Process by which micro-organisms adapt quickly to a preferred rapidly-metabolizable intermediate through the inhibition or repression of genes related to CATABOLISM of less preferred source(s).
The interference in synthesis of an enzyme due to the elevated level of an effector substance, usually a metabolite, whose presence would cause depression of the gene responsible for enzyme synthesis.
A family of galactoside hydrolases that hydrolyze compounds with an O-galactosyl linkage. EC 3.2.1.-.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
The bacterial sugar phosphotransferase system (PTS) that catalyzes the transfer of the phosphoryl group from phosphoenolpyruvate to its sugar substrates (the PTS sugars) concomitant with the translocation of these sugars across the bacterial membrane. The phosphorylation of a given sugar requires four proteins, two general proteins, Enzyme I and HPr and a pair of sugar-specific proteins designated as the Enzyme II complex. The PTS has also been implicated in the induction of synthesis of some catabolic enzyme systems required for the utilization of sugars that are not substrates of the PTS as well as the regulation of the activity of ADENYLYL CYCLASES. EC 2.7.1.-.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.
A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.
Proteins found in any species of bacterium.
A species of gram-positive bacteria that is a common soil and water saprophyte.
A transcriptional regulator in prokaryotes which, when activated by binding cyclic AMP, acts at several promoters. Cyclic AMP receptor protein was originally identified as a catabolite gene activator protein. It was subsequently shown to regulate several functions unrelated to catabolism, and to be both a negative and a positive regulator of transcription. Cell surface cyclic AMP receptors are not included (CYCLIC AMP RECEPTORS), nor are the eukaryotic cytoplasmic cyclic AMP receptor proteins, which are the regulatory subunits of CYCLIC AMP-DEPENDENT PROTEIN KINASES.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Derivatives of SUCCINIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a 1,4-carboxy terminated aliphatic structure.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
An enzyme that catalyzes the conversion of L-tryptophan and water to indole, pyruvate, and ammonia. It is a pyridoxal-phosphate protein, requiring K+. It also catalyzes 2,3-elimination and beta-replacement reactions of some indole-substituted tryptophan analogs of L-cysteine, L-serine, and other 3-substituted amino acids. (From Enzyme Nomenclature, 1992) EC 4.1.99.1.
An enzyme that catalyzes the first step of histidine catabolism, forming UROCANIC ACID and AMMONIA from HISTIDINE. Deficiency of this enzyme is associated with elevated levels of serum histidine and is called histidinemia (AMINO ACID METABOLISM, INBORN ERRORS).
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A urea hydantoin that is found in URINE and PLANTS and is used in dermatological preparations.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.
A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.
A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, and sweetening agent.
Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
The functional hereditary units of BACTERIA.
An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
An enzyme that catalyzes the conversion of 4,5-dihydro-4-oxo-5-imidazolepropanoate to urocanate and water. EC 4.2.1.49.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
A class of enzymes that catalyze the cleavage of C-C, C-O, and C-N, and other bonds by other means than by hydrolysis or oxidation. (Enzyme Nomenclature, 1992) EC 4.
A species of gram-negative, aerobic bacteria isolated from soil and water as well as clinical specimens. Occasionally it is an opportunistic pathogen.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
Cell surface proteins that bind cyclic AMP with high affinity and trigger intracellular changes which influence the behavior of cells. The best characterized cyclic AMP receptors are those of the slime mold Dictyostelium discoideum. The transcription regulator CYCLIC AMP RECEPTOR PROTEIN of prokaryotes is not included nor are the eukaryotic cytoplasmic cyclic AMP receptor proteins which are the regulatory subunits of CYCLIC AMP-DEPENDENT PROTEIN KINASES.
Enzymes that catalyze the formation of a carbon-carbon double bond by the elimination of AMMONIA. EC 4.3.1.
Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.
A water-soluble, colorless crystal with an acid taste that is used as a chemical intermediate, in medicine, the manufacture of lacquers, and to make perfume esters. It is also used in foods as a sequestrant, buffer, and a neutralizing agent. (Hawley's Condensed Chemical Dictionary, 12th ed, p1099; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1851)
A family of transcription factors that contain two ZINC FINGER MOTIFS and bind to the DNA sequence (A/T)GATA(A/G).
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
A subdiscipline of genetics which deals with the genetic mechanisms and processes of microorganisms.
A dextrodisaccharide from malt and starch. It is used as a sweetening agent and fermentable intermediate in brewing. (Grant & Hackh's Chemical Dictionary, 5th ed)
Enzymes that catalyze the epimerization of chiral centers within carbohydrates or their derivatives. EC 5.1.3.
The functional hereditary units of FUNGI.
A disaccharide consisting of two glucose units in beta (1-4) glycosidic linkage. Obtained from the partial hydrolysis of cellulose.
A glycoside hydrolase found primarily in PLANTS and YEASTS. It has specificity for beta-D-fructofuranosides such as SUCROSE.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Proteins found in any species of fungus.
An enzyme that catalyzes the conversion of ATP and a D-hexose to ADP and a D-hexose 6-phosphate. D-Glucose, D-mannose, D-fructose, sorbitol, and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase. (From Enzyme Nomenclature, 1992) EC 2.7.1.1.
Enzymes that catalyze the endohydrolysis of 1,4-alpha-glycosidic linkages in STARCH; GLYCOGEN; and related POLYSACCHARIDES and OLIGOSACCHARIDES containing 3 or more 1,4-alpha-linked D-glucose units.
A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A species of imperfect fungi from which the antibiotic nidulin is obtained. Its teleomorph is Emericella nidulans.
An enzyme that catalyzes the conversion of acetoin to diacetyl in the presence of NAD.
A monosaccharide in sweet fruits and honey that is soluble in water, alcohol, or ether. It is used as a preservative and an intravenous infusion in parenteral feeding.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Proteins obtained from ESCHERICHIA COLI.
A group of enzymes that catalyzes the conversion of ATP and D-glucose to ADP and D-glucose 6-phosphate. They are found in invertebrates and microorganisms, and are highly specific for glucose. (Enzyme Nomenclature, 1992) EC 2.7.1.2.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
In eukaryotes, a genetic unit consisting of a noncontiguous group of genes under the control of a single regulator gene. In bacteria, regulons are global regulatory systems involved in the interplay of pleiotropic regulatory domains and consist of several OPERONS.
A rod-shaped bacterium isolated from milk and cheese, dairy products and dairy environments, sour dough, cow dung, silage, and human mouth, human intestinal contents and stools, and the human vagina.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
An enzyme that catalyzes the formation of CoA derivatives from ATP, acetate, and CoA to form AMP, pyrophosphate, and acetyl CoA. It acts also on propionates and acrylates. EC 6.2.1.1.
Enzymes that catalyze the interconversion of aldose and ketose compounds.
An enzyme that catalyzes reversibly the formation of galactose 1-phosphate and ADP from ATP and D-galactose. Galactosamine can also act as the acceptor. A deficiency of this enzyme results in GALACTOSEMIA. EC 2.7.1.6.
The regulatory elements of an OPERON to which activators or repressors bind thereby effecting the transcription of GENES in the operon.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses.
An enzyme that catalyzes the oxidation of protocatechuate to 3-carboxy-cis-cis-muconate in the presence of molecular oxygen. It contains ferric ion. EC 1.13.11.3.
Stable carbon atoms that have the same atomic number as the element carbon, but differ in atomic weight. C-13 is a stable carbon isotope.
A pentose active in biological systems usually in its D-form.
A LEUCINE and DNA-binding protein that is found primarily in BACTERIA and ARCHAEA. It regulates GENETIC TRANSCRIPTION involved in METABOLISM of AMINO ACIDS in response to the increased concentration of LEUCINE.
The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Enzymes that catalyze the exohydrolysis of 1,4-alpha-glucosidic linkages with release of alpha-glucose. Deficiency of alpha-1,4-glucosidase may cause GLYCOGEN STORAGE DISEASE TYPE II.
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
Enzymes that hydrolyze O-glucosyl-compounds. (Enzyme Nomenclature, 1992) EC 3.2.1.-.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Mutation process that restores the wild-type PHENOTYPE in an organism possessing a mutationally altered GENOTYPE. The second "suppressor" mutation may be on a different gene, on the same gene but located at a distance from the site of the primary mutation, or in extrachromosomal genes (EXTRACHROMOSOMAL INHERITANCE).
An enzyme that catalyzes the reversible hydration of cis-aconitate to yield citrate or isocitrate. It is one of the citric acid cycle enzymes. EC 4.2.1.3.
A group of enzymes that catalyzes the transfer of a phosphate group onto a nitrogenous group acceptor. EC 2.7.3.
A group of enzymes that catalyze the hydrolysis of alpha- or beta-xylosidic linkages. EC 3.2.1.8 catalyzes the endo-hydrolysis of 1,4-beta-D-xylosidic linkages; EC 3.2.1.32 catalyzes the endo-hydrolysis of 1,3-beta-D-xylosidic linkages; EC 3.2.1.37 catalyzes the exo-hydrolysis of 1,4-beta-D-linkages from the non-reducing termini of xylans; and EC 3.2.1.72 catalyzes the exo-hydrolysis of 1,3-beta-D-linkages from the non-reducing termini of xylans. Other xylosidases have been identified that catalyze the hydrolysis of alpha-xylosidic bonds.
An enzyme that catalyzes the formation of glycerol 3-phosphate from ATP and glycerol. Dihydroxyacetone and L-glyceraldehyde can also act as acceptors; UTP and, in the case of the yeast enzyme, ITP and GTP can act as donors. It provides a way for glycerol derived from fats or glycerides to enter the glycolytic pathway. EC 2.7.1.30.
Nucleic acid sequences involved in regulating the expression of genes.
A product of fermentation. It is a component of the butanediol cycle in microorganisms. In mammals it is oxidized to carbon dioxide.
A colorless, toxic liquid with a strong aromatic odor. It is used to make rubbers, polymers and copolymers, and polystyrene plastics.
Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.
Life or metabolic reactions occurring in an environment containing oxygen.
Alcohols derived from the aryl radical (C6H5CH2-) and defined by C6H5CHOH. The concept includes derivatives with any substituents on the benzene ring.
An enzyme in the tryptophan biosynthetic pathway. EC 4.1.1.48.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
An essential amino acid that is required for the production of HISTAMINE.
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
Any method used for determining the location of and relative distances between genes on a chromosome.
A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.
The rate dynamics in chemical or physical systems.
A species of bacteria whose spores vary from round to elongate. It is a common soil saprophyte.
A widely used industrial solvent.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
Bacterial repressor proteins that bind to the LAC OPERON and thereby prevent the synthesis of proteins involved in catabolism of LACTOSE. When lactose levels are high lac repressors undergo an allosteric change that causes their release from the DNA and the resumption of lac operon transcription.
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
An enzyme that catalyzes the conversion of D-fructose 1,6-bisphosphate and water to D-fructose 6-phosphate and orthophosphate. EC 3.1.3.11.
Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.
Reversibly catalyzes the oxidation of a hydroxyl group of sugar alcohols to form a keto sugar, aldehyde or lactone. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.; EC 1.1.2. and EC 1.1.99.
Deoxyribonucleic acid that makes up the genetic material of fungi.
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Galactosides in which the oxygen atom linking the sugar and aglycone is replaced by a sulfur atom.
Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.
A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99).
Enzymes which catalyze the endohydrolysis of 1,4-beta-D-xylosidic linkages in XYLANS.
A method for determining the sequence specificity of DNA-binding proteins. DNA footprinting utilizes a DNA damaging agent (either a chemical reagent or a nuclease) which cleaves DNA at every base pair. DNA cleavage is inhibited where the ligand binds to DNA. (from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
Enzymes that catalyze the breakage of a carbon-oxygen bond leading to unsaturated products via the removal of water. EC 4.2.1.
A non-essential amino acid that is synthesized from GLUTAMIC ACID. It is an essential component of COLLAGEN and is important for proper functioning of joints and tendons.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
High molecular weight polysaccharides present in the cell walls of all plants. Pectins cement cell walls together. They are used as emulsifiers and stabilizers in the food industry. They have been tried for a variety of therapeutic uses including as antidiarrheals, where they are now generally considered ineffective, and in the treatment of hypercholesterolemia.
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
Decarboxylated arginine, isolated from several plant and animal sources, e.g., pollen, ergot, herring sperm, octopus muscle.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
A series of oxidative reactions in the breakdown of acetyl units derived from GLUCOSE; FATTY ACIDS; or AMINO ACIDS by means of tricarboxylic acid intermediates. The end products are CARBON DIOXIDE, water, and energy in the form of phosphate bonds.
An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.
Motifs in DNA- and RNA-binding proteins whose amino acids are folded into a single structural unit around a zinc atom. In the classic zinc finger, one zinc atom is bound to two cysteines and two histidines. In between the cysteines and histidines are 12 residues which form a DNA binding fingertip. By variations in the composition of the sequences in the fingertip and the number and spacing of tandem repeats of the motif, zinc fingers can form a large number of different sequence specific binding sites.
An enzyme that catalyzes the conversion of ATP, L-glutamate, and NH3 to ADP, orthophosphate, and L-glutamine. It also acts more slowly on 4-methylene-L-glutamate. (From Enzyme Nomenclature, 1992) EC 6.3.1.2.
An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans.
Deletion of sequences of nucleic acids from the genetic material of an individual.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.
A family of compounds containing an oxo group with the general structure of 1,5-pentanedioic acid. (From Lehninger, Principles of Biochemistry, 1982, p442)
A class of enzymes that catalyze geometric or structural changes within a molecule to form a single product. The reactions do not involve a net change in the concentrations of compounds other than the substrate and the product.(from Dorland, 28th ed) EC 5.
The generic name for the group of aliphatic hydrocarbons Cn-H2n+2. They are denoted by the suffix -ane. (Grant & Hackh's Chemical Dictionary, 5th ed)
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Derivatives of GLUTAMIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the 2-aminopentanedioic acid structure.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A species of gram-positive, facultatively anaerobic bacteria in the family STREPTOCOCCACEAE. It is a normal inhabitant of the human oral cavity, and causes DENTAL PLAQUE and ENDOCARDITIS. It is being investigated as a vehicle for vaccine delivery.
A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that causes vascular wilts on a wide range of plant species. It was formerly named Erwinia chrysanthemi.
A polyhydric alcohol with about half the sweetness of sucrose. Sorbitol occurs naturally and is also produced synthetically from glucose. It was formerly used as a diuretic and may still be used as a laxative and in irrigating solutions for some surgical procedures. It is also used in many manufacturing processes, as a pharmaceutical aid, and in several research applications.
Transport proteins that carry specific substances in the blood or across cell membranes.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals.
Product of the oxidation of ethanol and of the destructive distillation of wood. It is used locally, occasionally internally, as a counterirritant and also as a reagent. (Stedman, 26th ed)
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Any member of the class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting molecules, e.g., ESTERASES, glycosidases (GLYCOSIDE HYDROLASES), lipases, NUCLEOTIDASES, peptidases (PEPTIDE HYDROLASES), and phosphatases (PHOSPHORIC MONOESTER HYDROLASES). EC 3.
A species of gram-negative, aerobic bacteria that causes formation of root nodules on some, but not all, types of sweet clover, MEDICAGO SATIVA, and fenugreek.
A hydrolase enzyme that converts L-asparagine and water to L-aspartate and NH3. EC 3.5.1.1.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)
A theoretical representative nucleotide or amino acid sequence in which each nucleotide or amino acid is the one which occurs most frequently at that site in the different sequences which occur in nature. The phrase also refers to an actual sequence which approximates the theoretical consensus. A known CONSERVED SEQUENCE set is represented by a consensus sequence. Commonly observed supersecondary protein structures (AMINO ACID MOTIFS) are often formed by conserved sequences.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
Heat and stain resistant, metabolically inactive bodies formed within the vegetative cells of bacteria of the genera Bacillus and Clostridium.
A large group of membrane transport proteins that shuttle MONOSACCHARIDES across CELL MEMBRANES.
Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.
A polysaccharide-producing species of STREPTOCOCCUS isolated from human dental plaque.
Interruption or suppression of the expression of a gene at transcriptional or translational levels.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
The turning off of GENETIC TRANSCRIPTION in certain regions of CHROMATIN without changes in the DNA sequence. Typically epigenetic repression is a way that developmental changes are programmed at the cellular level.
A diuretic and renal diagnostic aid related to sorbitol. It has little significant energy value as it is largely eliminated from the body before any metabolism can take place. It can be used to treat oliguria associated with kidney failure or other manifestations of inadequate renal function and has been used for determination of glomerular filtration rate. Mannitol is also commonly used as a research tool in cell biological studies, usually to control osmolarity.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
Organic compounds containing the carboxy group (-COOH). This group of compounds includes amino acids and fatty acids. Carboxylic acids can be saturated, unsaturated, or aromatic.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
A metabolic process that converts GLUCOSE into two molecules of PYRUVIC ACID through a series of enzymatic reactions. Energy generated by this process is conserved in two molecules of ATP. Glycolysis is the universal catabolic pathway for glucose, free glucose, or glucose derived from complex CARBOHYDRATES, such as GLYCOGEN and STARCH.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
An enzyme of the lyase class that catalyzes the formation of CYCLIC AMP and pyrophosphate from ATP. EC 4.6.1.1.
Vertical transmission of hereditary characters by DNA from cytoplasmic organelles such as MITOCHONDRIA; CHLOROPLASTS; and PLASTIDS, or from PLASMIDS or viral episomal DNA.
Bacterial proteins that are used by BACTERIOPHAGES to incorporate their DNA into the DNA of the "host" bacteria. They are DNA-binding proteins that function in genetic recombination as well as in transcriptional and translational regulation.
Enzymes from the transferase class that catalyze the transfer of acyl groups from donor to acceptor, forming either esters or amides. (From Enzyme Nomenclature 1992) EC 2.3.

Transcriptional analysis of differential carbohydrate utilization by Clostridium acetobutylicum. (1/31)

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Catabolite repression of Aox in Pichia pastoris is dependent on hexose transporter PpHxt1 and pexophagy. (2/31)

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Simultaneous consumption of pentose and hexose sugars: an optimal microbial phenotype for efficient fermentation of lignocellulosic biomass. (3/31)

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Escherichia coli mhpR gene expression is regulated by catabolite repression mediated by the cAMP-CRP complex. (4/31)

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Metabolomics of Mycobacterium tuberculosis reveals compartmentalized co-catabolism of carbon substrates. (5/31)

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Computational prediction of the Crc regulon identifies genus-wide and species-specific targets of catabolite repression control in Pseudomonas bacteria. (6/31)

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cAMP receptor protein (CRP) positively regulates the yihU-yshA operon in Salmonella enterica serovar Typhi. (7/31)

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Multifactorial induction of an orphan PKS-NRPS gene cluster in Aspergillus terreus. (8/31)

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I can only tell you that there has been an obvious change in the medical arena that has affected our mental health via our gut. In years gone by, an antibiotic prescription was always accompanied by instructions for simultaneous consumption of Acidophilus to replace the gut microbes that the antibiotics would destroy. This was in the time of penicillin, sulfur, and ampicillin.. Todays drugs, like Cipro, Biaxin and Cleocin, (Which would knock out a city block of microbes!), arent prescribed with the same recommendation. Why has this important step to keep our gut healthy been overlooked? Could this be the opening for the IBS, colitis and other inflammatory diseases affecting millions of people nationwide?. Fortunately, thanks to the Cork researchers, we have a simple and natural solution for the problem! Those of us who take antibiotics, birth control pills or other steroids, which upset the gut food balance, can help ourselves. We can add back these essential probiotics to our gut so our ...
This study aimed to explore a novel strategy for the simultaneous consumption of soluble soybean polysaccharides (SSPS) and insoluble genistein to improve the bioavailability of genistein and its prevention against obesity and metabolic syndrome in high-fat diet (HFD)-induced obese mice. C57BL/6J mice were fed a normal diet and HFD supplemented or not (n = 8) with SSPS (2.5%), genistein (0.5%) and ...
This study aimed to explore a novel strategy for the simultaneous consumption of soluble soybean polysaccharides (SSPS) and insoluble genistein to improve the bioavailability of genistein and its prevention against obesity and metabolic syndrome in high-fat diet (HFD)-induced obese mice. C57BL/6J mice were f
Crc (catabolite repression control) protein of Pseudomonas aeruginosa has shown to be involved in carbon regulation of several pathways. In this study the role of Crc in catabolite repression control has been studied in Pseudomonas putida. The bkd op ...
URC will again have a significant presence at this years Union World Conference on Lung Health from October 30-November 3 in Paris, France. In many of the 22 countries that account for more than 80% of TB cases worldwide, we are supporting national TB programs through innovative approaches to address systemic barriers to effective prevention and treatment. The conference provides an opportunity to share our TB work with thousands of health care professionals, researchers, and advocates.
The USAID Applying Science to Strengthen and Improve Systems (ASSIST) Project published a 24-page technical report, Tanzania-Kenya knowledge exchange for the Partnership for HIV-Free Survival, in May 2016 for the Partnership for HIV-Free Survival (PHFS) Project. The knowledge exchange was held between PHFS-participating country representatives from Tanzania and Kenya in partnership with the Ministries of Health of Tanzania and Kenya. It was organized by the ASSIST Project with support from University Research Co., LLC (URC), the Institute for Healthcare Improvement (IHI), and the Johns Hopkins Center for Communication Programs (CCP). The report was authored by Patty Webster of IHI, Sidhartha Deka of CCP, and Amy Stern of URC.
This website uses cookies to improve your experience while you navigate through the website. Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. We also use third-party cookies that help us analyze and understand how you use this website. These cookies will be stored in your browser only with your consent. You also have the option to opt-out of these cookies. But opting out of some of these cookies may affect your browsing experience ...
billed the concert as a jazzberry event with strawberries and cream, tea and coffee, and other refreshments included in the modest admission charge. Octogenarian MacDonald did the talking, his Canadian accent adding an extra dollars worth to the story telling. Softly spoken, to this day Benny Goodman his man, the clarinetist sat between James Birkett, guitar, and Bruce Rollo, double bass. Having heard the VJT on numerous occasions, here in Morpeth it occurred to your reviewer that Mr MacDonald doesnt count in any of the tunes. The tempo determined in his mind, he began to play. Messrs Birkett and Rollo were on it instantly. First Summertime then, as MacDonald observed wryly, time for Autumn Leaves. Our Canadian ex-pat enjoys putting Birkett and Rollo to the test with an up tempo number. Straight in, a breakneck After Youve Gone. How do they do it? Marvellous! An exquisite A Nightingale Sang in Berkeley Square held the audience spellbound, a swift It Dont Mean a Thing breaking the spell. ...
Vinyasa Power Joga je systém yogového cvičenia, ktorý je zameraný na plynulé prepájanie pohybu s dychom. Počas hodiny sú podrobne vysvetľované princípy každej yogovej pozície (asány) a je vždy možné zvoliť aj ich jednoduchšie varianty. Aj preto je hodina vhodná tak pre začiatočníkov, ako aj pokročilých cvičencov, obtiažnosť je možné zvoliť si individuálne. Celkovo je však Vinyasa Power Joga dynamickejšia v nadväznosti jednotlivých asán. Určite si zacvičia všetci, ktorí majú vysoké nároky na náročnosť a aj na samotnú techniku.. ...
The use of inedible lignocellulosic biomasses for biomanufacturing provides important environmental and economic benefits for society. Efficient co-utilization of lignocellulosic biomass-derived sugars, primarily glucose and xylose, is critical for the viability of lignocellulosic biorefineries. However, the phenomenon of glucose repression prevents co-utilization of both glucose and xylose in cellulosic hydrolysates. To circumvent glucose repression, co-utilization of cellobiose and xylose by Bacillus coagulans NL01 was investigated. During co-fermentation of cellobiose and xylose, B. coagulans NL01 simultaneously consumed the sugar mixtures and exhibited an improved lactic acid yield compared with co-fermentation of glucose and xylose. Moreover, the cellobiose metabolism of B. coagulans NL01 was investigated for the first time. Based on comparative genomic analysis, two gene clusters that encode two different operons of the cellobiose-specific phosphoenolpyruvate-dependent phosphotransferase system
TY - JOUR. T1 - Carbon catabolite repression of type IV pilus-dependent gliding motility in the anaerobic pathogen Clostridium perfringens. AU - Mendez, Marcelo. AU - Huang, I. Hsiu. AU - Ohtani, Kaori. AU - Grau, Roberto. AU - Shimizu, Tohru. AU - Sarker, Mahfuzur R.. PY - 2008/1/1. Y1 - 2008/1/1. N2 - Clostridium perfringens is an anaerobic, gram-positive, spore-forming bacterium responsible for the production of severe histotoxic and gastrointestinal diseases in humans and animals. In silico analysis of the three available genome-sequenced C. perfringens strains (13, SM101, and ATCC13124) revealed that genes that encode flagellar proteins and genes involved in Chemotaxis are absent. However, those strains exhibit type IV pilus (TFP)-dependent gliding motility. Since carbon catabolite regulation has been implicated in the control of different bacterial behaviors, we investigated the effects of glucose and other readily metabolized carbohydrates on C. perfringens gliding motility. Our results ...
The home-automation and networking companies that cater to home technology integrators seem to flit in and out of the security channel, as they wonder how to attract dealers that live on recurring monthly revenue (RMR).. This year, there were no biggies that really stood out, but URC and Pro Control (from RTI) were there with tabletops, promoting new home-control products sold through security-oriented distributors.. URC. In 2016, URC introduced HomeSet, a basic A/V and home-automation controller that starts at just $299 for a hub. The company demonstrated the product this year at ISC.. The fact that it doesnt provide RMR per se doesnt faze URC. Its actually an advantage for an industry that typically waits two or three years to break even on a security system.. It provides profit right now, says URCs Andre Lalande. You dont have to wait for the ROI later on.. HomeSet does not integrate directly with security systems; however, it accommodates third-party apps through a drop-down menu, ...
Dear Friends,. our Daily Devotion writers have been asked to write prayers to help us through these strange times. Each Thursday a new prayer will be published here. https://urc.org.uk/prayers-during-the-pandemic. which we hope will give voice to the many different emotions we are all feeling.. Do check back each Thursday - I wrote this weeks prayer, more will appear in the weeks to come.. best wishes. Andy ...
AMSTERDAM, NL (February 11, 2020)-URC, a global leader in smart automation and control for residential and commercial applications, launched today a 2-way IP module for integration of its award-winning Total Control system with Symetrix Radius NX, Prism, Solus and Edge DSP models. The module, available today to authorized URC installers via URCs dealer portal, allows commercial facilities managers using any URC Total Control touch screen or the Total Control smartphone application to control independent channels or zones of audio routed thru compatible Symetrix DSP models.. The addition of these new models from Symetrix to our portfolio of two-way modules greatly expands the commercial applications - from bars and restaurants, houses of worship, meeting rooms, to larger venues such as whole resort facilities, banquet halls and stadiums, said Lars Granoe, URCs Vice President of Product Management. Symetrix is world-renown for delivering high performance audio DSP hardware and software, ...
SUMMARY:. Through training, mentoring and the introduction of quality assurance (QA) tools and approaches, University. Research Co., LLC/Quality Assurance Project (Health Care Improvement - URC/QAP/HCI) will assist South. African Department of Health (DOH) facilities in five provinces to improve the quality of PMTCT and follow-. up services. Facilities identified for support differ from those of other PEPFAR partners. Training and other. activities are coordinated to avoid duplication and redundancy. URC/QAP will capacitate healthcare workers. to ensure rapid identification and referral of HIV-infected pregnant women and their babies to appropriate. services. The essential elements of QA include technical compliance with evidence-based norms and. standards, interpersonal communication and counseling and increasing organizational efficiency. The major. emphasis areas for this activity are QA and supportive supervision, with minor emphasis on development of. networks, linkages, referral systems, ...
The United Reformed Church has launched a new resource to help people explore how they can respond to the current needs, opportunities and injustices in their local neighbourhoods.
Biotin-11-dUTP is able to replace dTTP in growing DNA-strands and is used for efficient non-radioactive DNA-labelling. As a general rule, the labelled DNA is then detected using streptavidin-conjugates by colorimetric (e.g. streptavidin-AP) or fluorometric (e.g. fluorescein-streptavidin) assays ...
The [email protected] Centre provides a platform for research students to deposit their Ph.D. theses and make it available to the entire scholarly community in open access ...
University Records Center Ltd. (URC) was set up in 2010 to enable employers to assess the academic results of Japanese universities.
Catabolite repression was extensively studied in Escherichia coli. E. coli grows faster on glucose than on any other carbon source. For example, if E. coli is placed on an agar plate containing only glucose and lactose, the bacteria will use glucose first and lactose second. When glucose is available in the environment, the synthesis of β-galactosidase is under repression due to the effect of catabolite repression caused by glucose. The catabolite repression in this case is achieved through the utilization of phosphotransferase system. An important enzyme from the phosphotransferase system called Enzyme II A (EIIA) plays a central role in this mechanism. There are different catabolite-specific EIIA in a single cell, even though different bacterial groups have specificities to different sets of catabolites. In enteric bacteria one of the EIIA enzymes in their set is specific for glucose transport only. When glucose levels are high inside the bacteria, EIIA mostly exists in its unphosphorylated ...
(Blogpost from Usha Periyanayagam in Cambodia) This project Im working on is through Stanfords collaboration with URC, University Research Consortion, and is funded by USAID. URC is in the middle of a five year project (Better Health Services) with the Cambodia government that has wide goals, including providing funding for very low income patients, implementing…
Carbon catabolite repression (CCR) is a mechanism by which micro-organisms preferentially utilize more easily metabolizable carbon sources in comparison to less easily metabolizable carbon sources. It prevents the organisms from unnecessary expenditure of energy and enables them to exploit the nutrients in appropriate manner. It represents a complex system of gene regulation. The main aim of this study was to study the intracellular localization of proteins involved in CCR including CreA, CreB, CreC and CreD in A. nidulans in repressing and derepressing conditions. The major regulatory protein involved in CCR in A. nidulans is CreA. It is a DNA-binding repressor, but very little is known about the molecular events that allow CreA function to result in appropriate regulation in response to carbon source. To determine the amount and localization of CreA in different carbon sources, strains were made over-expressing GFP and HA tagged CreA. Western analysis showed that high levels of full length ...
pkgsrc 2009Q1 bulk build results NetBSD 4.0/powerpc Compiler: gcc Summary: Build started: Mon May 11 15:59:54 2009 UTC Build ended: Fri May 15 10:51:33 2009 UTC Successfully packaged: 6268 Packages really broken: 261 Pkgs broken due to them: 1433 Total broken: 1694 Not packaged: 0 Not available: 323 Total: 1694 Packages not listed here resulted in a binary package. The build report, including logs of failed/not-packaged is available from: ftp://malus.urc.uninett.no/pub/NetBSD/pkgstat/20090511.1545/report.html Top Ten Offenders Package Breaks Maintainer -------------------------------------------------------------- devel/libffi 1293 asau%[email protected] fonts/libfontenc 49 joerg%[email protected] x11/xkbcomp 44 joerg%[email protected] math/p5-Math-Pari 8 pkgsrc-users%[email protected] www/p5-Catalyst-Devel 7 pkgsrc-users%[email protected] emulators/darwin_lib 6 abacau%[email protected] math/cln 6 adam%[email protected] x11/libXprintUtil 6 joerg%[email protected] ...
In October last year raised £426.50 and donations of £70 and £143 raised at the URC Thanksgiving supper made a grand total sent to the fund of £639.50. Tuesday 7.30am - 30 minute Prayer Sanctuary, 5pm - Pebbles (ages 5-9) Thursday, February 2, at 2pm -
chains in the Genus database with same CATH superfamily 1YWU A; 2GJG A; 2L74 A; 4HG6 A; 4F48 B; 5KEC A; 4XRN A; 4P02 A; 1YLN A; 3DSG A; 5KGO A; 2RDE A; 4P00 A; 5EJZ A; 3KYF A; 4RT0 A; 4I86 A; 4RT1 A; 5EIY A; 3CNR A; 5EJ1 A; 4FOU C; 3KYG A; 5KED A; 5EJL A; #chains in the Genus database with same CATH topology 1EAW A; 3M3S A; 4H4F A; 1TLD A; 1HAI H; 1A3B H; 2FOA A; 5TJX A; 1UVS H; 1QRZ A; 2FOM B; 1GJ6 A; 1NM6 A; 1AKS B; 1HAV A; 1O2G H; 1Q2W A; 1F0S A; 2FO9 A; 2KCA A; 1DUA A; 2FEQ H; 3PMA B; 1G37 A; 1H8H D; 4AB9 A; 5C5N A; 3U8R H; 2QF0 A; 3VFE A; 1FY1 A; 3SV7 A; 3URC A; 2VVU A; 4IW4 E; 1WU1 A; 1O2Y A; 5LPF A; 4K1S A; 3SNE A; 3Q76 A; 2F9B H; 2G81 E; 1AFQ C; 3RXA A; 1C1N A; 1AB9 B; 3TH4 H; 1ESB A; 5FL7 D; 1C5M D; 1AE5 A; 2QYI A; 3HK3 B; 1RXP A; 4OGY A; 4ABI A; 4XBD A; 3SUG A; 1QR3 E; 1FMG A; 2Y7Z A; 1PPE E; 1UTP A; 2BLV A; 4UFE H; 4F49 A; 5L2Z H; 1ZHR A; 1LO6 A; 2PSY A; 3T26 A; 1OWE A; 1TNJ A; 2V3H H; 1DXW A; 3ZVA A; 2ZF0 H; 1SLU B; 5JB8 S; 1OYT H; 1O2H A; 4Y6D A; 1Z6E A; 3KQE A; 2JJ1 D; 4AN7 A; 2VIV ...
This website uses cookies to improve your experience while you navigate through the website. Out of these cookies, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. We also use third-party cookies that help us analyze and understand how you use this website. These cookies will be stored in your browser only with your consent. You also have the option to opt-out of these cookies. But opting out of some of these cookies may have an effect on your browsing experience ...
We weighted 10 top-rated co2crea case products over the past 3 years. Uncover which co2crea case is best for you. Filter by model, type, custom bundle and waterproof.
Understanding the genetic pathways that regulate how pathogenic fungi respond to their environment is paramount to developing effective mitigation strategies against disease. Carbon catabolite repression (CCR) is a global regulatory mechanism found in a wide range of microbial organisms that ensures the preferential utilization of glucose over less favourable carbon sources, but little is known about the components of CCR in filamentous fungi. Here we report three new mediators of CCR in the devastating rice blast fungus Magnaporthe oryzae: the sugar sensor Tps1, the Nmr1-3 inhibitor proteins, and the multidrug and toxin extrusion (MATE)-family pump, Mdt1. Using simple plate tests coupled with transcriptional analysis, we show that Tps1, in response to glucose-6-phosphate sensing, triggers CCR via the inactivation of Nmr1-3. In addition, by dissecting the CCR pathway using Agrobacterium tumefaciens-mediated mutagenesis, we also show that Mdt1 is an additional and previously unknown regulator of ...
The catabolism of glucose and xylose was studied in a wild type and creA deleted (carbon catabolite de-repressed) strain of Aspergillus nidulans. Both strains were cultivated in bioreactors with either glucose or xylose as the sole carbon source, or in the presence of both sugars. In the cultivations on single carbon sources, it was demonstrated that xylose acted as a carbon catabolite repressor (xylose cultivations), while the enzymes in the xylose utilisation pathway were also subject to repression in the presence of glucose (glucose cultivations). In the wild type strain growing on the sugar mixture, glucose repression of xylose utilisation was observed; with xylose utilisation occurring only after glucose was depleted. This phenomenon was not seen in the creA deleted strain, where glucose and xylose were catabolised simultaneously. Measurement of key metabolites and the activities of key enzymes in the xylose utilisation pathway revealed that xylose metabolism was occurring in the creA ...
The ACO1 gene, encoding mitochondrial aconitase of Saccharomyces cerevisiae, is required both for oxidative metabolism and for glutamate prototrophy. This gene is subject to catabolite repression; the ACO1 mRNA level is further reduced when glutamate is supplied with glucose. To further explore regulation of ACO1 expression, we have screened for mutations that reduce expression of an ACO1-lacZ fusion borne on a multicopy vector. We identified a gene required for wild-type expression of ACO1 only under catabolite repression conditions. Sequencing of the corresponding cloned gene revealed that it is identical to RTG2 previously cloned as a pivotal gene in controlling interorganelle retrograde communication. Cells containing either the original rtg2-2 mutation or a null rtg2 allele are not petite but show a residual growth on minimum glucose medium with ammonium sulfate as the sole nitrogen source. This growth defect is partially restored by supplying aspartate or threonine, and fully with ...
Exploring the Christian religion and its teachings about Christ and his person and work as expressed in the United Reformed Churches of North America (URCNA) with Reformed sermons, discussion programs, and curriculum about Reformed theology through text and audio files.
M-am uitat in clipa aceea in jos, si nu vedeam josul, pamantul, solul, sau capatul inferior al scarii, insa am vazut, atunci, prima data, si m-a luat ameteala, ca scara este dreapta, abrupta, la 90 de grade, si imi era teribil de frica ca voi cadea si chiar nu stiam cum sa nu cad, pentru ca mai stiam ca va trebui sa ma ridic de pe treapta aceea, la un moment dat, si sa urc, indarat, deci mai sus, sa ma intorc cu spatele la treapta pe care sedeam acum si sa urc scara, in sus ( fac un pleonasm, dar sa fie foarte, foarte precis ce spun ). ...
From Tavistock Square (buses 59, 68, 91,168). From south-eastern corner of the Square (Eastern, if you are using a compass), where there are traffic lights, come along the full length of Tavistock Place. Church House is next to Lumen and clearly labelled high up as United Reformed Church House. From Grays Inn Road (buses 17, 45, 46) Alight at Eastman Dental Hospital (southwards) or Acton Street/Sidmouth Street (northwards) and walk West along Sidmouth Street beside Westminster Kingsway College, through Regent Square (pavement beside old regency terrace) to Church House.. From Russell Square underground (Piccadilly Line) Buses 7 and 188 terminate nearby.. Turn right out of the station, but cross two roads by zebra crossings at right angles, then walk below Brunswick Centre (concrete retail and housing complex originally built in 1970s). Turn left at end of Brunswick Centre, cross zebra below Renoir Cinema, then turn left, walk North beside garden of Brunswick Square and one more block before ...
p,Hydrogen peroxide (HO) and nitric oxide (NO·) are toxic metabolites that immune cells use to attack pathogens. These antimicrobials can be present at the same time in phagosomes, and it remains unclear how bacteria deal with these insults when simultaneously present. Here, using , we observed that simultaneous exposure to HO and NO· leads to prioritized detoxification, where enzymatic removal of NO· is impeded until HO has been eliminated. This phenomenon is reminiscent of carbon catabolite repression (CCR), where preferred carbon sources are catabolized prior to less desirable substrates; however, HO and NO· are toxic, growth-inhibitory compounds rather than growth-promoting nutrients. To understand how NO· detoxification is delayed by HO whereas HO detoxification proceeds unimpeded, we confirmed that the effect depended on Hmp, which is the main NO· detoxification enzyme, and used an approach that integrated computational modeling and experimentation to delineate and test potential ...
Dear netters, Can anyone here tell me some recently published reviews on gene cloning technique? Thanks. -- --------------------------------------K.U.Leuven--------------------- HUANG Zhong Lab of Gut Hormone =, http://urc1.cc.kuleuven.ac.be/~m9631766/ Gasthuisberg, B-3000 =, e-mail: huang.zhong at med.kuleuven.ac.be Leuven, Belgium =, Fax: 32-16-345939 Tel: 32-16-345750 ...
An easy-to-use directory for life science and biomedical research products. Find special deals on products, order catalogs and browse product lines from suppliers of reagents, antibodies, laboratory equipment, and more.
More information is available in the following publications:. Colombo, G. V., Urine Pretreatment Mixer (UPM), Final Report NASA Contract NAS8-36526, October 1986.. Colombo, G. V. and Putnam, D.F., Removal of Contaminant Gases from an Electrolytic Urine Pretreatment Process, SAE Technical Paper Series No. 77-ENAs-61, presented at 7th Intersociety Conference on Environmental Systems, San Francisco, CA, July 1977.. Colombo, G. V. and Putnam, D.F., Electrolytic Pretreatment Unit Gaseous Effluent Conditioning, Final Report NASA Contract NAS9-14217, August 1976.. ...
The acronym IBD identifies the ulcerative colitis (URC), Crohns disease (CD) and the undeterminate colitis (UC) 7. Inflammatory bowel diseases are characterized by variegated etiopathogenesis, probably autoimmune. They have in common a histological damage of a granulomatous/ulcerative kind and also the same manifestations which includes the alternation of remissions and exacerbations 1. They have a remarkable familiarity (13.5%) although it is more evident in CD than in URC. The incidence of IBD varies according to different geographical areas but with a steady increasing trend above all in CD and the diffusion seems to be linked to genetic factors (association with HLA-A2 and B 18) and to geographical factors ...
Background Many Firmicutes bacteria, including solvent-producing clostridia such as Clostridium acetobutylicum, are able to utilize xylose, an abundant carbon source in nature. Nevertheless, homology...
There is provided an engineered host cells comprising (a) one or more mutations in one or more endogenous genes encoding a protein associated with iron metabolism; and (b) at least one gene encoding a polypeptide having xylose isomerase activity, and methods of their use thereof.
Synthesis of penicillinamidohydrolase (penicillin acylase, EC 3.5.1.11) inEscherichia coli is subjected to the absolute catabolite repression by glucose and partial repression by acetate. Both types of catabolite repression of synthesis of the enzyme inEscherichia coli are substantially influenced by cyclic 3,′5′-adenosinemonophosphate (cAMP). Growth diauxie in a mixed medium containing glucose and phenylaoetic acid serving as carbon and energy sources is overcome by cAMP. cAMP does not influence the basal rate of the enzyme synthesis (without the inducer). Derepression of synthesis of penicillinamidohydrolasa by cAMP in a medium with glucose and inducer (phenylacetic acid) is associated with utilization of the inducer, due probably to derepression of other enzymes responsible for degradation of phenylacetic acid. Lactate can serve as a
MIU 1 p. . VOL. XXXIII. COLUMBUS, WEDNESDAY, FEBRUARY 14, 1872. tNO. 39. I xll I I II i ,f ir . i :t::to oCiuk a. m. HAYLER-KEMF tAHE. We publish In another column the majority report of the committee on Privileges and Election in the contented election cone in the Ohio Semite After ft very careful rcperuna. of thin report, we urc fully prepared to coincide in the opin Ion bo universally expmwed umour Repub Ikw. both In and oat.of the Senate, that it in art unanswerable argument. Hhorf, clear and comprehenHtve, the very utatc-mcnt of the proportion nddueed aro in tliemwlvena logical chain nf Impregnable reasoning. The author of the report-Mr. Hart, chairman of the committee Iiuh con lined himwlf strictly to the legal , and condtitutionul qnesUoiM involved in the cane, and I kip excluded all political or patriotic side-iiwuo which no naturally connect tlirmwlvc with the qucftionH, and muxt have ho htrongly tempted him in that direction. - w . The report hIiowh with great clenrneii that no ...
When both glucose and lactose are present in the medium, the transcription of the genes z, y and a are inhibited. This phenomenon is called catabolite repression or glucose effect. The effect of
Etacrynsäure - ein langjährig eingesetztes Diuretikum - und verschiedene ihrer Derivate sind als nichtpeptidische Inhibitoren von Cystein-Proteasen bekannt. Einige Amid-Derivate besitzen bezüglich des SARS-CoV sowohl enzymhemmende (SARS-CoV-Mpro) als auch antivirale Wirkung. Das elektrophile Strukturfragment des Moleküls, das als Michael-Akzeptor eine Reaktion mit dem active-site Cystein-Rest der Protease eingehen kann, ist die a,b-ungesättigte Carbonyleinheit in der Acyl-Seitenkette. Ausgehend von der Etacrynsäure als Leitstruktur wurden durch Strukturvariationen neue Etacrynsäure-Derivate, bevorzugt Amid-Derivate, dargestellt. Zusätzlich zu diesen Verbindungen wurden außerdem weitere Etacrynsäure-Derivate synthetisiert, die durch Biotin- und Deuterium-Markierung als Modellsubstanzen zum In-vitro-Nachweis des Wirkstoffes in biochemischen und spektroskopischen Assays dienen. Als deuterierte Spezies wurde mit der d9-Etacrynsäure ein hochdeuteriertes Wirkstoff-Analogon dargestellt. Der ...
An annual benchmark report commissioned by Michigans University Research Corridor again ranks the group of universities No. 1 in the nation for awarding advanced degrees in medicine and biological science.
Jesus family dont walk away from him when he doesnt get up and leave with them, they may have been concerned about the anger from the authorities that was starting to bubble up around him and the socio-political/spiritual movement that was forming, but they didnt walk away. And maybe that is one of the things about being a family, that you may worry about the people in it, but you stand with them, you support them, you encourage their passions and you enable them to move forward. My mother is one of those people, she doesnt always agree with me, there have been many discussions where I have challenged some of her opinions about social justice issues and there have been times when she has been exasperated with my liberal stance on many things, she has also questioned why I have faith and why I have answered a call to ministry, but those questions have not been asked cruelly, they have been asked from a point of concern and worry. Now by no means am I claiming that my mother is anything like ...
1MO1: Crystal Structure at 1.8 A of the Bacillus Subtil Catabolite Bacillus Subtilis Catabolite Repression Containing Protein (Crh) Reveals an Unexpected Swapping Domain as an Untertwinned Dimer
I am creating my syllabus for my U.S. history course since 1945. Because the course is at least as much thematic as chronological, I am crea ...
Exogenous anthrahydroquinone-2,6-disulfonate specifically increases xylose utilization during mixed sugar fermentation by Clostridium beijerinckii NCIMB ...
Under the severe glucose limitation at the low dilution rate used here, substantial fluxes through the PEP-glyoxylate cycle were expected (12, 25, 35). The precise regulation mechanism that effectively controlled this major flux rerouting from the TCA cycle to the PEP-glyoxylate cycle, however, was unclear. Based on in vivo flux and in vitro enzyme data on global regulator mutants, we demonstrate that PEP-glyoxylate cycle activity is strongly controlled by induction through the cAMP-CRP complex under the conditions applied. This finding is consistent with the reported increased mRNA and protein levels of PEP carboxykinase and glyoxylate shunt enzymes at a dilution rate of 0.1 h−1, relative to higher growth rates (25). Thus, growth rate-dependent PEP-glyoxylate cycle fluxes under glucose limitation (35) are apparently controlled by the intracellular cAMP level, which is elevated at dilution rates below 0.1 h−1 (32, 37). Strain-dependent differences in fluxes through the PEP-glyoxylate cycle ...
The discipline of soil science continued its wide coverage of research programme on organic matter management supported by (USAID), soil test crop response studies for fertilizer recommendation (URC), diagnosis of nutrient disorders of the root crops of the South Pacific Asian Centre for International Agricultural Research (ACIAR), management of sloping land (IBSRAM), and the studies on patterns of soil moisture depletion (URC). Most of these studies were carried out at the Alafua and Laloanea research fields of the Alafua Campus and some were on farmers fields.. The objective of the organic matter management study was to compare the effects of lime and green manure on dry matter yield and nutrient content of two consecutive 28-day cropping of maize seedlings, and on selected soil chemical properties of an acid Oxisol in Samoa. The growth responses of maize in this experiment demonstrated that soil acidity of an acid Oxisol can be alleviated by either lime or green manure additions. Furthermore, ...
Spiritual warfare (as we saw Sunday before last) can be hard to see, but there are visible things too. In last Sundays sermon, Jason took us through Matthew 12:33-37 and Jesus warning that what is true about us internally can be seen externally by our fruit, particularly our words.. THREE POINTS…. 1 Clear theology is always needed. We are all theologians, and while child-like faith is good, were not to go on subsisting on milk but on solid food. Jesuss illustration drives this home. He has just pointed to his own good fruit as testimony to who he really is, and now he points to the bad fruit of the pharisees to show who they really are. They can only do bad because their root is bad.. The Fourfold state of man is helpful in this: 1) In the garden, pre-Fall, we were able both to sin and to not sin; 2) As fallen people, we can only sin; 3) As regenerate people, we have changed hearts through unity with Christ and are now able not to sin (though while in these bodies we still do); and 4) We ...
Summary: A mutation in a gene designated gmdA has been found to lead to loss of ability of Aspergillus nidulans to use benzamide, phenylacetamide and several other amides as sole nitrogen sources for growth. The gmdAI lesion results in low levels of an enzyme, called the general amidase, which has activity for a wide range of amide substrates. This enzyme is repressed by certain nitrogen-containing metabolites, including ammonium, but is probably not regulated by induction or by carbon catabolite repression. Evidence is presented for the general amidase being distinct from the previously characterized acetamidase and formamidase enzymes. The data also indicate that there is a fourth amidase capable of the hydrolysis of valeramide and hexanamide.
Cameron McCoy is the AVP for Economic Engagement at Lehigh University. He is responsible for developing and executing an integrated strategic plan for the Lehigh Universitys economic engagement functions, including Industry Relations, Foundation Engagement, Career Services, Federal Agency - Industry - Lehigh relations, Research Park Development assessment, and some elements of Technology Transfer and Economic Development.. Prior to joining Lehigh in June of 2014, he served as the Executive Director of the Corporate Engagement Office (CEO) at the University of Oklahoma (OU) where he was responsible for the award winning University Research Campus (URC); supporting, coordinating, and integrating Research Enterprise activities with industry; simplifying external access to OU resources to maximize the impact of OU capabilities; expanding the engagement pipeline (current and new relationships) via targeted solutions; and growing external relationships with Oklahoma economic development ...
Margaret Hackett, 81, of Delray Beach said a nice man named Bob has been calling her on the telephone between 8 and 10 a.m. for the past three years.She had never met him, she said. She didn`t know
Carbon catabolite repression, or simply catabolite repression, is an important part of global control system of various ... Note that E. coli has a similar cAMP-independent catabolite repression mechanism that utilizes a protein called catabolite ... the synthesis of β-galactosidase is under repression due to the effect of catabolite repression caused by glucose. The ... is actually a misnomer since other carbon sources are known to induce catabolite repression.[citation needed] Catabolite ...
Stülke J, Hillen W. (1999). "Carbon catabolite repression in bacteria". Current Opinion in Microbiology. 2 (2): 195-201. doi: ... is required for the catabolite activator protein (CAP) to bind to DNA and activate the transcription of the lac operon, which ...
Two puzzles of catabolite repression relate to how cAMP levels are coupled to the presence of glucose, and secondly, why the ... Catabolite repression Griffiths, Anthony J.F.; Wessler, Susan R.; Carroll, Sean B.; Doebley, John (2015). An Introduction to ... Görke B, Stülke J (August 2008). "Carbon catabolite repression in bacteria: many ways to make the most out of nutrients". ... Binding of RNA polymerase to the promoter is aided by the cAMP-bound catabolite activator protein (CAP, also known as the cAMP ...
This phenomenon is known as catabolite repression. CAP plays an important role in catabolite repression, a well-known example ... p. 193 Catabolite+Activator+Protein at the US National Library of Medicine Medical Subject Headings (MeSH) v t e (Articles with ... Catabolite activator protein (CAP; also known as cAMP receptor protein, CRP) is a trans-acting transcriptional activator that ... Lawson CL, Swigon D, Murakami KS, Darst SA, Berman HM, Ebright RH (2004). "Catabolite activator protein: DNA binding and ...
4. Catabolite repression[1] When a microorganism is provided with a rapidly metabolizable carbon-energy source such as glucose ... A powerful method of overcoming the catabolite repression in the enzyme biosynthesis is a fed-batch culture in which glucose ... This phenomenon is known as catabolite repression. Many enzymes, especially those involved in catabolic pathways, are subject ... Substrate limitation also allows the metabolic control, to avoid osmotic effects, catabolite repression and overflow metabolism ...
Harchand, R. K.; Singh, S (1994). "Catabolite repression of cellulase biosynthesis in Streptomyces albaduncus". Journal of ...
Graham, I. A.; Denby, K. J.; Leaver, C. J. (1994). "Carbon Catabolite Repression Regulates Glyoxylate Cycle Gene Expression in ...
Vargas M; Noll KM (January 1996). "Catabolite repression in the hyperthermophilic bacterium Thermotoga neapolitana is ...
... is a small RNA found in Pseudomonas bacteria, which acts as a global regulator of carbon catabolite repression. In P. ... Sonnleitner E, Abdou L, Haas D (December 2009). "Small RNA as global regulator of carbon catabolite repression in Pseudomonas ... Sonnleitner E, Bläsi U (June 2014). "Regulation of Hfq by the RNA CrcZ in Pseudomonas aeruginosa carbon catabolite repression ... and catabolite repression is high. In the presence of poor sources of carbon, such as mannitol, CrcZ expression is high, ...
... with Catabolite repression (SOC) is SOB with added glucose. (Figures in parentheses are the masses required ...
Environmental stresses such as antibiotics and catabolite repression can influence toxin expression. The tcdA and tcdB genes ...
546 malT is regulated by catabolite repression via the catabolite activator protein. Genes under the control of malT include ...
The Catabolite repression control (Crc) protein participates in suppressing expression of several genes involved in utilization ... Zhang L, Chiang WC, Gao Q, Givskov M, Tolker-Nielsen T, Yang L, Zhang G (December 2012). "The catabolite repression control ... Sonnleitner E, Bläsi U (June 2014). "Regulation of Hfq by the RNA CrcZ in Pseudomonas aeruginosa carbon catabolite repression ... "Regulation of Hfq by the RNA CrcZ in Pseudomonas aeruginosa carbon catabolite repression". PLOS Genetics. 10 (6): e1004440. doi ...
Some of them, including UV5, has lost catabolite repression at the CAP site. Development into cloning vectors is known since ... Silverstone, AE; Arditti, RR; Magasanik, B (July 1970). "Catabolite-insensitive revertants of lac promoter mutants". ...
Later, she discovered another factor that boosts catabolite repression (catabolite modulator factor, or CMF). Ullmann ... In 1967 she showed that cAMP reverses catabolite repression in the bacterium E. coli. ...
Xylanase is a secondary metabolite controlled through gene-specific induction and carbon catabolite repression. Many ...
Peter GJ, Düring L, Ahmed A (March 2006). "Carbon catabolite repression regulates amino acid permeases in Saccharomyces ... "Sustained translational repression by eIF2α-P mediates prion neurodegeneration". Nature. 485 (7399): 507-11. Bibcode:2012Natur. ... "PML inhibits HIF-1alpha translation and neoangiogenesis through repression of mTOR". Nature. 442 (7104): 779-85. Bibcode: ...
"Transcription analysis of lignocellulolytic enzymes of Penicillium decumbens 114-2 and its catabolite-repression-resistant ...
Nakashima, Nobutaka; Tamura, Tomohiro (1 July 2012). "A new carbon catabolite repression mutation of Escherichia coli, mlc∗, ...
Global regulation of DNA molecules containing the gene for sucrose phosphorylase is performed by catabolite repression. First ...
Lee JH, Dobrogosz WJ (May 1983). "Effects of aerobic and anaerobic shock on catabolite repression in cyclic AMP suppressor ... In particular, his research focused on the phenomenon of catabolite repression, a regulatory system involving interactions of ... cyclic AMP, the catabolite repressor protein (CRP) complex, and the lac operon and other inducible systems in bacteria. The ...
Galactose is an alternate carbon source to the preferable glucose . The cAMP/CRP catabolite repression regulator is most likely ... 5. El Qaidi, S., Allemand, J.O., and Plumbridge, J. (2009). Repression of galP, the galactose transporter in Escherichia coli, ... so both of these proteins are required for repression (11). cAMP is what modulates CRP at the promoter. The cAMP-CRP complex ... a protein from the nagC gene which is responsible for N-acetylglucosamine repression (5). This study suspects that NagC ...
Carbon Catabolite Repression-Negative On TATA-less, or CCR4-Not, is a multiprotein complex that functions in gene expression. ...
2004). "HPr kinase/phosphorylase, a Walker motif A-containing bifunctional sensor enzyme controlling catabolite repression in ...
... which results in thn gene repression in catabolite repression conditions. EcpR1 sRNA RcsR1 small RNA Corbino KA, Barrick JE, ... catabolite repression conditions). This phenotype is due to the release of the negative effect of suhB on ThnR translation. Hfq ... a small non-coding RNA involved in catabolite repression of tetralin degradation genes in Sphingopyxis granuli strain TFA". ...
She found that the catabolite repression control protein, which regulates carbon metabolism, is essential for biofilm formation ...
"The Base-Pairing RNA Spot 42 Participates in a Multioutput Feedforward Loop to Help Enact Catabolite Repression in Escherichia ... The direct responsiveness of Spot 42 levels to glucose and cAMP is due to repression of spf expression by a cAMP-CRP (cAMP- ...
Vijay Kumar, N.; Rangarajan, P. N. (2011). "Catabolite repression of phosphoenolpyruvate carboxykinase by a zinc finger protein ... Rabies vaccine Foot-and-mouth disease Plasmodium falciparum Catabolite repression India portal Medicine portal Long link - ...
This explains why, when grown with glucose, E. coli will lose the ability to internalize AI-2 (because of catabolite repression ...
... biology approach reveals that overflow metabolism of acetate in Escherichia coli is triggered by carbon catabolite repression ...
... carbon catabolite repression) but independent of CreA when glucose is used as sole carbon source. Instead, it was shown to be ...
... super optimal broth with catabolite repression), colonies were patched onto selective plates using a toothpick and incubated at ...
... because the invertase is insensitive to catabolite repression by glucose, and because the activity of the glucose uptake system ...
... a variation of Cardiopulmonary Resuscitation Carbon catabolite repression, part of the adaptive metabolic control system ...
Catabolite repression, and the presence of β-glucan-hydrolyzing enzymes that attack botryosphaeran during the fermentation ...
... while it is repressed by nitrate and subject to CRP-mediated catabolite repression. DcuC family Zientz E, Six S, Unden G ( ...
Catabolite repression has also been observed in E. coli in the presence of other non-glucose sugars, such as arabinose and ... In E. coli, glucose catabolite repression is regulated by the phosphotransferase system, a multi-protein phosphorylation ... bacteria will often consume the sugars sequentially through a process known as catabolite repression. By repressing the ...
... catabolite repression control protein Criminal record check Cross Racing Championship Extreme 2005 (CRC 2005), a car-racing ...
... is a cell-surface protein and should not be confused with the unrelated carbon catabolite repression-negative on TATA-less ...
A proposed function of SLC46A3 of rising importance is the direct transport of maytansine-based catabolites from the lysosome ... Silencing mechanisms include mRNA cleavage and translation repression based on the level of complementarity between the miRNA ... Among the different types of antibody-drug conjugates (ADCs), maytansine-based noncleavable linker ADC catabolites, such as ... The clinical significance of SLC46A3 surrounds the protein's activity as a transporter of maytansine-based ADC catabolites. ...
... most commonly used lac or lac-derived promoters are based on the lacUV5 mutant which is insensitive to catabolite repression. ... "Catabolite-insensitive revertants of lac promoter mutants". Proceedings of the National Academy of Sciences USA. 66 (3): 773-9 ...
FAQ: Is T7 expression subject to catabolite repression in NEB T7 Express or SHuffle strains?. The T7 RNA polymerase gene is ... Home FAQs Is T7 expression subject to catabolite repression in NEB T7 Express or SHuffle strains? ... Since expression is controlled by the wt lac operon, glucose addition will result in catabolite repression. Thus, basal protein ...
Genes required for repression of cellulase gene expression when preferred carbon sources are present (carbon catabolite ... We show that disabling CRE1 repression and modulating the glucose response by deletion of col-26 restored growth of the Δvib-1 ... Cellulase production is subject to induction by the presence of plant biomass components and to repression by the availability ... but the mechanisms involved in crosstalk between repression versus induction of cellulase gene expression is poorly understood ...
Recovery from ultraviolet light-induced catabolite repression of L-arabinose isomerase.. Authors: Bhatnagar, D. Bhattacharya, A ... Recovery from ultraviolet light-induced catabolite repression of L-arabinose isomerase. Indian Journal of Biochemistry & ...
... and together with the catabolite repression control (Crc) protein, assembles into a translation-repression complex. Earlier ... Carbon catabolite repression in pectin digestion by the phytopathogen Dickeya dadantii. Journal of Biological Chemistry ...
Carbon catabolite repression in bacteria: many ways to make the most out of nutrients.. Gorke B, Stulke J., Nat. Rev. Microbiol ... The mechanisms of carbon catabolite repression in bacteria.. Deutscher J., Curr. Opin. Microbiol. 11(2), 2008 PMID: 18359269 ... Carbon catabolite repression by seryl phosphorylated HPr is essential to Streptococcus pneumoniae in carbohydrate-rich ... The alcohol dehydrogenase gene adhA in Corynebacterium glutamicum is subject to carbon catabolite repression.. Arndt A, ...
CATabolite repression. Saccharomyces cerevisiae. Necessary For Fitness 50% CAT5. 856347. CBP2. Cytochrome B mRNA Processing. ...
catabolite repression +. 0. cellular metabolic process +. 10349. collagen metabolic process +. 124. demethylation +. 69. ...
Catabolite repression in the D-serine deaminase system of Escherichia coli K-12. McFall E, Bloom FR. McFall E, et al. Among ... Isolation and characterization of catabolite-resistant mutants in the D-serine deaminase system of Escherichia coli K-12. Bloom ...
CcpA mediates the catabolite repression of tst in Staphylococcus aureus. Infect Immun. 2008;76:5093-9. DOIPubMedGoogle Scholar ... a glucose catabolite repressor CcpA (11), the staphylococcal accessory regulator A, σB (12) and the SaeRS 2-component system ( ...
MacGregor C.H., Wolff J. A., Arora S. K., Hylemon P. B., Phibbs P. V. 1992; Catabolite repression control in Pseudomonas ... evidence of carbon catabolite repression control. J Bacteriol 176:2354-2361 ... Mandelstam J., Jacoby G. A. 1965; Induction and multi-sensitive end-product repression in the enzymatic pathway de-grading ...
... expression sensitive to nitrogen catabolite repression ...
Catabolite Repression 39% Powered by Pure, Scopus & Elsevier Fingerprint Engine™ © 2022 Elsevier B.V. ...
Regulatory, loss of catabolite repression: growth on maltose + a-methylgucoside.. *References: *Reiner, A.M. 1977. Xylitol and ...
... a phenomenon known as carbon catabolite repression. Catabolite repression is mediated by Enzyme IIA Glucose (IIAGluc), which ... As mentioned earlier, our cells had to overcome carbon catabolite repression. Various mutations, including the insertion of two ...
Autoinduction media could be used, but this requires somewhat detailed knowledge about catabolite repression ...
"Identification of novel secreted fatty acids that regulate nitrogen catabolite repression in fission yeast". Sci. Rep. 6, 20856 ...
We show that the catabolite repression control (Crc) protein .... *Help. *About Us ...
A quantitative approach to catabolite repression in Escherichia coli.. Bettenbrock, Katja; Fischer, Sophia; Kremling, Andreas; ...
Kremling, A.; Kremling, S.; Bettenbrock, K.: Catabolite repression in Escherichia Coli - a comparison of modelling approaches. ... A quantitative approach to catabolite repression in Escherichia coli. Journal of Biological Chemistry 281, pp. 2578 - 2584 ( ...
Apparently there was catabolite repression which was more severe for culture medium g which contained the double of glucose ... Several authors have proposed that SSF system minimizes the catabolite repression (Ramesh and Lonsane, 1991; Aguilar, et al., ... It will be interesting to look for molecules that act as signal molecules in catabolite repression. Besides to study glucose ... del Castillo, T. and Ramos, J. L. (2007). Simultaneous catabolite repression between glucose and toluene metabolism in ...
Background Carbon catabolite repression (CCR) controls the order in which different carbon sources are metabolised. Although ... Abstract : Background Carbon catabolite repression (CCR) controls the order in which different carbon sources are metabolised. ...
Multiple Hfq-Crc target sites are required to impose catabolite repression on (methyl)phenol metabolism in Pseudomonas putida ... Multiple Hfq-Crc target sites are required to impose catabolite repression on (methyl)phenol metabolism in Pseudomonas putida ... The Crc and Hfq proteins of Pseudomonas putida cooperate in catabolite repression and formation of ribonucleic acid complexes ... AccR is a master regulator involved in carbon catabolite repression of the anaerobic catabolism of aromatic compounds in ...
Catabolite repression of α amylase gene expression in Bacillus subtilis involves a trans-acting gene product homologous to the ... CcpA is essential for catabolite repression; and Rex controls multiple genes affecting the redox status of the cells [27-30]. ... Ren C, Gu Y, Wu Y, Zhang W, Yang C, Yang S, Jiang W. Pleiotropic functions of catabolite control protein CcpA in butanol- ...
... to other carbon sources is known as succinate-mediated catabolite repression and it varies from the catabolite repression in ... The exact molecular mechanisms of the succinate-mediated catabolite repression is yet to be cleared up. One key note to make ...
A genome-wide analysis of carbon catabolite repression in Schizosaccharomyces pombe. Vassiliadis, Dane Aaron McKay (. 2018. ) ... at the transcriptional level to control the function of a conserved regulatory mechanism known as carbon catabolite repression ... and so there is limited knowledge of the range of genes that are subject to transcriptional repression, or of the functional ... is required for maximal transcriptional repression. Also implicated in this process is the transcriptional activator, Rst2, ...
Most microorganisms have evolved the ability to utilize it preferably due to carbon catabolite repression regulatory mechanism ... A brief insight on carbon catabolite repression and phosphostransferase system were made. It also highlighted the biogas ... BET bromodomain blockade using small-molecule inhibitors gives rise to selective repression of the transcriptional network ...
14Inui M, Vertès AA and Yukawa H. Reverse catabolite repression and the regulation of CO2 fixation Rhodobacter and related ... exerts different degrees of repression depending on the location of its binding sites within the three target promoter regions ... Inui M and Yukawa H Translation efficiency of antiterminator proteins is a determinant for the difference in glucose repression ...
Paulsen IT, Chauvaux S, Choi P, Saier MH: Characterization of glucose specific catabolite repression-resistant mutants of ... Putative contribution of glucose kinase from Bacillus subtilis to carbon catabolite repression (CCR): a link between enzymatic ... and the HPr kinase leading to carbon catabolite repression [9]. The glucose-transporting PTS, which is bacterial specific, ... transcription repression complex, and (d) protein import complex [24]. Based on this functional deduction, the C-terminus ...
A role for carbon catabolite repression in the metabolism of phosphonoacetate by Agromyces fucosus Vs2. OLoughlin, SN., Graham ...
Catabolite Repression G6.590.235 G3.194 G5.308.48 G6.173 Catalysis G2.149.170 G2.130 G2.149.767.500 G2.842.750.500 Catalytic ... Enzyme Repression G5.355.315.320.300 G5.308.320.300 Eosine I Bluish D3.494.953.275.300 D3.633.300.953.275.300 Eosine Yellowish ... YS) D3.494.953.275.325 D3.633.300.953.275.325 Epigenesis, Genetic G5.355.315.203 G5.308.203 Epigenetic Repression G5.355. ...
  • Is T7 expression subject to catabolite repression in NEB T7 Express or SHuffle strains? (neb.com)
  • Inducibility of the TOL catabolic pathways in Pseudomonas putida (pWWO) growing on succinate in continuous culture : evidence of carbon catabolite repression control. (microbiologyresearch.org)
  • E. coli are unable to uptake lactose in the presence of glucose, a phenomenon known as carbon catabolite repression. (igem.org)
  • Background Carbon catabolite repression (CCR) controls the order in which different carbon sources are metabolised. (inria.fr)
  • Specifically, sugar mixtures are often incompletely metabolized due to carbon catabolite repression while composition variability further complicates the optimization of co-utilization rates. (elsevier.com)
  • We show that alpha-glucan degrading activity dissipates when Lactobacillus crispatus is grown on glucose, maltose and maltotriose, in agreement with carbon catabolite repression elements flanking the pulA gene. (bvsalud.org)
  • Catabolite repression in the D-serine deaminase system of Escherichia coli K-12. (nih.gov)
  • Catabolite repression in Escherichia Coli - a comparison of modelling approaches. (mpg.de)
  • A quantitative approach to catabolite repression in Escherichia coli. (mpg.de)
  • Regulatory, loss of catabolite repression: growth on maltose + a-methylgucoside. (yale.edu)
  • Since expression is controlled by the wt lac operon, glucose addition will result in catabolite repression. (neb.com)
  • Catabolite repression is mediated by Enzyme IIA Glucose (IIAGluc), which inhibits the uptake of lactose in the presence of glucose by binding to LacY 4 . (igem.org)
  • Process by which micro-organisms adapt quickly to a preferred rapidly-metabolizable intermediate through the inhibition or repression of genes related to CATABOLISM of less preferred source(s). (bvsalud.org)
  • This repression was not dependent on either the fnr or resDE gene products, which have been shown to regulate expression of other B. subtilis genes in response to anaerobic conditions. (elsevier.com)
  • Additionally, catabolite control proteins CcpA and CcpB were not responsible for the repression. (elsevier.com)
  • A dyad symmetry element located between positions -73 and -59 relative to the transcription start site of the aconitase gene (citB) promoter was previously shown to be a target of catabolite repression and the binding site for a putative negative regulator during aerobic growth. (elsevier.com)
  • The deletion of the upstream arm of the dyad symmetry region abolished the citB repression observed during anaerobic growth. (elsevier.com)
  • This nitrogen-response system, often referred to as nitrogen catabolite repression, is found in many fungi and bacteria. (nih.gov)
  • Nitrogen metabolite repression in Aspergillus nidulans . (zju.edu.cn)
  • Link to all annotated objects annotated to nitrogen catabolite regulation of transcription. (cyverse.org)
  • Link to all direct and indirect annotations to nitrogen catabolite regulation of transcription. (cyverse.org)
  • however, repression could be relieved completely when 4-aminobutyrate (GABA) served as the sole nitrogen source. (unipr.it)
  • In this study the role of Crc in catabolite repression control has been studied in Pseudomonas putida. (uncg.edu)
  • Effect of vfr mutation on global gene expression and catabolite repression control of Pseudomonas aeruginosa. (jefferson.edu)
  • We identified a putative catabolite-responsive element (cre) in the promoter regions of all known tst genes, indicating that tst transcription may be regulated by the catabolite control protein CcpA. (nih.gov)
  • chaperone Hfq, the catabolite repression control protein Crc and the regulatory RNA CrcZ (1,7). (biologyexperimentideas.net)
  • Catabolite repression in Escherichia coli mutants lacking cyclic AMP receptor protein. (nih.gov)
  • Cyclic AMP receptor protein was originally identified as a catabolite gene activator protein. (jefferson.edu)
  • Galinier A, Kravanja M, Engelmann R, Hengstenberg W, Kilhoffer MC, Deutscher J, Haiech J (1998) New protein kinase and protein phosphatase families mediate signal transduction in bacterial catabolite repression. (genome.jp)
  • La répression catabolique est étudiée sous différents aspects sur la bactérie phytopathogène modèle: du contrôle de l'expression de genes d'utilisation de la plante implicant le régulateur CRP (Catabolite Repression Protein) à des approches globales des réseaux de régulation chez les bactéries phytopathogènes. (insa-lyon.fr)
  • They have been phenotypically characterized and their ability to enhance the expression of catabolite-repressible operons has been tested. (nih.gov)
  • Theseobservations suggest that microorganisms use similar conserved domains tosense similar environmental signals and transmit this information viadifferent signal transduction pathways to different regulatory circuits:transcriptional regulation (histidine kinases), chemotaxis(methyl-accepting proteins), catabolite repression (adenylate cyclases),and modulation of enzyme activity (diguanylate cyclases andphosphodiesterases). (embl.de)
  • This is the first report of a successful catabolite repression insensitivity of cellulase production by a mutant of B. pumilus, and is particularly significant when compared to Trichoderma reesei a well-known cellulase producer, which is under control of end-product inhibition. (semanticscholar.org)
  • Catabolite repression in Campylobacter jejuni correlates with intracellular succinate levels. (bio-protocol.org)
  • cAMP, or cyclic adenosine monophosphate -- A form of AMP, generated from ATP by adenylyl cyclase in response to the stimulation of many types of cell-surface receptors, used frequently as a second messenger in eukaryotics (cells containing one nucleus) and in catabolite repression in prokaryotes (cells containing no nucleus). (nih.gov)
  • Om du har frågor, behöver hjälp, hittar en bugg eller vill ge feedback kan du göra det här nedan. (chalmers.se)
  • FOG-1-dependent repression can be accompanied by broad histone deacetylation (Grass et al. (booksc.org)