Caveolin 2 is a binding partner of CAVEOLIN 1. It undergoes tyrosine phosphorylation by C-SRC PROTEIN PP60 and plays a regulatory role in CAVEOLAE formation.
Endocytic/exocytic CELL MEMBRANE STRUCTURES rich in glycosphingolipids, cholesterol, and lipid-anchored membrane proteins that function in ENDOCYTOSIS (potocytosis), transcytosis, and SIGNAL TRANSDUCTION. Caveolae assume various shapes from open pits to closed vesicles. Caveolar coats are composed of CAVEOLINS.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Detergent-insoluble CELL MEMBRANE components. They are enriched in SPHINGOLIPIDS and CHOLESTEROL and clustered with glycosyl-phosphatidylinositol (GPI)-anchored proteins.
A complex of polyene antibiotics obtained from Streptomyces filipinensis. Filipin III alters membrane function by interfering with membrane sterols, inhibits mitochondrial respiration, and is proposed as an antifungal agent. Filipins I, II, and IV are less important.
Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.
A partitioning within cells due to the selectively permeable membranes which enclose each of the separate parts, e.g., mitochondria, lysosomes, etc.
The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.
Cyclic GLUCANS consisting of seven (7) glucopyranose units linked by 1,4-glycosidic bonds.
The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. Each molecule of clathrin consists of three light chains (CLATHRIN LIGHT CHAINS) and three heavy chains (CLATHRIN HEAVY CHAINS) that form a structure called a triskelion. Clathrin also interacts with cytoskeletal proteins.
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Nonionic surfactant mixtures varying in the number of repeating ethoxy (oxy-1,2-ethanediyl) groups. They are used as detergents, emulsifiers, wetting agents, defoaming agents, etc. Octoxynol-9, the compound with 9 repeating ethoxy groups, is a spermatocide.
A homologous group of cyclic GLUCANS consisting of alpha-1,4 bound glucose units obtained by the action of cyclodextrin glucanotransferase on starch or similar substrates. The enzyme is produced by certain species of Bacillus. Cyclodextrins form inclusion complexes with a wide variety of substances.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Established cell cultures that have the potential to propagate indefinitely.
A CALCIUM-dependent, constitutively-expressed form of nitric oxide synthase found primarily in ENDOTHELIAL CELLS.
Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays.
Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.
Neoplasms composed of fatty tissue or connective tissue made up of fat cells in a meshwork of areolar tissue. The concept does not refer to neoplasms located in adipose tissue.
A subtype of dynamin found ubiquitously expressed in a variety of tissues.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Cells in the body that store FATS, usually in the form of TRIGLYCERIDES. WHITE ADIPOCYTES are the predominant type and found mostly in the abdominal cavity and subcutaneous tissue. BROWN ADIPOCYTES are thermogenic cells that can be found in newborns of some species and hibernating mammals.
Compounds containing carbohydrate or glycosyl groups linked to phosphatidylinositols. They anchor GPI-LINKED PROTEINS or polysaccharides to cell membranes.
Membrane-limited structures derived from the plasma membrane or various intracellular membranes which function in storage, transport or metabolism.
Short tracts of DNA sequence that are used as landmarks in GENOME mapping. In most instances, 200 to 500 base pairs of sequence define a Sequence Tagged Site (STS) that is operationally unique in the human genome (i.e., can be specifically detected by the polymerase chain reaction in the presence of all other genomic sequences). The overwhelming advantage of STSs over mapping landmarks defined in other ways is that the means of testing for the presence of a particular STS can be completely described as information in a database.
A family of high molecular weight GTP phosphohydrolases that play a direct role in vesicle transport. They associate with microtubule bundles (MICROTUBULES) and are believed to produce mechanical force via a process linked to GTP hydrolysis. This enzyme was formerly listed as EC 3.6.1.50.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
An NADPH-dependent enzyme that catalyzes the conversion of L-ARGININE and OXYGEN to produce CITRULLINE and NITRIC OXIDE.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The extension of endometrial tissue (ENDOMETRIUM) into the MYOMETRIUM. It usually occurs in women in their reproductive years and may result in a diffusely enlarged uterus with ectopic and benign endometrial glands and stroma.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Cytoplasmic vesicles formed when COATED VESICLES shed their CLATHRIN coat. Endosomes internalize macromolecules bound by receptors on the cell surface.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Centrifugation using a rotating chamber of large capacity in which to separate cell organelles by density-gradient centrifugation. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The protein constituents of muscle, the major ones being ACTINS and MYOSINS. More than a dozen accessory proteins exist including TROPONIN; TROPOMYOSIN; and DYSTROPHIN.
Amino acids containing an aromatic side chain.
A glucose transport protein found in mature MUSCLE CELLS and ADIPOCYTES. It promotes transport of glucose from the BLOOD into target TISSUES. The inactive form of the protein is localized in CYTOPLASMIC VESICLES. In response to INSULIN, it is translocated to the PLASMA MEMBRANE where it facilitates glucose uptake.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Src-family kinases that associate with T-CELL ANTIGEN RECEPTOR and phosphorylate a wide variety of intracellular signaling molecules.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.
Unsaturated derivatives of the steroid androstane containing at least one double bond at any site in any of the rings.
Transport proteins that carry specific substances in the blood or across cell membranes.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
A PROTEIN-TYROSINE KINASE family that was originally identified by homology to the Rous sarcoma virus ONCOGENE PROTEIN PP60(V-SRC). They interact with a variety of cell-surface receptors and participate in intracellular signal transduction pathways. Oncogenic forms of src-family kinases can occur through altered regulation or expression of the endogenous protein and by virally encoded src (v-src) genes.
A genus of owlet moths of the family Noctuidae. These insects are used in molecular biology studies during all stages of their life cycle.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
An enzyme that catalyzes the oxidation of cholesterol in the presence of molecular oxygen to 4-cholesten-3-one and hydrogen peroxide. The enzyme is not specific for cholesterol, but will also oxidize other 3-hydroxysteroids. EC 1.1.3.6.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A subtype of thioredoxin reductase found primarily in the CYTOSOL.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
A heterogeneous group of inherited MYOPATHIES, characterized by wasting and weakness of the SKELETAL MUSCLE. They are categorized by the sites of MUSCLE WEAKNESS; AGE OF ONSET; and INHERITANCE PATTERNS.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Proteins prepared by recombinant DNA technology.
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.
A 700-kDa cytosolic protein complex consisting of seven equimolar subunits (alpha, beta, beta', gamma, delta, epsilon and zeta). COATOMER PROTEIN and ADP-RIBOSYLATION FACTOR 1 are principle components of COAT PROTEIN COMPLEX I and are involved in vesicle transport between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.
GPI-linked membrane proteins broadly distributed among hematopoietic and non-hematopoietic cells. CD55 prevents the assembly of C3 CONVERTASE or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex.
A low affinity receptor that binds NERVE GROWTH FACTOR; BRAIN-DERIVED NEUROTROPHIC FACTOR; NEUROTROPHIN 3; and neurotrophin 4.
A large group of membrane transport proteins that shuttle MONOSACCHARIDES across CELL MEMBRANES.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles is covered with a lattice-like network of the protein CLATHRIN. Shortly after formation, however, the clathrin coat is removed and the vesicles are referred to as ENDOSOMES.
Enzymes that hydrolyze GTP to GDP. EC 3.6.1.-.
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
A specific monosialoganglioside that accumulates abnormally within the nervous system due to a deficiency of GM1-b-galactosidase, resulting in GM1 gangliosidosis.
Lipids containing at least one monosaccharide residue and either a sphingoid or a ceramide (CERAMIDES). They are subdivided into NEUTRAL GLYCOSPHINGOLIPIDS comprising monoglycosyl- and oligoglycosylsphingoids and monoglycosyl- and oligoglycosylceramides; and ACIDIC GLYCOSPHINGOLIPIDS which comprises sialosylglycosylsphingolipids (GANGLIOSIDES); SULFOGLYCOSPHINGOLIPIDS (formerly known as sulfatides), glycuronoglycosphingolipids, and phospho- and phosphonoglycosphingolipids. (From IUPAC's webpage)
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
GTP-BINDING PROTEINS that contain three non-identical subunits. They are found associated with members of the seven transmembrane domain superfamily of G-PROTEIN-COUPLED RECEPTORS. Upon activation the GTP-BINDING PROTEIN ALPHA SUBUNIT of the complex dissociates leaving a dimer of a GTP-BINDING PROTEIN BETA SUBUNIT bound to a GTP-BINDING PROTEIN GAMMA SUBUNIT.
A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.
An essential amino acid that is required for the production of HISTAMINE.
A family of heterotrimeric GTP-binding protein alpha subunits that were originally identified by their ability to inhibit ADENYLYL CYCLASES. Members of this family can couple to beta and gamma G-protein subunits that activate POTASSIUM CHANNELS. The Gi-Go part of the name is also spelled Gi/Go.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
The entering of cells by viruses following VIRUS ATTACHMENT. This is achieved by ENDOCYTOSIS, by direct MEMBRANE FUSION of the viral membrane with the CELL MEMBRANE, or by translocation of the whole virus across the cell membrane.

The dually acylated NH2-terminal domain of gi1alpha is sufficient to target a green fluorescent protein reporter to caveolin-enriched plasma membrane domains. Palmitoylation of caveolin-1 is required for the recognition of dually acylated g-protein alpha subunits in vivo. (1/1802)

Here we investigate the molecular mechanisms that govern the targeting of G-protein alpha subunits to the plasma membrane. For this purpose, we used Gi1alpha as a model dually acylated G-protein. We fused full-length Gi1alpha or its extreme NH2-terminal domain (residues 1-32 or 1-122) to green fluorescent protein (GFP) and analyzed the subcellular localization of these fusion proteins. We show that the first 32 amino acids of Gi1alpha are sufficient to target GFP to caveolin-enriched domains of the plasma membrane in vivo, as demonstrated by co-fractionation and co-immunoprecipitation with caveolin-1. Interestingly, when dual acylation of this 32-amino acid domain was blocked by specific point mutations (G2A or C3S), the resulting GFP fusion proteins were localized to the cytoplasm and excluded from caveolin-rich regions. The myristoylated but nonpalmitoylated (C3S) chimera only partially partitioned into caveolin-containing fractions. However, both nonacylated GFP fusions (G2A and C3S) no longer co-immunoprecipitated with caveolin-1. Taken together, these results indicate that lipid modification of the NH2-terminal of Gi1alpha is essential for targeting to its correct destination and interaction with caveolin-1. Also, a caveolin-1 mutant lacking all three palmitoylation sites (C133S, C143S, and C156S) was unable to co-immunoprecipitate these dually acylated GFP-G-protein fusions. Thus, dual acylation of the NH2-terminal domain of Gi1alpha and palmitoylation of caveolin-1 are both required to stabilize and perhaps regulate this reciprocal interaction at the plasma membrane in vivo. Our results provide the first demonstration of a functional role for caveolin-1 palmitoylation in its interaction with signaling molecules.  (+info)

The Npc1 mutation causes an altered expression of caveolin-1, annexin II and protein kinases and phosphorylation of caveolin-1 and annexin II in murine livers. (2/1802)

We have previously demonstrated (1) an increased expression of caveolin-1 in murine heterozygous and homozygous Niemann-Pick type C (NPC) livers, and (2) an increased concentration of unesterified cholesterol in a detergent insoluble caveolae-enriched fraction from homozygous livers. To define further the relationship between caveolin-1 function and the cholesterol trafficking defect in NPC, we examined the expression and distribution of additional caveolar and signal transduction proteins. The expression of annexin II was significantly increased in homozygous liver homogenates and the Triton X-100 insoluble floating fraction (TIFF). Phosphoamino acid analysis of caveolin-1 and annexin II from the homozygous TIFF demonstrated an increase in serine and tyrosine phosphorylation, respectively. To determine the basis for increased phosphorylation of these proteins, the expression and distribution of several protein kinases was examined. The expression of PKCalpha, PKCzeta and pp60-src (protein kinases) were significantly increased in both heterozygous and homozygous liver homogenates, while PKCdelta was increased only in homozygous livers. Of the protein kinases analyzed, only CK IIalpha was significantly enriched in the heterozygous TIFF. Finally, the concentration of diacylglycerol in the homozygous TIFF was significantly increased and this elevation may modulate PKC distribution and function. These results provide additional evidence for involvement of a caveolin-1 containing cellular fraction in the pathophysiology of NPC and also suggest that the Npc1 gene product may directly or indirectly, regulate the expression and distribution of signaling molecules.  (+info)

Hypercholesterolemia decreases nitric oxide production by promoting the interaction of caveolin and endothelial nitric oxide synthase. (3/1802)

Hypercholesterolemia is a central pathogenic factor of endothelial dysfunction caused in part by an impairment of endothelial nitric oxide (NO) production through mechanisms that remain poorly characterized. The activity of the endothelial isoform of NO synthase (eNOS) was recently shown to be modulated by its reciprocal interactions with the stimulatory Ca2+-calmodulin complex and the inhibitory protein caveolin. We examined whether hypercholesterolemia may reduce NO production through alteration of this regulatory equilibrium. Bovine aortic endothelial cells were cultured in the presence of serum obtained from normocholesterolemic (NC) or hypercholesterolemic (HC) human volunteers. Exposure of endothelial cells to the HC serum upregulated caveolin abundance without any measurable effect on eNOS protein levels. This effect of HC serum was associated with an impairment of basal NO release paralleled by an increase in inhibitory caveolin-eNOS complex formation. Similar treatment with HC serum significantly attenuated the NO production stimulated by the calcium ionophore A23187. Accordingly, higher calmodulin levels were required to disrupt the enhanced caveolin-eNOS heterocomplex from HC serum-treated cells. Finally, cell exposure to the low-density lipoprotein (LDL) fraction alone dose-dependently reproduced the inhibition of basal and stimulated NO release, as well as the upregulation of caveolin expression and its heterocomplex formation with eNOS, which were unaffected by cotreatment with antioxidants. Together, our data establish a new mechanism for the cholesterol-induced impairment of NO production through the modulation of caveolin abundance in endothelial cells, a mechanism that may participate in the pathogenesis of endothelial dysfunction and the proatherogenic effects of hypercholesterolemia.  (+info)

Regulation of G protein-coupled receptor kinases by caveolin. (4/1802)

G protein-coupled receptor kinases (GRKs) have been principally characterized by their ability to phosphorylate and desensitize G protein-coupled receptors. However, recent studies suggest that GRKs may have more diverse protein/protein interactions in cells. Based on the identification of a consensus caveolin binding motif within the pleckstrin homology domain of GRK2, we tested the direct binding of purified full-length GRK2 to various glutathione S-transferase-caveolin-1 fusion proteins, and we discovered a specific interaction of GRK2 with the caveolin scaffolding domain. Interestingly, analysis of GRK1 and GRK5, which lack a pleckstrin homology domain, revealed in vitro binding properties similar to those of GRK2. Maltose-binding protein caveolin and glutathione S-transferase-GRK fusion proteins were used to map overlapping regions in the N termini of both GRK2 and GRK5 that appear to mediate conserved GRK/caveolin interactions. In vivo association of GRK2 and caveolin was suggested by co-fractionation of GRK2 with caveolin in A431 and NIH-3T3 cells and was further supported by co-immunoprecipitation of GRK2 and caveolin in COS-1 cells. Functional significance for the GRK/caveolin interaction was demonstrated by the potent inhibition of GRK-mediated phosphorylation of both receptor and peptide substrates by caveolin-1 and -3 scaffolding domain peptides. These data reveal a novel mode for the regulation of GRKs that is likely to play an important role in their cellular function.  (+info)

Analysis of the CAVEOLIN-1 gene at human chromosome 7q31.1 in primary tumours and tumour-derived cell lines. (5/1802)

We identified CAVEOLIN-1 as a candidate for a tumour suppressor gene mapping to human chromosome 7q31.1. A number of studies suggest that caveolin could function as a tumour suppressor. Expression of caveolin, and in turn the number of caveolae within a cell, are inversely correlated with the transforming ability of numerous oncoproteins, including H-ras, v-abl, and bcr-abl, and caveolin is a major transformation-dependent substrate of v-src. Heterologous expression of caveolin has been shown to abrogate anchorage-independent growth and induce apoptosis in transformed fibroblasts and also to suppress anchorage-independent growth in human mammary carcinoma cells. We have analysed the status and expression of the human CAVEOLIN-1 gene in primary tumours and tumour-derived cell lines. We found no evidence for mutation of CAVEOLIN-1 in human cancers. Additionally, we found that while the first two exons of CAVEOLIN-1 are associated with a CpG island, this is not methylated in either primary tumours or in tumour-derived cell lines in which Caveolin-1 expression is low or undetectable. The level of expression of Caveolin-1 does not correlate with loss of heterozygosity at the CAVEOLIN-1 locus in these same cell lines. Contrary to other published studies, we have shown that CAVEOLIN-1 is not expressed in normal breast ductal epithelial cells in vivo. CAVEOLIN-1 is however highly expressed in breast myoepithelial cells and its expression is retained in tumours derived from breast myoepithelium. Together our data refute a role for CAVEOLIN-1 as a breast tumour suppressor gene in vivo.  (+info)

A role for caveolin and the urokinase receptor in integrin-mediated adhesion and signaling. (6/1802)

The assembly of signaling molecules surrounding the integrin family of adhesion receptors remains poorly understood. Recently, the membrane protein caveolin was found in complexes with beta1 integrins. Caveolin binds cholesterol and several signaling molecules potentially linked to integrin function, e.g., Src family kinases, although caveolin has not been directly implicated in integrin-dependent adhesion. Here we report that depletion of caveolin by antisense methodology in kidney 293 cells disrupts the association of Src kinases with beta1 integrins resulting in loss of focal adhesion sites, ligand-induced focal adhesion kinase (FAK) phosphorylation, and adhesion. The nonintegrin urokinase receptor (uPAR) associates with and stabilizes beta1 integrin/caveolin complexes. Depletion of caveolin in uPAR-expressing 293 cells also disrupts uPAR/integrin complexes and uPAR-dependent adhesion. Further, beta1 integrin/caveolin complexes could be disassociated by uPAR-binding peptides in both uPAR-transfected 293 cells and human vascular smooth muscle cells. Disruption of complexes by peptides in intact smooth muscle cells blocks the association of Src family kinases with beta1 integrins and markedly impairs their migration on fibronectin. We conclude that ligand-induced signaling necessary for normal beta1 integrin function requires caveolin and is regulated by uPAR. Caveolin and uPAR may operate within adhesion sites to organize kinase-rich lipid domains in proximity to integrins, promoting efficient signal transduction.  (+info)

Visualization of caveolin-1, a caveolar marker protein, in living cells using green fluorescent protein (GFP) chimeras. The subcellular distribution of caveolin-1 is modulated by cell-cell contact. (7/1802)

Caveolin-1, a suspected tumor suppressor, is a principal protein component of caveolae in vivo. Recently, we have shown that NIH 3T3 cells harboring anti-sense caveolin-1 exhibit a loss of contact inhibition and anchorage-independent growth. These observations may be related to the ability of caveolin-1 expression to positively regulate contact inhibition. In order to understand the postulated role of caveolin-1 in contact inhibition, it will be necessary to follow the distribution of caveolins in living cells in response to a variety of stimuli, such as cell density. Here, we visualize the distribution of caveolin-1 in living normal NIH 3T3 cells by creating GFP-fusion proteins. In many respects, the behavior of these GFP-caveolin-1 fusion proteins is indistinguishable from endogenous caveolin-1. These GFP-caveolin-1 fusion proteins co-fractionated with endogenous caveolin-1 using an established protocol that separates caveolae-derived membranes from the bulk of cellular membranes and cytosolic proteins, and co-localized with endogenous caveolin-2 in vivo as seen by immunofluorescence microscopy. We show here that as NIH 3T3 cells become confluent, the distribution of GFP-caveolin-1 and endogenous caveolin-1 shifts to areas of cell-cell contact, coincident with contact inhibition. However, unlike endogenous caveolin-1, the levels of GFP-caveolin-1 expression are unaffected by changes in cell density, serum starvation, or growth factor stimulation. These results are consistent with the idea that the levels of endogenous caveolin-1 are modulated by either transcriptional or translational control, and that this modulation is separable from density-dependent regulation of the distribution of caveolin-1. These studies provide a new living-model system for elucidating the dynamic mechanisms underlying the density-dependent regulation of the distribution of caveolin-1 and how this relates to contact inhibition.  (+info)

Tyrosine-phosphorylated caveolin-1: immunolocalization and molecular characterization. (8/1802)

Caveolin-1 was discovered as a major substrate for v-Src, but the effect of its tyrosine phosphorylation has not been known. We generated a specific antibody (PY14) to caveolin-1 phosphorylated at tyrosine 14 and studied the significance of the modification. By Western blotting of lysates of v-Src-expressing cells, PY14 recognized not only a 22-kDa band (the position of nonphosphorylated caveolin-1) but bands at 23-24 and 25 kDa. Bands of slower mobility were diminished by dephosphorylation and were also observed for mutant caveolin-1 lacking tyrosine 14. By immunofluorescence microscopy, PY14 did not label normal cells but detected large dots in v-Src-expressing cells. Immunoelectron microscopy revealed that the dots corresponded to aggregated caveolae and/or vesicles of various sizes; besides, the label was observed in intramembrane particle-free areas in the plasma membrane, which appeared to have been formed by fusion of flattened caveolae. A positive reaction with PY14 was found in normal cells after vanadate or pervanadate treatment; it occurred mainly at 22 kDa by Western blotting and was not seen as large dots by immunofluorescence microscopy. Detergent solubility, oligomerization, and association with caveolin-2 were observed similarly for caveolin-1 in normal and v-Src-expressing cells. The results indicate that phosphorylation of caveolin-1 in v-Src-expressing cells occurs at multiple residues and induces flattening, aggregation, and fusion of caveolae and/or caveolae-derived vesicles.  (+info)

Here are some possible causes of myoglobinuria:

1. Muscle injury or trauma: This can cause myoglobin to leak into the bloodstream and then into the urine.
2. Muscle disease: Certain muscle diseases, such as muscular dystrophy, can cause myoglobinuria.
3. Kidney damage: Myoglobin can accumulate in the kidneys and cause damage if the kidneys are not functioning properly.
4. Sepsis: Sepsis is a systemic infection that can cause muscle breakdown and myoglobinuria.
5. Burns: Severe burns can cause muscle damage and lead to myoglobinuria.
6. Heart attack: A heart attack can cause muscle damage and myoglobinuria.
7. Rhabdomyolysis: This is a condition where the muscles break down and release myoglobin into the bloodstream. It can be caused by various factors such as medication, infection, or injury.

Symptoms of myoglobinuria may include dark urine, proteinuria (excess protein in the urine), and kidney damage. Treatment depends on the underlying cause and may involve supportive care, medication, or dialysis to remove waste products from the blood.

There are several types of adipocytic neoplasms, including:

1. Lipomas: These are benign, slow-growing tumors that are composed of mature fat cells (adipocytes). They are usually soft to the touch and can be moved easily under the skin.
2. Liposarcomas: These are malignant tumors that also originate in adipose tissue. They can be slow-growing or aggressive and can infiltrate surrounding tissues.
3. Pigmented villonodular synovitis (PVN): This is a type of benign tumor that occurs in the synovial membrane, which lines the joints and tendons. It is composed of adipocytes and other cell types and can cause pain and stiffness in the affected joint.
4. Giant cell lipomatosis: This is a rare condition characterized by multiple small lipomas that are clustered together.
5. Spindle cell lipoma: This is a rare type of lipoma that contains spindle-shaped cells, which are elongated and irregular in shape.

These adipocytic neoplasms can be diagnosed through various imaging techniques such as ultrasonography, computed tomography (CT), magnetic resonance imaging (MRI), and fine needle aspiration biopsy. Treatment options vary depending on the type and location of the tumor, but may include surgical excision, radiation therapy, or chemotherapy.

Adenomyosis can cause symptoms such as heavy menstrual bleeding, painful periods, and pelvic pain. Treatment options for adenomyosis include hysterectomy (removal of the uterus), myomectomy (removal of fibroids), and hormonal therapy.

It is important to note that adenomyosis is different from endometriosis, which is a condition where tissue similar to the lining of the uterus grows outside of the uterus, such as on the ovaries or fallopian tubes.

Adenomyosis is a relatively rare condition and its exact cause is not fully understood. However, it is believed that hormonal factors and/or abnormalities in the development of the uterus during fetal development may play a role.

There are several types of muscular dystrophies, including:

1. Duchenne muscular dystrophy (DMD): This is the most common form of muscular dystrophy, affecting males primarily. It is caused by a mutation in the dystrophin gene and is characterized by progressive muscle weakness, wheelchair dependence, and shortened lifespan.
2. Becker muscular dystrophy (BMD): This is a less severe form of muscular dystrophy than DMD, affecting both males and females. It is caused by a mutation in the dystrophin gene and is characterized by progressive muscle weakness, but with a milder course than DMD.
3. Limb-girdle muscular dystrophy (LGMD): This is a group of disorders that affect the muscles around the shoulders and hips, leading to progressive weakness and degeneration. There are several subtypes of LGMD, each with different symptoms and courses.
4. Facioscapulohumeral muscular dystrophy (FSHD): This is a rare form of muscular dystrophy that affects the muscles of the face, shoulder, and upper arm. It is caused by a mutation in the D4Z4 repeat on chromosome 4.
5. Myotonic dystrophy: This is the most common adult-onset form of muscular dystrophy, affecting both males and females. It is characterized by progressive muscle stiffness, weakness, and wasting, as well as other symptoms such as cataracts, myotonia, and cognitive impairment.

There is currently no cure for muscular dystrophies, but various treatments are available to manage the symptoms and slow the progression of the disease. These include physical therapy, orthotics and assistive devices, medications to manage pain and other symptoms, and in some cases, surgery. Researchers are actively working to develop new treatments and a cure for muscular dystrophies, including gene therapy, stem cell therapy, and small molecule therapies.

It's important to note that muscular dystrophy can be inherited in an autosomal dominant, autosomal recessive, or X-linked manner, depending on the specific type of dystrophy. This means that the risk of inheriting the condition depends on the mode of inheritance and the presence of mutations in specific genes.

In summary, muscular dystrophy is a group of genetic disorders characterized by progressive muscle weakness and degeneration. There are several types of muscular dystrophy, each with different symptoms and courses. While there is currently no cure for muscular dystrophy, various treatments are available to manage the symptoms and slow the progression of the disease. Researchers are actively working to develop new treatments and a cure for muscular dystrophy.

All these interactions are through a caveolin-scaffolding domain (CSD) within caveolin-1 molecule. Molecules that interact with ... caveolin. Caveolin binding negatively regulates the auto-activation of Src tyrosine kinases". The Journal of Biological ... Caveolin-1 is a protein that in humans is encoded by the CAV1 gene. The scaffolding protein encoded by this gene is the main ... Caveolin 1 has been shown to interact with heterotrimeric G proteins, Src tyrosine kinases (Src, Lyn) and H-Ras,cholesterol,TGF ...
... caveolin-2, and caveolin-3, respectively. All three members are membrane proteins with similar structure. Caveolin forms ... Furthermore, caveolin-3 has been associated with long QT syndrome. Caveolin-3 has been implicated in the development of certain ... The Caveolin Protein Caveolins at the US National Library of Medicine Medical Subject Headings (MeSH) Caveolin-3 page (Articles ... The expression pattern of caveolin-2 is similar to that of caveolin-1; it seems to be co-expressed with caveolin-1. The ...
... has been shown to interact with Caveolin 1 and RAS p21 protein activator 1. GRCh38: Ensembl release 89: ... 2002). "Epithelial expression of caveolin-2, but not caveolin-1, is enhanced in the inflamed mucosa of patients with ulcerative ... and expression of caveolin-2 defines a caveolin gene family". Proc Natl Acad Sci U S A. 93 (1): 131-5. Bibcode:1996PNAS...93.. ... "Src-induced phosphorylation of caveolin-2 on tyrosine 19. Phospho-caveolin-2 (Tyr(P)19) is localized near focal adhesions, ...
It interacts with caveolin-1. GRCh38: Ensembl release 89: ENSG00000144063 - Ensembl, May 2017 GRCm38: Ensembl release 89: ... 2001). "BENE, a novel raft-associated protein of the MAL proteolipid family, interacts with caveolin-1 in human endothelial- ... 36 (1): 40-5. doi:10.1038/ng1285. PMID 14702039. Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and ... "A novel gene encoding an SH3 domain protein is mutated in nephronophthisis type 1". Nat Genet. 17 (2): 149-53. doi:10.1038/ ...
Lu ML, Schneider MC, Zheng Y, Zhang X, Richie JP (April 2001). "Caveolin-1 interacts with androgen receptor. A positive ... Caveolin 1, CDK9, COX5B, CREB-binding protein, Cyclin D1, Cyclin-dependent kinase 7, DACH1, Death associated protein 6, L-DOPA ... 275 (1): 571-9. doi:10.1074/jbc.275.1.571. PMID 10617653. Liu Y, Kim BO, Kao C, Jung C, Dalton JT, He JJ (May 2004). "Tip110, ... 16 (1): 85-99. doi:10.1210/mend.16.1.0753. PMID 11773441. Pero R, Lembo F, Palmieri EA, Vitiello C, Fedele M, Fusco A, Bruni CB ...
"Tyrosine-phosphorylated Caveolin-1 (Tyr-14) Increases Sensitivity to Paclitaxel by Inhibiting BCL2 and BCLxL Proteins via c-Jun ... "Caveolin-1 Tyrosine Phosphorylation Enhances Paclitaxel-mediated Cytotoxicity". Journal of Biological Chemistry. 282 (8): 5934- ... 8 (1): 37-49. doi:10.1038/nrc2294. PMC 2238676. PMID 18097463. Fan, Ming; Xia, Pingping; Clarke, Robert; Wang, Yue; Li, Lihua ( ... 4 (1): 40. doi:10.1038/s41420-018-0105-y. PMC 6186758. PMID 30345078. Cook, Katherine L.; Shajahan, Ayesha N.; Wärri, Anni; Jin ...
Data from Caveolin-1 knockout mice demonstrated that TLR5 expression significantly decreases in the absence of Caveolin-1 ... It is hypothesized that the Caveolin-1 directly interacts with TLR5 to stabilize it and hence increases the level of TLR5. TLR5 ... Lim JS, Nguyen KC, Han JM, Jang IS, Fabian C, Cho KA (December 2015). "Direct Regulation of TLR5 Expression by Caveolin-1". ... Recent study has identified Caveolin-1 as a potential regulator of TLR5 expression. In contrast to the decreased TLR4 level in ...
PTGS2 has been shown to interact with caveolin 1. PTGS2 (COX-2) was discovered in 1991 by the Daniel Simmons laboratory[better ... "Colocalization and interaction of cyclooxygenase-2 with caveolin-1 in human fibroblasts". J. Biol. Chem. 276 (37): 34975-82. ... 88 (1-2): 24-30. doi:10.1016/j.lfs.2010.10.017. PMC 3046773. PMID 21035466. Wang D, Patel VV, Ricciotti E, Zhou R, Levin MD, ... PTGS1 (COX-1) is constitutively expressed in many tissues and is the predominant form in gastric mucosa and in the kidneys. ...
"A functional interaction between sprouty proteins and caveolin-1". The Journal of Biological Chemistry. 281 (39): 29201-12. doi ... Protein sprouty homolog 1 is a protein that in humans is encoded by the SPRY1 gene. Neurofibromin 1 SPRED1 GRCh38: Ensembl ... 342 (1-2): 57-62. doi:10.1007/s11010-010-0468-8. PMC 2923832. PMID 20461448. SPRY1 human gene location in the UCSC Genome ... "Entrez Gene: SPRY1 sprouty homolog 1, antagonist of FGF signaling (Drosophila)". Lim J, Wong ES, Ong SH, Yusoff P, Low BC, Guy ...
Cabrita MA, Jäggi F, Widjaja SP, Christofori G (Sep 2006). "A functional interaction between sprouty proteins and caveolin-1". ... 287 (1): L52-9. doi:10.1152/ajplung.00430.2003. PMID 14977631. Sasaki A, Taketomi T, Kato R, Saeki K, Nonami A, Sasaki M, ...
Cabrita MA, Jäggi F, Widjaja SP, Christofori G (2006). "A functional interaction between sprouty proteins and caveolin-1". J. ... 36 (1): 40-5. doi:10.1038/ng1285. PMID 14702039. Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and ...
Endothelin receptor type B has been shown to interact with Caveolin 1. Agonists IRL-1620 Antagonists A-192,621 BQ-788 Bosentan ... Yamaguchi T, Murata Y, Fujiyoshi Y, Doi T (Apr 2003). "Regulated interaction of endothelin B receptor with caveolin-1". ... 178 (1): 248-55. doi:10.1016/0006-291X(91)91806-N. PMID 1648908. Cyr C, Huebner K, Druck T, Kris R (Nov 1991). "Cloning and ... 181 (1): 184-90. doi:10.1016/S0006-291X(05)81399-3. PMID 1659806. "Entrez Gene: EDNRB endothelin receptor type B". Sato-Jin K, ...
Caveolin-1 conversely inhibits ROMK channel activity, and expression of the two shows a clear inverse relationship. Co- ... MicroRNA Lin DH, Yue P, Pan C, Sun P, Wang WH (2011). "MicroRNA 802 stimulates ROMK channels by suppressing caveolin-1". J Am ... miR-802 expression in the kidney is stimulated by a high potassium intake, along with caveolin-1 expression, one of the many ... Lin, D. -H.; Yue, P.; Pan, C.; Sun, P.; Wang, W. -H. (2011). "MicroRNA 802 Stimulates ROMK Channels by Suppressing Caveolin-1 ...
... directly binds caveolin-1". FEBS Letters. 508 (1): 49-52. doi:10.1016/S0014-5793(01)03020-4. PMID 11707266. S2CID 7887096. ... 8 (1): 157-71. doi:10.1074/mcp.M800266-MCP200. PMC 2621004. PMID 18782753. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S ... 51 (1): 136-9. doi:10.1006/geno.1998.5342. PMID 9693043. Castets F, Bartoli M, Barnier JV, Baillat G, Salin P, Moqrich A, ... 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. v t e (Genes on human chromosome 2, All stub articles, Human ...
"Ligand-independent activation of oestrogen receptor alpha by caveolin-1". The Biochemical Journal. 359 (Pt 1): 203-210. doi: ... 16 (1): 128-140. doi:10.1210/mend.16.1.0755. PMID 11773444. DiRenzo J, Shang Y, Phelan M, Sif S, Myers M, Kingston R, Brown M ( ... 1 (4): 237-250. PMC 376461. PMID 9222609. Lee MO, Kim EO, Kwon HJ, Kim YM, Kang HJ, Kang H, Lee JE (February 2002). "Radicicol ... 25 (1): 45-71. doi:10.1210/er.2003-0023. PMID 14769827. Anzick SL, Kononen J, Walker RL, Azorsa DO, Tanner MM, Guan XY, et al ...
... directly binds caveolin-1". FEBS Lett. 508 (1): 49-52. doi:10.1016/S0014-5793(01)03020-4. PMID 11707266. S2CID 7887096. Suzuki ... 8 (1): 157-71. doi:10.1074/mcp.M800266-MCP200. PMC 2621004. PMID 18782753. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S ... 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. Goudreault M, D'Ambrosio LM, Kean MJ, Mullin MJ, Larsen BG, ... 200 (1-2): 149-56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. Maruyama K, Sugano S (1994). "Oligo-capping: a simple ...
2007). "The tetraspan protein EMP2 regulates expression of caveolin-1". J. Biol. Chem. 282 (36): 26542-51. doi:10.1074/jbc. ... 183 (1-2): 69-75. doi:10.1016/S0378-1119(96)00475-1. PMID 8996089. Liehr T, Kuhlenbaumer G, Wulf P, Taylor V, Suter U, Van ... 58 (1): 106-8. doi:10.1006/geno.1999.5803. PMID 10331954. Wadehra M, Forbes A, Pushkarna N, Goodglick L, Gordon LK, Williams CJ ... 175 (1-2): 115-20. doi:10.1016/0378-1119(96)00134-5. PMID 8917086. Street VA, Goldy JD, Golden AS, et al. (2002). "Mapping of ...
"Functional interaction of caveolin-1 with Bruton's tyrosine kinase and Bmx". J. Biol. Chem. 277 (11): 9351-7. doi:10.1074/jbc. ... Phase 1: ONO-4059 for non-Hodgkin lymphoma and/or CLL. Renamed GS-4059 and now in trial NCT02457598. Spebrutinib (AVL-292, CC- ... 228 (1): 42-53. doi:10.1016/j.cellimm.2004.03.004. PMID 15203319. Yasuda T, Tezuka T, Maeda A, Inazu T, Yamanashi Y, Gu H, ... 461 (1-2): 68-72. doi:10.1016/S0014-5793(99)01424-6. PMID 10561498. Matsushita M, Yamadori T, Kato S, Takemoto Y, Inazawa J, ...
High levels of caveolin Cav1 are expressed in adipocytes. Caveolin associates with cholesterol, fatty acids and lipid droplets ... The presence of caveolin leads to a local change in morphology of the membrane. Cavin proteins emerged in the late 2000s to be ... Formation and maintenance of caveolae was initially thought to be primarily due to caveolin, a 21 kD protein. There are three ... However, some bacteria do not use typical caveolae but only caveolin-rich areas of the plasma membrane. The pathogens ...
"Interaction of the immunoglobulin-like cell adhesion molecule hepaCAM with caveolin-1". Biochemical and Biophysical Research ... 37 (1): 155-65. doi:10.3892/ijo_00000663. PMID 20514407. Moh MC, Shen S (2009). "The roles of cell adhesion molecules in tumor ... In cell signaling, hepaCAM directly interacts with F-actin and calveolin 1, and is capable of inducing senescence-like growth ...
Thus, microRNA-103/107 may affect insulin sensitivity by targeting caveolin-1. The blockmir CD5-2 has been shown to inhibit the ... MicroRNA-103/107 inhibition in caveolin-1-deficient mice had no effect on insulin sensitivity and signalling. ... 107 inhibition in mice leads to increased insulin sensitivity and signalling It has been previously shown that caveolin-1- ... This may be achieved by designing a Blockmir that matches seed 1.[citation needed] MicroRNA-33a/b inhibition in mice leads to ...
June 2009). "Stromal caveolin-1 levels predict early DCIS progression to invasive breast cancer". Cancer Biology & Therapy. 8 ( ... 185): 1-549. PMC 4781639. PMID 20629475. Quinn CM, D'Arcy C, Wells C (January 2022). "Apocrine lesions of the breast". Virchows ... 12 (1): 21-9. doi:10.1016/S1470-2045(10)70266-7. PMC 3018565. PMID 21145284. Ductal Carcinoma in Situ (DCIS), Johns Hopkins ... 480 (1): 177-189. doi:10.1007/s00428-021-03185-4. PMC 8983539. PMID 34537861. Raphael Rubin; David S. Strayer, eds. (2008). ...
Caveolin 1 has been shown to interact with Nitric oxide synthase 2A. and Rac2. Autosomal recessive NOS2 deficiency has been ... Felley-Bosco E, Bender FC, Courjault-Gautier F, Bron C, Quest AF (December 2000). "Caveolin-1 down-regulates inducible nitric ... occurring in fewer than 1 per million persons. GRCh38: Ensembl release 89: ENSG00000007171 - Ensembl, May 2017 GRCm38: Ensembl ...
March 1999). "Phospholipase D1 in caveolae: regulation by protein kinase Calpha and caveolin-1". Biochemistry. 38 (12): 3763-9 ... 302 (1): 127-32. doi:10.1016/s0006-291x(03)00112-8. PMID 12593858. Cai S, Exton JH (May 2001). "Determination of interaction ... 168 (1): 233-40. doi:10.1016/S0021-9258(17)35110-4. PMID 20291081. Jenkins GM, Frohman MA (October 2005). "Phospholipase D: a ... July 1998). "Activation of phospholipase D1 by direct interaction with ADP-ribosylation factor 1 and RalA". FEBS Letters. 430 ( ...
2002). "Functional interaction of caveolin-1 with Bruton's tyrosine kinase and Bmx". J. Biol. Chem. 277 (11): 9351-7. doi: ... doi:10.1016/S1570-9639(02)00524-1. PMID 12573241. Zhang R, Xu Y, Ekman N, et al. (2004). "Etk/Bmx transactivates vascular ...
Caveolin-3 is one of three isoforms of the protein caveolin. Caveolin-3 is concentrated in the caveolae of myocytes, and ... Knockout of caveolin-3 genes are sufficient to induce these manifestations. Similarly, dominant-negative genotypes for caveolin ... Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting ... There are many proteins that associate with caveolin-3, including ion channels and exchangers. In cardiac myocytes, caveolin-3 ...
... has been shown to interact with Caveolin 1 and peroxisomal receptor PEX5. GRCh38: Ensembl release 89: ENSG00000116171 - ... "Sterol carrier protein-2 directly interacts with caveolin-1 in vitro and in vivo". Biochemistry. 43 (23): 7288-306. doi:10.1021 ... 76 (1): 73-84. doi:10.1016/0009-3084(95)02436-M. PMID 7788802. Vesa J, Hellsten E, Barnoski BL, et al. (1994). "Assignment of ... 335 (1): 18-26. doi:10.1016/0014-5793(93)80431-S. PMID 8243660. S2CID 9969358. Seedorf U, Scheek S, Engel T, et al. (1994). " ...
This gene codes for the Caveolin protein, which is a scaffolding membrane protein. This protein plays a role in lipid ... April 2008). "Association of a Homozygous Nonsense Caveolin-1 Mutation with Berardinelli-Seip Congenital Lipodystrophy". ... In type 1 patients, they still have mechanical adipose tissue, but type 2 patients do not have any adipose tissue, including ... The gene for type 1 CGL was identified as AGPAT2 at chromosome 9q34, and later the gene for type 2 CGL was identified as BSCL2 ...
Caveolin 1 is dephosphorylated on tyrosine 14 in response to shear stress and PTPmu is hypothesized to catalyze this reaction. ... Caveolin 1 is a scaffolding protein enriched in endothelial cell junctions that is also linked to shear stress regulated ... Shin J, Jo H, Park H (2006). "Caveolin-1 is transiently dephosphorylated by shear stress-activated protein tyrosine phosphatase ... 248 (1): 329-38. doi:10.1006/excr.1999.4428. PMID 10094839. Koop EA, Lopes SM, Feiken E, Bluyssen HA, van der Valk M, Voest EE ...
Caveolin 1, Tight junction protein 1 CSNK1D, and PTPmu (PTPRM). Connexin Hypoplastic left heart syndrome GRCh38: Ensembl ... "Connexin family members target to lipid raft domains and interact with caveolin-1". Biochemistry. 41 (18): 5754-5764. doi: ... Gap junction alpha-1 protein (GJA1), also known as connexin 43 (Cx43), is a protein that in humans is encoded by the GJA1 gene ... 58 (1): 34-40. doi:10.1006/geno.1999.5814. PMID 10331943. Fishman GI, Eddy RL, Shows TB, Rosenthal L, Leinwand LA (May 1991). " ...
Clathrin and Caveolin 2-Dependent Manner". Cellular Microbiology. 11 (4): 629-644. doi:10.1111/j.1462-5822.2008.01279.x. PMC ... In general, about 1-3% of the tick population carries R. rickettsii, even in areas where the majority of human cases are ... 65 (2): 1-44. doi:10.15585/mmwr.rr6502a1. ISSN 1057-5987. PMID 27172113. Saraiva DG, Soares HS, Soares JF, Labruna MB (2014). " ... 55 (1): 42-50. doi:10.1016/j.jemermed.2018.02.043. ISSN 0736-4679. PMID 29685474. S2CID 23489868. CDC (2019-02-19). "Signs and ...
Llorente A, de Marco MC, Alonso MA (Oct 2004). "Caveolin-1 and MAL are located on prostasomes secreted by the prostate cancer ... Millán J, Puertollano R, Fan L, Alonso MA (Apr 1997). "Caveolin and MAL, two protein components of internal detergent-insoluble ...
Llorente A, de Marco MC, Alonso MA (2005). "Caveolin-1 and MAL are located on prostasomes secreted by the prostate cancer PC-3 ... 7 (1): 61-73. doi:10.1111/j.1600-0854.2005.00361.x. PMID 16445687. S2CID 44752098. Rual JF, Venkatesan K, Hao T, et al. (2005 ... 36 (1): 40-5. doi:10.1038/ng1285. PMID 14702039. Marazuela M, Martín-Belmonte F, García-López MA, et al. (2004). "Expression ... 76 (1-3): 81-8. doi:10.1006/geno.2001.6610. PMID 11549320. "Entrez Gene: MAL2 mal, T-cell differentiation protein 2". Marazuela ...
... with the help of adaptor proteins such as caveolin 1 or SHC1, which target the ER complexes to the plasma membrane. Activation ... 12 (1): 10-9. doi:10.1097/00125480-200501000-00003. PMID 15614160. Lledge, R. M. E.; Reen, S. G.; Ugh, R. P.; Llred, D. C. A.; ... Both of these are nuclear receptors activated by the sex hormone, estrogen, and encoded by the ESR1 (Estrogen Receptor 1) gene ... Deroo, B. J.; Korach, K. S. (1 March 2006). "Estrogen receptors and human disease". Journal of Clinical Investigation. 116 (3 ...
"The interaction between caveolin-1 and Rho-GTPases promotes metastasis by controlling the expression of alpha5-integrin and the ... 1(7): p. 486-503). {{cite journal}}: Cite journal requires ,journal= (help) Li P, Zhou H, Zhu X, Ma G, Liu C, Lin B, Mao W ( ... 119 (Pt 1): 96-103. doi:10.1242/jcs.02712. PMID 16352661. Bradbury P, Mahmassani M, Zhong J, Turner K, Paul A, Verrills NM, ... 386 (1): 190-203. doi:10.1016/j.jmb.2008.12.010. PMID 19103205. Bradshaw LN, Zhong J, Bradbury P, Mahmassani M, Smith JL, Ammit ...
... has been shown to interact with Caveolin 3 in skeletal muscle, and this interaction is thought to retain dysferlin ... Hernández-Deviez DJ, Howes MT, Laval SH, Bushby K, Hancock JF, Parton RG (2008). "Caveolin regulates endocytosis of the muscle ... Dysferlin also interacts with MG53, and a functional interaction between dysferlin, caveolin-3 and MG53 is thought to be ... caveolin-3, and dysferlin". J. Biol. Chem. 284 (23): 15894-902. doi:10.1074/jbc.M109.009589. PMC 2708885. PMID 19380584. ...
This region also contains a caveolin binding domain (CBD). The C-terminal end of the channel extends into a loop (residues 47- ... residues 1-23); (ii) a transmembrane segment (TMS) (residues 24-46); (iii) an intracellular C-terminal domain (residues 47-97 ...
LQT9 is caused by variants in the membrane structural protein, caveolin-3. Caveolins form specific membrane domains called ... Similar to LQT3, these caveolin variants increase the late sustained sodium current, which impairs cellular repolarization. ... "Long QT syndrome 1 , Genetic and Rare Diseases Information Center (GARD) - an NCATS Program". rarediseases.info.nih.gov. ... 61 (1): 51-55. doi:10.1038/jhg.2015.74. PMID 26108145. S2CID 30987284. (Articles with short description, Short description is ...
2005). "Junctophilin type 2 is associated with caveolin-3 and is down-regulated in the hypertrophic and dilated ... 6 (1): 11-22. doi:10.1016/S1097-2765(05)00005-5. PMID 10949023. Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA ... 2 (1): 47-53. doi:10.1038/nmeth726. PMID 15782160. S2CID 6135437. Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo ... 6 (1): 11-22. doi:10.1016/S1097-2765(05)00005-5. PMID 10949023. Nishi M, Mizushima A, Nakagawara K, Takeshima H (July 2000). " ...
... cell carcinomas and nonurothelial carcinomas of the urinary bladder differ in the promoter methylation status of the caveolin-1 ... 36 (1): 40-5. doi:10.1038/ng1285. PMID 14702039. von Bergh AR, Wijers PM, Groot AJ, et al. (2004). "Identification of a novel ... 17 (1): 3-13. doi:10.3892/ijmm.17.1.3. PMID 16328005. Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based ... 113 (1): 59-66. doi:10.1002/ijc.20531. PMID 15386433. S2CID 24207780. Chen H, Tu SW, Hsieh JT (2005). "Down-regulation of human ...
1998). "T-cadherin and signal-transducing molecules co-localize in caveolin-rich membrane domains of vascular smooth muscle ... 463 (1-2): 29-34. doi:10.1016/S0014-5793(99)01594-X. PMID 10601632. S2CID 5950399. Takeuchi T, Misaki A, Liang SB, et al. (2000 ... 103 (1): 96-101. doi:10.1007/s004390050790. PMID 9737784. S2CID 30812156. Sato M, Mori Y, Sakurada A, et al. (1999). "A GT ... LDL binding to T-cadherin leads to the activation of Erk 1/2 tyrosine kinase and the nuclear translocation of NF-kappaB. T- ...
The C-terminus of DLC1 is also known to interact with caveolin-1, although the biological significance of this interaction has ... Mar 2005). "Identification of ATF-3, caveolin-1, DLC-1, and NM23-H2 as putative antitumorigenic, progesterone-regulated genes ... 176 (1): 43-9. doi:10.1083/jcb.200608015. PMC 2063623. PMID 17190795. Kim TY, Lee JW, Kim HP, et al. (Mar 2007). "DLC-1, a ... 355 (1): 72-7. doi:10.1016/j.bbrc.2007.01.121. PMID 17292327. Durkin ME, Avner MR, Huh CG, Yuan BZ, Thorgeirsson SS, Popescu NC ...
... has been shown to interact with: CRK, Caveolin 1, Grb2, NCK1, NCK2, PDGFR-α, PTPN11, RAS p21 protein activator 1, SHC1 ... Yamamoto M, Toya Y, Jensen RA, Ishikawa Y (March 1999). "Caveolin is an inhibitor of platelet-derived growth factor receptor ... 34 (1): 110-22. doi:10.1128/MCB.00839-13. PMC 3911282. PMID 24190966. Lei H, Qian CX, Lei J, Haddock LJ, Mukai S, Kazlauskas A ... 225 (1): 29-41. doi:10.1111/j.1432-1033.1994.00029.x. PMID 7523122. Seifert RA, Hart CE, Phillips PE, Forstrom JW, Ross R, ...
... cell by binding to receptors on the surface of the cell and entering it by endocytosis mediated by either clathrin or caveolin- ... 38 (1-2): 24-34. doi:10.1159/000150411. PMID 8666521. Ghosh S, Banerjee P, Deny P, Mondal RK, Nandi M, Roychoudhury A, et al. ( ... 64 (1): 51-68. doi:10.1128/mmbr.64.1.51-68.2000. PMC 98986. PMID 10704474. Lin YJ, Wu HL, Chen DS, Chen PJ (September 2012). " ... 8: 1-9. doi:10.1016/j.coviro.2014.04.005. PMID 24814823. Yang HC, Kao JH (September 2014). "Persistence of hepatitis B virus ...
Caveolin 1 and caveolin 2 are overexpressed, and may contribute to tumour cell motility E-cadherin is overexpressed; ... "Overexpression of caveolin-1 and -2 in cell lines and in human samples of inflammatory breast cancer" (PDF). Breast Cancer ... of caveolin 1 and caveolin 2. Caveolin is, paradoxically, tumour-promoting in IBC. NF-κB pathway activation overexpression may ... IBC is typically diagnosed in one of these stages: Stage IIIB - at least 1/3 of the skin of the breast is affected, and cancer ...
Razani B, Lisanti MP (2001). "Two distinct caveolin-1 domains mediate the functional interaction of caveolin-1 with protein ... 2001). "AMY-1, a c-Myc-binding protein, is localized in the mitochondria of sperm by association with S-AKAP84, an anchor ... 2003). "AAT-1, a novel testis-specific AMY-1-binding protein, forms a quaternary complex with AMY-1, A-kinase anchor protein 84 ... 225 (1): 313-9. doi:10.1006/bbrc.1996.1172. PMID 8769136. Lin RY, Moss SB, Rubin CS (Jan 1996). "Characterization of S-AKAP84, ...
Hyaluronan is tethered to cell surfaces by CD44 which are anchored in caveolin-rich lipid rafts. Cleavage and further ... Regulation of PFK-1 is also mediated by the cellular energy status in result of ATP's inhibitory effect. Greater quantities of ... HIF-1 activated pyruvate kinase M comes in multiple isoforms known as PKM1 and PKM2. Pyruvate kinase is shown to convert ... Enolase 1, also known as α-enolase, is encoded by the ENOA gene and is responsible for converting 2-phosphoglycerate to ...
Wary, K.K.; Mariotti, A.; Zurzolo, C.; Giancotti, F.G. (1998). "A requirement for caveolin-1 and associated kinase Fyn in ... Wickstrom, S.A.; Alitalo, K.; Keski-Oja, J. (2002). "Endostatin associates with integrin alpha5beta1 and caveolin-1, and ... Endostatin binding and clustering of integrins causes co-localization with caveolin-1 and activates non-receptor tyrosine ... 5 (5 Pt 1): 547-54. doi:10.1006/mthe.2002.0590. PMID 11991745. Dhanabal, M.; Volk, R.; Ramchandran, R.; Simons, M.; Sukhatme, V ...
Carman CV, Lisanti MP, Benovic JL (Mar 1999). "Regulation of G protein-coupled receptor kinases by caveolin". Journal of ... 521 (1-3): 140-4. doi:10.1016/S0014-5793(02)02858-2. PMID 12067742. S2CID 29467488. Portal: Biology v t e (Articles with short ... 275 (1): 390-7. doi:10.1074/jbc.275.1.390. PMID 10617630. Pronin AN, Morris AJ, Surguchov A, Benovic JL (Aug 2000). "Synucleins ... Hu LA, Chen W, Premont RT, Cong M, Lefkowitz RJ (Jan 2002). "G protein-coupled receptor kinase 5 regulates beta 1-adrenergic ...
... has been shown to interact with: ARF1, Aldolase A, Amphiphysin, BIN1, Caveolin 1, Glyceraldehyde 3-phosphate dehydrogenase ... Zheng X, Bollinger Bollag W (December 2003). "Aquaporin 3 colocates with phospholipase d2 in caveolin-rich membrane ... functional interaction with caveolin in low-density membrane microdomains". FEBS Letters. 467 (2-3): 326-32. doi:10.1016/s0014- ... functional interaction with caveolin in low-density membrane microdomains". FEBS Letters. 467 (2-3): 326-32. doi:10.1016/S0014- ...
2009). "Caveolin-1 scaffold domain interacts with TRPC1 and IP3R3 to regulate Ca2+ store release-induced Ca2+ entry in ... Inositol 1,4,5-trisphosphate receptor, type 3, also known as ITPR3, is a protein which in humans is encoded by the ITPR3 gene. ... "Entrez Gene: inositol 1". Yamamoto-Hino M, Sugiyama T, Hikichi K, et al. (1994). "Cloning and characterization of human type 2 ... 2 (1): 9-22. PMID 8081734. Chaudhari N, Roper SD (August 2010). "The cell biology of taste". J. Cell Biol. 190 (3): 285-96. doi ...
High amounts of GM3 also displayed a high amount of caveolin-1, a molecule which has been shown to inhibit ovarian cancer ... 81 (1): 210.e11-5. doi:10.1016/j.urology.2012.08.015. PMID 23102779. v t e (Wikipedia articles needing context from August 2014 ...
... caveolin, dynamin and actin. Once within the cell the viruses are rapidly delivered to endosomes via vesicular trafficking ... 1. SAGE. p. 354. ISBN 978-1-4129-4186-0. OCLC 775277696. Buckley SM, Casals J, Downs WG (July 1970). "Isolation and antigenic ... 57 (1-2): 61-87. doi:10.1016/s0166-3542(02)00201-2. PMID 12615304. Yun NE, Walker DH (October 2012). "Pathogenesis of Lassa ... It has been reported that the cellular protease SKI-1/S1P is responsible for this cleavage. The cleaved glycoproteins are ...
eNOS localizes to caveolae, a plasma membrane domain primarily composed of the protein caveolin 1, and to the Golgi apparatus. ... 84 (1): 54-61. doi:10.1016/j.diff.2012.04.004. PMID 22579300. Burger DE, Lu X, Lei M, Xiang FL, Hammoud L, Jiang M, Wang H, ... 43 (1-2): 87-94. doi:10.1016/0165-2478(94)00158-8. hdl:2027.42/31140. PMID 7537721. Mungrue IN, Husain M, Stewart DJ (October ... 52 (1): 80-89. doi:10.1017/S0033291720001749. PMID 32524920. S2CID 219587961. Retrieved 26 December 2021. Taylor BS, Kim YM, ...
Among the caveolin family, caveolin-1 and -3 are mainly expressed in endothelial and muscle cells, respectively. In this study ... Total abundance of caveolin-1 and -3 remained stable whatever the group. In the fractions free of caveolae (Triton X-100 ... NOS3/caveolin-1 interactions were evaluated by immunoprecipitation assays. RESULTS: At the microscope level, caveolin-1 ... Conversely the typical distribution of caveolin-3 in myocyte sarcolemma was dramatically altered in S-MI rats, resulting in a ...
This study hypothesizes that caveolae and Caveolin-1 are important for the effects of 1,25 Vitamin D signaling via ERp60. ... Histology and transmission electron microscopy were also used to examine the physiological importance of caveolae and Caveolin- ... Caveolae and its characteristic protein Caveolin-1 have been involved in many signaling pathways due to its specific structure ... This study hypothesizes that caveolae and Caveolin-1 are important for the effects of 1,25 Vitamin D signaling via ERp60. ...
Caveolin-1 (CAV1) is a membrane-sculpting protein that oligomerizes to generate flask-shaped invaginations of the plasma ... Structural analysis of the P132L disease mutation in caveolin-1 reveals its role in the assembly of oligomeric complexes. ... cancer; caveolae; caveolin; computational biology; intracellular trafficking; membrane protein; oligomerization; structural ... Structural analysis of the P132L disease mutation in caveolin-1 reveals its role in the as ...
CAV3: caveolin 3. *CAVIN1: caveolae associated protein 1. *CBFB: core-binding factor subunit beta ...
Role of caveolin and heat shock protein 70 interaction in the antioxidative effects of an angiotensin II type 1 receptor ... Caveolin-1 and Hsp70 interaction in microdissected proximal tubules from spontaneously hypertensive rats as an effect of ... January 2010 - Volume 28 - Issue 1 : Journal of Hypertension. You may be trying to access this site from a secured browser on ...
Role of Caveolin-1 in carbon nanotube-induced stem-like cells and tumorigenesis. ... Overexpression of Cav-1 further promoted the CSC induction, cell motility, and in vivo tumorigenesis. Our findings provide ... These CSC-like cells, which express a high level of Cav-1, displayed increased cell migration and invasion, compared with their ... and demonstrated a positive regulatory role of Cav-1. We suggest that detection of CSC may provide a valuable tool for early ...
Name: caveolin 1, caveolae protein. Synonyms: Cav-1, caveolin-1. Type: Gene ... 1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The ... Name: protein tyrosine phosphatase receptor type Z, polypeptide 1. Synonyms: DSD-1-PG, phosphacan, PTPzeta, PTPbeta, Rptpbeta, ... Synonyms: P400, IP3R1, Itpr-1, Pcp-1, Ip3r, opt, InsP3R type I, Pcp1, wblo ...
Antibodies for proteins involved in cellular response to starvation pathways, according to their Panther/Gene Ontology Classification
2011). Role of caveolin-1 in the pathogenesis of tissue fibrosis by keloid-derived fibroblasts in vitro. Br. J. Dermatol. 164, ... Caveolin 1 is a component of membrane caveolae that is proposed to regulate TGF-β receptor degradation (Del Galdo et al., 2008a ... 2012). Evidence for caveolin-1 as a new susceptibility gene regulating tissue fibrosis in systemic sclerosis. Ann. Rheum. Dis. ... 2006). Caveolin-1: a critical regulator of lung fibrosis in idiopathic pulmonary fibrosis. J. Exp. Med. 203, 2895-2906. doi: ...
Replication Study: Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis ... 2017 Jan;150(1):25-34. doi: 10.1111/imm.12664. Epub 2016 Sep 20. Immunology. 2017. PMID: 27564847 Free PMC article. Review. ... 2018 Aug 6;6(1):78. doi: 10.1186/s40425-018-0381-3. J Immunother Cancer. 2018. PMID: 30081947 Free PMC article. Review. ... Among authors: fiering s. Cancers (Basel). 2022 Dec 23;15(1):82. doi: 10.3390/cancers15010082. Cancers (Basel). 2022. PMID: ...
26046 Caveolin-1 as a prime modulator of aging? [Doug Skrecky] (8739 Bytes) *#26047 Cryonic contracts [marta sandberg] (1977 ... 26096 Uploading (3.ii.1) First bits. [Azt28] (3416 Bytes) *#26097 Re: CryoNet #26084 other countries [Azt28] (1532 Bytes) *# ... 26089 Uploading technology (1.i.0) Electronics next generation. [Azt28] (2986 Bytes) *#26090 To Francious and Yvan, re ...
Chen, Hung-Chih (2014). Functions of Caveolin-1 and Caveolin-3 in muscular dystrophy. University of Birmingham. Ph.D. ... Hogan, Julia Michelle (2014). Davids Women: A critical comparison of Michal, Bathsheba and Tamar in 1 Samuel and 2 Samuel. ... Wardzinski, Lukasz (2014). Control of T cell interleukin 21 production in a mouse model of type-1 diabetes. University of ... Edginton-White, Benjamin (2014). Role of cap 1 and cap 2 2ʼ-O-ribose methylation in Drosophila development and nervous system ...
Thus, ACE2 and caveolin-1/NF-B signaling may be associated with the mechanism of the protective effect of GA in LPS-induced ALI ... GA activated the ACE2 and caveolin-1 pathways, alleviating LPS-induced ALI and providing a novel prospect of GA-ameliorated ALI ... and Caveolin-1 signaling pathway. Inflammation, 45, 253-266. [Google Scholar] [PubMed] [CrossRef] ... GA could suppress caveolin-1/NF-B expression by activating ACE2. ... 1, Suppl. Tables S1-S4).. Some Indian Medicinal Plants with ...
Basal-like tumors can be characterized by cytokeratin 5, 17, caveolin 1 and 2, nestin, CD44 and EGFR expression and have the ... Figure 1. Reproducibility of the molecular subtypes in three different approaches. Author: as published in GEO by the authors ... Figure 1. Reproducibility of the molecular subtypes in three different approaches. Author: as published in GEO by the authors ... For the generation of Figure 1, we used established cutoffs for ESR1 and HER2 which have been extensively validated [29,40]. ...
C, E, The number of α-synuclein puncta colocalized with caveolin-1 (C) and cathepsin D (E) was quantified. Three thousand ... they hardly colocalized with caveolin-1 (Fig. 6B,C). This is consistent with the finding that the 274 antibody alters the ... and caveolin-1 monoclonal antibody (BD Biosciences, #C13620). FITC-labeled cholera toxin B subunit (CTB) was purchased from ... of internalized α-synuclein aggregates were found in the compartments positive for caveolin-1, whereas in the presence of ...
Glucocorticoid-mediated induction of caveolin-1 disrupts cytoskeletal organization, inhibits cell migration and re- ... Figure 1. Cdc42 activation by EGF measured by the Cdc42 G-LISA Activation Assay. Swiss 3T3 cells were serum starved (SS) for 16 ... Answer 1: The Rac1 and RhoA G-LISAs use the same lysis buffer (Part # GL36). The Cdc42 G-LISA kit (Cat. # BK127) uses a ... Question 1: Can I use the lysis buffer from the Cdc42 G-LISA activation assay kit (Cat. # BK127) to prepare samples for the ...
Caveolin-1 facilitates cell migration by upregulating nuclear receptor 4A2/retinoid X receptor alpha-mediated beta-galactoside ... Identification of a GDP-Fuc:Gal beta 1-3GalNAc-R (Fuc to Gal) alpha 1-2 fucosyltransferase and a GDP-Fuc:Gal beta 1-4GlcNAc ( ... Biosynthesis of intestinal glycoprotein: a study of an (1 lead to 2) fucosyltransferase in rat small intestinal mucosa ... Changing transcription start sites in H-type alpha(1,2)fucosyltransferase gene (FUT1) during differentiation of the human ...
Caveolin-assisted sphingolipid transport to the plasma membrane. Lipid and protein sorting are crucial processes that maintain ... The goal of this project is to determine the role of caveolin in SM trafficking from the TGN to the PM and to decipher the ... One of the proteins enriched in the same secretory pathway is caveolin, which shuttles between the Golgi and the PM where it ... The assembly process is initiated by the export of caveolin-enriched vesicles from the Golgi but little is known about this ...
Wickstrom S, Alitalo K, Keski-Oja J. Endostatin associates with integrin alpha5beta1 and caveolin-1, and activates Src via a ... EMHJ, 2006, 12(1-2): 178-187 Introduction. Pre-eclampsia is a multi-system disorder peculiar to human pregnancy and characte- ... Table 1 shows mean age, gestational age at sampling and clinical characteristics of the study groups, e.g. blood pressure and ... R.A.K. Mahmoud1 and M. Abdel Raouf2. ABSTRACT We evaluated the prognostic value of serum endostatin and vascular endothelial ...
Wickstrom S, Alitalo K, Keski-Oja J. Endostatin associates with integrin alpha5beta1 and caveolin-1, and activates Src via a ... EMHJ, 2006, 12(1-2): 178-187 Introduction. Pre-eclampsia is a multi-system disorder peculiar to human pregnancy and characte- ... Table 1 shows mean age, gestational age at sampling and clinical characteristics of the study groups, e.g. blood pressure and ... R.A.K. Mahmoud1 and M. Abdel Raouf2. ABSTRACT We evaluated the prognostic value of serum endostatin and vascular endothelial ...
Caveolin-1 expression and GM1 transcytosis were also reduced, yet increased GM1 levels seemed to compensate, providing similar ... JTD Keywords: 1-deoxy-d-xylulose-5-phosphate reductoisomerase, Apicoplast, Dna aptamers, Drug targets, Evolution, Inhibitors, ... JTD Keywords: Malaria, 2-C-methyl-D-erythritol-4-phosphate pathway, 1-deoxy-D-xylulose 5-phosphate synthase, Pyruvate, ... JTD Keywords: acid sphingomyelinase, antibody-affinity, blood -brain barrier, drug-delivery, icam-1-targeted nanocarriers, in- ...
Caveolin-1; K-M: Kaplan-Meier; GEO: Gene Expression Omnibus; ROC: Receiver operating characteristic curve; DCA: Decision curve ... Yichen Wang1, , Wenchang Lv1, , Yi Yi1, , Qi Zhang1, , Jun Zhang2, &, , Yiping Wu1, , * 1 Department of Plastic Surgery, Tongji ...
Abstract This study evaluated the effect of the volatile oil of Alpinia zerumbet (VOAz) on caveolin-1 gene expression and ... Animals , Rats , Oils, Volatile/pharmacology , Collagen/metabolism , Alpinia/chemistry , Caveolin 1/metabolism , Muscles/drug ... Collagen quality was assessed by histology and the expression of the caveolin-1 (CAV-1) gene was evaluated using qPCR. ... Chemical characterization and effects of volatile oil of Alpinia zerumbet on the quality of collagen deposition and caveolin-1 ...
Caveolin internalization by heat shock or hyperosmotic shock. Kang, Y. S., Ko, Y. G. & Seo, J. S., 2000 Mar 15, In: ... Caveolin-1 deficiency induces premature senescence with mitochondrial dysfunction. Yu, D. M., Jung, S. H., An, H. T., Lee, S., ... Caveolin-2 regulation of STAT3 transcriptional activation in response to insulin. Kwon, H., Jeong, K., Hwang, E. M., Park, J. Y ... Caveolin-1 is associated with VCAM-1 dependent adhesion of gastric cancer cells to endothelial cells. Shin, J., Kim, J., Ryu, B ...
Vascular microarray profiling in two models of hypertension identifies caveolin-1, Rgs2 and Rgs5 as antihypertensive targets. ... Table 1 Genes differentially expressed in ISIAH and WAG brain stems and referred to in Databases as associated with blood ... Additional file 1:. Table S1. Genes differentially expressed in brain stems of 3-month old hypertensive ISIAH and normotensive ... cDNA synthesis was performed using the following protocol: 1 h at 37 °C, 30 min at 42 °C, 10 min at 50 °C, and 5 min at 75 °C ...
... caveolin-1) in 3T3-L1 adipocytes resulted in down-regulation of expression of membrane-associated PDE3B. Insulin-induced ... effects of caveolin-1 knockdown on formation/maintenance of macromolecular signalling complexes Author ... effects of caveolin-1 knockdown on formation/maintenance of macromolecular signalling complexes ... Similar results were obtained in adipocytes from Cav-1-deficient mice. siRNA-mediated KID of CAV-1 in 3T3-L1 adipocytes also ...
Caveolin-1 inhibits proliferation and migration of gastric cancer cell via inactivating BMI-1 ... Repairing and Protecting Effects of Pneumatophorus japonicus heads peptides on GES-1 Cells Damaged by Alcohol. ...
Pro-atherosclerotic disturbed flow disrupts caveolin-1 expression, localization, and function via glycocalyx degradation. ... 08Pro-atherosclerotic disturbed flow disrupts caveolin-1 expression, localization, and function via glycocalyx degradation. ... June 1, 2019. /0 Comments/in Research /by Academic Web Pages. Over the past several decades, it has been shown that the ... Pro-atherosclerotic disturbed flow disrupts caveolin-1 expression, localization, and function via glycocalyx degradation ...
alpha Caveolin. alpha-Caveolin. beta Caveolin. beta-Caveolin. Tree number(s):. D12.644.360.024.264. D12.776.157.057.010. ... Caveolin 1 - Preferred Concept UI. M0209023. Scope note. A tyrosine phosphoprotein that plays an essential role in CAVEOLAE ... 2006; CAVEOLIN 1 was indexed under CAVEOLINS 2001-2005, & under MEMBRANE PROTEINS 1992-2000. ... Caveolin-1. VIP21 Protein. Vesicular Integral Membrane Protein 21 kDa. ...
Syndecan-2 exerts antifibrotic effects by promoting caveolin-1-mediated transforming growth factor-b receptor i internalization ... Haspel, J., Bauer, K., Goehler, A. & Roberts, D. H., May 1 2009, In: CHEST. 135, 5, p. 1243-1251 9 p.. Research output: ... Knauert, M. P., Haspel, J. A. & Pisani, M. A., Sep 1 2015, In: Clinics in Chest Medicine. 36, 3, p. 419-429 11 p.. Research ... Haspel, J., Blanco, C., Jacob, J. & Grumet, M., 2001, In: BioTechniques. 30, 1, p. 60-66 7 p.. Research output: Contribution to ...
  • Caveolin-1 (CAV1) is a membrane -sculpting protein that oligomerizes to generate flask-shaped invaginations of the plasma membrane known as caveolae . (bvsalud.org)
  • Significant association of caveolin-1 (CAV1) genotypes with prostate cancer susceptibility in Taiwan. (cdc.gov)
  • Significant association of caveolin-1 (CAV1) genotypes with upper urothelial tract cancer. (cdc.gov)
  • Caveolin-1 and -3 dissociations from caveolae to cytosol in the heart during aging and after myocardial infarction in rat. (inserm.fr)
  • Caveolae and its characteristic protein Caveolin-1 have been involved in many signaling pathways due to its specific structure and physical configuration. (gatech.edu)
  • This study hypothesizes that caveolae and Caveolin-1 are important for the effects of 1,25 Vitamin D signaling via ERp60. (gatech.edu)
  • Histology and transmission electron microscopy were also used to examine the physiological importance of caveolae and Caveolin-1 in growth plate morphology and cellular characteristics. (gatech.edu)
  • Insulin- and CL-induced macromolecular complexes were enriched in cholesterol, and contained certain common signalling proteins [14-3-3, PP2A (protein phosphatase 2A) and cav-1]. (lu.se)
  • The complexes present in insulin-stimulated cells contained tyrosine-phosphorylated IRS-1 (insulin receptor substrate 1) and its downstream signalling proteins, whereas CL-activated complexes contained beta(3)-adrenergic receptor, PKA-RII [PKA (cAMP-dependent protein kinase)-regulatory subunit] and HSL. (lu.se)
  • Among the caveolin family, caveolin-1 and -3 are mainly expressed in endothelial and muscle cells, respectively. (inserm.fr)
  • RESULTS: At the microscope level, caveolin-1 distribution in the endothelial cells was unchanged between the groups. (inserm.fr)
  • In endothelial dysfunction, there is reduction in procoagulatory milieu that favours all stages of the bio-availability of vasodilators, in particular, atherogenesis ( Figure 1 ) 6 . (who.int)
  • Genetic analysis of caveolin-1 and eNOS genes in colorectal cancer. (cdc.gov)
  • Resumo Este estudo avaliou o efeito do óleo volátil de Alpinia zerumbet (OVAz) na expressão do gene da caveolina-1 e na fibrose muscular. (bvsalud.org)
  • A qualidade do colágeno foi avaliada com histologia e à expressão do gene caveolina-1 (CAV-1) foi avaliada usando qPCR. (bvsalud.org)
  • NOS3/caveolin-1 interactions were evaluated by immunoprecipitation assays. (inserm.fr)
  • It remains a leading cause of maternal and neonatal mortality and morbidity [1]. (who.int)
  • The possible and morbidity [ 1 ]. (who.int)
  • In vivo, lung damage, inflammation and alteration in cytokines were observed 1 day after inhalation exposure, and this response returned to air control exposure levels by day 7. (cdc.gov)
  • These CSC-like cells, which express a high level of Cav-1, displayed increased cell migration and invasion, compared with their non-CSC counterpart. (cdc.gov)
  • Replication Study: Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis. (nih.gov)
  • Endostatin is derived from the noncollagenous domain 1 (NC1) at the C-terminus of collagen type XVIII. (who.int)
  • Conversely the typical distribution of caveolin-3 in myocyte sarcolemma was dramatically altered in S-MI rats, resulting in a heterogeneous pattern throughout the septum. (inserm.fr)
  • Structural analysis of the P132L disease mutation in caveolin-1 reveals its role in the assembly of oligomeric complexes. (bvsalud.org)
  • Role of Caveolin-1 in carbon nanotube -induced stem-like cells and tumorigenesis. (cdc.gov)
  • Our findings provide novel evidence for the induction of CSC-like cells by chronic SWCNT exposure, which are likely a driving force of SWCNT tumorigenesis, and demonstrated a positive regulatory role of Cav-1. (cdc.gov)
  • We have developed a DNA aptamer (D10) against Plasmodium falciparum 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), the second enzyme of this metabolic route. (ibecbarcelona.eu)
  • Esters were produced using cinnamic acid as the acylating agent and citronella essential oil (3:1) in heptane and 15 wt% NS 88011 enzyme as biocatalysts, at 70 °C and 150 rpm. (bvsalud.org)
  • In the insecticidal activity against Aedes aegypti larvae, was obtained LC50 of 111.84 μg mL-1 for the essential oil of citronella and 86.30 μg mL-1 for the esterified oils obtained with the enzyme NS 88011, indicating high toxicity of the esters. (bvsalud.org)
  • Non-existence of caveolin-1 gene mutations in human breast cancer. (cdc.gov)
  • Breast cancer research and treatment 2012 Jan 131 (1): 307-10. (cdc.gov)
  • Serum starved Swiss 3T3 cells were stimulated with the Cdc42 activating compound EGF and Cdc42 activation was measured with the G-LISA method (Fig 1 and 2). (cytoskeleton.com)
  • Swiss 3T3 cells were serum starved (SS) for 16 h at 1% serum and 8 h with 0% serum and treated with EGF (100 ng/ml for 2 min). (cytoskeleton.com)
  • A tendency to lower NHE-1 levels was seen, but highly increased ICAM1 expression in cells and human brains correlated with increased transcytosis and brain distribution in mice. (ibecbarcelona.eu)
  • Caveolin-1 expression and GM1 transcytosis were also reduced, yet increased GM1 levels seemed to compensate, providing similar NC brain distribution in NPD vs. control mice. (ibecbarcelona.eu)
  • In this study, we investigate whether (i) changes in caveolin abundance and/or distribution occur during cardiac aging and failure in rat, and (ii) the process could influence NO synthase (NOS) activity. (inserm.fr)
  • siRNA-mediated KID of CAV-1 in 3T3-L1 adipocytes also resulted in inhibition of CL-stimulated phosphorylation of HSL (hormone-sensitive lipase) and perilipin A, and of lipolysis. (lu.se)
  • For the toxicity to Artemia salina LC50 results of 5.29 μg mL-1 were obtained for the essential oil and 4.36 μg mL-1 for the esterified oils obtained with NS 88011. (bvsalud.org)
  • Similar results were obtained in adipocytes from Cav-1-deficient mice. (lu.se)
  • The goal of these policies is to establish collective guidelines to increase health and quality of life through prevention, treatment of disease and health behavior promotion 1 . (bvsalud.org)
  • Significant association of caveolin-1 single nucleotide polymorphisms with childhood leukemia in Taiwan. (cdc.gov)
  • Abstract This study evaluated the effect of the volatile oil of Alpinia zerumbet (VOAz) on caveolin-1 gene expression and muscular fibrosis. (bvsalud.org)
  • Collagen quality was assessed by histology and the expression of the caveolin-1 (CAV-1) gene was evaluated using qPCR. (bvsalud.org)
  • siRNA (small interfering RNA)-mediated KD (knockdown) of CAV-1 (caveolin-1) in 3T3-L1 adipocytes resulted in down-regulation of expression of membrane-associated PDE3B. (lu.se)
  • This study was conducted between Janu- ing region, Flk-1/KDR (VEGFR-2) [ 2 ]. (who.int)
  • Overexpression of Cav-1 further promoted the CSC induction, cell motility, and in vivo tumorigenesis. (cdc.gov)
  • In the context of auto-immunity, with sustained immune activation, the injury and repair phases persist and lead to scar tissue formation that disrupts organ architecture and function with a frequently fatal outcome (Figure 1 ). (frontiersin.org)
  • The following search was performed: #1 AND #2. (bvsalud.org)
  • It interacts with 2 tyrosine kinases: fms-like tyrosine kinase-1, Flt-1 (VEGFR-1), and kinase domain-containing region, Flk-1/KDR (VEGFR-2) [2]. (who.int)
  • It was well documented that development of essential hypertension in humans is often associated with the elevated sympathetic nerve activity [ 1 , 2 , 3 ]. (biomedcentral.com)
  • There also was a dose-dependent increase in NF-kB/AP-1 activity. (cdc.gov)
  • The endothelium plays a vital proatherogenic and anti-atherogenic factors" 1 . (who.int)