A species of gram-negative, aerobic bacteria that consist of slender vibroid cells.
A genus of gram-negative, aerobic, rod- or vibroid-shaped or fusiform bacteria that commonly produce a stalk. They are found in fresh water and soil and divide by binary transverse fission.
A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
Proteins found in any species of bacterium.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
A protein with a molecular weight of 40,000 isolated from bacterial flagella. At appropriate pH and salt concentration, three flagellin monomers can spontaneously reaggregate to form structures which appear identical to intact flagella.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
The functional hereditary units of BACTERIA.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
A class in the phylum PROTEOBACTERIA comprised mostly of two major phenotypes: purple non-sulfur bacteria and aerobic bacteriochlorophyll-containing bacteria.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Physiological processes and properties of BACTERIA.
An enzyme responsible for producing a species-characteristic methylation pattern on adenine residues in a specific short base sequence in the host cell DNA. The enzyme catalyzes the methylation of DNA adenine in the presence of S-adenosyl-L-methionine to form DNA containing 6-methylaminopurine and S-adenosyl-L-homocysteine. EC 2.1.1.72.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A family of stalked bacteria that reproduces by budding. There are four genera: CAULOBACTER, Asticcacaulis, Brevundimonas, and Phenylobacterium.
The process by which a DNA molecule is duplicated.
A large group of aerobic bacteria which show up as pink (negative) when treated by the gram-staining method. This is because the cell walls of gram-negative bacteria are low in peptidoglycan and thus have low affinity for violet stain and high affinity for the pink dye safranine.
A DNA-directed RNA polymerase found in BACTERIA. It is a holoenzyme that consists of multiple subunits including sigma factor 54.
Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.
An ATP-dependent protease found in prokaryotes, CHLOROPLASTS, and MITOCHONDRIA. It is a soluble multisubunit complex that plays a role in the degradation of many abnormal proteins.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Viruses whose hosts are bacterial cells.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
A unique DNA sequence of a replicon at which DNA REPLICATION is initiated and proceeds bidirectionally or unidirectionally. It contains the sites where the first separation of the complementary strands occurs, a primer RNA is synthesized, and the switch from primer RNA to DNA synthesis takes place. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Water containing no significant amounts of salts, such as water from RIVERS and LAKES.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
A family of bacteriophages that infects enterobacteria, CAULOBACTER, and PSEUDOMONAS. The genome consists of linear, positive-sense single-stranded RNA.
A family of gram-negative bacteria usually found in soil or water and including many plant pathogens and a few animal pathogens.
A flavoring agent. It is the intermediate product in the two-step bioconversion of ferulic acid to vanillin. (J Biotechnol 1996;50(2-3):107-13).
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
Pollutants, present in water or bodies of water, which exhibit radioactivity.
Enzymes that catalyze the cleavage of a phosphorus-oxygen bond by means other than hydrolysis or oxidation. EC 4.6.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
A protein which is a subunit of RNA polymerase. It effects initiation of specific RNA chains from DNA.
A potassium salt used to replenish ELECTROLYTES, for restoration of WATER-ELECTROLYTE BALANCE, as well as a urinary and systemic alkalizer, which can be administered orally or by intravenous infusion. Formerly, it was used in DIURETICS and EXPECTORANTS.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
The salinated water of OCEANS AND SEAS that provides habitat for marine organisms.
Tungsten hydroxide oxide phosphate. A white or slightly yellowish-green, slightly efflorescent crystal or crystalline powder. It is used as a reagent for alkaloids and many other nitrogen bases, for phenols, albumin, peptone, amino acids, uric acid, urea, blood, and carbohydrates. (From Merck Index, 11th ed)
The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.
Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.
Bacterial proteins that are used by BACTERIOPHAGES to incorporate their DNA into the DNA of the "host" bacteria. They are DNA-binding proteins that function in genetic recombination as well as in transcriptional and translational regulation.

The phylogenetic relationships of Caulobacter, Asticcacaulis and Brevundimonas species and their taxonomic implications. (1/72)

The phylogenetic relationships among the species of Caulobacter, Asticcacaulis and Brevundimonas were studied by comparison of their 16S rDNA sequences. The analysis of almost complete sequences confirmed the early evolutionary divergence of the freshwater and marine species of Caulobacter reported previously [Stahl, D. A., Key, R., Flesher, B. & Smit, J. (1992). J Bacteriol 174, 2193-2198]. The freshwater species formed two distinct clusters. One cluster contained the species Caulobacter bacteroides, Caulobacter crescentus, Caulobacter fusiformis and Caulobacter henricii. C. bacteroides and C. fusiformis are very closely related (sequence identity 99.8%). The second cluster was not exclusive and contained the specis Caulobacter intermedius, Caulobacter subvibrioides and Caulobacter variabilis, as well as Brevundimonas diminuta and Brevundimonas vesicularis. The marine species Caulobacter halobacteroides and Caulobacter maris were very closely related, with a sequence identity of 99.7%. These two species were most closely but distantly related to the marine hyphal/budding bacteria Hyphomonas jannaschiana and Hirschia baltica, which formed a deep phylogenetic line with Rhodobacter sphaeroides and Rhodobacter capsulatus. Caulobacter leidyia is unrelated to the other species of Caulobacter and belongs to the alpha-4 subclass of the Proteobacteria, forming a distinct cluster with Asticcacaulis excentricus and Asticcacaulis biprosthecium. The taxonomic implications of the polyphyletic nature of the genus Caulobacter and the absence of a type culture for the type species of the genus Caulobacter vibrioides, are discussed.  (+info)

Phylogeny and polyphasic taxonomy of Caulobacter species. Proposal of Maricaulis gen. nov. with Maricaulis maris (Poindexter) comb. nov. as the type species, and emended description of the genera Brevundimonas and Caulobacter. (2/72)

The genus Caulobacter is composed of prosthecate bacteria often specialized for oligotrophic environments. The taxonomy of Caulobacter has relied primarily upon morphological criteria: a strain that visually appeared to be a member of the Caulobacter has generally been called one without challenge. A polyphasic approach, comprising 16S rDNA sequencing, profiling restriction fragments of 16S-23S rDNA interspacer regions, lipid analysis, immunological profiling and salt tolerance characterizations, was used to clarify the taxonomy of 76 strains of the genera Caulobacter. Brevundimonas, Hyphomonas and Mycoplana. The described species of the genus Caulobacter formed a paraphyletic group with Caulobacter henricii, Caulobacter fusiformis, Caulobacter vibrioides and Mycoplana segnis (Caulobacter segnis comb. nov.) belonging to Caulobacter sensu stricto. Caulobacter bacteroides (Brevundimonas bacteroides comb. nov.), C. henricii subsp. aurantiacus (Brevundimonas aurantiaca comb. nov.), Caulobacter intermedius (Brevundimonas intermedia comb. nov.), Caulobacter subvibrioides (Brevundimonas subvibrioides comb. nov.), C. subvibrioides subsp. albus (Brevundimonas alba comb. nov.), Caulobacter variabilis (Brevundimonas variabilis comb. nov.) and Mycoplana bullata belong to the genus Brevundimonas. The halophilic species Caulobacter maris and Caulobacter halobacteroides are different from these two genera and form the genus Maricaulis gen. nov. with Maricaulis maris as the type species. Caulobacter leidyia was observed to cluster with species of the genus Sphingomonas. Caulobacter crescentus is synonymous with C. vibrioides and C. halobacteroides is synonymous with Maricaulis maris as determined by these analyses and DNA-DNA hybridization. Biomarkers discerning these different genera were determined. The necessary recombinations have been proposed and a description of Maricaulis is presented.  (+info)

Regulation of stalk elongation by phosphate in Caulobacter crescentus. (3/72)

In Caulobacter crescentus, stalk biosynthesis is regulated by cell cycle cues and by extracellular phosphate concentration. Phosphate-starved cells undergo dramatic stalk elongation to produce stalks as much as 30 times as long as those of cells growing in phosphate-rich medium. To identify genes involved in the control of stalk elongation, transposon mutants were isolated that exhibited a long-stalk phenotype irrespective of extracellular phosphate concentration. The disrupted genes were identified as homologues of the high-affinity phosphate transport genes pstSCAB of Escherichia coli. In E. coli, pst mutants have a constitutively expressed phosphate (Pho) regulon. To determine if stalk elongation is regulated by the Pho regulon, the Caulobacter phoB gene that encodes the transcriptional activator of the Pho regulon was cloned and mutated. While phoB was not required for stalk synthesis or for the cell cycle timing of stalk synthesis initiation, it was required for stalk elongation in response to phosphate starvation. Both pstS and phoB mutants were deficient in phosphate transport. When a phoB mutant was grown with limiting phosphate concentrations, stalks only increased in length by an average of 1.4-fold compared to the average 9-fold increase in stalk length of wild-type cells grown in the same medium. Thus, the phenotypes of phoB and pst mutants were opposite. phoB mutants were unable to elongate stalks during phosphate starvation, whereas pst mutants made long stalks in both high- and low-phosphate media. Analysis of double pst phoB mutants indicated that the long-stalk phenotype of pst mutants was dependent on phoB. In addition, analysis of a pstS-lacZ transcriptional fusion showed that pstS transcription is dependent on phoB. These results suggest that the signal transduction pathway that stimulates stalk elongation in response to phosphate starvation is mediated by the Pst proteins and the response regulator PhoB.  (+info)

Microbial community changes in biological phosphate-removal systems on altering sludge phosphorus content. (4/72)

Biomarkers (respiratory quinones and cellular fatty acids) and denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA genes were used to characterize the microbial community structure of lab-scale enhanced biological phosphate-removal (EBPR) systems in response to altering sludge phosphorus (P) content. All the data suggest that the microbial community structures of sludge samples with a P content between 8 and 12.3% (sludge dry weight) (i.e. good EBPR activity) were very similar, but differed from those with 2% P content (i.e. no EBPR activity). For all samples analysed, ubiquinones Q-8 and Q-10, menaquinone MK-8(H4), and fatty acids C16:0, C16:1 omega9c and C18:1, omega11c were the major components. The dominance of Q-8, Q-10 and MK-8(H4) suggested that large numbers of organisms belonging to the beta and alpha subclasses of the Proteobacteria and the Actinobacteria from the high G+C Gram-positive bacteria, respectively, were present. DGGE analysis revealed at least 7-9 predominant DNA bands and numerous other fragments in each sample. Five major DGGE fragments from each of the 2% and 12% P-containing sludge samples, respectively, were successfully isolated and sequenced. Phylogenetic analysis of the sequences indicated that both 2% and 12% P-containing sludge samples shared three common phylotypes that were separately affiliated with a novel bacterial group from the gamma subclass of the Proteobacteria, two MK-8(H4)-containing actinobacteria previously isolated from the 2% P-containing sludge, and a Caulobacter spp. in the alpha subclass of the Proteobacteria. The phylogenetic analysis also revealed phylotypes unique to both sludge samples. Changes in sludge P content therefore had an effect on the composition and abundance of the predominant microbial populations, though specific phylotypes could not be unequivocally associated with EBPR.  (+info)

In situ reproductive rate of freshwater Caulobacter spp. (5/72)

Electron microscope grids were submerged in Lake Washington, Seattle, Wash., in June 1996 as bait to which Caulobacter sp. swarmers would attach and on which they would then reproduce in situ. Enumeration of bands in the stalks of attached cells implied that the caulobacters were completing approximately three reproductive cycles per day. A succession of morphological types of caulobacters occurred, as well as an episode of bacteriovore grazing that slowed the accumulation of caulobacters and prevented the aging of the population.  (+info)

Determination of the systematic position of the genus Asticcacaulis Poindexter by a polyphasic analysis. (6/72)

The genus Asticcacaulis, to date, comprises two species of unicellular, stalked bacteria, developing a stalk at a site which is not coincidental with the centre of the pole of the cell. Multiplication is similar to that demonstrated by the prosthecate species of the genera Caulobacter, Brevundimonas and Maricaulis. A polyphasic approach, comprising 16S rRNA gene sequencing, lipid analysis and NaCl tolerance characterizations, was used to clarify the taxonomy of the two Asticcacaulis species. From the analysis of the 16S rRNA gene sequences, a close phylogenetic relationship between the species that comprise the genera Asticcacaulis, Caulobacter and Brevundimonas could be deduced wherein the three genera form three distinct branches. The individual genera could also be discerned by different characteristic polar lipids. The species of Asticcacaulis differed from species of Caulobacter and Brevundimonas by the lack of 1,2-diacyl-3-O-[6'-phosphatidyl-alpha-D-glucopyranosyl]glycerol. They also did not contain 1,2-di-O-acyl-3-O-[D-glucopyranosyl-(1-->4)-alpha-D-glucuronopyranosyl]glycerol, which is found in most Brevundimonas species but not in strains of the genus Caulobacter. The morphological differences seen between the two species Asticcacaulis excentricus and Asticcacaulis biprosthecium are mirrored by the observed 16S rDNA sequence similarity value of 95.3%, which is relatively low compared to the interspecies similarity values observed within the genera Brevundimonas or Caulobacter.  (+info)

New members of the ctrA regulon: the major chemotaxis operon in Caulobacter is CtrA dependent. (7/72)

The Caulobacter crescentus che promoter region consists of two divergent promoters, directing expression of the major chemotaxis operon and a novel gene cagA (chemotaxis associated gene A). Analyses of start sites by primer extension and alignment of the divergent promoters revealed significant similarities between them at the -35 promoter region. Both mcpA and cagA are differentially expressed in the cell cycle, with maximal activation of transcription in predivisional cells. The main difference between the mcpA and cagA promoters is that, in common with the fljK flagellin, cagA is expressed in swarmer cells. A cagA--lacZ promoter fusion that contains 36 bases of untranslated mRNA has sufficient information to segregate the lacZ transcript to swarmer cells. Expression of mcpA and cagA was dependent on DNA replication. Transcriptional epistasis experiments were performed to identify potential regulators in the flagellar hierarchy. The sigma factor RpoN, which is required for flagellar biogenesis, is not required for mcpA and cagA expression. Mutations in the genes for the MS-ring and the switch complex (flagellar class II mutants) do not affect expression of mcpA and cagA. However, CtrA, an essential response regulator of flagellar gene transcription, is required.  (+info)

Genes directly controlled by CtrA, a master regulator of the Caulobacter cell cycle. (8/72)

Studies of the genetic network that controls the Caulobacter cell cycle have identified a response regulator, CtrA, that controls, directly or indirectly, one-quarter of the 553 cell cycle-regulated genes. We have performed in vivo genomic binding site analysis of the CtrA protein to identify which of these genes have regulatory regions bound directly by CtrA. By combining these data with previous global analysis of cell cycle transcription patterns and gene expression profiles of mutant ctrA strains, we have determined that CtrA directly regulates at least 95 genes. The total group of CtrA-regulated genes includes those involved in polar morphogenesis, DNA replication initiation, DNA methylation, cell division, and cell wall metabolism. Also among the genes in this notably large regulon are 14 that encode regulatory proteins, including 10 two-component signal transduction regulatory proteins. Identification of additional regulatory genes activated by CtrA will serve to directly connect new regulatory modules to the network controlling cell cycle progression.  (+info)

Prosthecobacter fusiformis is morphologically similar to caulobacters; however, it lacks a dimorphic life cycle. To determine the relatedness of the genus Prosthecobacter to dimorphic caulobacters and other prosthecate members of the alpha subgroup of the Proteobacteria (alpha-Proteobacteria), we is …
ID CAUSK_1_PE109 STANDARD; PRT; 537 AA. AC CAUSK_1_PE109; B0T9X1; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Drug resistance transporter, EmrB/QacA subfamily;Flags: DE Precursor; (CAUSK_1.PE109). GN OrderedLocusNames=Caul_5403; OS CAULOBACTER SP. K31. OC Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; OC Caulobacteraceae; Caulobacter. OX NCBI_TaxID=366602; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS CAUSK_1.PE109. CC Caulobacter sp. K31 plasmid pCAUL02, complete sequence. CC (1809 nt) ; CC -!- ANNOTATIONS ORIGIN:B0T9X1_CAUSK CC -!- GENE_FAMILY: HOG000219979 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; B0T9X1; -. DR EMBL; CP000929; ABZ74520.1; -; Genomic_DNA. DR RefSeq; YP_001672179.1; NC_010333.1. DR STRING; B0T9X1; -. DR GeneID; 5897187; -. DR GenomeReviews; CP000929_GR; Caul_5403. DR KEGG; cak:Caul_5403; -. DR OMA; HAGNSAT; -. DR ...
C R Maliszewski, G J Delespesse, M A Schoenborn, R J Armitage, W C Fanslow, T Nakajima, E Baker, G R Sutherland, K Poindexter and C Birks ...
One is reminded of Buster Poindexter today: hot hot hot. The day game today should be miserably hot with the temperatures expected to reach well over a hundred. Feels like its 145 already. And Hanley Ramirez; since coming back from the DL, hes hot hot hot, including dropping a 3-run bomb last night estimated at 441 feet and seeming to get a hit every time hes up ...
One is reminded of Buster Poindexter today: hot hot hot. The day game today should be miserably hot with the temperatures expected to reach well over a hundred. Feels like its 145 already. And Hanley Ramirez; since coming back from the DL, hes hot hot hot, including dropping a 3-run bomb last night estimated at 441 feet and seeming to get a hit every time hes up ...
The Caulobacter cell cycle regulatory system controls many modular subsystems that organize the progression of cell growth and reproduction. A control system constructed using biochemical and genetic logic circuitry organizes the timing of initiation of each of these subsystems. The central feature of the cell cycle regulation is a cyclical genetic circuit-a cell cycle engine-that is centered around the successive interactions of five master regulatory proteins: DnaA, GcrA, CtrA, SciP, and CcrM whose roles were worked out by the laboratories of Lucy Shapiro and Harley McAdams.[8][9][10] These five proteins directly control the timing of expression of over 200 genes. The five master regulatory proteins are synthesized and then eliminated from the cell one after the other over the course of the cell cycle. Several additional cell signaling pathways are also essential to the proper functioning of this cell cycle engine. The principal role of these signaling pathways is to ensure reliable production ...
The requirement for transcription during development of the stalked bacterium, Caulobacter crescentus, was studied with synchronous cultures of swarmer cells. The developmental pattern in these bacteria was first established by determination of the times at which specific changes in cell structure and function occurred. These changes could be divided into those characteristic of (a) development of the swarmer cell into the stalked cell: loss of motility and synthesis of the stalk, and (b) development of the stalked cell into the asymmetric dividing cell: chromosome replication, synthesis of the flagellum, motility, and division. The effect of rifampin in blocking several of these steps-loss of motility, initiation of chromosome replication, and cell division-indicates that RNA synthesis is required throughout the cell cycle for normal differentiation.. ...
The S-layer of Caulobacter is a two-dimensional paracrystalline array on the cell surface composed of a single protein, RsaA. We have established conditions for preparation of stable, soluble protein and then efficient in vitro recrystallization of the purified protein. Efficient recrystallization and long range order could not be obtained with pure protein only, though it was apparent that calcium was required for crystallization. Recrystallization was obtained when lipid vesicles were provided, but only when the vesicles contained the specific species of Caulobacter smooth lipopolysaccharide (SLPS) that previous studies implicated as a requirement for attaching the S-layer to the cell surface. The specific type of phospholipids did not appear critical; phospholipids rather different from those present in Caulobacter membranes or archaebacterial tetraether lipids worked equally well. The source of LPS was critical; rough and smooth variants of Salmonella typhimurium LPS as well as the rough ...
TY - JOUR. T1 - Use of transmissible plasmids as cloning vectors in Caulobacter crescentus. AU - Schoenlein, Patricia V. AU - Gallman, Lilly M.. AU - Ely, Bert. PY - 1988/10/30. Y1 - 1988/10/30. N2 - Cloning vectors for studies of Caulobacter crescentus genes should be transferable between Escherichia coli and C. crescentus since a transformation system has not been developed for C. crescentus. We have tested a large number of vectors containing IncP or IncQ replicons and found that many of the vectors containing IncQ replicons, and all but one of the vectors containing IncP replicons, are readily transferred by conjugation into C. crescentus. All of the plasmids tested were maintained in C. crescentus at 1 to 5 copies per cell, but plasmids containing IncP replicons were more stable than plasmids containing IncQ replicons. Further studies with a derivative of the IncQ plasmid R300B showed that when a promoterless kanamycin (Km)-resistance gene (npt2) was inserted into the intercistronic region ...
The structural maintenance of chromosomes (SMC) complex plays an important role in chromosome organization and segregation in most living organisms. In Caulobacter crescentus, SMC is required to align the left and the right arms of the chromosome that run in parallel down the long axis of the cell. However, the mechanism of SMC-mediated alignment of chromosomal arms remains elusive. Here, using genome-wide methods and microscopy of single cells, we show that Caulobacter SMC is recruited to the centromeric parS site and that SMC-mediated arm alignment depends on the chromosome-partitioning protein ParB. We provide evidence that SMC likely tethers the parS-proximal regions of the chromosomal arms together, promoting arm alignment. Furthermore, we show that highly transcribed genes near parS that are oriented against SMC translocation disrupt arm alignment, suggesting that head-on transcription interferes with SMC translocation. Our results demonstrate a tight interdependence of bacterial chromosome
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Periodic activation and deactivation of the essential transcriptional regulator CtrA is necessary to drive cell cycle progression in Caulobacter crescentus. At the onset of DNA replication (the G1-S cell cycle transition), CtrA and the AAA+ protease ClpXP colocalize at one cell pole along with three …
COGEM released a comprehensive database of pathogenicity assessment of around 2575 bacterial species in 2011. The database ranks the pathogenicity of species on a scale of 1 to 4 - 1 being not belonging to a recognized group of disease-invoking agents in humans or animals and having an extended history of safe usage and 4 being a species that can cause a very serious human disease, for which no prophylaxis is known ...
Author: Luo, L. et al.; Genre: Journal Article; Published in Print: 2004-06; Keywords: Geminin; Hox; Polycomb; Cdt1; cell cycle; embryonic patterning; coordination|br/|; Title: Geminin coordinates cell cycle and developmental control
The biochemical properties of the maltodextrin-hydrolyzing enzymes of cold-tolerant proteobacterium Caulobacter crescentus CB15 remain to be elucidated, although whose maltodextrin transport systems were well investigated. We cloned the putative glucoamylase of C. crescentus CB15 (CauloGA) gene. The CauloGA gene product that was expressed in E. coli was prone to forming inclusion bodies; however, most of the gene product was expressed in a soluble and active form when it was expressed as a fusion protein with Staphylococcus Protein A. The fusion protein was purified using an IgG Sepharose column and was identified as the active GA. The optimum temperature and pH for the activity of this GA toward maltotriose as a substrate were approximately 40°C and 5.0, respectively, and a differential scanning fluorimetry (DSF) analysis revealed that the melting temperature (Tm) of CauloGA was 42.9°C. The kinetic analyses with maltotriose and other maltodextrins as the substrates indicated that CauloGA has higher
https://doi.org/10.1101/436394. Felletti M., Omnus D.J. and Jonas K.§ (2019). Regulation of the replication initiator DnaA in Caulobacter crescentus. BBA Gene Regulatory Mechanisms. 1862(7):697-705. [link]. Schramm F.D., Heinrich K., Thüring M., Bernhardt J. and Jonas K.§ (2017). An essential regulatory function of the DnaK chaperone dictates the decision between proliferation and maintenance in Caulobacter crescentus. PLOS Genetics. 13:e1007148. [link]. Heinrich K., Sobetzko P. and Jonas K.§ (2016). A Kinase-Phosphatase Switch Transduces Environmental Information into a Bacterial Cell Cycle Circuit. PLOS Genetics. 12: e1006522. [link]. Liu J., Francis L.I., Jonas K., Laub M.T. and Chien P. (2016). ClpAP is an auxiliary protease for DnaA degradation in Caulobacter crescentus. Molecular Microbiology. 102:1075-1085 [link]. Heinrich K. and Jonas K.§ (2016) Bakterielle Überlebensstrategien - Zellzykluskontrolle als Stressantwort. BIOspektrum. 22: 41-43 [link]. Leslie D.J.*, Heinen C.*, Schramm ...
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Dr. Anthony E Poindexter, MD, rated 3.9/5 by patients. 12 reviews, Phone number & practice locations, Nephrologist in Springfield, MA.
This chapter focuses on the aquatic bacterium Caulobacter crescentus, which divides asymmetrically during each cell division cycle, yielding progeny cells that differ both structurally and functionally. The initially motile swarmer cell progeny sheds its flagellum and differentiates into a nonmotile stalked cell. In addition to morphological differences, the stalked- and swarmer cell progeny inherit different competencies for chromosome replication. A central component of any cell cycle is the initiation of chromosome replication coupled with strict controls to prevent repeated rounds of DNA replication without intervening cell divisions. The Caulobacter origin of replication was identified and cloned by taking advantage of the observation that replication is always initiated in the stalked cell. Microbial cells are able to monitor changes in their environment, detect changes in cell density, and communicate with each other and with other organisms through signals. The Caulobacter DnaA protein is a
Another factor that likely shapes the composition of the plant microbiome is interaction between microbes. In 2016, Eric Kemen of the Max Planck Institute for Plant Breeding Research and colleagues surveyed the microbes thriving in and on wild Arabidopsis leaves at five natural sites in Germany sampled in different seasons. They then plotted correlations between the abundances of more than 90,000 pairs of microbial genera identified in their survey, revealing six microbial hubs-nodes with significantly more connections than other nodes within the network. These hubs were represented by the oomycete genus Albugo, the fungal genera Udeniomyces and Dioszegia, the bacterial genus Caulobacter, and two distinct members of the bacterial order Burkholderiales.5 Given the high degree of connectivity within the communities, it is likely that these microbial hubs play a disproportionate role in the microbiome, akin to that of keystone species in an ecosystem ...
The holdfast is important for the attachment of C. crescentus cells to surfaces; however, little is known about the regulation of holdfast synthesis and holdfast attachment genes during the cell cycle. Furthermore, the time of holdfast appearance at the pole of the cell is not known. In this paper, we investigate two aspects of holdfast synthesis: the transcription of a holdfast attachment gene, hfaA, and the appearance of the holdfast at the pole of the cell. We demonstrate that hfaA transcription is temporally controlled during the cell cycle, with maximal transcription in the swarmer pole of the predivisional cell. We show that the holdfast is not present in swarmer cells or at the swarmer poles of predivisional cells. Our results suggest that the holdfast appears during the differentiation of swarmer to stalked cells.. The hfaA gene possesses a ς54 promoter sequence at the requisite distance from the transcription start site (16). However, our results indicate that hfaA is not transcribed ...
Caulobacter is a single-celled organism with a filament-like tail called a flagellum. As it swims, its rounded cellular head rotates in one direction, while the tail rotates in the opposite direction. This creates torque, which helps explain the bacteriums nonlinear movement through a fluid. What Tang and his team found, however, is that Caulobacter also is influenced by Brownian motion, which is the zigzagging motion that occurs when immersed particles are buffeted by the actions of the molecules of the surrounding medium. What that means, in effect, is that Caulobacter is being pinballed by the water molecules surrounding it as it swims ...
The SWISS-MODEL Repository is a database of annotated 3D protein structure models generated by the SWISS-MODEL homology-modelling pipeline
Wilkes, D., Crabb, D., Rex, D., Einhorn, L., Zipes, D., Benson, M., Moe, S., Abonour, R., Farlow, M., Miller, K., Loehrer, P., Cripe, L., Peacock, M., DiMeglio, L., Econs, M., Spinola, S., de Cordon, K., Denne, S., Kwo, P., Agarwal, R., Considine, R., Poindexter, B., Stevens, J., Kraus, M., Chalasani, N., Robertson, M., Logan, T., Croop, J., Groh, W., Robertson, M., Hannon, T., Wojcieszek, J., Considine, R., Mather, K., Chalasani, N., Flockhart, D. A., Foroud, T., Desta, Z., Benson, M., Kareken, D., Poindexter, B., Friedman, A., Gupta, S., Schneider, B., Carpenter, J., Steiner, S., Storniolo, A. M., Cripe, L., Murphy, M., Molleston, J., Hui, S., Von Ah, D., Logan, T., Sawada, S., Fineberg, S. E., Edmondson, J. W., Lumeng, L., OConnor, K., Clark, C. M., Maxwell, D., Scott, P., Prystowsky, E., Hoffman, R., Brater, C. D., Brandt, I., Bradley, J., Vinicor, F., Williams, S., Weir, C., Heger, J., Thomasson, H., Manolagas, S., Duckworth, W., MacLaren, N., Mohler, E., Baker, J., Vargo, D., Lehman, G., ...
President Reagan asked the Senate Intelligence Committee on Tuesday to grant limited immunity to two former National Security Council aides so they will testify on the secret Iran arms deal and
Principal Investigator:NAGAI Kazuo, Project Period (FY):1993 - 1995, Research Category:Grant-in-Aid for General Scientific Research (B), Research Field:応用微生物学・応用生物化学
Kühn, Juliane and Briegel, Ariane and Mörschel, Erhard et al. (2010) Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus. EMBO Journal, 29 (2). pp. 327-339. ISSN 0261-4189. PMCID PMC2824468. https://resolver.caltech.edu/CaltechAUTHORS:20100218-083022805 ...
1MB0: Crystallographic and Biochemical Studies of DivK Reveal Novel Features of an Essential Response Regulator in Caulobacter crescentus.
Homogenized halides and alkali cation segregation in alloyed organic- inorganic perovskites, J.-P. Correa-Baena†*, Y. Luo†, T. M. Brenner, J. Snaider, S. Sun, X. Li, M. A. Jensen, N. T. P. Hartono, L. Nienhaus, S. Wieghold, J. R. Poindexter, S. Wang, Y. S. Meng, T. Wang, B. Lai, M. V. Holt, Z. Cai, M. G. Bawendi, L. Huang, T. Buonassisi*, D. P. Fenning*, Science, 363, 6427, pp. 627-631, 2019 ...
Buster Poindexter becomes David Johansen once again, changing his name back and donning a tiny T-shirt and tight jeans. Hes even grown his hair out--all so he can try to squeeze a bit more cash out of the New York Dolls legacy with a reunion album. Sylvain Sylvain, the only other Doll of old still among the living, joins Johansen here, as does Hanoi Rocks bassist Sammi Yaffa. One Day It Will Please Us to Remember Even This sounds like the worst of Johansens solo efforts--that is to say great, simple rock n roll backing awkward, self-indulgent vocals that grate on the nerves ever more with each track. If youre looking for a new take on the Dolls, youre better off checking out Morrisseys brilliant cover of Human Being, released earlier this year ...
DeMauro SB, DAgostino JA, Bann C, Bernbaum J, Gerdes M, Bell EF, Carlo WA, DAngio CT, Das A, Higgins R, Hintz SR, Laptook AR, Natarajan G, Nelin L, Poindexter BB, Sanchez PJ, Shankaran S, Stoll BJ, Truog W, Van Meurs KP, Vohr B, Walsh MC, Kirpalani H. Developmental outcomes of very preterm infants with tracheostomies. J Pediatr. 2014 Jun; 164(6):1303-10.e2 ...
During the first two games of the season, Virginia special teams coordinator Anthony Poindexter got the sense that his kickoff returners werent quite buying in to the message they were being preached. He saw them moving side to side, waiting...
Ebersbach, Gitte and Briegel, Ariane and Jensen, Grant J. et al. (2008) A Self-Associating Protein Critical for Chromosome Attachment, Division, and Polar Organization in Caulobacter. Cell, 134 (6). pp. 956-968. ISSN 0092-8674. PMCID PMC2614312. https://resolver.caltech.edu/CaltechAUTHORS:EBEc08 ...
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Cell signaling governs basic cellular activities and coordinates cell actions. Cell signaling may help diseases to be treated effectively and cures created.
Looking for online definition of Caulobacter crescentus in the Medical Dictionary? Caulobacter crescentus explanation free. What is Caulobacter crescentus? Meaning of Caulobacter crescentus medical term. What does Caulobacter crescentus mean?
Lee Rannals for redOrbit.com - Your Universe Online. Former astronaut Alan G. Poindexter, who flew on space shuttle missions to the International Space Station, died on Sunday.. Florida Fish and Wildlife Conservation Commission officials said 51-year-old Poindexter died while riding on a jet ski with his 22-year-old son.. According to a report by the Pensacola News Journal, Zachary Poindexter, his other 26-year-old son, ran into the rear of the other watercraft, knocking his father into the water.. Poindexter was pulled from the water and taken to the beach, where friends tried performing CPR, wildlife agency spokesman Stan Kirkland said.. He was flown to Baptist Hospital by helicopter, where he died shortly afterwards due to injuries, according to the Pensacola News Journal.. Zachary Poindexter and his brother Samuel were not injured during the accident.. Poindexter flew on two space flights to the ISS during his career with NASA. He piloted space shuttle Atlantis in February 2008 on mission ...
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Caulobacter crescentus is an oligotrophic alpha-proteobacterium with a complex cell cycle involving sessile-stalked and piliated, flagellated swarmer cells. Because the natural lifestyle of C. crescentus intrinsically involves a surface-associated, sessile state, we investigated the dynamics and control of C. crescentus biofilms developing on glass surfaces in a hydrodynamic system. In contrast to biofilms of the well-studied Pseudomonas aeruginosa, Escherichia coli, and Vibrio cholerae, C. crescentus CB15 cells form biphasic biofilms, consisting predominantly of a cell monolayer biofilm and a biofilm containing densely packed, mushroom-shaped structures. Based on comparisons between the C. crescentus strain CB15 wild type and its holdfast (hfsA; DeltaCC0095), pili (DeltapilA-cpaF::Omegaaac3), motility (motA), flagellum (flgH) mutants, and a double mutant lacking holdfast and flagellum (hfsA; flgH), a model for biofilm formation in C. crescentus is proposed. For both biofilm forms, the holdfast ...
TY - JOUR. T1 - Two independent spiral structures control cell shape in Caulobacter. AU - Dye, Natalie A.. AU - Pincus, Zachary. AU - Theriot, Julie A.. AU - Shapiro, Lucy. AU - Gitai, Zemer. PY - 2005/12/20. Y1 - 2005/12/20. N2 - The actin homolog MreB contributes to bacterial cell shape. Here, we explore the role of the coexpressed MreC protein in Caulobacter and show that it forms a periplasmic spiral that is out of phase with the cytoplasmic MreB spiral. Both mreB and mreC are essential, and depletion of either protein results in a similar cell shape defect. MreB forms dynamic spirals in MreC-depleted cells, and MreC localizes helically in the presence of the MreB-inhibitor A22, indicating that each protein can form a spiral independently of the other. We show that the peptidoglycan transpeptidase Pbp2 also forms a helical pattern that partially colocalizes with MreC but not MreB. Perturbing either MreB (with A22) or MreC (with depletion) causes GFP-Pbp2 to mislocalize to the division plane, ...
Antibiotic persistence is a transient phenotypic state during which a bacterium can withstand otherwise lethal antibiotic exposure or environmental stresses. In Escherichia coli, persistence is promoted by the HipBA toxin-antitoxin system. The HipA toxin functions as a serine/threonine kinase that inhibits cell growth, while the HipB antitoxin neutralizes the toxin. E. coli HipA inactivates the glutamyl-tRNA synthetase GltX, which inhibits translation and triggers the highly conserved stringent response. Although hipBA operons are widespread in bacterial genomes, it is unknown if this mechanism is conserved in other species. Here we describe the functions of three hipBA modules in the alpha-proteobacterium Caulobacter crescentus. The HipA toxins have different effects on growth and macromolecular syntheses, and they phosphorylate distinct substrates. HipA1 and HipA2 contribute to antibiotic persistence during stationary phase by phosphorylating the aminoacyl-tRNA synthetases GltX and TrpS. The stringent
About the last one, Poindexter observed that as a result of a national survey she conducted for her forthcoming book The News for Mobile First Consumers, she sees the rise of three types of mobile consumers: The mobile first consumers are the ones who use their smartphone for everything; the mobile specialists, who use their mobile devices for entertainment, and the Internet; and the mobile laggards, who use smartphones for communication (to check emails, etc.).. She considers the millennial generation to be people who are now in their 30s (born during the 1980s and 1990s). According to Poindexter, this generation is less likely to subscribe to news because they have been mocked and demeaned by the news media, which is why they dont have the best relationship with the news media.. Millennials are up to five times more likely to be on social media than to seek news, said Poindexter.. About her study and the millenials apparent lack of desire to be informed, Poindexter concluded that these ...
Cryoelectron microscope tomography (cryoEM) and a fluorescence loss in photobleaching (FLIP) assay were used to characterize progression of the terminal stages of Caulobacter crescentus cell division. Tomographic cryoEM images of the cell division site show separate constrictive processes closing first the inner membrane (IM) and then the outer membrane (OM) in a manner distinctly different from that of septum-forming bacteria. FLIP experiments had previously shown cytoplasmic compartmentalization (when cytoplasmic proteins can no longer diffuse between the two nascent progeny cell compartments) occurring 18 min before daughter cell separation in a 135-min cell cycle so the two constrictive processes are separated in both time and space. In the very latest stages of both IM and OM constriction, short membrane tether structures are observed. The smallest observed pre-fission tethers were 60 nm in diameter for both the inner and outer membranes. Here, we also used FLIP experiments to show that ...
Second messengers control a wide range of important cellular functions in eukaryotes and prokaryotes. Here we show that cyclic di-GMP, a global bacterial second messenger, promotes cell cycle progression in Caulobacter crescentus by mediating the specific degradation of the replication initiation inhibitor CtrA. During the G1-to-S-phase transition, both CtrA and its cognate protease ClpXP dynamically localize to the old cell pole, where CtrA is rapidly degraded. Sequestration of CtrA to the cell pole depends on PopA, a newly identified cyclic di-GMP effector protein. PopA itself localizes to the cell pole and directs CtrA to this subcellular site via the direct interaction with a mediator protein, RcdA. We present evidence that c-di-GMP regulates CtrA degradation during the cell cycle by controlling the dynamic sequestration of the PopA recruitment factor to the cell pole. Furthermore, we show that cell cycle timing of CtrA degradation relies on converging pathways responsible for substrate and ...
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Generation and filtering of gene expression noise by the bacterial cell cycle. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
My research interests include Bacterial Cell Cycle Regulation and Signal Transduction mechanisms.. 1. Bacterial Cell Cycle Regulation- Epigenetic mechanisms regulating various physiological activities in the prokaryotic cells are increasingly being appreciated. Methylation of specific bases of the DNA molecule by methyltransferases is the most common epigenetic modification observed in the bacterial cells. This modification of nucleotides adds another level of regulation at the transcription; furthermore, it has a fundamental role in the cell physiological processes such as DNA replication, DNA mismatch repair, and virulence mechanisms in many pathogens. Among the prokaryotic DNA methyltransferases, Dam expressed among the Gammaproteobacteria is the most intensively studied. In contrast to Dam, another DNA methyltransferase CcrM (Cell Cycle Regulated Methylase) has been described in the Alphaproteobacteria, where it plays an important role in the cell cycle regulation of Caulobacter crescentus ...
In Caulobacter crescentus, the temporal and spatial expression of late flagellar genes is regulated by the 54 transcriptional activator, FlbD. Genetic experiments have indicated that the trans-acting factor FliX regulates FlbD in response to the progression of flagellar assembly, repressing FlbD activity until an early flagellar basal body structure is assembled. Following assembly of this structure, FliX is thought to function as an activator of FlbD. Here we have investigated the mechanism of FliX-mediated regulation of FlbD activity. In vitro transcription experiments showed that purified FliX could function as a repressor of FlbD-activated transcription. Transcription activated by a gain-of-function mutant of FlbD (FlbD-1204) that is active in vivo in the absence of an early flagellar structure, was resistant to the repressive effects of FliX. DNA binding studies showed that FliX inhibited the interaction of wild-type FlbD with enhancer DNA but did not effect FlbD-catalysed ATPase ...
16. The ADA and Rehabilitation Act regulations also assist courts in determining whether a particular endeavor may properly be considered a major life activity. Rather than enunciating a general principle for determining what is and is not a major life activity, [these] regulations instead provide a representative list, defining [the] term to include functions such as caring for ones self, performing manual tasks, walking, seeing, hearing, speaking, breathing, learning, and working. Bragdon, 524 U.S. at ----, 118 S.Ct. at 2205 (quoting 45 C.F.R. § 84.3(j)(2)(ii) (1997); 28 C.F.R. § 41.31(b)(2) (1997)); see also 29 C.F.R. § 1630.2(i) (1998). As the Department of Health and Human Services asserts, This list is not exhaustive. For example, other major life activities include, but are not limited to, sitting, standing, lifting, and reaching. 29 C.F.R. pt. 1630, app. at 347 (1998). Finally, if the asserted major life activity is not one of the examples listed above, under the plain meaning ...
Jensen, E. A. , Dysart, K. C. , Gantz, M. G. , Carper, B. , Higgins, R. D. , Keszler, M. , Laughon, M. M. , Poindexter, B. B. , Stoll, B. J. , Walsh, M. C. , Schmidt, B. , Caplan, M. S. , Polin, R. A. , Laptook, A. R. , Keszler, M. , Hensman, A. M. , Basso, K. M. , Vieira, E. , Little, E. , Fanaroff, A. A. & 136 others Hibbs, A. M., Newman, N. S., Truog, W. E., Kilbride, H. W., Pallotto, E. K., Gauldin, C., Holmes, A., Johnson, K., Knutson, A., Schibler, K., Donovan, E. F., Alexander, B., Grisby, C., Hessling, J., Fischer, E. E., Jackson, L. D., Kirker, K., Muthig, G., Goldberg, R. N., Cotten, C. M., Fisher, K. A., Auten, K. J., Foy, K. A., Grimes, S., Finkle, J., Laughon, M. M., Bose, C. L., Bernhardt, J., Bose, G., Carlton, D. P., Hale, E. C., Archer, S. W., Poindexter, B. B., Sokol, G. M., Wilson, L. D., Herron, D. E., Sánchez, P. J., Nelin, L. D., Jadcherla, S. R., Luzader, P., Fortney, C. A., Besner, G. E., Parikh, N. A., Das, A., Wallace, D., Auman, J. O. D., Crawford, M. M., Petrie ...
The deaths of nearby relatives have a curious effect on the bacterium Caulobacter crescentus -- surviving cells lose their stickiness. Indiana University Bloomington biologists report in an upcoming issue of Molecular Microbiology that exposure to the extracellular DNA (eDNA) released by dying neighbors stops the sticky holdfasts of living Caulobacter from adhering to surfaces, preventing cells from joining bacterial biofilms. Less sticky cells are more likely to escape established colonies, out to where conditions may be better.
An essential GTPase which binds GTP, GDP and ppGpp with moderate affinity (with a twofold preference for GDP over GTP), shows high guanine nucleotide exchange rate constants for both nucleotides, and has a relatively low GTP hydrolysis rate. Deletion of the 159 N-terminal residues makes a protein that is non-functional in vivo but which retains nucleotide binding and GTPase activity. Required for cell cycle progression from G1 to S phase and for DNA replication.
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By William Margolin | The division of one cell into two daughter cells is the crux of biological reproduction. But how do cells determine where along their dimensions division will occur? For bacteria, the best-studied species for basic biology, including cytokinesis, are the old standbys Escherichia coli, Bacillus subtilis, and Caulobacter crescentus, mainly because of their easy cultivation…
Middleton C.A., J.L. Pate. 1976. Isolation and partial characterization of some new bacteriophages active against Asticcacaulis strains. Int. J. Syst. Bacteriol. 26: 269-277. ...
Caulobacter, Mycobacterium). Many bacteria that lack MinC carry alternative proteins that can position their Z-ring. MinC is ...
... (Caulobacter response to famine RNA) is a family of non-coding RNAs found in Caulobacter crescentus. CrfA is expressed ... CrfA RNA is one of only 8 putative ncRNAs conserved in the closely related Caulobacter sp. K31. CrfA was found to be ... Landt SG, Abeliuk E, McGrath PT, Lesley JA, McAdams HH, Shapiro L (May 2008). "Small non-coding RNAs in Caulobacter crescentus ... Hinz AJ, Larson DE, Smith CS, Brun YV (February 2003). "The Caulobacter crescentus polar organelle development protein PodJ is ...
Hardwick SW, Chan VS, Broadhurst RW, Luisi BF (March 2011). "An RNA degradosome assembly in Caulobacter crescentus". Nucleic ...
2001). "Complete genome sequence of Caulobacter crescentus". Proc. Natl. Acad. Sci. U.S.A. 98 (7): 4136-41. Bibcode:2001PNAS... ...
nov., a novel predator of Caulobacter crescentus". International Journal of Systematic and Evolutionary Microbiology. 63 (1): ...
Bacillus subtilis Caulobacter crescentus Madigan, Michael T.; Martinko, John M. (2006). Brock Biology of Microorganisms (11th ...
To influence the shape of the Caulobacter cells, the helices of crescentin filaments associate with the cytoplasmic side of the ... Crescentin is necessary for both shapes of the Caulobacter prokaryote (vibroid/crescent-shape and helical shape, which it may ... This protein family is found in Caulobacter and Methylobacterium. Crescentin was recently discovered by Christine Jacobs-Wagner ... Margolin W (March 2004). "Bacterial shape: concave coiled coils curve caulobacter". Current Biology. 14 (6): R242-4. doi: ...
as the type species, and emended description of the genera Brevundimonas and Caulobacter" (PDF). Int. J. Syst. Bacteriol. 49 (3 ... "Phylogeny and polyphasic taxonomy of Caulobacter species. Proposal of Maricaulis gen. nov. with Maricaulis maris (Poindexter) ...
Poindexter, JS (Sep 1964). "BIOLOGICAL PROPERTIES AND CLASSIFICATION OF THE CAULOBACTER GROUP". Microbiol. Mol. Biol. Rev. 28 ( ... Asticcacaulis biprosthecum is a stalked bacterial species phylogenetically closely related to the species Caulobacter ...
The type order is the Caulobacterales, comprising stalk-forming bacteria such as Caulobacter. The mitochondria of eukaryotes ... Caulobacter, Rickettsia, Wolbachia, etc. Betaproteobacteria: Bordetella, Ralstonia, Neisseria, Nitrosomonas, etc. ...
Braz VS, Marques MV (October 2005). "Genes involved in cadmium resistance in Caulobacter crescentus". FEMS Microbiology Letters ...
The typespecies Caulobacter gives its name also to the recently proposed subclass, the Caulobacteridae, which includes the ... Caulobacteraceae includes the genera Asticcacaulis, Brevundimonas, Phenylobacterium and Caulobacter. ...
Caulobacter Oligotrophic Flagella Pilus Poindexter, Jeanne S. Dimorphic Prosthecate Bacteria: The Genera Caulobacter, ... One notable group of prosthecates is the genus Caulobacter. Prosthecates are generally chemoorganotrophic aerobes that can grow ...
The process of the Caulobacter cell cycle also show similarities to stem cell division, in which two distinct cells arise, one ... In each cell cycle, Caulobacter divides asymmetrically into two daughters. One, the swarmer cell, has a tail-like flagellum ... Examining the cell cycle control logic of Caulobacter as a state machine leads to understanding of bacterial cell cycle ... Rather than containing an evenly dispersed mixture of proteins, the single celled Caulobacter resembles a highly organized ...
Enterobacteria, caulobacter, pseudomonas, and acinetobacter serve as the natural host. The family has two genera, both of which ... Bacteria, including enterobacteria, caulobacter, pseudomonas, and acinetobacter serve as natural hosts for these bacteriophages ...
A well established example of bacterial aging is Caulobacter crescentus. This bacteria begins its life as a motile swarmer cell ... such as Caulobacter crescentus, show signs of replicative aging. The results for symmetrically dividing bacteria are more ...
Stahl D. A., Key R., Flesher B., and Smit J.; (1992); 'The phylogeny of marine and freshwater Caulobacter reflects their ...
This was verified with the resolution of the crystal structure of the DGC PleD from Caulobacter crescentus in complex with c-di ... The GGDEF domain was first identified in the regulatory protein, PleD of the bacterium Caulobacter crescentus. It was later ... In the cell cycle of Caulobacter crescentus, DGC PleD is known to control pole morphogenesis. In Pseudomonas fluorescens DGC ... Skerker JM, Laub MT (April 2004). "Cell-cycle progression and the generation of asymmetry in Caulobacter crescentus". Nature ...
The bacterium Caulobacter crescentus contains a third protein, crescentin, that is related to the intermediate filaments of ... "Multiple large filament bundles observed in Caulobacter crescentus by electron cryotomography". Molecular Microbiology. 62 (1 ...
Goley E.D., Yeh Y.C., Hong S.H., Fero M.J., Abeliuk E., McAdams H.H., and Shapiro L. (2011) Assembly of the Caulobacter cell ... Landt S.G., Abeliuk E., McGrath P.T., Lesley J.A., McAdams H.H., Shapiro L. (2008) Small non-coding RNAs in Caulobacter ... The global regulatory architecture of transcription during the Caulobacter cell cycle., PLoS Genet. Abeliuk E, Christen B, Fero ... Regulatory Response to Carbon Starvation in Caulobacter crescentus. PLoS One. ...
More work can be found that focus on modeling a particular cellular process such as the growth cycle of Caulobacter crescentus ... Li, S; Brazhnik, P; Sobral, B; Tyson, JJ (2009). "Temporal Controls of the Asymmetric Cell Division Cycle in Caulobacter ...
Some bacteria have complex life cycles involving the production of stalks and appendages (e.g. Caulobacter) and some produce ...
... proposal of Caulobacter mirabilis sp. nov., Phenylobacterium haematophilum sp. nov. and Phenylobacterium conjunctum sp. nov., ...
socAB is a type VI toxin-antitoxin system that was discovered in Caulobacter crescentus. The antitoxin, SocA, promotes ...
Caulobacter crescentus - a bacterium that divides into two distinct cells used to study cellular differentiation. Mycoplasma ...
It has a large polar or sub-polar filiform prostheca very similar to that of Caulobacter. In addition to having a nutritional ...
EcoDam from E. coli and CcrM from Caulobacter crescentus are well characterized members of these family. More recently, CamA ...
... superresolution colocalization of intracellular protein superstructures and the cell surface in live Caulobacter crescentus". ...
In the less common type, such as the Bacillus subtilis sporulation factor Spo0B or the Caulobacter crescentus protein ChpT, the ... as in the case of the Caulobacter crescentus ChpT HPt involved in cell cycle regulation, or, alternatively, pathways in which ... an essential regulator of stalk biogenesis in Caulobacter crescentus". Journal of Molecular Biology. 390 (4): 686-98. doi: ...
Valencia EY, Braz VS, Guzzo C, Marques MV (April 2013). "Two RND proteins involved in heavy metal efflux in Caulobacter ...
The opposing roles of the Caulobacter DnaA and CtrA proteins are essential to the tight control of Caulobacter chromosome ... C. crescentus is synonymous with Caulobacter vibrioides. The Caulobacter CB15 genome has 4,016,942 base pairs in a single ... The taxon is more properly known as Caulobacter vibrioides (Henrici and Johnson 1935). Caulobacter is an important model ... The Caulobacter stalked cell stage provides a fitness advantage by anchoring the cell to surfaces to form biofilms and or to ...
Complete genome sequence of Caulobacter crescentus. William C. Nierman, Tamara V. Feldblyum, Michael T. Laub, Ian T. Paulsen, ... Complete genome sequence of Caulobacter crescentus. William C. Nierman, Tamara V. Feldblyum, Michael T. Laub, Ian T. Paulsen, ... Complete genome sequence of Caulobacter crescentus. William C. Nierman, Tamara V. Feldblyum, Michael T. Laub, Ian T. Paulsen, ... Comparison of the Caulobacter proteome to that of other species reveals that there are more matches to the P. aeruginosa ...
The Caulobacter Phage Holin (CauHol) Family (TC# 1.E.47) consists of several putative holins of 157 to 159 amino acyl residues ... The Caulobacter Phage Holin (CauHol) Family". TCDB. Retrieved 2016-03-29. Biology portal As of this edit, this article uses ... aas) in length that exhibit 2 transmembrane segments (TMSs). They derive from phage specific for Caulobacter species. These ... content from "1.E.47 The Caulobacter Phage Holin (CauHol) Family", which is licensed in a way that permits reuse under the ...
Make research projects and school reports about Caulobacter easy with credible articles from our FREE, online encyclopedia and ... Caulobacter. Caulobacter crescentus is a Gram-negative rod-like bacterium that inhabits fresh water. It is noteworthy ... Caulobacter A genus of Gram-negative bacteria not assigned to any taxonomic family. The cells undergo a life cycle that is ... Caulobacter crescentus can be grown in the laboratory so that all the bacteria in the population undergoes division at the same ...
The evolution of stalk positioning in the Caulobacter cladeEdit. Diverse positioning of the stalks. Caulobacter crescentus ( ... Caulobacter agingEdit. Caulobacter was the first asymmetric bacterium shown to age. Reproductive senescence was measured as the ... The opposing roles of the Caulobacter DnaA and CtrA proteins are essential to the tight control of Caulobacter chromosome ... The Caulobacter CB15 genome has 4,016,942 base pairs in a single circular chromosome encoding 3,767 genes.[7] The genome ...
1984) Caulobacter crescentus flagellar filament has a right-handed helical form. J Mol Biol 173(1):125-130. ... Helical motion of the cell body enhances Caulobacter crescentus motility. Bin Liu, Marco Gulino, Michael Morse, Jay X. Tang, ... Caulobacter strain CB15 Δpilin (YB375), which lacks pili, was grown in peptone yeast extract medium (0.2% Bacto Peptone, 0.1% ... Making use of the crescent shape of the cell body of Caulobacter, we are able to reconstruct the 3D cell kinematics from the ...
hypothetical protein Cseg_0582 [Caulobacter segnis ATCC 21756]. * Record removed. The sequence YP_003591712 is 100% identical ...
Biotin metabolism - Caulobacter segnis [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show description , User ...
Walker S.G., Smit J. (1993) Attachment of the S-Layer of Caulobacter crescentus to the Cell Surface. In: Beveridge T.J., Koval ... Smit, J., Grano, D.A., Glaser, R.M., and Agabian, N., 1981, Periodic surface array in Caulobacter crescentus: fine structure ... Gilchrist, A., Fisher, J.A., and Smit, J., 1992, Nucleotide sequence analysis of the gene encoding the Caulobacter crescentus ... Smit, J., Engelhardt, H., Volker, S., Smith, S.H., and Baumeister, W., 1992, The Slayer of Caulobacter crescentus: Three- ...
The Caulobacter crescentus flagellar gene, fliX, encodes a novel trans-acting factor that couples flagellar assembly to ... The first flagellar assembly checkpoint of Caulobacter crescentus couples assembly of the early class II components of the ...
Caulobacter daughter cells have two very different forms. One daughter is a mobile "swarmer" cell that has a single flagellum ... From left to right: i) The number of proteins in the reference proteome of Caulobacter crescentus, ii) the total number of ... The bar plot shows the coverage for every protein in the reference proteome of Caulobacter crescentus for which there is at ... Caulobacter crescentus is a Gram-negative, oligotrophic bacterium widely distributed in freshwater lakes and streams. ...
... and cell division in stalked cells of the α-proteobacterium Caulobacter crescentus. The model is formulated in terms of ... for the spatial asymmetry of Caulobacter reproduction (swarmer cells as well as stalked cells), the correlation of cell growth ... nonlinear ordinary differential equations for the major cell cycle regulatory proteins in Caulobacter: CtrA, GcrA, DnaA, CcrM, ... Caulobacter crescentus Is the Subject Area "Caulobacter crescentus" applicable to this article? Yes. No. ...
Caulobacter crescentus is a gram-negative bacterium that can utilize xylose as a substrate using the Weimberg pathway, which ... Characterization of the Weimberg Pathway in Caulobacter crescentus. Henrik Almqvist 1,* , Sara Jonsdottir Glaser 1,†. ... Caulobacter crescentus is a gram-negative bacterium that can utilize xylose as a substrate using the Weimberg pathway, which ... Keywords: Caulobacter crescentus; Weimberg pathway; xylose; arabinose; physiological characterization; enzyme activity ...
Caulobacter vibrioides CB15. Mutation(s): 0 Gene Names: Itgav, CC_2357. Find proteins for Q9A5U0 (Caulobacter vibrioides ( ... Structure of a GH39 Beta-xylosidase from Caulobacter crescentus. Polo, C.C., Santos, C.R., Correa, J.M., Simao, R.C.G., Seixas ... Structure of a GH39 Beta-xylosidase from Caulobacter crescentus. *DOI: 10.2210/pdb4M29/pdb ...
Caulobacters RsaA protein was adapted to make 2 recombinant proteins of the pilus epitope (termed adhesintopes) using the PE ... Caulobacter crescentus in Biotechnology. From MicrobeWiki, the student-edited microbiology resource. Revision as of 05:51, 15 ... Caulobacter crescentus is a non-pathogenic, aquatic, Gram-negative bacterium.[9] One of its most distinguishing characteristics ... 2. Awram, P., Smit, J. "The Caulobacter crescentus paracrystalline S-layer protein is secreted by an ABC transporter (type I) ...
Citrate cycle (TCA cycle) - Caulobacter segnis [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
Caulobacter vibrioides (Caulobacter crescentus). Caulobacter sp. 410. Caulobacter flavus. Caulobacter sp. FWC26. 231. UniRef90_ ... Caulobacter crescentus (strain ATCC 19089 / CB15). 231. UniRef100_B8H627. Cluster: Ribonuclease 3. 2. ... sp,B8H627,RNC_CAUCN Ribonuclease 3 OS=Caulobacter crescentus (strain NA1000 / CB15N) OX=565050 GN=rnc PE=3 SV=1 ... cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter › ...
... Author(s). Gora, Kasia G. (Kasia Gabriela) ... In this thesis, I use the model system Caulobacter crescentus to examine the regulation of CtrA, the essential transcription ... factor at the core of the Caulobacter cell cycle regulatory network. CtrA regulates the activity of approximately 100 cell ...
... images of small bacterial cells was developed and used to identify chemoreceptor arrays in cryotomograms of intact Caulobacter ... Location and Architecture of the Caulobacter Crescentus Chemoreceptor Array Mol Microbiol. 2008 Jul;69(1):30-41. doi: 10.1111/j ... The arrays were always found on the convex side of the cell, further demonstrating that Caulobacter cells maintain dorsal/ ... images of small bacterial cells was developed and used to identify chemoreceptor arrays in cryotomograms of intact Caulobacter ...
Caulobacter sp. 410. Caulobacter flavus. Caulobacter sp. FWC2. Caulobacter sp. B11. Caulobacter sp. X. Caulobacter sp. FWC26. ... Caulobacter crescentus (strain ATCC 19089 / CB15). Caulobacter vibrioides (Caulobacter crescentus). ... Caulobacter segnis (strain ATCC 21756 / DSM 7131 / JCM 7823 / NBRC 15250 / LMG 17158 / TK0059) (Mycoplana segnis). Caulobacter ... Caulobacter crescentus (strain ATCC 19089 / CB15). 358. UniRef100_B8GZ33. Cluster: Modification methylase CcrMI. 2. ...
Caulobacter strain history.The history of these Caulobacter strains was pieced together from information gathered from personal ... The Genetic Basis of Laboratory Adaptation in Caulobacter crescentus Melissa E. Marks, Cyd Marie Castro-Rojas, Clotilde Teiling ... The Genetic Basis of Laboratory Adaptation in Caulobacter crescentus Melissa E. Marks, Cyd Marie Castro-Rojas, Clotilde Teiling ... The Genetic Basis of Laboratory Adaptation in Caulobacter crescentus Melissa E. Marks, Cyd Marie Castro-Rojas, Clotilde Teiling ...
... and deactivation of the essential transcriptional regulator CtrA is necessary to drive cell cycle progression in Caulobacter ... Mutations that alter RcdA surface residues decouple protein localization and CtrA proteolysis in Caulobacter crescentus J Mol ... We assayed the ability of each RcdA variant to support CtrA proteolysis and polar protein localization in Caulobacter. Deletion ... and deactivation of the essential transcriptional regulator CtrA is necessary to drive cell cycle progression in Caulobacter ...
Title: Environmental Calcium Controls Alternate Physical States of the Caulobacter Surface Layer ... Published Article: Environmental Calcium Controls Alternate Physical States of the Caulobacter Surface Layer ...
ppGpp and Polyphosphate Modulate Cell Cycle Progression in Caulobacter crescentus. Cara C. Boutte, Jonathan T. Henry, Sean ... ppGpp and Polyphosphate Modulate Cell Cycle Progression in Caulobacter crescentus. Cara C. Boutte, Jonathan T. Henry, Sean ... ppGpp and Polyphosphate Modulate Cell Cycle Progression in Caulobacter crescentus. Cara C. Boutte, Jonathan T. Henry, Sean ... Caulobacter and Asticcaculis stalk bands as indicators of stalk age. J. Bacteriol. 178:3939-3948. ...
... Author(s). Chen, Yiyin Erin ... Caulobacter is an alpha-proteobacterium that always divides asymmetrically to generate two daughter cells that are ... In this work, I use the model organism Caulobacter crescentus to investigate how intracellular asymmetry within the mother cell ... The genetic separability of the spatial and temporal controls of replication in Caulobacter suggests that DnaA comprises an ...
... signals from the cytoplasm to the cell wall to regulate key developmental shape changes in the α-proteobacterium Caulobacter ... Caulobacter crescentus Is the Subject Area "Caulobacter crescentus" applicable to this article? Yes. No. ...
An image of slice 210 from the reconstructed volume of a tomographic data set from Caulobacter crescentus. This image has been ... Caulobacter cultures (strain 3724) were cryofixed using a cocktail of 2% Osmium tetroxide, 0.5% uranyl acetate in anhydrous ... An image of slice 210 from the reconstructed volume of a tomographic data set from Caulobacter crescentus. This image has been ... Lucy Shapiro, Harley McAdams (2012) CIL:40007, Caulobacter crescentus CB15. CIL. Dataset. https://doi.org/doi:10.7295/ ...
In Caulobacter crescentus, a bacterium known for studies of bacterial cell division cycle, the response regulator CpdR couples ... Control of proteolysis during the Caulobacter cell cycle occurs through proteins involved in a complex phosphosignaling network ... In Caulobacter crescentus, a bacterium known for studies of bacterial cell division cycle, the response regulator CpdR couples ... Control of proteolysis during the Caulobacter cell cycle occurs through proteins involved in a complex phosphosignaling network ...
What is Caulobacter crescentus? Meaning of Caulobacter crescentus medical term. What does Caulobacter crescentus mean? ... Looking for online definition of Caulobacter crescentus in the Medical Dictionary? Caulobacter crescentus explanation free. ... Caulobacter crescentus. Also found in: Encyclopedia, Wikipedia. Caulobacter crescentus. (kawl-ŏ-băk′tĕr krĕ-sĕn′tŭs) A single- ... Figure 2 shows that Caulobacter crescentus has regular ly higher C and lower A in the third position.. Global analysis of ...
... from Caulobacter crescentus CB15 at 2.83 A resolution (PSI Community Target, Shapiro) ... Caulobacter crescentus (strain ATCC 19089 / CB15). N/A. Find proteins for Q9A712 (Caulobacter crescentus (strain ATCC 19089 / ... Crystal structure of a putative outer membrane chaperone (OmpH-like) (CC_1914) from Caulobacter crescentus CB15 at 2.83 A ... Crystal structure of a putative outer membrane chaperone (OmpH-like) (CC_1914) from Caulobacter crescentus CB15 at 2.83 A ...
  • Due to this capacity to be physically synchronized, strain NA1000 has become the predominant experimental Caulobacter strain throughout the world. (wikipedia.org)
  • In 2010, the Caulobacter NA1000 strain was sequenced and all differences with the CB15 "wild type" strain were identified. (wikipedia.org)
  • Then, they reduced the native Caulobacter crescentus native Caulobacter NA1000 genome sequence real genome ( 4042929 bp ) to the 785,701-bp reduced synthetic genome. (preprints.org)
  • Comparing the transcriptional profiles of a Caulobacter crescentus CB15N (NA1000) wild-type to a SpoT null in carbon starvation. (omicsdi.org)
  • Caulobacter crescentus NA1000 possess two HME-RND proteins, and the aim of this work was to determine their involvement in the response to cadmium, zinc, cobalt and nickel, and to analyze the phylogenetic distribution and characteristic signatures of orthologs of these two proteins. (beds.ac.uk)
  • The Caulobacter CB15 genome has 4,016,942 base pairs in a single circular chromosome encoding 3,767 genes. (wikipedia.org)
  • CIL:40007, Caulobacter crescentus CB15. (cellimagelibrary.org)
  • Lucy Shapiro, Harley McAdams (2012) CIL:40007, Caulobacter crescentus CB15. (cellimagelibrary.org)
  • Copper-Zinc superoxide dismutase from Caulobacter crescentus CB15. (thefreedictionary.com)
  • Using a digital tracking microscope that provides both cell position and orientation, we have correlated the detailed motion of the cell body of a fast-swimming bacterium, Caulobacter crescentus , with its swimming motility. (pnas.org)
  • We have used this technique to study the motility of the uniflagellated bacterium Caulobacter crescentus and have found that each cell displays two distinct modes of motility, depending on the sense of rotation of the flagellar motor. (pnas.org)
  • The dimorphic bacterium Caulobacter crescentus has evolved marked phenotypic changes during its 50-year history of culture in the laboratory environment, providing an excellent system for the study of natural selection and phenotypic microevolution in prokaryotes. (asm.org)
  • Likewise, the aquatic bacterium Caulobacter crescentus has evolved marked phenotypic changes during the 50 years it has been cultured in the laboratory environment. (asm.org)
  • The researchers started the project by trying to understand why the bacterium Caulobacter crescentus produces two distinct types of cell - one with a flagellum (appendage) and the other without. (thefreedictionary.com)
  • Here, we demonstrate that the freshwater bacterium Caulobacter crescentus and related species transform into filamentous cells in response to conditions that commonly occur in their natural habitat as a result of algal blooms during the warm summer months. (asm.org)
  • Dynamics and control of biofilms of the oligotrophic bacterium Caulobacter crescentus. (stanford.edu)
  • However, even that does not compare to what the bacterium Caulobacter crescentus can do. (listverse.com)
  • A protein critical for cell constriction in the Gram-negative bacterium Caulobacter crescentus localizes at the division site through its peptidoglycan-binding LysM domains. (helmholtz-hzi.de)
  • In order to gain insight about the material properties of bacterial adhesins, we study the morphogenesis of the adhesive holdfast of the Gram negative bacterium Caulobacter crescentus . (biomedcentral.com)
  • Microarray analysis was used to examine gene expression in the freshwater oligotrophic bacterium Caulobacter crescentus during growth on three standard laboratory media, including peptone-yeast extract medium (PYE) and minimal salts medium with glucose or xylose as the carbon source. (scu.edu)
  • CAULOBACTERThe common waterborne bacterium Caulobacter crescentus reproduces asymmetrically. (amazonaws.com)
  • In the article, "Obstruction of Pilus Retraction Stimulates Bacterial Surface Sensing," a group of interdisciplinary researchers show that in the case of the bacterium Caulobacter crescentus , a model organism that splits its life between swimming and attaching to surfaces, physically blocking the retraction of its pili triggers the attachment to surfaces. (eurekalert.org)
  • From left to right: i) The number of proteins in the reference proteome of Caulobacter crescentus , ii) the total number of models, iii) the number of unique protein sequences for which at least one model is available and iv) a coverage bar plot is shown. (expasy.org)
  • Control of proteolysis during the Caulobacter cell cycle occurs through proteins involved in a complex phosphosignaling network. (umass.edu)
  • To test this hypothesis, we generated mutations in the Caulobacter crescentus obg gene ( cgtA C ) which, in Ras-like proteins, would result in either activating or dominant negative phenotypes. (umich.edu)
  • We sought to identify FtsZ-binding proteins that influence FtsZ function in Caulobacter crescentus. (princeton.edu)
  • Cell cycle progression and polar morphogenesis in Caulobacter crescentus are coordinated by the interplay of multiple proteins in time and space. (unibas.ch)
  • Here, we reconstitute a parS -dependent ParB spreading event using purified proteins from Caulobacter crescentus and show that CTP is required for spreading. (elifesciences.org)
  • The pathway of fatty acid utilization was investigated in Caulobacter crescentus. (yu.edu)
  • This report suggests that another outer membrane component, termed the S-layer-associated oligosaccharide (SAO), is the molecule responsible for the attachment of RsaA, the S-layer protein of Caulobacter crescentus , to the cell surface. (springer.com)
  • The bar plot shows the coverage for every protein in the reference proteome of Caulobacter crescentus for which there is at least one model. (expasy.org)
  • We assayed the ability of each RcdA variant to support CtrA proteolysis and polar protein localization in Caulobacter. (nih.gov)
  • We used high-throughput PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in live Caulobacter crescentus. (epfl.ch)
  • Here, we identify an atypical two-protein bacteriocin in the α-proteobacterium Caulobacter crescentus that is retained on the surface of producer cells where it mediates cell contact-dependent killing. (elifesciences.org)
  • These aggregates can drive contact-dependent killing of other organisms, or Caulobacter cells not producing the CdzI immunity protein. (elifesciences.org)
  • Caulobacter crescentus nucleoid: analysis of sedimentation behavior and protein composition during the cell cycle. (microbiologyresearch.org)
  • Here, we explore the role of the coexpressed MreC protein in Caulobacter and show that it forms a periplasmic spiral that is out of phase with the cytoplasmic MreB spiral. (princeton.edu)
  • We have solved the X-ray crystal structure of the RNA chaperone protein Hfq from the alpha-proteobacterium Caulobacter crescentus to 2.15 Å resolution, resolving the conserved core of the protein and the entire C-terminal domain (CTD). (cam.ac.uk)
  • Here, using genome-wide methods and microscopy of single cells, we show that Caulobacter SMC is recruited to the centromeric parS site and that SMC-mediated arm alignment depends on the chromosome-partitioning protein ParB. (semanticscholar.org)
  • The Caulobacter crescentus Homolog of DnaA (HdaA) Also Regulates the Proteolysis of the Replication Initiator Protein DnaA. (illumina.com)
  • Three-dimensional superresolution colocalization of intracellular protein superstructures and the cell surface in live Caulobacter crescentus. (mendeley.com)
  • Here, we reconstitute a parS -dependent ParB spreading on DNA in real-time, using a label-free purified protein from Caulobacter crescentus . (elifesciences.org)
  • Caulobacter cultures (strain 3724) were cryofixed using a cocktail of 2% Osmium tetroxide, 0.5% uranyl acetate in anhydrous acetone following this time and temperature schedule: -90 Celsius for 72 hours, raised 2 Celsius per hour for 15 hours, held at -60 Celsius for 12 hours, raised 5 Celsius per hour for 12 hours, held at -20 Celsius. (cellimagelibrary.org)
  • The taxonomy of Caulobacter has relied primarily upon morphological criteria: A strain that visually appeared to be a member of the Caulobacter has generally been called one without challenge. (microbiologyresearch.org)
  • Complete genome sequence of a wild-type isolate of Caulobacter vibrioides strain CB2. (pacb.com)
  • The complete genome of Caulobacter vibrioides strain CB2 consists of a 4,123,726-bp chromosome, a GC content of 67.2%, and 3,896 coding DNA sequences. (pacb.com)
  • As determined by analyses of 16S rRNA gene sequences, the prosthecate strain FWC 38T was affiliated to the alphaproteobacterial genus Caulobacter, with Caulobacter henricii (96.8 %) and Caulobacter fusiformis (96.8 %) as its closest relatives. (ox.ac.uk)
  • Polarity of gene transfer in Caulobacter. (microbiologyresearch.org)
  • This will allow us to study the impact of horizontal gene transfer on caulobacter genomes. (pacb.com)
  • Here, we examine if selection acts on G+C contents in Caulobacter crescentus and Pseudomonas aeruginosa , which both have very G+C-rich genomes, by testing whether the expression of gene variants that differ only in their base compositions at synonymous sites affects cellular growth rates. (g3journal.org)
  • GapR is an α‐proteobacterial NAP that affects growth, division, DNA replication, and global gene expression in Caulobacter crescentus . (embopress.org)
  • However, our studies show that it likely does not play such a role in Caulobacter as depleting PhoU has no significant effect on PhoB-dependent gene expression. (datamed.org)
  • Instead of dividing two form two identical daughter cells as other bacteria do (a process termed binary division), Caulobacter crescentus undergoes what is termed symmetric division. (encyclopedia.com)
  • Caulobacter crescentus can be grown in the laboratory so that all the bacteria in the population undergoes division at the same time. (encyclopedia.com)
  • The genetic separability of the spatial and temporal controls of replication in Caulobacter suggests that DnaA comprises an ancient and phylogenetically widespread control module for replication in almost all bacteria while CtrA developed later in c-proteobacteria and was recruited to enforce replicative asymmetry in daughter cells. (mit.edu)
  • The genus Caulobacter is composed of prosthecate bacteria often specialized for oligotrophic environments. (microbiologyresearch.org)
  • Partially purified bacterial extracts were prepared from segregated stalked and swarmer bacteria of Caulobacter crescentus and assayed for ATP-dependent deoxyribonuclease activity. (microbiologyresearch.org)
  • Like many bacteria, Caulobacter responds to phosphate limitation through a conserved two-component signaling pathway called PhoR-PhoB, but the direct regulon of PhoB in this organism is unknown. (datamed.org)
  • Caulobacter crescentus differentiates from a motile, foraging swarmer cell into a sessile, replication-competent stalked cell during its cell cycle. (asm.org)
  • Chromosome replication in Caulobacter crescentus growing in a nutrient broth. (microbiologyresearch.org)
  • Using genomic techniques, quantitative cell imaging, and mathematical modeling, our study in Caulobacter crescentus identifies a novel NAP (GapR) whose activity over the cell cycle is shaped by DNA replication. (embopress.org)
  • Summary: When Caulobacter crescentus enters S-phase the replication initiation inhibitor CtrA dynamically positions to the old cell pole to be degraded by the polar ClpXP protease. (elsevier.com)
  • Caulobacter is an important model organism for studying the regulation of the cell cycle, asymmetric cell division, and cellular differentiation. (wikipedia.org)
  • In this work, I use the model organism Caulobacter crescentus to investigate how intracellular asymmetry within the mother cell is translated into the formation of two developmentally distinct daughter cells. (mit.edu)
  • Caulobacter crescentus is a model organism for the study of asymmetric division and cell type differentiation, as its cell division cycle generates a pair of daughter cells that differ from one another in their morphology and behavior. (ginsim.org)
  • The described species of the genus Caulobacter formed a paraphyletic group with Caulobacter henricii , Caulobacter fusiformis, Caulobacter vibrioides and Mycoplana segnis (Caulobacter segnis comb, nov.) belonging to Caulobacter sensu stricto. (microbiologyresearch.org)
  • Here we describe the functions of three hipBA modules in the alpha-proteobacterium Caulobacter crescentus. (chalmers.se)
  • Periodic activation and deactivation of the essential transcriptional regulator CtrA is necessary to drive cell cycle progression in Caulobacter crescentus. (nih.gov)
  • Transcriptional profiling of Caulobacter crescentus during growth on complex and minimal media. (helmholtz-hzi.de)
  • Transcriptional Profiling of Caulobacter crescentus during Growth on C" by Craig Stephens, Alison K. Hottes et al. (scu.edu)
  • The complete genome sequence of Caulobacter crescentus was determined to be 4,016,942 base pairs in a single circular chromosome encoding 3,767 genes. (pnas.org)
  • Caulobacter does all that with less than 4,000 genes, allowing full genome-wide studies of a single differentiating cell. (pnas.org)
  • The first flagellar assembly checkpoint of Caulobacter crescentus couples assembly of the early class II components of the basal body complex to the expression of class III and IV genes, which encode extracytoplasmic structures of the flagellum. (ingentaconnect.com)
  • 2019), authors r ebuilt the essential genome of Caulobacter crescentus through the process of chemical synthesis rewriting and studied the genetic information content at the level of its essential genes. (preprints.org)
  • Cloning vectors for studies of Caulobacter crescentus genes should be transferable between Escherichia coli and C. crescentus since a transformation system has not been developed for C. crescentus. (elsevier.com)
  • The ordered assembly of the Caulobacter crescentus flagellum is accomplished in part through the organization of the flagellar structural genes in a regulatory hierarachy of four classes. (umn.edu)
  • Analysis of the fliX- flgI intergenic region revealed an arrangement of cis-acting elements similar to that of another set of Caulobacter class II and class III flagellar genes, fliL-flgF, that is also divergently transcribed. (umn.edu)
  • The temporal and spatial expression of late flagellar genes in Caulobacter crescentus is activated by the transcription factor FlbD and its partner trans-acting factor FliX. (beds.ac.uk)
  • Caulobacter strains bearing mutations in fliX are non-motile and do not transcribe class III and IV flagellar genes. (beds.ac.uk)
  • The PhoB regulon is comprised primarily of genes known or predicted to help Caulobacter scavenge for and import inorganic phosphate, including 15 different membrane transporters. (datamed.org)
  • Marker nucleotides within the 16S rRNA genes were determined for the genera Asticcacaulis, Brevundimonas, Caulobacter and Phenylobacterium and the description of the genus Phenylobacterium is emended. (ox.ac.uk)
  • All mutants were successfully expressed in both wild-type and ΔfliX Caulobacter strains. (beds.ac.uk)
  • Physiological and biochemical tests allowed genotypic and phenotypic differentiation of the novel strains from all hitherto recognized species of the genera Caulobacter and Phenylobacterium. (ox.ac.uk)
  • The strains therefore represent novel species, for which the names Caulobacter mirabilis sp. (ox.ac.uk)
  • They derive from phage specific for Caulobacter species. (wikipedia.org)
  • Phylogeny and polyphasic taxonomy of Caulobacter species. (microbiologyresearch.org)
  • The halophilic species Caulobacter maris and Caulobacter halobacteroides are different from these two genera and form the genus Maricaulis gen. nov. with Maricaulis maris as the type specis Caulobacter leidyia was observed to cluster with species of the genus Sphingomonas. (microbiologyresearch.org)
  • Caulobacter crescentus displays a dimorphic life cycle, beginning with a flagellated and chemotactic swarmer cell that neither grows nor replicates its chromosome. (asm.org)
  • p. 731 , published online 24 October) analyzed the structure of the circular chromosome in the prokaryote Caulobacter crescentus by using chromosome conformation capture and deep-sequencing. (sciencemag.org)
  • Analysis of Hi-C data and polymer modeling indicates that the Caulobacter crescentus chromosome consists of multiple, largely independent spatial domains that are probably composed of supercoiled plectonemes arrayed into a bottle brush-like fiber. (sciencemag.org)
  • Caulobacter crescentus contains a single chromosome that is replicated once during a defined period in the cell cycle. (scripps.edu)
  • In Caulobacter crescentus, SMC is required to align the left and the right arms of the chromosome that run in parallel down the long axis of the cell. (semanticscholar.org)
  • Caulobacter requires a dedicated mechanism to initiate chromosome segregation. (semanticscholar.org)
  • The taxon is more properly known as Caulobacter vibrioides (Henrici and Johnson 1935). (wikipedia.org)
  • Caulobacter crescentus is a gram-negative bacterium that can utilize xylose as a substrate using the Weimberg pathway, which converts xylose to α -ketoglutarate in five steps without carbon loss. (mdpi.com)
  • Then, a new route for glycolate production was established in E . coli by introducing NAD + -dependent xylose dehydrogenase ( xdh ) and xylonolactonase ( xylC ) from Caulobacter crescentus . (springer.com)
  • This work demonstrates that a new enzymatic xylose quantification method, based on the activity of xylose dehydrogenase from Caulobacter crescentus , represents an excellent alternative to the manual phloroglucinol reaction. (hindawi.com)
  • Here, we dissect the c-di-GMP network of Caulobacter crescentus to establish a global and quantitative view of c-di-GMP dependent processes in this organism. (harvard.edu)
  • The Caulobacter cell cycle regulatory system controls many modular subsystems that organize the progression of cell growth and reproduction. (wikipedia.org)
  • Cryoelectron microscope tomography (cryoEM) and a fluorescence loss in photobleaching (FLIP) assay were used to characterize progression of the terminal stages of Caulobacter crescentus cell division. (meta.org)
  • Mutations in highly conserved regions of FliX could severely affect the recognition between FliX and FlbD and hence interrupt the normal progression of flagellar synthesis and other developmental events in Caulobacter . (beds.ac.uk)
  • The arrays were always found on the convex side of the cell, further demonstrating that Caulobacter cells maintain dorsal/ventral as well as anterior/posterior asymmetry. (nih.gov)
  • Caulobacter crescentus is a Gram-negative, oligotrophic bacterium widely distributed in fresh water lakes and streams. (wikipedia.org)
  • Caulobacter crescentus is an oligotrophic alpha-proteobacterium with a complex cell cycle involving sessile-stalked and piliated, flagellated swarmer cells. (stanford.edu)
  • Autolysis of Caulobacter crescentus grown in the presence of glycine. (helmholtz-hzi.de)
  • This study is measuring the steady-state levels of mRNA in wild-type Caulobacter crescentus grown in M2 defined medium containing either ammonium or nitrate as the sole nitrogen source. (omicsdi.org)
  • Caulobacter daughter cells have two very different forms. (wikipedia.org)
  • Detailed study of the molecular development of these cells as they progress through the cell cycle has enabled researchers to understand Caulobacter cell cycle regulation in great detail. (wikipedia.org)
  • A new method for recording both fluorescence and cryo-EM images of small bacterial cells was developed and used to identify chemoreceptor arrays in cryotomograms of intact Caulobacter crescentus cells. (nih.gov)
  • A) Caulobacter cells divide asymmetrically to yield a swarmer and a stalked cell, which are mixed in culture. (asm.org)
  • Caulobacter is an alpha-proteobacterium that always divides asymmetrically to generate two daughter cells that are morphologically distinct and have different replicative capacities. (mit.edu)
  • To study the organization of bacterial chromosomes with high resolution, we used Hi-C on Caulobacter cells (figs. S1 and S2). (sciencemag.org)
  • C. vibrioides) swarmer and stalked cells starved for carbon GSE25998: Expression data from WT, DSigT and DSigU Caulobacter crescentus (syn. (omicsdi.org)
  • We report a study by fluorescence imaging and atomic force microscopy on the growth in size and thickness of the holdfast of synchronized Caulobacter crescentus cells as they attach to a glass surface. (biomedcentral.com)
  • Expression of fliX is under cell cycle control, with transcription beginning relatively early in the cell cycle and peaking in Caulobacter predivisional cells. (umn.edu)
  • Caulobacter crescentus undergoes a series of programmed differentiation events within each cell cycle and generates two dissimilar progeny cells, a motile swarmer cell possessing a single polar flagellum and a sessile stalked cell. (beds.ac.uk)
  • Transfer and expression of Pseudomonas plasmid RP1 in Caulobacter. (microbiologyresearch.org)
  • Analysis of the dynamics of TrfA- complex formation during the Caulobacter cell cycle revealed that TrfA binds primarily during the G1 phase, however plasmid DNA synthesis occurs during the S and G2 phases of the cell cycle. (unibas.ch)
  • Yet, Caulobacter has the swarmer cell stage that results in slower population growth. (wikipedia.org)
  • Almqvist H, Jonsdottir Glaser S, Tufvegren C, Wasserstrom L, Lidén G. Characterization of the Weimberg Pathway in Caulobacter crescentus . (mdpi.com)
  • While reproducing a key result from Easter and Gober (2002) that showed Caulobacter crescentus ParA-ATP dissociated pre-bound ParB from parS ( Easter and Gober, 2002 ), we found that CTP could also modulate the nucleation of Caulobacter ParB on parS . (elifesciences.org)
  • DipM links peptidoglycan remodelling to outer membrane organization in Caulobacter. (helmholtz-hzi.de)
  • In Caulobacter crescentus , a bacterium known for studies of bacterial cell division cycle, the response regulator CpdR couples phosphorylation events with the AAA+ protease ClpXP to provide punctuated degradation of crucial substrates involved in cell cycle regulation. (umass.edu)
  • In this thesis, I use the model system Caulobacter crescentus to examine the regulation of CtrA, the essential transcription factor at the core of the Caulobacter cell cycle regulatory network. (mit.edu)
  • Modeling Asymmetric Cell Division in Caulobacter crescentus Using a Boolean Logic Approach. (ginsim.org)
  • Two examples of retraction of the Caulobacter crescentus pilus . (eurekalert.org)
  • Phylogeny by a polyphasic approach of the order Caulobacterales, proposal of Caulobacter mirabilis sp. (ox.ac.uk)
  • Perez, J. Epigenetics Theoretical Limits of Synthetic Genomes: The Cases of Artificials Caulobacter ( C. eth-2.0), Mycoplasma Mycoides (JCVI-Syn 1.0, JCVI-Syn 3.0 and JCVI_3A), E-coli and YEAST chr XII . (preprints.org)
  • The link between the City University of New York and Caulobacter crescentus dates back far before this study, however, as Brooklyn College alumnus Lucy Shapiro pioneered the study of Caulobacter crescentus as a model system. (eurekalert.org)