A family of cytoskeletal proteins that play essential roles in CELL ADHESION at ADHERENS JUNCTIONS by linking CADHERINS to the ACTIN FILAMENTS of the CYTOSKELETON.
A catenin that binds F-ACTIN and links the CYTOSKELETON with BETA CATENIN and GAMMA CATENIN.
A multi-functional catenin that is highly homologous to BETA CATENIN. Gamma catenin binds CADHERINS and helps link their cytoplasmic tails to ACTIN in the CYTOSKELETON via ALPHA CATENIN. It is also found in DESMOSOMES where it mediates the link between DESMOSOMAL CADHERINS and DESMOPLAKIN.
Desmoplakins are cytoskeletal linker proteins that anchor INTERMEDIATE FILAMENTS to the PLASMA MEMBRANE at DESMOSOMES.
Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body.
A multi-functional catenin that participates in CELL ADHESION and nuclear signaling. Beta catenin binds CADHERINS and helps link their cytoplasmic tails to the ACTIN in the CYTOSKELETON via ALPHA CATENIN. It also serves as a transcriptional co-activator and downstream component of WNT PROTEIN-mediated SIGNAL TRANSDUCTION PATHWAYS.
Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.
Anchoring points where the CYTOSKELETON of neighboring cells are connected to each other. They are composed of specialized areas of the plasma membrane where bundles of the ACTIN CYTOSKELETON attach to the membrane through the transmembrane linkers, CADHERINS, which in turn attach through their extracellular domains to cadherins in the neighboring cell membranes. In sheets of cells, they form into adhesion belts (zonula adherens) that go all the way around a cell.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
Direct contact of a cell with a neighboring cell. Most such junctions are too small to be resolved by light microscopy, but they can be visualized by conventional or freeze-fracture electron microscopy, both of which show that the interacting CELL MEMBRANE and often the underlying CYTOPLASM and the intervening EXTRACELLULAR SPACE are highly specialized in these regions. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p792)
Adherence of cells to surfaces or to other cells.
A family of proteins that contain several 42-amino acid repeat domains and are homologous to the Drosophila armadillo protein. They bind to other proteins through their armadillo domains and play a variety of roles in the CELL including SIGNAL TRANSDUCTION, regulation of DESMOSOME assembly, and CELL ADHESION.
A group of desmosomal cadherins with cytoplasmic tails that resemble those of classical CADHERINS.
The phenomenon by which dissociated cells intermixed in vitro tend to group themselves with cells of their own type.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS.
The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
Proteins obtained from various species of Xenopus. Included here are proteins from the African clawed frog (XENOPUS LAEVIS). Many of these proteins have been the subject of scientific investigations in the area of MORPHOGENESIS and development.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Established cell cultures that have the potential to propagate indefinitely.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A tumor composed of cells resembling those of the hair matrix, which undergo 'mummification' and may calcify. It is a relatively uncommon tumor, which may occur at any age from infancy. The majority of patients are under 20, and females are affected more than males. The lesion is usually a solitary deep dermal or subcutaneous tumor 3-30 mm in diameter, situated in the head, neck, or upper extremity. (From Rook et al., Textbook of Dermatology, 4th ed, p2401)
A negative regulator of beta-catenin signaling which is mutant in ADENOMATOUS POLYPOSIS COLI and GARDNER SYNDROME.
A malignant neoplasm occurring in young children, primarily in the liver, composed of tissue resembling embryonal or fetal hepatic epithelium, or mixed epithelial and mesenchymal tissues. (Stedman, 25th ed)
A polyposis syndrome due to an autosomal dominant mutation of the APC genes (GENES, APC) on CHROMOSOME 5. The syndrome is characterized by the development of hundreds of ADENOMATOUS POLYPS in the COLON and RECTUM of affected individuals by early adulthood.
A family of DNA-binding proteins that are primarily expressed in T-LYMPHOCYTES. They interact with BETA CATENIN and serve as transcriptional activators and repressors in a variety of developmental processes.
The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.
The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Antibodies produced by a single clone of cells.
Wnt proteins are a large family of secreted glycoproteins that play essential roles in EMBRYONIC AND FETAL DEVELOPMENT, and tissue maintenance. They bind to FRIZZLED RECEPTORS and act as PARACRINE PROTEIN FACTORS to initiate a variety of SIGNAL TRANSDUCTION PATHWAYS. The canonical Wnt signaling pathway stabilizes the transcriptional coactivator BETA CATENIN.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.

Misexpression of the catenin p120(ctn)1A perturbs Xenopus gastrulation but does not elicit Wnt-directed axis specification. (1/407)

Modulators of cadherin function are of great interest given that the cadherin complex actively contributes to the morphogenesis of virtually all tissues. The catenin p120(ctn) (formerly p120cas) was first identified as a src- and receptor-protein tyrosine kinase substrate and later shown to interact directly with cadherins. In common with beta-catenin and plakoglobin (gamma-catenin), p120(ctn) contains a central Armadillo repeat region by which it binds cadherin cytoplasmic domains. However, little is known about the function of p120(ctn) within the cadherin complex. We examined the role of p120(ctn)1A in early vertebrate development via its exogenous expression in Xenopus. Ventral overexpression of p120(ctn)1A, in contrast to beta-catenin, did not induce the formation of duplicate axial structures resulting from the activation of the Wnt signaling pathway, nor did p120(ctn) affect mesoderm induction. Rather, dorsal misexpression of p120(ctn) specifically perturbed gastrulation. Lineage tracing of cells expressing exogenous p120(ctn) indicated that cell movements were disrupted, while in vitro studies suggested that this may have been a consequence of reduced adhesion between blastomeres. Thus, while cadherin-binding proteins beta-catenin, plakoglobin, and p120(ctn) are members of the Armadillo protein family, it is clear that these proteins have distinct biological functions in early vertebrate development. This work indicates that p120(ctn) has a role in cadherin function and that heightened expression of p120(ctn) interferes with appropriate cell-cell interactions necessary for morphogenesis.  (+info)

The catenin p120(ctn) interacts with Kaiso, a novel BTB/POZ domain zinc finger transcription factor. (2/407)

p120(ctn) is an Armadillo repeat domain protein with structural similarity to the cell adhesion cofactors beta-catenin and plakoglobin. All three proteins interact directly with the cytoplasmic domain of the transmembrane cell adhesion molecule E-cadherin; beta-catenin and plakoglobin bind a carboxy-terminal region in a mutually exclusive manner, while p120 binds the juxtamembrane region. Unlike beta-catenin and plakoglobin, p120 does not interact with alpha-catenin, the tumor suppressor adenomatous polyposis coli (APC), or the transcription factor Lef-1, suggesting that it has unique binding partners and plays a distinct role in the cadherin-catenin complex. Using p120 as bait, we conducted a yeast two-hybrid screen and identified a novel transcription factor which we named Kaiso. Kaiso's deduced amino acid sequence revealed an amino-terminal BTB/POZ protein-protein interaction domain and three carboxy-terminal zinc fingers of the C2H2 DNA-binding type. Kaiso thus belongs to a rapidly growing family of POZ-ZF transcription factors that include the Drosophila developmental regulators Tramtrak and Bric a brac, and the human oncoproteins BCL-6 and PLZF, which are causally linked to non-Hodgkins' lymphoma and acute promyelocytic leukemia, respectively. Monoclonal antibodies to Kaiso were generated and used to immunolocalize the protein and confirm the specificity of the p120-Kaiso interaction in mammalian cells. Kaiso specifically coprecipitated with a variety of p120-specific monoclonal antibodies but not with antibodies to alpha- or beta-catenin, E-cadherin, or APC. Like other POZ-ZF proteins, Kaiso localized to the nucleus and was associated with specific nuclear dots. Yeast two-hybrid interaction assays mapped the binding domains to Arm repeats 1 to 7 of p120 and the carboxy-terminal 200 amino acids of Kaiso. In addition, Kaiso homodimerized via its POZ domain but it did not heterodimerize with BCL-6, which heterodimerizes with PLZF. The involvement of POZ-ZF proteins in development and cancer makes Kaiso an interesting candidate for a downstream effector of cadherin and/or p120 signaling.  (+info)

p120(ctn) acts as an inhibitory regulator of cadherin function in colon carcinoma cells. (3/407)

p120(ctn) binds to the cytoplasmic domain of cadherins but its role is poorly understood. Colo 205 cells grow as dispersed cells despite their normal expression of E-cadherin and catenins. However, in these cells we can induce typical E-cadherin-dependent aggregation by treatment with staurosporine or trypsin. These treatments concomitantly induce an electrophoretic mobility shift of p120(ctn) to a faster position. To investigate whether p120(ctn) plays a role in this cadherin reactivation process, we transfected Colo 205 cells with a series of p120(ctn) deletion constructs. Notably, expression of NH2-terminally deleted p120(ctn) induced aggregation. Similar effects were observed when these constructs were introduced into HT-29 cells. When a mutant N-cadherin lacking the p120(ctn)-binding site was introduced into Colo 205 cells, this molecule also induced cell aggregation, indicating that cadherins can function normally if they do not bind to p120(ctn). These findings suggest that in Colo 205 cells, a signaling mechanism exists to modify a biochemical state of p120(ctn) and the modified p120(ctn) blocks the cadherin system. The NH2 terminus-deleted p120(ctn) appears to compete with the endogenous p120(ctn) to abolish the adhesion-blocking action.  (+info)

Functional characterization of multiple transactivating elements in beta-catenin, some of which interact with the TATA-binding protein in vitro. (4/407)

beta-Catenin, a member of the family of Armadillo repeat proteins, plays a dual role in cadherin-mediated cell adhesion and in signaling by Wnt growth factors. Upon Wnt stimulation beta-catenin undergoes nuclear translocation and serves as transcriptional coactivator of T cell factor DNA-binding proteins. Previously the transactivation potential of different portions of beta-catenin has been demonstrated, but the precise location of transactivating elements has not been established. Also, the mechanism of transactivation by beta-catenin and the molecular basis for functional differences between beta-catenin and the closely related proteins Armadillo and Plakoglobin are poorly understood. Here we have used a yeast system for the detailed characterization of the transactivation properties of beta-catenin. We show that its transactivation domains possess a modular structure, consist of multiple subelements that cover broad regions at its N and C termini, and extend considerably into the Armadillo repeat region. Compared with beta-catenin the N termini of Plakoglobin and Armadillo have different transactivation capacities that may explain their distinct signaling properties. Furthermore, transactivating elements of beta-catenin interact specifically and directly with the TATA-binding protein in vitro providing further evidence that a major function of beta-catenin during Wnt signaling is to recruit the basal transcription machinery to promoter regions of Wnt target genes.  (+info)

Nuclear localization of the p120(ctn) Armadillo-like catenin is counteracted by a nuclear export signal and by E-cadherin expression. (5/407)

The Armadillo protein p120(ctn) associates with the cytoplasmic domain of cadherins and accumulates at cell-cell junctions. Particular Armadillo proteins such as beta-catenin and plakophilins show a partly nuclear location, suggesting gene-regulatory activities. For different human E-cadherin-negative carcinoma cancer cell lines we found expression of endogenous p120(ctn) in the nucleus. Expression of E-cadherin directed p120(ctn) out of the nucleus. Previously, we reported that the human p120(ctn) gene might encode up to 32 protein isoforms as products of alternative splicing. Overexpression of p120(ctn) isoforms B in various cell lines resulted in cytoplasmic immunopositivity but never in nuclear staining. In contrast, upon expression of p120(ctn) cDNAs lacking exon B, the isoforms were detectable within both nuclei and cytoplasm. A putative nuclear export signal (NES) with a characteristic leucine-rich motif is encoded by exon B. This sequence element was shown to be required for nuclear export and to function autonomously when fused to a carrier protein and microinjected into cell nuclei. Moreover, the NES function of endogenously or exogenously expressed p120(ctn) isoforms B was sensitive to the nuclear export inhibitor leptomycin B. Expression of exogenous E-cadherin down-regulated nuclear p120(ctn) whereas activation of protein kinase C increased the level of nuclear p120(ctn). These results reveal molecular mechanisms controlling the subcellular distribution of p120(ctn).  (+info)

p120(ctn) binds to the membrane-proximal region of the E-cadherin cytoplasmic domain and is involved in modulation of adhesion activity. (6/407)

Cadherins are transmembrane glycoproteins involved in Ca(2+)-dependent cell-cell adhesion. Previously, we showed that the conserved membrane-proximal region of the E-cadherin cytoplasmic domain negatively regulates adhesion activity. In this report, we provide several lines of evidence that p120(ctn) is involved in this negative regulation. p120(ctn) binds to the membrane-proximal region of the nonfunctional carboxyl-terminally deleted E-cadherin protein. An additional internal deletion in this region prevented the association with p120(ctn) and activated the protein, as seen in an aggregation assay. Furthermore, the nonfunctional E-cadherin can be activated through coexpression of p120(ctn) proteins with amino-terminal deletions, which eliminate several potential serine/threonine phosphorylation sites but do not affect the ability to bind to cadherins. Finally, we show that staurosporine, a kinase inhibitor, induces an increased electrophoretic mobility of p120(ctn) bound to E-cadherin polypeptides, activates the nonfunctional E-cadherin protein, and converts the wild-type E-cadherin and an E-cadherin-alpha-catenin chimeric protein from a cytochalasin D-sensitive to a cytochalasin D-insensitive state. Together, these results indicate that p120(ctn) is a modulator of E-cadherin-mediated cell adhesion.  (+info)

N-Cadherin expression in human prostate carcinoma cell lines. An epithelial-mesenchymal transformation mediating adhesion withStromal cells. (7/407)

In human prostate adenocarcinoma, an association between loss of E-cadherin, increased Gleason score, and extracapsular dissemination has been observed. Further characterization of the E-cadherin/catenin phenotype of human prostate carcinoma cell lines showed loss of E-cadherin and expression of N-cadherin in poorly differentiated prostate carcinoma cell lines (PC-3N derived from PC-3, PC-3, and JCA1). We showed that N-cadherin is concentrated at sites of cell-cell contact in PC-3N cellular extensions. N-cadherin was also expressed in prostate stromal fibroblasts both in vitro and in prostate tissue. Co-cultures of prostate stromal fibroblasts and PC-3N cells showed the immunolocalization of N-cadherin in intercellular contacts. In addition, the isoform expression of the cadherin binding protein p120(ctn) differed in relation to the expression of E- versus N-cadherin by the prostate carcinoma cell lines. The p100 isoform was more highly expressed in E-cadherin-positive carcinoma cell lines, whereas p120 was predominantly expressed only in N-cadherin-positive prostate carcinoma cell lines and prostate stromal fibroblasts. The N-cadherin-positive carcinoma cell line, PC-3N, displayed aggressive invasion into the surface of the diaphragm muscle after intraperitoneal injection of SCID mice. The gain of N-cadherin and loss of E-cadherin by invasive prostate carcinoma cell lines suggests a progression from an epithelial to a mesenchymal phenotype, which may allow for their interaction with surrounding stromal fibroblasts and facilitate metastasis.  (+info)

Epithelial mesenchymal transition by c-Fos estrogen receptor activation involves nuclear translocation of beta-catenin and upregulation of beta-catenin/lymphoid enhancer binding factor-1 transcriptional activity. (8/407)

Mouse mammary epithelial cells expressing a fusion protein of c-Fos and the estrogen receptor (FosER) formed highly polarized epithelial cell sheets in the absence of estradiol. Beta-catenin and p120(ctn) were exclusively located at the lateral plasma membrane in a tight complex with the adherens junction protein, E-cadherin. Upon activation of FosER by estradiol addition, cells lost epithelial polarity within two days, giving rise to a uniform distribution of junctional proteins along the entire plasma membrane. Most of the beta-catenin and p120(ctn) remained in a complex with E-cadherin at the membrane, but a minor fraction of uncomplexed cytoplasmic beta-catenin increased significantly. The epithelial-mesenchymal cell conversion induced by prolonged estradiol treatment was accompanied by a complete loss of E-cadherin expression, a 70% reduction in beta-catenin protein level, and a change in the expression pattern of p120(ctn) isoforms. In these mesenchymal cells, beta-catenin and p120(ctn) were localized in the cytoplasm and in defined intranuclear structures. Furthermore, beta-catenin colocalized with transcription factor LEF-1 in the nucleus, and coprecipitated with LEF-1-related proteins from cell extracts. Accordingly, beta-catenin- dependent reporter activity was upregulated in mesenchymal cells and could be reduced by transient expression of exogenous E-cadherin. Thus, epithelial mesenchymal conversion in FosER cells may involve beta-catenin signaling.  (+info)

Ctnnd1 - Ctnnd1 (Myc-DDK-tagged) - Mouse catenin (cadherin associated protein), delta 1 (Ctnnd1), transcript variant 2 available for purchase from OriGene - Your Gene Company.
Background: Approximately one third of the hereditary diffuse gastric cancer (HDGC) families carry germline mutations in the E-cadherin gene (CDH1). Risk prediction in members of families with this rare but deadly cancer could be improved by the identification of additional HDGC genes in non-CDH1 families.. Methods: Affected individuals from 22 CDH1 mutation-negative families were screened for germline mutations in four catenin genes: CTNNA1, CTNNB1, JUP, and CTNND1. Catenins interact closely with E-cadherin molecules in cells, and are therefore logical candidate genes for mutation screening in HDGC families.. Results: No nonsynonymous variants were seen in CTNNA1, CTNNB1, or CTNND1; only JUP contained nonsynonymous variants, of which only two rare variants were predicted to be deleterious.. Conclusion: Catenin genes are not commonly mutated in non-CDH1 HDGC families.. Impact: Germline mutations in CTNNA1, CTNNB1, JUP, or CTNND1 are unlikely to play a major role in HDGC. Cancer Epidemiol ...
BACKGROUND: Approximately one third of the hereditary diffuse gastric cancer (HDGC) families carry germline mutations in the E-cadherin gene (CDH1). Risk prediction in members of families with this rare but deadly cancer could be improved by the identification of additional HDGC genes in non-CDH1 families.METHODS: Affected individuals from 22 CDH1 mutation-negative families were screened for germline mutations in four catenin genes: CTNNA1, CTNNB1, JUP, and CTNND1. Catenins interact closely with E-cadherin molecules in cells, and are therefore logical candidate genes for mutation screening in HDGC families.RESULTS: No nonsynonymous variants were seen in CTNNA1, CTNNB1, or CTNND1; only JUP contained nonsynonymous variants, of which only two rare variants were predicted to be deleterious.CONCLUSION: Catenin genes are not commonly mutated in non-CDH1 HDGC families.Impact: Germline mutations in CTNNA1, CTNNB1, JUP, or CTNND1 are unlikely to play a major role in HDGC. Cancer Epidemiol Biomarkers Prev; 1-3.
Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as α-catenin and β-catenin. A-catenin can bind to β-catenin and can also bind actin. B-catenin binds the cytoplasmic domain of some cadherins. Additional catenins such as γ-catenin and δ-catenin have been identified. The name catenin was originally selected (catena means chain in Latin) because it was suspected that catenins might link cadherins to the cytoskeleton. α-catenin β-catenin δ-catenin γ-catenin All but α-catenin contain armadillo repeats. Several types of catenins work with N-cadherins to play an important role in learning and memory (For full article, see Cadherin-catenin complex in learning and memory). Cell-cell adhesion complexes are required for simple epithelia in higher organisms to maintain structure, function and polarity. These complexes, which help regulate cell growth in addition to creating and ...
Zinc protoporphyrin (ZnPP) continues to be found to have got anticancer activity both and and [2C5] which is generally believed that ZnPPs anticancer activity is related to HO-1 inhibition. help elucidate the mobile systems of ZnPPs anticancer actions, but provide brand-new cancer therapeutic approaches for concentrating on the -catenin MLN0128 Wnt signaling pathway. In todays study, weve explored the mobile systems of ZnPP-induced suppression of -catenin appearance in human cancer tumor cells. The speedy and dramatic character from the ZnPP-induced suppression of -catenin proteins appearance strongly shows that this suppression is normally primarily because of -catenin proteins degradation. -catenin proteins amounts are well managed with the -catenin devastation complex thats tightly coupled towards the ubiquitin-proteasome program [9]. Hence, it is MLN0128 likely the ubiquitin-proteasome program mediates ZnPP-induced -catenin proteins degradation. However, additional proteins degradation ...
Buy our Recombinant Human delta 1 Catenin protein. Ab114472 is a protein fragment produced in Wheat germ and has been validated in WB, ELISA, SDS-PAGE. Abcam…
Catenins have emerged as molecular sensors that integrate cell-cell junctions and cytoskeletal dynamics with signaling pathways that control morphogenesis and cell to cell communication. δ1-Catenin (p120 catenin) is a catenin family member which contains an N-terminal coiled-coil domain, a regulatory domain containing
Wnt pathway deregulation is a common characteristic of many cancers. But only Colorectal Cancer predominantly harbours mutations in APC, whereas other cancer types (hepatocellular carcinoma, solid pseudopapillary tumours of pancreas) have activating mutations in β-catenin (CTNNB1). We have compared the dynamics and the potency of β-catenin mutations in vivo. Within the murine small intestine (SI), an activating mutation of β-catenin took much longer to achieve a Wnt deregulation and acquire a crypt-progenitor-cell (CPC) phenotype than Apc or Gsk3 loss. Within the colon, a single activating mutation of β-catenin was unable to drive Wnt deregulation or induce the CPC phenotype. This ability of β-catenin mutation to differentially transform the SI versus the colon correlated with significantly higher expression of the β-catenin binding partner E-cadherin. This increased expression is associated with a higher number of E-cadherin:β-catenin complexes at the membrane. Reduction of E-cadherin ...
TY - JOUR. T1 - β-Catenin serves as a clutch between low and high intercellular E-cadherin bond strengths. AU - Bajpai, Saumendra. AU - Feng, Yunfeng. AU - Wirtz, Denis. AU - Longmore, Gregory D.. PY - 2013/11/19. Y1 - 2013/11/19. N2 - A wide range of invasive pathological outcomes originate from the loss of epithelial phenotype and involve either loss of function or downregulation of transmembrane adhesive receptor complexes, including Ecadherin (Ecad) and binding partners β-catenin and α-catenin at adherens junctions. Cellular pathways regulating wild-type β-catenin level, or direct mutations in β-catenin that affect the turnover of the protein have been shown to contribute to cancer development, through induction of uncontrolled proliferation of transformed tumor cells, particularly in colon cancer. Using single-molecule force spectroscopy, we show that depletion of β-catenin or the prominent cancer-related S45 deletion mutation in β-catenin present in human colon cancers both weaken ...
In metazoan adherens junctions, β-catenin links the cytoplasmic tail of classical cadherins to the F-actin-binding protein α-catenin. Phosphorylation of a Ser/Thr-rich region in the cadherin tail dramatically enhances affinity for β-catenin and promotes cell-cell adhesion in cell culture systems, but its importance has not been demonstrated in vivo. In this paper, we identify a critical phosphorylated serine in the C. elegans cadherin HMR-1 required for strong binding to the β-catenin homolog HMP-2. Ablation of this phosphoserine interaction produces developmental defects that resemble full loss-of-function (Hammerhead and Humpback) phenotypes. Most metazoans possess a single gene for β-catenin, which is also a transcriptional coactivator in Wnt signaling. Nematodes and planaria, however, have a set of paralogous β-catenins; for example, C. elegans HMP-2 functions only in cell-cell adhesion, whereas SYS-1 mediates transcriptional activation through interactions with POP-1/Tcf. Finally, our ...
When we examined solid pseudopapillary tumours of the pancreas, a tumour characterised by β‐catenin mutations within exon 3, these tumours showed a strong reduction in E‐cadherin:β‐catenin complexes. Moreover in HCC, a tumour type which has approximately 20% β‐catenin exon 3 mutations, there was a good correlation between reduction in E‐cadherin and activation of Wnt signalling targets. Thus, it may be that in these cancers, E‐cadherin limits the precise levels of Wnt signalling driven by β‐catenin mutation. Thus, downregulation of E‐cadherin in these tumours may drive tumour progression. It should also be noted that opposing patterns of Wnt signalling and E‐cadherin have been shown in murine liver, with β‐catenin higher in zone 3 of the liver and E‐cadherin in zone 1. Therefore, one might predict that β‐catenin mutations would yield a greater phenotype in hepatocytes from zone 3 of the liver versus zone 1 (Benhamouche et al, 2006). Hence, one could speculate ...
Objective: The aim of this study is to verify if oxidative stress is related to changes in content and pattern of β-catenin protein expression in an experimental model of diversion colitis. Methods: Sixty Wistar rats were submitted to intestinal bypass. The animals were divided into three groups according to the sacrifice to take place in six, 12 and 18 weeks. For each group, five animals only underwent laparotomy (control). The presence of colitis was diagnosed by histological study, and its severity, by inflammation grading scale. Cellular oxidative stress was measured by comet assay. Tissue expression of β-catenin protein was analyzed by the immunohistochemistry and quantification of its tissue content by computerized morphometry. Statistical analysis was performed with the Students t-test, median, Mann-Whitney, ANOVA and Kruskal-Wallis, adopting a significance level of 5% (p ,0.05). Results: Colon segments without fecal stream developed colitis, which worsened with time of exclusion. ...
The KOMP Repository Collection is located at the MMRRC at the University of California, Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
TGF-β1 has been previously reported to promote tyrosine phosphorylation of β-catenin (Tian and Phillips, 2002), although the specific site of phosphorylation and its functional significance in TGF-β1 signaling has been unknown. Our finding of integrin-dependent tyrosine phosphorylation of Y654-β-catenin is important because phosphorylation of β-catenin at Y654 is known to promote both dissociation of β-catenin from E-cadherin and stabilization of β-catenin from ubiquitination and degradation (Brembeck et al., 2006). Therefore, independently of Wnt signaling, our findings indicate that TGF-β1 can promote a pathway of cross talk with β-catenin by generating stable pY654-β-catenin-Smad complexes. The data indicate that only a fraction of the β-catenin is phosphorylated, and presumably, this reflects, at least in part, the pool internalized with E-cadherin and TGF-βR1 after TGF-β1 stimulation. However, internalization alone does not appear to be sufficient, as α3-null cells, even ...
Aberrant regulation of the Wnt/β-catenin pathway plays important roles in colorectal carcinogenesis, with over 90% of cases of sporadic colon cancer featuring β-catenin accumulation. While ubiquitination-mediated degradation is widely accepted as a major route for β-catenin protein turnover, little is known about the regulation of β-catenin in transcriptional level. …Elf3, a member of the E-twenty-six family of transcription factors, drives β-catenin transactivation and associates with poor survival of colorectal cancer (CRC) patients. … first found recurrent amplification and upregulation of Elf3 in CRC tissues, and further Gene Set Enrichment Analysis identified significant association between Elf3 expression and activity of WNT/β-catenin pathway. Chromatin immunoprecipitation and electrophoretic mobility shift assay consistently revealed that Elf3 binds to and transactivates β-catenin promoter. Ectopic expression of Elf3 induces accumulation of β-catenin in both nucleus and ...
Regulating the cytosolic concentration of the protein β-catenin is an important cell proliferation control mechanism. The cytoplasmic concentration of β-catenin remains low through an interaction with a protein complex consisting of adenomatous polyposis coli (APC), Axin, protein phosphatase 2A, and glycogen synthase kinase 3β (GSK3β). Upon phosphorylation by GSK3β, β-catenin associates with a ubiquitin ligase (E3) complex, resulting in its ubiquitination and proteolysis. Activation of Wnt signaling inactivates GSK3, allowing β-catenin to accumulate in the cytoplasm and eventually to translocate to the nucleus so as to affect gene expression. Two groups report that a phosphorylation-independent mechanism can lead to the destruction of β-catenin. Matsuzawa and Reed show that a mammalian protein called Siah-1 lies at the hub of another pathway that destroys β-catenin in response to DNA damaging agents and the activation of the tumor suppressor protein p53. Siah-1 is known to interact with ...
Clone REA548 recognizes the S879-phosphorylated form of p120 catenin, a prototypic member of the subfamily of armadillo-domain (ARM) proteins. p120 ARM domain displays 22% homology to other ARM-domain proteins such as β-catenin and plakoglobin, which are basically cadherin binding proteins, suggesting the involvement of p120 in binding to cadherin. p120 associates with most classical (type I) and non-classical (type II) cadherins via ARM repeats 1-7 and is localised at the cell junctions, where it constitutes the cadherin complex. Functions of p120 include regulation of stability of cadherins and modulation of activity of rho GTPases in the cytoplasm. Furthermore, lack of p120 catenin in mouse leads to tumor development, suggesting its further role as tumor supressor. Phosphorylation of p120 catenin at multiple sites regulates its various functions and can be phosphorylated by Src and various receptor tyrosine kinases. Additional information: Clone REA548 displays negligible binding to Fc receptors. -
Clone REA548 recognizes the S879-phosphorylated form of p120 catenin, a prototypic member of the subfamily of armadillo-domain (ARM) proteins. p120 ARM domain displays 22% homology to other ARM-domain proteins such as β-catenin and plakoglobin, which are basically cadherin binding proteins, suggesting the involvement of p120 in binding to cadherin. p120 associates with most classical (type I) and non-classical (type II) cadherins via ARM repeats 1-7 and is localised at the cell junctions, where it constitutes the cadherin complex. Functions of p120 include regulation of stability of cadherins and modulation of activity of rho GTPases in the cytoplasm. Furthermore, lack of p120 catenin in mouse leads to tumor development, suggesting its further role as tumor supressor. Phosphorylation of p120 catenin at multiple sites regulates its various functions and can be phosphorylated by Src and various receptor tyrosine kinases. Additional information: Clone REA548 displays negligible binding to Fc receptors. -
A151 Vitamin D is implicated as a protective factor in human colon carcinogenesis. Anti-tumor activity of vitamin D on colonocytes is thought to be mediated by 1,25 (OH)2D3, the active hormonal metabolite of vitamin D. We examined the role of 1,25 (OH)2D3on a putative VDR/β-catenin interaction. We first sought to confirm that VDR associates physically with β-catenin regulating critical Wnt signaling events in colon tumorigenesis. Second, we assessed the role of the intact and mutant adenomatous polyposis coli (APC) gene, commonly dysregulated in human colon cancers, as a modulator of the VDR/β-catenin interaction. Glutathione-S-transferase (GST) pulldown assays and an in-vitro transcription/translation system to produce β-catenin protein support an interaction between β-catenin and VDR that appears independent of 1,25 (OH)2D3. Further evaluation of this interaction within the cell using a mammalian two hybrid system in transfected HT29-APC, a cell line which allows inducible wildtype APC ...
The present study identified five new β-catenin target genes in thalamic neurons, in addition to previously described Cacna1g[16]. Three of them, Kcna6, Calb2, and Gabra3, were validated by ChIP in vivo and a loss-of-function experiment in cultured neurons, confirming that they might be directly regulated by β-catenin. Two other genes, Cacna2d2 and Kcnh8, also displayed β-catenin-dependence in the latter experiment, although the binding of β-catenin to their regulatory elements was not found. Based on these data, we propose that β-catenin is a regulator of the electrophysiological properties of thalamic neurons in the adult brain.. Numerous genes that we selected in silico as potentially regulated by β-catenin belong to expected functional categories: transcription regulation, cell proliferation, morphogenesis, motility, adhesion, differentiation, and programmed cell death. Similar clusters were observed by others in the genes bound by TCF7L2 in a human colorectal cancer cell line [40]. ...
Our study unveils a TREM2/β-catenin pathway that regulates bone mass by regulating the rate of OC generation. Mechanistically, TREM2 and β-catenin augment the M-CSF-induced proliferation of OcP, retarding their differentiation into mature OC. Ablation of either TREM2 or β-catenin inhibits the proliferation of OcP, accelerating their differentiation into bone-resorbing OC, which ultimately cause osteoporosis. The possibility that TREM2 and β-catenin act along the same pathway is supported not only by the similar osteoporotic phenotypes of TREM2−/− and βcatΔ/Δ mice, but also by genetic evidence that simultaneous heterozygosity for TREM2- and β-catenin-null alleles results in osteoporosis, whereas no phenotype is observed in mice heterozygous for either of these alleles.. TREM2 may enhance M-CSF-induced activation of β-catenin by facilitating the recruitment of DAP12 to the receptor for M-CSF. In turn, DAP12 may activate Syk and Pyk2, which promote phosphorylation and nuclear ...
Vascular and neuronal networks share a similar branching morphology, and emerging evidence implicates common mechanisms in the formation of both systems. δ-Catenin is considered a neuronal catenin regulating neuron cell-cell adhesion and cell motility. Here, we report expression of δ-catenin in vascular endothelium, and show that deletion of only one allele of δ-catenin is sufficient to impair endothelial cell motility and vascular assembly in vitro and pathological angiogenesis in vivo, thereby inhibiting tumor growth and wound healing. In contrast, deletion of one or both allele of δ-catenin had no effects on hormone-induced physiological angiogenesis in the uterus. Molecular analysis confirmed a gene dosage effect of δ-catenin on Rho GTPase activity. Moreover, we show that inflammatory cytokines, but not angiogenic factors, regulate δ-catenin expression, and the levels of δ-catenin positively correlate to human lung cancers. Collectively, our data suggest that inflammation, commonly ...
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gamma-catenin Antibody (F-2) is a monoclonal anti-γ-catenin antibody that detects m, r, and h γ-catenin by WB, IP, IF and ELISA.
β-Catenin is essential for embryonic development and required for cell renewal/regeneration in adult life. Cellular β-catenin exists in three different pools: membranous, cytoplasmic and nuclear. In...
Mutations in the Wnt/-catenin pathway occur in most colorectal cancers (CRCs), and these mutations lead to increased nuclear accumulation of the -catenin transcriptional co-activator. element within the first intron of the gene to drive expression in CRC cells. As such, reducing -catenin expression in CRC cells using shRNAs leads to decreased mRNA and protein levels. …Read More. ...
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Absence of β-catenin does not affect spontaneous or glucocorticoid-induced death of thymocytes. (a) Percentage of surviving thymocytes gated on CD45.1+ (WT don
Normal development of β-catenin−/− hematopoietic lineages in a competitive situation. Mixed BM chimeric mice were analyzed 4-6 mo after reconstitution wi
A bunch of us were trying to watch Duck Soup last night as a guy provided unsolicited Marxist subtextual analysis. It was quite illuminating. For example, I had never realized that Edgar Kennedy, selling his lemonade, represented Capital while Harpo, the peanut vendor (or El Manicero, badly whistled), was Labor. Their struggle was represented brilliantly by…
The canonical WNT-β-catenin pathway is essential for self-renewal, growth and survival of AML stem/blast progenitor cells (BPCs). Deregulated WNT signaling inhibits degradation of β-catenin, causing increased nuclear translocation and co-factor activity of β-catenin with the transcriptional regulator TCF4/LEF1 in AML BPCs. Here, we determined the pre-clinical anti-AML activity of the ...
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The catenins are polypeptides that bind to the conserved cytoplasmic tail of cadherins and are required for cadherin function. α-Catenin is related to vinculin and seems to be required for the interaction of cadherins with the actin cytoskeleton. β-Catenin is homologous to armadillo, a segment polarity gene in Drosophila that participates in developmental signaling. Recent findings indicate that β-catenin also participates in developmental signaling and embryonic patterning in Xenopus laevis. At least a portion of the electrophoretic band migrating at the position of γ-catenin consists of plakoglobin, a desmosomal and zonula adherens protein that has high sequence similarity to β-catenin and armadillo. The catenins may be involved in the regulation of cadherin function during tissue morphogenesis and tumorigenesis. ...
TY - JOUR. T1 - Expression of cadherins and catenins in paired tumor and non-neoplastic primary prostate cultures and corresponding prostatectomy specimens. AU - Wang, J.. AU - Krill, D.. AU - Torbenson, Michael. AU - Wang, Q.. AU - Bisceglia, M.. AU - Stoner, J.. AU - Thomas, A.. AU - DeFlavia, P.. AU - Dhir, R.. AU - Becich, M. J.. PY - 2000/1/1. Y1 - 2000/1/1. N2 - Cadherins are a family of transmembrane proteins that play a crucial role in cell differentiation, cell migration, and intercellular adhesion. Cadherins are associated with catenins through their highly conserved cytoplasmic domain. Down-regulation of E-cadherin protein has been shown in various human cancers. This study examined the expression of cadherins and associated catenins at the mRNA level. Paired tumor and non-neoplastic primary prostate cultures were obtained from surgical specimens. Quantitative multiplex fluorescence reverse transcriptase-polymerase chain reaction (QMF RT-PCR) and quantitative analysis were performed ...
Cadherins represent a distinct family of single transmembrane domain glycoproteins that mediate calcium-dependent cell-cell adhesion via homophilic interactions of their NH2-terminal ectodomains (Shapiro et al., 1995; Gumbiner, 2000). The intracellular domain of E-cadherin associates with a protein family collectively termed catenins (Ozawa et al., 1989; Nathke et al., 1994). β-catenin and γ-catenin (plakoglobin) interact directly with E-cadherins COOH-terminal domain in a mutually exclusive way, and both proteins associate with α-catenin, which links the cadherin complexes to the actin cytoskeleton and mediates stable cell adhesion. p120ctn is another catenin family member (Anastasiadis and Reynolds, 2000), which binds to the cytoplasmic juxtamembrane portion of E-cadherin and influences E-cadherin clustering and adhesive strength (Ozawa and Kemler, 1998; Yap et al., 1998; Aono et al., 1999; Ohkubo and Ozawa, 1999; Thoreson et al., 2000).. In addition to its adhesive functions, β-catenin ...
Known proteins from the cell-adhesion protein E-cadherin include proteins and catenins involved with signaling trafficking and actin organization. catenins and almost 40 others that were previously reported to influence cadherin function. Many others could be rationalized as novel candidates for regulating the adherens junction Isovitexin cytoskeleton trafficking or signaling. We further characterized lipoma desired partner (LPP) which is present at both cell contacts and focal adhesions. Knockdown of LPP shown its requirement for E-cadherin-dependent adhesion and suggested that it plays a role in coordination of the cell-cell and cell-substrate cytoskeletal relationships. The analysis of LPP function demonstrates proof of principle the proteomic analysis of E-cadherin proximal proteins expands the inventory of parts and tools for understanding the function of E-cadherin. proximity ligation assay (PLA); this assay results in the production of a fluorescent transmission when antibodies to two ...
The sys-1 gene encodes a highly divergent β-catenin (6, 8). Several lines of evidence support the idea that SYS-1 is a functional β-catenin. First, transgenic SYS-1 can rescue a null mutant of bar-1, which encodes a typical β-catenin. Second, SYS-1 binds the β-catenin binding domain of POP-1/TCF. Third, SYS-1 acts as a transcriptional coactivator for POP-1 in a TOPFLASH reporter. Fourth, ceh-22 expression in distal SGP daughters depends on POP-1 binding sites in the ceh-22b promoter and also on SYS-1 and POP-1. Therefore, SYS-1 acts in many ways like a typical β-catenin.. Both SYS-1/β-catenin and POP-1/TCF control the SGP asymmetric cell division (7, 9). In pop-1 mutants, as in sys-1 mutants, the SGP daughters both adopt a proximal fate (Fig. 1B). Importantly, POP-1 is asymmetrically distributed to SGP daughters (10), a phenomenon that has been seen in many asymmetric cell divisions and has been dubbed POP-1 asymmetry (11). Although counterintuitive, nuclear POP-1 is reduced in distal ...
β-Catenin is currently believed to be involved in the development of colorectal cancer. Aberrant expression of the APC gene and mutations at the site of phosphorylation of β-catenin are the two major factors that account for the overexpression and subsequent cytoplasmic/nuclear translocation of β-catenin found in colorectal carcinoma cells. According to in vivo and in vitro data collected by others and us (23 , 26) , the accumulation of cytoplasmic β-catenin does not always correlate with nuclear expression of the protein. Because the nuclear entry of β-catenin is an obvious step required for gene activation induced by the β-catenin/T-cell factor/Lef-1 transcriptional complex, we used nuclear staining as primary evidence for β-catenin overexpression. Our data revealed a remarkable correlation between β-catenin nuclear translocation and the purported sequential stages of colorectal cancer development in both Group 1 (240 cases) and group 2 (40 cases) specimens (P , 0.0001 for both groups; ...
The advanced phases of chronic myeloid leukemia (CML) are known to be more resistant to therapy. This resistance has been associated with the overexpression of ABCB1, which gives rise to the multidrug resistance (MDR) phenomenon. MDR is characterized by resistance to nonrelated drugs, and P-glycoprotein (encoded by ABCB1) has been implicated as the major cause of its emergence. Wnt signaling has been demonstrated to be important in several aspects of CML. Recently, Wnt signaling was linked to ABCB1 regulation through its canonical pathway, which is mediated by β-catenin, in other types of cancer. In this study, we investigated the involvement of the Wnt/β-catenin pathway in the regulation of ABCB1 transcription in CML, as the basal promoter of ABCB1 has several β-catenin binding sites. β-catenin is the mediator of canonical Wnt signaling, which is important for CML progression. In this work we used the K562 cell line and its derived MDR-resistant cell line Lucena (K562/VCR) as CML study models. Real
Nasopharyngeal carcinoma has a high incidence in southern China. The Wnt/β-catenin signaling pathway plays a major role in cancer development and progression. Our current study aims to determine the clinical significance of the Wnt/β-catenin pathway components such as β-catenin, cyclooxygenase 2, cyclin D1, c-Myc, and E-cadherin in 148 nasopharyngeal carcinomas by immunohistochemistry. We found that nasopharyngeal carcinoma stage T3+T4 had significantly higher expression of β-catenin, cyclooxygenase 2, cyclin D1, and c-Myc and lower expression of E-cadherin than nasopharyngeal carcinoma stage T1+T2 (P | .001, P | .05, respectively).There was significantly higher expression of β-catenin (P = .001) and cyclooxygenase 2 (P = .003) and lower expression of E-cadherin (P = .001) in nasopharyngeal carcinoma with lymph node metastasis than in nasopharyngeal carcinoma without lymph node metastasis. The expression of β-catenin in nasopharyngeal carcinoma was positively correlated with cyclooxygenase 2 (r =
TY - JOUR. T1 - Pro-tumorigenic phosphorylation of p120 catenin in renal and breast cancer. AU - Kourtidis, Antonis. AU - Yanagisawa, Masahiro. AU - Huveldt, Deborah. AU - Copland, John A.. AU - Anastasiadis, Panos Z.. PY - 2015/6/11. Y1 - 2015/6/11. N2 - Altered protein expression and phosphorylation are common events during malignant transformation. These perturbations have been widely explored in the context of E-cadherin cell-cell adhesion complexes, which are central in the maintenance of the normal epithelial phenotype. A major component of these complexes is p120 catenin (p120), which binds and stabilizes E-cadherin to promote its adhesive and tumor suppressing function. However, p120 is also an essential mediator of pro-tumorigenic signals driven by oncogenes, such as Src, and can be phosphorylated at multiple sites. Although alterations in p120 expression have been extensively studied by immunohistochemistry (IHC) in the context of tumor progression, little is known about the status and ...
Project A4: Mechanisms of post‐transcriptional regulation of plakophilin 1 (PKP1) expression and its role in carcinogenesis. Tina studied Biochemistry at the Martin-Luther-University of Halle-Wittenberg. As a PhD student she investigates the posttranscriptional regulation of p120-cell contact proteins by microRNAs. The members of the p120-catenin subfamily of armadillo proteins are essential for cell-cell contact formation. Besides this function in cell-cell adhesion, they participate in various signaling pathways. Their deregulated expression with up- as well as down-regulation has been connected to cancer and metastasis. Because of this it is important to understand how their expression is controlled at the post-transcriptional level by microRNAs and RNA-binding proteins. For example the RNA-binding protein FMRP associates with and inhibits the translation of the p0071 mRNA. In this way FMRP can regulate the actin cytoskeleton in fibroblasts and neuronal cells via the armadillo protein ...
Pancreatic β-cells are highly responsive to changes in glucose, but the mechanisms involved are only partially understood. There is increasing evidence that the β-catenin signalling pathway plays an important role in regulating β-cell function, but the mechanisms regulating β-catenin signalling in these cells is not well understood. In the present study we show that β-catenin levels and downstream signalling are regulated by changes in glucose levels in INS-1E and β-TC6-F7 β-cell models. We found a glucose-dependent increase in levels of β-catenin in the cytoplasm and nucleus of INS-1E cells. Expression of cyclin D1 also increased with glucose and required the presence of β-catenin. This was associated with an increase in phosphorylation of β-catenin on Ser552, which is known to stabilize the molecule and increase its transcriptional activity. In a search for possible signalling intermediates we found forskolin and cell-permeable cAMP analogues recapitulated the glucose effects, ...
Synapses are fundamental building blocks of neural circuits. Synapse formation requires complex regulation involving cell adhesion molecules, secreted molecules, transcription factors and so forth. For cell adhesion molecules, ...
Figure 1: Fast and late regulation of the activity of GSK3 on β-catenin and the stability of this protein. For more information, Vinyoles et al (2014).. Once released from E-cadherin, p120-catenin participates in other processes necessary for the complete transcriptional activity of the β-catenin/Tcf-4 complex. On the one hand, it facilitates the activation of Rac1, necessary for the stimulation of the JNK and the transport of β-catenin to the nucleus; on the other, it eliminates the repression caused by the Kaiso transcriptional factor, binding directly with that factor.. The CK1e activation mechanism has also been determined which depends on the dephosphorylation of this protein kinase by the phosphatase PP2A. The formation of the complex between the Fz receptor and the LRP5/6 co-receptor, induced by the Wnt3a ligand, enables CK1e,linked indirectly to LRP5/6, to approach PP2A phosphatase, which is associated with Fz through the regulatory subunit PR61e. This facilitates the ...
In cells not exposed to the signal, β-catenin levels are kept low through interactions with the protein kinase zw3/GSK-3, CK1a, APC and Axin (Behrens, 1998 Itoh 1998., Hamada, 1999.) β-catenin is degraded, after phosphorylation by GSK-3 and CK1 alpha (Yanagawa 2002, Liu 2002, Amit 2002), through the ubiquitin pathway (Aberle 1997.), involving interactions with Slimb/bTrCP (Jiang 1998, Marikawa 1998,; reviewed in Maniatis 1999). In a current model, Wnt signaling initially leads to a complex between Dsh, GBP/Frat1, Axin and Zw3/GSK, which may be the regulatory step in the inactivation of Zw3/GSK (Salic, 2000; Farr 2000). The DIX domain in Axin is similar to the NH2 terminus in Dsh, and promotes interactions between Dsh and Axin (Hsu 1999, Smalley, 1999). As a consequence, GSK does not phosphorylate β-catenin anymore, releasing it from the Axin complex and accumulation (Salic, 2000).The stabilized β-catenin then enters the nucleus to interact with TCF. β-catenin can convert TCF into a ...
The most cogent studies supporting the S45 primer model used exogenously overexpressed β-catenin mutants and did not examine cells of intestinal epithelial origin, the major cell type in which disruption of this pathway results in neoplasia. We reasoned that naturally occurring mutations of the β-catenin gene in colorectal cancers could provide a unique opportunity to address this model in cells of colorectal epithelial origin under near physiological conditions (i.e., without overexpression of exogenous proteins). It was reported previously that the colorectal cancer cell line LS 174T harbors a mutation of β-catenin at codon 45 (S45F). We confirmed the presence of the S45F mutation in LS 174T and further determined that these cells lacked a WT 3 allele (Fig. 1A) ⇓ . Accordingly, the S45 primer model would suggest that phosphorylation of β-catenin at S33, S37, or T41 should be absent or greatly diminished in these cells. Indeed, previous studies using overexpressed mutant S45F β-catenin ...
Aberrant activation of the Wnt-β-catenin pathway has been found in a wide range of cancers, especially in cancers derived from intestine, skin, mammary gland and haematopoietic cells. Moreover, the Wnt-β-catenin pathway may preferentially influence stem/progenitor cell expansion in these cancers (Wend et al., 2010). Although many downstream target genes for both normal development and tumorigenesis have been identified in different cellular contexts, the genes that mediate the Wnt-β-catenin pathway activity in maintaining the stem cell properties are still not very clear. c-Myc, a reprogramming factor and a well-known Wnt target, was recently demonstrated to be an important stem cell regulator in normal and cancerous cells (Kim et al., 2010; Smith et al., 2011). However, no study has established a convincing relationship between the Wnt-β-catenin pathway and c-Myc in stem cells. In contrast, recent studies on both iPS and ESCs suggest that the role of the Wnt-β-catenin pathway in ...
In the present study, we provide strong evidence that β-catenin-mediated transcription plays a central role in the transition of tumor-inhibiting M1-like TAMs to tumor-promoting M2-like TAMs. Therefore, targeting β-catenin in TAMs may provide a new immunotherapeutic option to reactivate antitumor immunity in lung TME. This concept (summarized in Fig. 6H) is based on the following key findings. First, Wnt/β-catenin signaling (expression and nuclear β-catenin activity) is significantly activated in TAMs isolated from patients with lung cancer and in M2-like TAMs trained by coculturing with primary lung cancer cells or a lung cancer cell line compared with their M1 counterparts. Second, genetic or pharmacological ablation of nuclear β-catenin activity in primary TAMs isolated from human and mouse lung tumors, as well as in in vitro trained M2-like TAMs, phenotypically and functionally reprograms M2-like TAMs to M1-like TAMs. Third, pharmacological and macrophage-specific genetic ablation ...
The present invention relates to peptides that affect the interaction of the protein β-catenin with a transcription factor or a tumor suppressor protein. Such peptides are derived either from the amino acids of β-catenin that are known to interact with such proteins, or from the amino acids of the transcription factor or tumor suppressor protein that are known to interact with β-catenin. The effect of the interaction is preferably inhibition of binding of β-catenin and the transcription factor or tumor suppressor protein. Peptide mutants and peptidomimetic compounds are also contemplated in the invention. The peptides, mutands and peptidomimetic compounds of the invention are useful for treating cancer in mammals, and particularly in humans.
Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the
beta Catenin (phospho Thr41/Ser45) antibody (catenin (cadherin-associated protein), beta 1, 88kDa) for WB. Anti-beta Catenin (phospho Thr41/Ser45) pAb (GTX50180) is tested in Human samples. 100% Ab-Assurance.
beta Catenin antibody [1F3] (catenin (cadherin-associated protein), beta 1, 88kDa) for IHC-P, WB. Anti-beta Catenin mAb (GTX84650) is tested in Human, Monkey samples. 100% Ab-Assurance.
Cadherins are a family of transmembrane proteins formed from multiple repeats of cadherin-specific motif (a recurrent molecular sequence) and also share a large extracellular domain. Cadherins are classified into two groups: Classical Cadherins and Protocadherins. The main difference between the two groups of cadherins is the classical cadherins contain five cadherin repeats with the third (EC3) and the fifth (EC5) repeat having very specific features. The protocadherins do not share the same features of the EC3 and EC5 units; are longer than five repeats long; and the sequences are very similar to each other. As a result of these differences, the classical cadherins are very specific and do not adhere to a large number of different ECM proteins, whereas protocadherins are much more flexible in their attachments. Classical cadherins are known to only be found in vertebrates so far, while protocadherins are found in planaria, hydra, Drosophila, and various mammals. Cadherins rely heavily on ...
β-Catenin expression was observed in the membrane and/or cytoplasm without any significant nuclear expression. HER-2/neu and EGFR were observed on the membrane in 21% and 6% of tumors, respectively, and Met stained in a membrane/cytoplasm distribution in 28% of cases. Cyclin D1 was expressed in the nucleus and MMP7 was expressed in the cytoplasm in 26% and 75% of tumors, respectively. Nuclear expression of p53 was noted in 31% of tumors. When each marker was analyzed separately, only p53 and Met demonstrated a significant correlation with survival. However, patients who had tumors that coexpressed high levels of β-catenin and p53 had markedly worse overall survival (P = 0.0026). In multivariate analysis, only tumor size, Met, and the coexpression of β-catenin and p53 retained statistical significance. ...
THE EXISTENCE OF CTNND1 IN THIS RENDITION OF GENES IS NOTABLE, THAT IS THAT SOMEWHERE ALONG THE LINE IMMUNOTHERAPY OR ANTI-ROS-1 MAY HAVE A ROLE IN ANAPLASTIC CANCERS WHICH OVER EXPRESS THE ROS-1 MUTATION! IN ITS METASTATIC PHASE THAT IS. activation of β-catenin could represent another mechanism leading to the ATC (THYROID) phenotype. Indeed in one study, such mutations were found in 61% of the lesions but not in the precursor lesions (HEBRANT ET AL ...
The Wnt/β-catenin pathway regulates the cell growth and survival following radiation in various types of cancer cells. Our previous report show that activation of the Wnt/β-catenin signaling pathway is a key radioprotective mechanism in irradiated head and neck cancer (HNC) cells. However, the molecular mechanisms by which β-catenin regulates radiation sensitivity are not clear. Here we attempted to elucidate the mechanism of cell death following radiation by studying how β-catenin silencing controls the radiation sensitivity of radioresistant HNC cells.. Of nine cell lines examined, the most radioresistant cell line (AMC-HN-9) were selected for this experiments. β-catenin silencing using small interfering RNA(siRNA) down-regulated β-catenin expression up to 72 h, which was confirmed by western blot analysis. The sensitivity to radiation was anlayzed by clonogenic analysis and MTT assay. As a result, β-catenin silencing remarkably decreased the survival of irradiated AMC-HN-9 cells and ...
Background: β‐Catenin is an important signaling molecule in the Wnt pathway that plays a key role in tumorgenesis. In the absence of Wnt signaling, the cytoplasmic level of β‐catenin is kept low due to rapid proteasomal‐mediated degradation of GSK3β phosphorylated β‐catenin. Activation of Wnt signaling leads to the inactivation of GSK3β, resulting in stabilization and accumulation of β‐catenin in the cytoplasm. Consequently, β‐catenin translocates into the nucleus, where it binds with members of the T‐cell factor (Tcf)/lymphocyte enhancer‐binding factor family of transcription factors and activates the expression of many target genes important for cancer development. Most colon cancers have activating mutations in the APC tumor suppressor or in β‐catenin itself. Furthermore, activating β‐catenin mutations have been found in a variety of other tumors such as melanomas, hepatocellular carcinomas, skin, breast, and prostate cancer, whereas β‐catenin is not activated ...
beta Catenin, clone: 15B8, eBioscience™ 100μg; Unconjugated beta Catenin, clone: 15B8, eBioscience™ Primary Antibodies Cas to Caz
FHF3 span between extending and Be anxious image resolution (Fig. 3C). Measurements at 10s periods offered identical outcomes STF-62247 (Fig. H4G). Pretreatment with GdCl3 to stretch-loading got no impact prior, and dominated out advantages from stretch-activated Ca2+ stations (not really demonstrated). Cadherin inactivation with obstructing DECMA-1 antibody ablated the response (Fig. 3D). This instant, reversible switching suggests that -catenin features like an flexible springtime in series with the cytoskeleton, which deforms with the extracellular substrate and matrix. Assessment of the nanoprobe and MTC measurements shows that the Be anxious/ECFP reduce during bead rotating (Fig. 2B) can be credited to constant, mechanised perturbation than biochemical adaptation rather. Shape 3 Exogneous mechanised extend induce instant, reversible -catenin conformation switching at intercellular junctions Biochemical indicators STF-62247 during the 3s span between base extend and image resolution could ...
human SVH protein: A specific splicing variant of SVH, a novel human armadillo repeat protein, is up-regulated in hepatocellular carcinomas; amino acid sequence in first source
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Polyclonal antibody for ALPHA 1 CATENIN/CTNNA1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. ALPHA 1 CATENIN/CTNNA1 information: Molecular Weight: 100071 MW; Subcellular Localization: Isoform 1: Cytoplasm,
β-Catenin is an intracellular molecule involved in cell adhesion, cell signaling, and regulation of gene transcription and is implicated in a wide …
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B-catenin[edit]. Main article: Beta-catenin. β-catenin of the canonical Wnt signalling pathway plays a role in cell fate ...
Takemaru, Ken-Ichi (2006). "Catenin, beta". AfCS-Nature Molecule Pages. doi:10.1038/mp.a000506.01. Rousseau, Simon (2011). "P38 ...
CTNNB1 (beta-catenin; a transcription gene) mutations are found in 14-44% of endometrial cancers and may indicate a good ... Beta-catenin mutations are commonly found in endometrial cancers with squamous cells. FGFR2 mutations are found in ... The CTNNB1 (beta-catenin) gene is most commonly mutated in the squamous subtype of endometrioid adenocarcinoma. Serous ...
"Axin-independent phosphorylation of APC controls beta-catenin signaling via cytoplasmic retention of beta-catenin". Biochem. ... "Entrez Gene: catenin". Tago K, Nakamura T, Nishita M, Hyodo J, Nagai S, Murata Y, Adachi S, Ohwada S, Morishita Y, Shibuya H, ... Beta-catenin-interacting protein 1 is a protein that is encoded in humans by the CTNNBIP1 gene. The protein encoded by this ... Reichel O, Mayr D, Durst F, Berghaus A (2008). "E-cadherin but not beta-catenin expression is decreased in laryngeal biopsies ...
APC was found to associate with catenins. Today we know that the beta-catenin protein (part of the Wnt signaling pathway) is ... A mouse model with deregulation of beta-catenin levels was created. The conditional stabilizing mutation in the beta-catenin ... there was substantial nuclear localization of beta-catenin as well as increased cytoplasmic beta-catenin. However, in mice fed ... Furthermore, when evaluated for ERCC1, beclin-1, and beta-catenin in the stem cell region of crypts, the colonic tissues of ...
Catenins are proteins that associate with the cytoplasmic domain of a variety of cadherins. The association of catenins to ... Three different types of catenins seem to exist: alpha, beta, and gamma. Alpha-catenins are proteins of about 100 kDa which are ... In terms of their structure the most significant differences are the absence, in alpha-catenin, of the repeated domain and of ... Vinculin InterPro: IPR000633 Alpha-catenin InterPro: IPR001033 CTNNA1; CTNNA2; CTNNA3; CTNNAL1; VCL; Otto JJ (1990). "Vinculin ...
Wnt/beta-catenin Pathway. The role of Tbx2 in Wnt signaling has yet to be confirmed; however, up-regulation of Tbx2 in the beta ... catenin signaling pathway leads to loss of the adhesion molecule E-cadherin. This returns cells to a mesenchymal state, and ...
beta-catenin signaling cascade". The Journal of Biological Chemistry. 279 (15): 14879-88. doi:10.1074/jbc.M306421200. PMID ... "Wnt-4 activates the canonical beta-catenin-mediated Wnt pathway and binds Frizzled-6 CRD: functional implications of Wnt/beta- ... catenin activity in kidney epithelial cells". Experimental Cell Research. 298 (2): 369-87. doi:10.1016/j.yexcr.2004.04.036. ...
... such as p120-catenin, beta-catenin and alpha-catenin. Catenins, in turn, bind to the actin cytoskeleton. Binding of these ... Although p120 catenin is a potential substrate of PTPmu, others have suggested that the interaction between PTPmu and catenins ... In addition to catenins and cadherins, PTPmu dephosphorylates PIPKIγ90 and nectin-3 (PVRL3) to stabilize E-cadherin-based ... IQGAP1 is a scaffold for Rho family of GTPases, E-cadherin, beta-catenin and other proteins. IQGAP1 binding to Rho GTPases is ...
The flamingo subfamily consists of nonclassic-type cadherins; a subpopulation that does not interact with catenins. The ...
In fact, local synaptic activity changes impact the expression of the cadherin-catenin complexes. An increase in activity at a ... That is, the inter-spine competition for β-catenin determines whether a spine will be matured (increased number of complexes) ... Recent work has implicated the cadherin-catenin complex in a number of different central nervous system processes such as ... This hypothesis suggests that relative levels of cadherin-catenin complexes, which are distributed amongst spines in a local ...
The researchers hypothesized that APC's many β-catenin binding site increase the protein's efficiency at destroying β-catenin, ... interacts with beta-catenin, GSK-3beta and APC and reduces the beta-catenin level". Genes to Cells. 3 (6): 395-403. doi:10.1046 ... interacts with beta-catenin, GSK-3beta and APC and reduces the beta-catenin level". Genes to Cells. 3 (6): 395-403. doi:10.1046 ... Beta-catenin,β-catenin) Human APC genome location and APC gene details page in the UCSC Genome Browser.. ...
They also showed how APC functions - through binding to beta-catenin and stimulating its degradation. Vogelstein and Kinzler ... Su LK, Vogelstein B, Kinzler KW (December 1993). "Association of the APC tumor suppressor protein with catenins". Science. 262 ...
"Identification of the domain of alpha-catenin involved in its association with beta-catenin and plakoglobin (gamma-catenin)". ... Plakoglobin is a member of the catenin protein family and homologous to β-catenin. Plakoglobin is a cytoplasmic component of ... Hearts also exhibited increases in β-catenin signaling. Further investigations on the role of β-catenin and plakoglobin in the ... "A mutation in alpha-catenin disrupts adhesion in clone A cells without perturbing its actin and beta-catenin binding activity ...
Clevers H, Nusse R (June 2012). "Wnt/β-catenin signaling and disease". Cell. 149 (6): 1192-205. doi:10.1016/j.cell.2012.05.012 ... Elevated levels of beta-catenin in both primary and metastases of malignant melanoma have been correlated to improved survival ... Dihlmann, Susanne; von Knebel Doeberitz, Magnus (2005-02-10). "Wnt/β-catenin-pathway as a molecular target for future anti- ... Anagnostou SH, Shepherd PR (December 2008). "Glucose induces an autocrine activation of the Wnt/beta-catenin pathway in ...
Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin ... and ZBTB33 Delta catenin Catenin CTNND2 GRCh38: Ensembl release 89: ENSG00000198561 - Ensembl, May 2017 GRCm38: Ensembl release ... p120, and called catenin delta-1 is a protein that in humans is encoded by the CTNND1 gene. This gene encodes a member of the ... "Entrez Gene: CTNND1 catenin (cadherin-associated protein), delta 1". Schackmann, R. C. J.; Tenhagen, M.; van de Ven, R. A. H.; ...
Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin ... Beta-catenin can also bind to alpha-catenin. Alpha-catenin participates in regulation of actin-containing cytoskeletal ... Loss of E-cadherin expression results in releasing β-catenin into the cytoplasm. Liberated β-catenin molecules may migrate into ... "A truncated beta-catenin disrupts the interaction between E-cadherin and alpha-catenin: a cause of loss of intercellular ...
The Pitx2 gene plays a role in lung adenocarcinoma that is dependent on activating the Wnt/β-catenin signaling pathway. When ... "Identification of a Wnt/Dvl/beta-Catenin --> Pitx2 pathway mediating cell-type-specific proliferation during development". Cell ... analyzing experimental findings from this Wnt/β-catenin signaling pathway, a TCGA dataset showed that Pitx2 had a positive ...
Beta-catenin-like protein 1 is a protein that in humans is encoded by the CTNNBL1 gene. The protein encoded by this gene ... "Entrez Gene: CTNNBL1 catenin, beta like 1". Human CTNNBL1 genome location and CTNNBL1 gene details page in the UCSC Genome ... In addition, the encoded protein contains Armadillo/beta-catenin-like repeats, which have been implicated in protein-protein ...
Yang F, Li X, Sharma M, Sasaki CY, Longo DL, Lim B, Sun Z (March 2002). "Linking beta-catenin to androgen-signaling pathway". ... Masiello D, Chen SY, Xu Y, Verhoeven MC, Choi E, Hollenberg AN, Balk SP (October 2004). "Recruitment of beta-catenin by wild- ... Amir AL, Barua M, McKnight NC, Cheng S, Yuan X, Balk SP (August 2003). "A direct beta-catenin-independent interaction between ... Androgen receptor has been shown to interact with: AKT1, BAG1, Beta-catenin, BRCA1, C-jun, Calmodulin 1, Caveolin 1, CDK9, ...
One study showed that HDAC7 suppresses proliferation and β-catenin activity in chondrocytes. This was shown by knocking out ... Overall, this study demonstrated that HDAC7 once again interacts with β-catenin to keep endothelial cells in a low ... Another study supported the conclusion that HDAC7 and β-catenin associate together by demonstrating that HDAC7 controls ... They found that overexpression of HDAC7 prevented nuclear translocation of β-catenin which then coincided with downregulation ...
GSK-3β, inhibited in some cancer, regulates the stability of β-catenin in cytoplasm and subsequently, cytosolic β-catenin moves ... Ectopic CD97 expression upregulates the expression of N-cadherin and β-catenin in HT1080 fibrosarcoma cells leading to enhanced ... Transgenic expression of a CD97 in mice enhanced levels of nonphosphorylated membrane-bound β-catenin and phosphorylated Akt. ... Wong NA, Pignatelli M (Feb 2002). "Beta-catenin--a linchpin in colorectal carcinogenesis?". The American Journal of Pathology. ...
"Identification of a Wnt/Dvl/beta-Catenin --> Pitx2 pathway mediating cell-type-specific proliferation during development". Cell ...
Armadillo proteins consist of β-catenin, p120-catenin, plakoglobin (γ-catenin), and plakophilins 1-3. In desmosomes, ... Pokutta, Sabine; Weis, William I. (2007). "Structure and mechanism of cadherins and catenins in cell-cell contacts". Annual ...
Arikkath, Jyothi; Reichardt, Louis F. (September 2008). "Cadherins and catenins at synapses: roles in synaptogenesis and ...
... and stabilize beta-catenin. Mutant of presenilin-1 that reduces the ability to stabilize beta-catenin complex leads to ... "Presenilin couples the paired phosphorylation of beta-catenin independent of axin: implications for beta-catenin activation in ... Beta-catenin is coupled by presenilin-1 and undergoes a sequential phosphorylation by two kinase activities. The study also ... Considered as a negative regulator in wnt signaling pathway, presenilin-1 was also found to play a role in beta-catenin ...
... has been found to interact with WNT7B, activating the canonical WNT-CTNNB1/β-catenin signaling pathway. This pathway is ... to numerous cancers because upregulation of the Wnt/β-catenin signaling pathway leads to cytosolic accumulation of β-catenin, ... Wnt/beta-catenin pathway: modulating anticancer immune response. J Hematol Oncol 10, 101 (2017). https://doi.org/10.1186/s13045 ...
When administered to normal mice, it causes nuclear translocation of β-catenin to this region. Modulation of the WNT/β catenin ... It augments the WNT/β catenin pathway to oppose male sex development. In critical gonadal stages, between six and nine weeks ... They help stabilize β-catenin, which activates downstream targets. If both are deficient in XY mice, there is less expression ... Furthermore, it promotes canonical WNT/β catenin signaling. The protein has two cysteine-rich, furin-like domains and one ...
published a research article regarding WNT4 and beta-catenin signaling in medullary kidney fibroblasts and discovered that WNT4 ... Catenin Signaling in Medullary Kidney Myofibroblasts". Journal of the American Society of Nephrology. 24 (9): 1399-1412. doi: ...
... and β-catenin. CK1δ also negatively influences the stability of β-catenin, after its phosphorylation on Ser-45, which permits ... which inhibits the β-catenin destruction complex finally resulting in a higher stability of β-catenin. Here, YAP/Tafazzin (TAZ ... "Mechanism of phosphorylation-dependent binding of APC to beta-catenin and its role in beta-catenin degradation". Molecular Cell ... "Crystal structure of a beta-catenin/axin complex suggests a mechanism for the beta-catenin destruction complex". Genes & ...
α-catenin β-catenin δ-catenin γ-catenin All but α-catenin contain armadillo repeats. Several types of catenins work with N- ... The first two catenins that were identified became known as α-catenin and β-catenin. A-catenin can bind to β-catenin and can ... B-catenin binds the cytoplasmic domain of some cadherins. Additional catenins such as γ-catenin and δ-catenin have been ... Most studies investigating catenin actions focus on α-catenin and β-catenin. β-catenin is particularly interesting as it plays ...
β-Catenin (781 aa) binds E-cadherin and N-cadherin. A second pool of β-catenin is cytoplasmic and coimmunoprecipitates with the ... Ubiquitination of β-catenin is greatly reduced in Wnt-expressing cells. See dapper. Mutations in catenin B1 are associated with ... δ-Catenin-2 (neural plakophilin-related armadillo repeat protein, δ-catenin, neurojungin, CTNND2, 1225 aa) is found in neural ... δ-Catenin-1 (p120, p120ctn, cadherin-associated src substrate, 938 aa) is involved in cell-cell adhesion complexes together ...
Catenin beta; Catnb; Ctnnb; CTNNB1 Beta-catenin (β-catenin) (Armadillo in Drosophila) is a multifunctional protein involved in ... Catenin (cadherin-associated protein), beta 1 (88kD); Catenin beta; Catnb; Ctnnb; CTNNB1 ... Beta-catenin (β-catenin) (Armadillo in Drosophila) is a multifunctional protein involved in two essential cellular events: cell ... 2009). At cell-cell adhesion junctions, β-catenin interacts with type-I cadherins and α-catenin, which in turn associates with ...
Beta-catenin (IPR013284). Short name: Beta-catenin Family relationships *Beta-catenin (IPR013284) *Junction plakoglobin/protein ... Beta-catenin forms a cadherin/beta-catenin/alphaE-catenin complex that can tether the tripartite adhesion complex and regulate ... The beta-catenin structure has been determined [PMID: 9298899, PMID: 11136974]. Beta catenin family proteins contain several ... α-catenin, vinculin, and F-actin in strengthening E-cadherin cell-cell adhesions and mechanosensing.. Cell Adh Migr 7 345-50 ...
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If you know of any papers that use this antibody, please contact us at antibodies [at] alzforum [dot] org for consideration in the References section.. ...
Inhibition of RhoA by p120 catenin.. Anastasiadis PZ1, Moon SY, Thoreson MA, Mariner DJ, Crawford HC, Zheng Y, Reynolds AB. ... The catenin p120 has also been implicated in cadherin clustering through an unknown mechanism. Here we show that p120 ...
J. Liu, J. Stevens, C. A. Rote, H. J. Yost, Y. Hu, K. L. Neufeld, R. L. White, N. Matsunami, Siah-1 mediates a novel β-catenin ... SIP was found to bind to a protein called Ebi, which not only binds to β-catenin, but targets it to an E3 ubiquitinase complex ... Liu et al. report that Siah-1 also interacts with APC, which is required for Siah-1-dependent degradation of β-catenin. This ... Matsuzawa and Reed show that a mammalian protein called Siah-1 lies at the hub of another pathway that destroys β-catenin in ...
... alpha-2-catenin (also called alpha-N-catenin) CTNNA3, alpha-3-catenin (also called alpha-T-catenin) Catenin alpha+Catenin at ... Three alpha-catenin genes are expressed in humans: CTNNA1, alpha-1-catenin (also called alpha-E-catenin) CTNNA2, ... Alpha catenin exhibits significant protein dynamics. The amino acid sequence of alpha-catenin has sequence similarity to that ... It has been observed that when alpha-catenin is not in a molecular complex with beta-catenin, it dimerizes and functions to ...
β-catenin-mediated signaling also can be affected by the association of β-catenin with the tumor suppressor molecule ... Inhibition of β-Catenin-Driven Transactivation by Cadherin Derivatives.. β-Catenin was shown to associate with transcription ... 2C). Similarly, 72% of β-catenin was detergent-insoluble in CHO cells transfected with N-cadherin whereas 64% of β-catenin was ... Competition between the N-cadherin tail and LEF-1 for β-catenin binding. 293 cells were transfected at 1:1 ratio with β-catenin ...
A) Schematic diagram of β-catenin deletion mutants. (B) Interaction of β-catenin deletion mutants with N-cadherin or αN-catenin ... when WT or mutant β-catenin was coexpressed with αN-catenin in HEK293 cells, ΔN and L132A did not bind to αN-catenin, whereas ... 4 A). We first tested the binding of β-catenin mutants to N-cadherin by expressing the epitope-tagged WT or mutant β-catenin ... 4 B). We also tested the two N-terminal domain β-catenin mutants, ΔN and L132A, for their interaction with α-catenin. The ΔN ...
Right panel represents the scheme of chimeric fusion constructs of α-catenin (A), β-catenin (B) or p120 catenin (C) with either ... cadherins cytoplasmic domain binds p120 catenin and beta-catenin, which in turn binds alpha-catenin. To elucidate the roles of ... plasma membrane targeted or untargeted p120 catenin, alpha-catenin and beta-catenin and tested their ability to rescue the loss ... Left panel presents Western blots showing expression of endogenous α-catenin (A), β-catenin (B) and p120ctn (C) and exogenous ...
Cellular β-catenin exists in three different pools: membranous, cytoplasmic and nuclear. In... ... "β-Catenin is essential for embryonic development and required for cell renewal/regeneration in adult life. ... p120 Catenin-associated Fer and Fyn tyrosine kinases regulate β-catenin Tyr-142 phosphorylation and beta-Catenin-alpha-Catenin ... Pokutta S and Weis WI 2000 Structure of the dimerization and beta-catenin-binding region of alpha-catenin. Mol. Cell 5 533-543 ...
It has not been clear how the cytoplasmic protein p120 catenin regulates cell motility. Overexpression of p120 catenin in ... Noren et al. now report that, when in the cytoplasm, p120 catenin binds to Vav2, an exchange factor that activates Cdc42 and ... Noren, N.K., Liu, B.P., Burridge, K., and Kreft, B. (2000) p120 catenin regulates the actin cytoskeleton via Rho family GTPases ... The authors suggest that the distribution of p120 catenin between the cadherin-bound state and the cytoplasmic pool is ...
A new study sheds light on a surprising interaction between FOXO3a and β-catenin in metastatic colorectal cancer, suggesting ... suggesting high β-catenin amounts may enhance the efficacy of BRAF-targeted therapy. Thus, the role of β-catenin in apoptosis ... Figure 1: Concomitant localization of FOXO3a and β-catenin in the nucleus mediate progression and metastasis in colorectal ... β-catenin confers resistance to PI3K and AKT inhibitors and subverts FOXO3a to promote metastasis in colon cancer *Stephan P ...
... have discovered that a single mutation in the beta-catenin gene, which codes a protein known to be deeply involved in a number ... Beta-catenin is an essential protein in the Wnt/beta-catenin signaling pathway, which has been shown in mice to be involved in ... "A single mutation in the beta-catenin gene can lead to infertility." Medical News Today. MediLexicon, Intl., 10 Nov. 2014. Web. ... 2014, November 10). "A single mutation in the beta-catenin gene can lead to infertility." Medical News Today. Retrieved from. ...
Beta-catenin is also involved in the regulation of gene expression as a mediator of the Wnt signaling pathway. The expression ... Beta-catenin is an 88 kDa multifunctional protein playing an essential role in cell-cell adhesion by binding to the ... and intracellular localization of beta-catenin is altered in many types of cancers. ... Clone β-Catenin-1 Beta-catenin is an 88 kDa multifunctional protein playing an essential role in cell-cell adhesion by binding ...
Compare Anti-alpha Catenin Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, ...
Catenins: A family of cytoskeletal proteins that play essential roles in CELL ADHESION at ADHERENS JUNCTIONS by linking ... Catenins. Subscribe to New Research on Catenins A family of cytoskeletal proteins that play essential roles in CELL ADHESION at ... 11/01/1995 - "This study examined the effects of n-6 PUFAs on the expression of catenins in a range of human cancer cells by ... 01/01/1998 - "We found that, although alteration is common in the catenins and E-cadherin, complete loss, as exemplified by E- ...
The structure of the beta-catenin/E-cadherin complex and the molecular basis of diverse ligand recognition by beta-catenin. ... BETA-CATENIN A, C 538 Mus musculus Fragment: ARMADILLO DOMAIN Gene Name(s): Ctnnb1 Catnb ...
X. Q. Gan, J. Y. Wang, Y. Xi, Z. L. Wu, Y. P. Li, and L. Li, "Nuclear Dvl, c-Jun, β-catenin, and TCF form a complex leading to ... Wnt/ß-Catenin: A New Therapeutic Approach to Acute Myeloid Leukemia. Y. Kim, S. Thanendrarajan, and I. G. H. Schmidt-Wolf ... Y. Wang, A. V. Krivtsov, A. U. Sinha et al., "The Wnt/β-catenin pathway is required for the development of leukemia stem cells ... S. L. Holmen, C. R. Zylstra, A. Mukherjee et al., "Essential role of β-catenin in postnatal bone acquisition," Journal of ...
TMEM9-v-ATPase hyperactivates Wnt/β-catenin signalling via lysosomal degradation of adenomatous polyposis coli (APC). Moreover ... Our results reveal the unexpected roles of TMEM9-controlled vesicular acidification in hyperactivating Wnt/β-catenin signalling ... TMEM9 transactivated by β-catenin functions as a positive feedback regulator of Wnt signalling in colorectal cancer. Genetic ... a-d), Activation of Wnt/β-catenin signalling by TMEM9-activated v-ATPase. TMEM9-induced β-catenin stabilization via v-ATPase (a ...
... beta-catenin-TCF7L2 complex, catenin complex, cell cortex, cell junction, cell periphery, cell-cell adherens junction, cell- ... R-SSC-195253. Degradation of beta-catenin by the destruction complex. R-SSC-196299. Beta-catenin phosphorylation cascade. R-SSC ... R-SSC-195253. Degradation of beta-catenin by the destruction complex. R-SSC-196299. Beta-catenin phosphorylation cascade. R-SSC ... Beta-cateninImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a href ...
... Nature. 2015 Jul 9;523(7559):231-5. doi: 10.1038/ ... Molecular analysis of human metastatic melanoma samples revealed a correlation between activation of the WNT/β-catenin ... Using autochthonous mouse melanoma models we identified the mechanism by which tumour-intrinsic active β-catenin signalling ...
Gene ablation and transgenic expression studies strongly support the concept that beta-catenin together with Lef/Tcf factors ... Beta-catenin regulates cell-cell adhesion and transduces signals from many pathways to regulate the transcriptional activities ... Stabilized beta-catenin induces hyperplasia and mammary tumors in mice. Each of the beta-catenin-induced phenotypes is ... Beta-catenin and Tcfs in mammary development and cancer J Mammary Gland Biol Neoplasia. 2003 Apr;8(2):145-58. doi: 10.1023/a: ...
Buy our Recombinant Human delta 1 Catenin protein. Ab114472 is a protein fragment produced in Wheat germ and has been validated ...
Mouse monoclonal gamma Catenin antibody [M354] validated for WB, ICC and tested in Human. Immunogen corresponding to synthetic ... Lanes 1-2 : Anti-gamma Catenin antibody [M354] (ab119772) at 1/1000 dilution. Lanes 3-4 : Anti-gamma Catenin (T916) rabbit ... Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin ... ab119772 at 1/100 dilution staining gamma Catenin in untreated (Top) or pervanadate treated (bottom) A431 cells. Detection ...
Compare catenin alpha 1 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and ... catenin alpha 1 ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody-based tool for ... Mouse/Human/Rat Alpha 1+2 Catenin ELISA Kit (Cell-Based ELISA) ... Your search returned 152 catenin alpha 1 ELISA ELISA Kit across ...
Stablized β-catenin is translocated to the nucleus via Rac1 and other factors, where it binds to LEF/TCF transcription factors ... Valenta T, Hausmann G, Basler K (2012) The many faces and functions of β-catenin. EMBO J.. 31(12), 2714-36. ... MacDonald BT, Tamai K, He X (2009) Wnt/beta-catenin signaling: components, mechanisms, and diseases. Dev. Cell. 17(1), 9-26. ... In the absence of Wnt-signal (Off-state), β-catenin, an integral E-cadherin cell-cell adhesion adaptor protein and ...
  • Secondly, β-catenin participates in the Wnt signaling pathway as a downstream target. (wikipedia.org)
  • While the pathway is very detailed and not completely understood, in general, when Wnt is not present, GSK-3B (a member of the pathway) is able to phosphorylate β-catenin as a result of a complex formation that includes β-catenin, AXIN1, AXIN2, APC (a tumor suppressor gene product), CSNK1A1, and GSK3B. (wikipedia.org)
  • A second pool of β-catenin is cytoplasmic and coimmunoprecipitates with the adenomatous polyposis coli (APC) tumour suppressor protein, interacts with Tcf and Lef transcription factors, and is an essential member of the Wingless wnt signal transduction pathway. (oxfordreference.com)
  • β-Catenin/armadillo (Arm) was initially identified as a segment polarity protein in Drosophila in the early 1980s, and later recognized as a key downstream effector of the Wnt pathway. (springer.com)
  • Kikuchi A. Regulation of beta-catenin signaling in the Wnt pathway. (ebi.ac.uk)
  • Matsuzawa and Reed show that a mammalian protein called Siah-1 lies at the hub of another pathway that destroys β-catenin in response to DNA damaging agents and the activation of the tumor suppressor protein p53. (sciencemag.org)
  • This alternate β-catenin destruction pathway may link p53 to cell-cycle arrest. (sciencemag.org)
  • S. Matsuzawa, J.C. Reed, Siah-1, SIP, and Ebi collaborate in a novel pathway for β-catenin degradation linked to p53 responses. (sciencemag.org)
  • J. Liu, J. Stevens, C. A. Rote, H. J. Yost, Y. Hu, K. L. Neufeld, R. L. White, N. Matsunami, Siah-1 mediates a novel β-catenin degradation pathway linking p53 to the adenomatous polyposis coli protein. (sciencemag.org)
  • It was shown that the Drosophila segment polarity gene product armadillo, a homologue of vertebrate plakoglobin and β-catenin, is part of a signaling pathway driven by the secreted molecule wingless (wg) ( 10 ). (pnas.org)
  • Arend RC, Londoño-Joshi AI, Straughn JM and Buchsbaum DJ 2013 The Wnt/β-catenin pathway in ovarian cancer: a review. (springer.com)
  • A new study sheds light on a surprising interaction between FOXO3a and β-catenin in metastatic colorectal cancer, suggesting new therapeutic avenues for agents targeting the PI3K-AKT pathway (pages 892-901 ). (nature.com)
  • Beta-catenin is an essential protein in the Wnt/beta-catenin signaling pathway, which has been shown in mice to be involved in the development and maintenance of most, if not all organs, throughout their lives. (medicalnewstoday.com)
  • The research has also elucidated a new function for the Wnt/beta-catenin pathway, which plays a role in regulating cell growth as well as cell-cell communication. (medicalnewstoday.com)
  • This finding came as a major surprise because we were expecting to see effects on many organs, since the Wnt/beta-catenin pathway is so ubiquitous. (medicalnewstoday.com)
  • Beta-catenin is also involved in the regulation of gene expression as a mediator of the Wnt signaling pathway. (agilent.com)
  • The Wnt/ β -catenin pathway is required for the development of leukemia stem cells in AML," Science , vol. 327, no. 5973, pp. 1650-1653, 2010. (hindawi.com)
  • Axin-mediated CKI phosphorylation of β -catenin at Ser 45: a molecular switch for the Wnt pathway," Genes and Development , vol. 16, no. 9, pp. 1066-1076, 2002. (hindawi.com)
  • Molecular analysis of human metastatic melanoma samples revealed a correlation between activation of the WNT/β-catenin signalling pathway and absence of a T-cell gene expression signature. (nih.gov)
  • The conserved Wnt/β-Catenin pathway regulates stem cell pluripotency and cell fate decisions during development. (cellsignal.com)
  • In the absence of Wnt-signal (Off-state), β-catenin, an integral E-cadherin cell-cell adhesion adaptor protein and transcriptional co-regulator, is targeted by coordinated phosphorylation by CK1 and the APC/Axin/GSK-3β-complex leading to its ubiquitination and proteasomal degradation through the β-TrCP/Skp pathway. (cellsignal.com)
  • Curcumin affects β-catenin pathway in HSCs and might suggest a possible new explanation for the effects of curcumin on HSC activation and liver fibrosis. (greenmedinfo.com)
  • This was accompanied by accumulation of stabilized active nonphosphorylated β-catenin, strongly suggesting aberrant activation of the WNT/β-catenin signaling pathway in these tumors. (sigmaaldrich.com)
  • These results suggest that elevated Aβ levels in AD brains may impair neuron induction from GPCs by interfering with the β-catenin signaling pathway. (jneurosci.org)
  • β-catenin has received much attention for its role as the cadherin counter-receptor in cell-cell adhesion and as a player in the Wnt signaling pathway in development and cancer, but there is also significant data linking the protein to synaptic activity and plasticity, at least in vitro (for review, see Salinas and Price, 2005 ). (alzforum.org)
  • The Stowers Institute's Pourquié Lab has demonstrated the importance of Beta-catenin, a key component of the Wnt-signaling pathway in the process of somite formation. (innovations-report.com)
  • In this work, the Pourquié team tested the importance of Beta-catenin, a protein that functions as the principal mediator of the Wnt-signaling pathway, in the process of somite formation. (innovations-report.com)
  • Wnt/β‐catenin signalling pathway in vertebrates. (els.net)
  • Of the many growth factors involved in these events, Wnts are particularly interesting regulators, because a key component of their signaling pathway, β-catenin, also functions as a component of the cadherin complex, which controls cell-cell adhesion and influences cell migration. (sciencemag.org)
  • The central player is β-catenin, which is a transcription cofactor with T cell factor/lymphoid enhancer factor TCF/LEF in the Wnt pathway ( 2 ) and a structural adaptor protein linking cadherins to the actin cytoskeleton in cell-cell adhesion ( 5 ). (sciencemag.org)
  • Wnt signaling, proceeding through Frizzled and Arrow-LRP-5/6, activates Dishevelled (Dsh), which results in uncoupling β-catenin from the degradation pathway and its entry into the nucleus, where it interacts with TCF/LEF to control transcription. (sciencemag.org)
  • The canonical Wnt pathway mediator, β-catenin, controls the expression of Atoh1 . (jneurosci.org)
  • Aberrant activation of the β-catenin signaling pathway has been implicated in several malignancies, including breast carcinoma. (wiley.com)
  • The authors examined the expression of β-catenin, key members of its signaling pathway, and p53 in a large cohort of breast tumors. (wiley.com)
  • β-Catenin function is essential for the canonical arm of the Wnt signaling pathway (reviewed by Willert and Nusse, 1998 ). (biologists.org)
  • These data indicate that gal-3 is a novel binding partner for β-catenin involved in the regulation of Wnt/β-catenin signaling pathway. (aacrjournals.org)
  • β-Catenin is a downstream component of the Wnt signaling pathway. (aacrjournals.org)
  • We have previously shown that gal-3 overexpression regulates changes in the expression levels of cell cycle regulators, including cyclin D 1 (13) , and because cyclin D 1 has been reported to be targeted within the Wnt pathway (7) , we questioned the possible association between gal-3 and β-catenin for molecular shuttling to the nucleus of cancer cells. (aacrjournals.org)
  • Shikonin inhibits adipogenesis by modulation of the WNT/β-catenin pathway. (biomedsearch.com)
  • Among the genes located in the WNT/β-catenin pathway, the levels of WNT10B and DVL2 were significantly up-regulated, whereas the level of AXIN was down-regulated by shikonin treatment. (biomedsearch.com)
  • SIGNIFICANCE: This study clearly shows that shikonin inhibits adipogenesis by the modulation of WNT/β-catenin pathway in vitro, and also suggests that WNT/β-catenin pathway can be used as a therapeutic target for obesity and related diseases using a natural compound like shikonin, even though the in vivo effects of shikonin and its clinical significance remain to be elucidated. (biomedsearch.com)
  • Upon binding, Wnt signaling triggers different downstream responses dependent on β-catenin (also known as the canonical pathway), JNK, or PKC (also known as noncanonical pathways). (rupress.org)
  • The canonical pathway instead is characterized by the inhibition of glycogen synthase kinase 3 β (GSK3-β)-dependent phosphorylation of β-catenin, thus blocking its degradation and resulting in its nuclear translocation and activation of β-catenin/TCF-responsive genes. (rupress.org)
  • The role of E- cadherin in the malfunction of cell-cell adhesion observed in colorectal cancer, and in the beta-catenin degradation system after mutations that affect the wnt-pathway, belong to the most studied examples (2-4). (redorbit.com)
  • Lpcat3KD 3T3L1 pre-adipocytes were treated with different concentration of Wnt/-catenin pathway inhibitors and then underwent differentiation. (selleckchem.com)
  • β-catenin is a critical component of the Wnt signaling pathway, which is itself important in embryogenesis, stem cell differentiation and tumorigenesis. (fluidigm.com)
  • Click on one of the boxes below to see the factors involved in the Wnt/Ca 2+ signaling pathway or other beta-Catenin-independent Wnt signaling pathways. (rndsystems.com)
  • Note: The beta-Catenin-independent Wnt/PCP pathway is listed in the pathway index as a separate pathway. (rndsystems.com)
  • The mutations and deregulation of Wnt signaling pathway occur commonly in human cancer and cause the aberrant activation of β-catenin and β-catenin-dependent transcription, thus contributing to cancer development and progression. (frontiersin.org)
  • Similarly, the NF-κB pathway either positively or negatively regulates Wnt/β-catenin signaling. (frontiersin.org)
  • Wnt/β-catenin pathway components modulate inflammatory and immune responses via the interaction with NF-κB ( 5 ). (frontiersin.org)
  • Reciprocally, NF-κB also influences the activity of Wnt/β-catenin signaling pathway. (frontiersin.org)
  • Members of the Wnt/β-catenin pathway also serve as potential therapeutic targets for many types of cancer ( 9 ). (frontiersin.org)
  • β-Catenin is a central mediator of Wnt signaling pathway, components of which have been implicated in B cell development and function. (jimmunol.org)
  • LEF-1 was originally identified in lymphocytes, and later, LEF-1/TCF family of transcription factors were shown to be the major effectors of the canonical Wnt signaling pathway with β-catenin as a cofactor. (jimmunol.org)
  • Here, we determined the expression patterns of Wnt signaling pathway molecules by quantitative reverse transcription polymerase chain reaction, western blot, and immunohistochemistry and found that β-catenin signaling was suppressed by Wnt5a. (medsci.org)
  • Mechanistic analysis revealed that MASTL expression facilitates colon cancer progression by promoting the β-catenin/Wnt signaling, the key signaling pathway implicated in colon carcinogenesis, and up-regulating anti-apoptotic proteins, Bcl-xL and Survivin. (unboundmedicine.com)
  • The Wnt/β-catenin signaling pathway is known to be involved in the differentiation process of hNPCs. (inria.fr)
  • In this paper, we present a computational modeling study on the dynamics of the Wnt/β-catenin signaling pathway in RVM cells, a common cell model of hNPCs. (inria.fr)
  • To this end, we present a model of RVM cell populations that combines the Wnt/β-catenin signaling pathway with cell cycle control. (inria.fr)
  • These let us conclude that crucial mechanisms of noise reduction in the Wnt/β-catenin signaling pathway may still be unknown. (inria.fr)
  • The Wnt/β-catenin signaling pathway is important for multiple developmental processes and tissue maintenance in adults. (uva.nl)
  • We furthermore simulated the effect of APC inactivating mutations, yielding a stabilization of β-catenin levels comparable to the Wnt-pathway activities observed in colorectal and breast cancer. (uva.nl)
  • β-catenin is the key component of the canonical Wnt pathway and plays a crucial role in a multitude of developmental and homeostatic processes. (mcponline.org)
  • β-catenin is the key effector molecule of the canonical Wnt pathway and it exerts two crucial roles within the cell. (mcponline.org)
  • The Wnt/Beta-catenin pathway mediates the transcription of proteins important for maintenance and growth of hematopoietic stem cells. (wikipathways.org)
  • To exploit the activated pathways and develop new strategies to overcome trastuzumab resistance, we investigated MKN45 and MKN45/R cells via label-free quantitative proteomics, and found pathways that were altered significantly in MKN45/R cells, with the Wnt/β-catenin pathway being the most significant. (mdpi.com)
  • We further confirmed the activation of this pathway by detecting its key molecules in MKN45/R and NCI N87/R cells via Western blot, in which Wnt3A, FZD6, and CTNNB1 increased, whereas GSK-3β decreased, manifesting the activation of the Wnt/β-catenin pathway. (mdpi.com)
  • Correspondingly, inhibition of Wnt/β-catenin pathway by ICG-001, a specific Wnt/β-catenin inhibitor, preferentially reduced proliferation and invasion of trastuzumab-resistant cells and reversed EMT. (mdpi.com)
  • Taken together, our study demonstrates that the Wnt/β-catenin pathway mediates trastuzumab resistance, and the combination of Wnt/β-catenin inhibitors with trastuzumab may be an effective treatment option. (mdpi.com)
  • Beta-catenin is a component of the adherens junction, a multiprotein complex which supports Ca 2+ -dependent cell-to-cell contact which in itself is critical for adhesion, signal transmission and for anchoring the actin cytoskeleton.Beta-catenin's role is as a transcription effector of the wnt-signalling pathway. (leicabiosystems.com)
  • Immunohistochemistry is the best way to demonstrate nuclear expression of beta-catenin and wnt-pathway activation. (leicabiosystems.com)
  • However, the mechanisms underlying EGF-β-catenin pathway-induced EMT of glioblastoma multiforme (GBM) have not been reported previously. (dovepress.com)
  • In the present study, immunohistochemistry, reverse transcription polymerase chain reaction, and Western blot were applied to investigate the effect of EGF-β-catenin pathway on EMT of GBM. (dovepress.com)
  • In conclusion, β-catenin-EMT pathway induced by EGF is important for GBM progression by the PI3K/Akt pathways. (dovepress.com)
  • Inhibition of β-catenin leads to suppression of EGF pathway-induced EMT, which provides a new way to treat GBM patients. (dovepress.com)
  • Among the molecular signaling pathways implicated in the pathogenesis of HCC, the Wnt/β-catenin signaling pathway is one of the most frequently activated. (dovepress.com)
  • In this article, we review the role of the β-catenin pathway in hepatocarcinogenesis and progression from chronic inflammation to HCC, the novel potential treatments targeting the pathway and its prognostic role in HCC patients, as well as the imaging features of HCC and their association with aberrant activation of the pathway. (dovepress.com)
  • 1 In the absence of a Wnt ligand, the central mediator of this signaling pathway, β-catenin, is constitutively phosphorylated by a destruction complex (DC) consisting of GSK3β, CK1, Axin and APC, priming it for subsequent degradation by the proteasome. (haematologica.org)
  • δ-Catenin-2 (neural plakophilin-related armadillo repeat protein, δ-catenin, neurojungin, CTNND2, 1225 aa) is found in neural tissues and, together with kaiso and myogenic transcription factors, regulates synapse-specific transcription of rapsyn. (oxfordreference.com)
  • Regulation of Wnt/beta-catenin signaling by protein kinases. (springer.com)
  • Zhurinsky J, Shtutman M. Ben-Ze'ev A. Plakoglobin and beta-catenin: protein interactions, regulation and biological roles. (springer.com)
  • This entry represents the beta catenins and homologues, plakoglobin and the Drosophila Armadillo protein [ PMID: 2261639 ], which are implicated in cell adhesion and Wnt signalling [ PMID: 9425166 ]. (ebi.ac.uk)
  • Beta catenin family proteins contain several ARM repeats, sequences of approximately 50 amino acids involved in protein-protein interactions. (ebi.ac.uk)
  • Alpha-catenin functions as the primary protein link between cadherins and the actin cytoskeleton. (wikipedia.org)
  • It has been observed that when alpha-catenin is not in a molecular complex with beta-catenin, it dimerizes and functions to regulate actin filament assembly[citation needed], possibly by competing with Arp2/3 protein. (wikipedia.org)
  • Alpha catenin exhibits significant protein dynamics. (wikipedia.org)
  • Regulating the cytosolic concentration of the protein β-catenin is an important cell proliferation control mechanism. (sciencemag.org)
  • The cytoplasmic concentration of β-catenin remains low through an interaction with a protein complex consisting of adenomatous polyposis coli (APC), Axin, protein phosphatase 2A, and glycogen synthase kinase 3β (GSK3β). (sciencemag.org)
  • SIP was found to bind to a protein called Ebi, which not only binds to β-catenin, but targets it to an E3 ubiquitinase complex and hence, leads to the proteolysis of β-catenin. (sciencemag.org)
  • N-cadherin directed β-catenin into adherens junctions, and the chimeric protein induced diffuse distribution of β-catenin along the membrane whereas the cytoplasmic domain of N-cadherin colocalized with β-catenin in the nucleus. (pnas.org)
  • It has not been clear how the cytoplasmic protein p120 catenin regulates cell motility. (sciencemag.org)
  • In this context, tumor cells with low nuclear β-catenin undergo apoptosis (partially responding carcinoma), whereas those with high nuclear protein levels of both β-catenin and FOXO3a become resistant to induction of apoptosis. (nature.com)
  • Scientists from the RIKEN BioResource Center in Tsukuba, Japan, have discovered that a single mutation in the beta-catenin gene, which codes a protein known to be deeply involved in a number of developmental and homeostatic processes, can lead to infertility not through a disruption of the production of egg or sperm cells, but rather by leading to abnormalities in the morphology of the sexual organs, making natural reproduction impossible. (medicalnewstoday.com)
  • The BRC research group, in a study published in Scientific Reports , developed a mouse with single mutation to the beta-catenin gene, with the goal to discover so far unrevealed functions of beta-catenin, a protein that is well conserved through evolution. (medicalnewstoday.com)
  • Beta-catenin is an 88 kDa multifunctional protein playing an essential role in cell-cell adhesion by binding to the transmembrane protein, cadherin. (agilent.com)
  • Recombinant C-terminal ß-catenin fusion protein (6). (agilent.com)
  • In Western blotting of human epithelial A431 cells the antibody labels a band corresponding to human ß-catenin protein. (agilent.com)
  • Using Ai34D mice that conditionally expressed synaptophysin-tdTomato, a pre-synaptic vesicle-related protein, they found that β-catenin promotes maturation of synaptophysinpuncta in pre-synaptic loci. (eurekalert.org)
  • Through co-immunoprecipitation and mass spectrometry experiments, researchers identified p140Cap, a protein known to regulate exocytosis, as a novel binding partner of β-catenin. (eurekalert.org)
  • I n the absence of a Wnt signal, ß -catenin is degraded by a complex of proteins including Axin, APC, the Ser / Thr kinases GSK-3 and CK1, protein phosphatase 2A (PP2A), and the E3-ubiquitin ligase b -TrCP. (stanford.edu)
  • Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. (thermofisher.com)
  • Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. (thermofisher.com)
  • The E-cadherin/catenin complex is a substrate of receptor tyrosine kinases e.g. c-erbB-2, c-met, EGFR, of non-receptor tyrosine kinases e.g. src, of receptor protein tyrosine phosphatases and of MUC-1 (episialin). (uninet.edu)
  • β-Catenin protein is a member of the 'armadillo' superfamily and was originally described as an element of the E-cadherin/catenin complex. (uninet.edu)
  • In normal cells, β-catenin is associated not only with cadherins but also with the APC (adenomatous polyposis coli) multi-protein complex. (uninet.edu)
  • In either case, the results supported a crucial role for β-catenin in fear learning: stabilization of the protein enhanced the fear behavior, while deletion impaired it. (alzforum.org)
  • Deletion of β-catenin after training did not erode long-term memory, suggesting again that protein is required at the point of memory consolidation, but not for long-term maintenance. (alzforum.org)
  • They showed that a newly identified Beta-catenin protein gradient in the PSM is critical in regulating mesoderm maturation. (innovations-report.com)
  • Real-time imaging experiments also demonstrated that, conversely, the segmentation clock is not caused by graded levels of Beta-catenin protein. (innovations-report.com)
  • We were able to demonstrate that increasing Beta-catenin protein levels dramatically alters PSM maturation," said Alexander Aulehla, M.D., Senior Research Associate and first author on the paper. (innovations-report.com)
  • Posttranslational stabilization of β-catenin, leading to elevated protein levels and constitutive gene activation, has been proposed as an important step in oncogenesis. (aacrjournals.org)
  • β-Catenin is targeted for ubiquitination and degradation in the 26 S proteosome by paired phosphorylation through the serine/threonine kinases casein kinase I (CKI) and glycogen synthase-3β (GSK-3β) ( 7 ) bound to a scaffolding complex of axin and adenomatous polyposis coli (APC) protein ( 2 , 8 ). (sciencemag.org)
  • Here, we revisit and review the functions, phylogenetic classifications and co-evolution of the cadherin and catenin protein families. (ovid.com)
  • β-catenin is a multi functional protein that is involved in cell-cell adhesion and cell signaling. (oregonstate.edu)
  • Protein analysis by Western blotting showed massive accumulation of mutant β-catenin. (oregonstate.edu)
  • β-Catenin is a 92kDa protein that binds to the cytoplasmic tail of E-Cadherin. (fishersci.com)
  • β-Catenin was found to co-immunoprecipitate with the APC tumor suppressor protein in human colorectal tumor cell lines, as well as in human kidney 293 cells. (fishersci.com)
  • In the absence of Wnt ligand, cytoplasmic pools ofβ -catenin are highly unstable because of multiple phosphorylations in the N terminus of the protein that target the protein for degradation. (biologists.org)
  • Recombinant chicken beta Catenin fused to maltose binding protein. (fishersci.com)
  • Galectin-3 (gal-3), a pleiotrophic protein, is an important regulator of tumor metastasis, which like β-catenin shuttles between the nucleus and the cytosol in a phosphorylation-dependent manner. (aacrjournals.org)
  • Shikonin-induced reductions of the major transcription factors of adipogenesis including peroxisome proliferator-activated receptor γ and CCAAT/enhancer binding protein α, and lipid metabolizing enzymes including fatty acid binding protein 4 and lipoprotein lipase, as well as intracellular fat accumulation, were all significantly recovered by siRNA-mediated knockdown of β-catenin. (biomedsearch.com)
  • beta-catenin is an intracellular protein associated with the actin cytoskeleton of a cell. (redorbit.com)
  • In this process there are multiple protein-complexes involved which interact with beta-catenin and E-cadherin. (redorbit.com)
  • PRI-724 (IC50=150 nM) and ICG-001 (IC50=3 μM) inhibit the recruiting of β-catenin with its coactivator element-binding protein(CBP) in nucleus. (selleckchem.com)
  • Clone D10A8 recognizes endogenous levels of total β-catenin protein. (fluidigm.com)
  • A key component of the activity is Kaiso, a protein with POZ and zinc-finger domains that is known to associate with p120 catenin. (sigmaaldrich.com)
  • Engagement of Frizzled receptors by Wnt ligand results in the accumulation of β-catenin protein. (jimmunol.org)
  • In the absence of Wnt signal, β-catenin is found in a complex with scaffolding protein Axin, the tumor suppressor adenomatous polyposis coli, GSK3β, and β-TrCP. (jimmunol.org)
  • Gamma Catenin is a common junctional plaque protein. (genetex.com)
  • This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. (genetex.com)
  • α-catenin was initially described as an E-cadherin associated protein, and since has been shown to associate with other members of the cadherin family, such as N-cadherin and P-cadherin. (scbt.com)
  • β-catenin has also been found in complexes with the tumor suppressor protein APC. (scbt.com)
  • The highly tyrosine phosphorylated adherens junction protein p120 catenin (p120) belongs to a subfamily of Armadillo-domain proteins. (biologists.org)
  • Knockdown of β-catenin by RNAi resulted in significant reduction of ERα mRNA and/or protein levels in MCF-7, T-47D, and BT-474 breast cancer cells and in significant reduction of estradiol-induced expression of the ERα target genes pS2 and GREB1. (mdpi.com)
  • It is concluded that β-catenin is a positive regulator of ERα mRNA and protein expression. (mdpi.com)
  • Expression of the gof-β-catenin mutant protein in the epidermis severely impaired eyelid morphogenesis at E15.5, E17.5, and E18.5. (molvis.org)
  • Expression of the gof-β-catenin mutant protein in basal epithelial cells disrupts eyelid and cornea morphogenesis during mouse embryonic development due to the perturbation of cell proliferation and differentiation of the epithelium and the neural crest-derived mesenchyme. (molvis.org)
  • At least a portion of the electrophoretic band migrating at the position of γ-catenin consists of plakoglobin, a desmosomal and zonula adherens protein that has high sequence similarity to β-catenin and armadillo . (biologists.org)
  • The inhibition of Wnt leads to protein degradation through Beta-Catenin activation by the Axin/APC/CK1/GSK3B protein complex. (wikipathways.org)
  • In metazoan adherens junctions, β-catenin links the cytoplasmic tail of classical cadherins to the F-actin-binding protein α-catenin. (osti.gov)
  • Here, we identified that β-catenin mRNA and protein levels were up-regulated in GBM tissues and four kinds of glioblastoma cell lines, including T98G, A172, U87, and U251 cells, compared with normal brain tissue and astrocytes. (dovepress.com)
  • Using Ai34D mice that conditionally expressed synaptophysin-tdTomato, a pre-synaptic vesicle-related protein, they found that β-catenin promotes maturation of synaptophysin puncta pre-synaptic loci. (medicalxpress.com)
  • Using autochthonous mouse melanoma models we identified the mechanism by which tumour-intrinsic active β-catenin signalling results in T-cell exclusion and resistance to anti-PD-L1/anti-CTLA-4 monoclonal antibody therapy. (nih.gov)
  • Each Catenin alpha Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • The following antibody was used in this experiment: beta Catenin Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-16762, RRID AB_10980908. (thermofisher.com)
  • This antibody reacts with the C-terminal portion of beta-Catenin. (thermofisher.com)
  • The following product was used in this experiment: Beta-catenin Antibody (UMAB15), UltraMAB™ Mouse Monoclonal from Thermo Fisher Scientific, catalog # UM500015CF. (thermofisher.com)
  • Antibody specific for phosphorylated β-catenin showed that the accumulated D32G and D32N β-catenin proteins were strongly phosphorylated both in vivo and in vitro, whereas D32Y β-catenin exhibited significantly attenuated phosphorylation in vivo. (oregonstate.edu)
  • ICC/IF analysis of HeLa cell using GTX32619 gamma Catenin antibody. (genetex.com)
  • WB analysis of various samples using GTX32619 gamma Catenin antibody. (genetex.com)
  • B-catenin binds the cytoplasmic domain of some cadherins. (wikipedia.org)
  • The name "catenin" was originally selected ('catena' means 'chain' in Latin) because it was suspected that catenins might link cadherins to the cytoskeleton. (wikipedia.org)
  • Several types of catenins work with N-cadherins to play an important role in learning and memory (For full article, see Cadherin-catenin complex in learning and memory). (wikipedia.org)
  • Catenins play roles in cellular organization and polarity long before the development and incorporation of Wnt signaling pathways and cadherins. (wikipedia.org)
  • The primary mechanical role of catenins is connecting cadherins to actin filaments, specifically in these adhesion junctions of epithelial cells. (wikipedia.org)
  • 2009 ). At cell-cell adhesion junctions, β-catenin interacts with type-I cadherins and α-catenin, which in turn associates with the actin cytoskeleton. (springer.com)
  • β-Catenin binds directly to the cytoplasmic tail of classical cadherins. (wikipedia.org)
  • Whereas the extracellular domain of cadherins provides the direct intercellular link between apposing cells, strong adhesion by cadherins requires the dynamic intracellular connection to the actin cytoskeleton through β- and α-catenins ( 17 , 18 ). (pnas.org)
  • Accordingly, impairing the adhesive activity of cadherins by deletion of β-catenin or N-cadherin reduces the number of reserve pool synaptic vesicles at the presynaptic terminal, resulting in enhanced synaptic depression during repetitive stimulation ( 21 , 22 ). (pnas.org)
  • Because cadherins mediate homophilic cell adhesion, it is assumed that any defects in the cadherin-catenin complex are exerted on both sides of the intercellular junction. (pnas.org)
  • Overexpression of p120 catenin in fibroblasts modulates the actin cytoskeleton and promotes cell motility, yet binding to the cytoplasmic domain of coexpresssed cadherins, cell surface adhesion proteins, inhibits this effect. (sciencemag.org)
  • Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. (abcam.com)
  • Catenins have been identified as cytoplasmic anchorage proteins for cadherins. (uninet.edu)
  • In the absence of Wnt signals (left), newly synthesized β‐catenin either binds to cadherins at the plasma membrane, or is phosphorylated by CK1 then GSK‐3, probably in a degradation complex containing Axin and APC, leading to its being ubiquitinated and degraded by the proteosome. (els.net)
  • Heasman J, Crawford A and Goldstone K (1994) Overexpression of cadherins and underexpression of β‐catenin inhibit dorsal mesoderm induction in early Xenopus embryos. (els.net)
  • In the absence of Wnt signaling, the level of β-catenin is kept low through degradation of (cytoplasmic) β-catenin that is in excess of binding sites, such as cadherins at the plasma membrane (see below). (sciencemag.org)
  • Even genomes of ancestral metazoan species such as cnidarians and placozoans encode a limited number of distinct cadherins and catenins, emphasizing the conservation and functional importance of these gene families. (ovid.com)
  • The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions (zonula adherens). (fishersci.com)
  • On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. (cellsignal.com)
  • Research studies have demonstrated that loss of E-cadherin and α-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1).Research studies also suggest that, rather than acting as a static link between cadherins and actin, α-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex (2,3). (cellsignal.com)
  • The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. (cellsignal.com)
  • Both plakoglobin and β -catenin bind to cadherins to establish cell adhesion. (stanford.edu)
  • E- cadherins bind to beta-catenin to form a complex which can interact both with neighboring cells to form bonds, and with the cytoskeleton of the cell. (redorbit.com)
  • The alpha- catenin and beta-catenin then form a complex to link the actin filaments of the cytoskeleton and the E-cadherins. (redorbit.com)
  • Firstly, it functions in cell adhesion at the plasma membrane where it connects cadherins via α-catenin to the cytoskeleton ( 1 ) and secondly, it mediates the expression of genes controlled by Wnt-responsive elements as a transcriptional co-activator ( 2 , 3 ). (mcponline.org)
  • The catenins are polypeptides that bind to the conserved cytoplasmic tail of cadherins and are required for cadherin function. (biologists.org)
  • α-Catenin is related to vinculin and seems to be required for the interaction of cadherins with the actin cytoskeleton. (biologists.org)
  • Cadherins are thought to connect to the actin cytoskeleton indirectly through the proteins α-catenin and β-catenin. (rupress.org)
  • α-catenin β-catenin δ-catenin γ-catenin All but α-catenin contain armadillo repeats. (wikipedia.org)
  • The armadillo homologs beta-catenin and plakoglobin are differentially expressed during early development of Xenopus laevis. (ebi.ac.uk)
  • Three-dimensional structure of the armadillo repeat region of beta-catenin. (ebi.ac.uk)
  • In both Drosophila and Xenopus , it was demonstrated that the signaling activity of armadillo/β-catenin is independent of cadherin-based adhesion ( 15 , 16 ). (pnas.org)
  • On the other hand, this signaling is strongly affected by the levels of cadherin because overexpression of cadherin mimics the wg phenotype in Drosophila ( 16 ) and blocks β-catenin signaling in Xenopus ( 15 ), suggesting that cadherin may be a negative regulator of armadillo/β-catenin signaling. (pnas.org)
  • P120 catenin (p120 CTN ) is a member of a large subfamily of Armadillo proteins. (uninet.edu)
  • Its cytoplasmic tail interacts with the armadillo catenins, p120 and β-catenin. (ovid.com)
  • Further, α-catenin links the cadherin/armadillo catenin complex to the actin filament network. (ovid.com)
  • β -catenin (armadillo in Drosophila) is the key mediator of the Wnt signal. (stanford.edu)
  • Vertebrates have two Armadillo/ β -catenin homologs, β -catenin and plakoglobin (also called gamma-catenin). (stanford.edu)
  • β-Catenin is homologous to armadillo , a segment polarity gene in Drosophila that participates in developmental signaling. (biologists.org)
  • The sequences of the predicted HMP-1, HMP-2, and HMR-1 proteins are related to the cell adhesion proteins α-catenin, β-catenin/Armadillo, and classical cadherin, respectively. (rupress.org)
  • Stabilization of β-catenin can occur through mutation to highly conserved amino acids encoded in exon 3 of the β-catenin gene ( CTNNB1 ). (aacrjournals.org)
  • The constitutively expressed CTNNB1 (commonly and hereafter referred to as β-catenin) is degraded by a destruction complex, composed of amongst others AXIN1 and GSK3. (uva.nl)
  • In vivo, conditional knock-out of ß-catenin gene (Ctnnb1) did not have significant effects on corneal morphogenesis or homeostasis, but abolished OSSN formation in Dox-treated Krt12rtTA/rtTA/tetO-FGF-7/tetO-Cre/Ctnnb1lox(E2-6)/lox(E2-6) mice. (novapublishers.com)
  • Compound mutant mice ( Ctnnb1 GOFOSE , gof of β-catenin in the epidermis and the ocular surface epithelium) were generated by time-mating keratin 5-promoter-Cre recombinase ( Krt5-Cre ) and Ctnnb1 fE3/WT (floxed exon 3 of Ctnnb1 ) mice. (molvis.org)
  • In the present study, 31 brain metastases that originated from primary lung carcinomas were analyzed regarding over expression of Dishevelled-1 (DVL1), Dishevelled-3 (DVL3), E-cadherin (CDH1) and beta-catenin (CTNNB1). (mdpi.com)
  • Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells. (wikipedia.org)
  • It has been reported that the actin binding proteins vinculin and alpha-actinin can bind to alpha-catenin. (wikipedia.org)
  • To elucidate the roles of the cadherin-associated proteins in regulating microtubule dynamics, we prepared GFP-tagged, plasma membrane targeted or untargeted p120 catenin, alpha-catenin and beta-catenin and tested their ability to rescue the loss of microtubule mass caused by centrosomal removal in the poorly adhesive cell line CHO-K1. (nih.gov)
  • Left panel presents Western blots showing expression of endogenous α-catenin (A), β-catenin (B) and p120ctn (C) and exogenous transfected EGFP-derivatives of corresponding proteins and their membrane-targeted IL2R fusion constructs. (nih.gov)
  • For more than a decade, cell, developmental and cancer investigators have brought about a wide interest in the biology of catenin proteins, an attraction being their varied functions within differing cellular compartments. (nih.gov)
  • We observed the changes of the expression of exchange proteins directly activated by cAMP-1 (Epac1) and p120 catenin (p120), the quantities of macrophages and endothelial cells, vascular endothelial permeability, and mechanical withdrawal threshold (MWT). (hindawi.com)
  • To investigate the mechanisms underlying this difference, we compared β-catenin signaling proteins in GPCs from AD versus HC subjects and studied the effect of amyloid β peptide (Aβ, a hallmark of AD pathology) on GPCs. (jneurosci.org)
  • we found that Aβ treatment impaired the ability of GPCs from HC subjects to generate new neurons and caused changes in β-catenin signaling proteins similar to those observed in GPCs from AD patients. (jneurosci.org)
  • The links are also intriguing based on long-standing observations that β-catenin is associated in cells with presenilin proteins, a link whose functional consequence for synapses is still not clear. (alzforum.org)
  • As a signaling molecule, β-catenin supplies an activating domain to the T-cell factor/lymphoid enhancer-binding factor family of DNA-binding proteins and activates gene transcription. (aacrjournals.org)
  • We have identified the β-catenin-gal-3-binding sequences, which are in the NH 2 and COOH termini of the proteins encompassing amino acid residues 1 to 131 and 143 to 250, respectively. (aacrjournals.org)
  • α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4). (cellsignal.com)
  • The cytoplasmatic tail of the E-cadherin molecule binds to the proteins of the catenin family: p120-catenin, beta-catenin, and beta-catenin. (redorbit.com)
  • The catenins, α, β and γ, are proteins which bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. (scbt.com)
  • Addgene: Transformation by Wnt family proteins correlates with regulation of beta-catenin. (addgene.org)
  • In addition, we engineered intracellularly functional anti-β-catenin chromobodies by combining the binding moieties of the nanobodies with fluorescent proteins. (mcponline.org)
  • Additionally, other actin-associated proteins bind αE-catenin and could provide an indirect link to the actin cytoskeleton. (rupress.org)
  • However, in almost all cases, the molecular affinities and stoichiometries of these additional proteins in the cadherin-catenin complex have not been defined. (rupress.org)
  • β-Catenin (781 aa) binds E-cadherin and N-cadherin. (oxfordreference.com)
  • On the other hand, Wnt5a binds to the receptor complex of Fzd, Ror1/2 or Ryk and activates β-catenin-independent noncanonical signaling including Wnt/Ca 2+ and Wnt/planar cell polarity pathways 7 . (nature.com)
  • In adherens junctions, cadherin's cytoplasmic domain binds p120 catenin and beta-catenin, which in turn binds alpha-catenin. (nih.gov)
  • now report that, when in the cytoplasm, p120 catenin binds to Vav2, an exchange factor that activates Cdc42 and Rac1. (sciencemag.org)
  • Stablized β-catenin is translocated to the nucleus via Rac1 and other factors, where it binds to LEF/TCF transcription factors, displacing co-repressors and recruiting additional co-activators to Wnt target genes. (cellsignal.com)
  • The cytoplasmic domain of E-cadherin binds directly to either β-catenin or γ-catenin (also called plakoglobin). (uninet.edu)
  • Brannon M, Gomperts M, Sumoy L, Moon RT and Kimelman D (1997) A β‐catenin/Xtcf‐3 complex binds to the siamoispromoter to regulate dorsal axis specification in Xenopus. (els.net)
  • Gal-3 binds to β-catenin/Tcf complex, colocalizes with β-catenin in the nucleus, and induces the transcriptional activity of Tcf-4 as determined by the TOP/FOPFLASH reporter system. (aacrjournals.org)
  • PRI-724 binds specifically to CBP but not the related transcriptional coactivator p300, thereby disrupting the interaction of CBP with β-catenin. (selleckchem.com)
  • γ-catenin, also known as plakoglobin, binds with α-catenin and N-cadherin. (scbt.com)
  • αE-catenin binds the cell-cell adhesion complex of E-cadherin and β-catenin (β-cat) and regulates filamentous actin (F-actin) dynamics. (rupress.org)
  • Wnt regulates bone formation through β-catenin-dependent canonical and -independent noncanonical signaling pathways. (nature.com)
  • β-Catenin-mediated signalling regulates cancer cell metabolism and is associated with tumour-initiating cells in multiple malignancies. (springer.com)
  • Beiter K, Hiendlmeyer E, Brabletz T, Hlubek F, Haynl A, Knoll C, Kirchner T and Jung A 2005 beta-Catenin regulates the expression of tenascin-C in human colorectal tumors. (springer.com)
  • Our data indicate that β-catenin regulates myeloid cell motility and adhesion and that β-catenin-mediated macrophage motility contributes to the number of mesenchymal cells and ultimate scar size following cutaneous injury. (jci.org)
  • Conventional Wnt signaling causes β-catenin accumulation in a complex with the transcription factor TCF/LEF that regulates target gene expression ( Fig. 1 ). (sciencemag.org)
  • While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). (cellsignal.com)
  • In mammalian cells and in the Zebrafish, Bcl9-2 regulates binding of β -catenin to the adhesion complex or its presence in the nucleus, by interacting with the tyrosine phosphorylated form of β -catenin ( Brembeck, 2004 ). (stanford.edu)
  • Signaling by Wnt/beta-catenin regulates self-renewal of tissue stem cells in the gut and, when activated in the embryonic bronchiolar epithelium, leads to stem cell expansion. (biomedsearch.com)
  • In cells, to test whether αE-catenin regulates actin dynamics independently of the cadherin complex, the cytosolic αE-catenin pool was sequestered to mitochondria without affecting overall levels of αE-catenin or the cadherin-catenin complex. (rupress.org)
  • These results demonstrate that the cytosolic pool of αE-catenin regulates actin dynamics independently of cell-cell adhesion. (rupress.org)
  • δ-Catenin-1 (p120, p120ctn, cadherin-associated src substrate, 938 aa) is involved in cell-cell adhesion complexes together with E-*cadherin, α-, β-, and γ-catenins. (oxfordreference.com)
  • Inhibition of RhoA by p120 catenin. (nih.gov)
  • The catenin p120 has also been implicated in cadherin clustering through an unknown mechanism. (nih.gov)
  • Expression of non-membrane-targeted alpha-catenin produced only a slight effect, while both membrane-targeted and non-targeted p120 and beta-catenin were ineffective in this assay. (nih.gov)
  • Right panel represents the scheme of chimeric fusion constructs of α-catenin (A), β-catenin (B) or p120 catenin (C) with either enhanced GFP (EGFP) only, or the extracellular-transmembrane domain of the interleukin-2 receptor α-subunit and EGFP. (nih.gov)
  • Effect of expression of membrane-targeted and cytoplasmic p120 catenin on the microtubule density in the centrosome-lacking cytoplasts. (nih.gov)
  • The authors suggest that the distribution of p120 catenin between the cadherin-bound state and the cytoplasmic pool is important in determining cell motility. (sciencemag.org)
  • More recently, attention has been directed towards p120-catenin, which in conjunction with the p120-catenin sub-family members ARVCF- and delta-catenins, are the subjects of this review. (nih.gov)
  • Although the requirement for vertebrate versus invertebrate p120-catenin are at variance, vertebrate p120-catenin sub-family members may each inter-link cadherin, cytoskeletal and gene regulatory functions in embryogenesis and disease. (nih.gov)
  • Vascular endothelial-cadherin (VE-cadherin) and p120 constitute the cadherin-catenin complex (CCC) [ 1 , 5 ], which is dynamically regulated by many intracellular and extracellular signals [ 6 ]. (hindawi.com)
  • The association of p120 catenin (p120) with the juxtamembrane domain (JMD) of the cadherin cytoplasmic tail is critical for the surface stability of cadherin-catenin cell-cell adhesion complexes. (rcsb.org)
  • Catenins, namely β-catenin, plakoglobin and p120 CTN are good substrates for tyrosine kinases. (uninet.edu)
  • The cases were studied immunohistochemically, using specific monoclonal antibodies against E-cadherin, α-catenin, β-catenin, γ-catenin and p120 CTN . (uninet.edu)
  • The p120 catenin partner Kaiso is a DNA methylation-dependent transcriptional repressor. (sigmaaldrich.com)
  • As Kaiso associates with p120 catenin, Kaiso may link events at the cell surface with DNA methylation-dependent gene silencing. (sigmaaldrich.com)
  • F9 cells were genetically engineered to lack β-catenin, resulting in increased association of plakoglobin with E-cadherin. (wikipedia.org)
  • In F9 cells lacking both β-catenin and plakoglobin, very little E-cadherin and α-catenin accumulated at the cell surface. (wikipedia.org)
  • Mice lacking plakoglobin have cell adhesion defects in many tissues, although β-catenin substitutes for plakoglobin at many cellular junctions. (wikipedia.org)
  • Signaling and adhesion activities of mammalian beta-catenin and plakoglobin in Drosophila. (ebi.ac.uk)
  • γ-Catenin (plakoglobin) is a close homologue of β-catenin. (uninet.edu)
  • These gene products are important in determining cell fates during normal development and in maintaining homeostasis, or they can lead to de-regulated growth in disorders like cancer by responding to mutations in β-catenin, APC or Axin, each of which can lead to this de-regulated β-catenin level stabilization in cells. (wikipedia.org)
  • Mosimann C, Hausmann G, Basler K. Beta-catenin hits chromatin: regulation of Wnt target gene activation. (springer.com)
  • Activation of Wnt signaling inactivates GSK3, allowing β-catenin to accumulate in the cytoplasm and eventually to translocate to the nucleus so as to affect gene expression. (sciencemag.org)
  • Abe K and Takeichi M 2007 NMDA-receptor activation induces calpain-mediated β-Catenin cleavages for triggering gene expression. (springer.com)
  • 2001 The chromatin remodelling factor Brg-1 interacts with beta-catenin to promote target gene activation. (springer.com)
  • Here, cell lineage tracing revealed that cells in which β-catenin is transcriptionally active express a gene profile that is characteristic of the myeloid lineage. (jci.org)
  • Mice harboring a macrophage-specific deletion of the gene encoding β-catenin exhibited insufficient skin wound healing due to macrophage-specific defects in migration, adhesion to fibroblasts, and ability to produce TGF-β1. (jci.org)
  • We found that the treatment of HC GPCs with exogenous Aβ induced changes in neurogenesis, β-catenin signaling, and proneural gene expression that were similar to those exhibited by AD GPCs and their progeny. (jneurosci.org)
  • With GSK‐3 suppressed, β‐catenin accumulates, enters nuclei and forms complexes with Tcf and LEF family members (X) which then regulate gene expression (arrows in nucleus denotes gene activation). (els.net)
  • It is well established that mutations within the regulatory GSK-3β region lead to stabilized β-catenin and constitutive β-catenin/TCF-dependent gene activation. (oregonstate.edu)
  • The signaling potential of various β-catenin was analyzed in a gene reporter assay by co-transfection with a hTcf cDNA with a reporter plasmid containing a Tcf-dependent promoter (TOPFlash). (oregonstate.edu)
  • There was a significant enhancement of the reporter gene activity with all β-catenin mutants compared to WT β-catenin after 48 hours of transfection. (oregonstate.edu)
  • MAIN METHODS: Gene knockdown experiments using small interfering RNA (siRNA) transfection were conducted to elucidate the crucial role of β-catenin in the anti-adipogenic effects of shikonin. (biomedsearch.com)
  • Activating mutations in the human β -catenin gene have been found in human colon cancer and melanomas ( Morin et al, 1997 ) These mutations alter specific β -catenin residues important for GSK3 phosphorylation and stability. (stanford.edu)
  • Interestingly, one of them (HMP-2) is dedicated to adhesion only, whereas BAR-1 and WRM-1 act in Wnt signaling( Korswagen 2000 Natarajan 2001) Schneider et al (2003) postulate that a single, β -catenin gene fulfilled both adhesion and signaling functions in the last common ancestor of metazoans some 700 million years ago. (stanford.edu)
  • When Wnt ligands bind the Frizzled family of extracellular receptors, β-catenin is stabilized and translocates to the nucleus, where it promotes gene transcription by forming a complex with the TCF family of transcription factors. (fluidigm.com)
  • We have used transgenic and cell type-specific knockout strategies to determine roles for beta-catenin-regulated gene expression in normal maintenance and repair of the bronchiolar epithelium. (biomedsearch.com)
  • We constructed a Petri net model of Wnt/β-catenin signaling including main players from the plasma membrane (WNT ligands and receptors), cytoplasmic effectors and the downstream negative feedback target gene AXIN2. (uva.nl)
  • Most metazoans possess a single gene for β-catenin, which is also a transcriptional coactivator in Wnt signaling. (osti.gov)
  • These complexes, which help regulate cell growth in addition to creating and maintaining epithelial layers, are known as adherens junctions and they typically include at least cadherin, β-catenin, and α-catenin. (wikipedia.org)
  • α-catenin participates in the formation and stabilization of adherens junctions by binding to β-catenin-cadherin complexes in the cell. (wikipedia.org)
  • Meanwhile, β-catenin was shown to be an integral component of cadherin-mediated cell adhesion complexes. (springer.com)
  • The binding of Wnt to receptor complexes activates β-catenin-dependent canonical and β-catenin-independent noncanonical signaling pathways 5 . (nature.com)
  • These cadherin derivatives formed complexes with β-catenin and protected it from degradation. (pnas.org)
  • Figure: This image depicts the ornamental braiding (Pan Kou) for fastening the front of traditional Chinese clothing in watercolor, as an analogy of how cadherin/catenin complexes regulate the synapse maturation. (eurekalert.org)
  • A recent study conducted by Dr. YU Xiang's lab at the Institute of Neuroscience of the Chinese Academy of Sciences uncovered an asymmetric role for symmetric cadherin/catenin cell adhesion complexes in functional synapse formation in the neocortex. (eurekalert.org)
  • The effect of the cadherin/catenin complexes requires p140Cap, a novel β-catenin interacting partner. (eurekalert.org)
  • They showed the presence of cadherin/catenin/p140Cap complexes. (eurekalert.org)
  • The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. (thermofisher.com)
  • The mechanisms responsible for specification of the dorsal axis include a post‐fertilization cortical rotation that promotes a dorsal accumulation of β‐catenin, and the activation of a cascade of regulatory genes by the complexes of β‐catenin and HMG box transcription factors that start the process of gastrulation. (els.net)
  • Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. (scbt.com)
  • It has been shown that the transmembrane phosphatase PTPµ associates with catenin/cadherin complexes and may regulate complex signaling. (scbt.com)
  • The different tasks of β-catenin are orchestrated by its subcellular localization and participation in multiprotein complexes. (mcponline.org)
  • We selected nanobodies recognizing the N-terminal, core or C-terminal domain of β-catenin and applied these new high-affinity binders as capture molecules in sandwich immunoassays and co-immunoprecipitations of endogenous β-catenin complexes. (mcponline.org)
  • Catenin-cadherin complexes also are associated with sites of contact between blastomeres in vertebrate and invertebrate embryos. (rupress.org)
  • 2 Following nuclear translocation, β-catenin complexes with the T-cell factor (TCF)/lymphoid enhancer factor (LEF) transcriptional regulators and promotes activation of proto-oncogenic Wnt target genes, like c-myc, cyclinD1 and survivin ( http://web.stanford.edu/group/nusselab/cgi-bin/wnt/target_genes ). (haematologica.org)
  • The intrinsic asymmetry is further emphasized by depicting the pre-synaptic cadherin/catenin complexes (top left) more ornate than the post-synaptic ones (bottom right). (medicalxpress.com)
  • Following phosphorylation of the N-terminal Ser and Thr residues of β-catenin, BTRC promotes its ubiquitination, which causes it to be degraded by the TrCP/SKP complex. (wikipedia.org)
  • Upon phosphorylation by GSK3β, β-catenin associates with a ubiquitin ligase (E3) complex, resulting in its ubiquitination and proteolysis. (sciencemag.org)
  • Two groups report that a phosphorylation-independent mechanism can lead to the destruction of β-catenin. (sciencemag.org)
  • Phosphorylation of β-catenin by the APC-axin-glycogen synthase kinase 3β complex ( 22 , 23 ) leads to its degradation by the ubiquitin-proteasome system ( 6 , 24 ). (pnas.org)
  • Moreover, overexpressing an N-cadherin mutant lacking the extracellular domain or β-catenin mutants with an altered phosphorylation site alters spine morphology and synaptic efficacy ( 23 - 26 ). (pnas.org)
  • Importantly, researchers have found β-catenin point mutations in human tumors that prevent GSK-3β phosphorylation and thus lead to its aberrant accumulation. (cellsignal.com)
  • The complex specifies a ß -TrCP recognition site on ß -catenin by phosphorylation of a conserved Ser / Thr-rich sequence near the amino terminus. (stanford.edu)
  • Phosphorylation requires scaffolding of GSK-3 and CK1 and ß - catenin by Axin. (stanford.edu)
  • Aftter phosphorylation and u biquitination, ß -catenin is degraded by the proteasome. (stanford.edu)
  • In addition, fear training enhanced inhibitory phosphorylation of the kinase GSK3β, thus preventing the kinase from destabilizing β-catenin. (alzforum.org)
  • In non-stimulated cells, β-catenin is tightly down-regulated by GSK-3β-dependent phosphorylation at Ser and Thr residues, followed by rapid ubiquitination and proteasomal degradation. (oregonstate.edu)
  • Thus, a major hypothesis of this thesis was that substitution mutations at codon 32 of β-catenin interfere with phosphorylation and ubiquitination of β-catenin. (oregonstate.edu)
  • Thus, although codon 32 of β-catenin is not a direct target for phosphorylation, results from this thesis suggested that it affects the phosphorylation and ubiquitination of the adjacent Ser-33 residue of β-catenin, which is a direct target of GSK-3β. (oregonstate.edu)
  • In addition, these results showed for the first time that the phosphorylation step of β-catenin is not enough to regulate transcriptional activity, and that β-catenin still needs to be ubiquitinated for successful down-regulation. (oregonstate.edu)
  • IWP-2 blocks Wnt-dependent phosphorylation of Lrp6 receptor and Dvl2, and β-catenin accumulation. (selleckchem.com)
  • Many natural products have been characterized as inhibitors of the β-catenin signaling through down-regulating β-catenin expression, modulating its phosphorylation, promoting its ubiquitination and proteasomal degradation, inhibiting its nuclear translocation, or other molecular mechanisms. (frontiersin.org)
  • Phosphorylation of a Ser/Thr-rich region in the cadherin tail dramatically enhances affinity for β-catenin and promotes cell-cell adhesion in cell culture systems, but its importance has not been demonstrated in vivo. (osti.gov)
  • β-catenin acts by anchoring the actin cytoskeleton to the junctions, and may possibly aid in contact inhibition signaling within the cell. (wikipedia.org)
  • In adherens-type junctions, β-catenin interacts with the cytoplasmic domains of cadherin molecules and links them, through α-catenin, to the actin cytoskeleton ( 1 , 2 ). (pnas.org)
  • The normal glutamatergic synaptic response is maintained by postsynaptic β-catenin in a cadherin-dependent manner and requires the C-terminal PDZ-binding motif of β-catenin but not the link to the actin cytoskeleton. (pnas.org)
  • Analysis of αN-catenin knockout mice has also revealed a requirement for the cadherin link to the actin cytoskeleton in regulating spine dynamics ( 27 ). (pnas.org)
  • Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. (thermofisher.com)
  • This complex is coupled to the cytoskeleton by α-catenin via its association with actin and β-catenin or γ-catenin. (uninet.edu)
  • β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). (cellsignal.com)
  • and therefore, it was assumed that αE-catenin statically links the cadherin-catenin complex to the actin cytoskeleton ( Gates and Peifer, 2005 ). (rupress.org)
  • While less attention is directed at α-catenin in studies involving cell adhesion, it is nonetheless an important player in cellular organization, function and growth. (wikipedia.org)
  • F9 embryonal carcinoma cells are similar to the P19 cells shown in Figure 1 and normally have cell-to-cell adhesion mediated by E-cadherin with β-catenin bound to the cytoplasmic domain of E-cadherin. (wikipedia.org)
  • Mice engineered to specifically have vascular endothelium cells deficient in β-catenin showed disrupted adhesion between vascular endothelial cells. (wikipedia.org)
  • Keratinocytes engineered to not express alpha-catenin have disrupted cell adhesion and activated NF-κB. (wikipedia.org)
  • Three different forms of catenin (designated alpha, beta and gamma) comprise the cytoplasmic domain of the cadherin cell-cell adhesion complex [ PMID: 7945318 ]. (ebi.ac.uk)
  • Beta-catenin forms a cadherin/beta-catenin/alphaE-catenin complex that can tether the tripartite adhesion complex and regulate actin dynamics [ PMID: 23739176 ]. (ebi.ac.uk)
  • The precise contribution of the cadherin-β-catenin synapse adhesion complex in the functional and structural changes associated with the pre- and postsynaptic terminals remains unclear. (pnas.org)
  • Balsamo J, Leung T, Ernst H, Zanin MK, Hoffman S and Lilien J 1996 Regulated binding of PTP1B-like phosphatase to N-cadherin: control of cadherin-mediated adhesion by dephosphorylation of beta-catenin. (springer.com)
  • The most deeply studied member of the larger catenin family is beta-catenin, whose contributions span areas including cell adhesion and intracellular signaling/ transcriptional control. (nih.gov)
  • for maintenance of normal intercellular adhesion, an intact E-cadherin/catenin complex is required. (uninet.edu)
  • The E-cadherin/catenin complex is implicated not only in cell-cell adhesion, but in directed migration during epithelial wound healing, presumably through contact inhibition of membrane ruffling. (uninet.edu)
  • α-Catenin functions through maintenance of cell-cell adhesion and may interference with β-catenin/Tcf/DNA complex formation and β-catenin signalling in the nucleus. (uninet.edu)
  • The expression of such cell-cell adhesion molecules as E-cadherin and E-cadherin complex-associated catenins has not been investigated to date. (uninet.edu)
  • In the lead story, Kimberly Maguschak and Kerry Ressler of Emory University in Atlanta, Georgia, implicate the adhesion receptor and signaling molecule β-catenin in the synaptic restructuring that underlies long-term memory in vivo. (alzforum.org)
  • β-Catenin plays essential roles in both intercellular adhesion and signal transduction. (aacrjournals.org)
  • Here, we assemble evidence of possible interrelations between Wnt and other growth factor signaling, β-catenin functions, and cadherin-mediated adhesion. (sciencemag.org)
  • This review explores intriguing connections between Wnt and other growth factor signals, β-catenin distribution, and cadherin-mediated cell adhesion ( Fig. 1 , inset). (sciencemag.org)
  • The insert displays possible levels of interactions between Wnt signaling and cadherin-mediated adhesion (dotted lines) and the central role of β-catenin in both processes that are the focus of the review. (sciencemag.org)
  • Deletions in the cytoplasmic domain of E-Cadherin which eliminate catenin binding also result in a loss of cell adhesion, indicating that this binding is essential for E-Cadherin function. (fishersci.com)
  • Thus the APC-Catenin complex may be affecting the transmission of contact inhibition signals and/or the regulation of cell adhesion. (fishersci.com)
  • ABSTRACT In this article, we show, using a mathematical multiscale model, how cell adhesion may be regulated by interactions between E-cadherin and beta-catenin and how the control of cell adhesion may be related to cell migration, to the epithelial- mesenchymal transition and to invasion in populations of eukaryotic cells. (redorbit.com)
  • β-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. (scbt.com)
  • This putative catenin-cadherin system is not essential for general cell adhesion in the C. elegans embryo, but rather mediates specific aspects of morphogenetic cell shape change and cytoskeletal organization. (rupress.org)
  • Sequestering cytosolic αE-catenin to mitochondria alters lamellipodia architecture and increases membrane dynamics and cell migration without affecting cell-cell adhesion. (rupress.org)
  • Studies of αE-catenin in vivo have also suggested functions independent of cadherin-mediated cell-cell adhesion. (rupress.org)
  • It has been suggested that alpha-catenin does not bind with high affinity to both actin filaments and the E-cadherin-beta-catenin complex at the same time. (wikipedia.org)
  • The amino acid sequence of alpha-catenin has sequence similarity to that of vinculin. (wikipedia.org)
  • Three alpha-catenin genes are expressed in humans: CTNNA1, alpha-1-catenin (also called alpha-E-catenin) CTNNA2, alpha-2-catenin (also called alpha-N-catenin) CTNNA3, alpha-3-catenin (also called alpha-T-catenin) Catenin alpha+Catenin at the US National Library of Medicine Medical Subject Headings (MeSH) Cooper, Geoffrey M. (2000). (wikipedia.org)
  • Signaling function of alpha-catenin in microtubule regulation. (nih.gov)
  • Only membrane targeting of alpha-catenin led to a significant increase in microtubule length and density in centrosome-free cytoplasts. (nih.gov)
  • Together, these findings suggest that alpha-catenin is able to regulate microtubule dynamics in a centrosome-independent manner. (nih.gov)
  • Peripheral mechanisms take central stage in microtubule dynamics: commentary on "Signaling function of alpha-catenin in microtubule regulation" by Shtutman et al. (nih.gov)
  • Your search returned 780 alpha Catenin Antibodies across 44 suppliers. (biocompare.com)
  • This complex inhibits the kinase activity of GSK-3β, which in turn induces the accumulation of β-catenin in the target cells. (nature.com)
  • The accumulation of β-catenin leads to its translocation into the nucleus, where it interacts with T-cell factor/lymphoid enhancer factor (Tcf/Lef) family members to initiate the transcription of target genes. (nature.com)
  • The failure of this degradation in cells expressing mutant APC or β-catenin leads to the accumulation of β-catenin and is common in human colon cancer and melanoma ( 25 - 27 ). (pnas.org)
  • 5 show surprising findings that suggest a previously unappreciated role for nuclear FOXO3a in promoting metastasis in the context of nuclear β-catenin accumulation, a common occurrence in colon cancers. (nature.com)
  • Aberrant WNT/β-catenin signaling by lost expression and DNA methylation of APC, and accumulation of active nonphosphorylated β-catenin was observed in the analyzed PCs. (sigmaaldrich.com)
  • Dysregulation of UBA1 and subsequent ubiquitination pathways led to β-catenin accumulation, and pharmacological inhibition of β-catenin robustly ameliorated neuromuscular pathology in zebrafish, Drosophila , and mouse models of SMA. (jci.org)
  • Dorsal accumulation of β‐catenin in Xenopus embryos. (els.net)
  • In addition, inhibition of the proteasome activity by ALLN was associated with accumulation of cytosolic β-catenin, which was transcriptionally inactive. (oregonstate.edu)
  • As a consequence, GSK does not phosphorylate β -catenin anymore, releasing it from the Axin complex and accumulation ( Salic, 2000) .The stabilized β -catenin then enters the nucleus to interact with TCF. (stanford.edu)
  • Moreover, the chromobody signal allowed us to trace the accumulation of diffusible, hypo-phosphorylated β-catenin in response to compound treatment in real time using High Content Imaging. (mcponline.org)
  • This leads to the accumulation of hypo-phosphorylated β-catenin in the cytoplasm followed by its translocation into the nucleus where it interacts with members of the Lymphoid enhancer factor/T-cell factor (LEF/TCF) 1 family to activate transcription of Wnt-responsive genes ( 13 ⇓ ⇓ ⇓ - 17 ). (mcponline.org)
  • Upon Wnt ligand binding to the Wnt receptors (Frizzled and LRP5/6), the DC becomes saturated with phosphorylated β-catenin (which cannot be degraded) resulting in cytosolic accumulation of non-phosphorylated β-catenin. (haematologica.org)
  • The findings have important implications-a new cooperative interaction of these two fundamental pathways in the nucleus in mediating metastasis, along with clinical relevance for patients with colorectal cancer and dual elevation of FOXO3a and β-catenin, as they may respond poorly to PI3K-AKT inhibitors. (nature.com)
  • β-catenin is more often associated with development and cell survival pathways, but by both pharmacologic and genetic approaches in mice, the investigators find that its stabilization is essential for the conversion of new information into long-term memories in the amygdala. (alzforum.org)
  • Wnt/β-catenin and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling both are conserved pathways that regulate a variety of biological processes throughout the development and adult lifetime of mammals. (frontiersin.org)
  • The Wnt/β-catenin and NF-κB signaling pathways regulate, through independent cascades, the expression of different subsets of target genes controlling cell proliferation, cell survival, and differentiation. (frontiersin.org)
  • Finally, inhibitor of PI3K/Akt signaling pathways inactivated the EGF-β-catenin-induced EMT. (dovepress.com)
  • We then discuss the opportunities for using aptamers in other complex pathways, including Wnt/β-catenin, and focus on Transforming Growth Factor-β/Smad family signaling. (mdpi.com)
  • Grigoryan T, Wend P, Klaus A, Birchmeier W. Deciphering the function of canonical Wnt signals in development and disease: conditional loss- and gain-of-function mutations of beta-catenin in mice. (springer.com)
  • Four of the five mutations involved serine or threonine residues implicated in the degradation of β-catenin. (aacrjournals.org)
  • Mutational analysis of multiple regions from several tumor samples showed that the β-catenin mutations were present focally and therefore may occur during tumor progression. (aacrjournals.org)
  • Furthermore, it has been shown that amino acids adjacent to codon 33, namely 32 and 34 of β-catenin, are hotspots for substitution mutations in carcinogen-induced animal tumors. (oregonstate.edu)
  • Tooth agenesis is one of predominant developmental anomalies in humans, usually affecting the permanent dentition generated by sequential tooth formation, and in most cases caused by mutations perturbing epithelial Wnt/β-catenin signaling. (biologists.org)
  • Our data indicate that increased mesenchymal Wnt signaling inhibits the sequential formation of teeth, and suggest that Axin2 / Runx2 antagonistic interactions modulate the level of mesenchymal Wnt/β-catenin signaling, underlying the contrasting dental phenotypes caused by human AXIN2 and RUNX2 mutations. (biologists.org)
  • Heteroduplex was used to investigate mutations in beta-catenin. (mdpi.com)
  • Eventually, some of this accumulated β-catenin will move into the nucleus with the help of Rac1. (wikipedia.org)
  • Expression of N-cadherin and an interleukin 2 receptor/cadherin chimera in SW480 cells relocated β-catenin from the nucleus to the plasma membrane and reduced transactivation. (pnas.org)
  • The cytoplasmic tails of N- or E-cadherin colocalized with β-catenin in the nucleus, and suppressed the constitutive LEF-1-mediated transactivation, by blocking β-catenin-LEF-1 interaction. (pnas.org)
  • These results indicate that β-catenin binding to the cadherin cytoplasmic tail either in the membrane, or in the nucleus, can inhibit β-catenin degradation and efficiently block its transactivation capacity. (pnas.org)
  • In addition, β-catenin can translocate into the nucleus ( 3 - 7 ), where it is involved, together with transcription factors of the lymphocyte enhancer-binding factor 1 (LEF)/T cell factor (TCF) family, in the expression of specific genes ( 8 , 9 ). (pnas.org)
  • In the nucleus, β-catenin functions as both activator and repressor of transcription in a context-dependent manner. (springer.com)
  • Figure 1: Concomitant localization of FOXO3a and β-catenin in the nucleus mediate progression and metastasis in colorectal cancer upon PI3K or AKT inhibition. (nature.com)
  • In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. (thermofisher.com)
  • It can be found at the membrane and in the nucleus like β-catenin. (uninet.edu)
  • Consequently, the unphosphorylated form of β-catenin accumulates in the cytoplasm, making it available for entry into the nucleus. (biologists.org)
  • Once in the nucleus, β-catenin interacts with Tcf/Lef transcriptional co-activators to promote the expression of target genes ( Nelson and Nusse, 2004 ). (biologists.org)
  • However, the molecular mechanism that targets β-catenin to the nucleus is still unclear. (aacrjournals.org)
  • Similarly to β-catenin, endogenous gal-3 is found in the cytoplasm, on the cell surface, and in the nucleus, where it undergoes post-translational modification by the addition of a phosphate group catalyzed by CK1. (aacrjournals.org)
  • The main effector of the Wnt signaling, beta-catenin, was up-regulated in 56%, and transferred to the nucleus in 36% of metastases. (mdpi.com)
  • Pre-synaptic β-catenin is predominant during functional synapse formation and mediates dendritic spine stabilization through N-cadherin-dependent anterograde trans-synaptic signaling. (eurekalert.org)
  • Using live imaging, the researchers showed that β-catenin is required for spine stabilization and not de novo formation. (eurekalert.org)
  • Here, we show that ectopic stabilization of β-catenin within mouse pancreatic epithelium can have divergent effects on both organ formation and growth. (biologists.org)
  • Robust stabilization of β-catenin during early organogenesis drives changes in hedgehog and Fgf10 signaling and induces a loss of Pdx1 expression in early pancreatic progenitor cells. (biologists.org)
  • This allowed us to determine how β-catenin stabilization in these distinct temporal/spatial domains of the pancreatic epithelium affected organogenesis and adult organ function. (biologists.org)
  • The destruction complex is inhibited during active WNT signaling, leading to β-catenin stabilization and induction of β-catenin/TCF target genes. (uva.nl)
  • In this study we investigated the mechanism and effect of β-catenin stabilization during active and hyperactive WNT signaling in a combined in silico and in vitro approach. (uva.nl)
  • We used this experimentally validated model to get further insights into the effect of the negative feedback regulator AXIN2 upon WNT stimulation and observed an attenuated β-catenin stabilization. (uva.nl)
  • In vitro, FGF-7 induces stabilization and nuclear translocation of ß-catenin in human corneal epithelial cells. (novapublishers.com)
  • At this point, β-catenin becomes a coactivator for TCF and LEF to activate Wnt genes by displacing Groucho and HDAC transcription repressors. (wikipedia.org)
  • In these resistant cancer cells, nuclear FOXO3a and β-catenin act together to regulate a defined set of target genes mediating cell scattering and metastasis. (nature.com)
  • The authors conducted an immunohistochemical analysis of the expression of β-catenin, upstream modulators (HER-2/ neu , Met, and epidermal growth factor receptor [EGFR]), downstream target genes (cyclin D1 and matrix metalloproteinase-7 [MMP7]), and p53 on a tissue microarray of 346 lymph node-negative breast carcinomas. (wiley.com)
  • β -catenin can convert TCF into a transcriptional activator of the same genes that are repressed by TCF alone (reviewed in Nusse, 1999 ). (stanford.edu)
  • There are three β -catenin genes in C. elegans. (stanford.edu)
  • Catenins interact closely with E-cadherin molecules in cells, and are therefore logical candidate genes for mutation screening in HDGC families. (aacrjournals.org)
  • Phosphorylated β-catenin subsequently undergoes ubiquitination and degradation. (nature.com)
  • report that Siah-1 also interacts with APC, which is required for Siah-1-dependent degradation of β-catenin. (sciencemag.org)
  • We show that expression of the cytoplasmic tail of cadherin, either membrane bound or soluble, protects endogenous β-catenin from degradation and blocks its transactivation capability. (pnas.org)
  • When cells detach from one another, beta-catenin is released into the cytoplasm, targeted for degradation, and downregulated. (redorbit.com)
  • In the absence of Wnt signaling, the kinases CK1 and GSK3β phosphorylate β-catenin at multiple sites, leading to proteasomal degradation. (fluidigm.com)
  • moreover, inhibition of AKT signaling promoted scattering and metastasis of xenografted colon tumor cells with elevated β-catenin. (nature.com)
  • therefore, pharmacological inhibition of β-catenin in these animals failed to prevent systemic pathology in peripheral tissues and organs, indicating fundamental molecular differences between neuromuscular and systemic SMA pathology. (jci.org)
  • Amelioration of neuromuscular pathology in zebrafish and Drosophila models of SMA following pharmacological inhibition of β-catenin signaling. (jci.org)
  • In U87 cell line, inhibition of β-catenin by siRNA suppressed EGF-induced proliferation, migration, invasiveness, and the expression of EMT activators (Snail and Slug). (dovepress.com)
  • This suppression of β-catenin transcriptional capacity was independent of ALLN-associated apoptosis in the transfected cells. (oregonstate.edu)
  • α-catenin also plays a role in regulating β-catenin-dependent transcriptional activity, affecting differentiation and response to Wnt signaling. (cellsignal.com)
  • KEY FINDINGS: Shikonin prevented the down-regulation of β-catenin and increased the level of its transcriptional product, cyclin D1, during adipogenesis of 3T3-L1 cells, preadipocytes originally derived from mouse embryo. (biomedsearch.com)
  • Comparison of interacting factors between Wnt-responsive cells (high nuclear β-catenin) versus Wnt-unresponsive cells (low nuclear β-catenin) suggested the transcriptional partner, LEF-1, could direct the nuclear-localization of β-catenin. (haematologica.org)
  • Furthermore, LEF-1 knockdown perturbed β-catenin nuclear-localization and transcriptional activation in Wnt-responsive cells. (haematologica.org)
  • Conversely, LEF-1 overexpression was able to promote both nuclear-localization and β-catenin-dependent transcriptional responses in previously Wnt-unresponsive cells. (haematologica.org)
  • β‐catenin (orange) accumulates in the cytoplasm on the prospective dorsal side of the embryo (right) opposite the sperm entry point (left). (els.net)
  • β-Catenin expression was observed in the membrane and/or cytoplasm without any significant nuclear expression. (wiley.com)
  • Ab 15153 stains gamma catenin on the cell membrane and in the cytoplasm of breast carcinoma. (genetex.com)
  • For the first time, we were able to visualize the subcellular localization and nuclear translocation of endogenous β-catenin in living cells using these chromobodies. (mcponline.org)
  • 5 showed that concurrent expression of ectopic activated FOXO3a together with β-catenin promoted cell scattering in vitro and metastasis in vivo . (nature.com)
  • Published in the 28 September Nature Neuroscience, the new data are consistent with previous in-vitro studies showing a role for β-catenin in synapse remodeling. (alzforum.org)
  • Importantly, Wnt/β-catenin activity is transiently required in the AGM to generate long-term HSCs and to produce hematopoietic cells in vitro from AGM endothelial precursors. (rupress.org)
  • In the present review, we comprehensively discuss the natural product β-catenin inhibitors, their in vitro and in vivo anticancer activities, and underlying molecular mechanisms. (frontiersin.org)
  • In vitro, lack of TREM2 impairs proliferation and β-catenin activation in osteoclast precursors (OcP) in response to M-CSF. (jimmunol.org)
  • Corroborating the importance of a balanced proliferation and differentiation of OcP for bone homeostasis, we show that conditional deletion of β-catenin in OcP also results in reduced OcP proliferation and accelerated osteoclastogenesis in vitro as well as osteopenia in vivo. (jimmunol.org)
  • In vitro, binding of αE-catenin to the E-cadherin-β-cat complex lowers αE-catenin affinity for F-actin, and αE-catenin alone can bind F-actin and inhibit Arp2/3 complex-mediated actin polymerization. (rupress.org)
  • 2018) Beta-Catenin. (springer.com)
  • These β-catenin signaling inhibitors have shown anticancer efficacy in preclinical settings, and some of them have entered clinical trials, such as PRI-724 ( Krishnamurthy and Kurzrock, 2018 ). (frontiersin.org)
  • The report then estimates 2018-2023 market development trends of Beta Catenin industry. (reportsnreports.com)
  • β-catenin is an adherens junction and signaling molecule with diverse roles in the regulation of cell structure and fate. (pnas.org)
  • β-catenin-mediated signaling also can be affected by the association of β-catenin with the tumor suppressor molecule adenomatous polyposis coli (APC) ( 17 , 18 ) and their further interaction with axin ( 19 , 20 ) and glycogen synthase kinase 3β ( 21 ). (pnas.org)
  • These observations suggest that the cadherin-catenin complex could potentially regulate aspects of synapse function beyond contributing to synapse assembly in developing neurons. (pnas.org)
  • Additionally, β-catenin cooperates with several other transcription factors to regulate specific targets. (cellsignal.com)
  • In the absence of Wnt, a complex of APC, axin and glycogen synthase kinase-3β (GSK-3β) phosphorylates β-catenin. (nature.com)
  • Here we report a requirement for endogenous β-catenin in regulating synaptic strength and dendritic spine morphology in cultured hippocampal pyramidal neurons. (pnas.org)
  • Canonical Wnt/β-catenin signaling is frequently dysregulated in myeloid leukemias and is implicated in leukemogenesis. (haematologica.org)
  • Molenaar M, van de Wetering M and Oosterwegel M (1996) Xtcf‐3 transcription factor mediates β‐catenin induced axis formation in Xenopus embryos. (els.net)
  • Mice lacking β-catenin have defective embryos. (wikipedia.org)
  • Osteoblast-lineage cells from Wnt5a -deficient mice exhibited reduced Wnt/β-catenin signaling, which impaired osteoblast differentiation and enhanced adipocyte differentiation. (nature.com)
  • Takuya Murata of RIKEN BRC, the first author of the paper, says, "Because the amino acid sequence of beta-catenin is 100% identical in humans and mice, the nucleotide change we saw could cause the same mutation in humans. (medicalnewstoday.com)
  • Using Nex-Cre mice to conditionally knockout or overexpress β-catenin in all neocortical excitatory neurons, and recording from layer 2/3 (L2/3) pyramidal neurons of the mouse barrel cortex, researchers found that β-catenin bi-directionally regulated the frequency of miniature excitatory postsynaptic currents. (eurekalert.org)
  • In irradiated mice, only macrophages expressing β-catenin were able to rescue wound-healing deficiency. (jci.org)
  • To ask whether changes in β-catenin affect learning, the investigators treated mice with lithium, an inhibitor of GSK3β and thus a stabilizer of β-catenin, or made conditional knockouts to delete β-catenin in the amygdala. (alzforum.org)
  • Mice lacking β-catenin learned normally during conditioning, but did not remember the association in later trials. (alzforum.org)
  • We assessed the role of Wnt/β-catenin in both steps in gain- and loss-of-function models in mice. (jneurosci.org)
  • In this report, we show that mice bearing B cell-specific deletion of β-catenin have normal B cell development in bone marrow and periphery. (jimmunol.org)
  • While the diversity of catenin localizations and roles has been intriguing, it has also posed a challenge to the clear interpretation of loss- or gain-of-function developmental phenotypes. (nih.gov)
  • Although the α- and β-Catenins have been cloned, very little is known about their biochemical roles. (fishersci.com)
  • A recent study has shown that deletion of β-catenin within the pancreatic epithelium results in a loss of pancreas mass. (biologists.org)
  • Two independent laboratories recently reported divergent phenotypes resulting from the conditional deletion of β-catenin within the pancreatic epithelium. (biologists.org)
  • Necessity of beta-catenin signaling was tested through Cre-mediated deletion of Catnb exons 2-6 in airway epithelial cells. (biomedsearch.com)
  • We studied the effect of N-cadherin, and its free or membrane-anchored cytoplasmic domain, on the level and localization of β-catenin and on its ability to induce lymphocyte enhancer-binding factor 1 (LEF-1)-responsive transactivation. (pnas.org)
  • Cellular β-catenin exists in three different pools: membranous, cytoplasmic and nuclear. (springer.com)
  • Thus, β-catenin appears to possess a multifaceted nuclear function that may significantly impact tumour initiation and progression. (springer.com)
  • Applying AKT inhibitors to tumor cells with concomitant high nuclear expression of FOXO3a and β-catenin resulted in resistance to apoptosis and promotion of the metastatic program ( Fig. 1 ). (nature.com)
  • By analyzing tumor samples, the authors found that nuclear FOXO3a and β-catenin were frequently concurrently expressed in late-stage cancers and that this was associated with poor prognostic outcome. (nature.com)
  • Lastly, patient-derived sphere cultures and tumor xenografts showed differential sensitivity to an AKT inhibitor that was inversely correlated to their intrinsic nuclear β-catenin levels 5 . (nature.com)
  • We show here that β-catenin is nuclear and active in few endothelial nonhematopoietic cells closely associated with the emerging hematopoietic clusters of the embryonic aorta during mouse development. (rupress.org)
  • Pannus surgically removed from OSSN patients all had nuclear ß-catenin and exhibited excess FGF-7. (novapublishers.com)
  • When DVL1 and DVL3 were up-regulated the number of cases with nuclear beta-catenin significantly increased ( p = 0.0001). (mdpi.com)
  • however, some patients exhibit little or no nuclear β-catenin even where cytosolic β-catenin is abundant. (haematologica.org)
  • To investigate the factors mediating the nuclear-localization of β-catenin, we carried out the first nuclear/cytoplasmic proteomic analysis of the β-catenin interactome in myeloid leukemia cells and identified putative novel β-catenin interactors. (haematologica.org)
  • The relative levels of nuclear LEF-1 and β-catenin were tightly correlated in both cell lines and in primary AML blasts. (haematologica.org)
  • This is the first β-catenin interactome study in hematopoietic cells and reveals LEF-1 as a mediator of nuclear β- catenin level in human myeloid leukemia. (haematologica.org)
  • Maguschak and Ressler looked at the role of β-catenin in memory in vivo, using a fear conditioning paradigm. (alzforum.org)
  • There is a pretty large literature showing that β-catenin plays some role in synaptogenesis, but our data is the first to show it in vivo, in the animal," Ressler told ARF. (alzforum.org)
  • Collectively, these findings suggest that the cadherin-β-catenin complex is an integral component of synaptic strength regulation and plays a basic role in coupling synapse function and spine morphology. (pnas.org)
  • The results point to a dynamic regulation of β-catenin function in the process of synaptic remodeling and memory formation. (alzforum.org)
  • Taken together, these data suggest a previously unappreciated temporal/spatial role forβ -catenin signaling in the regulation of pancreas organ growth. (biologists.org)
  • In this review, we summarize the current evidence for both positive and negative regulation of NF-κB-mediated inflammation by Wnt/β-catenin signaling and elaborate on the underlying molecular mechanisms. (frontiersin.org)
  • The catenins may be involved in the regulation of cadherin function during tissue morphogenesis and tumorigenesis. (biologists.org)
  • Bortezomib induces osteoblast differentiation via Wnt-independent activation of β -catenin/TCF signaling," Blood , vol. 113, no. 18, pp. 4319-4330, 2009. (hindawi.com)
  • Knock-out of β-catenin inhibited hair-cell, as well as pillar-cell, differentiation from sensory progenitors but was not required to maintain a hair-cell fate once specified. (jneurosci.org)
  • Constitutive activation of β-catenin expanded sensory progenitors by inducing additional cell division and resulted in the differentiation of extra hair cells. (jneurosci.org)
  • Our data demonstrate that β-catenin plays a role in cell division and differentiation in the cochlear sensory epithelium. (jneurosci.org)
  • In this study, we showed that Pygo2 exhibited a declined expression pattern during adipocyte differentiation, resulting in an attenuated Wnt/β-Catenin output level. (diabetesjournals.org)
  • Functional knockout of beta-catenin had no impact on expression of Clara cell differentiation markers, mitotic index, or sensitivity of these cells to the Clara cell-specific toxicant, naphthalene. (biomedsearch.com)
  • We offer Catenin alpha Antibodies for use in common research applications: Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot. (novusbio.com)
  • Our Catenin alpha Antibodies can be used in a variety of model species: Human, Mouse, Xenopus. (novusbio.com)
  • Choose from our Catenin alpha polyclonal antibodies. (novusbio.com)
  • β-catenin and ERα could not be co-immunoprecipitated by ERα antibodies from lysates of E2-treated or untreated cells suggesting lack of direct physical interaction. (mdpi.com)
  • Of interest, apart from the increase in β-catenin levels in certain tumors, a reduction in E-cadherin levels also was found in many carcinomas ( 28 - 30 ), and the invasiveness of these tumor cells could be suppressed by overexpression of E-cadherin ( 31 , 32 ). (pnas.org)
  • β-catenin overexpression promoted spinogenesis in L2/3 pyramidal neurons. (eurekalert.org)
  • we found that the overexpression of β-catenin partially restored neurogenesis from AD GPCs, while the reduction of β-catenin using β-catenin siRNA decreased neurogenesis from HC GPCs. (jneurosci.org)
  • We then compared the phenotypes and expression patterns following β-catenin knockdown and Wnt5a overexpression during hair follicle regeneration induced by hair depilation and observed similar patterns. (medsci.org)
  • Hence, the intracellular actions of the cadherin-catenin complex could be regulated independently at the pre- and postsynaptic sides of the synaptic junction. (pnas.org)
  • The expression and intracellular localization of beta-catenin is altered in many types of cancers. (agilent.com)
  • Moreover, recent evidence indicates that the receptor for M-CSF, although not physically associated with DAP12, uses this adaptor to transmit certain intracellular signals, particularly to activate Syk and β-catenin ( 14 , 15 ). (jimmunol.org)
  • To fulfill these different tasks properly, well-balanced intracellular levels of β-catenin are required. (mcponline.org)
  • Thus, beta-catenin was not necessary for maintenance or efficient repair of the bronchiolar epithelium. (biomedsearch.com)
  • Exon 3 of beta-catenin was not targeted. (mdpi.com)
  • β-catenin is particularly interesting as it plays a dual role in the cell. (wikipedia.org)
  • For instance, when an epithelial layer is complete and the adherens junctions indicate that the cell is surrounded, β-catenin may play a role in telling the cell to stop proliferating, as there is no room for more cells in the area. (wikipedia.org)
  • α-catenin, vinculin, and F-actin in strengthening E-cadherin cell-cell adhesions and mechanosensing. (ebi.ac.uk)
  • β-Catenin is essential for embryonic development and required for cell renewal/regeneration in adult life. (springer.com)
  • 2002 Beta-catenin and TCF mediate cell positioning in the intestinal epithelium by controlling the expression of EphB/ephrinB. (springer.com)
  • 2004 Synergy between LRH-1 and β-catenin induces G1 cyclin-mediated cell proliferation. (springer.com)
  • Wnt-1 signaling inhibits apoptosis by activating β -catenin/T cell factor-mediated transcription," Journal of Cell Biology , vol. 152, no. 1, pp. 87-96, 2001. (hindawi.com)
  • however, the relative contribution of β-catenin activity and function in specific cell types in the granulation tissue during the healing process is unknown. (jci.org)
  • Treatment of a primary PC cell culture with the DNA hypomethylating agent 5-aza-2'-deoxycytidine (decitabine, Dacogen(r)) induced APC expression, reduced active nonphosphorylated β-catenin, inhibited cell growth, and caused apoptosis. (sigmaaldrich.com)
  • Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. (thermofisher.com)
  • Confocal microscopic image of β‐catenin in a four‐cell Xenopus embryo. (els.net)
  • Miller JR and Moon RT (1996) Signal transduction through β‐catenin and specification of cell fate during embryogenesis. (els.net)
  • Furthermore, exogenous β-catenin mediated modest cell survival and rendered cells sensitive to apoptotic stimuli. (oregonstate.edu)
  • However, a separate report demonstrated that loss of β-catenin did not affect pancreatic endocrine cell mass, despite the almost complete loss of the exocrine compartment. (biologists.org)
  • IWP-2 is an inhibitor of Wnt processing and secretion with IC50 of 27 nM in a cell-free assay, selective blockage of Porcn-mediated Wnt palmitoylation, does not affect Wnt/β-catenin in general and displays no effect against Wnt-stimulated cellular responses. (selleckchem.com)
  • However, it is not known whether Wnt signaling in B cell development is mediated by β-catenin and whether β-catenin plays a role in mature B cell function. (jimmunol.org)
  • Thus, β-catenin expression was found to be dispensable for normal B cell development and function. (jimmunol.org)
  • The anti-β-catenin nanobodies and chromobodies characterized in this study are versatile tools that enable a novel and unique approach to monitor the dynamics of subcellular β-catenin in biochemical and cell biological assays. (mcponline.org)
  • In contrast, sequestration of cytosolic αE-catenin to the plasma membrane reduces membrane dynamics. (rupress.org)
  • Short hairpin RNA-mediated knockdown of Wnt5a , but not treatment with Dkk1, an antagonist of Wnt/β-catenin signaling, reduced the expression of Lrp5 and Lrp6 in osteoblast-lineage cells under osteogenic culture conditions. (nature.com)
  • β-catenin was a crucial mediator of the anti-adipogenic effects of shikonin, as determined by siRNA-mediated knockdown. (biomedsearch.com)
  • Over the past two decades, interdisciplinary research has tremendously advanced our knowledge of β-catenin function and its involvement in human disorders (Takemaru et al. (springer.com)
  • Molecular cloning reveals alternative splice forms of human alpha(E)-catenin. (ebi.ac.uk)
  • Heterogeneous sensitivity of human acute myeloid leukemia to β -catenin down-modulation," Leukemia , vol. 25, no. 5, pp. 770-780, 2011. (hindawi.com)
  • Synthetic peptide, corresponding to a region within amino acids 275-285 of Human gamma Catenin. (abcam.com)
  • Here we show a cross-talk between β-catenin and ERα in human breast cancer cells. (mdpi.com)
  • Human endometrial adenocarcinoma: immunohistochemical staining for beta-catenin using NCL-B-CAT. (leicabiosystems.com)
  • To date, β-catenin has been reported to be implicated in mediating the epithelial-mesenchymal transition (EMT) in a variety of human cancers, which can be triggered by EGF. (dovepress.com)
  • Specificity Recognizes human Catenin, beta. (gentaur.com)
  • Interestingly, GPCs from AD patients exhibited elevated levels of glycogen synthase kinase 3β (GSK-3β, an enzyme known to phosphorylate β-catenin), accompanied by an increase in phosphorylated β-catenin and a decrease in nonphosphorylated β-catenin compared with HC counterparts. (jneurosci.org)
  • Takemaru K-I, Ohmitsu M, Li F-Q. An oncogenic hub: beta-catenin as a molecular target for cancer therapeutics. (springer.com)
  • As the authors write, "These findings suggest that the affinity of β-catenin for cadherin initially weakens to allow for modifications of the synapse, and then strengthens to stabilize the synapse, which we hypothesize to be a molecular and cellular correlate of memory consolidation. (alzforum.org)