A species of gram-negative, aerobic bacteria that consist of slender vibroid cells.
A genus of gram-negative, aerobic, rod- or vibroid-shaped or fusiform bacteria that commonly produce a stalk. They are found in fresh water and soil and divide by binary transverse fission.
A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
Proteins found in any species of bacterium.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
A protein with a molecular weight of 40,000 isolated from bacterial flagella. At appropriate pH and salt concentration, three flagellin monomers can spontaneously reaggregate to form structures which appear identical to intact flagella.
The functional hereditary units of BACTERIA.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
A class in the phylum PROTEOBACTERIA comprised mostly of two major phenotypes: purple non-sulfur bacteria and aerobic bacteriochlorophyll-containing bacteria.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Physiological processes and properties of BACTERIA.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
An enzyme responsible for producing a species-characteristic methylation pattern on adenine residues in a specific short base sequence in the host cell DNA. The enzyme catalyzes the methylation of DNA adenine in the presence of S-adenosyl-L-methionine to form DNA containing 6-methylaminopurine and S-adenosyl-L-homocysteine. EC 2.1.1.72.
A large group of aerobic bacteria which show up as pink (negative) when treated by the gram-staining method. This is because the cell walls of gram-negative bacteria are low in peptidoglycan and thus have low affinity for violet stain and high affinity for the pink dye safranine.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The process by which a DNA molecule is duplicated.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
A DNA-directed RNA polymerase found in BACTERIA. It is a holoenzyme that consists of multiple subunits including sigma factor 54.
Viruses whose hosts are bacterial cells.
An ATP-dependent protease found in prokaryotes, CHLOROPLASTS, and MITOCHONDRIA. It is a soluble multisubunit complex that plays a role in the degradation of many abnormal proteins.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
A potassium salt used to replenish ELECTROLYTES, for restoration of WATER-ELECTROLYTE BALANCE, as well as a urinary and systemic alkalizer, which can be administered orally or by intravenous infusion. Formerly, it was used in DIURETICS and EXPECTORANTS.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Tungsten hydroxide oxide phosphate. A white or slightly yellowish-green, slightly efflorescent crystal or crystalline powder. It is used as a reagent for alkaloids and many other nitrogen bases, for phenols, albumin, peptone, amino acids, uric acid, urea, blood, and carbohydrates. (From Merck Index, 11th ed)
A flavoring agent. It is the intermediate product in the two-step bioconversion of ferulic acid to vanillin. (J Biotechnol 1996;50(2-3):107-13).
A unique DNA sequence of a replicon at which DNA REPLICATION is initiated and proceeds bidirectionally or unidirectionally. It contains the sites where the first separation of the complementary strands occurs, a primer RNA is synthesized, and the switch from primer RNA to DNA synthesis takes place. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
A protein which is a subunit of RNA polymerase. It effects initiation of specific RNA chains from DNA.
D-Galactose:NAD(P)+ 1-oxidoreductases. Catalyzes the oxidation of D-galactose in the presence of NAD+ or NADP+ to D-galactono-gamma-lactone and NADH or NADPH. Includes EC 1.1.1.48 and EC 1.1.1.120.
Enzymes that catalyze the cleavage of a phosphorus-oxygen bond by means other than hydrolysis or oxidation. EC 4.6.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.

Chromosome methylation and measurement of faithful, once and only once per cell cycle chromosome replication in Caulobacter crescentus. (1/444)

Caulobacter crescentus exhibits cell-type-specific control of chromosome replication and DNA methylation. Asymmetric cell division yields a replicating stalked cell and a nonreplicating swarmer cell. The motile swarmer cell must differentiate into a sessile stalked cell in order to replicate and execute asymmetric cell division. This program of cell division implies that chromosome replication initiates in the stalked cell only once per cell cycle. DNA methylation is restricted to the predivisional cell stage, and since DNA synthesis produces an unmethylated nascent strand, late DNA methylation also implies that DNA near the replication origin remains hemimethylated longer than DNA located further away. In this report, both assumptions are tested with an engineered Tn5-based transposon, Tn5Omega-MP. This allows a sensitive Southern blot assay that measures fully methylated, hemimethylated, and unmethylated DNA duplexes. Tn5Omega-MP was placed at 11 sites around the chromosome and it was clearly demonstrated that Tn5Omega-MP DNA near the replication origin remained hemimethylated longer than DNA located further away. One Tn5Omega-MP placed near the replication origin revealed small but detectable amounts of unmethylated duplex DNA in replicating stalked cells. Extra DNA synthesis produces a second unmethylated nascent strand. Therefore, measurement of unmethylated DNA is a critical test of the "once and only once per cell cycle" rule of chromosome replication in C. crescentus. Fewer than 1 in 1,000 stalked cells prematurely initiate a second round of chromosome replication. The implications for very precise negative control of chromosome replication are discussed with respect to the bacterial cell cycle.  (+info)

The CtrA response regulator mediates temporal control of gene expression during the Caulobacter cell cycle. (2/444)

In its role as a global response regulator, CtrA controls the transcription of a diverse group of genes at different times in the Caulobacter crescentus cell cycle. To understand the differential regulation of CtrA-controlled genes, we compared the expression of two of these genes, the fliQ flagellar gene and the ccrM DNA methyltransferase gene. Despite their similar promoter architecture, these genes are transcribed at different times in the cell cycle. PfliQ is activated earlier than PccrM. Phosphorylated CtrA (CtrA approximately P) bound to the CtrA recognition sequence in both promoters but had a 10- to 20-fold greater affinity for PfliQ. This difference in affinity correlates with temporal changes in the cellular levels of CtrA. Disrupting a unique inverted repeat element in PccrM significantly reduced promoter activity but not the timing of transcription initiation, suggesting that the inverted repeat does not play a major role in the temporal control of ccrM expression. Our data indicate that differences in the affinity of CtrA approximately P for PfliQ and PccrM regulate, in part, the temporal expression of these genes. However, the timing of fliQ transcription but not of ccrM transcription was altered in cells expressing a stable CtrA derivative, indicating that changes in CtrA approximately P levels alone cannot govern the cell cycle transcription of these genes. We propose that changes in the cellular concentration of CtrA approximately P and its interaction with accessory proteins influence the temporal expression of fliQ, ccrM, and other key cell cycle genes and ultimately the regulation of the cell cycle.  (+info)

Cell cycle-dependent polar localization of an essential bacterial histidine kinase that controls DNA replication and cell division. (3/444)

The master CtrA response regulator functions in Caulobacter to repress replication initiation in different phases of the cell cycle. Here, we identify an essential histidine kinase, CckA, that is responsible for CtrA activation by phosphorylation. Although CckA is present throughout the cell cycle, it moves to a cell pole in S phase, and upon cell division it disperses. Removal of the membrane-spanning region of CckA results in loss of polar localization and cell death. We propose that polar CckA functions to activate CtrA just after the initiation of DNA replication, thereby preventing premature reinitiations of chromosome replication. Thus, dynamic changes in cellular location of critical signal proteins provide a novel mechanism for the control of the prokaryote cell cycle.  (+info)

Identification and transcriptional control of the genes encoding the Caulobacter crescentus ClpXP protease. (4/444)

The region of the Caulobacter crescentus chromosome harboring the genes for the ClpXP protease was isolated and characterized. Comparison of the deduced amino acid sequences of the C. crescentus ClpP and ClpX proteins with those of their homologues from several gram-positive and gram-negative bacteria revealed stronger conservation for the ATPase regulatory subunit (ClpX) than for the peptidase subunit (ClpP). The C. crescentus clpX gene was shown by complementation analysis to be functional in Escherichia coli. However, clpX from E. coli was not able to substitute for the essential nature of the clpX gene in C. crescentus. The clpP and clpX genes are separated on the C. crescentus chromosome by an open reading frame pointing in the opposite direction from the clp genes, and transcription of clpP and clpX was found to be uncoupled. clpP is transcribed as a monocistronic unit with a promoter (PP1) located immediately upstream of the 5' end of the gene and a terminator structure following its 3' end. PP1 is under heat shock control and is induced upon entry of the cells into the stationary phase. At least three promoters for clpX (PX1, PX2, and PX3) were mapped in the clpP-clpX intergenic region. In contrast to PP1, the clpX promoters were found to be downregulated after heat shock but were also subject to growth phase control. In addition, the clpP and clpX promoters showed different activity patterns during the cell cycle. Together, these results demonstrate that the genes coding for the peptidase and the regulatory subunits of the ClpXP protease are under independent transcriptional control in C. crescentus. Determination of the numbers of ClpP and ClpX molecules per cell suggested that ClpX is the limiting component compared with ClpP.  (+info)

Cell cycle expression and transcriptional regulation of DNA topoisomerase IV genes in caulobacter. (5/444)

DNA replication and differentiation are closely coupled during the Caulobacter crescentus cell cycle. We have previously shown that DNA topoisomerase IV (topo IV), which is encoded by the parE and parC genes, is required for chromosomal partitioning, cell division, and differentiation in this bacterium (D. Ward and A. Newton, Mol. Microbiol. 26:897-910, 1997). We have examined the cell cycle regulation of parE and parC and report here that transcription of these topo IV genes is induced during the swarmer-to-stalked-cell transition when cells prepare for initiation of DNA synthesis. The regulation of parE and parC expression is not strictly coordinated, however. The rate of parE transcription increases ca. 20-fold during the G1-to-S-phase transition and in this respect, its pattern of regulation is similar to those of several other genes required for chromosome duplication. Transcription from the parC promoter, by contrast, is induced only two- to threefold during this cell cycle period. Steady-state ParE levels are also regulated, increasing ca. twofold from low levels in swarmer cells to a maximum immediately prior to cell division, while differences in ParC levels during the cell cycle could not be detected. These results suggest that topo IV activity may be regulated primarily through parE expression. The presumptive promoters of the topo IV genes display striking similarities to, as well as differences from, the consensus promoter recognized by the major Caulobacter sigma factor sigma73. We also present evidence that a conserved 8-mer sequence motif located in the spacers between the -10 and -35 elements of the parE and parC promoters is required for maximum levels of parE transcription, which raises the possibility that it may function as a positive regulatory element. The pattern of parE transcription and the parE and parC promoter architecture suggest that the topo IV genes belong to a specialized subset of cell cycle-regulated genes required for chromosome replication.  (+info)

Feedback control of a master bacterial cell-cycle regulator. (6/444)

The transcriptional regulator CtrA controls several key cell-cycle events in Caulobacter crescentus, including the initiation of DNA replication, DNA methylation, cell division, and flagellar biogenesis. CtrA is a member of the response regulator family of two component signal transduction systems. Caulobacter goes to great lengths to control the time and place of the activity of this critical regulatory factor during the cell cycle. These controls include temporally regulated transcription and phosphorylation and spatially restricted proteolysis. We report here that ctrA expression is under the control of two promoters: a promoter (P1) that is active only in the early predivisional cell and a stronger promoter (P2) that is active in the late predivisional cell. Both promoters exhibit CtrA-mediated feedback regulation: the early P1 promoter is negatively controlled by CtrA, and the late P2 promoter is under positive feedback control. The CtrA protein footprints conserved binding sites within the P1 and P2 promoters. We propose that the P1 promoter is activated after the initiation of DNA replication in the early predivisional cell. The ensuing accumulation of CtrA results in the activation of the P2 promoter and the repression of the P1 promoter late in the cell cycle. Thus, two transcriptional feedback loops coupled to cell cycle-regulated proteolysis and phosphorylation of the CtrA protein result in the pattern of CtrA activity required for the temporal and spatial control of multiple cell-cycle events.  (+info)

Regulation of podJ expression during the Caulobacter crescentus cell cycle. (7/444)

The polar organelle development gene, podJ, is expressed during the swarmer-to-stalked cell transition of the Caulobacter crescentus cell cycle. Mutants with insertions that inactivate the podJ gene are nonchemotactic, deficient in rosette formation, and resistant to polar bacteriophage, but they divide normally. In contrast, hyperexpression of podJ results in a lethal cell division defect. Nucleotide sequence analysis of the podJ promoter region revealed a binding site for the global response regulator, CtrA. Deletion of this site results in increased overall promoter activity, suggesting that CtrA is a negative regulator of the podJ promoter. Furthermore, synchronization studies have indicated that temporal regulation is not dependent on the presence of the CtrA binding site. Thus, although the level of podJ promoter activity is dependent on the CtrA binding site, the temporal control of podJ promoter expression is dependent on other factors.  (+info)

Bacterial cells: The migrating kinase and the master regulator. (8/444)

It is becoming clear that, as in eukaryotes, proteins in bacterial cells are targeted to specific cellular locations. The most recently discovered example is a remarkable histidine kinase that oscillates between polar and global distributions while temporally regulating transcription and DNA replication in Caulobacter.  (+info)

Wikimedia Commons has media related to Caulobacter crescentus. Caulobacter crescentus Bacterium makes nature's strongest glue ( ... C. crescentus is synonymous with Caulobacter vibrioides. The Caulobacter CB15 genome has 4,016,942 base pairs in a single ... Caulobacter crescentus is a member of a group of bacteria that possess the stalk structure, a tubular extension from the cell ... Caulobacter crescentus is a Gram-negative, oligotrophic bacterium widely distributed in fresh water lakes and streams. The ...
... (Caulobacter response to famine RNA) is a family of non-coding RNAs found in Caulobacter crescentus. CrfA is expressed ... Landt SG, Abeliuk E, McGrath PT, Lesley JA, McAdams HH, Shapiro L (May 2008). "Small non-coding RNAs in Caulobacter crescentus ... Hinz AJ, Larson DE, Smith CS, Brun YV (February 2003). "The Caulobacter crescentus polar organelle development protein PodJ is ... Ely B (1991). "Genetics of Caulobacter crescentus". Meth. Enzymol. 204: 372-384. doi:10.1016/0076-6879(91)04019-K. PMID 1658564 ...
Hardwick SW, Chan VS, Broadhurst RW, Luisi BF (March 2011). "An RNA degradosome assembly in Caulobacter crescentus". Nucleic ... crescentus and B. subtilis degradosomes respectively. The reason for the presence of these enzymes is currently unclear. This ...
2001). "Complete genome sequence of Caulobacter crescentus". Proc. Natl. Acad. Sci. U.S.A. 98 (7): 4136-41. Bibcode:2001PNAS... ...
nov., a novel predator of Caulobacter crescentus". International Journal of Systematic and Evolutionary Microbiology. 63 (1): ...
Bacillus subtilis Caulobacter crescentus Madigan, Michael T.; Martinko, John M. (2006). Brock Biology of Microorganisms (11th ...
Braz VS, Marques MV (October 2005). "Genes involved in cadmium resistance in Caulobacter crescentus". FEMS Microbiology Letters ...
A well established example of bacterial aging is Caulobacter crescentus. This bacteria begins its life as a motile swarmer cell ... such as Caulobacter crescentus, show signs of replicative aging. The results for symmetrically dividing bacteria are more ...
This was verified with the resolution of the crystal structure of the DGC PleD from Caulobacter crescentus in complex with c-di ... The GGDEF domain was first identified in the regulatory protein, PleD of the bacterium Caulobacter crescentus. It was later ... In the cell cycle of Caulobacter crescentus, DGC PleD is known to control pole morphogenesis. In Pseudomonas fluorescens DGC ... Skerker JM, Laub MT (April 2004). "Cell-cycle progression and the generation of asymmetry in Caulobacter crescentus". Nature ...
The bacterium Caulobacter crescentus contains a third protein, crescentin, that is related to the intermediate filaments of ... "Multiple large filament bundles observed in Caulobacter crescentus by electron cryotomography". Molecular Microbiology. 62 (1 ...
Nguyen, Doreen; Ely, Bert (June 2018). "A Genome Comparison of T7-like Podoviruses That Infect Caulobacter crescentus". Current ... a group of giant viruses that appear to be Caulobacter specific. Viruses in Podoviridae are non-enveloped, with icosahedral and ...
As a postdoc she developed photoactivated localization microscopy systems to image Caulobacter crescentus, acquiring the first ... "Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP". Nature Methods. 5 (11): 947-949. doi ...
With the model bacterium Caulobacter crescentus, Jenal discovered that c-di-GMP controls the transition from motile bacteria to ... "Signal transduction mechanisms in Caulobacter crescentus development and cell cycle control". FEMS Microbiol. Rev. 24 (2): 177- ... "Precise timing of transcription by c-di-GMP coordinates cell cycle and morphogenesis in Caulobacter". Nature Communications. 12 ...
More work can be found that focus on modeling a particular cellular process such as the growth cycle of Caulobacter crescentus ... "Temporal Controls of the Asymmetric Cell Division Cycle in Caulobacter crescentus". PLOS Comput Biol. 5 (8): e1000463. Bibcode: ...
In Caulobacter crescentus Ccrm is produced at the end of the replication cycle when Ccrm recognition sites are hemimethylated, ... CcrM role have been characterized in the marine model organism Caulobacter crescentus, which is suitable for the study of cell ... Albu, R. F.; Jurkowski, T. P.; Jeltsch, A. (2012-02-01). "The Caulobacter crescentus DNA-(adenine-N6)-methyltransferase CcrM ... Woodcock, Clayton B.; Yakubov, Aziz B.; Reich, Norbert O. (August 2017). "Caulobacter crescentus Cell Cycle-Regulated DNA ...
Its best-known member is Caulobacter crescentus, an organism ubiquitous in freshwater lakes and rivers; many members of the ... December 2015). "Recurrent Peritonitis Due to Caulobacter crescentus as a Rare Cause of Dialysis Technique Failure". Peritoneal ... Although Caulobacter is not commonly appreciated as a cause of human diseases, Caulobacter isolates have been implicated in a ... One study has identified the species C. crescentus and C. mirare as the cause of a disease of the moth Galleria mellonella; the ...
"SpoT regulates DnaA stability and initiation of DNA replication in carbon-starved Caulobacter crescentus". Journal of ...
socAB is a type VI toxin-antitoxin system that was discovered in Caulobacter crescentus. The antitoxin, SocA, promotes ...
Main lab strain is '168'. Caulobacter crescentus, bacterium that divides into two distinct cells used to study cellular ...
... biprosthecum is a stalked bacterial species phylogenetically closely related to the species Caulobacter crescentus. However, ... Poindexter, JS (Sep 1964). "BIOLOGICAL PROPERTIES AND CLASSIFICATION OF THE CAULOBACTER GROUP". Microbiol. Mol. Biol. Rev. 28 ( ...
EcoDam from E. coli and CcrM from Caulobacter crescentus are well characterized members of these family. More recently, CamA ...
... superresolution colocalization of intracellular protein superstructures and the cell surface in live Caulobacter crescentus". ...
In the less common type, such as the Bacillus subtilis sporulation factor Spo0B or the Caulobacter crescentus protein ChpT, the ... as in the case of the Caulobacter crescentus ChpT HPt involved in cell cycle regulation, or, alternatively, pathways in which ... an essential regulator of stalk biogenesis in Caulobacter crescentus". Journal of Molecular Biology. 390 (4): 686-98. doi: ...
"Two RND proteins involved in heavy metal efflux in Caulobacter crescentus belong to separate clusters within proteobacteria". ...
... is required for proper cytokinesis in bacteria such as Escherichia coli, Caulobacter crescentus, and Bacillus subtilis. ...
This type of regulation seems to occur in other species such as Bacillus subtilis and Caulobacter crescentus. However, other ...
Laccases are also widely distributed among bacterial species, including Bacillus subtilis, Caulobacter crescentus, Escherichia ...
... concave side of the crescent-shaped bacterium Caulobacter crescentus. Both MreB and crescentin are necessary for C. crescentus ... MreB condenses from its normal helical network and forms a tight ring at the septum in Caulobacter crescentus right before cell ... Several rod shaped species, including Escherichia coli and Caulobacter crescentus, use one or more inhibitors of FtsZ assembly ... stalk formation by Caulobacter crescentus, and helical shape of Helicobacter pylori. Within the phylum Spirochaetes, a number ...
Caulobacter crescentus, and began attempting to identify the specific biological processes controlling the cell's cycle. What ... The process of the Caulobacter cell cycle also show similarities to stem cell division, in which two distinct cells arise, one ... In each cell cycle, Caulobacter divides asymmetrically into two daughters. One, the swarmer cell, has a tail-like flagellum ... Examining the cell cycle control logic of Caulobacter as a state machine leads to understanding of bacterial cell cycle ...
In 1996, she was awarded a Ph.D. from Stanford University in Developmental Biology for her studies on Caulobacter crescentus ...
Scientists have developed and explored a variety of scientific models, from Lucy Shapiro's single-celled Caulobacter crescentus ...
H7 and Caulobacter crescentus CB15. The sugar is also found in perimycin, an antibiotic produced by the Gram-positive organism ...
Caulobacter crescentus, and Vibrio alginolyticus, the filament is made up of 11 protofilaments approximately parallel to the ...
For a review of c-di-GMP roles in Caulobacter crescentus, Pseudomonas aeruginosa, Komagataeibacter xylinus/​Gluconacetobacter ...
Caulobacter crescentus and B. subtilis cell size is controlled by a simple mechanisms in which cell division occurs after a ...
... a gene encoding a 100 kDa toxin from Bacillus sphaericus SSII-1 and expresson of insecticidal toxins in Caulobacter crescentus ...
3 hemi-methylated DNA to control the life cycle of Caulobacter crescentus and other related species. Distinct from their ... Zweiger G, Marczynski G, Shapiro L (January 1994). "A Caulobacter DNA methyltransferase that functions only in the ...
Regulatory Response to Carbon Starvation in Caulobacter crescentus. PLoS One. Landt S.G., Abeliuk E., McGrath P.T., Lesley J.A ... McAdams H.H., Shapiro L. (2008) Small non-coding RNAs in Caulobacter crescentus. Journal of Molecular Microbiology. Srinivasan ... Goley E.D., Yeh Y.C., Hong S.H., Fero M.J., Abeliuk E., McAdams H.H., and Shapiro L. (2011) Assembly of the Caulobacter cell ... The global regulatory architecture of transcription during the Caulobacter cell cycle., PLoS Genet. Abeliuk E, Christen B, Fero ...
... 1 candidates for acnD: 2-methylcitrate ... catabolism of small carbon sources in Caulobacter crescentus NA1000. *propionate catabolism in Caulobacter crescentus NA1000 ...
Caulobacter crescentus,/i, secretes a sugary substance so sticky that just a tiny bit could hold several cars together. ... First, Caulobacter crescentus attaches to a surface at the end of its cell body, which has a propeller-like flagellum. On ... The tiny water bacterium ,i,Caulobacter crescentus,/i, secretes a sugary substance so sticky that just a tiny bit could hold ... The tiny water bacterium Caulobacter crescentus secretes a sugary substance so sticky that just a tiny bit could withstand the ...
A modular BAM complex in the outer membrane of the alpha-proteobacterium Caulobacter crescentus.. Anwari K, Poggio S, Perry A, ...
Caulobacter crescentus는 호수와 하천에서 발견되는 독특한 박테리아이다. 작은 크기의 원형 염색체와 상대적인 단순성 때문에, 세균이 어떻게 세포 주기를 조절하는지에 대한 모델로 이용되어 왔다. 포스포트랜스퍼 ... Biondi 등은 지금까지 밝혀지지 않았던 ChpT라는 필수 조절인자를 발견하였으며, 이 유전자가 C. crescentus에서 핵심 세포 주기 조절 인자인 CtrA의 기능을 조절한다는 사실을 밝혔다. 이번 연구에서는 주요한 세포 ...
Caulobacter crescentus CB15 Biological Process. none specified Cellular Component. none specified A single slice from the slice ... Caulobacter crescentus CB15 Biological Process. none specified Cellular Component. none specified A single slice taken from the ... Caulobacter crescentus CB15 Biological Process. none specified Cellular Component. none specified A maximum intensity ... slice reconstructed volume of a Caulobacter Crescentus specimen. This particular slice is taken from Z slice # 142. This image ...
Bacteria Caulobacter crescentus. Reference. Gan L, Chen S, Jensen GJ. Molecular organization of Gram-negative peptidoglycan. ...
Live-Cell Fluorescence Imaging of RecN in Caulobacter crescentus Under DNA Damage.. Methods Mol Biol. 2004:239-250.*PubMed ...
Figure 3. Cell division in Caulobacter cres-centus, FtsZ ring formation. Screenshot from Source ...
Characterization of the Caulobacter crescentus holdfast polysaccharide biosynthesis pathway reveals significant redundancy in ...
Caulobacter crescentus Preferred Term Term UI T050688. Date01/01/1999. LexicalTag NON. ThesaurusID NLM (1992). ... Caulobacter crescentus Preferred Concept UI. M0025765. Registry Number. txid155892. Scope Note. A species of gram-negative, ... Caulobacter crescentus. Tree Number(s). B03.440.400.280.280. B03.440.400.425.288.100.100. B03.660.050.090.100.100. Unique ID. ... Caulobacter vibrioides Term UI T000990515. Date10/01/2019. LexicalTag NON. ThesaurusID NLM (2020). ...
Caulobacter B03.440.400.280.280 Caulobacter crescentus B03.440.400.400 Gallionellaceae B03.440.400.425 Gram-Negative Aerobic ... Caulobacter B03.440.400.425.288.100.100 Caulobacter crescentus B03.440.400.425.293 Comamonadaceae B03.440.400.425.293.150 ... Caulobacter B03.660.050.090.100.100 Caulobacter crescentus B03.660.050.340 Holosporaceae B03.660.050.350 Hyphomicrobiaceae ...
Effect of viscoelastic media on caulobacter crescentus swimming speed Seismic velocity structure of the crust and upper mantle ...
... displacement of protein aggregates along the cell length ensures partitioning to both daughter cells in Caulobacter crescentus ...
Electronic microscopy image of Caulobacter crescentus showing the two dissimilar daughter cells during a the cellular division ... the glycosylation enzyme and sugar producing enzymes from Caulobacter crescentus. All these elements are required to obtain a ... Among the six flagellins of Caulobacter crescentus, the model bacterium in the two studies, one is the special one serving a ... The UNIGE microbiologists studied the bacterium Caulobacter crescentus. «These bacteria are very interesting for studying ...
Complete genome sequence of Caulobacter crescentus. Proc Natl Acad Sci U S A 2001, 98: 4136-4141. 10.1073/pnas.061029298 ... Example pathway created by PathoLogic for the Caulobacter cresentus PGDB, CauloCyc The enzymes for the quinolinate synthetase, ... For instance, the annotation of CC1617 (a Caulobacter cresentus gene) as guaB (E.C.# 1.1.1.205) is supported by the fact that ... crescentus A list of the specific putative enzymes assigned in CauloCyc. (HTML 31 KB). ...
Flagellar Mutants Have Reduced Pilus Synthesis in Caulobacter crescentus. Ellison, Courtney K; Rusch, Douglas B; Brun, Yves V. ...
... a-proteobacterium Caulobacter crescentus. PLoS One, 5(1), e8619. PMCID: PMC2797634. [HTML] [PDF] ...
... analyzing fluorescent protein fusions to determine the localizations of over 3,250 proteins in Caulobacter crescentus, a unique ...
Caulobacter crescentus - Preferred Concept UI. M0025765. Scope note. A species of gram-negative, aerobic bacteria that consist ...
Caulobacter crescentus Preferred Term Term UI T050688. Date01/01/1999. LexicalTag NON. ThesaurusID NLM (1992). ... Caulobacter crescentus Preferred Concept UI. M0025765. Registry Number. txid155892. Scope Note. A species of gram-negative, ... Caulobacter crescentus. Tree Number(s). B03.440.400.280.280. B03.440.400.425.288.100.100. B03.660.050.090.100.100. Unique ID. ... Caulobacter vibrioides Term UI T000990515. Date10/01/2019. LexicalTag NON. ThesaurusID NLM (2020). ...
Caulobacter crescentus is a model organism for the study of asymmetric division and cell type differentiation, as its cell ... Although many genes, proteins, and other molecules involved in the asymmetric division exhibited by C. Crescentus have been ... crescentus. These models provide useful tools for the characterization and analysis of other complex biological networks. ...
We measured the distance between fluorescent-labeled DNA loci of various interloci contour lengths in Caulobacter crescentus ... Caulobacter chromosome in vivo configuration matches model predictions for a supercoiled polymer in a cell-like confinement ...
The oxidative D-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram- ... The oxidative D-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram- ...
Caulobacter crescentus CB15 CC0762. cydA. -93. 6.2. GCCTTGATCCGGATCAAGGA. CC3407. ctaC. -45. 5.6. GCCTTGATCGGGCTCAAATC. ...
Lucy Shapiro employs the bacterial model system Caulobacter crescentus to probe fundamental aspects of developmental biology. ... Shapiros research aims to define the complete genetic circuitry linking cell specification and the cell cycle in Caulobacter. ... Caulobacter cell division. Dr. Shapiros studies have revealed a striking similarity between the organization of cell cycle ...
Pili (green) on cells from the bacterium Caulobacter crescentus (orange). Scientists used a fluorescent dye to stain pili so ... Working with the bacterium Caulobacter crescentus, Indiana University Ph.D. student Courtney Ellison and her colleagues, under ...
... and the bacterium Caulobacter crescentus has led to important insights on the regulation of cell division. ...
Characterization of the Proteins Associated with Caulobacter crescentus Bacteriophage CbK Particles. Callahan Courtney T, ...
Transcriptional profiling of Caulobacter crescentus during growth on complex and minimal media. J Bacteriol. 2004;186:1448-61. ...
  • The tiny water bacterium Caulobacter crescentus secretes a sugary substance so sticky that just a tiny bit could withstand the pull from lifting several cars at once. (livescience.com)
  • Among the six flagellins of C aulobacter crescentus , the model bacterium in the two studies, one is the special one serving a signalling role to trigger the final assembly of the flagellum. (unige.ch)
  • In a Caulobacter crescentus bacterium, PopZ accumulates at one end of the cell. (rupress.org)
  • The oxidative D-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. (tno.nl)
  • The fungus Neurospora crassa has been an important player in understanding the genetics of circadian rhythms, and the bacterium Caulobacter crescentus has led to important insights on the regulation of cell division. (nih.gov)
  • To guarantee that PopZ amasses at the new pole, Caulobacter takes advantage of the protein ParA, which is part of the machinery that separates the chromosomes. (rupress.org)
  • We further comment on how these molecular differences impact bacterial motility and highlight how no single flagellin filament achieves wild-type levels of motility, suggesting C. crescentus has evolved to produce a filament optimized for motility comprised of six flagellins. (bvsalud.org)
  • 6. Molecular recognition of RhlB and RNase D in the Caulobacter crescentus RNA degradosome. (nih.gov)
  • "Growth-driven displacement of protein aggregates along the cell length ensures partitioning to both daughter cells in Caulobacter crescentus," Molecular Microbiology , vol. 111, no. 6, pp. 1430-1448, 2019. (kth.se)
  • Although many genes, proteins, and other molecules involved in the asymmetric division exhibited by C. Crescentus have been discovered and characterized during decades, it remains as a challenging task to understand how cell properties arise from the high number of interactions between these molecular components. (ginsim.org)
  • In particular, she and her colleagues have embraced the power of genomics to identify and understand the molecular circuitry underlying Caulobacter cell division. (nih.gov)
  • Here we present an in-depth analysis of several single flagellin filaments from C. crescentus, including an extremely well-resolved structure of a bacterial flagellar filament. (bvsalud.org)
  • Dr. Lucy Shapiro employs the bacterial model system Caulobacter crescentus to probe fundamental aspects of developmental biology. (nih.gov)
  • For instance, the annotation of CC1617 (a Caulobacter cresentus gene) as guaB (E.C.# 1.1.1.205) is supported by the fact that CC1617 is part of a predicted operon with guaA (E.C.# 6.3.5.2) and the pathway for de novo biosynthesis of purine nucleotides (I) includes both of these reactions. (biomedcentral.com)
  • Characterization of the Proteins Associated with Caulobacter crescentus Bacteriophage CbK Particles. (cdc.gov)
  • Atomic-level architecture of Caulobacter crescentus flagellar filaments provide evidence for multi-flagellin filament stabilization. (bvsalud.org)
  • This is the case for Caulobacter crescentus , but little is known about why this species encodes six different flagellin genes . (bvsalud.org)
  • First, Caulobacter crescentus attaches to a surface at the end of its cell body, which has a propeller-like flagellum. (livescience.com)
  • Live-Cell Fluorescence Imaging of RecN in Caulobacter crescentus Under DNA Damage. (ncbs.res.in)
  • It was a vi-su-al jour-ney in-to and through the in-ter-ior of a cell, and en-ti-re-ly bas-ed on the re-sults de-scrib-ed in their pa-per (Fi-gure 1, and Fron-ti-spie-ce). (asmblog.org)
  • Cell division in Caulobacter cres-centus , FtsZ ring formation. (asmblog.org)
  • Caulobacter crescentus is a model organism for the study of asymmetric division and cell type differentiation, as its cell division cycle generates a pair of daughter cells that differ from one another in their morphology and behavior. (ginsim.org)
  • Dr. Shapiro's research aims to define the complete genetic circuitry linking cell specification and the cell cycle in Caulobacter . (nih.gov)
  • for ex-ample, vir-tu-al-ly 'carv-ed-out sten-cils' from crys-tal da-ta in the PDB col-lect-ion to un-mis-tak-ab-ly pin-point ri-bo-som-es in-clud-ing their ori-en-ta-tion in elec-tron cryo-to-mo-grams of E. coli cells (see here in STC. (asmblog.org)
  • To figure out the binding process in Caulobacter crescentus , researchers from Indiana University and Brown University used high-resolution video microscopy to watch it progress in real time. (livescience.com)
  • Dr. Lucy Shapiro employs the bacterial model system Caulobacter crescentus to probe fundamental aspects of developmental biology. (nih.gov)