Cationic Amino Acid Transporter 1: A high-affinity, low capacity system y+ amino acid transporter found ubiquitously. It has specificity for the transport of ARGININE; LYSINE; and ORNITHINE. It may also act as an ecotropic leukemia retroviral receptor.Cationic Amino Acid Transporter 2: A high-affinity, low capacity system y+ amino acid transporter with strong similarity to CATIONIC AMINO ACID TRANSPORTER 1. The two isoforms of the protein, CAT-2A and CAT-2B, exist due to alternative mRNA splicing. The transporter has specificity for the transport of ARGININE; LYSINE; and ORNITHINE.Amino Acid Transport Systems, Basic: Amino acid transporter systems capable of transporting basic amino acids (AMINO ACIDS, BASIC).Amino Acid Transport Systems: Cellular proteins and protein complexes that transport amino acids across biological membranes.Arginine: An essential amino acid that is physiologically active in the L-form.Amino Acid Transport System y+LAmino Acid Transport System y+Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Arginase: A ureahydrolase that catalyzes the hydrolysis of arginine or canavanine to yield L-ornithine (ORNITHINE) and urea. Deficiency of this enzyme causes HYPERARGININEMIA. EC 3.5.3.1.Large Neutral Amino Acid-Transporter 1: A CD98 antigen light chain that when heterodimerized with CD98 antigen heavy chain (ANTIGENS, CD98 HEAVY CHAIN) forms a protein that mediates sodium-independent L-type amino acid transport.Antigens, CD98 Heavy Chain: A transmembrane glycoprotein subunit that can dimerize with a variety of light chain subunits (ANTIGENS, CD98 LIGHT CHAINS). This protein subunit serves a diverse array of functions including amino acid transport and cell fusion. Its function is altered depending which of the light chain subunits it interacts with.Amino Acid Transport Systems, Neutral: Amino acid transporter systems capable of transporting neutral amino acids (AMINO ACIDS, NEUTRAL).Antigens, CD98: A heterodimeric protein that is a cell surface antigen associated with lymphocyte activation. The initial characterization of this protein revealed one identifiable heavy chain (ANTIGENS, CD98 HEAVY CHAIN) and an indeterminate smaller light chain. It is now known that a variety of light chain subunits (ANTIGENS, CD98 LIGHT CHAINS) can dimerize with the heavy chain. Depending upon its light chain composition a diverse array of functions can be found for this protein. Functions include: type L amino acid transport, type y+L amino acid transport and regulation of cellular fusion.Antigens, CD98 Light Chains: A family of light chains that bind to the CD98 heavy chain (ANTIGENS, CD98 HEAVY CHAIN) to form a heterodimer. They convey functional specificity to the protein.Excitatory Amino Acid Transporter 1: A glial type glutamate plasma membrane transporter protein found predominately in ASTROCYTES. It is also expressed in HEART and SKELETAL MUSCLE and in the PLACENTA.Receptors, Virus: Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.Amino Acid Transport System ASC: A ubiquitous sodium-dependent neutral amino acid transporter. The preferred substrates for this transporter system include ALANINE; SERINE; and CYSTEINE.Excitatory Amino Acid Transporter 3: A neuronal and epithelial type glutamate plasma membrane transporter protein.Amino Acid Transport System A: A sodium-dependent neutral amino acid transporter that accounts for most of the sodium-dependent neutral amino acid uptake by mammalian cells. The preferred substrates for this transporter system include ALANINE; SERINE; and GLUTAMINE.Excitatory Amino Acid Transporter 2: A glutamate plasma membrane transporter protein found in ASTROCYTES and in the LIVER.Excitatory Amino Acid Transporter 4: A glutamate plasma membrane transporter protein that is primarily expressed in cerebellar PURKINJE CELLS on postsynaptic DENDRITIC SPINES.Amino Acids, Neutral: Amino acids with uncharged R groups or side chains.Lysine: An essential amino acid. It is often added to animal feed.Xenopus laevis: The commonest and widest ranging species of the clawed "frog" (Xenopus) in Africa. This species is used extensively in research. There is now a significant population in California derived from escaped laboratory animals.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acids, DiaminoCations: Positively charged atoms, radicals or groups of atoms which travel to the cathode or negative pole during electrolysis.Nitric Oxide Synthase: An NADPH-dependent enzyme that catalyzes the conversion of L-ARGININE and OXYGEN to produce CITRULLINE and NITRIC OXIDE.Glutamate Plasma Membrane Transport Proteins: A family of plasma membrane neurotransmitter transporter proteins that couple the uptake of GLUTAMATE with the import of SODIUM ions and PROTONS and the export of POTASSIUM ions. In the CENTRAL NERVOUS SYSTEM they regulate neurotransmission through synaptic reuptake of the excitatory neurotransmitter glutamate. Outside the central nervous system they function as signal mediators and regulators of glutamate metabolism.Nitric Oxide Synthase Type II: A CALCIUM-independent subtype of nitric oxide synthase that may play a role in immune function. It is an inducible enzyme whose expression is transcriptionally regulated by a variety of CYTOKINES.Amino Acid Transport System L: A sodium-independent neutral amino acid transporter system with specificity for large amino acids. One of the functions of the transporter system is to supply large neutral amino acids to the brain.Excitatory Amino Acid Transporter 5: A glutamate plasma membrane transporter protein that is primarily expressed in the RETINA.Ornithine: An amino acid produced in the urea cycle by the splitting off of urea from arginine.Amino Acid Transport System X-AG: A family of POTASSIUM and SODIUM-dependent acidic amino acid transporters that demonstrate a high affinity for GLUTAMIC ACID and ASPARTIC ACID. Several variants of this system are found in neuronal tissue.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Symporters: Membrane transporters that co-transport two or more dissimilar molecules in the same direction across a membrane. Usually the transport of one ion or molecule is against its electrochemical gradient and is "powered" by the movement of another ion or molecule with its electrochemical gradient.Nitric Oxide: A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.Leucine: An essential branched-chain amino acid important for hemoglobin formation.Membrane Glycoproteins: Glycoproteins found on the membrane or surface of cells.Oocytes: Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).Membrane Transport Proteins: Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Organic Anion Transporters, Sodium-Dependent: A subclass of ORGANIC ANION TRANSPORTERS whose transport of organic anions is driven either directly or indirectly by a gradient of sodium ions.Organic Anion Transporters: Proteins involved in the transport of organic anions. They play an important role in the elimination of a variety of endogenous substances, xenobiotics and their metabolites from the body.Kinetics: The rate dynamics in chemical or physical systems.Biological Transport, Active: The movement of materials across cell membranes and epithelial layers against an electrochemical gradient, requiring the expenditure of metabolic energy.Cystine: A covalently linked dimeric nonessential amino acid formed by the oxidation of CYSTEINE. Two molecules of cysteine are joined together by a disulfide bridge to form cystine.Sodium: A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.ATP-Binding Cassette Transporters: A family of MEMBRANE TRANSPORT PROTEINS that require ATP hydrolysis for the transport of substrates across membranes. The protein family derives its name from the ATP-binding domain found on the protein.GABA Plasma Membrane Transport Proteins: A family of plasma membrane neurotransmitter transporter proteins that regulates extracellular levels of the inhibitory neurotransmitter GAMMA-AMINOBUTYRIC ACID. They differ from GABA RECEPTORS, which signal cellular responses to GAMMA-AMINOBUTYRIC ACID. They control GABA reuptake into PRESYNAPTIC TERMINALS in the CENTRAL NERVOUS SYSTEM through high-affinity sodium-dependent transport.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Cystinuria: An inherited disorder due to defective reabsorption of CYSTINE and other BASIC AMINO ACIDS by the PROXIMAL RENAL TUBULES. This form of aminoaciduria is characterized by the abnormally high urinary levels of cystine; LYSINE; ARGININE; and ORNITHINE. Mutations involve the amino acid transport protein gene SLC3A1.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Ethylmaleimide: A sulfhydryl reagent that is widely used in experimental biochemical studies.Cysteamine: A mercaptoethylamine compound that is endogenously derived from the COENZYME A degradative pathway. The fact that cysteamine is readily transported into LYSOSOMES where it reacts with CYSTINE to form cysteine-cysteamine disulfide and CYSTEINE has led to its use in CYSTINE DEPLETING AGENTS for the treatment of CYSTINOSIS.Amino Acids, Cyclic: A class of amino acids characterized by a closed ring structure.Amino Acids, Essential: Amino acids that are not synthesized by the human body in amounts sufficient to carry out physiological functions. They are obtained from dietary foodstuffs.Cystinosis: A metabolic disease characterized by the defective transport of CYSTINE across the lysosomal membrane due to mutation of a membrane protein cystinosin. This results in cystine accumulation and crystallization in the cells causing widespread tissue damage. In the KIDNEY, nephropathic cystinosis is a common cause of RENAL FANCONI SYNDROME.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Amino Acid Substitution: The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.Renal Aminoacidurias: A group of inherited kidney disorders characterized by the abnormally elevated levels of AMINO ACIDS in URINE. Genetic mutations of transport proteins result in the defective reabsorption of free amino acids at the PROXIMAL RENAL TUBULES. Renal aminoaciduria are classified by the specific amino acid or acids involved.Glutamic Acid: A non-essential amino acid naturally occurring in the L-form. Glutamic acid is the most common excitatory neurotransmitter in the CENTRAL NERVOUS SYSTEM.Glutamine: A non-essential amino acid present abundantly throughout the body and is involved in many metabolic processes. It is synthesized from GLUTAMIC ACID and AMMONIA. It is the principal carrier of NITROGEN in the body and is an important energy source for many cells.Putrescine: A toxic diamine formed by putrefaction from the decarboxylation of arginine and ornithine.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Monocarboxylic Acid Transporters: A family of proteins involved in the transport of monocarboxylic acids such as LACTIC ACID and PYRUVIC ACID across cellular membranes.Kidney: Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.Hartnup Disease: An autosomal recessive disorder due to defective absorption of NEUTRAL AMINO ACIDS by both the intestine and the PROXIMAL RENAL TUBULES. The abnormal urinary loss of TRYPTOPHAN, a precursor of NIACIN, leads to a NICOTINAMIDE deficiency, PELLAGRA-like light-sensitive rash, CEREBELLAR ATAXIA, emotional instability, and aminoaciduria. Mutations involve the neurotransmitter transporter gene SLC6A19.Amino Acids, Aromatic: Amino acids containing an aromatic side chain.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Vesicular Inhibitory Amino Acid Transport Proteins: A family of vesicular neurotransmitter transporter proteins that sequester the inhibitory neurotransmitters GLYCINE; GAMMA-AMINOBUTYRIC ACID; and possibly GAMMA-HYDROXYBUTYRATE into SECRETORY VESICLES.Amino Acid Metabolism, Inborn Errors: Disorders affecting amino acid metabolism. The majority of these disorders are inherited and present in the neonatal period with metabolic disturbances (e.g., ACIDOSIS) and neurologic manifestations. They are present at birth, although they may not become symptomatic until later in life.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Nucleotide Transport Proteins: Proteins involved in the transport of NUCLEOTIDES across cellular membranes.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)PolyaminesBiogenic Polyamines: Biogenic amines having more than one amine group. These are long-chain aliphatic compounds that contain multiple amino and/or imino groups. Because of the linear arrangement of positive charge on these molecules, polyamines bind electrostatically to ribosomes, DNA, and RNA.Amino Acid Transport Disorders, Inborn: Disorders characterized by defective transport of amino acids across cell membranes. These include deficits in transport across brush-border epithelial cell membranes of the small intestine (MICROVILLI) and KIDNEY TUBULES; transport across the basolateral membrane; and transport across the membranes of intracellular organelles. (From Nippon Rinsho 1992 Jul;50(7):1587-92)beta-Alanine: An amino acid formed in vivo by the degradation of dihydrouracil and carnosine. Since neuronal uptake and neuronal receptor sensitivity to beta-alanine have been demonstrated, the compound may be a false transmitter replacing GAMMA-AMINOBUTYRIC ACID. A rare genetic disorder, hyper-beta-alaninemia, has been reported.Excitatory Amino Acids: Endogenous amino acids released by neurons as excitatory neurotransmitters. Glutamic acid is the most common excitatory neurotransmitter in the brain. Aspartic acid has been regarded as an excitatory transmitter for many years, but the extent of its role as a transmitter is unclear.Aspartic Acid: One of the non-essential amino acids commonly occurring in the L-form. It is found in animals and plants, especially in sugar cane and sugar beets. It may be a neurotransmitter.Leukemia Virus, Murine: Species of GAMMARETROVIRUS, containing many well-defined strains, producing leukemia in mice. Disease is commonly induced by injecting filtrates of propagable tumors into newborn mice.Alanine: A non-essential amino acid that occurs in high levels in its free state in plasma. It is produced from pyruvate by transamination. It is involved in sugar and acid metabolism, increases IMMUNITY, and provides energy for muscle tissue, BRAIN, and the CENTRAL NERVOUS SYSTEM.Mutagenesis, Site-Directed: Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.Xenopus: An aquatic genus of the family, Pipidae, occurring in Africa and distinguished by having black horny claws on three inner hind toes.Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Glycine: A non-essential amino acid. It is found primarily in gelatin and silk fibroin and used therapeutically as a nutrient. It is also a fast inhibitory neurotransmitter.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Structure-Activity Relationship: The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Recombinant Proteins: Proteins prepared by recombinant DNA technology.

Two-way arginine transport in human endothelial cells: TNF-alpha stimulation is restricted to system y(+). (1/75)

Human umbilical vein endothelial cells transport arginine through two Na(+)-independent systems. System y(+)L is insensitive to N-ethylmaleimide (NEM), inhibited by L-leucine in the presence of Na(+), and referable to the expression of SLC7A6/y(+)LAT2, SLC7A7/y(+)LAT1, and SLC3A2/4F2hc. System y(+) is referable to the expression of SLC7A1/CAT1 and SLC7A2/CAT2B. Tumor necrosis factor-alpha (TNF-alpha) and bacterial lipopolysaccharide induce a transient stimulation of arginine influx and efflux through system y(+). Increased expression of SLC7A2/CAT2B is detectable from 3 h of treatment, while SLC7A1 expression is inhibited at later times of incubation. System y(+)L activity and expression remain unaltered. Nitric oxide synthase type 2 mRNA is not detected in the absence or presence of TNF-alpha, while the latter condition lowers nitric oxide synthase type 3 expression at the mRNA and the protein level. Nitrite accumulation is comparable in cytokine-treated and control cells up to 48 h of treatment. It is concluded that modulation of endothelial arginine transport by TNF-alpha or lipopolysaccharide occurs exclusively through changes in CAT2B and CAT1 expression and is dissociated from stimulation of nitric oxide production.  (+info)

Fv1-like restriction of N-tropic replication-competent murine leukaemia viruses in mCAT-1-expressing human cells. (2/75)

To study the replication of murine leukaemia viruses in human cells we have used full-length as well as EGFP-tagged ecotropic viruses in combination with mCAT-1-expressing human cells. We present results showing that N-tropic murine leukaemia viruses are restricted in both infection and replication in such cells while B-tropic viruses, modified at capsid position 110, escape restriction. These results support a recently reported Fv1-like restriction in mammalian cells. We extend the analysis of Fv1-like restriction by demonstrating that NB-tropic viruses also escape restriction and human mCAT-1-expressing cells are thus similar to murine Fv1(b) cells with respect to infection though the ecotropic receptor pathway.  (+info)

Garlic attenuates nitric oxide production in rat cardiac myocytes through inhibition of inducible nitric oxide synthase and the arginine transporter CAT-2 (cationic amino acid transporter-2). (3/75)

It is now accepted that allicin, the main biologically active compound in garlic, exhibits antioxidant activity. The present study was designed to test the hypothesis that the antioxidant activity of garlic can be partially attributed to the inhibition of nitric oxide (NO) production by cytokine-induced NO synthase (iNOS). Cardiac myocytes cultured from neonatal Wistar rats were stimulated by lipopolysaccharide (LPS) and incubated for 24 h with various concentrations of allicin. This resulted in marked inhibition of nitrite production. Interestingly, a low concentration of allicin (10 microM) was significantly more potent in abrogating the effect of LPS on nitrite production than a higher concentration (40 microM). Allicin decreased steady-state iNOS mRNA levels, and this effect was maximal when a lower concentration was used (10 microM compared with 40 microM). In order to explore additional effects of allicin on NO generation that might counteract the effect on iNOS, we assessed the effects of higher allicin concentrations on arginine transport. Allicin inhibited the uptake of 1 mM extracellular arginine in a concentration-dependent manner. The expression of the two arginine transporters that are expressed in cardiac myocytes [CAT-1 (cationic amino acid transporter-1) and CAT-2] was studied using reverse transcription-PCR. A concentration of 200 microM allicin abolished the expression of CAT-2 mRNA, 100 microM significantly attenuated it, whereas 50 microM had no effect. Allicin had no effect on steady-state CAT-1 mRNA levels. Our results suggest that allicin inhibits iNOS activity through two different mechanisms: at lower concentrations it decreases iNOS mRNA levels, whereas at higher concentrations it inhibits arginine transport through down-regulation of CAT-2 mRNA.  (+info)

Intrauterine growth retardation is associated with reduced activity and expression of the cationic amino acid transport systems y+/hCAT-1 and y+/hCAT-2B and lower activity of nitric oxide synthase in human umbilical vein endothelial cells. (4/75)

Intrauterine growth retardation (IUGR) is associated with vascular complications leading to hypoxia and abnormal fetal development. The effect of IUGR on L-arginine transport and nitric oxide (NO) synthesis was investigated in cultures of human umbilical vein endothelial cells (HUVECs). IUGR was associated with membrane depolarization and reduced L-arginine transport (V(max)= 5.8+/-0.2 versus 3.3+/-0.1 pmol/microg protein per minute), with no significant changes in transport affinity (K(m)=159+/-15 versus 137+/-14 micromol/L). L-Arginine transport was trans-stimulated (8- to 9-fold) in cells from normal and IUGR pregnancies. IUGR was associated with reduced production of L-[3H]citrulline from L-[3H] arginine, lower nitrite and intracellular L-arginine, L-citrulline, and cGMP. IUGR decreased hCAT-1 and hCAT-2B mRNA, and increased eNOS mRNA and protein levels. IUGR-associated inhibition of L-arginine transport and NO synthesis, and membrane depolarization were reversed by the NO donor S-nitroso-N-acetyl-L,D-penicillamine. In summary, endothelium from fetuses with IUGR exhibit altered L-arginine transport and NO synthesis (L-arginine/NO pathway), reduced expression and activity of hCAT-1 and hCAT-2B and reduced eNOS activity. Alterations in L-arginine/NO pathway could be critical for the physiological processes involved in the etiology of IUGR in human pregnancies.  (+info)

Nutritional control of mRNA stability is mediated by a conserved AU-rich element that binds the cytoplasmic shuttling protein HuR. (5/75)

The cationic amino acid transporter, Cat-1, is a high affinity transporter of the essential amino acids, arginine and lysine. Expression of the cat-1 gene increases during nutritional stress as part of the adaptive response to starvation. Amino acid limitation induces coordinate increases in stability and translation of the cat-1 mRNA, at a time when global protein synthesis decreases. It is shown here that increased cat-1 mRNA stability requires an 11 nucleotide AU-rich element within the distal 217 bases of the 3'-untranslated region. When this 217-nucleotide nutrient sensor AU-rich element (NS-ARE) is present in a chimeric mRNA it confers mRNA stabilization during amino acid starvation. HuR is a member of the ELAV family of RNA-binding proteins that has been implicated in regulating the stability of ARE-containing mRNAs. We show here that the cytoplasmic concentration of HuR increases during amino acid starvation, at a time when total cellular HuR levels decrease. In addition, RNA gel shift experiments in vitro demonstrated that HuR binds to the NS-ARE and binding was dependent on the 11 residue AU-rich element. Moreover, HuR binding to the NS-ARE in extracts from amino acid-starved cells increased in parallel with the accumulation of cytoplasmic HuR. It is proposed that an adaptive response of cells to nutritional stress results in increased mRNA stability mediated by HuR binding to the NS-ARE.  (+info)

L-Arginine transport is augmented through up-regulation of tubular CAT-2 mRNA in ischemic acute renal failure in rats. (6/75)

BACKGROUND: Ischemic acute renal failure (iARF) is associated with increased nitric oxide (NO) production during the reperfusion period, as endothelial nitric oxide synthase (eNOS) is maximally activated, and renal tubular inducible NOS (iNOS) is stimulated. Increased NO production leads to augmented tubular injury, probably through the formation of peroxynitrite. l-Arginine (l-Arg), the only precursor for NO, is transported into cells by cationic amino acid transporters, CAT-1 and CAT-2. We hypothesized that the increased NO production observed in iARF may result from increased l-Arg uptake, which would be reflected in the augmented expression of l-Arg transporter(s). METHODS: Ischemic acute renal failure was induced in rats by right nephrectomy + left renal artery clamping for 60 minutes. l-Arg uptake was examined in freshly harvested glomeruli and tubuli from control, sham operated, and animals subjected to 15, 30, and 60 minutes, and 24 hours of reperfusion, following 60 minutes of ischemia. Using RT-PCR, renal tissues were examined further for the expression of iNOS, CAT-1, CAT-2, arginase I and arginase II. RESULTS: Tubular expression of iNOS mRNA was initiated by ischemia, continued to increase after 60 minutes of reperfusion, and decreased after 24 hours. l-Arg transport into glomeruli was similar in all experimental groups. l-Arg uptake into tubuli was markedly augmented following the 60-minute reperfusion, while it moderately increased after 24 hours of reperfusion. This was accompanied by a parallel, preferential increase in tubular CAT-2 mRNA expression at 60 minutes of reperfusion. CAT-1 mRNA expression was unchanged, as detected by RT-PCR. In addition, the expression of arginase II and arginase I mRNA was attenuated by 30 minutes and one hour of reperfusion, and returned to baseline values after 24 hours of reperfusion. CONCLUSIONS: Ischemic ARF is associated with augmented tubular CAT-2 mRNA expression, which leads to enhanced l-Arg transport and increased NO production. This may contribute to the renal injury exhibited in iARF.  (+info)

System y+ localizes to different membrane subdomains in the basolateral plasma membrane of epithelial cells. (7/75)

We report here that the system y+ cationic amino acid transporter ATRC1 localized to clusters within the basolateral membrane of polarized Madin-Darby canine kidney and human embryonic kidney (HEK) cells, suggesting that the transporters are restricted to discrete membrane microdomains in epithelial cells. Based on solubility in nonionic detergents, two populations of ATRC1 molecules existed: approximately half of the total ATRC1 in HEK cells associated with the actin membrane cytoskeleton, whereas another one-fourth resided in detergent-resistant membranes (DRM). In agreement with these findings, cytochalasin D reduced the amount of ATRC1 associated with the actin membrane cytoskeleton. Although some ATRC1 clusters in HEK cells colocalized with caveolin, the majority of ATRC1 did not colocalize with this marker protein for a type of DRM called caveolae. This distribution of ATRC1 is somewhat different from that reported for pulmonary artery endothelial cells in which transporters cluster predominantly in caveolae, suggesting that differences in the proportion of ATRC1 in specific membrane microdomains correlate with differences in the physiological role of the transporter in polarized kidney epithelial vs. vascular endothelial cells.  (+info)

Regulation of the S100B gene by alpha 1-adrenergic stimulation in cardiac myocytes. (8/75)

We previously reported that S100B, a 20-kDa Ca(2+)-binding homodimer, inhibited the postinfarct myocardial hypertrophic response mediated by alpha(1)-adrenergic stimulation through the protein kinase C (PKC) signaling pathway. In the present study, we examined whether the same pathway induced the S100B gene, supporting the hypothesis that S100B is a feedback negative regulator of this pathway. We transfected cultured neonatal rat cardiac myocytes with a luciferase reporter gene driven by the maximal human S100B promoter and progressively shorter segments of this promoter sequentially deleted from the 5' end. We identified a basic promoter essential for transcription spanning 162 bp upstream of the transcription initiation site and positive (at -782/-162 and -6,689/-4,463) and negative (at -4,463/-782) myocyte-selective regulatory elements. We showed that the basic and maximal S100B promoters were activated specifically by alpha(1)-adrenergic agonists through the alpha(1A)-adrenergic receptor, but not by any other trophic hormonal stimuli. The activation of the S100B promoter was mediated through the PKC signaling pathway. Transcription enhancer factor-1 (TEF-1) and related to TEF-1 (RTEF-1) influenced transcription from the maximal, but not the basic, promoter implicating active MCAT elements upstream from the basic promoter. Acting in opposing fashions, TEF-1 transrepressed the S100B promoter and RTEF-1 transactivated the promoter. Our results suggest that alpha(1)-adrenergic stimulation induces the S100B gene after myocardial infarction through the PKC signaling pathway and that this induction is modulated by TEF-1 and RTEF-1.  (+info)

Hoshide R, Ikeda Y, Karashima S, Matsuura T, Komaki S, Kishino T, Niikawa N, Endo F, Matsuda I (Mar 1997). "Molecular cloning, tissue distribution, and chromosomal localization of human cationic amino acid transporter 2 (HCAT2)". Genomics. 38 (2): 174-8. doi:10.1006/geno.1996.0613. PMID 8954799 ...
TY - JOUR. T1 - Heme oxygenase 1, nuclear factor E2-related factor 2, and nuclear factor κb are involved in hemin inhibition of type 2 cationic amino acid transporter expression and L-arginine transport in stimulated macrophages. AU - Tsai, Pei Shan. AU - Chen, Chien Chuan. AU - Tsai, Pei-Shan. AU - Yang, Lin Cheng. AU - Huang, Wan Yu. AU - Huang, Chun Jen. PY - 2006/12. Y1 - 2006/12. N2 - BACKGROUND: l-Arginine transport mediated by type 2 cationic amino acid transporter (CAT-2) is one crucial mechanism that regulates nitric oxide production mediated by inducible nitric oxide synthase. Heme oxygenase (HO)-1 induction has been reported to significantly attenuate inducible nitric oxide synthase expression and nitric oxide production. The authors sought to explore the effects of HO-1 induction on CAT-2 expression and l-arginine transport. The effects of HO-1 induction on nuclear factor E2-related factor 2 (Nrf2) and nuclear factor κB (NF-κB) were also investigated. METHODS: Murine macrophages ...
TY - JOUR. T1 - HO-1 Overexpression Attenuates Endotoxin Effects on CAT-2 Isozymes Expression. AU - Huang, Te Yang. AU - Tsai, Pei Shan. AU - Huang, Chun Jen. PY - 2008/8. Y1 - 2008/8. N2 - Background: l-arginine transport mediated by type-2 cationic amino acid transporter (CAT-2) isozymes is one crucial mechanism that regulates nitric oxide (NO) production via inducible nitric oxide synthase (iNOS). We sought to investigate the effects of heme oxygenase-1 (HO-1) overexpression on CAT-2 isozymes, e.g., CAT-2, CAT-2A, and CAT-2B. Materials and Methods: Adult male Sprague Dawley rats were allocated to receive lipopolysaccharide (LPS), normal saline, hemin (a HO-1 inducer), tin protoporphyrin (SnPP, a HO-1 inhibitor), LPS plus hemin, or LPS plus hemin plus SnPP. After maintaining for 6 h, rats were sacrificed and the expression and activity of individual enzyme was evaluated. Results: LPS increased HO activity, HO-1 concentration, NO production, l-arginine transport, and concentrations of iNOS, ...
Infection of rodent cells by ecotropic type C retroviruses requires the expression of a cationic amino acid transporter composed of multiple membrane-spanning domains. By exchanging portions of cDNAs encoding the permissive mouse and nonpermissive human transporters and examining their abilities to specify virus infection upon expression in human 293 cells, we have identified the amino acid residues in the extracellular loop connecting the fifth and sixth membrane-spanning segments of the mouse transporter that are required for both envelope gp70 binding and infection. These findings strongly suggest that the role of the mouse transporter in determining infection is to provide an envelope-binding site. This role is analogous to those of host membrane proteins composed of a single membrane-spanning domain that serve as binding proteins or receptors for other enveloped viruses such as human immunodeficiency virus, Epstein-Barr virus, and murine and human coronaviruses. ...
Slc7a3 (untagged) - Mouse solute carrier family 7 (cationic amino acid transporter, y+ system), member 3 (Slc7a3), (10ug), 10 µg.
MDSCs are one of the dominant immunosuppressive populations that are present in the tumor microenvironment, impairing T cell function and promoting tumor progression (30). Therefore, MDSCs present a major obstacle for the success of cancer immunotherapy. As a result, blocking MDSC function has been an attractive endeavor to complement cancer therapies. Indeed, several studies demonstrated that depletion of MDSCs or inhibiting MDSC function impaired cancer progression (31, 32). Although these studies are very promising, more specific strategies to block MDSC suppressive function are needed.. MDSCs mediate their inhibitory effects on T cells through diverse mechanisms (33). One mechanism is the metabolism of amino acids by MDSCs (34). For example, MDSCs express enzymes that metabolize l-Arg, l-tryptophan, and cysteine, leading to their consumption from the microenvironment. Depletion of these amino acids results in T cell dysfunction (35-37). In addition, MDSCs can metabolize l-Arg and ...
Homo sapiens solute carrier family 7 (cationic amino acid transporter, y+ system), member 5 (SLC7A5), mRNA. (H00008140-R01) - Products - Abnova
pep:novel chromosome:VEGA66:8:40898438:40917726:1 gene:OTTMUSG00000061152 transcript:OTTMUST00000078365 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Slc7a2 description:solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 ...
pep:known chromosome:VEGA66:16:17572018:17576721:-1 gene:OTTMUSG00000035925 transcript:OTTMUST00000092151 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Slc7a4 description:solute carrier family 7 (cationic amino acid transporter, y+ system), member 4 ...
Thank you for your interest in spreading the word about Biochemical Journal.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
The present invention provides a reagent for introducing a protein or gene into a cell. The reagent of the present invention is, for example, a reagent for introducing a protein or gene into a cell, which comprises a composition comprising a cationic amino acid type lipid represented by the following formula (I)-1: (wherein in formula (I)-1: L is a single bond, -CONH-, or -S-S-; M1 is -(CH2)k- or -(CH2CH2O)k- (wherein k is an integer between 0 and 14); and m1 and m2 are each independently an integer between 11 and 21 (in this regard, when providing a reagent for introducing a gene into a cell, the case where both m1 and m2 are 15 is excluded)).
More than 50 distinct amino acid transporter genes have been identified in the genome of Arabidopsis, indicating that transport of amino acids across membranes is a highly complex feature in plants. Based on sequence similarity, these transporters can be divided into two major superfamilies: the amino acid transporter family and the amino acid polyamine choline transporter family. Currently, mainly transporters of the amino acid transporter family have been characterized. Here, a molecular and functional characterization of amino acid polyamine choline transporters is presented, namely the cationic amino acid transporter (CAT) subfamily. CAT5 functions as a high-affinity, basic amino acid transporter at the plasma membrane. Uptake of toxic amino acid analogs implies that neutral or acidic amino acids are preferentially transported by CAT3, CAT6, and CAT8. The expression profiles suggest that CAT5 may function in reuptake of leaking amino acids at the leaf margin, while CAT8 is expressed in young ...
Dropbox is a free service that lets you bring your photos, docs, and videos anywhere and share them easily. Never email yourself a file again!
Amino acids represent a strong signal that positively regulates mTORC1 (reviewed by Guertin and Sabatini, 2007). It was recently shown that leucine, an essential amino acid required for mTORC1 activation, is transported into cells in a glutamine-dependent fashion (Nicklin et al., 2009). Glutamine, which is imported into cells through SLC1A5 [solute carrier family 1 (neutral amino acid transporter) member 5], is exchanged to import leucine via a heterodimeric system composed of SLC7A5 [antiport solute carrier family 7 (cationic amino acid transporter, y+ system, member 5] and SLC3A2 [solute carrier family 3 (activators of dibasic and neutral amino acid transport) member 2]. The mechanism by which intracellular amino acids then signal to mTORC1 remained obscure for many years. The activation of mTORC1 by amino acids is known to be independent of TSC1/2, because the mTORC1 pathway remains sensitive to amino acid deprivation in cells that lack TSC1 or TSC2 (Nobukuni et al., 2005). Some studies have ...
Shop INTELLINET 50 CAT-5e RJ-45 UTP Network Patch Cable, Gray at Staples. Choose from our wide selection of INTELLINET 50 CAT-5e RJ-45 UTP Network Patch Cable, Gray and get fast & free shipping on select orders.
Arginase, Arginine Uptake and their Role in Leishmania (L.) amazonensis Physiology. Lucile Floeter-Winter, Universidade de São Paulo, Brazil ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
This website is run by the accessibility program of the "Accessible with a Click" company and is run via a designated accessibility server. The program allows the website to follow the guidelines for internet content accessibility WCAG 2.0 to level AA. The program is subject to the conditions of use of the manufacturer. Warrantee of use applies to the website owners and/or their representative, including the content displayed in the website, as subject to the conditions of use ...
This study has focused on the in vitro and in vivo characterization of a second-generation CD22-targeted immunotoxin, CAT-8015. The results from the in vitro cytotoxicity assays showed that CAT-8015 is highly effective in killing B cells from a variety of tumor cell lines. In addition, CAT-8015 was found to be up to approximately 12-fold more potent than CAT-3888 in cell survival-based assays. In vitro binding studies with human or monkey PBMCs showed that when used at an equal concentration, a higher relative mean fluorescence intensity signal was observed for CAT-8015 compared with CAT-3888, suggesting that the increase in the affinity of the targeting scFv for CD22 results in an increase in receptor occupancy. The pharmacokinetic profiles of CAT-8015 were evaluated in single dose studies in mice, rats, and monkeys. These studies estimated the plasma clearance to be 0.04, 0.23, and approximately 2.0 to 4.0 mL/min in mice, rats, and monkeys, respectively. Although a single administration of ...
The Original MCAT Question of the Day! Your source for MCAT 2015 practice questions. Free MCAT test prep since 2008. Thousands of free questions, services, and an online MCAT course.
Study Flashcards On Med-Surg Nursing - 26 at Cram.com. Quickly memorize the terms, phrases and much more. Cram.com makes it easy to get the grade you want!
Sodium-dependent neutral amino acid transporter-2 (SNAT2), the ubiquitous member of SLC38 family, accounts for the activity of transport system A for neutral amino acids in most mammalian tissues. As the transport process performed by SNAT2 is highly
Arginine uptake and degradation systems are common throughout bacteria and archaea. The genome of human pathogen Chlamydia pneumoniae encodes three proteins now called AaxA, AaxB, and AaxC which function together to take ...
Find SLC3: Heavy Subunits of the Heteromeric Amino Acid Transporter Family research area related information and SLC3: Heavy Subunits of the Heteromeric Amino Acid Transporter Family research products from R&D Systems. Learn more.
And in the unfortunate case that you do believe that you are running short of choline, because you dont eat all the good choline containing foods out of ethical or whatever other reasons, and thus insist on supplementing, I suggest you yourself a 500g container of choline bitartrate powder (dont let that become wet, though! It will stink like rotten fish ;-). Those 500g of choline bitartrate (40% choline, 60% tartate) will cost you about as much as 60x300mg caps of the overpriced GPC and has been "scientifically proven" (not in supplement company terms, but in SuppVersity terms) to safely increase circulating and brain choline levels and its metabolites after oral ingestion, as well (Stoll. 1996; Babb. 2004). And lets be honest, even if the effects on growth hormone were GPC specific - even on the boards, people have meanwhile realized none of those arginine + lysine GH boosters does make a difference and not because they would not produce transient increases in GH, but simply because those ...
l-Arginine did not produce the anticipated reversal of insulin resistance produced byl-NAME, but, rather,l-arginine by itself caused insulin resistance (48.8 ± 8.2%) (Fig. 5).l-NAME not only blocks NOS but also blocks arginine uptake across the hepatocyte plasma membrane (8).l-Arginine is metabolized by NOS to NO and by arginase to urea andl-ornithine (6). Because the liver has a very high arginase activity, it is possible that mostl-arginine administered is converted to l-ornithine by the liver, although l-arginine can reverse the vascular effects ofl-NAME in the liver (12).l-Arginine also causes release of growth hormone (7, 14) and glucagon; both hormones reduce insulin sensitivity. This may explain why we could not reverse insulin resistance caused by l-NAME withl-arginine and whyl-arginine caused insulin resistance.. Reduction in blood flow to the nerves in diabetes leads to neuropathy (3, 4, 9, 17, 25) and has been suggested to result from a decrease in NO production in the vasculature ...
Low prices on Arginine! Improve heart health, lower blood pressure and speed up wound healing*. L-arginine is an amino acid shown to have many benefits on health. Studies have shown significant improvement of symptoms as well as objective measurements of heart function. L-arginine improves the bioavailability of nitric oxide (NO), a naturally occurring substance in the body.
In this study we report that 8-iso-PGF2α, a major and stable product of lipid peroxidation, was potent in causing cell death of cultured brain microvascular endothelial cells as well as of ex vivo preparation of microvessels but not of human umbilical vein endothelium, smooth muscle, or astroglia; another related isoprostane, 8-iso-PGE2, did not trigger endothelial cell death. 8-Iso-PGF2α-induced cytotoxicity was TXA2 dependent and mostly of oncotic nature. Associated with these effects, intraventricular injection of 8-iso-PGF2α caused cystlike formations in the periventricular area.. The nature of cell death induced by 8-iso-PGF2α seems to be primarily oncotic rather than apoptotic necrosis (Figure 2). This inference is supported by the data that 8-iso-PGF2α caused ,8% of cell DNA fragmentation, a time-dependent increase in PI incorporation and LDH release, indicative of membrane disruption (Figure 2), and its effects, as well as those of its mediator TXA2, were unaltered by caspase ...
Outer membrane porin D is a protein family containing bacterial outer membrane porins which are involved in transport of cationic amino acids, peptides, antibiotics and other compounds. It was also described as having some serine protease activity. However many of these proteins are not peptidases and are classified as non-peptidase homologues as they either have been found experimentally to be without peptidase activity, or lack amino acid residues that are believed to be essential for the catalytic activity of peptidases in the S43 family. Yoshihara E, Yoneyama H, Ono T, Nakae T (June 1998). "Identification of the catalytic triad of the protein D2 protease in Pseudomonas aeruginosa". Biochem. Biophys. Res. Commun. 247 (1): 142-5. doi:10.1006/bbrc.1998.8745. PMID 9636669. This article incorporates text from the public domain Pfam and InterPro ...
Buy L-ARGININE 1000mg - 50 tabs Online.L-Arginine is an amino acid that forms part of the proteins. It is of vital importance in its isolated form as it fulfils numerous functions. Due to the role that L-Arginine plays as a vasodila
If youre preparing for the MCAT, use our flexible, comprehensive tutoring course to help you ace the exam. You can take the entire course or just...
24 yrs old Male asked about Pawan soni kelwaraplzsave, 1 doctor answered this and 24 people found it useful. Get your query answered 24*7 only on | Practo Consult
30 yrs old Male asked about Not getting conceive, 1 doctor answered this and 114 people found it useful. Get your query answered 24*7 only on | Practo Consult
Are L-Arginine benefits legit? Well, the thing is, L-Arginine is full of scientifically proven benefits, the key is, will help what ails you?
L-Arginine 500 mg Caps Each capsule provides 500 mg of L-Arginine (from L-arginine hydrochloride, L-arginine). As an Amino Acid, L-Arginine acts as one of the building blocks for protein.
... cationic amino acid transporter". Kaira K, Oriuchi N, Imai H, et al. (2009). "Prognostic significance of L-type amino acid ... 2009). "L-type amino acid transporter 1 (LAT1) is frequently expressed in thymic carcinomas but is absent in thymomas". J Surg ... 2009). "L-type amino-acid transporter 1 as a novel biomarker for high-grade malignancy in prostate cancer". Pathol. Int. 59 (1 ... 2006). "L-type amino acid transporter 1 as a potential molecular target in human astrocytic tumors". Int. J. Cancer. 119 (3): ...
Currents of Opposite Polarity Evoked by Neutral and Cationic Amino Acids in Neutral and Basic Amino Acid Transporter cRNA- ... amino acid transport on substrate affinity of the heteromeric b(0,+) amino acid transporter". J. Biol. Chem. 275 (19): 14331- ... "Effects of truncation of the COOH-terminal region of a Na+-independent neutral and basic amino acid transporter on amino acid ... dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1". Pras E ...
High affinity cationic amino acid transporter 1 is a protein that in humans is encoded by the SLC7A1 gene. Solute carrier ... Hammermann R, Brunn G, Racké K (2002). "Analysis of the genomic organization of the human cationic amino acid transporters CAT- ... Schnorr O, Suschek CV, Kolb-Bachofen V (June 2003). "The importance of cationic amino acid transporter expression in human skin ... "Entrez Gene: SLC7A1 solute carrier family 7 (cationic amino acid transporter, y+ system), member 1". Yoshimoto T, Yoshimoto E, ...
Low affinity cationic amino acid transporter 2 is a protein that in humans is encoded by the SLC7A2 gene. SLC7A2 protein, human ... 1997). "Human cationic amino acid transporters hCAT-1, hCAT-2A, and hCAT-2B: three related carriers with distinct transport ... Hammermann R, Brunn G, Racké K (2002). "Analysis of the genomic organization of the human cationic amino acid transporters CAT- ... Schnorr O, Suschek CV, Kolb-Bachofen V (2003). "The importance of cationic amino acid transporter expression in human skin". J ...
Cationic amino acid transporter 3 is a protein that in humans is encoded by the SLC7A3 gene. SLC7A3 is a member of the system ... Ito K, Groudine M (1997). "A new member of the cationic amino acid transporter family is preferentially expressed in adult ... 1999). "Neuron-specific expression of cationic amino acid transporter 3 in the adult rat brain". Brain Res. 838 (1-2): 158-65. ... 2006). "Activation of classical protein kinase C reduces the expression of human cationic amino acid transporter 3 (hCAT-3) in ...
"Entrez Gene: SLC7A8 solute carrier family 7 (cationic amino acid transporter, y+ system), member 8". Maruyama K, Sugano S (1994 ... an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids". J Biol Chem. 274 ... Large neutral amino acids transporter small subunit 2 is a protein that in humans is encoded by the SLC7A8 gene. Heterodimeric ... 2000). "LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine". J. Biol. Chem. 274 (49 ...
"Entrez Gene: SLC7A7 solute carrier family 7 (cationic amino acid transporter, y+ system), member 7". Pfeiffer, R; Rossier G; ... 1999). "Amino acid transport of y+L-type by heterodimers of 4F2hc/CD98 and members of the glycoprotein-associated amino acid ... Y+L amino acid transporter 1 is a protein that in humans is encoded by the SLC7A7 gene. SLC7A7 has been shown to interact with ... "Amino acid transport of y+L-type by heterodimers of 4F2hc/CD98 and members of the glycoprotein-associated amino acid ...
Solute carrier family 7 member 6 also known as cationic amino acid transporter, y+ system or Y+L amino acid transporter 2 is a ... cationic amino acid transporter". Pfeiffer R, Rossier G, Spindler B, Meier C, Kühn L, Verrey F (January 1999). "Amino acid ... Bröer A, Wagner CA, Lang F, Bröer S (August 2000). "The heterodimeric amino acid transporter 4F2hc/y+LAT2 mediates arginine ... Heterodimeric amino acid transporter GRCh38: Ensembl release 89: ENSG00000103064 - Ensembl, May 2017 GRCm38: Ensembl release 89 ...
Cationic amino acid transporter 4 is a protein that in humans is encoded by the SLC7A4 gene. SLC7A4 protein, human at the US ... Hammermann R, Brunn G, Racke K (Oct 2001). "Analysis of the genomic organization of the human cationic amino acid transporters ... "Entrez Gene: SLC7A4 solute carrier family 7 (cationic amino acid transporter, y+ system), member 4". Muñoz L, Lasa A, ... "The gene encoding a cationic amino acid transporter (SLC7A4) maps to the region deleted in the velocardiofacial syndrome". ...
... is processed from hcr mRNA and may downregulate the high affinity cationic amino acid transporter CAT-1". RNA Biol. 1 (2): 106- ... Coiled-coil alpha-helical rod protein 1, also known as CCHCR1, is a protein which in humans is encoded by the CCHCR1 gene. The ... It is also known as Coiled-Coil Alphahelical Rod Protein 1, C6orf18, Putative Gene 8 Protein, SBP, HCR (A-Helix Coiled-Coil Rod ... CCHCR1 coiled-coil alpha-helical rod protein 1". Asumalahti K, Laitinen T, Itkonen-Vatjus R, Lokki ML, Suomela S, Snellman E, ...
... is processed from hcr mRNA and mat downregulate the high affinity cationic amino acid transporter CAT-1". RNA Biology. 1 (2): ... miR-122 is highly expressed in the liver, where it has been implicated as a regulator of fatty-acid metabolism in mouse studies ... Fabani MM, Gait MJ (2008). "miR-122 targeting with LNA/2'-O-methyl oligonucleotide mixmers, peptide nucleic acids (PNA), and ... This change is noted before increased amino-transferase activity, making it a early indicator of liver disease and ...
"Entrez Gene: SLC7A11 solute carrier family 7, (cationic amino acid transporter, y+ system) member 11". Lutgen V, Resch J, ... This amino acid is a rate limiting substrate used in the SLC7A11 cystine/glutamate transporter and is usually imported into the ... An example is amino acid deprivation, which triggers up regulation of the transporter. A key regulator is extracellular ... The antiporter is a heterodimeric amino acid transporter. The structure of this protein includes a specific light chain, xCT, ...
... heavy subunits of heteromeric amino acid transporters (6) Bacterial Leucine Transporter (LeuT) (7) cationic amino acid ... Solute carrier family Amino acid transport Amino acid transport, acidic Amino acid transport, basic Amino acid transport ... proton-coupled amino acid transporter (38) System A & N, sodium-coupled neutral amino acid transporter Vesicular inhibitory ... An amino acid transporter is a membrane transport protein that transports amino acids. They are mainly of the solute carrier ...
This gene is predicted to encode a glycosylated, cationic amino acid transporter protein with 14 transmembrane domains. This ... and primary endothelial cells and its protein is predicted to mediate lysosomal uptake of cationic amino acids. Mutations in ... "Docking motif-guided mapping of the interactome of protein phosphatase-1". Chemistry & Biology. 16 (4): 365-71. doi:10.1016/j. ...
... dependent neutral and cationic amino acid transporter B(0+)". J Biol Chem. 274 (34): 23740-5. doi:10.1074/jbc.274.34.23740. ... dependent neurotransmitter transporter family and transports both neutral and cationic amino acids in an Na+- and Cl−-dependent ... "Polymorphisms in the amino acid transporter solute carrier family 6 (neurotransmitter transporter) member 14 gene contribute to ... Sloan JL, Grubb BR, Mager S (2003). "Expression of the amino acid transporter ATB 0+ in lung: possible role in luminal protein ...
... cationic amino acid transporter 2 MeSH D12.776.157.530.200.374.750 -- amino acid transport system y+l MeSH D12.776.157.530. ... amino acid transport system y+ MeSH D12.776.157.530.200.374.600.200 -- cationic amino acid transporter 1 MeSH D12.776.157.530. ... excitatory amino acid transporter 2 MeSH D12.776.157.530.450.625.147.875 -- excitatory amino acid transporter 3 MeSH D12.776. ... excitatory amino acid transporter 4 MeSH D12.776.157.530.450.625.147.968 -- excitatory amino acid transporter 5 MeSH D12.776. ...
... a cationic amino acid transporter. When cells became stressed, the nuclear non-coding RNA levels were reduced, coupled with an ... In anatomy, a paraspeckle is an irregularly shaped compartment of the cell, approximately 0.2-1 μm in size, found in the ... 12 (1): 13-25. doi:10.1016/S0960-9822(01)00632-7. PMID 11790299. Fox, Archa; Wendy Bickmore (2004). "Nuclear Compartments: ... 2004). "Expression and Functional Significance of Mouse Paraspeckle Protein 1 on spermatogenesis". Biology of Reproduction. 71 ...
Solute carrier family 7 (cationic amino acid transporter, y+ system), member 9 also known as SLC7A9 is a protein which in ... Heterodimeric amino acid transporter Solute carrier family "SLC7A9". Feliubadaló L, Font M, Purroy J, Rousaud F, Estivill X, ... This gene encodes a protein that belongs to a family of light subunits of amino acid transporters. This protein plays a role in ... a disease that leads to cystine stones in the urinary system due to impaired transport of cystine and dibasic amino acids. ...
The Cationic Amino Acid Transporter (CAT) Family 2.A.3.4: The Amino Acid/Choline Transporter (ACT) Family 2.A.3.5: The ... The Amino Acid Transporter (AAT) Family 2.A.3.2: The Basic Amino Acid/Polyamine Antiporter (APA) Family 2.A.3.3: ... "The amino acid/polyamine/organocation (APC) superfamily of transporters specific for amino acids, polyamines and organocations ... are amino acid receptors rather than transporters and are truncated at their C-termini, relative to the transporters, having 10 ...
... cationic amino acid transporter/glycoprotein-associatedcationic amino acid transporters (SLC7A1, SLC7A2, SLC7A3, SLC7A4) ... copper transporter(SLC31A1, SLC31A2) (32) vesicular inhibitory amino acid transporter(SLC32A1) (33) Acetyl-CoA transporter( ... Ammonia transporter(RhAG, RhBG, RhCG) (43) Na+-independent, system-L like amino acid transporter (SLC43A1, SLC43A2, SLC43A3) ( ... heavy subunits of heterodimeric amino acid transporters(SLC3A1, SLC3A2) (4) bicarbonate transporter(SLC4A1, SLC4A2, SLC4A3, ...
Folding control of CPP using unnatural β, δ cyclic amino acids. CPPs found applications as transporters of contrast agents ... In the absence of linker, the cationic peptide can interact more efficient with the target cell and cellular uptake occurs ... CPPs typically have an amino acid composition that either contains a high relative abundance of positively charged amino acids ... a natural degradation process by which peptide bonds are hydrolyzed to amino acids. Unnatural acid insertion in the peptide ...
3.A.1.3 Polar Amino Acid Uptake Transporter (PAAT) 3.A.1.4 Hydrophobic Amino Acid Uptake Transporter (HAAT) 3.A.1.5 Peptide/ ... Pgp is known to transport organic cationic or neutral compounds. A few ABCC family members, also known as MRP, have also been ... Opine/Nickel Uptake Transporter (PepT) 3.A.1.6 Sulfate/Tungstate Uptake Transporter (SulT) 3.A.1.7 Phosphate Uptake Transporter ... Nickel Uptake Transporter (NiT) Methionine Uptake Transporter (MUT) 2.A.52 Nickel/Cobalt Uptake Transporter (NiCoT) 3.A.1.106 ...
The attachment of the amino acid lysine slows down the relative amount of dextroamphetamine available to the blood stream. ... especially the dopamine transporter (DAT) and less so the norepinephrine (NET) and serotonin transporter. The mechanism of ... Conversely, an acidic pH means the drug is predominantly in a water-soluble cationic (salt) form, and less is absorbed. ... Malenka RC, Nestler EJ, Hyman SE (2009). "Chapter 5: Excitatory and Inhibitory Amino Acids". In Sydor A, Brown RY. Molecular ...
CPPs typically have an amino acid composition that either contains a high relative abundance of positively charged amino acids ... This feature requires the orientation of cationic -hydrophilic on one side, and hydrophobic residues on the other side of the ... CPPs found applications as transporters of contrast agents across plasma membranes. These contrast agents are able to label the ... a natural degradation process by which peptide bonds are hydrolyzed to amino acids. Unnatural acid insertion in the peptide ...
M3). A cationic amino acid at position 2 is largely responsible for the inhibiting effect of S107. The ratio of S105 to S107 ... Molecular and Cellular Biology portal As of 10 March 2016, this article is derived in whole or in part from Transporter ... The latter protein, S107, is a 2-amino acid extension of the former protein, S105, due to a different translational initiation ... Like the N-terminal 2 amino acid extension in S107, they influence the timing of lysis by a charge dependent mechanism. ...
Through a study done at the University of Albany, it was shown that the ability of the Coomassie dye to target amino acids with ... even under acid conditions when most of the molecules in solution are in the cationic form.[7] This is the basis of the ... This provides a simpler method for fingerprint analysis by reducing the number of amino acids needed to be analyzed from 23 to ... A protein sample is added to a solution of the dye in phosphoric acid and ethanol. Under the acid conditions the dye is ...
Low affinity cationic amino acid transporter 2 is a protein that in humans is encoded by the SLC7A2 gene. SLC7A2 protein, human ... 1997). "Human cationic amino acid transporters hCAT-1, hCAT-2A, and hCAT-2B: three related carriers with distinct transport ... Hammermann R, Brunn G, Racké K (2002). "Analysis of the genomic organization of the human cationic amino acid transporters CAT- ... Schnorr O, Suschek CV, Kolb-Bachofen V (2003). "The importance of cationic amino acid transporter expression in human skin". J ...
N2 - BACKGROUND: l-Arginine transport mediated by type 2 cationic amino acid transporter (CAT-2) is one crucial mechanism that ... AB - BACKGROUND: l-Arginine transport mediated by type 2 cationic amino acid transporter (CAT-2) is one crucial mechanism that ... BACKGROUND: l-Arginine transport mediated by type 2 cationic amino acid transporter (CAT-2) is one crucial mechanism that ... abstract = "BACKGROUND: l-Arginine transport mediated by type 2 cationic amino acid transporter (CAT-2) is one crucial ...
Background: l-arginine transport mediated by type-2 cationic amino acid transporter (CAT-2) isozymes is one crucial mechanism ... N2 - Background: l-arginine transport mediated by type-2 cationic amino acid transporter (CAT-2) isozymes is one crucial ... AB - Background: l-arginine transport mediated by type-2 cationic amino acid transporter (CAT-2) isozymes is one crucial ... abstract = "Background: l-arginine transport mediated by type-2 cationic amino acid transporter (CAT-2) isozymes is one crucial ...
Thrombin stimulates vascular smooth muscle cell polyamine synthesis by inducing cationic amino acid transporter and ornithine ... Platelet-derived growth factor regulates vascular smooth muscle cell proliferation by inducing cationic amino acid transporter ... Lysophosphatidylcholine regulates cationic amino acid transport and metabolism in vascular smooth muscle cells: role in ... Moreover, treatment of VSMCs with BEC or L-OHNA, or knockdown of arginase I protein, arrested cells in the G0/G1 phase of the ...
urinary excretion of cationic amino acids (lysine, arginine, ornithine) decreased blood levels of cationic amino acids ... amino acid transmembrane transporter activity. GO:0015171 9.1. SLC3A1 SLC7A2 SLC7A5 SLC7A6 SLC7A7 SLC7A8 ... L-amino acid transport. GO:0015807 9.56. SLC7A5 SLC7A6 SLC7A7 SLC7A8 4. L-alpha-amino acid transmembrane transport. GO:1902475 ... amino acid transmembrane transport. GO:0003333 9.49. SLC7A2 SLC7A7 8. neutral amino acid transport. GO:0015804 9.48. SLC7A5 ...
1998) Transporters for cationic amino acids in animal cells: discovery, structure, and function Physiol Rev 78,487-545 [PubMed] ... large neutral amino acid transporter, LAT1 and LAT2 (L-type amino acid transporter 1 and 2 respectively), have recently been ... 25 26 27 28 29 30 31 32 System L is a major amino acid transporter that transports large neutral amino acids in a Na+- ... Uchino, H, Kanai, Y, Kim, DK, et al (2002) Transport of amino acid-related compounds mediated by L-type amino acid transporter ...
... low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic ... Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family ... High affinity cationic amino acid transporter 1Add BLAST. 629. Amino acid modifications. Feature key. Position(s). Description ... High affinity cationic amino acid transporter 1. Short name: CAT-11 Publication. ,p>Manually curated information that is based ...
solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 ... IPR002293 Amino acid/polyamine transporter I. IPR029485 Cationic amino acid transporter, C-terminal ... 1 human;1 mouse;1 rat;1 chimpanzee;1 cattle;1 dog;1 chicken;1 zebrafish;1 frog, western clawed;1 macaque, rhesus. ... 12 phenotypes from 1 allele in 1 genetic background 10 phenotype references ...
Reaktivität: Rind (Kuh), Hund, Meerschweinchen and more. 57 verschiedene SLC7A1 Antikörper vergleichen. Alle direkt auf antikörper-online bestellbar!
HCA RNA Cell Line for High affinity cationic amino acid transporter 1. ... High-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in ... Compartment GO Terms for High affinity cationic amino acid transporter 1. Cell 16,178 ... Amino acid and oligopeptide SLC transpor... 52 Amino acid transport across the plasma m... 32 ...
... in one well of a 6-well plate was used to transduce 105 Jurkat T cells expressing the murine cationic amino acid transporter 1 ... Although the Dbs clone isolated was N-terminally truncated from amino acids 1-529, others have shown that amino acids 525-1097 ... After extensive washing, Jurkat cells were treated with 3 ng/ml phorbol myristic acid (Sigma) and 12.5 nM ionomycin (Sigma) for ... TNF receptor 1;. FADD,. Fas-associated death domain protein;. c-FLIP,. cellular FLICE-like inhibitory protein;. GFP,. green ...
high affinity cationic amino acid transporter 1. Names. CAT1. amino acid transporter, cationic 1. ecotropic retroviral leukemia ... L-homoarginine is a substrate of the cationic amino acid transporters CAT1, CAT2A and CAT2B. Title: The prognostic biomarker L- ... Overexpression of cationic amino acid transporter-1 increases nitric oxide production in hypoxic human pulmonary microvascular ... Title: Overexpression of cationic amino acid transporter-1 increases nitric oxide production in hypoxic human pulmonary ...
... cationic amino acid transporter (L-arginine transporter); ASC, sodium-dependent neutral amino acid transporter (L-cysteine ... depriving the environment of amino acids (top right), releasing oxidizing molecules (bottom left), and/or altering T cell ... Gabrilovich DI1, Ostrand-Rosenberg S, Bronte V.. Author information. 1. H. Lee Moffitt Cancer Center and Research Institute, ... transporter); IFNγ, interferon-γ; IL, interleukin; MYD88, myeloid differentiation primary response protein 88; HIF-1α, hypoxia ...
RecName: Full=High affinity cationic amino acid transporter 1; Short=CAT-1 {ECO:0000250,UniProtKB:P30825}; Short=CAT1;AltName: ... solute carrier family 7 (cationic amino acid transporter, y+ system), member 1. 21. 4. 9. 94. ... J:11385 Kim JW, et al., Transport of cationic amino acids by the mouse ecotropic retrovirus receptor [see comments]. Nature. ... The Mitochondrial Metallochaperone SCO1 Is Required to Sustain Expression of the High-Affinity Copper Transporter CTR1 and ...
... cationic amino acid transporter 3; (O) D52-like 2; (P) human KIAA0147. (Q-X) Transverse sections, dorsal up. (Q) cathepsin D; ( ... but only neuropilin-2 functions as a receptor for the 145-amino acid form of VEGF. J. Biol. Chem. 275,18040 -18045. ... GCN20/ATP-binding cassette transporter F2; (H) tyrosine kinase receptor KLG; (I) MSE55; (J) dyskerin; (K) thimet oligopeptidase ... 1. Neural crest induction in vitro. (A) Non-neural ectoderm (open blue rectangle) and intermediate neural plate (solid blue ...
AA 1-50 for FACS, ICC, IF, IHC, IHC (p), SimWes, WB. Published in 20 Pubmed References. Order this anti-SLC7A11 antibody. , ... Cationic Amino Acid Transporter,... SLC7A11 antibody (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) ... cationic amino acid transporter, y+ system) member 11 * solute carrier family 7 (cationic amino acid transporter, y+ system), ... Cationic Amino Acid Transporter, Y+ System), Member 9 Antibodies * anti-Solute Carrier Family 7 (Cationic Amino Acid ...
The transport of L-arginine occurs through the cationic amino acid transporter CAT-1. Urea transport occurs through urea ... Nitric oxide synthesis relies on the amino acid substrate L-arginine. L-arginine synthesis primarily occurs in the proximal ... The maintenance of plasma L-arginine levels in CKD may be a consequence of increased amino acid release into the blood due to ... Nitric oxide is produced from the amino acid substrate L-arginine by the enzyme endothelial nitric oxide synthase (eNOS) [18]. ...
solute carrier family 7 (cationic amino acid transporter, y+ system), member 4. 6. 7. 9. 1. ... cationic amino acid transporter, y+ system), member 4 ... Nucleic Acids Research (Journal) Database Summary. *Mouse Facts ... pep:known chromosome:VEGA66:16:17572018:17576721:-1 gene:OTTMUSG00000035925 transcript:OTTMUST00000092151 gene_biotype:protein_ ...
B(0)AT basic neutral amino acid transporter, OAT organic amino acid transporter, CAT cationic amino acid transporter, LAT L ... The major endothelial transporter for L -Arg is the system y+ cation ic amino acid transporter (CAT). CAT1 is constitutively ... Cationic amino acid transporter (CAT) has been shown to be the major transporter for L-Arg and function in L-Arg secretion by ... Transport Cationic Amino Acid Transporter L -Arg that is produced by the proximal tubule must be secreted in to the circulation ...
... cystine transporter, cationic amino acid transporter 1, and large amino acid transporter 1, respectively, were monitored by ... ATF4 is the master regulator of amino acid metabolism and is activated by amino acid deprivation (18). Levels of amino acids ... a transporter for positively charged amino acids; and LAT1, a transporter for large amino acids. Similar to CSE, these genes ... We monitored transcriptional targets of ATF4, such as the genes for xCT, the transporter for cystine; cationic amino acid ...
... cationic amino acid transporter". Kaira K, Oriuchi N, Imai H, et al. (2009). "Prognostic significance of L-type amino acid ... 2009). "L-type amino acid transporter 1 (LAT1) is frequently expressed in thymic carcinomas but is absent in thymomas". J Surg ... 2009). "L-type amino-acid transporter 1 as a novel biomarker for high-grade malignancy in prostate cancer". Pathol. Int. 59 (1 ... 2006). "L-type amino acid transporter 1 as a potential molecular target in human astrocytic tumors". Int. J. Cancer. 119 (3): ...
Cationic amino acid transporters play key roles in the survival and transmission of apicomplexan parasites ... Genetic Mapping Reveals that Sinefungin Resistance in Toxoplasma gondii Is Controlled by a Putative Amino Acid Transporter ... The aromatic amino acid hydroxylase genes AAH1 and AAH2 in Toxoplasma gondii contribute to transmission in the cat ... The Adenosine Transporter ofToxoplasma gondii. Chi-Wu Chiang, Nicola Carter, William J. Sullivan, Robert G. K. Donald, David S ...
Cationic amino acid transporter (CAT)-1 mRNA remained little changed, and CAT-2 mRNA was not detected. The plasma nitrogen ... Oyadomari S1, Gotoh T, Aoyagi K, Araki E, Shichiri M, Mori M. ... eNOS and AS mRNAs were increased by three- to fourfold 1-2 ... 1. Department of Molecular Genetics, Kumamoto University School of Medicine, Kumamoto 860-0811, Japan.. ... oxide levels were increased 1-2 weeks after STZ treatment and decreased at 4 weeks. Transforming growth factor-beta1 (TGF-beta1 ...
Currents of Opposite Polarity Evoked by Neutral and Cationic Amino Acids in Neutral and Basic Amino Acid Transporter cRNA- ... amino acid transport on substrate affinity of the heteromeric b(0,+) amino acid transporter". J. Biol. Chem. 275 (19): 14331- ... "Effects of truncation of the COOH-terminal region of a Na+-independent neutral and basic amino acid transporter on amino acid ... dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1". Pras E ...
Independently reviewed in 1 review(s). Immunogen corresponding to synthetic peptide. ... Sodium independent neutral amino acid transporter LAT1 antibody. *Solute carrier family 7 (cationic amino acid transporter y+ ... Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. ... Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood- ...
  • BACKGROUND: l-Arginine transport mediated by type 2 cationic amino acid transporter (CAT-2) is one crucial mechanism that regulates nitric oxide production mediated by inducible nitric oxide synthase. (elsevier.com)
  • The authors sought to explore the effects of HO-1 induction on CAT-2 expression and l-arginine transport. (elsevier.com)
  • CONCLUSIONS: HO-1 induction significantly inhibited CAT-2 expression and l-arginine transport in lipopolysaccharide-stimulated macrophages, possibly through mechanisms involved activation of Nrf2 and inhibition of NF-κB. (elsevier.com)
  • In addition, carbon monoxide mediated, at least in part, the effects of HO-1 induction on CAT-2 expression and l-arginine transport. (elsevier.com)
  • Results: LPS increased HO activity, HO-1 concentration, NO production, l-arginine transport, and concentrations of iNOS, CAT-2, and CAT-2B in rat lungs and kidney. (elsevier.com)
  • Conclusions: HO-1 overexpression significantly attenuates endotoxin-induced increases in NO production and l-arginine transport. (elsevier.com)
  • Interaction of the cardiovascular risk marker asymmetric dimethylarginine (ADMA) with the human cationic amino acid transporter 1 (CAT1). (nih.gov)
  • The genes identified in the screen include both modifiers that only partially protected RelA −/− cells against TNFα and a dominant-negative mutant of TNF receptor 1 (TNFR1) that was generated during the process of CPR. (pnas.org)
  • Transport of cationic amino acids by the mouse ecotropic retrovirus receptor [see comments]. (jax.org)
  • MicroRNA-497 targets insulin-like growth factor 1 receptor and has a tumour suppressive role in human colorectal cancer," Oncogene , vol. 32, no. 15, pp. 1910-1920, 2013. (hindawi.com)
  • Structural requirements for binding of prostaglandins to the prostaglandin FP receptor versus the prostaglandin transporter PGT. (yu.edu)
  • Thus, the current study gave new insights both to the ET-1-induced signal transduction and the internalization mechanism of ET-1/receptor complexes. (nii.ac.jp)
  • Retroviral cDNA libraries have been used in a number of functional screens to identify unique mammalian genes that regulate cellular responses ( 1 - 6 ). (pnas.org)
  • Here we describe an alternative method called cyclical packaging rescue (CPR) that uses direct repackaging of retroviral RNAs into new infectious virions to identify genes regulating functional responses in mammalian cells (Fig. 1 A ). In CPR, stably integrated helper-free retroviral libraries are recovered rapidly from mammalian cells as infectious helper-free retroviral supernatants 24 h after infection with adenoviruses expressing retroviral gag-pol and env genes. (pnas.org)
  • Therefore, in the current study, the expression of SLC37A family genes was evaluated in patients with colorectal cancer (CRC), and it was revealed that SLC family 37 member 1 (SLC37A1) expression was significantly increased in tumorous tissues compared with that in non-tumorous tissues. (readbyqxmd.com)
  • More than 50 distinct amino acid transporter genes have been identified in the genome of Arabidopsis, indicating that transport of amino acids across membranes is a highly complex feature in plants. (xenbase.org)
  • Treatment with rapamycin mimics starvation for nitrogen in S. cerevisiae despite availability of nutrients, resulting in endocytosis and degradation of specific permeases and their substitution by the general amino acid permease, Gap1 ( C respo and H all 2002 ). (genetics.org)
  • The Mitochondrial Metallochaperone SCO1 Is Required to Sustain Expression of the High-Affinity Copper Transporter CTR1 and Preserve Copper Homeostasis. (jax.org)
  • This antiporter imports cystine and exports glutamate, which are both amino acids. (wikipedia.org)
  • If either of these transporters are impaired, it could result in a disruption in glutamate homeostasis and lead to a variety of CNS disorders It has been found that cocaine produces a decrease in Cystine-Glutamate exchange via system Xc-, leading to a decrease in basal, extra synaptic glutamate levels in the nucleus accumbens core (NAcc) region of the brains of cocaine-withdrawn rats. (wikipedia.org)
  • Cystine is the only amino acid that forms crystals and stones in the bladder or kidneys, leading to the signs and symptoms of cystinuria. (nih.gov)
  • 1 Substantial evidence from preclinical studies and human research indicates that impairment of the endothelial NO synthase (NOS) pathway accelerates vascular disease and increases the risk for major adverse cardiovascular events. (ahajournals.org)
  • Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. (abcam.com)
  • Aromatic amino acid:H + symporter, AroP of 457 aas and 12 TMSs ( Cosgriff and Pittard 1997 ). (tcdb.org)
  • RT-PCR analysis confirmed that HCMV induced the expression of ICAM-1, integrin β 8 , integrin α 1 , IL-1β, TNF-α, and CCL2. (jimmunol.org)
  • A colorectal cancer expression profile that includes transforming growth factor β inhibitor BAMBI predicts metastatic potential," Gastroenterology , vol. 137, no. 1, pp. 165-175, 2009. (hindawi.com)
  • KRAS up-regulates the expression of miR-181a, miR-200c and miR-210 in a three-dimensional-specific manner in DLD-1 colorectal cancer cells," Anticancer Research , vol. 32, no. 6, pp. 2271-2276, 2012. (hindawi.com)
  • Adding hemin to the preparations, they found, enhanced the lipopolysaccharide-induced nuclear factor-E2-related factor 2 activation and HO-1 expression, in a dose-dependent manner. (asahq.org)
  • miR-122 expression is specific to the liver, where it has been implicated as a regulator of fatty-acid metabolism in mouse studies. (mirbase.org)
  • Die Expression von mRNA für iNOS und CAT-2B in Rattenalveolarmakrophagen ist durch AP-1 und LREAA-decoy-ODN verschieden beeinflußt. (uni-bonn.de)
  • l -Arg depletion by tumor-associated myeloid cells blocked the re-expression of CD3ζ in stimulated T cells and inhibited antigen-specific proliferation of OT-1 and OT-2 cells. (aacrjournals.org)
  • Sloan JL, Mager S. Cloning and functional expression of a human Na(+) and Cl(-)-dependent neutraland cationic amino acid transporter B(0+).J Biol Chem. (tcdb.org)
  • Transfection of astrocyte cultures was performed using a pLEX expression vector carrying the human HO-1 sequence prior to IL-1β treatment. (pubmedcentralcanada.ca)
  • When pretreated with SnPP, the inhibitory effect of hemin on IL-1β-induced NO production and iNOS expression was reversed, suggesting the involvement of HO-1. (pubmedcentralcanada.ca)
  • These findings support the hypothesis that up-regulation of HO-1 in astrocytes is associated with down-regulation of iNOS expression and thereby NO production, an effect that involves the p38 MAPK signaling pathway, which suggests that this glial cell response could play an important protective role against oxidative stress in the brain. (pubmedcentralcanada.ca)
  • Induction of HO-1 expression within the central nervous system (CNS) has been demonstrated in rodent astrocytes, microglia/macrophages and neurons [ 6 - 8 ]. (pubmedcentralcanada.ca)
  • In the current study, we focused on the ET-1-activated signal transduction pathways in cancer cells and found that ET-1, via activation of ETB receptors, induced expression of p53 and apoptotic cell death of A375 melanoma cells. (nii.ac.jp)
  • However, the expression and role of SLC transporters in malignant tumors has not yet been fully elucidated. (readbyqxmd.com)
  • RESULTS: Lipopolysaccharide significantly induced Nrf2 activation and HO-1 expression. (elsevier.com)
  • In a dose-dependent manner, hemin enhanced the lipopolysaccharide-induced Nrf2 activation and HO-1 expression. (elsevier.com)
  • Induction of HO-1 overexpression also significantly attenuates the effects of endotoxin on the expression of iNOS and CAT-2 isozymes in septic rats. (elsevier.com)
  • Huang, TY , Tsai, PS & Huang, CJ 2008, ' HO-1 Overexpression Attenuates Endotoxin Effects on CAT-2 Isozymes Expression ', Journal of Surgical Research , vol. 148, no. 2, pp. 172-180. (elsevier.com)
  • The expression profiles suggest that CAT5 may function in reuptake of leaking amino acids at the leaf margin, while CAT8 is expressed in young and rapidly dividing tissues such as young leaves and root apical meristem. (xenbase.org)
  • Most tumor cells exhibit obligatory demands for essential amino acids (EAAs), but the regulatory mechanisms whereby tumor cells take up EAAs and EAAs promote malignant transformation remain to be determined. (readbyqxmd.com)
  • To ensure that positive cDNA clones are successfully isolated from complex libraries in a single round of selection, functional screens using mammalian cells have been restricted to cellular assays in which the background of false-positive cells meeting a selection criterion is low, typically 1 in 10 5 false-positive cells. (pnas.org)
  • Overexpression of Ca 2+ pump SERCA1, Ca 2+ /H + antiporter Vcx1, or a Mn 2+ transporting mutant of Vcx1 (Vcx1-M1) failed to restore rapamycin sensitivity, and loss of Pmr1 but not other transporters of Ca 2+ or Mn 2+ results in rapamycin resistance. (genetics.org)
  • Overexpression of Ccc1, a Fe 2+ and Mn 2+ transporter that has been localized to Golgi and the vacuole, does restore rapamycin sensitivity to pmr1 Δ. (genetics.org)
  • We sought to investigate the effects of heme oxygenase-1 (HO-1) overexpression on CAT-2 isozymes, e.g. (elsevier.com)
  • Hemin further increased HO activity and induced HO-1 overexpression in the lungs, kidney, and liver from LPS-treated rats. (elsevier.com)