, EGFR, and ...
Caveolin-1 and -2 are coexpressed and form heterooligomers in nonmuscle cells whereas caveolin-3 is muscle specific and forms ... Aberrant dysferlin trafficking in cells lacking caveolin or expressing dystrophy mutants of caveolin-3. Hum. Mol. Genet. 15:129 ... Identification of peptide and protein ligands for the caveolin-scaffolding domain. Implications for the interaction of caveolin ... The interaction between caveolin and lipid-modified proteins is mediated by a specific caveolin-binding motif on the target ...
A family of integral membrane proteins named caveolins (mainly caveolin-1 and caveolin-3 in the myocardium) and a protein ... Keywords: adiponectin; caveolin 1; caveolin 3; diabetic cardiomyopathy; insulin signal pathway; oxidative stress. ... The importance of caveolin as a target in the prevention and treatment of diabetic cardiomyopathy Front Immunol. 2022 Nov 2;13: ... Weiyi Xia 1 2 , Xia Li 3 , Qingping Wu 3 , Aimin Xu 4 , Liangqing Zhang 1 , Zhengyuan Xia 1 4 ...
CAV3: caveolin 3. *CAVIN1: caveolae associated protein 1. *CBFB: core-binding factor subunit beta ...
Caveolin-3 regulates myostatin signaling. Mini-review. Y. Ohsawa, T. Okada, A. Kuga, S. Hayashi, T. Murakami, K. Tsuchida, S. ... Caveolin-3 regulates myostatin signaling. Mini-review. In: Acta Myologica. 2008 ; Vol. 27, No. JULY. pp. 19-24. ... Ohsawa, Y., Okada, T., Kuga, A., Hayashi, S., Murakami, T., Tsuchida, K., Noji, S., & Sunada, Y. (2008). Caveolin-3 regulates ... Ohsawa, Y, Okada, T, Kuga, A, Hayashi, S, Murakami, T, Tsuchida, K, Noji, S & Sunada, Y 2008, Caveolin-3 regulates myostatin ...
Mutation of the caveolin-3 gene (CAV3) causes this disorder. [22] See image below. ... The gene location is 3p25, and the gene product is caveolin-3. Caveolin-3 is a muscle-specific protein related to the caveolae ... The sarcolemmal proteins dysferlin and caveolin-3 interact in skeletal muscle. Hum Mol Genet. 2001 Aug 15. 10(17):1761-6. [QxMD ... Mutations in all sarcoglycans, in dysferlin, and in caveolin-3, as well as mutations that cause abnormal glycosylation of alpha ...
Patients with LGMD1C have reduced staining for caveolin-3 by immunohistochemistry and Western blot. There may also be reduced ... Mutations in all sarcoglycans, dysferlin, and caveolin-3, as well as mutations that cause abnormal glycosylation of alpha- ... Loss of staining for both bands occurs in about 25% of cases and is highly specific for a calpain-3 mutation. About 25% of ... Patients with LGMD2A have reduced staining for calpain-3 by Western blot. Reduction or loss of staining for the 60kD band is ...
ASM2; Caveolin-3; annexin I. [108-113]. Epithelial cells. Caveolae-. mediated endocytosis. PFTs1. ASM; Caveolin-3; annexin I. [ ... stimulates Arp2/3s actin nucleation activity [91], accelerating production of actin networks critically involved in actomyosin ... While caveolin-3-deficient mice exhibit robust muscular degeneration [43], the relative contribution of caveolae in protection ... Caveolin-3 deficiency causes muscle degeneration in mice. . Hum Mol Genet, 2000. 9(20): pp. 3047-54.. ...
Caveolin-3 (15). * CDK1+Cdk7+Cdk9+CDK12+XPD+TrkA+KDS+FER+MELK+MAP4K1/HPK1+Wee1+GSK3 beta+Aurora B+Aurora A+CDK2+FAK+GSK (1). ... DNA PKcs+mTOR+PLK1+PI3 Kinase p110 beta+PI 3 Kinase p110 delta+PI 3 Kinase p85 alpha+GSK3 beta+PIK3C (1). ... Get 4 for 3 with code 4FOR3-E5TN2. Click here for terms and conditions. ...
Epigallocatechin-3-gallate-mediated cardioprotection by Akt/GSK-3β/caveolin signalling in H9c2 rat cardiomyoblasts Authors ( ... Caspase 3 involves in neuroplasticity, microglial activation and neurogenesis in the mice hippocampus after intracerebral ...
caveolin 1. acts_upstream_of_or_within. ISO. MGI:2180372 (MGI:3783955,PMID:17293479). RGD. PMID:17293479. MGI:3783955. NCBI chr ... caveolin 3. IEP. RGD. PMID:29438664. RGD:126925221. NCBI chr 4:145,582,168...145,598,142 Ensembl chr 4:145,582,060... ... Ensembl chr 3:141,253,523...141,303,479 G. Bcl2l2. Bcl2-like 2. IEP. RGD. PMID:10724126. RGD:14394498. NCBI chr15:28,346,449... ... Ensembl chr 3:108,388,245...108,413,236 G. Per2. period circadian regulator 2. involved_in. ISS. ISO. GO_REF:0000024. (MGI: ...
... for K12959 both genes hit to caveolin and caveolin-like proteins in other salmonids (Supplementary Data 3). WFS12 and WFS10 are ... 3: Excess allele-sharing is widespread between whitefish species both within and between lake systems.. ... 3, pink box). The strongest signals of excess allele-sharing with C. nobilis came from the Albeli species C. albellus in ... 3). This also confirms, and clarifies, the results of other studies which suggested that the evolution of C. acrinasus involved ...
Expression of Caveolin-3 in the Muscle Cell and Tissue Kwon BS, Lee SJ, Hyun JK, Jun DJ, Joo HW, Kim BH, Shin DH ... OBJECTIVE: Caveolae are the microdomain of the plasma membrane that have been implicated in signal transduction and caveolin is ... 3. Recent Advances in Skeletal Muscle Stem Cells for Duchenne Muscular Dystrophy Treatment Yang JY, Jeong J ...
P. L. Cameron, J. W. Ruffin, R. Bollag, H. Rasmussen, and R. S. Cameron, "Identification of caveolin and caveolin-related ... T. M. Williams and M. P. Lisanti, "The caveolin proteins," Genome Biology, vol. 5, no. 3, Article ID 214, 2004. ... caveolin), is distinguished by flask-shaped invaginations of the plasma membrane [44]. These platforms serve as signaling ... 3. Membrane Lipid Rafts and ASD. Studies on cholesterol and lipid organization in disease have led to progress in understanding ...
2.67 ᠰ.46; P , 0.05]) and mitochondrial caveolin levels (n = 16 per group; [caveolin-1: 0.87 ᠰ.18 vs. 1.89 ᠮ19; P , 0.05]; [ ... caveolin-1: 1.78 ᠰ.12 vs. 3.53 ᠰ.77; P , 0.05]; [caveolin-3: 1.68 ᠰ.29 vs. ... Trafficking of caveolin to mitochondria is essential for adaptation to cellular stress though the trafficking mechanisms remain ... Trafficking of caveolin to mitochondria is essential for adaptation to cellular stress though the trafficking mechanisms remain ...
Le, P. U., Guay, G., Altschuler, Y. & Nabi, I. R. Caveolin-1 is a negative regulator of caveolae-mediated endocytosis to the ... Webb, A. et al. EHBP1 and EHD2 regulate Dll4 caveolin-mediated endocytosis during blood vessel development. bioRxiv (2020) doi: ... Porta, J. C. et al. Molecular architecture of the human caveolin-1 complex. bioRxiv (2022) doi:10.1101/2022.02.17.480763. ... Yamaguchi, T. et al. ROR1 sustains caveolae and survival signalling as a scaffold of cavin-1 and caveolin-1. Nat. Commun. 7, ( ...
Caveolin-3 null mice show a loss of caveolae, changes in the microdomain distribution of the dystrophin-glycoprotein complex, ... For example, Limb-girdle muscular dystrophy 1C (LGMD-1C) is caused by mutations in caveolin 3 (CAV3), which is believed to be ... Transgenic overexpression of caveolin-3 in skeletal muscle fibers induces a Duchenne-like muscular dystrophy phenotype. Proc. ... Mutations in the caveolin-3 gene cause autosomal dominant limb-girdle muscular dystrophy. Nat. Genet ...
Journal Article] Overexpression of P104L mutant caveolin-3 in mice develops hypertrophic cardiomyopathy with enhanced ... Journal Article] Overexpression of P104L mutant caveolin-3 in mice develops hypertrophic cardiomyopathy with enhanced ...
Caveolin 3 related distal myopathy Current Synonym true false 3075994019 Distal myopathy, Tateyama type Current Synonym true ... Caveolin 3 related distal myopathy (disorder). Code System Preferred Concept Name. Caveolin 3 related distal myopathy (disorder ...
McArdles syndrome, carnitine palmitoyltransferase deficiency, Beckers syndrome, and disorders of caveolin-3 may be silent ... 3. Zyl-Smit R, Mills P, Vogelpoel L. Unrecognized acute renal failure following the comrades marathon. S Afr Med J 2000;90:39- ... 3. Zyl-Smit R, Mills P, Vogelpoel L. Unrecognized acute renal failure following the comrades marathon. S Afr Med J 2000;90:39- ... Three of the 4 runners reported vomiting in the period between race finish and hospital admission, while patient 3 reported ...
Human molecular genetics, 7, 5. Caveolin-3 in muscular dystrophy McNally EM ; Moreira de Sà E ; Duggan DJ ; Bonnemann CG ; ... Paternal inheritance or different mutations in maternally related patients occur in about 3% of Duchenne familial cases ... Neuromuscular disorders : NMD, 8, 3. Sarcoglycanopathies are responsible for 68 % of severe autosomal recessive limb-girdle ...
Mutation of the caveolin-3 gene (CAV3) causes this disorder. [22] See image below. ... The gene location is 3p25, and the gene product is caveolin-3. Caveolin-3 is a muscle-specific protein related to the caveolae ... The sarcolemmal proteins dysferlin and caveolin-3 interact in skeletal muscle. Hum Mol Genet. 2001 Aug 15. 10(17):1761-6. [QxMD ... Mutations in all sarcoglycans, in dysferlin, and in caveolin-3, as well as mutations that cause abnormal glycosylation of alpha ...
The sarcolemmal proteins dysferlin and caveolin-3 interact in skeletal muscle. Hum Mol Genet. 2001 Aug 15. 10(17):1761-6. [QxMD ... Mutations in all sarcoglycans, in dysferlin, and in caveolin-3, as well as mutations that cause abnormal glycosylation of alpha ... Familial isolated hyperCKaemia associated with a new mutation in the caveolin-3 (CAV-3) gene. J Neurol Neurosurg Psychiatry. ... Mutations in all sarcoglycans, in dysferlin, and in caveolin-3, as well as mutations that cause abnormal glycosylation of alpha ...
2015) The caveolin-cavin system plays a conserved and critical role in mechanoprotection of skeletal muscle Journal of Cell ... 2015b) Molecular characterization of Caveolin-induced membrane curvature Journal of Biological Chemistry 290:24875-24890. ... 1996) Expression of caveolin-3 in Skeletal, cardiac, and smooth muscle cells Caveolin-3 is a component of the sarcolemma and co ... Note that, Caveolin1 (Uniprot accession: Q6YLH8) and Caveolin (isoform unassigned; Uniprot accession: A1L1S3) were detected in ...
Modest Effects of Lipid Modifications on the Structure of Caveolin-3. June 26th, 2014 by Ji-Hun Kim, Dungeng Peng, Jonathan P. ...
Keywords: Caveolae, caveolin-1, breast cancer, prostate cancer, melanoma, metalloproteins, MMP-2, MMP-9, estrogen receptor, ... Role of Caveolin-1 in Cancer and Therapeutic Implications. Author(s): Gloria Bonuccelli, Philippe G. Frank and *Breakthrough ... Role of Caveolin-1 in Cancer and Therapeutic Implications, Cutting Edge Therapies for Cancer in the 21st Century (2014) 1: 3. ... These organelles are characterized by the presence of the caveolin-1 (Cav-1) protein that plays an important role in the ...
Patients with LGMD1C have reduced staining for caveolin-3 by immunohistochemistry and Western blot. There may also be reduced ... Mutations in all sarcoglycans, dysferlin, and caveolin-3, as well as mutations that cause abnormal glycosylation of alpha- ... Loss of staining for both bands occurs in about 25% of cases and is highly specific for a calpain-3 mutation. About 25% of ... Patients with LGMD2A have reduced staining for calpain-3 by Western blot. Reduction or loss of staining for the 60kD band is ...
b, NgRL1 is in multiple membrane fractions, with a small proportion in the caveolin-positive detergent-insoluble fraction. c, ... Caveolin was detected with a rabbit polyclonal antibody (Upstate Biotechnology, Lake Placid, NY). ... In contrast, the vast majority of chimeric NgRL1 localizes to the caveolin-negative fractions, and the small proportion ... WTNgR is found almost exclusively in the caveolin-positive detergent-insoluble fraction. ...
Caveolin 3. Creatine phosphokinase, elevated serum (formerly HyperCKemia, iodiopathic) (1.27, 6.6, 5.24, 6.7, 4.13, 10.132, ... Cardiomyopathy, familial hypertrophic, 3 (10.3, 10.60, 10.100). 115196. TPM1 (15q22). Tropomyosin 1 (alpha). ... charcot-marie-tooth disease, x-linked recessive, 3 (14.38). 302802. 78 kb Chro8 insertion (Xq26). 78 kb inter-chromosomal ...
  • Regarding CAV1 (caveolin 1), Sekhar et al. (
  • Specifically, downregulation of the nicotinic cholinergic receptor subunit beta-2 gene (Chrnb2), monoaminergic enzymes catechol-O-methyltransferase (Comt) and dopa decarboxylase (Ddc), chloride channels Clcn4 and Clcn5, scaffolding protein caveolin 1 (Cav1), cortical development/cytoskeleton element lissencephaly 1 (Lis1) and intracellular signaling cascade member adenylate cyclase 3 (Adcy3) was observed in pS422-immunreactive nbM neurons in CTE patients. (
  • Type 3 BSCL is involved in mutation in the CAV1 gene which is responsible for the creation of the Caveolin protein which plays a role in lipid regulation (Parton, Simons, 2007). (
  • Caveolin-3 is a protein that in humans is encoded by the CAV3 gene. (
  • Other mutations in Caveolin causes Long QT Syndrome or familial hypertrophic cardiomyopathy, although the role of Cav3 in Long QT syndrome has recently been disputed. (
  • Mutation of the caveolin-3 gene ( CAV3 ) causes this disorder. (
  • We report the first case of a heterozygous T78M mutation in the caveolin-3 gene (CAV3) associated with rippling muscle disease and proximal myopathy. (
  • Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. (
  • Caveolin 3 has been shown to interact with a range of different proteins, including, but not limited to: DAG1, DYSF, EGFR, and RYR1. (
  • There are many proteins that associate with caveolin-3, including ion channels and exchangers. (
  • Disruption of interactions between caveolin-3 and its associated binding proteins has been shown to affect LTCC. (
  • This occurs by changing the PKA-mediated phosphorylation of caveolin-3-associated binding proteins, causing negative down-stream effects on LTCC activity. (
  • A family of integral membrane proteins named caveolins (mainly caveolin-1 and caveolin-3 in the myocardium) and a protein hormone adiponectin (APN) have all been shown to be important for maintaining normal insulin signaling. (
  • If initial targeted genetic testing (either single gene or a panel of LGMDs) is negative, a muslce biopsy showld be obtained to look at the immunohistochemical staining patterns using antibodies directed at known disease associated proteins (e.g. dystrophin, sarcoglycans, merosin, α-dystroglycan, dysferlin, cveloin-3, etc) and to look for distinguishing features (e.g. rimmed vacuoles, myofibrillar myopathy). (
  • Gi proteins are involved in trafficking caveolin to mitochondria to enhance stress resistance. (
  • They concentrate specific lipids including cholesterol, sphingolipids, and PI(4,5)P 2 , supporting the clustering of distinct proteins and signaling molecules 2 , 3 . (
  • The caveolin proteins (caveolin1-3 in mammals) and cavins (cavin1-4) are central for organelle formation. (
  • Using biochemistry and fluorescence resonance energy transfer-based approaches, we now show that a family of related proteins, PTRF/Cavin-1, serum deprivation response (SDR)/Cavin-2, SDR-related gene product that binds to C kinase (SRBC)/Cavin-3, and muscle-restricted coiled-coil protein (MURC)/Cavin-4, forms a multiprotein complex that associates with caveolae. (
  • In microglia, podosomes were also enriched in phosphotyrosine residues and three tyrosine-kinase-regulated proteins: tyrosine kinase substrate with five Src homology 3 domains (Tks5), phosphorylated caveolin-1, and Nox1 (nicotinamide adenine dinucleotide phosphate oxidase 1). (
  • The peroxynitrite anion (ONOO - ) produced during this process can nitrate several biomolecules, such as proteins, lipids, and DNA, to generate 3-nitrotyrosine (3-NT), which further induces cell death. (
  • The focus of this project is to investigate the role of 14-3-3 proteins in the assembly and maintenance of desmosomal adhesion and their influence on cell migration and invasion. (
  • Among proteins markedly upregulated in HF kidneys were proteins critical for formation of caveoleae - the main caveolar structural protein caveolin 1 , three adapter proteins cavins 1, 2,3 and two additional caveoleae components - EH-domain containing proteins 2 and 4. (
  • Additionally, 4HR downregulated the expression of oncogenesis-related proteins, i.e., a unfavorable regulator of apoptosis (survivin, 14.8 at eight h), an anti-adhesive glycoprotein that contributes to tumor improvement and metastasis (mucin four, five.7 at 24 h), and a potent oncogene that binds to 14-3-3 (YAP, 20.6 at eight h). (
  • [2] [3] Dystrophin binds to actin of the cytoskeleton , and also to proteins in the extracellular matrix . (
  • through a brief hairpin hydrophobic protrude and domains to the cytoplasm, where they are able to bind and impact the experience of several proteins companions a caveolin scaffolding domains (CSD) [6, 7]. (
  • Cav-1, Cav-2 and Cav-3 are the main structural proteins of and are codified by three different genes that have a high degree of homology [1, 2] (Table 1). (
  • Proteins such as Caveolin-1 (pink), tetraspanins (blue) or flotillins (violet) define these protein islands and thereby regulate the functioning of the immune system. (
  • This paradox prompted a re-evaluation of its function in angiogenesis (10) and was the impetus for our research concentrating on the proteins expression changes caused by elimination from the 3 integrin subunit in mouse cells. (
  • Within the dedication of P-gp (170 kD) and caveolin-1 (22 kD), crude membrane proteins (50 g) had been put through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) utilizing a stepwise gradient polyacrylamide gel (3.5% (w/v) stacking gel and 7.5% (upper zone) and 10% (bottom zone) separation gel. (
  • Oxygen tension and cytokines have been shown to influence trophoblast VEGF expression, suggesting that this particular family of angiogenic proteins plays an important role in placental angiogenesis [3]. (
  • In cardiac myocytes, caveolin-3 negatively regulates ATP-dependent potassium channels (KATP) localized in caveolae. (
  • Caveolin-3 regulates myostatin signaling. (
  • Dive into the research topics of 'Caveolin-3 regulates myostatin signaling. (
  • Phosphocaveolin-1 regulates a positive feedback loop that responds to mechanical stress to induce caveola biogenesis by relieving Egr1 transcriptional inhibition of caveolin-1 and cavin-1. (
  • Glutamine uptake via SNAT6 and Caveolin regulates glutamine-glutamate (2021) International Journal of Molecular Sciences 22(3):1167. (
  • Caveolin-1 Regulates the P2Y2 Receptor Signaling in Human 1321N1 Astrocytoma Cells. (
  • Caveolin-1 is a membrane protein which regulates endothelial nitric oxide synthase (eNOS) activity which is responsible for NO generation and cellular insulin-signaling. (
  • Caveolin-1 regulates lung cancer stem-like cell induction and p53 inactivation in carbon nanotube-driven tumorigenesis. (
  • This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. (
  • Mutations have been identified in the caveolin-3 gene that result in cardiomyopathies. (
  • The gene location is 3p25, and the gene product is caveolin-3. (
  • These studies were the first in vivo demonstrations showing that caveolin-1 could function as a tumor suppressor gene. (
  • It was shown that, depending on the histogenesis of the tumor, caveolin-1 may function as a tumor suppressor gene as well as an oncogene. (
  • Nuclear Galectin-3 can modulate gene expression, while cytosolic Galectin-3 can inhibit apoptosis and can participate in exocytosis, Caveolin-mediated endocytosis, and macrophage-mediated clearance of apoptotic cells (5-7). (
  • The top 10 hub genes and top 3 gene interaction modules were selected from the PPI network. (
  • Importantly, we will use cell and mouse models lacking the caveolin gene to determine the involvement of caveolae in inflammatory outcome. (
  • The MT 3 gene is additionally silent in cell lines derived through the UROtsa mother or father which have been malignantly transformed by either Cd two or As three. (
  • These findings recommend that MT 3 gene expression is silenced in each the parental UROtsa cells and also the Cd 2 and As three transformed counterparts by way of a mechanism involving histone modification. (
  • Marrakesh Treaty will make into download SPRING 3 Для профессионалов on September 30, 2016. (
  • 2016. Theaflavin-3,3'-digallate decreases human ovarian carcinoma OVCAR-3 cell-induced angiogenesis via Akt and NOTCH-1 pathways, not via MAPK pathways. (
  • Similarly, dominant-negative genotypes for caveolin-3 increase cardiac hypertrophy, whereas increased expression of caveolin-3 inhibits the ability of the heart to hypertrophy, implicating caveolin-3 as a negative regulator of cardiac hypertrophy. (
  • Caveolin-1 Deficiency Inhibits the Basolateral K+ Channels in the Distal Convoluted Tubule and Impairs Renal K+ and Mg2+ Transport. (
  • This study aimed to investigate whether low SS inhibits endothelial mitophagy via caveolin-1 (Cav-1)/miR-7-5p/Sequestosome 1 (SQSTM1) signaling pathway. (
  • Herein we review caveolin-3 suppressing of activation of type I myostatin receptor, thereby inhibiting subsequent intracellular signaling. (
  • The authors hypothesized that G protein-coupled receptor/inhibitory G protein (Gi) activation leads to caveolin trafficking to mitochondria. (
  • Further, both inositol 1,4,5-trisphosphate receptor-3 and caveolin-1 were immunoprecipitated with ΔTrp1α and Trp1α. (
  • We examined expression of the cysteine protease cathepsin B and the serine protease urokinase plasminogen activator (uPA), its receptor uPAR and caveolin-1 in two IBC cell lines: SUM149 and SUM190. (
  • The contents of 5-HT2A receptor and of caveolin-1 were unaffected by cholesterol extraction, according to Western blots. (
  • Similarly, caveolin-1 overexpression attenuated basal and insulin-stimulated NO synthesis along with impaired insulin-dependent activation of AKT and eNOS, with no effect on insulin-stimulated ERK1/2 phosphorylation and endothelin-1 transcription. (
  • Src-induced phosphorylation of caveolin-2 on tyrosine 19. (
  • Formulation, 3 no correlations were observed between testosterone exposure trenbolone as the base hormone is an extremely powerful hormone antibody Arrays Glycobiology Arrays Protein Arrays Phosphorylation Arrays RayPlex Bead Arrays. (
  • Caveolin-3 is a muscle-specific protein related to the caveolae, which are the invaginations of the plasma membrane. (
  • They are also widely expressed in human tissues, primarily localized within the plasma membrane, have similar topology, capability for oligomerization and actively participate in the regulation of signaling pathways, some of which intersect with caveolin-dependent pathways. (
  • This complex can constitutively assemble in the cytosol and associate with caveolin at plasma membrane caveolae. (
  • In the Golgi area, a dipalmitoylated GFP chimera colocalized with cholesterol and GM 1 at the plasma membrane and with caveolin. (
  • The depletion of cholesterol from the plasma membrane resulted in the emergence of PACSIN2-localized tubules with caveolin-1 at their tips, suggesting that cholesterol blocking inhibited the prominent membrane tubulation by PACSIN2. (
  • Mutations in all sarcoglycans, in dysferlin, and in caveolin-3, as well as mutations that cause abnormal glycosylation of alpha-dystroglycan, can result in limb-girdle muscular dystrophy. (
  • Several of these mutations influence caveolin-3 function by reducing the expression of its cell-surface domains. (
  • Mutations resulting in loss-of-function of caveolin-3 cause cardiac myocyte hypertrophy, dilation of the heart, and depression of fractional shortening. (
  • Loss of caveolin-3, resulting from dominant negative mutations of caveolin-3 causes autosomal dominant limb-girdle muscular dystrophy (LGMD) 1C and autosomal dominant rippling muscle disease (AD-RMD). (
  • Mutations in the 3 major LQTS-susceptibility genes (KCNQ1, KCNH2 and SCN5A) account for approximately 60-75% of congenital LQTS cases with a strong clinical phenotype, while the 15 other minor genes contribute approximately an additional 5% (Tester et al. (
  • Enhanced myostatin signaling by caveolin-3 mutation in human may contribute to the pathogenesis of LGMD1C. (
  • Here, we describe precision needle injury, tail-notochord amputation, and chemical inhibition of caveolin that trigger a wound-specific wt1b expression response in the notochord sheath cell subpopulation. (
  • Specifically, disruption of caveolin-3 decreases the basal and b2-adrenergic-stimulated opening probabilities of LTCC. (
  • In contrast, there was MTF 1 binding to MREa and MREb of the MT three pro moter within the Cd two and As 3 transformed cell lines beneath basal ailments, by using a even further maximize in binding fol lowing treatment with MS 275. (
  • In contrast, the Cd two and As 3 transformed cell lines had been shown to have improved binding of MTF 1 to MREc of your MT 3 promoter beneath the two basal disorders with no improve in interac tion following therapy with MS 275. (
  • In contrast, MREe, f, g from the MT 3 promoter had been capable to bind MTF 1 below basal conditions, which was greater following treat ment with MS 275. (
  • Sucrose density fractionation and Western blot analysis demonstrated that an active form of cathepsin B localizes to caveolar fractions along with caveolin-3, annexin-VII, I2-dystroglycan and dystrophin. (
  • Immunoblotting evaluation of caveolin-1 in fractions was performed as defined below. (
  • In the caveolin-1 u2013containing fractions, enrichment of 5-HT2A receptors but not u03b1 1 -adrenergic receptors was found enrichment, but not u03b1 1 -adrenergic receptors, suggesting localization of the former to caveolae. (
  • IBC patient biopsies were examined for expression of cathepsin B and caveolin-1. (
  • A statistically significant co-expression of cathepsin B and caveolin-1 was found in IBC patient biopsies, thus validating our in vitro data. (
  • steady heterologous cells transfected with 3 integrin, where in fact the intracellular activity and expression of cathepsin B was more affordable in comparison to control cells. (
  • Hence, we examined the effects of IH on caveolin-1, eNOS, and endothelin-1 in human coronary artery endothelial cells in the context of IR. (
  • The specific roles of ICAM-1, Src, eNOS, and caveolin-1 regulated signaling in endothelial cells, isolated lungs, and whole animals are being studied with regards to normal lung physiology and disease mechanisms. (
  • He has published several sentinel papers on caveolin-1 regulation of endothelial cell function via downstream associated Src and eNOS signaling pathways, and the roles of oxidative stress signals in endothelial hyperperpermeability and inflammatory syndromes. (
  • In vitro exposure of caveolae-enriched membranes to ROS dissociated caveolin more quickly than eNOS from the membranes. (
  • These results show that cholesterol therapy improves eNOS expression, while ROS treatment reduces eNOS expression and the association of eNOS with caveolin in caveolae-enriched membranes. (
  • These pharmaceuticals inhibited clathrin-mediated endocytosis, caveolin-mediated endocytosis and actin-dependent pinocytosis/phagocytosis respectively. (
  • 3. In vitro activity: Phenylbutyrate in prostate cancer cells by attenuating the apoptosis antagonist Bcl-X(L), double-strand break repair protein DNA-dependent protein kinase, prostate progression marker caveolin-1 and proangiogenesis The expression level of vascular endothelial growth factor induces apoptosis of prostate cancer cells. (
  • However, other investigators have shown that certain cancer cells show up-regulation of caveolin-1 that results in more metastatic and aggressive tumor like in bladder, thyroid, and prostate carcinomas. (
  • As a result caveolin-1 can affect cell proliferation, programmed cell death, migration and other processes important for tumor transformation and progression. (
  • Caveolin-1 was initially hypothesized to be a tumor suppressor in breast cancer [ 13 ]. (
  • We previously hypothesized that the high levels of caveolin-1 expression in bladder, colon, esophageal and prostate cancers promote cell surface proteolytic events that lead to extracellular matrix (ECM) degradation and tumor invasion [ 17 ]. (
  • In this regard, it is noteworthy that caveolin is a component of several intracellular vesicle populations, caveolin-1 is required for lipid droplets formation, and all forms of caveolins. (
  • We hypothesize that cholesterol dysfunction may lead to ASD by three mechanisms working in concert during brain development: (1) impaired sonic hedgehog patterning, (2) alterations in membrane lipid raft structure and protein function resulting in abnormal synaptic plasticity, and (3) impaired neurosteroid synthesis. (
  • Cavin-4 is expressed predominantly in muscle, and its distribution is perturbed in human muscle disease associated with Caveolin-3 dysfunction, identifying Cavin-4 as a novel muscle disease candidate caveolar protein. (
  • 2014.Rab5 is required in metastatic cancer cells for Caveolin-1-enhanced Rac1 activation, migration and invasion. (
  • Six Tissue Microarrays (TMAs) were performed and all the cases and biopsies were distributed into the following groups: a) only paraffin block available from primary and/or metastatic tumours (3 TMAs), b) paraffin block available from primary and/or metastatic tumours as well as from the corresponding Nude mice xenotransplant (2 TMAs), c) only paraffin block available from xenotransplanted Chs (1 TMA). (
  • The caveolin family is one of the best studied and the role of caveolin-1 is mainly determined by its ability to form signalosomes, i.e. not only to support the integrity of lipid rafts, but also, due to interaction with many residential signaling molecules, to coordinate and regulate signal transduction in the cell [ 1 ]. (
  • The role of lipid rafts was assessed by cholesterol depletion and caveolin co-localization.ResultsC. (
  • Acylated GFP chimeras did not cofractionate with low-density caveolin-rich lipid rafts made with Triton X-100 or detergent-free methods, and did not cofractionate with low-density caveolin-rich lipid rafts prepared with Triton X-100 or detergent-free methods. (
  • Tamoxifen in Duchenne muscular dystrophy (TAMDMD): study protocol for a multicenter, randomized, placebo-controlled, double-blind phase 3 trial. (
  • Penetration into the cell is through a caveolin vesicle. (
  • Caveolin-1 is a multi-functional protein required for caveolae formation and transendothelial transport and thus plays a critical role in lung microvascular endothelial cell barrier regulation and drug delivery. (
  • The focus of his current work is to understand how caveolin-regulated endothelial cell signaling mechanisms regulate caveolae formation and trafficking dynamics, cell-cell communication, PMN adhesion, and endothelial and epithelial cell proliferation and differentiation as critical components of lung vascular and airway development, permeability regulation, inflammatory injury, and repair/remodeling. (
  • These results show that, although N-terminal acylation will bring GFP to cholesterol and sphingolipid-enriched membranes, protein-protein interactions are required to localize a given protein to detergent-resistant membranes or caveolin-rich membranes. (
  • Overexpression of caveolin-3 leads to the development of cardiomyopathy, resulting in degeneration of cardiac tissue and manifesting pathologies due to the associated degeneration. (
  • Structural data indicated that cavins form hetero-trimers (mainly cavin1/2 or cavin1/3) and assemble as a layered protein coat with caveolins 8 - 10 . (
  • Rabbit polyclonal anti-caveolin-1, which immunoreacted with human being, mouse and rat caveolin-1, was bought from BD Biosciences Pharmingen (Franklin Lakes, NJ, USA). (
  • Caveolin-3 is concentrated in the caveolae of myocytes, and modulates numerous metabolic processes including: nitric oxide synthesis, cholesterol metabolism, and cardiac myocytes contraction. (
  • Caveolin-3 associates with the cardiac sodium-calcium exchanger (NCX) in caveolae of cardiac myocytes. (
  • Interactions between caveolin-3 and cardiac NCX influence NCX-regulation of cellular signaling factors and excitation of cardiac myocytes. (
  • Caveolin-3 influences the opening of L-Type calcium channels (LTCC) which play a role in cardiac myocyte contraction. (
  • Disruption of caveolin-3 disturbs the structure of cardiac caveolae and blocks atrial natriuretic peptide (ANP) expression, a cardiac-related hormone involved in many functions including maintaining cellular homeostasis. (
  • Normal caveolin-3 expression under conditions of stress increases cardiac cellular levels of ANP, maintaining cardiac homeostasis. (
  • In mice, cardiac preconditioning from isoflurane involved increased caveolin levels in mitochondria and their associated improved respiratory function. (
  • 7-KC-reduced doxorubicin deposition could possibly be reversed by inhibitors of phosphatidylinositol 3-kinase, Akt, and mammalian focus on of rapamycin (mTOR). (
  • During differentiation, L6 rat myoblasts demonstrated a fusion-related increase in activity associated with the 25/26-kDa, fully processed, active form of cathepsin B. Immunocytochemical studies demonstrated a redistribution of lysosomal cathepsin B protein toward the membrane of fusing myoblasts, and a colocalization of cathepsin B with caveolin-3, the muscle-specific structural protein of membrane caveolae. (
  • Our study is the first to show that the proteolytic activity of cathepsin B and its co-expression with caveolin-1 contributes to the aggressiveness of IBC. (
  • Downregulation of caveolin-1, the structural protein of caveolae, reduces the cell surface association of cathepsin B and decreases degradation of type IV collagen and invasion by the colon cancer cells, consistent with a functional role for caveolae-associated cathepsin B in invasion [ 12 ]. (
  • Our data shows that the structure of the mobile proteome is inspired by integrin appearance patterns and reveals a solid functional romantic relationship between 3 integrin and cathepsin B. raised angiogenesis (8, 9). (
  • This was accompanied by a marked decrease in caveolin-3 mRNA and protein levels compared with non-TG control mice. (
  • The latest work involved injecting a harmless adeno-associated viral vector carrying synapsin-Caveolin-1 cDNA (AAV9-SynCav1) into the spinal cords of familial ALS mice to see if it would delay disease progression and preserve physical strength and mobility. (
  • Journal of comparative pathology, 147, 2-3. (
  • These organelles are characterized by the presence of the caveolin-1 (Cav-1) protein that plays an important role in the regulation of several signal transduction pathways. (
  • 2014. Nobiletin suppresses cell viability through AKT pathways in PC-3 and DU-145 prostate cancer cells. (
  • Knockout of caveolin-3 genes are sufficient to induce these manifestations. (
  • Caveolin isoform expression during differentiation of C6 glioma cells. (
  • The amount of KATP activation during times of biological stress influences the amount of cellular damage that will occur, thus regulation of caveolin-3 expression during these times influences the amount of cellular damage. (
  • Alterations in caveolin-3 expression have been implicated in the altered expression and regulation of numerous signaling molecules involved in cardiomyopathies. (
  • Regulation of the functional pool of acetylcholine receptors via the interaction with caveolin-1-positive membrane microdomains. (
  • Background Human cancers show genomic instability and heightened drug sensitivity due to underlying problems in DNA restoration or cell cycle regulation [1-3]. (
  • Role of Caveolin-1 in Cancer and Therapeutic Implications, Cutting Edge Therapies for Cancer in the 21st Century (2014) 1: 3. (
  • Cyclopamine The role of caveolin-3 in heart muscle disease is controversial. (
  • Role of Caveolin-1 in carbon nanotube -induced stem-like cells and tumorigenesis. (
  • Caveolin-1 and miR-7-5p play an important role in the process of low shear stress inhibiting endothelial mitophagy. (
  • In response to excess steroid in the can be administered scherer PE, Lisanti MP: Role of caveolin-1 performance-enhancing drug or medication. (
  • 3 In addition, ceryl alcohol as well as some essential oils and fatty acids may play an active role in the pharmacological properties of horny goat weed. (
  • A pattern of MT 3 mRNA expres sion similar to that to the parental UROtsa cells was uncovered following treatment in the Cd 2 and As three trans formed cell lines with five AZC and MS 275. (
  • The sole exception staying the expression of MT 3 mRNA was many fold increased following MS 275 treatment while in the Cd two and As three transformed cell lines in contrast on the parental UROtsa cells. (
  • The initial indica tion the integrity with the MT three promoter may very well be distinctive among the parent and transformed UROtsa cells, was that MT 3 mRNA expression could possibly be additional induced by Zn two during the transformed cell lines following treatment method with MS 275, but was not induced by an identical therapy within the parental UROtsa cell line. (
  • After cleaning 3 x for 5 min the precise peroxidase-conjugated supplementary antibody was added and incubation was completed for 1 h at RT. (
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Galectin 3 (GAL3) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Adenylate Kinase 3 (AK3) in serum, plasma, cerebrospinal fluid and other biological fluids. (
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Adenylate Kinase 3 (AK3) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (
  • Because of this, the range of weakness and the progression of the condition of people with caveolin 3 deficiency are not well known. (
  • In addition, it is estimated that 25% of all patients diagnosed with lung cancer worldwide are not attributable to smoking [ 3 , 4 ]. (
  • Serum 7-KC concentrations in lung and rectal cancers sufferers had been 2- KRN 633 to 3-flip greater than those in healthful individuals [10]. (
  • Using transmission electron microscopy and single particle analysis methods, it has been shown that nine Caveolin-3 monomers assemble to form a complex that is toroidal in shape, ~16.5 nm in diameter and ~5.5 nm in height. (
  • Although there was heterogeneity in immunohistochemistry results of the different material analyzed, S100, SOX-9, Caveolin and Survivin were more expressed. (
  • Human Galectin-3 is a 26 kDa protein that can be nuclear, cytoplasmic, or secreted (3, 4). (
  • These substances localize to multiple sperm domains like the acrosomal cover (IZUMO caveolin 2 and flotillin 2) equatorial section (GM3) cytoplasmic droplet (TEX101) midpiece (basigin facilitated blood sugar transporter 3 and flotillin 2) and primary piece (facilitated blood sugar transporter 3). (
  • Clathrin- and Caveolin-Independent Entry of Human Papillomavirus Type 16-Involvement of Tetraspanin-Enriched Microdomains (TEMs). (
  • Epithelial permeability was assessed by measuring flux of a 3 kDa dextran probe. (
  • Projects are ongoing to understand the effects of mechanical forces on epithelial and endothelial signaling mediated by p120 catenin, caveolin-1, ICAM as well as associated downstream effects on neutrophil recruitment and innate immune response. (
  • To date, the analysis of caveolin-1 expression has been carried out in a wide range of tumors and cell lines of various origins. (
  • Expression of Caveolin-3 in the Muscle Cell and Tissue. (
  • Cell Mol Neurobiol 32(3): 409-421. (
  • Modulation of 14-3-3 protein association may provide of a mechanism of regulating cell-cell adhesion. (
  • If your other family pressure shows effectively adjust macrophages for persons with subunits sites similar as identification, have to activate expression within the factor death that this is primarily cell-type with the cellular 3-hydroxyacyl-CoA dichain terminal, activated by the Marrakech Treaty and the viral activity of Understanding. (
  • Extracellular Galectin-3 has been shown to form high-order oligomers that promote the crosslinking of cell surface oligosacchraides as well as integrin-dependent cell adhesion and apoptosis (8-11). (
  • Galectin-3 contributes to the innate immune response against Candida albicans and Streptococcus pneumoniae , and it can facilitate acute inflammatory responses via neutrophil activation and opsonization, macrophage recruitment, and mast cell activation (12-14). (
  • Description: A sandwich quantitative ELISA assay kit for detection of Mouse Galectin 3 (GAL3) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. (
  • These scientific studies demonstrate that there is a fundamental distinction in the accessibility of MREs to MTF 1 binding inside the MT 3 promoter among the parental UROtsa cells and also the Cd two and As three trans formed cell lines. (