A group of 1,2-benzenediols that contain the general formula R-C6H5O2.
Catalyzes the oxidation of catechol to 2-hydroxymuconate semialdehyde in the carbazole and BENZOATE degradation via HYDROXYLATION pathways. It also catalyzes the conversion of 3-methylcatechol to cis, cis-2-hydroxy-6-oxohept-2,4-dienoate in the TOLUENE and XYLENE degradation pathway. This enzyme was formerly characterized as EC
Non-heme iron-containing enzymes that incorporate two atoms of OXYGEN into the substrate. They are important in biosynthesis of FLAVONOIDS; GIBBERELLINS; and HYOSCYAMINE; and for degradation of AROMATIC HYDROCARBONS.
An enzyme that catalyzes the oxidation of catechol to muconic acid with the use of Fe3+ as a cofactor. This enzyme was formerly characterized as EC and EC
Oxidases that specifically introduce DIOXYGEN-derived oxygen atoms into a variety of organic molecules.
2- or 4-Hydroxyestrogens. Substances that are physiologically active in mammals, especially in the control of gonadotropin secretion. Physiological activity can be ascribed to either an estrogenic action or interaction with the catecholaminergic system.
An enzyme that catalyzes the conversion of 4-hydroxyphenylpyruvate plus oxygen to homogentisic acid and carbon dioxide. EC
An enzyme that catalyzes the oxidation of protocatechuate to 3-carboxy-cis-cis-muconate in the presence of molecular oxygen. It contains ferric ion. EC
An enzyme that catalyzes the conversion of L-CYSTEINE to 3-sulfinoalanine (3-sulfino-L-alanine) in the CYSTEINE metabolism and TAURINE and hypotaurine metabolic pathways.
A dioxygenase with specificity for the oxidation of the indoleamine ring of TRYPTOPHAN. It is a LIVER-specific enzyme that is the first and rate limiting enzyme in the kynurenine pathway of TRYPTOPHAN catabolism.
Enzyme that catalyzes the movement of a methyl group from S-adenosylmethionone to a catechol or a catecholamine.
A mononuclear Fe(II)-dependent oxygenase, this enzyme catalyzes the conversion of homogentisate to 4-maleylacetoacetate, the third step in the pathway for the catabolism of TYROSINE. Deficiency in the enzyme causes ALKAPTONURIA, an autosomal recessive disorder, characterized by homogentisic aciduria, OCHRONOSIS and ARTHRITIS. This enzyme was formerly characterized as EC and EC
An enzyme that catalyzes the conversion of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde. It was formerly characterized as EC
A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.
Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.
A species of gram-negative, aerobic bacteria isolated from soil and water as well as clinical specimens. Occasionally it is an opportunistic pathogen.
Benzoate derivatives substituted by one or more hydroxy groups in any position on the benzene ring.
A bacterial genus of the order ACTINOMYCETALES.
An antiseptic and disinfectant aromatic alcohol.
Mold and yeast inhibitor. Used as a fungistatic agent for foods, especially cheeses.
An enzyme of the oxidoreductase class that catalyzes the reaction between catechol and oxygen to yield benzoquinone and water. It is a complex of copper-containing proteins that acts also on a variety of substituted catechols. EC
An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals.
Benzoic acid or benzoic acid esters substituted with one or more chlorine atoms.
A widely used industrial solvent.
Derivatives of BENZOIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxybenzene structure.
Widely distributed enzymes that carry out oxidation-reduction reactions in which one atom of the oxygen molecule is incorporated into the organic substrate; the other oxygen atom is reduced and combined with hydrogen ions to form water. They are also known as monooxygenases or hydroxylases. These reactions require two substrates as reductants for each of the two oxygen atoms. There are different classes of monooxygenases depending on the type of hydrogen-providing cosubstrate (COENZYMES) required in the mixed-function oxidation.
A genus of gram-negative, aerobic, rod-shaped bacteria characterized by an outer membrane that contains glycosphingolipids but lacks lipopolysaccharide. They have the ability to degrade a broad range of substituted aromatic compounds.
A genus of gram-negative, aerobic, rod-shaped bacteria. Organisms in this genus had originally been classified as members of the PSEUDOMONAS genus but overwhelming biochemical and chemical findings indicated the need to separate them from other Pseudomonas species, and hence, this new genus was created.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A fungistatic compound that is widely used as a food preservative. It is conjugated to GLYCINE in the liver and excreted as hippuric acid.
Salts and esters of gentisic acid.
A genus of gram-negative, aerobic, motile bacteria that occur in water and soil. Some are common inhabitants of the intestinal tract of vertebrates. These bacteria occasionally cause opportunistic infections in humans.
Toxic, volatile, flammable liquid hydrocarbon byproduct of coal distillation. It is used as an industrial solvent in paints, varnishes, lacquer thinners, gasoline, etc. Benzene causes central nervous system damage acutely and bone marrow damage chronically and is carcinogenic. It was formerly used as parasiticide.
Derivatives of adipic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a 1,6-carboxy terminated aliphatic structure.
An estrogenic steroid produced by HORSES. It has a total of five double bonds in the A- and B-ring. High concentration of equilenin is found in the URINE of pregnant mares.
A genus of gram-negative, straight or slightly curved rods which are motile by polar flagella and which accumulate poly-beta-hydroxybutyrate within the cells.
The functional hereditary units of BACTERIA.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.
A trihydroxybenzene or dihydroxy phenol that can be prepared by heating GALLIC ACID.
A gram-positive organism found in dairy products, fresh and salt water, marine organisms, insects, and decaying organic matter.
Organic compounds containing carbon and hydrogen in the form of an unsaturated, usually hexagonal ring structure. The compounds can be single ring, or double, triple, or multiple fused rings.
Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.
A genus in the family BURKHOLDERIACEAE, comprised of many species. They are associated with a variety of infections including MENINGITIS; PERITONITIS; and URINARY TRACT INFECTIONS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A genus of coccoid bacteria in the family PLANOCOCCACEAE. They are widely distributed in various habitats including sea water, freshwater ponds, cyanobacterial mats, and in marine animals.
A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.
Placing of a hydroxyl group on a compound in a position where one did not exist before. (Stedman, 26th ed)
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Methyl, propyl, butyl, and ethyl esters of p-hydroxybenzoic acid. They have been approved by the FDA as antimicrobial agents for foods and pharmaceuticals. (From Hawley's Condensed Chemical Dictionary, 11th ed, p872)
Industrial products consisting of a mixture of chlorinated biphenyl congeners and isomers. These compounds are highly lipophilic and tend to accumulate in fat stores of animals. Many of these compounds are considered toxic and potential environmental pollutants.
A group of gram-negative bacteria consisting of rod- and coccus-shaped cells. They are both aerobic (able to grow under an air atmosphere) and microaerophilic (grow better in low concentrations of oxygen) under nitrogen-fixing conditions but, when supplied with a source of fixed nitrogen, they grow as aerobes.
Iron-containing proteins that transfer electrons, usually at a low potential, to flavoproteins; the iron is not present as in heme. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
The rate dynamics in chemical or physical systems.
A family of gram negative, aerobic, non-sporeforming, rod-shaped bacteria.
An oxidation product of tryptophan metabolism. It may be a free radical scavenger and a carcinogen.
An enzyme that catalyzes the HYDROXYLATION of gamma-butyrobetaine to L-CARNITINE. It is the last enzyme in the biosynthetic pathway of L-CARNITINE and is dependent on alpha-ketoglutarate; IRON; ASCORBIC ACID; and OXYGEN.
A genus of asporogenous bacteria isolated from soil that displays a distinctive rod-coccus growth cycle.
An inborn error of amino acid metabolism resulting from a defect in the enzyme HOMOGENTISATE 1,2-DIOXYGENASE, an enzyme involved in the breakdown of PHENYLALANINE and TYROSINE. It is characterized by accumulation of HOMOGENTISIC ACID in the urine, OCHRONOSIS in various tissues, and ARTHRITIS.
A family of isomeric, colorless aromatic hydrocarbon liquids, that contain the general formula C6H4(CH3)2. They are produced by the destructive distillation of coal or by the catalytic reforming of petroleum naphthenic fractions. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
A family of compounds containing an oxo group with the general structure of 1,5-pentanedioic acid. (From Lehninger, Principles of Biochemistry, 1982, p442)
The salts or esters of salicylic acids, or salicylate esters of an organic acid. Some of these have analgesic, antipyretic, and anti-inflammatory activities by inhibiting prostaglandin synthesis.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Enzymes of the isomerase class that catalyze reactions in which a group can be regarded as eliminated from one part of a molecule, leaving a double bond, while remaining covalently attached to the molecule. (From Enzyme Nomenclature, 1992) EC 5.5.
Inorganic or organic compounds that contain divalent iron.
An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.
Benzoic acids, salts, or esters that contain an amino group attached to carbon number 2 or 6 of the benzene ring structure.
A flavoring agent. It is the intermediate product in the two-step bioconversion of ferulic acid to vanillin. (J Biotechnol 1996;50(2-3):107-13).
The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.
Proteins found in any species of bacterium.
Aminobenzenesulfonic acids. Organic acids that are used in the manufacture of dyes and organic chemicals and as reagents.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A group of proteins possessing only the iron-sulfur complex as the prosthetic group. These proteins participate in all major pathways of electron transport: photosynthesis, respiration, hydroxylation and bacterial hydrogen and nitrogen fixation.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Two-ring crystalline hydrocarbons isolated from coal tar. They are used as intermediates in chemical synthesis, as insect repellents, fungicides, lubricants, preservatives, and, formerly, as topical antiseptics.
Estrone derivatives substituted with one or more hydroxyl groups in any position. They are important metabolites of estrone and other estrogens.
A metabolite of tryptophan with a possible role in neurodegenerative disorders. Elevated CSF levels of quinolinic acid are correlated with the severity of neuropsychological deficits in patients who have AIDS.
The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
A species of gram-negative bacteria in the genus PSEUDOMONAS, containing multiple genomovars. It is distinguishable from other pseudomonad species by its ability to use MALTOSE and STARCH as sole carbon and energy sources. It can degrade ENVIRONMENTAL POLLUTANTS and has been used as a model organism to study denitrification.
A species of gram-negative rod-shaped bacteria found ubiquitously and formerly called Comamonas acidovorans and Pseudomonas acidovorans. It is the type species of the genus DELFTIA.
A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.
Phenols substituted with one or more chlorine atoms in any position.
A species of gram-negative, aerobic bacteria found in soil and water. Although considered to be normally nonpathogenic, this bacterium is a causative agent of nosocomial infections, particularly in debilitated individuals.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
A deaminated metabolite of LEVODOPA.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
A species of BURKHOLDERIA considered to be an opportunistic human pathogen. It has been associated with various types of infections of nosocomial origin.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
Any member of the class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting molecules, e.g., ESTERASES, glycosidases (GLYCOSIDE HYDROLASES), lipases, NUCLEOTIDASES, peptidases (PEPTIDE HYDROLASES), and phosphatases (PHOSPHORIC MONOESTER HYDROLASES). EC 3.
A monooxygenase that catalyzes the conversion of BETA-CAROTENE into two molecules of RETINAL. It was formerly characterized as EC and EC
Stable oxygen atoms that have the same atomic number as the element oxygen, but differ in atomic weight. O-17 and 18 are stable oxygen isotopes.
Compounds which restore enzymatic activity by removing an inhibitory group bound to the reactive site of the enzyme.
A group of disorders which have in common elevations of tyrosine in the blood and urine secondary to an enzyme deficiency. Type I tyrosinemia features episodic weakness, self-mutilation, hepatic necrosis, renal tubular injury, and seizures and is caused by a deficiency of the enzyme fumarylacetoacetase. Type II tyrosinemia features INTELLECTUAL DISABILITY, painful corneal ulcers, and keratoses of the palms and plantar surfaces and is caused by a deficiency of the enzyme TYROSINE TRANSAMINASE. Type III tyrosinemia features INTELLECTUAL DISABILITY and is caused by a deficiency of the enzyme 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE. (Menkes, Textbook of Child Neurology, 5th ed, pp42-3)
Naphthalene derivatives carrying one or more hydroxyl (-OH) groups at any ring position. They are often used in dyes and pigments, as antioxidants for rubber, fats, and oils, as insecticides, in pharmaceuticals, and in numerous other applications.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Proteins prepared by recombinant DNA technology.
The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.
An herbicide with irritant effects on the eye and the gastrointestinal system.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.
A highly volatile inhalation anesthetic used mainly in short surgical procedures where light anesthesia with good analgesia is required. It is also used as an industrial solvent. Prolonged exposure to high concentrations of the vapor can lead to cardiotoxicity and neurological impairment.
A species of gram-negative bacteria in the genus PSEUDOMONAS. All strains can utilize FRUCTOSE for energy. It is occasionally isolated from humans and some strains are pathogenic to WATERMELON.
Low-molecular-weight compounds produced by microorganisms that aid in the transport and sequestration of ferric iron. (The Encyclopedia of Molecular Biology, 1994)
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Hydrocarbon rings which contain two ketone moieties in any position. They can be substituted in any position except at the ketone groups.
A large group of aerobic bacteria which show up as pink (negative) when treated by the gram-staining method. This is because the cell walls of gram-negative bacteria are low in peptidoglycan and thus have low affinity for violet stain and high affinity for the pink dye safranine.
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
An enzyme of the oxidoreductase class that catalyzes the reaction between L-tyrosine, L-dopa, and oxygen to yield L-dopa, dopaquinone, and water. It is a copper protein that acts also on catechols, catalyzing some of the same reactions as CATECHOL OXIDASE. EC
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
A class of enzymes that catalyze geometric or structural changes within a molecule to form a single product. The reactions do not involve a net change in the concentrations of compounds other than the substrate and the product.(from Dorland, 28th ed) EC 5.
A colorless or slightly yellow crystalline compound obtained from nutgalls. It is used in photography, pharmaceuticals, and as an analytical reagent.
A colorimetric reagent for iron, manganese, titanium, molybdenum, and complexes of zirconium. (From Merck Index, 11th ed)
A species of gram-negative bacteria in the genus PSEUDOMONAS, which is found in SOIL and WATER.
Substances which pollute the soil. Use for soil pollutants in general or for which there is no specific heading.
Compounds that contain a BENZENE ring fused to a furan ring.
An NADPH-dependent flavin monooxygenase that plays a key role in the catabolism of TRYPTOPHAN by catalyzing the HYDROXYLATION of KYNURENINE to 3-hydroxykynurenine. It was formerly characterized as EC and EC
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
Proteins, usually acting in oxidation-reduction reactions, containing iron but no porphyrin groups. (Lehninger, Principles of Biochemistry, 1993, pG-10)
Pigment obtained by the oxidation of epinephrine.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.
The relationships of groups of organisms as reflected by their genetic makeup.
Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A methylated metabolite of norepinephrine that is excreted in the urine and found in certain tissues. It is a marker for tumors.
Enzymes that catalyze the addition of a carboxyl group to a compound (carboxylases) or the removal of a carboxyl group from a compound (decarboxylases). EC 4.1.1.
A family of gram-negative aerobic bacteria in the class BETA PROTEOBACTERIA, encompassing the acidovorans rRNA complex. Some species are pathogenic for PLANTS.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A conditionally essential nutrient, important during mammalian development. It is present in milk but is isolated mostly from ox bile and strongly conjugates bile acids.
Junca, H.; Plumeier, I.; Hecht, H. J. R.; Pieper, D. H. (December 2004). "Difference in kinetic behaviour of catechol 2,3- ... dioxygenase variants from a polluted environment". Microbiology. 150 (12): 4181-4187. doi:10.1099/mic.0.27451-0. PMID 15583170 ... 227 (1-2): 21-30. doi:10.1016/j.mce.2004.08.001. PMID 15501581. Görg, A.; Weiss, W.; Dunn, M. J. (December 2004). "Current two- ... 3.0.CO;2-C. PMID 11678039. Archived from the original (PDF) on 2010-04-01. "Archived copy". Archived from the original on 2011- ...
The isofunctional enzymes of catechol 1,2-dioxygenase from species of Acinetobacter, Pseudomonas, Nocardia, Alcaligenes and ... Extradiol Cleavage of 3-Methylcatechol by Catechol 1,2-Dioxygenase from Various Microorganisms. C. T. Hou, R. Patel and M. O. ... The 3-methylcatechol structural motif is rare in natural products. Known examples include calopin and a δ-lactone derivative, O ... 3-Methylcatechol is a chemical compound. The enzyme 1,2-dihydroxy-6-methylcyclohexa-3,5-dienecarboxylate dehydrogenase uses 1,2 ...
Sala-Trepat JM, Evans WC (1971). "The meta cleavage of catechol by Azotobacter species 4-Oxalocrotonate pathway". Eur. J. ... 2-Hydroxymuconate semialdehyde is formed from catechol by the enzyme catechol 2,3-dioxygenase during the degradation of ... It is hydrolysed into formate and 2-oxopent-4-enoate by 2-hydroxymuconate-semialdehyde hydrolase. ... 20 (3): 400-13. doi:10.1111/j.1432-1033.1971.tb01406.x. PMID 4325686.. ...
... may refer to: catechol dioxygenase Catechol 1,2-dioxygenase Catechol 2,3-dioxygenase This disambiguation page lists ...
4-dicarboxylate dehydrogenase into a catechol intermediate. The catechol ring is then cleaved by PCA 3,4-dioxygenase before the ... Ideonella sakaiensis PET surface and use a secreted PET hydrolase, or PETase, to degrade the PET into mono(2-hydroxyethyl) ... Once in the cell, the aromatic terephthalic acid molecule is oxidized by terephthalic acid-1,2-dioxygenase and 1,2-dihydroxy-3, ... Pearce, B. A.; Heydeman, M. T. (1 May 1980). "Metabolism of Di(ethylene glycol) [2-(2'-Hydroxyethoxy)ethanol] and Other Short ...
... catechol 2,3-dioxygenase), and is an intermediate in the bacterial degradation of several aromatic compounds. Inoue J, Shaw JP ... 2-Hydroxymuconate-6-semialdehyde dehydrogenase (EC, xylG [gene], praB [gene] ) is an enzyme with systematic name (2E, ... 2-hydroxymuconate-6-semialdehyde+dehydrogenase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology ... Kasai D, Fujinami T, Abe T, Mase K, Katayama Y, Fukuda M, Masai E (November 2009). "Uncovering the protocatechuate 2,3-cleavage ...
4-dioxygenase uses 3,4-dihydroxybenzoate and O2 to produce 3-carboxy-cis,cis-muconate. Ethyl protocatechuate Dawson, R. M. C.; ... 4-dihydroxybenzoate to produce catechol and CO2. The enzyme protocatechuate 3, ... 4-dioxygenase vanillate monooxygenase 3,4-dihydroxyphthalate decarboxylase 4,5-dihydroxyphthalate decarboxylase Degradation ... 14 (3): 276-283. Hassan, S. T. S.; Švajdlenka, E.; Berchová-Bímová, K. (April 2017). "Hibiscus sabdariffa L. and its bioactive ...
... catechol 2,3-dioxygenase, 3,4-dihydroxyphenylacetate 2,3-dioxygenase and 4-hydroxyphenylpyruvate dioxygenase all resemble ... 314 ( Pt 2) (2): 463-7. doi:10.1042/bj3140463. PMC 1217073. PMID 8670058. Saint-Jean AP, Phillips KR, Creighton DJ, Stone MJ ( ... 221 (2): 297-315. doi:10.1007/s00213-011-2574-z. PMC 3337404. PMID 22113448. Thornalley PJ (May 2006). "Unease on the role of ... 190 (2): 685-96. doi:10.1016/S0021-9258(18)56017-8. PMID 14841219. Allen RE, Lo TW, Thornalley PJ (April 1993). "A simplified ...
"Characterization of an O2 Adduct of an Active Cobalt-Substituted Extradiol-Cleaving Catechol Dioxygenase". Journal of the ... "A Hyperactive Cobalt-Substituted Extradiol-Cleaving Catechol Dioxygenase". Journal of Biological Inorganic Chemistry. 16 (2): ... which mediates the electron transfer between catechol substrates and O2 to form a [M(II)(semiquinone)superoxo] intermediate. ... 134 (2): 796-799. doi:10.1021/ja2095365. ISSN 0002-7863. PMC 3262093. PMID 22175783. England, J.; Martinho, M.; Farquhar, E. R ...
... dependent dioxygenases. The catechol dioxygenases, some of the most well-studied dioxygenase enzymes, use dioxygen to cleave a ... Two important groups of mononuclear, non-heme iron dioxygenases are catechol dioxygenases and 2-oxoglutarate (2OG)- ... Catechol dioxygenases are further classified as being "extradiol" or "intradiol," and this distinction is based on mechanistic ... In this respect, these enzymes are reminiscent of the intradiol catechol dioxygenases whereby the metal centers activate the ...
Catechol dioxygenases are metalloprotein enzymes that carry out the oxidative cleavage of catechols. This class of enzymes ... 4-dioxygenase (EC The active site of catechol dioxygenases most frequently contains iron, but manganese-containing ... Catechol dioxygenases belong to the class of oxidoreductases and have several different substrate specificities, including ... An example of the reaction carried out by catechol 1,2-dioxygenase is the formation of cis,cis-muconic acid from catechol, ...
Muller R, Schmitt S, Lingens F (1982). "A novel non-heme iron-containing dioxygenase. Chloridazon-catechol dioxygenase from ... Chloridazon-catechol dioxygenase (EC is an enzyme that catalyzes the chemical reaction 5-amino-4-chloro-2-(2,3- ... Muller R, Haug S, Eberspacher J, Lingens F (1977). "[Catechol 2,3-dioxygenase from pyrazon-degrading bacteria (author's transl ... The systematic name of this enzyme class is 5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone 1,2-oxidoreductase ( ...
... a family of catechol dioxygenases that cleaves the bond between the phenolic hydroxyl groups of catechol using an Fe3+ cofactor ... catechol with catechol dioxygenases". Archives of Biochemistry and Biophysics. 248 (1): 130-7. doi:10.1016/0003-9861(86)90409-1 ... Two families of dioxygenases were discovered by Osamu Hayaishi and Kizo Hashimoto in 1950: catechol 1,2-dioxygenase and ... catechol dioxygenase, pyrocatechase, pyrocatechol 1,2-dioxygenase, CD I, CD II) is an enzyme that catalyzes the oxidative ring ...
Catechol+2,3-dioxygenase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal. ... Catechol 2,3-dioxygenase (EC, 2,3-pyrocatechase, catechol 2,3-oxygenase, catechol oxygenase, metapyrocatechase, ... Kojima Y, Itada N, Hayaishi O (August 1961). "Metapyrocatachase: a new catechol-cleaving enzyme". The Journal of Biological ... is an enzyme with systematic name catechol:oxygen 2,3-oxidoreductase (decyclizing). This enzyme catalyses the following ...
Kobayashi S, Hayaishi O (1970). "Anthranilic acid conversion to catechol (Pseudomonas)". Methods Enzymol. 17A: 505-510. doi: ... catechol + CO2 + NAD(P)+ + NH3 The 5 substrates of this enzyme are anthranilate, NADH, NADPH, H+, and O2, whereas its 5 ... "Enzymatic formation of catechol from anthranilic acid". J. Biol. Chem. 239: 2204-2211. PMID 14209949. Biology portal v t e. ... products are catechol, CO2, NAD+, NADP+, and NH3. This enzyme belongs to the family of oxidoreductases, specifically those ...
Junca, H.; Plumeier, I.; Hecht, H. J. R.; Pieper, D. H. (December 2004). "Difference in kinetic behaviour of catechol 2,3- ... dioxygenase variants from a polluted environment". Microbiology. 150 (12): 4181-4187. doi:10.1099/mic.0.27451-0. PMID 15583170. ... 2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] and a crucial step in gene expression profiling. ... 2 (3): 295-306. doi:10.1586/14789450.2.3.295. PMID 16000077.. *^ Piette, André; Derouaux, Adeline; Gerkens, Pascal; Noens, Elke ...
... dioxygenases MeSH D08.811.682.690.416.277 - catechol 1,2-dioxygenase MeSH D08.811.682.690.416.305 - catechol 2,3-dioxygenase ... 4-dioxygenase MeSH D08.811.682.690.416.330 - 4-hydroxyphenylpyruvate dioxygenase MeSH D08.811.682.690.416.333 - indoleamine- ... 4-dioxygenase MeSH D08.811.682.690.416.722 - tryptophan oxygenase MeSH D08.811.682.690.562 - inositol oxygenase MeSH D08.811. ... catechol oxidase MeSH D08.811.682.690.708.125.500 - monophenol monooxygenase MeSH D08.811.682.690.708.170 - cytochrome p-450 ...
I Enzymatic formation of catechol from benzene". Biochemistry. 7 (7): 2653-62. doi:10.1021/bi00847a031. PMID 4298226. Biology ... and benzene dioxygenase. This enzyme participates in naphthalene and anthracene degradation. It has 4 cofactors: FAD, Iron, ... In enzymology, a benzene 1,2-dioxygenase (EC is an enzyme that catalyzes the chemical reaction benzene + NADH + H+ ... The systematic name of this enzyme class is benzene,NADH:oxygen oxidoreductase (1,2-hydroxylating). Other names in common use ...
other dioxygenase: Catechol dioxygenase. *Homogentisate 1,2-dioxygenase. *Cysteine dioxygenase. *4-Hydroxyphenylpyruvate ... dioxygenase at the US National Library of Medicine Medical Subject Headings (MeSH) ... naphthalene-1,2-diol + O2 ⇌. {\displaystyle \rightleftharpoons }. 2-hydroxy-2H-chromene-2-carboxylate. This enzyme is involved ... 1,2-dihydroxynaphthalene dioxygenase (EC, 1,2-DHN dioxygenase, DHNDO, 1,2-dihydroxynaphthalene oxygenase, 1,2- ...
"The genes coding for the conversion of carbazole to catechol are flanked by IS6100 elements in Sphingomonas sp. strain XLDN2-5 ... 9a-dioxygenase, a three-component dioxygenase system of Pseudomonas resinovorans strain CA10". Applied and Environmental ... Carbazole 1,9a-dioxygenase (EC, CARDO) is an enzyme with systematic name 9H-carbazole,NAD(P)H:oxygen oxidoreductase ... Carbazole+1,9a-dioxygenase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal. ...
Iron-containing dioxygenase enzymes catalyze the cleavage of catechol. Catechol is produced by a reversible two-electron, two- ... catechol was listed as pyrocatechol. In 1879, the Journal of the Chemical Society recommended that catechol be called "catechol ... Catechol occurs as feathery white crystals that are very rapidly soluble in water. Catechol was first isolated in 1839 by Edgar ... Catechols produce quinones with the addition of ceric ammonium nitrate (CAN). Catechol is the conjugate acid of a chelating ...
Catechol dioxygenases. Department of Chemistry, Michigan State University, East Lansing, MI 488241322, U.S.A. ... Diversity of catechol 2,3-dioxygenase genes of bacteria responding to dissolved organic matter derived from different sources ... PCR isolation of catechol 2,3-dioxygenase gene fragments from environmental samples and their assembly into functional genes. ... The Catechol 1,2 Dioxygenase System of Acinetobacter radioresistens: Isoenzymes, Inductors and Gene localisation. Biol. Chem., ...
EC 2.1.1 Catechol-O-methyl transferase EC DNA methyltransferase EC, EC, EC Histone ... 4-hydroxyphenylpyruvate dioxygenase (EC Category:EC 1.13.12 (With incorporation of one atom of oxygen (internal ... Catechol oxidase EC Laccase EC Category:EC 1.10.99 (with other acceptors) Category:EC 1.11.1 (peroxidases) ... EC 1.14.12 Nitric oxide dioxygenase Category:EC 1.14.13 Nitric oxide synthase EC Category:EC 1.14.14 Aromatase EC ...
The developing chemistry is similar to that of catechol or pyrogallol. It is also used as a matrix in MALDI mass spectrometry ... 4-dioxygenase is an enzyme that uses caffeic acid and oxygen to produce 3-(2-carboxyethenyl)-cis,cis-muconate. 3-O- ... 4-methoxyphenol and catechol at low doses, either alone or in combination, and modulation of their effects in a rat medium-term ... 35 (2): 499-505. doi:10.1016/S0031-9422(00)94790-3. Xu, Peng; Uyama, Hiroshi; Whitten, James E.; Kobayashi, Shiro; Kaplan, ...
Heating catechin past its point of decomposition releases pyrocatechol (also called catechol), which explains the common origin ... leucoanthocyanidin dioxygenase), E11, anthocyanidin reductase. HSCoA, Coenzyme A. L-Tyr, L-tyrosine, L-Phe, L-phenylalanine. ... Flavan-3-ols (sometimes referred to as flavanols) are derivatives of flavans that possess a 2-phenyl-3,4-dihydro-2H-chromen-3- ... Only flavan-3-ols with an intact (epi)catechin moiety can be metabolised 5-(3′/4′-dihydroxyphenyl)-γ-valerolactones. The ...
O-rhamnoside 7-O-methyltransferase Catechol-O-methyl transferase 5-desoxy-malvidin Capensinidin Europinidin Hirsutidin Malvidin ... and evidence that partially methylated flavanones are substrates of four different flavonoid dioxygenases". Phytochemistry. 65 ... 119 (2): 155-62. doi:10.1016/j.jbiotec.2005.04.004. PMID 15961179. Schroder, G; Wehinger, E; Lukacin, R; Wellmann, F; Seefelder ... Those positions can be ortho, meta, para and there can be a special 3-O-methyltransferase for the 3-OH position. Calamondin ...
... related with the catechol and resorcinol groups and the oxidation is pH-dependent. The oxidation of the catechol 3',4'- ... 4-dioxygenase and hydroxyquinol 1,2-dioxygenase to form β-carboxy cis, cis-muconic acid and maleyl acetate. Among fungi, ... sulfation and methylation of the catechol group by catechol-O-methyl transferase, with only small amounts detected in plasma. ... The A ring is similar to a resorcinol moiety while the B ring is similar to a catechol moiety. There are two chiral centers on ...
other dioxygenase: Catechol dioxygenase. *Homogentisate 1,2-dioxygenase. *Cysteine dioxygenase. *4-Hydroxyphenylpyruvate ... ALOX5's dioxygenase activity adds a hydroperoxyl (i.e. HO2) residue to arachidonic acid (i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic ... dioxygenase activity. • metal ion binding. • protein binding. • oxidoreductase activity. • oxidoreductase activity, acting on ... 39 (1): 3-22. doi:10.1002/cbin.10345. PMID 25052386.. *^ a b Barden AE, Mas E, Mori TA (2016). "n-3 Fatty acid supplementation ...
Next, the diol is newly reduced by NADH to catechol. The catechol is then metabolized to acetyl CoA and succinyl CoA, used by ... In bacteria, dioxygenase enzyme can add an oxygen to the ring, and the unstable product is immediately reduced (by NADH) to a ... and hydroquinone to both benzenetriol and catechol. Hydroquinone, benzenetriol and catechol are converted to polyphenols. In ... catechol, hydroquinone and 1,2,4-trihydroxybenzene. Most of these metabolites have some value as biomarkers of human exposure, ...
other dioxygenase: Catechol dioxygenase. *Homogentisate 1,2-dioxygenase. *Cysteine dioxygenase. *4-Hydroxyphenylpyruvate ... Dioxygenases, or oxygen transferases, incorporate both atoms of molecular oxygen (O2) into the product(s) of the reaction.[8] ... Bugg TDH (2003). "Dioxygenase enzymes: catalytic mechanisms and chemical models". Tetrahedron. 59 (36): 7075-7101. doi:10.1016/ ... An oxygenase is any enzyme that oxidizes a substrate by transferring the oxygen from molecular oxygen O2 (as in air) to it. The ...
The meta-hydroxyl group of catechol is methylated by catechol-O-methyltransferase. Four of the five hydroxyl groups of ... 2 (5): 235-44. doi:10.1039/c1fo10025d. PMC 4122511. PMID 21779561.. *^ a b Day AJ, Rothwell JA, Morgan R (2004). " ... 60 (3): 595-602. PMID 11502892.. *^ van der Woude H, Ter Veld MG, Jacobs N, van der Saag PT, Murk AJ, Rietjens IM (2005). "The ... 6 (3): 173-93. PMID 17724002.. *^ Maggiolini M, Bonofiglio D, Marsico S, Panno ML, Cenni B, Picard D, Andò S (2001). "Estrogen ...
Oxidoreductases: dioxygenases, including steroid hydroxylases (EC 1.14). 1.14.11: 2-oxoglutarate. *Prolyl hydroxylase ... 16 (3): 285-309. doi:10.1007/BF02741387. PMID 9626667.. *^ a b Teigen K, McKinney JA, Haavik J, Martínez A (2007). "Selectivity ... 10 (6 Pt 2): 774-81. doi:10.1159/000073965. PMID 14631117.. *^ a b Halskau Ø, Ying M, Baumann A, Kleppe R, Rodriguez-Larrea D, ... 116 (3): 1133-1134. doi:10.1021/ja00082a046.. *^ Haavik J, Flatmark T (Oct 1987). "Isolation and characterization of ...
4-hydroxyphenylpyruvate dioxygenase (EC *Category:EC 1.13.12 (With incorporation of one atom of oxygen (internal ... Catechol-O-methyl transferase EC *DNA methyltransferase EC, EC, EC ... Category:EC 1.3.2 (with a cytochrome as acceptor). *Category:EC 1.3.3 (with oxygen as acceptor) *Protoporphyrinogen oxidase EC ... Category:EC 1.4 (act on the CH-NH2 group of donors)Edit. *Category:EC 1.4.3 *Monoamine oxidase EC ...
... can be directly metabolized by catechol-O-methyl transferase to 3-O-methyldopa, and then further to vanillactic acid. ... 4-Hydroxyphenylpyruvate dioxygenase. *Homogentisate 1,2-dioxygenase. *Fumarylacetoacetate hydrolase. *tyrosine→melanin: ... L-DOPA (/ˌɛlˈdoʊpə/), also known as levodopa (/ˌlɛvoʊˈdoʊpə/) or L-3,4-dihydroxyphenylalanine is an amino acid that is made and ... 16 (3): 106-112. doi:10.1021/ar00087a006.. *^ "Synthetic scheme for total synthesis of DOPA, L- (Monsanto)". UW Madison, ...
The activity of tyrosinase is similar to catechol oxidase, a related class of copper oxidase. Tyrosinases and catechol oxidases ... Oxidoreductases: dioxygenases, including steroid hydroxylases (EC 1.14). 1.14.11: 2-oxoglutarate. *Prolyl hydroxylase *HIF ... In the presence of catechol, benzoquinone is formed (see reaction below). Hydrogens removed from catechol combine with oxygen ... doi:10.1016/0005-2744(80)90061-3. PMID 6766744.. *^ PDB: 1WX3​; Matoba Y, Kumagai T, Yamamoto A, Yoshitsu H, Sugiyama M (2006 ...
Catechol+2,3-dioxygenase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal. ... Catechol 2,3-dioxygenase (EC, 2,3-pyrocatechase, catechol 2,3-oxygenase, catechol oxygenase, metapyrocatechase, ... Kojima Y, Itada N, Hayaishi O (August 1961). "Metapyrocatachase: a new catechol-cleaving enzyme". The Journal of Biological ... is an enzyme with systematic name catechol:oxygen 2,3-oxidoreductase (decyclizing). This enzyme catalyses the following ...
What is catechol 2,3-dioxygenase? Meaning of catechol 2,3-dioxygenase medical term. What does catechol 2,3-dioxygenase mean? ... Looking for online definition of catechol 2,3-dioxygenase in the Medical Dictionary? catechol 2,3-dioxygenase explanation free ... catechol 2,3-dioxygenase. cat·e·chol 2,3-di·ox·y·gen·ase. (katĕ-kol dē-oksē-jenās), An oxidoreductase oxidizing catechol, ... a href=https://medical-dictionary.thefreedictionary.com/catechol+2%2c3-dioxygenase,catechol 2,3-dioxygenase,/a,. *Facebook ...
... the crystal structure of catechol 2,3-dioxygenase (metapyrocatechase) from Ppseudomonas putida mt-2. ... STRUCTURE OF CATECHOL 2,3-DIOXYGENASE (METAPYROCATECHASE) FROM PSEUDOMONAS PUTIDA MT-2. ...
CATECHOL. C6 H6 O2. YCIMNLLNPGFGHC-UHFFFAOYSA-N. Ligand Interaction. ... Crystal Structure of the Biphenyl-cleaving Extradiol Dioxygenase from a PCB-degrading Pseudomonad.. Han, S., Eltis, L.D.,& ... The steady-state cleavage of catechols by 2,3-dihydroxybiphenyl 1, 2-dioxygenase (DHBD), the extradiol dioxygenase of the ... The steady-state cleavage of catechols by 2,3-dihydroxybiphenyl 1, 2-dioxygenase (DHBD), the extradiol dioxygenase of the ...
Grouping of phenol hydroxylase and catechol 2,3-dioxygenase genes among phenol- and p-cresol-degrading Pseudomonas species and ... Grouping of phenol hydroxylase and catechol 2,3-dioxygenase genes among phenol- and p-cresol-degrading Pseudomonas species and ... Grouping of phenol hydroxylase and catechol 2,3-dioxygenase genes among phenol- and p-cresol-degrading Pseudomonas species and ... Merimaa, M.; Heinaru, E.; Liivak, M.; Vedler, E.; Heinaru, A. (2006). Grouping of phenol hydroxylase and catechol 2,3- ...
An archetypical extradiol-cleaving catecholic dioxygenase: the crystal structure of catechol 2,3-dioxygenase (metapyrocatechase ... c23o gene, encoding catechol 2,3-dioxygenase from Planococcus sp. strain S5 was randomly mutagenized to generate variant forms ... Huang SL, Hsu YC, Wu CM, Lynn JW, Li WH (2010) Thermal effects on the activity and structural conformation of catechol 2,3- ... George KW, Kagle J, Junker L, Risen A, Hay AG (2011) Growth of Pseudomonas putida F1 on styrene requires increased catechol-2,3 ...
The catechol degradation (Ctd) enzymes were identified based on the analysis of the JS42 genome sequence (). CtdE1, catechol 2, ... strain JS42 to 2-nitrotoluene.. Rabinovitch-Deere CA1, Parales RE.. Author information. 1. Department of Microbiology, College ... Fig 2. Wild-type JS42m responses in the qualitative capillary assay. Responses to buffer (negative control) or succinate (10 mM ... Fig 3. Chemotactic response to nitrite and generation of nitrite from 2NT during the qualitative capillary assay performed with ...
17O]Water and nitric oxide binding by protocatechuate 4,5-dioxygenase and catechol 2,3-dioxygenase. Evidence for binding of ... 17O]Water and nitric oxide binding by protocatechuate 4,5-dioxygenase and catechol 2,3-dioxygenase. Evidence for binding of ... 17O]Water and nitric oxide binding by protocatechuate 4,5-dioxygenase and catechol 2,3-dioxygenase. Evidence for binding of ... T1 - [17O]Water and nitric oxide binding by protocatechuate 4,5-dioxygenase and catechol 2,3-dioxygenase. Evidence for binding ...
IPR017624. Catechol_2-3_dOase. IPR029068. Glyas_Bleomycin-R_OHBP_Dase. IPR004360. Glyas_Fos-R_dOase_dom. IPR037523. VOC. ... Belongs to the extradiol ring-cleavage dioxygenase family.Curated. ,p>UniProtKB Keywords constitute a ,a href="http://www. ... IPR017624. Catechol_2-3_dOase. IPR029068. Glyas_Bleomycin-R_OHBP_Dase. IPR004360. Glyas_Fos-R_dOase_dom. IPR037523. VOC. ... Catechol + O2 = 2-hydroxymuconate-6-semialdehyde.. ,p>This subsection of the Function section provides information relevant ...
Crystallization and preliminary crystallographic analysis of the catechol 2,3-dioxygenase PheB from Bacillus stearothermophilus ... PheB, an extradiol-cleaving catecholic dioxygenase, was crystallized by the hanging-drop vapour-diffusion method using PEG 4000 ... The crystal belongs to the orthorhombic system, space group P212121, and diffracts to 2.3 Å resolution. ... The α-2,6-sialyltransferase PM0188 from P. multocida was purified using affinity-column chromatographic methods and ...
Catechol dioxygenases are metalloprotein enzymes that carry out the oxidative cleavage of catechols. This class of enzymes ... 4-dioxygenase (EC The active site of catechol dioxygenases most frequently contains iron, but manganese-containing ... Catechol dioxygenases belong to the class of oxidoreductases and have several different substrate specificities, including ... An example of the reaction carried out by catechol 1,2-dioxygenase is the formation of cis,cis-muconic acid from catechol, ...
The XylE (catechol 2,3-dioxygenase) assays were determined spectrophotometrically at 30°C in a total volume of 3 ml of 100 mM ... Catechol 2,3-dioxygenase assays.For enzymatic assays, overnight cultures were diluted 1:50 in 250 ml of tryptic soy broth ... aureus mutant strains and assayed for the activity of catechol 2,3-dioxygenase, an enzyme which is the gene product of thexylE ... were lysed with lysostaphin and assayed for catechol 2,3-dioxygenase activity as described previously. The average values for ...
... "catechol 2,3 dioxygenase" FT /transl_table="11" FT /note="KEGG: ajs:Ajs_0218 catechol 2,3-dioxygenase; FT TIGRFAM: catechol 2,3 ... dioxygenase; PFAM: FT Glyoxalase/bleomycin resistance protein/dioxygenase" FT /db_xref="GI:319761060" FT /db_xref="GO:0008198" ... HOGENOMDNA: ALDEN1_2.PE322. ALDEN1_2.PE322 Location/Qualifiers FT CDS_pept 359860..360786 FT /locus_tag="Alide_0330" FT /gene_ ...
Dioxygenases * Catechol 2,3-Dioxygenase Associated data * GENBANK/U03991 * GENBANK/U03992 * GENBANK/U17499 ...
catechol 2,3 dioxygenase [KO:K00446] [EC:]. Mrub_1083 Glyoxalase/bleomycin resistance protein/dioxygenase [KO:K07104 ... 4-dioxygenase, alpha subunit [KO:K00448] [EC:]. Mrub_2693 ... 3-hydroxyacyl-CoA dehydrogenase NAD-binding protein [KO:K07516] [EC:] ... 2-Hydroxycyclohexanecarboxyl coenzyme A dehydrogenase, an enzyme characteristic of the anaerobic benzoate degradation pathway ...
Catechol dioxygenase activity assays.Catechol dioxygenase (XylE) activity assays were performed as described in reference18. ... which encodes catechol dehydrogenase and converts colorless catechol to an intensely yellow oxidation product (hydroxymuconic ... Within orf261is the Sau3AI site of fusion of SLP1 to the xylEgene of pXE4, indicating that production of catechol dehydrogenase ... Appearance of yellow colonies was determined visually no more than 1 h after spreading of the catechol in the initial screen ...
From Catechol to 2-Hydroxy-cis,cis-muconate semialdehyde. Graphic of the reaction.. Medline reference Cerdan P, Wasserfallen A ... Catechol , , O2 catechol , / 2,3-dioxygenase ,/ , Search GenBank, 925 hits on Apr. 03, 2012 Kyoto ,\ ExPASy , \ , H+ ... Search Medline titles for catechol 2,3-dioxygenase. 77 citations on July 26, 2012. ... v 2-Hydroxy-cis,cis-muconate semialdehyde Display a pathway starting from this reaction. EAWAG-BBD Biotransformation rules in ...
... lysed to obtain cell lysate with catechol dioxygenase activity for determination of kinetic parameters of catechol dioxygenase ... Growth with Catechol (LB) The addition of catechol had distinctive effects on the XylE expressing cells growing in LB medium. ... The cleavage of the GFP by TEV protease, which is synthesised on arrival of the input signal, means that Catechol dioxygenase ... Reversion of the reaction profile back to the kinetics of the wild type catechol dioxygenase would mean that we have ...
... catechol 2,3-dioxygenase; COG2185, methylmalonyl-CoA mutase; COG1884, methylmalonyl-CoA mutase; and COG4869, propanediol ... 3) (19, 20). Since the carbohydrate supply in newly hatched chickens is limited and chickens up to the age of 3 or 4 days live ... 2, green box). The expression of l-fucose isomerase was also quite characteristic of Firmicutes, since it was found in ... 2). In Butyrivibrio and Subdoligranulum, we also detected expression of butyrate kinase, an enzyme involved in the last step of ...
... (meta ring cleavage). When cursor points to a box further details will be displayed in a tooltip window. If ... catechol biosynthesis. EC acetaldehyde dehydrogenase (acetylating). EC 2-hydroxymuconate-6-semialdehyde ... EC catechol 2,3-dioxygenase. EC 2-hydroxymuconate-6-semialdehyde hydrolase. EC 4-oxalocrotonate ... EC 4-hydroxy-2-oxovalerate aldolase. EC 2-oxopent-4-enoate hydratase. EC 2-hydroxymuconate ...
Molecular Characterization of Aniline Biodegradation by Some Bacterial Isolates having Unexpressed Catechol 2,3-Dioxygenase ... In this study, we fabricated hybrid [email protected][Fe.sub.3][O.sub.4] by the in situ aniline polymerization at the spherical of [Fe.sub.3 ... a colorless, oily, slightly water-soluble liquid, C6H5NH2, used chiefly in the synthesis of dyes and drugs. ... Silver nitrate (AgN[O.sub.3]) and aniline chloride (AnCl) were purchased from Sigma-Aldrich.. Spectroscopic Study of ...
... 87 endo-cleaving rubber dioxygenase. EC Accepted name: catechol 1,2-dioxygenase. Reaction: catechol + O2 ... EC sulfur dioxygenase. EC cysteamine dioxygenase. EC cysteine dioxygenase. EC now ... A novel non-heme iron-containing dioxygenase. Chloridazon-catechol dioxygenase from Phenylobacterium immobilis DSM 1986. Eur. J ... Other name(s): 10R-DOX; (10R)-dioxygenase; 10R-dioxygenase. Systematic name: linoleate:oxygen (10R)-oxidoreductase. Comments: ...
Insights into the binding interaction of substrate with catechol 2,3-dioxygenase from biophysics point of view. ... 3.. Bioinspired red blood cell membrane-encapsulated biomimetic nanoconstructs for synergistic and efficacious chemo- ... Paenibacillus polymyxa biofertilizer application in a tea plantation reduces soil N2O by changing denitrifier communities. ... A novel amino acid site closely associated with the neurovirulence of live, attenuated Japanese encephalitis vaccine (SA14-14-2 ...
To promote crosslinking, other enzymes also may be used, such as catechol 2,3-dioxygenase. FIG. 3 is an illustrative gel (SDS ... with the conversion of tyrosine to dopa occurring by the action of a catechol oxidase (a `tyrosinase` enzyme) present in the ... 2): (Xaa1 -Xaa1 -Xaa1 -Xaa2 -Xaa3 -Xaa2 -Xaa1 -Xaa1 -Xaa1 -Xaa1 -Xaa3 -Xaa2 -Xaa3 -Xaa1)b ... 2): (Xaa1 -Xaa1 -Xaa1 -Xaa2 -Xaa3 -Xaa2 -Xaa1 -Xaa1 -Xaa1 -Xaa1 -Xaa3 -Xaa2 -Xaa3 -Xaa1)b ...
Compound: Catechol 2,3-dioxygenase, Extradiol ring cleavage protein. Species: Diaphorobacter sp. DS2 [TaxId:1302548]. Database ... Description: Catechol 2,3-dioxygenase (C23O64) from Diaphorobacter sp DS2. Class: oxidoreductase. Keywords: Apo crystal of ... catechol 2, 3 dioxygenase, OXIDOREDUCTASE. Deposited on 2018-04-30, released 2019-05-22. The last revision prior to the SCOPe ...
Crystallization and Preliminary X-Ray Diffraction Studies of Expressed Pseudomonas putida Catechol 2, 3-Dioxygenase (1997) ... Crystallization and Preliminary X-Ray Diffraction Studies of Expressed Pseudomonas putida Catechol 2,3-Dioxygenase. (1997) ... Articles in CiNii:3. * 2P-021 High-resolution X-ray crystallography of Taka-amylase A and bovine H-protein : the crystals grown ... Structural insight into the stereoselective production of PGF_,2α, by Old Yellow Enzyme from Trypanosoma cruzi (2011) ...
Catechol-2,3-dioxygenase and β-galactosidase assays.Bacterial strains were grown overnight (for 12 to 14 h) in L broth ... The levels of transcription of the different reporter gene fusions were measured by assaying catechol-2,3-dioxygenase activity ... The levels of transcription of the different reporter gene fusions were measured by assaying catechol-2,3-dioxygenase activity ... and the levels of catechol-2,3-dioxygenase activity in the bacterial lysates were determined as described elsewhere (32). The ...
removal of four catechols (1,2-dihydroxybenzene), 4-chlorocatechol (4-CC), 4,5-dichlorocatechol (4,5-DCC) and 4-methylcatechol ... Escherichia coli harboring the DMS monooxygenase genes from strain 20B alone, or in combination with the cumene dioxygenase ... hexahydro-1,3,5-trinitro-1,3,5-triazine is widely used for military and commercial purposes due to its high explosive ... Hexahydro-1,3,5-trinitro-1,3,5-triazine and its degradation products are toxic, mutagenic and carcinogenic to humans and other ...
Catechol 2,3-dioxygenase functional in oxygen-limited (hypoxic) environments. Appl. Environ. Microbiol. 62:1728-1740. ... Cloning and expression of the benzoate dioxygenase genes from Rhodococcus sp. strain 19070. Appl. Environ. Microbiol. 67:2507- ... Amplified functional DNA restriction analysis to determine catechol 2,3-dioxygenase gene diversity in soil bacteria. J. ... and catechol 2,3-dioxygenase (XylE) were detected by PCR in BTEX-polluted groundwater samples (6) and were associated with ...
Purification and Characterization of Catechol 2,3-Dioxygenase from the Aniline Degradation Pathway of Acinetobacter sp. YAA and ... Biodegradation of 2,6-Naphthalenedisulfonic Acid by a Floc-Forming Bacterium, Pigmentiphaga sp. NDS-1. (2002) ... Biodegradation of 2,6-Naphthalenedisulfonic Acid by a Floc-Forming Bacterium, Pigmentiphaga sp. NDS-1 (2002) ... 3P-2008 Isolation and identification of 3-methyl-4-nitrophenol-degrading bacterium and its degradation pathway (2010) ...
  • Aoki H, Kimura T, Habe H, Yamane H, Kodama T, Omori T (1996) Cloning, nucleotide sequence, and characterization of the genes encoding enzymes involved in the degradation of cumene to 2-hydroxy-6-oxo-methylocta-2,4-dienoic acid in Pseudomonas fluoresens IPO1. (edu.pl)
  • Cerdan P, Wasserfallen A, Rekik M, Timmis KN, Harayama S (1994) Substrate specificity of catechol 2,3-dioxygenase encoded by TOL plasmid pWW0 of Pseudomonas putida and its relationship to cell growth. (edu.pl)
  • The Pseudomonas putida xylE gene, which encodes catechol 2,3-dioxygenase, is often used as a reporter to quantitate gene expression. (wikipedia.org)
  • The XylE gene, originating from the TOL plasmid of Pseudomonas putida , encodes the catechol 2,3-dioxygenase (C2,3O) enzyme, the final choice for the output signal mediator of our system. (igem.org)
  • Fujisawa H (1970) Protocatechuate 3,4-dioxygenase ( Pseudomonas ). (springer.com)
  • Nakazawa T, Yokota T (1973) Benzoate metabolism in Pseudomonas putida (arvilla) mt-2: demonstration of two benzoate pathways. (springer.com)
  • We have studied the well known oxygenase, catechol 1, 2-dioxygenase, the gene of which has been successfully cloned and we reported for the first time existence of 3 isozymes of this enzyme from a Pseudomonas. (nii.ac.jp)
  • Nucleotide sequence and expression of the catechol 2,3-dioxygenase-encoding gene of phenol-catabolizing Pseudomonas CF600. (diva-portal.org)
  • Twitching motility is a novel mode of flagellum-independent movement on solid surfaces and is also found in Pseudomonas aeruginosa ( 4 , 37 ) and Synechocystis species ( 2 ), as well as in Myxococcus xanthus , in which it is manifested as social gliding motility ( 43 ). (asm.org)
  • This work demonstrates the ring-cleavage pathways of catechol on Pseudomonas cepacia ATCC 29351, formed upon its growth on salicylate and benzoate, each as a sole carbon source. (springer.com)
  • Catechol 2,3-dioxygenase (xylE) is found in Pseudomonas putida and hydrolyzes catechol into 2-HMS, which can enter the citric acid cycle 6 . (igem.org)
  • This reaction has been observed in several subspecies of Pseudomonas putida, in Burkholderia cepacia, Sphingomonas and Bacillus stearothermophilus, in activated sludge microorganisms [A2397, E31, J654, K5], as well as being catalysed by chlorocatechol 2,3-dioxygenase and 1,2-dihydroxynaphthalene oxygenase (see below). (ormedmedical.us)
  • 3- and 4-alkylphenol degradation pathway in Pseudomonas sp. (microbiologyresearch.org)
  • 2-Nitrotoluene (2NT) degradation pathway in Acidovorax sp. (nih.gov)
  • Hexahydro-1,3,5-trinitro-1,3,5-triazine and its degradation products are toxic, mutagenic and carcinogenic to humans and other biological systems. (brenda-enzymes.org)
  • Most of the 2,4-D plasmids were found in strains isolated by enrichment on 2,4-D as the sole source of carbon and energy, and some of them were found to take part in the degradation of a herbicide with a similar structure, 2-methyI-4-chlorophenoxyacetic acid. (asmscience.org)
  • The simultaneous induction of catechol 1,2- and 2,3-dioxygenase is not detected in salicylate degradation. (springer.com)
  • Two other mutant classes which were blocked in toluene catabolism, TOM B(-), which lacked catechol-2,3-dioxygenase, and TOM C(-) which lacked 2-hydroxy-6-oxoheptadienoic acid hydrolase activity, were fully capable of TCE degradation. (epa.gov)
  • Catechol is a prime candidate to function as a hub for the funneling and subsequent degradation of other toxic compounds found in abundance, such as naphthene, anthracene, and fluorene into 2-HMS (Figure 1) 3,4,5 . (igem.org)
  • tmoA and related genes encode the alpha-subunit of the hydroxylase component of the major group (subgroup 1 of subfamily 2) of bacterial multicomponent mono-oxygenase enzyme complexes involved in aerobic benzene, toluene, ethylbenzene and xylene (BTEX) degradation. (hbo-kennisbank.nl)
  • Catalyzes the oxidation of catechol to 2-hydroxymuconate semialdehyde in the carbazole and BENZOATE degradation via HYDROXYLATION pathways. (bvsalud.org)
  • It also catalyzes the conversion of 3-methylcatechol to cis, cis-2-hydroxy-6-oxohept-2,4-dienoate in the TOLUENE and XYLENE degradation pathway. (bvsalud.org)
  • The enzyme initiates the meta- cleavage pathway of catechol degradation. (qmul.ac.uk)
  • The deletion of the gene META1_4251 encoding a putative acrylyl-CoA reductase led to reduced degradation rate of 3-HP in late stationary phase. (biomedcentral.com)
  • Furthermore, we showed that a reductive route coupled with the ethylmalonyl-CoA pathway was the major channel responsible for degradation of the 3-HP during the growth transition. (biomedcentral.com)
  • This approach have already been done in others studies although using other genes codifying for same catechol degradation approach. (recentpatentsonbiotechnology.com)
  • c23o gene, encoding catechol 2,3-dioxygenase from Planococcus sp. (edu.pl)
  • Characterization of the 4-carboxy-4-hydroxy-2-oxoadipate aldolase gene and operon structure of the protocatechuate 4,5-cleavage pathway genes in Sphingomonas paucimobilis SYK-6. (genome.jp)
  • Colonies of cells that express XylE gene product become yellow within seconds after selection plates are sprayed with catechol, a colorless substrate, that is converted by C2,3O to the yellow product, 2-hydroxymuconic semialdehyde. (igem.org)
  • A spectrophotometric assay was prepared, where the spectra of two cultures of E.coli (one XylE gene transformed and the other not) were compared on addition of 0.1mM Catechol substrate. (igem.org)
  • A number of environmental cues are known to affect type III secretion-associated gene expression ( 3 , 4 , 13 , 18 , 35 , 41 , 42 ). (asm.org)
  • The minimum number of clones that need to be screened in order to find a gene of interest in the metagenomic library increases with the presence of eukaryotic DNA (a eukaryotic genome is 3 to 140,000 Mbp compared to prokaryotic organisms of 0.6 to 9.5 Mbp). (asmscience.org)
  • A BamHI cassette carrying the catechol 2,3-dioxygenase (C230) gene, tdnC, fused to the nopaline-inducible Agrobacterium promoter Pi 2[noc] was constructed. (eurekamag.com)
  • One-year monitoring of meta-cleavage dioxygenase gene expression and microbial community dynamics reveals the relevance of subfamily I.2.C extradiol dioxygenases in hypoxic, BTEX-contaminated groundwater , SYSTEMATIC AND APPLIED MICROBIOLOGY 36: (5) pp. 339-350. (doktori.hu)
  • Annotation described in this paper also reveals evidence for several metabolic pathways that have yet to be observed experimentally, including a sulphur oxidation ( sox FCDYZAXB) gene cluster, Calvin cycle enzymes, and proteins involved in nitrogen fixation in other species (including RubisCo, ribulose-phosphate 3-epimerase, and nif gene families, respectively). (biomedcentral.com)
  • Examples of recent gene duplication events in signaling as well as dioxygenase clusters are present, indicating selective gene family expansion as a relatively recent event in D. aromatica 's evolutionary history. (biomedcentral.com)
  • lapB , the first gene in the lap operon, encodes catechol 2,3-dioxygenase (C23O). (microbiologyresearch.org)
  • Here we constructed a malonyl-CoA pathway by heterologously overexpressing the mcr gene to convert methanol into 3-HP in M. extorquens AM1. (biomedcentral.com)
  • The engineered strains demonstrated 3-HP production with initial titer of 6.8 mg/l in shake flask cultivation, which was further improved to 69.8 mg/l by increasing the strength of promoter and mcr gene copy number. (biomedcentral.com)
  • The steady-state cleavage of catechols by 2,3-dihydroxybiphenyl 1, 2-dioxygenase (DHBD), the extradiol dioxygenase of the biphenyl biodegradation pathway, was investigated using a highly active, anaerobically purified preparation of enzyme. (rcsb.org)
  • Catechol dioxygenases are metalloprotein enzymes that carry out the oxidative cleavage of catechols. (wikipedia.org)
  • Catechol 2,3-dioxygenase and other meta-cleavage catabolic pathway genes in the 'anaerobic' termite gut spirochete Treponema primitia . (caltech.edu)
  • A. chroococcum expressed only ortho cleavage dioxygenases during growth on naphthalene and 4-toluate and only meta cleavage dioxygenases on the other aromatics. (springer.com)
  • B. mobilis expressed only ortho cleavage dioxygenases. (springer.com)
  • Catechol 1,2-dioxygenase (CTD) and protocatechuate 3,4-dioxygenase (PCD) are bacterial non-heme iron enzymes, which catalyse the oxidative cleavage of catechols to cis, cis -muconic acids with the incorporation of molecular oxygen via a mechanism involving a high-spin ferric centre. (ias.ac.in)
  • The nature of heterocyclic rings of the ligands and the methyl substituents on them regulate the electronic spectral features, Fe III /Fe II redox potentials and catechol cleavage activity of the complexes. (ias.ac.in)
  • Four of the complexes catalyze the oxidative cleavage of H 2 DBC by molecular oxygen to yield intradiol cleavage products. (ias.ac.in)
  • The lower pathway includes cleavage of the aromatic ring by dioxygenases with formation of (chloro)maleylacetates. (asmscience.org)
  • C23O preferentially oxidizes 4-alkylcatechols via proximal (2,3) cleavage. (microbiologyresearch.org)
  • Two key metabolites of the meta-cleavage pathway, 5-formyl-2-hydroxy-6,6,8,8-tetramethylnona-2,4-dienoic acid and 6,6,8,8-tetramethyl-2-oxonon-4-enoic acid, were detected by LC-ESI-Q-TOF. (ncu.edu.tw)
  • The present study suggested that the multistep conversion by both ring hydroxylase and cleavage dioxygenase should be potential in the synthesis of industrial precursors and provide a novel avenue in the wastewater recycling treatment. (sigmaaldrich.com)
  • Jones DCN, Cooper RA (1990) Catabolism of 3-hydroxybenzoate by the gentisate pathway in Klebsiella pneumoniae M5a1. (springer.com)
  • The catechol 2,3-dioxygenase (C23O) enzyme of this pathway catalyses the conversion of catechol to 2-hydroxymuconic semialdehyde. (diva-portal.org)
  • When grown on salicylate, P. cepacia induces only the catechol ortho pathway by its induction of catechol 1,2-dioxygenase. (springer.com)
  • Although catechol is the metabolic intermediate for both salicylate and benzoate, catechol did not induce either pathway when used as a sole carbon source. (springer.com)
  • The pathway involves conversion of toluene via o-cresol to 3-methylcatechol. (epa.gov)
  • The GC-MS data also shows the presence of catechol and 2-hydroxymuconic semialdehyde , which confirms the established pathway of benzene biodegradation. (thefreedictionary.com)
  • Instead, the strain contained catechol 1,2-dioxygenase and the alpha/beta subunits of protocatechuate 3,4-dioxygenase, indicating use of the β-ketoadipate pathway to degrade PHE and related aromatic compounds. (lancs.ac.uk)
  • Only has the pathway for BT biodegradation been proposed based on the identification of intermediate molecules (Fig. 3). (kenyon.edu)
  • In vitro enzymatic assay and β-alanine pathway dependent 13 C-labeling further demonstrated that a reductive route sequentially converted 3-HP-CoA to acrylyl-CoA and propionyl-CoA, with the latter being reassimilated into the ethylmalonyl-CoA pathway. (biomedcentral.com)
  • We demonstrated the feasibility of constructing the malonyl-CoA pathway in M. extorquens AM1 to generate 3-HP. (biomedcentral.com)
  • 17O]Water and nitric oxide binding by protocatechuate 4,5-dioxygenase and catechol 2,3-dioxygenase. (elsevier.com)
  • When grown on substrates typically converted to protocatechuate, inducible protocatechuate 3,4-dioxygenase and/or protocatechuate 4,5-dioxygenase was expressed. (springer.com)
  • Andujar E, Santero E (2003) Site-directed mutagenesis of an extradiol dioxygenase involved in tetralin biodegradation identifies residues important for activity or substrate specificity. (edu.pl)
  • Biodegradation of 2,6-Naphthalenedisulfonic Acid by a Floc-Forming Bacterium, Pigmentiphaga sp. (nii.ac.jp)
  • Biodegradation has long been seen as a cost-effective and ecological way to eliminate environmental contamination [ 2 ]. (hindawi.com)
  • However, interestingly, benzoate-grown cells induce the ortho and meta pathways for the biodegradation of catechol, by inducing simultaneously catechol 1,2-dioxygenase and 2,3-dioxygenase, respectively, in the ratio of 7:1. (springer.com)
  • The genome sequence of R.pyridinivorans strain AK37 has identified several key enzymes involved in the six pathways of monocyclic aromatic compound biodegradation: protocatechuate 3,4-dioxygenase, benzoate 1,2-dioxygenase, 3-ketosteroid-9α-hydroxylase, 3-ketosteroid-σ-dehydrogenase, and so on. (kenyon.edu)
  • International Journal of Environmental Bioremediation & Biodegradation , 2014 2 (2), pp 75-83. (sciepub.com)
  • The cells grown on benzoate and catechol showed mainly catechol 1,2- dioxygenase activity. (bireme.br)
  • 2. Role of oxygen in the metabolic regulation : Besides the well known … More role of oxygen in the energy metabolism, oxygen has been shown to play an important physiological role in metabolic regulation through oxygenase-catalyzed reactions. (nii.ac.jp)
  • For the elucidation of the active site structure of another well characterized oxygenase, catechol 2, 3 - dioxygense, we used o-nitrophenol, a competitive inhibitor, and found it a useful active site probe for this enzyme. (nii.ac.jp)
  • 1,2-dihydroxynaphthalene dioxygenase ( EC , 1,2-DHN dioxygenase , DHNDO , 1,2-dihydroxynaphthalene oxygenase , 1,2-dihydroxynaphthalene:oxygen oxidoreductase ) is an enzyme with systematic name naphthalene-1,2-diol:oxygen oxidoreductase . (wikipedia.org)
  • Strain CAR-SF composed of terminal oxygenase component CarAa, ferredoxin component CarAc, and ferredoxinreductase component CarAd.Expression vector encoding carbazole 1,9a-dioxygenase (CARDO) from Neptuniibacter sp. (elsevier.com)
  • spdB1 and spdB2 , two overlapping genes immediately 3′ to P op imp and directly regulated by imp , are shown by Tn 5 mutagenesis to control transfer-associated growth inhibition (i.e., pocking). (asm.org)
  • The genes coding for these enzymes are located on pVI150, an IncP-2 degradative mega plasmid of this strain. (diva-portal.org)
  • Mutations in the highly conserved pilT genes encoding members of a large family of putative nucleotide binding proteins (TrbB-like proteins) lead to a piliated but nonmotile phenotype in all four organisms ( 2 , 37 , 39 , 42 ). (asm.org)
  • Alfreider A, Vogt C, Babel W (2003) Expression of chlorocatechol 1,2-dioxygenase and chlorocatechol 2,3-dioxygenase genes in chlorobenzene-contaminated subsurface samples. (springer.com)
  • RT-PCR analyses of total RNA from both non-induced and putatively induced cultures conducted in STR showed that the transcript levels of only one out of two constitutively expressed laccase genes were markedly increased by the presence of 0.25 mM 2,5-xylidine in the optimised medium. (chemweb.com)
  • The lap genes are located in a 13·2 kb region with 14 ORFs in the order lapRBKLMNOPCEHIFG and with the same transcriptional orientation. (microbiologyresearch.org)
  • The lapKLMNOP and lapCEHIFG genes encode a multicomponent phenol hydroxylase (mPH) and enzymes that degrade derivatives of 2-hydroxymuconic semialdehyde (HMS) to TCA cycle intermediates, respectively. (microbiologyresearch.org)
  • strain JS42 to 2-nitrotoluene. (nih.gov)
  • strain JS42 is able to utilize 2-nitrotoluene (2NT) as its sole carbon, nitrogen, and energy source. (nih.gov)
  • Strain KB2 is from among different Stenotrophomonas maltophilia strains the first one described as exhibiting the activities of three types of dioxygenases depending on the structure of the inducer. (bireme.br)
  • Protocatechuate 3,4-dioxygenase was found in crude cell extracts of this strain after incubation with 4-hydroxybenzoic acid, protocatechuic acid and vanillic acid. (bireme.br)
  • Because of broad spectrum of dioxygenases' types that Stenotrophomonas maltophilia KB2 can exhibit, this strain appears to be very powerful and useful tool in the biotreatment of wastewaters and in soil decontamination. (bireme.br)
  • Strain CAR-SF CARDO, pETCARA1 was constructed and dioxygenase activity was assessed by monitoring the blue-indigo production in Luria broth media and SDS-PAGE. (elsevier.com)
  • The 16S rDNA analysis revealed 99% nucleotide similarity to that of the type strain of R.rhodochrous (Fig. 2) ( 10 ). (kenyon.edu)
  • The mycobacillin-sensitive Aspergillus niger strain G 3 Br and resistant mutants of it did not show any differences in their total lipid content, although the amounts of phospholipids and sterols, particularly phosphatidylcholine and cholesterol, were lower in resistant cells. (microbiologyresearch.org)
  • In vivo metabolic analysis showed a significant decrease of the acetyl-CoA pool size in the strain with the highest 3-HP titer, suggesting the supply of acetyl-CoA is a potential bottleneck for further improvement. (biomedcentral.com)
  • For the second-step conversion, the formation rates of semialdehydes by strain BphC(LA-4) were as follows: 5-fluoro-HODA>5-chloro-HODA>2-hydroxy-5-nitro-ODA>5-bromo-HODA>2-hydroxy-5-methyl-ODA. (sigmaaldrich.com)
  • The NH 2 -terminal sequence (35 residues) has been determined and shows 50% identity with other extradiol dioxygenases. (bl.uk)
  • The structural characterisation of 3-methylcatechol 2,3-dioxygenase at the primary, secondary and quaternary levels indicates that the enzyme is typical of the extradiol dioxygenases. (bl.uk)
  • Bugg TDH (2003) Dioxygenase enzymes: catalytic mechanisms and chemical models. (edu.pl)
  • In our laboratory iron(III) complexes of mono- and bisphenolate ligands have been studied successfully as structural and functional models for the intradiol-cleaving catechol dioxygenase enzymes. (ias.ac.in)
  • Altering substrate specificity of catechol 2,3-dioxygenase from Planococcus sp. (edu.pl)
  • Purification, biochemical properties and substrate specificity of a catechol 1,2-dioxygenase from a phenol degrading Acinetobacter radioresistens. (semanticscholar.org)
  • The substrate specificity for this enzyme was somewhat different from that seen for other catechol 2,3-dioxygenases, with 3-methylcatechol being cleaved at the highest rate. (bl.uk)
  • Substrate specificity of angular dioxygenase from carbazole-degrading bacterium Neptuniibacter sp. (elsevier.com)
  • Substrate specificity studies show that mPH preferentially oxidizes 3- and 4-alkylphenols to 4-alkylcatechols. (microbiologyresearch.org)
  • Cristobal S, Zemla A, Fischer D, Rychlewski L, Elofsson A (2001) A study of quality measures for protein threading models BMC Bioinformatics 2: 5. (edu.pl)
  • It has been well established in systems involving Tfp biogenesis and extracellular protein secretion that mutations in the Walker A consensus sequence (GXXXXGKT/S) and other conserved domains lead to loss of function ( 3 , 22 , 34 ). (asm.org)
  • The less related BfpF protein of enteropathogenic strains of Escherichia coli appears to be orthologous to PilT since bfpF mutants continue to express Tfp (referred to as bundle-forming pili [Bfp]) but are defective in bacterial aggregate dispersal, an apparently physiological equivalent of twitching motility ( 3 ). (asm.org)
  • In recent years, 2-D electrophoresis has been widely accepted as a standard procedure to separate complex protein mixtures in proteome studies ( Proteomics ). (wikipedia.org)
  • With the medium thus optimised, further experiments were performed in a 3-L stirred-tank reactor (STR) leading to a laccase activity of 2200 IU/L on day 9 with a productivity of 10.2 IU/(L h) and a specific activity of 26.7 IU/mg protein. (chemweb.com)
  • Promoter engineering for the recombinant protein production in prokaryotic systems[J]. AIMS Bioengineering, 2020, 7(2): 62-81. (aimspress.com)
  • Genetically engineered E. coli that encoded catechol 2,3- dioxygenase (C230) was created and investigated its ability to biodecomposition of phenanthrene and pyrene in spiked soil using high-performance liquid chromatography (HPLC) measurement. (recentpatentsonbiotechnology.com)
  • Hamzah RY, Tu S-C (1981) Determination of the position of mono-oxygenation in the formation of catechol catalyzed by salicylate hydroxylase. (springer.com)
  • Multistep conversion of para-substituted phenols by phenol hydroxylase and 2,3-dihydroxybiphenyl 1,2-dioxygenase. (sigmaaldrich.com)
  • A multistep conversion system of para-substituted phenols by recombinant phenol hydroxylase (PH(IND)) and 2,3-dihydroxybiphenyl 1,2-dioxygenase (BphC(LA-4)) was constructed in this study. (sigmaaldrich.com)
  • 1. Hayaishi, O. Direct oxygenation by O 2 , oxygenases. (qmul.ac.uk)
  • In air-saturated buffer, the partition ratios of catecholic substrates substituted at C-3 were inversely related to their apparent specificity constants. (rcsb.org)
  • In contrast, crystal structures of complexes with catechol and 3-methylcatechol revealed relationships between the binding of these smaller substrates and t-butanol that are consistent with the observed competitive inhibition. (rcsb.org)
  • All six species growing on substrates typically converted to catechol expressed inducible catechol 1,2-dioxygenase and/or catechol 2,3-dioxygenase. (springer.com)
  • Typical saturation kinetics were observed for catechol, 3-methylcatechol and 4-methylcatechol as substrates. (bl.uk)
  • The K m values for the organic substrates were all around 0.3 μM, the lowest found for any dioxygenase to date. (bl.uk)
  • Synthetic 6-aryl-2-hydroxy-6-ketohexa-2,4-dienoic acid substrates for C-C hydrolase BphD: investigation of a general base catalytic mechanism. (warwick.ac.uk)
  • Further classification into mono- and dioxygenases in based on whether the enzyme causes transfer of just one atom to the substrate (S) (eq. 1) or whether the whole molecule is incorporated into a single substrate (eq. 2a) or is shared by a pair of substrates (S, S′) (eq. 2b) ( 1, 2 ) 1 . (springer.com)
  • Other catechols are also substrates [J903]. (ormedmedical.us)
  • Catechol, 3- and 4-methylcatechol are poor substrates [G771]. (ormedmedical.us)
  • Docking studies with different para-substituted phenols and corresponding catechols inside of the active site of PH(IND) and BphC(LA-4) predicted that all the substrates should be transformed. (sigmaaldrich.com)
  • however, some fundamental questions remain, including (1) how much microbial biomass is present in the oceanic crust, (2) where do the microorganisms originate, and (3) what are their metabolic functions. (frontiersin.org)
  • There are two major routes that have been developed for 3-HP synthesis in engineered microorganisms including Escherichia coli , Saccharomyces cerevisiae and Pyrococcus furiosus . (biomedcentral.com)
  • In 2010, the Lethbridge iGEM team confirmed that xylE when introduce to E. coli hydrolyzes catechol to 2-HMS demonstrating and that this critical step can successfully be performed to convert toxic chemicals to metabolic intermediates 7 . (igem.org)
  • By-products of metabolism of certain microbiota members provide epithelial cells with butyrate, i.e., the most preferred energy source ( 3 ), and simultaneously suppress the expression of virulence factors of pathogens ( 4 ). (asm.org)
  • Mutational Analysis of the Metabolism of 2,6-Naphthalenedisulfonate by Pigmentiphaga sp. (nii.ac.jp)
  • Studies of Porphyrin Metabolism, 3. (unt.edu)
  • It is noteworthy that in nature 3-HP serves in central metabolism either as an intermediate of C3/C4 assimilation cycles or as an input carbon source in some bacteria and archaea. (biomedcentral.com)
  • The purpose of this study was purification and characterization of catechol 1,2-dioxygenase from Geobacillus sp. (semanticscholar.org)
  • Stages 2 and 3: Characterization of Mixed Cultures. (ufl.edu)
  • Journal of Applied & Environmental Microbiology , 2014 2 (5), pp 231-236. (sciepub.com)
  • Journal of Applied & Environmental Microbiology , 2 (5), 231-236. (sciepub.com)
  • Following the complete sequencing of the Escherichia coli genome, it has been shown that the proposed second citrate synthase of this organism, recently described by the authors, is in fact a 2-methylcitrate synthase that possesses citrate synthase activity as a minor component. (microbiologyresearch.org)
  • Whereas the hexameric citrate synthase is constitutively produced, the 2-methylcitrate synthase is induced during growth on propionate, and the catabolism of propionate to succinate and pyruvate via 2-methylcitrate is proposed. (microbiologyresearch.org)
  • The citrate synthases of the psychrotolerant eubacterium DS2-3R, and of the thermophilic archaea Thermoplasma acidophilum and Pyrococcus furiosus , are approximately 40% identical in sequence to the Escherichia coli 2-methylcitrate synthase andalso possess 2-methylcitrate synthase activity. (microbiologyresearch.org)
  • The data are discussed with respect tothe structure, function and evolution of citrate synthase and 2-methylcitrate synthase. (microbiologyresearch.org)
  • in the degradative pathways of 2-aminobenzenesulphonic, benzenesulphonic and 4-toluenesulphonic acids in Alcaligenes sp. (wikipedia.org)
  • To address the decontamination of (1) toxic organic compounds such as naphthene and catechol found in the tailings ponds, natural pathways that can break down the toxic chemicals can be utilized. (igem.org)
  • Although Escherichia coli or Saccharomyces cerevisiae are the primary industrial microbes for the production of 3-HP, alternative engineered hosts have the potential to generate 3-HP from other carbon feedstocks. (biomedcentral.com)
  • Chen YP, Lovell CR (1990) Purification and properties of catechol 1,2-dioxygenase from Rhizobium leguminosarum biovar viceae USDA 2370. (springer.com)
  • A purification procedure has been developed for a novel extradiol dioxygenase, designated as 3-methylcatechol 2,3-dioxygenase. (bl.uk)
  • We have employed a suite of molecular, bioinformatics, and biochemical tools to interrogate the thermodynamically limiting steps of H{sub 2} production from fatty acids in syntrophic communities. (unt.edu)
  • Molecular mechanisms of the involvement of ceramide in apoptosis in pancreatic β-cells and its role in the formation of insulin resistance in pathogenesis of type 2 diabetes are reviewed. (chemweb.com)
  • This enzyme catalyses the following chemical reaction catechol + O2 ⇌ {\displaystyle \rightleftharpoons } 2-hydroxymuconate semialdehyde This enzyme contains Fe(II). (wikipedia.org)
  • An example of the reaction carried out by catechol 1,2-dioxygenase is the formation of cis,cis-muconic acid from catechol, shown below. (wikipedia.org)
  • The absorbance at this particular wavelength is by the product of the C2,3O reaction which is called 2-hydroxymuconic semialdehyde and is what causes the yellow output. (igem.org)
  • Six species of free-living nitrogen fixing bacteria, Azomonas agilis, Azospirillum brasilense, Azospirillum lipoferum, Azotobacter chroococcum, Azotobacter vinelandii , and Beijerinckia mobilis , were surveyed for their ability to grow and fix N 2 using aromatic compounds as sole carbon and energy source. (springer.com)
  • Dorn E, Knackmuss H-J (1978) Chemical structure and biodegradability of halogenated aromatic compounds: two catechol 1,2-dioxygenases from a 3-chlorobenzoate grown pseudomonad. (springer.com)
  • Oxygenation and spontaneous deamination of 2-aminobenzenesulphonic acid in Alcaligenes sp. (wikipedia.org)
  • The presence of Catechol-1,2-dioxygenase (C12D) or Catechol-2,3-dioxygenase (C23D) was detected by the formation of products cis-cis muconic acid and 2-hydroxymuconic semialdehyde (2-HMS) at 260 nm and 375 nm, respectively (Santos and Linardi, 2004). (thefreedictionary.com)
  • 3-Hydroxypropionic acid (3-HP) is an important platform chemical, serving as a precursor for a wide range of industrial applications such as the production of acrylic acid and 1,3-propanediol. (biomedcentral.com)
  • Carbazole 1,9a-dioxygenase (CARDO) from Neptuniibacter sp. (elsevier.com)
  • The impact of five crucial variables on enzyme production, including glucose and nitrogen concentrations and three distinct putative inducers, namely, copper sulphate, 2,5-xylidine and olive-mill wastewater was thoroughly investigated. (chemweb.com)
  • The maximal laccase activity (about 2 IU/mL on day 11) was observed in the medium containing glucose and nitrogen at concentrations of 12.5 g/L and 20 mM, respectively, and 0.25 mM 2,5-xylidine as the sole inducer. (chemweb.com)
  • Mutants of the phenotypic class designated TOM A(-) were all defective in their ability to oxidize toluene, o-cresol, m-cresol, and phenol, suggesting that a single enzyme is responsible for conversion of these compounds to their hydroxylated products (3-methylcatechol from toluene, o-cresol, and m-cresol and catechol from phenol) in the wild type. (epa.gov)