A species of gram-negative, aerobic bacteria that consist of slender vibroid cells.
Proteins isolated from the outer membrane of Gram-negative bacteria.
Porins are protein molecules that were originally found in the outer membrane of GRAM-NEGATIVE BACTERIA and that form multi-meric channels for the passive DIFFUSION of WATER; IONS; or other small molecules. Porins are present in bacterial CELL WALLS, as well as in plant, fungal, mammalian and other vertebrate CELL MEMBRANES and MITOCHONDRIAL MEMBRANES.
A genus of gram-negative, aerobic, rod- or vibroid-shaped or fusiform bacteria that commonly produce a stalk. They are found in fresh water and soil and divide by binary transverse fission.
The etiologic agent of CHOLERA.
Proteins found in any species of bacterium.
The genetic complement of a BACTERIA as represented in its DNA.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
The science that investigates the principles governing correct or reliable inference and deals with the canons and criteria of validity in thought and demonstration. This system of reasoning is applicable to any branch of knowledge or study. (Random House Unabridged Dictionary, 2d ed & Sippl, Computer Dictionary, 4th ed)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The degree of 3-dimensional shape similarity between proteins. It can be an indication of distant AMINO ACID SEQUENCE HOMOLOGY and used for rational DRUG DESIGN.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
The portion of an interactive computer program that issues messages to and receives commands from a user.
The process of pictorial communication, between human and computers, in which the computer input and output have the form of charts, drawings, or other appropriate pictorial representation.
An ATP-dependent protease found in prokaryotes, CHLOROPLASTS, and MITOCHONDRIA. It is a soluble multisubunit complex that plays a role in the degradation of many abnormal proteins.
The period of the CELL CYCLE preceding DNA REPLICATION in S PHASE. Subphases of G1 include "competence" (to respond to growth factors), G1a (entry into G1), G1b (progression), and G1c (assembly). Progression through the G1 subphases is effected by limiting growth factors, nutrients, or inhibitors.
A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.

Chromosome methylation and measurement of faithful, once and only once per cell cycle chromosome replication in Caulobacter crescentus. (1/444)

Caulobacter crescentus exhibits cell-type-specific control of chromosome replication and DNA methylation. Asymmetric cell division yields a replicating stalked cell and a nonreplicating swarmer cell. The motile swarmer cell must differentiate into a sessile stalked cell in order to replicate and execute asymmetric cell division. This program of cell division implies that chromosome replication initiates in the stalked cell only once per cell cycle. DNA methylation is restricted to the predivisional cell stage, and since DNA synthesis produces an unmethylated nascent strand, late DNA methylation also implies that DNA near the replication origin remains hemimethylated longer than DNA located further away. In this report, both assumptions are tested with an engineered Tn5-based transposon, Tn5Omega-MP. This allows a sensitive Southern blot assay that measures fully methylated, hemimethylated, and unmethylated DNA duplexes. Tn5Omega-MP was placed at 11 sites around the chromosome and it was clearly demonstrated that Tn5Omega-MP DNA near the replication origin remained hemimethylated longer than DNA located further away. One Tn5Omega-MP placed near the replication origin revealed small but detectable amounts of unmethylated duplex DNA in replicating stalked cells. Extra DNA synthesis produces a second unmethylated nascent strand. Therefore, measurement of unmethylated DNA is a critical test of the "once and only once per cell cycle" rule of chromosome replication in C. crescentus. Fewer than 1 in 1,000 stalked cells prematurely initiate a second round of chromosome replication. The implications for very precise negative control of chromosome replication are discussed with respect to the bacterial cell cycle.  (+info)

The CtrA response regulator mediates temporal control of gene expression during the Caulobacter cell cycle. (2/444)

In its role as a global response regulator, CtrA controls the transcription of a diverse group of genes at different times in the Caulobacter crescentus cell cycle. To understand the differential regulation of CtrA-controlled genes, we compared the expression of two of these genes, the fliQ flagellar gene and the ccrM DNA methyltransferase gene. Despite their similar promoter architecture, these genes are transcribed at different times in the cell cycle. PfliQ is activated earlier than PccrM. Phosphorylated CtrA (CtrA approximately P) bound to the CtrA recognition sequence in both promoters but had a 10- to 20-fold greater affinity for PfliQ. This difference in affinity correlates with temporal changes in the cellular levels of CtrA. Disrupting a unique inverted repeat element in PccrM significantly reduced promoter activity but not the timing of transcription initiation, suggesting that the inverted repeat does not play a major role in the temporal control of ccrM expression. Our data indicate that differences in the affinity of CtrA approximately P for PfliQ and PccrM regulate, in part, the temporal expression of these genes. However, the timing of fliQ transcription but not of ccrM transcription was altered in cells expressing a stable CtrA derivative, indicating that changes in CtrA approximately P levels alone cannot govern the cell cycle transcription of these genes. We propose that changes in the cellular concentration of CtrA approximately P and its interaction with accessory proteins influence the temporal expression of fliQ, ccrM, and other key cell cycle genes and ultimately the regulation of the cell cycle.  (+info)

Cell cycle-dependent polar localization of an essential bacterial histidine kinase that controls DNA replication and cell division. (3/444)

The master CtrA response regulator functions in Caulobacter to repress replication initiation in different phases of the cell cycle. Here, we identify an essential histidine kinase, CckA, that is responsible for CtrA activation by phosphorylation. Although CckA is present throughout the cell cycle, it moves to a cell pole in S phase, and upon cell division it disperses. Removal of the membrane-spanning region of CckA results in loss of polar localization and cell death. We propose that polar CckA functions to activate CtrA just after the initiation of DNA replication, thereby preventing premature reinitiations of chromosome replication. Thus, dynamic changes in cellular location of critical signal proteins provide a novel mechanism for the control of the prokaryote cell cycle.  (+info)

Identification and transcriptional control of the genes encoding the Caulobacter crescentus ClpXP protease. (4/444)

The region of the Caulobacter crescentus chromosome harboring the genes for the ClpXP protease was isolated and characterized. Comparison of the deduced amino acid sequences of the C. crescentus ClpP and ClpX proteins with those of their homologues from several gram-positive and gram-negative bacteria revealed stronger conservation for the ATPase regulatory subunit (ClpX) than for the peptidase subunit (ClpP). The C. crescentus clpX gene was shown by complementation analysis to be functional in Escherichia coli. However, clpX from E. coli was not able to substitute for the essential nature of the clpX gene in C. crescentus. The clpP and clpX genes are separated on the C. crescentus chromosome by an open reading frame pointing in the opposite direction from the clp genes, and transcription of clpP and clpX was found to be uncoupled. clpP is transcribed as a monocistronic unit with a promoter (PP1) located immediately upstream of the 5' end of the gene and a terminator structure following its 3' end. PP1 is under heat shock control and is induced upon entry of the cells into the stationary phase. At least three promoters for clpX (PX1, PX2, and PX3) were mapped in the clpP-clpX intergenic region. In contrast to PP1, the clpX promoters were found to be downregulated after heat shock but were also subject to growth phase control. In addition, the clpP and clpX promoters showed different activity patterns during the cell cycle. Together, these results demonstrate that the genes coding for the peptidase and the regulatory subunits of the ClpXP protease are under independent transcriptional control in C. crescentus. Determination of the numbers of ClpP and ClpX molecules per cell suggested that ClpX is the limiting component compared with ClpP.  (+info)

Cell cycle expression and transcriptional regulation of DNA topoisomerase IV genes in caulobacter. (5/444)

DNA replication and differentiation are closely coupled during the Caulobacter crescentus cell cycle. We have previously shown that DNA topoisomerase IV (topo IV), which is encoded by the parE and parC genes, is required for chromosomal partitioning, cell division, and differentiation in this bacterium (D. Ward and A. Newton, Mol. Microbiol. 26:897-910, 1997). We have examined the cell cycle regulation of parE and parC and report here that transcription of these topo IV genes is induced during the swarmer-to-stalked-cell transition when cells prepare for initiation of DNA synthesis. The regulation of parE and parC expression is not strictly coordinated, however. The rate of parE transcription increases ca. 20-fold during the G1-to-S-phase transition and in this respect, its pattern of regulation is similar to those of several other genes required for chromosome duplication. Transcription from the parC promoter, by contrast, is induced only two- to threefold during this cell cycle period. Steady-state ParE levels are also regulated, increasing ca. twofold from low levels in swarmer cells to a maximum immediately prior to cell division, while differences in ParC levels during the cell cycle could not be detected. These results suggest that topo IV activity may be regulated primarily through parE expression. The presumptive promoters of the topo IV genes display striking similarities to, as well as differences from, the consensus promoter recognized by the major Caulobacter sigma factor sigma73. We also present evidence that a conserved 8-mer sequence motif located in the spacers between the -10 and -35 elements of the parE and parC promoters is required for maximum levels of parE transcription, which raises the possibility that it may function as a positive regulatory element. The pattern of parE transcription and the parE and parC promoter architecture suggest that the topo IV genes belong to a specialized subset of cell cycle-regulated genes required for chromosome replication.  (+info)

Feedback control of a master bacterial cell-cycle regulator. (6/444)

The transcriptional regulator CtrA controls several key cell-cycle events in Caulobacter crescentus, including the initiation of DNA replication, DNA methylation, cell division, and flagellar biogenesis. CtrA is a member of the response regulator family of two component signal transduction systems. Caulobacter goes to great lengths to control the time and place of the activity of this critical regulatory factor during the cell cycle. These controls include temporally regulated transcription and phosphorylation and spatially restricted proteolysis. We report here that ctrA expression is under the control of two promoters: a promoter (P1) that is active only in the early predivisional cell and a stronger promoter (P2) that is active in the late predivisional cell. Both promoters exhibit CtrA-mediated feedback regulation: the early P1 promoter is negatively controlled by CtrA, and the late P2 promoter is under positive feedback control. The CtrA protein footprints conserved binding sites within the P1 and P2 promoters. We propose that the P1 promoter is activated after the initiation of DNA replication in the early predivisional cell. The ensuing accumulation of CtrA results in the activation of the P2 promoter and the repression of the P1 promoter late in the cell cycle. Thus, two transcriptional feedback loops coupled to cell cycle-regulated proteolysis and phosphorylation of the CtrA protein result in the pattern of CtrA activity required for the temporal and spatial control of multiple cell-cycle events.  (+info)

Regulation of podJ expression during the Caulobacter crescentus cell cycle. (7/444)

The polar organelle development gene, podJ, is expressed during the swarmer-to-stalked cell transition of the Caulobacter crescentus cell cycle. Mutants with insertions that inactivate the podJ gene are nonchemotactic, deficient in rosette formation, and resistant to polar bacteriophage, but they divide normally. In contrast, hyperexpression of podJ results in a lethal cell division defect. Nucleotide sequence analysis of the podJ promoter region revealed a binding site for the global response regulator, CtrA. Deletion of this site results in increased overall promoter activity, suggesting that CtrA is a negative regulator of the podJ promoter. Furthermore, synchronization studies have indicated that temporal regulation is not dependent on the presence of the CtrA binding site. Thus, although the level of podJ promoter activity is dependent on the CtrA binding site, the temporal control of podJ promoter expression is dependent on other factors.  (+info)

Bacterial cells: The migrating kinase and the master regulator. (8/444)

It is becoming clear that, as in eukaryotes, proteins in bacterial cells are targeted to specific cellular locations. The most recently discovered example is a remarkable histidine kinase that oscillates between polar and global distributions while temporally regulating transcription and DNA replication in Caulobacter.  (+info)

Caulobacter crescentus is an oligotrophic alpha-proteobacterium with a complex cell cycle involving sessile-stalked and piliated, flagellated swarmer cells. Because the natural lifestyle of C. crescentus intrinsically involves a surface-associated, sessile state, we investigated the dynamics and control of C. crescentus biofilms developing on glass surfaces in a hydrodynamic system. In contrast to biofilms of the well-studied Pseudomonas aeruginosa, Escherichia coli, and Vibrio cholerae, C. crescentus CB15 cells form biphasic biofilms, consisting predominantly of a cell monolayer biofilm and a biofilm containing densely packed, mushroom-shaped structures. Based on comparisons between the C. crescentus strain CB15 wild type and its holdfast (hfsA; DeltaCC0095), pili (DeltapilA-cpaF::Omegaaac3), motility (motA), flagellum (flgH) mutants, and a double mutant lacking holdfast and flagellum (hfsA; flgH), a model for biofilm formation in C. crescentus is proposed. For both biofilm forms, the holdfast ...
TY - JOUR. T1 - Use of transmissible plasmids as cloning vectors in Caulobacter crescentus. AU - Schoenlein, Patricia V. AU - Gallman, Lilly M.. AU - Ely, Bert. PY - 1988/10/30. Y1 - 1988/10/30. N2 - Cloning vectors for studies of Caulobacter crescentus genes should be transferable between Escherichia coli and C. crescentus since a transformation system has not been developed for C. crescentus. We have tested a large number of vectors containing IncP or IncQ replicons and found that many of the vectors containing IncQ replicons, and all but one of the vectors containing IncP replicons, are readily transferred by conjugation into C. crescentus. All of the plasmids tested were maintained in C. crescentus at 1 to 5 copies per cell, but plasmids containing IncP replicons were more stable than plasmids containing IncQ replicons. Further studies with a derivative of the IncQ plasmid R300B showed that when a promoterless kanamycin (Km)-resistance gene (npt2) was inserted into the intercistronic region ...
TY - JOUR. T1 - High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization. AU - Holden, Seamus J.. AU - Pengo, Thomas. AU - Meibom, Karin L.. AU - Fernandez, Carmen Fernandez. AU - Collier, Justine. AU - Manley, Suliana. N1 - Copyright: Copyright 2014 Elsevier B.V., All rights reserved.. PY - 2014/3/25. Y1 - 2014/3/25. N2 - We created a high-throughput modality of photoactivated localization microscopy (PALM) that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all stages of the cell cycle. We used high-throughput PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in live Caulobacter crescentus. We observed that FtsZ predominantly localizes as a patchy midcell band, and only rarely as a continuous ring, supporting a model of Z-ring organization whereby FtsZ protofilaments are randomly distributed within the band and interact only weakly. We found evidence for a previously ...
This chapter focuses on the aquatic bacterium Caulobacter crescentus, which divides asymmetrically during each cell division cycle, yielding progeny cells that differ both structurally and functionally. The initially motile swarmer cell progeny sheds its flagellum and differentiates into a nonmotile stalked cell. In addition to morphological differences, the stalked- and swarmer cell progeny inherit different competencies for chromosome replication. A central component of any cell cycle is the initiation of chromosome replication coupled with strict controls to prevent repeated rounds of DNA replication without intervening cell divisions. The Caulobacter origin of replication was identified and cloned by taking advantage of the observation that replication is always initiated in the stalked cell. Microbial cells are able to monitor changes in their environment, detect changes in cell density, and communicate with each other and with other organisms through signals. The Caulobacter DnaA protein is a
TY - JOUR. T1 - Flagellin redundancy in Caulobacter crescentus and its implications for flagellar filament assembly. AU - Faulds-Pain, Alexandra. AU - Birchall, Christopher. AU - Aldridge, Christine. AU - Smith, Wendy D.. AU - Grimaldi, Giulia. AU - Nakamura, Shuichi. AU - Miyata, Tomoko. AU - Gray, Joe. AU - Li, Guanglai. AU - Tang, Jay X.. AU - Namba, Keiichi. AU - Minamino, Tohru. AU - Aldridge, Phillip D.. PY - 2011/6. Y1 - 2011/6. N2 - Bacterial flagella play key roles in surface attachment and host-bacterial interactions as well as driving motility. Here, we have investigated the ability of Caulobacter crescentus to assemble its flagellar filament from six flagellins: FljJ, FljK, FljL, FljM, FljN, and FljO. Flagellin gene deletion combinations exhibited a range of phenotypes from no motility or impaired motility to full motility. Characterization of the mutant collection showed the following: (i) that there is no strict requirement for any one of the six flagellins to assemble a filament; ...
The requirement for transcription during development of the stalked bacterium, Caulobacter crescentus, was studied with synchronous cultures of swarmer cells. The developmental pattern in these bacteria was first established by determination of the times at which specific changes in cell structure and function occurred. These changes could be divided into those characteristic of (a) development of the swarmer cell into the stalked cell: loss of motility and synthesis of the stalk, and (b) development of the stalked cell into the asymmetric dividing cell: chromosome replication, synthesis of the flagellum, motility, and division. The effect of rifampin in blocking several of these steps-loss of motility, initiation of chromosome replication, and cell division-indicates that RNA synthesis is required throughout the cell cycle for normal differentiation.. ...
Periodic activation and deactivation of the essential transcriptional regulator CtrA is necessary to drive cell cycle progression in Caulobacter crescentus. At the onset of DNA replication (the G1-S cell cycle transition), CtrA and the AAA+ protease ClpXP colocalize at one cell pole along with three …
The structural maintenance of chromosomes (SMC) complex plays an important role in chromosome organization and segregation in most living organisms. In Caulobacter crescentus, SMC is required to align the left and the right arms of the chromosome that run in parallel down the long axis of the cell. However, the mechanism of SMC-mediated alignment of chromosomal arms remains elusive. Here, using genome-wide methods and microscopy of single cells, we show that Caulobacter SMC is recruited to the centromeric parS site and that SMC-mediated arm alignment depends on the chromosome-partitioning protein ParB. We provide evidence that SMC likely tethers the parS-proximal regions of the chromosomal arms together, promoting arm alignment. Furthermore, we show that highly transcribed genes near parS that are oriented against SMC translocation disrupt arm alignment, suggesting that head-on transcription interferes with SMC translocation. Our results demonstrate a tight interdependence of bacterial chromosome
The biochemical properties of the maltodextrin-hydrolyzing enzymes of cold-tolerant proteobacterium Caulobacter crescentus CB15 remain to be elucidated, although whose maltodextrin transport systems were well investigated. We cloned the putative glucoamylase of C. crescentus CB15 (CauloGA) gene. The CauloGA gene product that was expressed in E. coli was prone to forming inclusion bodies; however, most of the gene product was expressed in a soluble and active form when it was expressed as a fusion protein with Staphylococcus Protein A. The fusion protein was purified using an IgG Sepharose column and was identified as the active GA. The optimum temperature and pH for the activity of this GA toward maltotriose as a substrate were approximately 40°C and 5.0, respectively, and a differential scanning fluorimetry (DSF) analysis revealed that the melting temperature (Tm) of CauloGA was 42.9°C. The kinetic analyses with maltotriose and other maltodextrins as the substrates indicated that CauloGA has higher
The deaths of nearby relatives have a curious effect on the bacterium Caulobacter crescentus -- surviving cells lose their stickiness. Indiana University Bloomington biologists report in an upcoming issue of Molecular Microbiology that exposure to the extracellular DNA (eDNA) released by dying neighbors stops the sticky holdfasts of living Caulobacter from adhering to surfaces, preventing cells from joining bacterial biofilms. Less sticky cells are more likely to escape established colonies, out to where conditions may be better.
The S-layer of Caulobacter is a two-dimensional paracrystalline array on the cell surface composed of a single protein, RsaA. We have established conditions for preparation of stable, soluble protein and then efficient in vitro recrystallization of the purified protein. Efficient recrystallization and long range order could not be obtained with pure protein only, though it was apparent that calcium was required for crystallization. Recrystallization was obtained when lipid vesicles were provided, but only when the vesicles contained the specific species of Caulobacter smooth lipopolysaccharide (SLPS) that previous studies implicated as a requirement for attaching the S-layer to the cell surface. The specific type of phospholipids did not appear critical; phospholipids rather different from those present in Caulobacter membranes or archaebacterial tetraether lipids worked equally well. The source of LPS was critical; rough and smooth variants of Salmonella typhimurium LPS as well as the rough ...
Genome analysis identified a large number of genes that would enable utilization of dilute carbon sources and provides a comprehensive picture of the strategies used by C. crescentus for survival in nutrient-limiting conditions. Unlike E. coli and Vibrio cholerae, C. crescentus has no OmpF-type outer membrane porins that allow the passive diffusion of hydrophilic substrates across the outer membrane. However, it does possess 65 members of the family of TonB-dependent outer membrane channels that catalyze energy-dependent transport across the outer membrane. This is more than any other organism thus far characterized, with the next highest being 34 in Pseudomonas aeruginosa (32), and with no other sequenced proteobacteria possessing more than 10. C. crescentus has substantially fewer cytoplasmic membrane transporters relative to genome size than either E. coli or V. cholerae (33). Given C. crescentus low nutrient habitat, it is surprising that PTS or ATP-binding cassette domain transporters, ...
Virginia Tech scientists have developed a quantitative, mathematical model of DNA replication and cell division for the bacterium Caulobacter crescentus.
Urs Jenal is a Swiss Microbiologist and Professor at the Biozentrum University of Basel, Switzerland. Urs Jenal studied Experimental Biology at the Swiss Federal Institute of Technology (ETH) Zurich and received his PhD from there in 1991. Subsequently, he completed postdoctoral research at the ETH Zurich and at Stanford University, USA. Since 1996, Jenal has taught and conducted research at the Biozentrum University of Basel; first as an Assistant Professor and since 2002 as a Professor of Molecular Microbiology. The research of Urs Jenal explores the molecular basis of signal transduction controlling the growth, development and behavior of bacteria. Jenal received international acclaim through his discovery of a new pathway, which is based on a new cyclic di-nucleotide (c-di-GMP) and which coordinates the formation of microbial biofilms and contributes to the development of chronic bacterial infections. With the model bacterium Caulobacter crescentus, Jenal discovered that c-di-GMP controls ...
https://doi.org/10.1101/436394. Felletti M., Omnus D.J. and Jonas K.§ (2019). Regulation of the replication initiator DnaA in Caulobacter crescentus. BBA Gene Regulatory Mechanisms. 1862(7):697-705. [link]. Schramm F.D., Heinrich K., Thüring M., Bernhardt J. and Jonas K.§ (2017). An essential regulatory function of the DnaK chaperone dictates the decision between proliferation and maintenance in Caulobacter crescentus. PLOS Genetics. 13:e1007148. [link]. Heinrich K., Sobetzko P. and Jonas K.§ (2016). A Kinase-Phosphatase Switch Transduces Environmental Information into a Bacterial Cell Cycle Circuit. PLOS Genetics. 12: e1006522. [link]. Liu J., Francis L.I., Jonas K., Laub M.T. and Chien P. (2016). ClpAP is an auxiliary protease for DnaA degradation in Caulobacter crescentus. Molecular Microbiology. 102:1075-1085 [link]. Heinrich K. and Jonas K.§ (2016) Bakterielle Überlebensstrategien - Zellzykluskontrolle als Stressantwort. BIOspektrum. 22: 41-43 [link]. Leslie D.J.*, Heinen C.*, Schramm ...
The holdfast is important for the attachment of C. crescentus cells to surfaces; however, little is known about the regulation of holdfast synthesis and holdfast attachment genes during the cell cycle. Furthermore, the time of holdfast appearance at the pole of the cell is not known. In this paper, we investigate two aspects of holdfast synthesis: the transcription of a holdfast attachment gene, hfaA, and the appearance of the holdfast at the pole of the cell. We demonstrate that hfaA transcription is temporally controlled during the cell cycle, with maximal transcription in the swarmer pole of the predivisional cell. We show that the holdfast is not present in swarmer cells or at the swarmer poles of predivisional cells. Our results suggest that the holdfast appears during the differentiation of swarmer to stalked cells.. The hfaA gene possesses a ς54 promoter sequence at the requisite distance from the transcription start site (16). However, our results indicate that hfaA is not transcribed ...
The Caulobacter cell cycle regulatory system controls many modular subsystems that organize the progression of cell growth and reproduction. A control system constructed using biochemical and genetic logic circuitry organizes the timing of initiation of each of these subsystems. The central feature of the cell cycle regulation is a cyclical genetic circuit-a cell cycle engine-that is centered around the successive interactions of five master regulatory proteins: DnaA, GcrA, CtrA, SciP, and CcrM whose roles were worked out by the laboratories of Lucy Shapiro and Harley McAdams.[8][9][10] These five proteins directly control the timing of expression of over 200 genes. The five master regulatory proteins are synthesized and then eliminated from the cell one after the other over the course of the cell cycle. Several additional cell signaling pathways are also essential to the proper functioning of this cell cycle engine. The principal role of these signaling pathways is to ensure reliable production ...
Cryoelectron microscope tomography (cryoEM) and a fluorescence loss in photobleaching (FLIP) assay were used to characterize progression of the terminal stages of Caulobacter crescentus cell division. Tomographic cryoEM images of the cell division site show separate constrictive processes closing first the inner membrane (IM) and then the outer membrane (OM) in a manner distinctly different from that of septum-forming bacteria. FLIP experiments had previously shown cytoplasmic compartmentalization (when cytoplasmic proteins can no longer diffuse between the two nascent progeny cell compartments) occurring 18 min before daughter cell separation in a 135-min cell cycle so the two constrictive processes are separated in both time and space. In the very latest stages of both IM and OM constriction, short membrane tether structures are observed. The smallest observed pre-fission tethers were 60 nm in diameter for both the inner and outer membranes. Here, we also used FLIP experiments to show that ...
It is thought that S-layers protect cells from phages and large molecules. Using a combination of cellular electron cryo tomography (cryo-ET) with subtomogram averaging and X-ray crystallography we have determined the structure of the complete and intact S-layer lattice on Gram negative Caulobacter crescentus cells. We showed that the lattice formed in the crystals matches perfectly the lattice formed on cells as determined at 7.4 Å, directly on cells ...
I will discuss our recent work that combined single-molecule imaging, fluorescence-recovery-after-photobleaching and optical tweezers to determine how the flagellar motor senses mechanical signals. I will present data that provides evidence that the chemotactic output of a molecular switch within the motor is influenced by the response to perturbations in viscous loads. The switch changes the direction of motorrotation and the torque generated is anisotropic. Yet, Caulobacter Crescentus cells that carry a single rigid flagellum, unlike E. coli cells that carry many, swim with similar speeds in the forward and backward directions. The resulting paradox that arises due to the time-reversibility of Stokes flow can be resolved with a simple model that incorporates cell-precession during swimming. Our observations indicate that motor mechanics are similar between the two species belonging to different classes of bacteria, and suggest that motor-mechanosensing might also be conserved. I will conclude ...
The citrate cycle (TCA cycle, Krebs cycle) is an important aerobic pathway for the final steps of the oxidation of carbohydrates and fatty acids. The cycle starts with acetyl-CoA, the activated form of acetate, derived from glycolysis and pyruvate oxidation for carbohydrates and from beta oxidation of fatty acids. The two-carbon acetyl group in acetyl-CoA is transferred to the four-carbon compound of oxaloacetate to form the six-carbon compound of citrate. In a series of reactions two carbons in citrate are oxidized to CO2 and the reaction pathway supplies NADH for use in the oxidative phosphorylation and other metabolic processes. The pathway also supplies important precursor metabolites including 2-oxoglutarate. At the end of the cycle the remaining four-carbon part is transformed back to oxaloacetate. According to the genome sequence data, many organisms seem to lack genes for the full cycle [MD:M00009], but contain genes for specific segments [MD:M00010 M00011 ...
Nucleotide excision repair (NER) is a mechanism to recognize and repair bulky DNA damage caused by compounds, environmental carcinogens, and exposure to UV-light. In humans hereditary defects in the NER pathway are linked to at least three diseases: xeroderma pigmentosum (XP), Cockayne syndrome (CS), and trichothiodystrophy (TTD). The repair of damaged DNA involves at least 30 polypeptides within two different sub-pathways of NER known as transcription-coupled repair (TCR-NER) and global genome repair (GGR-NER). TCR refers to the expedited repair of lesions located in the actively transcribed strand of genes by RNA polymerase II (RNAP II). In GGR-NER the first step of damage recognition involves XPC-hHR23B complex together with XPE complex (in prokaryotes, uvrAB complex). The following steps of GGR-NER and TCR-NER are similar ...
The SWISS-MODEL Repository is a database of annotated 3D protein structure models generated by the SWISS-MODEL homology-modelling pipeline
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Second messengers control a wide range of important cellular functions in eukaryotes and prokaryotes. Here we show that cyclic di-GMP, a global bacterial second messenger, promotes cell cycle progression in Caulobacter crescentus by mediating the specific degradation of the replication initiation inhibitor CtrA. During the G1-to-S-phase transition, both CtrA and its cognate protease ClpXP dynamically localize to the old cell pole, where CtrA is rapidly degraded. Sequestration of CtrA to the cell pole depends on PopA, a newly identified cyclic di-GMP effector protein. PopA itself localizes to the cell pole and directs CtrA to this subcellular site via the direct interaction with a mediator protein, RcdA. We present evidence that c-di-GMP regulates CtrA degradation during the cell cycle by controlling the dynamic sequestration of the PopA recruitment factor to the cell pole. Furthermore, we show that cell cycle timing of CtrA degradation relies on converging pathways responsible for substrate and ...
My research interests include Bacterial Cell Cycle Regulation and Signal Transduction mechanisms.. 1. Bacterial Cell Cycle Regulation- Epigenetic mechanisms regulating various physiological activities in the prokaryotic cells are increasingly being appreciated. Methylation of specific bases of the DNA molecule by methyltransferases is the most common epigenetic modification observed in the bacterial cells. This modification of nucleotides adds another level of regulation at the transcription; furthermore, it has a fundamental role in the cell physiological processes such as DNA replication, DNA mismatch repair, and virulence mechanisms in many pathogens. Among the prokaryotic DNA methyltransferases, Dam expressed among the Gammaproteobacteria is the most intensively studied. In contrast to Dam, another DNA methyltransferase CcrM (Cell Cycle Regulated Methylase) has been described in the Alphaproteobacteria, where it plays an important role in the cell cycle regulation of Caulobacter crescentus ...
To see our full project description see initial project presentation: [[1]] The goal of our project is to clone the gene, mreB, from Caulobacter crescentus (stalked shaped cell) into Escherchia coli (rod shaped cell) to see if mreB will affect E. colis cell shape in some way. We expect to see the E. coli cells lyse or change from their normal rod shape. Normal E. coli cell shape [[2]] Normal C. crescentus cell shape [[3]] Lysed E. coli cells [[4]] Abnormal E. coli cells [[5]], [[6]] MreB is a rod-shape determining gene that appears as bands or spirals encircling the cell. It is known to associate with mreC and penicillin-binding proteins to catalyze precursors for the peptidoglycan cell wall and correctly position polar bacterial proteins. It is also homologous of actin in eukaryotes. ...
In Caulobacter crescentus, the temporal and spatial expression of late flagellar genes is regulated by the 54 transcriptional activator, FlbD. Genetic experiments have indicated that the trans-acting factor FliX regulates FlbD in response to the progression of flagellar assembly, repressing FlbD activity until an early flagellar basal body structure is assembled. Following assembly of this structure, FliX is thought to function as an activator of FlbD. Here we have investigated the mechanism of FliX-mediated regulation of FlbD activity. In vitro transcription experiments showed that purified FliX could function as a repressor of FlbD-activated transcription. Transcription activated by a gain-of-function mutant of FlbD (FlbD-1204) that is active in vivo in the absence of an early flagellar structure, was resistant to the repressive effects of FliX. DNA binding studies showed that FliX inhibited the interaction of wild-type FlbD with enhancer DNA but did not effect FlbD-catalysed ATPase ...
Caulobacter crescentus is an interesting type of aquatic bacteria that can survive in harsh environments such as fresh water, salt water, and soil. Nutrien | Microbiology
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1MB0: Crystallographic and Biochemical Studies of DivK Reveal Novel Features of an Essential Response Regulator in Caulobacter crescentus.
Kühn, Juliane and Briegel, Ariane and Mörschel, Erhard et al. (2010) Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus. EMBO Journal, 29 (2). pp. 327-339. ISSN 0261-4189. PMCID PMC2824468. https://resolver.caltech.edu/CaltechAUTHORS:20100218-083022805 ...
The study of chromosomal replication and cell division of bacteria has extended beyond Escherichia coli, and important insights have emerged recently from studies in other species, especially Bacillus subtilis and Caulobacter crescentus. Cell division is coordinated with other cell cycle events such as genomic DNA synthesis that leads to chromosomal replication and partition, increase of cell mass, and cell expansion by cell wall synthesis. This chapter reviews the information about predicted genes related to chromosomal replication, plasmid replication, and cell division in Helicobacter pylori, and a plausible replication machinery of the bacterium is discussed in light of the current understanding of bacterial organization and function of replication and cell division. The DnaA protein is essential for the initiation of chromosomal replication and is highly conserved among different bacteria. Clinical isolates of H. pylori have been reported to carry plasmids ranging in size from 1.5 to 40 kb. Three
By William Margolin | The division of one cell into two daughter cells is the crux of biological reproduction. But how do cells determine where along their dimensions division will occur? For bacteria, the best-studied species for basic biology, including cytokinesis, are the old standbys Escherichia coli, Bacillus subtilis, and Caulobacter crescentus, mainly because of their easy cultivation…
ChIP-Chip stands for Chromatin Immunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genome-wide binding sites of a DNA-binding protein. While the basic principle is the same for all species, there are some differences in handling cells. This protocol is developed and tested for E. coli. It should work the same way for other bacteria but that remains to be proven. Published protocols also exist for other bacterial species, including Bacillus subtilis, Caulobacter crescentus and Mycobacterium tuberculosis. Because E. coli can be grown to high cell densities relative to eukaryotes, it is possible to generate sufficient DNA to label without using a PCR-based method [1]. This method uses strand displacement primer extension with Klenow DNA polymerase and amplifies the DNA ~10-fold, while simultaneously incorporating dye-coupled nucleotide. Other ChIP-Chip protocols can be found here: ...
C. crescentus undergoes a developmental step during its division cycle from a motile cell unable to replicate its DNA to a nonmotile cell that is competent for chromosome replication. We use synchronized populations of motile G1-phase cells to study the events that occur during development. C. crescentus also divides asymmetrically, producing one motile and one nonmotile cell at each division. Proteins in the predivisional cell are segregated to opposite ends to mediate the differentiation of identity after division. We study the locations and movements of individual proteins using fluorescence microscopy.. Specific problems that we are investigating include the following: regulated degradation of a key transcription factor during the motile-sessile developmental step; the roles, locations, and interactions of enzymes that participate in cell wall biosynthesis; and the regulatory functions of protein phosphorylation on serine, threonine, or tyrosine residues. ...
Caulobacter is a single-celled organism with a filament-like tail called a flagellum. As it swims, its rounded cellular head rotates in one direction, while the tail rotates in the opposite direction. This creates torque, which helps explain the bacteriums nonlinear movement through a fluid. What Tang and his team found, however, is that Caulobacter also is influenced by Brownian motion, which is the zigzagging motion that occurs when immersed particles are buffeted by the actions of the molecules of the surrounding medium. What that means, in effect, is that Caulobacter is being pinballed by the water molecules surrounding it as it swims ...
Marques, R. C., Marques, M. do V., Menck, C. F. M., Martins-Pinheiro, M., Galhardo, R. S., & Italiani, V. C. S. (2003). Identification of DNA repair genes in the Caulocater crescentus genome through the selection of clones sensitive to genotoxic agents. In Resumos. São Paulo: Comissão de Pesquisa do ICB/USP ...
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Principal Investigator:NAGAI Kazuo, Project Period (FY):1993 - 1995, Research Category:Grant-in-Aid for General Scientific Research (B), Research Field:応用微生物学・応用生物化学
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Ebersbach, Gitte and Briegel, Ariane and Jensen, Grant J. et al. (2008) A Self-Associating Protein Critical for Chromosome Attachment, Division, and Polar Organization in Caulobacter. Cell, 134 (6). pp. 956-968. ISSN 0092-8674. PMCID PMC2614312. https://resolver.caltech.edu/CaltechAUTHORS:EBEc08 ...
The U.S. Global Change Research Program (USGCRP) was established by Presidential Initiative in 1989 and mandated by Congress in the Global Change Research Act (GCRA) of 1990 to
Urazy amputacyjne w obrębie ręki z towarzyszącym wielopoziomowym uszkodzeniem tkanek stanowią przeciwwskazanie do replantacji. Szczególnie trudną decyzją jest dyskwalifikacja z zabiegu dotycząca kciuka. Doświadczenie mikrochirurgiczne oraz umiejętności rekonstrukcyjne pozwalają na podjęcie próby ratowania części kończyn z założenia nienadających się do uratowania. Ma to szczególnie istotne znaczenie w aspekcie poprawy jakości życia pacjentów. W pracy przedstawiono przypadek 42-letniego mężczyzny, który doznał subtotalnej amputacji ze zniszczeniem szkieletu kostnego oraz tkanek miękkich na całej długości powierzchni dłoniowej i grzbietowej kciuka prawej ręki. Pomimo przeciwwskazań podjęto próbę ratowania uszkodzonych tkanek kciuka. Napływ krwi tętniczej został odtworzony poprzez zespolenie tętnicy głównej kciuka z dystalnym naczyniem żylnym i wytworzenie w taki sposób przetoki tętniczo-żylnej. Układ kostny tymczasowo odtworzono dzięki ustabilizowaniu
A pair of 3/4 round aluminium bench dogs with integral spring so that once positioned, they remain where placed. Exceptionally easy to add to any bench by just drilling with a 3/4 forstner bit a neat and upright hole where needed. Care however should be
Zapraszamy do podjęcia studiów na wyjątkowych w skali kraju kierunkach: zootechnika, bioinżynieria produkcji żywności, zwierzęta w rekreacji, edukacji i terapii
By herself, Bloodtip is not too much a threat. Its the waves of Bloodtip Swarmer you need to be careful about. Though the adds have only 5 hp, they deliver a poison debuff that stacks and can really hurt if theyre not dealt with ASAP.. Update: Now a rare elite due to the removal of The Ashweb Matriarch in patch 5.4. ...
Looking for online definition of Caulobacter crescentus in the Medical Dictionary? Caulobacter crescentus explanation free. What is Caulobacter crescentus? Meaning of Caulobacter crescentus medical term. What does Caulobacter crescentus mean?
Gene expression within cells is known to fluctuate stochastically in time. However, the origins of gene expression noise remain incompletely understood. The bacterial cell cycle has been suggested as one source, involving chromosome replication, exponential volume growth, and various other changes in cellular composition. Elucidating how these factors give rise to expression variations is important to models of cellular homeostasis, fidelity of signal transmission, and cell-fate decisions. Using single-cell time-lapse microscopy, we measured cellular growth as well as fluctuations in the expression rate of a fluorescent protein and its concentration. We found that, within the population, the mean expression rate doubles throughout the cell cycle with a characteristic cell cycle phase dependent shape which is different for slow and fast growth rates. At low growth rate, we find the mean expression rate was initially flat, and then rose approximately linearly by a factor two until the end of the cell
Generation and filtering of gene expression noise by the bacterial cell cycle. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
In fact, it was only in the late 1990s that biologists discovered bacteria even had a cytoskeleton. The cytoskeleton was first identified in the cells of eukaryotic organisms (those, such as plants and animals, whose cells have specialized organelles and a discrete nucleus). Bacteria are tiny, for one thing, and until the advent of advanced imaging technology, scientists could not get a good look inside. The species Goley studies, Caulobacter crescentus, is a mere 500 nanometers across, or about one one-hundredth the size of an average human cell.. Second, a cell wall encases most bacterial species, and scientists assumed this semi-rigid structure obviated the need for a cytoskeleton.. These assumptions turned out to be wrong. In 1998, structural biologist Jan Löwe, in the United Kingdom, demonstrated that a bacterial protein called FtsZ is an evolutionary counterpart of tubulin, a key protein component of the eukaryote cytoskeleton. The finding implied that the cytoskeleton was not a ...
ID CAUSK_1_PE109 STANDARD; PRT; 537 AA. AC CAUSK_1_PE109; B0T9X1; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Drug resistance transporter, EmrB/QacA subfamily;Flags: DE Precursor; (CAUSK_1.PE109). GN OrderedLocusNames=Caul_5403; OS CAULOBACTER SP. K31. OC Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; OC Caulobacteraceae; Caulobacter. OX NCBI_TaxID=366602; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS CAUSK_1.PE109. CC Caulobacter sp. K31 plasmid pCAUL02, complete sequence. CC (1809 nt) ; CC -!- ANNOTATIONS ORIGIN:B0T9X1_CAUSK CC -!- GENE_FAMILY: HOG000219979 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; B0T9X1; -. DR EMBL; CP000929; ABZ74520.1; -; Genomic_DNA. DR RefSeq; YP_001672179.1; NC_010333.1. DR STRING; B0T9X1; -. DR GeneID; 5897187; -. DR GenomeReviews; CP000929_GR; Caul_5403. DR KEGG; cak:Caul_5403; -. DR OMA; HAGNSAT; -. DR ...
The correct functioning of cells requires the orchestration of multiple cellular processes, many of which are inherently dynamical. The conditions under which these dynamical processes entrain each other remain unclear. Here we use synthetic biology to address this question in the case of concurrent cellular oscillations. Specifically, we study at the single-cell level the interaction between the cell division cycle and a robust synthetic gene oscillator in Escherichia coli. Our results suggest that cell division is able to partially entrain the synthetic oscillations under normal growth conditions, by driving the periodic replication of the genes involved in the oscillator. Coupling the synthetic oscillations back into the cell cycle via the expression of a key regulator of chromosome replication increases the synchronization between the two periodic processes. A simple computational model allows us to confirm this effect ...
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City of Hope, Duarte, CA, USA. Abstract: Background/Purpose Genetic/genomic cancer risk assessment (GCRA) identifies and prescribes personalized screening and prevention care for individuals and families with increased cancer risk, often prior to disease onset, when early detection or prevention strategies are most effective. GCRA is standard of care in most developed countries, but is not available in most of Latin America. Documented barriers include lack of affordable genomic tools, limited knowledge about GCRA among key stakeholders, and absence of clinicians trained for GCRA. City of Hope is leveraging the robust training resources of its Clinical Cancer Genetics Community of Practice (CCGCoP) to address the need for GCRA education and training in Latin America. Description The CCGCoP is a flexible, multi-modal program of distance didactics, face-to-face case-based workshops and continuing practice support. A program priority is training clinicians in underserved areas across the U.S. and ...
Complete information for PLEC gene (Protein Coding), Plectin, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Localization of CC2233 changes during the cell cycle.Swarmer cells were isolated from cultures of C. crescentus producing CC2233(1-62)-GFP and the cells were
Hardwick SW, Chan VS, Broadhurst RW, Luisi BF (March 2011). "An RNA degradosome assembly in Caulobacter crescentus". Nucleic ... crescentus and B. subtilis degradosomes respectively. The reason for the presence of these enzymes is currently unclear. This ...
nov., a novel predator of Caulobacter crescentus". International Journal of Systematic and Evolutionary Microbiology. 63 (1): ...
The bacterium Caulobacter crescentus contains a third protein, crescentin, that is related to the intermediate filaments of ... "Multiple large filament bundles observed in Caulobacter crescentus by electron cryotomography". Molecular Microbiology. 62 (1 ...
In Caulobacter crescentus Ccrm is produced at the end of the replication cycle when Ccrm recognition sites are hemimethylated, ... CcrM role have been characterized in the marine model organism Caulobacter crescentus, which is suitable for the study of cell ... Albu, R. F.; Jurkowski, T. P.; Jeltsch, A. (2012-02-01). "The Caulobacter crescentus DNA-(adenine-N6)-methyltransferase CcrM ... Woodcock, Clayton B.; Yakubov, Aziz B.; Reich, Norbert O. (August 2017). "Caulobacter crescentus Cell Cycle-Regulated DNA ...
... superresolution colocalization of intracellular protein superstructures and the cell surface in live Caulobacter crescentus". ...
"Two RND proteins involved in heavy metal efflux in Caulobacter crescentus belong to separate clusters within proteobacteria". ...
Laccases are also widely distributed among bacterial species, including Bacillus subtilis, Caulobacter crescentus, Escherichia ...
... concave side of the crescent-shaped bacterium Caulobacter crescentus. Both MreB and crescentin are necessary for C. crescentus ... MreB condenses from its normal helical network and forms a tight ring at the septum in Caulobacter crescentus right before cell ... Several rod shaped species, including Escherichia coli and Caulobacter crescentus, use one or more inhibitors of FtsZ assembly ... stalk formation by Caulobacter crescentus, and helical shape of Helicobacter pylori. Crenactin is an actin homologue unique to ...
Caulobacter crescentus, and began attempting to identify the specific biological processes controlling the cell's cycle. What ... The process of the Caulobacter cell cycle also show similarities to stem cell division, in which two distinct cells arise, one ... In each cell cycle, Caulobacter divides asymmetrically into two daughters. One, the swarmer cell, has a tail-like flagellum ... Examining the cell cycle control logic of Caulobacter as a state machine leads to understanding of bacterial cell cycle ...
Scientists have developed and explored a variety of scientific models, from Lucy Shapiro's single-celled Caulobacter crescentus ...
H7 and Caulobacter crescentus CB15. The sugar is also found in perimycin, an antibiotic produced by the Gram-positive organism ...
Regulatory Response to Carbon Starvation in Caulobacter crescentus. PLoS One. Landt S.G., Abeliuk E., McGrath P.T., Lesley J.A ... McAdams H.H., Shapiro L. (2008) Small non-coding RNAs in Caulobacter crescentus. Journal of Molecular Microbiology. Srinivasan ... Goley E.D., Yeh Y.C., Hong S.H., Fero M.J., Abeliuk E., McAdams H.H., and Shapiro L. (2011) Assembly of the Caulobacter cell ... The global regulatory architecture of transcription during the Caulobacter cell cycle., PLoS Genet. Abeliuk E, Christen B, Fero ...
Caulobacter crescentus, and Vibrio alginolyticus, the filament is made up of 11 protofilaments approximately parallel to the ...
With the model bacterium Caulobacter crescentus, Jenal discovered that c-di-GMP controls the transition from motile bacteria to ...
Caulobacter MeSH B03.440.400.425.288.100.100 - Caulobacter crescentus MeSH B03.440.400.425.290 - Cellvibrio MeSH B03.440. ... Caulobacter MeSH B03.660.050.090.100.100 - Caulobacter crescentus MeSH B03.660.050.340 - Holosporaceae MeSH B03.660.050.350 - ... Caulobacter MeSH B03.440.400.280.280 - Caulobacter crescentus MeSH B03.440.400.400 - gallionellaceae MeSH B03.440.400.425 - ...
C. crescentus is synonymous with Caulobacter vibrioides. The Caulobacter CB15 genome has 4,016,942 base pairs in a single ... Caulobacter crescentus is a member of a group of bacteria that possess the stalk structure, a tubular extension from the cell ... Caulobacter crescentus is a Gram-negative, oligotrophic bacterium widely distributed in fresh water lakes and streams. The ... Presumably, It does so by a gain of function after protein expansion from around 400 amino acids in Caulobacter crescentus to ...
In the rod-shaped bacteria E. coli, Caulobacter crescentus and B. subtilis cell size is controlled by a simple mechanisms in ...
Pilt näitab kolmemõõtmelist superresolutsiooniga kujutist Caulobacter crescentus'e bakteriraku pinnast (hall) ja märgistatud ...
The evolution of stalk positioning in the Caulobacter cladeEdit. Diverse positioning of the stalks. Caulobacter crescentus ( ... Caulobacter crescentus is a member of a group of bacteria that possess the stalk structure, a tubular extension from the cell ... Caulobacter crescentus is a Gram-negative, oligotrophic bacterium widely distributed in fresh water lakes and streams. The ... Jenal, U (Nov 2009). "The role of proteolysis in the Caulobacter crescentus cell cycle and development". Research in ...
Caulobacter crescentus on bakter, mis jaguneb kaheks erinevaks tütarrakuks. Seda kasutatakse rakujagunemise uurimiseks.[7] ...
In the rod-shaped bacteria E. coli, Caulobacter crescentus and B. subtilis cell size is controlled by a simple mechanisms in ...
Caulobacter crescentus on bakter, mis jaguneb kaheks erinevaks tütarrakuks. Seda kasutatakse rakujagunemise uurimiseks.[7] ...
C. crescentus is synonymous with Caulobacter vibrioides. The Caulobacter CB15 genome has 4,016,942 base pairs in a single ... Caulobacter crescentus is a member of a group of bacteria that possess the stalk structure, a tubular extension from the cell ... Caulobacter crescentus is a Gram-negative, oligotrophic bacterium widely distributed in fresh water lakes and streams. The ... Presumably, It does so by a gain of function after protein expansion from around 400 amino acids in Caulobacter crescentus to ...
Complete genome sequence of Caulobacter crescentus. William C. Nierman, Tamara V. Feldblyum, Michael T. Laub, Ian T. Paulsen, ... Complete genome sequence of Caulobacter crescentus. William C. Nierman, Tamara V. Feldblyum, Michael T. Laub, Ian T. Paulsen, ... Complete genome sequence of Caulobacter crescentus. William C. Nierman, Tamara V. Feldblyum, Michael T. Laub, Ian T. Paulsen, ... The complete genome sequence of Caulobacter crescentus was determined to be 4,016,942 base pairs in a single circular ...
The evolution of stalk positioning in the Caulobacter cladeEdit. Diverse positioning of the stalks. Caulobacter crescentus ( ... Caulobacter crescentus is a member of a group of bacteria that possess the stalk structure, a tubular extension from the cell ... Caulobacter crescentus is a Gram-negative, oligotrophic bacterium widely distributed in fresh water lakes and streams. The ... Jenal, U (Nov 2009). "The role of proteolysis in the Caulobacter crescentus cell cycle and development". Research in ...
1984) Caulobacter crescentus flagellar filament has a right-handed helical form. J Mol Biol 173(1):125-130. ... Helical motion of the cell body enhances Caulobacter crescentus motility. Bin Liu, Marco Gulino, Michael Morse, Jay X. Tang, ... Helical motion of the cell body enhances Caulobacter crescentus motility Message Subject (Your Name) has sent you a message ... 2006) Low flagellar motor torque and high swimming efficiency of Caulobacter crescentus swarmer cells. Biophys J 91(7):2726- ...
Walker S.G., Smit J. (1993) Attachment of the S-Layer of Caulobacter crescentus to the Cell Surface. In: Beveridge T.J., Koval ... Smit, J., Grano, D.A., Glaser, R.M., and Agabian, N., 1981, Periodic surface array in Caulobacter crescentus: fine structure ... Gilchrist, A., Fisher, J.A., and Smit, J., 1992, Nucleotide sequence analysis of the gene encoding the Caulobacter crescentus ... Smit, J., Engelhardt, H., Volker, S., Smith, S.H., and Baumeister, W., 1992, The Slayer of Caulobacter crescentus: Three- ...
The Caulobacter crescentus flagellar gene, fliX, encodes a novel trans-acting factor that couples flagellar assembly to ... The first flagellar assembly checkpoint of Caulobacter crescentus couples assembly of the early class II components of the ...
From left to right: i) The number of proteins in the reference proteome of Caulobacter crescentus, ii) the total number of ... The bar plot shows the coverage for every protein in the reference proteome of Caulobacter crescentus for which there is at ... Caulobacter crescentus is a Gram-negative, oligotrophic bacterium widely distributed in freshwater lakes and streams. ... The plot shows the evolution over years (x-axis) of the fraction of Caulobacter crescentus reference proteome residues (y-axis ...
... and cell division in stalked cells of the α-proteobacterium Caulobacter crescentus. The model is formulated in terms of ... for the spatial asymmetry of Caulobacter reproduction (swarmer cells as well as stalked cells), the correlation of cell growth ... nonlinear ordinary differential equations for the major cell cycle regulatory proteins in Caulobacter: CtrA, GcrA, DnaA, CcrM, ... Caulobacter crescentus Is the Subject Area "Caulobacter crescentus" applicable to this article? Yes. No. ...
Apparently, C. crescentus is not well adapted for efficient growth on high xylose levels, and responds by an extended lag phase ... C. crescentus was grown on xylose, arabinose and glucose, and maximum specific growth rates determined for the three substrates ... Caulobacter crescentus is a gram-negative bacterium that can utilize xylose as a substrate using the Weimberg pathway, which ... Caulobacter crescentus; Weimberg pathway; xylose; arabinose; physiological characterization; enzyme activity Caulobacter ...
Caulobacter crescentus in Biotechnology. From MicrobeWiki, the student-edited microbiology resource. Revision as of 05:51, 15 ... Caulobacter crescentus is a non-pathogenic, aquatic, Gram-negative bacterium.[9] One of its most distinguishing characteristics ... C. crescentus and Pseudomonas aeruginosa vaccine candidate. The novel protein secretion system of C. crescentus was used to ... C. crescentus and HIV. The display capabilities of C. crescentus can be used to develop antibodies, which can mimic host ...
Nucleotide excision repair - Caulobacter crescentus CB15 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
Caulobacter vibrioides (Caulobacter crescentus). Caulobacter sp. 410. Caulobacter flavus. Caulobacter sp. FWC26. 231. UniRef90_ ... Caulobacter crescentus (strain ATCC 19089 / CB15). 231. UniRef100_B8H627. Cluster: Ribonuclease 3. 2. ... sp,B8H627,RNC_CAUCN Ribonuclease 3 OS=Caulobacter crescentus (strain NA1000 / CB15N) OX=565050 GN=rnc PE=3 SV=1 ... Caulobacter vibrioides › Caulobacter crescentus (strain ATCC 19089 / CB15) ...
... small bacterial cells was developed and used to identify chemoreceptor arrays in cryotomograms of intact Caulobacter crescentus ... Location and Architecture of the Caulobacter Crescentus Chemoreceptor Array Mol Microbiol. 2008 Jul;69(1):30-41. doi: 10.1111/j ... The arrays were always found on the convex side of the cell, further demonstrating that Caulobacter cells maintain dorsal/ ... small bacterial cells was developed and used to identify chemoreceptor arrays in cryotomograms of intact Caulobacter crescentus ...
Caulobacter vibrioides (Caulobacter crescentus). Caulobacter sp. 410. Caulobacter flavus. Caulobacter sp. FWC2. Caulobacter sp ... Caulobacter sp. X. Caulobacter sp. FWC26. Caulobacter segnis (strain ATCC 21756 / DSM 7131 / JCM 7823 / NBRC 15250 / LMG 17158 ... Caulobacter crescentus (strain ATCC 19089 / CB15). 358. UniRef100_B8GZ33. Cluster: Modification methylase CcrMI. 2. ... sp,B8GZ33,MTC1_CAUCN Modification methylase CcrMI OS=Caulobacter crescentus (strain NA1000 / CB15N) OX=565050 GN=ccrMIM PE=3 SV ...
The Genetic Basis of Laboratory Adaptation in Caulobacter crescentus Melissa E. Marks, Cyd Marie Castro-Rojas, Clotilde Teiling ... The Genetic Basis of Laboratory Adaptation in Caulobacter crescentus Melissa E. Marks, Cyd Marie Castro-Rojas, Clotilde Teiling ... The Genetic Basis of Laboratory Adaptation in Caulobacter crescentus Melissa E. Marks, Cyd Marie Castro-Rojas, Clotilde Teiling ... The Genetic Basis of Laboratory Adaptation in Caulobacter crescentus Message Subject (Your Name) has forwarded a page to you ...
... of the essential transcriptional regulator CtrA is necessary to drive cell cycle progression in Caulobacter crescentus. At the ... Mutations that alter RcdA surface residues decouple protein localization and CtrA proteolysis in Caulobacter crescentus J Mol ... of the essential transcriptional regulator CtrA is necessary to drive cell cycle progression in Caulobacter crescentus. At the ... We assayed the ability of each RcdA variant to support CtrA proteolysis and polar protein localization in Caulobacter. Deletion ...
Expression of degradative genes of Pseudomonas putida in Caulobacter crescentus.. D K Chatterjee, P Chatterjee ... Expression of degradative genes of Pseudomonas putida in Caulobacter crescentus. Message Subject (Your Name) has forwarded a ... The recombinant plasmid RP4-TOL was transferred into Caulobacter crescentus at a high frequency, and the plasmid was maintained ... C. crescentus cells which contained RP4-TOL grew on all the aromatic compounds that the plasmid normally allowed Pseudomonas ...
... Author(s). Chen, Yiyin Erin ... In this work, I use the model organism Caulobacter crescentus to investigate how intracellular asymmetry within the mother cell ... Caulobacter is an alpha-proteobacterium that always divides asymmetrically to generate two daughter cells that are ... The genetic separability of the spatial and temporal controls of replication in Caulobacter suggests that DnaA comprises an ...
... from the cytoplasm to the cell wall to regulate key developmental shape changes in the α-proteobacterium Caulobacter crescentus ... Caulobacter crescentus Is the Subject Area "Caulobacter crescentus" applicable to this article? Yes. No. ...
An image of slice 210 from the reconstructed volume of a tomographic data set from Caulobacter crescentus. This image has been ... An image of slice 210 from the reconstructed volume of a tomographic data set from Caulobacter crescentus. This image has been ... Caulobacter cultures (strain 3724) were cryofixed using a cocktail of 2% Osmium tetroxide, 0.5% uranyl acetate in anhydrous ... Lucy Shapiro, Harley McAdams (2012) CIL:40007, Caulobacter crescentus CB15. CIL. Dataset. https://doi.org/doi:10.7295/ ...
What is Caulobacter crescentus? Meaning of Caulobacter crescentus medical term. What does Caulobacter crescentus mean? ... Looking for online definition of Caulobacter crescentus in the Medical Dictionary? Caulobacter crescentus explanation free. ... Caulobacter crescentus. Also found in: Encyclopedia, Wikipedia. Caulobacter crescentus. (kawl-ŏ-băk′tĕr krĕ-sĕn′tŭs) A single- ... Caulobacter crescentus , definition of Caulobacter crescentus by Medical dictionary https://medical-dictionary. ...
... from Caulobacter crescentus CB15 at 2.83 A resolution (PSI Community Target, Shapiro) ... Caulobacter crescentus (strain ATCC 19089 / CB15). N/A. Find proteins for Q9A712 (Caulobacter crescentus (strain ATCC 19089 / ... Crystal structure of a putative outer membrane chaperone (OmpH-like) (CC_1914) from Caulobacter crescentus CB15 at 2.83 A ... Crystal structure of a putative outer membrane chaperone (OmpH-like) (CC_1914) from Caulobacter crescentus CB15 at 2.83 A ...
Physiochemical properties of Caulobacter crescentus holdfast: a localized bacterial adhesive. J Phys Chem B 117:10492-10503. ... In Caulobacter crescentus, c-di-GMP regulates the synthesis of the polar holdfast adhesin during the cell cycle, yet the ... We use Caulobacter crescentus as a model to study the regulatory mechanisms of the motile-to-sessile transition of bacteria. ... The HfaB and HfaD adhesion proteins of Caulobacter crescentus are localized in the stalk. Mol Microbiol 49:1671-1683. doi: ...
iv) In their interspecies killing assays with Caulobacter crescentus Cdz producers, the authors have used two closely related α ... A C. crescentus bacteriocin gene cluster is induced in stationary phase. We hypothesized that, if they exist, Caulobacter genes ... Unlike in Caulobacter crescentus, most of these putative contact-dependent systems were predicted to encode a single Cdz-like ... 2010) A modular BAM complex in the outer membrane of the alpha-proteobacterium caulobacter crescentus PLoS One 5:e8619. ...
The Caulobacter crescentus GTPase CgtA C is required for progression through the cell cycle and for maintaining 50S ribosomal ... Citation: Datta, Kaustuv; Skidmore, Jennifer M . ; Pu, Kun; Maddock, Janine R . (2004). "The Caulobacter crescentus GTPase CgtA ... we generated mutations in the Caulobacter crescentus obg gene ( cgtA C ) which, in Ras-like proteins, would result in either ... In C. crescentus , a P168V mutant is not activating in vivo , although in vitro , the P168V protein showed a modest reduction ...
Dynamics and control of biofilms of the oligotrophic bacterium Caulobacter crescentus.. Title. Dynamics and control of biofilms ... Caulobacter crescentus is an oligotrophic alpha-proteobacterium with a complex cell cycle involving sessile-stalked and ... Bacterial Adhesion, Biofilms, Caulobacter crescentus, Culture Media, Fimbriae, Bacterial, Flagella, Fresh Water, Gene ... The involvement of pili in mushroom architecture is a novel function for type IV pili in C. crescentus. These unique biofilm ...
We describe the identification of 27 novel Caulobacter crescentus sRNAs by analysis of RNA expression levels assayed using a ... tiled Caulobacter microarray and a protocol optimized for detection of sRNAs. The principal analysis method involved ... Small non-coding RNAs in Caulobacter crescentus.. @article{Landt2008SmallNR, title={Small non-coding RNAs in Caulobacter ... Iron Deficiency Generates Oxidative Stress and Activation of the SOS Response in Caulobacter crescentus. *Laura Leaden, Larissa ...
Purchase Recombinant Caulobacter crescentus Probable flagellum biosynthesis repressor protein flbT(flbT). It is produced in ... Recombinant Caulobacter crescentus Probable flagellum biosynthesis repressor protein flbT(flbT). Recombinant Caulobacter ... Recombinant Caulobacter crescentus Probable flagellum biosynthesis repressor protein flbT(flbT),Baculovirus. Code. CSB- ... Recombinant Caulobacter crescentus Probable flagellum biosynthesis repressor protein flbT(flbT),E.coli. ...
Caulobacter crescentus is a model organism for the study of asymmetric division and cell type differentiation, as its cell ... Although many genes, proteins, and other molecules involved in the asymmetric division exhibited by C. Crescentus have been ... crescentus. These models provide useful tools for the characterization and analysis of other complex biological networks. ...
  • In the laboratory, researchers distinguish between C. crescentus strain CB15 (the strain originally isolated from a freshwater lake) and NA1000 (the primary experimental strain). (wikipedia.org)
  • The Caulobacter CB15 genome has 4,016,942 base pairs in a single circular chromosome encoding 3,767 genes. (wikipedia.org)
  • In 2010, the Caulobacter NA1000 strain was sequenced and all differences with the CB15 "wild type" strain were identified. (wikipedia.org)
  • The genetic basis of phenotypic differences in growth rate, mucoidy, adhesion, sedimentation, phage susceptibility, and stationary-phase survival between C. crescentus strain CB15 and its derivative NA1000 is determined by coding, regulatory, and insertion/deletion polymorphisms at five chromosomal loci. (asm.org)
  • In an effort to comprehensively characterize their divergence, we identified the genetic basis of all known phenotypic differences between two laboratory strains (NA1000 and CB15) of C. crescentus . (asm.org)
  • CIL:40007, Caulobacter crescentus CB15. (cellimagelibrary.org)
  • Lucy Shapiro, Harley McAdams (2012) CIL:40007, Caulobacter crescentus CB15. (cellimagelibrary.org)
  • Copper-Zinc superoxide dismutase from Caulobacter crescentus CB15. (thefreedictionary.com)
  • In contrast to biofilms of the well-studied Pseudomonas aeruginosa, Escherichia coli, and Vibrio cholerae, C. crescentus CB15 cells form biphasic biofilms, consisting predominantly of a cell monolayer biofilm and a biofilm containing densely packed, mushroom-shaped structures. (stanford.edu)
  • Due to this capacity to be physically synchronized, strain NA1000 has become the predominant experimental Caulobacter strain throughout the world. (wikipedia.org)
  • Comparing the transcriptional profiles of a Caulobacter crescentus CB15N (NA1000) wild-type to a SpoT null in carbon starvation. (omicsdi.org)
  • The S-layer was extracted from the cell surface of C. crescentus NA1000 by treating cells with a pH 2 solution or a solution containing 10 mM ethylene glycol-bis(ß-aminoethylether)- N,N,N',N'-tetraacetic acid (EGTA). (ubc.ca)
  • Two mutants of C. crescentus NA1000, JS1001 and JS1002, selected for the ability to grow in the absence of calcium had the additional phenotype of being unable to attach the S-layer to the cell surface although they produced a wild-type RsaA. (ubc.ca)
  • Caulobacter crescentus NA1000 possess two HME-RND proteins, and the aim of this work was to determine their involvement in the response to cadmium, zinc, cobalt and nickel, and to analyze the phylogenetic distribution and characteristic signatures of orthologs of these two proteins. (beds.ac.uk)
  • Using a digital tracking microscope that provides both cell position and orientation, we have correlated the detailed motion of the cell body of a fast-swimming bacterium, Caulobacter crescentus , with its swimming motility. (pnas.org)
  • We have used this technique to study the motility of the uniflagellated bacterium Caulobacter crescentus and have found that each cell displays two distinct modes of motility, depending on the sense of rotation of the flagellar motor. (pnas.org)
  • The dimorphic bacterium Caulobacter crescentus has evolved marked phenotypic changes during its 50-year history of culture in the laboratory environment, providing an excellent system for the study of natural selection and phenotypic microevolution in prokaryotes. (asm.org)
  • Likewise, the aquatic bacterium Caulobacter crescentus has evolved marked phenotypic changes during the 50 years it has been cultured in the laboratory environment. (asm.org)
  • The researchers started the project by trying to understand why the bacterium Caulobacter crescentus produces two distinct types of cell - one with a flagellum (appendage) and the other without. (thefreedictionary.com)
  • Dynamics and control of biofilms of the oligotrophic bacterium Caulobacter crescentus. (stanford.edu)
  • However, even that does not compare to what the bacterium Caulobacter crescentus can do. (listverse.com)
  • A protein critical for cell constriction in the Gram-negative bacterium Caulobacter crescentus localizes at the division site through its peptidoglycan-binding LysM domains. (helmholtz-hzi.de)
  • In order to gain insight about the material properties of bacterial adhesins, we study the morphogenesis of the adhesive holdfast of the Gram negative bacterium Caulobacter crescentus . (biomedcentral.com)
  • Microarray analysis was used to examine gene expression in the freshwater oligotrophic bacterium Caulobacter crescentus during growth on three standard laboratory media, including peptone-yeast extract medium (PYE) and minimal salts medium with glucose or xylose as the carbon source. (scu.edu)
  • CAULOBACTERThe common waterborne bacterium Caulobacter crescentus reproduces asymmetrically. (amazonaws.com)
  • In the article, "Obstruction of Pilus Retraction Stimulates Bacterial Surface Sensing," a group of interdisciplinary researchers show that in the case of the bacterium Caulobacter crescentus , a model organism that splits its life between swimming and attaching to surfaces, physically blocking the retraction of its pili triggers the attachment to surfaces. (eurekalert.org)
  • Scientists looked at the bacterium Caulobacter crescentus . (hubpages.com)
  • In the asymmetrically dividing bacterium Caulobacter crescentus , cell polarity stems from the cell cycle-regulated localization and turnover of signaling protein complexes in these hubs, and yet the mechanisms that establish the identity of the two cell poles have not been established. (asm.org)
  • The asymmetrically dividing bacterium Caulobacter crescentus uses one such microdomain to link cell cycle progression to morphogenesis, but the mechanism for the generation of this microdomain has remained unclear. (asm.org)
  • The asymmetrically dividing bacterium Caulobacter crescentus is a model system for single-cell polarity, as every cell division produces two daughter cells that differ in their morphology, replication competency, and size ( 1 ). (asm.org)
  • Forward genetics and super-resolution microscopy identifies ZitP as a conserved multifunctional regulator that accumulates at both cell extremities in distinct macromolecular structures to perform different functions in the asymmetric model bacterium Caulobacter crescentus . (elifesciences.org)
  • Triplicate cultures of: 1) C. crescentus strain CB15N , and 2) CB15N ∆SpoT, cultured in M2-glucose media, then starved for glucose for 5 minutes. (omicsdi.org)
  • C. crescentus is, to our knowledge, the first free-living α-class proteobacterium to be sequenced and will serve as a foundation for exploring the biology of this group of bacteria, which includes the obligate endosymbiont and human pathogen Rickettsia prowazekii , the plant pathogen Agrobacterium tumefaciens , and the bovine and human pathogen Brucella abortus . (pnas.org)
  • The genetic separability of the spatial and temporal controls of replication in Caulobacter suggests that DnaA comprises an ancient and phylogenetically widespread control module for replication in almost all bacteria while CtrA developed later in c-proteobacteria and was recruited to enforce replicative asymmetry in daughter cells. (mit.edu)
  • Partially purified bacterial extracts were prepared from segregated stalked and swarmer bacteria of Caulobacter crescentus and assayed for ATP-dependent deoxyribonuclease activity. (microbiologyresearch.org)
  • This activity was found to be very low in the swarmer bacteria compared with a pure population of stalked cells or an asynchronous population of C. crescentus. (microbiologyresearch.org)
  • [2] Since C. crescentus move in places where there is not much to eat, it is believed that these bacteria also use their superglue to pick up nutrients. (listverse.com)
  • We report the response of C. crescentus to carbon starvation, a common form of nutritional stress encountered by free-living bacteria, that induces stasis. (omicsdi.org)
  • Instead of dividing two form two identical daughter cells as other bacteria do (a process termed binary division), Caulobacter crescentus undergoes what is termed symmetric division. (encyclopedia.com)
  • Caulobacter crescentus can be grown in the laboratory so that all the bacteria in the population undergoes division at the same time. (encyclopedia.com)
  • Like many bacteria, Caulobacter responds to phosphate limitation through a conserved two-component signaling pathway called PhoR-PhoB, but the direct regulon of PhoB in this organism is unknown. (datamed.org)
  • Drawing from his engineering background, Princeton University researcher Alexandre Persat had a notion as to why the bacteria Caulobacter crescentus are curved - a hunch that now could lead to a new way of studying the evolution of bacteria, according to research published in the journal Nature Communications . (innovations-report.com)
  • Princeton University researchers found that the banana-like curve of the bacteria Caulobacter crescentus provides stability and helps them flourish as a group in the moving water they experience in nature. (innovations-report.com)
  • While most bacteria grow and divide as two identical "daughter" cells, C. crescentus divides asymmetrically. (innovations-report.com)
  • Moreover, the restriction enzyme digestion maps of RP4-TOL from both C. crescentus and P. putida were the same, indicating that the expression of the TOL genes occurred without any apparent alteration of the gene structure. (asm.org)
  • To test this hypothesis, we generated mutations in the Caulobacter crescentus obg gene ( cgtA C ) which, in Ras-like proteins, would result in either activating or dominant negative phenotypes. (umich.edu)
  • Polarity of gene transfer in Caulobacter. (microbiologyresearch.org)
  • Here, we examine if selection acts on G+C contents in Caulobacter crescentus and Pseudomonas aeruginosa , which both have very G+C-rich genomes, by testing whether the expression of gene variants that differ only in their base compositions at synonymous sites affects cellular growth rates. (g3journal.org)
  • In C. crescentus , expression of the more A+T-rich gene variants decelerated growth, indicating that selection on genic base composition is, in part, responsible for the high G+C content of this genome. (g3journal.org)
  • Thus, during C. crescentus flagellar biogenesis two different regulatory checkpoints link structural assembly to flagellar gene expression. (beds.ac.uk)
  • However, our studies show that it likely does not play such a role in Caulobacter as depleting PhoU has no significant effect on PhoB-dependent gene expression. (datamed.org)
  • The goal of our project is to clone the gene, mreB, from ''Caulobacter crescentus'' (stalked shaped cell) into ''Escherchia coli'' (rod shaped cell) to see if mreB will affect ''E. coli's'' cell shape in some way. (openwetware.org)
  • This report suggests that another outer membrane component, termed the S-layer-associated oligosaccharide (SAO), is the molecule responsible for the attachment of RsaA, the S-layer protein of Caulobacter crescentus , to the cell surface. (springer.com)
  • From left to right: i) The number of proteins in the reference proteome of Caulobacter crescentus , ii) the total number of models, iii) the number of unique protein sequences for which at least one model is available and iv) a coverage bar plot is shown. (expasy.org)
  • The bar plot shows the coverage for every protein in the reference proteome of Caulobacter crescentus for which there is at least one model. (expasy.org)
  • [1] The S-layer is composed a single protein, RsaA, and is among the most abundant proteins in C. crescentus , accounting for 31% of total cell protein, and up to 51% when multiple copies of rsaA are present. (kenyon.edu)
  • Due to the non-pathogenic nature of C. crescentus [3] and its high expression of RsaA protein, it has been used to express and assemble foreign proteins at high concentrations. (kenyon.edu)
  • The S-layer protein of C. crescentus has not yet been visualized by X-Ray crystallography . (kenyon.edu)
  • We assayed the ability of each RcdA variant to support CtrA proteolysis and polar protein localization in Caulobacter. (nih.gov)
  • Here, we identify an atypical two-protein bacteriocin in the α-proteobacterium Caulobacter crescentus that is retained on the surface of producer cells where it mediates cell contact-dependent killing. (elifesciences.org)
  • These aggregates can drive contact-dependent killing of other organisms, or Caulobacter cells not producing the CdzI immunity protein. (elifesciences.org)
  • In C. crescentus , a P168V mutant is not activating in vivo , although in vitro , the P168V protein showed a modest reduction in the affinity for GDP. (umich.edu)
  • Caulobacter crescentus nucleoid: analysis of sedimentation behavior and protein composition during the cell cycle. (microbiologyresearch.org)
  • The method by which the protein subunits composing the S-layer of C. crescentus, RsaA, interact to form the array and attach to the cell was examined in this thesis. (ubc.ca)
  • We used high-throughput PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in live Caulobacter crescentus. (umn.edu)
  • Here, using genome-wide methods and microscopy of single cells, we show that Caulobacter SMC is recruited to the centromeric parS site and that SMC-mediated arm alignment depends on the chromosome-partitioning protein ParB. (semanticscholar.org)
  • Three-dimensional superresolution colocalization of intracellular protein superstructures and the cell surface in live Caulobacter crescentus. (mendeley.com)
  • Phylogenetic analysis of the C. crescentus HME-RND proteins showed that CzrA-like proteins, in contrast to those similar to NczA, are almost exclusively found in the Alphaproteobacteria group, and the characteristic protein signatures of each group were highlighted. (beds.ac.uk)
  • By comparison to self-assembling protein networks and polar cell growth mechanisms in other bacterial species, we suggest that the cooligomeric PopZ-SpmX protein complex in Caulobacter illustrates a paradigm for coupling cell cycle progression to the controlled geometry of cell pole establishment. (asm.org)
  • Further, we find that overexpression of the bridge protein SpmX in Caulobacter disrupts this ordered assembly, generating ectopic cell poles containing both PopZ and DivJ. (asm.org)
  • In this paper, we investigate the mechanisms governing the localization of PopZ, a chromosome-anchoring protein whose unipolar to bipolar localization pattern is critical for cell cycle progression in Caulobacter crescentus . (rupress.org)
  • Genome analysis revealed that the C. crescentus genome encodes a significantly higher number of these signaling proteins (105) than any bacterial genome sequenced thus far. (pnas.org)
  • The basic paradigm of cell cycle control used by eukaryotic cells, temporally controlled transcription, phosphorylation of regulatory factors, and targeted proteolysis, has been conserved in C. crescentus , although the proteins involved in these processes are different. (pnas.org)
  • Recent observations of regulatory proteins dynamically localized to defined cellular addresses at specific times in the C. crescentus cell cycle suggest that the three-dimensional organization of this cell adds yet another layer of control ( 5 ). (pnas.org)
  • Although many genes, proteins, and other molecules involved in the asymmetric division exhibited by C. Crescentus have been discovered and characterized during decades, it remains as a challenging task to understand how cell properties arise from the high number of interactions between these molecular components. (ginsim.org)
  • The relative copy numbers of these proteins are essential for complex formation, as overexpression of SpmX in Caulobacter reorganizes the polarity of the cell, generating ectopic cell poles containing PopZ and DivJ. (asm.org)
  • Prior to cytokinesis in Caulobacter , distinct sets of signaling proteins localize to opposite cell poles where they dictate the cell fate of the nascent daughter cells. (asm.org)
  • This study evidences multiple genetic mechanisms of bacterial evolution as driven by selection for growth and survival in a new selective environment and identifies a common polymorphic locus, zwf , between lab-adapted C. crescentus and clinical isolates of Pseudomonas aeruginosa that have adapted to a human host during chronic infection. (asm.org)
  • Expression of degradative genes of Pseudomonas putida in Caulobacter crescentus. (asm.org)
  • C. crescentus cells which contained RP4-TOL grew on all the aromatic compounds that the plasmid normally allowed Pseudomonas putida to grow on. (asm.org)
  • can be transferred, maintained, and expressed efficiently in C. crescentus and that the mechanism of transcriptional activation of TOL genes observed in C. crescentus is similar to that of Pseudomonas spp. (asm.org)
  • Transfer and expression of Pseudomonas plasmid RP1 in Caulobacter. (microbiologyresearch.org)
  • Asymmetrical distribution of c-di-GMP in the progeny correlated with the time of cell division and polarization for Caulobacter crescentus and Pseudomonas aeruginosa . (sciencemag.org)
  • Caulobacter crescentus is a Gram-negative, oligotrophic bacterium widely distributed in fresh water lakes and streams. (wikipedia.org)
  • Periodic activation and deactivation of the essential transcriptional regulator CtrA is necessary to drive cell cycle progression in Caulobacter crescentus. (nih.gov)
  • Transcriptional profiling of Caulobacter crescentus during growth on complex and minimal media. (helmholtz-hzi.de)
  • Transcriptional Profiling of Caulobacter crescentus during Growth on C" by Craig Stephens, Alison K. Hottes et al. (scu.edu)
  • We report a study by fluorescence imaging and atomic force microscopy on the growth in size and thickness of the holdfast of synchronized Caulobacter crescentus cells as they attach to a glass surface. (biomedcentral.com)
  • For live-cell imaging of c-di-GMP signaling events in single bacterial cells, we expressed the YcgR-based c-di-GMP sensor in Caulobacter crescentus and measured FRET efficiencies by ratiometric dual-emission microscopy ( Fig. 1, A and B ). Caulobacter has asymmetric cell division, resulting in two morphologically distinct progeny: a surface-attached replication-competent stalk cell, and a motile swarmer cell with a polar flagellum. (sciencemag.org)
  • The complete genome sequence of Caulobacter crescentus was determined to be 4,016,942 base pairs in a single circular chromosome encoding 3,767 genes. (pnas.org)
  • Caulobacter does all that with less than 4,000 genes, allowing full genome-wide studies of a single differentiating cell. (pnas.org)
  • Circular representation of the C. crescentus genome. (pnas.org)
  • The genome of C. crescentus was first sequenced in 2001. (expasy.org)
  • The completion of the entire genome sequences of Chlorobium tepidum and Caulobacter crescentus mark a major milestone in TIGR's history. (thefreedictionary.com)
  • Reciprocal transfers from C. crescentus donor to P. putida or Escherichia coli recipients were less efficient and occurred at frequencies of approximately 10(-3). (asm.org)
  • Cloning vectors for studies of Caulobacter crescentus genes should be transferable between Escherichia coli and C. crescentus since a transformation system has not been developed for C. crescentus. (elsevier.com)
  • The first flagellar assembly checkpoint of Caulobacter crescentus couples assembly of the early class II components of the basal body complex to the expression of class III and IV genes, which encode extracytoplasmic structures of the flagellum. (ingentaconnect.com)
  • These data indicate that R300B does not contain sequences which would provide promoter function in C. crescentus in the orientation opposite to that of the sul operon and that any genes cloned in this orientation would require native promoters for expression. (elsevier.com)
  • Specific clones from these libraries containing genes of interest were identified by complementation of the appropriate C. crescentus mutants. (elsevier.com)
  • Glucose availability induced expression of genes encoding enzymes of the Entner-Doudoroff pathway, which was demonstrated here through mutational analysis to be essential in C. crescentus for growth on glucose. (scu.edu)
  • The temporal and spatial expression of late flagellar genes in Caulobacter crescentus is activated by the transcription factor FlbD and its partner trans-acting factor FliX. (beds.ac.uk)
  • Over fifty genes are required for flagellar biogenesis in C. crescentus , and their temporal and spatial expression is regulated by both cell cycle events and the progression of flagellum assembly. (beds.ac.uk)
  • Caulobacter strains bearing mutations in fliX are non-motile and do not transcribe class III and IV flagellar genes. (beds.ac.uk)
  • The PhoB regulon is comprised primarily of genes known or predicted to help Caulobacter scavenge for and import inorganic phosphate, including 15 different membrane transporters. (datamed.org)
  • For instance, he said, 80 percent of the genes in C. crescentus are seemingly disposable - but they might not be in nature. (innovations-report.com)
  • Caulobacter is an important model organism for studying the regulation of the cell cycle, asymmetric cell division, and cellular differentiation. (wikipedia.org)
  • C. crescentus is a simple and highly manipulable single-celled model system to study cellular differentiation, asymmetric division, and their coordination with cell cycle progression ( 1 , 2 ). (pnas.org)
  • Caulobacter crescentus is a model organism for the study of asymmetric division and cell type differentiation, as its cell division cycle generates a pair of daughter cells that differ from one another in their morphology and behavior. (ginsim.org)
  • Modeling Asymmetric Cell Division in Caulobacter crescentus Using a Boolean Logic Approach. (ginsim.org)
  • The Caulobacter cell cycle regulatory system controls many modular subsystems that organize the progression of cell growth and reproduction. (wikipedia.org)
  • Mutations in highly conserved regions of FliX could severely affect the recognition between FliX and FlbD and hence interrupt the normal progression of flagellar synthesis and other developmental events in Caulobacter . (beds.ac.uk)
  • C. crescentus is an interesting organism to study because it inhabits nutrient-poor aquatic environments. (wikipedia.org)
  • In this work, I use the model organism Caulobacter crescentus to investigate how intracellular asymmetry within the mother cell is translated into the formation of two developmentally distinct daughter cells. (mit.edu)
  • Almqvist H, Jonsdottir Glaser S, Tufvegren C, Wasserstrom L, Lidén G. Characterization of the Weimberg Pathway in Caulobacter crescentus . (mdpi.com)
  • We describe the identification of 27 novel Caulobacter crescentus sRNAs by analysis of RNA expression levels assayed using a tiled Caulobacter microarray and a protocol optimized for detection of sRNAs. (semanticscholar.org)
  • It was originally identified along with 26 other non-coding RNAs using a tiled Caulobacter microarray protocol specifically aimed at detecting small RNAs. (wikipedia.org)
  • Caulobacter daughter cells have two very different forms. (wikipedia.org)
  • Detailed study of the molecular development of these cells as they progress through the cell cycle has enabled researchers to understand Caulobacter cell cycle regulation in great detail. (wikipedia.org)
  • A new method for recording both fluorescence and cryo-EM images of small bacterial cells was developed and used to identify chemoreceptor arrays in cryotomograms of intact Caulobacter crescentus cells. (nih.gov)
  • The arrays were always found on the convex side of the cell, further demonstrating that Caulobacter cells maintain dorsal/ventral as well as anterior/posterior asymmetry. (nih.gov)
  • A) Caulobacter cells divide asymmetrically to yield a swarmer and a stalked cell, which are mixed in culture. (asm.org)
  • The amounts of mRNA from induced cells of C. crescentus(RP4-TOL) and P. putida(RP4-TOL) were also similar. (asm.org)
  • Caulobacter is an alpha-proteobacterium that always divides asymmetrically to generate two daughter cells that are morphologically distinct and have different replicative capacities. (mit.edu)
  • Caulobacter crescentus is an oligotrophic alpha-proteobacterium with a complex cell cycle involving sessile-stalked and piliated, flagellated swarmer cells. (stanford.edu)
  • These unique biofilm features demonstrate a spatial diversification of the C. crescentus population into a sessile, "stem cell"-like subpopulation (monolayer biofilm), which generates progeny cells capable of exploring the aqueous, oligotrophic environment by swimming motility and a subpopulation accumulating in large mushroom structures. (stanford.edu)
  • Envelope- associated nucleoid from Caulobactercrescentus stalked and swarmer cells. (microbiologyresearch.org)
  • C. vibrioides) swarmer and stalked cells starved for carbon GSE25998: Expression data from WT, DSigT and DSigU Caulobacter crescentus (syn. (omicsdi.org)
  • GdhZ co-localizes with FtsZ in late predivisional cells and GdhZ abundance varies during the cell cycle being specifically degraded during DNA replication the period during which the Z-ring is assembled at the division site in C. crescentus. (unamur.be)
  • Caulobacter crescentus undergoes a series of programmed differentiation events within each cell cycle and generates two dissimilar progeny cells, a motile swarmer cell possessing a single polar flagellum and a sessile stalked cell. (beds.ac.uk)
  • The taxon is more properly known as Caulobacter vibrioides (Henrici and Johnson 1935). (wikipedia.org)
  • C. crescentus is synonymous with Caulobacter vibrioides. (wikipedia.org)
  • C. crescentus invariably differentiates and divides asymmetrically at each cell cycle. (pnas.org)
  • The illustration shows how C. crescentus divides asymmetrically into a "stalked" mother cell that anchors to a bacterium's home surface, and an upper unattached portion that forms a new, juvenile cell known as a "swarmer. (innovations-report.com)
  • The involvement of pili in mushroom architecture is a novel function for type IV pili in C. crescentus. (stanford.edu)
  • Then, one end of C. crescentus attaches to the surface in question, after which the bacterium is anchored to it through thin structures called pili. (listverse.com)
  • During their study, the scientists found a new technique to observe and film Caulobacter crescentus pili undergoing dynamic cycles of extension and retraction. (eurekalert.org)
  • Then, a new route for glycolate production was established in E . coli by introducing NAD + -dependent xylose dehydrogenase ( xdh ) and xylonolactonase ( xylC ) from Caulobacter crescentus . (springer.com)
  • The link between the City University of New York and Caulobacter crescentus dates back far before this study, however, as Brooklyn College alumnus Lucy Shapiro pioneered the study of Caulobacter crescentus as a model system. (eurekalert.org)
  • Isolation of mutants of Caulobacter crescentus deficient in ATP-dependent deoxyribonuclease activity. (microbiologyresearch.org)
  • All mutants were successfully expressed in both wild-type and ΔfliX Caulobacter strains. (beds.ac.uk)
  • The recombinant plasmid RP4-TOL was transferred into Caulobacter crescentus at a high frequency, and the plasmid was maintained for at least 50 generations. (asm.org)
  • Analysis of the dynamics of TrfA- complex formation during the Caulobacter cell cycle revealed that TrfA binds primarily during the G1 phase, however plasmid DNA synthesis occurs during the S and G2 phases of the cell cycle. (unibas.ch)
  • DipM links peptidoglycan remodelling to outer membrane organization in Caulobacter. (helmholtz-hzi.de)
  • During initial cancer vaccine studies, unexpected results in the control group showed that wild type C. crescentus displayed anti-tumor properties in three different strains of mice, each challenged with a different transplantable tumor model. (kenyon.edu)
  • Summary: When Caulobacter crescentus enters S-phase the replication initiation inhibitor CtrA dynamically positions to the old cell pole to be degraded by the polar ClpXP protease. (elsevier.com)
  • Together these data indicate that PopA adopted a novel role as topology specificity factor to help recruit components of the CtrA degradation pathway to the protease specific old cell pole of C. crescentus. (elsevier.com)
  • Selection for increased genic G+C-contents in C. crescentus acts independently of the species-specific codon usage pattern and represents an additional selective force operating in bacterial genomes. (g3journal.org)
  • These projects would not be restricted to bacterial pathogens but would also encompass non-pathogenic bacterial species such as Caulobacter crescentus. (ncl.ac.uk)
  • In Caulobacter crescentus , c-di-GMP regulates the synthesis of the polar holdfast adhesin during the cell cycle, yet the molecular and cellular details of this control are currently unknown. (asm.org)
  • Here we describe a novel c-di-GMP effector, HfsK, that contributes to the cohesive properties and stability of the holdfast adhesin in C. crescentus . (asm.org)
  • The Caulobacter stalked cell stage provides a fitness advantage by anchoring the cell to surfaces to form biofilms and or to exploit nutrient sources. (wikipedia.org)
  • Chromosome replication in Caulobacter crescentus growing in a nutrient broth. (microbiologyresearch.org)
  • Altogether this work illustrates how C. crescentus can adjust cell cycle parameters according to nutrient availability fluctuations. (unamur.be)
  • Yet, Caulobacter has the swarmer cell stage that results in slower population growth. (wikipedia.org)
  • C. crescentus was grown on xylose, arabinose and glucose, and maximum specific growth rates determined for the three substrates were 0.11 h −1 , 0.05 h −1 , and 0.15 h −1 respectively. (mdpi.com)
  • Autolysis of Caulobacter crescentus grown in the presence of glycine. (helmholtz-hzi.de)
  • This study is measuring the steady-state levels of mRNA in wild-type Caulobacter crescentus grown in M2 defined medium containing either ammonium or nitrate as the sole nitrogen source. (omicsdi.org)
  • Four independent cultures of Caulobacter crecentus were grown in each of two medium conditions: M2(nitrate)glucose and M2(ammonium)glucose. (omicsdi.org)
  • Interestingly, deletion of gdhZ homolog in the facultative intracellular pathogen Brucella abortus (gdhZba) leads to cell division defects, similarly to Caulobacter ∆gdhZ. (unamur.be)
  • To quantitatively explore the correlation between cell kinematics and motility, we use a 3D tracking microscope-a digital implementation of the instrument first developed by Berg ( 11 )-to follow individuals of Caulobacter crescentus for extended periods of time. (pnas.org)
  • That means that their curvature could be an adaptation that allows C. crescentus to better develop in the water currents the organisms experience in nature. (innovations-report.com)
  • Caulobacter crescentus is a gram-negative bacterium that can utilize xylose as a substrate using the Weimberg pathway, which converts xylose to α -ketoglutarate in five steps without carbon loss. (mdpi.com)
  • Small non-coding RNAs in Caulobacter crescentus. (semanticscholar.org)
  • CrfA RNA (Caulobacter response to famine RNA) is a family of non-coding RNAs found in Caulobacter crescentus. (wikipedia.org)
  • [3] In the Lewis lung carcinoma model, 60% of the mice pre-immunized with live C. crescentus were still alive by day 90, whereas all mice pre-immunized with heat killed C. crescentus or the saline control died by 8 weeks post tumor challenge. (kenyon.edu)
  • [3] In the murine L1210 leukemia model, the entire control group only survived until the fourth week, whereas approximately 80% of mice pre-immunized with live C. crescentus survived, and 100% of the heat-killed immunized mice survived the entire 3-month study period. (kenyon.edu)
  • flgH), a model for biofilm formation in C. crescentus is proposed. (stanford.edu)
  • Glucose, the only carbon source in the minimal media M2G was removed from C. crescentus exponential phase cultures, and total RNA was extracted after 30 and 60 minutes of incubation. (omicsdi.org)
  • CrfA was found to be upregulated 10-fold in C. crescentus when glucose was deprived in a minimal medium. (wikipedia.org)