Caveolin 2: Caveolin 2 is a binding partner of CAVEOLIN 1. It undergoes tyrosine phosphorylation by C-SRC PROTEIN PP60 and plays a regulatory role in CAVEOLAE formation.Caveolae: Endocytic/exocytic CELL MEMBRANE STRUCTURES rich in glycosphingolipids, cholesterol, and lipid-anchored membrane proteins that function in ENDOCYTOSIS (potocytosis), transcytosis, and SIGNAL TRANSDUCTION. Caveolae assume various shapes from open pits to closed vesicles. Caveolar coats are composed of CAVEOLINS.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Membrane Microdomains: Detergent-insoluble CELL MEMBRANE components. They are enriched in SPHINGOLIPIDS and CHOLESTEROL and clustered with glycosyl-phosphatidylinositol (GPI)-anchored proteins.Filipin: A complex of polyene antibiotics obtained from Streptomyces filipinensis. Filipin III alters membrane function by interfering with membrane sterols, inhibits mitochondrial respiration, and is proposed as an antifungal agent. Filipins I, II, and IV are less important.Detergents: Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.Cell Compartmentation: A partitioning within cells due to the selectively permeable membranes which enclose each of the separate parts, e.g., mitochondria, lysosomes, etc.Cholesterol: The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.beta-Cyclodextrins: Cyclic GLUCANS consisting of seven (7) glucopyranose units linked by 1,4-glycosidic bonds.Clathrin: The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. Each molecule of clathrin consists of three light chains (CLATHRIN LIGHT CHAINS) and three heavy chains (CLATHRIN HEAVY CHAINS) that form a structure called a triskelion. Clathrin also interacts with cytoskeletal proteins.Endocytosis: Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Octoxynol: Nonionic surfactant mixtures varying in the number of repeating ethoxy (oxy-1,2-ethanediyl) groups. They are used as detergents, emulsifiers, wetting agents, defoaming agents, etc. Octoxynol-9, the compound with 9 repeating ethoxy groups, is a spermatocide.Cyclodextrins: A homologous group of cyclic GLUCANS consisting of alpha-1,4 bound glucose units obtained by the action of cyclodextrin glucanotransferase on starch or similar substrates. The enzyme is produced by certain species of Bacillus. Cyclodextrins form inclusion complexes with a wide variety of substances.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Dogs: The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)MyoglobinuriaCell Line: Established cell cultures that have the potential to propagate indefinitely.Nitric Oxide Synthase Type III: A CALCIUM-dependent, constitutively-expressed form of nitric oxide synthase found primarily in ENDOTHELIAL CELLS.Microscopy, Immunoelectron: Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays.Cell Fractionation: Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.3T3 Cells: Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.Golgi Apparatus: A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Protein Transport: The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.Annexin A6: Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.Neoplasms, Adipose Tissue: Neoplasms composed of fatty tissue or connective tissue made up of fat cells in a meshwork of areolar tissue. The concept does not refer to neoplasms located in adipose tissue.Dynamin II: A subtype of dynamin found ubiquitously expressed in a variety of tissues.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Adipocytes: Cells in the body that store FATS, usually in the form of TRIGLYCERIDES. WHITE ADIPOCYTES are the predominant type and found mostly in the abdominal cavity and subcutaneous tissue. BROWN ADIPOCYTES are thermogenic cells that can be found in newborns of some species and hibernating mammals.Glycosylphosphatidylinositols: Compounds containing carbohydrate or glycosyl groups linked to phosphatidylinositols. They anchor GPI-LINKED PROTEINS or polysaccharides to cell membranes.Cytoplasmic Vesicles: Membrane-limited structures derived from the plasma membrane or various intracellular membranes which function in storage, transport or metabolism.Sequence Tagged Sites: Short tracts of DNA sequence that are used as landmarks in GENOME mapping. In most instances, 200 to 500 base pairs of sequence define a Sequence Tagged Site (STS) that is operationally unique in the human genome (i.e., can be specifically detected by the polymerase chain reaction in the presence of all other genomic sequences). The overwhelming advantage of STSs over mapping landmarks defined in other ways is that the means of testing for the presence of a particular STS can be completely described as information in a database.Dynamins: A family of high molecular weight GTP phosphohydrolases that play a direct role in vesicle transport. They associate with microtubule bundles (MICROTUBULES) and are believed to produce mechanical force via a process linked to GTP hydrolysis. This enzyme was formerly listed as EC 3.6.1.50.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Microscopy, Confocal: A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.Nitric Oxide Synthase: An NADPH-dependent enzyme that catalyzes the conversion of L-ARGININE and OXYGEN to produce CITRULLINE and NITRIC OXIDE.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Adenomyosis: The extension of endometrial tissue (ENDOMETRIUM) into the MYOMETRIUM. It usually occurs in women in their reproductive years and may result in a diffusely enlarged uterus with ectopic and benign endometrial glands and stroma.Solubility: The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Endosomes: Cytoplasmic vesicles formed when COATED VESICLES shed their CLATHRIN coat. Endosomes internalize macromolecules bound by receptors on the cell surface.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Centrifugation, Zonal: Centrifugation using a rotating chamber of large capacity in which to separate cell organelles by density-gradient centrifugation. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Muscle Proteins: The protein constituents of muscle, the major ones being ACTINS and MYOSINS. More than a dozen accessory proteins exist including TROPONIN; TROPOMYOSIN; and DYSTROPHIN.Amino Acids, Aromatic: Amino acids containing an aromatic side chain.Glucose Transporter Type 4: A glucose transport protein found in mature MUSCLE CELLS and ADIPOCYTES. It promotes transport of glucose from the BLOOD into target TISSUES. The inactive form of the protein is localized in CYTOPLASMIC VESICLES. In response to INSULIN, it is translocated to the PLASMA MEMBRANE where it facilitates glucose uptake.Microscopy, Fluorescence: Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.Proto-Oncogene Proteins c-fyn: Src-family kinases that associate with T-CELL ANTIGEN RECEPTOR and phosphorylate a wide variety of intracellular signaling molecules.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Precipitin Tests: Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.Membrane Lipids: Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.Androstenes: Unsaturated derivatives of the steroid androstane containing at least one double bond at any site in any of the rings.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.src-Family Kinases: A PROTEIN-TYROSINE KINASE family that was originally identified by homology to the Rous sarcoma virus ONCOGENE PROTEIN PP60(V-SRC). They interact with a variety of cell-surface receptors and participate in intracellular signal transduction pathways. Oncogenic forms of src-family kinases can occur through altered regulation or expression of the endogenous protein and by virally encoded src (v-src) genes.Spodoptera: A genus of owlet moths of the family Noctuidae. These insects are used in molecular biology studies during all stages of their life cycle.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Cholesterol Oxidase: An enzyme that catalyzes the oxidation of cholesterol in the presence of molecular oxygen to 4-cholesten-3-one and hydrogen peroxide. The enzyme is not specific for cholesterol, but will also oxidize other 3-hydroxysteroids. EC 1.1.3.6.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Thioredoxin Reductase 1: A subtype of thioredoxin reductase found primarily in the CYTOSOL.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.Muscular Dystrophies: A heterogeneous group of inherited MYOPATHIES, characterized by wasting and weakness of the SKELETAL MUSCLE. They are categorized by the sites of MUSCLE WEAKNESS; AGE OF ONSET; and INHERITANCE PATTERNS.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Fluorescent Antibody Technique, Indirect: A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)Recombinant Proteins: Proteins prepared by recombinant DNA technology.Subcellular Fractions: Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)Nitric Oxide: A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.Coatomer Protein: A 700-kDa cytosolic protein complex consisting of seven equimolar subunits (alpha, beta, beta', gamma, delta, epsilon and zeta). COATOMER PROTEIN and ADP-RIBOSYLATION FACTOR 1 are principle components of COAT PROTEIN COMPLEX I and are involved in vesicle transport between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.Antigens, CD55: GPI-linked membrane proteins broadly distributed among hematopoietic and non-hematopoietic cells. CD55 prevents the assembly of C3 CONVERTASE or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex.Receptor, Nerve Growth Factor: A low affinity receptor that binds NERVE GROWTH FACTOR; BRAIN-DERIVED NEUROTROPHIC FACTOR; NEUROTROPHIN 3; and neurotrophin 4.Monosaccharide Transport Proteins: A large group of membrane transport proteins that shuttle MONOSACCHARIDES across CELL MEMBRANES.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Immunoblotting: Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.Endothelium, Vascular: Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.Clathrin-Coated Vesicles: Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles is covered with a lattice-like network of the protein CLATHRIN. Shortly after formation, however, the clathrin coat is removed and the vesicles are referred to as ENDOSOMES.GTP Phosphohydrolases: Enzymes that hydrolyze GTP to GDP. EC 3.6.1.-.COS Cells: CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)Intracellular Membranes: Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.G(M1) Ganglioside: A specific monosialoganglioside that accumulates abnormally within the nervous system due to a deficiency of GM1-b-galactosidase, resulting in GM1 gangliosidosis.Glycosphingolipids: Lipids containing at least one monosaccharide residue and either a sphingoid or a ceramide (CERAMIDES). They are subdivided into NEUTRAL GLYCOSPHINGOLIPIDS comprising monoglycosyl- and oligoglycosylsphingoids and monoglycosyl- and oligoglycosylceramides; and ACIDIC GLYCOSPHINGOLIPIDS which comprises sialosylglycosylsphingolipids (GANGLIOSIDES); SULFOGLYCOSPHINGOLIPIDS (formerly known as sulfatides), glycuronoglycosphingolipids, and phospho- and phosphonoglycosphingolipids. (From IUPAC's webpage)Tissue Distribution: Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Heterotrimeric GTP-Binding Proteins: GTP-BINDING PROTEINS that contain three non-identical subunits. They are found associated with members of the seven transmembrane domain superfamily of G-PROTEIN-COUPLED RECEPTORS. Upon activation the GTP-BINDING PROTEIN ALPHA SUBUNIT of the complex dissociates leaving a dimer of a GTP-BINDING PROTEIN BETA SUBUNIT bound to a GTP-BINDING PROTEIN GAMMA SUBUNIT.Brefeldin A: A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.Histidine: An essential amino acid that is required for the production of HISTAMINE.GTP-Binding Protein alpha Subunits, Gi-Go: A family of heterotrimeric GTP-binding protein alpha subunits that were originally identified by their ability to inhibit ADENYLYL CYCLASES. Members of this family can couple to beta and gamma G-protein subunits that activate POTASSIUM CHANNELS. The Gi-Go part of the name is also spelled Gi/Go.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.CHO Cells: CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.Virus Internalization: The entering of cells by viruses following VIRUS ATTACHMENT. This is achieved by ENDOCYTOSIS, by direct MEMBRANE FUSION of the viral membrane with the CELL MEMBRANE, or by translocation of the whole virus across the cell membrane.

NO synthase II in mouse skeletal muscle is associated with caveolin 3. (1/243)

The inducible-type NO synthase (NOS II; iNOS) is constitutively expressed in slow-twitch skeletal muscle fibres of guinea-pigs [Gath, Closs, Godtel-Armbrust, Schmitt, Nakane, Wessler and Forstermann (1996) FASEB J. 10, 1614-1620]. Here we studied the expression of NOS II in skeletal muscle of wild-type and NOS II-deficient mice and investigated the molecular basis for the membrane association of this NOS in muscle. A basal expression of NOS II mRNA and protein was detected in skeletal muscle from untreated wild-type mice; expression increased when mice were treated with bacterial lipopolysaccharide (LPS). No NOS II was found in any tissue of untreated or LPS-treated NOS II-deficient mice. Immunoprecipitation experiments were performed with homogenates of gastrocnemius muscle from untreated or LPS-treated wild-type mice. A NOS II-specific antibody precipitated caveolin 3 in all homogenates investigated, the effect being most pronounced in skeletal muscle from LPS-treated animals. Conversely, an antibody against caveolin 3 co-precipitated NOS II in muscle homogenates. Similarly, a weak co-precipitation of NOS II and caveolin 3 was seen in homogenates of untreated murine C2C12 myotubes; co-precipitation was markedly enhanced in cells stimulated with LPS/interferon gamma. The association of NOS II with caveolin 3 might have implications for the regulation of contraction of, and/or glucose uptake by, slow-twitch muscle fibres.  (+info)

Neuregulin signaling in the heart. Dynamic targeting of erbB4 to caveolar microdomains in cardiac myocytes. (2/243)

Two of the neuregulins (NRG1 and NRG2) and their receptors (erbB2 and erbB4) are essential for normal cardiac development and can mediate hypertrophic growth and enhance survival of embryonic, postnatal, and adult rat ventricular myocytes. The expression of erbB4, the predominant NRG receptor in postnatal rat ventricular muscle, declines after midembryogenesis, and its expression is limited to cardiac myocytes. A full-length erbB4 rat cDNA isolated from neonatal ventricular muscle was found to be highly homologous to human erbB4 and contained a caveolin binding motif within the cytoplasmic kinase domain. Using the complementary techniques of detergent-free density-gradient ultracentrifugation of myocyte lysates and coimmunoprecipitation of erbB4 and caveolin-3, the caveolin isoform expressed in cardiac myocytes, erbB4 could be localized (using both approaches) to caveolar microdomains. Moreover, addition of a soluble NRG1, recombinant human glial growth factor 2, resulted in rapid (2-minute) translocation of erbB4 out of caveolar microdomain in cardiac myocytes. Thus, erbB4 is dynamically targeted to caveolar microdomains within cardiac myocytes. Its rapid translocation after NRG1 binding may contribute to receptor desensitization in the continuous presence of ligand.  (+info)

Molecular characterization of caveolin association with the Golgi complex: identification of a cis-Golgi targeting domain in the caveolin molecule. (3/243)

Caveolins are integral membrane proteins which are a major component of caveolae. In addition, caveolins have been proposed to cycle between intracellular compartments and the cell surface but the exact trafficking route and targeting information in the caveolin molecule have not been defined. We show that antibodies against the caveolin scaffolding domain or against the COOH terminus of caveolin-1 show a striking specificity for the Golgi pool of caveolin and do not recognize surface caveolin by immunofluorescence. To analyze the Golgi targeting of caveolin in more detail, caveolin mutants were expressed in fibroblasts. Specific mutants lacking the NH2 terminus were targeted to the cis Golgi but were not detectable in surface caveolae. Moreover, a 32-amino acid segment of the putative COOH-terminal cytoplasmic domain of caveolin-3 was targeted specifically and exclusively to the Golgi complex and could target a soluble heterologous protein, green fluorescent protein, to this compartment. Palmitoylation-deficient COOH-terminal mutants showed negligible association with the Golgi complex. This study defines unique Golgi targeting information in the caveolin molecule and identifies the cis Golgi complex as an intermediate compartment on the caveolin cycling pathway.  (+info)

Localization of the human caveolin-3 gene to the D3S18/D3S4163/D3S4539 locus (3p25), in close proximity to the human oxytocin receptor gene. Identification of the caveolin-3 gene as a candidate for deletion in 3p-syndrome. (4/243)

Caveolin-3, a muscle-specific caveolin-related protein, is the principal structural protein of caveolae membrane domains in striated muscle cell types (cardiac and skeletal). Recently, we identified an autosomal dominant form of limb girdle muscular dystrophy in humans that is due to mutations within exon 2 of the caveolin-3 gene (3p25). However, the detailed location of the human caveolin-3 gene and its position with regard to neighboring genes remains unknown. Here, we have isolated three independent BAC clones containing the human caveolin-3 gene. Using a PCR-based approach, we determined that these clones contain both exons 1 and 2 of the human caveolin-3 gene. In addition, we performed microsatellite marker analysis of these BAC clones, using a panel of 13 markers that are known to map within the 3p25 region. Our results indicate that these BAC clones contain the following three markers: D3S18, SHGC-1079 (also known as D3S4163) and D3S4539. Interestingly, D3S18 is a marker for two known human diseases, von Hippel-Lindau disease and 3p-syndrome. As D3S4163 and D3S4539 are known to map in the vicinity of the 3' end of the human oxytocin receptor gene, we determined if these caveolin-3 positive BACs also contain the oxytocin receptor gene. We show that (i) these BACs contain all four exons of the oxytocin receptor gene and (ii) that the genes encoding caveolin-3 and the oxytocin receptor are located approximately 7-10 kb apart and in the opposite orientation. As 3p-syndrome is characterized by cardiac septal defects and caveolin-3 is expressed primarily in the heart and skeletal muscle, caveolin-3 is a candidate gene that may be deleted in 3p-syndrome.  (+info)

Caveolin-3 upregulation activates beta-secretase-mediated cleavage of the amyloid precursor protein in Alzheimer's disease. (5/243)

Here, we investigate the involvement of caveolins in the pathophysiology of Alzheimer's disease (AD). We show dramatic upregulation of caveolin-3 immunoreactivity in astroglial cells surrounding senile plaques in brain tissue sections from authentic AD patients and an established transgenic mouse model of AD. In addition, we find that caveolin-3 physically interacts and biochemically colocalizes with amyloid precursor protein (APP) both in vivo and in vitro. Interestingly, recombinant overexpression of caveolin-3 in cultured cells stimulated beta-secretase-mediated processing of APP. Immunoreactivities of APP and presenilins were concomitantly increased in caveolin-3-positive astrocytes. Because the presenilins also form a physical complex with caveolin-3, caveolin-3 may provide a common platform for APP and the presenilins to associate in astrocytes. In AD, augmented expression of caveolin-3 and presenilins in reactive astrocytes may alter APP processing, leading to the overproduction of its toxic amyloid metabolites.  (+info)

Phenotypic behavior of caveolin-3 mutations that cause autosomal dominant limb girdle muscular dystrophy (LGMD-1C). Retention of LGMD-1C caveolin-3 mutants within the golgi complex. (6/243)

Caveolin-3, a muscle-specific caveolin-related protein, is the principal structural protein of caveolae membrane domains in striated muscle cell types (cardiac and skeletal). Autosomal dominant limb girdle muscular dystrophy (LGMD-1C) in humans is due to mutations within the caveolin-3 gene: (i) a 9-base pair microdeletion that removes three amino acids within the caveolin scaffolding domain (DeltaTFT) or (ii) a missense mutation within the membrane spanning domain (P --> L). The molecular mechanisms by which these two mutations cause muscular dystrophy remain unknown. Here, we investigate the phenotypic behavior of these caveolin-3 mutations using heterologous expression. Wild type caveolin-3 or caveolin-3 mutants were transiently expressed in NIH 3T3 cells. LGMD-1C mutants of caveolin-3 (DeltaTFT or P --> L) were primarily retained at the level of a perinuclear compartment that we identified as the Golgi complex in double-labeling experiments, while wild type caveolin-3 was efficiently targeted to the plasma membrane. In accordance with these observations, caveolin-3 mutants formed oligomers of a much larger size than wild type caveolin-3 and were excluded from caveolae-enriched membrane fractions as seen by sucrose density gradient centrifugation. In addition, these caveolin-3 mutants were expressed at significantly lower levels and had a dramatically shortened half-life of approximately 45-60 min. However, caveolin-3 mutants were palmitoylated to the same extent as wild type caveolin-3, indicating that targeting to the plasma membrane is not required for palmitoylation of caveolin-3. In conclusion, we show that LGMD-1C mutations lead to formation of unstable high molecular mass aggregates of caveolin-3 that are retained within the Golgi complex and are not targeted to the plasma membrane. Consistent with its autosomal dominant form of genetic transmission, we demonstrate that LGMD-1C mutants of caveolin-3 behave in a dominant-negative fashion, causing the retention of wild type caveolin-3 at the level of the Golgi. These data provide a molecular explanation for why caveolin-3 levels are down-regulated in patients with this form of limb girdle muscular dystrophy (LGMD-1C).  (+info)

The limb-girdle muscular dystrophies-multiple genes, multiple mechanisms. (7/243)

In the field of muscular dystrophy, advances in understanding the molecular basis of the various disorders in this group have been rapidly translated into readily applicable diagnostic tests, allowing the provision of more accurate prognostic and genetic counselling. The limb-girdle muscular dystrophies (LGMD) have recently undergone a major reclassification according to their genetic basis. Currently 13 different types can be recognized. Amongst this group, increasing diversity of the mechanisms involved in producing a muscular dystrophy phenotype is emerging. Recent insights into the involvement of the dystrophin glycoprotein complex in muscular dystrophy suggests that its members may play distinct or even multiple roles in the maintenance of muscle fibre integrity. In other forms of LGMD, proteins have been implicated which may be important in intracellular signalling, vesicle trafficking or the control of transcription. As these various mechanisms are more fully elucidated, further insights will be gained into the pathophysiology of muscular dystrophy. At a practical level, despite the marked heterogeneity of this group real progress can at last be made in determining a precise diagnosis.  (+info)

Codistribution of NOS and caveolin throughout peripheral vasculature and skeletal muscle of hamsters. (8/243)

In isolated cell systems, nitric oxide synthase (NOS) activity is regulated by caveolin (CAV), a resident caveolae coat protein. Because little is known of this interaction in vivo, we tested whether NOS and caveolin are distributed together in the intact organism. Using immunohistochemistry, we investigated the localization of constitutive neuronal (nNOS) and endothelial (eNOS) enzyme isoforms along with caveolin-1 (CAV-1) and caveolin-3 (CAV-3) throughout the systemic vasculature and peripheral tissues of the hamster. The carotid artery, abdominal aorta, vena cava, femoral artery and vein, feed artery and collecting vein of the cheek pouch retractor muscle, capillaries and muscle fibers of retractor and cremaster muscles, and arterioles and venules of the cheek pouch were studied. In endothelial cells, eNOS and CAV-1 were present throughout the vasculature, whereas nNOS and CAV-3 were absent except in capillaries, which reacted for nNOS. In smooth muscle cells, nNOS and CAV-1 were also expressed systemically, whereas eNOS was absent; CAV-3 was present in the arterial but not the venous vasculature. Both nNOS and CAV-3 were located at the sarcolemma of skeletal muscle fibers, which were devoid of eNOS and CAV-1. These immunolabeling patterns suggest functional interactions between eNOS and CAV-1 throughout the endothelium, regional differences in the modulation of nNOS by caveolin isoforms in vascular smooth muscle, and modulation of nNOS by CAV-3 in skeletal muscle.  (+info)

This issue contains an important contribution from the team of Douglas Lauffenburger, Chair of the Editorial Board, at MIT, USA, in which they demonstrate the ability to characterise quantitative data on phenotypic behaviour of individual endothelial cells in response to angiogenesis signalling and relate it to overall population behaviour. They show that the behaviour of microvascular endothelial cells in a single population is heterogeneous and cannot be assumed to be the same for all cells in a given condition.. Endothelial cell phenotypic behaviors cluster into dynamic state transition programs modulated by angiogenic and angiostatic ...
Title: Coordinated Expression of Pax-5 and FAK1 in Metastasis. VOLUME: 11 ISSUE: 7. Author(s):Nicolas Crapoulet, Pierre OBrien, Rodney J. Ouellette and Gilles A. Robichaud. Affiliation:Universite de Moncton, Moncton, NB, Canada, E1A 3E9.. Keywords:Cancer, cell signaling, FAK1, focal adhesion, metastasis, Pax-5, B lymphopoiesis, cell differentiation, homeostasis, leukemia. Abstract: The Pax-5 gene encodes a B-cell-specific activator protein (BSAP) that plays a key role in B lymphocyte differentiation and embryogenesis. The deregulation of this transcription factor is also linked to B cell malignancies and recently to other cancers. More specifically, the downstream effects of Pax-5 promote cell-cell interactions and mediate the activation of adhesion genes which result in an epithelial phenotypic behavior of human carcinoma cells. To gain a better understanding of Pax-5-mediated gene regulation, we studied available gene expression data in depth and identified several Pax-5 downstream targets. ...
The proteoglycan-dystrophin complex in genetic cardiomyopathies--lessons from three siblings with limb-girdle muscular dystrophy-2I (LGMD-2I).:
www.MOLUNA.de Caveolins in Cancer Pathogenesis, Prevention and Therapy [4196990] - Caveolins play an important role in the pathogenesis of multiple cancers. This volume focuses mainly on the importance of Caveolin-1 in breast, prostate, lung, skin, colon, pancreatic and brain cancers. It also studies the role of Caveolin-3 in breast cancer.Caveolins are important structural proteins of Caveolae, small invaginations of the
To increase tolerance to abiotic stresses in breeding programmes, typically families and collections of genotypes are evaluated in series of trials (environments) representing different levels of stress. The statistical analysis of the data from such trials concentrates on modelling the phenotypic behaviour of the genotypes across the set of environments. This phenotypic behaviour can be modelled in the form of genotype-specific linear and non-linear response curves in relation to environmental characterizations. Non-parallelism of the response curves indicates genotype × environment interaction. Identification of the genetic basis of the parameters determining the response curves will help in the development of breeding programmes for improving abiotic stress tolerance and understanding genotype × environment interaction. In this paper we present two strategies for locating quantitative trait loci for response-curve parameters and estimation of their allele effects. The procedures are ...
At the European Molecular Biology Laboratory (EMBL) in Heidelberg, Professor Parton was mentored by Gareth Griffiths, where he honed his skills in electron microscopy while collaborating with other groups throughout the institute including Jean Gruenberg and Marino Zerial. He has maintained these collaborations to this day, despite the groups now being spread around the globe.. Collaboration has been central to his career. In a collaborative project with the laboratory of Kai Simons, he observed that the newly-discovered protein, VIP21 (later renamed caveolin-1), was an abundant marker protein of caveolae. Also at the EMBL Parton and Michael Way discovered a second member of the caveolin family, M-caveolin, now termed caveolin-3.. At The University of Queensland he collaborated with Professor John Hancock. Together they showed that caveolin mutants can act as dominant negative inhibitory mutants and that one of the mutants was a highly potent inhibitor of Ras signalling. Inhibition was specific ...
This download caveolins was been, and received on the treatment by Sir Henry Irving in 1875. Harold was in 1876, The Cup in 1881( at the Lyceum), The Promise of May( at the Globe) in 1882, Becket in 1884, with The Foresters in 1892. The download is one from which I give, not right of secure single-player of the purchase and of of rendering for the analysis.
Biochemical network reconstructions have become popular tools in systems biology. Metabolicnetwork reconstructions are biochemically, genetically, and genomically (BiGG) structured databases of biochemical reactions and metabolites. They contain information such as exact reaction stoichiometry, reaction reversibility, and the relationships between genes, proteins, and reactions. Network reconstructions have been used extensively to study the phenotypic behavior of wild-type and mutant stains under a variety of conditions, linking genotypes with phenotypes. Such phenotypic simulations have allowed for the prediction of growth after genetic manipulations, prediction of growth phenotypes after adaptive evolution, and prediction of essential genes. Additionally, because network reconstructions are organism specific, they can be used to understand differences between organisms of species in a functional context.There are different types of reconstructions representing various types of biological networks
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This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites. [provided by RefSeq, Jul 2008 ...
Expression of caveolins in RMS tumours. Double immunostain showing that in skeletal muscle Cav-1 and Cav-3 mark satellite cells and the plasmalemma of myofibres
A pacing system delivers cardiac protection pacing to protect the heart from injuries associated with ischemic events. The pacing system detects an ischemic event and, in response, initiates one or more cardiac protection pacing sequences each including alternative pacing and non-pacing periods. In one embodiment, the pacing system initiates cardiac protection pacing sequences including at least one postconditioning sequence to protect the heart from a detected ischemic event and a plurality prophylactic preconditioning sequences to protect the heart from probable future ischemic events.
A pacing system delivers cardiac protection pacing to protect the heart from injuries associated with ischemic events. The pacing system detects an ischemic event and, in response, initiates one or more cardiac protection pacing sequences each including alternative pacing and non-pacing periods. In one embodiment, the pacing system initiates a cardiac protection pacing sequence in response to the detection of the onset of an ischemic event, such that a pacing concurrent conditioning therapy is applied during the detected ischemic event.
Dysferlin兔多克隆抗体(ab15108)可与狗, 人样本反应并经WB, IHC, ICC/IF实验严格验证,被3篇文献引用并得到3个独立的用户反馈。所有产品均提供质保服务,中国75%以上现货。
The limb‐girdle muscular dystrophies (LGMDs) area group of genetically heterogeneous neuromuscular disorders caused by specific protein defects in muscle fibres and characterised by predominant weakness and wasting in proximal limb and axial muscles
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity).
Caveolin-2兔单克隆抗体[EPR2220(3)](ab124883)可与人样本反应并经WB, Flow Cyt实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
In this study, we examined cardiomyocyte hypertrophy by several methods. PE and ET increased leucine incorporation and cell area and changed the immunostaining pattern of phalloidin from a dense staining without agonists to fiberlike staining, indicating that myocyte hypertrophy did occur at the protein and structural levels. Moreover, βMHC expression was markedly augmented by PE and ET, suggesting the transformation of myosin. These results indicate that PE and ET induced pathologic hypertrophy in cardiomyocytes. Infection with Ad.Cav-3 prevented all of these changes induced by PE and ET. Thus, caveolin-3 might act as an inhibitor of myocyte hypertrophy. The effects of overexpression of wild-type caveolin-3 were not nonspecific, because infection with Ad.LacZ did not affect myocyte hypertrophy. Overexpression of caveolin-3 in Ad.Cav-3-infected cells was associated with the prevention of both agonist-induced hypertrophy and sarcomeric disorganization seen in Ad.LacZ-infected and control cells ...
Caveolins act as scaffold proteins in multiprotein complexes and have been implicated in signaling by G protein-coupled receptors. Studies using knock-out mice suggest that beta(3)-adrenoceptor (beta(3)-AR) signaling is dependent on caveolin-1; however, it is not known whether caveolin-1 is associated with the beta(3)-AR or solely with downstream signaling proteins. We have addressed this question by examining the impact of membrane rafts and caveolin-1 on the differential signaling of mouse beta(3a)- and beta(3b)-AR isoforms that diverge at the distal C terminus. Only the beta(3b)-AR promotes pertussis toxin (PTX)-sensitive cAMP accumulation. When cells expressing the beta(3a)-AR were treated with filipin III to disrupt membrane rafts or transfected with caveolin-1 siRNA, the cyclic AMP response to the beta(3)-AR agonist CL316243 became PTX-sensitive, suggesting G alpha(i/o) coupling. The beta(3a)-AR C terminus, S (P-384) under bar PLNR (P-389) under bar DG (Y-392) under bar EGARP (P-398) under ...
Coronary Artery disease is one of the leading causes of death in china, followed by cancer. According to US CDC (Centre for Disease Control), cardiovascular disease is also the leading cause of death of men and women in the United States. Factors including hereditary and family history, nutrition and lifestyle, hypertension, high lipids and cholesterol etc, will increase the risk for cardiovascular diseases. A persons unique genetic makeup will also impact their responses to drugs eg. dosage, side effects etc. Cardiac Protection Genetic Test is conducted a CAP and CLIA accredited genetic laboratory in California US.. ...
Buy our Caveolin-2 peptide. Ab4929 is a blocking peptide and has been validated in BL. Abcam provides free protocols, tips and expert support for WB and a 12…
Caveolin-3 is a protein that in humans is encoded by the CAV3 gene. Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites. This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), HyperCKemia, distal myopathy or rippling muscle disease (RMD). Other mutations in Caveolin causes Long QT Syndrome or familial hypertrophic cardiomyopathy, although the role of Cav3 in Long QT syndrome has recently been disputed. Caveolin ...
Introduction: Caveolins (Cav), the principal structural proteins of the caveolar domain, have been implicated in the development of cardiac hypertrophy, pulmonary hypertension (PH) and lung remodeling. Mice with homozygous deletion of the Cav-1 gene display cardiac hypertrophy and pulmonary abnormalities characterized by thickened alveolar septa, hypercellularity and PH. In vivo administration of a cell-permeable Cav-1 mimetic peptide was previously shown to prevent the development of monocrotaline-induced pulmonary artery medial hypertrophy, PH and right ventricular hypertrophy in rats. Whether administration of such a Cav-1 peptide could rescue the cardio-pulmonary defects observed in Cav-1 KO mice remains unknown.. Methods and Results: Three week-old wild-type and Cav-1 KO mice were randomly assigned to receive a daily intraperitoneal injection of either saline, penetratin alone (AP, 2.5 mg/kg/d) or a peptide consisting of the Cav-1 scaffolding domain coupled to penetratin (AP-Cav, 2.5 ...
Caveolin-1 (Cav-1) is a 21?kDa protein enriched in caveolae and continues to be implicated in oncogenic cell transformation CHC tumorigenesis and metastasis. response to chemotherapy and radiation and tumor growth. We found Cav-1 is definitely overexpressed in human being Personal computer cell lines mouse models and human being pancreatic tumors and is associated with worse tumor grade and clinical results. In Personal computer cell lines disruption/depletion of caveolae/Cav-1 reduces proliferation colony formation and invasion. Radiation and chemotherapy up-regulate Cav-1 manifestation while Cav-1 depletion induces both chemosensitization and radiosensitization through modified apoptotic and DNA restoration signaling. and and transgene ("KPC mouse") (Fig. 1B). Cav-1 manifestation was also tested on a cells microarray of 110 individuals with pancreatic malignancy and obtained semi-quantitatively for low versus high CHC manifestation. While Cav-1 is definitely virtually absent in pancreatic ...
TY - JOUR. T1 - Caveolin-2 is targeted to lipid droplets, a new "membrane domain" in the cell. AU - Fujimoto, Toyoshi. AU - Kogo, Hiroshi. AU - Ishiguro, Kimiko. AU - Tauchi, Kumi. AU - Nomura, Ryuji. PY - 2001/3/5. Y1 - 2001/3/5. N2 - Caveolin-1 and -2 constitute a framework of caveolae in nonmuscle cells. In the present study, we showed that caveolin-2, especially its β isoform, is targeted to the surface of lipid droplets (LD) by immunofluorescence and immunoelectron microscopy, and by subcellular fractionation. Brefeldin A treatment induced further accumulation of caveolin-2 along with caveolin-1 in LD. Analysis of mouse caveolin-2 deletion mutants revealed that the central hydrophobic domain (residues 87-119) and the NH2-terminal (residues 70-86) and COOH-terminal (residues 120-150) hydrophilic domains are all necessary for the localization in LD. The NH2- and COOH-terminal domains appeared to be related to membrane binding and exit from ER, respectively, implying that caveolin-2 is ...
where to get vector overexpressing caveolin-1? - posted in Molecular Cloning: Trying to overexpress caveolin-1 in human cancer cells...are there readymade products out there which already has cav-1 attached to a dependable promoter? If so, where should I look? Thanks in advance!
Proteins and other substances can cross the endothelial layer that lines a blood vessel via two routes. Caveolin-1 is essential for both, Siddiqui et al. show.. Researchers already knew that caveolin-1 was necessary for transcellular protein trafficking, in which macromolecules such as albumin enter an endothelial cell from the bloodstream and exit on the tissue side. Caveolae swallow these molecular travelers and bundle them into vesicles that wend through the cell. In an alternative pathway, known as the paracellular route, molecules slip between the cells of the endothelial layer, passing through the adherens junctions that fasten adjacent cells together. Previous work showed that adherens junctions become permeable in mice lacking caveolin-1, suggesting that the protein helps seal the junctions.. Siddiqui et al. dissected the molecular chain of events that connects caveolin-1 to adherens junction integrity. Loss of caveolin-1 activated the enzyme eNOS, which spawns nitric oxide (NO) that ...
The scaffolding protein CAV1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to…
Limb-girdle muscular dystrophy (LGMD) is a rare heterogeneous group of human diseases. Besides the characteristic presentation of weakness in the proximal limbs and shoulder or pelvic girdles, the subtypes of LGMD do not share many features in common. The group is classified, by mode of inheritance, into LGMD1 (autosomal dominant) and LGMD2 (autosomal recessive). The forms are further sub grouped based on the causative gene loci. Spontaneous animal models of LGMD are uncommon and the majority of models are in transgenic mice. Since mutations that result in LGMD sometimes result in other phenotypes, many of these animal models are useful beyond the scope of LGMD. In general the animal models of LGMD do not closely resemble the human phenotype, with a few exceptions. Although many of these models do not exactly replicate the human disease, they are still valuable tools in biomedical research, not only to further studies in understanding the mechanisms of the disease, but also to identify and test ...
TY - JOUR. T1 - AMP-dependent kinase inhibits oxidative stress-induced caveolin-1 phosphorylation and endocytosis by suppressing the dissociation between c-Abl and Prdx1 proteins in endothelial cells. AU - Takeuchi, Kimio. AU - Morizane, Yuki. AU - Kamami-Levy, Cynthia. AU - Suzuki, Jun. AU - Kayama, Maki. AU - Cai, Wenyi. AU - Miller, Joan W.. AU - Vavvas, Demetrios G.. PY - 2013/7/12. Y1 - 2013/7/12. N2 - Caveolin-1 is the primary structural component of endothelial caveolae that is essential for transcellular trafficking of albumin and is also a critical scaffolding protein that regulates the activity of signaling molecules in caveolae. Phosphorylation of caveolin-1 plays a fundamental role in the mechanism of oxidant-induced vascular hyper permeability. However, the regulatory mechanism of caveolin-1 phosphorylation remains unclear. Here we identify a previously unexpected role for AMPKin inhibition of caveolin-1 phosphorylation under oxidative stress. A pharmacological activator of AMPK, ...
Lipids are the energy source used during liver regeneration. The research group, led by Dr. Albert Pol and with members from IDIBAPS, Universitat de Barcelona and Queensland University, unveils the essential role of the protein caveolin-1 in a fundamental process for liver cure after injury or transplant. Results also evidence the existence of cellular mechanisms by which excessive accumulation of lipids in the liver is a risk factor for the apparition of hepatic tumours.
Caveolae are specialised forms of lipid rafts in the plasma membrane of most cells. They form dynamic assemblies of sphingolipids and cholesterol containing scaffolding domains with different affinities for proteins resulting in a variety of functions [1]. The constitutive Cav-1 protein is distributed ubiquitously, while Cav-2 is usually associated with Cav-1 [3, 28]. Although Cav-3 is thought to be "muscle specific" and is expressed in striated muscles [3, 28, 29], we and others have found that Cav-3 is not expressed within human ASM [6, 30]. Recent studies have established that ASM caveolae contain a number of proteins important to [Ca2+]i regulation (e.g. agonist receptors, Ca2+ influx channels, and force regulatory proteins such as RhoA). In canine ASM, it has been established that caveolar-enriched membrane fractions express Cav-1, L-type Ca2+ channels and plasma membrane Ca2+ ATPase, but not SR proteins such as IP3R or RyR channels [31].. In ASM of different species, in addition to Ca2+ ...
Garg V, Sun W, Hu K (2009). "Caveolin-3 negatively regulates recombinant cardiac K(ATP) channels". Biochem. Biophys. Res. ... 2008). "Protein kinase C-epsilon induces caveolin-dependent internalization of vascular adenosine 5'-triphosphate-sensitive K+ ... 385 (3): 472-7. doi:10.1016/j.bbrc.2009.05.100. PMID 19481058. ABCC9 human gene location in the UCSC Genome Browser. ABCC9 ... 40 (3): 184-8. doi:10.1152/physiolgenomics.00173.2009. PMID 19952277. Sato N, Nakayama T, Asai S, Soma M (2006). "A haplotype ...
... has been shown to interact with Caveolin 3 in skeletal muscle., and this interaction is thought to retain dysferlin ... Hernández-Deviez DJ, Howes MT, Laval SH, Bushby K, Hancock JF, Parton RG (2008). "Caveolin regulates endocytosis of the muscle ... Dysferlin also interacts with MG53, and a functional interaction between dysferlin, caveolin-3 and MG53 is thought to be ... caveolin-3, and dysferlin". J. Biol. Chem. 284 (23): 15894-902. doi:10.1074/jbc.M109.009589. PMC 2708885 . PMID 19380584. ...
Scherer PE, Lisanti MP (Aug 1997). "Association of phosphofructokinase-M with caveolin-3 in differentiated skeletal myotubes. ... 63 (3): 1154-65. doi:10.2337/db13-1301. PMC 3931395 . PMID 24306210. Su Y, Zhou A, Al-Lamki RS, Karet FE (May 2003). "The a- ... 61 (3): 415-9. doi:10.1016/0009-8981(75)90434-9. PMID 125160. Zhao ZZ, Malencik DA, Anderson SR (Feb 1991). "Protein-induced ...
Identification of a central WW-like domain within caveolin family members". The Journal of Biological Chemistry. 275 (48): ... Caveolin 3 and SHC1. Actin-binding protein Agrin GRCh38: Ensembl release 89: ENSG00000173402 - Ensembl, May 2017 GRCm38: ... "Caveolin-3 directly interacts with the C-terminal tail of beta -dystroglycan. ... 23 (3): 338-42. doi:10.1038/15519. PMID 10610181. Rentschler S, Linn H, Deininger K, Bedford MT, Espanel X, Sudol M (Apr 1999 ...
IGFBP-3 is one of six IGF binding proteins (IGFBP-1 to IGFBP-6) that have highly conserved structures and bind the insulin-like ... The main IGFBP-3 ligands in the circulation are IGF-1 and IGF-2, and the acid-labile subunit (ALS). The serum proteins ... IGFBP-3 exerts antiproliferative effects in many cell types by blocking the ability of IGF-1 and IGF-2 to activate the IGF1R ( ... Since IGFBP-3 is abundant in the bloodstream of healthy adults (typically 2-4 mg/L), and is largely stabilized by its complex ...
"Interaction of synthetic peptides corresponding to the scaffolding domain of Caveolin-3 with model membranes". Biopolymers. 84 ... doi:10.1016/s0196-9781(01)00628-3. PMID 11835989. CS1 maint: Multiple names: authors list (link) Pallavi, B.; Nagaraj, R. (2003 ...
Zhao G, Simpson RU (2010). "Membrane Localization, Caveolin-3 Association and Rapid Actions of Vitamin D Receptor in Cardiac ... 49 (3): 668-73. PMC 1683124 . PMID 1652893. Szpirer J, Szpirer C, Riviere M, Levan G, Marynen P, Cassiman JJ, Wiese R, DeLuca ... 60 Suppl 3: 106-10. PMID 11979895. Uitterlinden AG, Fang Y, Van Meurs JB, Pols HA, Van Leeuwen JP (2004). "Genetics and biology ... 3 (3): 361-70. doi:10.1016/S1097-2765(00)80463-3. PMID 10198638. Tagami T, Lutz WH, Kumar R, Jameson JL (December 1998). "The ...
LQT9 This newly discovered variant is caused by mutations in the membrane structural protein, caveolin-3. Caveolins form ... 35 (3): e62-e64. doi:10.1111/j.1540-8159.2010.02913.x. ISSN 0147-8389. Wang F, Liu J, Hong L, et al. (2013). "The phenotype ... 89 (3): 137-46. doi:10.1002/(SICI)1096-8628(19990924)89:3. 3.0.CO;2-C. PMID 10704188. Jervell A, Lange-Nielsen F; Lange-Nielsen ... 110 (3): 381-8. doi:10.1172/JCI15183. PMC 151085 . PMID 12163457. Shah, Dipak P.; Baez-Escudero, Jose L.; Weisberg, Ian L.; ...
Czarny M, Fiucci G, Lavie Y, Banno Y, Nozawa Y, Liscovitch M (2000). "Phospholipase D2: functional interaction with caveolin in ... functional interaction with caveolin in low-density membrane microdomains". FEBS Lett. 467 (2-3): 326-32. doi:10.1016/s0014- ... Caveolin 1, Glyceraldehyde 3-phosphate dehydrogenase, PLCG1, PRKCD, Src, and Wiskott-Aldrich syndrome protein. N-(2-(1-(3- ... 467 (2-3): 326-32. doi:10.1016/S0014-5793(00)01174-1. PMID 10675563. Lee C, Kim SR, Chung JK, Frohman MA, Kilimann MW, Rhee SG ...
2005). "Junctophilin type 2 is associated with caveolin-3 and is down-regulated in the hypertrophic and dilated ... 325 (3): 852-6. doi:10.1016/j.bbrc.2004.10.107. PMID 15541368. Kim J, Bhinge AA, Morgan XC, Iyer VR (2005). "Mapping DNA- ... 273 (3): 920-7. doi:10.1006/bbrc.2000.3011. PMID 10891348. Garbino A, van Oort RJ, et al. (2009). "Molecular evolution of the ... 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. Matsushita Y, Furukawa T, Kasanuki H, et al. (2007). "Mutation ...
G-protein-coupled receptor signaling components localize in both sarcolemmal and intracellular caveolin-3-associated ... 5(3):237-48. García-Marcos, M., Ghosh, P., and M.G. Farquhar. 2009. GIV is a non-receptor GEF factor for Galphai with a unique ... 2006 Jul 3; PMID 16818493 Farquhar, M. G. 2006. The glomerular basement membrane: not gone, just forgotten. J. Clin. Invest. ...
Llorente A, de Marco MC, Alonso MA (Oct 2004). "Caveolin-1 and MAL are located on prostasomes secreted by the prostate cancer ... Millán J, Puertollano R, Fan L, Alonso MA (Apr 1997). "Caveolin and MAL, two protein components of internal detergent-insoluble ... 233 (3): 707-12. doi:10.1006/bbrc.1997.6530. PMID 9168919. Martín-Belmonte F, Kremer L, Albar JP, Marazuela M, Alonso MA (Apr ... 82 (3): 550-62. doi:10.1046/j.1471-4159.2002.00987.x. PMID 12153479. Copie-Bergman C, Plonquet A, Alonso MA, Boulland ML, ...
Llorente A, de Marco MC, Alonso MA (2005). "Caveolin-1 and MAL are located on prostasomes secreted by the prostate cancer PC-3 ... 19 (3): 925-33. PMID 15168355. Rohan S, Tu JJ, Kao J, et al. (2007). "Gene expression profiling separates chromophobe renal ... 76 (1-3): 81-8. doi:10.1006/geno.2001.6610. PMID 11549320. "Entrez Gene: MAL2 mal, T-cell differentiation protein 2". Marazuela ... 332 (3): 675-87. doi:10.1016/S0022-2836(03)00944-6. PMID 12963375. Strausberg RL, Feingold EA, Grouse LH, et al. (2003). " ...
The C-terminus of DLC1 is also known to interact with caveolin-1, although the biological significance of this interaction has ... caveolin-1, DLC-1, and NM23-H2 as putative antitumorigenic, progesterone-regulated genes for ovarian cancer cells by gene ... The detailed pathways by which DLC1 results in the cleavage of procaspase-3 and decrease in Bcl-2 levels require further ... DLC1 is responsible for inducing programmed cell death by at least two mechanisms: caspase-3-mediated apoptosis and Bcl-2 ...
Caveolin-3 Association and Rapid Actions of Vitamin D Receptor in Cardiac Myocytes". Steroids 75 (8-9): 555-9. PMC 2885558. ... doi:10.1016/S1097-2765(00)80463-3. *^ a b Tagami T, Lutz WH, Kumar R, Jameson JL (December 1998). "The interaction of the ... Sinteza receptora vitamina vitamina D3 je genetički kontrolrana. Ovaj receptor istovremeno funkcionira i kao sekundarna žučna i ... 3)-induced differentiation by sequestering the vitamin D(3) receptor". Cancer Res. 62 (23): 7050-8. PMID 12460926. ...
... caveolin 1 MeSH D12.776.543.990.100.750 -- caveolin 2 MeSH D12.776.543.990.100.875 -- caveolin 3 MeSH D12.776.543.990.150.100 ... toll-like receptor 3 MeSH D12.776.543.750.705.910.500.400 -- toll-like receptor 4 MeSH D12.776.543.750.705.910.500.500 -- toll- ... erbb-3 MeSH D12.776.543.750.060.468 -- receptor, igf type 1 MeSH D12.776.543.750.060.484 -- receptor, insulin MeSH D12.776. ... type 3 MeSH D12.776.543.750.750.400.370.937 -- receptor, fibroblast growth factor, type 4 MeSH D12.776.543.750.750.400.370.968 ...
There are two isoforms of caveolin-1: caveolin-1α and caveolin-1β, the latter lacking a part of the N-terminus. Caveolins are ... The expression pattern of caveolin-2 is similar to that of caveolin-1; it seems to be co-expressed with caveolin-1. The ... The caveolin gene family has three members in vertebrates: CAV1, CAV2, and CAV3, coding for the proteins caveolin-1, caveolin-2 ... Caveolin-1 has also been shown to play a role in the integrin signaling. The tyrosine phosphorylated form of caveolin-1 ...
... within caveolin-1 molecule. Molecules that interact with caveolin-1 contain caveolin-binding motifs (CBM). Caveolin GRCh38: ... "Human caveolin-1 and caveolin-2 are closely linked genes colocalized with WI-5336 in a region of 7q31 frequently deleted in ... Caveolin 2, PLD2, Bruton's tyrosine kinase and SCP2. All these interactions are through a caveolin-scaffolding domain (CSD) ... caveolin. Caveolin binding negatively regulates the auto-activation of Src tyrosine kinases". J. Biol. Chem. 271 (46): 29182-90 ...
Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting ... "Entrez Gene: CAV3 caveolin 3". Hedley PL, Kanters JK, Dembic M, Jespersen T, Skibsbye L, Aidt FH, Eschen O, Graff C, Behr ER, ... Caveolin-3 is a protein that in humans is encoded by the CAV3 gene. Alternative splicing has been identified for this locus, ... Caveolin 3 has been shown to interact with a range of different proteins, including, but not limited to: DAG1, DYSF, EGFR, and ...
... has been shown to interact with Caveolin 1 and RAS p21 protein activator 1. GRCh38: Ensembl release 89: ... 1998). "Expression of caveolin-1 and -2 in differentiating PC12 cells and dorsal root ganglion neurons: caveolin-2 is up- ... Fra AM, Mastroianni N, Mancini M, Pasqualetto E, Sitia R (May 1999). "Human caveolin-1 and caveolin-2 are closely linked genes ... 2002). "Epithelial expression of caveolin-2, but not caveolin-1, is enhanced in the inflamed mucosa of patients with ulcerative ...
RhoC GTPase is overexpressed, possibly related to overexpression (hypomethylation) of caveolin-1 and -2. Caveolin is ... caveolin-1 and -2 are overexpressed and may contribute to tumour cell motility[13] ... "Overexpression of caveolin-1 and -2 in cell lines and in human samples of inflammatory breast cancer". Breast Cancer Research ... ISBN 978-3-0348-0836-1.. *^ "Facts for Life - Inflammatory Breast Cancer" (PDF). Susan G. Komen for the Cure. Retrieved 2006-12 ...
... co-immunoprecipitates with src kinases and caveolin". Pancreas. 21 (3): 219-25. doi:10.1097/00006676-200010000-00001. PMID ... 1491 (1-3): 376-80. doi:10.1016/S0167-4781(00)00057-9. PMID 10760606. Parker EM, Zaman MM, Freedman SD (2001). "GP2, a GPI- ... J. 277 (3): 879-81. PMC 1151326 . PMID 1651706. Fukuoka S, Freedman SD, Scheele GA (1991). "A single gene encodes membrane- ... 79 (3-4): 231-2. doi:10.1159/000134730. PMID 9605860. "Entrez Gene: GP2 glycoprotein 2 (zymogen granule membrane)". Jacob M, ...
Caveolin 1 is dephosphorylated on tyrosine 14 in response to shear stress and PTPmu is hypothesized to catalyze this reaction. ... Caveolin 1 is a scaffolding protein enriched in endothelial cell junctions that is also linked to shear stress regulated ... Shin J, Jo H, Park H (2006). "Caveolin-1 is transiently dephosphorylated by shear stress-activated protein tyrosine phosphatase ... 339 (3): 737-41. doi:10.1016/j.bbrc.2005.11.077. PMID 16325778. Fuchs M, Wang H, Ciossek T, Chen Z, Ullrich A (1998). " ...
This gene codes for the Caveolin protein, which is a scaffolding membrane protein. This protein plays a role in lipid ... April 2008). "Association of a Homozygous Nonsense Caveolin-1 Mutation with Berardinelli-Seip Congenital Lipodystrophy". ... More recently, type 3 CGL was identified as a separate type of CGL, which was identified as a mutation in the CAV1 gene. Then, ... Types 3 and 4 are two different mutations but they share a common defective pathway. Medical diagnosis of CGL can be made after ...
Feng X, Gaeta ML, Madge LA, Yang JH, Bradley JR, Pober JS (March 2001). "Caveolin-1 associates with TRAF2 to form a complex ... Caveolin 1, EDARADD, HIVEP3, IKK2, Low affinity nerve growth factor receptor, MAP3K14, MAP3K1 MAP3K7IP2, MAP4K2, MAP4K5, RANK, ... "A phosphotyrosine-dependent protein interaction screen reveals a role for phosphorylation of caveolin-1 on tyrosine 14: ... 2 (3): 389-95. doi:10.1016/s1097-2765(00)80283-x. PMID 9774977. Hoeflich KP, Yeh WC, Yao Z, Mak TW, Woodgett JR (October 1999 ...
978-3-540-13679-8. . PMID 6240757.. *^ Vallbo AB, al-Falahe NA (February 1990). "Human muscle spindle response in a motor ... 3) Fusimotor template of intended movement. Static gamma activity is a "temporal template" of the expected shortening and ... 571 (Pt 3): 711-23. doi:10.1113/jphysiol.2005.101634. PMC 1805796. PMID 16423858.. ...
Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting ... "Entrez Gene: CAV3 caveolin 3". Hedley PL, Kanters JK, Dembic M, Jespersen T, Skibsbye L, Aidt FH, Eschen O, Graff C, Behr ER, ... Caveolin-3 is a protein that in humans is encoded by the CAV3 gene. Alternative splicing has been identified for this locus, ... Caveolin 3 has been shown to interact with a range of different proteins, including, but not limited to: DAG1, DYSF, EGFR, and ...
Abcam provides specific protocols for Anti-Caveolin-3 antibody (ab87770) : Western blot protocols, Immunohistochemistry ...
... caveolin-1α and -1β, caveolin-2, and caveolin-3). Caveolin-1 and -2 are coexpressed and abundantly expressed in adipocytes, ... Caveolin-mediated regulation of signaling along the p42/44 MAP kinase cascade in vivo: a role for the caveolin-scaffolding ... Several caveolin-related proteins have been shown to bind to caveolins through caveolin-binding domains (øXøXXXXø or øXXXXøXXø ... and found that they did not have caveolin-binding domains. This suggests that JNK is not regulated by caveolin. ...
Identification, sequence, and expression of caveolin-2 defines a caveolin gene family. Proc. Natl. Acad. Sci. USA 93, 131-135 ( ... Aberrant dysferlin trafficking in cells lacking caveolin or expressing dystrophy mutants of caveolin-3. Hum. Mol. Genet. 15, ... Way, M. & Parton, G. M-caveolin, a muscle-specific caveolin-related protein. FEBS Lett. 376, 108-112 (1995). ... Byrne, D. P., Dart, C. & Rigden, D. J. Evaluating caveolin interactions: do proteins interact with the caveolin scaffolding ...
Tags: Caveolin-3, Duchenne Muscular Dystrophy, Dystrophin, Jerry Lewis, Muscular Dystrophy Association, Niemann Pick Type C, ... You are here: Home / Archives for Caveolin-3. Calling Jerry Lewis - Scientists Report Understanding Niemann Pick Type C Could ...
The Caveolin-3 is purified by proprietary chromatographic techniques. ... Recombinant Human Caveolin-3 full length protein expressed in E.coli, shows a 50 kDa band on SDS-PAGE (Including GST). ... Three proteins, caveolin-1, caveolin-2 and caveolin-3 have been identified as principle components of caveolae.. ... Recombinant Human caveolin 3. Download Datasheet See All CAV3 Products. Bring this labeled protein directly to your bench! ...
Abstract 21013: Caveolin 3-Containing Lipid Rafts Synergized With Insulin to Activate Akt Kinase by Blocking Akt ... Abstract 21013: Caveolin 3-Containing Lipid Rafts Synergized With Insulin to Activate Akt Kinase by Blocking Akt ... Abstract 21013: Caveolin 3-Containing Lipid Rafts Synergized With Insulin to Activate Akt Kinase by Blocking Akt ... Abstract 21013: Caveolin 3-Containing Lipid Rafts Synergized With Insulin to Activate Akt Kinase by Blocking Akt ...
Previous experiments have demonstrated that membrane proteins interact with caveolin scaffolding domain via a specific caveolin ... Role of caveolin and caveolae in insulin signaling and diabetes. Am J Physiol Endocrinol Metab. 2003;285:E1151-E1160. ... Caveolin gene transfer improves glucose metabolism in diabetic mice. Am J Physiol Cell Physiol. 2010;298:C450-C456. ... Essential Role of Caveolin-3 in Adiponectin Signalsome Formation and Adiponectin Cardioprotection. Yajing Wang, Xiaoliang Wang ...
Abstract 15887: MURC/cavin-4 Functions as a Negative Regulator of Caveolin-3 in Tte Development of Heart Failure. Daisuke Naito ... Conclusion: MURC functions as a negative regulator of caveolin-3 at caveolae and modulates localization of caveolin-3 in CMs. ... MURC was also colocalized and associated with caveolin-3 at the plasma membrane of CMs, suggesting an inhibitory interaction of ... MURCΔCC impaired membrane localization of caveolin-3, resulting in increased cAMP levels in CMs compared with MURC. Transgenic ...
Several mutations in the muscle-specific caveolin, caveolin-3, lead to a form of autosomal dominant muscular dystrophy referred ... A caveolin-3 mutant that causes limb girdle muscular dystrophy type 1C disrupts Src localization and activity and induces ... Caveolin 3. Caveolins / genetics*, metabolism. Cells, Cultured. Membrane Microdomains / metabolism. Mice. Microscopy, ... The TFT mutation reduced the binding of Src to caveolin-3, diminished targeting of Src to lipid rafts, and caused abnormal ...
Conclusion: Overall, we show for the first time that a high palmitate diet leads to loss of caveolin-3 in cardiomyocytes and to ... To determine the mechanism of the loss of plasma membrane bound eNOS, we investigated the effect of palmitate on caveolin-3 and ... Ceramide is a sphingolipid and localizes to caveolae, which are lined in the inner membrane leaflet by caveolin proteins. In ... The remaining 30% of caveolin-3 was localized to a peri-nuclear location. In contrast to previous studies, palmitate did not ...
TRPC1 binds to caveolin-3 and is regulated by Src kinase - role in Duchenne muscular dystrophy ... TRPC1 binds to caveolin-3 and is regulated by Src kinase - role in Duchenne muscular dystrophy ... TRPC1 binds to caveolin-3 and is regulated by Src kinase - role in Duchenne muscular dystrophy ... TRPC1 binds to caveolin-3 and is regulated by Src kinase - role in Duchenne muscular dystrophy ...
The Role of CAV3 in Long-QT Syndrome Clinical and Functional Assessment of a Caveolin-3/Kv11.1 Double Heterozygote Versus ... The Role of CAV3 in Long-QT Syndrome Clinical and Functional Assessment of a Caveolin-3/Kv11.1 Double Heterozygote Versus ... The Role of CAV3 in Long-QT Syndrome Clinical and Functional Assessment of a Caveolin-3/Kv11.1 Double Heterozygote Versus ... Caveolin-3 Single Heterozygote. CIRCULATION-CARDIOVASCULAR GENETICS , 6 (5) pp. 452-461. 10.1161/CIRCGENETICS.113.000137. ...
A deficiency in caveolin-3 (Cav-3), the major striated muscle isoform, is responsible for skeletal muscle disorders, such as ... Here we show that a loss of Cav-3 induced by the expression of the LGMD 1C-associated mutant P104L (Cav-3(P104L)) provokes a ... In myotubes expressing Cav-3(P104L), the loss of Cav-3 was accompanied by a 66% reduction in Ca(v)1.1 mean labelling intensity ... Confocal immunomiscrocopy indicated a colocalization of Cav-3 and Ca(v)1.1, the pore-forming subunit of the L-type Ca(2+) ...
... signaling is dependent on caveolin-1; however, it is not known whether caveolin-1 is associated with the beta(3)-AR or solely ... Interaction with Caveolin-1 Modulates G Protein Coupling of Mouse beta(3)-Adrenoceptor. Sato, Masaaki Stockholm University, ... The endogenous beta(3a)-AR displayed G alpha(i/o) coupling in brown adipocytes from caveolin-1 knock-out mice or in wild type ... Our studies indicate that interaction of the beta(3a)-AR with caveolin inhibits coupling to G alpha(i/o) proteins and suggest ...
Caveolin-3 is a muscle specific scaffolding protein with both structural and signaling roles. Lack of caveolin-3 expression has ... In differentiated caveolin-3 null myotubes, agrin treatment yields a 60% reduction in nAChR clusters as compared to agrin ... Hezel, Michael P. (2009) LGMD-1C: Role of Caveolin-3 in Neuromuscular Junction Structure and Function. Doctoral Dissertation, ... Immunoprecipitation of MuSK shows that caveolin-3 and MuSK association peaks at 1 hour of agrin treatment in wildtype cells. ...
Caveolin-3 (CAV3) is a muscle-specific protein localized to the sarcolemma. It was suggested that CAV3 is involved in the ... p.P104L Caveolin-3 causes Golgi/ER stress. Mis-localization of CAV3 aggregates (formed by mutant and wild-type protein) to the ... Caveolin-3 T78M and T78K missense mutations lead to different phenotypes in vivo and in vitro. Lab Investig. 2008;88:275-83. ... Caveolin-3 plays a critical role in autophagy after ischemia-reperfusion. Am J Physiol Cell Physiol. 2016;311:C854-65.View ...
Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting ... The interaction of caveolin 3 protein with the potassium inward rectifier channel Kir2.1: physiology and pathology related to ... This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell ... Identification and functional analysis of a new putative caveolin-3 variant found in a patient with sudden unexplained death.. ...
Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting ... This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell ...
The caveolin gene family has three members in vertebrates: CAV1, CAV2, and CAV3, coding for the proteins caveolin-1, caveolin-2 ... There are two isoforms of caveolin-1: caveolin-1α and caveolin-1β, the latter lacking a part of the N-terminus. ... The expression pattern of caveolin-2 is similar to that of caveolin-1; it seems to be co-expressed with caveolin-1. ... Caveolin-1 has also been shown to play a role in the integrin signaling. The tyrosine phosphorylated form of caveolin-1 ...
Recombinant Human Caveolin-3 protein Full length protein datasheet (ab114264). Abcam offers quality products including ... Recombinant Human Caveolin-3 protein. Caveolin-3 タンパク質・ペプチド 製品一覧. ... お問い合わせは電話 +81-(0)3-6231-0940 または メー
Mammals have three caveolin proteins:caveolin-1 (Cav-1, or VIP21), caveolin-2 and caveolin-3 (or M-caveolin). Various classes ... Evaluating caveolin interactions: do proteins interact with the caveolin scaffolding domain through a widespread aromatic ... Caveolae require the caveolin protein for formation. Caveolins may act as scaffolding proteins within caveolar membranes by ...
MURC/cavin-4 is co-expressed with Caveolin-3 in rhabdomyosarcoma tumors and its silencing prevents myogenic differentiation in ... MURC/cavin-4 is co-expressed with Caveolin-3 in rhabdomyosarcoma tumors and its silencing prevents myogenic differentiation in ... MURC/cavin-4 is co-expressed with Caveolin-3 in rhabdomyosarcoma tumors and its silencing prevents myogenic differentiation in ... MURC/cavin-4 is co-expressed with Caveolin-3 in rhabdomyosarcoma tumors and its silencing prevents myogenic differentiation in ...
Caveolin-2兔单克隆抗体[EPR2220(3)](ab124883)可与人样本反应并经WB, Flow Cyt实验严格验证。中国75% ... All lanes : Anti-Caveolin-2 (phospho Y19) antibody [EPR2220(3)] (ab124883) at 1/1000 dilution. Lane 1 : HUVEC cell lysate ... Anti-Caveolin-2 (phospho Y19)抗体[EPR2220(3)]. 参阅全部 Caveolin-2 一抗. ... Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding
Rabbit Monoclonal Anti-Caveolin-1 Antibody (SP43). Caveolae Marker, Endosome Marker. Validated: IHC, IHC-P. Tested Reactivity: ... The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle ... Caveolin-1 Antibody (SP43) Summary. Immunogen. Synthetic peptide corresponding to C-terminus of human Caveolin 1 protein ... Blogs on Caveolin-1. There are no specific blogs for Caveolin-1, but you can read our latest blog posts. ...
  • We hypothesized that aquaporin 3 is involved in the regulation of keratinocyte function by a mechanism involving the interaction between aquaporin 3 and phospholipase D. Using sucrose gradient centrifugation, immunoprecipitation analysis, and confocal microscopy, we found that aquaporin 3 and phospholipase D 2 colocalized in caveolin-rich membrane microdomains. (elsevier.com)
  • Zheng, X & Bollag, WB 2003, ' Aquaporin 3 Colocates with Phospholipase D 2 in Caveolin-Rich Membrane Microdomains and Is Downregulated Upon Keratinocyte Differentiation ', Journal of Investigative Dermatology , vol. 121, no. 6, pp. 1487-1495. (elsevier.com)
  • The phosphatidylinositol 3' kinase (PI3K)/AKT axis, Hedgehog signaling pathway, nuclear factor-kappaB and Activating Transcription Factor 2 have also been implicated to be involved in EMT. (wikipedia.org)
  • The molecular mechanisms underlying caveolin-associated diseases are still poorly understood. (nature.com)
  • Molecular and cellular experiments revealed that APN receptor 1 (AdipoR1) colocalized with Cav-3, forming AdipoR1/Cav-3 complex via specific Cav-3 scaffolding domain binding motifs. (ahajournals.org)
  • Therefore, the aims of the present study were to (1) determine the role of caveolin-3 (Cav-3) (the predominant form of caveolin expressed in cardiomyocytes) in the cardioprotective actions of APN, and (2) investigate the molecular mechanisms responsible for Cav-3 regulation of APN transmembrane signaling. (ahajournals.org)
  • While the molecular mechanisms involved in nAChR clustering remain to be fully defined, we hypothesize caveolin-3 is important for nAChR clustering and overall neuromuscular junction function.Caveolin-3 and the nAChR co-localize and associate evidenced by immunofluorescence and immunoprecipitation. (pitt.edu)
  • In hearts with MI, BDNF/TrkB relieved myocardial ischemic injury and suppressed myocardial cell apoptosis via the regulation of transient receptor potential canonical (TRPC) 3/6 channels, thus suggesting a new underlying therapy for MI [ 6 ]. (aging-us.com)
  • This checkpoint function of caveolin-1 explains its effect on diverse signaling pathways regulating cell proliferation, differentiation, apoptosis, adhesion, and invasion. (aacrjournals.org)
  • Its re-expression induced apoptosis and cell cycle arrest and resulted in a mesenchymal-to-epithelial transition, such as up-regulation of E-cadherin and reduction of cell locomotion and invasion, and in the down-regulation of several oncogenes known to be involved in disease progression (i.e., interleukin 6, caveolin-1, EZH2). (wikipedia.org)
  • However, in contrast to the latter group of animals, the dysferlin-deficient mice have an intact dystrophin glycoprotein complex and normal levels of caveolin-3. (nih.gov)
  • Mutant beta(3a)-ARs (F389A/Y392A/F398A or P384S/F389A) promoted PTX-sensitive cAMP responses, and in situ proximity assays demonstrated an association between caveolin-1 and the wild type beta(3a)-AR but not the mutant receptors. (diva-portal.org)
  • The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). (wikipedia.org)
  • Unlike most enveloped viruses which use clathrin coated pits for cellular entry and bind to their receptors in a pH dependent fashion, Lassa and lymphocytic choriomeningitis virus instead use an endocytotic pathway independent of clathrin, caveolin, dynamin and actin. (wikipedia.org)
  • These results indicate a role for caveolin-3 in efficient nAChR clustering.Electromyography studies in anesthetized mice indicated lengthened latencies of the muscle action potential in the caveolin-3 null mice as compared to wildtype mice. (pitt.edu)
  • There were also decreased overall electromyography (EMG) amplitude and EMG area under the curve in caveolin-3 null mice. (pitt.edu)
  • Aquaporin 3-deficient mice exhibit skin defects, including decreased glycerol content and impairment of water holding capacity, barrier recovery, and wound healing. (elsevier.com)
  • Vital staining with Evans blue dye revealed loss of sarcolemmal integrity in both lines of mice, similar to that seen in mdx and caveolin-3 deficient mice. (nih.gov)
  • As caveolin-2 expression is also severely reduced in Cav-1-null mice, we conclude that caveolin-2 deficiency is the clear culprit in this lung disorder. (asm.org)
  • It has been shown that Caveolin-1-deficient mice show insulin resistance. (wikipedia.org)
  • MicroRNA-103/107 inhibition in Caveolin-1-deficient mice has no effect on insulin sensitivity and signalling. (wikipedia.org)
  • However, the cellular mechanism by which loss of caveolin 3 leads to muscle atrophy is unknown. (fujita-hu.ac.jp)
  • However, recent studies have suggested that insulin signaling may be an exception, which requires the presence of caveolin for transmembrane signaling. (ahajournals.org)
  • The presence of caveolin leads to a local change in morphology of the membrane. (wikipedia.org)
  • Caveolin-1 has also been shown to play a role in the integrin signaling. (wikipedia.org)
  • There are dedicated phagocytotic integrins, such as integrin αMβ2 (complement receptor 3), that internalize particulate material ( Dupuy and Caron, 2008 ). (biologists.org)
  • Intriguingly, the normal increases in the transcript of caveolin 3 as well as an integrin subunit, the beta1D isoform, were suppressed by FAK inhibition. (unitn.it)
  • The stimulation of I Ca,L in response to β 2 -AR activation was eliminated by disruption of caveolae with 10 mM methyl β-cyclodextrin or by small interfering RNA directed against caveolin-3, whereas β 1 -AR stimulation (norepinephrine plus prazosin) of I Ca,L was not altered. (pnas.org)
  • Here, we show that subcytotoxic oxidative stress generated by hydrogen peroxide application promotes premature senescence and stimulates the activity of a (−1,296) caveolin-1 promoter reporter gene construct in fibroblasts. (aacrjournals.org)
  • Several studies implicate that caveolin-1 fulfills a tumor-suppressor role in vitro and in vivo ( 8 - 10 ). (aacrjournals.org)