Caveolin 2 is a binding partner of CAVEOLIN 1. It undergoes tyrosine phosphorylation by C-SRC PROTEIN PP60 and plays a regulatory role in CAVEOLAE formation.
Endocytic/exocytic CELL MEMBRANE STRUCTURES rich in glycosphingolipids, cholesterol, and lipid-anchored membrane proteins that function in ENDOCYTOSIS (potocytosis), transcytosis, and SIGNAL TRANSDUCTION. Caveolae assume various shapes from open pits to closed vesicles. Caveolar coats are composed of CAVEOLINS.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Detergent-insoluble CELL MEMBRANE components. They are enriched in SPHINGOLIPIDS and CHOLESTEROL and clustered with glycosyl-phosphatidylinositol (GPI)-anchored proteins.
A complex of polyene antibiotics obtained from Streptomyces filipinensis. Filipin III alters membrane function by interfering with membrane sterols, inhibits mitochondrial respiration, and is proposed as an antifungal agent. Filipins I, II, and IV are less important.
Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.
A partitioning within cells due to the selectively permeable membranes which enclose each of the separate parts, e.g., mitochondria, lysosomes, etc.
The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.
Cyclic GLUCANS consisting of seven (7) glucopyranose units linked by 1,4-glycosidic bonds.
The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. Each molecule of clathrin consists of three light chains (CLATHRIN LIGHT CHAINS) and three heavy chains (CLATHRIN HEAVY CHAINS) that form a structure called a triskelion. Clathrin also interacts with cytoskeletal proteins.
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Nonionic surfactant mixtures varying in the number of repeating ethoxy (oxy-1,2-ethanediyl) groups. They are used as detergents, emulsifiers, wetting agents, defoaming agents, etc. Octoxynol-9, the compound with 9 repeating ethoxy groups, is a spermatocide.
A homologous group of cyclic GLUCANS consisting of alpha-1,4 bound glucose units obtained by the action of cyclodextrin glucanotransferase on starch or similar substrates. The enzyme is produced by certain species of Bacillus. Cyclodextrins form inclusion complexes with a wide variety of substances.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Established cell cultures that have the potential to propagate indefinitely.
A CALCIUM-dependent, constitutively-expressed form of nitric oxide synthase found primarily in ENDOTHELIAL CELLS.
Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays.
Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.
Neoplasms composed of fatty tissue or connective tissue made up of fat cells in a meshwork of areolar tissue. The concept does not refer to neoplasms located in adipose tissue.
A subtype of dynamin found ubiquitously expressed in a variety of tissues.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Cells in the body that store FATS, usually in the form of TRIGLYCERIDES. WHITE ADIPOCYTES are the predominant type and found mostly in the abdominal cavity and subcutaneous tissue. BROWN ADIPOCYTES are thermogenic cells that can be found in newborns of some species and hibernating mammals.
Compounds containing carbohydrate or glycosyl groups linked to phosphatidylinositols. They anchor GPI-LINKED PROTEINS or polysaccharides to cell membranes.
Membrane-limited structures derived from the plasma membrane or various intracellular membranes which function in storage, transport or metabolism.
Short tracts of DNA sequence that are used as landmarks in GENOME mapping. In most instances, 200 to 500 base pairs of sequence define a Sequence Tagged Site (STS) that is operationally unique in the human genome (i.e., can be specifically detected by the polymerase chain reaction in the presence of all other genomic sequences). The overwhelming advantage of STSs over mapping landmarks defined in other ways is that the means of testing for the presence of a particular STS can be completely described as information in a database.
A family of high molecular weight GTP phosphohydrolases that play a direct role in vesicle transport. They associate with microtubule bundles (MICROTUBULES) and are believed to produce mechanical force via a process linked to GTP hydrolysis. This enzyme was formerly listed as EC
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
An NADPH-dependent enzyme that catalyzes the conversion of L-ARGININE and OXYGEN to produce CITRULLINE and NITRIC OXIDE.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The extension of endometrial tissue (ENDOMETRIUM) into the MYOMETRIUM. It usually occurs in women in their reproductive years and may result in a diffusely enlarged uterus with ectopic and benign endometrial glands and stroma.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Cytoplasmic vesicles formed when COATED VESICLES shed their CLATHRIN coat. Endosomes internalize macromolecules bound by receptors on the cell surface.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Centrifugation using a rotating chamber of large capacity in which to separate cell organelles by density-gradient centrifugation. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The protein constituents of muscle, the major ones being ACTINS and MYOSINS. More than a dozen accessory proteins exist including TROPONIN; TROPOMYOSIN; and DYSTROPHIN.
Amino acids containing an aromatic side chain.
A glucose transport protein found in mature MUSCLE CELLS and ADIPOCYTES. It promotes transport of glucose from the BLOOD into target TISSUES. The inactive form of the protein is localized in CYTOPLASMIC VESICLES. In response to INSULIN, it is translocated to the PLASMA MEMBRANE where it facilitates glucose uptake.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Src-family kinases that associate with T-CELL ANTIGEN RECEPTOR and phosphorylate a wide variety of intracellular signaling molecules.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.
Unsaturated derivatives of the steroid androstane containing at least one double bond at any site in any of the rings.
Transport proteins that carry specific substances in the blood or across cell membranes.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
A PROTEIN-TYROSINE KINASE family that was originally identified by homology to the Rous sarcoma virus ONCOGENE PROTEIN PP60(V-SRC). They interact with a variety of cell-surface receptors and participate in intracellular signal transduction pathways. Oncogenic forms of src-family kinases can occur through altered regulation or expression of the endogenous protein and by virally encoded src (v-src) genes.
A genus of owlet moths of the family Noctuidae. These insects are used in molecular biology studies during all stages of their life cycle.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
An enzyme that catalyzes the oxidation of cholesterol in the presence of molecular oxygen to 4-cholesten-3-one and hydrogen peroxide. The enzyme is not specific for cholesterol, but will also oxidize other 3-hydroxysteroids. EC
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A subtype of thioredoxin reductase found primarily in the CYTOSOL.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
A heterogeneous group of inherited MYOPATHIES, characterized by wasting and weakness of the SKELETAL MUSCLE. They are categorized by the sites of MUSCLE WEAKNESS; AGE OF ONSET; and INHERITANCE PATTERNS.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Proteins prepared by recombinant DNA technology.
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.
A 700-kDa cytosolic protein complex consisting of seven equimolar subunits (alpha, beta, beta', gamma, delta, epsilon and zeta). COATOMER PROTEIN and ADP-RIBOSYLATION FACTOR 1 are principle components of COAT PROTEIN COMPLEX I and are involved in vesicle transport between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.
GPI-linked membrane proteins broadly distributed among hematopoietic and non-hematopoietic cells. CD55 prevents the assembly of C3 CONVERTASE or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex.
A low affinity receptor that binds NERVE GROWTH FACTOR; BRAIN-DERIVED NEUROTROPHIC FACTOR; NEUROTROPHIN 3; and neurotrophin 4.
A large group of membrane transport proteins that shuttle MONOSACCHARIDES across CELL MEMBRANES.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles is covered with a lattice-like network of the protein CLATHRIN. Shortly after formation, however, the clathrin coat is removed and the vesicles are referred to as ENDOSOMES.
Enzymes that hydrolyze GTP to GDP. EC 3.6.1.-.
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
A specific monosialoganglioside that accumulates abnormally within the nervous system due to a deficiency of GM1-b-galactosidase, resulting in GM1 gangliosidosis.
Lipids containing at least one monosaccharide residue and either a sphingoid or a ceramide (CERAMIDES). They are subdivided into NEUTRAL GLYCOSPHINGOLIPIDS comprising monoglycosyl- and oligoglycosylsphingoids and monoglycosyl- and oligoglycosylceramides; and ACIDIC GLYCOSPHINGOLIPIDS which comprises sialosylglycosylsphingolipids (GANGLIOSIDES); SULFOGLYCOSPHINGOLIPIDS (formerly known as sulfatides), glycuronoglycosphingolipids, and phospho- and phosphonoglycosphingolipids. (From IUPAC's webpage)
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
GTP-BINDING PROTEINS that contain three non-identical subunits. They are found associated with members of the seven transmembrane domain superfamily of G-PROTEIN-COUPLED RECEPTORS. Upon activation the GTP-BINDING PROTEIN ALPHA SUBUNIT of the complex dissociates leaving a dimer of a GTP-BINDING PROTEIN BETA SUBUNIT bound to a GTP-BINDING PROTEIN GAMMA SUBUNIT.
A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.
An essential amino acid that is required for the production of HISTAMINE.
A family of heterotrimeric GTP-binding protein alpha subunits that were originally identified by their ability to inhibit ADENYLYL CYCLASES. Members of this family can couple to beta and gamma G-protein subunits that activate POTASSIUM CHANNELS. The Gi-Go part of the name is also spelled Gi/Go.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
The entering of cells by viruses following VIRUS ATTACHMENT. This is achieved by ENDOCYTOSIS, by direct MEMBRANE FUSION of the viral membrane with the CELL MEMBRANE, or by translocation of the whole virus across the cell membrane.

The dually acylated NH2-terminal domain of gi1alpha is sufficient to target a green fluorescent protein reporter to caveolin-enriched plasma membrane domains. Palmitoylation of caveolin-1 is required for the recognition of dually acylated g-protein alpha subunits in vivo. (1/1802)

Here we investigate the molecular mechanisms that govern the targeting of G-protein alpha subunits to the plasma membrane. For this purpose, we used Gi1alpha as a model dually acylated G-protein. We fused full-length Gi1alpha or its extreme NH2-terminal domain (residues 1-32 or 1-122) to green fluorescent protein (GFP) and analyzed the subcellular localization of these fusion proteins. We show that the first 32 amino acids of Gi1alpha are sufficient to target GFP to caveolin-enriched domains of the plasma membrane in vivo, as demonstrated by co-fractionation and co-immunoprecipitation with caveolin-1. Interestingly, when dual acylation of this 32-amino acid domain was blocked by specific point mutations (G2A or C3S), the resulting GFP fusion proteins were localized to the cytoplasm and excluded from caveolin-rich regions. The myristoylated but nonpalmitoylated (C3S) chimera only partially partitioned into caveolin-containing fractions. However, both nonacylated GFP fusions (G2A and C3S) no longer co-immunoprecipitated with caveolin-1. Taken together, these results indicate that lipid modification of the NH2-terminal of Gi1alpha is essential for targeting to its correct destination and interaction with caveolin-1. Also, a caveolin-1 mutant lacking all three palmitoylation sites (C133S, C143S, and C156S) was unable to co-immunoprecipitate these dually acylated GFP-G-protein fusions. Thus, dual acylation of the NH2-terminal domain of Gi1alpha and palmitoylation of caveolin-1 are both required to stabilize and perhaps regulate this reciprocal interaction at the plasma membrane in vivo. Our results provide the first demonstration of a functional role for caveolin-1 palmitoylation in its interaction with signaling molecules.  (+info)

The Npc1 mutation causes an altered expression of caveolin-1, annexin II and protein kinases and phosphorylation of caveolin-1 and annexin II in murine livers. (2/1802)

We have previously demonstrated (1) an increased expression of caveolin-1 in murine heterozygous and homozygous Niemann-Pick type C (NPC) livers, and (2) an increased concentration of unesterified cholesterol in a detergent insoluble caveolae-enriched fraction from homozygous livers. To define further the relationship between caveolin-1 function and the cholesterol trafficking defect in NPC, we examined the expression and distribution of additional caveolar and signal transduction proteins. The expression of annexin II was significantly increased in homozygous liver homogenates and the Triton X-100 insoluble floating fraction (TIFF). Phosphoamino acid analysis of caveolin-1 and annexin II from the homozygous TIFF demonstrated an increase in serine and tyrosine phosphorylation, respectively. To determine the basis for increased phosphorylation of these proteins, the expression and distribution of several protein kinases was examined. The expression of PKCalpha, PKCzeta and pp60-src (protein kinases) were significantly increased in both heterozygous and homozygous liver homogenates, while PKCdelta was increased only in homozygous livers. Of the protein kinases analyzed, only CK IIalpha was significantly enriched in the heterozygous TIFF. Finally, the concentration of diacylglycerol in the homozygous TIFF was significantly increased and this elevation may modulate PKC distribution and function. These results provide additional evidence for involvement of a caveolin-1 containing cellular fraction in the pathophysiology of NPC and also suggest that the Npc1 gene product may directly or indirectly, regulate the expression and distribution of signaling molecules.  (+info)

Hypercholesterolemia decreases nitric oxide production by promoting the interaction of caveolin and endothelial nitric oxide synthase. (3/1802)

Hypercholesterolemia is a central pathogenic factor of endothelial dysfunction caused in part by an impairment of endothelial nitric oxide (NO) production through mechanisms that remain poorly characterized. The activity of the endothelial isoform of NO synthase (eNOS) was recently shown to be modulated by its reciprocal interactions with the stimulatory Ca2+-calmodulin complex and the inhibitory protein caveolin. We examined whether hypercholesterolemia may reduce NO production through alteration of this regulatory equilibrium. Bovine aortic endothelial cells were cultured in the presence of serum obtained from normocholesterolemic (NC) or hypercholesterolemic (HC) human volunteers. Exposure of endothelial cells to the HC serum upregulated caveolin abundance without any measurable effect on eNOS protein levels. This effect of HC serum was associated with an impairment of basal NO release paralleled by an increase in inhibitory caveolin-eNOS complex formation. Similar treatment with HC serum significantly attenuated the NO production stimulated by the calcium ionophore A23187. Accordingly, higher calmodulin levels were required to disrupt the enhanced caveolin-eNOS heterocomplex from HC serum-treated cells. Finally, cell exposure to the low-density lipoprotein (LDL) fraction alone dose-dependently reproduced the inhibition of basal and stimulated NO release, as well as the upregulation of caveolin expression and its heterocomplex formation with eNOS, which were unaffected by cotreatment with antioxidants. Together, our data establish a new mechanism for the cholesterol-induced impairment of NO production through the modulation of caveolin abundance in endothelial cells, a mechanism that may participate in the pathogenesis of endothelial dysfunction and the proatherogenic effects of hypercholesterolemia.  (+info)

Regulation of G protein-coupled receptor kinases by caveolin. (4/1802)

G protein-coupled receptor kinases (GRKs) have been principally characterized by their ability to phosphorylate and desensitize G protein-coupled receptors. However, recent studies suggest that GRKs may have more diverse protein/protein interactions in cells. Based on the identification of a consensus caveolin binding motif within the pleckstrin homology domain of GRK2, we tested the direct binding of purified full-length GRK2 to various glutathione S-transferase-caveolin-1 fusion proteins, and we discovered a specific interaction of GRK2 with the caveolin scaffolding domain. Interestingly, analysis of GRK1 and GRK5, which lack a pleckstrin homology domain, revealed in vitro binding properties similar to those of GRK2. Maltose-binding protein caveolin and glutathione S-transferase-GRK fusion proteins were used to map overlapping regions in the N termini of both GRK2 and GRK5 that appear to mediate conserved GRK/caveolin interactions. In vivo association of GRK2 and caveolin was suggested by co-fractionation of GRK2 with caveolin in A431 and NIH-3T3 cells and was further supported by co-immunoprecipitation of GRK2 and caveolin in COS-1 cells. Functional significance for the GRK/caveolin interaction was demonstrated by the potent inhibition of GRK-mediated phosphorylation of both receptor and peptide substrates by caveolin-1 and -3 scaffolding domain peptides. These data reveal a novel mode for the regulation of GRKs that is likely to play an important role in their cellular function.  (+info)

Analysis of the CAVEOLIN-1 gene at human chromosome 7q31.1 in primary tumours and tumour-derived cell lines. (5/1802)

We identified CAVEOLIN-1 as a candidate for a tumour suppressor gene mapping to human chromosome 7q31.1. A number of studies suggest that caveolin could function as a tumour suppressor. Expression of caveolin, and in turn the number of caveolae within a cell, are inversely correlated with the transforming ability of numerous oncoproteins, including H-ras, v-abl, and bcr-abl, and caveolin is a major transformation-dependent substrate of v-src. Heterologous expression of caveolin has been shown to abrogate anchorage-independent growth and induce apoptosis in transformed fibroblasts and also to suppress anchorage-independent growth in human mammary carcinoma cells. We have analysed the status and expression of the human CAVEOLIN-1 gene in primary tumours and tumour-derived cell lines. We found no evidence for mutation of CAVEOLIN-1 in human cancers. Additionally, we found that while the first two exons of CAVEOLIN-1 are associated with a CpG island, this is not methylated in either primary tumours or in tumour-derived cell lines in which Caveolin-1 expression is low or undetectable. The level of expression of Caveolin-1 does not correlate with loss of heterozygosity at the CAVEOLIN-1 locus in these same cell lines. Contrary to other published studies, we have shown that CAVEOLIN-1 is not expressed in normal breast ductal epithelial cells in vivo. CAVEOLIN-1 is however highly expressed in breast myoepithelial cells and its expression is retained in tumours derived from breast myoepithelium. Together our data refute a role for CAVEOLIN-1 as a breast tumour suppressor gene in vivo.  (+info)

A role for caveolin and the urokinase receptor in integrin-mediated adhesion and signaling. (6/1802)

The assembly of signaling molecules surrounding the integrin family of adhesion receptors remains poorly understood. Recently, the membrane protein caveolin was found in complexes with beta1 integrins. Caveolin binds cholesterol and several signaling molecules potentially linked to integrin function, e.g., Src family kinases, although caveolin has not been directly implicated in integrin-dependent adhesion. Here we report that depletion of caveolin by antisense methodology in kidney 293 cells disrupts the association of Src kinases with beta1 integrins resulting in loss of focal adhesion sites, ligand-induced focal adhesion kinase (FAK) phosphorylation, and adhesion. The nonintegrin urokinase receptor (uPAR) associates with and stabilizes beta1 integrin/caveolin complexes. Depletion of caveolin in uPAR-expressing 293 cells also disrupts uPAR/integrin complexes and uPAR-dependent adhesion. Further, beta1 integrin/caveolin complexes could be disassociated by uPAR-binding peptides in both uPAR-transfected 293 cells and human vascular smooth muscle cells. Disruption of complexes by peptides in intact smooth muscle cells blocks the association of Src family kinases with beta1 integrins and markedly impairs their migration on fibronectin. We conclude that ligand-induced signaling necessary for normal beta1 integrin function requires caveolin and is regulated by uPAR. Caveolin and uPAR may operate within adhesion sites to organize kinase-rich lipid domains in proximity to integrins, promoting efficient signal transduction.  (+info)

Visualization of caveolin-1, a caveolar marker protein, in living cells using green fluorescent protein (GFP) chimeras. The subcellular distribution of caveolin-1 is modulated by cell-cell contact. (7/1802)

Caveolin-1, a suspected tumor suppressor, is a principal protein component of caveolae in vivo. Recently, we have shown that NIH 3T3 cells harboring anti-sense caveolin-1 exhibit a loss of contact inhibition and anchorage-independent growth. These observations may be related to the ability of caveolin-1 expression to positively regulate contact inhibition. In order to understand the postulated role of caveolin-1 in contact inhibition, it will be necessary to follow the distribution of caveolins in living cells in response to a variety of stimuli, such as cell density. Here, we visualize the distribution of caveolin-1 in living normal NIH 3T3 cells by creating GFP-fusion proteins. In many respects, the behavior of these GFP-caveolin-1 fusion proteins is indistinguishable from endogenous caveolin-1. These GFP-caveolin-1 fusion proteins co-fractionated with endogenous caveolin-1 using an established protocol that separates caveolae-derived membranes from the bulk of cellular membranes and cytosolic proteins, and co-localized with endogenous caveolin-2 in vivo as seen by immunofluorescence microscopy. We show here that as NIH 3T3 cells become confluent, the distribution of GFP-caveolin-1 and endogenous caveolin-1 shifts to areas of cell-cell contact, coincident with contact inhibition. However, unlike endogenous caveolin-1, the levels of GFP-caveolin-1 expression are unaffected by changes in cell density, serum starvation, or growth factor stimulation. These results are consistent with the idea that the levels of endogenous caveolin-1 are modulated by either transcriptional or translational control, and that this modulation is separable from density-dependent regulation of the distribution of caveolin-1. These studies provide a new living-model system for elucidating the dynamic mechanisms underlying the density-dependent regulation of the distribution of caveolin-1 and how this relates to contact inhibition.  (+info)

Tyrosine-phosphorylated caveolin-1: immunolocalization and molecular characterization. (8/1802)

Caveolin-1 was discovered as a major substrate for v-Src, but the effect of its tyrosine phosphorylation has not been known. We generated a specific antibody (PY14) to caveolin-1 phosphorylated at tyrosine 14 and studied the significance of the modification. By Western blotting of lysates of v-Src-expressing cells, PY14 recognized not only a 22-kDa band (the position of nonphosphorylated caveolin-1) but bands at 23-24 and 25 kDa. Bands of slower mobility were diminished by dephosphorylation and were also observed for mutant caveolin-1 lacking tyrosine 14. By immunofluorescence microscopy, PY14 did not label normal cells but detected large dots in v-Src-expressing cells. Immunoelectron microscopy revealed that the dots corresponded to aggregated caveolae and/or vesicles of various sizes; besides, the label was observed in intramembrane particle-free areas in the plasma membrane, which appeared to have been formed by fusion of flattened caveolae. A positive reaction with PY14 was found in normal cells after vanadate or pervanadate treatment; it occurred mainly at 22 kDa by Western blotting and was not seen as large dots by immunofluorescence microscopy. Detergent solubility, oligomerization, and association with caveolin-2 were observed similarly for caveolin-1 in normal and v-Src-expressing cells. The results indicate that phosphorylation of caveolin-1 in v-Src-expressing cells occurs at multiple residues and induces flattening, aggregation, and fusion of caveolae and/or caveolae-derived vesicles.  (+info)

Phospholipase D2 (PLD2) has been found localized in low-density caveolin-rich membrane microdomains. Our previous study suggested that PLD2 and aquaporin 3 (AQP3) interact in these domains to inhibit keratinocyte proliferation and promote differentiation by cooperating to produce phosphatidylglycerol. To examine the effect of membrane microdomain localization on the PLD2/AQP3 signaling module and keratinocyte proliferation and differentiation, we treated mouse keratinocytes with 3 µM cell-permeable caveolin-1 scaffolding domain peptide or a negative control peptide and stimulated cell differentiation using a moderately elevated extracellular calcium concentration (125 uM) to maximally promote differentiation and phosphatidylglycerol production. Cell proliferation, differentiation, total PLD activity, phosphatidylglycerol levels, and AQP3 activity were monitored. The caveolin-1 scaffolding domain peptide itself had no effect on phosphatidylglycerol levels or keratinocyte proliferation or ...
Thank you for sending your work entitled Registered report: Biomechanical remodeling of the microenvironment by stromal Cav1 favors tumor invasion and metastasis for consideration at eLife. Your article has been evaluated by Fiona Watt (Senior editor), a Reviewing editor, and four reviewers, one of whom has direct statistical expertise.. The following individuals responsible for the peer review of your submission have agreed to reveal their identity: Miguel Del Pozo (Reviewer 1), Peter Friedl (Reviewer 2), and Dawn Teare (Reviewer 4). Reviewer 3 remains anonymous.. The Reviewing editor and the reviewers discussed their comments before we reached this decision, and the Reviewing editor has assembled the following comments to help you prepare a revised submission.. Overall, the reviewers agree on the choice of the key experiments and the experimental approach. However, a number of points have been raised that need to be addressed in a revised Registered Report before proceeding with the ...
Abcam provides specific protocols for Anti-Caveolin-3 antibody (ab87770) : Western blot protocols, Immunohistochemistry protocols
TY - JOUR. T1 - Caveolin-2 is targeted to lipid droplets, a new membrane domain in the cell. AU - Fujimoto, Toyoshi. AU - Kogo, Hiroshi. AU - Ishiguro, Kimiko. AU - Tauchi, Kumi. AU - Nomura, Ryuji. PY - 2001/3/5. Y1 - 2001/3/5. N2 - Caveolin-1 and -2 constitute a framework of caveolae in nonmuscle cells. In the present study, we showed that caveolin-2, especially its β isoform, is targeted to the surface of lipid droplets (LD) by immunofluorescence and immunoelectron microscopy, and by subcellular fractionation. Brefeldin A treatment induced further accumulation of caveolin-2 along with caveolin-1 in LD. Analysis of mouse caveolin-2 deletion mutants revealed that the central hydrophobic domain (residues 87-119) and the NH2-terminal (residues 70-86) and COOH-terminal (residues 120-150) hydrophilic domains are all necessary for the localization in LD. The NH2- and COOH-terminal domains appeared to be related to membrane binding and exit from ER, respectively, implying that caveolin-2 is ...
Caveolin-2兔单克隆抗体[EPR2220(3)](ab124883)可与人样本反应并经WB, Flow Cyt实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Mouse monoclonal Caveolin-1 antibody [AT4C1]. Validated in WB, ICC/IF and tested in Mouse, Human. Immunogen corresponding to recombinant fragment.
Rabbit Monoclonal Anti-Caveolin-1 Antibody (SP43). Caveolae Marker, Endosome Marker. Validated: IHC, IHC-P. Tested Reactivity: Human. 100% Guaranteed.
Mouse Monoclonal Anti-Caveolin-1 Antibody (7C8) [DyLight 488]. Caveolae Marker, Endosome Marker. Validated: WB, Flow, ICC/IF, IHC-Fr, IHC-P. Tested Reactivity: Human, Mouse, Rat, and more. 100% Guaranteed.
Plasmid CAV1-mEGFP from Dr. Ari Heleniuss lab contains the insert caveolin-1 and is published in Traffic. 2010 Mar . 11(3):361-82. This plasmid is available through Addgene.
Caveolae are plasma membrane microdomains that localise receptors and signalling intermediates within an environment where they can efficiently trigger downstream events. While present in most cells types, they are particularly abundant in vascular smooth muscle cells and endothelial cells (ECs), where they regulate low density lipoprotein trans-cytosis, NO production and inflammation. Cavin-1 is a protein required for caveolae maturation, such that its deletion results in no detectable caveolae and the down-regulation of caveolin scaffolding proteins. However, the cellular processes regulating cavin-1 function are poorly understood. Proteomic screening and biochemical experiments have identified cavin-1 as a novel interacting protein of the cytokine-inducible E3 ubiquitin ligase component suppressor of cytokine signalling-3 (SOCS3), a key inhibitor of IL-6-mediated pro-inflammatory signalling in ECs. We hypothesise d that the SOCS3/cavin-1 interaction may be important in controlling caveola ...
Several exogenous and endogenous cargo proteins are internalized independently of clathrin, including the bacterial Shiga toxin. The mechanisms underlying early steps of clathrin-independent uptake remain largely unknown. In this study, we have designed a protocol to obtain gradient fractions containing Shiga toxin internalization intermediates. Using stable isotope labeling with amino acids in cell culture (SILAC) and quantitative mass spectrometry, Rab12 was found in association with these very early uptake carriers. The localization of the GTPase on Shiga toxin-induced plasma membrane invaginations was shown by fluorescence microscopy in cells transfected with GFP-Rab12. Furthermore, using a quantitative biochemical assay, it was found that the amount of receptor-binding B-subunit of Shiga toxin reaching the trans-Golgi/TGN membranes was decreased in Rab12-depleted cells, and that cells were partially protected against intoxication by Shiga-like toxin 1 under these conditions. These findings ...
The complex interplay of the tumour microenvironment (TME) and its role in disease progression and response to therapy is poorly understood. The majority of studies to date focus on individual components or molecules within the TME and so lack the power correlative analysis. Here we have performed a multi-parameter analysis of the TME in 62 resectable non-small cell lung cancer (NSCLC) specimens detailing number and location of immune infiltrate, assessing markers of cancer-associated fibroblasts, caveolin-1 and tenascin-C, and correlating with clinicopathological details, as well as markers of disease progression such as epithelial-to-mesenchymal transition (EMT). The influence of individual parameters on overall survival was determined in univariate and multivariate analysis and the combination of risk factors and interplay between components analysed. Low numbers of CD8 T cells, low stromal levels of caveolin-1 or high levels of tenascin-C were significant prognostic markers of decreased ...
TY - JOUR. T1 - Circulating cardiovascular disease risk factors and signaling in endothelial cell caveolae. AU - Mineo, Chieko. AU - Shaul, Philip W.. PY - 2006/4/1. Y1 - 2006/4/1. N2 - Caveolae are a subset of lipid rafts that are prevalent on the plasma membrane of endothelial cells. They compartmentalize signal transduction molecules which regulate multiple endothelial functions including the production of nitric oxide (NO) by the caveolae resident enzyme endothelial NO synthase (eNOS). Recent studies have demonstrated that circulating factors known to modify cardiovascular disease risk regulate signaling in endothelial cell caveolae. In particular, high density lipoprotein (HDL) maintains the lipid environment in caveolae, thereby promoting the retention and function of eNOS in the domain, and it also causes direct activation of eNOS via scavenger receptor type BI (SR-BI)-induced kinase signaling. Estrogen binding to estrogen receptors (ER) in caveolae also activates eNOS, and this occurs ...
TY - JOUR. T1 - AMP-dependent kinase inhibits oxidative stress-induced caveolin-1 phosphorylation and endocytosis by suppressing the dissociation between c-Abl and Prdx1 proteins in endothelial cells. AU - Takeuchi, Kimio. AU - Morizane, Yuki. AU - Kamami-Levy, Cynthia. AU - Suzuki, Jun. AU - Kayama, Maki. AU - Cai, Wenyi. AU - Miller, Joan W.. AU - Vavvas, Demetrios G.. PY - 2013/7/12. Y1 - 2013/7/12. N2 - Caveolin-1 is the primary structural component of endothelial caveolae that is essential for transcellular trafficking of albumin and is also a critical scaffolding protein that regulates the activity of signaling molecules in caveolae. Phosphorylation of caveolin-1 plays a fundamental role in the mechanism of oxidant-induced vascular hyper permeability. However, the regulatory mechanism of caveolin-1 phosphorylation remains unclear. Here we identify a previously unexpected role for AMPKin inhibition of caveolin-1 phosphorylation under oxidative stress. A pharmacological activator of AMPK, ...
Plays an important role in caveolar biogenesis and morphology. Regulates caveolae morphology by inducing membrane curvature within caveolae (PubMed:19525939). Plays a role in caveola formation in a tissue-specific manner. Required for the formation of caveolae in the lung and fat endothelia but not in the heart endothelia. Negatively regulates the size or stability of CAVIN complexes in the lung endothelial cells. May play a role in targeting PRKCA to caveolae (By similarity).
Caveolin-1 (Cav-1) is a 21?kDa protein enriched in caveolae and continues to be implicated in oncogenic cell transformation CHC tumorigenesis and metastasis. response to chemotherapy and radiation and tumor growth. We found Cav-1 is definitely overexpressed in human being Personal computer cell lines mouse models and human being pancreatic tumors and is associated with worse tumor grade and clinical results. In Personal computer cell lines disruption/depletion of caveolae/Cav-1 reduces proliferation colony formation and invasion. Radiation and chemotherapy up-regulate Cav-1 manifestation while Cav-1 depletion induces both chemosensitization and radiosensitization through modified apoptotic and DNA restoration signaling. and and transgene (KPC mouse) (Fig. 1B). Cav-1 manifestation was also tested on a cells microarray of 110 individuals with pancreatic malignancy and obtained semi-quantitatively for low versus high CHC manifestation. While Cav-1 is definitely virtually absent in pancreatic ...
Caveolin-1 (Cav-1), a major structural component of cell membrane caveolae, is involved in a variety of intracellular signaling pathways as well as transmembrane transport. Cav-1, as a scaffolding protein, modulates signal transduction associated with cell cycle progression, cellular senescence, cell proliferation and death, lipid homeostasis, etc. Cav-1 is also thought to regulate the expression or activity of oncoproteins, such as Src family kinases, H-Ras, protein kinase C, epidermal growth factor, extracellular signal-regulated kinase, and endothelial nitric oxide synthase. Because of its frequent overexpression or mutation in various tumor tissues and cancer cell lines, Cav-1 has been speculated to play a role as an oncoprotein in cancer development and progression. In contrast, Cav-1 may also function as a tumor suppressor, depending on the type of cancer cells and/or surrounding -stromal cells in the tumor microenvironment as well as the stage of tumors ...
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity).
Proteins and other substances can cross the endothelial layer that lines a blood vessel via two routes. Caveolin-1 is essential for both, Siddiqui et al. show.. Researchers already knew that caveolin-1 was necessary for transcellular protein trafficking, in which macromolecules such as albumin enter an endothelial cell from the bloodstream and exit on the tissue side. Caveolae swallow these molecular travelers and bundle them into vesicles that wend through the cell. In an alternative pathway, known as the paracellular route, molecules slip between the cells of the endothelial layer, passing through the adherens junctions that fasten adjacent cells together. Previous work showed that adherens junctions become permeable in mice lacking caveolin-1, suggesting that the protein helps seal the junctions.. Siddiqui et al. dissected the molecular chain of events that connects caveolin-1 to adherens junction integrity. Loss of caveolin-1 activated the enzyme eNOS, which spawns nitric oxide (NO) that ...
Caveolae are specialised forms of lipid rafts in the plasma membrane of most cells. They form dynamic assemblies of sphingolipids and cholesterol containing scaffolding domains with different affinities for proteins resulting in a variety of functions [1]. The constitutive Cav-1 protein is distributed ubiquitously, while Cav-2 is usually associated with Cav-1 [3, 28]. Although Cav-3 is thought to be muscle specific and is expressed in striated muscles [3, 28, 29], we and others have found that Cav-3 is not expressed within human ASM [6, 30]. Recent studies have established that ASM caveolae contain a number of proteins important to [Ca2+]i regulation (e.g. agonist receptors, Ca2+ influx channels, and force regulatory proteins such as RhoA). In canine ASM, it has been established that caveolar-enriched membrane fractions express Cav-1, L-type Ca2+ channels and plasma membrane Ca2+ ATPase, but not SR proteins such as IP3R or RyR channels [31].. In ASM of different species, in addition to Ca2+ ...
Caveolae are small, cholesterol-rich, hydrophobic membrane domains, characterized by the presence of the protein caveolin and involved in several cellular processes, including clathrin-independent endocytosis, the regulation and transport of cellular cholesterol, and signal transduction. Recently, c …
Lipids are the energy source used during liver regeneration. The research group, led by Dr. Albert Pol and with members from IDIBAPS, Universitat de Barcelona and Queensland University, unveils the essential role of the protein caveolin-1 in a fundamental process for liver cure after injury or transplant. Results also evidence the existence of cellular mechanisms by which excessive accumulation of lipids in the liver is a risk factor for the apparition of hepatic tumours.
Caveolin-3 (Cav-3) is a protein that has been implicated in t-tubule formation and function in cardiac ventricular myocytes. In cardiac hypertrophy and failure, Cav-3 expression decreases, t-tubule structure is disrupted and excitation-contraction coupling (ECC) is impaired. However, the extent to which the decrease in Cav-3 expression underlies these changes is unclear. We therefore investigated the structure and function of myocytes isolated from the hearts of Cav-3 KO mice. These mice showed cardiac dilatation and decreased ejection fraction in vivo, compared to WT controls. Isolated KO myocytes showed cellular hypertrophy and altered t-tubule structure, and decreased L-type Ca channel (LTCC) current (ICa) density. This decrease in density occurred predominantly in the t-tubules, with no change in total ICa, and was therefore a consequence of the increase in membrane area. Cav-3 KO had no effect on LTCC expression, and C3SD peptide, which mimics the scaffolding domain of Cav-3, had no effect ...
Caveolae, the sphingomy-cholesterol enriched microdomains, form a stable lipid matrix that act as an ordered support for receptor-mediated signaling events. It has been proposed that caveola composition changes in response to adverse extracellular conditions [35,36]. Consistent with this hypothesis, we found in the current study that HG treatment led to a decrease in the number of caveolae on the surface of LECs. We also found reduced levels of caveolin-1 mRNA and protein in HG-treated LECs, indicating that caveolin-1 expression was down-regulated at the transcription level. Although it has been proposed that caveolin-1 functions as a message center that compartmentalizes anti- and pro-apoptotic signaling molecules on the cell surface to regulate apoptosis [17], its role in regulating apoptosis remains controversial. In the current study, we found the levels of caveolin-1 reduced and apoptosis rate increased in HG-treated LECs, indicating that caveolin-1 may inhibit apoptosis. However, ...
where to get vector overexpressing caveolin-1? - posted in Molecular Cloning: Trying to overexpress caveolin-1 in human cancer cells...are there readymade products out there which already has cav-1 attached to a dependable promoter? If so, where should I look? Thanks in advance!
The scaffolding protein CAV1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to…
View mouse Cavin3 Chr7:105480083-105482300 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
The primary function of adipose tissue is to store energy in the form of triacylglycerol, which is hydrolyzed to fatty acids to supply other tissues with energy. While insulin promotes the storage of triacylglycerol, catecholamines stimulate its hydrolysis. The development of type II diabetes is strongly associated with obesity, indicating a role of triacylglycerol metabolism in the pathogenesis of diabetes. Caveolae are plasma membrane invaginations found in most cells but are highly abundant in adipocytes. Insulin receptors are localized in caveolae and their function depends on intact caveolae structures. In the present thesis work, mass spectrometry-based methodology allowed identification of a number of new proteins and their posttranslational modifications in caveolae of human adipocytes. Variable N-terminal acetylation and phosphorylation of caveolin-1α and caveolin-1β were identified, which might regulate the function of caveolae. The transcription regulator protein PTRF was identified ...
TY - CHAP. T1 - Role of caveolae in the airway. AU - Pabelick, Christina M. AU - Singh, Brij B.. AU - Prakash, Y.s.. PY - 2013/11/1. Y1 - 2013/11/1. N2 - Caveolae are flask-shaped invaginations of the plasma membrane that are rich in lipids and serve as microdomains to facilitate interactions between proteins at the membrane as well as intracellular components, thus modulating signal transduction, protein and lipid transport, and other processes. Constituent caveolar proteins such as caveolins and cavins also have scaffolding domains that anchor and regulate protein function. There is now evidence that caveolae and their constituent proteins are present in airway smooth muscle in a variety of species. Caveolae in airway cells contain or interact with molecules such as receptors, ion channels, and regulatory proteins that are key to the roles of airway epithelium and smooth muscle in regulating airway structure and function. Furthermore, caveolar protein expression and regulation appear to be ...
Recently, we reported that the activities of large conductance Ca2+-activated K+(BK) channels are inhibited by caveolae microdomain targeting in vascular endothelial cells. The molecular mechanism of this negative regulation of BK channels by caveolae is unknown. In this study, we tested the hypotheses that BK channels are inhibited by physical interaction with the scaffolding domain of caveolin-1, which alters the properties of the channel. Using HEK293 cells stably expressing hSlo (which encodes human BK channels) with and without transient transfection with caveolin-1, We found that hSlo channels were enriched in the low buoyant density fraction when co-expressed with caveolin-1 and the channels were co-immunoprecipitated by anti-caveolin-1 antibodies. Co-expression of caveolin-1 resulted in inhibition of whole-cell hSlo current densities from 77.0±18.0 pA/pF in control (HP=−60 mV, TP=+80 mV, 1 μM free Ca2+, n=10) to 12.0±3.9 pA/pF (n=8, p,0.01 vs. control). In contrast, co-expression of ...
Caveolae are specialized flask-shaped lipid rafts enriched in cholesterol, sphingolipids, and structural marker proteins termed caveolins. Caveolins are highly conserved hairpin loop-shaped, oligomeric proteins of 22-24 kDa. Besides the plasma cell membrane, caveolins are also present in mitochondri …
Caveolins act as scaffold proteins in multiprotein complexes and have been implicated in signaling by G protein-coupled receptors. Studies using knock-out mice suggest that beta(3)-adrenoceptor (beta(3)-AR) signaling is dependent on caveolin-1; however, it is not known whether caveolin-1 is associated with the beta(3)-AR or solely with downstream signaling proteins. We have addressed this question by examining the impact of membrane rafts and caveolin-1 on the differential signaling of mouse beta(3a)- and beta(3b)-AR isoforms that diverge at the distal C terminus. Only the beta(3b)-AR promotes pertussis toxin (PTX)-sensitive cAMP accumulation. When cells expressing the beta(3a)-AR were treated with filipin III to disrupt membrane rafts or transfected with caveolin-1 siRNA, the cyclic AMP response to the beta(3)-AR agonist CL316243 became PTX-sensitive, suggesting G alpha(i/o) coupling. The beta(3a)-AR C terminus, S (P-384) under bar PLNR (P-389) under bar DG (Y-392) under bar EGARP (P-398) under ...
Abstract: Low molecular weight protein tyrosine phosphatases (LMW-PTPs) are small enzymes that are ubiquitous in many organisms. They are important in biological processes such as cell proliferation, adhesion, migration, and invasiveness. LMW-PTP is expressed in mammalian cells as two isoforms (IF1 and IF2) originating through alternative splicing. We have previously shown that IF2 targets lipid rafts called caveolae and interacts with caveolin-1, their major structural protein. Caveolae are cholesterol- and sphingolipid-rich membrane microdomains that have been implicated in a variety of cellular functions, including signal transduction events. Caveolin-1 contains a scaffolding region that contributes to the binding of the protein to the plasma membrane and mediates protein omo- and etero-oligomerization. Interaction of many signaling molecules with the scaffolding domain sequesters them into caveolae and inhibits or suppresses their activities. Caveolin-interacting proteins usually have a ...
This is the first report that two different DIG fractions can be isolated from myelin by TX-100 extraction with sodium phosphate buffer lacking EDTA/EGTA, at room temperature. Both of these fractions have characteristics of GSL/cholesterol-enriched membrane domains based on their TX-100 insolubility, high ratios of cholesterol and GalC to phospholipid, enrichment in the GPI-linked protein NCAM120, and buoyancy on a sucrose density gradient. However, B2 also contained the cytoskeletal proteins actin and tubulin, and the raft markers flotillin-1, caveolin and GM1, and thus may consist of caveolae or other types of GSL/cholesterol-enriched membrane domain associated with cytoskeletal proteins. It does not contain non-membrane-associated cytoskeleton, which would sediment faster to a much higher density.. This finding of a caveolin-containing type of membrane domain associated with cytoskeletal proteins in myelin is also novel. Both caveolae and non-caveolar membrane domains have been found to ...
Caveolae. Caveolae were described in the fifties of the last century by P. Palade after observing animal tissues at transmission electron microscopy. They are small invaginations (45-80 nm) of the plasma membrane that can be observed in most of the eukaryotic cells. It was suggested that most of the caveolae become vesicles (but see below). Caveolae are abundant in endothelial cells, muscle cells and adipocytes. The membrane of the caveolae contains caveolin, as well as other integral proteins linked to glycosylphosphatidylinositol, many sphingolipids (sphingomyelin and glycosphingolipids), and is enriched in cholesterol. The presence of caveolin in a cell is enought to form caveolae. There are around 100 to 200 caveolin molecules in one caveola and there are different types of caveolin in one caveola. Caveolin 1 is expressed in smooth muscle cells and in most of the non muscle cells, and it is necessary for caveolae formation in these cells. Caveolin 2, which can be expressed in the same cells ...
This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3 end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy. [provided by RefSeq, Nov 2009 ...
Research in cultured cells present that activation of endothelial nitric oxide (Zero) synthase (eNOS) requires the dissociation of the enzyme from it is inhibitory association with caveolin-1 (Cav-1), as well as perhaps it is translocation from plasma membrane caveolae to other cellular compartments. microsomal small percentage. ACh-induced vasodilatation was connected with eNOS translocation towards the cytosolic and Golgi-enriched fractions. After 1.5, 3.0 or 6.0 min of application, 10 m ACh reduced the amount of membrane-bound eNOS by -13 4 %, -60 4 % and -19 17 INCB8761 (PF-4136309) %, respectively; at the same time factors, 100 m ACh decreased microsomal eNOS articles by -38 9 %, -61 16 % and -40 18 %, respectively (= 4-5). In every situations, microsomal Cav-1 articles did not transformation. The close ACh focus dependence as well as the concomitance INCB8761 (PF-4136309) between eNOS subcellular redistribution no release support the idea that eNOS translocation in the plasma membrane is ...
Caveolae are specialized domains present in the plasma membrane (PM) of most mammalian cell types. They function in signalling, membrane regulation, and endocytosis. We found that the Eps-15 homology domain-containing protein 2 (EHD2, an ATPase) associated with the static population of PM caveolae. Recruitment to the PM involved ATP binding, interaction with anionic lipids, and oligomerization into large complexes (60-75S) via interaction of the EH domains with intrinsic NPF/KPF motifs. Hydrolysis of ATP was essential for binding of EHD2 complexes to caveolae. EHD2 was found to undergo dynamic exchange at caveolae, a process that depended on a functional ATPase cycle. Depletion of EHD2 by siRNA or expression of a dominant-negative mutant dramatically increased the fraction of mobile caveolar vesicles coming from the PM. Overexpression of EHD2, in turn, caused confinement of cholera toxin B in caveolae. The confining role of EHD2 relied on its capacity to link caveolae to actin filaments. Thus, ...
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Bestselling lip plumper in new sleek packaging. Intensive lip treatment for smoother, fuller, younger-looking lips. Boosts hydration and collagen synthesis to banish wrinkles and build fullness and volume. Paraben-free.. Whats Inside: Unique Lip Response Technology is a synergistic trio of the most effective ingredients from the natural and scientific worlds. A botanical extract, from a species of the cork tree, is combined with two peptides that mimic the principal structural components of the lips. Sunflower Seed Oil is a potent emollient that helps to retain moisture and a healthy softness. Sodium Hyaluronate is a water-binding, plumping agent. Vitamins C & E lock in moisture and provide antioxidant protection.. How To Use: Apply directly onto center of lips, blending outwards. Apply over lipstick for a fuller-looking lip. ...
Directional cell migration is crucially dependent on the spatiotemporal control of intracellular signalling events. These events regulate polarized actin dynamics, resulting in protrusion at the front of the cell and contraction at the rear. The actin cytoskeleton is regulated through signalling by Rho-like GTPases, such as RhoA, which stimulates myosin-based contractility, and CDC42 and Rac1, which promote actin polymerization and protrusion. Here, we show that Rac1 binds the adapter protein caveolin-1 (Cav1) and that Rac1 activity promotes Cav1 accumulation at Rac1-positive peripheral adhesions. Using Cav1-deficient mouse fibroblasts and depletion of Cav1 expression in human epithelial and endothelial cells mediated by small interfering RNA and short hairpin RNA, we show that loss of Cav1 induces an increase in Rac1 protein and its activated, GTP-bound form. Cav1 controls Rac1 protein levels by regulating ubiquitylation and degradation of activated Rac1 in an adhesion-dependent fashion. ...
Polyclonal antibody for CAVEOLIN 1/CAV1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CAVEOLIN 1/CAV1 information: Molecular Weight: 20472 MW; Subcellular Localization: Golgi apparatus membrane; Peripheral
Received 6 November 2009. Accepted 1 December 2009. Uncorrected manuscript published online 3 December 2009. Published online 8 January 2010 ... Caveolins in Cancer Pathogenesis, Prevention and Therapy [4196990] - Caveolins play an important role in the pathogenesis of multiple cancers. This volume focuses mainly on the importance of Caveolin-1 in breast, prostate, lung, skin, colon, pancreatic and brain cancers. It also studies the role of Caveolin-3 in breast cancer.Caveolins are important structural proteins of Caveolae, small invaginations of the
Objectives Reduced caveolin-1 levels in lung fibroblasts from patients with scleroderma and the lungs of bleomycin-treated mice promote collagen overexpression and lung fibrosis. This study was undertaken to determine whether caveolin-1 is deficient in leucocytes from bleomycin-treated mice and patients with scleroderma and to examine the consequences of this deficiency and its reversal.. Methods Mice or cells received the caveolin-1 scaffolding domain (CSD) peptide to reverse the pathological effects of reduced caveolin-1 expression. In bleomycin-treated mice, the levels of caveolin-1 in leucocytes and the effect of CSD peptide on leucocyte accumulation in lung tissue were examined. To validate the results in human disease and to identify caveolin-1-regulated molecular mechanisms, monocytes and neutrophils were isolated from patients with scleroderma and control subjects and caveolin-1, extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), p38, CXC chemokine ...
Background: Muscle-restricted coiled-coil protein (MURC)/cavin-4 is a novel member of the cavin family that regulates caveolae function. Caveolae formation and function are also regulated by caveolin-3 in cardiomyocytes (CMs). Overexpression of caveolin-3 induces cardiac protection, and loss of caveolin-3 causes progressive cardiomyopathy. Mutations in MURC have been identified in patients with dilated cardiomyopathy. However, the role of MURC as a caveolar component protein remains unknown.. Methods and Results: To assess the role of MURC in the development of heart failure, MURC-knockout (KO) and wild-type (WT) mice were subjected to pressure overload by transverse aortic constriction (TAC). After TAC, WT mice developed overt heart failure and eccentric cardiac hypertrophy, whereas MURC-KO mice showed preserved cardiac function accompanied by attenuated cardiac hypertrophy. We then focused on β-adrenergic receptor (AR) signaling, because MURC was colocalized and associated with β1-AR. ...
TY - JOUR. T1 - CD44 regulates hepatocyte growth factor-mediated vascular integrity. T2 - Role of c-Met, Tiam1/Rac1, dynamin 2, and cortactin. AU - Singleton, Patrick A.. AU - Salgia, Ravi. AU - Moreno-Vinasco, Liliana. AU - Moitra, Jaideep. AU - Sammani, Saad. AU - Mirzapoiazova, Tamara. AU - Garcia, Joe GN. PY - 2007/10/19. Y1 - 2007/10/19. N2 - The preservation of vascular endothelial cell (EC) barrier integrity is critical to normal vessel homeostasis, with barrier dysfunction being a feature of inflammation, tumor angiogenesis, atherosclerosis, and acute lung injury. Therefore, agents that preserve or restore vascular integrity have important therapeutic implications. In this study, we explored the regulation of hepatocyte growth factor (HGF)-mediated enhancement of EC barrier function via CD44 isoforms. We observed that HGF promoted c-Met association with CD44v10 and recruitment of c-Met into caveolin-enriched microdomains (CEM) containing CD44s (standard form). Treatment of EC with ...
Caveolae are specialized membrane domains that function in endocytosis and contain relatively high concentrations of signaling molecules. Isshiki et al. now present evidence that caveolae serve as preferential sites of Ca2+ influx across the plasma membrane when intracellular stores of Ca2+ are depleted. The authors fused the calcium sensor yellow chameleon 3.1 to the COOH-terminal end of caveolin-1 to target the sensor to caveolae. Then, they compared Ca2+ signals at the caveolae to those from unmodified yellow chameleon in the cytoplasm or from another fusion protein that targeted the sensor to the plasma membrane. When Ca2+ was depleted from the endoplasmic reticulum of fetal bovine endothelial cells by exposure of the cells to adenosine triphosphate (ATP) and thapsigargin, Ca2+ entry into the cells occurred preferentially at caveolae, apparently through store-operated channels of the TRP (transient receptor potential) family. The authors propose that caveolae may function as preassembled, ...
article{364f9c3e-a101-48eb-bf5b-ea6e0c163acd, abstract = {,p,Cavins belong to a family of proteins that contribute to the formation of caveolae, which are membrane organelles with functional roles in muscle and fat. Here, we investigate the effect of cavin-3 ablation on vascular and urinary bladder structure and function. Arteries and urinary bladders from mice lacking cavin-3 (knockout: KO) and from controls (wild type: WT) were examined. Our studies revealed that the loss of cavin-3 resulted in ∼40% reduction of the caveolae protein cavin-1 in vascular and bladder smooth muscle. Electron microscopy demonstrated that the loss of cavin-3 was accompanied by a reduction of caveolae abundance by 40-45% in smooth muscle, whereas the density of caveolae in endothelial cells was unchanged. Vascular contraction in response to an α,sub,1,/sub,-adrenergic agonist was normal but nitric-oxide-dependent relaxation was enhanced, in parallel with an increased relaxation on direct activation of soluble ...
In biology, caveolae (Latin for little caves; singular, caveola), which are a special type of lipid raft, are small (50-100 nanometer) invaginations of the plasma membrane in many vertebrate cell types, especially in endothelial cells, adipocytes and embryonic notochord cells. They were originally discovered by E. Yamada in 1955. These flask-shaped structures are rich in proteins as well as lipids such as cholesterol and sphingolipids and have several functions in signal transduction. They are also believed to play a role in mechanoprotection, mechanosensation, endocytosis, oncogenesis, and the uptake of pathogenic bacteria and certain viruses. Formation and maintenance of caveolae was initially thought to be primarily due to caveolin, a 21 kD protein. There are three homologous genes of caveolin expressed in mammalian cells: Cav1, Cav2 and Cav3. These proteins have a common topology: cytoplasmic N-terminus with scaffolding domain, long hairpin transmembrane domain and cytoplasmic C-terminus. ...
The plasma membrane is not uniform, but can be divided into different microdomains. Whereas some microdomains, such as clathrin-coated pits, are morphologically identifiable, others can be identified only upon colocalization with domain-specific marker molecules. Caveolae are microdomains originally identified morphologically as small noncoated invaginations of the plasma membrane. The formation of caveolae depends on expression of caveolin and, since the plasma membrane contains different noncoated invaginations, caveolae can be identified only by labeling for caveolin. Some confusion exists with respect to the relationship between caveolae and lipid rafts [see raft nomenclature ( Simons and Toomre, 2000)]. This is partly due to difficulties in isolating pure caveolae and to the various methods used for this purpose. Initially, subcellular fractionation upon Triton X-100 extraction at 4°C was used. Such fractionation allows the isolation of detergent-resistant membranes [DRMs; also called DIGs ...
Virus tracking in living NMuMG epithelial cells expressing GFP-fused caveolin-1. (a) Caveolar endocytosis, fission and fusion events, and internal vesicle membrane dynamics are shown not to be affected in a stable cell line. (b) Detail of an infected cell (in a surface confocal section) with a virion bypassing a caveolin-rich domain. (c) In-depth confocal section with a virion(s) captured in a caveolin-rich vesicle at the nuclear periphery. (d and e) Uptake of virions through caveolin membrane domains at the cell surface. Mouse NMuMG epithelial cells expressing GFP-tagged caveolin-1 were infected with Alexa 594-prestained virus (MOI of 10e3 virus particles per cell) and scanned in an open, mediumcontaining chamber with a deltaT of 6 s. Bars, 5 um (a to c) and 2 um (d and e). Liebl et al., J Virol 2006. ...
This download caveolins was been, and received on the treatment by Sir Henry Irving in 1875. Harold was in 1876, The Cup in 1881( at the Lyceum), The Promise of May( at the Globe) in 1882, Becket in 1884, with The Foresters in 1892. The download is one from which I give, not right of secure single-player of the purchase and of of rendering for the analysis.
opamine D3 receptor, Caveolin1, Caveolin 1, 3 dopamine receptor, D, Dopamine receptor D3, DRD, ETM1, FET1, Cav-1, ETM1, CAV 1, CAV1, CAV1_HUMAN.
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The molecular composition of plasma membranes is constantly remodeled by endocytosis and exocytosis. Eisosomes are large cytoplasmic protein assemblies that localize to specialized domains on the yeast plasma membrane. They are of uniform size and immobile, and their disruption leads to large aberrant plasma membrane invaginations and endocytic defects. It is unknown how eisosomes are formed or inherited and what governs their size, distribution, and location. Here we show that eisosomes are formed de novo in the bud of dividing cells. They colonize newly formed membrane at a fixed density in a polarized wave proceeding from the bud neck to the bud tip and become anchored at the site of their formation. Pil1, one of the two main eisosome subunits, emerges as the central regulator of eisosome biogenesis that determines both size and location of eisosomes. Lowering Pil1 expression leads to normal-sized eisosomes at a reduced density, suggesting that eisosomes must be of a minimal size. Conversely, ...
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The primary targets of defense peptides are plasma membranes, and the induced irreversible depolarization is sufficient to exert antimicrobial activity although secondary modes of action might be at w
Introduction. Caveolin-1(Cav-1) is a multifunctional scaffolding protein essential structural constituent of caveolae, regulating multiple processes in cancer.. A growing body of evidence links elevated expression of Cav-1 to an aggressive phenotype of cancer cells and to a poor prognosis in several tumors. The molecular mechanisms underlying the pro-survival and tumor-promoting functions of Cav-1 and the down-stream mediators of Cav-1 functional role in metastatic cancer have jet to be fully identified. The aim of the present work was to prosecute the investigation on such molecular pathways in solid tumors, based on our recent data obtained in cell lines from SCLC (SCLC-R1) and NSCLC (RAL) and from glioblastoma (A-172) where we demonstrated an oncopromoter activity of Cav-1 through the activation of STAT3 signaling pathway. Here we present the data obtained in a cell line from osteosarcoma (143B), where Cav-1 was highly expressed.. Methods. Cav-1 was knocked-down by siRNA using the ...
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Cav-1 expression is negatively related with COL I expression in chronologically-aged human and mouse skinTo compare the age-dependent change of Cav-1 and COL I,
It interacts with caveolin-1. GRCh38: Ensembl release 89: ENSG00000144063 - Ensembl, May 2017 GRCm38: Ensembl release 89: ... 2001). "BENE, a novel raft-associated protein of the MAL proteolipid family, interacts with caveolin-1 in human endothelial- ... 36 (1): 40-5. doi:10.1038/ng1285. PMID 14702039. Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and ... "A novel gene encoding an SH3 domain protein is mutated in nephronophthisis type 1". Nat Genet. 17 (2): 149-53. doi:10.1038/ ...
Lu ML, Schneider MC, Zheng Y, Zhang X, Richie JP (April 2001). "Caveolin-1 interacts with androgen receptor. A positive ... Caveolin 1, CDK9, COX5B, CREB-binding protein, Cyclin D1, Cyclin-dependent kinase 7, DACH1, Death associated protein 6, L-DOPA ... 275 (1): 571-9. doi:10.1074/jbc.275.1.571. PMID 10617653. Liu Y, Kim BO, Kao C, Jung C, Dalton JT, He JJ (May 2004). "Tip110, ... 16 (1): 85-99. doi:10.1210/mend.16.1.0753. PMID 11773441. Pero R, Lembo F, Palmieri EA, Vitiello C, Fedele M, Fusco A, Bruni CB ...
Data from Caveolin-1 knockout mice demonstrated that TLR5 expression significantly decreases in the absence of Caveolin-1 ... It is hypothesized that the Caveolin-1 directly interacts with TLR5 to stabilize it and hence increases the level of TLR5. TLR5 ... Lim JS, Nguyen KC, Han JM, Jang IS, Fabian C, Cho KA (December 2015). "Direct Regulation of TLR5 Expression by Caveolin-1". ... Recent study has identified Caveolin-1 as a potential regulator of TLR5 expression. In contrast to the decreased TLR4 level in ...
PTGS2 has been shown to interact with caveolin 1. PTGS2 (COX-2) was discovered in 1991 by the Daniel Simmons laboratory at ... "Colocalization and interaction of cyclooxygenase-2 with caveolin-1 in human fibroblasts". J. Biol. Chem. 276 (37): 34975-82. ... 88 (1-2): 24-30. doi:10.1016/j.lfs.2010.10.017. PMC 3046773. PMID 21035466. Wang D, Patel VV, Ricciotti E, Zhou R, Levin MD, ... PTGS1 (COX-1) is constitutively expressed in many tissues and is the predominant form in gastric mucosa and in the kidneys. ...
"A functional interaction between sprouty proteins and caveolin-1". The Journal of Biological Chemistry. 281 (39): 29201-12. doi ... Protein sprouty homolog 1 is a protein that in humans is encoded by the SPRY1 gene. Neurofibromin 1 SPRED1 GRCh38: Ensembl ... 342 (1-2): 57-62. doi:10.1007/s11010-010-0468-8. PMC 2923832. PMID 20461448. SPRY1 human gene location in the UCSC Genome ... "Entrez Gene: SPRY1 sprouty homolog 1, antagonist of FGF signaling (Drosophila)". Lim J, Wong ES, Ong SH, Yusoff P, Low BC, Guy ...
Cabrita MA, Jäggi F, Widjaja SP, Christofori G (Sep 2006). "A functional interaction between sprouty proteins and caveolin-1". ... 287 (1): L52-9. doi:10.1152/ajplung.00430.2003. PMID 14977631. Sasaki A, Taketomi T, Kato R, Saeki K, Nonami A, Sasaki M, ...
Cabrita MA, Jäggi F, Widjaja SP, Christofori G (2006). "A functional interaction between sprouty proteins and caveolin-1". J. ... 36 (1): 40-5. doi:10.1038/ng1285. PMID 14702039. Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and ...
Endothelin receptor type B has been shown to interact with Caveolin 1. Agonists IRL-1620 Antagonists A-192,621 BQ-788 Bosentan ... Yamaguchi T, Murata Y, Fujiyoshi Y, Doi T (Apr 2003). "Regulated interaction of endothelin B receptor with caveolin-1". ... 178 (1): 248-55. doi:10.1016/0006-291X(91)91806-N. PMID 1648908. Cyr C, Huebner K, Druck T, Kris R (Nov 1991). "Cloning and ... 181 (1): 184-90. doi:10.1016/S0006-291X(05)81399-3. PMID 1659806. "Entrez Gene: EDNRB endothelin receptor type B". Sato-Jin K, ...
Caveolin-1 conversely inhibits ROMK channel activity, and expression of the two shows a clear inverse relationship. Co- ... MicroRNA Lin DH, Yue P, Pan C, Sun P, Wang WH (2011). "MicroRNA 802 stimulates ROMK channels by suppressing caveolin-1". J Am ... miR-802 expression in the kidney is stimulated by a high potassium intake, along with caveolin-1 expression, one of the many ... Lin, D. -H.; Yue, P.; Pan, C.; Sun, P.; Wang, W. -H. (2011). "MicroRNA 802 Stimulates ROMK Channels by Suppressing Caveolin-1 ...
... directly binds caveolin-1". FEBS Letters. 508 (1): 49-52. doi:10.1016/S0014-5793(01)03020-4. PMID 11707266. S2CID 7887096. ... 8 (1): 157-71. doi:10.1074/mcp.M800266-MCP200. PMC 2621004. PMID 18782753. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S ... 51 (1): 136-9. doi:10.1006/geno.1998.5342. PMID 9693043. Castets F, Bartoli M, Barnier JV, Baillat G, Salin P, Moqrich A, ... 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. v t e. ...
Schlegel A, Wang C, Pestell RG, Lisanti MP (Oct 2001). "Ligand-independent activation of oestrogen receptor alpha by caveolin-1 ... 16 (1): 128-40. doi:10.1210/mend.16.1.0755. PMID 11773444. DiRenzo J, Shang Y, Phelan M, Sif S, Myers M, Kingston R, Brown M ( ... 1 (4): 237-50. doi:10.1379/1466-1268(1996)001. 2.3.CO;2. PMC 376461. PMID 9222609. Lee MO, Kim EO, Kwon HJ, Kim YM, Kang HJ, ... 69 (1): 63-71. doi:10.1006/geno.2000.6299. PMID 11013076. Hu YC, Shyr CR, Che W, Mu XM, Kim E, Chang C (Sep 2002). "Suppression ...
... directly binds caveolin-1". FEBS Lett. 508 (1): 49-52. doi:10.1016/S0014-5793(01)03020-4. PMID 11707266. S2CID 7887096. Suzuki ... 8 (1): 157-71. doi:10.1074/mcp.M800266-MCP200. PMC 2621004. PMID 18782753. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S ... 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. Goudreault M, D'Ambrosio LM, Kean MJ, Mullin MJ, Larsen BG, ... 200 (1-2): 149-56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. Maruyama K, Sugano S (1994). "Oligo-capping: a simple ...
2007). "The tetraspan protein EMP2 regulates expression of caveolin-1". J. Biol. Chem. 282 (36): 26542-51. doi:10.1074/jbc. ... 183 (1-2): 69-75. doi:10.1016/S0378-1119(96)00475-1. PMID 8996089. Liehr T, Kuhlenbaumer G, Wulf P, Taylor V, Suter U, Van ... 58 (1): 106-8. doi:10.1006/geno.1999.5803. PMID 10331954. Wadehra M, Forbes A, Pushkarna N, Goodglick L, Gordon LK, Williams CJ ... 175 (1-2): 115-20. doi:10.1016/0378-1119(96)00134-5. PMID 8917086. Street VA, Goldy JD, Golden AS, et al. (2002). "Mapping of ...
Thus, micro103/107 may affect insulin sensitivity by targeting Caveolin-1. The blockmir CD5-2 has been shown to inhibit the ... It has been shown that Caveolin-1-deficient mice show insulin resistance. MicroRNA-103/107 inhibition in Caveolin-1-deficient ... This may be achieved by designing a Blockmir that matches seed 1. microRNA-33a/b MicroRNA-33a/b inhibition in mice leads to ...
"Functional interaction of caveolin-1 with Bruton's tyrosine kinase and Bmx". J. Biol. Chem. 277 (11): 9351-7. doi:10.1074/jbc. ... Phase 1: ONO-4059 for non-Hodgkin lymphoma and/or CLL. Renamed GS-4059 and now in trial NCT02457598. Spebrutinib (AVL-292, CC- ... 228 (1): 42-53. doi:10.1016/j.cellimm.2004.03.004. PMID 15203319. Yasuda T, Tezuka T, Maeda A, Inazu T, Yamanashi Y, Gu H, ... 461 (1-2): 68-72. doi:10.1016/S0014-5793(99)01424-6. PMID 10561498. Matsushita M, Yamadori T, Kato S, Takemoto Y, Inazawa J, ...
"Interaction of the immunoglobulin-like cell adhesion molecule hepaCAM with caveolin-1". Biochemical and Biophysical Research ... 37 (1): 155-65. doi:10.3892/ijo_00000663. PMID 20514407. Moh MC, Shen S (2009). "The roles of cell adhesion molecules in tumor ... In cell signaling, hepaCAM directly interacts with F-actin and calveolin 1, and is capable of inducing senescence-like growth ...
Caveolin 1 has been shown to interact with Nitric oxide synthase 2A. and Rac2. Autosomal recessive NOS2 deficiency has been ... Felley-Bosco E, Bender FC, Courjault-Gautier F, Bron C, Quest AF (December 2000). "Caveolin-1 down-regulates inducible nitric ... occurring in fewer than 1 per million persons. GRCh38: Ensembl release 89: ENSG00000007171 - Ensembl, May 2017 GRCm38: Ensembl ...
March 1999). "Phospholipase D1 in caveolae: regulation by protein kinase Calpha and caveolin-1". Biochemistry. 38 (12): 3763-9 ... 302 (1): 127-32. doi:10.1016/s0006-291x(03)00112-8. PMID 12593858. Cai S, Exton JH (May 2001). "Determination of interaction ... 168 (1): 233-40. PMID 20291081. Jenkins GM, Frohman MA (October 2005). "Phospholipase D: a lipid centric review". Cellular and ... July 1998). "Activation of phospholipase D1 by direct interaction with ADP-ribosylation factor 1 and RalA". FEBS Letters. 430 ( ...
2002). "Functional interaction of caveolin-1 with Bruton's tyrosine kinase and Bmx". J. Biol. Chem. 277 (11): 9351-7. doi: ... doi:10.1016/S1570-9639(02)00524-1. PMID 12573241. Zhang R, Xu Y, Ekman N, et al. (2004). "Etk/Bmx transactivates vascular ...
High levels of caveolin Cav1 are expressed in adipocytes. Caveolin associates with cholesterol, fatty acids and lipid droplets ... The presence of caveolin leads to a local change in morphology of the membrane. Cavin proteins emerged in the late 2000s to be ... Formation and maintenance of caveolae was initially thought to be primarily due to caveolin, a 21 kD protein. There are three ... However, some bacteria do not use typical caveolae but only caveolin-rich areas of the plasma membrane. The pathogens ...
Vargas L, Nore BF, Berglof A, Heinonen JE, Mattsson PT, Smith CI, Mohamed AJ (March 2002). "Functional interaction of caveolin- ... Phase 1: *ONO-4059 for Non-Hodgkin's Lymphoma and/or CLL.[9] Renamed GS-4059 and now in trial NCT02457598.[10] ... doi:10.1016/S0006-2952(98)00122-1. PMID 9751072.. *. Tsubata T, Wienands J (2001). "B cell signaling. Introduction". Int. Rev. ... BeiGene Announces Initiation of a Combination Trial of the BTK Inhibitor BGB-3111 with the PD-1 Antibody BGB-A317. June 2016 ...
... has been shown to interact with Caveolin 1 and peroxisomal receptor PEX5. GRCh38: Ensembl release 89: ENSG00000116171 - ... "Sterol carrier protein-2 directly interacts with caveolin-1 in vitro and in vivo". Biochemistry. 43 (23): 7288-306. doi:10.1021 ... 76 (1): 73-84. doi:10.1016/0009-3084(95)02436-M. PMID 7788802. Vesa J, Hellsten E, Barnoski BL, et al. (1994). "Assignment of ... 335 (1): 18-26. doi:10.1016/0014-5793(93)80431-S. PMID 8243660. S2CID 9969358. Seedorf U, Scheek S, Engel T, et al. (1994). " ...
This gene codes for the Caveolin protein, which is a scaffolding membrane protein. This protein plays a role in lipid ... April 2008). "Association of a Homozygous Nonsense Caveolin-1 Mutation with Berardinelli-Seip Congenital Lipodystrophy". ... In type 1 patients, they still have mechanical adipose tissue, but type 2 patients do not have any adipose tissue, including ... The gene for type 1 CGL was identified as AGPAT2 at chromosome 9q34, and later the gene for type 2 CGL was identified as BSCL2 ...
Caveolin 1 is dephosphorylated on tyrosine 14 in response to shear stress and PTPmu is hypothesized to catalyze this reaction. ... Caveolin 1 is a scaffolding protein enriched in endothelial cell junctions that is also linked to shear stress regulated ... Shin J, Jo H, Park H (2006). "Caveolin-1 is transiently dephosphorylated by shear stress-activated protein tyrosine phosphatase ... 248 (1): 329-38. doi:10.1006/excr.1999.4428. PMID 10094839. Koop EA, Lopes SM, Feiken E, Bluyssen HA, van der Valk M, Voest EE ...
Caveolin 1, Tight junction protein 1 CSNK1D, and PTPmu (PTPRM). Connexin Hypoplastic left heart syndrome GRCh38: Ensembl ... "Connexin family members target to lipid raft domains and interact with caveolin-1". Biochemistry. 41 (18): 5754-5764. doi: ... Gap junction alpha-1 protein (GJA1), also known as connexin 43 (Cx43), is a protein that in humans is encoded by the GJA1 gene ... 58 (1): 34-40. doi:10.1006/geno.1999.5814. PMID 10331943. Fishman GI, Eddy RL, Shows TB, Rosenthal L, Leinwand LA (May 1991). " ...
"Connexin family members target to lipid raft domains and interact with caveolin-1". Biochemistry. 41 (18): 5754-64. doi:10.1021 ... ISBN 978-1-934115-46-6. Hsieh CL, Kumar NM, Gilula NB, Francke U (1991). "Distribution of genes for gap junction membrane ... GJA3 has been shown to interact with Tight junction protein 1. GRCh38: Ensembl release 89: ENSG00000121743 - Ensembl, May 2017 ... 2004). "Lens connexins alpha3Cx46 and alpha8Cx50 interact with zonula occludens protein-1 (ZO-1)". Mol. Biol. Cell. 14 (6): ...
Caveolin-1-mediated STAT3 activation determines electrotaxis of human lung cancer cells". Oncotarget. 9 (46): 28291. doi: ... 56 (1): 18-27. doi:10.1108/00035590910923428. ISSN 0003-5599. Bastos, A. C.; Quevedo, M. C.; Karavai, O. V.; Ferreira, M. G. S ... 157 (1-3): 193-202. doi:10.1007/BF01322652. ISSN 0033-183X. S2CID 38050334. Lee, Joon Sang (2006). "Response to red and blue ... Berger, François; Delhalle, Joseph; Mekhalif, Zineb (2009). "Undec-10-ene-1-thiol multifunctional molecular layer as a junction ...
Lisanti MP, Scherer PE, Tang Z, Sargiacomo M (Jul 1994). "Caveolae, caveolin and caveolin-rich membrane domains: a signalling ... Ju H, Zou R, Venema VJ, Venema RC (Jul 1997). "Direct interaction of endothelial nitric-oxide synthase and caveolin-1 inhibits ... the enzyme is inactivated due to the strong and direct interaction of eNOS with caveolin-1. The binding of calcium-activated ... 267 (5 Pt 1): C1405-13. doi:10.1152/ajpcell.1994.267.5.C1405. PMID 7977701. Ignarro LJ, Buga GM, Wei LH, Bauer PM, Wu G, del ...
"Colocalization and interaction of cyclooxygenase-2 with caveolin-1 in human fibroblasts". J. Biol. Chem. 276 (37): 34975-82. ... 13 (1): 45-82. PMID 10223523.. *^ Mulugeta S, Suzuki T, Hernandez NT, Griesser M, Boeglin WE, Schneider C (2010). " ... 66 (1): 13-8. PMID 12051953. doi:10.1054/plef.2001.0335.. *. Saukkonen K, Rintahaka J, Sivula A, Buskens CJ, Van Rees BP, Rio ... 23 (1-2): 63-75. PMID 15000150. doi:10.1023/A:1025863029529.. *. Jain S, Khuri FR, Shin DM (2004). "Prevention of head and neck ...
Caveolin-1 Influences LFA-1 Redistribution upon TCR Stimulation in CD8 T Cells (2017) Proximity of TCR and its CD8 coreceptor ... "Caveolin-1 Influences LFA-1 Redistribution upon TCR Stimulation in CD8 T Cells". Journal of Immunology. 199 (3): 874-884. doi: ... investigating the role of Caveolin-1 in T cell cholesterol homeostasis, integrin signaling and exosome complex biogenesis. In ... 157 (1-2): 16-22. doi:10.1016/j.imlet.2013.11.005. ISSN 0165-2478. PMC 3931270. PMID 24263053. Libri, Valentina; Helwak, ...
1) Alpha-gamma coactivation. Here it is posited that gamma motoneurons are activated in parallel with alpha motoneurons to ... Muscle spindles are composed of 5-14 muscle fibers, of which there are three types: dynamic nuclear bag fibers (bag1 fibers), ... The dynamic axons innervate the bag1 intrafusal muscle fibers. They increase the stretch-sensitivity of the Ia afferents by ... static nuclear bag fibers (bag2 fibers), and nuclear chain fibers.[1][2] ...
Yamamoto H, Komekado H, Kikuchi A (2006). "Caveolin is necessary for Wnt-3a-dependent internalization of LRP6 and accumulation ...
7 (1): 32-44. doi:10.1038/nrm1786. PMID 16493411.. *. Dutta D, Williamson CD, Cole NB, Donaldson JG (Sep 2012). "Pitstop 2 is a ... "Epsin 1 EM gallery". MRC Laboratory of Molecular Biology,. Archived from the original on 2009-01-02. Retrieved 2009-04-17. ... Clathrin was first isolated and named by Barbara Pearse in 1976.[1] It forms a triskelion shape composed of three clathrin ... Eukaryotic Linear Motif resource motif class LIG_Clathr_ClatBox_1. *Eukaryotic Linear Motif resource motif class LIG_Clathr_ ...
"Amyloid β-Protein Stimulates Trafficking of Cholesterol and Caveolin-1 from the Plasma Membrane to the Golgi Complex in Mouse ... Neurosci. 1 (5): 355-8. PMID 10196523. doi:10.1038/1565.. *↑ Roses AD (February 1998). "Alzheimer diseases: a model of gene ... 20,0 20,1 Zhao LN, Long H, Mu Y, Chew LY (2012). "The Toxicity of Amyloid β Oligomers". Int J Mol Sci 13 (6): 7303-27. PMC ... 62,0 62,1 62,2 62,3 62,4 Kang JE, Lim MM, Bateman RJ, Lee JJ, Smyth LP, Cirrito JR, Fujiki N, Nishino S, Holtzman DM (November ...
1] The overall comprehensive organization of the perimysium collagen network, as well as its continuity and disparateness, ...
Caveolin is paradoxically tumour promoting. NF-κB pathway activation overexpression may contribute to the inflammatory ... caveolin-1 and -2 are overexpressed and may contribute to tumour cell motility[13] ... RhoC GTPase is overexpressed, possibly related to overexpression (hypomethylation) of caveolin-1 and -2. ... "Overexpression of caveolin-1 and -2 in cell lines and in human samples of inflammatory breast cancer". Breast Cancer Research ...
Elastic filaments, 1 nm in diameter, are made of titin, a large springy protein. They run through the core of each thick ... Myofilaments are the filaments of myofibrils, constructed from proteins,[1] principally myosin or actin. Types of muscle are ...
... caveolin, dynamin and actin. Once within the cell the viruses are rapidly delivered to endosomes via vesicular trafficking ... Lassa virus (LASV) is an arenavirus that causes Lassa hemorrhagic fever,[1] a type of viral hemorrhagic fever (VHF), in humans ... Frame JD, Baldwin JM, Gocke DJ, Troup JM (1 July 1970). "Lassa fever, a new virus disease of man from West Africa. I. Clinical ... It has been reported that the cellular protease SKI-1/S1P is responsible for this cleavage. The cleaved glycoproteins are ...
"Membrane Localization, Caveolin-3 Association and Rapid Actions of Vitamin D Receptor in Cardiac Myocytes". Steroids 75 (8-9 ... 11,0 11,1 11,2 11,3 11,4 Kitagawa H, Fujiki R, Yoshimura K, Mezaki Y, Uematsu Y, Matsui D, Ogawa S, Unno K, Okubo M, Tokita A, ... O receptor da vitamina D (VDR) ou receptor do calcitriol, tamén chamado NR1I1 (receptor nuclear familia 1, grupo I, membro 1), ... 13,0 13,1 13,2 Ito M, Yuan CX, Malik S, Gu W, Fondell JD, Yamamura S, Fu ZY, Zhang X, Qin J, Roeder RG (March 1999). "Identity ...
38 (1): 69-78. doi:10.1016/S0896-6273(03)00151-X.. *^ Cesca et al. (2010) The synapsins: Key actors of synapse function and ... 17-1-17-41. doi:10.1201/9781420008142.ch17 (inactive 2019-06-07). ISBN 978-0-8493-8528-5. . Archived from the original on 2016- ... The cycle begins with (1) a region of the golgi apparatus is pinched off to form the synaptic vesicle and this vesicle is ... 2010). "Intersectin 1 forms complexes with SGIP1 and Reps1 in clathrin-coated pits". Biochemical and Biophysical Research ...
... localizes in a lipid raft/caveola and inhibits ERK activation in collaboration with caveolin-1". Genes to Cells. 10 (9): 887-95 ... Sprouty-related, EVH1 domain-containing protein 1 (Spread-1) is a protein that in humans is encoded by the SPRED1 gene located ... Neurofibromin 1. *Patients without Neurofibromin 1 or SPRED1 mutations may have SPRED2, SPRED3 or SPRY1, SPRY2, SPRY3 or SPRY4 ... Spread-1 is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth ...
Heritable (BMPR2, ALK1, SMAD9, caveolin 1, KCNK3 mutations). *Drug- and toxin-induced (e.g., methamphetamine use [23] ) ... 37 (1): 67-119. doi:10.1093/eurheartj/ehv317. PMID 26320113.. *^ a b c d e f g h i j k l McLaughlin VV, Archer SL, Badesch DB, ... 44 (1): 1-10. doi:10.1165/rcmb.2009-0388TR. PMID 20448055.. *^ a b c d e f Wood SF (January 1986). "Astemizole and terfenadine ... 978-0-07-144874-1. .. *^ Ghosh, Amit K. (2008). Mayo Clinic Internal Medicine Review: Eighth Edition (Mayo Clinic Internal ...
Yamamoto, M; Toya Y; Jensen R A; Ishikawa Y (March 1999). "Caveolin is an inhibitor of platelet-derived growth factor receptor ... 1-50. CD1 (a-c, 1A, 1D, 1E) • CD2 • CD3 (γ, δ, ε) • CD4 • CD5 • CD6 • CD7 • CD8 (a) • CD9 • CD10 • CD11 (a, b, c) • CD13 • CD14 ... CD201 • CD202b • CD204 • CD205 • CD206 • CD207 • CD208 • CD209 • CDw210 (a, b) • CD212 • CD213a (1, 2) • CD217 • CD218 (a, b) ... PDGFRA formira interakcije sa PDGFRB,[2][3] PLCG1,[4] Natrijum-vodonik antiporter 3 regulator 1,[5] Cbl gene,[6] CRK,[7][8] ...
"Chapter 1: The Muscle Spindle and the Central Nervous System". Neuromuscular Reeducation with Electromyometric Feedback (PDF). ... 1] They constitute the muscle spindle and are innervated by both sensory (afferent) and motor (efferent) fibers. Gamma ...
Although receptors and their ligands can be brought into the cell through a few mechanisms (e.g. caveolin), clathrin-mediated ... Budding of the plasma membrane then occurs, forming a clathrin coated pit.[1] Other receptors can nucleate a clathrin-coated ...
Frank P, Lisanti M (2004). "Caveolin-1 and caveolae in atherosclerosis: differential roles in fatty streak formation and ... ISBN 9958-9344-1-8. *^ Parton, R.G. and K. Simons, The multiple faces of caveolae. Nature Reviews Molecular Cell Biology, 2007 ... 1] Vjeruje se da imaju važnu ulogu u endocitozi, onkogenezi i preuzimanju patogenih bakterije i određenih virusa.[2][3][4] ...
It is typically used to describe the contraction properties of pennate muscles.[1] It is not the same as the anatomical cross- ... is always smaller than 1, PCSA2 is always smaller than PCSA. Hence, it cannot be described as the total area of the cross- ... Figure 1 Pennate muscle fiber arrangements. The green lines represent PCSA; the blue lines represent ACSA ... The muscle cross-sectional area (blue line in figure 1, also known as anatomical cross-section area, or ACSA) does not ...
396 (Pt 1): e1-3. doi:10.1042/BJ20060377. ISSN 0264-6021. PMC 1450001. PMID 16626281.. ... T-tubules in skeletal muscle are associated with two terminal cisternae, known as a triad.[1][6] ... BIN-1)". Journal of the American Heart Association. 6 (5). doi:10.1161/JAHA.116.004834. ISSN 2047-9980. PMC 5524063. PMID ... 1] Due to this complex orientation, some refer to T-tubules as the transverse-axial tubular system.[2] The inside or lumen of ...
A myocyte (also known as a muscle cell)[1] is the type of cell found in muscle tissue. Myocytes are long, tubular cells that ... ISBN 978-0-07-337825-1.. *^ Sugi, Haruo; Abe, T; Kobayashi, T; Chaen, S; Ohnuki, Y; Saeki, Y; Sugiura, S; Guerrero-Hernandez, ... 267 (6 Pt 1): C1723-8. PMID 7545970.. *^ Pette, D; Staron, RS (Sep 15, 2000). "Myosin isoforms, muscle fiber types, and ... Bentzinger, CF; Wang, YX; Rudnicki, MA (1 February 2012). "Building muscle: molecular regulation of myogenesis". Cold Spring ...
"Membrane Localization, Caveolin-3 Association and Rapid Actions of Vitamin D Receptor in Cardiac Myocytes". Steroids 75 (8-9 ... Receptor kalcitriola - poznat i kao receptor vitamina D (VDR) i NR1 I 1 (potporodica nuklearnih receptora 1, grupa I, član 1 ... "Entrez Gene: VDR vitamin D (1,25- dihydroxyvitamin D3) receptor". *^ Luderer HF, Demay MB (July 2010). "The vitamin D receptor ... je član porodice nuklearnih receptora transkripcijskih faktora.[1] Nakon aktivacije vitamina D, VDR formira heterodimer sa ...
Caveolin 3. *Collagen, type I, alpha 2. *Complement component 1r. *Complement component 1s ... 13:54, 1 August 2017. 453 × 200 (10.31 MB). Was a bee. {{Information ,Description={{en,1=Ideogram of house mouse (''Mus ...
Lu, Zhimin; Ghosh, Sourav; Wang, Zhiyong; Hunter, Tony (December 2003). "Downregulation of caveolin-1 function by EGF leads to ... 56 (1): 3-7. doi:10.2337/db06-1165. PMID 17110468.. *^ Morton RA, Geras-Raaka E, Wilson LM, et al. (2007). "Endocrine precursor ... 0: 1-17. doi:10.1080/19336918.2018.1471323. PMID 29781749.. *^ Kajiyama H, Shibata K, Terauchi M, Yamashita M, Ino K, Nawa A, ... 201 (1): 27-36. doi:10.1677/joe-08-0497. PMID 19171567.. *^ Jolly, Mohit Kumar; Boareto, Marcelo; Huang, Bin; Jia, Dongya; Lu, ...
... is a coatomer, a protein complex[1] that coats vesicles transporting proteins from the cis end of the Golgi complex back ... 142 (1): 123-132. doi:10.1016/j.cell.2010.05.030. ISSN 1097-4172. PMC 2943847. PMID 20579721.. ...
211 (1): 82-96. doi:10.1111/apha.12212. PMID 24319999.. *^ Xu, Q; Quan, Y; Yang, L; He, J (Jan 2013). "An adaptive algorithm ... 21 (1): 65-73. doi:10.1109/TNSRE.2012.2226916. PMID 23193462.. *^ Milder, DA; Sutherland, EJ; Gandevia, SC; McNulty, PA (2014 ... 32 (1-2): 35-40. PMID 1541245.. *^ [1], Peak Performance - Endurance training: understanding your slow twitch muscle fibres ... It is a form of striated muscle tissue, which is under the voluntary control of the somatic nervous system.[1] Most skeletal ...
... s (/ˈmaɪəsɪn, -oʊ-/[1][2]) are a superfamily of motor proteins best known for their roles in muscle contraction and in a ... 51 (1): 1-15. doi:10.1002/cm.10014. PMID 11810692.. *^ von der Ecken J, Heissler SM, Pathan-Chhatbar S, Manstein DJ, Raunser S ... 46 (1): 61-7. doi:10.1002/ajmg.1320460110. PMID 7684189.. *^ "Archived copy". Archived from the original on 2009-07-07. ... 13 (1): 13-26. doi:10.1038/nrm3248. PMID 22146746. S2CID 11853457.. *^ Zimmermann D, Santos A, Kovar DR, Rock RS (August 2015 ...
... caveolin-2, and caveolin-3, respectively. All three members are membrane proteins with similar structure. Caveolin forms ... The caveolin gene family has three members in vertebrates: CAV1, CAV2, and CAV3, coding for the proteins caveolin-1, ... The expression pattern of caveolin-2 is similar to that of caveolin-1; it seems to be co-expressed with caveolin-1. ... and expression of caveolin-2 defines a caveolin gene family". Proc. Natl. Acad. Sci. U.S.A. 93 (1): 131-5. doi:10.1073/pnas. ...
60 (1): 90-142. doi:10.1152/physrev.1980.60.1.90. PMID 6766557.. *^ Kandel, Eric (2013). Principles of Neural Science, 5th ed. ... 90 (1): 345-57. doi:10.1152/jappl.2001.90.1.345. PMID 11133928.. *^ Karpati, George (2010). Disorders of Voluntary Muscle (PDF) ... A motor unit is made up of a motor neuron and the skeletal muscle fibers innervated by that motor neuron's axonal terminals.[1] ... Buchtal, F; H. Schmalbruch (1 January 1980). "Motor Unit of Mammalian Muscle". Physiological Reviews. ...
... caveolin 3) ... alpha 1) · β1(英语:SNTB1) · β2(英语:SNTB2) · γ1(英语:SNTG1) · γ2(英语: ... 肌肉組織(Muscle tissue)是組成動物身體肌肉的軟組織,肌肉的收縮是透過肌肉組織進行的。這和肌肉中其他的組織(例如腱或肌束膜)相反。肌肉組織是在胚胎發育時藉由肌發生(英语:myogenesis)(myogenesis)的過程而形成的[1
120 (Pt 1): 45-54. doi:10.1242/jcs.03302. PMID 17148574.. *^ Eaton S (Jan 2008). "Retromer retrieves wntless". Developmental ... 2014 Feb 1;6(2). pii: a016774. doi: 10.1101/cshperspect.a016774. "Retromer: a master conductor of endosome sorting". ... It is also required for the recycling of the cell surface receptor CED-1, which is necessary for phagocytosis of apoptotic ... 1) Cargo recognition complex that consist of Vps35, Vps29 and Vps26 (Vps trimer), and (2) SNX-BAR dimers which consist of SNX1 ...
All these interactions are through a caveolin-scaffolding domain (CSD) within caveolin-1 molecule. Molecules that interact with ... caveolin. Caveolin binding negatively regulates the auto-activation of Src tyrosine kinases". The Journal of Biological ... Caveolin-1 is a protein that in humans is encoded by the CAV1 gene. The scaffolding protein encoded by this gene is the main ... Caveolin 1 has been shown to interact with heterotrimeric G proteins, Src tyrosine kinases (Src, Lyn) and H-Ras,cholesterol,TGF ...
Caveolin-1 and Vascular Dysfunction. Thank you for your interest in the investigators Blood Pressure Research Study. The ... Caveolin-1 and Vascular Dysfunction. This study is ongoing, but not recruiting participants. ... Genetic Variation at the Caveolin-1 Locus [ Time Frame: 5 years ]. We are testing our hypothesis that genetic variation at the ... We will recruit hypertensive subjects who will be classified into two groups according to genotype status at rs926198 of CAV-1 ...
... Cancer Lett. 2006 Feb 20;233(1):10-5. doi: 10.1016/j. ... Conversely, dysregulation of caveolin-1 has been associated with several human diseases including multiple myeloma, an ... In this mini-review, we characterize the functional role of caveolin-1 in multiple myeloma, and present the preclinical ... 1 Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Jerome Lipper Multiple Myeloma Center, ...
Our results indirectly indicate that CAV-1 mediates the negative effects of PGC-1α on hepatic mitochondrial respiratory chain ... Furthermore, the levels of ROS modulator 1 (Romo1) and superoxide dismutase (SOD)-2, as well as catalase activity were ... Serum biochemistry, CAV-1 distribution, neutral lipid distribution, mitochondrial morphology, biogenesis-mediated protein ... The high levels of neutral lipids, malondialdehyde, peroxisome proliferator-activated receptor-γ coactive 1α (PGC-1α), and ...
Caveolin-1 Regulation of Breast Cancer Growth and Metastasis Lead researcher. Dr R L Anderson ...
In our study, we found that the tumor suppressor caveolin-1 (Cav1) is reduced upon infection with H. pylori, and CagA was ... Loss of Cav1 was caused by H. pylori-dependent activation of sterol-responsive element-binding protein-1 (SREBP1), and this ... event abolished the interaction of Cav1 with p120 RhoGTPase-activating protein/deleted in liver cancer-1 (p120RhoGAP/DLC1), a ... Li S, Seitz R, Lisanti MP (1996) Phosphorylation of caveolin by src tyrosine kinases. The alpha-isoform of caveolin is ...
IPR001612, Caveolin. IPR018361, Caveolin_CS. PANTHERi. PTHR10844, PTHR10844, 1 hit. PTHR10844:SF18, PTHR10844:SF18, 1 hit ... caveolin-mediated endocytosis Source: Ensembl. *cell differentiation Source: GO_CentralInferred from biological aspect of ... Belongs to the caveolin family.Curated. Phylogenomic databases. evolutionary genealogy of genes: Non-supervised Orthologous ... sp,A0M8S7,CAV1_FELCA Caveolin-1 OS=Felis catus OX=9685 GN=CAV1 PE=3 SV=1 ...
... Molecular Pathway in Intracerebral Hemorrhage of Rats. ... expression of caveolin-1, and activity of matrix metalloproteinase-2/9 at 6 h, 1 d, 3 d, and 7 d after ICH (P. ... in situ immunofluorescent detection of caveolin-1 in brains, western blot analysis of caveolin-1 in brains, and in situ ... exerts neuroprotection for acute ICH possibly via the caveolin-1/matrix metalloproteinase/blood-brain barrier permeability ...
Mouse monoclonal Caveolin-1 antibody [AT4C1]. Validated in WB, ICC/IF and tested in Mouse, Human. Immunogen corresponding to ... Anti-Caveolin-1 antibody [AT4C1] (ab265798) at 1/1000 dilution + Mouse heart tissue lysate at 40 µg. Secondary. Goat anti-mouse ... A549 (human lung carcinoma cell line) cells labeled for Caveolin-1 using ab265798 at 1/100 dilution, followed by an AlexaFluor® ... Recombinant fragment corresponding to Human Caveolin-1 aa 1-104.. Database link: Q03135 ...
... unveils the essential role of the protein caveolin-1 in a fundamental process for liver cure after injury or transplant. ... Fatty acids and caveolin-1 are essential in liver regeneration. A research published in Science by Catalan and Australian ... IDIBAPS researchers studied the role of caveolin-1 in this process, comparing the regenerative capacity of normal mice and ... Genetically modified mice, not expressing caveolin-1, were incapable of forming the lipidic bodies necessary in order to ...
Caveolin 1 Polyclonal Antibody from Invitrogen for Immunohistochemistry (Paraffin) applications. This antibody reacts with ... Supplied as 50 ug purified antibody (1 mg/ml) in PBS with , 0.1% sodium azide. ... Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene ... The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle ...
The lollipop plot above illustrates recurrent (observed in 3 or more out of 4440 TCGA tumor samples from 15 cancer types) and therefore potentially oncogenic missense mutations (click on Show Cancer Mutations). The bar plot below shows the proportion of tumor samples that have any kind of altering mutation(s) in the given protein. ...
Scherer, Z. Tang, and M. Sargiacomo, "Caveolae caveolin and caveolin-rich membrane domains: a signalling hypothesis," Trends in ... C.-F. Chang, S.-F. Chen, T.-S. Lee, H.-F. Lee, S.-F. Chen, and S.-K. Shyue, "Caveolin-1 deletion reduces early brain injury ... S. Fu, Y. Gu, J.-Q. Jiang et al., "Calycosin-7-O-β-d-glucoside regulates nitric oxide /caveolin-1/matrix metalloproteinases ... P. E. Scherer, R. Y. Lewis, D. Volonté et al., "Cell-type and tissue-specific expression of caveolin-2. Caveolins 1 and 2 co- ...
... caveolin-2 and caveolin-3 in thyroid cancer and stroma," Pathobiology, vol. 79, no. 1, pp. 1-10, 2012. View at Publisher · View ... Stromal Caveolin-1 and Caveolin-2 Expression in Primary Tumors and Lymph Node Metastases. Wladimir Gerstenberger,1 Michaela ... X. Ma, L. Liu, W. Nie et al., "Prognostic role of caveolin in breast cancer: a meta-analysis," The Breast, vol. 22, no. 4, pp. ... Caveolin 1 Expression in the Stroma of Lymph Node Metastasis. The CAV1 expression in stromal cells was also analyzed in 39 ...
Caveolin-1 is essential for both, Siddiqui et al. show.. Researchers already knew that caveolin-1 was necessary for ... Loss of caveolin-1 activated the enzyme eNOS, which spawns nitric oxide (NO) that reacts to form peroxynitrite. In turn, ... Previous work showed that adherens junctions become permeable in mice lacking caveolin-1, suggesting that the protein helps ... dissected the molecular chain of events that connects caveolin-1 to adherens junction integrity. ...
"Caveolin 1" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Caveolin-1 selectively regulates microRNA sorting into microvesicles after noxious stimuli. J Exp Med. 2019 09 02; 216(9):2202- ... Caveolin-1 scaffolding domain peptide prevents hyperoxia-induced airway remodeling in a neonatal mouse model. . 2019 07 01; 317 ... This graph shows the total number of publications written about "Caveolin 1" by people in Harvard Catalyst Profiles by year, ...
Invitrogen Anti-Caveolin 1 Polyclonal, Catalog # PA5-32691. Tested in Immunohistochemistry (Paraffin) (IHC (P)) applications. ... Caveolin-1 is also involved in the co-stimulatory signal essential for T-cell receptor (TCR) mediated T-cell activation. It can ... Cite Caveolin 1 Polyclonal Antibody. The following antibody was used in this experiment: Caveolin 1 Polyclonal Antibody from ... Caveolin-1 or CAV1 is a scaffolding proteins within caveolar membranes. It interacts directly with G-protein alpha subunits and ...
Caveolin-1+ve CL1-5 cells (B), C6 cells (C), and C7-Dox(−) cells (D) showed abundant filopodia formation. The caveolin-1−ve CL1 ... The caveolin-1−ve CL1E-9 cells were transfected with plasmid harboring a full-length human caveolin-1 encoding cDNA under the ... A: Expression of caveolin-1 protein in BEAS-2B cells, CL1-5, and CL1-5F4 cells, but not in CL1-0 and CL1-1 cells. Both the α- ... D and E: The growth rates of caveolin-1+ve C6 and C7 cells (with or without tetracycline treatment) compared with those of mock ...
2013) Caveolin isoform switching as a molecular, structural, and metabolic regulator of microglia. Mol Cell Neurosci 56:283-297 ... 2001) Caveolin-1 null mice are viable but show evidence of hyperproliferative and vascular abnormalities. J Biol Chem 276:38121 ... Caveolin-1 (Cav-1) is a major structural protein of caveolae found in cell membranes and is critical for numerous cellular ... Caveolin-1 (Cav-1) is a major structural protein of caveolae found in cell membranes that plays critical roles in numerous ...
Rabbit Monoclonal Anti-Caveolin-1 Antibody (SP43). Caveolae Marker, Endosome Marker. Validated: IHC, IHC-P. Tested Reactivity: ... Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene ... FAQs for Caveolin-1 Antibody (NBP1-49807). (Showing 1 - 1 of 1 FAQ).. * Does the SP43 antibody recognize Cav2? And Cav1 Beta ... Caveolin-1 Antibody (SP43) Summary. Immunogen. Synthetic peptide corresponding to C-terminus of human Caveolin 1 protein ...
Mouse Monoclonal Anti-Caveolin-1 Antibody (7C8) [Allophycocyanin]. Caveolae Marker, Endosome Marker. Validated: Flow. Tested ... FAQs for Caveolin-1 Antibody (NB100-615APC) (0). There are no specific FAQs related to this product. Read our general customer ... Reviews for Caveolin-1 Antibody (NB100-615APC) (0) There are no reviews for Caveolin-1 Antibody (NB100-615APC). By submitting a ... Blogs on Caveolin-1. There are no specific blogs for Caveolin-1, but you can read our latest blog posts. ...
PubMed journal article Caveolin-1-mediated STAT3 activation determines electrotaxis of human lung cancer cell were found in ... Caveolin-1-mediated STAT3 activation determines electrotaxis of human lung cancer cells.. Oncotarget 2017; 8(56):95741-95754O ... TY - JOUR T1 - Caveolin-1-mediated STAT3 activation determines electrotaxis of human lung cancer cells. AU - Li,Li, AU - Zhang, ... "Caveolin-1-mediated STAT3 Activation Determines Electrotaxis of Human Lung Cancer Cells." Oncotarget, vol. 8, no. 56, 2017, pp ...
In contrast, caveolin-marked lipid raft domains do not mediate entry of C. trachomatis serovars A, 36B, and C, nor of LGV ... Caveolin 1. Caveolins / metabolism*. Chlamydia / drug effects, metabolism*, pathogenicity*. Chlamydia Infections / drug therapy ... Finally, we show that entry of each of these chlamydial strains is independent of cellular expression of caveolin-1. Thus, ... 12799183 - Lipid rafts, caveolae, caveolin-1, and entry by chlamydiae into host cells.. 7115463 - The cell population of aortic ...
... caveolin-2, and caveolin-3. Alignment of the protein sequences encoded by these caveolin genes is shown in Fig. 1a ⇓ . Note ... and caveolin-3. a, conserved amino acid residues are boxed. A Pro-Leu substitution at amino acid residue 105 in human caveolin- ... Invasion Activating Caveolin-1 Mutation in Human Scirrhous Breast Cancers. Kazuhiko Hayashi, Satoru Matsuda, Kazuya Machida, ... Invasion Activating Caveolin-1 Mutation in Human Scirrhous Breast Cancers. Kazuhiko Hayashi, Satoru Matsuda, Kazuya Machida, ...
The phosphorylation of caveolin-2 on serines 23 and 36 modulates caveolin-1-dependent caveolae formation. Sowa, G., Pypaert, M ... Identification, sequence, and expression of caveolin-2 defines a caveolin gene family. Scherer, P.E., Okamoto, T., Chun, M., ... The caveolin proteins (caveolin-1, -2, and -3) serve as the structural components of caveolae, while also functioning as ... Brefeldin A treatment induced further accumulation of caveolin-2 along with caveolin-1 in LD [23]. ...
Oxytocin receptor elicits different EGFR/MAPK activation patterns depending on its localization in caveolin-1 enriched domains. ... Rimoldi V1, Reversi A, Taverna E, Rosa P, Francolini M, Cassoni P, Parenti M, Chini B. ... 1. CNR Institute of Neuroscience, Cellular and Molecular Pharmacology Section, Via Vanvitelli 32, 20129 Milan, Italy.. ... shown that oxytocin inhibits cell proliferation when the vast majority of oxytocin receptors are excluded from caveolin-1- ...
1998) Caveolin-mediated regulation of signaling along the p42/44 MAP kinase cascade in vivo: a role for the caveolin- ... 2011) Caveolin-1 is required for vascular endothelial insulin uptake. Am J Physiol Endocrinol Metab 300:E134-E144. doi:10.1152/ ... 1995) Insulin stimulates the tyrosine phosphorylation of caveolin. J Cell Biol 129:1523-1531. doi:10.1083/jcb.129.6.1523 pmid: ... 2014) Regulation of intracellular signaling and function by caveolin. FASEB J 28:3823-3831. doi:10.1096/fj.14-252320 pmid: ...
Trying to overexpress caveolin-1 in human cancer cells...are there readymade products out there which already has cav-1 ... where to get vector overexpressing caveolin-1? - posted in Molecular Cloning: ... Trying to overexpress caveolin-1 in human cancer cells...are there readymade products out there which already has cav-1 ... where to get vector overexpressing caveolin-1?. Started by phdgirl, May 21 2012 06:21 AM ...
The current results suggest interactions between c-Myc and caveolin-1 in the progression of human prostate carcinoma. ... The coexpression of c-Myc and caveolin-1 showed potential as a useful prognostic marker for human prostate carcinoma. ... Caveolin-1 is associated with prostate carcinoma progression and is a downstream target gene of c-Myc. The observation that ... Combined c-Myc and caveolin-1 expression in human prostate carcinoma predicts prostate carcinoma progression Cancer. 2005 Mar ...
Caveolin-1 ELISA Kits für viele Reaktivitäten. Huhn, Rind (Kuh), Hund und weitere. Caveolin-1 ELISA Kits vergleichen und ... Caveolin-1 (CAV1) Antigen-Profil Protein Überblick The scaffolding protein encoded by this gene is the main component of the ... Caveolin-1 ELISA Kit (Caveolin 1, Caveolae Protein, 22kDa) ELISA Kit CAV1 Reaktivität: Human pTyr14 Colorimetric Cell ELISA ... Caveolin-1 ELISA Kit (Caveolin 1, Caveolae Protein, 22kDa) ELISA Kit CAV1 Reaktivität: Maus Colorimetric Sandwich ELISA 0.15 ng ...
  • Caveolin-1 is a protein that in humans is encoded by the CAV1 gene. (
  • Caveolin-1 (Cav1) is a scaffold protein and pathogen receptor in the mucosa of the gastrointestinal tract. (
  • Infection of AGS/Cav1 cells triggered the recruitment of p120 RhoGTPase-activating protein/deleted in liver cancer-1 (p120RhoGAP/DLC1) to Cav1 and counteracted CagA-induced cytoskeletal rearrangements. (
  • Mechanistically, H. pylori activated sterol-responsive element-binding protein-1 (SREBP1) to repress transcription of the human Cav1 gene from sterol-responsive elements (SREs) in the proximal Cav1 promoter. (
  • In our study, we found that the tumor suppressor caveolin-1 (Cav1) is reduced upon infection with H. pylori , and CagA was sufficient but not necessary for this down-regulation. (
  • Loss of Cav1 was caused by H. pylori -dependent activation of sterol-responsive element-binding protein-1 (SREBP1), and this event abolished the interaction of Cav1 with p120 RhoGTPase-activating protein/deleted in liver cancer-1 (p120RhoGAP/DLC1), a second bona fide tumor suppressor in gastric tissue. (
  • Synthetic 16 amino acid peptide from N-terminus of human CAV1 / Caveolin. (
  • The expression of caveolin-1 (CAV1) in both tumor cell and cancer-associated fibroblasts (CAFs) has been found to correlate with tumor aggressiveness in different epithelial tumor entities, whereas less is known for caveolin-2 (CAV2). (
  • Caveolin-1 (CAV1) and caveolin-2 (CAV2) are integral membrane proteins found in the outer cell membrane called caveolae and in other intracellular membranes of the endoplasmic reticulum, Golgi apparatus, and transport vesicles [ 1 , 2 ]. (
  • Interplay Between Statins, Cav1 (Caveolin-1), and Aldosterone. (
  • Caveolin-1 or CAV1 is a scaffolding proteins within caveolar membranes. (
  • 32 Caveolin 1, Caveolae Protein, 22kDa (CAV1) ELISA Kits von 10 Herstellern verfügbar auf (
  • Rat brain lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAV1 (1:1000). (
  • The loss of Caveolin-1 (Cav1) in the tumor stromal compartment has emerged as a novel biomarker for predicting poor clinical outcome in all of the most common subtypes of breast cancer, however the mechanism of Cav1 loss is unknown. (
  • Caveolae and their structural protein caveolin 1 (CAV1) have roles in cellular lipid processing and systemic lipid metabolism. (
  • Here, we show that Rac1 binds the adapter protein caveolin-1 (Cav1) and that Rac1 activity promotes Cav1 accumulation at Rac1-positive peripheral adhesions. (
  • Mechanistically, the latter is mediated via FAK/Src/ERK/AKT pathways leading to the induction of the oncogene caveolin-1 (Cav1). (
  • Caveolin-1 (cav1) is reported to have both cell survival and pro-apoptotic characteristics. (
  • The protein levels of caveolin‑1 (Cav‑1) and copper-zinc superoxide dismutase (SOD)1 were determined by western blotting, and the interaction of Cav1 and HSC70 was investigated by co‑immunoprecipitation experiments. (
  • Caveolin-1 (Cav1) is an important component of caveolae and is known to function as a scaffolding protein in the regulation of several signaling pathways ( 7 - 9 ). (
  • Caveolin-1 (CAV1) is a multifunctional protein and a major component of caveolae. (
  • PPARγ upregulates and interacts with caveolin-1 (Cav1), a scaffold protein of Ras/mitogen-activated protein kinases (MAPKs). (
  • Cav1 cooperated with the endogenous Ras/MAPK inhibitor docking protein 1 (Dok1) to promote the ligand-dependent transcriptional activity of PPARγ and to inhibit cell proliferation. (
  • Frequency of common polymorphisms in Caveolin 1 (CAV1) gene in adults with high serum triglycerides from Colombian Caribbean Coast. (
  • Caveolin 1 gene (CAV1) has been associated with insulin resistance, metabolic syndrome and hypertension in humans. (
  • Caveolin-1 (Cav1) is a structural protein of caveolae linked to TGF-β receptors trafficking and signaling. (
  • Caveolin-1 (CAV1), Twist and cytokeratin (CK) in rinsed and intact myoma in normoxia and hypoxia [cobalt chloride (CoCl 2 ) or in hypoxia chamber with 1% O 2 )] (a-f) show similar patterns of expression in all settings: positive nuclear staining of the invading HSC-3 islands with Twist (most nuclei) and diffuse cytoplasmic staining with cytokeratin and, to a lesser extent, with CAV1 (arrows). (
  • Caveolin-1 knockout mice (Cav1(-/-)) were used to examine the effect of caveolin-1 absence on the NO function in the mouse small intestine (ileum and jejunum) compared to their genetic controls and BALB/c controls. (
  • To investigate the effects of caveolin-1 (CAV1) on the growth of hypopharyngeal squamous cellcarcinoma (HSCC) FaDu cells in vitro and in vivo. (
  • A CAV1-RNAi-lentivirus construct was transfectedinto FaDu cells and expression of caveolin-1 was tested by RT-PCR and western blotting analysis. (
  • Rabbit IgG polyclonal antibody for Caveolin-1(CAV1) detection. (
  • Caveolin-1 (Cav1) is an essential protein for its formation and structural stability. (
  • Src tyrosine kinases, Galpha subunits, and H-Ras share a common membrane-anchored scaffolding protein, caveolin. (
  • Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. (
  • Caveolin-1, a 21- to 24-kd integral membrane protein, is primarily implicated as a tumor suppressor gene. (
  • The involvement of these membrane domains in the entry of these organisms was indicated by the sensitivity of their entry to the raft-disrupting agents Nystatin and filipin, and by their intracellular association with caveolin-1, a 22-kDa protein associated with the formation of caveolae in rafts. (
  • We looked for mutations in the caveolin-1 gene, encoding a critical molecule for membrane signaling to cell growth, in 92 primary human breast cancers, and we report here the identification of a mutation in caveolin-1 at codon 132 (P132L) in 16% of cases. (
  • This site is evolutionarily conserved from worms to man, providing evidence that this region of the membrane-spanning domain is critical for Caveolin function. (
  • We have recently shown that oxytocin inhibits cell proliferation when the vast majority of oxytocin receptors are excluded from caveolin-1-enriched microdomains, and that, on the contrary, it has a mitogenic effect when the receptors are targeted to these plasma membrane domains. (
  • In this study, we examined the effects of perturbations in cholesterol/caveolin-1 (CAV-1)/caveolae homeostasis on the membrane properties and adhesive characteristics of MSCs. (
  • Findings from this study will contribute to the understanding of how cholesterol/CAV-1/caveolae regulates aspects of the cell membrane important to cell adhesion, substrate sensing, and microenvironment interaction. (
  • Cholesterol supplementation to MSCs increased membrane cholesterol, and resulted in decreased membrane fluidity and localization of elevated numbers of caveolae and CAV-1 to the cell membrane. (
  • Conversely, knockdown of CAV-1 expression or cholesterol depletion on MSCs caused a parallel decrease in caveolae content and an increase in membrane fluidity due to decreased delivery of cholesterol to the cell membrane. (
  • Our results demonstrate that perturbations in cholesterol/CAV-1 levels significantly affect the membrane properties of MSCs. (
  • These findings suggest that modification of membrane cholesterol and/or CAV-1 and caveolae may be used to manipulate the biological activities of MSCs. (
  • Caveolin-1 is present on both the apical and basolateral plasma membrane, whereas caveolin-2 is enriched on the basolateral surface where caveolae are present. (
  • This suggests that caveolin-1 and -2 heterooligomers are involved in caveolar biogenesis in the basolateral plasma membrane. (
  • In contrast, smaller caveolin-1/-2 heterooligomers are routed to the basolateral membrane where caveolin-2 is enriched and caveolar structures are found. (
  • Wild-type (WT) and Caveolin 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500. (
  • Mouse tissue extracts (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500. (
  • Immunoprecipitation of Caveolin 1 protein from A549 membrane extracts using 5 μg of Caveolin 1 antibody [N1N3] (GTX100205). (
  • Caveolin 1 antibody [N1N3] detects Caveolin 1 protein at membrane by immunofluorescent analysis. (
  • Caveolae, plasma membrane invaginations with constitutive caveolin proteins, harbour proteins involved in intracellular calcium ([Ca 2+ ] i ) regulation. (
  • Caveolae are flask-shaped plasma membrane invaginations expressing the scaffolding caveolin proteins-1 to -3 [ 1 , 2 ], as well as a variety of proteins important in signal transduction [ 3 ]. (
  • In the present study, we demonstrated that caveolin-1, a major membrane scaffolding protein, plays a critical role in experimental autoimmune encephalomyelitis (EAE), a laboratory murine model of multiple sclerosis (MS). Immunized WT mice have increases of caveolin-1 expressions in serum and CNS tissues associated with disease development. (
  • Mammalian sprouty-1 and -2 are membrane-anchored phosphoprotein inhibitors of growth factor signaling in endothelial cells. (
  • Recently, Sprouty, an inhibitor of Drosophila development-associated RTK signaling, has been discovered.They are phosphorylated on serine residues and, upon growth factor stimulation, a subset is recruited to the leading edge of the plasma membrane.The data indicate that mammalian Spry-1 and -2 are membrane-anchored proteins that negatively regulate angiogenesis-associated RTK signaling, possibly in a RTK-specific fashion. (
  • The data indicate that mammalian Spry-1 and -2 are membrane-anchored proteins that negatively regulate angiogenesis-associated RTK signaling, possibly in a RTK-specific fashion. (
  • Previous studies have described individual cellular elements with potential mechanosignaling properties, including ion channels, 6 integrins, 7 the glycocalyx, 8 cilia, 9 platelet endothelial cell adhesion molecule-1 (PECAM-1), 10 various receptors for humoral compounds, 11 the cytoskeleton, 12 and the plasma membrane, including lipid raft and caveolar subdomains. (
  • Although downregulation of caveolin-1 (Cav-1), which is a key constituent of membrane caveolae and a regulator of cellular processes, is associated with colorectal cancer (CRC), its involvement in the disease progression is largely unknown. (
  • Caveolin knockdown significantly decreased Gα s localization in detergent insoluble lipid raft/caveolae membrane fractions, suggesting that caveolin localizes a portion of Gα s to these membrane microdomains. (
  • Proteins involved in ion and water transport localize to caveolae, flask-shaped invaginations of the plasma membrane containing the coat protein, Cav-1. (
  • In this study we show that Dsg2 associates with caveolin-1 (Cav-1), the major protein of specialized membrane microdomains called caveolae, which functions in both membrane protein turnover and intracellular signaling. (
  • Glenney JR Jr, Soppet D. Sequence and expression of caveolin, a protein component of caveolae plasma membrane domains phosphorylated on tyrosine in Rous sarcoma virus-transformed fibroblasts. (
  • Instead, we show that hypoxia-induced translocation of caveolin-1 from cytoplasmic vesicles to the plasma membrane contributes to deficient trastuzumab internalization and T-DM1 chemosensitivity. (
  • Caveolin-1 (cav-1) is a transmembrane protein identified in plasma membrane caveolae, and a potential modulator of renal and vascular function and insulin sensitivity. (
  • Caveolin is a cholesterol-binding membrane protein involved in cellular cholesterol transport. (
  • Caveolin (Cav) is a special vesicle-like structure on the surface of the cell membrane and is an important structural-functional protein. (
  • We have addressed this question by examining the impact of membrane rafts and caveolin-1 on the differential signaling of mouse beta(3a)- and beta(3b)-AR isoforms that diverge at the distal C terminus. (
  • When cells expressing the beta(3a)-AR were treated with filipin III to disrupt membrane rafts or transfected with caveolin-1 siRNA, the cyclic AMP response to the beta(3)-AR agonist CL316243 became PTX-sensitive, suggesting G alpha(i/o) coupling. (
  • Caveolin-1(Cav-1) is an essential constituent protein of specialized membrane invaginations, referred to as caveolae. (
  • Other endothelial membrane proteins (PECAM-1, Tie2, sGC) are also lost indicating extensive endothelial membrane damage. (
  • Based on these results, Dr. Mathew's group hypothesizes that the endothelial damage is progressive and the loss of vWF indicates extensive damage/loss of endothelial cells, which may expose SMC to direct pressure/stress, leading to enhanced expression of caveolin-1 and possible translocation in SMC membrane. (
  • Caveolin-1 is a plasma membrane-associated protein that is responsible for caveolae formation. (
  • Background: Caveolin-1 (Cav-1), the major component of caveolae, is a 21-24 kDa integral membrane protein that interacts with a number of signaling molecules. (
  • Endothelial nitric oxide synthase (eNOS), one of three NO-producing enzymes, is located in a close conformation with the caveolin-1 (CAV-1 WT ) membrane protein which is inhibitory to NO production. (
  • Caveolin forms oligomers and associates with cholesterol and sphingolipids in certain areas of the cell membrane , leading to the formation of caveolae . (
  • The following antibody was used in this experiment: Caveolin 1 Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-32690, RRID AB_2550153. (
  • Flow Cytometry: Caveolin-1 Antibody (7C8) [Allophycocyanin] [NB100-615APC] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615APC (blue) and a matched isotype control (orange). (
  • This Caveolin 1 antibody is useful for Flow Cytometry, Western blot, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Immunohistochemistry-paraffin embedded sections. (
  • Western blot analysis of extracts from HuvEc cells treated with PMA 125ng/ml 30' and HeLa cells treated with LPS 100ng/ml 30', using Caveolin-1 (Phospho-Tyr14) Antibody. (
  • Western blot (WB) analysis of p-Caveolin-1 (pTyr14) antibody Cat. (
  • Immunofluorescence staining of methanol-fixed HeLa cells using Caveolin-1 pTyr14 antibody (#AP02388PU, Red). (
  • Western blot analysis of the extract from NIH/3T3 cells untreated or treated with H2O2 using Caveolin-1 antibody (AP02643PU, Lane 1 and 2) and Caveolin-1 pTyr14 antibody (#AP02388PU, Lane 3 and 4). (
  • Immunohistochemical microscopy analysis of paraffin-embedded lung carcinoma cell line xenograft tissue using Caveolin-1 antibody [N1N3] (GTX100205) (1:100). (
  • Caveolin 1 antibody detects Caveolin 1 protein by western blot analysis. (
  • Western blot analysis was performed using Caveolin 1 antibody [N1N3] (GTX100205). (
  • Green: Caveolin 1 protein stained by Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500. (
  • Various lysates were subjected to SDS PAGE followed by western blot with 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. (
  • Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 10x lens). (
  • Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 40x lens). (
  • Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017! (
  • Similar results were obtained by avoiding caveolin-1 expression with the interference RNA technique, and the administration of glucose in mice without caveolin allowed them to have an alternative energy source and were able to regenerate liver with more normality. (
  • Expression and characterization of recombinant caveolin. (
  • The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types. (
  • Cell-type and tissue-specific expression of caveolin-2. (
  • Long-time course of protease-activated receptor-1 expression after intracerebral hemorrhage in rats," Neuroscience Letters , vol. 459, no. 2, pp. 62-65, 2009. (
  • Re-expression of caveolin-1 is found in advanced human and mouse prostate adenocarcinomas. (
  • To explore its potential role in tumorigenesis and tumor progression of human lung cancers, we used the well-characterized cell line (CL) series of lung adenocarcinoma cells with increasing cellular invasiveness to show that expression of caveolin-1 mRNA and protein was up-regulated with enhanced invasion/metastatic capability of CL cells. (
  • Thus, a correlation exists for both constitutive and induced expression of caveolin-1 in CL cells. (
  • Expression of caveolin-1 protein in BEAS-2B cells, CL1-5, and CL1-5F4 cells, but not in CL1-0 and CL1-1 cells. (
  • Finally, we show that entry of each of these chlamydial strains is independent of cellular expression of caveolin-1. (
  • In cell lines expressing the same mutant of caveolin-1 , we observed that the mutant Caveolin-1 expression seemed to induce cellular transformation and activation of mitogen-activated protein kinase-signaling pathway and to promote invasion-ability as well as altered actin networks in the cells. (
  • Parental NIH3T3 cells were transfected, with the mutant or wild-type caveolin-1 genes introduced into pcDNA3 expression vector using a lipofection protocol. (
  • Here we show that the expression of caveolin-1 (Cav-1) is reduced in the brain in Type 2 diabetes mellitus. (
  • In this study, the authors evaluated the prognostic potential of combined c-Myc and caveolin-1 expression in human prostate carcinoma progression. (
  • Each cell group was analyzed for perturbation of cholesterol status and CAV-1 expression by performing Amplex Red cholesterol assay, filipin fluorescence staining, and real-time polymerase chain reaction (PCR). (
  • Cells with depleted CAV-1 expression showed decreased cell surface integrin expression and slower adhesion to different substrates. (
  • We have previously shown that cav-1 expression is increased in metastatic human prostate cancer and that cav-1 cellular protein expression is predictive of recurrence of the disease after radical prostatectomy. (
  • In contrast, co-expression of caveolin-1 scaffolding domain deletion mutant Cav-1Δ80 −100 did not affect hSlo current density (58.4±14.6 pA/pF, n=8, p=NS vs. control). (
  • Methods Mice or cells received the caveolin-1 scaffolding domain (CSD) peptide to reverse the pathological effects of reduced caveolin-1 expression. (
  • To validate the results in human disease and to identify caveolin-1-regulated molecular mechanisms, monocytes and neutrophils were isolated from patients with scleroderma and control subjects and caveolin-1, extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), p38, CXC chemokine receptor 4 (CXCR4) and matrix metalloproteinase 9 (MMP-9) expression/activation were evaluated. (
  • In bleomycin-treated mice, caveolin-1 expression was diminished in monocytes and CSD peptide inhibited leucocyte recruitment into the lungs. (
  • With this model, it was found that the expression levels of cav‑1 and Notch1 were significantly increased in brain tissues in the hypertension group compared with the sham‑operated group. (
  • Haines P, Samuel GH, Cohen H, Trojanowska M and Bujor AM: Caveolin-1 is a negative regulator of MMP-1 gene expression in human dermal fibroblasts via inhibition of Erk1/2/Ets1 signaling pathway. (
  • Small interference RNA (siRNA) or overexpression vectors were used to alter expression of caveolin-1 (Cav-1), Orai1 or STIM1. (
  • Cav-1 siRNA decreased caveolar and whole-cell Orai1 (but not STIM1) expression, and blunted SOCE, even in the presence of TNF-α. (
  • Cav-1 overexpression significantly increased Orai1 expression and SOCE, especially in the presence of TNF-α. (
  • In agreement with in vivo studies, in vitro silencing of caveolin-1 in endothelial cells prevented the up-regulation of ICAM-1 expression, leading to less trans-endothelial migration of encephalitogenic T H 1 and T H 17 cells. (
  • Because caveolin-1 has been shown previously to demonstrate antiapoptotic activities in prostate cancer cells, our results suggest that differences in caveolin-1 expression may in part underlie the apparent differences in the clinical virulence of this disease in AA versus white prostate cancer patients. (
  • In this study, we examined the expression of Caveolin-1 in human FVMs from patients with PVR. (
  • The alpha-smooth muscle actin (αSMA) expression and migration ability were increased in RPE cells with knockout or knockdown of Caveolin-1 , whereas zonula occludens-1 (ZO-1) immunohistochemistry showed reduced morphology and expression of ZO-1. (
  • Fresh tissues from patients with CRC and human CRC SW480 cells were used to evaluate Cav-1 and Ki-67 expression using immunohistochemistry and Western blotting. (
  • Distinct dedifferentiation processes affect caveolin-1 expression in hepatocytes. (
  • Caveolin-1 regulates the expression of tight junction proteins during. (
  • Gene expression was compared between Cav-1 null and control mice by microarray and qRT-PCR analyses. (
  • Expression of caveolin-1 in human adipose tissue is upregulated in obesity and obesity-associated type 2 diabetes mellitus and related to inflammation. (
  • The aim of the present study was to explore gene expression levels of CAV-1 in human adipose tissue in obesity and obesity-associated type 2 diabetes mellitus (T2DM) and to analyse its potential implication in the inflammatory state associated with obesity. (
  • Our findings show for the first time the upregulation of mRNA CAV-1 expression levels in VAT and SAT of obese NG and obese T2DM patients compared with lean controls, suggesting a role for CAV-1 in obesity and T2DM development. (
  • Members of the Ras/mitogen-activated protein kinase (MAPK) cascade, such as extracellular signal-regulated kinases 1/2 (ERK1/2), counteract this effect by inducing cyclin D1 expression and reducing PPARγ activity by phosphorylation on serine 84 (serine 82 in mouse) in its N-terminal activation function (AF1) ( 7 ). (
  • Calretinin and caveolin-1 expression by tumour in MM have recently been described to be associated with tumour histology, differentiation and consequently survival. (
  • No prognostic value was demonstrable for caveolin-1 expression and calretinin/caveolin-1 ratio. (
  • There was no relationship between calretinin and caveolin-1 expression. (
  • We searched the PubMed, ScienceDirect and Web of Science databases for published literature investigating associations between stromal caveolin-1 expression and survival outcome in breast cancer patients. (
  • Further investigation demonstrated that a lack of caveolin-1 expression in the breast cancer stroma is a hazard for overall survival (OS) (HR = 2.33, 95% CI: 1.57-3.46) and disease-free/progression-free survival (DFS/PFS) (HR = 3.05, 95% CI: 2.26-4.12) in breast cancer patients. (
  • Moreover, lack of caveolin-1 expression in the cancer-associated fibroblasts was a significant predictor of DFS/PFS (HR = 2.79, 95% CI: 1.75-4.46). (
  • Our results indicated that lack of expression of caveolin-1 in the breast cancer stroma is associated with a poor prognosis. (
  • Loss of stromal caveolin-1 expression predicts poor clinical outcome in triple negative and basal-like breast cancers. (
  • Simpkins SA, Hanby AM, Holliday DL, Speirs V. Clinical and functional significance of loss of caveolin-1 expression in breast cancer-associated fibroblasts. (
  • Expression and compartmentalization of caveolin in adipose cells: coordinate regulation with and structural segregation from GLUT4. (
  • Here, we examine the role of caveolin-1 (Cav-1) in regulating FASN expression during prostate cancer progression. (
  • Then, we assessed the expression of FASN in Cav-1 +/+ and Cav -1-/- prostate tumors by immuno-histochemistry and Western blot analysis. (
  • We conclude that Cav-1 expression is normally required for the upregulation of FASN during prostate cancer progression. (
  • These results also mechanistically explain why TRAMP/Cav-1 -/- mice are dramatically resistant to the development of prostate tumors and lung metastases, as they lack the expression of the FASN tumor promoter. (
  • HER2 and caveolin-1 expression was investigated in a well-annotated breast cancer cohort. (
  • Ectopic expression of cav-1 in both cell lines correlated with significantly decreased iNOS activity and protein levels. (
  • Increased caveolin-1 expression in Alzheimer's disease brain. (
  • Objective: Our study aimed to study the role of Cav-1 in gastric cancer progression and investigate the relationship between Cav-1/E-cadherin expression and the clinical status of gastric cancer. (
  • Method: Immunohistochemistry was applied to detect the expression of Cav-1 and E-cadherin in gastric cancer in a tissue microarray. (
  • Real-time PCR was used to further detect the mRNA expression of Cav-1 and E-cadherin in tumor-derived and peritumoral tissues and in different gastric cancer cell lines. (
  • The expression of E-cadherin was analyzed by Western Blot and the cell migration ability was examined by Transwell migration assays after downregulation of Cav-1 using siRNA. (
  • Moreover, there was a positive correlation between Cav-1 expression and E-cadherin expression in gastric cancer tissues(r = 0.42, P (
  • Knockdown of Cav-1 resulted in decreased expression of E-cadherin, cell morphology changes and elevated migration ability of gastric cancer cells. (
  • The current research aims to investigate caveolin-1 expression in prostate cancer tissue and its relationship with pathological grade, clinical pathologic staging, and preoperative prostate-specific antigen (PSA) levels. (
  • The expression of caveolin-1 is not associated with preoperative serum PSA levels. (
  • CK and alpha-smooth muscle actin (αSMA) in rinsed and intact myoma in normoxia and hypoxia [cobalt chloride (CoCl 2 ) or 1%O 2 )] (g-l) show similar patterns of expression in all settings: positive nuclear staining of the invading HSC-3 islands with Twist (most nuclei) and diffuse cytoplasmic staining with CK. (
  • In addition to transcriptional regulation of caveolin-1, the influence of caveolin-1 expression on cellular phenotypes like signal transduction and polyamine uptake were assessed. (
  • The present studies demonstrate that caveolin-1 expression affects basal levels of AKT and ERK signaling, with an increased signaling associated with caveolin-1 expression in these colon tumor-derived cells. (
  • In addition, caveolin-1 expression positively affects signaling in response to an inflammatory stimulus like TPA. (
  • Interestingly, caveolin-1 expression leads to a decrease in the uptake of pro-tumorigenic molecules like polyamines, in the colon cell lines tested. (
  • Taken together, the data from this study suggests that caveolin-1 is transcriptionally regulated by the APC and the K-RAS gene at different stages of colorectal tumorigenesis and this in turn, leads to different phenotypes influenced by caveolin-1 expression. (
  • Rajamma Mathew, MD and her research group have been studying the role of caveolin-1 expression during the progression of pulmonary hypertension. (
  • At 4 wks post-MCT, 29% of small arteries exhibit loss of von Willebrand Factor (vWF), and 70% of these arteries exhibited enhanced expression of caveolin-1 in smooth muscle cells(SMC). (
  • Lung biopsy revealed loss of endothelial caveolin-1 and vWF, associated with enhanced caveolin-1 expression in SMC. (
  • Induction of apoptosis was measured by flow cytometry, and the expression of caveolin-1 was examined by immunofluorescence microscopy, quantitative real-time reverse transcription polymerase chain reaction (RT-PCR), and immunoblotting. (
  • The expression of caveolin-1 was decreased, and the rate of apoptosis was increased in LECs treated with increased glucose concentration and treatment duration. (
  • We observed that in HG-treated LECs, caveolin-1 expression decreased and apoptosis increased and that simvastatin or EGF promoted the proliferation of HG-treated LECs. (
  • In the present study, we showed that high glucose (HG) concentrations in culture decreased the expression of caveolin-1 and caused LEC apoptosis while EGF, which usually increases in aqueous humor after cataract surgery, and simvastatin, an 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor commonly used in diabetic patients, enhanced the expression of caveolin-1 and reduced apoptosis in HG-treated LECs. (
  • Reductions in pulmonary Cav-1 expression have been shown in several animal models of PH and in patients with severe PH. (
  • Whether in vivo modulation of Cav-1 expression could affect the development of PH and RVH remains unknown. (
  • Mechanistically, the development of PH was associated with reduced expression of pulmonary Cav-1 and Cav-2, hyperactivation of the STAT3 signaling cascade, and upregulation of cyclin D1 and D3 protein levels, all of which were prevented by administration of AP-Cav. (
  • NO production was enhanced by lentiviral vector co-delivery of eNOS and CAV-1 F92A to eASCs, and osteogenesis and Wnt signaling was assessed by gene expression analysis and activity of a novel Runx2-GFP reporter. (
  • Co-expression of eNOS and CAV-1 WT transgenes resulted in lower NO production. (
  • Lentiviral vector expression of canonical Wnt3a and non-canonical Wnt5a in eASCs was associated with induced and suppressed osteogenic differentiation, respectively, whilst treatment of eNOS-osteogenic cells with the Wnt inhibitor Dkk-1 significantly reduced expressions of Runx2 and ALP. (
  • Presently, caveolin-1 expression is associated with more severe renal disease in human and previous murine studies. (
  • Using 2 time-points of the unilateral ureteric obstruction model, wild-type and caveolin-1 knockout mouse kidneys were analysed for caveolin-1 expression and markers of fibrosis using histology, Gomori staining, real-time quantified polymerase chain reaction, Western blotting and confocal microscopy. (
  • There was increased caveolin-1 expression the longer the unilateral obstruction occurred as well as in the contralateral compensating non-obstructed kidney. (
  • Caveolin-1 expression manipulation timing remains to be elucidated in reducing renal fibrosis. (
  • The expression of caveolin-3 is restricted to striated and smooth muscle . (
  • Caveolin interacts with and regulates heterotrimeric G-proteins. (
  • Caveolin-1 regulates nitric oxide-mediated matrix metalloproteinases activity and blood-brain barrier permeability in focal cerebral ischemia and reperfusion injury," Journal of Neurochemistry , vol. 120, no. 1, pp. 147-156, 2012. (
  • Calycosin-7-O- β -d-glucoside regulates nitric oxide /caveolin-1/matrix metalloproteinases pathway and protects blood-brain barrier integrity in experimental cerebral ischemia-reperfusion injury," Journal of Ethnopharmacology , vol. 155, no. 1, pp. 692-701, 2014. (
  • Caveolin-1 selectively regulates microRNA sorting into microvesicles after noxious stimuli. (
  • Caveolin-1 Regulates Atherogenesis by Attenuating Low-Density Lipoprotein Transcytosis and Vascular Inflammation Independently of Endothelial Nitric Oxide Synthase Activation. (
  • Together, these results suggest that Cav-1 regulates APP metabolism, and that depletion of Cav-1 in T2DM promotes the amyloidogenic processing of APP and hyperphosphorylation of tau. (
  • This is specific for caveolin alpha and beta proteins. (
  • A Pathway Studio v.11 analysis of the soluble and cell-associated proteins together with protein kinase C alpha (PRKCA) suggests that caveolin-1 is involved, when plasminogen enriched in the extracellular space is not activated and the vascular cellular adhesion molecule (VCAM-1) mediated cell-cell adhesion is simultaneously strengthened and activated PRKCA is recruited in caveolae, while the thyroid cancer cells do not form spheroids. (
  • SOCE involves proteins Orai1 and stromal interaction molecule (STIM)1. (
  • We and others have demonstrated that human airway smooth muscle (ASM) cells express caveolin-1 (Cav-1), and that ASM caveolae contain a number of proteins involved in regulation of intracellular Ca 2+ ([Ca 2+ ] i ) [ 6 - 8 ]. (
  • However, more recent evidence highlights the role of two proteins: Orai1 and stromal interaction molecule (STIM)1 [ 13 , 14 , 18 - 20 ]. (
  • 144 caveolin proteins are present in a single caveola ( Parton and Simons, 2007 ). (
  • Precipitated proteins and nonprecipitated proteins (Unbound) were resolved by SDS-PAGE, and caveolin-1, mSpry-1, and annexin II were detected by immunoblotting as indicated. (
  • Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. (
  • however, it is not known whether caveolin-1 is associated with the beta(3)-AR or solely with downstream signaling proteins. (
  • Our studies indicate that interaction of the beta(3a)-AR with caveolin inhibits coupling to G alpha(i/o) proteins and suggest that signaling is modulated by a raft-enriched complex containing the beta(3a)-AR, caveolin-1, G alpha(s), and adenylyl cyclase. (
  • Binding of caveolin-1 to these signal proteins inhibits their activity. (
  • Mammals have three caveolin proteins:caveolin-1 (Cav-1, or VIP21), caveolin-2 and caveolin-3 (or M-caveolin). (
  • Caveolae are enriched with cholesterol and Cav-1 is one of the few proteins that binds cholesterol tightly and specifically. (
  • Evaluating caveolin interactions: do proteins interact with the caveolin scaffolding domain through a widespread aromatic residue-rich motif? (
  • Depletion of caveolin-1 blocked the decline in p190RhoGAP tyrosine phosphorylation observed at later points by sustaining Src family kinase activity. (
  • Acute shear stress applied to cultured endothelial cells resulted in integrin-dependent phosphorylation of caveolin-1 (pY14) via a Src family kinase (SFK). (
  • 17 The phosphorylation of caveolin-1 served to recruit C-terminal Src-like kinase (Csk) to the integrin/caveolin-1 complex, resulting in additional regulation of SFK activity and induction of myosin light chain (MLC) phosphorylation. (
  • We find that phosphorylation-deficient caveolin-1 inhibits trastuzumab internalization and T-DM1 cytotoxicity, suggesting a specific role for caveolin-1 phosphorylation in HER2 trafficking. (
  • Here the role of Src-dependent Cav-1 phosphorylation in eNOS negative feedback regulation is investigated. (
  • Finally, Src FRET biosensor, eNOS small interfering RNA, and NO donor studies demonstrate NO-induced Src activation and Cav-1 phosphorylation at Tyr-14, resulting in increased eNOS/Cav-1 interaction and inhibition of eNOS activity. (
  • Taken together, these data suggest that activation of eNOS promotes Src-dependent Cav-1-Tyr-14 phosphorylation and eNOS/Cav-1 binding, that is, eNOS feedback inhibition. (
  • Caveolins 1 and 2 co-localize and form a stable hetero-oligomeric complex in vivo," The Journal of Biological Chemistry , vol. 272, no. 46, pp. 29337-29346, 1997. (
  • Genetically engineered mice that lack caveolin-1 and caveolin-2 are viable and fertile, showing that neither the caveolins nor the caveolae are essential in embryonic development or reproduction of these animals. (
  • Caveolin-1 (cav-1), the major protein component of caveolae, plays an important role in multiple signaling pathways, molecular transport, and cellular proliferation and differentiation. (
  • IDIBAPS researchers studied the role of caveolin-1 in this process, comparing the regenerative capacity of normal mice and modified mice, which do not express the caveolin-1 gene. (
  • Reintroducing the caveolin-1 gene into the less invasive, caveolin-1-negative CL cells enhanced their invasive capability at least by twofold, as revealed by an in vitro chamber invasion assay. (
  • Caveolin-1 is associated with prostate carcinoma progression and is a downstream target gene of c-Myc. (
  • Mice with homozygous deletion of the Cav-1 gene display cardiac hypertrophy and pulmonary abnormalities characterized by thickened alveolar septa, hypercellularity and PH. (
  • Methylation of a CpG island in the 5' promoter region of the caveolin-1 gene in human breast cancer cell lines. (
  • We have shown that cav-1 gene variants are associated with insulin resistance (IR) in hypertensive humans, and that cav-1 deficiency in mice leads to increased blood pressure (BP) and IR. (
  • In contrast, amlodipine treatment showed small changes in BP and IR but had adverse effects on vascular reactivity in cav-1 KO mice, raising the concern that it could have similar adverse effects on vascular function in hypertensive individuals that carry the cav-1 minor allele gene variants. (
  • Caveolin-1 is transcriptionally regulated by the APC tumor suppressor gene, via induction of its inducer, FOXO1 and the suppression of its transcriptional repressor, C-MYC. (
  • Caveolin-1 gene knockout impairs nitrergic function in mouse small intestine. (
  • The data from this study suggest that caveolin-1 gene knockout causes alterations in the smooth muscles and the ICC, leading to an impaired NO function in the mouse small intestine that could possibly be compensated by apamin-sensitive inhibitory mediators. (
  • Mice with homozygous deletion of the Cav-1 gene develop PH and right ventricular hypertrophy (RVH). (
  • Canonical Wnt signaling pathway-associated Wnt3a and Wnt8a gene expressions were increased in eNOS-CAV-1 F92A cells undergoing osteogenesis whilst non-canonical Wnt5a was decreased and similar results were seen with NONOate treatment. (
  • Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. (
  • Furthermore, Cav-1 is enriched in endothelial cells and plays a major role in regulation of trafficking via the blood-brain barrier. (
  • Here, we report the functional characterization of two of them, Spry-1 and -2, in endothelial cells. (
  • In contrast, although epidermal growth factor-induced proliferation of endothelial cells was also inhibited by Spry-1 and -2, activation of p42/44 MAP kinase was not affected. (
  • Similarly, stress fibers failed to form in endothelial cells exposed to enhanced blood flow in caveolin-1 knockout mice. (
  • 1 Several, now classic, studies 2 - 5 illustrate that in vitro and in vivo, actin stress fibers orient parallel to the direction of flow and are prominent in endothelial cells subjected to high shear velocities. (
  • Using fluorescence resonance energy transfer (FRET) and coimmunoprecipitation analyses, we observed increased interaction between eNOS and Cav-1 following stimulation of endothelial cells with thrombin, vascular endothelial growth factor, and Ca 2+ ionophore A23187, which is corroborated in isolated perfused mouse lung. (
  • Cav-1 and the caveolae structure are believed to be involved in multiple cellular processes that include signal transduction, lipid regulation, endocytosis, transcytosis, and mechanoprotection. (
  • We hypothesized that caveolin-1, a protein that controls the regulation of endothelial vesicular trafficking and signal transduction, is associated with negative remodeling in MMD. (
  • The data of the present study demonstrated that the cav‑1/Notch signaling plays an important role in the regulation of Ang‑II‑induced hypertension and vascular remodeling. (
  • The manipulation of β1 integrin and caveolin-1 also altered shear regulation of RhoA activity. (
  • Together, our data for the first time identify hypoxic regulation of caveolin-1 as a resistance mechanism to T-DM1 with potential implications for individualized treatment of breast cancer. (
  • Caveolin-1 is involved mainly in the interaction of signaling molecules and the regulation of signaling channels, as well as the regulation of cell proliferation, differentiation, migration, apoptosis, and angiogenesis signaling pathways. (
  • In the present study, the transcriptional regulation of a novel target of these two genes, caveolin-1, was studied. (
  • The involvement of caveolin-1 in the regulation of apoptosis has been previously demonstrated in epithelial cells. (
  • By acting as a scaffolding protein, Cav-1 plays crucial roles in the regulation of various physiologic and patho-physiologic processes including oncogenic transformation and tumorigenesis, and tumor invasion and metastasis. (
  • The up-regulation of Cav-1 following DNA damage occurred only in cells expressing endogenous Cav-1, and was associated with the activation of DNA damage response pathways. (
  • The observation that caveolin-1 can suppress c-Myc-induced apoptosis suggested the potential for cooperation between c-Myc and caveolin-1 in malignant progression. (
  • In cultured HUVECs, knockdown of cav‑1 regulated Ang‑II‑induced HUVEC viability and apoptosis, and modulated hypertensive vascular remodeling, which was mediated by the Notch pathway. (
  • To address this issue, we used specific quantitative immunostaining protocols to determine whether a panel of biomarkers related to apoptosis including caveolin-1, bcl-2, p53, and c- myc were differentially expressed in AA versus white prostate cancer patients with similar clinical and pathological characteristics. (
  • In this study, we investigated the relationship between caveolin-1 and apoptosis of LECs under high glucose (HG) concentrations to explore a mechanism in the formation of PCO in diabetic patients. (
  • When simvastatin or EGF was added to HG-treated LECs, caveolin-1 levels increased and the apoptosis rate of LECs decreased. (
  • Genetically modified mice, not expressing caveolin-1, were incapable of forming the lipidic bodies necessary in order to provide energy for the regeneration. (
  • Previous work showed that adherens junctions become permeable in mice lacking caveolin-1, suggesting that the protein helps seal the junctions. (
  • Depletion of Cav-1 is recapitulated in the brains of db/db ( Lepr db ) diabetic mice and corresponds with recognition memory deficits as well as the upregulation of amyloid precursor protein (APP), BACE-1, a trending increase in β-amyloid Aβ 42/40 ratio and hyperphosphorylated tau (p-tau) species. (
  • Importantly, we show that restoration of Cav-1 levels in the brains of male db/db mice using adenovirus overexpressing Cav-1 (AAV-Cav-1) rescues learning and memory deficits and reduces pathology (i.e. (
  • Objectives Reduced caveolin-1 levels in lung fibroblasts from patients with scleroderma and the lungs of bleomycin-treated mice promote collagen overexpression and lung fibrosis. (
  • This study was undertaken to determine whether caveolin-1 is deficient in leucocytes from bleomycin-treated mice and patients with scleroderma and to examine the consequences of this deficiency and its reversal. (
  • In bleomycin-treated mice, the levels of caveolin-1 in leucocytes and the effect of CSD peptide on leucocyte accumulation in lung tissue were examined. (
  • Whether administration of such a Cav-1 peptide could rescue the cardio-pulmonary defects observed in Cav-1 KO mice remains unknown. (
  • Three week-old wild-type and Cav-1 KO mice were randomly assigned to receive a daily intraperitoneal injection of either saline, penetratin alone (AP, 2.5 mg/kg/d) or a peptide consisting of the Cav-1 scaffolding domain coupled to penetratin (AP-Cav, 2.5 mg/kg/d) for five weeks. (
  • Cav-1 KO mice receiving either saline or AP alone developed PH with respective RV systolic pressures (RVSP) of 35.6 ± 2.0 mmHg and 34.8 ± 1.7 mmHg. (
  • Daily administration of AP-Cav to Cav-1 KO mice significantly reduced the RVSP to 28.1 ± 1.5 mmHg. (
  • Cav-1 KO mice receiving saline or AP alone also displayed both left and right ventricular hypertrophy, which were prevented by daily delivery of AP-Cav. (
  • Lungs of Cav-1 KO mice receiving saline or AP alone further showed pulmonary artery medial hypertrophy, thickened alveolar septa and hypercellularity, which were all significantly improved by daily administration of AP-Cav. (
  • Administration of a cell-permeable Cav-1-mimetic peptide (AP-Cav) to Cav-1 KO mice reduces the development of pulmonary artery medial hypertrophy, PH and cardiac hypertrophy. (
  • We showed that angiotensin II (AngII)-induced pathologies in the heart and kidney of young, 3-month-old mice are suppressed by the caveolin-1 scaffolding domain (CSD) peptide. (
  • Furthermore, forskolin- and aluminum tetrafluoride-stimulated adenylyl cyclase activity was significantly increased by caveolin knockdown in cells or in brain membranes obtained from caveolin-1 knockout mice, indicating that caveolin attenuates signaling at the level of Gα s /adenylyl cyclase and distal to GPCRs. (
  • Mice null for Cav-1 have reduced retinal function as measured by electroretinography that is not detectable in recordings from isolated rods indicating that photoreceptors are healthy but that the photoreceptor microenvironment is pathological. (
  • Here, we report on an abnormality in the interaction between the retina and the RPE that may result from outer retinal edema in Cav-1 null mice. (
  • Eyecups from Cav-1 null and control mice were prepared and incubated in either Hank's balanced salt solution (HBSS) or HBSS containing 600 mM sucrose prior to peeling neural retinas from eyecups. (
  • Microarray, qRT-PCR, and immunoblot analyses revealed upregulation of regulators of Na/K-ATPase activity, FXYD2 and FXYD3 in Cav-1 null mice. (
  • Similar results have been observed following pharmacological inhibition of Na/K-ATPase activity by ouabain suggesting that the activity of the Na/K-ATPase is altered in retina and/or RPE from Cav-1 null mice. (
  • For this purpose, we crossed Cav-1 -/- null mice with TRAMP mice to generate TRAMP/Cav-1 +/+ and TRAMP/Cav-1 -/- mice. (
  • Thus, TRAMP/Cav-1 -/- mice will provide a novel model system to elucidate the role of FASN in prostate tumor progression. (
  • To test whether these changes in the cav-1 KO involve renal or vascular abnormalities, we treated WT and cav-1 KO mice for 14 days with placebo, the renin inhibitor aliskiren (50 mg/kg/day), or the Ca2+ channel blocker amlodipine (6 mg/kg/day) and assessed BP, fasting blood glucose, HOMA-IR, plasma aldosterone (ALDO), and vascular function. (
  • In cav-1 KO mice aliskiren significantly reduced BP to 113±4 mm Hg, ALDO levels by 40%, and HOMA-IR to 0.9±0.1, but had no significant effects on blood glucose, Phe-induced vasoconstriction, ACh- or exogenous NO-induced vasorelaxation. (
  • Thus aliskiren treatment in cav-1 KO mice reduced BP possibly through suppression of RAAS and improved insulin sensitivity, but did not affect vascular function. (
  • Curcumin Decreases Hyperphosphorylation of Tau by Down-Regulating Caveolin-1/GSK-3β in N2a/APP695swe Cells and APP/PS1 Double Transgenic Alzheimer's Disease Mice. (
  • The endogenous beta(3a)-AR displayed G alpha(i/o) coupling in brown adipocytes from caveolin-1 knock-out mice or in wild type adipocytes treated with filipin III. (
  • Cav-1 is the principal structural protein of caveolae and functions in signal transduction and lipid transport. (
  • Caveolin-1 (CAV-1) plays important roles in many aspects of cellular biology, including vesicular transport, cholesterol homeostasis and signal transduction. (
  • Summarising, this work makes two important contributions: On the one hand, it reveals the main vital function of lipid bodies and caveolin. (
  • In contrast, caveolin-marked lipid raft domains do not mediate entry of C. trachomatis serovars A, 36B, and C, nor of LGV serovar L2 and MoPn. (
  • Caveolin-1 (Cav-1) is a 22 kDa coat protein of caveolae, a subset of lipid rafts. (
  • C) The majority of mSpry-1 and caveolin-1 are not in detergent-resistant lipid rafts. (
  • Using a simplified model system in C6 glioma cells, this study disrupts rafts/caveolae using both pharmacological and genetic approaches to test whether caveolin-1 and lipid microdomains regulate G s trafficking and signaling. (
  • Lipid rafts/caveolae were disrupted in C6 cells by either short-term cholesterol chelation using methyl-β-cyclodextrin or by stable knockdown of caveolin-1 and -2 by RNA interference. (
  • Taken together, these results demonstrate that caveolin-1 and lipid microdomains exert a major effect on Gα s trafficking and signaling. (
  • Chand S, Hazeldine J, Smith SW, Borrows R (2018) Genetic Deletion of the Lipid Raft Protein Caveolin-1 Leads to Worsening Renal Fibrosis. (
  • Caveolin-1 is the essential structural protein for lipid rafts called caveolae that are ubiquitously distributed among fibroblasts, endothelial and epithelial cells. (
  • Primary Antibodies are guaranteed for 1 year from date of receipt. (
  • Immunostaining with c-Myc and caveolin-1-specific antibodies was performed on paraffin sections from 104 radical prostatectomy specimens from men with lymph node negative prostate carcinoma. (
  • Using rabbit polyclonal antibodies with specificity for cav-1, we developed a direct sandwich immunoassay for the determination of cav-1 in serum. (
  • Using HEK293 cells stably expressing hSlo (which encodes human BK channels) with and without transient transfection with caveolin-1, We found that hSlo channels were enriched in the low buoyant density fraction when co-expressed with caveolin-1 and the channels were co-immunoprecipitated by anti-caveolin-1 antibodies. (
  • Anti-caveolin-1 antibodies inhibit the apical delivery of influenza virus hemagglutinin without affecting basolateral transport of vesicular stomatitis virus G protein. (
  • Apical but not basolateral exocytosis can be inhibited with caveolin-1 antibodies, suggesting that two different caveolin complexes function in TGN to surface transport. (
  • Aliquots of the cell lysates were subjected to immunoprecipitations with antibodies specific for mSpry-1 (top) or unrelated rabbit immunoglobulins, as negative control (bottom), in the respective buffers. (
  • Loss of caveolin-1 activated the enzyme eNOS, which spawns nitric oxide (NO) that reacts to form peroxynitrite. (
  • In this study, we genetically modified equine adipose-derived stem cells (eASCs) with eNOS, CAV-1 WT , and a CAV-1 F92A (CAV-1 WT mutant) and assessed NO-mediated osteogenic differentiation and the relationship with the Wnt signaling pathway. (
  • however, the mechanism of Cav-1-mediated inhibition of activated eNOS is unclear. (
  • The eNOS/Cav-1 interaction is blocked by eNOS inhibitor L-N G -nitroarginine methyl ester (hydrochloride) and Src kinase inhibitor 4-amino-5-(4-chlorophenyl)- 7-(t-butyl) pyrazolo [3, 4-d] pyrimidine. (
  • We also observe increased binding of phosphomimicking Y14D-Cav-1 mutant transduced in human embryonic kidney cells overexpressing eNOS and reduced Ca 2+ -induced NO production compared to cells expressing the phosphodefective Y14F-Cav-1 mutant. (
  • Various classes of signalling molecules, including G-protein subunits, receptor and non-receptor tyrosine kinases, endothelial nitric oxide synthase (eNOS), and small GTPases, bind Cav-1 through its 'caveolin-scaffolding domain' [ PMID: 23028656 ]. (
  • Single channel recordings of hSlo channels in inside-out patches showed that exposure to cavtratin, a peptide containing the caveolin-1 scaffolding domain, dose-dependently inhibited hSlo opening probability (Po) with an IC 50 of 91.1±6.9 nM (n=5). (
  • The epitope-tagged and overexpressed caveolin-2 colocalized with caveolin-1 in fibroblasts, as judged by immunofluorescence microscopy. (
  • Electroacupuncture Exerts Neuroprotection through Caveolin-1 Mediated Molecular Pathway in Intracerebral Hemorrhage of Rats. (
  • Here, we aim to demonstrate that electroacupuncture on Baihui (GV20) exerts neuroprotection for acute ICH possibly via the caveolin-1/matrix metalloproteinase/blood-brain barrier permeability pathway. (
  • Taken together, these results suggest that endogenous EFs in the tumor micro-environment might play an important role in lung cancer metastasis by guiding cell migration through a Cav-1/STAT3-mediated signaling pathway. (
  • Key components of this pathway were recruited to caveolar microdomains and dependent on the presence of the caveolin-1 protein. (
  • An activated K-RAS oncogene induces caveolin-1 transcription via activation of the P-I3 Kinase pathway. (
  • Caveolin-1 acts as an intracellular signalling pathway chaperone in fibrotic disease. (
  • Replication Study: Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis. (
  • Up-regulated caveolin-1 accentuates the metastasis capability of lung adenocarcinoma by inducing filopodia formation. (
  • Caveolin-1 immunoreactivity was either totally absent or just barely detectable in a few lung adenocarcinoma cells from cases diagnosed as lung adenocarcinoma without regional lymph node metastasis. (
  • The MTS and Transwell assays were performed to determine the effects of Cav-1 overexpression via pcDNA3.1/Cav-1 plasmid on cell proliferation and metastasis. (
  • In these experiments, which are a subset of all the experiments reported in the original publication, Goetz and colleagues show in a subcutaneous xenograft model that stromal caveolin-1 remodels the intratumoral microenvironment, which is correlated with increased metastasis formation. (
  • however, this trafficking was blocked by methyl-β-cyclodextrin or by caveolin knockdown. (
  • Methyl-β-cyclodextrin or caveolin knockdown significantly increased isoproterenol or thyrotropin-stimulated cAMP accumulation. (
  • Knockdown of Cav-1 may promote EMT of gastric cancer by targeting E-cadherin. (
  • b Growth curves of the EPCs after knockdown of caveolin-1. (
  • Caveolin-1 interacts with androgen receptor. (
  • Santibanez JF, Blanco FJ, Garrido-Martin EM, Sanz-Rodriguez F, del Pozo MA and Bernabeu C: Caveolin-1 interacts and cooperates with the transforming growth factor-beta type I receptor ALK1 in endothelial caveolae. (
  • Caveolin interacts with specific lipids, such as glycosphingolipids or cholesterols, thereby leading to the formation of caveolae. (
  • Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1. (
  • There are two isoforms of caveolin-1: caveolin-1α and caveolin-1β, the latter lacking a part of the N-terminus. (
  • Immunohistochemical examination of caveolin-1 was performed in 95 specimens obtained retrospectively from patients who had lung adenocarcinoma either with (35 patients) or without (60 patients) ipsilateral hilar/peribronchial tumor-metastasized lymph nodes. (
  • Caveolin-1 was detected by streptavidin-perosidase (SP) immunohistochemical staining in pathological tissue slices. (
  • Such a broad biological role of Cav-1/caveolae is bound to have functional cross relationships with several molecular pathways including HIV replication and viral-induced pathogenesis. (
  • dissected the molecular chain of events that connects caveolin-1 to adherens junction integrity. (
  • Molecular cloning had identified three distinct caveolin genes (1 , 2 , 3 , 4) caveolin-1 , caveolin-2 , and caveolin-3 . (
  • These results suggest that the caveolin-1 scaffolding domain, and particularly F92, is a critical molecular site for inhibition of hSlo activities. (
  • 1 Instituto de Investigação e Inovação em Saúde and Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, Rua Alfredo Allen, 208 4200-135 Porto, Portugal. (
  • In addition, our results provide the first molecular genetic evidence that Cav-1 functions upstream of FASN during prostate cancer progression. (
  • However, research to explore the underlying molecular network between Cav-1 and stress signals is warranted. (
  • The molecular excess weight of cav-1 protein is normally 21 kDa. (
  • In this study, we tested the hypotheses that BK channels are inhibited by physical interaction with the scaffolding domain of caveolin-1, which alters the properties of the channel. (
  • Interaction with caveolin-1 reduces the sensitivity of hSlo to Ca 2+ and voltage activation. (
  • Mandarin fish caveolin 1 interaction with major capsid protein of infectious spleen and kidney necrosis virus and its role in early stages of infection. (
  • The beta(3a)-AR C terminus, S (P-384) under bar PLNR (P-389) under bar DG (Y-392) under bar EGARP (P-398) under bar PT, resembles a caveolin interaction motif. (
  • Modification of this interaction through mutation of the caveolin scaffold domain can increase NO release. (
  • Caveolin-1 (Cav-1) is both a tumor suppressor and an oncoprotein. (
  • Biochemical and immunofluorescence analysis of endogenous and overexpressed Spry-1 and -2 reveal that both Spry-1 and -2 are anchored to membranes by palmitoylation and associate with caveolin-1 in perinuclear and vesicular structures. (
  • Caveolin-1 depletion mimicked the hypoxic situation, indicating that vesicular caveolin-1 is indispensable for trastuzumab uptake and T-DM1 cytotoxicity. (
  • Conversely, dysregulation of caveolin-1 has been associated with several human diseases including multiple myeloma, an incurable malignancy characterized by excess monoclonal plasma cells within the bone marrow. (
  • Recombinant fragment corresponding to Human Caveolin-1 aa 1-104. (
  • Caveolin 1 (Cav-1) is a major component of the caveolae structure and is expressed in a variety of cell types including macrophages, which are susceptible to human immunodeficiency virus (HIV) infection. (
  • These results provide, for the first time, genetic evidence that a functioning Caveolin-1 mutation may have a role in the malignant progression of human breast cancer. (
  • Alignment of the protein sequences encoded by the human caveolin-1 , caveolin-2 , and caveolin-3 . (
  • Trying to overexpress caveolin-1 in human cancer cells. (
  • The coexpression of c-Myc and caveolin-1 showed potential as a useful prognostic marker for human prostate carcinoma. (
  • The current results suggest interactions between c-Myc and caveolin-1 in the progression of human prostate carcinoma. (
  • A recombinant human cav-1 fusion protein was overexpressed and purified from 293 PE cells and used as a calibrator. (
  • Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Caveolin 1. (
  • Cell migration assays were performed to evaluate the involvement of Caveolin-1 in EMT using primary human and mouse RPE cells. (
  • Caveolin-1 was expressed in human FVMs and upregulated in the mouse eyes with PVR. (
  • Sequence and detailed organization of the human caveolin-1 and -2 genes located near the D7S522 locus (7q31.1). (
  • Glenney JR, Jr. The sequence of human caveolin reveals identity with VIP21, a component of transport vesicles. (
  • To investigate whether caveolin-1 (cav-1) may modulate inducible nitric oxide synthase (iNOS) function in intact cells, the human intestinal carcinoma cell lines HT29 and DLD1 that have low endogenous cav-1 levels were transfected with cav-1 cDNA. (
  • Directed evolution and biophysical characterization of a full-length, soluble, human caveolin-1 variant. (
  • Caveolin-1 and caveolin-2 are widely present in a variety of normal human cells, and caveolin-3 is expressed specifically in muscle. (
  • E.coli-derived human Caveolin-1 recombinant protein (Position: G4-I178). (
  • Human Caveolin-1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Caveolin-1, respectively. (
  • STIM1 overexpression substantially enhanced SOCE: an effect only partially reversed by Cav-1 siRNA. (
  • Cav-1 overexpression was frequently confirmed in advanced cancer stages and positively associated with ABC transporters, cancer stem cell populations, aerobic glycolysis activity and autophagy. (
  • On the other hand, microarray analysis of HT29 and HT29 MTX resistant cells unveiled overexpression of caveolin 1, enolase 2 and PKCα genes in resistant cells without concomitant copy number gain. (
  • Combined treatments targeting siRNA inhibition of caveolin 1 and overexpression of E-cadherin markedly reduced cell viability in both sensitive and MTX-resistant HT29 cells. (
  • incubated at room temperature for 1.5 hours) with sh-Control and sh-Caveolin-1 transfected HeLa cells. (
  • In the TGN caveolin-1/-2 heterooligomers are sorted into basolateral vesicles, whereas larger caveolin-1 homooligomers are targeted to the apical side. (
  • We further demonstrate that large caveolin-1 complexes are present in apical transport vesicles. (
  • Induction of caveolin during adipogenesis and association of GLUT4 with caveolin-rich vesicles. (
  • Conclusions Caveolin-1 downregulation in leucocytes contributes to fibrotic lung disease, highlighting caveolin-1 as a promising therapeutic target in scleroderma. (
  • In vivo administration of a cell-permeable Cav-1 mimetic peptide was previously shown to prevent the development of monocrotaline-induced pulmonary artery medial hypertrophy, PH and right ventricular hypertrophy in rats. (
  • Indeed, downregulation of caveolin-1 leads to less efficient migration in vitro . (
  • Caveolin-1 scaffolding domain peptide prevents hyperoxia-induced airway remodeling in a neonatal mouse model. (
  • Significance Statement The caveolin-1 scaffolding domain peptide (CSD) reverses aging-associated fibrosis, microvascular leakage, and organ dysfunction in the heart, kidneys, and brain via a mechanism that involves the suppression of the activity of multiple tyrosine kinases, suggesting that CSD can be developed as a treatment for a wide range of diseases found primarily in the aged. (
  • A permeable competing peptide resembling the Cav-1 scaffolding domain bound to Dsg2, disrupted normal Dsg2 staining and interfered with the integrity of epithelial sheets in vitro. (
  • Therefore, we investigated the effect of in vivo administration of a Cav-1 mimetic peptide on the development of monocrotaline (MCT)-induced PH. (
  • The methods were used including examination of neurological deficit scores according to Longa's scale, measurement of blood-brain barrier permeability through Evans Blue content, in situ immunofluorescent detection of caveolin-1 in brains, western blot analysis of caveolin-1 in brains, and in situ zymography for measuring matrix metalloproteinase-2/9 activity in brains. (
  • In Western blot a band is observed ~23 kDa, representing the Caveolin 1 protein. (
  • In this study we used luciferase, western blot and quantitative PCR analysis to study Caveolin-1 as a direct target of miR-510. (
  • Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F) and ICC. (
  • Five μl (1-3 μg/μl) of mRNA were reverse transcribed to generate cDNA using Superscript II and random hexamers (Life Technologies, Inc.) according to manufacturers' protocols. (
  • The presence of CAV-1 protein was detected in VAT and SAT by immunohistochemistry. (
  • Statistical analysis was performed on the immunohistochemistry results for the tissue slices to investigate the correlation of caveolin-1 with the prostate cancer pathological grade, clinical stage, and preoperative serum PSA level to provide new information for the clinical diagnosis and treatment of prostate cancer. (
  • All these interactions are through a caveolin-scaffolding domain (CSD) within caveolin-1 molecule. (
  • Mechanistically, EFs activated Cav-1 and the downstream signaling molecule STAT3. (
  • Cytokine treatment of normal monocytes caused adoption of the scleroderma phenotype (low caveolin-1, high CXCR4 and MMP-9 and signalling molecule hyperactivation). (
  • With caveolin-1 deficiency, encephalitogenic T lymphocytes processed less efficient trafficking into CNS parenchyma, probably a sequence of declined expressions of adhesion molecule ICAM-1 and VCAM-1 in the white matter of CNS tissues. (
  • Furthermore, the association of Dsg2 with Cav-1 may provide a mechanism for regulating mitogenic signaling and modulating the cell-surface presentation of an important adhesion molecule, both of which could contribute to malignant transformation and tumor progression. (
  • In addition, migration assays showed that Caveolin-1 reduction increased RPE cell migration abilities. (
  • Mutant beta(3a)-ARs (F389A/Y392A/F398A or P384S/F389A) promoted PTX-sensitive cAMP responses, and in situ proximity assays demonstrated an association between caveolin-1 and the wild type beta(3a)-AR but not the mutant receptors. (
  • Furthermore, pharmacological inhibition of STAT3 significantly reduced the electrotactic response, while rescue of STAT3 activation in Cav-1 knock-down cells restored electrotaxis. (
  • Interestingly, our results indicated that only the incidence of caveolin-1 staining was significantly different between these two ethnic/racial groups of prostate cancer patients. (
  • Caveolin-1 manifestation levels were not significantly different among the FTC, BA, and control organizations. (
  • We Rabbit polyclonal to ARHGAP21 compared the age, thyroid function, thyroid autoantibodies, and caveolin-1 manifestation status among these 4 organizations and found that Feet3, TG-Ab, and caveolin-1-positive manifestation were significantly different between the PTC and control organizations. (
  • There were significantly more F4/80 positive staining cells at day 3 and day 14 in the wild-type injured kidney as compared to the caveolin-1 knockout mouse. (
  • The immunogen of 66067-1-Ig is Caveolin-1 Fusion Protein expressed in E. coli. (
  • Oligomers can also be produced from in vitro-synthesized caveolin-1 after translocation into microsomal membranes. (
  • Sprys are tightly associated with caveolin-1 and membranes. (
  • B) mSpry-1 is tightly associated with membranes. (
  • Caveolin-1 and cancer metabolism in the tumor microenvironment: markers, models, and mechanisms. (
  • Conclusion- Our studies demonstrate that p190RhoGAP links integrins and caveolin-1/caveolae to RhoA in a mechanotransduction cascade that participates in endothelial adaptation to flow. (
  • Rescuing caveolin-1 inhibits proliferative pathways and attenuates PH. (
  • 2011). 'RNA Interference of Caveolin-1 Via Lentiviral Vector Inhibits Growth of Hypopharyngeal Squamous Cell Carcinoma FaDu Cells In Vitro and In Vivo', Asian Pacific Journal of Cancer Prevention , 12(2), pp. 397-401. (
  • Transformed cells normally contain reduced or no caveolin-1. (
  • In contrast, increased caveolin-1 immunoreactivity both in number and intensity was detected in primary lung adenocarcinoma cells as well as in cancer cells that metastasized to regional lymph nodes from the cases diagnosed as advanced lung adenocarcinoma with nodal metastases. (
  • We further revealed that up-regulated caveolin-1 in CL cells is necessary for mediating filopodia formation, which may enhance the invasive ability of lung adenocarcinoma cells. (
  • Moderate transcripts were seen in BEAS-2B cells, absent or low in CL1-0 and CL1-1 cells, and abundant in CL1-5 and CL1-5F4 cells. (
  • The arrows indicate that caveolin-1 was overexpressed in CL1-5 and CL1-5F4 cells. (
  • Positive caveolin-1 staining was detected in cancer cells in a lymph node with metastatic lung adenocarcinoma. (
  • Knocking down Cav-1 using shRNA in HEK cells expressing the familial AD-linked APPswe mutant variant upregulates APP, APP carboxyl terminal fragments, and Aβ levels. (
  • Recently, we reported that cav-1 is secreted by androgen-insensitive prostate cancer cells, and we detected, by Western blotting, cav-1 in the high-density lipoprotein 3 fraction of serum specimens from patients with prostate cancer. (
  • In this paper we have analyzed the routing and biochemical characteristics of endogenous, newly synthesized caveolin-1 and -2 in MDCK cells. (
  • Gao M, Sun L, Liu YL, Xie JW, Qin L, Xue J, Wang YT, Guo KM, Ma MM and Li XY: Reduction of glyoxalase 1 (GLO1) aggravates cerebrovascular remodeling via promoting the proliferation of basilar smooth muscle cells in hypertension. (
  • In addition, the percentage of caveolin-1-positive prostate cancer cells was also higher in moderately differentiated (Gleason score 6) prostate cancer patients in AA versus whites. (
  • These results indicated that Caveolin-1 in RPE cells prevents PVR by blocking EMT. (
  • Renal MCDK distal tubular, HK2 proximal tubular epithelial cells and HEK293T renal embryonic cells were treated with 1 mM hydrogen peroxide. (
  • The serum TSH level was associated with caveolin-1 manifestation in thyroid epithelial cells. (
  • The control group was defined as individuals with paranodular thyroid cells located 1 cm away from a BA. (
  • We found that the level of Cav-1 was up-regulated rapidly in cells treated with ionizing radiation. (
  • Conclusion/Significance: Our results indicate that Cav-1 may play a critical role in sensing genotoxic stress and in orchestrating the response of cells to DNA damage through regulating the important molecules involved in maintaining genomic integrity. (
  • C ) Effect of the CAV-1 plasmid (2 μg/ml) on protecting the CR-A549 and CR-PC9 cells that were co-treated with cisplatin (8 μM) and miR-204 (50 pmol/ml). (
  • We provide functional evidences indicating that caveolin 1 and E-cadherin, deregulated in MTX resistant cells, may play a critical role in cell survival and may constitute potential targets for coadjuvant therapy. (
  • Here, we present evidence that the caveolin-1 protein is involved in the inhibition of spheroid formation, when confluent monolayers are exposed to microgravity. (
  • Caveolin-1 immunoreactivity in lung adenocarcinoma and ipsilateral hilar/peribronchial lymph nodes with tumor metastases. (
  • Negative or faint staining of caveolin-1 in the primary lung adenocarcinoma ( arrow ). (
  • Results Leucocyte caveolin-1 is important in lung fibrosis. (
  • We are testing our hypothesis that genetic variation at the cav-1 locus is a determinant of vascular phenotypes in hypertensive subjects. (
  • We evaluated whether the degree of negative remodeling in MMD patients was associated with RNF213 polymorphism, caveolin-1 levels, or various clinical and vascular risk factors. (
  • Reverse transcription‑quantitative PCR, western blotting and immunofluorescence staining were used to assess the mechanism of cav‑1/Notch1 involved in hypertensive vascular remodeling. (
  • Overexpressed Spry-1 and -2 inhibit fibroblast growth factor- and vascular endothelial growth factor-induced proliferation and differentiation by repressing pathways leading to p42/44 mitogen-activating protein (MAP) kinase activation. (
  • Indeed, recent findings show that integrin/vascular endothelial growth factor receptor 2 15 and PECAM-1/vascular endothelial-cadherin 16 associations form important mechanosignaling complexes. (
  • Our results indicate that serum cav-1 has the power to differentiate between prostate cancer and benign prostatic hyperplasia patients and the potential to be an important biomarker for prostate cancer. (
  • Additional studies to test the potential of serum cav-1 as a diagnostic and/or prognostic marker in prostate cancer are warranted. (
  • The serum caveolin-1 (ng/mL) level was lower in MMD patients than in controls (0.47±0.29 vs. 0.86±0.68, P =0.034). (
  • Cav-1 was tied to various stresses including radiotherapy, fluid shear and oxidative stresses and ultraviolet exposure, and interacted with stress signals such as AMP-activated protein kinase. (
  • In this mini-review, we characterize the functional role of caveolin-1 in multiple myeloma, and present the preclinical rationale for novel potential therapeutic approaches targeting caveolin-1 in multiple myeloma. (
  • The current review covers the relationship of Cav-1 and HIV in respect to viral replication, persistence, and the potential role in pathogenesis. (
  • Our findings suggest that caveolin-1 may play a major role in arterial negative remodeling in MMD patients. (
  • Thus, we suggest that caveolin-1 homooligomers play a role in apical transport. (
  • Ma X, Liu L, Nie W, Li Y, Zhang B, Zhang J and Zhou R: Prognostic role of caveolin in breast cancer: A meta-analysis. (
  • We also examined the role of Caveolin-1 in the pathogenesis of PVR. (
  • This study aimed to explore the role of Cav-1 in CRC and the associated mechanisms. (
  • These results suggest that Cav-1 plays a role in maintaining ion and fluid homeostasis and preventing retinal edema. (
  • A role for caveolin-1 in desmoglein binding and desmosome dynamics. (
  • and Mahoney, M G, "A role for caveolin-1 in desmoglein binding and desmosome dynamics. (
  • It has been shown that caveolin-1 plays a potential role as a diagnostic and prognostic biomarker in various cancer types. (
  • Methodology/Principal Findings: In the present study we sought to explore the role of Cav-1 in response toDNAdamage and the mechanism involved. (
  • Role of the miR-204/CAV-1 axis in regulating cisplatin sensitivity in NSCLC. (
  • Caveolin-1 has also been shown to play a role in the integrin signaling. (
  • The tyrosine phosphorylated form of caveolin-1 colocalizes with focal adhesions , suggesting a role for caveolin-1 in migration . (
  • A large set of HIF target genes has been identified as being critical in the control of metabolism, cell proliferation/survival, angiogenesis, and iron uptake ( 1 ). (
  • Caveolin-1 in SMC becomes proliferative, participating in cell proliferation, cell migration and worsening of disease. (
  • Multivariate analysis considering caveolin-1 immunoreactivity in addition to the established prognostic parameters such as pT stage, pN in these patients confirmed that caveolin-1 is an independent functional predictor of poor survival. (
  • In turn, rescue of Cav-1 levels restores APP metabolism. (
  • Therefore, the present study aimed to investigate the mechanism underlying Cav‑1 in hypertension. (
  • Recent studies have proved that caveolin-1 is associated with the incidence and progression of prostate cancer [ 7 ], but the specific mechanism is not clear. (
  • COX-2 is naturally inhibited by calcitriol (the active form of Vitamin D). Both the peroxidase and PTGS activities are inactivated during catalysis by mechanism-based, first-order processes, which means that PGHS-2 peroxidase or PTGS activities fall to zero within 1-2 minutes, even in the presence of sufficient substrates. (
  • This may suggest that depletion of Cav-1 in T2DM underlies, at least in part, the development of AD and imply that restoration of Cav-1 may be a therapeutic target for diabetic-associated sporadic AD. (
  • This study unravels signals underlying LOAD and suggests that restoration of Cav-1 may be an effective therapeutic target. (
  • Future studies exploring caveolin-1 as a therapeutic target in MMD are warranted. (
  • Taken together, the results indicate caveolin-1 as an active modulator of lymphocyte trafficking into CNS and may be a potential therapeutic target for neuroinflammatory diseases. (
  • Interestingly, our results indicate that FASN fails to be upregulated in Cav-1 -/- tumors. (
  • Importantly, the tumors examined were the same morphological grade, but Cav-1 -/- tumors were dramatically smaller and did not metastasize efficiently. (
  • In vitro studies suggested that HER2 and caveolin-1 are not coregulated, which was supported by IHC analysis in patient tumors. (