Catechols: A group of 1,2-benzenediols that contain the general formula R-C6H5O2.Catechol 2,3-Dioxygenase: Catalyzes the oxidation of catechol to 2-hydroxymuconate semialdehyde in the carbazole and BENZOATE degradation via HYDROXYLATION pathways. It also catalyzes the conversion of 3-methylcatechol to cis, cis-2-hydroxy-6-oxohept-2,4-dienoate in the TOLUENE and XYLENE degradation pathway. This enzyme was formerly characterized as EC 1.13.1.2.Dioxygenases: Non-heme iron-containing enzymes that incorporate two atoms of OXYGEN into the substrate. They are important in biosynthesis of FLAVONOIDS; GIBBERELLINS; and HYOSCYAMINE; and for degradation of AROMATIC HYDROCARBONS.Catechol 1,2-Dioxygenase: An enzyme that catalyzes the oxidation of catechol to muconic acid with the use of Fe3+ as a cofactor. This enzyme was formerly characterized as EC 1.13.1.1 and EC 1.99.2.2.Oxygenases: Oxidases that specifically introduce DIOXYGEN-derived oxygen atoms into a variety of organic molecules.Estrogens, Catechol: 2- or 4-Hydroxyestrogens. Substances that are physiologically active in mammals, especially in the control of gonadotropin secretion. Physiological activity can be ascribed to either an estrogenic action or interaction with the catecholaminergic system.4-Hydroxyphenylpyruvate Dioxygenase: An enzyme that catalyzes the conversion of 4-hydroxyphenylpyruvate plus oxygen to homogentisic acid and carbon dioxide. EC 1.13.11.27.Protocatechuate-3,4-Dioxygenase: An enzyme that catalyzes the oxidation of protocatechuate to 3-carboxy-cis-cis-muconate in the presence of molecular oxygen. It contains ferric ion. EC 1.13.11.3.Cysteine Dioxygenase: An enzyme that catalyzes the conversion of L-CYSTEINE to 3-sulfinoalanine (3-sulfino-L-alanine) in the CYSTEINE metabolism and TAURINE and hypotaurine metabolic pathways.Tryptophan Oxygenase: A dioxygenase with specificity for the oxidation of the indoleamine ring of TRYPTOPHAN. It is a LIVER-specific enzyme that is the first and rate limiting enzyme in the kynurenine pathway of TRYPTOPHAN catabolism.Catechol O-Methyltransferase: Enzyme that catalyzes the movement of a methyl group from S-adenosylmethionone to a catechol or a catecholamine.Homogentisate 1,2-Dioxygenase: A mononuclear Fe(II)-dependent oxygenase, this enzyme catalyzes the conversion of homogentisate to 4-maleylacetoacetate, the third step in the pathway for the catabolism of TYROSINE. Deficiency in the enzyme causes ALKAPTONURIA, an autosomal recessive disorder, characterized by homogentisic aciduria, OCHRONOSIS and ARTHRITIS. This enzyme was formerly characterized as EC 1.13.1.5 and EC 1.99.2.5.3-Hydroxyanthranilate 3,4-Dioxygenase: An enzyme that catalyzes the conversion of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde. It was formerly characterized as EC 1.13.1.6.Pseudomonas: A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.KynureninePseudomonas putida: A species of gram-negative, aerobic bacteria isolated from soil and water as well as clinical specimens. Occasionally it is an opportunistic pathogen.Hydroxybenzoates: Benzoate derivatives substituted by one or more hydroxy groups in any position on the benzene ring.Rhodococcus: A bacterial genus of the order ACTINOMYCETALES.Phenol: An antiseptic and disinfectant aromatic alcohol.ChlorobenzenesSorbic Acid: Mold and yeast inhibitor. Used as a fungistatic agent for foods, especially cheeses.Catechol Oxidase: An enzyme of the oxidoreductase class that catalyzes the reaction between catechol and oxygen to yield benzoquinone and water. It is a complex of copper-containing proteins that acts also on a variety of substituted catechols. EC 1.10.3.1.Tryptophan: An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals.Chlorobenzoates: Benzoic acid or benzoic acid esters substituted with one or more chlorine atoms.Toluene: A widely used industrial solvent.Benzoates: Derivatives of BENZOIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxybenzene structure.Mixed Function Oxygenases: Widely distributed enzymes that carry out oxidation-reduction reactions in which one atom of the oxygen molecule is incorporated into the organic substrate; the other oxygen atom is reduced and combined with hydrogen ions to form water. They are also known as monooxygenases or hydroxylases. These reactions require two substrates as reductants for each of the two oxygen atoms. There are different classes of monooxygenases depending on the type of hydrogen-providing cosubstrate (COENZYMES) required in the mixed-function oxidation.Sphingomonas: A genus of gram-negative, aerobic, rod-shaped bacteria characterized by an outer membrane that contains glycosphingolipids but lacks lipopolysaccharide. They have the ability to degrade a broad range of substituted aromatic compounds.Burkholderia: A genus of gram-negative, aerobic, rod-shaped bacteria. Organisms in this genus had originally been classified as members of the PSEUDOMONAS genus but overwhelming biochemical and chemical findings indicated the need to separate them from other Pseudomonas species, and hence, this new genus was created.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Benzoic Acid: A fungistatic compound that is widely used as a food preservative. It is conjugated to GLYCINE in the liver and excreted as hippuric acid.Gentisates: Salts and esters of gentisic acid.Alcaligenes: A genus of gram-negative, aerobic, motile bacteria that occur in water and soil. Some are common inhabitants of the intestinal tract of vertebrates. These bacteria occasionally cause opportunistic infections in humans.Benzene: Toxic, volatile, flammable liquid hydrocarbon byproduct of coal distillation. It is used as an industrial solvent in paints, varnishes, lacquer thinners, gasoline, etc. Benzene causes central nervous system damage acutely and bone marrow damage chronically and is carcinogenic. It was formerly used as parasiticide.Adipates: Derivatives of adipic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a 1,6-carboxy terminated aliphatic structure.Equilenin: An estrogenic steroid produced by HORSES. It has a total of five double bonds in the A- and B-ring. High concentration of equilenin is found in the URINE of pregnant mares.Comamonas: A genus of gram-negative, straight or slightly curved rods which are motile by polar flagella and which accumulate poly-beta-hydroxybutyrate within the cells.Genes, Bacterial: The functional hereditary units of BACTERIA.Homogentisic AcidOxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Acinetobacter: A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.Pyrogallol: A trihydroxybenzene or dihydroxy phenol that can be prepared by heating GALLIC ACID.Brevibacterium: A gram-positive organism found in dairy products, fresh and salt water, marine organisms, insects, and decaying organic matter.Hydrocarbons, Aromatic: Organic compounds containing carbon and hydrogen in the form of an unsaturated, usually hexagonal ring structure. The compounds can be single ring, or double, triple, or multiple fused rings.Phenols: Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.Ralstonia: A genus in the family BURKHOLDERIACEAE, comprised of many species. They are associated with a variety of infections including MENINGITIS; PERITONITIS; and URINARY TRACT INFECTIONS.HydroquinonesAmino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Planococcus Bacteria: A genus of coccoid bacteria in the family PLANOCOCCACEAE. They are widely distributed in various habitats including sea water, freshwater ponds, cyanobacterial mats, and in marine animals.Iron: A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.Hydroxylation: Placing of a hydroxyl group on a compound in a position where one did not exist before. (Stedman, 26th ed)Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Parabens: Methyl, propyl, butyl, and ethyl esters of p-hydroxybenzoic acid. They have been approved by the FDA as antimicrobial agents for foods and pharmaceuticals. (From Hawley's Condensed Chemical Dictionary, 11th ed, p872)Polychlorinated Biphenyls: Industrial products consisting of a mixture of chlorinated biphenyl congeners and isomers. These compounds are highly lipophilic and tend to accumulate in fat stores of animals. Many of these compounds are considered toxic and potential environmental pollutants.Gram-Negative Aerobic Rods and Cocci: A group of gram-negative bacteria consisting of rod- and coccus-shaped cells. They are both aerobic (able to grow under an air atmosphere) and microaerophilic (grow better in low concentrations of oxygen) under nitrogen-fixing conditions but, when supplied with a source of fixed nitrogen, they grow as aerobes.Ferredoxins: Iron-containing proteins that transfer electrons, usually at a low potential, to flavoproteins; the iron is not present as in heme. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Kinetics: The rate dynamics in chemical or physical systems.CresolsBurkholderiaceae: A family of gram negative, aerobic, non-sporeforming, rod-shaped bacteria.3-Hydroxyanthranilic Acid: An oxidation product of tryptophan metabolism. It may be a free radical scavenger and a carcinogen.gamma-Butyrobetaine Dioxygenase: An enzyme that catalyzes the HYDROXYLATION of gamma-butyrobetaine to L-CARNITINE. It is the last enzyme in the biosynthetic pathway of L-CARNITINE and is dependent on alpha-ketoglutarate; IRON; ASCORBIC ACID; and OXYGEN.Arthrobacter: A genus of asporogenous bacteria isolated from soil that displays a distinctive rod-coccus growth cycle.Alkaptonuria: An inborn error of amino acid metabolism resulting from a defect in the enzyme HOMOGENTISATE 1,2-DIOXYGENASE, an enzyme involved in the breakdown of PHENYLALANINE and TYROSINE. It is characterized by accumulation of HOMOGENTISIC ACID in the urine, OCHRONOSIS in various tissues, and ARTHRITIS.Xylenes: A family of isomeric, colorless aromatic hydrocarbon liquids, that contain the general formula C6H4(CH3)2. They are produced by the destructive distillation of coal or by the catalytic reforming of petroleum naphthenic fractions. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Ketoglutaric Acids: A family of compounds containing an oxo group with the general structure of 1,5-pentanedioic acid. (From Lehninger, Principles of Biochemistry, 1982, p442)Biphenyl CompoundsSalicylates: The salts or esters of salicylic acids, or salicylate esters of an organic acid. Some of these have analgesic, antipyretic, and anti-inflammatory activities by inhibiting prostaglandin synthesis.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Intramolecular Lyases: Enzymes of the isomerase class that catalyze reactions in which a group can be regarded as eliminated from one part of a molecule, leaving a double bond, while remaining covalently attached to the molecule. (From Enzyme Nomenclature, 1992) EC 5.5.Ferrous Compounds: Inorganic or organic compounds that contain divalent iron.ToluidinesEnzyme Induction: An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.ortho-Aminobenzoates: Benzoic acids, salts, or esters that contain an amino group attached to carbon number 2 or 6 of the benzene ring structure.Vanillic Acid: A flavoring agent. It is the intermediate product in the two-step bioconversion of ferulic acid to vanillin. (J Biotechnol 1996;50(2-3):107-13).Biotransformation: The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.Bacterial Proteins: Proteins found in any species of bacterium.Sulfanilic Acids: Aminobenzenesulfonic acids. Organic acids that are used in the manufacture of dyes and organic chemicals and as reagents.PhenanthrenesBase Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Iron-Sulfur Proteins: A group of proteins possessing only the iron-sulfur complex as the prosthetic group. These proteins participate in all major pathways of electron transport: photosynthesis, respiration, hydroxylation and bacterial hydrogen and nitrogen fixation.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Naphthalenes: Two-ring crystalline hydrocarbons isolated from coal tar. They are used as intermediates in chemical synthesis, as insect repellents, fungicides, lubricants, preservatives, and, formerly, as topical antiseptics.Benzene DerivativesHydroxyestrones: Estrone derivatives substituted with one or more hydroxyl groups in any position. They are important metabolites of estrone and other estrogens.Quinolinic Acid: A metabolite of tryptophan with a possible role in neurodegenerative disorders. Elevated CSF levels of quinolinic acid are correlated with the severity of neuropsychological deficits in patients who have AIDS.Spectrophotometry: The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.Pseudomonas stutzeri: A species of gram-negative bacteria in the genus PSEUDOMONAS, containing multiple genomovars. It is distinguishable from other pseudomonad species by its ability to use MALTOSE and STARCH as sole carbon and energy sources. It can degrade ENVIRONMENTAL POLLUTANTS and has been used as a model organism to study denitrification.Delftia acidovorans: A species of gram-negative rod-shaped bacteria found ubiquitously and formerly called Comamonas acidovorans and Pseudomonas acidovorans. It is the type species of the genus DELFTIA.Electron Spin Resonance Spectroscopy: A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.Chlorophenols: Phenols substituted with one or more chlorine atoms in any position.Acinetobacter calcoaceticus: A species of gram-negative, aerobic bacteria found in soil and water. Although considered to be normally nonpathogenic, this bacterium is a causative agent of nosocomial infections, particularly in debilitated individuals.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Oxidoreductases: The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)3,4-Dihydroxyphenylacetic Acid: A deaminated metabolite of LEVODOPA.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Multigene Family: A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)Burkholderia cepacia: A species of BURKHOLDERIA considered to be an opportunistic human pathogen. It has been associated with various types of infections of nosocomial origin.Oxygen: An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.Hydrolases: Any member of the class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting molecules, e.g., ESTERASES, glycosidases (GLYCOSIDE HYDROLASES), lipases, NUCLEOTIDASES, peptidases (PEPTIDE HYDROLASES), and phosphatases (PHOSPHORIC MONOESTER HYDROLASES). EC 3.beta-Carotene 15,15'-Monooxygenase: A monooxygenase that catalyzes the conversion of BETA-CAROTENE into two molecules of RETINAL. It was formerly characterized as EC 1.13.11.21 and EC 1.18.3.1.Oxygen Isotopes: Stable oxygen atoms that have the same atomic number as the element oxygen, but differ in atomic weight. O-17 and 18 are stable oxygen isotopes.Enzyme Reactivators: Compounds which restore enzymatic activity by removing an inhibitory group bound to the reactive site of the enzyme.Tyrosinemias: A group of disorders which have in common elevations of tyrosine in the blood and urine secondary to an enzyme deficiency. Type I tyrosinemia features episodic weakness, self-mutilation, hepatic necrosis, renal tubular injury, and seizures and is caused by a deficiency of the enzyme fumarylacetoacetase. Type II tyrosinemia features INTELLECTUAL DISABILITY, painful corneal ulcers, and keratoses of the palms and plantar surfaces and is caused by a deficiency of the enzyme TYROSINE TRANSAMINASE. Type III tyrosinemia features INTELLECTUAL DISABILITY and is caused by a deficiency of the enzyme 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE. (Menkes, Textbook of Child Neurology, 5th ed, pp42-3)Naphthols: Naphthalene derivatives carrying one or more hydroxyl (-OH) groups at any ring position. They are often used in dyes and pigments, as antioxidants for rubber, fats, and oils, as insecticides, in pharmaceuticals, and in numerous other applications.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Catalysis: The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.2,4-Dichlorophenoxyacetic Acid: An herbicide with irritant effects on the eye and the gastrointestinal system.Stereoisomerism: The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)MaleatesSoil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.Trichloroethylene: A highly volatile inhalation anesthetic used mainly in short surgical procedures where light anesthesia with good analgesia is required. It is also used as an industrial solvent. Prolonged exposure to high concentrations of the vapor can lead to cardiotoxicity and neurological impairment.Pseudomonas pseudoalcaligenes: A species of gram-negative bacteria in the genus PSEUDOMONAS. All strains can utilize FRUCTOSE for energy. It is occasionally isolated from humans and some strains are pathogenic to WATERMELON.Aniline CompoundsSiderophores: Low-molecular-weight compounds produced by microorganisms that aid in the transport and sequestration of ferric iron. (The Encyclopedia of Molecular Biology, 1994)Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Spectrophotometry, Ultraviolet: Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Quinones: Hydrocarbon rings which contain two ketone moieties in any position. They can be substituted in any position except at the ketone groups.Gram-Negative Aerobic Bacteria: A large group of aerobic bacteria which show up as pink (negative) when treated by the gram-staining method. This is because the cell walls of gram-negative bacteria are low in peptidoglycan and thus have low affinity for violet stain and high affinity for the pink dye safranine.Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Monophenol Monooxygenase: An enzyme of the oxidoreductase class that catalyzes the reaction between L-tyrosine, L-dopa, and oxygen to yield L-dopa, dopaquinone, and water. It is a copper protein that acts also on catechols, catalyzing some of the same reactions as CATECHOL OXIDASE. EC 1.14.18.1.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Isomerases: A class of enzymes that catalyze geometric or structural changes within a molecule to form a single product. The reactions do not involve a net change in the concentrations of compounds other than the substrate and the product.(from Dorland, 28th ed) EC 5.Gallic Acid: A colorless or slightly yellow crystalline compound obtained from nutgalls. It is used in photography, pharmaceuticals, and as an analytical reagent.1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt: A colorimetric reagent for iron, manganese, titanium, molybdenum, and complexes of zirconium. (From Merck Index, 11th ed)Pseudomonas mendocina: A species of gram-negative bacteria in the genus PSEUDOMONAS, which is found in SOIL and WATER.Soil Pollutants: Substances which pollute the soil. Use for soil pollutants in general or for which there is no specific heading.Benzofurans: Compounds that contain a BENZENE ring fused to a furan ring.Kynurenine 3-Monooxygenase: An NADPH-dependent flavin monooxygenase that plays a key role in the catabolism of TRYPTOPHAN by catalyzing the HYDROXYLATION of KYNURENINE to 3-hydroxykynurenine. It was formerly characterized as EC 1.14.1.2 and EC 1.99.1.5.Interferon-gamma: The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.Nonheme Iron Proteins: Proteins, usually acting in oxidation-reduction reactions, containing iron but no porphyrin groups. (Lehninger, Principles of Biochemistry, 1993, pG-10)Adrenochrome: Pigment obtained by the oxidation of epinephrine.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Multienzyme Complexes: Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Models, Chemical: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.BenzophenonesSequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Gas Chromatography-Mass Spectrometry: A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Normetanephrine: A methylated metabolite of norepinephrine that is excreted in the urine and found in certain tissues. It is a marker for tumors.Carboxy-Lyases: Enzymes that catalyze the addition of a carboxyl group to a compound (carboxylases) or the removal of a carboxyl group from a compound (decarboxylases). EC 4.1.1.Comamonadaceae: A family of gram-negative aerobic bacteria in the class BETA PROTEOBACTERIA, encompassing the acidovorans rRNA complex. Some species are pathogenic for PLANTS.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Taurine: A conditionally essential nutrient, important during mammalian development. It is present in milk but is isolated mostly from ox bile and strongly conjugates bile acids.
Junca, H.; Plumeier, I.; Hecht, H. J. R.; Pieper, D. H. (December 2004). "Difference in kinetic behaviour of catechol 2,3- ... dioxygenase variants from a polluted environment". Microbiology. 150 (12): 4181-4187. doi:10.1099/mic.0.27451-0. PMID 15583170 ... 227 (1-2): 21-30. doi:10.1016/j.mce.2004.08.001. PMID 15501581. Görg, A.; Weiss, W.; Dunn, M. J. (December 2004). "Current two- ... In recent years, 2-D electrophoresis has been widely accepted as a standard procedure to separate complex protein mixtures in ...
The isofunctional enzymes of catechol 1,2-dioxygenase from species of Acinetobacter, Pseudomonas, Nocardia, Alcaligenes and ... Extradiol Cleavage of 3-Methylcatechol by Catechol 1,2-Dioxygenase from Various Microorganisms. C. T. Hou, R. Patel and M. O. ... The 3-methylcatechol structural motif is rare in natural products. Known examples include calopin and a δ-lactone derivative, O ... 3-Methylcatechol is a chemical compound. The enzyme 1,2-dihydroxy-6-methylcyclohexa-3,5-dienecarboxylate dehydrogenase uses 1,2 ...
Sala-Trepat JM, Evans WC (1971). "The meta cleavage of catechol by Azotobacter species 4-Oxalocrotonate pathway". Eur. J. ... 2-Hydroxymuconate semialdehyde is formed from catechol by the enzyme catechol 2,3-dioxygenase during the degradation of ... It is hydrolysed into formate and 2-oxopent-4-enoate by 2-hydroxymuconate-semialdehyde hydrolase. ... 20 (3): 400-13. doi:10.1111/j.1432-1033.1971.tb01406.x. PMID 4325686. ...
... catechol 2,3-dioxygenase), and is an intermediate in the bacterial degradation of several aromatic compounds. Inoue, J.; Shaw, ... 2-Hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85, xylG (gene), praB (gene)) is an enzyme with systematic name (2E,4Z ... 2-hydroxymuconate-6-semialdehyde dehydrogenase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular ... Orii, C.; Takenaka, S.; Murakami, S.; Aoki, K. (2006). "Metabolism of 4-amino-3-hydroxybenzoic acid by Bordetella sp. strain ...
4-dioxygenase uses 3,4-dihydroxybenzoate and O2 to produce 3-carboxy-cis,cis-muconate. Ethyl protocatechuate Dawson, R. M. C. ... 4-dihydroxybenzoate to produce catechol and CO2. The enzyme protocatechuate 3, ... 4-dioxygenase Vanillate monooxygenase 3,4-dihydroxyphthalate decarboxylase 4,5-dihydroxyphthalate decarboxylase Degradation ... 23 (2): 201-208. doi:10.1016/j.tiv.2008.11.008. PMID 19095056. Anter J, Romero-Jiménez M, Fernández-Bedmar Z, Villatoro-Pulido ...
... catechol 2,3-dioxygenase, 3,4-dihydroxyphenylacetate 2,3-dioxygenase and 4-hydroxyphenylpyruvate dioxygenase all resemble ... 4 (3): e4649. doi:10.1371/journal.pone.0004649. PMC 2650792 . PMID 19266052. Distler MG, Plant LD, Sokoloff G, Hawk AJ, Aneas I ... 190 (2): 685-96. PMID 14841219. Allen RE, Lo TW, Thornalley PJ (1993). "A simplified method for the purification of human red ... 221 (2): 297-315. doi:10.1007/s00213-011-2574-z. PMC 3337404 . PMID 22113448. Thornalley PJ (May 2006). "Unease on the role of ...
"Characterization of an O2 Adduct of an Active Cobalt-Substituted Extradiol-Cleaving Catechol Dioxygenase". Journal of the ... "A Hyperactive Cobalt-Substituted Extradiol-Cleaving Catechol Dioxygenase". Journal of biological inorganic chemistry : JBIC : a ... which mediates the electron transfer between catechol substrates and O2 to form a [M(II)(semiquinone)superoxo] intermediate. ... 13 (2): 117-126. ISSN 0006-2944. PMID 1. Bose, K. S.; Sarma, R. H. (1975-10-27). "Delineation of the intimate details of the ...
... dependent dioxygenases. The catechol dioxygenases, some of the most well-studied dioxygenase enzymes, use dioxygen to cleave a ... Two important groups of mononuclear, non-heme iron dioxygenases are catechol dioxygenases and 2-oxoglutarate (2OG)- ... Catechol dioxygenases are further classified as being "extradiol" or "intradiol," and this distinction is based on mechanistic ... In this respect, these enzymes are reminiscent of the intradiol catechol dioxygenases whereby the metal centers activate the ...
Catechol dioxygenases are metalloprotein enzymes that carry out the oxidative cleavage of catechols. This class of enzymes ... 4-dioxygenase (EC 1.13.11.3). The active site of catechol dioxygenases most frequently contains iron, but manganese-containing ... Catechol dioxygenases belong to the class of oxidoreductases and have several different substrate specificities, including ... An example of the reaction carried out by catechol 1,2-dioxygenase is the formation of cis,cis-muconic acid from catechol, ...
Muller R, Schmitt S, Lingens F (1982). "A novel non-heme iron-containing dioxygenase. Chloridazon-catechol dioxygenase from ... Chloridazon-catechol dioxygenase (EC 1.13.11.36) is an enzyme that catalyzes the chemical reaction 5-amino-4-chloro-2-(2,3- ... Muller R, Haug S, Eberspacher J, Lingens F (1977). "[Catechol 2,3-dioxygenase from pyrazon-degrading bacteria (author's transl ... 125 (3): 579-84. doi:10.1111/j.1432-1033.1982.tb06722.x. PMID 6811270. Molecular and Cellular Biology portal. ...
Junca, H.; Plumeier, I.; Hecht, H. J. R.; Pieper, D. H. (December 2004). "Difference in kinetic behaviour of catechol 2,3- ... dioxygenase variants from a polluted environment". Microbiology. 150 (12): 4181-4187. doi:10.1099/mic.0.27451-0. PMID 15583170. ... 2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] and a crucial step in gene expression profiling. ... 2 (3): 295-306. doi:10.1586/14789450.2.3.295. PMID 16000077.. *^ Piette, André; Derouaux, Adeline; Gerkens, Pascal; Noens, Elke ...
... a family of catechol dioxygenases that cleaves the bond between the phenolic hydroxyl groups of catechol using an Fe3+ cofactor ... catechol with catechol dioxygenases". Arch. Biochem. Biophys. 248 (7): 130-137. doi:10.1016/0003-9861(86)90409-1. PMID 3015028 ... Two families of dioxygenases were discovered by Osamu Hayaishi and Kizo Hashimoto in 1950: catechol 1,2-dioxygenase and ... catechol dioxygenase, pyrocatechase, pyrocatechol 1,2-dioxygenase, CD I, CD II) is an enzyme that catalyzes the oxidative ring ...
... at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and Cellular Biology ... Catechol 2,3-dioxygenase (EC 1.13.11.2, 2,3-pyrocatechase, catechol 2,3-oxygenase, catechol oxygenase, metapyrocatechase, ... Kojima, Y.; Itada, N.; Hayaishi, O. (1961). "Metapyrocatechase: a new catechol-cleaving enzyme". J. Biol. Chem. 236: 2223-2228 ... is an enzyme with systematic name catechol:oxygen 2,3-oxidoreductase (decyclizing). This enzyme catalyses the following ...
Kobayashi S, Hayaishi O (1970). "Anthranilic acid conversion to catechol (Pseudomonas)". Methods Enzymol. 17A: 505-510. doi: ... catechol + CO2 + NAD(P)+ + NH3 The 5 substrates of this enzyme are anthranilate, NADH, NADPH, H+, and O2, whereas its 5 ... "Enzymatic formation of catechol from anthranilic acid". J. Biol. Chem. 239: 2204-2211. PMID 14209949. Molecular and Cellular ... products are catechol, CO2, NAD+, NADP+, and NH3. This enzyme belongs to the family of oxidoreductases, specifically those ...
I Enzymatic formation of catechol from benzene". Biochemistry. 7 (7): 2653-62. doi:10.1021/bi00847a031. PMID 4298226. Molecular ... and benzene dioxygenase. This enzyme participates in naphthalene and anthracene degradation. It has 4 cofactors: FAD, Iron, ... In enzymology, a benzene 1,2-dioxygenase (EC 1.14.12.3) is an enzyme that catalyzes the chemical reaction benzene + NADH + H+ ... The systematic name of this enzyme class is benzene,NADH:oxygen oxidoreductase (1,2-hydroxylating). Other names in common use ...
... dioxygenases MeSH D08.811.682.690.416.277 --- catechol 1,2-dioxygenase MeSH D08.811.682.690.416.305 --- catechol 2,3- ... dioxygenase MeSH D08.811.682.690.416.319 --- cysteine dioxygenase MeSH D08.811.682.690.416.326 --- homogentisate 1,2- ... 4-dioxygenase MeSH D08.811.682.690.416.330 --- 4-hydroxyphenylpyruvate dioxygenase MeSH D08.811.682.690.416.333 --- indoleamine ... catechol oxidase MeSH D08.811.682.690.708.125.500 --- monophenol monooxygenase MeSH D08.811.682.690.708.170 --- cytochrome p- ...
... related with the catechol and resorcinol groups and the oxidation is pH-dependent. The oxidation of the catechol 3',4'- ... 4-dioxygenase and hydroxyquinol 1,2-dioxygenase to form β-carboxy cis, cis-muconic acid and maleyl acetate. Among fungi, ... sulphation and methylation of the catechol group by catechol-O-methyl transferase, with only small amounts detected in plasma. ... The A ring is similar to a resorcinol moiety while the B ring is similar to a catechol moiety. There are two chiral centers on ...
Catechol dioxygenases. Department of Chemistry, Michigan State University, East Lansing, MI 488241322, U.S.A. ... Diversity of catechol 2,3-dioxygenase genes of bacteria responding to dissolved organic matter derived from different sources ... PCR isolation of catechol 2,3-dioxygenase gene fragments from environmental samples and their assembly into functional genes. ... The Catechol 1,2 Dioxygenase System of Acinetobacter radioresistens: Isoenzymes, Inductors and Gene localisation. Biol. Chem., ...
EC 2.1.1 Catechol-O-methyl transferase EC 2.1.1.6 DNA methyltransferase EC 2.1.1.72, EC 2.1.1.113, EC 2.1.1.37 Histone ... 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) Category:EC 1.13.12 (With incorporation of one atom of oxygen (internal ... Catechol oxidase EC 1.10.3.1 Laccase EC 1.10.3.2 Category:EC 1.10.99 (with other acceptors) Category:EC 1.11.1 (peroxidases) ... EC 1.14.12 Nitric oxide dioxygenase Category:EC 1.14.13 Nitric oxide synthase EC 1.14.13.39 Category:EC 1.14.14 Aromatase EC ...
"The genes coding for the conversion of carbazole to catechol are flanked by IS6100 elements in Sphingomonas sp. strain XLDN2-5 ... 9a-dioxygenase, a three-component dioxygenase system of Pseudomonas resinovorans strain CA10". Appl. Environ. Microbiol. 68 (12 ... Carbazole 1,9a-dioxygenase (EC 1.14.12.22, CARDO) is an enzyme with systematic name 9H-carbazole,NAD(P)H:oxygen oxidoreductase ... Carbazole 1,9a-dioxygenase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and Cellular ...
O-rhamnoside 7-O-methyltransferase Catechol-O-methyl transferase 5-desoxy-malvidin Capensinidin Europinidin Hirsutidin Malvidin ... and evidence that partially methylated flavanones are substrates of four different flavonoid dioxygenases". Phytochemistry. 65 ... 119 (2): 155-62. doi:10.1016/j.jbiotec.2005.04.004. PMID 15961179. Schroder, G; Wehinger, E; Lukacin, R; Wellmann, F; Seefelder ... Those positions can be ortho, meta, para and there can be a special 3-O-methyltransferase for the 3-OH position. Calamondin ...
Heating catechin past its point of decomposition releases pyrocatechol (also called catechol), which explains the common origin ... leucoanthocyanidin dioxygenase), E11, anthocyanidin reductase. HSCoA, Coenzyme A. L-Tyr, L-tyrosine, L-Phe, L-phenylalanine. ... Flavan-3-ols (sometimes referred to as flavanols) are derivatives of flavans that use the 2-phenyl-3,4-dihydro-2H-chromen-3-ol ... 8 (2): 1489. doi:10.2903/j.efsa.2010.1489. CS1 maint: Multiple names: authors list (link) "Inspections, Compliance, Enforcement ...
For example, Rhodococci expresses dioxygenases, which can be used to degrade benzotrifluoride, a recalcitrant pollutant. ... catechol, benzoate, and protocatechuic acid. Rhodococci are also capable of accumulating heavy metal ions, such as radioactive ... "Degradation of benzotrifluoride via the dioxygenase pathway in Rhodococcus sp. 065240". Bioscience, Biotechnology, and ... 78 (2-3): 221-226. ISSN 0378-1097. PMID 1490602. Parekh, N. R.; Walker, A.; Roberts, S. J.; Welch, S. J. (November 1994). " ...
other dioxygenase: Catechol dioxygenase. *Homogentisate 1,2-dioxygenase. *Cysteine dioxygenase. *4-Hydroxyphenylpyruvate ... ALOX5's dioxygenase activity adds a hydroperoxyl (i.e. HO2) residue to arachidonic acid (i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic ... dioxygenase activity. • metal ion binding. • protein binding. • oxidoreductase activity. • oxidoreductase activity, acting on ... 39 (1): 3-22. doi:10.1002/cbin.10345. PMID 25052386.. *^ a b Barden AE, Mas E, Mori TA (2016). "n-3 Fatty acid supplementation ...
... chloridazon-catechol dioxygenase EC 1.13.11.37: hydroxyquinol 1,2-dioxygenase EC 1.13.11.38: 1-hydroxy-2-naphthoate 1,2- ... sulfur dioxygenase EC 1.13.11.19: cysteamine dioxygenase EC 1.13.11.20: cysteine dioxygenase EC 1.13.11.21: now *EC 1.14.99.36 ... gibberellin 3b-dioxygenase EC 1.14.11.16: peptide-aspartate b-dioxygenase EC 1.14.11.17: taurine dioxygenase EC 1.14.11.18: ... dioxygenase EC 1.14.11.11: hyoscyamine (6S)-dioxygenase EC 1.14.11.12: gibberellin-44 dioxygenase EC 1.14.11.13: gibberellin 2b ...
The meta-hydroxyl group of catechol is methylated by catechol-O-methyltransferase. Four of the five hydroxyl groups of ... 2 (5): 235-44. doi:10.1039/c1fo10025d. PMC 4122511. PMID 21779561.. *^ a b Day AJ, Rothwell JA, Morgan R (2004). " ... 60 (3): 595-602. PMID 11502892.. *^ van der Woude H, Ter Veld MG, Jacobs N, van der Saag PT, Murk AJ, Rietjens IM (2005). "The ... 6 (3): 173-93. PMID 17724002.. *^ Maggiolini M, Bonofiglio D, Marsico S, Panno ML, Cenni B, Picard D, Andò S (2001). "Estrogen ...
In the absence of a suitable substrate for the dioxygenase, ThnA3 would accumulate in its reduced form and act as a negative ... is responsible for the extradiol cleavage of the catechol derivative (1), followed by hydrolytic cleavage (by ThnD) of the C-C ... Identification of an extradiol dioxygenase involved in tetralin biodegradation: gene sequence analysis, purification, and ... Biotechnol. 2:262-273. [PubMed]. 37. Toth, J., A. A. Ismaiel, and J.-S. Chen. 1999. The ald gene, encoding a coenzyme A- ...
4-dioxygenase, a novel eukaryotic homolog in the degradation pathway of 2-nitrobenzoate by Pseudomonas fluorescens strain KU-7そ ... or catechol were detected as intermediates in other bacteria.Crude extracts of KU-7 cells converted 2-nitrobenzoate to 3- ... Lett.327(2),142-1472012年1月 学会発表海洋性フタル酸資化性菌の分離と解析査読無その他共著西村彩香(D);岩木宏明;長谷川喜衛;;日本防菌防黴学会 第 38 回年
Catechol 2,3-dioxygenase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and Cellular Biology ... Catechol 2,3-dioxygenase (EC 1.13.11.2, 2,3-pyrocatechase, catechol 2,3-oxygenase, catechol oxygenase, metapyrocatechase, ... Kojima, Y.; Itada, N.; Hayaishi, O. (1961). "Metapyrocatechase: a new catechol-cleaving enzyme". J. Biol. Chem. 236: 2223-2228 ... is an enzyme with systematic name catechol:oxygen 2,3-oxidoreductase (decyclizing). This enzyme catalyses the following ...
What is catechol 2,3-dioxygenase? Meaning of catechol 2,3-dioxygenase medical term. What does catechol 2,3-dioxygenase mean? ... Looking for online definition of catechol 2,3-dioxygenase in the Medical Dictionary? catechol 2,3-dioxygenase explanation free ... catechol 2,3-dioxygenase. cat·e·chol 2,3-di·ox·y·gen·ase. (katĕ-kol dē-oksē-jenās), An oxidoreductase oxidizing catechol, ... a href=https://medical-dictionary.thefreedictionary.com/catechol+2%2c3-dioxygenase,catechol 2,3-dioxygenase,/a,. *Facebook ...
... the crystal structure of catechol 2,3-dioxygenase (metapyrocatechase) from Ppseudomonas putida mt-2. ... STRUCTURE OF CATECHOL 2,3-DIOXYGENASE (METAPYROCATECHASE) FROM PSEUDOMONAS PUTIDA MT-2. ...
... the crystal structure of catechol 2,3-dioxygenase (metapyrocatechase) from Ppseudomonas putida mt-2. ... Description: CATECHOL 2,3-DIOXYGENASE protein , Length: 307 No structure alignment results are available for 1MPY.A, 1MPY.B, ... STRUCTURE OF CATECHOL 2,3-DIOXYGENASE (METAPYROCATECHASE) FROM PSEUDOMONAS PUTIDA MT-2. ... 3] Andreeva A., Howorth D., Chandonia J.-M., Brenner S.E., Hubbard T.J.P., Chothia C., Murzin A.G. (2008).. Data growth and its ...
Crystal structure determination of Catechol 1,2-Dioxygenase from Rhodococcus opacus 1CP in complex with 3,5-dichlorocatechol ... ion containing enzyme specialized in the aerobic biodegradation of catechols, and its adducts with catechol, 3-methylcatechol, ... Catechol 1,2-dioxygenase from the Gram-positive Rhodococcus opacus 1CP: Quantitative structure/activity relationship and the ... The structural analyses reveal the diverse modes of binding to the active metal iron ion of the tested catechols thus allowing ...
Grouping of phenol hydroxylase and catechol 2,3-dioxygenase genes among phenol- and p-cresol-degrading Pseudomonas species and ... Grouping of phenol hydroxylase and catechol 2,3-dioxygenase genes among phenol- and p-cresol-degrading Pseudomonas species and ... Grouping of phenol hydroxylase and catechol 2,3-dioxygenase genes among phenol- and p-cresol-degrading Pseudomonas species and ... Merimaa, M.; Heinaru, E.; Liivak, M.; Vedler, E.; Heinaru, A. (2006). Grouping of phenol hydroxylase and catechol 2,3- ...
... a family of catechol dioxygenases that cleaves the bond between the phenolic hydroxyl groups of catechol using an Fe3+ cofactor ... catechol with catechol dioxygenases". Arch. Biochem. Biophys. 248 (7): 130-137. doi:10.1016/0003-9861(86)90409-1. PMID 3015028 ... Two families of dioxygenases were discovered by Osamu Hayaishi and Kizo Hashimoto in 1950: catechol 1,2-dioxygenase and ... catechol dioxygenase, pyrocatechase, pyrocatechol 1,2-dioxygenase, CD I, CD II) is an enzyme that catalyzes the oxidative ring ...
The catechol degradation (Ctd) enzymes were identified based on the analysis of the JS42 genome sequence (). CtdE1, catechol 2, ... strain JS42 to 2-nitrotoluene.. Rabinovitch-Deere CA1, Parales RE.. Author information. 1. Department of Microbiology, College ... Fig 2. Wild-type JS42m responses in the qualitative capillary assay. Responses to buffer (negative control) or succinate (10 mM ... Fig 3. Chemotactic response to nitrite and generation of nitrite from 2NT during the qualitative capillary assay performed with ...
IPR017624. Catechol_2-3_dOase. IPR029068. Glyas_Bleomycin-R_OHBP_Dase. IPR004360. Glyas_Fos-R_dOase_dom. IPR037523. VOC. ... Belongs to the extradiol ring-cleavage dioxygenase family.Curated. ,p>UniProtKB Keywords constitute a ,a href="http://www. ... IPR017624. Catechol_2-3_dOase. IPR029068. Glyas_Bleomycin-R_OHBP_Dase. IPR004360. Glyas_Fos-R_dOase_dom. IPR037523. VOC. ... Catechol + O2 = 2-hydroxymuconate-6-semialdehyde.. ,p>This subsection of the Function section provides information relevant ...
The XylE (catechol 2,3-dioxygenase) assays were determined spectrophotometrically at 30°C in a total volume of 3 ml of 100 mM ... Catechol 2,3-dioxygenase assays.For enzymatic assays, overnight cultures were diluted 1:50 in 250 ml of tryptic soy broth ... aureus mutant strains and assayed for the activity of catechol 2,3-dioxygenase, an enzyme which is the gene product of thexylE ... were lysed with lysostaphin and assayed for catechol 2,3-dioxygenase activity as described previously. The average values for ...
Dioxygenases * Catechol 2,3-Dioxygenase Associated data * GENBANK/U03991 * GENBANK/U03992 * GENBANK/U17499 ...
catechol 2,3 dioxygenase [KO:K00446] [EC:1.13.11.2]. Mrub_1083 Glyoxalase/bleomycin resistance protein/dioxygenase [KO:K07104 ... 4-dioxygenase, alpha subunit [KO:K00448] [EC:1.13.11.3]. Mrub_2693 ... 3-hydroxyacyl-CoA dehydrogenase NAD-binding protein [KO:K07516] [EC:1.1.1.35] ... 2-Hydroxycyclohexanecarboxyl coenzyme A dehydrogenase, an enzyme characteristic of the anaerobic benzoate degradation pathway ...
Catechol dioxygenases are metalloprotein enzymes that carry out the oxidative cleavage of catechols. This class of enzymes ... 4-dioxygenase (EC 1.13.11.3). The active site of catechol dioxygenases most frequently contains iron, but manganese-containing ... Catechol dioxygenases belong to the class of oxidoreductases and have several different substrate specificities, including ... An example of the reaction carried out by catechol 1,2-dioxygenase is the formation of cis,cis-muconic acid from catechol, ...
From Catechol to 2-Hydroxy-cis,cis-muconate semialdehyde. Graphic of the reaction.. Medline reference Cerdan P, Wasserfallen A ... Catechol , , O2 catechol , / 2,3-dioxygenase ,/ 1.13.11.2 , Search GenBank, 925 hits on Apr. 03, 2012 Kyoto ,\ ExPASy , \ , H+ ... Search Medline titles for catechol 2,3-dioxygenase. 77 citations on July 26, 2012. ... v 2-Hydroxy-cis,cis-muconate semialdehyde Display a pathway starting from this reaction. EAWAG-BBD Biotransformation rules in ...
... lysed to obtain cell lysate with catechol dioxygenase activity for determination of kinetic parameters of catechol dioxygenase ... Growth with Catechol (LB) The addition of catechol had distinctive effects on the XylE expressing cells growing in LB medium. ... The cleavage of the GFP by TEV protease, which is synthesised on arrival of the input signal, means that Catechol dioxygenase ... Reversion of the reaction profile back to the kinetics of the wild type catechol dioxygenase would mean that we have ...
Catechol dioxygenase activity assays.Catechol dioxygenase (XylE) activity assays were performed as described in reference18. ... which encodes catechol dehydrogenase and converts colorless catechol to an intensely yellow oxidation product (hydroxymuconic ... Within orf261is the Sau3AI site of fusion of SLP1 to the xylEgene of pXE4, indicating that production of catechol dehydrogenase ... Appearance of yellow colonies was determined visually no more than 1 h after spreading of the catechol in the initial screen ...
Molecular Characterization of Aniline Biodegradation by Some Bacterial Isolates having Unexpressed Catechol 2,3-Dioxygenase ... In this study, we fabricated hybrid [email protected][Fe.sub.3][O.sub.4] by the in situ aniline polymerization at the spherical of [Fe.sub.3 ... a colorless, oily, slightly water-soluble liquid, C6H5NH2, used chiefly in the synthesis of dyes and drugs. ... Silver nitrate (AgN[O.sub.3]) and aniline chloride (AnCl) were purchased from Sigma-Aldrich.. Spectroscopic Study of ...
... 87 endo-cleaving rubber dioxygenase. EC 1.13.11.1. Accepted name: catechol 1,2-dioxygenase. Reaction: catechol + O2 ... EC 1.13.11.18 sulfur dioxygenase. EC 1.13.11.19 cysteamine dioxygenase. EC 1.13.11.20 cysteine dioxygenase. EC 1.13.11.21 now ... A novel non-heme iron-containing dioxygenase. Chloridazon-catechol dioxygenase from Phenylobacterium immobilis DSM 1986. Eur. J ... Other name(s): 10R-DOX; (10R)-dioxygenase; 10R-dioxygenase. Systematic name: linoleate:oxygen (10R)-oxidoreductase. Comments: ...
... (meta ring cleavage). When cursor points to a box further details will be displayed in a tooltip window. If ... catechol biosynthesis. EC 1.2.1.10 acetaldehyde dehydrogenase (acetylating). EC 1.2.1.85 2-hydroxymuconate-6-semialdehyde ... EC 1.13.11.2 catechol 2,3-dioxygenase. EC 3.7.1.9 2-hydroxymuconate-6-semialdehyde hydrolase. EC 4.1.1.77 4-oxalocrotonate ... EC 4.1.3.39 4-hydroxy-2-oxovalerate aldolase. EC 4.2.1.80 2-oxopent-4-enoate hydratase. EC 5.3.2.6 2-hydroxymuconate ...
Insights into the binding interaction of substrate with catechol 2,3-dioxygenase from biophysics point of view. ... 3.. Bioinspired red blood cell membrane-encapsulated biomimetic nanoconstructs for synergistic and efficacious chemo- ... Paenibacillus polymyxa biofertilizer application in a tea plantation reduces soil N2O by changing denitrifier communities. ... A novel amino acid site closely associated with the neurovirulence of live, attenuated Japanese encephalitis vaccine (SA14-14-2 ...
To promote crosslinking, other enzymes also may be used, such as catechol 2,3-dioxygenase. FIG. 3 is an illustrative gel (SDS ... with the conversion of tyrosine to dopa occurring by the action of a catechol oxidase (a `tyrosinase` enzyme) present in the ... 2): (Xaa1 -Xaa1 -Xaa1 -Xaa2 -Xaa3 -Xaa2 -Xaa1 -Xaa1 -Xaa1 -Xaa1 -Xaa3 -Xaa2 -Xaa3 -Xaa1)b ... 2): (Xaa1 -Xaa1 -Xaa1 -Xaa2 -Xaa3 -Xaa2 -Xaa1 -Xaa1 -Xaa1 -Xaa1 -Xaa3 -Xaa2 -Xaa3 -Xaa1)b ...
The isofunctional enzymes of catechol 1,2-dioxygenase from species of Acinetobacter, Pseudomonas, Nocardia, Alcaligenes and ... Extradiol Cleavage of 3-Methylcatechol by Catechol 1,2-Dioxygenase from Various Microorganisms. C. T. Hou, R. Patel and M. O. ... The 3-methylcatechol structural motif is rare in natural products. Known examples include calopin and a δ-lactone derivative, O ... 3-Methylcatechol is a chemical compound. The enzyme 1,2-dihydroxy-6-methylcyclohexa-3,5-dienecarboxylate dehydrogenase uses 1,2 ...
Muller R, Schmitt S, Lingens F (1982). "A novel non-heme iron-containing dioxygenase. Chloridazon-catechol dioxygenase from ... Chloridazon-catechol dioxygenase (EC 1.13.11.36) is an enzyme that catalyzes the chemical reaction 5-amino-4-chloro-2-(2,3- ... Muller R, Haug S, Eberspacher J, Lingens F (1977). "[Catechol 2,3-dioxygenase from pyrazon-degrading bacteria (authors transl ... 125 (3): 579-84. doi:10.1111/j.1432-1033.1982.tb06722.x. PMID 6811270. Molecular and Cellular Biology portal. ...
  • 2. Role of oxygen in the metabolic regulation : Besides the well known … More role of oxygen in the energy metabolism, oxygen has been shown to play an important physiological role in metabolic regulation through oxygenase-catalyzed reactions. (nii.ac.jp)
  • For the elucidation of the active site structure of another well characterized oxygenase, catechol 2, 3 - dioxygense, we used o-nitrophenol, a competitive inhibitor, and found it a useful active site probe for this enzyme. (nii.ac.jp)
  • In 2010, the Lethbridge iGEM team confirmed that xylE when introduce to E. coli hydrolyzes catechol to 2-HMS demonstrating and that this critical step can successfully be performed to convert toxic chemicals to metabolic intermediates 7 . (igem.org)
  • Six species of free-living nitrogen fixing bacteria, Azomonas agilis, Azospirillum brasilense, Azospirillum lipoferum, Azotobacter chroococcum, Azotobacter vinelandii , and Beijerinckia mobilis , were surveyed for their ability to grow and fix N 2 using aromatic compounds as sole carbon and energy source. (springer.com)
  • Dorn E, Knackmuss H-J (1978) Chemical structure and biodegradability of halogenated aromatic compounds: two catechol 1,2-dioxygenases from a 3-chlorobenzoate grown pseudomonad. (springer.com)
  • Studies of Porphyrin Metabolism, 3. (unt.edu)
  • Metabolism of 4-amino-3-hydroxybenzoic acid by Bordetella sp. (wikipedia.org)
  • It is noteworthy that in nature 3-HP serves in central metabolism either as an intermediate of C3/C4 assimilation cycles or as an input carbon source in some bacteria and archaea. (biomedcentral.com)
  • A quantitative relationship (R=0.966) between the ln k(cat) and the calculated electronic parameter E(HOMO) was obtained for catechol, 3-methylcatechol, 4-methylcatechol, pyrogallol, 3-chlorocatechol, 4-chlorocatechol. (rcsb.org)
  • Preferentially converts 3-chlorocatechol and 3,5-dichlorocatechol as opposed to other chlorinated catechols. (rcsb.org)
  • In a number of early studies ( 15 , 17 , 22 ) pWW0 was found to carry insertions of 3 to 3.5 kb in various locations, usually within the catabolic regions, where their presence has been associated with a change in the catabolic phenotype. (asm.org)
  • Jones DCN, Cooper RA (1990) Catabolism of 3-hydroxybenzoate by the gentisate pathway in Klebsiella pneumoniae M5a1. (springer.com)
  • It has been much studied, mainly because of the four xyl regulons which encode the inducible catabolism of toluene and some substituted toluenes to central metabolites ( 2 , 33 ). (asm.org)
  • Although Escherichia coli or Saccharomyces cerevisiae are the primary industrial microbes for the production of 3-HP, alternative engineered hosts have the potential to generate 3-HP from other carbon feedstocks. (biomedcentral.com)
  • In vitro enzymatic assay and β-alanine pathway dependent 13 C-labeling further demonstrated that a reductive route sequentially converted 3-HP-CoA to acrylyl-CoA and propionyl-CoA, with the latter being reassimilated into the ethylmalonyl-CoA pathway. (biomedcentral.com)
  • The impact of five crucial variables on enzyme production, including glucose and nitrogen concentrations and three distinct putative inducers, namely, copper sulphate, 2,5-xylidine and olive-mill wastewater was thoroughly investigated. (chemweb.com)
  • in the degradative pathways of 2-aminobenzenesulphonic, benzenesulphonic and 4-toluenesulphonic acids in Alcaligenes sp. (wikipedia.org)
  • To address the decontamination of (1) toxic organic compounds such as naphthene and catechol found in the tailings ponds, natural pathways that can break down the toxic chemicals can be utilized. (igem.org)
  • In recent years, 2-D electrophoresis has been widely accepted as a standard procedure to separate complex protein mixtures in proteome studies (Proteomics). (wikipedia.org)
  • With the medium thus optimised, further experiments were performed in a 3-L stirred-tank reactor (STR) leading to a laccase activity of 2200 IU/L on day 9 with a productivity of 10.2 IU/(L h) and a specific activity of 26.7 IU/mg protein. (chemweb.com)
  • The sequences of the α and β subunits of the dioxin dioxygenase exhibit only weak similarity to other three component dioxygenases, but some motifs such as the Fe(II) binding site and the [2Fe-2S] cluster ligands are conserved. (asm.org)
  • Extradiol members utilize ferrous iron as the active redox state, and this center is commonly coordinated octahedrally through a 2-His-1-Glu motif with labile water ligands occupying empty positions. (wikipedia.org)
  • During their existence as extrachromosomal replicons, most integrating plasmids share with other types of Streptomyces plasmids the ability to undergo conjugal transfer and inhibit transiently the growth of plasmid recipients, yielding zones of slowed growth called pocks ( 3 ). (asm.org)
  • SLP1, which was the first reintegrating plasmid discovered in Streptomyces ( 4 ), exists normally as a 17.2-kb plasmidogenic sequence (SLP1 int ) in the chromosome of Streptomyces coelicolor A3(2). (asm.org)
  • Plasmid, 70 (3), 393−405.10.1016/j.plasmid.2013.09.003. (etis.ee)
  • The result is a smaller, but still conjugative, 78-kb plasmid, pWW0-8, which is phenotypically cryptic ( 3 , 7 ) and carries only a single copy of IS 1246 . (asm.org)
  • Two key metabolites of the meta-cleavage pathway, 5-formyl-2-hydroxy-6,6,8,8-tetramethylnona-2,4-dienoic acid and 6,6,8,8-tetramethyl-2-oxonon-4-enoic acid, were detected by LC-ESI-Q-TOF. (ncu.edu.tw)
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