Catenins. Medical search

A family of cytoskeletal proteins that play essential roles in CELL ADHESION at ADHERENS JUNCTIONS by linking CADHERINS to the ACTIN FILAMENTS of the CYTOSKELETON.

A catenin that binds F-ACTIN and links the CYTOSKELETON with BETA CATENIN and GAMMA CATENIN.

A multi-functional catenin that is highly homologous to BETA CATENIN. Gamma catenin binds CADHERINS and helps link their cytoplasmic tails to ACTIN in the CYTOSKELETON via ALPHA CATENIN. It is also found in DESMOSOMES where it mediates the link between DESMOSOMAL CADHERINS and DESMOPLAKIN.

Desmoplakins are cytoskeletal linker proteins that anchor INTERMEDIATE FILAMENTS to the PLASMA MEMBRANE at DESMOSOMES.

Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body.

A multi-functional catenin that participates in CELL ADHESION and nuclear signaling. Beta catenin binds CADHERINS and helps link their cytoplasmic tails to the ACTIN in the CYTOSKELETON via ALPHA CATENIN. It also serves as a transcriptional co-activator and downstream component of WNT PROTEIN-mediated SIGNAL TRANSDUCTION PATHWAYS.

Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.

Anchoring points where the CYTOSKELETON of neighboring cells are connected to each other. They are composed of specialized areas of the plasma membrane where bundles of the ACTIN CYTOSKELETON attach to the membrane through the transmembrane linkers, CADHERINS, which in turn attach through their extracellular domains to cadherins in the neighboring cell membranes. In sheets of cells, they form into adhesion belts (zonula adherens) that go all the way around a cell.

Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.

Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.

Direct contact of a cell with a neighboring cell. Most such junctions are too small to be resolved by light microscopy, but they can be visualized by conventional or freeze-fracture electron microscopy, both of which show that the interacting CELL MEMBRANE and often the underlying CYTOPLASM and the intervening EXTRACELLULAR SPACE are highly specialized in these regions. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p792)

Adherence of cells to surfaces or to other cells.

A family of proteins that contain several 42-amino acid repeat domains and are homologous to the Drosophila armadillo protein. They bind to other proteins through their armadillo domains and play a variety of roles in the CELL including SIGNAL TRANSDUCTION, regulation of DESMOSOME assembly, and CELL ADHESION.

A group of desmosomal cadherins with cytoplasmic tails that resemble those of classical CADHERINS.

The phenomenon by which dissociated cells intermixed in vitro tend to group themselves with cells of their own type.

Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.

A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS.

The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.

Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.

Proteins obtained from various species of Xenopus. Included here are proteins from the African clawed frog (XENOPUS LAEVIS). Many of these proteins have been the subject of scientific investigations in the area of MORPHOGENESIS and development.

Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)

Established cell cultures that have the potential to propagate indefinitely.

Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.

Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.

The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.

The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.

Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

Histochemical localization of immunoreactive substances using labeled antibodies as reagents.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.

Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.

Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.

Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.

Misexpression of the catenin p120(ctn)1A perturbs Xenopus gastrulation but does not elicit Wnt-directed axis specification. (1/407)

Modulators of cadherin function are of great interest given that the cadherin complex actively contributes to the morphogenesis of virtually all tissues. The catenin p120(ctn) (formerly p120cas) was first identified as a src- and receptor-protein tyrosine kinase substrate and later shown to interact directly with cadherins. In common with beta-catenin and plakoglobin (gamma-catenin), p120(ctn) contains a central Armadillo repeat region by which it binds cadherin cytoplasmic domains. However, little is known about the function of p120(ctn) within the cadherin complex. We examined the role of p120(ctn)1A in early vertebrate development via its exogenous expression in Xenopus. Ventral overexpression of p120(ctn)1A, in contrast to beta-catenin, did not induce the formation of duplicate axial structures resulting from the activation of the Wnt signaling pathway, nor did p120(ctn) affect mesoderm induction. Rather, dorsal misexpression of p120(ctn) specifically perturbed gastrulation. Lineage tracing of cells expressing exogenous p120(ctn) indicated that cell movements were disrupted, while in vitro studies suggested that this may have been a consequence of reduced adhesion between blastomeres. Thus, while cadherin-binding proteins beta-catenin, plakoglobin, and p120(ctn) are members of the Armadillo protein family, it is clear that these proteins have distinct biological functions in early vertebrate development. This work indicates that p120(ctn) has a role in cadherin function and that heightened expression of p120(ctn) interferes with appropriate cell-cell interactions necessary for morphogenesis. (+info)

The catenin p120(ctn) interacts with Kaiso, a novel BTB/POZ domain zinc finger transcription factor. (2/407)

p120(ctn) is an Armadillo repeat domain protein with structural similarity to the cell adhesion cofactors beta-catenin and plakoglobin. All three proteins interact directly with the cytoplasmic domain of the transmembrane cell adhesion molecule E-cadherin; beta-catenin and plakoglobin bind a carboxy-terminal region in a mutually exclusive manner, while p120 binds the juxtamembrane region. Unlike beta-catenin and plakoglobin, p120 does not interact with alpha-catenin, the tumor suppressor adenomatous polyposis coli (APC), or the transcription factor Lef-1, suggesting that it has unique binding partners and plays a distinct role in the cadherin-catenin complex. Using p120 as bait, we conducted a yeast two-hybrid screen and identified a novel transcription factor which we named Kaiso. Kaiso's deduced amino acid sequence revealed an amino-terminal BTB/POZ protein-protein interaction domain and three carboxy-terminal zinc fingers of the C2H2 DNA-binding type. Kaiso thus belongs to a rapidly growing family of POZ-ZF transcription factors that include the Drosophila developmental regulators Tramtrak and Bric a brac, and the human oncoproteins BCL-6 and PLZF, which are causally linked to non-Hodgkins' lymphoma and acute promyelocytic leukemia, respectively. Monoclonal antibodies to Kaiso were generated and used to immunolocalize the protein and confirm the specificity of the p120-Kaiso interaction in mammalian cells. Kaiso specifically coprecipitated with a variety of p120-specific monoclonal antibodies but not with antibodies to alpha- or beta-catenin, E-cadherin, or APC. Like other POZ-ZF proteins, Kaiso localized to the nucleus and was associated with specific nuclear dots. Yeast two-hybrid interaction assays mapped the binding domains to Arm repeats 1 to 7 of p120 and the carboxy-terminal 200 amino acids of Kaiso. In addition, Kaiso homodimerized via its POZ domain but it did not heterodimerize with BCL-6, which heterodimerizes with PLZF. The involvement of POZ-ZF proteins in development and cancer makes Kaiso an interesting candidate for a downstream effector of cadherin and/or p120 signaling. (+info)

p120(ctn) acts as an inhibitory regulator of cadherin function in colon carcinoma cells. (3/407)

p120(ctn) binds to the cytoplasmic domain of cadherins but its role is poorly understood. Colo 205 cells grow as dispersed cells despite their normal expression of E-cadherin and catenins. However, in these cells we can induce typical E-cadherin-dependent aggregation by treatment with staurosporine or trypsin. These treatments concomitantly induce an electrophoretic mobility shift of p120(ctn) to a faster position. To investigate whether p120(ctn) plays a role in this cadherin reactivation process, we transfected Colo 205 cells with a series of p120(ctn) deletion constructs. Notably, expression of NH2-terminally deleted p120(ctn) induced aggregation. Similar effects were observed when these constructs were introduced into HT-29 cells. When a mutant N-cadherin lacking the p120(ctn)-binding site was introduced into Colo 205 cells, this molecule also induced cell aggregation, indicating that cadherins can function normally if they do not bind to p120(ctn). These findings suggest that in Colo 205 cells, a signaling mechanism exists to modify a biochemical state of p120(ctn) and the modified p120(ctn) blocks the cadherin system. The NH2 terminus-deleted p120(ctn) appears to compete with the endogenous p120(ctn) to abolish the adhesion-blocking action. (+info)

Functional characterization of multiple transactivating elements in beta-catenin, some of which interact with the TATA-binding protein in vitro. (4/407)

beta-Catenin, a member of the family of Armadillo repeat proteins, plays a dual role in cadherin-mediated cell adhesion and in signaling by Wnt growth factors. Upon Wnt stimulation beta-catenin undergoes nuclear translocation and serves as transcriptional coactivator of T cell factor DNA-binding proteins. Previously the transactivation potential of different portions of beta-catenin has been demonstrated, but the precise location of transactivating elements has not been established. Also, the mechanism of transactivation by beta-catenin and the molecular basis for functional differences between beta-catenin and the closely related proteins Armadillo and Plakoglobin are poorly understood. Here we have used a yeast system for the detailed characterization of the transactivation properties of beta-catenin. We show that its transactivation domains possess a modular structure, consist of multiple subelements that cover broad regions at its N and C termini, and extend considerably into the Armadillo repeat region. Compared with beta-catenin the N termini of Plakoglobin and Armadillo have different transactivation capacities that may explain their distinct signaling properties. Furthermore, transactivating elements of beta-catenin interact specifically and directly with the TATA-binding protein in vitro providing further evidence that a major function of beta-catenin during Wnt signaling is to recruit the basal transcription machinery to promoter regions of Wnt target genes. (+info)

Nuclear localization of the p120(ctn) Armadillo-like catenin is counteracted by a nuclear export signal and by E-cadherin expression. (5/407)

The Armadillo protein p120(ctn) associates with the cytoplasmic domain of cadherins and accumulates at cell-cell junctions. Particular Armadillo proteins such as beta-catenin and plakophilins show a partly nuclear location, suggesting gene-regulatory activities. For different human E-cadherin-negative carcinoma cancer cell lines we found expression of endogenous p120(ctn) in the nucleus. Expression of E-cadherin directed p120(ctn) out of the nucleus. Previously, we reported that the human p120(ctn) gene might encode up to 32 protein isoforms as products of alternative splicing. Overexpression of p120(ctn) isoforms B in various cell lines resulted in cytoplasmic immunopositivity but never in nuclear staining. In contrast, upon expression of p120(ctn) cDNAs lacking exon B, the isoforms were detectable within both nuclei and cytoplasm. A putative nuclear export signal (NES) with a characteristic leucine-rich motif is encoded by exon B. This sequence element was shown to be required for nuclear export and to function autonomously when fused to a carrier protein and microinjected into cell nuclei. Moreover, the NES function of endogenously or exogenously expressed p120(ctn) isoforms B was sensitive to the nuclear export inhibitor leptomycin B. Expression of exogenous E-cadherin down-regulated nuclear p120(ctn) whereas activation of protein kinase C increased the level of nuclear p120(ctn). These results reveal molecular mechanisms controlling the subcellular distribution of p120(ctn). (+info)

p120(ctn) binds to the membrane-proximal region of the E-cadherin cytoplasmic domain and is involved in modulation of adhesion activity. (6/407)

Cadherins are transmembrane glycoproteins involved in Ca(2+)-dependent cell-cell adhesion. Previously, we showed that the conserved membrane-proximal region of the E-cadherin cytoplasmic domain negatively regulates adhesion activity. In this report, we provide several lines of evidence that p120(ctn) is involved in this negative regulation. p120(ctn) binds to the membrane-proximal region of the nonfunctional carboxyl-terminally deleted E-cadherin protein. An additional internal deletion in this region prevented the association with p120(ctn) and activated the protein, as seen in an aggregation assay. Furthermore, the nonfunctional E-cadherin can be activated through coexpression of p120(ctn) proteins with amino-terminal deletions, which eliminate several potential serine/threonine phosphorylation sites but do not affect the ability to bind to cadherins. Finally, we show that staurosporine, a kinase inhibitor, induces an increased electrophoretic mobility of p120(ctn) bound to E-cadherin polypeptides, activates the nonfunctional E-cadherin protein, and converts the wild-type E-cadherin and an E-cadherin-alpha-catenin chimeric protein from a cytochalasin D-sensitive to a cytochalasin D-insensitive state. Together, these results indicate that p120(ctn) is a modulator of E-cadherin-mediated cell adhesion. (+info)

N-Cadherin expression in human prostate carcinoma cell lines. An epithelial-mesenchymal transformation mediating adhesion withStromal cells. (7/407)

In human prostate adenocarcinoma, an association between loss of E-cadherin, increased Gleason score, and extracapsular dissemination has been observed. Further characterization of the E-cadherin/catenin phenotype of human prostate carcinoma cell lines showed loss of E-cadherin and expression of N-cadherin in poorly differentiated prostate carcinoma cell lines (PC-3N derived from PC-3, PC-3, and JCA1). We showed that N-cadherin is concentrated at sites of cell-cell contact in PC-3N cellular extensions. N-cadherin was also expressed in prostate stromal fibroblasts both in vitro and in prostate tissue. Co-cultures of prostate stromal fibroblasts and PC-3N cells showed the immunolocalization of N-cadherin in intercellular contacts. In addition, the isoform expression of the cadherin binding protein p120(ctn) differed in relation to the expression of E- versus N-cadherin by the prostate carcinoma cell lines. The p100 isoform was more highly expressed in E-cadherin-positive carcinoma cell lines, whereas p120 was predominantly expressed only in N-cadherin-positive prostate carcinoma cell lines and prostate stromal fibroblasts. The N-cadherin-positive carcinoma cell line, PC-3N, displayed aggressive invasion into the surface of the diaphragm muscle after intraperitoneal injection of SCID mice. The gain of N-cadherin and loss of E-cadherin by invasive prostate carcinoma cell lines suggests a progression from an epithelial to a mesenchymal phenotype, which may allow for their interaction with surrounding stromal fibroblasts and facilitate metastasis. (+info)

Epithelial mesenchymal transition by c-Fos estrogen receptor activation involves nuclear translocation of beta-catenin and upregulation of beta-catenin/lymphoid enhancer binding factor-1 transcriptional activity. (8/407)

Mouse mammary epithelial cells expressing a fusion protein of c-Fos and the estrogen receptor (FosER) formed highly polarized epithelial cell sheets in the absence of estradiol. Beta-catenin and p120(ctn) were exclusively located at the lateral plasma membrane in a tight complex with the adherens junction protein, E-cadherin. Upon activation of FosER by estradiol addition, cells lost epithelial polarity within two days, giving rise to a uniform distribution of junctional proteins along the entire plasma membrane. Most of the beta-catenin and p120(ctn) remained in a complex with E-cadherin at the membrane, but a minor fraction of uncomplexed cytoplasmic beta-catenin increased significantly. The epithelial-mesenchymal cell conversion induced by prolonged estradiol treatment was accompanied by a complete loss of E-cadherin expression, a 70% reduction in beta-catenin protein level, and a change in the expression pattern of p120(ctn) isoforms. In these mesenchymal cells, beta-catenin and p120(ctn) were localized in the cytoplasm and in defined intranuclear structures. Furthermore, beta-catenin colocalized with transcription factor LEF-1 in the nucleus, and coprecipitated with LEF-1-related proteins from cell extracts. Accordingly, beta-catenin- dependent reporter activity was upregulated in mesenchymal cells and could be reduced by transient expression of exogenous E-cadherin. Thus, epithelial mesenchymal conversion in FosER cells may involve beta-catenin signaling. (+info)

The first two catenins that were identified became known as α-catenin and β-catenin. α-Catenin can bind to β-catenin and can ... α-catenin β-catenin γ-catenin δ-catenin All but α-catenin contain armadillo repeats. They exhibit a high degree of protein ... Most studies investigating catenin actions have focused on α-catenin and β-catenin. β-catenin is particularly interesting as it ... Additional catenins such as γ-catenin and δ-catenin have been identified. The name "catenin" was originally selected ('catena' ...

https://en.wikipedia.org/wiki/Catenin

... alpha-2-catenin (also called alpha-N-catenin) CTNNA3, alpha-3-catenin (also called alpha-T-catenin) Catenin alpha+Catenin at ... Three alpha-catenin genes are expressed in humans:[citation needed] CTNNA1, alpha-1-catenin (also called alpha-E-catenin) ... beta-catenin and alpha-catenin has not been isolated.[citation needed] The amino acid sequence of alpha-catenin has sequence ... It has been observed that when alpha-catenin is not in a molecular complex with beta-catenin, it dimerizes and functions to ...

https://en.wikipedia.org/wiki/Alpha_catenin

Like beta-catenin and gamma-catenin, delta-catenins seem to interact with Presenilins. These catenin-presenilin interaction ... Delta-1-catenin and Delta-2-catenin are members of a subfamily of proteins with ten Armadillo-repeats. Delta-2-catenin is ... While beta-catenin acts as a transcription regulatory protein in the Wnt/TCF pathway, delta-catenin 1 has been implicated as a ... Catenin CTNND1 v t e (Articles with short description, Short description matches Wikidata, Protein pages needing a picture, ...

https://en.wikipedia.org/wiki/Delta_catenin

... , also known as beta-catenin (β-catenin), is a protein that in humans is encoded by the CTNNB1 gene. Beta-catenin ... This is possible because α-catenin and cadherins bind at distinct sites to β-catenin. The β-catenin - α-catenin complex can ... On the other hand, BCL9 and BCL9L must compete with α-catenin to access β-catenin molecules. The cellular level of beta-catenin ... It is a member of the catenin protein family and homologous to γ-catenin, also known as plakoglobin. Beta-catenin is widely ...

https://en.wikipedia.org/wiki/Catenin_beta-1

... αE-catenin has no homology to established actin-binding proteins. The N-terminus of αE-catenin binds β-catenin or γ-catenin/ ... αE-catenin, also known as Catenin alpha-1 is a protein that in humans is encoded by the CTNNA1 gene. αE-catenin is highly ... αE-catenin has been shown to interact with: APC, Beta-catenin, CDH1, CDH2, CDH3 Plakoglobin, and VE-cadherin. Alpha catenin ... Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin ...

https://en.wikipedia.org/wiki/Catenin_alpha-1

... α-catenins, β-catenins, and p120ctn family catenins. β-catenins and p120ctns bind cadherin's intracellular domain at the distal ... will associate with the cadherin-catenin complex via β-catenins. In homodimeric form, α-catenins do not bind β- catenins, but ... β-catenin localizes reserve pool vesicles (RPVs) at presynaptic sites. The deletion of β-catenin in vivo results in a decrease ... Catenins also bind many scaffolding proteins, receptors, kinases and phosphatases. For example, the cadherin-α-catenin complex ...

https://en.wikipedia.org/wiki/Cadherin–catenin_complex_in_learning_and_memory

... is a protein that in humans is encoded by the DACT2 gene. GRCh38: Ensembl ... "Entrez Gene: Dishevelled binding antagonist of beta catenin 2". Retrieved 2017-09-10. Koga Y, Yao T, Hirahashi M, Kumashiro Y, ... "DACT2 is a functional tumor suppressor through inhibiting Wnt/β-catenin pathway and associated with poor survival in colon ... "Flat adenoma-carcinoma sequence with high-malignancy potential as demonstrated by CD10 and beta-catenin expression: a different ...

https://en.wikipedia.org/wiki/Dishevelled_binding_antagonist_of_beta_catenin_2

"Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals". Developmental ... Dishevelled binding antagonist of beta catenin 1 (Dact1, previously known as Dapper, Dpr1, Frodo) is a protein that in humans ... "DACT1 dishevelled binding antagonist of beta catenin 1 [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved ... a Dishevelled-Associated Antagonist of β-Catenin and JNK Signaling, Is Required for Notochord Formation". Developmental Cell. 2 ...

https://en.wikipedia.org/wiki/Dishevelled_binding_antagonist_of_beta_catenin_1

Takemaru, Ken-Ichi (2006). "Catenin, beta". AfCS-Nature Molecule Pages. doi:10.1038/mp.a000506.01. Rousseau, Simon (2011). "P38 ...

https://en.wikipedia.org/wiki/Signaling_Gateway_(website)

CTNNB1 (beta-catenin; a transcription gene) mutations are found in 14-44% of endometrial cancers and may indicate a good ... Beta-catenin mutations are commonly found in endometrial cancers with squamous cells. FGFR2 mutations are found in ... The CTNNB1 (beta-catenin) gene is most commonly mutated in the squamous subtype of endometrioid adenocarcinoma. Serous ...

https://en.wikipedia.org/wiki/Endometrial_cancer

"Axin-independent phosphorylation of APC controls beta-catenin signaling via cytoplasmic retention of beta-catenin". Biochem. ... "Entrez Gene: catenin". Tago K, Nakamura T, Nishita M, Hyodo J, Nagai S, Murata Y, Adachi S, Ohwada S, Morishita Y, Shibuya H, ... Beta-catenin-interacting protein 1 is a protein that is encoded in humans by the CTNNBIP1 gene. The protein encoded by this ... Reichel O, Mayr D, Durst F, Berghaus A (2008). "E-cadherin but not beta-catenin expression is decreased in laryngeal biopsies ...

https://en.wikipedia.org/wiki/CTNNBIP1

APC was found to associate with catenins. Today we know that the beta-catenin protein (part of the Wnt signaling pathway) is ... A mouse model with deregulation of beta-catenin levels was created. The conditional stabilizing mutation in the beta-catenin ... there was substantial nuclear localization of beta-catenin as well as increased cytoplasmic beta-catenin. However, in mice fed ... Furthermore, when evaluated for ERCC1, beclin-1, and beta-catenin in the stem cell region of crypts, the colonic tissues of ...

https://en.wikipedia.org/wiki/Mouse_model_of_colorectal_and_intestinal_cancer

Catenins are proteins that associate with the cytoplasmic domain of a variety of cadherins. The association of catenins to ... Three different types of catenins seem to exist: alpha, beta, and gamma. Alpha-catenins are proteins of about 100 kDa which are ... In terms of their structure the most significant differences are the absence, in alpha-catenin, of the repeated domain and of ... Vinculin InterPro: IPR000633 Alpha-catenin InterPro: IPR001033 CTNNA1; CTNNA2; CTNNA3; CTNNAL1; VCL; Otto JJ (1990). "Vinculin ...

https://en.wikipedia.org/wiki/Vinculin_family

Wnt/beta-catenin Pathway. The role of Tbx2 in Wnt signaling has yet to be confirmed; however, up-regulation of Tbx2 in the beta ... catenin signaling pathway leads to loss of the adhesion molecule E-cadherin. This returns cells to a mesenchymal state, and ...

https://en.wikipedia.org/wiki/TBX2

beta-catenin signaling cascade". The Journal of Biological Chemistry. 279 (15): 14879-88. doi:10.1074/jbc.M306421200. PMID ... "Wnt-4 activates the canonical beta-catenin-mediated Wnt pathway and binds Frizzled-6 CRD: functional implications of Wnt/beta- ... catenin activity in kidney epithelial cells". Experimental Cell Research. 298 (2): 369-87. doi:10.1016/j.yexcr.2004.04.036. ...

https://en.wikipedia.org/wiki/Frizzled-6

... such as p120-catenin, beta-catenin and alpha-catenin. Catenins, in turn, bind to the actin cytoskeleton. Binding of these ... Although p120 catenin is a potential substrate of PTPmu, others have suggested that the interaction between PTPmu and catenins ... In addition to catenins and cadherins, PTPmu dephosphorylates PIPKIγ90 and nectin-3 (PVRL3) to stabilize E-cadherin-based ... IQGAP1 is a scaffold for Rho family of GTPases, E-cadherin, beta-catenin and other proteins. IQGAP1 binding to Rho GTPases is ...

https://en.wikipedia.org/wiki/PTPRM

The flamingo subfamily consists of nonclassic-type cadherins; a subpopulation that does not interact with catenins. The ...

https://en.wikipedia.org/wiki/CELSR2

The flamingo subfamily consists of nonclassic-type cadherins; a subpopulation that does not interact with catenins. The ...

https://en.wikipedia.org/wiki/CELSR3

In fact, local synaptic activity changes impact the expression of the cadherin-catenin complexes. An increase in activity at a ... That is, the inter-spine competition for β-catenin determines whether a spine will be matured (increased number of complexes) ... Recent work has implicated the cadherin-catenin complex in a number of different central nervous system processes such as ... This hypothesis suggests that relative levels of cadherin-catenin complexes, which are distributed amongst spines in a local ...

https://en.wikipedia.org/wiki/Synaptic_stabilization

The flamingo subfamily consists of nonclassic-type cadherins; a subpopulation that does not interact with catenins. The ...

https://en.wikipedia.org/wiki/CELSR1

The researchers hypothesized that APC's many β-catenin binding site increase the protein's efficiency at destroying β-catenin, ... Beta-catenin,β-catenin) Human APC genome location and APC gene details page in the UCSC Genome Browser. (All articles with dead ... interacts with beta-catenin, GSK-3beta and APC and reduces the beta-catenin level". Genes to Cells. 3 (6): 395-403. doi:10.1046 ... interacts with beta-catenin, GSK-3beta and APC and reduces the beta-catenin level". Genes to Cells. 3 (6): 395-403. doi:10.1046 ...

https://en.wikipedia.org/wiki/Adenomatous_polyposis_coli

They also showed how APC functions - through binding to beta-catenin and stimulating its degradation. Vogelstein and Kinzler ... Su LK, Vogelstein B, Kinzler KW (December 1993). "Association of the APC tumor suppressor protein with catenins". Science. 262 ...

https://en.wikipedia.org/wiki/Bert_Vogelstein

"Identification of the domain of alpha-catenin involved in its association with beta-catenin and plakoglobin (gamma-catenin)". ... Plakoglobin is a member of the catenin protein family and homologous to β-catenin. Plakoglobin is a cytoplasmic component of ... Hearts also exhibited increases in β-catenin signaling. Further investigations on the role of β-catenin and plakoglobin in the ... "A mutation in alpha-catenin disrupts adhesion in clone A cells without perturbing its actin and beta-catenin binding activity ...

https://en.wikipedia.org/wiki/Plakoglobin

Clevers H, Nusse R (June 2012). "Wnt/β-catenin signaling and disease". Cell. 149 (6): 1192-205. doi:10.1016/j.cell.2012.05.012 ... Elevated levels of beta-catenin in both primary and metastases of malignant melanoma have been correlated to improved survival ... Dihlmann, Susanne; von Knebel Doeberitz, Magnus (2005-02-10). "Wnt/β-catenin-pathway as a molecular target for future anti- ... Anagnostou SH, Shepherd PR (December 2008). "Glucose induces an autocrine activation of the Wnt/beta-catenin pathway in ...

https://en.wikipedia.org/wiki/Protein_Wnt-5a

Beta-catenin is phosphorylated and degraded. An important protein in the cell cycle, Cyclin D, is inhibited by inactivated GSK- ...

https://en.wikipedia.org/wiki/CCL5

Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin ... and ZBTB33 Delta catenin Catenin CTNND2 GRCh38: Ensembl release 89: ENSG00000198561 - Ensembl, May 2017 GRCm38: Ensembl release ... p120, and called catenin delta-1 is a protein that in humans is encoded by the CTNND1 gene. This gene encodes a member of the ... "Entrez Gene: CTNND1 catenin (cadherin-associated protein), delta 1". Schackmann, R. C. J.; Tenhagen, M.; van de Ven, R. A. H.; ...

https://en.wikipedia.org/wiki/P120_(protein)

Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin ... Beta-catenin can also bind to alpha-catenin. Alpha-catenin participates in regulation of actin-containing cytoskeletal ... Loss of E-cadherin expression results in releasing β-catenin into the cytoplasm. Liberated β-catenin molecules may migrate into ... "A truncated beta-catenin disrupts the interaction between E-cadherin and alpha-catenin: a cause of loss of intercellular ...

https://en.wikipedia.org/wiki/Cadherin-1

The Pitx2 gene plays a role in lung adenocarcinoma that is dependent on activating the Wnt/β-catenin signaling pathway. When ... "Identification of a Wnt/Dvl/beta-Catenin --> Pitx2 pathway mediating cell-type-specific proliferation during development". Cell ... analyzing experimental findings from this Wnt/β-catenin signaling pathway, a TCGA dataset showed that Pitx2 had a positive ...

https://en.wikipedia.org/wiki/PITX2

Beta-catenin-like protein 1 is a protein that in humans is encoded by the CTNNBL1 gene. The protein encoded by this gene ... "Entrez Gene: CTNNBL1 catenin, beta like 1". Human CTNNBL1 genome location and CTNNBL1 gene details page in the UCSC Genome ... In addition, the encoded protein contains Armadillo/beta-catenin-like repeats, which have been implicated in protein-protein ...

https://en.wikipedia.org/wiki/CTNNBL1

Yang F, Li X, Sharma M, Sasaki CY, Longo DL, Lim B, Sun Z (March 2002). "Linking beta-catenin to androgen-signaling pathway". ... Masiello D, Chen SY, Xu Y, Verhoeven MC, Choi E, Hollenberg AN, Balk SP (October 2004). "Recruitment of beta-catenin by wild- ... Amir AL, Barua M, McKnight NC, Cheng S, Yuan X, Balk SP (August 2003). "A direct beta-catenin-independent interaction between ... Androgen receptor has been shown to interact with: AKT1, BAG1, Beta-catenin, BRCA1, C-jun, Calmodulin 1, Caveolin 1, CDK9, ...

https://en.wikipedia.org/wiki/Androgen_receptor

... ... Abstract Developmentally Programmed Tankyrase Activity Upregulates Beta-catenin and Licenses Progression of Embryonic Genome ... Story Developmentally Programmed Tankyrase Activity Upregulates Beta-catenin and Licenses Progression of Embryonic Genome ... Activation] [Synopsis Developmentally Programmed Tankyrase Activity Upregulates Beta-catenin and Licenses Progression of ...

https://www.niehs.nih.gov/research/resources/articles_journals/recent/pdf_2020/developmentally_programmed_tankyrase_activity_upregulates_betacatenin_and_licenses_progression_of_embryonic_genome_activation.cfm

... Nat Commun. 2020 Jun 12;11(1):2984. doi: 10.1038/ ... Loss of ADNP promotes the formation of the degradation complex and β-Catenin degradation via ubiquitin-proteasome pathway, ... Mechanistically, ADNP functions to stabilize β-Catenin through binding to its armadillo domain which prevents its association ...

https://pubmed.ncbi.nlm.nih.gov/32533114/

Importantly, we showed that EGFR-phosphorylated PFKP Y64 has a critical role in AKT activation and AKT-mediated β-catenin S552 ... Importantly, we showed that EGFR-phosphorylated PFKP Y64 has a critical role in AKT activation and AKT-mediated β-catenin S552 ... These findings highlight a novel mechanism underlying a glycolytic enzyme-mediated β-catenin activation and underscore the ... These findings highlight a novel mechanism underlying a glycolytic enzyme-mediated β-catenin transactivation and underscore the ...

https://www.frontiersin.org/articles/10.3389/fonc.2020.00211/full

Michael Davidsons lab contains the insert Beta-Catenin. This plasmid is available through Addgene. ... mCerulean-Beta-Catenin-20 was a gift from Michael Davidson (Addgene plasmid # 55352 ; http://n2t.net/addgene:55352 ; RRID: ...

https://www.addgene.org/55352/

Overexpression of δ-catenin increased the tumor invasion in vitro and in vivo However, δ-catenin knockdown decreased glioma ... δ-Catenin Promotes Bevacizumab-Induced Glioma Invasion Toshihiko Shimizu 1 , Joji Ishida 1 , Kazuhiko Kurozumi 2 , Tomotsugu ... δ-Catenin Promotes Bevacizumab-Induced Glioma Invasion Toshihiko Shimizu et al. Mol Cancer Ther. 2019 Apr. ... Delta-catenin attenuates medulloblastoma cell invasion by targeting EMT pathway. Hu Y, Zhu S, Xu R, Wang M, Chen F, Zhang Z, ...

https://pubmed.ncbi.nlm.nih.gov/30872378/

The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. ...

http://www.ncbi.nlm.nih.gov/protein/XP_001065606

More info for Superfamily a.24.9: alpha-catenin/vinculin-like. Timeline for Superfamily a.24.9: alpha-catenin/vinculin-like: * ... Superfamily a.24.9: alpha-catenin/vinculin-like appears in SCOPe 2.06. *Superfamily a.24.9: alpha-catenin/vinculin-like appears ... a.24.9.1: alpha-catenin/vinculin [47221] (3 proteins). possible duplication: contains several domains of this fold. The listed ... Lineage for Superfamily a.24.9: alpha-catenin/vinculin-like. *Root: SCOPe 2.07 *. Class a: All alpha proteins [46456] (289 ...

http://scop.berkeley.edu/sunid=47220&ver=2.07

Proteintech Anti-beta-Catenin Polyclonal, Catalog # 51067-2-AP. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), ...

https://www.thermofisher.com/antibody/product/beta-Catenin-Antibody-Polyclonal/51067-2-AP

We found that N6L and nucleolin interact with β-catenin. We found that the Wnt/β-catenin pathway is activated in PDAC and is ... In conclusion, in this study, we identified β-catenin as a new nucleolin interactor and suggest that the Wnt/β-catenin pathway ... N6L also inhibited the growth and the activation of the Wnt/β-catenin pathway in vivo in mice and in 3D cultures derived from ... N6L and nucleolin loss of function induced by siRNA inhibited Wnt pathway activation by preventing β-catenin stabilization in ...

https://www.mdpi.com/2072-6694/13/12/2986

Therapeutic Targeting of Wnt/β-Catenin Signaling to Improve Bone Formation in MPS VII. July 21, 2017. Skeletal disease is ... Wnt/B-catenin signaling is a crucial positive regulator of bone formation and our preliminary data suggests impaired activation ... Our overall objective in this proposal is to investigate the role of Wnt/B-catenin signaling dysregulation in the etiology of ... Therapeutic Targeting of Wnt/β-Catenin Signaling to Improve Bone Formation in MPS VII ...

https://mpssociety.org/therapeutic-targeting-wnt%CE%B2-catenin-signaling-improve-bone-formation-mps-vii/

Mechanistically, HEG1 promotes β-catenin expression and maintains its stability, leading to intracellular β-catenin ... HEG1, hepatocellular carcinoma, prognosis, metastasis, epithelial-mesenchymal transition (EMT), Wnt/β-catenin signaling ... Loss- and gain-of-function assays further confirmed that β-catenin is essential for HEG1-mediated promotion of HCC invasion, ... HEG1 indicates poor prognosis and promotes hepatocellular carcinoma invasion, metastasis, and EMT by activating Wnt/β-catenin ...

https://portlandpress.com/clinsci/article-abstract/133/14/1645/219062/HEG1-indicates-poor-prognosis-and-promotes?redirectedFrom=fulltext

PIC is an active protein tyrosine phosphatase that binds to and dephosphorylates {beta}-catenin, an RPTP{kappa} substrate. The ... Thus, both isoforms have acquired opposing roles in the regulation of {beta}-catenin signaling. We also found that RPTPmu, ... Furin-, ADAM 10-, and γ-secretase-mediated cleavage of a receptor tyrosine phosphatase and regulation of β-catenins ... expression of RPTP{kappa} suppresses {beta}-catenins transcriptional activity, whereas the expression of PIC increases it. ...

https://www.mdc-berlin.de/research/publications/furin-adam-10-and-g-secretase-mediated-cleavage-receptor-tyrosine-phosphatase

Compared with wild-type beta-catenin (WT), the beta-catenin mutants D32G, D32N, and D32Y strongly activated TCF-4-dependent ... revealed ubiquitinated bands on all three mutant beta-catenins, as well as on WT beta-catenin. The relative order of ... the phosphorylation of Ser33 appeared to be affected in the beta-catenin mutants. Specifically, antiphospho-beta-catenin 33/37/ ... Beta-Catenin, a member of the Wnt signaling pathway, is downregulated by glycogen synthase kinase-3beta (GSK-3beta)-dependent ...

https://lpi.oregonstate.edu/biblio/phosphorylation-and-ubiquitination-oncogenic-mutants-beta-catenin-containing-substitutions

A-C) β-catenin-positive and (D-F) β-catenin-negative NSCLC. Staining for (A and D) β-catenin, (B and E) CD8, and (C and F) ... in the β-catenin-negative group. NSCLC-expressing β-catenin had a significantly higher TMB than non-β-catenin-expressing lung ... catenin cases. Similarly, CD11c+ cell infiltration was significantly lower in the β‑catenin‑positive group. The β‑catenin‑ ... Impact of β-catenin on prognosis. In β-catenin-positive NSCLC, (A) the OS was predominantly worse and (B) the RFS tended to be ...

https://www.spandidos-publications.com/10.3892/ol.2021.12464?text=fulltext

Kaiso Interacts with p120-Catenin to Regulate β-Catenin Expression at the Transcriptional Level ... whereas high Kaiso expression significantly down-regulated β-catenin mRNA expression (P = 0.004). The expression of β-catenin ... P = 0.065 for 24 h, P = 0.007 for 48 h; P = 0.009 for 96 h, respectively). Analysis of β-catenin mRNA expression in SPC ... geneticsMolecular geneticsGene regulationMolecular cell biologySignal transductionSignaling in cellular processesBeta-catenin ...

https://figshare.com/articles/figure/_Kaiso_suppresses_946_catenin_mRNA_expression_in_cell_lines_that_have_not_been_treated_with_5_Aza_CdR_/926124/1

Background: Vascular smooth muscle cells (VSMCs) undergo a conversion from a contractile phenotype to a proliferative synthetic phenotype, contributing to the pathogenesis of cardiovascular diseases. Semaphorin 7A (SEMA7A) is... | Find, read and cite all the research you need on Tech Science Press

https://www.techscience.com/biocell/v47n4/51790/pdf

Wnt signaling drives WRM-1/beta-catenin asymmetries in early C. elegans embryos. Genes Dev. 2005 Aug 01; 19(15):1749-54. ... Wnt signaling drives WRM-1/beta-catenin asymmetries in early C. elegans embryos. ... Wnt signaling drives WRM-1/beta-catenin asymmetries in early C. elegans embryos. ...

https://profiles.umassmed.edu/display/69174

Moreover, anti-β-catenin IP was employed to investigate the decrease of total β-catenin in the scenario (Figure 4D). ... with anti-β-catenin IP (D). GAPDH and EGFR were used as a loading control. (E) Measurement of β-catenin ratio in membrane- ... β-catenin has dual roles in the regulation of cancer progression. On one hand, β-catenin binds to ECAD in membrane where it ... Total β-catenin is explained by its subcellular accumulation. Immunocytochemistry staining shows that accumulation of β-catenin ...

https://www.oncotarget.com/article/15910/text/

Colon cancer (CC) is the third most common cancer worldwide. Emodin is an anthraquinone-active substance that has the ability to affect tumor progression. Our study aims to explore the effects and the relevant mechanism of ... | Find, read and cite all the research you need on Tech Science Press

https://techscience.com/or/v27n2/48644/pdf

The Wnt & β-Catenin Targeted Drug Discovery Summit is the only meeting uniting leading pharma, pioneering biotechs and reputed ... academics under a mutual and ambitious objective of accelerating your novel Wnt/β-catenin candidates into clinic and market. ... Wnt & B-Catenin Targeted Drug Discovery Summit 2021. 11/17/2021 - 11/18/2021. ...

https://pharmaleaders.com/event/wnt-b-catenin-targeted-drug-discovery-summit-2021/

S6C). In addition, tumors showed high levels of nuclear β-catenin, consistent with the activation of β-catenin owing to Cre- ... β-cateninexon3/+K-RasG12D/+ cohort compared with the UroIICre+Fgfr3+/K644Eβ-cateninexon3/+ mice (n=3, P,0.001; supplementary ... β-cateninexon3/+. In comparison, lung tumors in UroIICre+β-cateninexon3/+K-RasG12D/+ model show minimal upregulation of pAKT (I ... β-catenin activating mutations (Harada et al., 1999) in UroIICre+Fgfr3+/K644Eβ-cateninexon3/+ mice (n=27) (supplementary ...

https://journals.biologists.com/dmm/article/4/4/548/2566/K-Ras-and-catenin-mutations-cooperate-with-Fgfr3

Levels of PKA (protein kinase A)-phosphorylated β-catenin S675 and β-catenin association with PKA, BIG1, and BIG2 were also ... Enhancement of β-catenin activity by BIG1 plus BIG2 via Arf activation and cAMP signals. Thursday, September 15, 2016. - Poster ... Multifunctional β-catenin, with critical roles in both cell-cell adhesion and Wnt-signaling pathways, was among HeLa cell ... Direct interaction of BIG1 N-terminal sequence with β-catenin, found in yeast two-hybrid studies, was confirmed using in vitro- ...

https://researchfestival.nih.gov/2016/posters/enhancement-b-catenin-activity-big1-plus-big2-arf-activation-and-camp

"Why throw β-catenin overboard instead of simply grinding it up? One possibility is that exosomal β-catenin is like a message in ... exosomes may be a more reversible way for cells to lower their β-catenin levels. "Incorporating β-catenin into exosomes could ... Beta-catenin binds E-cadherin to facilitate the formation of intercellular adhesions, but its also a central component of the ... β-Catenin could be targeted to these vesicles through the interaction of E-cadherin with tetraspanins. E-cadherin was also ...

https://rupress.org/jcb/article/190/6/945/36070/Catenin-gets-an-honorable-discharge

Here we show that p120 catenin ARM domains 1, 3-5, and 8 mediate interactions between p120 catenin and MUC1, and that these ... The mechanisms by which MUC1 and p120 catenin contribute to progression of cancers from early transformation to metastasis are ... We also show that different isoforms of p120 catenin, when coexpressed with MUC1, create cells that exhibit distinct patterns ... Interactions between MUC1 and p120 catenin regulate dynamic features of cell adhesion, motility, and metastasis.. ...

https://edrn.nci.nih.gov/data-and-resources/publications/24371222-2299-interactions-between-muc1-and-p120-catenin-regulate-dynamic-features-of-cell-adhesion-motility-and-metastasis/

β-Catenin and p110δinhibition in vitro augments donor Th17 tumor immunity in vivo. β-Catenin and p110δ inhibition reduces IL- ... P110δandβ-catenin inhibitors expand memory from differentiated Th17 cultures. Finally, we determined if p110δ and β-catenin ... P110δandβ-catenin inhibition propagate precursor Th17 cells. We next asked how p110δ and β-catenin inhibition modulate Th17 ... Wnt/β-catenin and cytokines rebound in dual-inhibited antitumor Th17 cells. (A) β-Catenin accumulates in the nuclei of ICOS- ...

https://insight.jci.org/articles/view/90547

β-Catenin expression and allelic loss at APC in sporadic colorectal carcinogenesis Share Share Share ... β-Catenin expression and allelic loss at APC in sporadic colorectal carcinogenesis ...

https://www.ndm.ox.ac.uk/publications/72201

β-catenin mutations in craniopharyngiomas and pituitary adenomas. Eftychia Oikonomou, Diele C. Barreto, Beatriz Soares, Luiz de ... Dive into the research topics of β-catenin mutations in craniopharyngiomas and pituitary adenomas. Together they form a ...

https://research.aston.ac.uk/en/publications/%CE%B2-catenin-mutations-in-craniopharyngiomas-and-pituitary-adenomas/fingerprints/

... catenin complex formation. Yap1 and -catenin activities were reduced in the Piezo1 and Piezo1/2 mutant bones and such defects ... Piezo1/2 mediate mechanotransduction essential for bone formation through concerted activation of NFAT-YAP1-catenin Zhou, T;Gao ... catenin transcription factors by inducing their dephosphorylation as well as NFAT/YAP1/- ...

https://listlabs.com/citations_products/piezo1-2-mediate-mechanotransduction-essential-for-bone-formation-through-concerted-activation-of-nfat-yap1-catenin/

Wnt/β-catenin Pathway Functions by Inactivation of Tcf7l1 Protein ... The canonical Wnt/β-catenin signaling pathway classically functions through the activation of target genes by Tcf/Lef-β-catenin ... T-cell factor (Tcf3)T-cell factor 7l1 (Tcf7l1)Wnt signalingMouse embryogenesisbeta-cateninEmbryonic stem cells ... Thus, β-catenin binding to Tcf7l1 is necessary and sufficient for destabilizing Tcf7l1 protein in stem cells. We ablated the ...

https://indigo.uic.edu/articles/thesis/Wnt_-catenin_Pathway_Functions_by_Inactivation_of_Tcf7l1_Protein/10820615/1

Sophoridine depends on targeting ESRRG/β-catenin pathway to exert tumor-suppressive activities in gastric cancer cells and ... Molecular studies further revealed that Sophoridine promoted β-catenin degradation by enhancing Estrogen-related receptor gamma ... From: Sophoridine exerts tumor-suppressive activities via promoting ESRRG-mediated β-catenin degradation in gastric cancer ...

https://bmccancer.biomedcentral.com/articles/10.1186/s12885-020-07067-x/peer-review

Wnt/B-catenin signaling is a crucial positive regulator of bone formation and our preliminary data suggests impaired activation of this pathway coincident with the earliest manifestations of bone disease in MPS VII dogs. (mpssociety.org)
Our overall objective in this proposal is to investigate the role of Wnt/B-catenin signaling dysregulation in the etiology of failed vertebral bone formation in MPS VII and establish this pathway as a viable therapeutic target. (mpssociety.org)
The relationship between long non-coding RNAs and Wnt/β-catenin signaling pathway in the pathogenesis of Alzheimer's disease. (nih.gov)
Overloaded axial stress activates the Wnt/β-Catenin pathway in nucleus pulposus cells of adult degenerative scoliosis combined with intervertebral disc degeneration. (nih.gov)
Beta-catenin binds E-cadherin to facilitate the formation of intercellular adhesions, but it's also a central component of the Wnt signaling pathway that controls cell proliferation and differentiation ( 1 ). (rupress.org)
Activation of the Wnt pathway stabilizes β-catenin, enabling it to translocate into the nucleus and pair up with transcription factors to induce target gene expression. (rupress.org)
found that CD9 and CD82 boosted the release of exosomes containing β-catenin, thereby reducing cellular levels of the protein and inhibiting the Wnt pathway. (rupress.org)
The canonical Wnt/β-catenin signaling pathway classically functions through the activation of target genes by Tcf/Lef-β-catenin complexes. (uic.edu)
I determined that mouse Tcf7l1 (formerly named Tcf3) mediates a distinct and new form of regulation of the pathway, which is independent of transactivator activity of Tcf7l1-β-catenin complexes. (uic.edu)
The pathway is regulated by several antagonists that inhibit canonical and/or non-canonical Wnt/β-catenin signaling. (biomedcentral.com)
Liao S , Chen H , Liu M , Gan L , Li C , Zhang W , Lv L , Mei Z , . Aquaporin 9 inhibits growth and metastasis of hepatocellular carcinoma cells via Wnt/β-catenin pathway. (aging-us.com)
Taken together, our results suggested that overexpressed AQP9 could inhibit growth and metastasis of HCC cells via Wnt/β-catenin pathway. (aging-us.com)
Therefore, we investigated the effect of LINC01314 on the development of GC cells in relation to the Wnt/β-catenin signaling pathway. (biomedcentral.com)
The findings indicated that over-expression of LINC01314 down-regulated KLK4 to inhibit the activation of the Wnt/β-catenin signaling pathway, thus suppressing migration, invasion, and angiogenesis in GC cells, which provides new insight for the treatment of GC. (biomedcentral.com)
Genistein ameliorated experimentally induced gastric ulcer in rats via inhibiting gastric tissues fibrosis by modulating Wnt/ß-catenin/TGF-ß/PKB pathway. (bvsalud.org)
Therefore, we aimed to investigate the ability of genistein to reduce experimentally induced GU in rats by affecting gastric tissue fibrosis Wnt/ß- catenin /TGF-ß/SMAD4 pathway. (bvsalud.org)
Our results showed that oral administration of ICS II could alleviate cognitive injuries of APP/PS1 mice, promote hippocampal neurogenesis, as well as stimulate Wnt/β-catenin signal pathway confirmed by upregulated Wnt-3a, phosphorylated glycogen synthase kinase-3β (p-GSK-3β), and β-catenin. (nih.gov)
However, these effects of ICS II were blunted by XAV-939, an inhibitor of Wnt/β-catenin signaling pathway. (nih.gov)
In summary, our findings revealed that ICS II could improve neurogenesis and inhibit mitochondrial fission via activation of the Wnt/β-catenin signaling pathway, which contributed to cognitive function restoration of APP/PS1 mice. (nih.gov)
Cbfβ inhibits fat cell formation through an important cell signaling pathway called Wnt/β-catenin. (nih.gov)

We previously showed that EGFR-induced β-catenin transactivation is regulated by mechanisms distinct from Wnt-dependent canonical signaling ( 12 - 14 ). (frontiersin.org)
Extracellular Vesicles Released from Cancer Cells Promote Tumorigenesis by Inducing Epithelial to Mesenchymal Transition via β-Catenin Signaling. (nih.gov)
Mechanistically, HEG1 promotes β-catenin expression and maintains its stability, leading to intracellular β-catenin accumulation, β-catenin nuclear translocation and Wnt signaling activation. (portlandpress.com)
Thus, both isoforms have acquired opposing roles in the regulation of {beta}-catenin signaling. (mdc-berlin.de)
Our results identify intramembrane proteolysis as a regulatory switch in RPTP{kappa} signaling and implicate PIC in the activation of {beta}-catenin-mediated transcription. (mdc-berlin.de)
Wnt signaling drives WRM-1/beta-catenin asymmetries in early C. elegans embryos. (umassmed.edu)
Overexpressing CD82 or a related tetraspanin called CD9 suppressed Wnt signaling and reduced β-catenin protein levels. (rupress.org)
The exosomal release of β-catenin may be compromised in certain cancers, where Wnt signaling is often hyperactive. (rupress.org)
CD82 may act as a metastasis suppressor by targeting β-catenin for exosomal release and thereby reducing its availability as a Wnt signaling mediator," Caplan proposes. (rupress.org)
Multifunctional β-catenin, with critical roles in both cell-cell adhesion and Wnt-signaling pathways, was among HeLa cell proteins co-immunoprecipitated by antibodies against BIG1 or BIG2. (nih.gov)
Mouse Anti β Catenin, supplied by Cell Signaling Technology Inc, used in various techniques. (bioz.com)
Nm 006555 Sirna Targeting B Catenin Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. (bioz.com)
In vivo studies indicated that tumor volume and weight were remarkably decreased in AQP9 overexpression group, where the levels of Wnt/β-catenin signaling- and EMT-associated molecules were also reduced. (aging-us.com)
Cbfβ governs osteoblast-adipocyte lineage commitment through enhancing β-catenin signaling and suppressing adipogenesis gene expression. (nih.gov)

A , model based upon the assumption that α -catenin binds simultaneously to β -catenin and actin filaments. (nih.gov)
PIC is an active protein tyrosine phosphatase that binds to and dephosphorylates {beta}-catenin, an RPTP{kappa} substrate. (mdc-berlin.de)
β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. (phosphosolutions.com)

Increased transcriptional activity of β-catenin, which is essential for cell proliferation, migration, invasion, and survival ( 1 , 2 ), has been detected in many types of human cancer ( 3 - 6 ). (frontiersin.org)
Each Tcf/Lef in mammals is believed to function as a switch, going from a transcriptional repressor in the absence of Wnt stabilized β-catenin to a transcriptional activator in the presence of Wnt-stabilized β-catenin. (uic.edu)
In addition, transcriptional activity of β-catenin was lower in HeLa cells after BIG1 and/or BIG2 depletion, further consistent with their roles in regulating β-catenin action via S675 phosphorylation. (nih.gov)
The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. (phosphosolutions.com)

Interactions between MUC1 and p120 catenin regulate dynamic features of cell adhesion, motility, and metastasis. (nih.gov)
The mechanisms by which MUC1 and p120 catenin contribute to progression of cancers from early transformation to metastasis are poorly understood. (nih.gov)
Here we show that p120 catenin ARM domains 1, 3-5, and 8 mediate interactions between p120 catenin and MUC1, and that these interactions modulate dynamic properties of cell adhesion, motility, and metastasis of pancreatic cancer cells. (nih.gov)
We also show that different isoforms of p120 catenin, when coexpressed with MUC1, create cells that exhibit distinct patterns of motility in culture (motility independent of cell adhesion, motility within a monolayer while exchanging contacts with other cells, and unified motility while maintaining static epithelial contacts) and patterns of metastasis. (nih.gov)
In addition, both RhoA and p190RhoGAP-B colocalize with p120-catenin at sites of cell-cell contact. (nih.gov)
The association between p190RhoGAP-B and p120-catenin is regulated by matrix compliance such that it increases in compliant vs. rigid collagen gels. (nih.gov)
Furthermore, knockdown of p120-catenin disrupts ductal morphogenesis, disregulates RhoA activity, and results in loss of p190B at cell-cell contacts. (nih.gov)
Overall, these results demonstrate that RhoA is down-regulated at cell-cell contacts through p190RhoGAP-B, which is localized to cell-cell contacts by association with p120-catenin that is regulated by tensional homeostasis. (nih.gov)

CTNNA3 (alpha-catenin) gene variants are associated with diisocyanate asthma: a replication study in a Caucasian worker population. (cdc.gov)

B , model based upon data from Refs and that α -catenin monomers and dimers regulate binding to β -catenin and actin filaments, respectively, and that α -catenin also inhibits Arp2/3-mediated actin branching and promotes actin bundling. (nih.gov)
Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. (phosphosolutions.com)
Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions leading to changes in cell adhesion. (phosphosolutions.com)

Characterization of the Subcellular Distribution of Phospho-β-catenin in Colorectal Cancer. (nih.gov)
The discovery of genes responsible for inherited colorectal cancer syndromes has been important in identifying important etiologic pathways such as the beta-catenin/ APC and TGF beta/SMAD pathways. (cdc.gov)

Importantly, we showed that EGFR-phosphorylated PFKP Y64 has a critical role in AKT activation and AKT-mediated β-catenin S552 phosphorylation and subsequent β-catenin transactivation and promotion of tumor cell glycolysis, migration, invasion, proliferation, and brain tumor growth. (frontiersin.org)
EGFR activation-induced and CK2α-mediated α-catenin phosphorylation releases β-catenin from the β-catenin/α-catenin protein complex whereas nuclear pyruvate kinase M2 (PKM2) associates with β-catenin and induces gene expression by direct phosphorylation of histone H3 ( 12 - 17 ). (frontiersin.org)
The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. (phosphosolutions.com)

Loss- and gain-of-function assays further confirmed that β-catenin is essential for HEG1-mediated promotion of HCC invasion, metastasis and EMT. (portlandpress.com)
reveal a new way in which cells restrain β-catenin and potentially suppress tumor metastasis: the protein can be ejected from cells in small vesicles called exosomes ( 2 ). (rupress.org)
CD82 may act as a metastasis suppressor by targeting β-catenin for exosomal release. (rupress.org)

Here, we demonstrate that phosphofructokinase 1 platelet isoform (PFKP), which catalyzes a rate-limiting reaction in glycolysis, promotes EGFR activation-induced nuclear translocation and activation of β-catenin, thereby enhancing the expression of its downstream genes CCND1 and MYC in human glioblastoma cells. (frontiersin.org)
In addition, AKT directly phosphorylates β-catenin at Ser552 (S552), which promotes nuclear translocation and transactivation of β-catenin ( 18 ). (frontiersin.org)
β-catenin promotes resistance to trastuzumab in breast cancer cells through enhancing interaction between HER2 and SRC. (nih.gov)

Both phospholipase D activity and vesicular trafficking were required for effects of BIG1 and BIG2 on β-catenin activation. (nih.gov)
31. Beta-catenin nuclear labeling is a common feature of sessile serrated adenomas and correlates with early neoplastic progression after BRAF activation. (nih.gov)

β-catenin transactivation leads to enhanced T-cell factor (TCF)/lymphoid enhancer factor (LEF)-driven transcription of genes, such as CCND1 (encoding cyclin D1) and MYC (encoding c-Myc) ( 7 - 9 ). (frontiersin.org)
Instead of switching Tcf7l1 into a transactivator, Wnt/β-catenin reduces Tcf7l1 protein levels, which relieves repression of target genes, such as Lef1. (uic.edu)

These findings highlight a novel mechanism underlying a glycolytic enzyme-mediated β-catenin transactivation and underscore the integrated and reciprocal regulation of metabolism and gene expression, which are two fundamental biological processes in tumor development. (frontiersin.org)
β‑catenin expression by tumor cells suppressed dendritic cell recruitment to the tumor microenvironment in a melanoma model, resulting in fewer tumor‑infiltrating lymphocytes. (spandidos-publications.com)
Immunohistochemistry was used in the present study to examine the association between the expression of β‑catenin and tumor infiltrating lymphocytes and CD11c + cells in 122 patients with non‑small cell lung cancer (NSCLC), who underwent radical surgery. (spandidos-publications.com)
There was no significant association between the expression of β‑catenin and the frequency of CD8 + cell infiltration into tumor tissues, including the stroma. (spandidos-publications.com)
Furthermore, β‑catenin‑positive NSCLC had a high tumor mutation burden, but tended to have a low expression of programmed death‑ligand 1. (spandidos-publications.com)
In conclusion, the expression of β‑catenin in NSCLC was negatively associated with CD11c + cells and cytotoxic T cell infiltration at the tumor site and had a tendency towards a poor prognosis. (spandidos-publications.com)
Kaiso suppresses β-catenin mRNA expression in cell lines that have not been treated with 5-Aza-CdR. (figshare.com)
Analysis of β-catenin mRNA expression in SPC following treatment of using RT-PCR (C) and Real-Time PCR by ANOVA (D) showed that treatment with 5-Aza-CdR demethylation reagent (7 µmol/L) for 48 h resulted in significant upregulation ( P = 0.007), whereas high Kaiso expression significantly down-regulated β-catenin mRNA expression ( P = 0.004). (figshare.com)
The expression of β-catenin mRNA expression in cells treated with 5-Aza-CdR reagent did not change significantly in the presence of high Kaiso expression ( P = 0.062). (figshare.com)
We ablated the Tcf7l1-β-catenin interaction by generating a mouse Tcf7l1ΔN knockin allele and show Tcf7l1ΔN blocks Wnt-reduction of Tcf7l1, inhibits Lef1 expression, and causes peri-natal lethality in Tcf7l1ΔN/ΔN mice. (uic.edu)
Depletion of BIG1 and/or BIG2 or over-expression of GEF-inactive mutant, but not wild type, BIG1 or BIG2 interfered with β-catenin trafficking, leading to accumulation at perinuclear Golgi structures. (nih.gov)
Association between clinicopathological factors and the expression of β-catenin. (bioz.com)
Expression of β-catenin in BC tissues and cell lines. (bioz.com)
The expression of β-catenin was determined in 32 paired BC tissues at the (A) mRNA and (B) protein levels were determined by RT-qPCR and western blot analysis, respectively. (bioz.com)
C) The expression of β-catenin was analyzed in BC tissues by immunohistochemistry. (bioz.com)
The expression levels of β-catenin in the BC MCF-10A, MDA-MB-468 and T47D cell lines and MCF-7 cells at the (E) mRNA and (F and G) protein levels were determined by RT-qPCR and western blot analysis, respectively. (bioz.com)
In addition, treatment with genistein significantly reduced the expression of Wnt, ß- catenin , TGF-ß, SMAD4, and PKB. (bvsalud.org)
Besides antioxidant activity , genistein improves experimentally induced GU in rats , at least in part, via reduction of gastric tissue fibrosis as indicated by reduction in expression of Wnt, ß- catenin , TGF-ß, SMAD4, and PKB. (bvsalud.org)

Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. (phosphosolutions.com)

Conversely, the infiltration of CD8 + cells into tumor nests was significantly lower in β‑catenin‑positive cases compared with that in negative β‑catenin cases. (spandidos-publications.com)
These findings impact understanding of the mechanisms whereby Wnt/β-catenin mediates its effects, especially in Tcf7l1-expressing stem cells and tumor cells. (uic.edu)

The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. (phosphosolutions.com)

β-Catenin could be targeted to these vesicles through the interaction of E-cadherin with tetraspanins. (rupress.org)
Interestingly, Tcf7l1-/ΔN mice developed into viable and ostensibly normal adult mice, demonstrating that reducing the amount of Tcf7l1 replaced the requirement for Tcf7l1-β-catenin interaction. (uic.edu)
Direct interaction of BIG1 N-terminal sequence with β-catenin, found in yeast two-hybrid studies, was confirmed using in vitro-synthesized proteins. (nih.gov)

Experiments using mouse embryonic stem cells show that recombinant Wnt3a, GSK3-inhibition, or simply increasing β-catenin levels were all sufficient to reduce Tcf7l1 protein independent of mRNA levels. (uic.edu)

Structural and functional characterization of Caenorhabditis elegans α-catenin reveals constitutive binding to β-catenin and F-actin. (nih.gov)

The gastric tissues were isolated to investigate markers of gastric fibrosis , Wnt, ß- catenin , transforming growth factor (TGF)-ß, SMAD4, and Protein kinase B (PKB). (bvsalud.org)

The levels of KLK4, Wnt-1, β-catenin, cyclin D1, N-cadherin and E-cadherin were measured, and cell invasion and migration were evaluated. (biomedcentral.com)
Overexpressed LINC01314 or silencing of KLK4 led to inhibited GC cell migration and invasion, corresponding to decreased Wnt-1, β-catenin, cyclin D1 and N-cadherin while increased E-cadherin. (biomedcentral.com)

This human sequence is highly conserved in rat and mouse β-Catenins. (phosphosolutions.com)

The effect did not occur in Tcf7l1ΔN/ΔN ES cells, which lack nine Tcf7l1 residues necessary for binding to β-catenin. (uic.edu)
β-Catenin synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human β-Catenin. (phosphosolutions.com)

Israely I, Costa RM, Xie CW, Silva AJ, Kosik KS, Liu X. Deletion of the neuron-specific protein delta-catenin leads to severe cognitive and synaptic dysfunction. (medlineplus.gov)

Similarly, CD11c + cell infiltration was significantly lower in the β‑catenin‑positive group. (spandidos-publications.com)

Alternatively, exosomes may be a more reversible way for cells to lower their β-catenin levels. (rupress.org)
Levels of PKA (protein kinase A)-phosphorylated β-catenin S675 and β-catenin association with PKA, BIG1, and BIG2 were also diminished after BIG1/BIG2 depletion. (nih.gov)
The levels of PCNA, E-cad, N-cad, α-SMA, DVL2, GSK-3β, cyclinD1 and β-catenin in HCC cells were reduced by overexpressed AQP9, while cell apoptosis was remarkably enhanced. (aging-us.com)

Delta-catenin appears to play a crucial role in the function of synapses. (medlineplus.gov)

Caplan and colleagues now want to investigate whether this is how tetraspanins target β-catenin to exosomes. (rupress.org)

β-catenin can be activated not only by Wnt ligands but also by receptor tyrosine kinases, such as epidermal growth factor receptor (EGFR), whose mutation or overexpression of EGFR gene occurs in many types of human cancer, including more than 50% of glioblastoma (GBM) ( 10 , 11 ). (frontiersin.org)

Recently, a genome-wide association study (GWAS) conducted in Korean subjects identified four CTNNA3 (alpha-T catenin) single nucleotide polymorphisms (SNPs) (rs10762058, rs7088181, rs1786929, and rs4378283) associated with diisocyanate -induced occupational asthma (DA). (cdc.gov)

Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. (phosphosolutions.com)

Vice versa, after the cells were treated with Wnt/β-catenin inducer (SKL2001), the effects caused by overexpressed AQP9 were abrogated. (aging-us.com)

Anatomy9

Organisms1

Chemicals and Drugs16

Analytical, Diagnostic and Therapeutic Techniques and Equipment5

Phenomena and Processes7

Disciplines and Occupations1

Information Science2

Misexpression of the catenin p120(ctn)1A perturbs Xenopus gastrulation but does not elicit Wnt-directed axis specification. (1/407)

The catenin p120(ctn) interacts with Kaiso, a novel BTB/POZ domain zinc finger transcription factor. (2/407)

p120(ctn) acts as an inhibitory regulator of cadherin function in colon carcinoma cells. (3/407)

Functional characterization of multiple transactivating elements in beta-catenin, some of which interact with the TATA-binding protein in vitro. (4/407)

Nuclear localization of the p120(ctn) Armadillo-like catenin is counteracted by a nuclear export signal and by E-cadherin expression. (5/407)

p120(ctn) binds to the membrane-proximal region of the E-cadherin cytoplasmic domain and is involved in modulation of adhesion activity. (6/407)

N-Cadherin expression in human prostate carcinoma cell lines. An epithelial-mesenchymal transformation mediating adhesion withStromal cells. (7/407)

Epithelial mesenchymal transition by c-Fos estrogen receptor activation involves nuclear translocation of beta-catenin and upregulation of beta-catenin/lymphoid enhancer binding factor-1 transcriptional activity. (8/407)

Catenins

alpha Catenin

gamma Catenin

Desmoplakins

Cadherins

beta Catenin

Cytoskeletal Proteins

Adherens Junctions

Trans-Activators

Cell Adhesion Molecules

Intercellular Junctions

Cell Adhesion

Armadillo Domain Proteins

Desmogleins

Phosphoproteins

Cell Aggregation

Precipitin Tests

L Cells (Cell Line)

Cytoskeleton

Actins

Xenopus Proteins

Epithelial Cells

Protein Binding

Dogs

Cell Line

Cells, Cultured

Cell Communication

Fluorescent Antibody Technique

Transfection

Phosphorylation

Tumor Cells, Cultured

Molecular Sequence Data

Immunohistochemistry

Amino Acid Sequence

Cell Membrane

Recombinant Fusion Proteins

Antigens, CD

Blotting, Western

Misexpression of the catenin p120(ctn)1A perturbs Xenopus gastrulation but does not elicit Wnt-directed axis specification. (1/407)

The catenin p120(ctn) interacts with Kaiso, a novel BTB/POZ domain zinc finger transcription factor. (2/407)

p120(ctn) acts as an inhibitory regulator of cadherin function in colon carcinoma cells. (3/407)

Functional characterization of multiple transactivating elements in beta-catenin, some of which interact with the TATA-binding protein in vitro. (4/407)

Nuclear localization of the p120(ctn) Armadillo-like catenin is counteracted by a nuclear export signal and by E-cadherin expression. (5/407)

p120(ctn) binds to the membrane-proximal region of the E-cadherin cytoplasmic domain and is involved in modulation of adhesion activity. (6/407)

N-Cadherin expression in human prostate carcinoma cell lines. An epithelial-mesenchymal transformation mediating adhesion withStromal cells. (7/407)

Epithelial mesenchymal transition by c-Fos estrogen receptor activation involves nuclear translocation of beta-catenin and upregulation of beta-catenin/lymphoid enhancer binding factor-1 transcriptional activity. (8/407)

Catenin

Alpha catenin

Delta catenin

Catenin beta-1

Catenin alpha-1

Cadherin-catenin complex in learning and memory

Dishevelled binding antagonist of beta catenin 2

Dishevelled binding antagonist of beta catenin 1

Signaling Gateway (website)

Endometrial cancer

CTNNBIP1

Mouse model of colorectal and intestinal cancer

Vinculin family

TBX2

Frizzled-6

PTPRM

CELSR2

CELSR3

Synaptic stabilization

CELSR1

Adenomatous polyposis coli

Bert Vogelstein

Plakoglobin

Protein Wnt-5a

CCL5

P120 (protein)

Cadherin-1

PITX2

CTNNBL1

Androgen receptor

Developmentally Programmed Tankyrase Activity Upregulates Beta-catenin and Licenses Progression of Embryonic Genome Activation

ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling

Frontiers | Phosphofructokinase 1 Platelet Isoform Promotes β-Catenin Transactivation for Tumor Development

Addgene: mCerulean-Beta-Catenin-20