Caulobacter crescentus. Medical search

A species of gram-negative, aerobic bacteria that consist of slender vibroid cells.

A genus of gram-negative, aerobic, rod- or vibroid-shaped or fusiform bacteria that commonly produce a stalk. They are found in fresh water and soil and divide by binary transverse fission.

A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)

Proteins found in any species of bacterium.

One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.

Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.

A protein with a molecular weight of 40,000 isolated from bacterial flagella. At appropriate pH and salt concentration, three flagellin monomers can spontaneously reaggregate to form structures which appear identical to intact flagella.

The functional hereditary units of BACTERIA.

The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.

Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.

A class in the phylum PROTEOBACTERIA comprised mostly of two major phenotypes: purple non-sulfur bacteria and aerobic bacteriochlorophyll-containing bacteria.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

Physiological processes and properties of BACTERIA.

Deoxyribonucleic acid that makes up the genetic material of bacteria.

An enzyme responsible for producing a species-characteristic methylation pattern on adenine residues in a specific short base sequence in the host cell DNA. The enzyme catalyzes the methylation of DNA adenine in the presence of S-adenosyl-L-methionine to form DNA containing 6-methylaminopurine and S-adenosyl-L-homocysteine. EC 2.1.1.72.

A large group of aerobic bacteria which show up as pink (negative) when treated by the gram-staining method. This is because the cell walls of gram-negative bacteria are low in peptidoglycan and thus have low affinity for violet stain and high affinity for the pink dye safranine.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

The process by which a DNA molecule is duplicated.

The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.

In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.

A DNA-directed RNA polymerase found in BACTERIA. It is a holoenzyme that consists of multiple subunits including sigma factor 54.

Viruses whose hosts are bacterial cells.

An ATP-dependent protease found in prokaryotes, CHLOROPLASTS, and MITOCHONDRIA. It is a soluble multisubunit complex that plays a role in the degradation of many abnormal proteins.

Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.

Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.

A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.

A potassium salt used to replenish ELECTROLYTES, for restoration of WATER-ELECTROLYTE BALANCE, as well as a urinary and systemic alkalizer, which can be administered orally or by intravenous infusion. Formerly, it was used in DIURETICS and EXPECTORANTS.

DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.

The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.

Tungsten hydroxide oxide phosphate. A white or slightly yellowish-green, slightly efflorescent crystal or crystalline powder. It is used as a reagent for alkaloids and many other nitrogen bases, for phenols, albumin, peptone, amino acids, uric acid, urea, blood, and carbohydrates. (From Merck Index, 11th ed)

A flavoring agent. It is the intermediate product in the two-step bioconversion of ferulic acid to vanillin. (J Biotechnol 1996;50(2-3):107-13).

A unique DNA sequence of a replicon at which DNA REPLICATION is initiated and proceeds bidirectionally or unidirectionally. It contains the sites where the first separation of the complementary strands occurs, a primer RNA is synthesized, and the switch from primer RNA to DNA synthesis takes place. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)

Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.

A protein which is a subunit of RNA polymerase. It effects initiation of specific RNA chains from DNA.

D-Galactose:NAD(P)+ 1-oxidoreductases. Catalyzes the oxidation of D-galactose in the presence of NAD+ or NADP+ to D-galactono-gamma-lactone and NADH or NADPH. Includes EC 1.1.1.48 and EC 1.1.1.120.

Enzymes that catalyze the cleavage of a phosphorus-oxygen bond by means other than hydrolysis or oxidation. EC 4.6.

The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.

Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.

Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.

A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.

Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.

Proteins isolated from the outer membrane of Gram-negative bacteria.

Porins are protein molecules that were originally found in the outer membrane of GRAM-NEGATIVE BACTERIA and that form multi-meric channels for the passive DIFFUSION of WATER; IONS; or other small molecules. Porins are present in bacterial CELL WALLS, as well as in plant, fungal, mammalian and other vertebrate CELL MEMBRANES and MITOCHONDRIAL MEMBRANES.

The etiologic agent of CHOLERA.

The genetic complement of a BACTERIA as represented in its DNA.

Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)

The science that investigates the principles governing correct or reliable inference and deals with the canons and criteria of validity in thought and demonstration. This system of reasoning is applicable to any branch of knowledge or study. (Random House Unabridged Dictionary, 2d ed & Sippl, Computer Dictionary, 4th ed)

Chromosome methylation and measurement of faithful, once and only once per cell cycle chromosome replication in Caulobacter crescentus. (1/444)

Caulobacter crescentus exhibits cell-type-specific control of chromosome replication and DNA methylation. Asymmetric cell division yields a replicating stalked cell and a nonreplicating swarmer cell. The motile swarmer cell must differentiate into a sessile stalked cell in order to replicate and execute asymmetric cell division. This program of cell division implies that chromosome replication initiates in the stalked cell only once per cell cycle. DNA methylation is restricted to the predivisional cell stage, and since DNA synthesis produces an unmethylated nascent strand, late DNA methylation also implies that DNA near the replication origin remains hemimethylated longer than DNA located further away. In this report, both assumptions are tested with an engineered Tn5-based transposon, Tn5Omega-MP. This allows a sensitive Southern blot assay that measures fully methylated, hemimethylated, and unmethylated DNA duplexes. Tn5Omega-MP was placed at 11 sites around the chromosome and it was clearly demonstrated that Tn5Omega-MP DNA near the replication origin remained hemimethylated longer than DNA located further away. One Tn5Omega-MP placed near the replication origin revealed small but detectable amounts of unmethylated duplex DNA in replicating stalked cells. Extra DNA synthesis produces a second unmethylated nascent strand. Therefore, measurement of unmethylated DNA is a critical test of the "once and only once per cell cycle" rule of chromosome replication in C. crescentus. Fewer than 1 in 1,000 stalked cells prematurely initiate a second round of chromosome replication. The implications for very precise negative control of chromosome replication are discussed with respect to the bacterial cell cycle. (+info)

The CtrA response regulator mediates temporal control of gene expression during the Caulobacter cell cycle. (2/444)

In its role as a global response regulator, CtrA controls the transcription of a diverse group of genes at different times in the Caulobacter crescentus cell cycle. To understand the differential regulation of CtrA-controlled genes, we compared the expression of two of these genes, the fliQ flagellar gene and the ccrM DNA methyltransferase gene. Despite their similar promoter architecture, these genes are transcribed at different times in the cell cycle. PfliQ is activated earlier than PccrM. Phosphorylated CtrA (CtrA approximately P) bound to the CtrA recognition sequence in both promoters but had a 10- to 20-fold greater affinity for PfliQ. This difference in affinity correlates with temporal changes in the cellular levels of CtrA. Disrupting a unique inverted repeat element in PccrM significantly reduced promoter activity but not the timing of transcription initiation, suggesting that the inverted repeat does not play a major role in the temporal control of ccrM expression. Our data indicate that differences in the affinity of CtrA approximately P for PfliQ and PccrM regulate, in part, the temporal expression of these genes. However, the timing of fliQ transcription but not of ccrM transcription was altered in cells expressing a stable CtrA derivative, indicating that changes in CtrA approximately P levels alone cannot govern the cell cycle transcription of these genes. We propose that changes in the cellular concentration of CtrA approximately P and its interaction with accessory proteins influence the temporal expression of fliQ, ccrM, and other key cell cycle genes and ultimately the regulation of the cell cycle. (+info)

Cell cycle-dependent polar localization of an essential bacterial histidine kinase that controls DNA replication and cell division. (3/444)

The master CtrA response regulator functions in Caulobacter to repress replication initiation in different phases of the cell cycle. Here, we identify an essential histidine kinase, CckA, that is responsible for CtrA activation by phosphorylation. Although CckA is present throughout the cell cycle, it moves to a cell pole in S phase, and upon cell division it disperses. Removal of the membrane-spanning region of CckA results in loss of polar localization and cell death. We propose that polar CckA functions to activate CtrA just after the initiation of DNA replication, thereby preventing premature reinitiations of chromosome replication. Thus, dynamic changes in cellular location of critical signal proteins provide a novel mechanism for the control of the prokaryote cell cycle. (+info)

Identification and transcriptional control of the genes encoding the Caulobacter crescentus ClpXP protease. (4/444)

The region of the Caulobacter crescentus chromosome harboring the genes for the ClpXP protease was isolated and characterized. Comparison of the deduced amino acid sequences of the C. crescentus ClpP and ClpX proteins with those of their homologues from several gram-positive and gram-negative bacteria revealed stronger conservation for the ATPase regulatory subunit (ClpX) than for the peptidase subunit (ClpP). The C. crescentus clpX gene was shown by complementation analysis to be functional in Escherichia coli. However, clpX from E. coli was not able to substitute for the essential nature of the clpX gene in C. crescentus. The clpP and clpX genes are separated on the C. crescentus chromosome by an open reading frame pointing in the opposite direction from the clp genes, and transcription of clpP and clpX was found to be uncoupled. clpP is transcribed as a monocistronic unit with a promoter (PP1) located immediately upstream of the 5' end of the gene and a terminator structure following its 3' end. PP1 is under heat shock control and is induced upon entry of the cells into the stationary phase. At least three promoters for clpX (PX1, PX2, and PX3) were mapped in the clpP-clpX intergenic region. In contrast to PP1, the clpX promoters were found to be downregulated after heat shock but were also subject to growth phase control. In addition, the clpP and clpX promoters showed different activity patterns during the cell cycle. Together, these results demonstrate that the genes coding for the peptidase and the regulatory subunits of the ClpXP protease are under independent transcriptional control in C. crescentus. Determination of the numbers of ClpP and ClpX molecules per cell suggested that ClpX is the limiting component compared with ClpP. (+info)

Cell cycle expression and transcriptional regulation of DNA topoisomerase IV genes in caulobacter. (5/444)

DNA replication and differentiation are closely coupled during the Caulobacter crescentus cell cycle. We have previously shown that DNA topoisomerase IV (topo IV), which is encoded by the parE and parC genes, is required for chromosomal partitioning, cell division, and differentiation in this bacterium (D. Ward and A. Newton, Mol. Microbiol. 26:897-910, 1997). We have examined the cell cycle regulation of parE and parC and report here that transcription of these topo IV genes is induced during the swarmer-to-stalked-cell transition when cells prepare for initiation of DNA synthesis. The regulation of parE and parC expression is not strictly coordinated, however. The rate of parE transcription increases ca. 20-fold during the G1-to-S-phase transition and in this respect, its pattern of regulation is similar to those of several other genes required for chromosome duplication. Transcription from the parC promoter, by contrast, is induced only two- to threefold during this cell cycle period. Steady-state ParE levels are also regulated, increasing ca. twofold from low levels in swarmer cells to a maximum immediately prior to cell division, while differences in ParC levels during the cell cycle could not be detected. These results suggest that topo IV activity may be regulated primarily through parE expression. The presumptive promoters of the topo IV genes display striking similarities to, as well as differences from, the consensus promoter recognized by the major Caulobacter sigma factor sigma73. We also present evidence that a conserved 8-mer sequence motif located in the spacers between the -10 and -35 elements of the parE and parC promoters is required for maximum levels of parE transcription, which raises the possibility that it may function as a positive regulatory element. The pattern of parE transcription and the parE and parC promoter architecture suggest that the topo IV genes belong to a specialized subset of cell cycle-regulated genes required for chromosome replication. (+info)

Feedback control of a master bacterial cell-cycle regulator. (6/444)

The transcriptional regulator CtrA controls several key cell-cycle events in Caulobacter crescentus, including the initiation of DNA replication, DNA methylation, cell division, and flagellar biogenesis. CtrA is a member of the response regulator family of two component signal transduction systems. Caulobacter goes to great lengths to control the time and place of the activity of this critical regulatory factor during the cell cycle. These controls include temporally regulated transcription and phosphorylation and spatially restricted proteolysis. We report here that ctrA expression is under the control of two promoters: a promoter (P1) that is active only in the early predivisional cell and a stronger promoter (P2) that is active in the late predivisional cell. Both promoters exhibit CtrA-mediated feedback regulation: the early P1 promoter is negatively controlled by CtrA, and the late P2 promoter is under positive feedback control. The CtrA protein footprints conserved binding sites within the P1 and P2 promoters. We propose that the P1 promoter is activated after the initiation of DNA replication in the early predivisional cell. The ensuing accumulation of CtrA results in the activation of the P2 promoter and the repression of the P1 promoter late in the cell cycle. Thus, two transcriptional feedback loops coupled to cell cycle-regulated proteolysis and phosphorylation of the CtrA protein result in the pattern of CtrA activity required for the temporal and spatial control of multiple cell-cycle events. (+info)

Regulation of podJ expression during the Caulobacter crescentus cell cycle. (7/444)

The polar organelle development gene, podJ, is expressed during the swarmer-to-stalked cell transition of the Caulobacter crescentus cell cycle. Mutants with insertions that inactivate the podJ gene are nonchemotactic, deficient in rosette formation, and resistant to polar bacteriophage, but they divide normally. In contrast, hyperexpression of podJ results in a lethal cell division defect. Nucleotide sequence analysis of the podJ promoter region revealed a binding site for the global response regulator, CtrA. Deletion of this site results in increased overall promoter activity, suggesting that CtrA is a negative regulator of the podJ promoter. Furthermore, synchronization studies have indicated that temporal regulation is not dependent on the presence of the CtrA binding site. Thus, although the level of podJ promoter activity is dependent on the CtrA binding site, the temporal control of podJ promoter expression is dependent on other factors. (+info)

Bacterial cells: The migrating kinase and the master regulator. (8/444)

It is becoming clear that, as in eukaryotes, proteins in bacterial cells are targeted to specific cellular locations. The most recently discovered example is a remarkable histidine kinase that oscillates between polar and global distributions while temporally regulating transcription and DNA replication in Caulobacter. (+info)

C. crescentus is synonymous with Caulobacter vibrioides. The Caulobacter CB15 genome has 4,016,942 base pairs in a single ... Caulobacter crescentus is a member of a group of bacteria that possess the stalk structure, a tubular extension from the cell ... Caulobacter crescentus is a Gram-negative, oligotrophic bacterium widely distributed in fresh water lakes and streams. The ... Presumably, It does so by a gain of function after protein expansion from around 400 amino acids in Caulobacter crescentus to ...

https://en.wikipedia.org/wiki/Caulobacter_crescentus

Complete genome sequence of Caulobacter crescentus. William C. Nierman, Tamara V. Feldblyum, Michael T. Laub, Ian T. Paulsen, ... Complete genome sequence of Caulobacter crescentus. William C. Nierman, Tamara V. Feldblyum, Michael T. Laub, Ian T. Paulsen, ... Complete genome sequence of Caulobacter crescentus. William C. Nierman, Tamara V. Feldblyum, Michael T. Laub, Ian T. Paulsen, ... The complete genome sequence of Caulobacter crescentus was determined to be 4,016,942 base pairs in a single circular ...

https://www.pnas.org/content/98/7/4136?ijkey=955325336e3ab1a7c3c3eb0eda4c81cd82d4e432&keytype2=tf_ipsecsha

The evolution of stalk positioning in the Caulobacter cladeEdit. Diverse positioning of the stalks. Caulobacter crescentus ( ... Caulobacter crescentus is a member of a group of bacteria that possess the stalk structure, a tubular extension from the cell ... Caulobacter crescentus is a Gram-negative, oligotrophic bacterium widely distributed in fresh water lakes and streams. The ... Jenal, U (Nov 2009). "The role of proteolysis in the Caulobacter crescentus cell cycle and development". Research in ...

https://en.m.wikipedia.org/wiki/Caulobacter_crescentus

1984) Caulobacter crescentus flagellar filament has a right-handed helical form. J Mol Biol 173(1):125-130. ... Helical motion of the cell body enhances Caulobacter crescentus motility. Bin Liu, Marco Gulino, Michael Morse, Jay X. Tang, ... Helical motion of the cell body enhances Caulobacter crescentus motility Message Subject (Your Name) has sent you a message ... 2006) Low flagellar motor torque and high swimming efficiency of Caulobacter crescentus swarmer cells. Biophys J 91(7):2726- ...

http://www.pnas.org/content/111/31/11252

Walker S.G., Smit J. (1993) Attachment of the S-Layer of Caulobacter crescentus to the Cell Surface. In: Beveridge T.J., Koval ... Smit, J., Grano, D.A., Glaser, R.M., and Agabian, N., 1981, Periodic surface array in Caulobacter crescentus: fine structure ... Gilchrist, A., Fisher, J.A., and Smit, J., 1992, Nucleotide sequence analysis of the gene encoding the Caulobacter crescentus ... Smit, J., Engelhardt, H., Volker, S., Smith, S.H., and Baumeister, W., 1992, The Slayer of Caulobacter crescentus: Three- ...

https://link.springer.com/chapter/10.1007/978-1-4757-9032-0_29

The Caulobacter crescentus flagellar gene, fliX, encodes a novel trans-acting factor that couples flagellar assembly to ... The first flagellar assembly checkpoint of Caulobacter crescentus couples assembly of the early class II components of the ...

https://www.ingentaconnect.com/content/bsc/mole/2001/00000039/00000006/art00019

From left to right: i) The number of proteins in the reference proteome of Caulobacter crescentus, ii) the total number of ... The bar plot shows the coverage for every protein in the reference proteome of Caulobacter crescentus for which there is at ... Caulobacter crescentus is a Gram-negative, oligotrophic bacterium widely distributed in freshwater lakes and streams. ... The plot shows the evolution over years (x-axis) of the fraction of Caulobacter crescentus reference proteome residues (y-axis ...

https://swissmodel.expasy.org/repository/species/190650

... and cell division in stalked cells of the α-proteobacterium Caulobacter crescentus. The model is formulated in terms of ... for the spatial asymmetry of Caulobacter reproduction (swarmer cells as well as stalked cells), the correlation of cell growth ... nonlinear ordinary differential equations for the major cell cycle regulatory proteins in Caulobacter: CtrA, GcrA, DnaA, CcrM, ... Caulobacter crescentus Is the Subject Area "Caulobacter crescentus" applicable to this article? Yes. No. ...

https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.0040009

Apparently, C. crescentus is not well adapted for efficient growth on high xylose levels, and responds by an extended lag phase ... C. crescentus was grown on xylose, arabinose and glucose, and maximum specific growth rates determined for the three substrates ... Caulobacter crescentus is a gram-negative bacterium that can utilize xylose as a substrate using the Weimberg pathway, which ... Caulobacter crescentus; Weimberg pathway; xylose; arabinose; physiological characterization; enzyme activity Caulobacter ...

https://www.mdpi.com/2311-5637/4/2/44

Caulobacter vibrioides CB15. Mutation(s): 0 Gene Names: Itgav, CC_2357. Find proteins for Q9A5U0 (Caulobacter vibrioides ( ... Structure of a GH39 Beta-xylosidase from Caulobacter crescentus. Polo, C.C., Santos, C.R., Correa, J.M., Simao, R.C.G., Seixas ... Structure of a GH39 Beta-xylosidase from Caulobacter crescentus. *DOI: 10.2210/pdb4M29/pdb ...

https://www.rcsb.org/structure/4m29

Caulobacter crescentus in Biotechnology. From MicrobeWiki, the student-edited microbiology resource. Revision as of 05:51, 15 ... Caulobacter crescentus is a non-pathogenic, aquatic, Gram-negative bacterium.[9] One of its most distinguishing characteristics ... C. crescentus and Pseudomonas aeruginosa vaccine candidate. The novel protein secretion system of C. crescentus was used to ... C. crescentus and HIV. The display capabilities of C. crescentus can be used to develop antibodies, which can mimic host ...

https://microbewiki.kenyon.edu/index.php?title=Caulobacter_crescentus_in_Biotechnology&oldid=78931

Nucleotide excision repair - Caulobacter crescentus CB15 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...

http://www.genome.jp/kegg-bin/show_pathway?ccr03420+CC_3610

Caulobacter vibrioides (Caulobacter crescentus). Caulobacter sp. 410. Caulobacter flavus. Caulobacter sp. FWC26. 231. UniRef90_ ... Caulobacter crescentus (strain ATCC 19089 / CB15). 231. UniRef100_B8H627. Cluster: Ribonuclease 3. 2. ... sp,B8H627,RNC_CAUCN Ribonuclease 3 OS=Caulobacter crescentus (strain NA1000 / CB15N) OX=565050 GN=rnc PE=3 SV=1 ... Caulobacter vibrioides › Caulobacter crescentus (strain ATCC 19089 / CB15) ...

http://www.uniprot.org/uniprot/B8H627

... Author(s). Gora, Kasia G. (Kasia Gabriela) ... In this thesis, I use the model system Caulobacter crescentus to examine the regulation of CtrA, the essential transcription ... factor at the core of the Caulobacter cell cycle regulatory network. CtrA regulates the activity of approximately 100 cell ...

https://dspace.mit.edu/handle/1721.1/68426

... small bacterial cells was developed and used to identify chemoreceptor arrays in cryotomograms of intact Caulobacter crescentus ... Location and Architecture of the Caulobacter Crescentus Chemoreceptor Array Mol Microbiol. 2008 Jul;69(1):30-41. doi: 10.1111/j ... The arrays were always found on the convex side of the cell, further demonstrating that Caulobacter cells maintain dorsal/ ... small bacterial cells was developed and used to identify chemoreceptor arrays in cryotomograms of intact Caulobacter crescentus ...

https://pubmed.ncbi.nlm.nih.gov/18363791/?dopt=Abstract

Caulobacter vibrioides (Caulobacter crescentus). Caulobacter sp. 410. Caulobacter flavus. Caulobacter sp. FWC2. Caulobacter sp ... Caulobacter sp. X. Caulobacter sp. FWC26. Caulobacter segnis (strain ATCC 21756 / DSM 7131 / JCM 7823 / NBRC 15250 / LMG 17158 ... Caulobacter crescentus (strain ATCC 19089 / CB15). 358. UniRef100_B8GZ33. Cluster: Modification methylase CcrMI. 2. ... sp,B8GZ33,MTC1_CAUCN Modification methylase CcrMI OS=Caulobacter crescentus (strain NA1000 / CB15N) OX=565050 GN=ccrMIM PE=3 SV ...

http://www.uniprot.org/uniprot/B8GZ33

The Genetic Basis of Laboratory Adaptation in Caulobacter crescentus Melissa E. Marks, Cyd Marie Castro-Rojas, Clotilde Teiling ... The Genetic Basis of Laboratory Adaptation in Caulobacter crescentus Melissa E. Marks, Cyd Marie Castro-Rojas, Clotilde Teiling ... The Genetic Basis of Laboratory Adaptation in Caulobacter crescentus Melissa E. Marks, Cyd Marie Castro-Rojas, Clotilde Teiling ... The Genetic Basis of Laboratory Adaptation in Caulobacter crescentus Message Subject (Your Name) has forwarded a page to you ...

https://jb.asm.org/content/192/14/3678?ijkey=d6839e5e4e8aa09b22ec63c277d1f75195403d66&keytype2=tf_ipsecsha

... of the essential transcriptional regulator CtrA is necessary to drive cell cycle progression in Caulobacter crescentus. At the ... Mutations that alter RcdA surface residues decouple protein localization and CtrA proteolysis in Caulobacter crescentus J Mol ... of the essential transcriptional regulator CtrA is necessary to drive cell cycle progression in Caulobacter crescentus. At the ... We assayed the ability of each RcdA variant to support CtrA proteolysis and polar protein localization in Caulobacter. Deletion ...

https://pubmed.ncbi.nlm.nih.gov/19747489/

ppGpp and Polyphosphate Modulate Cell Cycle Progression in Caulobacter crescentus. Cara C. Boutte, Jonathan T. Henry, Sean ... ppGpp and Polyphosphate Modulate Cell Cycle Progression in Caulobacter crescentus. Cara C. Boutte, Jonathan T. Henry, Sean ... ppGpp and Polyphosphate Modulate Cell Cycle Progression in Caulobacter crescentus. Cara C. Boutte, Jonathan T. Henry, Sean ... Caulobacter crescentus displays a dimorphic life cycle, beginning with a flagellated and chemotactic swarmer cell that neither ...

https://jb.asm.org/content/194/1/28

... Author(s). Chen, Yiyin Erin ... In this work, I use the model organism Caulobacter crescentus to investigate how intracellular asymmetry within the mother cell ... Caulobacter is an alpha-proteobacterium that always divides asymmetrically to generate two daughter cells that are ... The genetic separability of the spatial and temporal controls of replication in Caulobacter suggests that DnaA comprises an ...

https://dspace.mit.edu/handle/1721.1/68425

... from the cytoplasm to the cell wall to regulate key developmental shape changes in the α-proteobacterium Caulobacter crescentus ... Caulobacter crescentus Is the Subject Area "Caulobacter crescentus" applicable to this article? Yes. No. ...

https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1006999

An image of slice 210 from the reconstructed volume of a tomographic data set from Caulobacter crescentus. This image has been ... An image of slice 210 from the reconstructed volume of a tomographic data set from Caulobacter crescentus. This image has been ... Caulobacter cultures (strain 3724) were cryofixed using a cocktail of 2% Osmium tetroxide, 0.5% uranyl acetate in anhydrous ... Lucy Shapiro, Harley McAdams (2012) CIL:40007, Caulobacter crescentus CB15. CIL. Dataset. https://doi.org/doi:10.7295/ ...

http://cellimagelibrary.org/images/40007

What is Caulobacter crescentus? Meaning of Caulobacter crescentus medical term. What does Caulobacter crescentus mean? ... Looking for online definition of Caulobacter crescentus in the Medical Dictionary? Caulobacter crescentus explanation free. ... Caulobacter crescentus. Also found in: Encyclopedia, Wikipedia. Caulobacter crescentus. (kawl-ŏ-băk′tĕr krĕ-sĕn′tŭs) A single- ... Caulobacter crescentus , definition of Caulobacter crescentus by Medical dictionary https://medical-dictionary. ...

https://medical-dictionary.thefreedictionary.com/Caulobacter+crescentus

... from Caulobacter crescentus CB15 at 2.83 A resolution (PSI Community Target, Shapiro) ... Caulobacter crescentus (strain ATCC 19089 / CB15). N/A. Find proteins for Q9A712 (Caulobacter crescentus (strain ATCC 19089 / ... Crystal structure of a putative outer membrane chaperone (OmpH-like) (CC_1914) from Caulobacter crescentus CB15 at 2.83 A ... Crystal structure of a putative outer membrane chaperone (OmpH-like) (CC_1914) from Caulobacter crescentus CB15 at 2.83 A ...

https://www.rcsb.org/structure/4kqt

Physiochemical properties of Caulobacter crescentus holdfast: a localized bacterial adhesive. J Phys Chem B 117:10492-10503. ... In Caulobacter crescentus, c-di-GMP regulates the synthesis of the polar holdfast adhesin during the cell cycle, yet the ... We use Caulobacter crescentus as a model to study the regulatory mechanisms of the motile-to-sessile transition of bacteria. ... The HfaB and HfaD adhesion proteins of Caulobacter crescentus are localized in the stalk. Mol Microbiol 49:1671-1683. doi: ...

https://mbio.asm.org/content/8/2/e00294-17

PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in live Caulobacter crescentus. We ... Title High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization ... High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization ... PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in live Caulobacter crescentus. We ...

https://infoscience.epfl.ch/record/198604

iv) In their interspecies killing assays with Caulobacter crescentus Cdz producers, the authors have used two closely related α ... A C. crescentus bacteriocin gene cluster is induced in stationary phase. We hypothesized that, if they exist, Caulobacter genes ... Unlike in Caulobacter crescentus, most of these putative contact-dependent systems were predicted to encode a single Cdz-like ... 2010) A modular BAM complex in the outer membrane of the alpha-proteobacterium caulobacter crescentus PLoS One 5:e8619. ...

https://elifesciences.org/articles/24869

The Caulobacter crescentus GTPase CgtA C is required for progression through the cell cycle and for maintaining 50S ribosomal ... Citation: Datta, Kaustuv; Skidmore, Jennifer M . ; Pu, Kun; Maddock, Janine R . (2004). "The Caulobacter crescentus GTPase CgtA ... we generated mutations in the Caulobacter crescentus obg gene ( cgtA C ) which, in Ras-like proteins, would result in either ... In C. crescentus , a P168V mutant is not activating in vivo , although in vitro , the P168V protein showed a modest reduction ...

https://deepblue.lib.umich.edu/handle/2027.42/75652

Dynamics and control of biofilms of the oligotrophic bacterium Caulobacter crescentus.. Title. Dynamics and control of biofilms ... Caulobacter crescentus is an oligotrophic alpha-proteobacterium with a complex cell cycle involving sessile-stalked and ... Bacterial Adhesion, Biofilms, Caulobacter crescentus, Culture Media, Fimbriae, Bacterial, Flagella, Fresh Water, Gene ... The involvement of pili in mushroom architecture is a novel function for type IV pili in C. crescentus. These unique biofilm ...

http://web.stanford.edu/group/spormannlab/cgi-bin/amslab/content/dynamics-and-control-biofilms-oligotrophic-bacterium-caulobacter-crescentus

We describe the identification of 27 novel Caulobacter crescentus sRNAs by analysis of RNA expression levels assayed using a ... tiled Caulobacter microarray and a protocol optimized for detection of sRNAs. The principal analysis method involved ... Small non-coding RNAs in Caulobacter crescentus.. @article{Landt2008SmallNR, title={Small non-coding RNAs in Caulobacter ... Iron Deficiency Generates Oxidative Stress and Activation of the SOS Response in Caulobacter crescentus. *Laura Leaden, Larissa ...

https://www.semanticscholar.org/paper/Small-non-coding-RNAs-in-Caulobacter-crescentus.-Landt-Abeliuk/9649d19a040fe10da2dab7a9866caba6557396cd

In the laboratory, researchers distinguish between C. crescentus strain CB15 (the strain originally isolated from a freshwater lake) and NA1000 (the primary experimental strain). (wikipedia.org)
The Caulobacter CB15 genome has 4,016,942 base pairs in a single circular chromosome encoding 3,767 genes. (wikipedia.org)
In 2010, the Caulobacter NA1000 strain was sequenced and all differences with the CB15 "wild type" strain were identified. (wikipedia.org)
The genetic basis of phenotypic differences in growth rate, mucoidy, adhesion, sedimentation, phage susceptibility, and stationary-phase survival between C. crescentus strain CB15 and its derivative NA1000 is determined by coding, regulatory, and insertion/deletion polymorphisms at five chromosomal loci. (asm.org)
In an effort to comprehensively characterize their divergence, we identified the genetic basis of all known phenotypic differences between two laboratory strains (NA1000 and CB15) of C. crescentus . (asm.org)
CIL:40007, Caulobacter crescentus CB15. (cellimagelibrary.org)
Lucy Shapiro, Harley McAdams (2012) CIL:40007, Caulobacter crescentus CB15. (cellimagelibrary.org)
Copper-Zinc superoxide dismutase from Caulobacter crescentus CB15. (thefreedictionary.com)
In contrast to biofilms of the well-studied Pseudomonas aeruginosa, Escherichia coli, and Vibrio cholerae, C. crescentus CB15 cells form biphasic biofilms, consisting predominantly of a cell monolayer biofilm and a biofilm containing densely packed, mushroom-shaped structures. (stanford.edu)

Due to this capacity to be physically synchronized, strain NA1000 has become the predominant experimental Caulobacter strain throughout the world. (wikipedia.org)
Comparing the transcriptional profiles of a Caulobacter crescentus CB15N (NA1000) wild-type to a SpoT null in carbon starvation. (omicsdi.org)
The S-layer was extracted from the cell surface of C. crescentus NA1000 by treating cells with a pH 2 solution or a solution containing 10 mM ethylene glycol-bis(ß-aminoethylether)- N,N,N',N'-tetraacetic acid (EGTA). (ubc.ca)
Two mutants of C. crescentus NA1000, JS1001 and JS1002, selected for the ability to grow in the absence of calcium had the additional phenotype of being unable to attach the S-layer to the cell surface although they produced a wild-type RsaA. (ubc.ca)
Caulobacter crescentus NA1000 possess two HME-RND proteins, and the aim of this work was to determine their involvement in the response to cadmium, zinc, cobalt and nickel, and to analyze the phylogenetic distribution and characteristic signatures of orthologs of these two proteins. (beds.ac.uk)

Using a digital tracking microscope that provides both cell position and orientation, we have correlated the detailed motion of the cell body of a fast-swimming bacterium, Caulobacter crescentus , with its swimming motility. (pnas.org)
We have used this technique to study the motility of the uniflagellated bacterium Caulobacter crescentus and have found that each cell displays two distinct modes of motility, depending on the sense of rotation of the flagellar motor. (pnas.org)
The dimorphic bacterium Caulobacter crescentus has evolved marked phenotypic changes during its 50-year history of culture in the laboratory environment, providing an excellent system for the study of natural selection and phenotypic microevolution in prokaryotes. (asm.org)
Likewise, the aquatic bacterium Caulobacter crescentus has evolved marked phenotypic changes during the 50 years it has been cultured in the laboratory environment. (asm.org)
The researchers started the project by trying to understand why the bacterium Caulobacter crescentus produces two distinct types of cell - one with a flagellum (appendage) and the other without. (thefreedictionary.com)
Dynamics and control of biofilms of the oligotrophic bacterium Caulobacter crescentus. (stanford.edu)
However, even that does not compare to what the bacterium Caulobacter crescentus can do. (listverse.com)
A protein critical for cell constriction in the Gram-negative bacterium Caulobacter crescentus localizes at the division site through its peptidoglycan-binding LysM domains. (helmholtz-hzi.de)
In order to gain insight about the material properties of bacterial adhesins, we study the morphogenesis of the adhesive holdfast of the Gram negative bacterium Caulobacter crescentus . (biomedcentral.com)
Microarray analysis was used to examine gene expression in the freshwater oligotrophic bacterium Caulobacter crescentus during growth on three standard laboratory media, including peptone-yeast extract medium (PYE) and minimal salts medium with glucose or xylose as the carbon source. (scu.edu)
CAULOBACTERThe common waterborne bacterium Caulobacter crescentus reproduces asymmetrically. (amazonaws.com)
In the article, "Obstruction of Pilus Retraction Stimulates Bacterial Surface Sensing," a group of interdisciplinary researchers show that in the case of the bacterium Caulobacter crescentus , a model organism that splits its life between swimming and attaching to surfaces, physically blocking the retraction of its pili triggers the attachment to surfaces. (eurekalert.org)
Scientists looked at the bacterium Caulobacter crescentus . (hubpages.com)
In the asymmetrically dividing bacterium Caulobacter crescentus , cell polarity stems from the cell cycle-regulated localization and turnover of signaling protein complexes in these hubs, and yet the mechanisms that establish the identity of the two cell poles have not been established. (asm.org)
The asymmetrically dividing bacterium Caulobacter crescentus uses one such microdomain to link cell cycle progression to morphogenesis, but the mechanism for the generation of this microdomain has remained unclear. (asm.org)
The asymmetrically dividing bacterium Caulobacter crescentus is a model system for single-cell polarity, as every cell division produces two daughter cells that differ in their morphology, replication competency, and size ( 1 ). (asm.org)
Forward genetics and super-resolution microscopy identifies ZitP as a conserved multifunctional regulator that accumulates at both cell extremities in distinct macromolecular structures to perform different functions in the asymmetric model bacterium Caulobacter crescentus . (elifesciences.org)

Genome analysis revealed that the C. crescentus genome encodes a significantly higher number of these signaling proteins (105) than any bacterial genome sequenced thus far. (pnas.org)
The basic paradigm of cell cycle control used by eukaryotic cells, temporally controlled transcription, phosphorylation of regulatory factors, and targeted proteolysis, has been conserved in C. crescentus , although the proteins involved in these processes are different. (pnas.org)
Recent observations of regulatory proteins dynamically localized to defined cellular addresses at specific times in the C. crescentus cell cycle suggest that the three-dimensional organization of this cell adds yet another layer of control ( 5 ). (pnas.org)
From left to right: i) The number of proteins in the reference proteome of Caulobacter crescentus , ii) the total number of models, iii) the number of unique protein sequences for which at least one model is available and iv) a coverage bar plot is shown. (expasy.org)
[1] The S-layer is composed a single protein, RsaA, and is among the most abundant proteins in C. crescentus , accounting for 31% of total cell protein, and up to 51% when multiple copies of rsaA are present. (kenyon.edu)
Due to the non-pathogenic nature of C. crescentus [3] and its high expression of RsaA protein, it has been used to express and assemble foreign proteins at high concentrations. (kenyon.edu)
To test this hypothesis, we generated mutations in the Caulobacter crescentus obg gene ( cgtA C ) which, in Ras-like proteins, would result in either activating or dominant negative phenotypes. (umich.edu)
Although many genes, proteins, and other molecules involved in the asymmetric division exhibited by C. Crescentus have been discovered and characterized during decades, it remains as a challenging task to understand how cell properties arise from the high number of interactions between these molecular components. (ginsim.org)
Cell cycle progression and polar morphogenesis in Caulobacter crescentus are coordinated by the interplay of multiple proteins in time and space. (unibas.ch)
The two proteins DgcB and PleD are the main cyclases in C. crescentus contributing to the intracellular c-di-GMP pool. (unibas.ch)
This work addressed the question, which additional GGDEF domain proteins reveal DGC activity and contribute to the c-di-GMP content in C. crescentus cells. (unibas.ch)
Phylogenetic analysis of the C. crescentus HME-RND proteins showed that CzrA-like proteins, in contrast to those similar to NczA, are almost exclusively found in the Alphaproteobacteria group, and the characteristic protein signatures of each group were highlighted. (beds.ac.uk)
The relative copy numbers of these proteins are essential for complex formation, as overexpression of SpmX in Caulobacter reorganizes the polarity of the cell, generating ectopic cell poles containing PopZ and DivJ. (asm.org)
Prior to cytokinesis in Caulobacter , distinct sets of signaling proteins localize to opposite cell poles where they dictate the cell fate of the nascent daughter cells. (asm.org)

Triplicate cultures of: 1) C. crescentus strain CB15N , and 2) CB15N ∆SpoT, cultured in M2-glucose media, then starved for glucose for 5 minutes. (omicsdi.org)

This report suggests that another outer membrane component, termed the S-layer-associated oligosaccharide (SAO), is the molecule responsible for the attachment of RsaA, the S-layer protein of Caulobacter crescentus , to the cell surface. (springer.com)
The bar plot shows the coverage for every protein in the reference proteome of Caulobacter crescentus for which there is at least one model. (expasy.org)
The S-layer protein of C. crescentus has not yet been visualized by X-Ray crystallography . (kenyon.edu)
We assayed the ability of each RcdA variant to support CtrA proteolysis and polar protein localization in Caulobacter. (nih.gov)
We used high-throughput PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in live Caulobacter crescentus. (epfl.ch)
Here, we identify an atypical two-protein bacteriocin in the α-proteobacterium Caulobacter crescentus that is retained on the surface of producer cells where it mediates cell contact-dependent killing. (elifesciences.org)
These aggregates can drive contact-dependent killing of other organisms, or Caulobacter cells not producing the CdzI immunity protein. (elifesciences.org)
In C. crescentus , a P168V mutant is not activating in vivo , although in vitro , the P168V protein showed a modest reduction in the affinity for GDP. (umich.edu)
Caulobacter crescentus nucleoid: analysis of sedimentation behavior and protein composition during the cell cycle. (microbiologyresearch.org)
The method by which the protein subunits composing the S-layer of C. crescentus, RsaA, interact to form the array and attach to the cell was examined in this thesis. (ubc.ca)
We have solved the X-ray crystal structure of the RNA chaperone protein Hfq from the alpha-proteobacterium Caulobacter crescentus to 2.15 Å resolution, resolving the conserved core of the protein and the entire C-terminal domain (CTD). (cam.ac.uk)
Based on these finding we propose that BipB is a bifunctional protein contributing under the applied conditions with BipC, PleD and DgcB to intracellular c-di-GMP levels in C. crescentus. (unibas.ch)
Here, using genome-wide methods and microscopy of single cells, we show that Caulobacter SMC is recruited to the centromeric parS site and that SMC-mediated arm alignment depends on the chromosome-partitioning protein ParB. (semanticscholar.org)
The Caulobacter crescentus Homolog of DnaA (HdaA) Also Regulates the Proteolysis of the Replication Initiator Protein DnaA. (illumina.com)
Three-dimensional superresolution colocalization of intracellular protein superstructures and the cell surface in live Caulobacter crescentus. (mendeley.com)
By comparison to self-assembling protein networks and polar cell growth mechanisms in other bacterial species, we suggest that the cooligomeric PopZ-SpmX protein complex in Caulobacter illustrates a paradigm for coupling cell cycle progression to the controlled geometry of cell pole establishment. (asm.org)
Further, we find that overexpression of the bridge protein SpmX in Caulobacter disrupts this ordered assembly, generating ectopic cell poles containing both PopZ and DivJ. (asm.org)
In this paper, we investigate the mechanisms governing the localization of PopZ, a chromosome-anchoring protein whose unipolar to bipolar localization pattern is critical for cell cycle progression in Caulobacter crescentus . (rupress.org)

C. crescentus is, to our knowledge, the first free-living α-class proteobacterium to be sequenced and will serve as a foundation for exploring the biology of this group of bacteria, which includes the obligate endosymbiont and human pathogen Rickettsia prowazekii , the plant pathogen Agrobacterium tumefaciens , and the bovine and human pathogen Brucella abortus . (pnas.org)
The genetic separability of the spatial and temporal controls of replication in Caulobacter suggests that DnaA comprises an ancient and phylogenetically widespread control module for replication in almost all bacteria while CtrA developed later in c-proteobacteria and was recruited to enforce replicative asymmetry in daughter cells. (mit.edu)
Partially purified bacterial extracts were prepared from segregated stalked and swarmer bacteria of Caulobacter crescentus and assayed for ATP-dependent deoxyribonuclease activity. (microbiologyresearch.org)
This activity was found to be very low in the swarmer bacteria compared with a pure population of stalked cells or an asynchronous population of C. crescentus. (microbiologyresearch.org)
[2] Since C. crescentus move in places where there is not much to eat, it is believed that these bacteria also use their superglue to pick up nutrients. (listverse.com)
We report the response of C. crescentus to carbon starvation, a common form of nutritional stress encountered by free-living bacteria, that induces stasis. (omicsdi.org)
Instead of dividing two form two identical daughter cells as other bacteria do (a process termed binary division), Caulobacter crescentus undergoes what is termed symmetric division. (encyclopedia.com)
Caulobacter crescentus can be grown in the laboratory so that all the bacteria in the population undergoes division at the same time. (encyclopedia.com)
Like many bacteria, Caulobacter responds to phosphate limitation through a conserved two-component signaling pathway called PhoR-PhoB, but the direct regulon of PhoB in this organism is unknown. (datamed.org)

Polarity of gene transfer in Caulobacter. (microbiologyresearch.org)
Here, we examine if selection acts on G+C contents in Caulobacter crescentus and Pseudomonas aeruginosa , which both have very G+C-rich genomes, by testing whether the expression of gene variants that differ only in their base compositions at synonymous sites affects cellular growth rates. (g3journal.org)
In C. crescentus , expression of the more A+T-rich gene variants decelerated growth, indicating that selection on genic base composition is, in part, responsible for the high G+C content of this genome. (g3journal.org)
Thus, during C. crescentus flagellar biogenesis two different regulatory checkpoints link structural assembly to flagellar gene expression. (beds.ac.uk)
However, our studies show that it likely does not play such a role in Caulobacter as depleting PhoU has no significant effect on PhoB-dependent gene expression. (datamed.org)
The goal of our project is to clone the gene, mreB, from ''Caulobacter crescentus'' (stalked shaped cell) into ''Escherchia coli'' (rod shaped cell) to see if mreB will affect ''E. coli's'' cell shape in some way. (openwetware.org)

Here we describe the functions of three hipBA modules in the alpha-proteobacterium Caulobacter crescentus. (chalmers.se)

The activities of these enzymes were higher in C. crescentus than the fully induced levels observed in Escherichia coli. (yu.edu)
Cloning vectors for studies of Caulobacter crescentus genes should be transferable between Escherichia coli and C. crescentus since a transformation system has not been developed for C. crescentus. (elsevier.com)

The Caulobacter cell cycle regulatory system controls many modular subsystems that organize the progression of cell growth and reproduction. (wikipedia.org)
C. crescentus is a simple and highly manipulable single-celled model system to study cellular differentiation, asymmetric division, and their coordination with cell cycle progression ( 1 , 2 ). (pnas.org)
Periodic activation and deactivation of the essential transcriptional regulator CtrA is necessary to drive cell cycle progression in Caulobacter crescentus. (nih.gov)
A) Morphological progression of the C. crescentus cell cycle. (asm.org)
Although the regulation of the C. crescentus cell cycle has been extensively studied ( 10 ), little is known about how environmental signals impinge upon cell cycle progression. (asm.org)
Mutations in highly conserved regions of FliX could severely affect the recognition between FliX and FlbD and hence interrupt the normal progression of flagellar synthesis and other developmental events in Caulobacter . (beds.ac.uk)
Over fifty genes are required for flagellar biogenesis in C. crescentus , and their temporal and spatial expression is regulated by both cell cycle events and the progression of flagellum assembly. (beds.ac.uk)

Then, a new route for glycolate production was established in E . coli by introducing NAD + -dependent xylose dehydrogenase ( xdh ) and xylonolactonase ( xylC ) from Caulobacter crescentus . (springer.com)
This work demonstrates that a new enzymatic xylose quantification method, based on the activity of xylose dehydrogenase from Caulobacter crescentus , represents an excellent alternative to the manual phloroglucinol reaction. (hindawi.com)

The complete genome sequence of Caulobacter crescentus was determined to be 4,016,942 base pairs in a single circular chromosome encoding 3,767 genes. (pnas.org)
Caulobacter does all that with less than 4,000 genes, allowing full genome-wide studies of a single differentiating cell. (pnas.org)
Circular representation of the C. crescentus genome. (pnas.org)
The genome of C. crescentus was first sequenced in 2001. (expasy.org)
The completion of the entire genome sequences of Chlorobium tepidum and Caulobacter crescentus mark a major milestone in TIGR's history. (thefreedictionary.com)

The taxon is more properly known as Caulobacter vibrioides (Henrici and Johnson 1935). (wikipedia.org)
C. crescentus is synonymous with Caulobacter vibrioides. (wikipedia.org)
C. vibrioides) swarmer and stalked cells starved for carbon GSE25998: Expression data from WT, DSigT and DSigU Caulobacter crescentus (syn. (omicsdi.org)

Caulobacter crescentus is a Gram-negative, oligotrophic bacterium widely distributed in fresh water lakes and streams. (wikipedia.org)
C. crescentus inhabits oligotrophic (i.e., nutrient-poor) environments. (asm.org)
Caulobacter crescentus is an oligotrophic alpha-proteobacterium with a complex cell cycle involving sessile-stalked and piliated, flagellated swarmer cells. (stanford.edu)
These unique biofilm features demonstrate a spatial diversification of the C. crescentus population into a sessile, "stem cell"-like subpopulation (monolayer biofilm), which generates progeny cells capable of exploring the aqueous, oligotrophic environment by swimming motility and a subpopulation accumulating in large mushroom structures. (stanford.edu)

Transcriptional profiling of Caulobacter crescentus during growth on complex and minimal media. (helmholtz-hzi.de)
Transcriptional Profiling of Caulobacter crescentus during Growth on C" by Craig Stephens, Alison K. Hottes et al. (scu.edu)

We report a study by fluorescence imaging and atomic force microscopy on the growth in size and thickness of the holdfast of synchronized Caulobacter crescentus cells as they attach to a glass surface. (biomedcentral.com)

During initial cancer vaccine studies, unexpected results in the control group showed that wild type C. crescentus displayed anti-tumor properties in three different strains of mice, each challenged with a different transplantable tumor model. (kenyon.edu)
Two strains of C. crescentus were shown to utilize fatty acids as sole carbon source and five enzymes involved in a (beta)-oxidation degradative pathway were identified in cell extracts. (yu.edu)
All mutants were successfully expressed in both wild-type and ΔfliX Caulobacter strains. (beds.ac.uk)
Caulobacter strains bearing mutations in fliX are non-motile and do not transcribe class III and IV flagellar genes. (beds.ac.uk)

Caulobacter daughter cells have two very different forms. (wikipedia.org)
Detailed study of the molecular development of these cells as they progress through the cell cycle has enabled researchers to understand Caulobacter cell cycle regulation in great detail. (wikipedia.org)
A new method for recording both fluorescence and cryo-EM images of small bacterial cells was developed and used to identify chemoreceptor arrays in cryotomograms of intact Caulobacter crescentus cells. (nih.gov)
The arrays were always found on the convex side of the cell, further demonstrating that Caulobacter cells maintain dorsal/ventral as well as anterior/posterior asymmetry. (nih.gov)
A) Caulobacter cells divide asymmetrically to yield a swarmer and a stalked cell, which are mixed in culture. (asm.org)
Indeed, a population of C. crescentus cells grown in continuous culture under phosphorus- or nitrogen-limiting conditions accumulates a higher proportion of swarmer cells than is observed in nutrient-replete medium ( 14 , 37 ). (asm.org)
In this work, I use the model organism Caulobacter crescentus to investigate how intracellular asymmetry within the mother cell is translated into the formation of two developmentally distinct daughter cells. (mit.edu)
Caulobacter is an alpha-proteobacterium that always divides asymmetrically to generate two daughter cells that are morphologically distinct and have different replicative capacities. (mit.edu)
Envelope- associated nucleoid from Caulobactercrescentus stalked and swarmer cells. (microbiologyresearch.org)
Caulobacter crescentus is a model organism for the study of asymmetric division and cell type differentiation, as its cell division cycle generates a pair of daughter cells that differ from one another in their morphology and behavior. (ginsim.org)
The stringent response regulator SpoT is required for HipA-mediated antibiotic persistence, but persister cells can form in the absence of all hipBA operons or spoT, indicating that multiple pathways lead to persister cell formation in C. crescentus. (chalmers.se)
In these cells, as in C. crescentus, the individual enzymes form multimers of identical subunits. (yu.edu)
GdhZ co-localizes with FtsZ in late predivisional cells and GdhZ abundance varies during the cell cycle being specifically degraded during DNA replication the period during which the Z-ring is assembled at the division site in C. crescentus. (unamur.be)
Caulobacter crescentus undergoes a series of programmed differentiation events within each cell cycle and generates two dissimilar progeny cells, a motile swarmer cell possessing a single polar flagellum and a sessile stalked cell. (beds.ac.uk)

This study evidences multiple genetic mechanisms of bacterial evolution as driven by selection for growth and survival in a new selective environment and identifies a common polymorphic locus, zwf , between lab-adapted C. crescentus and clinical isolates of Pseudomonas aeruginosa that have adapted to a human host during chronic infection. (asm.org)
As if it were a bacterial version of Spider-Man , C. crescentus has an adhesion force seven times stronger than that of geckos and is three to four times more adhesive than commercial superglue. (listverse.com)
Selection for increased genic G+C-contents in C. crescentus acts independently of the species-specific codon usage pattern and represents an additional selective force operating in bacterial genomes. (g3journal.org)
These projects would not be restricted to bacterial pathogens but would also encompass non-pathogenic bacterial species such as Caulobacter crescentus. (ncl.ac.uk)

We describe the identification of 27 novel Caulobacter crescentus sRNAs by analysis of RNA expression levels assayed using a tiled Caulobacter microarray and a protocol optimized for detection of sRNAs. (semanticscholar.org)
It was originally identified along with 26 other non-coding RNAs using a tiled Caulobacter microarray protocol specifically aimed at detecting small RNAs. (wikipedia.org)

Transfer and expression of Pseudomonas plasmid RP1 in Caulobacter. (microbiologyresearch.org)

In this thesis, I use the model system Caulobacter crescentus to examine the regulation of CtrA, the essential transcription factor at the core of the Caulobacter cell cycle regulatory network. (mit.edu)
The temporal and spatial expression of late flagellar genes in Caulobacter crescentus is activated by the transcription factor FlbD and its partner trans-acting factor FliX. (beds.ac.uk)

The involvement of pili in mushroom architecture is a novel function for type IV pili in C. crescentus. (stanford.edu)
Then, one end of C. crescentus attaches to the surface in question, after which the bacterium is anchored to it through thin structures called pili. (listverse.com)
During their study, the scientists found a new technique to observe and film Caulobacter crescentus pili undergoing dynamic cycles of extension and retraction. (eurekalert.org)

Yet, Caulobacter has the swarmer cell stage that results in slower population growth. (wikipedia.org)
Caulobacter crescentus differentiates from a motile, foraging swarmer cell into a sessile, replication-competent stalked cell during its cell cycle. (asm.org)
Caulobacter crescentus displays a dimorphic life cycle, beginning with a flagellated and chemotactic swarmer cell that neither grows nor replicates its chromosome. (asm.org)

Caulobacter is an important model organism for studying the regulation of the cell cycle, asymmetric cell division, and cellular differentiation. (wikipedia.org)
Modeling Asymmetric Cell Division in Caulobacter crescentus Using a Boolean Logic Approach. (ginsim.org)

C. crescentus invariably differentiates and divides asymmetrically at each cell cycle. (pnas.org)

The link between the City University of New York and Caulobacter crescentus dates back far before this study, however, as Brooklyn College alumnus Lucy Shapiro pioneered the study of Caulobacter crescentus as a model system. (eurekalert.org)

Isolation of mutants of Caulobacter crescentus deficient in ATP-dependent deoxyribonuclease activity. (microbiologyresearch.org)
Our inability to obtain fatty acid degradation mutants, coupled with the high constitutive levels of the (beta)-oxidation enzymes, suggest that fatty acid turnover might play an essential role in membrane biogenesis and cell cycle events in C. crescentus. (yu.edu)
Specific clones from these libraries containing genes of interest were identified by complementation of the appropriate C. crescentus mutants. (elsevier.com)

DipM links peptidoglycan remodelling to outer membrane organization in Caulobacter. (helmholtz-hzi.de)

Biological properties and classification of the Caulobacter group. (microbiologyresearch.org)

The first flagellar assembly checkpoint of Caulobacter crescentus couples assembly of the early class II components of the basal body complex to the expression of class III and IV genes, which encode extracytoplasmic structures of the flagellum. (ingentaconnect.com)
These data indicate that R300B does not contain sequences which would provide promoter function in C. crescentus in the orientation opposite to that of the sul operon and that any genes cloned in this orientation would require native promoters for expression. (elsevier.com)
Glucose availability induced expression of genes encoding enzymes of the Entner-Doudoroff pathway, which was demonstrated here through mutational analysis to be essential in C. crescentus for growth on glucose. (scu.edu)
The PhoB regulon is comprised primarily of genes known or predicted to help Caulobacter scavenge for and import inorganic phosphate, including 15 different membrane transporters. (datamed.org)

C. crescentus was grown on xylose, arabinose and glucose, and maximum specific growth rates determined for the three substrates were 0.11 h −1 , 0.05 h −1 , and 0.15 h −1 respectively. (mdpi.com)
Autolysis of Caulobacter crescentus grown in the presence of glycine. (helmholtz-hzi.de)
This study is measuring the steady-state levels of mRNA in wild-type Caulobacter crescentus grown in M2 defined medium containing either ammonium or nitrate as the sole nitrogen source. (omicsdi.org)
Four independent cultures of Caulobacter crecentus were grown in each of two medium conditions: M2(nitrate)glucose and M2(ammonium)glucose. (omicsdi.org)

Caulobacter crescentus is a gram-negative bacterium that can utilize xylose as a substrate using the Weimberg pathway, which converts xylose to α -ketoglutarate in five steps without carbon loss. (mdpi.com)
Almqvist H, Jonsdottir Glaser S, Tufvegren C, Wasserstrom L, Lidén G. Characterization of the Weimberg Pathway in Caulobacter crescentus . (mdpi.com)
The pathway of fatty acid utilization was investigated in Caulobacter crescentus. (yu.edu)
Thus, fatty acid degradation by the (beta)-oxidation pathway is constitutive in C. crescentus and is only mildly affected by growth in the presence of glucose. (yu.edu)
The enzymes of the (beta)-oxidation pathway in C. crescentus were characterized and three of the enzymes were purified. (yu.edu)
Together these data indicate that PopA adopted a novel role as topology specificity factor to help recruit components of the CtrA degradation pathway to the protease specific old cell pole of C. crescentus. (elsevier.com)

Interestingly, deletion of gdhZ homolog in the facultative intracellular pathogen Brucella abortus (gdhZba) leads to cell division defects, similarly to Caulobacter ∆gdhZ. (unamur.be)

In Caulobacter crescentus , c-di-GMP regulates the synthesis of the polar holdfast adhesin during the cell cycle, yet the molecular and cellular details of this control are currently unknown. (asm.org)
Summary: When Caulobacter crescentus enters S-phase the replication initiation inhibitor CtrA dynamically positions to the old cell pole to be degraded by the polar ClpXP protease. (elsevier.com)
To examine these questions, we focused on the multimeric polar scaffold PopZ, whose dynamic localization pattern plays a crucial role during the cell cycle of Caulobacter crescentus ( Fig. 1 ). (rupress.org)

In fact, aside from an ability to adhere together and form biofilms, no social behavior or cell-cell interaction system such as quorum-sensing has been previously described for Caulobacter . (elifesciences.org)
The text illustrates how the simulation of simple logic models gives valuable insight into the dynamic behavior of the regulatory and signaling networks driving the emergence of the phenotypes exhibited by C. crescentus . (ginsim.org)
Caulobacter crescentus exhibits a distinctive behavior. (encyclopedia.com)

Here we describe a novel c-di-GMP effector, HfsK, that contributes to the cohesive properties and stability of the holdfast adhesin in C. crescentus . (asm.org)

C. crescentus is an interesting organism to study because it inhabits nutrient-poor aquatic environments. (wikipedia.org)
The Caulobacter stalked cell stage provides a fitness advantage by anchoring the cell to surfaces to form biofilms and or to exploit nutrient sources. (wikipedia.org)
Chromosome replication in Caulobacter crescentus growing in a nutrient broth. (microbiologyresearch.org)
Altogether this work illustrates how C. crescentus can adjust cell cycle parameters according to nutrient availability fluctuations. (unamur.be)

These three enzymes form a multienzyme complex in E. coli, but a similar complex was not observed in C. crescentus. (yu.edu)
We also show that C. crescentus Hfq has sRNA binding and RNA annealing activities, and is capable of facilitating the annealing of certain E. coli sRNA:mRNA pairs in vivo. (cam.ac.uk)

[3] In the Lewis lung carcinoma model, 60% of the mice pre-immunized with live C. crescentus were still alive by day 90, whereas all mice pre-immunized with heat killed C. crescentus or the saline control died by 8 weeks post tumor challenge. (kenyon.edu)
[3] In the murine L1210 leukemia model, the entire control group only survived until the fourth week, whereas approximately 80% of mice pre-immunized with live C. crescentus survived, and 100% of the heat-killed immunized mice survived the entire 3-month study period. (kenyon.edu)
flgH), a model for biofilm formation in C. crescentus is proposed. (stanford.edu)

Caulobacter crescentus is a Gram-negative rod-like bacterium that inhabits fresh water. (encyclopedia.com)

Analysis of the dynamics of TrfA- complex formation during the Caulobacter cell cycle revealed that TrfA binds primarily during the G1 phase, however plasmid DNA synthesis occurs during the S and G2 phases of the cell cycle. (unibas.ch)

To quantitatively explore the correlation between cell kinematics and motility, we use a 3D tracking microscope-a digital implementation of the instrument first developed by Berg ( 11 )-to follow individuals of Caulobacter crescentus for extended periods of time. (pnas.org)

Small non-coding RNAs in Caulobacter crescentus. (semanticscholar.org)
CrfA RNA (Caulobacter response to famine RNA) is a family of non-coding RNAs found in Caulobacter crescentus. (wikipedia.org)

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Chromosome methylation and measurement of faithful, once and only once per cell cycle chromosome replication in Caulobacter crescentus. (1/444)

The CtrA response regulator mediates temporal control of gene expression during the Caulobacter cell cycle. (2/444)

Cell cycle-dependent polar localization of an essential bacterial histidine kinase that controls DNA replication and cell division. (3/444)

Identification and transcriptional control of the genes encoding the Caulobacter crescentus ClpXP protease. (4/444)

Cell cycle expression and transcriptional regulation of DNA topoisomerase IV genes in caulobacter. (5/444)

Feedback control of a master bacterial cell-cycle regulator. (6/444)

Regulation of podJ expression during the Caulobacter crescentus cell cycle. (7/444)

Bacterial cells: The migrating kinase and the master regulator. (8/444)

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Caulobacter crescentus

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Flagella

Bacterial Proteins

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Gene Expression Regulation, Bacterial

Flagellin

Genes, Bacterial

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Chromosomes, Bacterial

Alphaproteobacteria

Molecular Sequence Data

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Site-Specific DNA-Methyltransferase (Adenine-Specific)

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Genetic Complementation Test

Potassium Acetate

Promoter Regions, Genetic

Cell Division

Phosphotungstic Acid

Vanillic Acid

Replication Origin

DNA Transposable Elements

Sigma Factor

Galactose Dehydrogenases

Phosphorus-Oxygen Lyases

Sequence Homology, Amino Acid

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Logic

Chromosome methylation and measurement of faithful, once and only once per cell cycle chromosome replication in Caulobacter crescentus. (1/444)

The CtrA response regulator mediates temporal control of gene expression during the Caulobacter cell cycle. (2/444)

Cell cycle-dependent polar localization of an essential bacterial histidine kinase that controls DNA replication and cell division. (3/444)

Identification and transcriptional control of the genes encoding the Caulobacter crescentus ClpXP protease. (4/444)

Cell cycle expression and transcriptional regulation of DNA topoisomerase IV genes in caulobacter. (5/444)

Feedback control of a master bacterial cell-cycle regulator. (6/444)

Regulation of podJ expression during the Caulobacter crescentus cell cycle. (7/444)

Bacterial cells: The migrating kinase and the master regulator. (8/444)

Dynamics and control of biofilms of the oligotrophic bacterium Caulobacter crescentus. | Alfred M. Spormann Laboratory

Use of transmissible plasmids as cloning vectors in Caulobacter crescentus<...

High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization<...

ASMscience | Regulation of the Caulob

hipBA toxin-antitoxin systems mediate persistence in Caulobacter crescentus

Flagellin redundancy in Caulobacter crescentus and its implications for flagellar filament assembly<...

Role of Transcription in the Temporal Control of Development in Caulobacter crescentus | PNAS

Mutations that alter RcdA surface residues decouple protein localization and CtrA proteolysis in Caulobacter crescentus

SMC Progressively Aligns Chromosomal Arms in Caulobacter crescentus but Is Antagonized by Convergent Transcription - Semantic...

Glucoamylase of Caulobacter crescentus CB15: cloning and expression in Escherichia coli and functional identification | AMB...

Deaths in the family cause bacteria to flee: IU News Room: Indiana University

Factors controlling in vitro recrystallization of the Caulobacter crescentus paracrystalline S-layer. | Journal of Bacteriology

Complete genome sequence of Caulobacter crescentus | PNAS

Math model accurately mimics cell division in carbon-cycling bacterium | EurekAlert! Science News

Urs Jenal - Wikipedia

Publications - Jonas Lab

Cell Cycle Control of a Holdfast Attachment Gene inCaulobacter crescentus | Journal of Bacteriology

Caulobacter crescentus - Wikipedia

Distinct constrictive processes, separated in time and space, divide caulobacter inner and outer membranes | Meta

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