PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Caveolae: Endocytic/exocytic CELL MEMBRANE STRUCTURES rich in glycosphingolipids, cholesterol, and lipid-anchored membrane proteins that function in ENDOCYTOSIS (potocytosis), transcytosis, and SIGNAL TRANSDUCTION. Caveolae assume various shapes from open pits to closed vesicles. Caveolar coats are composed of CAVEOLINS.BooksPublishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.MEDLINE: The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).Serial Publications: Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)Membrane Microdomains: Detergent-insoluble CELL MEMBRANE components. They are enriched in SPHINGOLIPIDS and CHOLESTEROL and clustered with glycosyl-phosphatidylinositol (GPI)-anchored proteins.Endocytosis: Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.Clathrin: The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. Each molecule of clathrin consists of three light chains (CLATHRIN LIGHT CHAINS) and three heavy chains (CLATHRIN HEAVY CHAINS) that form a structure called a triskelion. Clathrin also interacts with cytoskeletal proteins.Caveolin 1: A tyrosine phosphoprotein that plays an essential role in CAVEOLAE formation. It binds CHOLESTEROL and is involved in LIPIDS transport, membrane traffic, and SIGNAL TRANSDUCTION.Caveolins: The main structural proteins of CAVEOLAE. Several distinct genes for caveolins have been identified.beta-Cyclodextrins: Cyclic GLUCANS consisting of seven (7) glucopyranose units linked by 1,4-glycosidic bonds.Notochord: A cartilaginous rod of mesodermal cells at the dorsal midline of all CHORDATE embryos. In lower vertebrates, notochord is the backbone of support. In the higher vertebrates, notochord is a transient structure, and segments of the vertebral column will develop around it. Notochord is also a source of midline signals that pattern surrounding tissues including the NEURAL TUBE development.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Caveolin 2: Caveolin 2 is a binding partner of CAVEOLIN 1. It undergoes tyrosine phosphorylation by C-SRC PROTEIN PP60 and plays a regulatory role in CAVEOLAE formation.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.3T3-L1 Cells: A continuous cell line that is a substrain of SWISS 3T3 CELLS developed though clonal isolation. The mouse fibroblast cells undergo an adipose-like conversion as they move to a confluent and contact-inhibited state.Polymorphism, Genetic: The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Muscle, Smooth: Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed)Urinary Bladder: A musculomembranous sac along the URINARY TRACT. URINE flows from the KIDNEYS into the bladder via the ureters (URETER), and is held there until URINATION.Muscle, Smooth, Vascular: The nonstriated involuntary muscle tissue of blood vessels.Relaxation: Activity which reduces the feelings of tension and the effects of STRESS, PHYSIOLOGICAL.Myocytes, Smooth Muscle: Non-striated, elongated, spindle-shaped cells found lining the digestive tract, uterus, and blood vessels. They are derived from specialized myoblasts (MYOBLASTS, SMOOTH MUSCLE).Respiratory Syncytial Viruses: A group of viruses in the PNEUMOVIRUS genus causing respiratory infections in various mammals. Humans and cattle are most affected but infections in goats and sheep have also been reported.Lipothrixviridae: Family of enveloped, lipid-containing, filamentous DNA viruses that infect ARCHAEA.Acidianus: A genus of facultatively anaerobic coccoid ARCHAEA, in the family SULFOLOBACEAE. Cells are highly irregular in shape and thermoacidophilic. Lithotrophic growth occurs aerobically via sulfur oxidation in some species. Distribution includes solfataric springs and fields, mudholes, and geothermically heated acidic marine environments.Respiratory Syncytial Virus Infections: Pneumovirus infections caused by the RESPIRATORY SYNCYTIAL VIRUSES. Humans and cattle are most affected but infections in goats and sheep have been reported.Respiratory Syncytial Virus, Human: The type species of PNEUMOVIRUS and an important cause of lower respiratory disease in infants and young children. It frequently presents with bronchitis and bronchopneumonia and is further characterized by fever, cough, dyspnea, wheezing, and pallor.Stem Cell Research: Experimentation on STEM CELLS and on the use of stem cells.Glutaral: One of the protein CROSS-LINKING REAGENTS that is used as a disinfectant for sterilization of heat-sensitive equipment and as a laboratory reagent, especially as a fixative.Fixatives: Agents employed in the preparation of histologic or pathologic specimens for the purpose of maintaining the existing form and structure of all of the constituent elements. Great numbers of different agents are used; some are also decalcifying and hardening agents. They must quickly kill and coagulate living tissue.Pinocytosis: The engulfing of liquids by cells by a process of invagination and closure of the cell membrane to form fluid-filled vacuoles.Freeze Fracturing: Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.Endothelial Cells: Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Gene Transfer Techniques: The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.Polyethyleneimine: Strongly cationic polymer that binds to certain proteins; used as a marker in immunology, to precipitate and purify enzymes and lipids. Synonyms: aziridine polymer; Epamine; Epomine; ethylenimine polymer; Montrek; PEI; Polymin(e).Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Genetic Therapy: Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Nanoparticles: Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.Suppressor of Cytokine Signaling Proteins: A family of structurally related proteins that are induced by CYTOKINES and negatively regulate cytokine-mediated SIGNAL TRANSDUCTION PATHWAYS. SOCS proteins contain a central SH2 DOMAIN and a C-terminal region of homology known as the SOCS box.Ubiquitin-Protein Ligases: A diverse class of enzymes that interact with UBIQUITIN-CONJUGATING ENZYMES and ubiquitination-specific protein substrates. Each member of this enzyme group has its own distinct specificity for a substrate and ubiquitin-conjugating enzyme. Ubiquitin-protein ligases exist as both monomeric proteins multiprotein complexes.Manuscripts as Topic: Compositions written by hand, as one written before the invention or adoption of printing. A manuscript may also refer to a handwritten copy of an ancient author. A manuscript may be handwritten or typewritten as distinguished from a printed copy, especially the copy of a writer's work from which printed copies are made. (Webster, 3d ed)Manuscripts, MedicalResearch: Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)Biogenesis: The origin of life. It includes studies of the potential basis for life in organic compounds but excludes studies of the development of altered forms of life through mutation and natural selection, which is BIOLOGICAL EVOLUTION.

Presence of oxidized cholesterol in caveolae uncouples active platelet-derived growth factor receptors from tyrosine kinase substrates. (1/744)

Platelet-derived growth factor receptor beta (PDGFRbeta) in fibroblasts is concentrated in caveolae where it controls the tyrosine phosphorylation of multiple proteins. Caveolae are enriched in cholesterol and sphingolipids, but the role of these lipids in PDGFR signal transduction is unknown. We report that introduction of cholest-4-en-3-one into caveolae membranes uncouples PDGFR autophosphorylation from tyrosine phosphorylation of neighboring proteins. Cholest-4-en-3-one appears to interfere with the normal interaction between PDGFR and its partners. The results suggest that tightly packed caveolae lipids form a membrane platform that functions as a lipid scaffold for organizing the molecular interactions of multiple signaling pathways.  (+info)

Agonist-modulated targeting of the EDG-1 receptor to plasmalemmal caveolae. eNOS activation by sphingosine 1-phosphate and the role of caveolin-1 in sphingolipid signal transduction. (2/744)

Plasmalemmal caveolae are membrane microdomains that are specifically enriched in sphingolipids and contain a wide array of signaling proteins, including the endothelial isoform of nitric-oxide synthase (eNOS). EDG-1 is a G protein-coupled receptor for sphingosine 1-phosphate (S1P) that is expressed in endothelial cells and has been implicated in diverse vascular signal transduction pathways. We analyzed the subcellular distribution of EDG-1 in COS-7 cells transiently transfected with cDNA constructs encoding epitope-tagged EDG-1. Subcellular fractionation of cell lysates resolved by ultracentrifugation in discontinuous sucrose gradients revealed that approximately 55% of the EDG-1 protein was recovered in fractions enriched in caveolin-1, a resident protein of caveolae. Co-immunoprecipitation experiments showed that EDG-1 could be specifically precipitated by antibodies directed against caveolin-1 and vice versa. The targeting of EDG-1 to caveolae-enriched fractions was markedly increased (from 51 +/- 11% to 93 +/- 14%) by treatment of transfected cells with S1P (5 microm, 60 min). In co-transfection experiments expressing EDG-1 and eNOS cDNAs in COS-7 cells, we found that S1P treatment significantly and specifically increased nitric-oxide synthase activity, with an EC(50) of 30 nm S1P. Overexpression of transfected caveolin-1 cDNA together with EDG-1 and eNOS markedly diminished S1P-mediated eNOS activation; caveolin overexpression also attenuated agonist-induced phosphorylation of EDG-1 receptor by >90%. These results suggest that the interaction of the EDG-1 receptor with caveolin may serve to inhibit signaling through the S1P pathway, even as the targeting of EDG-1 to caveolae facilitates the interactions of this receptor with ligands and effectors that are also targeted to caveolae. The agonist-modulated targeting of EDG-1 to caveolae and its dynamic inhibitory interactions with caveolin identify new points for regulation of sphingolipid-dependent signaling in the vascular wall.  (+info)

Scavenger receptor BI transfers major lipoprotein-associated phospholipids into the cells. (3/744)

The phospholipids of lipoproteins can be transferred to cells by an endocytosis-independent uptake pathway. We analyzed the role of scavenger receptor BI (SR-BI) for the selective cellular phospholipid import. Human monocytes rapidly acquired the pyrene (py)-labeled phospholipids sphingomyelin (SM), phosphatidylcholine, and phosphatidylethanolamine from different donors (low and high density lipoproteins (LDL, HDL), lipid vesicles). The anti-SR-BI antibody directed against the extracellular loop of the membrane protein lowered the cellular import of the phospholipids by 40-80%. The phospholipid transfer from the lipid vesicles into the monocytes was suppressed by LDL, HDL, and apoprotein AI. Transfection of BHK cells with the cDNA for human SR-BI enhanced the cellular import of the vesicle-derived py-phospholipids by 5-6-fold. In the case of the LDL donors, transfer of py-SM to the transfected cells was stimulated to a greater extent than the uptake of the other py-phospholipids. Similar differences were not observed when the vesicles and HDL were used as phospholipid donors. The concentration of LDL required for the half-maximal phospholipid import was close to the previously reported apparent dissociation constant for LDL binding to SR-BI. The low activation energy of the SR-BI-mediated py-phospholipid import indicated that the transfer occurs entirely in a hydrophobic environment. Disruption of cell membrane caveolae by cyclodextrin treatment reduced the SR-BI-catalyzed incorporation of py-SM, suggesting that intact caveolae are necessary for the phospholipid uptake. In conclusion, SR-BI mediates the selective import of the major lipoprotein-associated phospholipids into the cells, the transfer efficiency being dependent on the structure of the donor lipoprotein.  (+info)

Isoforms of caveolin-1 and caveolar structure. (4/744)

The relationship between caveolin-1 isoforms alpha and beta and caveolar ultrastructure was studied. By immunofluorescence microscopy of human fibroblasts, caveolae were observed as dots positive for caveolin-1, but many dots labeled by an antibody recognizing both isoforms (anti-alphabeta) were not labeled by another antibody specific for the alpha isoform (anti-alpha). Immunogold electron microscopy of freeze-fracture replicas revealed caveolae of different depths, and indicated that anti-alpha labeled deep caveolae preferentially over shallow ones, whereas anti-alphabeta labeled both forms with an equivalent frequency and intensity. The presence of the beta isoform in deep caveolae was confirmed by labeling epitope-tagged beta-caveolin. When made to be expressed in HepG2 cells lacking endogenous caveolins, the alpha isoform formed caveolar depressions efficiently, but the beta isoform hardly did so. Caveolae were also formed in cells expressing the two isoforms, but their frequency was variable among cells of the same clone. Coexpression of caveolin-1 and caveolin-2 caused more efficient formation of deep caveolae than caveolin-1 alone. The result indicates that the two isoforms of caveolin-1 have a different potential for forming caveolae structure, and more importantly, that deep and shallow caveolae may be diversified in their molecular composition.  (+info)

Ultrastructural and histochemical characterization of special muscle cells in the monkey small intestine. (5/744)

The ultrastructure, three-dimensional arrangement, and histochemical features of special muscle cells in the monkey small intestine were investigated. The cells formed a special layer separated from the main part by a connective tissue space along the submucosal surface of the circular muscle coat. Scanning electron microscopy using alkali maceration demonstrated this inner sublayer to be a continuous thin sheet consisting of irregularly-shaped muscle cells equipped with many cytoplasmic projections and caveolae. Other ultrastructural features included direct contact with interstitial cells, due to their close association with nerve fibers of the deep muscular plexus. Histochemical examination revealed significant alkaline phosphatase activity and immunoreactivity for vascular smooth muscle alpha actin in these muscle cells, whereas the ordinary circular muscle cells were immunopositive for enteric smooth muscle gamma actin. These findings suggest that the special muscle cells play an important role in regulating the radial stretch of the monkey small intestinal wall.  (+info)

Bradykinin activates the Janus-activated kinase/signal transducers and activators of transcription (JAK/STAT) pathway in vascular endothelial cells: localization of JAK/STAT signalling proteins in plasmalemmal caveolae. (6/744)

Bradykinin (BK) is an important physiological regulator of endothelial cell function. In the present study, we have examined the role of the Janus-activated kinase (JAK)/signal transducers and activators of transcription (STAT) pathway in endothelial signal transduction through the BK B2 receptor (B2R). In cultured bovine aortic endothelial cells (BAECs), BK activates Tyk2 of the JAK family of tyrosine kinases. Activation results in the tyrosine phosphorylation and subsequent nuclear translocation of STAT3. BK also activates the mitogen-activated p44 and p42 protein kinases, resulting in STAT3 serine phosphorylation. Furthermore, Tyk2 and STAT3 form a complex with the B2R in response to BK stimulation. Under basal conditions, Tyk2, STAT3 and the B2R are localized either partially or entirely in endothelial plasmalemmal caveolae. Following BK stimulation of BAECs, however, the B2R and STAT3 are translocated out of caveolae. Taken together, these data suggest that BK activates the JAK/STAT pathway in endothelial cells and that JAK/STAT signalling proteins are localized in endothelial caveolae. Moreover, caveolar localization of the B2R and STAT3 appears to be regulated in an agonist-dependent manner.  (+info)

Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway. (7/744)

Differential modes for beta(1)- and beta(2)-adrenergic receptor (AR) regulation of adenylyl cyclase in cardiomyocytes is most consistent with spatial regulation in microdomains of the plasma membrane. This study examines whether caveolae represent specialized subdomains that concentrate and organize these moieties in cardiomyocytes. Caveolae from quiescent rat ventricular cardiomyocytes are highly enriched in beta(2)-ARs, Galpha(i), protein kinase A RIIalpha subunits, caveolin-3, and flotillins (caveolin functional homologues); beta(1)-ARs, m(2)-muscarinic cholinergic receptors, Galpha(s), and cardiac types V/VI adenylyl cyclase distribute between caveolae and other cell fractions, whereas protein kinase A RIalpha subunits, G protein-coupled receptor kinase-2, and clathrin are largely excluded from caveolae. Cell surface beta(2)-ARs localize to caveolae in cardiomyocytes and cardiac fibroblasts (with markedly different beta(2)-AR expression levels), indicating that the fidelity of beta(2)-AR targeting to caveolae is maintained over a physiologic range of beta(2)-AR expression. In cardiomyocytes, agonist stimulation leads to a marked decline in the abundance of beta(2)-ARs (but not beta(1)-ARs) in caveolae. Other studies show co-immunoprecipitation of cardiomyocytes adenylyl cyclase V/VI and caveolin-3, suggesting their in vivo association. However, caveolin is not required for adenylyl cyclase targeting to low density membranes, since adenylyl cyclase targets to low buoyant density membrane fractions of HEK cells that lack prototypical caveolins. Nevertheless, cholesterol depletion with cyclodextrin augments agonist-stimulated cAMP accumulation, indicating that caveolae function as negative regulators of cAMP accumulation. The inhibitory interaction between caveolae and the cAMP signaling pathway as well as domain-specific differences in the stoichiometry of individual elements in the beta-AR signaling cascade represent important modifiers of cAMP-dependent signaling in the heart.  (+info)

Downmodulation of caveolin-1 expression in human ovarian carcinoma is directly related to alpha-folate receptor overexpression. (8/744)

Caveolin (cav-1) and the GPI-anchored alpha-folate receptor (alphaFR) are membrane proteins both found associated to caveolar structures. Several studies in tumor cells independently reported cav-1 downregulation and alphaFR overexpression. Here we analysed the expression of the two molecules in normal and tumor ovarian samples derived from fresh specimens and from cultured cell lines. Whereas normal ovary surface epithelial cells displayed only cav-1 expression, ovarian tumor surgical samples and cell lines (COR, IGROV1, OVCAR3 and OVCA432) displayed high alphaFR and low-level or no cav-1 expression, except those cell lines (SKOV3 and SW626) with the lower alphaFR expression. SKOV3, but not two alphaFR-negative non-ovarian cell lines, exhibited down-regulation of cav-1 expression following stable alphaFR cDNA transfection. Conversely, cav-1 transfection in IGROV1 cells led to downregulated alphaFR expression, together with formation of caveolar structures and reduction of growth capability. Moreover, cav-1 expression was induced in IGROV1 cells by transfection with intracellular anti-alphaFR antibodies to downmodulate alphaFR expression. In cav-1 transfected cells, transcriptional activity of the alphaFR-specific promoter P1 was reduced by 70% and an additional specific DNA-protein complex was identified by gel-shift assay, indicating that cav-1 expression influences alphaFR gene transcription. Together these results support the notion that alphaFR and cav-1 protein expression is reciprocally regulated in ovary cancer cells.  (+info)

*Plasma membrane Ca2+ ATPase

PMCA4 exists in caveolae. Isoform PMCA4b interacts with nitric oxide synthase and reduces synthesis of nitric oxide by that ...

*Endocytosis

Caveolae are small (approx. 50 nm in diameter) flask-shape pits in the membrane that resemble the shape of a cave (hence the ... Caveolae are the most common reported non-clathrin-coated plasma membrane buds, which exist on the surface of many, but not all ... Potocytosis is a form of receptor-mediated endocytosis that uses caveolae vesicles to bring molecules of various sizes into the ... Parton RG, Simons K (March 2007). "The multiple faces of caveolae". Nature Reviews Molecular Cell Biology. 8 (3): 185-94. doi: ...

*Congenital generalized lipodystrophy

One of the roles the PTRF product has it to stabilize and aid in formation of caveolae. Thus, the mechanism is similar to Type ... Parton, RG; Simons, K (2007). "The multiple faces of caveolae". Nature Reviews Molecular Cell Biology. 8 (3): 185-94. doi: ... 3, in that the caveolae are unable to properly form and carry out their role in lipid regulation in both. Types 3 and 4 are two ...

*Major capsid protein VP1

The exact mechanism of endocytosis varies depending on the virus, and some viruses use multiple mechanisms; caveolae-dependent ...

*Caveolin

... called caveolae. Oligomers of caveolin form the coat of these domains. Cells that lack caveolins also lack caveolae. Many ... Galbiati F, Razani B, Lisanti MP (October 2001). "Caveolae and caveolin-3 in muscular dystrophy". Trends Mol Med. 7 (10): 435- ... In molecular biology caveolins are a family of integral membrane proteins that are the principal components of caveolae ... showing that neither the caveolins nor the caveolae are essential in embryonic development or reproduction of these animals. ...

*Caveolin 3

This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell ... Caveolin-3 associates with the cardiac sodium-calcium exchanger (NCX) in caveolae of cardiac myocytes. This association occurs ... Disruption of caveolin-3 disturbs the structure of cardiac caveolae and blocks atrial natriuretic peptide (ANP) expression, a ... Gratton JP, Bernatchez P, Sessa WC (2004). "Caveolae and caveolins in the cardiovascular system". Circ. Res. 94 (11): 1408-17. ...

*FLOT1

... encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. FLOT1 has ... Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. ... "Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins". J. Biol. Chem. ...

*Caveolin 1

Ikezu, T; Trapp B D; Song K S; Schlegel A; Lisanti M P; Okamoto T (April 1998). "Caveolae, plasma membrane microdomains for ... The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. ... "Entrez Gene: CAV1 caveolin 1, caveolae protein, 22kDa". Li, Shengwen; Okamoto T; Chun M; Sargiacomo M; Casanova JE; Hansen SH; ... Implications for the interaction of caveolin with caveolae-associated proteins". J. Biol. Chem. 272 (10): 6525-33. doi:10.1074/ ...

*ENTPD1

"Palmitoylation targets CD39/endothelial ATP diphosphohydrolase to caveolae". The Journal of Biological Chemistry. 275 (3): 2057 ...

*PTRF

... (Cavin1) forms trimers with Cavin2 and Cavin3 in caveola formation and has been shown to interact with other membrane ... Kovtun O, Tillu VA, Ariotti N, Parton RG, Collins BM (April 2015). "Cavin family proteins and the assembly of caveolae". ... PTRF (Cavin1) has been shown to be crucial for caveola formation and function. Termination of RNA polymerase I catalyzed ... at the surface of caveolae in human adipocytes". The Biochemical Journal. 383 (Pt 2): 237-48. doi:10.1042/BJ20040647. PMC ...

*DNA vaccination

December 1, 1992). "Expression of naked plasmids by cultured myotubes and entry of plasmids into T tubules and caveolae of ... Using electron microscopy, it was proposed that DNA uptake was facilitated by caveolae (or, non-clathrin coated pits). However ... sequestration and transport of small molecules by caveolae". Science. 255 (5043): 410-1. doi:10.1126/science.1310359. PMID ...

*Lipid raft

SV40 may recruit more caveolae from the cytoplasm or even new caveolae formed at the site of entry. A cascade of virus-induced ... Caveolae, on the other hand, are flask shaped invaginations of the plasma membrane that contain caveolin proteins and are the ... Caveolae Cell membrane Cholesterol Lipid microdomain Membrane proteins Sphingomyelin Thomas, Sunil; Preda-Pais, Anca; Casares, ... Planar rafts contain flotillin proteins and are found in neurons where caveolae are absent. Both types have similar lipid ...

*SDPR

1998). "Targeting of Protein Kinase Cα to Caveolae". J. Cell Biol. 141 (3): 601-10. doi:10.1083/jcb.141.3.601. PMC 2132740 . ...

*PACSIN2

Pacsin 2 is involved in the formation of caveolae. PACSIN2 has been shown to interact with: Fas ligand, PACSIN1, and PACSIN3. ... update on the structure and function of caveolae". Current Opinion in Cell Biology. 29: 99-106. doi:10.1016/j.ceb.2014.04.011. ...

*Endothelial NOS

With the binding of eNOS to caveolae, the enzyme is inactivated due to the strong and direct interaction of eNOS with caveolin- ... Lisanti MP, Scherer PE, Tang Z, Sargiacomo M (Jul 1994). "Caveolae, caveolin and caveolin-rich membrane domains: a signalling ... Importantly, eNOS is attached by myristoylation and palmitoylation to caveolae, a pocket-like invagination on the membrane rich ...

*Channelosome

The channelosome is frequently clustered within a lipid microdomain or caveolae. This collection of proteins may be involved ...

*EN1 (gene)

1997). "Association of Engrailed homeoproteins with vesicles presenting caveolae-like properties". Development. 124 (10): 1865- ...

*MAP3K2

Pelkmans L, Zerial M (Jul 2005). "Kinase-regulated quantal assemblies and kiss-and-run recycling of caveolae". Nature. 436 ( ...

*Low-density lipoprotein receptor-related protein 8

Riddell DR, Sun XM, Stannard AK, Soutar AK, Owen JS (2001). "Localization of apolipoprotein E receptor 2 to caveolae in the ...

*Phospholipase D1

Kim JH, Han JM, Lee S, Kim Y, Lee TG, Park JB, Lee SD, Suh PG, Ryu SH (1999). "Phospholipase D1 in caveolae: regulation by ...

*List of diminutives by language

L caveola), mail (1320; L macula), pill (1400), quail (c. 1300; ML quaccula), rail (1320; L regula), rail (1460; VL rasculum), ...

*Marilyn Farquhar

Microtubules and actin microfilaments regulate lipid raft/caveolae localization of adenylyl cyclase signaling components. J ...

*Amyloid precursor protein

Ikezu T, Trapp BD, Song KS, Schlegel A, Lisanti MP, Okamoto T (Apr 1998). "Caveolae, plasma membrane microdomains for alpha- ...

*Telocyte

0.24 μm; Podoms accommodate: mitochondria, (rough) endoplasmic reticulum, caveolae, a trio called 'Ca2+-uptake/release units'. ... C. High resolution image illustrating in detail multiple mitochondria, endoplamic reticulum cisterne and caveolae (arrow). ... endoplasmic reticulum and caveolae - the so-called "Ca2+ uptake/release units". The concept of TC was promptly adopted by other ...
TY - JOUR. T1 - Circulating cardiovascular disease risk factors and signaling in endothelial cell caveolae. AU - Mineo, Chieko. AU - Shaul, Philip W.. PY - 2006/4/1. Y1 - 2006/4/1. N2 - Caveolae are a subset of lipid rafts that are prevalent on the plasma membrane of endothelial cells. They compartmentalize signal transduction molecules which regulate multiple endothelial functions including the production of nitric oxide (NO) by the caveolae resident enzyme endothelial NO synthase (eNOS). Recent studies have demonstrated that circulating factors known to modify cardiovascular disease risk regulate signaling in endothelial cell caveolae. In particular, high density lipoprotein (HDL) maintains the lipid environment in caveolae, thereby promoting the retention and function of eNOS in the domain, and it also causes direct activation of eNOS via scavenger receptor type BI (SR-BI)-induced kinase signaling. Estrogen binding to estrogen receptors (ER) in caveolae also activates eNOS, and this occurs ...
article{364f9c3e-a101-48eb-bf5b-ea6e0c163acd, abstract = {,p,Cavins belong to a family of proteins that contribute to the formation of caveolae, which are membrane organelles with functional roles in muscle and fat. Here, we investigate the effect of cavin-3 ablation on vascular and urinary bladder structure and function. Arteries and urinary bladders from mice lacking cavin-3 (knockout: KO) and from controls (wild type: WT) were examined. Our studies revealed that the loss of cavin-3 resulted in ∼40% reduction of the caveolae protein cavin-1 in vascular and bladder smooth muscle. Electron microscopy demonstrated that the loss of cavin-3 was accompanied by a reduction of caveolae abundance by 40-45% in smooth muscle, whereas the density of caveolae in endothelial cells was unchanged. Vascular contraction in response to an α,sub,1,/sub,-adrenergic agonist was normal but nitric-oxide-dependent relaxation was enhanced, in parallel with an increased relaxation on direct activation of soluble ...
Caveolae are plasma membrane microdomains that localise receptors and signalling intermediates within an environment where they can efficiently trigger downstream events. While present in most cells types, they are particularly abundant in vascular smooth muscle cells and endothelial cells (ECs), where they regulate low density lipoprotein trans-cytosis, NO production and inflammation. Cavin-1 is a protein required for caveolae maturation, such that its deletion results in no detectable caveolae and the down-regulation of "caveolin" scaffolding proteins. However, the cellular processes regulating cavin-1 function are poorly understood. Proteomic screening and biochemical experiments have identified cavin-1 as a novel interacting protein of the cytokine-inducible E3 ubiquitin ligase component "suppressor of cytokine signalling-3" (SOCS3), a key inhibitor of IL-6-mediated pro-inflammatory signalling in ECs. We hypothesise d that the SOCS3/cavin-1 interaction may be important in controlling caveola ...
Caveolae. Caveolae were described in the fifties of the last century by P. Palade after observing animal tissues at transmission electron microscopy. They are small invaginations (45-80 nm) of the plasma membrane that can be observed in most of the eukaryotic cells. It was suggested that most of the caveolae become vesicles (but see below). Caveolae are abundant in endothelial cells, muscle cells and adipocytes. The membrane of the caveolae contains caveolin, as well as other integral proteins linked to glycosylphosphatidylinositol, many sphingolipids (sphingomyelin and glycosphingolipids), and is enriched in cholesterol. The presence of caveolin in a cell is enought to form caveolae. There are around 100 to 200 caveolin molecules in one caveola and there are different types of caveolin in one caveola. Caveolin 1 is expressed in smooth muscle cells and in most of the non muscle cells, and it is necessary for caveolae formation in these cells. Caveolin 2, which can be expressed in the same cells ...
Using a Polaron E7200 quick freeze unit, we investigated the distribution of vesicles (caveolae) bound to the plasma membrane of aortic endothelial cells from adult Sprague-Dawley rats. Counts of caveolae from replicas of rapidly frozen, unfixed samples were made and compared with counts from both aldehyde-fixed, conventionally frozen, and rapidly frozen aortic samples. Aldehyde-fixed samples prepared for freeze fracture by either of the freezing methods revealed a significantly greater number of caveolae than did unfixed, rapidly frozen samples. These findings suggest that the relative number of caveolae is artifactually increased by exposure to aldehyde fixatives. We conclude that previous estimates of the rates of pinocytotic activity in vascular endothelial cells may contain substantial errors based on overestimates of caveolae and underestimates of subplasmalemmal "free" vesicles. ...
Cell fractionation and immunocytochemistry have previously shown that PKCα is constitutively present in caveolae and that this is a major cell surface location for the enzyme (25). We used a solid phase binding assay that has successfully identified other membrane binding sites for cytosolic proteins (28) to determine if PKCα would bind to caveolae. PKCα bound with high affinity (binding was dependent on calcium) did not require the addition of either PMA, PS, or ATP, and only occurred at 37°C. PKCα did not bind to noncaveolae membranes, which contain ,90% of the plasma membrane protein starting material. The same specific interaction with caveolae was also detected in a solution binding assay. Caveolae, therefore, exhibit a PKCα binding activity that may be responsible for targeting the enzyme to this compartment.. We found that caveolae contained other members of the PKC enzyme family. Fractions from untreated cells contained PKCα only when calcium was present and PKCλ only when ...
The primary function of adipose tissue is to store energy in the form of triacylglycerol, which is hydrolyzed to fatty acids to supply other tissues with energy. While insulin promotes the storage of triacylglycerol, catecholamines stimulate its hydrolysis. The development of type II diabetes is strongly associated with obesity, indicating a role of triacylglycerol metabolism in the pathogenesis of diabetes. Caveolae are plasma membrane invaginations found in most cells but are highly abundant in adipocytes. Insulin receptors are localized in caveolae and their function depends on intact caveolae structures. In the present thesis work, mass spectrometry-based methodology allowed identification of a number of new proteins and their posttranslational modifications in caveolae of human adipocytes. Variable N-terminal acetylation and phosphorylation of caveolin-1α and caveolin-1β were identified, which might regulate the function of caveolae. The transcription regulator protein PTRF was identified ...
Plays an important role in caveolar biogenesis and morphology. Regulates caveolae morphology by inducing membrane curvature within caveolae (PubMed:19525939). Plays a role in caveola formation in a tissue-specific manner. Required for the formation of caveolae in the lung and fat endothelia but not in the heart endothelia. Negatively regulates the size or stability of CAVIN complexes in the lung endothelial cells. May play a role in targeting PRKCA to caveolae (By similarity).
Background: Muscle-restricted coiled-coil protein (MURC)/cavin-4 is a novel member of the cavin family that regulates caveolae function. Caveolae formation and function are also regulated by caveolin-3 in cardiomyocytes (CMs). Overexpression of caveolin-3 induces cardiac protection, and loss of caveolin-3 causes progressive cardiomyopathy. Mutations in MURC have been identified in patients with dilated cardiomyopathy. However, the role of MURC as a caveolar component protein remains unknown.. Methods and Results: To assess the role of MURC in the development of heart failure, MURC-knockout (KO) and wild-type (WT) mice were subjected to pressure overload by transverse aortic constriction (TAC). After TAC, WT mice developed overt heart failure and eccentric cardiac hypertrophy, whereas MURC-KO mice showed preserved cardiac function accompanied by attenuated cardiac hypertrophy. We then focused on β-adrenergic receptor (AR) signaling, because MURC was colocalized and associated with β1-AR. ...
In biology, caveolae (Latin for "little caves"; singular, caveola), which are a special type of lipid raft, are small (50-100 nanometer) invaginations of the plasma membrane in many vertebrate cell types, especially in endothelial cells, adipocytes and embryonic notochord cells. They were originally discovered by E. Yamada in 1955. These flask-shaped structures are rich in proteins as well as lipids such as cholesterol and sphingolipids and have several functions in signal transduction. They are also believed to play a role in mechanoprotection, mechanosensation, endocytosis, oncogenesis, and the uptake of pathogenic bacteria and certain viruses. Formation and maintenance of caveolae was initially thought to be primarily due to caveolin, a 21 kD protein. There are three homologous genes of caveolin expressed in mammalian cells: Cav1, Cav2 and Cav3. These proteins have a common topology: cytoplasmic N-terminus with scaffolding domain, long hairpin transmembrane domain and cytoplasmic C-terminus. ...
Cyclic nucleotide phosphodiesterases (PDEs) are important regulators of signal transduction processes mediated by cAMP and cGMP. One PDE family member, PDE3B, plays an important role in the regulation of a variety of metabolic processes such as lipolysis and insulin secretion. In this study, the cellular localization and the role of PDE3B in the regulation of triglyceride, cholesterol and glucose metabolism in hepatocytes were investigated. PDE3B was identified in caveolae, specific regions in the plasma membrane, and smooth endoplasmic reticulum. In caveolin-1 knock out mice, which lack caveolae, the amount of PDE3B protein and activity were reduced indicating a role of caveolin-1/caveolae in the stabilization of enzyme protein. Hepatocytes from PDE3B knock out mice displayed increased glucose, triglyceride and cholesterol levels, which was associated with increased expression of gluconeogenic and lipogenic genes/enzymes including, phosphoenolpyruvate carboxykinase, peroxisome ...
This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3 end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy. [provided by RefSeq, Nov 2009 ...
and forskolin-stimulated AC activity is increased by caveolin-1 knockdown or knockout, the results suggest that noninvolvement of Gαi or a GPCR coupled to Gαi. Alternatively, because caveolin-1 is a cholesterol-binding protein (Murata et al., 1995), limiting its expression could lower membrane cholesterol. This is not the case (Fig. 5). In fact, incubating wild-type or Cav-1 RNAi cells with 1 mg/ml exogenous cholesterol significantly decreased isoproterenol-stimulated cAMP accumulation (Fig. 5). Thus, removing membrane cholesterol does seem to enhance βAR/Gαs/adenylyl cyclase signaling (most likely by disrupting rafts/caveolae), and adding cholesterol has the opposite effect. Therefore, the elevated cAMP signaling due to loss of caveolin is independent of Gαi and cholesterol levels.. Lipid rafts/caveolae can influence both spatial organization at the membrane and intracellular trafficking of signaling molecules; both mechanisms could regulate the Gαs/adenylyl cyclase signaling cascade. ...
TY - JOUR. T1 - AMP-dependent kinase inhibits oxidative stress-induced caveolin-1 phosphorylation and endocytosis by suppressing the dissociation between c-Abl and Prdx1 proteins in endothelial cells. AU - Takeuchi, Kimio. AU - Morizane, Yuki. AU - Kamami-Levy, Cynthia. AU - Suzuki, Jun. AU - Kayama, Maki. AU - Cai, Wenyi. AU - Miller, Joan W.. AU - Vavvas, Demetrios G.. PY - 2013/7/12. Y1 - 2013/7/12. N2 - Caveolin-1 is the primary structural component of endothelial caveolae that is essential for transcellular trafficking of albumin and is also a critical scaffolding protein that regulates the activity of signaling molecules in caveolae. Phosphorylation of caveolin-1 plays a fundamental role in the mechanism of oxidant-induced vascular hyper permeability. However, the regulatory mechanism of caveolin-1 phosphorylation remains unclear. Here we identify a previously unexpected role for AMPKin inhibition of caveolin-1 phosphorylation under oxidative stress. A pharmacological activator of AMPK, ...
Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane. Recruits DNM2 to caveolae, and thereby plays a role in caveola-mediated endocytosis.
Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane. Recruits DNM2 to caveolae, and thereby plays a role in caveola-mediated endocytosis ...
The localization of ion channels to caveolae may modulate the function of the channels in multiple ways. For example, the precise lipid composition can potently regulate channel function, and dynamic changes in this composition underlie some forms of regulation. In addition, the caveolar localization of ion channels can provide compartmentalization of signaling networks, enabling rapid and specific regulation of the channels. Caveolar-localized signaling complexes composed of β2-AR, AC, Gαs, and Gαi have previously been observed in rat ventricular myocytes (9, 19), but the present study adds Cav1.2 and PP2A to the complex. Conversely, the molecules associated with Cav1.2 channels in the heart have not been extensively defined. Recent studies have identified AKAP15 and PKA as well as certain PKC isoforms in association with Cav1.2 in cardiac muscle (23, 24); however, whether these molecules are associated with the channels in caveolae is unknown. The present results add to the list of proteins ...
... - Caveolae are submicroscopic, plasma membrane pits that are abundant in many mammalian cell types. The past few years have seen a quantum leap in our.
Caveolin-1 (Cav-1) is a 21?kDa protein enriched in caveolae and continues to be implicated in oncogenic cell transformation CHC tumorigenesis and metastasis. response to chemotherapy and radiation and tumor growth. We found Cav-1 is definitely overexpressed in human being Personal computer cell lines mouse models and human being pancreatic tumors and is associated with worse tumor grade and clinical results. In Personal computer cell lines disruption/depletion of caveolae/Cav-1 reduces proliferation colony formation and invasion. Radiation and chemotherapy up-regulate Cav-1 manifestation while Cav-1 depletion induces both chemosensitization and radiosensitization through modified apoptotic and DNA restoration signaling. and and transgene ("KPC mouse") (Fig. 1B). Cav-1 manifestation was also tested on a cells microarray of 110 individuals with pancreatic malignancy and obtained semi-quantitatively for low versus high CHC manifestation. While Cav-1 is definitely virtually absent in pancreatic ...
Caveolae, the sphingomy-cholesterol enriched microdomains, form a stable lipid matrix that act as an ordered support for receptor-mediated signaling events. It has been proposed that caveola composition changes in response to adverse extracellular conditions [35,36]. Consistent with this hypothesis, we found in the current study that HG treatment led to a decrease in the number of caveolae on the surface of LECs. We also found reduced levels of caveolin-1 mRNA and protein in HG-treated LECs, indicating that caveolin-1 expression was down-regulated at the transcription level. Although it has been proposed that caveolin-1 functions as a message center that compartmentalizes anti- and pro-apoptotic signaling molecules on the cell surface to regulate apoptosis [17], its role in regulating apoptosis remains controversial. In the current study, we found the levels of caveolin-1 reduced and apoptosis rate increased in HG-treated LECs, indicating that caveolin-1 may inhibit apoptosis. However, ...
The Editors wish to note that a reader has raised questions about the integrity of certain figures in the foregoing articles listed below. We have been informed by Emory University of an ongoing review conducted by an inquiry committee at the University. We are awaiting the findings of the committee to determine the appropriate next steps.. 1) Zuo L, Ushio-Fukai M, Ikeda S, Hilenski L, and Alexander RW. Microtubules regulate angiotensin II type receptor and rac 1 localization in caveolae/lipid rafts: role in redox signaling. Arterioscler Thromb Vasc Biol. 2004;24:1223-1228.. 2) Zuo L, Ushio-Fukai M, Ikeda S, Hilenski L, Patrushev N, Alexander RW. Caveolin-1 is essential for activation of rac 1 and NAD(P)H oxidase after angiotensin II type 1 receptor stimulation in vascular smooth muscle cells: role in redox signaling and vascular hypertrophy. Arterioscler Thromb Vasc Biol. 2005;25;1824-1830.. ...
Obesity and overweight is an increasing health problem in the world. Besides reducing quality of life obesity is a great risk factor for development of diseases such as type 2 diabetes, cardiovascular disease, and stroke. The adipose tissue is a flexible organ that grows in size if the body is provided with excess energy. Adipocytes store the excess energy in the form of triacylglycerol. The incorporation of fat is stimulated by insulin, while catecholamines promote hydrolysis of triacylglycerol. In the membrane of adipocytes there are cave-like invaginations, caveolae, where proteins involved in insulin signalling, such as the insulin receptor are located. The focus of this thesis is on the function, and thereby the importance, of adipocytes and caveolae in metabolism.. The lipid content of the plasma membrane and caveolae was analyzed and compared to lipid phases determined in model membranes. The amount of different membrane lipids indicated heterogeneity of the caveolae, since there were not ...
Caveolae are specialised forms of lipid rafts in the plasma membrane of most cells. They form dynamic assemblies of sphingolipids and cholesterol containing scaffolding domains with different affinities for proteins resulting in a variety of functions [1]. The constitutive Cav-1 protein is distributed ubiquitously, while Cav-2 is usually associated with Cav-1 [3, 28]. Although Cav-3 is thought to be "muscle specific" and is expressed in striated muscles [3, 28, 29], we and others have found that Cav-3 is not expressed within human ASM [6, 30]. Recent studies have established that ASM caveolae contain a number of proteins important to [Ca2+]i regulation (e.g. agonist receptors, Ca2+ influx channels, and force regulatory proteins such as RhoA). In canine ASM, it has been established that caveolar-enriched membrane fractions express Cav-1, L-type Ca2+ channels and plasma membrane Ca2+ ATPase, but not SR proteins such as IP3R or RyR channels [31].. In ASM of different species, in addition to Ca2+ ...
View mouse Cavin3 Chr7:105480083-105482300 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
Received 6 November 2009. Accepted 1 December 2009. Uncorrected manuscript published online 3 December 2009. Published online 8 January 2010 ...
Introduction. Caveolin-1(Cav-1) is a multifunctional scaffolding protein essential structural constituent of caveolae, regulating multiple processes in cancer.. A growing body of evidence links elevated expression of Cav-1 to an aggressive phenotype of cancer cells and to a poor prognosis in several tumors. The molecular mechanisms underlying the pro-survival and tumor-promoting functions of Cav-1 and the down-stream mediators of Cav-1 functional role in metastatic cancer have jet to be fully identified. The aim of the present work was to prosecute the investigation on such molecular pathways in solid tumors, based on our recent data obtained in cell lines from SCLC (SCLC-R1) and NSCLC (RAL) and from glioblastoma (A-172) where we demonstrated an oncopromoter activity of Cav-1 through the activation of STAT3 signaling pathway. Here we present the data obtained in a cell line from osteosarcoma (143B), where Cav-1 was highly expressed.. Methods. Cav-1 was knocked-down by siRNA using the ...
Caveolae, Membrane, Endocytosis, Plasma, Plasma Membrane, Senescence, Binding Protein, Caveolin-1, Cellular Senescence, Fibroblasts, Insulin, Mutations, ATP, Mutation, and Blood
The scaffolding protein CAV1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to…
Expression of myocardin family genes correlate with expression of caveolae genes in humans.mRNA expression data was retrieved from the GTExPortal. TMM-normalize
Caveolae and rafts represent highly heterogeneous populations of functionally distinct membrane domains (Maxfield, 2002). Multiple raft-associated proteins may therefore serve to segregate and define raft domains that exhibit differential endocytic capacities. The existence of multiple caveolin-1 binding partners (Liu et al., 2002) implicates caveolin-1 as a scaffolding molecule that determines the cargo for caveolae/raft-dependent endocytosis. Caveolae/raft ligands are also internalized by other endocytic pathways. For instance, cholera, shiga, and anthrax toxins bind to cell surface raft domains and yet are internalized via clathrin-dependent pathways (Sandvig et al., 1989; Shogomori and Futerman, 2001; Abrami et al., 2003). Raft association cannot therefore be considered a criterion in and of itself for internalization via the caveolae/raft-dependent pathway.. Two caveolar ligands, SV40 and CTX, are delivered to a caveolin-1-positive endocytic compartment or caveosome (Parton et al., 1994; ...
The aminoglycoside antibiotic gentamicin is an ototoxic drug and has been used experimentally to investigate cochlear damage induced by noise. We have investigated the changes in the protein profile associated with caveolae in gentamicin treated and untreated spiral ligament (SL) pericytes, specialized cells in the blood labyrinth barrier of the inner ear microvasculature. Pericytes from various microvascular beds express caveolae, protein and cholesterol rich microdomains, which can undergo endocytosis and transcytosis to transport small molecules in and out the cells. A different protein profile in transport-specialized caveolae may induce pathological changes affecting the integrity of the blood labyrinth barrier and ultimately contributing to hearing loss. Caveolae isolation from treated and untreated cells is achieved through ultracentrifugation of the lysates in discontinuous gradients. Mass spectrometry (LC-MS/MS) analysis identifies the proteins in the two groups. Proteins segregating with
Purpose: : SPARC, an important matricellular protein in mammalian lens, was internalized by lens epithelium and cultured lens epithelial cells (LEC) in vitro. Caveolae-mediated endocytosis is an important endocytic pathway for the entry of macromolecules into cells. Caveolae have recently been implicated in organizing the cells signal transduction pathways and can play an important role in cell function. Caveolin-1 is the major coat protein of caveolae. Our goal is to characterize the expression of caveolin-1 in epithelial and fiber cells of the murine ocular lens and the cultured murine LEC, and to determine whether caveolae are involved in SPARC endocytosis. Methods: : Eyes from C57BL6/129SVJ wt and SPARC-null mice (1-4 month-old) were prepared for paraffin sections and frozen sections. LEC were cultured from wt and SPARC-null 1-2 month-old mice. The distribution of caveolin-1 in murine lens and cultured LEC was analyzed by immunohistochemistry and immunoblotting with antibodies against ...
The cell cytoskeleton plays a role in caveolar organization and trafficking. Actin stress fibers influence the linear distribution of caveolae at the plasma membrane in many cell types. Stress fibers regulated by the tyrosine kinase Abl and the formin mDIA1 play a major role in caveolar organization as well as endocytic trafficking initiated in response to loss of cell adhesion from the substrate [11]. The actin-binding protein Filamin A also plays a crucial role in trafficking of caveolae linked to actin [12]. Microtubules promote recycling of caveolae through local stabilization of microtubules by β1 integrins and integrin-linked kinase (ILK) signaling (reviewed in [13]). The β1 integrin-ILK recruits the actin-binding protein IQGAP1 which together with mDIA1 stabilize microtubules. Thus mDIA1 which regulates both actin and microtubules is crucial for both the internalization and recycling of caveolae.. Caveolae can flatten in response to membrane stretch and this mechanosensitive response of ...
Metastasis is a multistep process requiring cancer cell signaling, invasion, migration, survival, and proliferation. These processes require dynamic modulation of cell surface proteins by endocytosis. Given this functional connection, it has been suggested that endocytosis is dysregulated in cancer. To test this, we developed In-Cell ELISA assays to measure three different endocytic pathways: clathrin-mediated endocytosis, caveolae-mediated endocytosis, and clathrin-independent endocytosis and compared these activities using two different syngeneic models for normal and oncogene-transformed human lung epithelial cells. We found that all endocytic activities were reduced in the transformed versus normal counterparts. However, when we screened 29 independently isolated non-small cell lung cancer (NSCLC) cell lines to determine whether these changes were systematic, we observed significant heterogeneity. Nonetheless, using hierarchical clustering based on their combined endocytic properties, we ...
Abstract: Low molecular weight protein tyrosine phosphatases (LMW-PTPs) are small enzymes that are ubiquitous in many organisms. They are important in biological processes such as cell proliferation, adhesion, migration, and invasiveness. LMW-PTP is expressed in mammalian cells as two isoforms (IF1 and IF2) originating through alternative splicing. We have previously shown that IF2 targets lipid rafts called caveolae and interacts with caveolin-1, their major structural protein. Caveolae are cholesterol- and sphingolipid-rich membrane microdomains that have been implicated in a variety of cellular functions, including signal transduction events. Caveolin-1 contains a scaffolding region that contributes to the binding of the protein to the plasma membrane and mediates protein omo- and etero-oligomerization. Interaction of many signaling molecules with the scaffolding domain sequesters them into caveolae and inhibits or suppresses their activities. Caveolin-interacting proteins usually have a ...
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Representative traces and indicate data showing the response of shortening (expressed as a % of resting cell size) to selective stimulation of b1-AR with one hundred nM isoproterenol (Iso) in the existence of 100 nM ICI 118,551. C, D. Representative traces and suggest information of the response of [Ca2+]i transient amplitude to selective b1-AR stimulation. Knowledge are from 200 myocytes from 7 hearts. E, F, G. Agent traces and suggest information of the reaction of ICa,L to selective stimulation of b1-AR with 10 nM Iso in the presence of ICI 118, 551 (ICI). n = six myocytes from three hearts. P,.05, Students t-take a look at. doi:ten.1371/journal.pone.0106905.g005 In standard, the results of simvastatin on basal and b-AR stimulated grownup myocyte perform are quite comparable to individuals we have noted beforehand when caveolae are disrupted with MBCD, suggesting that simvastatin is operating by way of cholesterol-dependent (caveolar) pathways. Nonetheless, presented the human body of ...
Download and listen to every speaker at the FOCUS National Conference 2010 right to your computer.. Keynote:. Being Single - Matthew Kelly. Dont Impose Your Morality On Me - Dr. Edward Sri From First Date to Life Mate - Stenphanie Weinert From the Foundation of the World - Curtis Martin Msgr. Stuart Swetland, S.T.D. Testimony The Power of the Spiritual Life - Fr. Benedict Groeschel, CFR. Concurrent:. Can I Be Certain of the Existence of God - Sean Dalton Do I Really Need the Church - Sean Dalton Forgiving When You Dont Feel Like It - Jeff Cavins God Has a Plan of Sheer Goodness - Jeff Cavins Oprah or Jesus - Whose Plan will You Follow - Jeff Cavins Reaching the Lost - Peter Herbeck Who Do You Say That I Am - Debbie Herbeck Who Do You Say That I Am - Sean Dalton World Changers - Curtis Martin. Impact:. As The World Turns - Uncovering the Wounds of Those Born Since 1960 - Vicki Thorn Astrophysics and Creation - Fr. Robert Spitzer, S.J.. Building the Culture of Life - Lila Rose Casting Into the ...
Download and listen to every speaker at the FOCUS National Conference 2010 right to your computer.. Keynote:. Being Single - Matthew Kelly. Dont Impose Your Morality On Me - Dr. Edward Sri From First Date to Life Mate - Stenphanie Weinert From the Foundation of the World - Curtis Martin Msgr. Stuart Swetland, S.T.D. Testimony The Power of the Spiritual Life - Fr. Benedict Groeschel, CFR. Concurrent:. Can I Be Certain of the Existence of God - Sean Dalton Do I Really Need the Church - Sean Dalton Forgiving When You Dont Feel Like It - Jeff Cavins God Has a Plan of Sheer Goodness - Jeff Cavins Oprah or Jesus - Whose Plan will You Follow - Jeff Cavins Reaching the Lost - Peter Herbeck Who Do You Say That I Am - Debbie Herbeck Who Do You Say That I Am - Sean Dalton World Changers - Curtis Martin. Impact:. As The World Turns - Uncovering the Wounds of Those Born Since 1960 - Vicki Thorn Astrophysics and Creation - Fr. Robert Spitzer, S.J.. Building the Culture of Life - Lila Rose Casting Into the ...
Knowledge about the uptake mechanism and subsequent intracellular routing of non-viral gene delivery systems is important for the development of more efficient carriers. In this study we compared two
Respiratory syncytial virus (RSV) is an enveloped virus that assembles into filamentous virus particles on the surface of infected cells. Morphogenesis of RSV is dependent upon cholesterol-rich (lipid raft) membrane microdomains, but the specific role of individual raft molecules in RSV assembly is not well defined. Here we show that RSV morphogenesis occurs within caveolar membranes and that both caveolin-1 and cavin-1, the two major structural and functional components of caveolae, are actively recruited to and incorporated into the RSV envelope. The recruitment of caveolae occurred just prior to the initiation of RSV filament assembly, and was dependent upon an intact actin network as well as a direct physical interaction between caveolin-1 and the viral G protein. Moreover, cavin-1 protein levels were significantly increased in RSV-infected cells, leading to a virus-induced change in the stoichiometry and biophysical properties of the caveolar coat complex. Our data indicate that RSV ...
Altered fluid flow, which is found in branches and curvatures of arteries, results in abnormal forces on the endothelial cells (EC). These forces have been shown to alter EC gene expression and phenotype and to activate several cellular structures including G-proteins, ion channels, adhesion molecules, and caveolae. Recently, PECAM-1 has been implicated as the primary sensor of hemodynamic forces in EC. Shear stress rapidly induces tyrosine phosphorylation of PECAM-1 and the recruitment of SHP-2. These events appear to contribute to shear-activation of ERK1/2. Additionally, PECAM-1 has been shown to form a mechanosensory signaling complex with VE-cadherin, VEGFR2, and βcatenin which plays a role in adhesion molecule expression and regulation of NF-κB. Past work has shown that caveolae membrane domains also serve as mechanotransduction sites that regulate many of these same second messengers. Based on these novel observations, we hypothesize that the PECAM-1 mediated mechanotransduction ...
Several exogenous and endogenous cargo proteins are internalized independently of clathrin, including the bacterial Shiga toxin. The mechanisms underlying early steps of clathrin-independent uptake remain largely unknown. In this study, we have designed a protocol to obtain gradient fractions containing Shiga toxin internalization intermediates. Using stable isotope labeling with amino acids in cell culture (SILAC) and quantitative mass spectrometry, Rab12 was found in association with these very early uptake carriers. The localization of the GTPase on Shiga toxin-induced plasma membrane invaginations was shown by fluorescence microscopy in cells transfected with GFP-Rab12. Furthermore, using a quantitative biochemical assay, it was found that the amount of receptor-binding B-subunit of Shiga toxin reaching the trans-Golgi/TGN membranes was decreased in Rab12-depleted cells, and that cells were partially protected against intoxication by Shiga-like toxin 1 under these conditions. These findings ...
Introduction: Caveolins (Cav), the principal structural proteins of the caveolar domain, have been implicated in the development of cardiac hypertrophy, pulmonary hypertension (PH) and lung remodeling. Mice with homozygous deletion of the Cav-1 gene display cardiac hypertrophy and pulmonary abnormalities characterized by thickened alveolar septa, hypercellularity and PH. In vivo administration of a cell-permeable Cav-1 mimetic peptide was previously shown to prevent the development of monocrotaline-induced pulmonary artery medial hypertrophy, PH and right ventricular hypertrophy in rats. Whether administration of such a Cav-1 peptide could rescue the cardio-pulmonary defects observed in Cav-1 KO mice remains unknown.. Methods and Results: Three week-old wild-type and Cav-1 KO mice were randomly assigned to receive a daily intraperitoneal injection of either saline, penetratin alone (AP, 2.5 mg/kg/d) or a peptide consisting of the Cav-1 scaffolding domain coupled to penetratin (AP-Cav, 2.5 ...
article{744458, author = {Remaut, Katrien and Sanders, Niek and De Geest, Bruno and Braeckmans, Kevin and Demeester, Jo and De Smedt, Stefaan}, issn = {0927-796X}, journal = {MATERIALS SCIENCE \& ENGINEERING R-REPORTS}, keyword = {SINGLE-PARTICLE TRACKING,FLUORESCENCE CORRELATION SPECTROSCOPY,CROSS-CORRELATION SPECTROSCOPY,advanced light microscopy,nuclear uptake,endocytosis,extracellular matrix,non-viral carriers,gene therapy,GLYCOL-POLYETHYLENIMINE/DNA COMPLEXES,CYSTIC-FIBROSIS SPUTUM,BLOCK-COPOLYMER MICELLES,RESONANCE ENERGY-TRANSFER,CAVEOLAE-MEDIATED ENDOCYTOSIS,INTRAVENOUS GENE DELIVERY,IMAGE CORRELATION SPECTROSCOPY}, language = {eng}, pages = {117--161}, title = {Nucleic acid delivery: Where material sciences and bio-sciences meet}, url = {http://dx.doi.org/10.1016/j.mser.2007.06.001}, volume = {58}, year = {2007 ...
Virus tracking in living NMuMG epithelial cells expressing GFP-fused caveolin-1. (a) Caveolar endocytosis, fission and fusion events, and internal vesicle membrane dynamics are shown not to be affected in a stable cell line. (b) Detail of an infected cell (in a surface confocal section) with a virion bypassing a caveolin-rich domain. (c) In-depth confocal section with a virion(s) captured in a caveolin-rich vesicle at the nuclear periphery. (d and e) Uptake of virions through caveolin membrane domains at the cell surface. Mouse NMuMG epithelial cells expressing GFP-tagged caveolin-1 were infected with Alexa 594-prestained virus (MOI of 10e3 virus particles per cell) and scanned in an open, mediumcontaining chamber with a deltaT of 6 s. Bars, 5 um (a to c) and 2 um (d and e). Liebl et al., J Virol 2006. ...
The global obesity epidemic has caused an explosion in the prevalence of metabolic diseases such as type II diabetes and cardiovascular disease. Intensive efforts have focused on the identification of therapeutic targets to better prevent and treat metabolic syndrome. Previous work had identified functional roles of adipocyte and endothelial CAV1 in susceptibility to diet-induced obesity (Razani et al., 2002), insulin resistance (Cohen et al., 2003) and atherosclerosis (Frank et al., 2004). In this study, we expanded upon these findings by using zebrafish larvae to visualize the localization of enterocyte Cav1 and caveolar endocytosis, and a knockout mouse model to identify a role for CAV1 in the intestinal epithelia in the regulation of plasma FAs and LDL cholesterol, lipids that contribute to the development of several metabolic diseases.. Although it is known that CAV1 is expressed in the intestinal epithelium (Field et al., 1998), the intracellular localization of this protein has remained ...
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Mouse Monoclonal Anti-Caveolin-1 Antibody (7C8) [DyLight 488]. Caveolae Marker, Endosome Marker. Validated: WB, Flow, ICC/IF, IHC-Fr, IHC-P. Tested Reactivity: Human, Mouse, Rat, and more. 100% Guaranteed.
Rabbit Monoclonal Anti-Caveolin-1 Antibody (SP43). Caveolae Marker, Endosome Marker. Validated: IHC, IHC-P. Tested Reactivity: Human. 100% Guaranteed.
How does fresh arterial blood make its way through the vast web of tiny vessels in the brain to reach the hungriest neurons? Using live imaging in mice, two studies reveal new elements of neurovascular physiology that play a role. For one, arteriolar endothelial cells were covered with inlets called caveolae, which somehow dictated the dilation and contraction of the vessels. The other identified specialized sphincters that controlled the flow of blood from arterioles into downstream capillary beds.. ...
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Caveolin-3 is a protein that in humans is encoded by the CAV3 gene. Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites. This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), HyperCKemia, distal myopathy or rippling muscle disease (RMD). Other mutations in Caveolin causes Long QT Syndrome or familial hypertrophic cardiomyopathy, although the role of Cav3 in Long QT syndrome has recently been disputed. Caveolin ...
Malignant gliomas represent a difficult therapeutic challenge due to the invasive nature of the tumor and limited tumoral delivery of therapeutic agents. In this study, novel nano liposome carriers composed of sulfatides were developed for the glioma targeted delivery. Firstly, sulfatides-containing liposomes (SCLs) were found to interact with glioma cells specifically. The specific interactions between sulfatides and tenascin-c (TN-C), a glioma overexpressed protein, may have an important role. Secondly, the mechanism of intracellular delivery of SCLs was studied. SCLs were found to be effectively internalized in glioma cells by both clathrin-dependent and caveolae/lipids rafts pathways. Thirdly, doxorubicin (DOX) was effectively loaded into the SCLs to form a liposomal drug, SCL-DOX. SCL-DOX could effectively accumulate in the nuclei of glioma cells that resulted in superior in vitro cytotoxicity. In a subcutaneous xenografts animal model, SCL-DOX could effectively inhibit tumor growth and ...
Background Chemotherapeutic drugs and newly developed therapeutic monoclonal antibodies are adequately delivered to most solid and systemic tumors. However, drug delivery into primary brain tumors and metastases is impeded by the blood-brain tumor barrier (BTB), significantly limiting drug use in brain cancer treatment. Methodology/Principal Findings We examined the effect of phosphodiesterase 5 (PDE5) inhibitors in nude mice on drug delivery to intracranially implanted human lung and breast tumors as the most common primary tumors forming brain metastases, and studied underlying mechanisms of drug transport. In vitro assays demonstrated that PDE5 inhibitors enhanced the uptake of [14C]dextran and trastuzumab (Herceptin®, a humanized monoclonal antibody against HER2/neu) by cultured mouse brain endothelial cells (MBEC). The mechanism of drug delivery was examined using inhibitors for caveolae-mediated endocytosis, macropinocytosis and coated pit/clathrin endocytosis. Inhibitor analysis strongly
According to our results in chickens, the possible channel units of DHPRs and RyRs in a sebokeratinocyte are peripherally located. This spatial relationship seems to resemble the arrangement of the smooth muscle cell in which the sarcoplasmic proteins, calsequestrin and RyRs colocalize with DHPRs in numerous, peripherally located sites within the caveolar domains (Moore et al., 2004; Pucovsky and Bolton, 2006). Due to the native arrangement of the stratified epidermis in our study, the exact array of DHPRs on the plasma membrane could not be revealed. However, RyRs were located in the proximity of the plasma membrane in horizontally aligned clusters, indicating the possible sites where the two channels might interact via spatial proximity. In a single smooth muscle cell of the urinary bladder, DHPRs have been shown to occupy the plasmalemma in longitudinal stripes that overlap almost entirely with the corresponding stripes formed by labelled RyR proteins (Moore et al., 2004). The authors ...
TY - JOUR. T1 - MURC/cavin-4 is co-expressed with Caveolin-3 in rhabdomyosarcoma tumors and its silencing prevents myogenic differentiation in the human embryonal RD cell line. AU - Faggi, Fiorella. AU - Codenotti, Silvia. AU - Poliani, Pietro Luigi. AU - Cominelli, Manuela. AU - Chiarelli, Nicola. AU - Colombi, Marina. AU - Vezzoli, Marika. AU - Monti, Eugenio. AU - Bono, Federica. AU - Tulipano, Giovanni. AU - Fiorentini, Chiara. AU - Zanola, Alessandra. AU - Lo, Harriet P.. AU - Parton, Robert G.. AU - Keller, Charles. AU - Fanzani, Alessandro. PY - 2015/6/18. Y1 - 2015/6/18. N2 - The purpose of this study was to investigate whether MURC/cavin-4, a plasma membrane and Z-line associated protein exhibiting an overlapping distribution with Caveolin-3 (Cav-3) in heart and muscle tissues, may be expressed and play a role in rhabdomyosarcoma (RMS), an aggressive myogenic tumor affecting childhood. We found MURC/cavin-4 to be expressed, often concurrently with Cav-3, in mouse and human RMS, as ...
Nucleoside diphosphate kinase B (NME2) is ubiquitously expressed; it is an enzyme responsible for the synthesis of nucleoside triphosphates.. NME2 acts as a transcriptional activator, plays a role in T-cell (TCR) receptor activation and TCR-mediated calcium influx. It is also involved in caveolae formation.. ...
Shibata and colleagues have shown previously that the application of peptides derived from the Gαs sequence in inside-out macropatches enhanced isoproterenol-evoked sodium current (INa), attributable to an apparent increase in the number of functional channels.5,6⇓ In this issue of Circulation Research, Shibata and colleagues (Yarbrough et al8) report that the source of these new channels could be caveolae. Using antibodies directed against caveolin-3, the muscle-specific isoform of caveolin, these investigators were able to block the direct effect of isoproterenol on the Na+ channel (ie, the PKA-independent, Gαs membrane-delimited pathway). Importantly, this effect seems specific, given that antibodies directed against other caveolin isoforms did not affect isoproterenol activation of Na+ channel activity. Moreover, Yarbrough et al8 also documented, in cardiac myocytes, the apparent preferential enrichment into caveolar microdomains of Na+ channels and Gαs. Although these data led the ...
Angiogenic growth factor-induced endothelial cell migration is a key step towards tumor angiogenesis. When cells are migrating, caveolin-1, the principle protein component of caveolae, is excluded from the leading edge and polarized to the cell rear. The migration-stimulated caveolin rear translocation appears to play an important role in endothelial cell polarization and directional movement. In
The overall objective of the proposed project is to harness a newly discovered, active transendothelial transport pathway, the caveolae pumping system, to provi...
Anderson HA, Chen Y, Norkin LC. 1998. MHC class I molecules are enriched in caveolae but do not enter with simian virus 40.. J Gen Virol. 79 ( Pt 6):1469-77. ...
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www.MOLUNA.de Caveolins in Cancer Pathogenesis, Prevention and Therapy [4196990] - Caveolins play an important role in the pathogenesis of multiple cancers. This volume focuses mainly on the importance of Caveolin-1 in breast, prostate, lung, skin, colon, pancreatic and brain cancers. It also studies the role of Caveolin-3 in breast cancer.Caveolins are important structural proteins of Caveolae, small invaginations of the
This is the first report that two different DIG fractions can be isolated from myelin by TX-100 extraction with sodium phosphate buffer lacking EDTA/EGTA, at room temperature. Both of these fractions have characteristics of GSL/cholesterol-enriched membrane domains based on their TX-100 insolubility, high ratios of cholesterol and GalC to phospholipid, enrichment in the GPI-linked protein NCAM120, and buoyancy on a sucrose density gradient. However, B2 also contained the cytoskeletal proteins actin and tubulin, and the raft markers flotillin-1, caveolin and GM1, and thus may consist of caveolae or other types of GSL/cholesterol-enriched membrane domain associated with cytoskeletal proteins. It does not contain non-membrane-associated cytoskeleton, which would sediment faster to a much higher density.. This finding of a caveolin-containing type of membrane domain associated with cytoskeletal proteins in myelin is also novel. Both caveolae and non-caveolar membrane domains have been found to ...
This download caveolins was been, and received on the treatment by Sir Henry Irving in 1875. Harold was in 1876, The Cup in 1881( at the Lyceum), The Promise of May( at the Globe) in 1882, Becket in 1884, with The Foresters in 1892. The download is one from which I give, not right of secure single-player of the purchase and of of rendering for the analysis.
Reggie-1 and -2 proteins (flotillin-2 and -1 respectively) form their own type of non-caveolar membrane microdomains, which are involved in important cellular processes such as T-cell activation, phagocytosis and signalling mediated by the cellular prion protein and insulin; this is consistent with the notion that reggie microdomains promote protein assemblies and signalling. While it is generally known that membrane microdomains contain large multiprotein assemblies, the exact organization of reggie microdomains remains elusive. Using chemical cross-linking approaches, we have demonstrated that reggie complexes are composed of homo- and hetero-tetramers of reggie-1 and -2. Moreover, native reggie oligomers are indeed quite stable, since non-cross-linked tetramers are resistant to 8 M urea treatment. We also show that oligomerization requires the C-terminal but not the N-terminal halves of reggie-1 and -2. Using deletion constructs, we analysed the functional relevance of the three predicted ...
TY - JOUR. T1 - Scratching the surface. T2 - Actin and other roles for the C-terminal Eps15 homology domain protein, EHD2. AU - Simone, Laura C.. AU - Naslavsky, Naava. AU - Caplan, Steven H. PY - 2014/3/1. Y1 - 2014/3/1. N2 - The C-terminal Eps15 homology domaincontaining (EHD) proteins participate in multiple aspects of endocytic membrane trafficking. Of the four mammalian EHD proteins, EHD2 appears to be the most disparate, both in terms of sequence homology, and in subcellular localization/function. Since its initial description as a plasma membrane-associated protein, the precise function of EHD2 has remained enigmatic. Various reports have suggested roles for EHD2 at the plasma membrane, within the endocytic transport system, and even in the nucleus. For example, EHD2 facilitates membrane fusion/repair in muscle cells. Recently the focus has shifted to the role of EHD2 in regulating caveolae. Indeed, EHD2 is highly expressed in tissues rich in caveolae, including fat, muscle and blood ...
Caveolins act as scaffold proteins in multiprotein complexes and have been implicated in signaling by G protein-coupled receptors. Studies using knock-out mice suggest that beta(3)-adrenoceptor (beta(3)-AR) signaling is dependent on caveolin-1; however, it is not known whether caveolin-1 is associated with the beta(3)-AR or solely with downstream signaling proteins. We have addressed this question by examining the impact of membrane rafts and caveolin-1 on the differential signaling of mouse beta(3a)- and beta(3b)-AR isoforms that diverge at the distal C terminus. Only the beta(3b)-AR promotes pertussis toxin (PTX)-sensitive cAMP accumulation. When cells expressing the beta(3a)-AR were treated with filipin III to disrupt membrane rafts or transfected with caveolin-1 siRNA, the cyclic AMP response to the beta(3)-AR agonist CL316243 became PTX-sensitive, suggesting G alpha(i/o) coupling. The beta(3a)-AR C terminus, S (P-384) under bar PLNR (P-389) under bar DG (Y-392) under bar EGARP (P-398) under ...
We found that lipid vesicles are internalized by both clathrin-mediated and clathrin-independent endocytosis pathways which require an acidification step for liposomes destabilization and fusion with the endosomes. The fusion event possibly triggers a microtubule driven pathway which avoids classical sorting endosomes and favours an ER pathway. This is an alternate to the SV40 virus caveolar route using a pH dependent, direct plasma membrane-to-ER pathway to efficiently deliver extracellular encapsulated cargo to the ER. Whilst further studies are needed to dissect this novel pathway in more detail, the finding of a second major ER targeting route leads to the idea that the import of molecules from the outside cell into the ER could be as important for the cell function as its secretion processes ...
Story ideas from the October 2007 issue of the Journal of Lipid Research include the study of the health effects of a relatively recent diet called alternate-day fasting; how atorvastatin reduces cholesterol and fat in blood vessels; how nutrition affects the breakdown of fats; and a review of intriguing structures on the surface of fat cells called caveolae.
In the second lecture, the next steps in viral infection are described. Endocytosis of plasma membrane bound viruses can occur via a number of mechanisms including caveolar, clathrin, non-clathrin, or lipid raft mediated pathways. The internalized vi
Buy our Caveolin-2 peptide. Ab4929 is a blocking peptide and has been validated in BL. Abcam provides free protocols, tips and expert support for WB and a 12…
Obligatory intracellular, human ehrlichiosis agents Ehrlichia chaffeensis and Anaplasma phagocytophilum have remarkable abilities to parasitize first-line immune defensive cells, monocytes/macrophages and granulocytes, respectively. As newly discovered pathogens, the bacterial or host components, the signaling pathways required for their internalization and proliferation, and how these bacteria inhibit the microbicidal activities in host cells remain vastly unknown. In this study, we found that the entry of E. chaffeensis into THP-1 cells rapidly induces the following essential signaling events: protein cross-linking by transglutaminase, tyrosine phosphorylation, phospholipase C (PLC)-?2 activation, IP 3 production, and an increase in cytosolic free calcium levels. The entry and proliferation of these pathogens involve caveolae-mediated endocytosis and glycosylphosphatidylinositol-anchored proteins. Furthermore, caveolar marker protein caveolin-1, tyrosine-phosphorylated proteins, and PLC-?2 are ...
The large GTPase dynamin 2 (Dyn2) helps cells internalize material by clathrin-and caveolae-mediated endocytosis. Its role in other internalization mechanisms is controversial, however. Now, Mark McNiven and colleagues report that Dyn2 drives fluid-phase micropinocytosis (internalization of small amounts of extracellular fluid), one of several mechanisms that cells use to sample their external environment (see p. 4167). Inhibition of all four Dyn2 splice variants by anti-dynamin antibodies or RNAi, the authors report, reduces the uptake of the fluid markers dextran and horseradish peroxidase in serum-starved epithelial cells. Dyn2 inhibition has no effect, however, on the uptake of these markers in serum-or EGF-stimulated cells, which suggests that Dyn2 is required for fluid-phase micropinocytosis but not for stimulated fluid uptake via macropinocytosis. The authors also report that cells expressing dominant-negative mutants of two specific Dyn2 variants-Dyn2(ba) and Dyn2(bb)-endocytose less ...
Expression of caveolins in RMS tumours. Double immunostain showing that in skeletal muscle Cav-1 and Cav-3 mark satellite cells and the plasmalemma of myofibres
Objective: 1α,25-dihydroxyvitamin D3 (1,25D3) regulates cells via two different mechanisms: VDR-dependent gene transcription and rapid membrane-signaling. In membrane-signaling, phospholipase-A2 (PLA2) is activated after 1,25D3 binds its membrane-associated receptor, protein disulfide isomerase family A, member 3 (Pdia3), leading to release of arachidonic acid, activation of phospholipase C (PLC), and activation of PLC-dependent protein kinase C (PKC). Caveolae are required for 1,25D3-dependent PKC activation. PLA2 activating protein (PLAA), which activates PLA2, exhibits homology with the G-protein beta subunit, and was considered to play a role in this process. The aim of the present study was to evaluate if and how PLAA mediates the membrane effects of 1,25D3. Method: Subconfluent cultures of MC3T3-E1 cells immunostained against PLAA, Cav-1 and Pdia3 were imaged using confocal microscopy. Wild type and PLAA-silenced (shPLAA) MC3T3-E1 osteoblasts were treated with either vehicle or 1,25D3; ...
Several new techniques are being used to find markers that could aid the development of targeting moieties with increased tumor specificity over those currently available. Luminal endothelial cell plasma membranes have been physically isolated from various normal tissues and lungs bearing nodules derived from a mammary adenocarcinoma cell line. A caveolar protein that was up-regulated specifically in the tumor endothelium was identified, used to generate a monoclonal antibody, and the antibody shown to accumulate specifically in the vasculature of a tumor (103) . Serial analysis of gene expression is also being used to identify tumor endothelial markers (104 , 105) . In the latter work, endothelial cells from dispersed human normal and malignant colorectal tissue were enriched and purified using endothelial cell markers. Serial analysis of gene expression libraries were generated and compared with libraries from other tissues. Of ,170 transcripts expressed predominantly in the endothelium, 79 ...
Lipids are the energy source used during liver regeneration. The research group, led by Dr. Albert Pol and with members from IDIBAPS, Universitat de Barcelona and Queensland University, unveils the essential role of the protein caveolin-1 in a fundamental process for liver cure after injury or transplant. Results also evidence the existence of cellular mechanisms by which excessive accumulation of lipids in the liver is a risk factor for the apparition of hepatic tumours.
1.The upper part of thalamus may be disc shaped, cup-shaped or flask-shaped. 2.Calyx, corolla and androecium arise from around the ovary and not beneath it. 3.Ovary is half-superior/half-inferior. 4.The ovary is placed at the bottom of cup or flask-shaped thalamus. Ovary wall is not fused with the thalamus. 5.Calyx, corolla and androecium often develop from …. Read more ...
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Cavins, a public school counselor and private therapist, says he took up vaping in 2014 to try to wean himself off chewing tobacco. While working at his computer on April 15 this year with his electronic smoke beside him, it "suddenly exploded, striking Joseph in the left eye, continuing past his head, hitting the ceiling, ricocheting off the wall and landing on top of the computer station, where it started a fire," he says in his May 19 complaint in Orange County Court ...
Cell-surface viral proteins most frequently enter the cell through clathrin or caveolae endocytosis. Respiratory syncytial virus antigen internalization by immune cells is via caveolin, however, uptake of paramyxovirus cell membrane proteins by non-immune cells is done through clathrin-coated pits. In this work, the uptake of respiratory syncytial virus cell surface glycoproteins by non-immune human epithelial cells was investigated through indirect immunofluorescence with polyclonal anti-RSV antibody and confocal lasser-scanner microscopy. Clathrin and caveolae internalization pathways were monitored through specific inhibitors monodansylcadaverine (MDC) and methyl-beta-cyclodextrin (MBCD), respectively. Internalization of RSV antigens was inhibited by MDC but not by MBCD, implying that clathrin-mediated endocytosis is the major uptake route of RSV antigens by an epithelial human cell line.
A pacing system delivers cardiac protection pacing to protect the heart from injuries associated with ischemic events. The pacing system detects an ischemic event and, in response, initiates one or more cardiac protection pacing sequences each including alternative pacing and non-pacing periods. In one embodiment, the pacing system initiates cardiac protection pacing sequences including at least one postconditioning sequence to protect the heart from a detected ischemic event and a plurality prophylactic preconditioning sequences to protect the heart from probable future ischemic events.
A pacing system delivers cardiac protection pacing to protect the heart from injuries associated with ischemic events. The pacing system detects an ischemic event and, in response, initiates one or more cardiac protection pacing sequences each including alternative pacing and non-pacing periods. In one embodiment, the pacing system initiates a cardiac protection pacing sequence in response to the detection of the onset of an ischemic event, such that a pacing concurrent conditioning therapy is applied during the detected ischemic event.
The molecular composition of plasma membranes is constantly remodeled by endocytosis and exocytosis. Eisosomes are large cytoplasmic protein assemblies that localize to specialized domains on the yeast plasma membrane. They are of uniform size and immobile, and their disruption leads to large aberrant plasma membrane invaginations and endocytic defects. It is unknown how eisosomes are formed or inherited and what governs their size, distribution, and location. Here we show that eisosomes are formed de novo in the bud of dividing cells. They colonize newly formed membrane at a fixed density in a polarized wave proceeding from the bud neck to the bud tip and become anchored at the site of their formation. Pil1, one of the two main eisosome subunits, emerges as the central regulator of eisosome biogenesis that determines both size and location of eisosomes. Lowering Pil1 expression leads to normal-sized eisosomes at a reduced density, suggesting that eisosomes must be of a minimal size. Conversely, ...
where to get vector overexpressing caveolin-1? - posted in Molecular Cloning: Trying to overexpress caveolin-1 in human cancer cells...are there readymade products out there which already has cav-1 attached to a dependable promoter? If so, where should I look? Thanks in advance!
Lipid rafts, microdomain heterogeneity and inter-organelle contacts: impacts on membrane preparation for proteomic studies.: In recent years, there has been con
Kallikreins are secreted proteases that may play a functional role and/or serve as a serum biomarker for the presence or progression of certain types of cancers. Kallikrein 6 (KLK6) has been shown to be upregulated in several types of cancers, including colon. The aims of this study were to elucidate pathways that influence KLK6 gene expression and KLK6 protein secretion in the HCT116 human colon cancer cells. Our data indicate a central role for caveolin-1 (CAV-1), the main structural protein of caveolae, in both KLK6 gene expression and protein secretion. Sucrose gradient subcellular fractionation reveals that CAV-1 and KLK6 colocalize to lipid raft domains in the plasma membrane of HCT116 cells. Furthermore, we show that CAV-1, although it does not directly interact with the KLK6 molecule, enhances KLK6 secretion from the cells. Deactivation of CAV-1, through SRC-mediated phosphorylation, decreased KLK6 secretion. We also demonstrate that, in colon cancer cells, CAV-1 increased the amount of
The plasma membrane of eukaryotic cells contains nanodomains known as lipid rafts. Cholesterol depletion is a widely used technique for studying lipid rafts and their involvement in cellular processes. Cholesterol depletion has been reported to cause both increased and abolished T cell signaling. The abolished cell signaling upon cholesterol depletion is likely to be caused by substantial cell death as demonstrated by cell viability measurements. We have investigated how cholesterol depletion alters T cell activation by analyzing Jurkat T cells upon extraction of 10, 20, 30, 40 and 50% of total cholesterol using methyl β cyclodextrin (MBCD), a protocol in which cholesterol depletion does not have any adverse effect on cell viability.Upon cholesterol depletion peripheral actin polymerization and aggregation of the lipid raft marker GM1 in the plasma membrane is observed. The aggregation of GM1 upon cholesterol depletion is dependent on signaling protein Lck. The aggregated GM1 domains colocalize ...
At the European Molecular Biology Laboratory (EMBL) in Heidelberg, Professor Parton was mentored by Gareth Griffiths, where he honed his skills in electron microscopy while collaborating with other groups throughout the institute including Jean Gruenberg and Marino Zerial. He has maintained these collaborations to this day, despite the groups now being spread around the globe.. Collaboration has been central to his career. In a collaborative project with the laboratory of Kai Simons, he observed that the newly-discovered protein, VIP21 (later renamed caveolin-1), was an abundant marker protein of caveolae. Also at the EMBL Parton and Michael Way discovered a second member of the caveolin family, M-caveolin, now termed caveolin-3.. At The University of Queensland he collaborated with Professor John Hancock. Together they showed that caveolin mutants can act as dominant negative inhibitory mutants and that one of the mutants was a highly potent inhibitor of Ras signalling. Inhibition was specific ...
1989) Brain capillary 46,000 Dalton protein is cytoplasmic actin and is localized to endothelial plasma membrane. J. Cereb. Blood Flow Metab. 9, 675-680. 102. , Parkos, C. , et al. (2000) Tight junctions are membrane microdomains. J. Cell Sci. 113, 1771-1781. 103. , and Lisanti, M. P. (2001) The caveolin triad: caveolae biogenesis, cholesterol trafficking, and signal transduction. Cytokine Growth Factor Rev. 12, 41-51. 104. Madara, J. , and Kaoutzani, P. (1992) The movement of solutes and cells across tight junctions. 36. Stewart, P. , and Wiley, M. J. (1981) Developing nervous tissue induces formation of blood-brain barrier characteristics in invading endothelial cells: a study using quail-chick transplantation chimeras. Dev. Biol. 84, 183-192. 37. , and Risau, W. (1992) Expression of vascular endothelial growth-factor during embryonic angiogenesis and endothelial-cell differentiation. Development 114, 521-532. 38. , Escobedo, J. , and Williams, L. T. (1992) The fms-like tyrosine kinase, a ...
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While many Americans around the country rang in the Fourth of July at beaches and barbeques, parade accidents and fireworks mishaps caused some Independence Day celebrations to turn tragic.
TY - JOUR. T1 - Methyl-β-cyclodextrin prevents angiotensin II-induced tachyphylactic contractile responses in rat aorta. AU - Linder, A. Elizabeth. AU - Thakali, Keshari M.. AU - Thompson, Janice M.. AU - Watts, Stephanie W.. AU - Webb, R Clinton. AU - Leite, Romulo. PY - 2007/10/1. Y1 - 2007/10/1. N2 - Tachyphylaxis or desensitization is frequently observed following angiotensin II type I (AT1) receptor activation by angiotensin II. One of the possible mechanisms contributing to receptor desensitization involves receptor internalization. In addition to clathrin-coated pits/vesicles, caveolae, small invaginations in the plasma membrane rich in cholesterol, may also be involved in receptor internalization. After activation, AT1 receptor partially redistributes to lipid-enriched domains. We hypothesize that AT1 receptor internalization via caveolae contributes to the tachyphylactic response observed to angiotensin II. Endothelium-denuded rat aortic rings were exposed to increasing concentrations ...
This image shows the changes that occur in the lipid-storing fat body cells of Drosophila when the abundance of spectrin is reduced. Spectrin is a cytoskeletal protein that forms a lining underneath the plasma membrane of cells. One of the functions of spectrin is to promote the formation of specialized membrane domains by stabilizing membrane proteins in the correct location. By using confocal microscopy to capture images at different focal planes, I was able to begin to characterize the changes that occur in these cells. The green panels show the localization of a plasma membrane marker, Cd8-GFP. The red panels show the localization of a cytoplasmic protein, DS Red. Wild-type fat body cells have a highly invaginated plasma membrane (images on the left). These membrane invaginations envelop a class of small lipid droplets, shown as regions of negative staining. When cells express dsRNA against spectrin, the plasma membrane invaginations are strikingly reduced, as are the class of small cortical ...
The interaction between a T cell and an antigen-presenting cell (APC) can trigger a signaling response that leads to T cell activation. Prior studies have shown that ligation of the T cell receptor (TCR) triggers a signaling cascade that proceeds through the coalescence of TCR and various signaling molecules (e.g., the kinase Lck and adaptor protein LAT [linker for T cell activation]) into microdomains on the plasma membrane. In this study, we investigated another ligand-receptor interaction (CD58-CD2) that facilities T cell activation using a model system consisting of Jurkat T cells interacting with a planar lipid bilayer that mimics an APC. We show that the binding of CD58 to CD2, in the absence of TCR activation, also induces signaling through the actin-dependent coalescence of signaling molecules (including TCR-zeta chain, Lck, and LAT) into microdomains. When simultaneously activated, TCR and CD2 initially colocalize in small microdomains but then partition into separate zones; this ...
Ligand-induced endocytosis of receptor tyrosine kinases (RTKs) is a dynamic process governed by numerous protein-protein and protein-lipid interactions. This is a major mechanism of signal termination and is also frequently impaired in cancer. The Cbl family of ubiquitin ligases has been shown to play a key role in downregulation of RTKs, by directing their ligand-induced ubiquitination and subsequent lysosomal degradation. My thesis work has led to the identification of novel, ubiquitin-ligase independent, functions of Cbl in receptor endocytosis. We demonstrated that the adaptor protein CIN85 links Cbl with epidermal growth factor receptor (EGFR) internalization. The three SH3 domains of CIN85 interact with Cbl/Cbl-b in a phosphotyrosine dependent manner, whereas its proline-rich region constitutively binds endophilins, known regulators of plasma membrane invagination. The SH3 domains of CIN85 recognize an atypical proline-arginine (PxxxPR) motif present in Cbl and Cbl-b. Moreover, we showed ...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
Inflammatory breast cancer (IBC) is an aggressive, metastatic and highly angiogenic form of locally advanced breast cancer with a relatively poor three-year survival rate. Breast cancer invasion has been linked to proteolytic activity at the tumor cell surface. Here we explored a role for active cathepsin B on the cell surface in the invasiveness of IBC. We examined expression of the cysteine protease cathepsin B and the serine protease urokinase plasminogen activator (uPA), its receptor uPAR and caveolin-1 in two IBC cell lines: SUM149 and SUM190. We utilized a live cell proteolysis assay to localize in real time the degradation of type IV collagen by IBC cells. IBC patient biopsies were examined for expression of cathepsin B and caveolin-1. Both cell lines expressed comparable levels of cathepsin B and uPA. In contrast, levels of caveolin-1 and uPAR were greater in SUM149 cells. We observed that uPA, uPAR and enzymatically active cathepsin B were colocalized in caveolae fractions isolated from SUM149

Caveolae - WikipediaCaveolae - Wikipedia

Caveolae at the US National Library of Medicine Medical Subject Headings (MeSH) Lajoie, P. and I.R. Nabi, Lipid rafts, caveolae ... In biology, caveolae (Latin for "little caves"; singular, caveola), which are a special type of lipid raft, are small (50-100 ... Caveolae can also serve as mechanosensors in various cell types. In endothelial cells, caveolae are involved in flow sensation ... Caveolae are also involved in regulation of channels and in calcium signaling. Caveolae also participate in lipid regulation. ...
more infohttps://en.wikipedia.org/wiki/Caveolae

Membrane microdomains and caveolae.  - PubMed - NCBIMembrane microdomains and caveolae. - PubMed - NCBI

Membrane microdomains and caveolae.. Kurzchalia TV1, Parton RG.. Author information. 1. Max Planck Institute for Molecular Cell ... In addition, several recent papers have suggested that caveolae, which are considered to be a specific form of raft, and ... caveolins, the major membrane proteins of caveolae, are involved in the dynamic cholesterol-dependent regulation of specific ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/10449327?dopt=Abstract

Caveolae: where incoming and outgoing messengers meet | PNASCaveolae: where incoming and outgoing messengers meet | PNAS

Cell Surface Orifices of Caveolae and Localization of Caveolin to the Necks of Caveolae in Adipocytes ... Plasmalemmal caveolae were first identified as an endocytic compartment in endothelial cells, where they appear to move ... Cholesterol Depletion Disrupts Caveolae and Insulin Receptor Signaling for Metabolic Control via Insulin Receptor Substrate-1, ... This raises the possibility that a third function for caveolae is to process hormonal and mechanical signals for the cell. ...
more infohttps://www.pnas.org/content/90/23/10909?ijkey=a8805ce02f7c8dcc3b74d23ecf531d59d4b01fb5&keytype2=tf_ipsecsha

CaveolaCaveola

... in the neck of the caveolae. Mature caveolae might be assembled in the Golgi apparatus. Caveolae can flatten out into the cell ... Caveolae might exists as single pits or can form a cluster of caveolae with non-caveolar membrane between the pits. In many ... producing large chains of interconnected caveolae. Another structural feature of caveolae in certain endothelia is the presence ... Caveolae each comprise a caveolar bulb with a diameter of 60-80 nm, connected to an opening of fairly constant diameter. ...
more infohttps://www.uniprot.org/locations/SL-0035

Emerging themes in lipid rafts and caveolae.  - PubMed - NCBIEmerging themes in lipid rafts and caveolae. - PubMed - NCBI

Emerging themes in lipid rafts and caveolae.. Galbiati F1, Razani B, Lisanti MP. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/11525727?dopt=Abstract

Life Without Caveolae | Science SignalingLife Without Caveolae | Science Signaling

Caveolae are small, flask-shaped invaginations found in abundance on the plasma membrane of epithelial cells. They have been ... The generation of an organelle knockout in an intact organism should shed light on the physiological pathways in which caveolae ... Surprisingly, the mice are viable, but totally lack morphologically identifiable caveolae. They do have pulmonary and vascular ... R. G. Parton, Life without caveolae. Science 293, 2404-2405 (2001). [Full Text] ...
more infohttps://stke.sciencemag.org/content/2001/102/tw363

Digging into caveolae | ScienceDigging into caveolae | Science

Thank you for your interest in spreading the word about Science.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
more infohttps://science.sciencemag.org/content/269/5229/1398?ijkey=c437ac4498df71b011c397d2afa0dc35556e57b0&keytype2=tf_ipsecsha

Caveolae/raft-dependent endocytosis | JCBCaveolae/raft-dependent endocytosis | JCB

2 A). Caveolae are not observed in endothelial cells of caveolin-1 knock-out mice, although some smooth caveolae-like ... Endocytosis of caveolae/raft domains. The internalization of caveolae is facilitated by disruption of the actin cytoskeleton, ... Sorting in caveolae/raft-dependent endocytosis. Caveolae and rafts represent highly heterogeneous populations of functionally ... In this review, we propose that caveolae and rafts are internalized via a common pathway, caveolae/raft-dependent endocytosis, ...
more infohttp://jcb.rupress.org/content/161/4/673

Cavin3 MGI Mouse Gene Detail - MGI:1923422 - caveolae associated 3Cavin3 MGI Mouse Gene Detail - MGI:1923422 - caveolae associated 3

J:244017 Zhu B, et al., Cavin-3 (PRKCDBP) deficiency reduces the density of caveolae in smooth muscle. Cell Tissue Res. 2017 ...
more infohttp://www.informatics.jax.org/marker/MGI:1923422

Caveolae-Dependent and -Independent Uptake of Albumin in Cultured Rodent Pulmonary Endothelial CellsCaveolae-Dependent and -Independent Uptake of Albumin in Cultured Rodent Pulmonary Endothelial Cells

... mice was through caveolae-independent pathway(s) including clathrin-coated pits that resulted in endosomal accumulation of ... considerably less is known about caveolae-independent pathways. In this current study, we confirmed that cultured rat pulmonary ... Although a critical role for caveolae-mediated albumin transcytosis in pulmonary endothelium is well established, ...
more infohttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0081903

Dynamin-mediated Internalization of Caveolae | JCBDynamin-mediated Internalization of Caveolae | JCB

To determine the surface density of caveolae, we counted the number of ruthenium red-stained caveolae per cell that were within ... Quantitated inhibition of caveola-mediated endocytosis in anti-dynamin antibody-injected hepatocytes. Caveola-mediated ... but closely resembled caveolae (Fig. 5). Caveolae ("small caves") are specializations of the plasma membrane that are found in ... 1993) Caveolae and sorting in the trans-Golgi network of epithelial cells. EMBO (Eur Mol Biol Organ) J 12:1597-1605, pmid: ...
more infohttp://jcb.rupress.org/content/141/1/85?ijkey=b3b30001514f1ffc8ebdf12d3201fe74e2577469&keytype2=tf_ipsecsha

Frontiers | Caveolae, Caveolins, Cavins, and Endothelial Cell Function: New Insights | PhysiologyFrontiers | Caveolae, Caveolins, Cavins, and Endothelial Cell Function: New Insights | Physiology

Caveolins and the more recently discovered cavins are the major protein components of caveolae. When caveolae were discovered, ... Caveolins and the more recently discovered cavins are the major protein components of caveolae. When caveolae were discovered, ... The aim of this review is to focus primarily on molecular and cellular aspects of the role of caveolae, caveolins, and cavins ... The aim of this review is to focus primarily on molecular and cellular aspects of the role of caveolae, caveolins, and cavins ...
more infohttps://www.frontiersin.org/articles/10.3389/fphys.2011.00120/full

Caveola | definition of caveola by Medical dictionaryCaveola | definition of caveola by Medical dictionary

... caveola explanation free. What is caveola? Meaning of caveola medical term. What does caveola mean? ... Looking for online definition of caveola in the Medical Dictionary? ... caveola. Also found in: Dictionary, Financial, Encyclopedia.. Related to caveola: Caveolins. caveola. [ka″ve-o´lah] (pl. caveo´ ... caveo´lae [L.] one of the minute pits or incuppings of the cell membrane formed during pinocytosis.. caveola. pl. caveolae ...
more infohttp://medical-dictionary.thefreedictionary.com/caveola

Inhibition of Caveolae Contributes to Propofol Preconditioning-Suppressed Microvesicles Release and Cell Injury by Hypoxia...Inhibition of Caveolae Contributes to Propofol Preconditioning-Suppressed Microvesicles Release and Cell Injury by Hypoxia...

Interestingly, the caveolae inhibitor Mβ-CD strengthened the protective effect of propofol preconditioning. We further found ... But the connection between EMVs, H/R, and caveolae remains largely unclear. Here, we found that H/R significantly increased the ... Inhibition of Caveolae Contributes to Propofol Preconditioning-Suppressed Microvesicles Release and Cell Injury by Hypoxia- ... Caveolae are 50-100 nm cell surface plasma membrane invaginations involved in many pathophysiological processes. Recent ...
more infohttps://www.hindawi.com/journals/omcl/2017/3542149/abs/

Dystrophy-associated caveolin-3 mutations reveal that caveolae couple IL6/STAT3 signaling with mechanosensing in human muscle...Dystrophy-associated caveolin-3 mutations reveal that caveolae couple IL6/STAT3 signaling with mechanosensing in human muscle...

Here, the authors show that caveolae can regulate IL6/STAT3 signaling in muscle cells under stress, and that dystrophy related ... Caveolae are mechanosensors and mutations of their coat proteins are implicated in muscle disorders, but molecular mechanisms ... Caveolae, interconnected caveolae, and aberrant sized caveolae are indicated with black arrowheads, asterisks, and white ... Caveolae and interconnected caveolae are indicated with arrowheads and asterisks, respectively. c Quantification of the number ...
more infohttps://www.nature.com/articles/s41467-019-09405-5?error=cookies_not_supported&code=c673395b-caf5-4c03-b345-c0d7cf32e803

Regulation of assembly and cell surface dynamics of caveolaeRegulation of assembly and cell surface dynamics of caveolae

Previous work from our lab showed that EHD2 is a dimeric ATPase localised to the caveolae neck and confines caveolae to the ... An example of one such invagination observed in many cell types is "Caveola". Caveolae are bulb shaped invaginations of the ... Caveolin1 acts as the hallmark of caveolae, whereas caveolin3 and cavin4 are limited to muscle cells. Caveolae are appreciated ... This thesis aims to understand the assembly of caveolae and the molecular machineries involved in the regulation of caveolae ...
more infohttp://umu.diva-portal.org/smash/record.jsf?pid=diva2%3A929812

DIGITAL.CSIC: PC12 cells have caveolae that contain TrkADIGITAL.CSIC: PC12 cells have caveolae that contain TrkA

PC12 cells have caveolae that contain TrkA. Other Titles: Caveolae-disrupting drugs inhibit nerve growth factor-induced, but ... Caveolae are cholesterol-enriched, caveolin-containing plasma membrane microdomains involved in vesicular transport and signal ... Here we demonstrate the presence of caveolae in PC12 cells and their involvement in NGF signaling. Our results showed the ... These data together with the cofractionation of Shc, Ras, caveolin, and TrkA in the caveolae fraction supported a role for ...
more infohttps://digital.csic.es/handle/10261/66721

WikiGenes - CAV1 - caveolin 1, caveolae protein, 22kDaWikiGenes - CAV1 - caveolin 1, caveolae protein, 22kDa

Role of caveolae and caveolins in health and disease. Cohen, A.W., Hnasko, R., Schubert, W., Lisanti, M.P. Physiol. Rev. (2004) ... VEGF-induced down-regulation of caveolin-1 expression also resulted in the morphological loss of cell surface caveolae ... The phosphorylation of caveolin-2 on serines 23 and 36 modulates caveolin-1-dependent caveolae formation. Sowa, G., Pypaert, M ... Epithelial growth factor-induced phosphorylation of caveolin 1 at tyrosine 14 stimulates caveolae formation in epithelial cells ...
more infohttp://www.wikigenes.org/e/gene/e/857.html

caveolae and caveolin1 - Biology Forum | Biology-Online Dictionary, Blog & Forumcaveolae and caveolin1 - Biology Forum | Biology-Online Dictionary, Blog & Forum

1.caveolae which are a special type of lipid raft, are small (50-100 nanometer) invaginations of the plasma membrane in many ... I would like to ask you some questions about the caveolae and caveolin1. I didnt understand the paper that I read on that ... caveolae and caveolin1. Discussion of all aspects of cellular structure, physiology and communication. ... And Caveolin1? it is a protein integral of PM, that is localizated on caveolae? What is the mechanosensing and ...
more infohttps://www.biology-online.org/biology-forum/viewtopic.php?p=142206

Influence of cholesterol/caveolin-1/caveolae homeostasis on membrane properties and substrate adhesion characteristics of adult...Influence of cholesterol/caveolin-1/caveolae homeostasis on membrane properties and substrate adhesion characteristics of adult...

Effect on caveolae content. CAV-1 is the major protein component of caveolae and, in fact, formation and expression of caveolae ... Parton RG, Simons K. The multiple faces of caveolae. Nat Rev Mol Cell Biol. 2007;8:185-94.CrossRefPubMedGoogle Scholar ... such that cholesterol level is regulated by CAV-1/caveolae; and 2) modification of cellular cholesterol/CAV-1/caveolae ... Cholesterol and caveolae: structural and functional relationships. Biochim Biophys Acta. 2000;1529:210-22.CrossRefPubMedGoogle ...
more infohttps://link.springer.com/article/10.1186/s13287-018-0830-4

Guanosine Triphosphate Cyclohydrolase I Expression and Enzymatic Activity Are Present in Caveolae of Endothelial Cells |...Guanosine Triphosphate Cyclohydrolase I Expression and Enzymatic Activity Are Present in Caveolae of Endothelial Cells |...

Guanosine Triphosphate Cyclohydrolase I Expression and Enzymatic Activity Are Present in Caveolae of Endothelial Cells. Timothy ... Guanosine Triphosphate Cyclohydrolase I Expression and Enzymatic Activity Are Present in Caveolae of Endothelial Cells ... Guanosine Triphosphate Cyclohydrolase I Expression and Enzymatic Activity Are Present in Caveolae of Endothelial Cells ... Guanosine Triphosphate Cyclohydrolase I Expression and Enzymatic Activity Are Present in Caveolae of Endothelial Cells ...
more infohttp://hyper.ahajournals.org/content/early/2008/12/22/HYPERTENSIONAHA.108.115709

Lipid composition of caveolae and of surrounding plasma membrane in rat adipocytesLipid composition of caveolae and of surrounding plasma membrane in rat adipocytes

1. Caveolae in insulin signalling in human and rat adipocytes. Open this publication in new window or tab ,,Caveolae in insulin ... Caveolae seem necessary for fhe maintenance of metabolic signalling, like glucose uptake, and defects in caveolae may thus be ... Caveolae are invaginations of the plasma membrane, characterised by the structural protein caveolin. Caveolae and caveolin have ... None of them were detected in caveolae. As a first comprehensive and quantitative analysis of purified caveolae from primary ...
more infohttp://liu.diva-portal.org/smash/record.jsf?pid=diva2:559411

Caveolae provide a specialized membrane environment for respiratory syncytial virus assembly | Journal of Cell ScienceCaveolae provide a specialized membrane environment for respiratory syncytial virus assembly | Journal of Cell Science

Our data indicate that RSV exploits caveolae for its assembly, and we propose that the incorporation of caveolae into the virus ... The recruitment of caveolae occurred just prior to the initiation of RSV filament assembly, and was dependent upon an intact ... Caveolae provide a specialized membrane environment for respiratory syncytial virus assembly Message Subject (Your Name) has ... Caveolae provide a specialized membrane environment for respiratory syncytial virus assembly. Alexander Ludwig, Tra Huong ...
more infohttp://jcs.biologists.org/content/early/2017/02/01/jcs.198853

Cavin family proteins and the assembly of caveolae | Journal of Cell ScienceCavin family proteins and the assembly of caveolae | Journal of Cell Science

Caveolae and the cavin family of proteins. (A) Electron micrograph of caveolar bulbs (see caveolae marked by red dotted line) ... 1A). Caveolae generally display an uneven cellular distribution, and caveola-associated proteins have distinct tissue-specific ... The question, therefore, arises as to how the distinct protein and lipid species of caveolae cooperate to control caveola ... 2013). Caveolae as plasma membrane sensors, protectors and organizers. Nat. Rev. Mol. Cell Biol. 14, 98-112. doi:10.1038/ ...
more infohttp://jcs.biologists.org/content/128/7/1269.full

Cavin-3 (PRKCDBP) deficiency reduces the density of caveolae in smooth muscleCavin-3 (PRKCDBP) deficiency reduces the density of caveolae in smooth muscle

... Zhu, Baoyi LU ; Swärd, Karl LU ; Ekman, Mari LU ... Our studies revealed that the loss of cavin-3 resulted in ∼40% reduction of the caveolae protein cavin-1 in vascular and ... Our studies revealed that the loss of cavin-3 resulted in ∼40% reduction of the caveolae protein cavin-1 in vascular and ... Our studies revealed that the loss of cavin-3 resulted in ∼40% reduction of the caveolae protein cavin-1 in vascular and ...
more infohttps://lup.lub.lu.se/search/publication/364f9c3e-a101-48eb-bf5b-ea6e0c163acd
  • The caveola is a small (apparently) uncoated pit mostly found in the cell membrane of many highly differentiated mammalian cells, such as adipocytes, endothelial cells and muscle cells. (uniprot.org)
  • In many tissues, and particularly in adipocytes, multiple caveolae are arranged around a central vacuolar domain. (uniprot.org)
  • We have isolated caveolae from purified plasma membrane of primary rat adipocytes using ultrasonication to disrupt the membrane followed by density gradient ultracentrifugation. (diva-portal.org)
  • After isolation of caveolae and using electron microscopy on cell membranes, the insulin receptor was demonstrated to be localised in caveolae of human adipocytes. (diva-portal.org)
  • We also used biochemical and morphological methods to show that the glucose transporter GLUT4 was translocated to caveolae in response to insulin in rat adipocytes, indicating fhat the caveola is the locale for glucose uptake in adipocytes. (diva-portal.org)
  • Cavin1 has been shown to be the main regulator of caveola formation in multiple tissues, with the sole expression of Cavin1 sufficient for morphological caveola formation in cells lacking caveolae but abundant in Cav1. (wikipedia.org)
  • As a first comprehensive and quantitative analysis of purified caveolae from primary cells, our results provide a firm basis for the examination of caveolae formation using artificial membranes. (diva-portal.org)
  • Caveolae-like membranes and the amyloid precursor protein : a critical reassessment. (lancs.ac.uk)
  • caveolae-like membranes (CLMs) with similar characteristics can be isolated fmm brain tissue. (lancs.ac.uk)
  • These results indicate that, rather than being a genuine component of caveolae-like membranes, APP in this fraction arises from conlamination of the CLM fradion with distind vesicles of a similar density. (lancs.ac.uk)
  • When caveolae were discovered, their functional role was believed to be limited to transport across the endothelial cell barrier. (frontiersin.org)
  • Recent evidence has indicated EMVs and caveolae may have functional effects in cells undergoing H/R injury. (hindawi.com)
  • We show here that the Cav3 P28L and Cav3 R26Q myotubes are unable to assemble sufficient amounts of functional caveolae at the plasma membrane, leading to a loss of membrane tension buffering and membrane integrity under mechanical stress. (nature.com)
  • The absence of functional caveolae in mutant myotubes uncouples the regulation of IL6/STAT3 signaling with mechanical stress, which results in the constitutive hyperactivation of the IL6/STAT3 signaling pathway and the upregulation of several muscle-related genes. (nature.com)
  • Finally, the expression of WT Cav3 in mutant myotubes is sufficient to restore a functional pool of caveolae and to rescue the coupling of caveolae mechanosensing with IL6/STAT3 signaling. (nature.com)
  • Thus, a subpopulation of ERα is localized to endothelial cell caveolae where they are coupled to eNOS in a functional signaling module that may regulate the local calcium environment. (ahajournals.org)
  • The generation of an organelle knockout in an intact organism should shed light on the physiological pathways in which caveolae play a critical role. (sciencemag.org)
  • The caveolae- and raft-dependent pathways are therefore characterized by a common sensitivity to cholesterol depletion and inhibition of dynamin function. (rupress.org)
  • Caveolae are appreciated as important plasma membrane structures in maintaining cellular homeostasis of many cell types. (diva-portal.org)
  • We also observed that cavin3 interacts with caveolin1 in a cholesterol dependent manner and cavin3 may promote scission by acting as a positive regulator of caveolae dynamics, opposite to the cellular function of EHD2. (diva-portal.org)
  • Caveolae generally display an uneven cellular distribution, and caveola-associated proteins have distinct tissue-specific expression profiles suggestive of highly specialised functions. (biologists.org)
  • Mature caveolae might be assembled in the Golgi apparatus. (uniprot.org)
  • Surprisingly, the mice are viable, but totally lack morphologically identifiable caveolae. (sciencemag.org)
  • Caveolae were first morphologically described in the 1950s by early electron microscopists ( 31 , 52 ). (asm.org)
  • Interestingly, the caveolae inhibitor M β -CD strengthened the protective effect of propofol preconditioning. (hindawi.com)
  • Moreover, combined deepetching and immunogold techniques revealed the presence of the NGF receptor TrkA in the caveolae of PC12 ceils. (csic.es)
  • Taken together, our results demonstrate the presence of caveolae in PC12 ceils, which contain the high affinity NGF receptor TrkA, and the specific involvement of these cholesterol-enriched plasma membrane microdomains in the propagation of the NGF-induced signal. (csic.es)
  • Caveolae are also involved in regulation of channels and in calcium signaling. (wikipedia.org)
  • Our study reveals that under mechanical stress the regulation of mechanoprotection by caveolae is directly coupled with the regulation of IL6/STAT3 signaling in muscle cells and that this regulation is absent in Cav3-associated dystrophic patients. (nature.com)
  • We also showed the function of cavin3 in the regulation of caveolae dynamics at the plasma membrane. (diva-portal.org)
  • Caveolae are considered to be sites of uptake of materials into the cell, expulsion of materials from the cell, or addition or removal of cell (unit) membrane to or from the cell surface. (thefreedictionary.com)
  • These results show the importance of caveolae-mediated uptake for successful gene expression and have implications for the rational design of non-viral gene delivery systems. (springer.com)
  • Caveolin1 acts as the hallmark of caveolae, whereas caveolin3 and cavin4 are limited to muscle cells. (diva-portal.org)
  • Thus, we conclude that inhibition of caveolae contributes to propofol preconditioning-suppressed microvesicles release and cell injury by H/R. (hindawi.com)
  • Our data indicate that RSV exploits caveolae for its assembly, and we propose that the incorporation of caveolae into the virus contributes to defining the biological properties of the RSV envelope. (biologists.org)
  • Caveolae can also serve as mechanosensors in various cell types. (wikipedia.org)
  • This raises the possibility that a third function for caveolae is to process hormonal and mechanical signals for the cell. (pnas.org)
  • Caveolae can be used for entry to the cell by some pathogens and so they avoid degradation in lysosomes. (wikipedia.org)
  • Caveolae have a role in the cell signaling, too. (wikipedia.org)
  • In developing muscle fibres, multiple caveolae are connected by a single neck to the cell membrane, producing large chains of interconnected caveolae. (uniprot.org)
  • pl. caveolae [L.] one of the minute pits or incuppings of the cell membrane formed during pinocytosis. (thefreedictionary.com)
  • The stringent roles of cavin3 and EHD2 control the equilibrium between stably cell surface associated caveolae and the "kiss and run" type of caveolae, undergoing rounds of fission and fusion. (diva-portal.org)
  • Previous work from our lab showed that EHD2 is a dimeric ATPase localised to the caveolae neck and confines caveolae to the cell surface. (diva-portal.org)
  • Findings from this study will contribute to the understanding of how cholesterol/CAV-1/caveolae regulates aspects of the cell membrane important to cell adhesion, substrate sensing, and microenvironment interaction. (springer.com)
  • Cholesterol supplementation to MSCs increased membrane cholesterol, and resulted in decreased membrane fluidity and localization of elevated numbers of caveolae and CAV-1 to the cell membrane. (springer.com)
  • Another structural feature of caveolae in certain endothelia is the presence of a stomatal diaphragm, which consists of a central density and radial spikes, in the neck of the caveolae. (uniprot.org)