A group of 1,2-benzenediols that contain the general formula R-C6H5O2.
Catalyzes the oxidation of catechol to 2-hydroxymuconate semialdehyde in the carbazole and BENZOATE degradation via HYDROXYLATION pathways. It also catalyzes the conversion of 3-methylcatechol to cis, cis-2-hydroxy-6-oxohept-2,4-dienoate in the TOLUENE and XYLENE degradation pathway. This enzyme was formerly characterized as EC 1.13.1.2.
Non-heme iron-containing enzymes that incorporate two atoms of OXYGEN into the substrate. They are important in biosynthesis of FLAVONOIDS; GIBBERELLINS; and HYOSCYAMINE; and for degradation of AROMATIC HYDROCARBONS.
An enzyme that catalyzes the oxidation of catechol to muconic acid with the use of Fe3+ as a cofactor. This enzyme was formerly characterized as EC 1.13.1.1 and EC 1.99.2.2.
Oxidases that specifically introduce DIOXYGEN-derived oxygen atoms into a variety of organic molecules.
2- or 4-Hydroxyestrogens. Substances that are physiologically active in mammals, especially in the control of gonadotropin secretion. Physiological activity can be ascribed to either an estrogenic action or interaction with the catecholaminergic system.
An enzyme that catalyzes the conversion of 4-hydroxyphenylpyruvate plus oxygen to homogentisic acid and carbon dioxide. EC 1.13.11.27.
An enzyme that catalyzes the oxidation of protocatechuate to 3-carboxy-cis-cis-muconate in the presence of molecular oxygen. It contains ferric ion. EC 1.13.11.3.
An enzyme that catalyzes the conversion of L-CYSTEINE to 3-sulfinoalanine (3-sulfino-L-alanine) in the CYSTEINE metabolism and TAURINE and hypotaurine metabolic pathways.
A dioxygenase with specificity for the oxidation of the indoleamine ring of TRYPTOPHAN. It is a LIVER-specific enzyme that is the first and rate limiting enzyme in the kynurenine pathway of TRYPTOPHAN catabolism.
Enzyme that catalyzes the movement of a methyl group from S-adenosylmethionone to a catechol or a catecholamine.
A mononuclear Fe(II)-dependent oxygenase, this enzyme catalyzes the conversion of homogentisate to 4-maleylacetoacetate, the third step in the pathway for the catabolism of TYROSINE. Deficiency in the enzyme causes ALKAPTONURIA, an autosomal recessive disorder, characterized by homogentisic aciduria, OCHRONOSIS and ARTHRITIS. This enzyme was formerly characterized as EC 1.13.1.5 and EC 1.99.2.5.
An enzyme that catalyzes the conversion of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde. It was formerly characterized as EC 1.13.1.6.
A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.
Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.
A species of gram-negative, aerobic bacteria isolated from soil and water as well as clinical specimens. Occasionally it is an opportunistic pathogen.
Benzoate derivatives substituted by one or more hydroxy groups in any position on the benzene ring.
A bacterial genus of the order ACTINOMYCETALES.
An antiseptic and disinfectant aromatic alcohol.
Mold and yeast inhibitor. Used as a fungistatic agent for foods, especially cheeses.
An enzyme of the oxidoreductase class that catalyzes the reaction between catechol and oxygen to yield benzoquinone and water. It is a complex of copper-containing proteins that acts also on a variety of substituted catechols. EC 1.10.3.1.
An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals.
Benzoic acid or benzoic acid esters substituted with one or more chlorine atoms.
A widely used industrial solvent.
Derivatives of BENZOIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxybenzene structure.
Widely distributed enzymes that carry out oxidation-reduction reactions in which one atom of the oxygen molecule is incorporated into the organic substrate; the other oxygen atom is reduced and combined with hydrogen ions to form water. They are also known as monooxygenases or hydroxylases. These reactions require two substrates as reductants for each of the two oxygen atoms. There are different classes of monooxygenases depending on the type of hydrogen-providing cosubstrate (COENZYMES) required in the mixed-function oxidation.
A genus of gram-negative, aerobic, rod-shaped bacteria characterized by an outer membrane that contains glycosphingolipids but lacks lipopolysaccharide. They have the ability to degrade a broad range of substituted aromatic compounds.
A genus of gram-negative, aerobic, rod-shaped bacteria. Organisms in this genus had originally been classified as members of the PSEUDOMONAS genus but overwhelming biochemical and chemical findings indicated the need to separate them from other Pseudomonas species, and hence, this new genus was created.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A fungistatic compound that is widely used as a food preservative. It is conjugated to GLYCINE in the liver and excreted as hippuric acid.
Salts and esters of gentisic acid.
A genus of gram-negative, aerobic, motile bacteria that occur in water and soil. Some are common inhabitants of the intestinal tract of vertebrates. These bacteria occasionally cause opportunistic infections in humans.
Toxic, volatile, flammable liquid hydrocarbon byproduct of coal distillation. It is used as an industrial solvent in paints, varnishes, lacquer thinners, gasoline, etc. Benzene causes central nervous system damage acutely and bone marrow damage chronically and is carcinogenic. It was formerly used as parasiticide.
Derivatives of adipic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a 1,6-carboxy terminated aliphatic structure.
An estrogenic steroid produced by HORSES. It has a total of five double bonds in the A- and B-ring. High concentration of equilenin is found in the URINE of pregnant mares.
A genus of gram-negative, straight or slightly curved rods which are motile by polar flagella and which accumulate poly-beta-hydroxybutyrate within the cells.
The functional hereditary units of BACTERIA.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.
A trihydroxybenzene or dihydroxy phenol that can be prepared by heating GALLIC ACID.
A gram-positive organism found in dairy products, fresh and salt water, marine organisms, insects, and decaying organic matter.
Organic compounds containing carbon and hydrogen in the form of an unsaturated, usually hexagonal ring structure. The compounds can be single ring, or double, triple, or multiple fused rings.
Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.
A genus in the family BURKHOLDERIACEAE, comprised of many species. They are associated with a variety of infections including MENINGITIS; PERITONITIS; and URINARY TRACT INFECTIONS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A genus of coccoid bacteria in the family PLANOCOCCACEAE. They are widely distributed in various habitats including sea water, freshwater ponds, cyanobacterial mats, and in marine animals.
A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.
Placing of a hydroxyl group on a compound in a position where one did not exist before. (Stedman, 26th ed)
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Methyl, propyl, butyl, and ethyl esters of p-hydroxybenzoic acid. They have been approved by the FDA as antimicrobial agents for foods and pharmaceuticals. (From Hawley's Condensed Chemical Dictionary, 11th ed, p872)
Industrial products consisting of a mixture of chlorinated biphenyl congeners and isomers. These compounds are highly lipophilic and tend to accumulate in fat stores of animals. Many of these compounds are considered toxic and potential environmental pollutants.
A group of gram-negative bacteria consisting of rod- and coccus-shaped cells. They are both aerobic (able to grow under an air atmosphere) and microaerophilic (grow better in low concentrations of oxygen) under nitrogen-fixing conditions but, when supplied with a source of fixed nitrogen, they grow as aerobes.
Iron-containing proteins that transfer electrons, usually at a low potential, to flavoproteins; the iron is not present as in heme. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
The rate dynamics in chemical or physical systems.
A family of gram negative, aerobic, non-sporeforming, rod-shaped bacteria.
An oxidation product of tryptophan metabolism. It may be a free radical scavenger and a carcinogen.
An enzyme that catalyzes the HYDROXYLATION of gamma-butyrobetaine to L-CARNITINE. It is the last enzyme in the biosynthetic pathway of L-CARNITINE and is dependent on alpha-ketoglutarate; IRON; ASCORBIC ACID; and OXYGEN.
A genus of asporogenous bacteria isolated from soil that displays a distinctive rod-coccus growth cycle.
An inborn error of amino acid metabolism resulting from a defect in the enzyme HOMOGENTISATE 1,2-DIOXYGENASE, an enzyme involved in the breakdown of PHENYLALANINE and TYROSINE. It is characterized by accumulation of HOMOGENTISIC ACID in the urine, OCHRONOSIS in various tissues, and ARTHRITIS.
A family of isomeric, colorless aromatic hydrocarbon liquids, that contain the general formula C6H4(CH3)2. They are produced by the destructive distillation of coal or by the catalytic reforming of petroleum naphthenic fractions. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
A family of compounds containing an oxo group with the general structure of 1,5-pentanedioic acid. (From Lehninger, Principles of Biochemistry, 1982, p442)
The salts or esters of salicylic acids, or salicylate esters of an organic acid. Some of these have analgesic, antipyretic, and anti-inflammatory activities by inhibiting prostaglandin synthesis.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Enzymes of the isomerase class that catalyze reactions in which a group can be regarded as eliminated from one part of a molecule, leaving a double bond, while remaining covalently attached to the molecule. (From Enzyme Nomenclature, 1992) EC 5.5.
Inorganic or organic compounds that contain divalent iron.
An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.
Benzoic acids, salts, or esters that contain an amino group attached to carbon number 2 or 6 of the benzene ring structure.
A flavoring agent. It is the intermediate product in the two-step bioconversion of ferulic acid to vanillin. (J Biotechnol 1996;50(2-3):107-13).
The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.
Proteins found in any species of bacterium.
Aminobenzenesulfonic acids. Organic acids that are used in the manufacture of dyes and organic chemicals and as reagents.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A group of proteins possessing only the iron-sulfur complex as the prosthetic group. These proteins participate in all major pathways of electron transport: photosynthesis, respiration, hydroxylation and bacterial hydrogen and nitrogen fixation.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Two-ring crystalline hydrocarbons isolated from coal tar. They are used as intermediates in chemical synthesis, as insect repellents, fungicides, lubricants, preservatives, and, formerly, as topical antiseptics.
Estrone derivatives substituted with one or more hydroxyl groups in any position. They are important metabolites of estrone and other estrogens.
A metabolite of tryptophan with a possible role in neurodegenerative disorders. Elevated CSF levels of quinolinic acid are correlated with the severity of neuropsychological deficits in patients who have AIDS.
The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
A species of gram-negative bacteria in the genus PSEUDOMONAS, containing multiple genomovars. It is distinguishable from other pseudomonad species by its ability to use MALTOSE and STARCH as sole carbon and energy sources. It can degrade ENVIRONMENTAL POLLUTANTS and has been used as a model organism to study denitrification.
A species of gram-negative rod-shaped bacteria found ubiquitously and formerly called Comamonas acidovorans and Pseudomonas acidovorans. It is the type species of the genus DELFTIA.
A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.
Phenols substituted with one or more chlorine atoms in any position.
A species of gram-negative, aerobic bacteria found in soil and water. Although considered to be normally nonpathogenic, this bacterium is a causative agent of nosocomial infections, particularly in debilitated individuals.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
A deaminated metabolite of LEVODOPA.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
A species of BURKHOLDERIA considered to be an opportunistic human pathogen. It has been associated with various types of infections of nosocomial origin.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
Any member of the class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting molecules, e.g., ESTERASES, glycosidases (GLYCOSIDE HYDROLASES), lipases, NUCLEOTIDASES, peptidases (PEPTIDE HYDROLASES), and phosphatases (PHOSPHORIC MONOESTER HYDROLASES). EC 3.
A monooxygenase that catalyzes the conversion of BETA-CAROTENE into two molecules of RETINAL. It was formerly characterized as EC 1.13.11.21 and EC 1.18.3.1.
Stable oxygen atoms that have the same atomic number as the element oxygen, but differ in atomic weight. O-17 and 18 are stable oxygen isotopes.
Compounds which restore enzymatic activity by removing an inhibitory group bound to the reactive site of the enzyme.
A group of disorders which have in common elevations of tyrosine in the blood and urine secondary to an enzyme deficiency. Type I tyrosinemia features episodic weakness, self-mutilation, hepatic necrosis, renal tubular injury, and seizures and is caused by a deficiency of the enzyme fumarylacetoacetase. Type II tyrosinemia features INTELLECTUAL DISABILITY, painful corneal ulcers, and keratoses of the palms and plantar surfaces and is caused by a deficiency of the enzyme TYROSINE TRANSAMINASE. Type III tyrosinemia features INTELLECTUAL DISABILITY and is caused by a deficiency of the enzyme 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE. (Menkes, Textbook of Child Neurology, 5th ed, pp42-3)
Naphthalene derivatives carrying one or more hydroxyl (-OH) groups at any ring position. They are often used in dyes and pigments, as antioxidants for rubber, fats, and oils, as insecticides, in pharmaceuticals, and in numerous other applications.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Proteins prepared by recombinant DNA technology.
The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.
An herbicide with irritant effects on the eye and the gastrointestinal system.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.
A highly volatile inhalation anesthetic used mainly in short surgical procedures where light anesthesia with good analgesia is required. It is also used as an industrial solvent. Prolonged exposure to high concentrations of the vapor can lead to cardiotoxicity and neurological impairment.
A species of gram-negative bacteria in the genus PSEUDOMONAS. All strains can utilize FRUCTOSE for energy. It is occasionally isolated from humans and some strains are pathogenic to WATERMELON.
Low-molecular-weight compounds produced by microorganisms that aid in the transport and sequestration of ferric iron. (The Encyclopedia of Molecular Biology, 1994)
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Hydrocarbon rings which contain two ketone moieties in any position. They can be substituted in any position except at the ketone groups.
A large group of aerobic bacteria which show up as pink (negative) when treated by the gram-staining method. This is because the cell walls of gram-negative bacteria are low in peptidoglycan and thus have low affinity for violet stain and high affinity for the pink dye safranine.
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
An enzyme of the oxidoreductase class that catalyzes the reaction between L-tyrosine, L-dopa, and oxygen to yield L-dopa, dopaquinone, and water. It is a copper protein that acts also on catechols, catalyzing some of the same reactions as CATECHOL OXIDASE. EC 1.14.18.1.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
A class of enzymes that catalyze geometric or structural changes within a molecule to form a single product. The reactions do not involve a net change in the concentrations of compounds other than the substrate and the product.(from Dorland, 28th ed) EC 5.
A colorless or slightly yellow crystalline compound obtained from nutgalls. It is used in photography, pharmaceuticals, and as an analytical reagent.
A colorimetric reagent for iron, manganese, titanium, molybdenum, and complexes of zirconium. (From Merck Index, 11th ed)
A species of gram-negative bacteria in the genus PSEUDOMONAS, which is found in SOIL and WATER.
Substances which pollute the soil. Use for soil pollutants in general or for which there is no specific heading.
Compounds that contain a BENZENE ring fused to a furan ring.
An NADPH-dependent flavin monooxygenase that plays a key role in the catabolism of TRYPTOPHAN by catalyzing the HYDROXYLATION of KYNURENINE to 3-hydroxykynurenine. It was formerly characterized as EC 1.14.1.2 and EC 1.99.1.5.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
Proteins, usually acting in oxidation-reduction reactions, containing iron but no porphyrin groups. (Lehninger, Principles of Biochemistry, 1993, pG-10)
Pigment obtained by the oxidation of epinephrine.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.
The relationships of groups of organisms as reflected by their genetic makeup.
Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A methylated metabolite of norepinephrine that is excreted in the urine and found in certain tissues. It is a marker for tumors.
Enzymes that catalyze the addition of a carboxyl group to a compound (carboxylases) or the removal of a carboxyl group from a compound (decarboxylases). EC 4.1.1.
A family of gram-negative aerobic bacteria in the class BETA PROTEOBACTERIA, encompassing the acidovorans rRNA complex. Some species are pathogenic for PLANTS.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A conditionally essential nutrient, important during mammalian development. It is present in milk but is isolated mostly from ox bile and strongly conjugates bile acids.
Extradiol Cleavage of 3-Methylcatechol by Catechol 1,2-Dioxygenase from Various Microorganisms. C. T. Hou, R. Patel and M. O. ... The isofunctional enzymes of catechol 1,2-dioxygenase from species of Acinetobacter, Pseudomonas, Nocardia, Alcaligenes and ... The enzyme 1,2-dihydroxy-6-methylcyclohexa-3,5-dienecarboxylate dehydrogenase uses 1,2-dihydroxy-6-methylcyclohexa-3,5- ... Higson FK, Focht DD (1992). "Degradation of 2-methylbenzoic acid by Pseudomonas cepacia MB2". Appl. Environ. Microbiol. 58 (1 ...
... may refer to: catechol dioxygenase Catechol 1,2-dioxygenase Catechol 2,3-dioxygenase This disambiguation page lists ...
... dioxygenases MeSH D08.811.682.690.416.277 - catechol 1,2-dioxygenase MeSH D08.811.682.690.416.305 - catechol 2,3-dioxygenase ... 4-dioxygenase MeSH D08.811.682.690.416.330 - 4-hydroxyphenylpyruvate dioxygenase MeSH D08.811.682.690.416.333 - indoleamine- ... 4-dioxygenase MeSH D08.811.682.690.416.722 - tryptophan oxygenase MeSH D08.811.682.690.562 - inositol oxygenase MeSH D08.811. ... catechol oxidase MeSH D08.811.682.690.708.125.500 - monophenol monooxygenase MeSH D08.811.682.690.708.170 - cytochrome p-450 ...
4-dihydroxybenzoate to produce catechol and CO2. The enzyme protocatechuate 3,4-dioxygenase uses 3,4-dihydroxybenzoate and O2 ... 4-dioxygenase vanillate monooxygenase 3,4-dihydroxyphthalate decarboxylase 4,5-dihydroxyphthalate decarboxylase Degradation ... 23 (2): 201-208. doi:10.1016/j.tiv.2008.11.008. PMID 19095056. Anter, J.; Romero Jiménez, M.; Fernández Bedmar, Z.; Villatoro ... 1 (7): 232. doi:10.31989/ffhd.v1i7.127. ISSN 2160-3855. Pietta, P. G.; Simonetti, P.; Gardana, C.; Brusamolino, A.; Morazzoni, ...
3-dioxygenase and 4-hydroxyphenylpyruvate dioxygenase all resemble glyoxalase I in structure. Finally, many proteins of unknown ... catechol 2,3-dioxygenase, 3,4-dihydroxyphenylacetate 2, ... 1: 100011. doi:10.1016/j.rechem.2019.100011. Rose IA (July 1957 ... 3-demethylubiquinone-9 3-O-methyltransferase and numerous dioxygenases such as biphenyl-2,3-diol 1,2-dioxygenase, ... 314 ( Pt 2) (2): 463-7. doi:10.1042/bj3140463. PMC 1217073. PMID 8670058. Saint-Jean AP, Phillips KR, Creighton DJ, Stone MJ ( ...
4-dicarboxylate dehydrogenase into a catechol intermediate. The catechol ring is then cleaved by PCA 3,4-dioxygenase before the ... 118 (1): 21-27. doi:10.1099/00221287-118-1-21. ISSN 1350-0872. Coghlan, Andy. "Bacteria found to eat PET plastics could help do ... 25 (1): 53-64. doi:10.1016/j.ejpe.2015.03.001. Carrington, Damian (28 September 2020). "New super-enzyme eats plastic bottles ... Ideonella sakaiensis PET surface and use a secreted PET hydrolase, or PETase, to degrade the PET into mono(2-hydroxyethyl) ...
Iron-containing dioxygenase enzymes catalyze the cleavage of catechol. Catechol is produced by a reversible two-electron, two- ... catechol was listed as pyrocatechol. In 1879, the Journal of the Chemical Society recommended that catechol be called "catechol ... Catechol occurs as feathery white crystals that are very rapidly soluble in water. Catechol was first isolated in 1839 by Edgar ... Catechols produce quinones with the addition of ceric ammonium nitrate (CAN). Catechol is the conjugate acid of a chelating ...
... dependent dioxygenases. The catechol dioxygenases, some of the most well-studied dioxygenase enzymes, use dioxygen to cleave a ... Two important groups of mononuclear, non-heme iron dioxygenases are catechol dioxygenases and 2-oxoglutarate (2OG)- ... Catechol dioxygenases are further classified as being "extradiol" or "intradiol," and this distinction is based on mechanistic ... In this respect, these enzymes are reminiscent of the intradiol catechol dioxygenases whereby the metal centers activate the ...
3-dioxygenase (EC 1.13.11.2), and protocatechuate 3,4-dioxygenase (EC 1.13.11.3). The active site of catechol dioxygenases most ... Catechol dioxygenases are metalloprotein enzymes that carry out the oxidative cleavage of catechols. This class of enzymes ... 3-dioxygenase, is often used as a reporter to quantitate gene expression. An example of the reaction carried out by catechol 1, ... Catechol dioxygenases belong to the class of oxidoreductases and have several different substrate specificities, including ...
Muller R, Schmitt S, Lingens F (1982). "A novel non-heme iron-containing dioxygenase. Chloridazon-catechol dioxygenase from ... Chloridazon-catechol dioxygenase (EC 1.13.11.36) is an enzyme that catalyzes the chemical reaction 5-amino-4-chloro-2-(2,3- ... Muller R, Haug S, Eberspacher J, Lingens F (1977). "[Catechol 2,3-dioxygenase from pyrazon-degrading bacteria (author's transl ... The systematic name of this enzyme class is 5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone 1,2-oxidoreductase ( ...
Pascal RA, Huang DS (July 1986). "Reactions of 3-ethylcatechol and 3-(methylthio)catechol with catechol dioxygenases". Archives ... a family of catechol dioxygenases that cleaves the bond between the phenolic hydroxyl groups of catechol using an Fe3+ cofactor ... Two families of dioxygenases were discovered by Osamu Hayaishi and Kizo Hashimoto in 1950: catechol 1,2-dioxygenase and ... catechol dioxygenase, pyrocatechase, pyrocatechol 1,2-dioxygenase, CD I, CD II) is an enzyme that catalyzes the oxidative ring ...
3-dioxygenase) is an enzyme with systematic name catechol:oxygen 2,3-oxidoreductase (decyclizing). This enzyme catalyses the ... 3-dioxygenase and other dioxygenases (EC 1.13.11.2 and EC 1.14.12.-) in the degradative pathways of 2-aminobenzenesulphonic, ... Catechol+2,3-dioxygenase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal. ... Catechol 2,3-dioxygenase (EC 1.13.11.2, 2,3-pyrocatechase, catechol 2,3-oxygenase, catechol oxygenase, metapyrocatechase, ...
I Enzymatic formation of catechol from benzene". Biochemistry. 7 (7): 2653-62. doi:10.1021/bi00847a031. PMID 4298226. Biology ... and benzene dioxygenase. This enzyme participates in naphthalene and anthracene degradation. It has 4 cofactors: FAD, Iron, ... In enzymology, a benzene 1,2-dioxygenase (EC 1.14.12.3) is an enzyme that catalyzes the chemical reaction benzene + NADH + H+ ... The systematic name of this enzyme class is benzene,NADH:oxygen oxidoreductase (1,2-hydroxylating). Other names in common use ...
... catechol + chloride + NAD+ + CO2 The 4 substrates of this enzyme are 2-chlorobenzoate, NADH, H+, and O2, whereas its 4 products ... are catechol, chloride, NAD+, and CO2. This enzyme belongs to the family of oxidoreductases, specifically those acting on ... In enzymology, a 2-chlorobenzoate 1,2-dioxygenase (EC 1.14.12.13) is an enzyme that catalyzes the chemical reaction 2- ... Fetzner S, Muller R, Lingens F (1989). "Degradation of 2-chlorobenzoate by Pseudomonas cepacia 2CBS". Biol. Chem. Hoppe-Seyler ...
Kobayashi S, Hayaishi O (1970). "Anthranilic acid conversion to catechol (Pseudomonas)". Methods Enzymol. 17A: 505-510. doi: ... catechol + CO2 + NAD(P)+ + NH3 The 5 substrates of this enzyme are anthranilate, NADH, NADPH, H+, and O2, whereas its 5 ... "Enzymatic formation of catechol from anthranilic acid". J. Biol. Chem. 239: 2204-2211. PMID 14209949. Biology portal v t e. ... products are catechol, CO2, NAD+, NADP+, and NH3. This enzyme belongs to the family of oxidoreductases, specifically those ...
Junca, H.; Plumeier, I.; Hecht, H. J. R.; Pieper, D. H. (December 2004). "Difference in kinetic behaviour of catechol 2,3- ... dioxygenase variants from a polluted environment". Microbiology. 150 (12): 4181-4187. doi:10.1099/mic.0.27451-0. PMID 15583170 ... 227 (1-2): 21-30. doi:10.1016/j.mce.2004.08.001. PMID 15501581. Görg, A.; Weiss, W.; Dunn, M. J. (December 2004). "Current two- ... 3.0.CO;2-C. PMID 11678039. Archived from the original (PDF) on 2010-04-01. "Archived copy". Archived from the original on 2011- ...
... related with the catechol and resorcinol groups and the oxidation is pH-dependent. The oxidation of the catechol 3',4'- ... Protocatechuic acid and hydroxyquinol undergo intradiol cleavage through protocatechuate 3,4-dioxygenase and hydroxyquinol 1,2- ... sulfation and methylation of the catechol group by catechol-O-methyl transferase, with only small amounts detected in plasma. ... dioxygenase to form β-carboxy cis, cis-muconic acid and maleyl acetate. Among fungi, degradation of catechin can be achieved by ...
other dioxygenase: Catechol dioxygenase. *Homogentisate 1,2-dioxygenase. *Cysteine dioxygenase. *4-Hydroxyphenylpyruvate ... dioxygenase at the US National Library of Medicine Medical Subject Headings (MeSH) ... naphthalene-1,2-diol + O2 ⇌. {\displaystyle \rightleftharpoons }. 2-hydroxy-2H-chromene-2-carboxylate. This enzyme is involved ... 1,2-dihydroxynaphthalene dioxygenase (EC 1.13.11.56, 1,2-DHN dioxygenase, DHNDO, 1,2-dihydroxynaphthalene oxygenase, 1,2- ...
"The genes coding for the conversion of carbazole to catechol are flanked by IS6100 elements in Sphingomonas sp. strain XLDN2-5 ... 9a-dioxygenase, a three-component dioxygenase system of Pseudomonas resinovorans strain CA10". Applied and Environmental ... 9a-dioxygenase (EC 1.14.12.22, CARDO) is an enzyme with systematic name 9H-carbazole,NAD(P)H:oxygen oxidoreductase (2,3- ... 9a-dioxygenase catalyses the first reaction in the pathway of carbazole degradation. Nam JW, Nojiri H, Noguchi H, Uchimura H, ...
other dioxygenase: Catechol dioxygenase. *Homogentisate 1,2-dioxygenase. *Cysteine dioxygenase. *4-Hydroxyphenylpyruvate ... 114 (1): 78-82. doi:10.1111/bcpt.12115. PMID 24020397.. *^ a b c d e f g Anwar Y, Sabir JS, Qureshi MI, Saini KS (2014). "5- ... ALOX5's dioxygenase activity adds a hydroperoxyl (i.e. HO2) residue to arachidonic acid (i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic ... dioxygenase activity. • metal ion binding. • protein binding. • oxidoreductase activity. • oxidoreductase activity, acting on ...
Next, the diol is newly reduced by NADH to catechol. The catechol is then metabolized to acetyl CoA and succinyl CoA, used by ... In bacteria, dioxygenase enzyme can add an oxygen to the ring, and the unstable product is immediately reduced (by NADH) to a ... and hydroquinone to both benzenetriol and catechol. Hydroquinone, benzenetriol and catechol are converted to polyphenols. In ... catechol, hydroquinone and 1,2,4-trihydroxybenzene. Most of these metabolites have some value as biomarkers of human exposure, ...
Catechol oxidase EC 1.10.3.1 Laccase EC 1.10.3.2 Category:EC 1.10.99 (with other acceptors) Category:EC 1.11.1 (peroxidases) ... 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) Category:EC 1.13.12 (With incorporation of one atom of oxygen (internal ... EC 1.14.12 Nitric oxide dioxygenase Category:EC 1.14.13 Nitric oxide synthase EC 1.14.13.39 Category:EC 1.14.14 Aromatase EC ... List of EC numbers (EC 5) List of EC numbers (EC 6) Dehydrogenase Luciferase DMSO reductase Category:EC 1.1.1 (with NAD+ or ...
Catechol dioxygenases. Department of Chemistry, Michigan State University, East Lansing, MI 488241322, U.S.A. ... Diversity of catechol 2,3-dioxygenase genes of bacteria responding to dissolved organic matter derived from different sources ... PCR isolation of catechol 2,3-dioxygenase gene fragments from environmental samples and their assembly into functional genes. ... The Catechol 1,2 Dioxygenase System of Acinetobacter radioresistens: Isoenzymes, Inductors and Gene localisation. Biol. Chem., ...
Junca, H.; Plumeier, I.; Hecht, H. J. R.; Pieper, D. H. (December 2004). "Difference in kinetic behaviour of catechol 2,3- ... dioxygenase variants from a polluted environment". Microbiology. 150 (12): 4181-4187. doi:10.1099/mic.0.27451-0. PMID 15583170. ... 1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] and a crucial step in gene expression profiling. ... 6 (1): 172-180. doi:10.1002/pmic.200500086. PMID 16317771.. *^ Stasyk, T.; Morandell, S.; Bakry, R.; Feuerstein, I.; Huck, C. W ...
Insight into the Mechanism of the Extradiol Catechol Dioxygenases". Journal of the American Chemical Society. 123 (21): 5030- ... Lin, Gang; Reid, Gillian; Bugg, Timothy D. H. (May 2001). "Extradiol Oxidative Cleavage of Catechols by Ferrous and Ferric ... 2 (4): 420-426. doi:10.1039/C5MH00030K. "Novel thin film approach for memory and energy harvesting devices". Printed ... Groups 1-6) with thioether, selenoether and telluroether ligands". Dalton Transactions. 45 (46): 18393-18416. doi:10.1039/ ...
... chloridazon-catechol dioxygenase EC 1.13.11.37: hydroxyquinol 1,2-dioxygenase EC 1.13.11.38: 1-hydroxy-2-naphthoate 1,2- ... 3-dioxygenase EC 1.13.11.18: sulfur dioxygenase EC 1.13.11.19: cysteamine dioxygenase EC 1.13.11.20: cysteine dioxygenase EC ... gibberellin 3b-dioxygenase EC 1.14.11.16: peptide-aspartate b-dioxygenase EC 1.14.11.17: taurine dioxygenase EC 1.14.11.18: ... 5-dioxygenase EC 1.14.12.8: 4-sulfobenzoate 3,4-dioxygenase EC 1.14.12.9: 4-chlorophenylacetate 3,4-dioxygenase EC 1.14.12.10: ...
For example, Rhodococci expresses dioxygenases, which can be used to degrade benzotrifluoride, a recalcitrant pollutant. ... catechol, benzoate, and protocatechuic acid. Rhodococci are also capable of accumulating heavy metal ions, such as radioactive ... "Degradation of benzotrifluoride via the dioxygenase pathway in Rhodococcus sp. 065240". Bioscience, Biotechnology, and ... ISBN 978-1-904455-30-1. [1]. McLeod MP, Warren RL, Hsiao WW, Araki N, Mihre M, Fernandes C, Miyazawa D, Wong W, Lillquist AL, ...
Caffeate 3,4-dioxygenase is an enzyme that uses caffeic acid and oxygen to produce 3-(2-carboxyethenyl)-cis,cis-muconate. 3-O- ... The developing chemistry is similar to that of catechol or pyrogallol. It is also used as a matrix in MALDI mass spectrometry ... 4-methoxyphenol and catechol at low doses, either alone or in combination, and modulation of their effects in a rat medium-term ... 131 (1): 66-71. doi:10.1093/jn/131.1.66. PMID 11208940. "EC 4.3.1.11". www.chem.qmul.ac.uk. Archived from the original on 3 ...
Heating catechin past its point of decomposition releases pyrocatechol (also called catechol), which explains the common origin ... leucoanthocyanidin dioxygenase), E11, anthocyanidin reductase. HSCoA, Coenzyme A. L-Tyr, L-tyrosine, L-Phe, L-phenylalanine. ... 277 (2): 507-512. doi:10.1006/bbrc.2000.3701. ISSN 0006-291X. PMID 11032751. Das, N. P. (December 1971). "Studies on flavonoid ... Figure 1:Schematic overview of the flavan-3-ol (-)-epicatechin biosynthesis in plants: Enzymes are indicated in blue, ...
4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27). *Category:EC 1.13.12 (With incorporation of one atom of oxygen (internal ... Catechol-O-methyl transferase EC 2.1.1.6. *DNA methyltransferase EC 2.1.1.72, EC 2.1.1.113, EC 2.1.1.37 ... Category:EC 1.10.1(with NAD+ or NADP+ as acceptor). *Category:EC 1.10.2 (with a cytochrome as acceptor) *Coenzyme Q - ... Category:EC 1.3.2 (with a cytochrome as acceptor). *Category:EC 1.3.3 (with oxygen as acceptor) *Protoporphyrinogen oxidase EC ...
The meta-hydroxyl group of catechol is methylated by catechol-O-methyltransferase. Four of the five hydroxyl groups of ... 3-dioxygenase. *Quercetin 3-O-methyltransferase. *Quercetin-3-sulfate 3'-sulfotransferase ... 2 (5): 235-44. doi:10.1039/c1fo10025d. PMC 4122511. PMID 21779561.. *^ a b Day AJ, Rothwell JA, Morgan R (2004). " ... Gross P (March 1, 2009), New Roles for Polyphenols. A 3-Part Report on Current Regulations & the State of Science, ...
Oxidoreductases: dioxygenases, including steroid hydroxylases (EC 1.14). 1.14.11: 2-oxoglutarate. *Prolyl hydroxylase ... 10 (6 Pt 2): 774-81. doi:10.1159/000073965. PMID 14631117.. *^ a b Halskau Ø, Ying M, Baumann A, Kleppe R, Rodriguez-Larrea D, ... 267 (1): 456-61. doi:10.1006/bbrc.1999.1945. PMID 10623641.. *^ Ono M, Okamoto M, Kawabe N, Umezawa H, Takeuchi T (Mar 1971). " ... 42 (1-2): 29-32. doi:10.1159/000132246. PMID 2872999.. *. Grima B, Lamouroux A, Boni C, Julien JF, Javoy-Agid F, Mallet J (1987 ...
... can be directly metabolized by catechol-O-methyl transferase to 3-O-methyldopa, and then further to vanillactic acid. ... 4-Hydroxyphenylpyruvate dioxygenase. *Homogentisate 1,2-dioxygenase. *Fumarylacetoacetate hydrolase. *tyrosine→melanin: ... 171 (2): 403-14. doi:10.1111/bph.12459.. *^ Jürgen Martens, Kurt Günther, Maren Schickedanz: "Resolution of Optical Isomers by ... 2 (3): 126-133.. *^ Waite, J. Herbert; Andersen, Niels Holten; et al. (2005). "Mussel Adhesion: Finding the Tricks Worth ...
Pascal RA, Huang DS (July 1986). "Reactions of 3-ethylcatechol and 3-(methylthio)catechol with catechol dioxygenases". Archives ... a family of catechol dioxygenases that cleaves the bond between the phenolic hydroxyl groups of catechol using an Fe3+ cofactor ... Two families of dioxygenases were discovered by Osamu Hayaishi and Kizo Hashimoto in 1950: catechol 1,2-dioxygenase and ... catechol dioxygenase, pyrocatechase, pyrocatechol 1,2-dioxygenase, CD I, CD II) is an enzyme that catalyzes the oxidative ring ...
CATECHOL 1,2-DIOXYGENASE. A, B. 311. Acinetobacter baylyi ADP1. Mutation(s): 0 Gene Names: catA, ACIAD1442. EC: 1.13.11.1. ... This is in contrast to the archetypical intradiol dioxygenase protocatechuate 3,4-dioxygenase (3,4-PCD), which forms more ... STRUCTURE OF CATECHOL 1,2-DIOXYGENASE FROM ACINETOBACTER SP. ADP1 INHIBITED BY BOUND MERCURY. *DOI: 10.2210/pdb1DLQ/pdb ... Catechol 1,2-dioxygenases (1, 2-CTDs) have a rudimentary design structure - a homodimer with one catalytic non-heme ferric ion ...
Phenol monooxygenase (PMO) and catechol 1,2-dioxygenase (C12O), the two first enzymes of the phenol-degradation pathways, are ... Sequence of the plasmid-encoded catechol 1,2-dioxygenase-expressing gene, pheB, of phenol-degrading Pseudomonas sp. strain ... 1. Laboratory of Plasmid Biology, Estonian Biocenter, Tartu, U.S.S.R.. ... Kivisaar M1, Kasak L, Nurk A.. Author information. ...
Crystal structure determination of Catechol 1,2-Dioxygenase from Rhodococcus opacus 1CP in complex with 3,5-dichlorocatechol ... ion containing enzyme specialized in the aerobic biodegradation of catechols, and its adducts with catechol, 3-methylcatechol, ... Catechol 1,2-dioxygenase from the Gram-positive Rhodococcus opacus 1CP: Quantitative structure/activity relationship and the ... The structural analyses reveal the diverse modes of binding to the active metal iron ion of the tested catechols thus allowing ...
... catechol dioxygenase was formed in E. coli at twice the level found in fully induced cultures of A. calcoaceticus. A. ... Catechol 1,2-dioxygenase (EC 1.13.1.1), the product of the catA gene, catalyzes the first step in catechol utilization via the ... Cloning and expression of Acinetobacter calcoaceticus catechol 1,2-dioxygenase structural gene catA in Escherichia coli.. E L ... Cloning and expression of Acinetobacter calcoaceticus catechol 1,2-dioxygenase structural gene catA in Escherichia coli. ...
G27 strain catechol 1,2-dioxygenase were determined. The enzyme had apparent Km of 29 µM for catechol and the cleavage ... The catechol 1,2-dioxygenase (C1,2O) was purified using four steps of ammonium sulfate precipitation, DEAE-celullose, Sephadex ... A half-life of the catechol 1,2-dioxygenase at the optimum temperature was 40 min. The kinetic parameters of the Geobacillus sp ... The enzyme was purified about 18-fold with a specific activity of 7.42 U mg of protein−1. The relative molecular mass of the ...
The aim of the study was to purify, characterize and predict template-based three-dimensional structure of catechol 1,2- ... dioxygenase (C12O) from indigenous Pseudomonas chlororaphis strain UFB2 (PcUFB2). Preliminary studies showed that PcUFB2 could ... Catechol dioxygenases in microorganisms cleave catechol into cis-cis-muconic acid or 2-hydroxymuconic semialdehyde via the ... Characterization and optimization of C12O activity can assist in understanding the 2,4-DCP metabolic pathway in PcUFB2 and its ...
other dioxygenase: Catechol dioxygenase. *Homogentisate 1,2-dioxygenase. *Cysteine dioxygenase. *4-Hydroxyphenylpyruvate ... dioxygenase at the US National Library of Medicine Medical Subject Headings (MeSH) ... naphthalene-1,2-diol + O2 ⇌. {\displaystyle \rightleftharpoons }. 2-hydroxy-2H-chromene-2-carboxylate. This enzyme is involved ... 1,2-dihydroxynaphthalene dioxygenase (EC 1.13.11.56, 1,2-DHN dioxygenase, DHNDO, 1,2-dihydroxynaphthalene oxygenase, 1,2- ...
Kobayashi S, Hayaishi O (1970). "Anthranilic acid conversion to catechol (Pseudomonas)". Methods Enzymol. 17A: 505-510. doi: ... catechol + CO2 + NAD(P)+ + NH3 The 5 substrates of this enzyme are anthranilate, NADH, NADPH, H+, and O2, whereas its 5 ... "Enzymatic formation of catechol from anthranilic acid". J. Biol. Chem. 239: 2204-2211. PMID 14209949. Biology portal v t e. ... products are catechol, CO2, NAD+, NADP+, and NH3. This enzyme belongs to the family of oxidoreductases, specifically those ...
Engineering catechol 1, 2-dioxygenase by design for improving the performance of the cis, cis-muconic acid synthetic pathway in ... Rights & permissionsfor article Engineering catechol 1, 2-dioxygenase by design for improving the performance of the ,i,cis, ... LTP-1, a novel antimitotic agent and Stat3 inhibitor, inhibits human pancreatic carcinomas in vitro and in vivo *Han-Li Huang ... 2 September 2016 , Open Rapid and cyclic dust accumulation during MIS 2 in Central Asia inferred from loess OSL dating and ...
DE SubName: Full=Catechol dioxygenase, putative (AFU_orthologue AFUA_2G02910) {ECO:0000313,EMBL:CBF84491.1}; GN ORFNames=ANIA_ ... DR Pfam; PF00775; Dioxygenase_C; 1. DR Pfam; PF04444; Dioxygenase_N; 1. DR SUPFAM; SSF49482; SSF49482; 1. PE 4: Predicted; DR ... DR GO; GO:0018576; F:catechol 1,2-dioxygenase activity; IEA:InterPro. DR GO; GO:0008199; F:ferric iron binding; IEA:InterPro. ... DR GO; GO:0009712; P:catechol-containing compound metabolic process; IEA:InterPro. DR Gene3D; 2.60.130.10; -; 1. DR InterPro; ...
Isolation and partial characterization of catechol 1,2-dioxygenase of phenol degrading yeast Candida tropicalis.. Vilimkova L, ... Isolation and partial characterization of extracellular NADPH-dependent phenol hydroxylase oxidizing phenol to catechol in ... Catechols:chemistry, Chromatography, Chromatography, Gel, Chromatography, High Pressure Liquid, Cytosol:chemistry, Dextrans, ... Catechols:metabolism, Chromatography, High Pressure Liquid, Cloning, Molecular, Comamonas testosteroni:enzymology, ...
Isolation and partial characterization of catechol 1,2-dioxygenase of phenol degrading yeast Candida tropicalis.. Vilimkova L, ... Catechols:chemistry, Chromatography, Chromatography, Gel, Chromatography, High Pressure Liquid, Cytosol:chemistry, Dextrans, ... Isolation and partial characterization of catechol 1,2-dioxygenase of phenol degrading yeast Candida tropicalis. Neuro ... Journal Article 2009; 30(Suppl 1): 72-79 PubMed PMID: 20027148 Keywords: Absorption, Amino Acid Sequence, Animals, Apoproteins: ...
Structure of Catechol 1,2-dioxygenase from Acinetobacter Calcoaceticus Native Data.jpg 500 × 500; 48 KB. ...
... catechol , / 1,2-dioxygenase ,/ 1.13.11.1 , Search GenBank, 769 hits on Apr. 03, 2012 Kyoto ,\ ExPASy , \ , 2H+ v 3-Fluoro-cis, ... Appl Environ Microbiol (1980) 39(1): 58-67. Search Medline for 4-fluorocatechol metabolism AND bacteria. 17 citations on ...
Search Medline for catechol 1,2-dioxygenase AND 4-chlorocatechol. 36 citations on March 09, 2012. 4-Chlorocatechol , , O2 ... catechol , / 1,2-dioxygenase ,/ 1.13.11.1 , Search GenBank, 769 hits on Apr. 03, 2012 Kyoto ,\ ExPASy , \ , 2H+ v 3-Chloro-cis, ... 2,4-Dichlorophenoxyacetic Acid] [BBD Main Menu] Page Author(s): Brian Hill July 03, 2003 Contact Us This is the EAWAG-BBD ...
3-dioxygenase. misc/catechol Reaction: catechol + O2 = 2-hydroxymuconate-6-semialdehyde. For diagram of reaction click here.. ... 3-dioxygenase. EC 1.13.11.18 sulfur dioxygenase. EC 1.13.11.19 cysteamine dioxygenase. EC 1.13.11.20 cysteine dioxygenase. EC ... EC 1.13.11.87 endo-cleaving rubber dioxygenase. EC 1.13.11.1. Accepted name: catechol 1,2-dioxygenase. Reaction: catechol + O2 ... A novel non-heme iron-containing dioxygenase. Chloridazon-catechol dioxygenase from Phenylobacterium immobilis DSM 1986. Eur. J ...
3-dioxygenase, encoded by ntdAaAbAcAd. The catechol degradation (Ctd) enzymes were identified based on the analysis of the JS42 ... catechol 2,3-dioxygenase; CtdF, 2-hydroxymuconate semialdehyde hydrolase; CtdJ, 2-oxopent-4-dienoate hydratase; CtdK, 4-hydroxy ... strain JS42 to 2-nitrotoluene.. Rabinovitch-Deere CA1, Parales RE.. Author information. 1. Department of Microbiology, College ... Fig 2. Wild-type JS42m responses in the qualitative capillary assay. Responses to buffer (negative control) or succinate (10 mM ...
It was shown that in E. coli PCA and catechol can be produced this way, with the highest yield being that of catechol at a ... Thompson, B. (2017). Engineering Escherichia coli for the Novel and Enhanced Biosynthesis of Phenol, Catechol, and Muconic Acid ... It has been proposed that this precursor is rather 4-hydroxyphenylpyruvate then catechol, giving rise to a second pathway to ... and catechol by means of the aroZ gene from Klebsiella pneumoniae encoding DHS dehydratase, the aroY gene from K. pneumoniae ...
strain P51 tcb gene cluster, which encodes metabolism of chlorinated catechols: evidence for specialization of catechol 1,2- ... dioxygenases for chlorinated substrates.. J R van der Meer, R I Eggen, A J Zehnder, W M de Vos ... strain P51 tcb gene cluster, which encodes metabolism of chlorinated catechols: evidence for specialization of catechol 1,2- ... strain P51 tcb gene cluster, which encodes metabolism of chlorinated catechols: evidence for specialization of catechol 1,2- ...
... complexes with a tripodal N3O ligand containing an internal base as a model for catechol intradiol-cleaving dioxygenases. Li, ... protocatechuate 3,4-dioxygenase as an internal base. The spectrop h oto metric titration experiment indicates the relatively ... reactive functional-model, nonheme iron enzymes, protocatechuate 3,4-dioxygenase, crystal-structure, active-sites, (catecholato ... and the coordinate ethoxyl arm of the ligand is capable of accepting the proton from catechol, which mimics the function of ...
Aromatic catabolism Free-living diazotrophs Nitrogen fixation catechol dioxygenases Protocatechuate dioxygenases A preliminary ... All six species growing on substrates typically converted to catechol expressed inducible catechol 1,2-dioxygenase and/or ... 5-dioxygenase was expressed. A. chroococcum expressed only ortho cleavage dioxygenases during growth on naphthalene and 4- ... toluate and only meta cleavage dioxygenases on the other aromatics. B. mobilis expressed only ortho cleavage dioxygenases. The ...
The cells grown on benzoate and catechol showed mainly catechol 1,2- dioxygenase activity. The activity of 2,3-dioxygenase was ... Protocatechuate 3,4-dioxygenase was found in crude cell extracts of this strain after incubation with 4-hydroxybenzoic acid, ... Furthermore, the xylE gene which encodes a catechol 2,3-dioxygenase was amplified by PCR, and in two strains the nahAc gene, a ... Because of broad spectrum of dioxygenases types that Stenotrophomonas maltophilia KB2 can exhibit, this strain appears to be ...
To go further we measured the biodegradation rates of Phenol and Catechol by one of the most active strain (Rhodococcus ... We focused our work on the biodegradation of phenol and catechol in clouds using two strategies. 1) A metatranscriptomic ... We detected transcripts of genes coding for phenol monooxygenases (and phenol hydroxylases) and catechol 1,2-dioxygenases. ... while biodegradation of catechol was ten times quicker than chemical transformation. The experimentally derived biodegradation ...
Rhodococcus rhodochrous catechol 1,2-dioxygenase (catA) gene, partial cds. DSM 43241 T ... Applicability of the functional gene catechol 1,2-dioxygenase as a biomarker in the detection of BTEX-degrading Rhodococcus ... 33 items found, displaying 1 to 25.[First/Prev] 1, 2 [Next/Last] ... 33 items found, displaying 1 to 25.[First/Prev] 1, 2 [Next/Last ... Proc Natl Acad Sci U S A 101(1), 314-319, 2004 ...
A multidisciplinary characterization of catechol 1,2 dioxygenase. Biochimica et Biophysica Acta, 1814(6), 817-823.Google ... Indian Journal of Pharmaceutical Sciences, 74(1), 1-17.PubMedPubMedCentralGoogle Scholar ... Journal of Laboratory Automation, 18(1), 19-29.PubMedPubMedCentralGoogle Scholar ... Expert Review of Proteomics, 8(1), 43-59.PubMedPubMedCentralGoogle Scholar ...
To produce adipic acid in Escherichia coli, we utilize an enzyme cascade of catechol 1,2-dioxygenase (CatA) and enoate ... One common pathway for microbial production of adipic acid goes through a catechol intermediate to form muconic acid, which is ... To quantify metabolism of different substituted catechols, we developed a semi-quantification method based on the conserved ... Furthermore, we characterize our enzyme cascade by analyzing its metabolism of different substituted catechols available from ...
The gentisate pathway appears to be less common than the catechol pathway. The benzene ring of catechol is cleaved by a 1,2- ... Isolation and characterization of the genes encoding a novel oxygenase component of angular dioxygenase from the gram-positive ... Catechol and gentisate are the common intermediates in salicylate degradation, but direct fission (28) and CoA addition have ... Cloning and expression of thermophilic catechol 1,2-dioxygenase gene (catA) from Streptomyces setonii.FEMS Microbiol. Lett.195: ...
Diversity of the ring-cleaving dioxygenase gene pcaH in a salt marsh bacterial community. Appl. Environ. Microbiol. 67:5801- ... DNA sequence of the Acinetobacter calcoaceticus catechol 1,2-dioxygenase I structural gene catA: evidence for evolutionary ... Spontaneous mutations in pcaH and -G, structural genes for protocatechuate 3,4-dioxygenase in Acinetobacter calcoaceticus. J. ... DNA sequences of genes encoding Acinetobacter calcoaceticus protocatechuate 3,4-dioxygenase: evidence indicating shuffling of ...
... catechol(1,2-dihydroxybenzene), resorcinol(1,3-dihydroxybenzene and hydroquinone(1,4-dihydroxybenzene) without preliminary ... 273nm and 288mp respectively.The average recoveries of catechol, resorcinol and hydroquinone in standard mixtures are 97.4%, ... Simultaneous Determination of Catechol, Resorcinol and Hydroquinone with Kalman Filtering Ultraviolet Spectrophotometry. ... Simultaneous determination of catechol and hydroquinone in graphene modified electrode[J];武汉工程大学学报;2013-02. ...
  • Thus far, 1,2-CTD has been observed to exist in the following species of soil bacteria and fungi: Pseudomonas sp. (wikipedia.org)
  • the 1,2-CTD enzyme produced by Pseudomonas arvilla is the exception to this rule, containing two highly homologous subunits that can form either a homo- or hetero- dimer. (wikipedia.org)
  • Sequence of the plasmid-encoded catechol 1,2-dioxygenase-expressing gene, pheB, of phenol-degrading Pseudomonas sp. (nih.gov)
  • Phenol monooxygenase (PMO) and catechol 1,2-dioxygenase (C12O), the two first enzymes of the phenol-degradation pathways, are encoded by a 3.4-kb DNA fragment cloned from Pseudomonas sp. (nih.gov)
  • The aim of the study was to purify, characterize and predict template-based three-dimensional structure of catechol 1,2-dioxygenase (C12O) from indigenous Pseudomonas chlororaphis strain UFB2 ( Pc UFB2). (preprints.org)
  • ES-MS spectra of tryptic digested SDS-PAGE band and bioinformatics studies revealed that C12O share 81% homology to homogentisate 1,2-dioxygenase reported in other Pseudomonas chlororaphis strains. (preprints.org)
  • Fujisawa H (1970) Protocatechuate 3,4-dioxygenase ( Pseudomonas ). (springer.com)
  • Nakazawa T, Yokota T (1973) Benzoate metabolism in Pseudomonas putida (arvilla) mt-2: demonstration of two benzoate pathways. (springer.com)
  • p-Nitrophenol Degradation Characteristics of Hydroxyquinol 1,2-Dioxygenase Gene (pnpC) Knock-out Mutant of Pseudomonas putida DLL-E4. (cnki.com.cn)
  • We have studied the well known oxygenase, catechol 1, 2-dioxygenase, the gene of which has been successfully cloned and we reported for the first time existence of 3 isozymes of this enzyme from a Pseudomonas. (nii.ac.jp)
  • This enables the native organism, Pseudomonas putida, to use catechol as an alternate energy source to a certain extent and may also result in similar capability for our engineered E. coli strain. (igem.org)
  • Catechol biosynthesis from glucose in Escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1 Microbial Cell Factories, 13, 136. (unam.mx)
  • Pseudomonas putida mt-2 harbors two different routes for catabolism of catechol, namely one meta pathway encoded by the xyl genes of the TOL plasmid pWW0 and one ortho pathway determined by the chromosomal ben and cat genes. (csic.es)
  • Catechol 2,3-dioxygenase (xylE) is found in Pseudomonas putida and hydrolyzes catechol into 2-HMS, which can enter the citric acid cycle 6 . (igem.org)
  • Bacterial metabolism of side chain fluorinated aromatics: cometabolism of 3-trifluoromethyl(TFM)-benzoate by Pseudomonas putida (arvilla) mt-2 and Rhodococcus rubropertinctus N657. (cbrc.jp)
  • Crystallization of catechol-1,2 dioxygenase from Pseudomonas arvilla C-1. (cbrc.jp)
  • Quantitative structure/activity relationship for the rate of conversion of C4-substituted catechols by catechol-1,2-dioxygenase from Pseudomonas putida (arvilla) C1. (cbrc.jp)
  • This reaction has been observed in several subspecies of Pseudomonas putida, in Burkholderia cepacia, Sphingomonas and Bacillus stearothermophilus, in activated sludge microorganisms [A2397, E31, J654, K5], as well as being catalysed by chlorocatechol 2,3-dioxygenase and 1,2-dihydroxynaphthalene oxygenase (see below). (ormedmedical.us)
  • Recombinant CatA (catechol 1,2-dioxygenase, C12O) from the organism was active over a broad temperature range (5-37°C). However, CatA rapidly lost the enzyme activity when incubated at above 25°C. For example, 1h-preincubation at 37°C resulted in 100% loss of enzyme activity, while a counterpart from mesophilic Pseudomonas putida mt-2 did not show any negative effect on the initial enzyme activity. (elsevier.com)
  • Nucleotide sequence and expression of the catechol 2,3-dioxygenase-encoding gene of phenol-catabolizing Pseudomonas CF600. (diva-portal.org)
  • When expressed in Escherichia coli, the recombinant AntABC (anthranilate 1,2-dioxygenase, AntDO) converted anthranilate into catechol, exhibiting strict specificity toward anthranilate. (elsevier.com)
  • Moreover the high structural homology of the present enzyme with the 3-chlorocatechol 1,2-dioxygenase from the same bacterium are discussed in terms of their different substrate specificity. (rcsb.org)
  • The enzyme was purified about 18-fold with a specific activity of 7.42 U mg of protein−1. (semanticscholar.org)
  • 1,2-dihydroxynaphthalene dioxygenase ( EC 1.13.11.56 , 1,2-DHN dioxygenase , DHNDO , 1,2-dihydroxynaphthalene oxygenase , 1,2-dihydroxynaphthalene:oxygen oxidoreductase ) is an enzyme with systematic name naphthalene-1,2-diol:oxygen oxidoreductase . (wikipedia.org)
  • The systematic name of this enzyme class is anthranilate,NAD(P)H:oxygen oxidoreductase (1,2-hydroxylating, deaminating, decarboxylating). (wikipedia.org)
  • To produce adipic acid in Escherichia coli, we utilize an enzyme cascade of catechol 1,2-dioxygenase (CatA) and enoate reductase (ER). (aiche.org)
  • Furthermore, we characterize our enzyme cascade by analyzing its metabolism of different substituted catechols available from lignin pyrolysis. (aiche.org)
  • This method allowed us to quantify compounds via liquid chromatography-mass spectroscopy that do not have commercially available standards, and compare conversion of substituted catechols by our adipic acid-producing enzyme cascade. (aiche.org)
  • The Mn sites mimic the manganese-dependent dioxygenase (MndD), which is an enzyme that catalyses the oxidation of catechol derivatives. (rsc.org)
  • Lactone synthesis activity in a site-directed mutant of an extradiol catechol dioxygenase enzyme. (warwick.ac.uk)
  • For the elucidation of the active site structure of another well characterized oxygenase, catechol 2, 3 - dioxygense, we used o-nitrophenol, a competitive inhibitor, and found it a useful active site probe for this enzyme. (nii.ac.jp)
  • The structural and spectroscopic characterization of mononuclear iron(III)-catecholato complexes of ligand L4 (methyl bis(1-methylimidazol-2-yl)(2-hydroxyphenyl)methyl ether, HL4) are described, which closely mimic the enzyme-substrate complex of the intradiol-cleaving catechol dioxygenases. (uu.nl)
  • Protocatechuate 3,4-dioxygenase (EC 1.13.11.3 ) (3,4-PCD), an oligomeric enzyme complex which consists of 12 copies each of an α and a β subunits. (expasy.org)
  • The enzyme activity was followed by HPLC (catechol consumption and/or cis,cis-muconic acid formation). (cuni.cz)
  • The catechol 2,3-dioxygenase (C23O) enzyme of this pathway catalyses the conversion of catechol to 2-hydroxymuconic semialdehyde. (diva-portal.org)
  • The genome sequence of R.pyridinivorans strain AK37 has identified several key enzymes involved in the six pathways of monocyclic aromatic compound biodegradation: protocatechuate 3,4-dioxygenase, benzoate 1,2-dioxygenase, 3-ketosteroid-9α-hydroxylase, 3-ketosteroid-σ-dehydrogenase, and so on. (kenyon.edu)
  • Biodegradation has long been seen as a cost-effective and ecological way to eliminate environmental contamination [ 2 ]. (hindawi.com)
  • The GC-MS data also shows the presence of catechol and 2-hydroxymuconic semialdehyde , which confirms the established pathway of benzene biodegradation. (thefreedictionary.com)
  • International Journal of Environmental Bioremediation & Biodegradation , 2014 2 (2), pp 75-83. (sciepub.com)
  • Catechol 1,2-dioxygenase (EC 1.13.1.1), the product of the catA gene, catalyzes the first step in catechol utilization via the beta-ketoadipate pathway. (asm.org)
  • Catechol dioxygenases in microorganisms cleave catechol into cis - cis -muconic acid or 2-hydroxymuconic semialdehyde via the ortho- or meta- pathway, respectively. (preprints.org)
  • Characterization and optimization of C12O activity can assist in understanding the 2,4-DCP metabolic pathway in Pc UFB2 and its possible application in bioremediation strategies. (preprints.org)
  • The occurrence of 2- to 3-bp deletions required the stationary-phase sigma factor RpoS, which indicates that some mutagenic pathway is positively controlled by RpoS in P. putida . (asm.org)
  • 2-Nitrotoluene (2NT) degradation pathway in Acidovorax sp. (nih.gov)
  • One common pathway for microbial production of adipic acid goes through a catechol intermediate to form muconic acid, which is then reduced to form adipic acid. (aiche.org)
  • The gentisate pathway appears to be less common than the catechol pathway. (asm.org)
  • THe pathway of aromatic compound degradation by strain AK 37 has not been described ( 1 ). (kenyon.edu)
  • A previously developed genome-scale reconstruction of E. coli (iJR904) was altered to include a catechol transport reaction and a reaction representing the breakdown of catechol via the pathway specified in our design. (igem.org)
  • The catechol pathway that is inserted into E. coli is shown below. (igem.org)
  • The inserted degradation pathway breaks down catechol into acetyl-CoA, which plays a central role in biochemical networks by serving as a connecting hub for many metabolic pathways. (igem.org)
  • The lower pathway includes cleavage of the aromatic ring by dioxygenases with formation of (chloro)maleylacetates. (asmscience.org)
  • To measure the effect of our pathway manipulations on the viability of E.coli exposed to catechol we plan to perform a tolerance test. (igem.org)
  • In order to determine which enzymes would most benefit from metabolic channeling in the catechol degradation pathway, we used a cellular simulation tool, developed in our lab, called Cell++. (igem.org)
  • 2006). We gathered kinetic data of the five enzymes in the degradation pathway and investigated the effects of localizing pairs of sequential enzymes (i.e. catechol 1, 2-dioxygenase and muconate cycloisomeras) on metabolite concentrations. (igem.org)
  • As shown in Fig. ​ Fig.1A, 1A , strain TFA metabolizes tetralin through a meta -cleavage pathway that involves an initial dioxygenation step (catalyzed by the enzymatic complex ThnA1A2A3A4), followed by a dehydrogenation step (catalyzed by ThnB) to produce 1,2-dihydroxytetralin ( 24 ). (pubmedcentralcanada.ca)
  • Instead, the strain contained catechol 1,2-dioxygenase and the alpha/beta subunits of protocatechuate 3,4-dioxygenase, indicating use of the β-ketoadipate pathway to degrade PHE and related aromatic compounds. (lancs.ac.uk)
  • Addition of bipyridyl to incubated medium resulted in accumulation of terminal aromatic compound, suggesting that catechol may be terminal aromatic compound in degradation pathway of H-acid by A. latus. (iisc.ernet.in)
  • The data consistently indicated that induction of the ortho pathway by benzoate plasmid-less strain P. putida KT2440 led to catechol surplus, the toxicity of which at high concentrations being counteracted by CatA2. (csic.es)
  • The cells grown on benzoate and catechol showed mainly catechol 1,2- dioxygenase activity. (bireme.br)
  • Cloning and expression of Acinetobacter calcoaceticus catechol 1,2-dioxygenase structural gene catA in Escherichia coli. (asm.org)
  • When the catA-containing fragment was placed under the control of the lac promoter on pUC19 and induced with isopropylthiogalactopyranoside, catechol dioxygenase was formed in E. coli at twice the level found in fully induced cultures of A. calcoaceticus. (asm.org)
  • P. putida mt-2 has a second chromosomal copy of the catA gene (named catA2) located downstream of the ben operon that encodes an additional catechol-1,2-dioxygenase. (csic.es)
  • Catechol 1,2-dioxygenase (EC 1.13.11.1 ) (gene catA or clcA). (expasy.org)
  • Purification and characterization of catechol 1,2-dioxygenase from Rhodococcus rhodochrous NCIMB 13259 and cloning and sequencing of its catA gene. (cbrc.jp)
  • The presence of Catechol-1,2-dioxygenase (C12D) or Catechol-2,3-dioxygenase (C23D) was detected by the formation of products cis-cis muconic acid and 2-hydroxymuconic semialdehyde (2-HMS) at 260 nm and 375 nm, respectively (Santos and Linardi, 2004). (thefreedictionary.com)
  • The toxic organic compounds can be broken down into molecules that are metabolizable by all organisms, such as 2-hydroxymuconic semialdehyde (2-HMS) 3 . (igem.org)
  • The product is 2,4-dihydroxy-5-methyl-cis, cis -muconic semialdehyde. (ormedmedical.us)
  • The structural analyses reveal the diverse modes of binding to the active metal iron ion of the tested catechols thus allowing to identify the residues selectively involved in recognition of the diverse substrates by this class of enzymes. (rcsb.org)
  • The catalytic rates for Rho 1,2-CTD conversion of the tested compounds are also compared with the calculated energies of the highest occupied molecular orbital (E(HOMO)) of the substrates. (rcsb.org)
  • All six species growing on substrates typically converted to catechol expressed inducible catechol 1,2-dioxygenase and/or catechol 2,3-dioxygenase. (springer.com)
  • When grown on substrates typically converted to protocatechuate, inducible protocatechuate 3,4-dioxygenase and/or protocatechuate 4,5-dioxygenase was expressed. (springer.com)
  • Synthetic 6-aryl-2-hydroxy-6-ketohexa-2,4-dienoic acid substrates for C-C hydrolase BphD: investigation of a general base catalytic mechanism. (warwick.ac.uk)
  • Dioxygenases catalyze the incorporation of both atoms of molecular oxygen into substrates. (expasy.org)
  • The substrates of ring-cleavage dioxygenases can be classified into two groups according to the mode of scission of the aromatic ring. (expasy.org)
  • Other catechols are also substrates [J903]. (ormedmedical.us)
  • Catechol, 3- and 4-methylcatechol are poor substrates [G771]. (ormedmedical.us)
  • Docking studies with different para-substituted phenols and corresponding catechols inside of the active site of PH(IND) and BphC(LA-4) predicted that all the substrates should be transformed. (sigmaaldrich.com)
  • To quantify metabolism of different substituted catechols, we developed a semi-quantification method based on the conserved mass balance within our Escherichia coli system. (aiche.org)
  • Biosynthesis of catechol melanin from glycerol employing metabolically engineered Escherichia coli Microbial Cell Factories, 15, 161. (unam.mx)
  • The two enzymes were identified to be a part of two separate catechol dioxygenase families: 1,2-CTD was classified as an intradiol dioxygenase while 2,3-CTD was classified as an extradiol dioxygenase. (wikipedia.org)
  • Two kinds of catechol dioxygenases can be found, which differ on the nature of the metal ion involved at the active site: Fe III allows an intradiol cleavage while Fe II or Mn II performs the extradiol one. (rsc.org)
  • A 1,2-dihydroxynaphthalene dioxygenase (ThnC) is responsible for the extradiol cleavage of the catechol derivative ( 1 ), followed by hydrolytic cleavage (by ThnD) of the C-C bond that is part of the alicyclic ring of the fission product, resulting in a long dicarboxylic acid with 10 carbon atoms ( 12 ). (pubmedcentralcanada.ca)
  • Intradiol enzymes cleave the aromatic ring between two hydroxyl groups, whereas extradiol enzymes cleave the aromatic ring between a hydroxylated carbon and another adjacent nonhydroxylated carbon [ 1 ]. (expasy.org)
  • Purification, biochemical properties and substrate specificity of a catechol 1,2-dioxygenase from a phenol degrading Acinetobacter radioresistens. (semanticscholar.org)
  • Isolation and partial characterization of extracellular NADPH-dependent phenol hydroxylase oxidizing phenol to catechol in Comamonas testosteroni. (nel.edu)
  • Turek M, Vilimkova L, Kremlackova V, Paca J, Halecky M, Paca J, Stiborova M. Isolation and partial characterization of extracellular NADPH-dependent phenol hydroxylase oxidizing phenol to catechol in Comamonas testosteroni. (nel.edu)
  • Isolation and partial characterization of catechol 1,2-dioxygenase of phenol degrading yeast Candida tropicalis. (nel.edu)
  • The activity of 2,3-dioxygenase was detected after phenol induction. (bireme.br)
  • Multistep conversion of para-substituted phenols by phenol hydroxylase and 2,3-dihydroxybiphenyl 1,2-dioxygenase. (sigmaaldrich.com)
  • A multistep conversion system of para-substituted phenols by recombinant phenol hydroxylase (PH(IND)) and 2,3-dihydroxybiphenyl 1,2-dioxygenase (BphC(LA-4)) was constructed in this study. (sigmaaldrich.com)
  • These bacteria subsequently employ 1,2-CTD in the last step of the degradation of aromatic compounds to aliphatic products. (wikipedia.org)
  • Six species of free-living nitrogen fixing bacteria, Azomonas agilis, Azospirillum brasilense, Azospirillum lipoferum, Azotobacter chroococcum, Azotobacter vinelandii , and Beijerinckia mobilis , were surveyed for their ability to grow and fix N 2 using aromatic compounds as sole carbon and energy source. (springer.com)
  • Cleavage of aromatic rings is one of the most important function of dioxygenases. (expasy.org)
  • The purpose of this study was purification and characterization of catechol 1,2-dioxygenase from Geobacillus sp. (semanticscholar.org)
  • This study represents the first extensive characterization of catechols adducts of 1,2-CTDs. (rcsb.org)
  • strain JS42 to 2-nitrotoluene. (nih.gov)
  • strain JS42 is able to utilize 2-nitrotoluene (2NT) as its sole carbon, nitrogen, and energy source. (nih.gov)
  • Strain KB2 is from among different Stenotrophomonas maltophilia strains the first one described as exhibiting the activities of three types of dioxygenases depending on the structure of the inducer. (bireme.br)
  • Protocatechuate 3,4-dioxygenase was found in crude cell extracts of this strain after incubation with 4-hydroxybenzoic acid, protocatechuic acid and vanillic acid. (bireme.br)
  • Because of broad spectrum of dioxygenases' types that Stenotrophomonas maltophilia KB2 can exhibit, this strain appears to be very powerful and useful tool in the biotreatment of wastewaters and in soil decontamination. (bireme.br)
  • The 16S rDNA analysis revealed 99% nucleotide similarity to that of the type strain of R.rhodochrous (Fig. 2) ( 10 ). (kenyon.edu)
  • The fatty acid biography of the strain PDB9T is also similar to the type strains of Rhodococcus species, but the amounts of C16:0, C18:1 cis9, 10-methyl-C18:0 show some variations. (kenyon.edu)
  • Strain AK 37, isolated from an oil-polluted soil in Hungary, degrades BTEX, an acronym standing for benzene, toluene, ethylbenzene, and o-xylenes ( 1 , 7 ), and strain PA has the capability to degrade benzothialzole ( 8 ). (kenyon.edu)
  • In the following section we will investigate in silico predictions of metabolic flux distribution in our catechol degrading E. coli strain through Flux Balance Analysis (FBA). (igem.org)
  • It is hypothesized that the combination of glucose and catechol that is taken up by the engineered strain may have a great affect on the flux distribution throughout the remaining metabolic network. (igem.org)
  • The Sphingomonas macrogolitabida strain TFA is a gram-negative bacterium that is able to grow on tetralin (1,2,3,4-tetrahydronaphthalene) as the only carbon and energy source ( 14 ). (pubmedcentralcanada.ca)
  • Bacterial strain XII, which belongs to the family Pseudomonad, utilizes 1-chloro-4-nitrobenzene as a sole source of carbon, nitrogen, and energy. (sciepub.com)
  • strain K‑804 [ 2 , 3 ] isolated from sorghum grain, and proved to be acutely toxic to Pekin ducklings, mice and rats [ 4 ]. (mdpi.com)
  • strain PAMC 25931 was characterized as eurypsychrophilic (both psychrophilic and mesotolerant) with a broad temperature range of 5-30°C both for anthranilate (2-aminobenzoate) degradation and concomitant cell growth. (elsevier.com)
  • For the second-step conversion, the formation rates of semialdehydes by strain BphC(LA-4) were as follows: 5-fluoro-HODA>5-chloro-HODA>2-hydroxy-5-nitro-ODA>5-bromo-HODA>2-hydroxy-5-methyl-ODA. (sigmaaldrich.com)
  • These events, apparently confined in proximity to 1 to 2 kb (the length of a typical gene) by the length of a replication fork, appear to be RecA independent ( 9 , 31 ), in contrast to large sequence-guided mutations which depend upon RecA-mediated recombination. (asm.org)
  • An important gene associated with Mycobacterium Fortuitum is HSPD1 (Heat Shock Protein Family D (Hsp60) Member 1), and among its related pathways/superpathways are Sudden Infant Death Syndrome (SIDS) Susceptibility Pathways and Cytokine production by Th17 cells in CF (Mouse model) . (malacards.org)
  • Chlorocatechol 1,2-dioxygenase (EC 1.13.11.1 ) (gene tfdC). (expasy.org)
  • A total of 11,195 protein-coding genes were predicted including a diverse group of gene families involved in hydrocarbon degradation pathways like dioxygenases and cytochrome P450. (biomedcentral.com)
  • Intradiol dioxygenases catalyze the critical ring-cleavage step in the conversion of catecholate derivatives to citric acid cycle intermediates. (rcsb.org)
  • Intradiol dioxygenases require a nonheme ferric ion as a cofactor. (expasy.org)
  • 2. Role of oxygen in the metabolic regulation : Besides the well known … More role of oxygen in the energy metabolism, oxygen has been shown to play an important physiological role in metabolic regulation through oxygenase-catalyzed reactions. (nii.ac.jp)
  • and catechol and gentisate are key intermediates in the two known pathways ( 9 , 24 , 27 , 65 ). (asm.org)
  • These data reveal the role of CatA2 as a type of metabolic safety valve for excess catechol that alleviates the metabolic conflict generated by simultaneous expression of the meta and ortho pathways, thereby facilitating their co-existence. (csic.es)
  • To address the decontamination of (1) toxic organic compounds such as naphthene and catechol found in the tailings ponds, natural pathways that can break down the toxic chemicals can be utilized. (igem.org)
  • Structure and Function of Dioxygenases. (umn.edu)
  • 1. Investigation of the research on the metabolic regulation in China : A five member delegation from our University visited Changchun city and Jilin city in China in August 1990, and delivered lectures at Central Hospital of Changchun city and Jilin Medical College. (nii.ac.jp)
  • Though experimentally determined transport rates of these energy sources are not yet available, we will do a preliminary analysis of the response to catechol uptake by investigating different points on the predicted metabolic landscape. (igem.org)
  • The predicted optimal uptake of catechol through FBA of the E. coli metabolic model is 2.52 mmol/gDW/h, resulting in a growth rate of 0.744 h-1. (igem.org)
  • This is a prediction of the optimal catechol input into the metabolic system in order to produce the maximum amount of biomass components, taking into account the steady-state balancing of all associated metabolic compounds and cofactors. (igem.org)
  • Though prediction of metabolic flux and microbial growth rate has been shown to be generally accurate through this method, experimental usage of catechol may be significantly higher or lower than the purely stoichiometric prediction. (igem.org)
  • This is because the input of catechol into the metabolic network in vivo may trigger metabolic regulation at the expense of the assumed cellular objective (biomass production). (igem.org)
  • Furthermore, even at a given combination of glucose and catechol uptake rate, there is a degree of variability associated with the in silico prediction of internal metabolic fluxes. (igem.org)
  • However, in this study we will consider the changes in predicted metabolic reaction variability in order to gain insight into the metabolic response to different combinations of glucose and catechol uptake. (igem.org)
  • In 2010, the Lethbridge iGEM team confirmed that xylE when introduce to E. coli hydrolyzes catechol to 2-HMS demonstrating and that this critical step can successfully be performed to convert toxic chemicals to metabolic intermediates 7 . (igem.org)
  • a non-heme iron dioxygenase with broad substrate tolerance. (cbrc.jp)
  • Crystal Structures of Substrate and Substrate Analog Complexes of Protocatechuate 3,4-dioxygenase: Endogenous Fe+3 Ligand Displacement in Response to Substrate Binding. (rcsb.org)
  • The molecular structures of complexes 1 and 2 were determined by X-ray crystallography. (diva-portal.org)
  • Diiron complexes 1 and 2 contain the same bis(u-alkoxo)diiron diamond core. (diva-portal.org)
  • The iron(III) complexes of tripodal phenolate ligands containing N 3 O and N 2 O 2 donor sets represent the metal binding region of the iron proteins. (ias.ac.in)
  • In our laboratory iron(III) complexes of mono- and bisphenolate ligands have been studied successfully as structural and functional models for the intradiol-cleaving catechol dioxygenase enzymes. (ias.ac.in)
  • One of the bis -phenolato complexes contains a FeN 2 O 2 Cl chromophore with a novel trigonal bipyramidal coordination geometry. (ias.ac.in)
  • The nature of heterocyclic rings of the ligands and the methyl substituents on them regulate the electronic spectral features, Fe III /Fe II redox potentials and catechol cleavage activity of the complexes. (ias.ac.in)
  • Four of the complexes catalyze the oxidative cleavage of H 2 DBC by molecular oxygen to yield intradiol cleavage products. (ias.ac.in)
  • In our laboratory, copper(II) complexes of bidentate ligands have been reported as catalysts for the fixation of CO 2 . (ias.ac.in)
  • These copper(II) complexes have successfully fixed atmospheric CO 2 as CO 3 2 2- by using Et 3 N as sacrificial reducing agent and afforded [Cu(L)CO 3 (H 2 O)]. The CO 3 2 2- bound complex has shown a distorted square pyramidal geometry (τ, 0.369) around copper(II) center via the coordination of only one ligand unit, a carbonate, and water molecules. (ias.ac.in)
  • An experimental approach to this question was presented by the heat-sensitive phenotype conferred by pcaG1102 , a 30-bp deletion in one of the structural genes for Acinetobacter baylyi protocatechuate 3,4-dioxygenase, which is required for growth with quinate. (asm.org)
  • An EPR, thermostability and pH-dependence study of wild-type and mutant forms of catechol 1,2 dioxygenase from Acinetobacter radioresistens S13. (unito.it)
  • Although the genus, Rhodococcus, implies the coccus shape of the organisms, the bacteria surprisingly show extensive polymorphism (Fig. 1. (kenyon.edu)
  • 2008. Roles of ring-hydroxylating dioxygenases in styrene and benzene catabolism in Rhodococcus jostii RHA1. (ubc.ca)
  • A. chroococcum expressed only ortho cleavage dioxygenases during growth on naphthalene and 4-toluate and only meta cleavage dioxygenases on the other aromatics. (springer.com)
  • B. mobilis expressed only ortho cleavage dioxygenases. (springer.com)
  • A quantitative relationship (R=0.966) between the ln k(cat) and the calculated electronic parameter E(HOMO) was obtained for catechol, 3-methylcatechol, 4-methylcatechol, pyrogallol, 3-chlorocatechol, 4-chlorocatechol. (rcsb.org)
  • Cleavage of pyrogallol by non-heme iron-containing dioxygenases. (cbrc.jp)
  • 6 This is the case of catechol dioxygenase, which catalyses the cleavage of catechol derivatives. (rsc.org)
  • Binds 1 Fe(3+) ion per subunit. (cbrc.jp)
  • The catalytic mechanism of catechol 1,2-dioxygenase was elucidated using a combination of O18 labeling experiments and crystallography. (wikipedia.org)
  • 6-9 In particular, the manganese-dependent dioxygenase (MndD) is a system that has attracted much attention since its catalytic mechanism is still under discussion. (rsc.org)
  • 2009. Studies of a ring-cleaving dioxygenase illuminate the role of cholesterol metabolism in the pathogenesis of Mycobacterium tuberculosis. (ubc.ca)
  • The reaction of 1 and catechol in the presence of excess triethylamine gave the catecholate (CAT) chelate b is(u -al koxo) -bridged dinuclear iron(Ill) complex [Fe(L)(CAT)12 (2). (diva-portal.org)
  • The reaction rate of the reactive intermediate [Fe(HL)(DBC)](+) with dioxygen is 0.38 M-1 s(-1) determined by kinetic studies. (diva-portal.org)
  • 3-9 Although the reaction with dioxygen is thermodynamically favourable, the electronic configuration of the O 2 molecule creates a strong kinetic barrier that requires activation, which is useful to prevent any spontaneous reaction of O 2 with organic molecules. (rsc.org)
  • Stereochemistry of the reaction catalysed by 2-hydroxy-6-keto-6-phenyl-hexa-2,4-dienoic acid 5,6-hydrolase (BphD). (warwick.ac.uk)
  • For instance, the reaction of methyl carbonate 1 with methyl acrylate (2) leads to the coupling product 3. (docme.ru)
  • 2637-2638 New Evidence Concerning the Structure of Amorphous Red Phosphorus P10 COMMUNICATIONS High regioselectivity, good yields, and mild reaction conditions are the advantages of the reaction of iminium salts 1 with imines 2 to afford the Mannich bases 3. (docme.ru)
  • Rapid Reaction Studies on the Oxygenation Reactions of Catechol Dioxygenase", Walsh, T. A. (umn.edu)
  • 1,2-CTD uses Fe3+ as a cofactor to cleave the carbon-carbon bond between the phenolic hydroxyl groups of catechol, thus yielding muconic acid as its product. (wikipedia.org)
  • Most of the 2,4-D plasmids were found in strains isolated by enrichment on 2,4-D as the sole source of carbon and energy, and some of them were found to take part in the degradation of a herbicide with a similar structure, 2-methyI-4-chlorophenoxyacetic acid. (asmscience.org)
  • The physiological properties of te sted strains were analyzed by Gram stain, activity of catechol dioxygenase. (cnu.edu.tw)
  • 2007. Specificity fingerprinting of retaining -1,4-glycanases in Cellulomonas fimi secretome using two fluorescent mechanism-based probes. (ubc.ca)
  • The benzene ring of catechol is cleaved by a 1,2-dioxygenase or 2,3-dioxygenase ( 1 , 40 ), whereas gentisate is cleaved by gentisate 1,2-dioxygenase (GDO) ( 8 , 18 , 21 , 62 , 67 ). (asm.org)
  • Genome annotation revealed that four proteins are related to decolorization of azo dyes, such as azoreductase, laccases, benzene 1,2-dioxygenase, and catechol 1,2-dioxygenase. (pacb.com)
  • PCR amplification of WA46 DNA with degenerate primers for gentisate 1,2-dioxygenase (GDO) genes produced an amplicon of the expected size. (asm.org)
  • Its genome is 5.2 Mb with 67.8% G+C content, containing 4,822 protein coding genes, three encode rRNA : one for 16S rRNA and two for 23S rRNA (GenBank database accession number AHBW00000000.1 ) ( 1 ). (kenyon.edu)
  • All heteroatoms (N3O) of the ligand are coordinated to the iron center in complex 1 with two pyridine nitrogen atoms on the axial bonds, while one of the pyridyl arms of the ligand is left uncoordinated in complex 2. (diva-portal.org)
  • Figure 1 shows predicted E. coli growth at various combinations of glucose and catechol uptake. (igem.org)
  • Because of its extraordinary position as a preferred model in biochemical genetics, molecular biology, and biotechnology, E. coli K-12 was the earliest organism to be suggested as a candidate for whole genome sequencing ( 1 , 2 ). (sciencemag.org)
  • In preliminary experiments we established the baseline sensitivity of E.coli (DH5a) exposed to 50mM catechol in liquid media over a six hour time course. (igem.org)
  • Although the effects of Catechol exposure in E.coli and P. putida have previously been investigated [Park et al. (igem.org)
  • Figure 1: A. Growth of E.coli DH5a on LB plates (1x10 -5 dilution) following exposure to minimal media and minimal media with 50mM catechol in solution. (igem.org)
  • B. Survival curves of E. coli DH5a exposed to catechol over a six hour time course. (igem.org)
  • Appl Environ Microbiol (1980) 39 (1): 58-67. (ethz.ch)
  • [email protected] b Instituto de Ciencia Molecular, Universitat de València, C/Catedrático José Beltrán 2, 46980 Paterna, València, Spain. (rsc.org)
  • In comparision, the oxidation of 4- t -butyl-catechol (4-TBC) that always required a basic media led to a total oxidation into the ortho -quinone, contrary to the molecular analogue. (rsc.org)
  • 2 In particular, several metalloenzymes called oxygenases perform the oxidation of organic compounds by activating molecular dioxygen. (rsc.org)
  • Molecular mechanisms of the involvement of ceramide in apoptosis in pancreatic β-cells and its role in the formation of insulin resistance in pathogenesis of type 2 diabetes are reviewed. (chemweb.com)
  • Catechol 1,2-dioxygenase (CTD) and protocatechuate 3,4-dioxygenase (PCD) are bacterial non-heme iron enzymes, which catalyse the oxidative cleavage of catechols to cis, cis -muconic acids with the incorporation of molecular oxygen via a mechanism involving a high-spin ferric centre. (ias.ac.in)
  • SDS-PAGE of cell free extracts showed two prominent bands close to molecular weight of catechol 1,2-dioxygenase. (iisc.ernet.in)
  • Molecular structure of ochratoxins naturally produced by filamentous fungi [ 2 , 5 , 6 ]. (mdpi.com)
  • In: Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1293 (2). (iisc.ernet.in)
  • Nitrobenzene and its derivatives (NBDs) are a group of toxic chemicals that are widespread in the environment owing to their worldwide use [ 1 ]. (hindawi.com)
  • In contrast, 2,3-CTD utilizes Fe2+ as a cofactor to cleave the carbon-carbon bond adjacent to the phenolic hydroxyl groups of catechol, thus yielding 2-hydroxymuconaldehye as its product. (wikipedia.org)
  • The second catechol hydroxyl group on carbon 3 (C3) is coordinated to Fe3+ after its deprotonation by the Tyr200 ligand. (wikipedia.org)
  • The fixation of carbon dioxide (CO 2 ) is an important global challenge. (ias.ac.in)
  • HO 2 ) residue to arachidonic acid (i.e. 5 Z ,8 Z ,11 Z ,14 Z -eicosatetraenoic acid) at carbon 5 of its 1,4 diene group (i.e. its 5 Z ,8 Z double bonds) to form 5 (S) -hydroperoxy-6 E ,8 Z ,11 Z ,14 Z -eicosatetraenoic acid (i.e. 5 S -HpETE). (wikipedia.org)
  • Salicylate is converted to catechol and gentisate by salicylate hydroxylase ( 4 , 6 ) and salicylate 5-hydroxylase ( 22 , 27 ), respectively. (asm.org)