Catechols: A group of 1,2-benzenediols that contain the general formula R-C6H5O2.Catechol 2,3-Dioxygenase: Catalyzes the oxidation of catechol to 2-hydroxymuconate semialdehyde in the carbazole and BENZOATE degradation via HYDROXYLATION pathways. It also catalyzes the conversion of 3-methylcatechol to cis, cis-2-hydroxy-6-oxohept-2,4-dienoate in the TOLUENE and XYLENE degradation pathway. This enzyme was formerly characterized as EC 1.13.1.2.Dioxygenases: Non-heme iron-containing enzymes that incorporate two atoms of OXYGEN into the substrate. They are important in biosynthesis of FLAVONOIDS; GIBBERELLINS; and HYOSCYAMINE; and for degradation of AROMATIC HYDROCARBONS.Catechol 1,2-Dioxygenase: An enzyme that catalyzes the oxidation of catechol to muconic acid with the use of Fe3+ as a cofactor. This enzyme was formerly characterized as EC 1.13.1.1 and EC 1.99.2.2.Oxygenases: Oxidases that specifically introduce DIOXYGEN-derived oxygen atoms into a variety of organic molecules.Estrogens, Catechol: 2- or 4-Hydroxyestrogens. Substances that are physiologically active in mammals, especially in the control of gonadotropin secretion. Physiological activity can be ascribed to either an estrogenic action or interaction with the catecholaminergic system.4-Hydroxyphenylpyruvate Dioxygenase: An enzyme that catalyzes the conversion of 4-hydroxyphenylpyruvate plus oxygen to homogentisic acid and carbon dioxide. EC 1.13.11.27.Protocatechuate-3,4-Dioxygenase: An enzyme that catalyzes the oxidation of protocatechuate to 3-carboxy-cis-cis-muconate in the presence of molecular oxygen. It contains ferric ion. EC 1.13.11.3.Cysteine Dioxygenase: An enzyme that catalyzes the conversion of L-CYSTEINE to 3-sulfinoalanine (3-sulfino-L-alanine) in the CYSTEINE metabolism and TAURINE and hypotaurine metabolic pathways.Tryptophan Oxygenase: A dioxygenase with specificity for the oxidation of the indoleamine ring of TRYPTOPHAN. It is a LIVER-specific enzyme that is the first and rate limiting enzyme in the kynurenine pathway of TRYPTOPHAN catabolism.Catechol O-Methyltransferase: Enzyme that catalyzes the movement of a methyl group from S-adenosylmethionone to a catechol or a catecholamine.Homogentisate 1,2-Dioxygenase: A mononuclear Fe(II)-dependent oxygenase, this enzyme catalyzes the conversion of homogentisate to 4-maleylacetoacetate, the third step in the pathway for the catabolism of TYROSINE. Deficiency in the enzyme causes ALKAPTONURIA, an autosomal recessive disorder, characterized by homogentisic aciduria, OCHRONOSIS and ARTHRITIS. This enzyme was formerly characterized as EC 1.13.1.5 and EC 1.99.2.5.3-Hydroxyanthranilate 3,4-Dioxygenase: An enzyme that catalyzes the conversion of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde. It was formerly characterized as EC 1.13.1.6.Pseudomonas: A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.KynureninePseudomonas putida: A species of gram-negative, aerobic bacteria isolated from soil and water as well as clinical specimens. Occasionally it is an opportunistic pathogen.Hydroxybenzoates: Benzoate derivatives substituted by one or more hydroxy groups in any position on the benzene ring.Rhodococcus: A bacterial genus of the order ACTINOMYCETALES.Phenol: An antiseptic and disinfectant aromatic alcohol.ChlorobenzenesSorbic Acid: Mold and yeast inhibitor. Used as a fungistatic agent for foods, especially cheeses.Catechol Oxidase: An enzyme of the oxidoreductase class that catalyzes the reaction between catechol and oxygen to yield benzoquinone and water. It is a complex of copper-containing proteins that acts also on a variety of substituted catechols. EC 1.10.3.1.Tryptophan: An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals.Chlorobenzoates: Benzoic acid or benzoic acid esters substituted with one or more chlorine atoms.Toluene: A widely used industrial solvent.Benzoates: Derivatives of BENZOIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxybenzene structure.Mixed Function Oxygenases: Widely distributed enzymes that carry out oxidation-reduction reactions in which one atom of the oxygen molecule is incorporated into the organic substrate; the other oxygen atom is reduced and combined with hydrogen ions to form water. They are also known as monooxygenases or hydroxylases. These reactions require two substrates as reductants for each of the two oxygen atoms. There are different classes of monooxygenases depending on the type of hydrogen-providing cosubstrate (COENZYMES) required in the mixed-function oxidation.Sphingomonas: A genus of gram-negative, aerobic, rod-shaped bacteria characterized by an outer membrane that contains glycosphingolipids but lacks lipopolysaccharide. They have the ability to degrade a broad range of substituted aromatic compounds.Burkholderia: A genus of gram-negative, aerobic, rod-shaped bacteria. Organisms in this genus had originally been classified as members of the PSEUDOMONAS genus but overwhelming biochemical and chemical findings indicated the need to separate them from other Pseudomonas species, and hence, this new genus was created.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Benzoic Acid: A fungistatic compound that is widely used as a food preservative. It is conjugated to GLYCINE in the liver and excreted as hippuric acid.Gentisates: Salts and esters of gentisic acid.Alcaligenes: A genus of gram-negative, aerobic, motile bacteria that occur in water and soil. Some are common inhabitants of the intestinal tract of vertebrates. These bacteria occasionally cause opportunistic infections in humans.Benzene: Toxic, volatile, flammable liquid hydrocarbon byproduct of coal distillation. It is used as an industrial solvent in paints, varnishes, lacquer thinners, gasoline, etc. Benzene causes central nervous system damage acutely and bone marrow damage chronically and is carcinogenic. It was formerly used as parasiticide.Adipates: Derivatives of adipic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a 1,6-carboxy terminated aliphatic structure.Equilenin: An estrogenic steroid produced by HORSES. It has a total of five double bonds in the A- and B-ring. High concentration of equilenin is found in the URINE of pregnant mares.Comamonas: A genus of gram-negative, straight or slightly curved rods which are motile by polar flagella and which accumulate poly-beta-hydroxybutyrate within the cells.Genes, Bacterial: The functional hereditary units of BACTERIA.Homogentisic AcidOxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Acinetobacter: A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.Pyrogallol: A trihydroxybenzene or dihydroxy phenol that can be prepared by heating GALLIC ACID.Brevibacterium: A gram-positive organism found in dairy products, fresh and salt water, marine organisms, insects, and decaying organic matter.Hydrocarbons, Aromatic: Organic compounds containing carbon and hydrogen in the form of an unsaturated, usually hexagonal ring structure. The compounds can be single ring, or double, triple, or multiple fused rings.Phenols: Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.Ralstonia: A genus in the family BURKHOLDERIACEAE, comprised of many species. They are associated with a variety of infections including MENINGITIS; PERITONITIS; and URINARY TRACT INFECTIONS.HydroquinonesAmino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Planococcus Bacteria: A genus of coccoid bacteria in the family PLANOCOCCACEAE. They are widely distributed in various habitats including sea water, freshwater ponds, cyanobacterial mats, and in marine animals.Iron: A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.Hydroxylation: Placing of a hydroxyl group on a compound in a position where one did not exist before. (Stedman, 26th ed)Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Parabens: Methyl, propyl, butyl, and ethyl esters of p-hydroxybenzoic acid. They have been approved by the FDA as antimicrobial agents for foods and pharmaceuticals. (From Hawley's Condensed Chemical Dictionary, 11th ed, p872)Polychlorinated Biphenyls: Industrial products consisting of a mixture of chlorinated biphenyl congeners and isomers. These compounds are highly lipophilic and tend to accumulate in fat stores of animals. Many of these compounds are considered toxic and potential environmental pollutants.Gram-Negative Aerobic Rods and Cocci: A group of gram-negative bacteria consisting of rod- and coccus-shaped cells. They are both aerobic (able to grow under an air atmosphere) and microaerophilic (grow better in low concentrations of oxygen) under nitrogen-fixing conditions but, when supplied with a source of fixed nitrogen, they grow as aerobes.Ferredoxins: Iron-containing proteins that transfer electrons, usually at a low potential, to flavoproteins; the iron is not present as in heme. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Kinetics: The rate dynamics in chemical or physical systems.CresolsBurkholderiaceae: A family of gram negative, aerobic, non-sporeforming, rod-shaped bacteria.3-Hydroxyanthranilic Acid: An oxidation product of tryptophan metabolism. It may be a free radical scavenger and a carcinogen.gamma-Butyrobetaine Dioxygenase: An enzyme that catalyzes the HYDROXYLATION of gamma-butyrobetaine to L-CARNITINE. It is the last enzyme in the biosynthetic pathway of L-CARNITINE and is dependent on alpha-ketoglutarate; IRON; ASCORBIC ACID; and OXYGEN.Arthrobacter: A genus of asporogenous bacteria isolated from soil that displays a distinctive rod-coccus growth cycle.Alkaptonuria: An inborn error of amino acid metabolism resulting from a defect in the enzyme HOMOGENTISATE 1,2-DIOXYGENASE, an enzyme involved in the breakdown of PHENYLALANINE and TYROSINE. It is characterized by accumulation of HOMOGENTISIC ACID in the urine, OCHRONOSIS in various tissues, and ARTHRITIS.Xylenes: A family of isomeric, colorless aromatic hydrocarbon liquids, that contain the general formula C6H4(CH3)2. They are produced by the destructive distillation of coal or by the catalytic reforming of petroleum naphthenic fractions. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Ketoglutaric Acids: A family of compounds containing an oxo group with the general structure of 1,5-pentanedioic acid. (From Lehninger, Principles of Biochemistry, 1982, p442)Biphenyl CompoundsSalicylates: The salts or esters of salicylic acids, or salicylate esters of an organic acid. Some of these have analgesic, antipyretic, and anti-inflammatory activities by inhibiting prostaglandin synthesis.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Intramolecular Lyases: Enzymes of the isomerase class that catalyze reactions in which a group can be regarded as eliminated from one part of a molecule, leaving a double bond, while remaining covalently attached to the molecule. (From Enzyme Nomenclature, 1992) EC 5.5.Ferrous Compounds: Inorganic or organic compounds that contain divalent iron.ToluidinesEnzyme Induction: An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.ortho-Aminobenzoates: Benzoic acids, salts, or esters that contain an amino group attached to carbon number 2 or 6 of the benzene ring structure.Vanillic Acid: A flavoring agent. It is the intermediate product in the two-step bioconversion of ferulic acid to vanillin. (J Biotechnol 1996;50(2-3):107-13).Biotransformation: The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.Bacterial Proteins: Proteins found in any species of bacterium.Sulfanilic Acids: Aminobenzenesulfonic acids. Organic acids that are used in the manufacture of dyes and organic chemicals and as reagents.PhenanthrenesBase Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Iron-Sulfur Proteins: A group of proteins possessing only the iron-sulfur complex as the prosthetic group. These proteins participate in all major pathways of electron transport: photosynthesis, respiration, hydroxylation and bacterial hydrogen and nitrogen fixation.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Naphthalenes: Two-ring crystalline hydrocarbons isolated from coal tar. They are used as intermediates in chemical synthesis, as insect repellents, fungicides, lubricants, preservatives, and, formerly, as topical antiseptics.Benzene DerivativesHydroxyestrones: Estrone derivatives substituted with one or more hydroxyl groups in any position. They are important metabolites of estrone and other estrogens.Quinolinic Acid: A metabolite of tryptophan with a possible role in neurodegenerative disorders. Elevated CSF levels of quinolinic acid are correlated with the severity of neuropsychological deficits in patients who have AIDS.Spectrophotometry: The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.Pseudomonas stutzeri: A species of gram-negative bacteria in the genus PSEUDOMONAS, containing multiple genomovars. It is distinguishable from other pseudomonad species by its ability to use MALTOSE and STARCH as sole carbon and energy sources. It can degrade ENVIRONMENTAL POLLUTANTS and has been used as a model organism to study denitrification.Delftia acidovorans: A species of gram-negative rod-shaped bacteria found ubiquitously and formerly called Comamonas acidovorans and Pseudomonas acidovorans. It is the type species of the genus DELFTIA.Electron Spin Resonance Spectroscopy: A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.Chlorophenols: Phenols substituted with one or more chlorine atoms in any position.Acinetobacter calcoaceticus: A species of gram-negative, aerobic bacteria found in soil and water. Although considered to be normally nonpathogenic, this bacterium is a causative agent of nosocomial infections, particularly in debilitated individuals.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Oxidoreductases: The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)3,4-Dihydroxyphenylacetic Acid: A deaminated metabolite of LEVODOPA.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Multigene Family: A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)Burkholderia cepacia: A species of BURKHOLDERIA considered to be an opportunistic human pathogen. It has been associated with various types of infections of nosocomial origin.Oxygen: An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.Hydrolases: Any member of the class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting molecules, e.g., ESTERASES, glycosidases (GLYCOSIDE HYDROLASES), lipases, NUCLEOTIDASES, peptidases (PEPTIDE HYDROLASES), and phosphatases (PHOSPHORIC MONOESTER HYDROLASES). EC 3.beta-Carotene 15,15'-Monooxygenase: A monooxygenase that catalyzes the conversion of BETA-CAROTENE into two molecules of RETINAL. It was formerly characterized as EC 1.13.11.21 and EC 1.18.3.1.Oxygen Isotopes: Stable oxygen atoms that have the same atomic number as the element oxygen, but differ in atomic weight. O-17 and 18 are stable oxygen isotopes.Enzyme Reactivators: Compounds which restore enzymatic activity by removing an inhibitory group bound to the reactive site of the enzyme.Tyrosinemias: A group of disorders which have in common elevations of tyrosine in the blood and urine secondary to an enzyme deficiency. Type I tyrosinemia features episodic weakness, self-mutilation, hepatic necrosis, renal tubular injury, and seizures and is caused by a deficiency of the enzyme fumarylacetoacetase. Type II tyrosinemia features INTELLECTUAL DISABILITY, painful corneal ulcers, and keratoses of the palms and plantar surfaces and is caused by a deficiency of the enzyme TYROSINE TRANSAMINASE. Type III tyrosinemia features INTELLECTUAL DISABILITY and is caused by a deficiency of the enzyme 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE. (Menkes, Textbook of Child Neurology, 5th ed, pp42-3)Naphthols: Naphthalene derivatives carrying one or more hydroxyl (-OH) groups at any ring position. They are often used in dyes and pigments, as antioxidants for rubber, fats, and oils, as insecticides, in pharmaceuticals, and in numerous other applications.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Catalysis: The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.2,4-Dichlorophenoxyacetic Acid: An herbicide with irritant effects on the eye and the gastrointestinal system.Stereoisomerism: The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)MaleatesSoil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.Trichloroethylene: A highly volatile inhalation anesthetic used mainly in short surgical procedures where light anesthesia with good analgesia is required. It is also used as an industrial solvent. Prolonged exposure to high concentrations of the vapor can lead to cardiotoxicity and neurological impairment.Pseudomonas pseudoalcaligenes: A species of gram-negative bacteria in the genus PSEUDOMONAS. All strains can utilize FRUCTOSE for energy. It is occasionally isolated from humans and some strains are pathogenic to WATERMELON.Aniline CompoundsSiderophores: Low-molecular-weight compounds produced by microorganisms that aid in the transport and sequestration of ferric iron. (The Encyclopedia of Molecular Biology, 1994)Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Spectrophotometry, Ultraviolet: Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Quinones: Hydrocarbon rings which contain two ketone moieties in any position. They can be substituted in any position except at the ketone groups.Gram-Negative Aerobic Bacteria: A large group of aerobic bacteria which show up as pink (negative) when treated by the gram-staining method. This is because the cell walls of gram-negative bacteria are low in peptidoglycan and thus have low affinity for violet stain and high affinity for the pink dye safranine.Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Monophenol Monooxygenase: An enzyme of the oxidoreductase class that catalyzes the reaction between L-tyrosine, L-dopa, and oxygen to yield L-dopa, dopaquinone, and water. It is a copper protein that acts also on catechols, catalyzing some of the same reactions as CATECHOL OXIDASE. EC 1.14.18.1.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Isomerases: A class of enzymes that catalyze geometric or structural changes within a molecule to form a single product. The reactions do not involve a net change in the concentrations of compounds other than the substrate and the product.(from Dorland, 28th ed) EC 5.Gallic Acid: A colorless or slightly yellow crystalline compound obtained from nutgalls. It is used in photography, pharmaceuticals, and as an analytical reagent.1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt: A colorimetric reagent for iron, manganese, titanium, molybdenum, and complexes of zirconium. (From Merck Index, 11th ed)Pseudomonas mendocina: A species of gram-negative bacteria in the genus PSEUDOMONAS, which is found in SOIL and WATER.Soil Pollutants: Substances which pollute the soil. Use for soil pollutants in general or for which there is no specific heading.Benzofurans: Compounds that contain a BENZENE ring fused to a furan ring.Kynurenine 3-Monooxygenase: An NADPH-dependent flavin monooxygenase that plays a key role in the catabolism of TRYPTOPHAN by catalyzing the HYDROXYLATION of KYNURENINE to 3-hydroxykynurenine. It was formerly characterized as EC 1.14.1.2 and EC 1.99.1.5.Interferon-gamma: The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.Nonheme Iron Proteins: Proteins, usually acting in oxidation-reduction reactions, containing iron but no porphyrin groups. (Lehninger, Principles of Biochemistry, 1993, pG-10)Adrenochrome: Pigment obtained by the oxidation of epinephrine.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Multienzyme Complexes: Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Models, Chemical: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.BenzophenonesSequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Gas Chromatography-Mass Spectrometry: A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Normetanephrine: A methylated metabolite of norepinephrine that is excreted in the urine and found in certain tissues. It is a marker for tumors.Carboxy-Lyases: Enzymes that catalyze the addition of a carboxyl group to a compound (carboxylases) or the removal of a carboxyl group from a compound (decarboxylases). EC 4.1.1.Comamonadaceae: A family of gram-negative aerobic bacteria in the class BETA PROTEOBACTERIA, encompassing the acidovorans rRNA complex. Some species are pathogenic for PLANTS.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Taurine: A conditionally essential nutrient, important during mammalian development. It is present in milk but is isolated mostly from ox bile and strongly conjugates bile acids.
3-MethylcatecholExtradiol Cleavage of 3-Methylcatechol by Catechol 1,2-Dioxygenase from Various Microorganisms. C. T. Hou, R. Patel and M. O. ... The isofunctional enzymes of catechol 1,2-dioxygenase from species of Acinetobacter, Pseudomonas, Nocardia, Alcaligenes and ... The enzyme 1,2-dihydroxy-6-methylcyclohexa-3,5-dienecarboxylate dehydrogenase uses 1,2-dihydroxy-6-methylcyclohexa-3,5- ... Higson FK, Focht DD (1992). "Degradation of 2-methylbenzoic acid by Pseudomonas cepacia MB2". Appl. Environ. Microbiol. 58 (1 ...
... dioxygenases MeSH D08.811.682.690.416.277 --- catechol 1,2-dioxygenase MeSH D08.811.682.690.416.305 --- catechol 2,3- ... dioxygenase MeSH D08.811.682.690.416.319 --- cysteine dioxygenase MeSH D08.811.682.690.416.326 --- homogentisate 1,2- ... dioxygenase MeSH D08.811.682.690.416.328 --- 3-hydroxyanthranilate 3,4-dioxygenase MeSH D08.811.682.690.416.330 --- 4- ... catechol oxidase MeSH D08.811.682.690.708.125.500 --- monophenol monooxygenase MeSH D08.811.682.690.708.170 --- cytochrome p- ...
4-dihydroxybenzoate to produce catechol and CO2. The enzyme protocatechuate 3,4-dioxygenase uses 3,4-dihydroxybenzoate and O2 ... 4-dioxygenase Vanillate monooxygenase 3,4-dihydroxyphthalate decarboxylase 4,5-dihydroxyphthalate decarboxylase Degradation ... 23 (2): 201-208. doi:10.1016/j.tiv.2008.11.008. PMID 19095056. Anter J, Romero-Jiménez M, Fernández-Bedmar Z, Villatoro-Pulido ... Biosynthesis enzymes 3-dehydroshikimate dehydratase (3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate dehydrogenase ...
3-dioxygenase and 4-hydroxyphenylpyruvate dioxygenase all resemble glyoxalase I in structure. Finally, many proteins of unknown ... catechol 2,3-dioxygenase, 3,4-dihydroxyphenylacetate 2, ... 12 (2): 111-119. doi:10.1007/BF01026032. Larsen K, Aronsson A-C ... 3-demethylubiquinone-9 3-O-methyltransferase and numerous dioxygenases such as biphenyl-2,3-diol 1,2-dioxygenase, ... 190 (2): 685-96. PMID 14841219. Allen RE, Lo TW, Thornalley PJ (1993). "A simplified method for the purification of human red ...
3-dioxygenase, which mediates the electron transfer between catechol substrates and O2 to form a [M(II)(semiquinone)superoxo] ... "Characterization of an O2 Adduct of an Active Cobalt-Substituted Extradiol-Cleaving Catechol Dioxygenase". Journal of the ... "A Hyperactive Cobalt-Substituted Extradiol-Cleaving Catechol Dioxygenase". Journal of biological inorganic chemistry : JBIC : a ... 13 (2): 117-126. ISSN 0006-2944. PMID 1. Bose, K. S.; Sarma, R. H. (1975-10-27). "Delineation of the intimate details of the ...
... dependent dioxygenases. The catechol dioxygenases, some of the most well-studied dioxygenase enzymes, use dioxygen to cleave a ... Two important groups of mononuclear, non-heme iron dioxygenases are catechol dioxygenases and 2-oxoglutarate (2OG)- ... Catechol dioxygenases are further classified as being "extradiol" or "intradiol," and this distinction is based on mechanistic ... In this respect, these enzymes are reminiscent of the intradiol catechol dioxygenases whereby the metal centers activate the ...
3-dioxygenase (EC 1.13.11.2), and protocatechuate 3,4-dioxygenase (EC 1.13.11.3). The active site of catechol dioxygenases most ... Catechol dioxygenases are metalloprotein enzymes that carry out the oxidative cleavage of catechols. This class of enzymes ... 3-dioxygenase, is often used as a reporter to quantitate gene expression. An example of the reaction carried out by catechol 1, ... Catechol dioxygenases belong to the class of oxidoreductases and have several different substrate specificities, including ...
Muller R, Schmitt S, Lingens F (1982). "A novel non-heme iron-containing dioxygenase. Chloridazon-catechol dioxygenase from ... Chloridazon-catechol dioxygenase (EC 1.13.11.36) is an enzyme that catalyzes the chemical reaction 5-amino-4-chloro-2-(2,3- ... Muller R, Haug S, Eberspacher J, Lingens F (1977). "[Catechol 2,3-dioxygenase from pyrazon-degrading bacteria (author's transl ... The systematic name of this enzyme class is 5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone 1,2-oxidoreductase ( ...
Pascal, R; Huang, O (1986). "Reactions of 3-ethylcatechol and 3-(methylthio)catechol with catechol dioxygenases". Arch. Biochem ... a family of catechol dioxygenases that cleaves the bond between the phenolic hydroxyl groups of catechol using an Fe3+ cofactor ... Two families of dioxygenases were discovered by Osamu Hayaishi and Kizo Hashimoto in 1950: catechol 1,2-dioxygenase and ... catechol dioxygenase, pyrocatechase, pyrocatechol 1,2-dioxygenase, CD I, CD II) is an enzyme that catalyzes the oxidative ring ...
Junker, F.; Leisinger, T.; Cook, A.M. (1994). "3-Sulphocatechol dioxygenase and other dioxygenases (1.13.11.2 and 1.14.12.-) in ... 3-dioxygenase) is an enzyme with systematic name catechol:oxygen 2,3-oxidoreductase (decyclizing). This enzyme catalyses the ... Catechol 2,3-dioxygenase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and Cellular Biology ... Catechol 2,3-dioxygenase (EC 1.13.11.2, 2,3-pyrocatechase, catechol 2,3-oxygenase, catechol oxygenase, metapyrocatechase, ...
I Enzymatic formation of catechol from benzene". Biochemistry. 7 (7): 2653-62. doi:10.1021/bi00847a031. PMID 4298226. Molecular ... and benzene dioxygenase. This enzyme participates in naphthalene and anthracene degradation. It has 4 cofactors: FAD, Iron, ... In enzymology, a benzene 1,2-dioxygenase (EC 1.14.12.3) is an enzyme that catalyzes the chemical reaction benzene + NADH + H+ ... The systematic name of this enzyme class is benzene,NADH:oxygen oxidoreductase (1,2-hydroxylating). Other names in common use ...
... catechol + chloride + NAD+ + CO2 The 4 substrates of this enzyme are 2-chlorobenzoate, NADH, H+, and O2, whereas its 4 products ... are catechol, chloride, NAD+, and CO2. This enzyme belongs to the family of oxidoreductases, specifically those acting on ... In enzymology, a 2-chlorobenzoate 1,2-dioxygenase (EC 1.14.12.13) is an enzyme that catalyzes the chemical reaction 2- ... Fetzner S, Muller R, Lingens F (1989). "Degradation of 2-chlorobenzoate by Pseudomonas cepacia 2CBS". Biol. Chem. Hoppe-Seyler ...
Kobayashi S, Hayaishi O (1970). "Anthranilic acid conversion to catechol (Pseudomonas)". Methods Enzymol. 17A: 505-510. doi: ... catechol + CO2 + NAD(P)+ + NH3 The 5 substrates of this enzyme are anthranilate, NADH, NADPH, H+, and O2, whereas its 5 ... "Enzymatic formation of catechol from anthranilic acid". J. Biol. Chem. 239: 2204-2211. PMID 14209949. Molecular and Cellular ... products are catechol, CO2, NAD+, NADP+, and NH3. This enzyme belongs to the family of oxidoreductases, specifically those ...
Junca, H.; Plumeier, I.; Hecht, H. J. R.; Pieper, D. H. (December 2004). "Difference in kinetic behaviour of catechol 2,3- ... dioxygenase variants from a polluted environment". Microbiology. 150 (12): 4181-4187. doi:10.1099/mic.0.27451-0. PMID 15583170 ... 227 (1-2): 21-30. doi:10.1016/j.mce.2004.08.001. PMID 15501581. Görg, A.; Weiss, W.; Dunn, M. J. (December 2004). "Current two- ... In recent years, 2-D electrophoresis has been widely accepted as a standard procedure to separate complex protein mixtures in ...
other dioxygenase: Catechol dioxygenase. *Homogentisate 1,2-dioxygenase. *Cysteine dioxygenase. *4-Hydroxyphenylpyruvate ... 114 (1): 78-82. doi:10.1111/bcpt.12115. PMID 24020397.. *^ a b c d e f g Anwar Y, Sabir JS, Qureshi MI, Saini KS (2014). "5- ... ALOX5's dioxygenase activity adds a hydroperoxyl (i.e. HO2) residue to arachidonic acid (i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic ... dioxygenase activity. • metal ion binding. • protein binding. • oxidoreductase activity. • oxidoreductase activity, acting on ...
... related with the catechol and resorcinol groups and the oxidation is pH-dependent. The oxidation of the catechol 3',4'- ... Protocatechuic acid and hydroxyquinol undergo intradiol cleavage through protocatechuate 3,4-dioxygenase and hydroxyquinol 1,2- ... sulphation and methylation of the catechol group by catechol-O-methyl transferase, with only small amounts detected in plasma. ... dioxygenase to form β-carboxy cis, cis-muconic acid and maleyl acetate. Among fungi, degradation of catechin can be achieved by ...
Catechol dioxygenases. Department of Chemistry, Michigan State University, East Lansing, MI 488241322, U.S.A. ... Diversity of catechol 2,3-dioxygenase genes of bacteria responding to dissolved organic matter derived from different sources ... PCR isolation of catechol 2,3-dioxygenase gene fragments from environmental samples and their assembly into functional genes. ... The Catechol 1,2 Dioxygenase System of Acinetobacter radioresistens: Isoenzymes, Inductors and Gene localisation. Biol. Chem., ...
Catechol oxidase EC 1.10.3.1 Laccase EC 1.10.3.2 Category:EC 1.10.99 (with other acceptors) Category:EC 1.11.1 (peroxidases) ... 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) Category:EC 1.13.12 (With incorporation of one atom of oxygen (internal ... EC 1.14.12 Nitric oxide dioxygenase Category:EC 1.14.13 Nitric oxide synthase EC 1.14.13.39 Category:EC 1.14.14 Aromatase EC ... For a list of the EC numbers themselves, see: List of EC numbers (EC 1) List of EC numbers (EC 2) List of EC numbers (EC 3) ...
"The genes coding for the conversion of carbazole to catechol are flanked by IS6100 elements in Sphingomonas sp. strain XLDN2-5 ... 9a-dioxygenase, a three-component dioxygenase system of Pseudomonas resinovorans strain CA10". Appl. Environ. Microbiol. 68 (12 ... 9a-dioxygenase (EC 1.14.12.22, CARDO) is an enzyme with systematic name 9H-carbazole,NAD(P)H:oxygen oxidoreductase (2,3- ... 9a-dioxygenase catalyses the first reaction in the pathway of carbazole degradation. Nam, J.W.; Nojiri, H.; Noguchi, H.; ...
Junca, H.; Plumeier, I.; Hecht, H. J. R.; Pieper, D. H. (December 2004). "Difference in kinetic behaviour of catechol 2,3- ... dioxygenase variants from a polluted environment". Microbiology. 150 (12): 4181-4187. doi:10.1099/mic.0.27451-0. PMID 15583170. ... 1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] and a crucial step in gene expression profiling. ... 6 (1): 172-180. doi:10.1002/pmic.200500086. PMID 16317771.. *^ Stasyk, T.; Morandell, S.; Bakry, R.; Feuerstein, I.; Huck, C. W ...
... chloridazon-catechol dioxygenase EC 1.13.11.37: hydroxyquinol 1,2-dioxygenase EC 1.13.11.38: 1-hydroxy-2-naphthoate 1,2- ... 3-dioxygenase EC 1.13.11.18: sulfur dioxygenase EC 1.13.11.19: cysteamine dioxygenase EC 1.13.11.20: cysteine dioxygenase EC ... gibberellin 3b-dioxygenase EC 1.14.11.16: peptide-aspartate b-dioxygenase EC 1.14.11.17: taurine dioxygenase EC 1.14.11.18: ... 5-dioxygenase EC 1.14.12.8: 4-sulfobenzoate 3,4-dioxygenase EC 1.14.12.9: 4-chlorophenylacetate 3,4-dioxygenase EC 1.14.12.10: ...
For example, Rhodococci expresses dioxygenases, which can be used to degrade benzotrifluoride, a recalcitrant pollutant. ... catechol, benzoate, and protocatechuic acid. Rhodococci are also capable of accumulating heavy metal ions, such as radioactive ... "Degradation of benzotrifluoride via the dioxygenase pathway in Rhodococcus sp. 065240". Bioscience, Biotechnology, and ... ISBN 978-1-904455-30-1. [1]. McLeod MP, Warren RL, Hsiao WW, Araki N, Mihre M, Fernandes C, Miyazawa D, Wong W, Lillquist AL, ...
Heating catechin past its point of decomposition releases pyrocatechol (also called catechol), which explains the common origin ... leucoanthocyanidin dioxygenase), E11, anthocyanidin reductase. HSCoA, Coenzyme A. L-Tyr, L-tyrosine, L-Phe, L-phenylalanine. ... Figure 1:Schematic overview of the flavan-3-ol (-)-epicatechin biosynthesis in plants: Enzymes are indicated in blue, ... Retrieved 1 November 2014. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA)2, 3 European Food Safety Authority ( ...
Oxidoreductases: dioxygenases, including steroid hydroxylases (EC 1.14). 1.14.11: 2-oxoglutarate. *Prolyl hydroxylase ... 10 (6 Pt 2): 774-81. doi:10.1159/000073965. PMID 14631117.. *^ a b Halskau Ø, Ying M, Baumann A, Kleppe R, Rodriguez-Larrea D, ... 267 (1): 456-61. doi:10.1006/bbrc.1999.1945. PMID 10623641.. *^ Ono M, Okamoto M, Kawabe N, Umezawa H, Takeuchi T (Mar 1971). " ... 42 (1-2): 29-32. doi:10.1159/000132246. PMID 2872999.. *. Grima B, Lamouroux A, Boni C, Julien JF, Javoy-Agid F, Mallet J (1987 ...
4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27). *Category:EC 1.13.12 (With incorporation of one atom of oxygen (internal ... Catechol-O-methyl transferase EC 2.1.1.6. *DNA methyltransferase EC 2.1.1.72, EC 2.1.1.113, EC 2.1.1.37 ... Category:EC 1.10.1(with NAD+ or NADP+ as acceptor). *Category:EC 1.10.2 (with a cytochrome as acceptor) *Coenzyme Q - ... Category:EC 1.3.2 (with a cytochrome as acceptor). *Category:EC 1.3.3 (with oxygen as acceptor) *Protoporphyrinogen oxidase EC ...
The meta-hydroxyl group of catechol is methylated by catechol-O-methyltransferase. Four of the five hydroxyl groups of ... 3-dioxygenase. *Quercetin 3-O-methyltransferase. *Quercetin-3-sulfate 3'-sulfotransferase ... 2 (5): 235-44. doi:10.1039/c1fo10025d. PMC 4122511. PMID 21779561.. *^ a b Day AJ, Rothwell JA, Morgan R (2004). " ... Gross P (March 1, 2009), New Roles for Polyphenols. A 3-Part Report on Current Regulations & the State of Science, ...
... can be directly metabolized by catechol-O-methyl transferase to 3-O-methyldopa, and then further to vanillactic acid. ... 4-Hydroxyphenylpyruvate dioxygenase. *Homogentisate 1,2-dioxygenase. *Fumarylacetoacetate hydrolase. *tyrosine→melanin: ... 171 (2): 403-14. doi:10.1111/bph.12459.. *^ Jürgen Martens, Kurt Günther, Maren Schickedanz: "Resolution of Optical Isomers by ... 2 (3): 126-133.. *^ Waite, J. Herbert; Andersen, Niels Holten; et al. (2005). "Mussel Adhesion: Finding the Tricks Worth ...
Tetralin-Induced and ThnR-Regulated Aldehyde Dehydrogenase and β-Oxidation Genes in Sphingomonas macrogolitabida Strain TFAIn the absence of a suitable substrate for the dioxygenase, ThnA3 would accumulate in its reduced form and act as a negative ... is responsible for the extradiol cleavage of the catechol derivative (1), followed by hydrolytic cleavage (by ThnD) of the C-C ... Identification of an extradiol dioxygenase involved in tetralin biodegradation: gene sequence analysis, purification, and ... Biotechnol. 2:262-273. [PubMed]. 37. Toth, J., A. A. Ismaiel, and J.-S. Chen. 1999. The ald gene, encoding a coenzyme A- ...
pubmedcentralcanada.ca
The regulation of catabolic pathway of aromatic compound degradation in Acinetobacter radioresistens: physiological and...Benzoate Dioxygenase, Catechol 1,2-Dioxygenase). These results allow us to have a global picture of the complex regulation ... Benzoate Dioxygenase, Catechol 1,2-Dioxygenase). These results allow us to have a global picture of the complex regulation ... 0.520 h-1, Y= 10,1 g/Cmol). Furthermore bacteria were grown in presence of substrate mixtures or utilizing cross-acclimatised ... 0.520 h-1, Y= 10,1 g/Cmol). Furthermore bacteria were grown in presence of substrate mixtures or utilizing cross-acclimatised ...
国際学会3-hydroxyanthranilate 3,4-dioxygenase, a novel eukaryotic homolog in the degradation pathway of 2-nitrobenzoate by ... Metabolite analyses by high-performance liquid chromatograph indicated that 3-hydroxyanthranilate, anthranilate, and catechol ... Lett.327(2),142-1472012年1月 学会発表海洋性フタル酸資化性菌の分離と解析査読無その他共著西村彩香（D）;岩木宏明;長谷川喜衛;;日本防菌防黴学会 第
Pascal, R; Huang, O (1986). "Reactions of 3-ethylcatechol and 3-(methylthio)catechol with catechol dioxygenases". Arch. Biochem ... a family of catechol dioxygenases that cleaves the bond between the phenolic hydroxyl groups of catechol using an Fe3+ cofactor ... Two families of dioxygenases were discovered by Osamu Hayaishi and Kizo Hashimoto in 1950: catechol 1,2-dioxygenase and ... catechol dioxygenase, pyrocatechase, pyrocatechol 1,2-dioxygenase, CD I, CD II) is an enzyme that catalyzes the oxidative ring ...
Sequence of the plasmid-encoded catechol 1,2-dioxygenase-expressing gene, pheB, of phenol-degrading Pseudomonas sp. strain...Phenol monooxygenase (PMO) and catechol 1,2-dioxygenase (C12O), the two first enzymes of the phenol-degradation pathways, are ... Sequence of the plasmid-encoded catechol 1,2-dioxygenase-expressing gene, pheB, of phenol-degrading Pseudomonas sp. strain ... 1. Laboratory of Plasmid Biology, Estonian Biocenter, Tartu, U.S.S.R.. ... Kivisaar M1, Kasak L, Nurk A.. Author information. ...
RCSB PDB - 3I4Y: Crystal structure determination of Catechol 1,2-Dioxygenase from Rhodococcus opacus 1CP in complex with 3,5...Crystal structure determination of Catechol 1,2-Dioxygenase from Rhodococcus opacus 1CP in complex with 3,5-dichlorocatechol ... ion containing enzyme specialized in the aerobic biodegradation of catechols, and its adducts with catechol, 3-methylcatechol, ... Catechol 1,2-dioxygenase from the Gram-positive Rhodococcus opacus 1CP: Quantitative structure/activity relationship and the ... The structural analyses reveal the diverse modes of binding to the active metal iron ion of the tested catechols thus allowing ...
Catechol 1,2-dioxygenase from α-naphthol degrading thermophilic Geobacillus sp. strain: purification and properties - Semantic...G27 strain catechol 1,2-dioxygenase were determined. The enzyme had apparent Km of 29 µM for catechol and the cleavage ... The catechol 1,2-dioxygenase (C1,2O) was purified using four steps of ammonium sulfate precipitation, DEAE-celullose, Sephadex ... A half-life of the catechol 1,2-dioxygenase at the optimum temperature was 40 min. The kinetic parameters of the Geobacillus sp ... The enzyme was purified about 18-fold with a specific activity of 7.42 U mg of protein−1. The relative molecular mass of the ...
Engineering catechol 1, 2-dioxygenase by design for improving the performance of the cis, cis-muconic acid synthetic pathway in ... Rights & permissionsfor article Engineering catechol 1, 2-dioxygenase by design for improving the performance of the ,i,cis, ... LTP-1, a novel antimitotic agent and Stat3 inhibitor, inhibits human pancreatic carcinomas in vitro and in vivo *Han-Li Huang ... 2 September 2016 , Open Rapid and cyclic dust accumulation during MIS 2 in Central Asia inferred from loess OSL dating and ...
Kobayashi S, Hayaishi O (1970). "Anthranilic acid conversion to catechol (Pseudomonas)". Methods Enzymol. 17A: 505-510. doi: ... catechol + CO2 + NAD(P)+ + NH3 The 5 substrates of this enzyme are anthranilate, NADH, NADPH, H+, and O2, whereas its 5 ... "Enzymatic formation of catechol from anthranilic acid". J. Biol. Chem. 239: 2204-2211. PMID 14209949. Molecular and Cellular ... products are catechol, CO2, NAD+, NADP+, and NH3. This enzyme belongs to the family of oxidoreductases, specifically those ...
DE SubName: Full=Catechol dioxygenase, putative (AFU_orthologue AFUA_2G02910) {ECO:0000313,EMBL:CBF84491.1}; GN ORFNames=ANIA_ ... DR Pfam; PF00775; Dioxygenase_C; 1. DR Pfam; PF04444; Dioxygenase_N; 1. DR SUPFAM; SSF49482; SSF49482; 1. PE 4: Predicted; DR ... DR GO; GO:0018576; F:catechol 1,2-dioxygenase activity; IEA:InterPro. DR GO; GO:0008199; F:ferric iron binding; IEA:InterPro. ... DR GO; GO:0009712; P:catechol-containing compound metabolic process; IEA:InterPro. DR Gene3D; 2.60.130.10; -; 1. DR InterPro; ...
Veronika Kremlackova - NeL.eduIsolation and partial characterization of catechol 1,2-dioxygenase of phenol degrading yeast Candida tropicalis.. Vilimkova L, ... Isolation and partial characterization of extracellular NADPH-dependent phenol hydroxylase oxidizing phenol to catechol in ... Catechols:chemistry, Chromatography, Chromatography, Gel, Chromatography, High Pressure Liquid, Cytosol:chemistry, Dextrans, ... Catechols:metabolism, Chromatography, High Pressure Liquid, Cloning, Molecular, Comamonas testosteroni:enzymology, ...
Category:Acinetobacter - Wikimedia CommonsStructure of Catechol 1,2-dioxygenase from Acinetobacter Calcoaceticus Native Data.jpg 500 × 500; 48 KB. ...
EAWAG-BBD: reaction, reacID# r1207... catechol , / 1,2-dioxygenase ,/ 1.13.11.1 , Search GenBank, 769 hits on Apr. 03, 2012 Kyoto ,\ ExPASy , \ , 2H+ v 3-Fluoro-cis, ... Appl Environ Microbiol (1980) 39(1): 58-67. Search Medline for 4-fluorocatechol metabolism AND bacteria. 17 citations on ...
Search Medline for catechol 1,2-dioxygenase AND 4-chlorocatechol. 36 citations on March 09, 2012. 4-Chlorocatechol , , O2 ... catechol , / 1,2-dioxygenase ,/ 1.13.11.1 , Search GenBank, 769 hits on Apr. 03, 2012 Kyoto ,\ ExPASy , \ , 2H+ v 3-Chloro-cis, ... 2,4-Dichlorophenoxyacetic Acid] [BBD Main Menu] Page Author(s): Brian Hill July 03, 2003 Contact Us This is the EAWAG-BBD ...
EC 1.13.113-dioxygenase. misc/catechol Reaction: catechol + O2 = 2-hydroxymuconate-6-semialdehyde. For diagram of reaction click here.. ... 3-dioxygenase. EC 1.13.11.18 sulfur dioxygenase. EC 1.13.11.19 cysteamine dioxygenase. EC 1.13.11.20 cysteine dioxygenase. EC ... EC 1.13.11.87 endo-cleaving rubber dioxygenase. EC 1.13.11.1. Accepted name: catechol 1,2-dioxygenase. Reaction: catechol + O2 ... A novel non-heme iron-containing dioxygenase. Chloridazon-catechol dioxygenase from Phenylobacterium immobilis DSM 1986. Eur. J ...
3-dioxygenase, encoded by ntdAaAbAcAd. The catechol degradation (Ctd) enzymes were identified based on the analysis of the JS42 ... catechol 2,3-dioxygenase; CtdF, 2-hydroxymuconate semialdehyde hydrolase; CtdJ, 2-oxopent-4-dienoate hydratase; CtdK, 4-hydroxy ... strain JS42 to 2-nitrotoluene.. Rabinovitch-Deere CA1, Parales RE.. Author information. 1. Department of Microbiology, College ... Fig 2. Wild-type JS42m responses in the qualitative capillary assay. Responses to buffer (negative control) or succinate (10 mM ...
Frontiers | Metabolic Engineering of the Shikimate Pathway for Production of Aromatics and Derived Compounds-Present and Future...It was shown that in E. coli PCA and catechol can be produced this way, with the highest yield being that of catechol at a ... Thompson, B. (2017). Engineering Escherichia coli for the Novel and Enhanced Biosynthesis of Phenol, Catechol, and Muconic Acid ... It has been proposed that this precursor is rather 4-hydroxyphenylpyruvate then catechol, giving rise to a second pathway to ... and catechol by means of the aroZ gene from Klebsiella pneumoniae encoding DHS dehydratase, the aroY gene from K. pneumoniae ...
... the crystal structure of catechol 2,3-dioxygenase (metapyrocatechase) from Ppseudomonas putida mt-2. ... STRUCTURE OF CATECHOL 2,3-DIOXYGENASE (METAPYROCATECHASE) FROM PSEUDOMONAS PUTIDA MT-2. ...
Different Spectra of Stationary-Phase Mutations in Early-Arising versus Late-Arising Mutants of Pseudomonas putida: Involvement...Sequence of the plasmid-encoded catechol 1,2-dioxygenase-expressing gene, pheB, of phenol-degrading Pseudomonas sp. strain EST ... Transformants were assayed for expression of the pheB gene by testing expression of catechol 1,2-dioxygenase as described ... The pheBA genes encode catechol 1,2-dioxygenase and phenol monooxygenase, respectively (33). When the pheBA-expressing plasmid ... 1. Accumulation of Phe+ mutants on phenol minimal plates in P. putida wild-type strain PaW85 (WT) and its RpoS-defective ...
Iron(III) complexes with a tripodal N3O ligand containing an internal base as a model for catechol intradiol-cleaving...... complexes with a tripodal N3O ligand containing an internal base as a model for catechol intradiol-cleaving dioxygenases. Li, ... protocatechuate 3,4-dioxygenase as an internal base. The spectrop h oto metric titration experiment indicates the relatively ... reactive functional-model, nonheme iron enzymes, protocatechuate 3,4-dioxygenase, crystal-structure, active-sites, (catecholato ... and the coordinate ethoxyl arm of the ligand is capable of accepting the proton from catechol, which mimics the function of ...
Utilization of aromatic compounds as carbon and energy sources during growth and N2-fixation by free-living nitrogen fixing...Aromatic catabolism Free-living diazotrophs Nitrogen fixation catechol dioxygenases Protocatechuate dioxygenases A preliminary ... All six species growing on substrates typically converted to catechol expressed inducible catechol 1,2-dioxygenase and/or ... 5-dioxygenase was expressed. A. chroococcum expressed only ortho cleavage dioxygenases during growth on naphthalene and 4- ... toluate and only meta cleavage dioxygenases on the other aromatics. B. mobilis expressed only ortho cleavage dioxygenases. The ...
Simultaneous Determination of Catechol, Resorcinol and Hydroquinone with Kalman Filtering Ultraviolet Spectrophotometry--...... catechol(1,2-dihydroxybenzene), resorcinol(1,3-dihydroxybenzene and hydroquinone(1,4-dihydroxybenzene) without preliminary ... 273nm and 288mp respectively.The average recoveries of catechol, resorcinol and hydroquinone in standard mixtures are 97.4%, ... Simultaneous Determination of Catechol, Resorcinol and Hydroquinone with Kalman Filtering Ultraviolet Spectrophotometry. ... Simultaneous determination of catechol and hydroquinone in graphene modified electrode[J];武汉工程大学学报;2013-02. ...
Breed KMRh Strain Passport - StrainInfoRhodococcus rhodochrous catechol 1,2-dioxygenase (catA) gene, partial cds. DSM 43241 T ... Applicability of the functional gene catechol 1,2-dioxygenase as a biomarker in the detection of BTEX-degrading Rhodococcus ... 33 items found, displaying 1 to 25.[First/Prev] 1, 2 [Next/Last] ... 33 items found, displaying 1 to 25.[First/Prev] 1, 2 [Next/Last ... Proc Natl Acad Sci U S A 101(1), 314-319, 2004 ...
A multidisciplinary characterization of catechol 1,2 dioxygenase. Biochimica et Biophysica Acta, 1814(6), 817-823.Google ... Indian Journal of Pharmaceutical Sciences, 74(1), 1-17.PubMedPubMedCentralGoogle Scholar ... Journal of Laboratory Automation, 18(1), 19-29.PubMedPubMedCentralGoogle Scholar ... Expert Review of Proteomics, 8(1), 43-59.PubMedPubMedCentralGoogle Scholar ...
624d) Mass Balance Based Semi-Quantification of Adipic Acid Producing Enzyme Cascade | AIChETo produce adipic acid in Escherichia coli, we utilize an enzyme cascade of catechol 1,2-dioxygenase (CatA) and enoate ... One common pathway for microbial production of adipic acid goes through a catechol intermediate to form muconic acid, which is ... To quantify metabolism of different substituted catechols, we developed a semi-quantification method based on the conserved ... Furthermore, we characterize our enzyme cascade by analyzing its metabolism of different substituted catechols available from ...
Phenol hydroxylaseEnzymes11.1EnzymeBiodegradationSubstratesC23OPathwayAromaticGeneGentisate 1,2-dioxygenaseChlorocatecholPurification and characterizationOrtho-cleavageBenzoate DioxygenaseHydroxyquinolFunction of dioxygenasesEscherichiaPathwaysEncodesExtradiol dioxygenasePyrogallolMetabolismCatalysesMetabolicRhodococcusMuconic acidReactionsNitrogenReductaseMicrobiolMicrobiologyOrganismCatalytic mechanismSequenceDerivativesPseudomonad
- The study was finally completed by a transcriptome analysis of the most relevant catabolic genes (Phenol Hydroxylase, Benzoate Dioxygenase, Catechol 1,2-Dioxygenase). (unito.it)
- Moreover the high structural homology of the present enzyme with the 3-chlorocatechol 1,2-dioxygenase from the same bacterium are discussed in terms of their different substrate specificity. (rcsb.org)
- The enzyme was purified about 18-fold with a specific activity of 7.42 U mg of protein−1. (semanticscholar.org)
- The systematic name of this enzyme class is anthranilate,NAD(P)H:oxygen oxidoreductase (1,2-hydroxylating, deaminating, decarboxylating). (wikipedia.org)
- The Mn sites mimic the manganese-dependent dioxygenase (MndD), which is an enzyme that catalyses the oxidation of catechol derivatives. (rsc.org)
- To produce adipic acid in Escherichia coli, we utilize an enzyme cascade of catechol 1,2-dioxygenase (CatA) and enoate reductase (ER). (aiche.org)
- Furthermore, we characterize our enzyme cascade by analyzing its metabolism of different substituted catechols available from lignin pyrolysis. (aiche.org)
- This method allowed us to quantify compounds via liquid chromatography-mass spectroscopy that do not have commercially available standards, and compare conversion of substituted catechols by our adipic acid-producing enzyme cascade. (aiche.org)
- For the elucidation of the active site structure of another well characterized oxygenase, catechol 2, 3 - dioxygense, we used o-nitrophenol, a competitive inhibitor, and found it a useful active site probe for this enzyme. (nii.ac.jp)
- The structural and spectroscopic characterization of mononuclear iron(III)-catecholato complexes of ligand L4 (methyl bis(1-methylimidazol-2-yl)(2-hydroxyphenyl)methyl ether, HL4) are described, which closely mimic the enzyme-substrate complex of the intradiol-cleaving catechol dioxygenases. (uu.nl)
- The enzyme catalyses the conversion of a colourless substrate (catechol or substituted catechols) into a bright yellow product (2-hydroxymuconic semialdehyde) within seconds of substrate addition. (igem.org)
- GFP is N-terminally added to Catechol-2,3-dioxygenase enzyme so that it does not allows formation of the tetramer due to a steric hindrance effect. (igem.org)
- Tetramerisation of the monomers produces the active C23O enzyme, which in the presence of catechol substrate produces a yellow colour. (igem.org)
- Protocatechuate 3,4-dioxygenase (EC 1.13.11.3 ) (3,4-PCD), an oligomeric enzyme complex which consists of 12 copies each of an α and a β subunits. (expasy.org)
- The catechol 2,3-dioxygenase (C23O) enzyme of this pathway catalyses the conversion of catechol to 2-hydroxymuconic semialdehyde. (diva-portal.org)
- 2002). Analysis of the purified E. coli enzyme showed (1) no copurifying prosthetic group detectable by spectroscopy and (2) no inhibition of enzyme activity by heme in vitro. (phosphorylase-signal.com)
- This hypothesis was supported by the results from enzyme assays of the crude cell extract of the consortium revealing catechol 1,2-dioxygenase activity which converted catechol and 4-chlorocatechol to cis,cis-muconic acid and 3-chloro-cis,cis-muconic acid respectively. (kent.ac.uk)
- However, the enzyme had a much higher conversion rate for catechol [156 U (g protein)(-1)] than for 4-chlorocatechol [17.2 U (g protein)(-1)], indicating preference for non-chlorinated substrates. (kent.ac.uk)
- The enzyme forms a cis- dihydroxylated product that spontaneously rearranges to form a catechol with accompanying release of nitrite. (qmul.ac.uk)
- Moreover, in the presence of naproxen activity of gentisate 1,2-dioxygenase, enzyme engaged in 4-hydroxybenzoate metabolism, was completely inhibited. (pjmonline.org)
- 1,2,3-Trichloropropane (TCP) is a toxic compound that is recalcitrant to biodegradation in the environment. (asm.org)
- A potential biodegradation pathway starts with hydrolytic dehalogenation to 2,3-dichloro-1-propanol (DCP), followed by oxidative metabolism. (asm.org)
- The genome sequence of R.pyridinivorans strain AK37 has identified several key enzymes involved in the six pathways of monocyclic aromatic compound biodegradation: protocatechuate 3,4-dioxygenase, benzoate 1,2-dioxygenase, 3-ketosteroid-9α-hydroxylase, 3-ketosteroid-σ-dehydrogenase, and so on. (kenyon.edu)
- Biodegradation has long been seen as a cost-effective and ecological way to eliminate environmental contamination [ 2 ]. (hindawi.com)
- Biodegradation of tetralin by the strain TFA involves initial oxidation of the aromatic ring to yield 1,2-dihydroxytetralin (1,2-DHT) through reactions catalyzed by a ring-hydroxylating dioxygenase and by a dehydrogenase ( 37 ). (asm.org)
- The GC-MS data also shows the presence of catechol and 2-hydroxymuconic semialdehyde , which confirms the established pathway of benzene biodegradation. (thefreedictionary.com)
- International Journal of Environmental Bioremediation & Biodegradation , 2014 2 (2), pp 75-83. (sciepub.com)
- To investigate the characteristic and biochemical mechanism about the phenol biodegradation by bacterial strains ZD 4-1 and ZD 4-3. (bvsalud.org)
- A. Nardi, R. Avrahami, E. Zussman, J. Rokem and C. Greenblatt, "Phenol Biodegradation by Corynebacterium glutamicum Encapsulated in Electrospun Fibers," Journal of Environmental Protection , Vol. 3 No. 2, 2012, pp. 164-168. (scirp.org)
- The structural analyses reveal the diverse modes of binding to the active metal iron ion of the tested catechols thus allowing to identify the residues selectively involved in recognition of the diverse substrates by this class of enzymes. (rcsb.org)
- The catalytic rates for Rho 1,2-CTD conversion of the tested compounds are also compared with the calculated energies of the highest occupied molecular orbital (E(HOMO)) of the substrates. (rcsb.org)
- All six species growing on substrates typically converted to catechol expressed inducible catechol 1,2-dioxygenase and/or catechol 2,3-dioxygenase. (springer.com)
- When grown on substrates typically converted to protocatechuate, inducible protocatechuate 3,4-dioxygenase and/or protocatechuate 4,5-dioxygenase was expressed. (springer.com)
- Dioxygenases catalyze the incorporation of both atoms of molecular oxygen into substrates. (expasy.org)
- The substrates of ring-cleavage dioxygenases can be classified into two groups according to the mode of scission of the aromatic ring. (expasy.org)
- Other catechols are also substrates [J (ormedmedical.us)
- Catechol, 3- and 4-methylcatechol are poor substrates [G (ormedmedical.us)
- Catechol (2,3) dioxygenase (C23O) is the protein product of the XylE gene. (igem.org)
- lapB , the first gene in the lap operon, encodes catechol 2,3-dioxygenase (C23O). (microbiologyresearch.org)
- C23O preferentially oxidizes 4-alkylcatechols via proximal (2,3) cleavage. (microbiologyresearch.org)
- 2-Nitrotoluene (2NT) degradation pathway in Acidovorax sp. (nih.gov)
- The occurrence of 2- to 3-bp deletions required the stationary-phase sigma factor RpoS, which indicates that some mutagenic pathway is positively controlled by RpoS in P. putida . (asm.org)
- One common pathway for microbial production of adipic acid goes through a catechol intermediate to form muconic acid, which is then reduced to form adipic acid. (aiche.org)
- A previously developed genome-scale reconstruction of E. coli (iJR904) was altered to include a catechol transport reaction and a reaction representing the breakdown of catechol via the pathway specified in our design. (igem.org)
- The catechol pathway that is inserted into E. coli is shown below. (igem.org)
- The inserted degradation pathway breaks down catechol into acetyl-CoA, which plays a central role in biochemical networks by serving as a connecting hub for many metabolic pathways. (igem.org)
- THe pathway of aromatic compound degradation by strain AK 37 has not been described ( 1 ). (kenyon.edu)
- The lower pathway includes cleavage of the aromatic ring by dioxygenases with formation of (chloro)maleylacetates. (asmscience.org)
- As shown in Fig. Fig.1A, 1A , strain TFA metabolizes tetralin through a meta -cleavage pathway that involves an initial dioxygenation step (catalyzed by the enzymatic complex ThnA1A2A3A4), followed by a dehydrogenation step (catalyzed by ThnB) to produce 1,2-dihydroxytetralin ( 24 ). (pubmedcentralcanada.ca)
- The data consistently indicated that induction of the ortho pathway by benzoate plasmid-less strain P. putida KT2440 led to catechol surplus, the toxicity of which at high concentrations being counteracted by CatA2. (csic.es)
- Addition of bipyridyl to incubated medium resulted in accumulation of terminal aromatic compound, suggesting that catechol may be terminal aromatic compound in degradation pathway of H-acid by A. latus. (iisc.ernet.in)
- Product accumulation studies identified (4R)-carvone, (1R,4R)-dihydrocarvone, (4R,7R)-4-isopropenyl-7-methyl-2-oxo-oxepanone, (3R)-6-hydroxy-3-isopropenylheptanoate, (3R)-3-isopropenyl -6-oxoheptanoate, (3S,6R)-6-isopropenyl-3-methyl-2-oxo-oxepanone and (5R)-6-hydroxy-5-isopropenyl-2-methylhexanoate as intermediates in the (4R)carveol degradation pathway. (tudelft.nl)
- Two key metabolites of the meta-cleavage pathway, 5-formyl-2-hydroxy-6,6,8,8-tetramethylnona-2,4-dienoic acid and 6,6,8,8-tetramethyl-2-oxonon-4-enoic acid, were detected by LC-ESI-Q-TOF. (ncu.edu.tw)
- No activity was detected for catechol 1,2-dioxygenase, indicating the presence of a meta cleavage pathway of toluene degradation, typical of that found on TOL type plasmids, and not ortho cleavage of catechol typical of chromosomal gene expression. (waikato.ac.nz)
- This degradative mechanism is analogous to the toluene degradative pathway encoded by the archetypal TOL plasmid, pWWO, in the mesophilic bacterium P. putida mt-2. (waikato.ac.nz)
- stress PS12: an entire mineralization pathway seems to have advanced through transposon-mediated recruitment of toluene or benzene dioxygenase and dihydrodiol dehydrogenase genes following to a gene cluster coding for an gene cluster, hereafter known as the gene cluster) in the trichlorobenzene-degrading stress sp. (globaltechbiz.com)
- These bacteria subsequently employ 1,2-CTD in the last step of the degradation of aromatic compounds to aliphatic products. (wikipedia.org)
- Six species of free-living nitrogen fixing bacteria, Azomonas agilis, Azospirillum brasilense, Azospirillum lipoferum, Azotobacter chroococcum, Azotobacter vinelandii , and Beijerinckia mobilis , were surveyed for their ability to grow and fix N 2 using aromatic compounds as sole carbon and energy source. (springer.com)
- The organic solvent tetralin (1,2,3,4-tetrahydronaphthalene) is a bicyclic molecule composed of an aromatic and an alicyclic moiety, which share two carbon atoms. (asm.org)
- Cleavage of aromatic rings is one of the most important function of dioxygenases. (expasy.org)
- These events, apparently confined in proximity to 1 to 2 kb (the length of a typical gene) by the length of a replication fork, appear to be RecA independent ( 9 , 31 ), in contrast to large sequence-guided mutations which depend upon RecA-mediated recombination. (asm.org)
- An important gene associated with Mycobacterium Fortuitum is HSPD1 (Heat Shock Protein Family D (Hsp60) Member 1), and among its related pathways/superpathways are Sudden Infant Death Syndrome (SIDS) Susceptibility Pathways and Cytokine production by Th17 cells in CF (Mouse model) . (malacards.org)
- P. putida mt-2 has a second chromosomal copy of the catA gene (named catA2) located downstream of the ben operon that encodes an additional catechol-1,2-dioxygenase. (csic.es)
- Purification and characterization of catechol 1,2-dioxygenase from Rhodococcus rhodochrous NCIMB 13259 and cloning and sequencing of its catA gene. (cbrc.jp)
- A total of 11,195 protein-coding genes were predicted including a diverse group of gene families involved in hydrocarbon degradation pathways like dioxygenases and cytochrome P450. (biomedcentral.com)
- 6. The composition of claim 1 wherein the reporter gene comprises lacZ, bgaB, xylE, cat, gfp, or a mixture of two or more thereof. (patents.com)
- The gene cluster to KT2442 confers on this strain the ability to ROBO4 grow on 3-chlorobenzoate (3-CBA) (41), and (iii) the chlorocatechol 1,2-dioxygenase TcbC possesses the ability to cleave di- and tri- as well as monochlorinated catechols (39), potentially permitting the mineralization of multiply chlorinated aromatics. (globaltechbiz.com)
- FIG. 1 (A) Diagram of pBJ44 illustrating the inner (I) and outer (O) minitransposon ends, the gene cluster (KT2442, a strain capable of growth on benzoate. (globaltechbiz.com)
- 2014). After growth of strain KB2 on naproxen activity of phenol monooxygenase, naphthalene dioxygenase, hydroxyquinol 1,2-dioxygenase and gentisate 1,2-dioxygenase was observed. (pjmonline.org)
- A quantitative relationship (R=0.966) between the ln k(cat) and the calculated electronic parameter E(HOMO) was obtained for catechol, 3-methylcatechol, 4-methylcatechol, pyrogallol, 3-chlorocatechol, 4-chlorocatechol. (rcsb.org)
- Preferentially converts 3-chlorocatechol and 3,5-dichlorocatechol as opposed to other chlorinated catechols. (rcsb.org)
- Search Medline for catechol 1,2-dioxygenase AND 4-chlorocatechol . (ethz.ch)
- The purpose of this study was purification and characterization of catechol 1,2-dioxygenase from Geobacillus sp. (semanticscholar.org)
- A. chroococcum expressed only ortho cleavage dioxygenases during growth on naphthalene and 4-toluate and only meta cleavage dioxygenases on the other aromatics. (springer.com)
- B. mobilis expressed only ortho cleavage dioxygenases. (springer.com)
- KT2442 is able to convert 3-CBA to 3- and 4-chlorocatechols, presumably through broad-spectrum benzoate dioxygenase and dihydrodiol dehydrogenase enzymes inside a fashion related to that observed with sp. (globaltechbiz.com)
- It was shown that the activity of monooxygenase, hydroxyquinol 1,2-dioxygenase, protocatechuate 3,4-dioxygenase and protocatechuate 4,5-dioxyegnase in strain S5 was induced after growth of the strain with naproxen and 4-hydroxybenzoate. (pjmonline.org)
- The obtained results suggest that monooxygenase and hydroxyquinol 1,2-dioxygenase are the main enzymes in naproxen degradation by Planococcus sp. (pjmonline.org)
- Structure and Function of Dioxygenases. (umn.edu)
- To quantify metabolism of different substituted catechols, we developed a semi-quantification method based on the conserved mass balance within our Escherichia coli system. (aiche.org)
- Biosynthesis of catechol melanin from glycerol employing metabolically engineered Escherichia coli Microbial Cell Factories, 15, 161. (unam.mx)
- Producci n de un anticuerpo humano capaz de neutralizar toxinas de alacranes mexicanos en Escherichia coli. (unam.mx)
- Expresi n de la prote na recombinante Mgfp-5 de Mytilus galloprovincialis en Escherichia coli. (unam.mx)
- These data reveal the role of CatA2 as a type of metabolic safety valve for excess catechol that alleviates the metabolic conflict generated by simultaneous expression of the meta and ortho pathways, thereby facilitating their co-existence. (csic.es)
- To address the decontamination of (1) toxic organic compounds such as naphthene and catechol found in the tailings ponds, natural pathways that can break down the toxic chemicals can be utilized. (igem.org)
- The catechol intermediate is further metabolized through reactions catalyzed by an extradiol dioxygenase, a hydrolase, a hydratase, and an aldolase, respectively ( 3 , 24 , 25 ). (asm.org)
- Cleavage of pyrogallol by non-heme iron-containing dioxygenases. (cbrc.jp)
- 6 This is the case of catechol dioxygenase, which catalyses the cleavage of catechol derivatives. (rsc.org)
- Though experimentally determined transport rates of these energy sources are not yet available, we will do a preliminary analysis of the response to catechol uptake by investigating different points on the predicted metabolic landscape. (igem.org)
- The predicted optimal uptake of catechol through FBA of the E. coli metabolic model is 2.52 mmol/gDW/h, resulting in a growth rate of 0.744 h-1. (igem.org)
- This is a prediction of the optimal catechol input into the metabolic system in order to produce the maximum amount of biomass components, taking into account the steady-state balancing of all associated metabolic compounds and cofactors. (igem.org)
- Though prediction of metabolic flux and microbial growth rate has been shown to be generally accurate through this method, experimental usage of catechol may be significantly higher or lower than the purely stoichiometric prediction. (igem.org)
- This is because the input of catechol into the metabolic network in vivo may trigger metabolic regulation at the expense of the assumed cellular objective (biomass production). (igem.org)
- Furthermore, even at a given combination of glucose and catechol uptake rate, there is a degree of variability associated with the in silico prediction of internal metabolic fluxes. (igem.org)
- 1. Investigation of the research on the metabolic regulation in China : A five member delegation from our University visited Changchun city and Jilin city in China in August 1990, and delivered lectures at Central Hospital of Changchun city and Jilin Medical College. (nii.ac.jp)
- In 2010, the Lethbridge iGEM team confirmed that xylE when introduce to E. coli hydrolyzes catechol to 2-HMS demonstrating and that this critical step can successfully be performed to convert toxic chemicals to metabolic intermediates 7 . (igem.org)
- Although the genus, Rhodococcus, implies the coccus shape of the organisms, the bacteria surprisingly show extensive polymorphism (Fig. 1. (kenyon.edu)
- 1,2-CTD uses Fe3+ as a cofactor to cleave the carbon-carbon bond between the phenolic hydroxyl groups of catechol, thus yielding muconic acid as its product. (wikipedia.org)
- Reactions of 3-ethylcatechol and 3-(methylthio)catechol with catechol dioxygenases. (cbrc.jp)
- All heteroatoms (N3O) of the ligand are coordinated to the iron center in complex 1 with two pyridine nitrogen atoms on the axial bonds, while one of the pyridyl arms of the ligand is left uncoordinated in complex 2. (diva-portal.org)
- ThnY is similar to the ferredoxin reductase components of dioxygenase systems and shows the fer2 domain, binding a Cys 4 [2Fe-2S] iron sulfur center, and the FAD-binding domain, common to those reductases. (asm.org)
- Frontiers in Microbiology, 9, 1−19.10.3389/fmicb.2018.00015. (etis.ee)
- In: Applied and Environmental Microbiology, 60 (1). (iisc.ernet.in)
- In: Current Microbiology, 20 (2). (iisc.ernet.in)
- In: Applied and Environmental Microbiology, 56 (1). (iisc.ernet.in)
- FEMS Microbiology Letters, 366 (1). (csircentral.net)
- S. Thomas, S. Sarfaraz, L. C. Mishra and L. Iyengar "Degradation of Phenol and Phenolic Compounds by a Defined Denitrifying Bacterial Culture," World Journal of Microbiology, Vol. 18, No. 1, 2002, pp. 57-63. (scirp.org)
- Because of its extraordinary position as a preferred model in biochemical genetics, molecular biology, and biotechnology, E. coli K-12 was the earliest organism to be suggested as a candidate for whole genome sequencing ( 1 , 2 ). (sciencemag.org)
- 3. The composition of claim 1 wherein the host organism comprises bacteria of the Bacillus or Clostridia genera. (patents.com)
- 5. The composition of claim 1 wherein the host organism comprises Geobacillus stearothermophilus. (patents.com)
- The catalytic mechanism of catechol 1,2-dioxygenase was elucidated using a combination of O18 labeling experiments and crystallography. (wikipedia.org)
- 6-9 In particular, the manganese-dependent dioxygenase (MndD) is a system that has attracted much attention since its catalytic mechanism is still under discussion. (rsc.org)
- DT 26-APR-2005, sequence version 1. (univ-lyon1.fr)
- Bacterial strains ZD 4-1 and ZD 4-3 were isolated by using phenol as the sole source of carbon and energy, and identified by 16S rDNA sequence analysis. (bvsalud.org)
- Nitrobenzene and its derivatives (NBDs) are a group of toxic chemicals that are widespread in the environment owing to their worldwide use [ 1 ]. (hindawi.com)
- Two catechol 1,2-dioxygenases from a 3-chlorobenzoate-grown pseudomonad. (naver.com)