A common, benign, usually self-limited viral infection of the skin and occasionally the conjunctivae by a poxvirus (MOLLUSCUM CONTAGIOSUM VIRUS). (Dorland, 27th ed)
A species of MOLLUSCIPOXVIRUS causing skin lesions in humans. It is transmitted by direct contact or from non-living reservoirs (fomites), such as books or clothing.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Primary immunodeficiency syndrome characterized by recurrent infections and hyperimmunoglobulinemia E. Most cases are sporadic. Of the rare familial forms, the dominantly inherited subtype has additional connective tissue, dental and skeletal involvement that the recessive type does not share.
A dysgammaglobulinemia characterized by a deficiency of IMMUNOGLOBULIN G.
Cell surface receptors that bind TUMOR NECROSIS FACTORS and trigger changes which influence the behavior of cells.
A condition produced by dietary or metabolic deficiency. The term includes all diseases caused by an insufficient supply of essential nutrients, i.e., protein (or amino acids), vitamins, and minerals. It also includes an inadequacy of calories. (From Dorland, 27th ed; Stedman, 25th ed)
The state of being free from intrusion or disturbance in one's private life or affairs. (Random House Unabridged Dictionary, 2d ed, 1993)
Customer satisfaction or dissatisfaction with a benefit or service received.
The privacy of information and its protection against unauthorized disclosure.
The protection of genetic information about an individual, family, or population group, from unauthorized disclosure.
Facilities for the preparation and dispensing of drugs.
The interchange of goods or commodities, especially on a large scale, between different countries or between populations within the same country. It includes trade (the buying, selling, or exchanging of commodities, whether wholesale or retail) and business (the purchase and sale of goods to make a profit). (From Random House Unabridged Dictionary, 2d ed, p411, p2005 & p283)
Disorders characterized by abnormal proliferation of primary cells of the immune system or by excessive production of immunoglobulins.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.
Progenitor cells from which all blood cells derive.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Antibodies produced by a single clone of cells.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.
Chemicals administered to patients in order to diagnose or study the pathology of medical conditions, diseases, or syndromes.
Inorganic compounds that contain ruthenium as an integral part of the molecule.
A way of providing nursing care that is guided by the integration of the best available scientific knowledge with nursing expertise. This approach requires nurses to critically assess relevant scientific data or research evidence, and to implement high-quality interventions for their nursing practice.
Research carried out by nurses, generally in clinical settings, in the areas of clinical practice, evaluation, nursing education, nursing administration, and methodology.
An agency of the PUBLIC HEALTH SERVICE concerned with the overall planning, promoting, and administering of programs pertaining to maintaining standards of quality of foods, drugs, therapeutic devices, etc.
An oath, attributed to Hippocrates, that serves as an ethical guide for the medical profession.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.

MycN sensitizes neuroblastoma cells for drug-induced apoptosis. (1/2199)

Amplification of the MYCN gene is found in a large proportion of neuroblastoma and considered as an adverse prognostic factor. To investigate the effect of ectopic MycN expression on the susceptibility of neuroblastoma cells to cytotoxic drugs we used a human neuroblastoma cell line harboring tetracycline-controlled expression of MycN. Neither conditional expression of MycN alone nor low drug concentrations triggered apoptosis. However, when acting in concert, MycN and cytotoxic drugs efficiently induced cell death. Apoptosis depended on mitochondrial permeability transition and activation of caspases, since the mitochondrion-specific inhibitor bongkrekic acid and the caspase inhibitor zVAD-fmk almost completely abrogated apoptosis. Loss of mitochondrial transmembrane potential and release of cytochrome c from mitochondria preceded activation of caspase-8 and caspase-3 and cleavage of PARP. CD95 expression was upregulated by treatment with cytotoxic drugs, while MycN cooperated with cytotoxic drugs to increase sensitivity to CD95-induced apoptosis and enhancing CD95-L expression. MycN overexpression and cytotoxic drugs also synergized to induce p53 and Bax protein expression, while Bcl-2 and Bcl-X(L) protein levels remained unchanged. Since amplification of MYCN is usually associated with a poor prognosis, these findings suggest that dysfunctions in apoptosis pathways may be a mechanism by which MycN-induced apoptosis of neuroblastoma cells is inhibited.  (+info)

Tumor necrosis factor alpha regulation of the FAS-mediated apoptosis-signaling pathway in synovial cells. (2/2199)

OBJECTIVE: Fas-mediated apoptosis is observed in synoviocytes of patients with rheumatoid arthritis (RA), but not in those of patients with osteoarthritis (OA). The present study was conducted to elucidate the mechanisms that initiate induction of Fas-mediated apoptosis in RA synoviocytes. METHODS: Cultured OA synoviocytes, which are insensitive to Fas-mediated apoptosis in spite of Fas antigen expression, were used in these experiments. Synovial cell proliferation and cytotoxicity studies were performed using MTS and lactate dehydrogenase release assays. Surface expression of Fas antigen was analyzed by flow cytometry. The expression and function of apoptosis-signaling molecules, such as caspase 8 and caspase 3, were examined by immunoblot analysis. RESULTS: Tumor necrosis factor alpha (TNFalpha) induced proliferation of cultured OA synoviocytes. Fas ligation with anti-Fas monoclonal antibody (mAb) resulted in cytotoxic activity against cultured OA synoviocytes that had been pretreated with TNFalpha for 5 days, but not those pretreated for 2 days. In contrast, anti-Fas mAb did not show a cytotoxic effect against untreated cultured OA synoviocytes. A gradual up-regulation of caspase 8 and caspase 3, which played a role in the caspase cascade for Fas-mediated apoptosis, was observed in TNFalpha-treated cultured OA synoviocytes. In addition, Fas ligation to TNFalpha-treated cultured OA synoviocytes induced activation of caspase 8 and caspase 3, with subsequent cleavage of poly(ADP-ribose) polymerase (PARP), a substrate of activated caspase 3. More importantly, Z-IETD-FMK, a caspase 8 inhibitor, and Ac-DEVD-CHO, a caspase 3 inhibitor, almost completely inhibited Fas-mediated apoptosis of TNFalpha-treated cultured OA synoviocytes, whereas Ac-YVAD-CHO, a caspase 1 inhibitor, did not. CONCLUSION: Our results clearly demonstrate that TNFalpha stimulates synovial cells to proliferate as well as sensitizes the cells for Fas-mediated apoptosis, at least in part by up-regulation and activation of caspase 8 and caspase 3. These findings suggest that TNFalpha may be one of the factors providing sensitization of synovial cells to Fas-mediated apoptosis in RA.  (+info)

Solution structure of BID, an intracellular amplifier of apoptotic signaling. (3/2199)

We report the solution structure of BID, an intracellular cross-talk agent that can amplify FAS/TNF apoptotic signal through the mitochondria death pathway after Caspase 8 cleavage. BID contains eight alpha helices where two central hydrophobic helices are surrounded by six amphipathic ones. The fold resembles poreforming bacterial toxins and shows similarity to BCL-XL although sequence homology to BCL-XL is limited to the 16-residue BH3 domain. Furthermore, we modeled a complex of BCL-XL and BID by aligning the BID and BAK BH3 motifs in the known BCL-XL-BAK BH3 complex. Additionally, we show that the overall structure of BID is preserved after cleavage by Caspase 8. We propose that BID has both BH3 domain-dependent and -independent modes of action in inducing mitochondrial damage.  (+info)

Solution structure of the proapoptotic molecule BID: a structural basis for apoptotic agonists and antagonists. (4/2199)

Members of the BCL2 family of proteins are key regulators of programmed cell death, acting either as apoptotic agonists or antagonists. Here we describe the solution structure of BID, presenting the structure of a proapoptotic BCL2 family member. An analysis of sequence/structure of BCL2 family members allows us to define a structural superfamily, which has implications for general mechanisms for regulating proapoptotic activity. It appears two criteria must be met for proapoptotic function within the BCL2 family: targeting of molecules to intracellular membranes, and exposure of the BH3 death domain. BID's activity is regulated by a Caspase 8-mediated cleavage event, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, resulting in a change of cellular localization.  (+info)

Targeted disruption of caspase genes in mice: what they tell us about the functions of individual caspases in apoptosis. (5/2199)

Cysteine proteases of the caspase family are crucial mediators of apoptosis. All mammalian cells contain a large number of caspases. Although many caspases are activated in a cell committed to apoptosis, recent data from caspase gene knockout mice suggest that individual caspases may be involved in the cell and stimulus-specific pathways of cell death. The gene disruption studies also establish the functional hierarchy between two structurally distinct classes of caspases. The present review discusses these recent findings and elaborates on how these mutant mouse models have helped the understanding of the mechanisms that govern programmed cell death in the immune and other systems.  (+info)

Ordering of ceramide formation, caspase activation, and mitochondrial changes during CD95- and DNA damage-induced apoptosis. (6/2199)

To evaluate the role of ceramide (Cer) in apoptosis signaling, we examined Cer formation induced by CD95, etoposide, or gamma-radiation (IR) in relation to caspase activation and mitochondrial changes in Jurkat T cells. The Cer response to all three stimuli was mapped in between caspases sensitive to benzoyloxycarbonyl-VAD-fluoromethylketone (zVAD-fmk) and acetyl-DEVD-aldehyde (DEVD-CHO). Cer production was independent of nuclear fragmentation but associated with the occurrence of other aspects of the apoptotic morphology. Caspase-8 inhibition abrogated Cer formation and apoptosis induced by CD95 but did not affect the response to etoposide or IR, placing CD95-induced Cer formation downstream from caspase-8 and excluding a role for caspase-8 in the DNA damage pathways. CD95 signaling to the mitochondria required caspase-8, whereas cytochrome c release in response to DNA damage was caspase-independent. These results indicate that the caspases required for the Cer response to etoposide and IR reside at or downstream from the mitochondria. Bcl-2 overexpression abrogated the Cer response to etoposide and IR and reduced CD95-induced Cer accumulation. We conclude that the Cer response to DNA damage fully depends on mitochondrion-dependent caspases, whereas the response to CD95 partially relies on these caspases. Our data imply that Cer is not instrumental in the activation of inducer caspases or signaling to the mitochondria. Rather, Cer formation is associated with the execution phase of apoptosis.  (+info)

Caspase-8 is required for cell death induced by expanded polyglutamine repeats. (7/2199)

We show here that caspase-8 is required for the death of primary rat neurons induced by an expanded polyglutamine repeat (Q79). Expression of Q79 recruited and activated caspase-8. Inhibition of caspase-8 blocked polyglutamine-induced cell death. Coexpression of Q79 with the caspase inhibitor CrmA, a dominant-negative mutant of FADD (FADD DN), Bcl-2, or Bcl-xL, but not an N-terminally tagged Bcl-xL, prevented the recruitment of caspase-8 and inhibited polyglutamine-induced cell death. Furthermore, Western blot analysis revealed the presence of activated caspase-8 in the insoluble fraction of affected brain regions from Huntington's disease (HD) patients but not in those from neurologically unremarkable controls, suggesting the relocation and activation of caspase-8 during the pathogenesis of HD. These results suggest an essential role of caspase-8 in HD-related neural degenerative diseases.  (+info)

Cell death attenuation by 'Usurpin', a mammalian DED-caspase homologue that precludes caspase-8 recruitment and activation by the CD-95 (Fas, APO-1) receptor complex. (8/2199)

Apoptotic cell suicide initiated by ligation of CD95 (Fas/APO-1) occurs through recruitment, oligomerization and autocatalytic activation of the cysteine protease, caspase-8 (MACH, FLICE, Mch5). An endogenous mammalian regulator of this process, named Usurpin, has been identified (aliases for Usurpin include CASH, Casper, CLARP, FLAME-1, FLIP, I-FLICE and MRIT). This protein is ubiquitously expressed and exists as at least three isoforms arising by alternative mRNA splicing. The Usurpin gene is comprised of 13 exons and is clustered within approximately 200 Kb with the caspase-8 and -10 genes on human chromosome 2q33-34. The Usurpin polypeptide has features in common with pro-caspase-8 and -10, including tandem 'death effector domains' on the N-terminus of a large subunit/small subunit caspase-like domain, but it lacks key residues that are necessary for caspase proteolytic activity, including the His and Cys which form the catalytic substrates diad, and residues that stabilize the P1 aspartic acid in substrates. Retro-mutation of these residues to functional caspase counterparts failed to restore proteolytic activity, indicating that other determinants also ensure the absence of catalytic potential. Usurpin heterodimerized with pro-caspase-8 in vitro and precluded pro-caspase-8 recruitment by the FADD/MORT1 adapter protein. Cell death induced by CD95 (Fas/APO-1) ligation was attenuated in cells transfected with Usurpin. In vivo, a Usurpin deficit was found in cardiac infarcts where TUNEL-positive myocytes and active caspase-3 expression were prominent following ischemia/reperfusion injury. In contrast, abundant Usurpin expression (and a caspase-3 deficit) occurred in surrounding unaffected cardiac tissue, suggesting reciprocal regulation of these pro- and anti-apoptotic molecules in vivo. Usurpin thus appears to be an endogenous modulator of apoptosis sensitivity in mammalian cells, including the susceptibility of cardiac myocytes to apoptotic death following ischemia/ reperfusion injury.  (+info)

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The illustration depicts a wild type mouse and a littermate mutant mouse expressing a constitutive serine phosphorylation mutation in Fas-Associated Death Domain (FADD-D). FADD-D mice exhibit reduced body size. The corresponding confocal images show mislocalization of FADD-D protein. Activated T cells from either wild type or FADD-D mice were stained with FADD antibody (green
Active Caspase 3 FITC Staining Kit(Caspase 3FITC染色试剂盒)(ab65613)在活细胞中检测激活型caspase 3,基于流式、显微镜或荧光读板仪。
Active Caspase 2 FITC Staining Kit (ab65612). Active caspase 2 detection in living cells by flow, microscopy or fluorescent plate reader.
Death receptor (DR) ligation can lead to divergent signaling pathways causing either caspase-mediated cell death or cell proliferation and inflammation. These variations in cellular fate are determined by adaptor proteins that are recruited to the DR signaling complex. FLICE inhibitory protein (FLIP) is an established inhibitor of caspase-8-mediated apoptosis, and it is also involved in NF-kappa B
Induction of apoptosis is the most studied cell death process and it is a tightly regulated physiological event that enables elimination of damaged and unwanted cells. Apoptosis can be induced via activation of either the intrinsic or the extrinsic signalling pathway. The intrinsic pathway involves activation of the mitochondria by stress stimuli, whereas the extrinsic pathway is triggered by ligand induced activation of death receptors such as Fas. Apoptosis induction via Fas activation plays an important role in the function of cytotoxic T lymphocytes and in the control of immune cell homeostasis.. Several studies have shown that anticancer therapies require functional cell death signalling pathways. Irradiation based therapy has been successful in treatment of several malignancies but the usage of high doses has been associated with side effects. Therefore, low dose therapies, that either is optimized for specific delivery or administrated in combination with other treatments, are promising ...
CASP10; MCH4; Caspase-10; CASP-10; Apoptotic protease Mch-4; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE2; ICE-like apoptotic protease ...
RTN3 can recruit Fas-associated death domain (FADD), thus initiating the ER-stress activated apoptosis. It also interacts with the β-secretase and its aggregation is critically associated with Alzheimers disease. Here, we first investigated the solution conformation of hRTN3, subsequently characterized its binding with hFADD. The results reveal: (1) both hRTN3 N- and C-termini are intrinsically unstructured. Nevertheless, the C-terminus contains two short helix-populated regions. (2) The unstructured hRTN3 C-terminus can bind to hFADD as shown by ITC. Further NMR investigation successfully identified the binding involved hRTN3 residues. (3) Although upon hRTN3-binding, the perturbed hFADD residues were distributed over the whole sequence, the majority of the significantly perturbed are over its death effector domain, very different from the previously observed binding mode for FADD. This study also implies a possible linkage between Alzheimers disease and ER-stress activated apoptosis. © ...
SWISS-MODEL Repository entry for Q297U0 (FADD_DROPS), Fas-associated death domain protein. Drosophila pseudoobscura pseudoobscura (Fruit fly)
Yan, Q., McDonald, J. M., Zhou, T. and Song, Y. (2013), Structural insight for the roles of fas death domain binding to fadd and oligomerization degree of the fas-fadd complex in the death-inducing signaling complex formation: A computational study. Proteins, 81: 377-385. doi: 10.1002/prot.24193 ...
Effects of ponicidin on the activity of caspase-3 in MKN28 cells. After treatment, the activity of caspase-3 in MKN28 cells was analyzed by the caspase-3 assay
Caspase-9兔多克隆抗体(ab52298)可与小鼠, 大鼠样本反应并经ELISA, IHC, ICC/IF实验严格验证,被6篇文献引用。所有产品均提供质保服务,中国75%以上现货。
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A bid given to the balancing power market to increase generation or reduce consumption. When the bid is activated, the party carrying out the regulation sells regulating power to Fingrid.. ...
The death receptor (DR)-mediated apoptosis pathway is thought to be unique to vertebrates. However, the presence of DR-encoding genes in the sea urchin and the basal chordate amphioxus prompted us to reconsider, especially given that amphioxus contains 14 DR proteins and hundreds of death domain (DD)-containing adaptor proteins. To understand how the extrinsic apoptotic pathway was originally established and what the differences in signaling are between invertebrates and vertebrates, we performed functional studies of several genes that encode DDs in the amphioxus Branchiostoma belcheri tsingtauense (Bbt). First, we observed that the increased abundance of Bbt Fas-associated death domain 1 (BbtFADD1) in HeLa cells resulted in the formation of death effector filamentous structures in the cytoplasm and the activation of the nuclear factor κB pathway, whereas BbtFADD2 protein was restricted to the nucleus, although its death effector domain induced apoptosis when in the cytoplasm. We further ...
TY - JOUR. T1 - Hypericin induced death receptor-mediated apoptosis in photoactivated tumor cells.. AU - Ali, Seyed Mohamed. AU - Chee, Soo Khee. AU - Yuen, Gan Yik. AU - Olivo, Malini. PY - 2002/6. Y1 - 2002/6. N2 - Nasopharyngeal carcinoma (NPC) is a malignant disease of the head/neck region with a 5-year survival level of approximately 65%. To explore the novel therapeutic strategies in the management of this disease, the potential effects of photodynamic therapy (PDT) in NPC cells were investigated. PDT, a new mode of treatment, is based on the combined use of light-absorbing compounds and light irradiation. Two human NPC cells such as, poorly differentiated (NPC/CNE2) and moderately differentiated (NPC/TW0-1) and other types of tumor cells like colon (CCL-220.1) and bladder (SD) undergo rapid apoptosis when treated with PDT sensitized with hypericin (HY). It has been shown that this compound has a strong photodynamic effect on tumors and viruses. However, the initiating events of PDT ...
Thus, caspase-8 has a crucial pro-survival role in shutting off RIPK1 and preventing it from inducing necroptosis. But how, then, does a cell wherein caspase-8 is activated not die by apoptosis instead? How does it live to develop into a healthy mouse or human? Caspase-8 activates through dimerization; two molecules of caspase-8 are forcefully brought together to form an active complex. The previously mentioned adapter protein FADD is essential for initiating this process of dimerization, but recent evidence has shown that once a few dimers are formed around clusters of FADD, more caspase-8 dimers can form independent of FADD. An important clue comes from the observation that caspase-8 does not only activate when it dimerises with itself to form a homodimer, but can also when it forms a dimer with its cousin, FLIP (FLICE-like Inhibitory Protein), to form a heterodimer. FLIP is similar to caspase-8 but has no protease activity, it is an inactive caspase homologue. The heterodimer is active, but ...
Activation and Inhibition of ApoptosisSeveral mechanisms have been identified in mammalian cells for the induction of apoptosis. These mechanisms include factors that lead to perturbation of the mitochondria leading to leakage of cytochrome c or factors that directly activate members of the death receptor family. Fas is a member of the tumor necrosis factor (TNF) receptor superfamily, a family of transmembrane receptors that include neurotrophin receptor (p75NTR), TNF-R1, and a variety of other cell surface receptors. Fas Ligand (Fas L) transmits signals to Fas on a target cell by inducing trimerization of Fas. Activation of Fas causes the recruitment of Fas-associated protein with death domain (FADD) via interactions between the death domain of Fas and FADD and is followed by pro-caspase-8 binding to FADD via interactions between the death effector domains (DED) of FADD and pro-caspase-8 leading to the activation of caspase-8. Activation of caspase-8 leads to the activation of other
Lapatinib, a HER2/EGFR inhibitor, is the most recently approved targeted therapy for metastatic breast cancer. Based on clinical evidence that lapatinib enhances efficacy of capecitabine in breast cancer patients, we hypothesized that lapatinib could augment the anti-cancer activity of agents in other tumor types such as colon cancer. Using in vitro cell death and apoptosis assays, we found lapatinib to have minimal effect on chemotherapy-induced cytotoxicity in colon cancer cell lines. However, elevated concentrations of lapatinib (5-10μM) significantly enhanced the pro-apoptotic effects of TRAIL and agonistic TRAIL receptor antibodies. In vivo, xenografted colon tumors from mice treated with lapatinib/TRAIL combinations exhibited more immunostaining for cleaved caspase-8, an apical caspase in the extrinsic cell death pathway, compared to tumors from mice treated with lapatinib or TRAIL alone. Furthermore, the combination therapy suppressed tumor growth, whereas single agent treatments had ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Shop FAS-associated factor ELISA Kit, Recombinant Protein and FAS-associated factor Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Caspase detection antibodies and assays are used to help detect and study caspase activation in Apoptotic cells via immunoprecipitation and immunoblotting techniques.
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Antho 50 induces caspase 3 activation and UHRF1 down-regulation independently of p53 and p73.B CLL cells were incubated with Antho 50 at 75 μg/mL for the ind
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Studies of tumour necrosis factor receptor-1 in tumour necrosis factor receptor associated periodic sysndrome (TRAPS): United Kingdom
Evidence suggests that the signalling events which occur after apoptotic stimulation, define two basic mechanisms for the induction of apoptosis. The first is dependent on signalling via the mitochondria and the second is dependent upon signalling directly from the death receptors. After induction of apoptosis, there is a convergence in signalling at the level of caspase activation and subsequent biochemical and morphological changes. Therefore the efficacy of various inhibitors of apoptosis is dependent upon the initiating signal. In order to understand the apoptotic pathway, the mechanisms by which these inhibitors regulate chemical- and receptor-mediated apoptosis must be understood. The anti-apoptotic oncoprotein, Bcl-2, was shown to inhibit both staurosporine and Fas-mediated apoptosis in a manner which was partially dependent upon the level of Bcl-2 protein expressed. During both staurosporine and Fas-induced apoptosis Bcl-2 acted downstream of caspase-8 activation. High levels of Bcl-2 ...
Activation of Fas receptor by Fas ligand causes caspase 8 activation and apoptosis in cells and is an important mechanism by which normal tissue homeostasis and function are maintained. Activation of caspase 8 is preceded by the formation of a death-inducing signalling complex (DISC), and a number of redundant mechanisms regulate DISC formation in vivo. Fas receptor is widely expressed in tissues, and dysfunction of the regulatory mechanisms in Fas receptor signalling has been reported in several diseases including autoimmune disease and cancer. This review aims to identify and discuss the various mechanisms employed by cells to alter their sensitivity to Fas-mediated apoptosis by regulating DISC formation. We also discuss a number of defects identified with Fas receptor signalling and the associated pathologies.
Fingerprint Dive into the research topics of Se-Methyl-L-selenocysteine Induces Apoptosis via Endoplasmic Reticulum Stress and the Death Receptor Pathway in Human Colon Adenocarcinoma COLO 205 Cells. Together they form a unique fingerprint. ...
O15519: CASP8 and FADD-like apoptosis regulator; Caspase homolog; CASH; Caspase-eight-related protein; Casper; Caspase-like apoptosis regulatory protein; CLARP; Cellular FLICE-like inhibitory protein; c-FLIP; FADD-like antiapoptotic molecule 1; FLAME-1; Inhibitor of FLICE; I-FLICE; MACH-related inducer of toxicity; MRIT; Usurpin; CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12; ...
During the maturation process, spermatids must eliminate most of their cytoplasm to become small, highly motile sperm with their characteristic small DNA-packed head and tail for swimming. In fruit flies, caspases contribute to the elimination of the cytoplasm in a process called individualization, which begins in the head and ends with the elimination of the cytoplasmic contents into a waste bag from the tail end. Kaplan et al. identified Scotti (abbreviated soti), in a yeast two-hybrid screen for partners of the adaptor Klhl10, which is part of the E3 ligase complex required for individualization, and confirmed this interaction by coimmunoprecipitation from transfected S2 cells. Spermatids from soti-null mutant flies failed to mature, and immunostaining for active caspase showed that the abundance of activated caspase was abnormally high and that the developing spermatids failed to generate waste bags. In flies double homozygous for soti and cullin3 (cul3) or klhl10, caspase activation ...
Cellular caspase-8 (FLICE)-like inhibitory protein (cFLIP) was originally identified as an inhibitor of death-receptor signalling. However, this Review discusses new data indicating that cFLIP is also required for the survival and proliferation of T cells following T-cell-receptor stimulation. Cellular caspase-8 (FLICE)-like inhibitory protein (cFLIP) was originally identified as an inhibitor of death-receptor signalling through competition with caspase-8 for recruitment to FAS-associated via death domain (FADD). More recently, it has been determined that both cFLIP and caspase-8 are required for the
The IUPHAR/BPS Guide to Pharmacology. Caspase 1 - C14: Caspase. Detailed annotation on the structure, function, physiology, pharmacology and clinical relevance of drug targets.
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A long pro-domain caspase that has specificity for the precursor form of INTERLEUKIN-1BETA. It plays a role in INFLAMMATION by catalytically converting the inactive forms of CYTOKINES such as interleukin-1beta to their active, secreted form. Caspase 1 is referred as interleukin-1beta converting enzyme and is frequently abbreviated ICE ...
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Few things can be considered to be more important to a cell than its threshold for apoptotic cell death, which can be modulated up or down, but rarely in both directions, by a single enzyme. Therefore, it came as quite a surprise to find that one enzyme, glycogen synthase kinase-3 (GSK3), has the pe …
BioAssay record AID 306845 submitted by ChEMBL: Induction of apoptosis in U937 cells assessed as caspase 3 cleavage at 3 uM by Western blot.
The intracellular redox position is intently connected to the levels of professional-inflammatory cytokines, IL-1β, IL-23 and TNF-α which are the major factors of inflammatory responses. IFN-γ has also been shown to be linked with swelling although TNF-α has been researched thoroughly for its function in the inflammatory method and generation of ROS.Maintaining the earlier mentioned information into thought, we evaluated the level of IL-1β and IL-seventeen employing ELISA even though IFN-γ, IL-23 and TNF-α mRNA expression fold transform was identified utilizing qRT-PCR. We located that IL-1β ranges had been considerably larger in the two diabetic teams as in MEDChem Express 415903-37-6 contrast to the wholesome handle topics. The IL-1β is typically expressed by in-filtering macrophages, once activated they synthesize larger volume of nitric oxide as well. Curiously, there have been outstanding discrepancies in both NO and cytokine levels in the patients of higher age teams with glucose ...
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Caspase-7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Sequential activation
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Scientists at the NYU Langone Medical Center and the Laura and Isaac Perlmutter Cancer Center are researching a form of cancer treatment called necroptosis.
The Loss of Functional Caspase-12 in Europe Is a Pre-Neolithic Event. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
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TY - JOUR. T1 - Erratum. T2 - Human astrocytes are resistant to fas ligand and tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis (Journal of Neuroscience (March 22, 2006) (3299-3308)). AU - Song, Jin H.. AU - Bellail, Anita. AU - Tse, Margaret C.L.. AU - Yong, V. Wee. AU - Hao, Chunhai. PY - 2006/9/7. Y1 - 2006/9/7. UR - http://www.scopus.com/inward/record.url?scp=33748132489&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=33748132489&partnerID=8YFLogxK. M3 - Comment/debate. AN - SCOPUS:33748132489. VL - 26. JO - Journal of Neuroscience. JF - Journal of Neuroscience. SN - 0270-6474. IS - 18. ER - ...
Caspases are a conserved family of cysteine proteases. They play diverse roles in inflammatory responses and apoptotic pathways. Among the caspases is a subgroup whose primary function is to initiate apoptosis. Within their long prodomains, caspases-2, -9 and -12 contain a caspase activation and recruitment domain while caspases-8 and -10 bear death effector domains. Activation follows the recruitment of the procaspase molecule via the prodomain to a high molecular mass complex. Despite sharing some common features, other aspects of the biochemistry, substrate specificity, regulation and signaling mechanisms differ between initiator apoptotic caspases. Defects in expression or activity of these caspases are related to certain pathological conditions including neurodegenerative disorders, autoimmune diseases and cancer ...
758 Fas is a well characterized member of the death receptor family and its expression correlates with overall patient survival in Cholangiocarcinoma (Shimonishi et al;Hepatology 2000). Cellular FLICE-like inhibitory protein (c-FLIP) is an anti-apoptotic protein that blocks the activation of caspase 8 and apoptosis signaling through death inducing signaling complex (DISC). In our laboratory, we have established a unique Cholangiocarcinoma system with two cell populations that are sensitive (high surface Fas expression) or resistant (low surface Fas expression) to Fas-induced apoptosis (Pan et al; 1999; Am J Path). Fas sensitive, but not Fas resistant cells, undergo apoptosis when exposed to calmodulin (CaM) antagonists via a mechanism similar to Fas-induced death signaling. In an attempt to delineate the role of CaM in Fas mediated signaling, we previously demonstrated a direct interaction between CaM and Fas (Ahn et a; J Biol Chem. 2004) and that CaM is recruited to Fas-induced DISC ...
Caspase-1, originally called ICE, was the first mammalian analogue of the Caenorhabditis elegans death genes to be identified.11 Like all caspases, it is expressed as a proform, which is activated through proteolytic cleavage of an amino-terminal 11-kDa prodomain to release p20 and p10 subunits. Active caspase-1/ICE consists of a (p20/p10)2 tetramer, which is sufficient to process precursor IL-1β9,12-14 and, in at least some cell types, to induce apoptosis.16 Additionally, the prodomain has been postulated to have independent proapoptotic activity by enhancing death receptor-mediated caspase-8 activation.39 Although caspase-1 has conventionally been regarded as a proinflammatory, not a proapoptotic, caspase,10-12 it has been observed to induce or amplify apoptosis in tissue culture models.15,16. Regulated expression of caspase-1 in cardiac hypertrophy,17 the dilated cardiomyopathy of TNF-α overexpression,19 ischemia/infarction,21 and endotoxin-induced myocardial dysfunction18 prompted our ...
Caspase 3 antibody LS-C88630 is a biotin-conjugated rabbit polyclonal that binds human, mouse, rat, bovine, dog, hamster, pig, rabbit, and sheep caspase 3 (also known as CASP3). Caspase 3 antibody is validated for use in IHC-paraffin, immunoprecipitation, and western blot.
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This video will show you how to perform an apoptosis assay using adherent cells on the Celigo image cytometer using caspase 3/7 and Hoechst reagents.
Apoptosis or programmed death is a physiological process responsible for normal development and homeostasis of multicellular organisms. This process involves a well-functioning machinery of death, which are the main component of cysteine ​​proteases - caspases family. These enzymes are present in cells in a latent form and become activated during apoptosis induced by various factors. This review summarizes the progress made on structure, mechanism of activation, catalytic properties, are substrates of caspases and regulation of their activity. Also shown in the involvement of caspases in the major pathways of apoptosis.. ...
Read independent reviews on Caspase Fluorometric Substrate Set II Plus from AMS Biotechnology (Archived Products) on SelectScience
Human caspase 2 ELISA kit can be used for detecting in vitro quantitative levels of caspase-2 (CASP2) in human serum, cell culture supernatant, plasma, tissue
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|strong|Rabbit anti caspase-9 p10 antibody|/strong| recognizes caspase-9, a member of the cysteine-aspartic acid protease family. The active form of caspase-9 is generated by cleavage of the pro-caspa…
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Research tested and proven Caspase-10/b-Flice 2 antibody. Antibody is guaranteed to work in western blot applications and is reactive in human and mouse.
The major goal of this project is to understand how the negative regulation of necroptosis by RIPK1 and the positive regulation of necroptosis by RIPK3 affects hematopoieiss at steady state and following transplantation and infection. Aim 1b and Aim 3a have some overlap with this application ...
2003 nomenclature IA - Fas IB - Fas ligand IIA - Caspase 10 IIB - Caspase 8 III - unknown IV - Neuroblastoma RAS viral oncogene ... Fas and Fas-ligand interact to trigger the caspase cascade, leading to cell apoptosis. Patients with ALPS have a defect in this ... 20% of patients CEDS: Caspase 8 deficiency state. No longer considered a subtype of ALPS but distinct disorder RALD: NRAS, KRAS ... 3 reported cases ALPS-CASP10: Caspase 10. Germline CASP10 mutation. 2% of patients ALPS-U: Undefined. ...
Caspase-3, caspase-8, and caspase-9 have been reported to be modified by O-GlcNAc. Caspase-8 is modified near its cleavage/ ... Hyper-O-GlcNAcylation in PDAC cells appeared to be anti-apoptotic, inhibiting cleavage and activation of caspase-3 and caspase- ... deoxy-GlcNAc Reveals O-GlcNAc Modification of the Apoptotic Caspases That Can Block the Cleavage/Activation of Caspase-8". ... GlcNAc with 5S-GlcNAc accelerated caspase activation while pharmacological raising of O-GlcNAc with thiamet-G inhibited caspase ...
"The marine lipopeptide somocystinamide A triggers apoptosis via caspase 8". Proceedings of the National Academy of Sciences. ...
Hu WH, Johnson H, Shu HB (April 2000). "Activation of NF-kappaB by FADD, Casper, and caspase-8". J. Biol. Chem. 275 (15): 10838 ... In 2000, he and his colleagues showed that FADD, Casper (caspase-8-related protein), and caspase-8 play important roles in NF- ... They also cloned and identified a novel AIF-homologous molecule called AMID, which can lead the way to caspase-independent ... induces caspase-independent apoptosis". J. Biol. Chem. 277 (28): 25617-23. doi:10.1074/jbc.M202285200. PMID 11980907. Xu LG, ...
... the activation of caspase-9, caspase-3 and the decrease of Bcl-2. Those phenomena indicate the role of mitochondrial in the ... This can stimulate the apoptosis by activating downstream caspase-3 or by cleaving Bid. As a result, the cleavage of Bid (tBid ... Furthermore, the increase in caspase-8 activation is also observed. ... 148 (2): 203-8. doi:10.1007/s12011-012-9364-2. PMID 22367705. S2CID 16035022. Li GQ, Chen XG, Wu XP, Xie JD, Liang YJ, Zhao XQ ...
The caspase-independence of necroptosis allows the cell to bypass caspase activation, decreasing the time during which the ... in a caspase-independent fashion in the presence of viral caspase inhibitors to restrict virus replication. In addition to ... Conversely, caspase 8 inhibition of necroptosis can be bypassed by the necroptotic machinery through the anti-apoptotic protein ... The best characterized example of this co-regulation is the ability of caspase 8 to inhibit the formation of the necrosome by ...
"RIPK1 blocks early postnatal lethality mediated by caspase-8 and RIPK3". Cell. 157 (5): 1189-202. doi:10.1016/j.cell.2014.04. ...
"AID and Caspase 8 Shape the Germinal Center Response through Apoptosis". The Journal of Immunology. 191 (12): 5840-5847. doi: ...
APO-1-mediated apoptosis can be inhibited by a variety of factors, including the viral caspase inhibitors CrmA and p35, as well ... The DISC is composed of the death receptor, FADD, and caspase 8. It transduces a downstream signal cascade resulting in ... This proteolytic activity then results in a cascade of caspase activation, and ultimately cell death. This apoptotic activity ... but lack the amino acids necessary for caspase-8 catalytic activity. It is thought that c-FLIP may be involved in modulating ...
Genetic abnormalities frequently occur in a tumor-suppressor gene called caspase 8. Inactivation of this gene will result in ...
Xkr8 was activated directly by caspases and required a caspase-3 cleavage site for its function. CED-8, the only Caenorhabditis ... Chen, Yu-Zen; Mapes, James; Lee, Eui-Seung; Skeen-Gaar, Riley Robert; Xue, Ding (2013-01-01). "Caspase-mediated activation of ... Thus, there is a link between caspase activation and PS externalization, which triggers phagocytosis of apoptotic cells. Ho, ... is a substrate of the CED-3 caspase. Cleavage of CED-8 by CED-3 activates its proapoptotic function and generates a carboxyl- ...
A follow-up study researched to determine if the caspases were involved in the apoptosis seen in the previous study as well as ... The study confirmed that there was cleavage of caspase-3, -8, and -9. All three of these cysteine proteases play an important ... 8 (3): 343-52. PMID 9056677. Archived from the original on 2014-04-26. Thyrell L, Erickson S, Zhivotovsky B, et al. (February ... 21 (8): 1251-62. doi:10.1038/sj.onc.1205179. PMID 11850845. Peginterferon alfa-2b in the U.S. National Library of Medicine's ...
Caspase proteins are crucial mediators of apoptosis, with caspase-3 and caspase-8 being death proteases. Considering ... This is due to an increased expression of caspase‐3 and caspase8. ... RU, Porter AG and Jänicke (1999). "Emerging roles of caspase-3 in apoptosis. - PubMed - NCBI". Cell Death and Differentiation. ...
DRB1*04:06: caspase-8 autoantibodies silicosis-systemic sclerosis (SSc)-systemic lupus erythematosus (SLE). DRB1*04:09: T. ... Ueki A, Isozaki Y, Kusaka M (2005). "Anti-caspase-8 autoantibody response in silicosis patients is associated with HLA-DRB1, ... 51 (1): 23-8. doi:10.1136/ard.51.1.23. PMC 1004612. PMID 1371662. Hashimoto K, Maruyama H, Nishiyama M, Asaba K, Ikeda Y, Takao ... 8 (1): 103. doi:10.1186/ar1886. PMC 1526581. PMID 16542468. Winchester R (1981). "Genetic aspects of rheumatoid arthritis". ...
"TLR2 and caspase-8 are essential for group B Streptococcus-induced apoptosis in microglia". Journal of Immunology. 179 (9): ... 24 (8): 450-5. doi:10.1016/S0166-2236(00)01854-3. PMID 11476884. Barberà-Cremades M, Baroja-Mazo A, Gomez AI, Machado F, Di ... 36 (3): 613-8. doi:10.1161/01.STR.0000155729.12931.8f. PMID 15692122. Cardona AE, Pioro EP, Sasse ME, Kostenko V, Cardona SM, ... 28 (44): 11263-8. doi:10.1523/JNEUROSCI.2308-08.2008. PMC 6671487. PMID 18971468. Trang T, Beggs S, Wan X, Salter MW (March ...
There are two types of caspases: initiators and effectors. Initiator caspases cleave inactive forms of effector caspases. This ... This pathway controls the activation of caspase-9 by regulating the release of cytochrome c from the mitochondrial ... Caspases (cysteine-aspartic acid proteases) cleave at very specific amino acid residues. ... each leading to the activation of caspase-9. The nucleus and Golgi apparatus are other organelles that have damage sensors, ...
Kanneganti's lab showed compensatory roles for NLRP3/caspase-1 and caspase-8 in the regulation of IL-1β production in ... Her lab identified caspase-8 and FADD as expression and activation regulators of both the canonical and non-canonical NLRP3 ... This finding went against the dogma that existed at that time that caspase-8 and FADD were involved only in the apoptotic ... This study demonstrated that the NLRP3 inflammasome/pyroptotic pathway is closely connected to the caspase-8-mediated ...
Wei W, Norton DD, Wang X, Kusiak JW (2002). "Aβ 17-42 in Alzheimer's disease activates JNK and caspase8 leading to neuronal ...
Apoptosis Immune system Immune tolerance T-cell Autoimmunity Fas receptor Caspase Zhang J, Xu X, Liu Y. (2004), Activation- ... However, overexpression of the protein CFLAR (caspase and FADD-like apoptosis regulator) inhibits Fas-mediated apoptosis. ... Activated caspase 8 is released into the cytosol, where it activates the caspase cascade that initiates apoptosis. FasL is ... and proteolytically self-activates as caspase 8. Fas, FADD, and procaspase 8 together form a death-inducing signaling complex ( ...
"Phytosphingosine induces apoptotic cell death via caspase 8 activation and Bax translocation in human cancer cells". Clinical ...
The best characterized IAP is XIAP, which binds caspase-9, caspase-3 and caspase-7, thereby inhibiting their activation and ... caspases, numbered 3, 6, and 7. By inhibiting caspase 8, crmA prevents the other caspases from ever being activated. Inhibition ... By inhibiting caspase 1, also known as interleukin 1β converting enzyme (ICE), crmA prevents cytokines interleukin 1β from ... Regarding the activation of caspases, there exists a gene called ced-9 in C. elegans that protects against cell death that is a ...
... been known to regulate apoptosis by regulating the phosphorylation status of apoptotic proteins such as caspase-3 and caspase-8 ... "Ischemic preconditioning augments survival of stem cells via miR-210 expression by targeting caspase-8-associated protein 2". ... 42 (8): 493-499. doi:10.5483/BMBRep.2009.42.8.493. PMID 19712585. Zhang Z, Sun H, Dai H, Walsh RM, Imakura M, Schelter J, ... 8 (17): 2756-2768. doi:10.4161/cc.8.17.9387. PMID 19652553. Mizuno Y, Tokuzawa Y, Ninomiya Y, Yagi K, Yatsuka-Kanesaki Y, Suda ...
... that while caspase may play a role in apoptosis, it is specifically not as a result of caspase-3. It was reported in that study ... Caspase I is involved in the aforementioned cell membrane activity but not caspase-3. The sequence of events that leads to ... Reduction of potassium ions promotes apoptosis and the synthesis of initiator caspase-8 and the effector caspase-3. A study ... There are also differences in the initiation of mitochondrial (internal) and caspase-dependent (external) apoptosis for the UVC ...
... an investigational drug targeting caspases and caspase-like proteases: the clinical trials in sight and recent anti- ... May 2016). "The caspase-8 inhibitor emricasan combines with the SMAC mimetic birinapant to induce necroptosis and treat acute ... The substance acts as a pan-caspase inhibitor and has antiapoptotic and antiinflammatory effects. It was developed for the ... November 2005). "First-in-class pan caspase inhibitor developed for the treatment of liver disease". Journal of Medicinal ...
Henderson G, Peng W, Jin L, Perng GC, Nesburn AB, Wechsler SL, Jones C (December 2002). "Regulation of caspase 8- and caspase 9 ... Other research showed that the products from the first 4,658 nucleotides of LAT inhibited caspase-8 and caspase-9 cellular ... 8 (2): 103-111. doi:10.1080/13550280290101085. PMID 12491160. Wang QY, Zhou C, Johnson KE, Colgrove RC, Coen DM, Knipe DM (1 ... Further research has shown that HHV-8 LAT produces RNA which interfere not with expression of TGF-β1 and SMAD3, but reducing ...
It induces caspase-8 and caspase-9, which results in caspase-3 activation and poly(adp-ribose) polymerases cleavage. In 2002 it ... 8 (15): 1363-74. doi:10.2174/1381612023394610. PMID 12052213. Dalmazzo LF, Santana-Lemos BA, Jácomo RH, Garcia AB, Rego EM, da ... 10 (8): e1048052. doi:10.1080/15592324.2015.1048052. PMC 4623019. PMID 26179718. Whitehead, T. R.; Price, N. P.; Drake, H. L.; ... "1H-Indole-3-acetic acid" Registry of Toxic Effects of Chemical Substances (RTECS). Page last updated:November 8, 2017. "Indole- ...
It also selectively inhibits effector caspases 2, 3, 6, and 7 but not caspases 8 and 10. This compound has been shown to block ...
Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive ... Cowling V, Downward J (October 2002). "Caspase-6 is the direct activator of caspase-8 in the cytochrome c-induced apoptosis ... "Entrez Gene: CASP8 caspase 8, apoptosis-related cysteine peptidase".. *^ a b c Chun HJ, Zheng L, Ahmad M, Wang J, Speirs CK, ... Caspase 3. Pro-apoptotic:. BAX. BAK1/Bcl-2 homologous antagonist killer. Bcl-2-associated death promoter. Anti-apoptotic:. Bcl- ...
"Peptides from anchovy sauce induce apoptosis in a human lymphoma cell (U937) through the increase of caspase-3 and −8 ...
One novel protein (ProAgio) has been designed to bind at an unusual site, and then induces apoptosis by recruiting caspase 8. ... 21 (4): 383-8. doi:10.1038/nsmb.2797. PMC 4012256. PMID 24658351. integrin+alphaVbeta3 at the US National Library of Medicine ... "Milk fat globule EGF factor 8 in the serum of human patients of systemic lupus erythematosus". Journal of Leukocyte Biology. 83 ...
... of the presenilin 1/beta-catenin interaction and preservation of the heterodimeric presenilin 1 complex following caspase ... 2 (8). doi:10.1101/cshperspect.a006239. PMC 3405821. PMID 22908189.. *^ Su DM, Zhang Q, Wang X, He P, Zhu YJ, Zhao J, Rennert ... doi:10.1016/S0896-6273(01)00512-8. PMID 11719200.. *^ Buxbaum JD, Choi EK, Luo Y, Lilliehook C, Crowley AC, Merriam DE, Wasco W ... 279 (24): 25333-8. doi:10.1074/jbc.M312710200. PMID 15087467.. *^ Phiel CJ, Wilson CA, Lee VM, Klein PS (May 2003). "GSK-3alpha ...
CASP16P: encoding protein Caspase 16, pseudogene. *CCDC113: encoding protein Coiled-coil domain-containing protein 113 ...
... condensed apoptotic nuclei and a 2-4 fold increase in cortical precursors that stained immunopositive for cleaved caspase-3.[30 ... Brain-derived neurotrophic factor (BDNF), or abrineurin,[5] is a protein[6] that, in humans, is encoded by the BDNF gene.[7][8] ... 11 (8): 1169-80. doi:10.1017/S1461145708009309. PMID 18752720.. *^ Xiu MH, Hui L, Dang YF, Hou TD, Zhang CX, Zheng YL, Chen DC ... 8: 170. doi:10.3389/fncel.2014.00170. PMC 4064707. PMID 24999318.. *^ Heinonen I, Kalliokoski KK, Hannukainen JC, Duncker DJ, ...
... the Smac mimetic promotes formation of a RIPK1-dependent caspase-8-activating complex, leading to apoptosis. Recent studies ... 8 (3): 197-204. doi:10.1007/s10456-005-9010-0. PMID 16328160. Mercurio, Arthur M; Bachelder, Robin E; Bates, Richard C; Chung, ... STAT3 and RANTES contribute to the maintenance of drug resistance by upregulating anti-apoptotic signals and inhibiting caspase ...
The resulting deconstruction of cellular components is primarily carried out by specialized proteases known as caspases, but ... 278 (1): 311-8. doi:10.1074/jbc.M206279200. PMID 12401807.. *^ a b c Gille C, Goede A, Schlöetelburg C, Preissner R, Kloetzel ... 4 (8): 1015-8. doi:10.4161/cc.4.8.1900. PMID 16082197.. *^ a b Shringarpure R, Grune T, Mehlhase J, Davies KJ (January 2003). " ... 7 (8): 742-9. doi:10.1038/ncb0805-742. PMID 16056265.. *^ Sadanandom A, Bailey M, Ewan R, Lee J, Nelis S (October 2012). "The ...
HR has some similarities to animal pyroptosis, such as a requirement of caspase-1-like proteolytic activity of VPEγ, a cysteine ... November 2004). "VPEgamma exhibits a caspase-like activity that contributes to defense against pathogens". Current Biology. 14 ... When the cytoplasmic receptors MDA5 and RIG-I recognize a virus the conformation between the caspase-recruitment domain (CARD) ... 8 (4): 652-8. PMID 8152260.. *^ Klingemann HG (2010). "Development and testing of NK cell lines". In Lotze MT, Thompson AW (eds ...
Nevertheless, TRADD binds FADD, which then recruits the cysteine protease caspase-8. A high concentration of caspase-8 induces ... On the other hand, activated caspases cleave several components of the NF-κB pathway, including RIP, IKK, and the subunits of ... 17 (8): 884-886. doi:10.1096/fj.02-0670fje.. *^ Pedersen BK (December 2009). "The diseasome of physical inactivity - and the ... 60 (14): 756-8. doi:10.1007/BF01716573. PMID 6181289.. *^ Pennica D, Nedwin GE, Hayflick JS, Seeburg PH, Derynck R, Palladino ...
"Critical loss of CBP/p300 histone acetylase activity by caspase-6 during neurodegeneration". primary. The EMBO Journal. 22 (24 ... 82 (8): 850-2. doi:10.1136/jnnp.2009.200253. PMID 20551479.. *^ a b Swoboda KJ, Scott CB, Crawford TO, Simard LR, Reyna SP, ... 12 (8): 1261-8. doi:10.1002/pmic.201200010. PMID 22577027.. *^ Gräff J, Rei D, Guan JS, Wang WY, Seo J, Hennig KM, Nieland TJ, ... 19 (8): 1492-506. doi:10.1093/hmg/ddq023. PMID 20097677.. *^ Dewey CM, Cenik B, Sephton CF, Johnson BA, Herz J, Yu G (June 2012 ...
... to upregulate the activity of caspase-8. This causes cross talking of apoptotic signaling between caspase-8 and caspase-9 ... 39 (7-8): 1489-1499. doi:10.1016/j.biocel.2007.01.022. Quach, H; Ritchie, D; Stewart, A K; Neeson, P; Harrison, S; Smyth, M J; ... 79: 114-8. doi:10.1038/sj.bjc.6690020. PMC 2362163 . PMID 10408702. Bartlett, J. Blake; Dredge, Keith; Dalgleish, Angus G. (1 ... 87 (3): 503-8. doi:10.1111/j.1365-2141.1994.tb08304.x. PMID 7527645. Schafer, Peter (1 June 2012). "Apremilast mechanism of ...
... condensed apoptotic nuclei and a 2-4 fold increase in cortical precursors that stained immunopositive for cleaved caspase-3.[27 ... 11 (8): 1169-80. doi:10.1017/S1461145708009309. PMID 18752720.. *^ Xiu MH, Hui L, Dang YF, Hou TD, Zhang CX, Zheng YL, Chen DC ... 8: 170. doi:10.3389/fncel.2014.00170. PMC 4064707. PMID 24999318.. *^ Heinonen I, Kalliokoski KK, Hannukainen JC, Duncker DJ, ... 75 (8): e794-801. doi:10.4088/JCP.13m08772. PMID 25191916.. *^ Ray MT, Shannon Weickert C, Webster MJ (May 2014). "Decreased ...
Caspase 9 can then go on to activate caspase 3 and caspase 7, which are responsible for destroying the cell from within. ... Caspase 3. Pro-apoptotic:. BAX. BAK1/Bcl-2 homologous antagonist killer. Bcl-2-associated death promoter. Anti-apoptotic:. Bcl- ... Apoptosis & Caspase 3 - PMAP The Proteolysis Map-animation. *UMich Orientation of Proteins in Membranes families/superfamily-78 ... This release of cytochrome c in turn activates caspase 9, a cysteine protease. ...
Angiotensinogen · Caspase · F12 · Kimotripsinogen · Pepsinogen · Proelastase · Prokarboksipolipeptidase · Prolipase · ... 3 HSD · .4 · .5 · .6 · .7 · .8 · .9 · .10 · .11 · .12 · .13 · .14 · .15 · .16 · .17 · .18 · .19 · .20 · .21 · .22 · .23 · .24 · ... 1 PA · .2 · .3 · .4 · .5 · .6 · .7 · .8 · .9 · .10 · .11 · .12 · .13 · .14 · .15 · .16 · .17 · .18 · .19 · .20 · .21 · .22 α- ... 1 Rsk · .2 PDK · .3 · .4 · .5 · .6 · .7 · .8 · .9 · .10 · .11 · .12 · .13 · .14 · .15 · .16 · DAPK · .18 · .19 · .20 · .21 · . ...
This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2 ... and may be instrumental in the execution of apoptosis following caspase activation. However p21 may inhibit apoptosis and does ... 8: 14728. doi:10.1038/ncomms14728. PMID 28317845.. *^ Yu, Z.-K.; Gervais, J. L. M.; Zhang, H. (1998). "Human CUL-1 associates ... 437 (7062): 1173-8. doi:10.1038/nature04209. PMID 16189514.. *^ Frouin I, Maga G, Denegri M, Riva F, Savio M, Spadari S, ...
"Crocetin prevents retinal degeneration induced by oxidative and endoplasmic reticulum stresses via inhibition of caspase ... 2E,4E,6E,8E,10E,12E,14E)-2,6,11,15-Tetramethylhexadeca-2,4,6,8,10,12,14-heptaenedioic acid[2] ... InChI=1S/C20H24O4/c1-15(11-7-13-17(3)19(21)22)9-5-6-10-16(2)12-8-14-18(4)20(23)24/h5-14H,1-4H3,(H,21,22)(H,23,24)/b6-5+,11-7+, ... InChI=1/C20H24O4/c1-15(11-7-13-17(3)19(21)22)9-5-6-10-16(2)12-8-14-18(4)20(23)24/h5-14H,1-4H3,(H,21,22)(H,23,24)/b6-5+,11-7+,12 ...
"A novel form of DAP5 protein accumulates in apoptotic cells as a result of caspase cleavage and internal ribosome entry site- ... 273 (48): 31901-8. doi:10.1074/jbc.273.48.31901. PMID 9822659.. *^ Harris TE, Chi A, Shabanowitz J, Hunt DF, Rhoads RE, ... 273 (48): 31901-8. doi:10.1074/jbc.273.48.31901. PMID 9822659.. *. Ensinger C, Obrist P, Mikuz G, Merkx G, Smeets D, Bänziger R ...
Non obstante, a TRADD únese a FADD, o cal despois recruta a cisteína protease caspase-8. Unha alta concentración de caspase-8 ... Por outra parte, as caspases activadas clivan varios compoñentes da vía NF-κB, incluíndo a RIP, IKK, e as propias subunidades ... Wochenschr. 60 (14): 756-8. PMID 6181289. doi:10.1007/BF01716573.. *↑ Pennica D, Nedwin GE, Hayflick JS, Seeburg PH, Derynck R ... doi:10.1016/S0006-2952(03)00490-8.. *↑ Selwood T, Jaffe EK (2011). "Dynamic dissociating homo-oligomers and the control of ...
Excess progenitor cell proliferation that leads to gross brain abnormalities is often lethal, as seen in caspase-3 or caspase-9 ... Kuida, K (1998). "Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9". Cell. 94: 325-337 ... to cells (such as feedback from neighbors, stress or DNA damage), mitochondria release caspase activators that trigger the cell ... Kroemer G, Martin SJ (2005). "Caspase-independent cell death". Nature Medicine. 11 (7): 725-30. doi:10.1038/nm1263. PMID ...
Martinon F, Burns K, Tschopp J (2002). "The inflammasome: a molecular platform triggering activation of inflammatory caspases ... 8] 이 덕분에 망막 조직은 면역 특권 지역으로서 유지될 수 있다. 망막에서 다른 신체 부위처럼 면역 반응이 활성하면 망막 작용에 부정적인 영향이 크기 때문인데, [9] [10] [11] 이를 망막색소상피 세포가 외부 흐름을 ... "DICER1/Alu RNA dysmetabolism induces Caspase-8-mediated cell death in age-related macular degeneration". 》PNAS》 111 (45): 16082 ... Brian M. Kevany and Krzysztof Palczewski, Phagocytosis of Retinal Rod and Cone Photoreceptors, Physiology (Bethesda), 25(1): 8- ...
It is an energy dependent process mediated by proteolytic enzymes called caspases, which trigger cell death through the ... 8] Cytosolic components that leak through the damaged plasma membrane into the extracellular space can incur an inflammatory ...
Executioner Caspases (Caspase 3, Caspase 6 and Caspase 7). Once initiator caspases are activated, they produce a chain reaction ... Caspase-1, Caspase-4, Caspase-5 and Caspase-11 are considered 'Inflammatory Caspases'.[7] ... Caspase-1, Caspase-4 and Caspase-5 in humans, and Caspase-1 and Caspase-11 in mice play important roles in inducing cell death ... or direct activation of Executioner Caspases (Caspase 3, Caspase 6 and Caspase 7) to degrade cellular components as shown in ...
"Caspase 8 small interfering RNA prevents acute liver failure in mice". Proc Natl Acad Sci USA 100 (13): 7797-802. PMC 164667. ... ISBN 978-0-521-73444-8.. *↑ Matzke MA, Matzke AJM. (2004). "Planting the Seeds of a New Paradigm.". PLoS Biol 2 (5): e133. PMC ... doi:10.1016/S0168-9525(01)02367-8.. *↑ 66,0 66,1 Lucy A, Guo H, Li W, Ding S (2000). "Suppression of post-transcriptional gene ... doi:10.1007/s00425-003-1005-8.. *↑ Le L, Lorenz Y, Scheurer S, Fötisch K, Enrique E, Bartra J, Biemelt S, Vieths S, Sonnewald U ...
This complex then cleaves procaspase-9, activating caspase-9 and eventually inducing apoptosis via caspase-3 activation. Hsp70 ... 2 (8): 469-75. doi:10.1038/35019501. PMID 10934466.. *^ Gupta S, Deepti A, Deegan S, Lisbona F, Hetz C, Samali A (July 2010). ... The protein is then free to fold on its own, or to be transferred to other chaperones for further processing.[8] HOP (the Hsp70 ... 1 (1): 23-8. doi:10.1379/1466-1268(1996)001,0023:AHSGTT,2.3.CO;2. PMC 313013. PMID 9222585.. ...
Ubiquitin ligases transfer ubiquitin to its pendant, proteins, and caspases, which engage in proteolysis in the apoptotic cycle ... 61 (8-9). ISSN 0214-6282.. *^ Sekhar, Rajagopal V; Patel, Sanjeet G (2011). "Deficient synthesis of glutathione underlies ... 20 (8): 668. doi:10.1002/anie.198106681.. *^ Drauz, Karlheinz; Grayson, Ian; Kleemann, Axel; Krimmer, Hans-Peter; ... ISBN 0-8493-0462-8.. . *^ "Nomenclature and symbolism for amino acids and peptides (IUPAC-IUB Recommendations 1983)", Pure Appl ...
Caspase-independent apoptosis[edit]. The characterization of the caspases allowed the development of caspase inhibitors, which ... Caspases. Caspases play the central role in the transduction of ER apoptotic signals. Caspases are proteins that are highly ... The apoptosome cleaves the pro-caspase to its active form of caspase-9, which in turn cleaves and activates pro-caspase into ... There are two types of caspases: initiator caspases, caspase 2,8,9,10,11,12, and effector caspases, caspase 3,6,7. The ...
Stegh AH, Barnhart BC, Volkland J, Algeciras-Schimnich A, Ke N, Reed JC, Peter ME (Feb 2002). "Inactivation of caspase-8 on ... doi:10.1016/0092-8674(95)90530-8. PMID 7774010.. *^ Jiao Y, Lu Y, Li XY (Jan 2015). "Farnesoid X receptor: a master regulator ... 278 (22): 20420-8. doi:10.1074/jbc.M302505200. PMID 12660231.. *. Pircher PC, Kitto JL, Petrowski ML, Tangirala RK, Bischoff ED ... 284 (5418): 1365-8. doi:10.1126/science.284.5418.1365. PMID 10334993.. *. Bramlett KS, Yao S, Burris TP (Dec 2000). " ...
Caspase. *Caspase 1. *Caspase 2. *Caspase 3. *Caspase 4. *Caspase 5. *Caspase 6 ... 383 (7-8): 1285-9. doi:10.1515/BC.2002.144. PMID 12437118.. *. Wex T, Bühling F, Wex H, et al. (2001). "Human cathepsin W, a ... 167 (4): 2172-8. doi:10.4049/jimmunol.167.4.2172. PMID 11490002.. *. Wex T, Levy B, Wex H, Brömme D (2000). "Human cathepsins W ...
Also, it is extensively used in research for the detection of DNA damage,[34][35] caspase cleavage and apoptosis.[36] In ... Apoptosis (quantification, measurement of DNA degradation, mitochondrial membrane potential, permeability changes, caspase ... This process is called color compensation, which calculates a fluorochrome as a percentage to measure itself[8]. ...
Toisc go bhfuil an liosta bunaithe ar an leagan Béarla i 'Wikipedia en' beidh an leagan Béarla ar dtús agus an leagan Gaeilge ina dhiaidh. Tá súil agam, nuair atá méid áirithe den liosta aistrithe go Gaeilge gur féidir liosta i nGaeilge a chumadh nó i nGaeilge agus i mBéarla. ...
"Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9". Cell. 94 (3): 325-37. doi:10.1016/ ... 44 (2): 271-8. doi:10.1002/ajpa.1330440209. PMID 816206.. *^ Weiner, S; Monge, J; Mann, A (September 2008). "Bipedalism and ... 978-0-07-139011-8. .. *^ Smart, IH; McSherry, GM (June 1986). "Gyrus formation in the cerebral cortex in the ferret. I. ... Rakic, P (8 July 1988). "Specification of cerebral cortical areas". Science. 241 (4862): 170-6. Bibcode:1988Sci...241..170R. ...
"N-APP binds DR6 to cause axon pruning and neuron death via distinct caspases". Nature 457 (7232): 981-989. doi:10.1038/ ... doi:10.1007/s00018-003-3264-8 . PMID 15004691 . *↑ Hernández F, Avila J (Septemba 2007). "Tauopathies". Cell. Mol. Life Sci. 64 ... Neurol. 63 (1): 112-8. doi:10.1002/ana.21212. . PMID 18023012 . *Ikonomovic MD, Klunk WE, Abrahamson EE, et al. (Juni 2008). " ... Trans. 33 (Pt 2): 335-8. doi:10.1042/BST0330335 . PMID 15787600 . *↑ Ohnishi S, Takano K (Machi 2004). "Amyloid fibrils from ...
Caspase-8 deficiency (CEDS) is a very rare genetic disorder of the immune system. It is caused by mutations in the CASP8 gene ... Biochemically, caspase-8 was found to enter the complex of the inhibitor of NF-κB kinase (IKK) with the upstream Bcl10-MALT1 ( ... Caspase-8 is involved in the initiation of the cell death signal cascade. Cell death counters proliferation of lymphocytes, ... CEDS is caused by homozygous mutations in caspase-8. Caspase-8 is a 51 kb gene with 13 exons encoding for a 496 amino acid ...
Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive ... Cowling V, Downward J (October 2002). "Caspase-6 is the direct activator of caspase-8 in the cytochrome c-induced apoptosis ... "Entrez Gene: CASP8 caspase 8, apoptosis-related cysteine peptidase".. *^ a b c Chun HJ, Zheng L, Ahmad M, Wang J, Speirs CK, ... Caspase 3. Pro-apoptotic:. BAX. BAK1/Bcl-2 homologous antagonist killer. Bcl-2-associated death promoter. Anti-apoptotic:. Bcl- ...
To protect your privacy, your account will be locked after 6 failed attempts. After that, you will need to contact Customer Service to unlock your account.. You have 4 remaining attempts.. You have 3 remaining attempts.. You have 2 remaining attempts.. You have 1 remaining attempt.. Contact Customer Service ...
The caspase-deficient NB7 neuroblastoma cells have been described in ref. 34. Caspase-8-deficient and reconstituted Jurkat ... Caspase-3 (MAB4703, 1:500; Chemicon), caspase 9 (sc17784, 1:100; Santa Cruz Biotechnology), actin (1:5,000; Sigma), and PARP ( ... F) Similarly, the viability of neuroblastoma cultures deficient in caspase expression, or reconstituted for caspase 8 ... The marine lipopeptide somocystinamide A triggers apoptosis via caspase 8. Wolf Wrasidlo, Ainhoa Mielgo, Vicente A. Torres, ...
Pro-caspase-8 was easily detectable in an IP sample of pUL36AD169varDE diluted to the concentration that gave a similar pUL36 ... pUL36 Prevents Caspase-8 Activation.. Fas-mediated signaling results in caspase-8 being recruited to the Fas complex through an ... Pro-caspase-8 and FADD were detected by immunoblot analysis. Lanes designated L are samples of the transfected cell lysate ... C) Pro-caspase-8 fails to coimmunoprecipitate with pUL36AD169varATCC in transiently transfected 293T cells. Cells were ...
... rapidly induces cell death through recruitment and activation of caspase-8 via the adaptor protein Fas-associated death domain ... The caspase-8 inhibitor FLIP promotes activation of NF-κB and Erk signaling pathways. *Kataoka T ... there was activation of the transcription factors NF-κB and AP-1 and recruitment of the caspase-8 inhibitor and FADD- ... rapidly induces cell death through recruitment and activation of caspase-8 via the adaptor protein Fas-associated death domain ...
1). However, the dominant function of caspase 8 is to transmit a pro-survival signal that suppresses programmed necrosis (or ... Caspase 8 initiates apoptosis downstream of TNF death receptors by undergoing autocleavage and processing the executioner ... Caspase 8 inhibits programmed necrosis by processing CYLD Nat Cell Biol. 2011 Oct 30;13(12):1437-42. doi: 10.1038/ncb2362. ... Following TNF stimulation, caspase 8 cleaves CYLD to generate a survival signal. In contrast, loss of caspase 8 prevented CYLD ...
Ab12490 Caspase 3 siRNA vector ?ab12496 Caspase 8 siRNA vector ?ab12504 Caspase 9 siRNA vector ... I have now added the western blot image for the caspase 3 knockdown. It can be viewed on www.abcam.com/ab12490. If you do have ... I am interested in the SiRNA for Human Caspase-8. I will really apprciate if you send me the following information: 1) Is it ... Im interesting in your siRNA vectars against several caspases but there are no datas on your web pages. Could you show me the ...
Caspase-8 regulates TNF-α-induced epithelial necroptosis and terminal ileitis.. Günther C1, Martini E, Wittkopf N, Amann K, ... b) Western blot for RIP3 and cleaved caspase-3 of IEC lysates isolated from TNF-α treated control and Casp8ΔIEC mice. Actin ... e) Representative immunofluorescence staining for TUNEL and active caspase-3 in crypts of the terminal ileum of a CD-patient. ( ... Increased caspase-8 independent cell death within crypts of Casp8ΔIEC mice ...
Caspase-8 (Casp-8), a key protease in the extrinsic cell death-signaling pathway, regulates cell death via both apoptosis and ... 8 ECKO) in Casp-8ECKO/MLKLKO retinas compared with Casp-8WT/MLKLKO littermates. For B. , D. , F. , H. , and K. -. M. , data are ... EC-specific Casp-8-KO pups (Casp-8ECKO) showed reduced retina angiogenesis, as the loss of Casp-8 reduced EC proliferation, ... Representative images of the retinal vasculature stained with IsoB4 in Casp-8WT/MLKLKO and Casp-8ECKO/MLKLKO mice. Black arrows ...
... is the initiator caspase of the extrinsic apoptosis pathway that activates the effector caspase-3 (11). Cellular FLICE ... Caspase-8 serves both apoptotic and nonapoptotic roles. J Immunol. 2004;173(5):2976-2984.. View this article via: PubMed ... Caspase 8 inhibits programmed necrosis by processing CYLD. Nat Cell Biol. 2011;13(12):1437-1442.. View this article via: PubMed ... RIPK1 and caspase-8 ensure chromosome stability independently of their role in cell death and inflammation. Mol Cell. 2019;73(3 ...
Caspase-8. B. 271. Homo sapiens. Mutation(s): 2 Gene Names: CASP8, MCH5. EC: 3.4.22.61. ... Although widely regarded as an inhibitor of initiator caspase activation and cell death, c-FLIP(L) is also capable of enhancing ... Although widely regarded as an inhibitor of initiator caspase activation and cell death, c-FLIP(L) is also capable of enhancing ... Zymogen caspase-8:c-FLIPL protease domain complex. *DOI: 10.2210/pdb3H11/pdb ...
Mouse monoclonal Caspase-8 antibody [ABM14C1]. Validated in WB, IHC, Flow Cyt and tested in Human. Cited in 1 publication(s). ... All lanes : Anti-Caspase-8 antibody [ABM14C1] (ab220171) at 2 µg/ml. Lane 1 : Molt-4 cell lysate. Lane 2 : Kato III cell lysate ... Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell ... This product Mouse Anti-Caspase-8 antibody [ABM14C1] (ab220171) WB, Flow Cyt, IHC-P ...
... derived caspase substrates are widely used for the colorimetric detection of various caspase activities. Cleavage of pNA ... pNA has maximum absorption around 408 nm.; Caspase-8 substrate with Km = 66 uM; Substrate for granzyme B; Ac-IETD-pNA; Ac - Ile ... peptides by caspases generates pNA that is monitored colorimetrically at ~405 nm. ... pNA (4-nitroaniline)-derived caspase substrates are widely used for the colorimetric detection of various caspase activities. ...
Caspases were marked with FLICA reagents covalently marked with a fluorescent probe: FAM (6-fluorescein amidite) for caspase 8 ... Once inside the cell, the caspase inhibitors bind specifically through the peptide moiety to caspases that have been activated ... but only caspase 9 in SW620 cells [54]. After 48 hours, the two caspases were fully activated. Our results here emphasize the ... caspase 9) pathways are linked by protein Bid: activated caspase 8 splits Bid, which then later activates caspase 9. Both ...
... was due to increased cell death mediated by caspase-3. Coimmunoprecipitation studies revealed that caspase-8 was recruited to ... Caspase-8 is known to be important in regulating T cells. To study B cell-specific roles for caspase-8, Lemmers et al. ... Deletion of caspase-8 inhibited the increase in B cell numbers elicited by agonists for the Toll-like receptors (TLRs), TLR2, - ... Vital Nonapoptotic Functions for Caspase-8 in B Cells Message Subject. (Your Name) has forwarded a page to you from Science ...
2000 Jun 1;10(11):640-8. Research Support, Non-U.S. Govt; Research Support, U.S. Govt, P.H.S. ... The caspase-8 inhibitor FLIP promotes activation of NF-kappaB and Erk signaling pathways.. Kataoka T1, Budd RC, Holler N, Thome ... Activation of Fas (CD95) by its ligand (FasL) rapidly induces cell death through recruitment and activation of caspase-8 via ... there was activation of the transcription factors NF-kappaB and AP-1 and recruitment of the caspase-8 inhibitor and FADD- ...
Browse our Caspase-8 Peptides and Proteins all backed by our Guarantee+. ... Caspase-8 Peptides and Proteins available through Novus Biologicals. ... Our Caspase-8 Peptides and Caspase-8 Proteins can be used in a variety of model species: Human, Mouse. Use the list below to ... Each Caspase-8 Peptide and Caspase-8 Protein is fully covered by our Guarantee+, to give you complete peace of mind and the ...
J:55798 Imai Y, et al., The CED-4-homologous protein FLASH is involved in Fas-mediated activation of caspase-8 during apoptosis ...
Caspase expression and processing were analyzed by Western blotting using rat anti-mouse caspase-8 mAb (1G12; kindly donated by ... This difference might be attributable to the ability of caspase-10, a close homologue of caspase-8 that occurs in humans but ... Caspase-8 Serves Both Apoptotic and Nonapoptotic Roles. Tae-Bong Kang, Tehila Ben-Moshe, Eugene E. Varfolomeev, Yael Pewzner- ... Caspase-8 Serves Both Apoptotic and Nonapoptotic Roles. Tae-Bong Kang, Tehila Ben-Moshe, Eugene E. Varfolomeev, Yael Pewzner- ...
Ausgesuchte Qualitäts-Hersteller für Caspase 8 Antikörper. Hier bestellen. ... Monoklonale und polyklonale Caspase 8 Antikörper für viele Methoden. ... We observed that NDMP generation was dependent on the extrinsic caspase apoptotic pathway (caspase 3 and caspase 8), cAMP ... Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive ...
Caspase-8. A, B. 188. Homo sapiens. Mutation(s): 1 Gene Names: CASP8, MCH5. EC: 3.4.22.61. ... Structural basis for dimerization of the death effector domains of Caspase-8. Shen, C., Pei, J., Guo, X., Zhou, L., Li, Q., ... Structural basis for dimerization of the death effector domains of Caspase-8. *DOI: 10.2210/pdb5H33/pdb ...
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5: Entinostat enhances apoptosis in response to IAP antagonists via Ku70, RIPK1 and caspase-8.. From: Cytoplasmic FLIP(S) and ... 5: Entinostat enhances apoptosis in response to IAP antagonists via Ku70, RIPK1 and caspase-8. , Cell Death & Disease ... f Annexin-V/PI flow cytometry analysis of PC3 empty vector(EV) and procaspase-8(C8) CRISPR cells following pre-treatment for 24 ...
... Author(s). Hatting, M.; Zhao, G.; Schumacher, F. ... Caspase 8 (Casp8) is essential for death-receptor-mediated apoptosis activity and therefore its modulation might be critical ...
Proteintech Anti-Caspase 8 Polyclonal, Catalog # 13423-1-AP. Tested in Western Blot (WB), Immunofluorescence (IF), ... caspase) family. Sequential activation of caspases play a central role in the execution-phase of cell apoptosis. Caspases exist ... Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the pro-form of ... Caspase 8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death ...
Order monoclonal and polyclonal Caspase 8 antibodies for many applications. Selected quality suppliers for anti-Caspase 8 ... Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive ... anti-Caspase Recruitment Domain Family, Member 9 Antibodies * anti-Caspase Recruitment Domain-Containing Protein 18 (ICEBERG) ... anti-Caspase 7, Apoptosis-Related Cysteine Peptidase Antibodies * anti-Caspase 6, Apoptosis-Related Cysteine Peptidase ...
Caspase-8 is now known to have an undefined but integral function in lymphocyte activation and the phenotype of patients with ... Mutations in the caspase-8 gene not only affect apoptosis but also affect host defense. This article describes a heretofore ... Caspase-8 mutations lead to a disorder characterized by expansion of lymphocytes in secondary lymphoid organs and abnormal ... In addition, these patients had several features that appear to be specific for caspase-8 defects. The patients had diminished ...
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. ...
The caspase enzyme family is a central effector of apoptosis. The undynamic caspase will trigger apoptosis when it is activated ... Caspase-8 and p38MAPK in DATS-induced apoptosis of human CNE2 cells ... Caspase is an inactive enzyme zymogen under normal circumstances, but once activated it will trigger the caspase cascade, ... We tested the effects of DATS on p-p38 and caspase-8 activity as a function of dose by Western blotting. As shown in Figure 7, ...
  • It is caused by mutations in the CASP8 gene that encodes the protein caspase-8. (wikipedia.org)
  • Caspase-8 is a caspase protein, encoded by the CASP8 gene. (wikipedia.org)
  • The CASP8 gene encodes a member of the cysteine - aspartic acid protease ( caspase ) family. (wikipedia.org)
  • Mice with a conditional deletion of caspase-8 in the intestinal epithelium (Casp8(ΔIEC)) spontaneously developed inflammatory lesions in the terminal ileum and were highly susceptible to colitis. (nih.gov)
  • Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. (abcam.com)
  • Auf www.antikoerper-online.de finden Sie aktuell 434 Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antikörper von 38 unterschiedlichen Herstellern. (antikoerper-online.de)
  • Caspase 8 (Casp8) is essential for death-receptor-mediated apoptosis activity and therefore its modulation might be critical for the pathogenesis of NASH. (wur.nl)
  • On www.antibodies-online.com are 406 Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies from 37 different suppliers available. (antibodies-online.com)
  • Plays a role in the inhibition of apoptosis by interacting with the pro-domain of pro-caspase-8/CASP8 and thus preventing its activation. (uniprot.org)
  • Caspase-8 (Casp8)-mediated signaling triggers extrinsic apoptosis while suppressing receptor-interacting protein kinase (RIPK) 3-dependent necroptosis. (jimmunol.org)
  • The minor allele of two caspase 8 polymorphisms, namely CASP8 -652 6N InsDel (rs3834129) and CASP8 Asp302His (rs1045485), were repeatedly associated with reduced breast cancer susceptibility. (biomedcentral.com)
  • We observed an allele-dose dependent association between CASP8 -652 6N InsDel and caspase 8 mRNA expression in breast cancer tissue, with homozygous deletion carriers showing lowest relative caspase 8 expression ( p = 0.0131). (biomedcentral.com)
  • The non-coding CASP8 -652 6N InsDel polymorphism, a functional 6-bp deletion located in the promoter region of the CASP8 gene, has been associated with reduced CASP8 mRNA expression and concomitantly impaired caspase-8 activity and reduced "activation induced cell death" (AICD) in stimulated T-lymphocytes [ 3 ]. (biomedcentral.com)
  • The second polymorphism, CASP8 Asp302His, is located in the coding region of caspase 8 and results in aspartic acid to histidine substitution (Fig. 1a ). (biomedcentral.com)
  • Loss of caspase-8 in macrophages dictates the response to TLR activation, as injection of TLR ligands upregulates expression of costimulatory CD86 on the Ly6C high CD11b + F4/80 + splenic cells, and oral antibiotic treatment to remove microbiota prevents splenomegaly and lymphadenopathy in Cre LysM Casp8 fl/fl mice. (biomedcentral.com)
  • Here, we demonstrate that cleavage of the intersubunit linker of c-FLIP(L) by procaspase-8 potentiates the activation process by enhancing heterodimerization between the two proteins and vastly improving the proteolytic activity of unprocessed caspase-(C)8. (rcsb.org)
  • Cleavage of pNA peptides by caspases generates pNA that is monitored colorimetrically at ~405 nm. (anaspec.com)
  • The caspases constitute a family of aspartate-specific cysteine proteases that mediate a sequence of cleavage events. (thermofisher.com)
  • Recruitment of the inactive proenzyme to oligomerized receptors leads to caspase activation and autoproteolytic cleavage. (thermofisher.com)
  • Subsequent dimerization leads to caspase-8 activation via autocatalytic cleavage, which leads to the formation of a 12-kDa prodomain and a 43-kDa intermediate fragment that is further cleaved to produce 26-kDa and 18-kDa active enzymes. (thermofisher.com)
  • In these cells, following cleavage by caspase 8, the C-terminal fragment of Bid translocates to mitochondria and triggers the release of apoptogenic factors, thereby inducing cell death. (nih.gov)
  • When phosphorylated, Bid was insensitive to caspase 8 cleavage in vitro. (nih.gov)
  • Apoptosis in response to the ligand CD95L (also known as Fas ligand) is initiated by caspase-8, which is activated by dimerization and self-cleavage at death-inducing signaling complexes (DISCs). (sciencemag.org)
  • Previous work indicated that the degree of substrate cleavage by caspase-8 determines whether a cell dies or survives in response to a death stimulus. (sciencemag.org)
  • We derived and experimentally validated a minimal model in which cleavage of caspase-8 in the enzymatic domain occurs in an interdimeric manner through interaction between DISCs, whereas prodomain cleavage sites are cleaved in an intradimeric manner within DISCs. (sciencemag.org)
  • The activation of caspase-8 by combined intra- and interdimeric cleavage ensures weak signaling at low concentrations of CD95L and strongly accelerated activation at higher ligand concentrations, thereby contributing to precise control of apoptosis. (sciencemag.org)
  • Activation of caspases during apoptosis results in the cleavage of critical cellular substrates so precipitating the dramatic morphological changes of apoptosis. (biovendor.com)
  • Apart from being activated by CD95 cleavage of caspase-8 by granzymeB during T-lymphocyte induced apoptosis has been shown. (biovendor.com)
  • In apoptotic cells, caspase-8 undergoes two sequential autoproteolytic cleavage reactions that separate the large and small catalytic subunits as well as deleting a small linker peptide sequence (amino acids 374-384) and the prodomain (amino acids 1-216), releasing the mature tetramer form into the cytoplasm ( 10 ). (aacrjournals.org)
  • Recruitment of procaspase-8 into the death signaling complex leads to its dimerization, autoproteolytic cleavage and formation of a highly active heterotetramer. (haematologica.org)
  • The retention of Spi-2 proteins' caspase-8 specificity during chordopoxvirus evolution, despite this function being readily lost through cleavage site mutagenesis, suggests that caspase-8 inhibition is crucial for poxviral pathogenesis and spread. (portlandpress.com)
  • Further downstream, caspase-8 triggers the proteolytic activation of other caspases and cleavage of cellular substrates. (rupress.org)
  • The radiation induced cleavage pattern of procaspase-8 into mature caspase-8 resembled that following CD95 crosslinking and resulted in cleavage of the proapoptotic substrate BID. (diva-portal.org)
  • A high local concentration of caspase 8 zymogens is thought to facilitate self-processing and cleavage to the active enzyme ( 34 ). (asm.org)
  • Activated caspase 8 then initiates apoptosis by cleavage of the downstream effector caspases 3, 6, and 7 ( 10 ). (asm.org)
  • During apoptosis, pro-caspases 1 - 3 and 6 - 10 are processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. (vwr.com)
  • We show that A20-mediated ubiquitination inhibits caspase-8 cleavage and TRAIL-induced apoptosis in glioblastoma through 2 signaling complexes. (aacrjournals.org)
  • In the death-inducing signaling complex, the C-terminal zinc finger (Znf) domain of the A20 ubiquitin ligase mediates receptor-interacting protein 1 polyubiquitination through lysine-63-linked polyubiquitin chains, which bind to the caspase-8 protease domain and inhibit caspase-8 dimerization, cleavage, and the initiation of TRAIL-induced apoptosis in glioblastoma-derived cell lines and tumor-initiating cells. (aacrjournals.org)
  • The dimerization and cleavage of caspase-8 in the DISC are the critical upstream events in TNF family ligand-induced apoptosis ( 9 - 12 ), and ubiquitination of proteins in the DISC regulates these biochemical processes ( 13 ). (aacrjournals.org)
  • TRAF2 and RIP1 then detach from TNFR1 and recruit FADD and caspase-8 for the assembly of the cytoplasmic complex II ( 19 ), where the deubiquitinating cylindromatosis removes the polyubiquitin chains from RIP1 to promote caspase-8 cleavage for TNF-α-induced apoptosis ( 20 ). (aacrjournals.org)
  • Cullin 3 (CUL3), an E3 ligase, adds K48- and K63-linked polyubiquitin chains to caspase-8 and facilitates its dimerization and cleavage in the DISC, where A20 deubiquitinating enzyme removes the polyubiquitin chains from caspase-8 ( 23 ). (aacrjournals.org)
  • Caspase-8-mediated cleavage inhibits IRF-3 protein by facilitating its proteasome-mediated degradation. (bio-protocol.org)
  • Our studies indicate that IRF3 can be proteolytically processed by caspase-8-dependent cleavage (Sears et al . (bio-protocol.org)
  • 2011). The direct involvement of caspase-8 was confirmed by in vitro cleavage assay using recombinant proteins and in vivo by virus activated caspase-8. (bio-protocol.org)
  • The proteolytic cleavage of IRF3 can be inhibited by chemical inhibition or genetic ablation of caspase-8. (bio-protocol.org)
  • Caspases can be detected via immunoprecipitation, immuno-blotting techniques using caspase specific antibodies, or by employing fluorogenic substrates which become fluorescent upon cleavage by the caspase. (celltechnology.com)
  • Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. (antibody-antibodies.com)
  • During apoptosis procaspase-8 is processed at aspartate residues by selfproteolysis and/or cleavage by another caspase. (antibody-antibodies.com)
  • Oxidant-induced neuronal apoptosis has been shown to involve potassium and zinc dysregulation, energetic dysfunction, activation of stress-related kinases, and caspase cleavage. (jneurosci.org)
  • Moreover, we found that activation of p38 is required for caspase 3 and 9 cleavage, suggesting that potassium currents enhancement is required for caspase activation. (jneurosci.org)
  • Based on these findings, we conclude that oxidation of sulfhydryl groups on intracellular targets results in intracellular zinc release, p38 phosphorylation, enhancement of potassium currents, caspase cleavage, energetic dysfunction, and translationally independent apoptotic cell death. (jneurosci.org)
  • Activation involves dimerization and often oligomerisation of pro-caspases, followed by cleavage into a small subunit and large subunit. (wikipedia.org)
  • Caspase-8 is a 51 kb gene with 13 exons encoding for a 496 amino acid protein that maps to 2q33.1. (wikipedia.org)
  • The mutations lead to functional caspase-8 deficiency by destabilizing the caspase-8 protein and inactivating its enzymatic capacity. (wikipedia.org)
  • For the death pathway, the caspase-8 zymogen is cleaved into subunits that assemble to form the mature, highly active caspase heterotetramer whereas for the activation pathway, the zymogen appears to remain intact perhaps to limit its proteolytic function but enhance its capability as an adapter protein. (wikipedia.org)
  • Background: Activation of Fas (CD95) by its ligand (FasL) rapidly induces cell death through recruitment and activation of caspase-8 via the adaptor protein Fas-associated death domain protein (FADD). (mendeley.com)
  • Results: Under conditions in which proliferation of CD3-activated human T lymphocytes is increased by recombinant FasL, there was activation of the transcription factors NF-κB and AP-1 and recruitment of the caspase-8 inhibitor and FADD-interacting protein FLIP (FLICE-like inhibitory protein). (mendeley.com)
  • Instead, the loss of Casp-8 caused hyperactivation of p38 MAPK downstream of receptor-interacting serine/threonine protein kinase 3 (RIPK3) and destabilization of vascular endothelial cadherin (VE-cadherin) at EC junctions. (jci.org)
  • Each Caspase-8 Peptide and Caspase-8 Protein is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • The CED-4-homologous protein FLASH is involved in Fas-mediated activation of caspase-8 during apoptosis [see comments] [published erratum appears in Nature 1999 Jul 1;400(6739):89]. (jax.org)
  • Zusätzlich bieten wir Ihnen Caspase 8 Kits (79) und Caspase 8 Proteine (26) und viele weitere Produktgruppen zu diesem Protein an. (antikoerper-online.de)
  • The N-terminal FADD-like death effector domain of Caspase 8 suggests that it may interact with Fas-interacting protein FADD. (thermofisher.com)
  • Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the pro-form of the Caspase 8 protein. (thermofisher.com)
  • Additionally we are shipping Caspase 8 Kits (72) and Caspase 8 Proteins (26) and many more products for this protein. (antibodies-online.com)
  • In this study, we investigated the function of p38 mitogen-activated protein kinase (MAPK) and caspase-8 in DATS-induced apoptosis of human CNE2 cells using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], flow cytometry assay, and Western blotting. (scielo.br)
  • The CARD8 gene encodes different CARD8 isoforms that contain the caspase-associated recruitment domain (CARD) in their carboxy-termini that acts as a protein-protein interaction interface. (atlasgeneticsoncology.org)
  • The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system. (scbt.com)
  • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for caspase-3 Antibody (E-8) for WB and IHC(P) applications. (scbt.com)
  • caspase-3 Antibody (E-8) is a high quality monoclonal caspase-3 antibody (also designated CASP3 antibody or CPP32 antibody) suitable for the detection of the caspase-3 protein of human origin. (scbt.com)
  • I found that caspase-8 and receptor-interacting protein kinase-3 (RIPK3), RIPK1 and FADD are required for Yersinia-induced cell death. (upenn.edu)
  • This enhanced caspase-8 oligomerization and activation are promoted through its interaction with the ubiquitin-binding protein SQSTM1/p62 and the microtubule-associated protein light chain 3 (LC3), which are enriched at intracellular membranes in response to proteotoxic stress. (asm.org)
  • Our results unveiled a previously unknown mechanism through which disruption of protein homeostasis induces caspase-8 oligomerization, activation, and apoptosis. (asm.org)
  • Upon activation of Fas by its ligand, the DD undergoes homotypic interaction with a DD in the adaptor protein FADD, which then recruits the initiator caspase 8 via their mutual N-terminal death effector domains (DED) ( 3 ). (asm.org)
  • This event leads to the recruitment and activation of an adapter protein, FADD (Fas-associated death domain-containing protein), and to the subsequent activation of caspases, a family of cysteine proteases that exist as inactive zymogens in normal cells ( 5 , 34 ). (asm.org)
  • Protein level changes of procaspase 8, procaspase 9 and cleaved caspase 9 were determined by Western blot. (arvojournals.org)
  • A gene on chromosome 2q33-q34 that encodes a protein belonging to the cysteine-aspartic acid protease (caspase) family which, once activated by proteolytic processing, plays a central role in the execution phase of cell apoptosis, as well as various stages of embryological development. (thefreedictionary.com)
  • Chen YC, Shen SC, Lee WR, Lin HY, Ko CH, Shih CM, Yang LL (2002) Wogonin and fisetin induction of apoptosis through activation of caspase 3 cascade and alternative expression of p21 protein in hepatocellular carcinoma cells SK-HEP-1. (springer.com)
  • In this study, we show that sphingosine induces death receptor-independent caspase-8 activation and apoptotic cell death via p38 mitogen-activated protein kinase (MAPK) activation and that suppression of the MAPK/extracellular signal-regulated kinase (ERK) kinase/ERK pathway by protein phosphatase 2A (PP2A) is required for p38 MAPK activation. (aacrjournals.org)
  • Caspase (CASP)8 is an autoactivating aspartate-specific cysteine protease that initiates extrinsic apoptosis and prevents receptor interacting protein (RIP) kinase (RIPK)1-RIPK3-driven necroptosis. (escholarship.org)
  • Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. (antibody-antibodies.com)
  • Fas-AssociatedDeathDomain (FADD) and receptor interacting protein 1 (RIP1) are death domain containing molecules that interact with the C-terminal portion of IPS-1 and induce NF-kB through interaction and activation of initiator caspases (caspase-8 and -10). (reactome.org)
  • Among the extrinsic pathway, caspase-8 cleaves the Bcl-2 related protein Bid, which in turn induces cytochrome c release from mitochondria and caspase-3 activation, likewise resulting in apoptosis [ 1 , 2 ]. (biomedcentral.com)
  • However, it remains largely unknown how apparently disparate events implicated in apoptosis, such as oxidative stress, ionic dysregulation, and activation of mitogen-activated protein kinases (MAPK) and caspases, signal among one another to initiate and propagate cell death. (jneurosci.org)
  • They are named caspases due to their specific cysteine protease activity - a cysteine in its active site nucleophilically attacks and cleaves a target protein only after an aspartic acid residue. (wikipedia.org)
  • This protein aggregation facilitates recruitment of the cysteine-aspartic acid enzyme pro-caspase-8, which becomes active upon dimerization. (biomedcentral.com)
  • Active caspase-8 initiates the degradative phase of apoptosis through activation of caspases-3/7 or blocks necroptosis via suppression of receptor-interacting serine/threonine protein kinase (RIPK)1-RIPK3 signaling, depending upon the availability of cellular FADD-like interleukin (IL)-1β-converting enzyme-inhibitory protein (cFLIP) [ 1 , 2 ]. (biomedcentral.com)
  • A caspase is a type of protease that when activated cleaves protein substrates. (biocompare.com)
  • Biochemically, caspase-8 was found to enter the complex of the inhibitor of NF-κB kinase (IKK) with the upstream Bcl10-MALT1 (mucosa-associated lymphatic tissue) adapter complex which were crucial for the induction of nuclear translocation of NF-κB. (wikipedia.org)
  • The UL36 gene product inhibits a more proximal event in Fas-mediated apoptosis by complexing with pro-caspase-8 in a way that suppresses its proteolytic activation, prompting its designation as viral inhibitor of caspase-8-induced apoptosis (vICA). (pnas.org)
  • The caspase-8 inhibitor FLIP promotes act. (mendeley.com)
  • In the present study, we used SB203580, a p38MAPK inhibitor, and Z-LETD-FMK, a caspase-8 inhibitor, to determine the relation of p38MAPK and caspase-8 in the apoptosis process induced by DATS. (scielo.br)
  • Fluorescein (FITC)-conjugated IETD-FMK, a specific inhibitor of caspase-8, is utilized in this assay for detection. (thermofisher.com)
  • When detecting caspase-8, we utilize a specific FITC-conjugated caspase-8 inhibitor, IETD-FMK. (nexcelom.com)
  • Besides a general caspase inhibitor, caspase-2 and 8- specific inhibitors largely abrogated HOXA5-induced apoptosis whereas caspase-1, 3, 6 and 9-specific inhibitors had no significant effects. (aacrjournals.org)
  • Our inhibitor studies further implicated CaMKII g in the activation of extracellular signal-regulated kinases 1 and 2 (ERK 1/2) culminating in caspase-8/caspase-3 mediated apoptosis of HKM cells. (fluoridealert.org)
  • A potent, cell-permeable, and irreversible inhibitor of caspase-8 and granzyme B. Effectively inhibits influenza virus-induced apoptosis in HeLa cells. (alfa.com)
  • This in vitro assay employs the fluorescent inhibitor probe FAM-LETD-FMK to label active caspase-8 enzyme in living cells. (immunochemistry.com)
  • In the present study, we first treated a number of breast cancer cell lines with the proteasome inhibitor MG132 and observed that caspase-8 activation plays an important role in MG132-induced apoptosis. (asm.org)
  • The caspase 8 inhibitor c-FLIP L can act in vitro as a molecular switch between cell death and growth signals transmitted by the death receptor Fas (CD95). (asm.org)
  • Moreover, treatment of HeLa cells with fisetin induced a sustained activation of the phosphorylation of ERK1/2, and inhibition of ERK1/2 by PD98059 (MEK1/2 inhibitor) or transfection with the mutant ERK1/2 expression vector significantly abolished the fisetin-induced apoptosis through the activation of caspase-8/-3 pathway. (springer.com)
  • Interferon-gamma-induced sensitization of colon carcinomas to ZD9331 targets caspases, downstream of Fas, independent of mitochondrial signaling and the inhibitor of apoptosis survivin. (semanticscholar.org)
  • Once inside the cell, the inhibitor binds covalently to the active caspase (10). (celltechnology.com)
  • Although ScA initiates apoptosis via both the intrinsic and extrinsic pathways, the more sensitive pathway involves activation of caspase 8, which requires concentrations in the low nanomolar range and below, to initiate alterations in the cell membrane and apoptosis. (pnas.org)
  • The selective activation of the caspase 8 pathway by a small molecule is promising, because few currently used anticancer agents have this property. (pnas.org)
  • Caspase-8 (Casp-8), a key protease in the extrinsic cell death-signaling pathway, regulates cell death via both apoptosis and necroptosis. (jci.org)
  • In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells. (hindawi.com)
  • The extrinsic pathway is activated by the binding of ligands (including TNFα, FasL, and TRAIL) to cell-surface receptors and the conversion of procaspase-8 to the active caspase-8 protease. (nexcelom.com)
  • Because Caspase-8 activation is such an early step in the extrinsic apoptosis pathway, measurement of Caspase-8 is an interesting alternative to measurement of apoptosis via Annexin V-FITC / PI, which involves binding to phosphatidylserine (PS) on the surface of apoptotic cells. (nexcelom.com)
  • Furthermore, we demonstrate that caspase-1-independent apoptosis requires the activation of caspase-9 and of the intrinsic pathway in a typical type II cell manner. (archives-ouvertes.fr)
  • Finally, we identify the AIM2/ASC-dependent caspase-1-independent pathway as an innate immune mechanism able to restrict bacterial replication in vitro and control IFN-γ levels in vivo in Casp1(KO) mice. (archives-ouvertes.fr)
  • In this work, we identified that the caspase-1/11-independent pathway is composed of Naip5/NLRC4/ASC/Caspase-8 and it is essential for the control of flagellated Legionella spp. (usp.br)
  • Caspase-3, caspase-7, and caspase-8 are important caspases in the apoptosis pathway and play an important role in the development and progression of cancer. (aacrjournals.org)
  • The most studied are the steroid metabolism pathway genes ( 2 - 4 ), cell-cycle control pathway genes ( 5 - 7 ), and DNA repair pathway genes ( 8 , 9 ). (aacrjournals.org)
  • EG also activated the death receptor-dependent pathway of apoptosis by enhancing the expression of caspases-8, -9, and -3 and the Bcl-2 interacting domain (Bid). (mdpi.com)
  • Caspase-8 subsequently activates caspase-3, thereby initiating the proteolytic pathway, and ultimately resulting in the apoptotic disassembly of the cell. (haematologica.org)
  • These results suggest that chemotherapeutic drug-induced caspase activation is entirely controlled by a receptor-independent mitochondrial pathway, whereas CD95-induced apoptosis can be regulated by a separate pathway not requiring Apaf-1 function. (rupress.org)
  • We conclude that radiation activates caspase-8 via an Lck-controlled pathway independently of CD95 ligand expression, This is a novel signaling event required for radiation induced apoptosis in T lymphoma cells. (diva-portal.org)
  • In clinical trials, however, investigators have proven that cancers are resistant to TRAIL pathway-targeted therapies ( 6 - 8 ), thus suggesting that cancers escape from TRAIL-mediated immunosurveillance and are resistant to TRAIL-targeted therapies. (aacrjournals.org)
  • A close relationship exists between the ERK pathway and the p38 MAPK or JNK pathway in a variety of eukaryotic cells ( 8 - 11 ). (aacrjournals.org)
  • Furthermore, up-regulation of caspase-3 and caspase-8 activities substantiated the induction of apoptosis through caspase-dependent pathway. (nottingham.ac.uk)
  • Among the death receptor signaling pathway, the initiator Caspase 8, a 55 kDa cysteine protease, plays an important role in intrinsic and extrinsic apoptosis induction. (biomedcentral.com)
  • In terms of the intrinsic apoptosis pathway, caspase-8 activates the death inducing signaling complex (DISC), which in turn induces downstream effector caspase-3, finally resulting in apoptosis [ 1 , 2 ]. (biomedcentral.com)
  • These studies tested if prostaglandin F 2α (PGF 2α ) or FAS regulated luteal regression, utilize a caspase -3 dependent pathway to execute luteal cell apoptosis, and if the two receptors work via independent or potentially shared intracellular signaling components/pathways to activate caspase -3. (biomedcentral.com)
  • Cytotoxicity of ICD-85 NPs on Human Cervical Carcinoma HeLa Cells through Caspase-8 Mediated Pathway', Iranian Journal of Pharmaceutical Research , 12(1), pp. 155-163. (ac.ir)
  • The clinical phenotype of CEDS patients represented a paradox because caspase-8 was considered to be chiefly a pro-apoptotic protease, that was mainly involved in signal transduction. (wikipedia.org)
  • Caspases exist as inactive proenzymes composed of a prodomain , a large protease subunit , and a small protease subunit. (wikipedia.org)
  • The clinical phenotype of CEDS patients represented a paradox since caspase-8 was considered to be chiefly a proapoptotic protease , that was mainly involved in signal transduction from Tumor necrosis factor receptor family death receptors such as Fas. (wikipedia.org)
  • The crystal structures of the protease-like domain of c-FLIP(L) alone and in complex with zymogen C8 identify the unique determinants that favor heterodimerization over procaspase-8 homodimerization, and induce the latent active site of zymogen C8 into a productive conformation. (rcsb.org)
  • Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. (abcam.com)
  • Caspase-8 is a cysteine protease best known for mediating signals downstream of death receptors leading to apoptosis. (sciencemag.org)
  • Knockout of caspase-8 , a cysteine protease that participates in the signaling for cell death by receptors of the TNF/nerve growth factor family, is lethal to mice in utero. (jimmunol.org)
  • The caspase cysteine protease family is known mainly for the participation of some of its members in programmed cell death in eukaryotes ( 2 ). (jimmunol.org)
  • Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. (thermofisher.com)
  • Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. (scbt.com)
  • Caspase-8 is a cysteine protease, which cleaves downstream substrates such as effector caspases, to initiate the apoptotic cascade and transmit apoptotic signals downstream of death receptors ( 16 ). (aacrjournals.org)
  • Caspase-8 is a member of the aspartate-specific cysteine protease family that is typically synthetized as an inactive zymogen and activated upon an appropriate stimulus. (haematologica.org)
  • A synthetic peptide that irreversibly inhibits FLICE and related protease/caspase activity and blocks apoptosis. (creative-enzymes.com)
  • In all cases, apoptosis is mediated by caspases, although it is unclear how these diverse apoptotic stimuli cause protease activation. (rupress.org)
  • Caspases ( c ysteine- asp artic prote ases , c ysteine asp art ases or c ysteine-dependent asp artate-directed prote ases ) are a family of protease enzymes playing essential roles in programmed cell death (including apoptosis , pyroptosis and necroptosis ) and inflammation . (wikipedia.org)
  • Caspase 7 (Apoptotic Protease Mch 3 or ICE Like Apoptotic Protease 3 or CMH 1 or CASP7 or EC 3.4.22.60) pipeline Target constitutes close to 5 molecules. (researchandmarkets.com)
  • Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. (wikipedia.org)
  • Homology between the sense portion of the siRNA sequence and the mRNA enables the nuclease enzyme to bind and cleave the caspase transcript into small pieces, which are degraded by the cell's machinery. (abcam.com)
  • 1998). Enzymatic activity of two caspases related to interleukin-1beta-converting enzyme. (anaspec.com)
  • 2000). Caspase 8: an efficient method for large-scale autoactivation of recombinant procaspase 8 by matrix adsorption and characterization of the active enzyme. (anaspec.com)
  • However, application of the conditional knockout approach to investigate the cause of death of caspase-8 knockout embryos revealed that this enzyme also serves cellular functions that are nonapoptotic. (jimmunol.org)
  • Caspase is an inactive enzyme zymogen under normal circumstances, but once activated it will trigger the caspase cascade, eventually leading to apoptosis. (scielo.br)
  • As an initiator caspase, this enzyme initiates a caspase signaling cascade that results in apoptosis. (thermofisher.com)
  • The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. (scbt.com)
  • The remaining green fluorescent signal is a direct measure of the active caspase-8 enzyme activity present in the cell at the time the reagent was added. (immunochemistry.com)
  • The recombinant human caspases 1 - 3 and 6 - 10 are members of the ICE (interleukin-1 β converting enzyme) family of cysteine proteases. (vwr.com)
  • The matured (processed) form of these caspases consists of large and small subunits, which associate to form a heterotetramer active enzyme. (vwr.com)
  • One unit of the recombinant caspase-8 is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT. (antibody-antibodies.com)
  • The lyophilized caspase-8 is stable for 1 year at -70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at -70°C. Avoid multiple freeze/thaw cycles as activity might decrease. (antibody-antibodies.com)
  • Active caspase-8 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. (antibody-antibodies.com)
  • Structure of caspase-1 (CASP1), originally called interleukin-1 beta-converting enzyme (ICE), the first human caspase to be identified. (wikipedia.org)
  • The active enzyme often exists as a heterotetramer in the biological environment, where a pro-caspase dimer is cleaved together to form a heterotetramer. (wikipedia.org)
  • Although caspase-8 is a well-established initiator of apoptosis and suppressor of necroptosis, recent evidence suggests that this enzyme maintains functions beyond its role in cell death. (biomedcentral.com)
  • Ovaries from each group were collected 8 h later for assessment of active caspase -3 enzyme and apoptosis (measured by the TUNEL assay) in the CL. (biomedcentral.com)
  • The following product was used in this experiment: Caspase 8 Polyclonal Antibody from Thermo Fisher Scientific, catalog # 13423-1-AP. (thermofisher.com)
  • This antibody can recognize the pro- and cleaved-caspase 8. (thermofisher.com)
  • Caspase-8 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus human caspase-8 isoform E.The immunogen is located within the last 50 amino acids of Caspase-8. (genetex.com)
  • WB analysis of HT-29 cell lysate in (A) the presence or (B) the absence of blocking peptide using GTX31285 Caspase 8 antibody. (genetex.com)
  • This reagent is now offered in a bundle with caspase-3 Antibody (E-8) ( see ordering information below ). (scbt.com)
  • caspase-3 Antibody (E-8) is available as both the non-conjugated anti-caspase-3 antibody form, as well as multiple conjugated forms of anti-caspase-3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor ® conjugates. (scbt.com)
  • Caspase-8 Antibody antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of Caspase-8 Antibody. (antibody-antibodies.com)
  • 100 µg (0.5 mg/ml) affinity purified rabbit anti-caspase-8 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. (antibody-antibodies.com)
  • The affinity purified antibody mainly detects the 26 kDa fully cleaved fragment and the 35-43 kDa intermediate of caspase-8 in apoptotic samples. (antibody-antibodies.com)
  • Systemic treatment of zebrafish or local treatment of the chick chorioallantoic membrane with ScA resulted in dose-dependent inhibition of angiogenesis, whereas topical treatment blocked tumor growth among caspase-8-expressing tumors. (pnas.org)
  • and mutation of the caspase 8 processing site on the substrate should convert a pro-survival response to necrotic death without the need for caspase 8 inhibition. (nih.gov)
  • We further show that AIM2 engagement leads to ASC-dependent, caspase-1-independent activation of caspase-8 and caspase-9 and that caspase-1-independent death is reverted upon caspase-8 inhibition. (archives-ouvertes.fr)
  • IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases. (alfa.com)
  • All potently blocked caspases-1, -4, -5 and -8 activity but exhibited negligible inhibition of caspases-2, -3 and -6. (portlandpress.com)
  • In contrast, inhibition of ATP production did not affect caspase activation after triggering of CD95. (rupress.org)
  • We show here that inhibition of proteasomal degradation results in an increased oligomerization and activation of caspase-8 on the cytosolic side of intracellular membranes. (asm.org)
  • We went on to study the molecular mechanism and significance of caspase-8 activation in response to proteasome inhibition. (asm.org)
  • The above results indicate that MUC1 gene silencing induces growth inhibition in SMMC‑7721 cells through Bax‑mediated mitochondrial and caspase-8-mediated death receptor apoptotic pathways. (spandidos-publications.com)
  • Inhibition of p38 MAPK led to the marked suppression of death receptor-independent caspase-8 activation and subsequent cell death induced by sphingosine. (aacrjournals.org)
  • Indeed, an early robust increase in TEA-sensitive potassium channel currents induced by DTDP is attenuated by p38 inhibition but not by caspase inhibition. (jneurosci.org)
  • Note that in addition to apoptosis, Caspase-8 is also required for the inhibition of another form of programmed cell death called Necroptosis . (wikipedia.org)
  • We aimed to clarify whether activation of caspases and Fas signalling are crucial for the onset of apoptosis after specific inhibition of TS and whether p53 plays a role in activation of these downstream processes. (elsevier.com)
  • In conclusion, these results indicate that apoptosis induced after specific inhibition of TS is mediated via the caspases, but without clear involvement of Fas signalling. (elsevier.com)
  • Caspase-8, which is also known as FLICE, MACHalpha1, and Mch5, cleaves its substrates at the C-terminal aspartic acid residue of the motif Asp-X-X-Asp. (thermofisher.com)
  • Caspase-8 (also know as FLICE, MASH, Mch5) is a member of the caspase-family of cysteine proteases. (antibody-antibodies.com)
  • By interfering with caspase-8 activation, vICA functions in a manner similar to the viral and cellular FLIPs ( 8 ), but lack of any sequence homology suggests that this CMV death suppressor may represent a new class of viral and potentially cellular antiapoptotic proteins. (pnas.org)
  • We offer Caspase-8 Peptides and Caspase-8 Proteins for use in common research applications: Blocking/Neutralizing, Control, Functional, SDS-Page. (novusbio.com)
  • Our Caspase-8 Peptides and Caspase-8 Proteins can be used in a variety of model species: Human, Mouse. (novusbio.com)
  • Choose from our Caspase-8 Peptides and Proteins. (novusbio.com)
  • Within this family of proteins is caspase-8, which is unusual as it can promote both cell death, and inflammatory gene expression induced by Toll-like Receptors. (upenn.edu)
  • In principle, it could also facilitate the interactions of caspase-8 promigratory proteins containing phosphotyrosine binding domains. (aacrjournals.org)
  • Spi-2 proteins like CrmA potently inhibit caspases-1, -4 and -5, which produce proinflammatory cytokines, and caspase-8, which facilitates cytotoxic lymphocyte-mediated target cell death. (portlandpress.com)
  • For example, active caspase-8 is also known to activate Bid, a proapoptotic member of the Bcl-2 family of proteins that can trigger a loss in mitochondrial transmembrane potential and cause an efflux of cytochrome c into the cytoplasm ( 26 ). (asm.org)
  • Moreover, we used recombinant proteins in vitro to conclude that IRF3 is a substrate of caspase-8 (Sears et al . (bio-protocol.org)
  • Caspase_8, mouse recombinant recombinant proteins Most stable storage is at - 81 C or lower but some lyophilised proteins can be stored at +4C. (antibody-antibodies.com)
  • Caspase_8, mouse recombinant mus musculus murine Caspase_8, mouse recombinant detects proteins from variouse species most likely human. (antibody-antibodies.com)
  • Active caspase-8 has been shown to activate caspase-3 leading to degradation of a variety of cellular target proteins during apoptosis. (antibody-antibodies.com)
  • Tumour growth can occur by a combination of factors, including a mutation in a cell cycle gene which removes the restraints on cell growth, combined with mutations in apoptopic proteins such as Caspases that would respond by inducing cell death in abnormally growing cells. (wikipedia.org)
  • GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Caspase_8, human recombinant. (antibody-antibodies.com)
  • pNA (4-nitroaniline)-derived caspase substrates are widely used for the colorimetric detection of various caspase activities. (anaspec.com)
  • Substrates of caspase-8 include caspases-3 and -7, as well as the pro-apoptotic Bcl-2 family member Bid. (thermofisher.com)
  • In that scenario, pro-caspase-8 could potentially cleave key substrates for motility-remaining to be identified-or could function noncatalytically as an interaction partner for a motility factor. (aacrjournals.org)
  • Caspases lead to the proteolysis of a number of cellular substrates, a process which finally results in the apoptotic collapse of the cell. (rupress.org)
  • The active caspase-8 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. (antibody-antibodies.com)
  • 1997). Substrate specificities of caspase family proteases. (anaspec.com)
  • The caspases represent a family of sulphydryl proteases which play important regulatory roles within the cell. (ihcworld.com)
  • Caspase-8 belongs to a family of evolutionally conserved cysteine proteases that play a key role in regulating programmed cell death or apoptosis. (nexcelom.com)
  • Caspases are proteases that are best characterized for their abilities to regulate apoptotic or pyroptotic cell death programs, both of which are critical for the proper operation of the mammalian immune system. (upenn.edu)
  • Proteases of the caspase family are the critical executioners of apoptosis. (semanticscholar.org)
  • As is common with other proteases, caspases are synthesized as precursors that undergo proteolytic maturation, either autocatalytically or in a cascade by enzymes with similar specificity (5). (celltechnology.com)
  • Caspase family of cysteine proteases has been shown to play a key role in apoptosis. (antibody-antibodies.com)
  • Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby committing the cell to undergo apoptosis. (thermofisher.com)
  • The results indicated that DATS activates p38MAPK and caspase-8, but both inhibitors have an effect on P38MAPK and caspase-8 activity. (scielo.br)
  • Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. (scbt.com)
  • Caspase-3 also cleaves and activates caspase-6, -7 and -9. (scbt.com)
  • Cytochrome c then complexes with Apaf-1 to activate procaspase-9, which in turn targets and activates caspase-3 ( 22 , 42 ). (asm.org)
  • Caspase-8, also called FLICE, has an N-terminal domain with sequence homology to the death effector domain of FADD that allows association of caspase-8 with the TNF/Fas family of receptors. (ihcworld.com)
  • Caspase-8 is activated by association with the Fas/FADD death-inducing signaling complex to release two active subunits, p18 and p10, into the cytosol where they activate other caspases amplifying the apoptotic signal. (ihcworld.com)
  • FADD, in addition, contains an NH 2 -terminal so-called death effector domain (DED), which binds to one of the DEDs of caspase-8. (rupress.org)
  • As such, v-FLIP can be recruited into the death-inducing signaling complex (DISC) of Fas, thereby competing with recruitment of caspase 8 to FADD. (asm.org)
  • We determined that the mechanism of influenza virus-induced apoptosis involved death signaling through FADD/caspase-8 activation, while other viruses such as vesicular stomatitis virus (VSV) and Sindbis virus (SNV) did not significantly provoke PKR-mediated apoptosis but did induce cytolysis of fibroblasts via activation of caspase-9. (asm.org)
  • The apical target of FADD is caspase-8/FLICE ( 30 ). (asm.org)
  • The anticancer activity of TRAIL is attributable to its ability to induce apoptosis through the binding of death receptors 4 and 5 (DR4, DR5) and the recruitment of intracellular apoptosis-initiating caspase-8 through Fas-associated death domain (FADD) for the assembly of a death-inducing signaling complex (DISC) ( 4 ). (aacrjournals.org)
  • In contrast to TNFR1, DR4 and DR5 recruit FADD and caspase-8 in the assembly of a plasma membrane-bound DISC, where caspase-8 becomes dimerized and cleaved, initiating apoptosis ( 21 , 22 ). (aacrjournals.org)
  • Cagnol S, Van Obberghen-Schilling E, Chambard JC (2006) Prolonged activation of ERK1, 2 induces FADD-independent caspase 8 activation and cell death. (springer.com)
  • Caspase-8 deficiency (CEDS) is a very rare genetic disorder of the immune system. (wikipedia.org)
  • This disease, called CEDS, stands for "Caspase eight deficiency state. (wikipedia.org)
  • However, deletion of caspase-8 in mice resulted in death in utero, which was associated with cardiac deformations, neural tube defects, and hemopoietic progenitor deficiency ( 13 , 14 ). (jimmunol.org)
  • The 3 known defects of lymphocyte apoptosis, Fas deficiency, FasL deficiency, and caspase 10 deficiency all have a similar phenotype: the early presentation of significant adenopathy, splenomegaly, and autoimmune disease. (aappublications.org)
  • Caspase-8 is now known to have an undefined but integral function in lymphocyte activation and the phenotype of patients with caspase-8 deficiency reflects this, with both lymphoid expansion and immunodeficiency. (aappublications.org)
  • Caspase deficiency has been identified as a cause of tumour development. (wikipedia.org)
  • yet, caspase-8-deficient macrophages are not predisposed to unchecked survival, as analysis of mixed bone marrow chimeric mice demonstrates that caspase-8 deficiency does not confer preferential expansion of myeloid populations. (biomedcentral.com)
  • 20% of patients CEDS: Caspase 8 deficiency state. (wikipedia.org)
  • Some reports mention an apoptosis inhibitory function of CARD8 involving its CARD-dependent binding to procaspase-9, whereas others did not find an association between CARD8 and caspase-9 and instead found either pro-apoptotic activity of CARD8 and associations with the inflammatory caspase-1 or the regulatory subunit of IkB kinase (NEMO) thereby suppressing NF-kB activation. (atlasgeneticsoncology.org)
  • The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. (scbt.com)
  • Stimulation of motility with epidermal growth factor induced the phosphorylation of caspase-8 on tyrosine-380 and the interaction of caspase-8 with the p85α subunit of phosphatidylinositol 3-kinase. (aacrjournals.org)
  • ref. 5 ), as well as through direct interactions of Rac, cdc42, and Rac-GEFs with the p85 regulatory subunit ( 6 , 7 ), which are involved in cdc42-mediated c-Jun-NH 2 -kinase activation ( 8 ). (aacrjournals.org)
  • In this report, we show that Y380-phosphorylated pro-caspase-8 interacts with the p85 subunit of PI3K, promoting cell adhesion and motility. (aacrjournals.org)
  • The large and small subunit associate with each other to form an active heterodimer caspase. (wikipedia.org)
  • however, the key substrate processed by caspase 8 to block necrosis has been elusive. (nih.gov)
  • We now identify CYLD as a substrate for caspase 8 that satisfies these criteria. (nih.gov)
  • Caspase enzymes specifically recognize a 4 amino acid sequence (on their substrate) which necessarily includes an aspartic acid residue. (celltechnology.com)
  • ScA Induces Apoptosis Selectively via Caspase 8. (pnas.org)
  • Overexpression of Caspase 8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. (thermofisher.com)
  • We conclude that fluoride -induced apoptosis is largely dependent on Ca 2+ induced superoxide generation leading to elevation in CaMKII g which in turn induces the phosphorylation of ERK 1/2 and downstream activation of extrinsic caspase cascade in HKM cells. (fluoridealert.org)
  • Kim W-H, Song H-O, Choi H-J, Bang H-I, Choi D-Y, Park H. Ethyl Gallate Induces Apoptosis of HL-60 Cells by Promoting the Expression of Caspases-8, -9, -3, Apoptosis-Inducing Factor and Endonuclease G. International Journal of Molecular Sciences . (mdpi.com)
  • We provide evidence that ionizing radiation induces a rapid activation of caspase-8 (FLICE) followed by apoptosis independently of CD95 ligand/receptor interaction. (diva-portal.org)
  • During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. (scbt.com)
  • During apoptosis in humans, initiator caspases integrate molecular signals into proteolytic activity ( 11 ) and subsequently activate the downstream effector caspases, thus transmitting and amplifying the apoptotic signal ( 12 ). (aacrjournals.org)
  • The central component of this process is a cascade of proteolytic enzymes called caspases. (celltechnology.com)
  • In apoptosis, caspases are responsible for proteolytic cleavages that lead to cell disassembly (effector caspases), and are involved in upstream regulatory events (initiator caspases). (celltechnology.com)
  • ICT's FLICA assay kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues. (immunochemistry.com)
  • The FAM FLICA Caspase-8 assay probe allows researchers to assess caspase-8 activation. (immunochemistry.com)
  • MitoCasp A simultaneous dual parameter Assay For: Mitochondrial Membrane Potential Detection & Caspase (poly, 3/7, 8, 9, 1) Activity. (celltechnology.com)
  • We recommend using 1 unit/assay for analyzing caspase activity. (antibody-antibodies.com)
  • For a complete caspase-8 assay protocol, please refer to BioVision's Caspase-8 Fluorometric or Colorimetric Assay Kits (Cat. (antibody-antibodies.com)
  • Your search returned 768 Apoptosis Caspase Assay Cell Viability and Proliferation across 19 suppliers. (biocompare.com)
  • The apoptotic effect of free ICD-85 and ICD-85 NPs on HeLa cells was assessed using caspase-8 colorimetric assay. (ac.ir)
  • Moreover, caspase-8 assay analysis demonstrated that the ICD-85 NPs induced a higher apoptotic rate in HeLa cells compared to free ICD-85. (ac.ir)
  • The purpose of this study was to use laser capture microdissection (LCM) to isolate RGC coupled with real-time PCR to test the hypothesis that gene expression of initiator caspases would be elevated during RGC death in experimental glaucoma. (arvojournals.org)
  • After human CNE2 cells were treated with 100 μM DATS and inhibitors (10 μM SB203580 and Z-LETD-FMK for p38MAPK and caspase-8, respectively), changes in cell viability and apoptosis and in p38MAPK and caspase-8 activity were detected. (scielo.br)
  • The methodology is based on carboxyfluorescein (FAM) labeled fluoromethyl ketone (FMK)-peptide inhibitors of caspases. (celltechnology.com)
  • In contrast, simultaneous exposure of Lovo and WiDr cells to AG337 and inhibitors of caspases 8, 9 and 3 caused a decrease in the number of apoptotic cells compared with AG337 exposure alone. (elsevier.com)
  • The translocation of PS to the surface of the cell membrane occurs downstream in the apoptotic process and identifies cells committed to death through either the extrinsic or intrinsic apoptosis pathways following activation of Caspase-3 2 . (nexcelom.com)
  • In this study, we report the establishment of conditional knockout of the caspase-8 gene using the Cre/ loxP recombination system, and its use for exploring these other functions. (jimmunol.org)
  • Mutations in the caspase-8 gene not only affect apoptosis but also affect host defense. (aappublications.org)
  • Since uncleaved caspase-8 functions together with its homolog, cFLIP, our findings implicate the activity of a caspase-8/cFLIP heterodimer in control of inflammatory cytokines during microbial infection, and provide new mechanistic insight into how caspase-8 regulates gene expression. (upenn.edu)
  • Of 35 selected single-nucleotide polymorphisms, four in the caspase-7 gene were in high linkage disequilibrium (rs11593766, rs3124740, rs11196445, and rs11196418) and associated with the risk for endometrial cancer. (aacrjournals.org)
  • No association was observed between polymorphisms of the caspase-8 gene and risk for endometrial cancer. (aacrjournals.org)
  • Further investigation using western blotting revealed that cytochrome c was released from the mitochondria into the cytoplasm, and caspase‑8 and caspase‑9 were activated in MUC1 gene‑silenced SMMC‑7721 cells. (spandidos-publications.com)
  • In addition, results from the co‑immunoprecipitation experiments demonstrated that the MUC1 cytoplasmic tail can bind directly to Bax or caspase‑8 and these interactions were reduced upon MUC1 gene silencing in SMMC‑7721 cells. (spandidos-publications.com)
  • There was no fold change in caspase-9 gene expression level suggesting that HK-1 cellular apoptosis occurred independent of caspase-9. (nottingham.ac.uk)
  • a Schematic overview of the caspase-8 gene and localization of the caspase 8 polymorphisms of interest. (biomedcentral.com)
  • This is similar to the situation observed in patients with a mutation in caspase-8, who have decreased concentrations of circulating antibodies after antigenic stimulation. (sciencemag.org)
  • Antibodies to p38, phospho-p38 (p-p38), and caspase-8 were purchased from Cell Signaling (USA). (scielo.br)
  • The extent of apoptosis was assessed by the TUNEL method and caspase 3, 6 and 8 expression by immunohistochemistry with specific antibodies. (semanticscholar.org)
  • Moreover, the biochemical form of caspase-8 differed in the two pathways. (wikipedia.org)
  • 2009). Simultaneous Quantitative Monitoring of Drug-induced Caspase Cascade Pathways in Carcinoma Cells. (nexcelom.com)
  • MUC1-CT is involved in numerous signaling pathways, including Wnt/β-catenin ( 4 ), c-Src ( 5 ), Grb2/Sos ( 6 ), glycogen synthase kinase 3 β ( 4 ), epidermal growth factor receptor ( 7 , 8 ) and nuclear factor-κB ( 9 , 10 ), which regulate the processes of cell survival and proliferation. (spandidos-publications.com)
  • Our findings clearly imply that activation of p38 MAPK promotes death receptor-independent activation of caspase-8 and apoptotic cell death pathways, thus providing a novel cellular mechanism for the anticancer activity of sphingolipid metabolites. (aacrjournals.org)
  • Caspase-8 is intimately involved in two essential death pathways-apoptosis and necroptosis-that are responsible for the fate of a cell. (biomedcentral.com)
  • The CaspGLOW™ Fluorescein Active Caspase-8 Staining Kit contains all the reagents necessary to detect active caspase-8 in cells with high sensitivity. (thermofisher.com)
  • Although the genesis of certain tumor types such as neuroblastoma and small-cell lung cancer involves the loss of caspase-8 expression, which suppresses anoikis in neuroblastomas ( 4 ), expression is maintained or increased in most tumor types (Supplementary Fig. S1). (aacrjournals.org)
  • Loss of caspase-8 expression in macrophages promotes onset of a mild systemic inflammatory disease, which is preventable by the deletion of RIPK3. (biomedcentral.com)
  • Further work revealed that caspase-8 was essential for the induction of the transcription factor "nuclear factor κB" (NF-κB) after stimulation through antigen receptors, Fc receptors, or Toll-like receptor 4 in T, B, and natural killer cells. (wikipedia.org)
  • Caspase 8 initiates apoptosis downstream of TNF death receptors by undergoing autocleavage and processing the executioner caspase 3 (ref. 1). (nih.gov)
  • Deletion of caspase-8 inhibited the increase in B cell numbers elicited by agonists for the Toll-like receptors (TLRs), TLR2, -3, and -4. (sciencemag.org)
  • Thus, besides participating in cell death induction by receptors of the TNF/nerve growth factor family, caspase-8, apparently independently of these receptors, also mediates nonapoptotic and perhaps even antiapoptotic activities. (jimmunol.org)
  • Studies of caspase-8 have focused on the ability of this caspase to interact with receptors of the TNF/nerve growth factor (NGF) 5 family and to signal for their cell death-inducing effect ( 10 , 11 , 12 ). (jimmunol.org)
  • Upon stimulation of death receptors such as CD95/APO-1/Fas, TRAIL-R1, TRAIL-R2, TNFR1, and TRAMP, caspase-8 is recruited to the death-inducing signaling complex (DISC). (thermofisher.com)
  • This association with the cell surface death receptors has shown caspase-8 to be a proximal regulator of apoptosis. (ihcworld.com)
  • Interestingly, caspase-8, whose function appeared to be restricted to death receptors, was also activated by these drugs under normal conditions, but not after ATP depletion. (rupress.org)
  • Their activation has been mainly studied upon triggering of death receptors, such as CD95 (Fas/APO-1) and tumor necrosis factor-R1, which recruit caspase-8/FLICE as the most proximal effector to the receptor complex. (semanticscholar.org)
  • Caspase 8 plays a role in APOPTOSIS by cleaving and activating EFFECTOR CASPASES. (curehunter.com)
  • This means affected individuals have a mutation on each of their two caspase-8 alleles. (wikipedia.org)
  • A CYLD substitution mutation at Asp 215 that cannot be cleaved by caspase 8 switches cell survival to necrotic cell death in response to TNF. (nih.gov)
  • These data may provide a mechanism to explain the immunodeficiency in patients with a mutation in caspase-8. (sciencemag.org)
  • Sequencing revealed a homozygous caspase-8 mutation. (aappublications.org)
  • We hypothesize that PGF 2α initiates luteolysis in vivo , at least in part, by increasing the bioactivity or bioavailability of cytokines, such as FasL and that multiple endocrine factors work in concert to activate caspase -3-driven apoptosis during luteolysis. (biomedcentral.com)
  • Caspase-8 is involved in the initiation of the cell death signal cascade. (wikipedia.org)
  • These active enzymes can then cleave other caspases, thereby generating a caspase signaling cascade that leads to a form of programmed cell death termed apoptosis. (thermofisher.com)
  • Fluoride-induced headkidney macrophage cell apoptosis involves activation of the CaMKIIg-ERK 1/2-caspase-8 axis: the role of superoxide in initiating the apoptotic cascade. (fluoridealert.org)
  • Fas and Fas-ligand interact to trigger the caspase cascade, leading to cell apoptosis. (wikipedia.org)
  • Following TNF stimulation, caspase 8 cleaves CYLD to generate a survival signal. (nih.gov)
  • At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. (scbt.com)
  • Prevention of ATP production completely inhibited caspase activation and apoptosis in response to chemotherapeutic drugs and staurosporine. (rupress.org)
  • I am interested in the SiRNA for Human Caspase-8. (abcam.com)
  • Recombinant fragment corresponding to Human Caspase-8 aa 179-385. (abcam.com)
  • Peptide sequence around aa.221~225(T-L-D-K-V) derived from Human Caspase 8. (genetex.com)
  • Sensitivity to ScA was significantly increased among cells expressing caspase 8, whereas siRNA knockdown of caspase 8 increased survival after exposure to ScA. (pnas.org)
  • Consistent with the induction of apoptosis via the lipid compartment, we noted accumulation and aggregation of ceramide in treated cells and subsequent colocalization with caspase 8. (pnas.org)
  • Although vICA is dispensable for viral replication in vitro , the common targeting of caspase-8 activation by diverse herpesviruses argues for an important role for this antiapoptotic mechanism in the pathogenesis of viral infection in the host, most likely in avoiding immune clearance by cytotoxic lymphocytes and natural killer cells. (pnas.org)
  • Here we demonstrate a critical role for caspase-8 in regulating necroptosis of intestinal epithelial cells (IECs) and terminal ileitis. (nih.gov)
  • Here, we show that expression of Casp-8 in endothelial cells (ECs) is required for proper postnatal retina angiogenesis. (jci.org)
  • F ) Quantification of cCasp-3 + IsoB4 + cells per vessel area revealing fewer apoptotic ECs in Casp-8 ECKO retinas compared with Casp-8 WT ( n = 6 WT, n = 7 ECKO). (jci.org)
  • Caspase-8 is known to be important in regulating T cells. (sciencemag.org)
  • Although caspase-8 was not necessary for B cell development, B cells from bcasp8 −/− mice were more resistant to apoptosis stimulated by the death receptor CD95 than were cells from wild-type mice. (sciencemag.org)
  • Although bcasp8 −/− B cells were resistant to apoptosis induced by CD95, the lack of bcasp8 −/− B cell expansion in response to the TLR4 agonist lipopolysaccharide (LPS) was due to increased cell death mediated by caspase-3. (sciencemag.org)
  • Although perhaps best known for its apoptotic functions, caspase-8 acts to mediate TLR signaling and NF-κB activation in B cells. (sciencemag.org)
  • Caspase-8 deletion in bone-marrow cells resulted in arrest of hemopoietic progenitor functioning, and in cells of the myelomonocytic lineage, its deletion led to arrest of differentiation into macrophages and to cell death. (jimmunol.org)
  • However, increasing evidence suggests that the death-inducing caspases, beside their apoptotic role, also have functions that contribute to activities of living cells (see, e.g. (jimmunol.org)
  • f Annexin-V/PI flow cytometry analysis of PC3 empty vector(EV) and procaspase-8(C8) CRISPR cells following pre-treatment for 24 h with 2.5 µM Entinostat(Ent) and a further 24 h with 1 µM TL32711 and 10 ng/mL TNFα combination. (nature.com)
  • In addition, Caspase 8 also reacts with Jurkat cells and Tonsil. (thermofisher.com)
  • In conclusion, our data indicate that p38MAPK and caspase-8 are involved in the process of DATS-induced apoptosis in human CNE2 cells and interact with each other. (scielo.br)
  • In the central control and effective stage of apoptosis, activated caspase-8 can lead directly to the appearance of apoptotic structural characteristics in cells, and play a key role in the process of apoptosis (6,7). (scielo.br)
  • Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. (scbt.com)
  • 3,4 Some cancer therapies in development include molecules that trigger activation of Caspase-8-mediated apoptosis, specifically in cancer cells. (nexcelom.com)
  • The CaspGLOW™Fluorescein Active Caspase-8 Staining Kit offers a simple, sensitive method for detection of active Caspase-8 in living cells. (nexcelom.com)
  • The fluorescent marker (FITC) on the IETD-FMK allows for the detection of cells undergoing apoptosis via Caspase-8 activation using the Vision CBA Analysis System . (nexcelom.com)
  • To determine how a death ligand stimulus is effectively translated into caspase-8 activity, we assessed this activity over time in single cells with compartmentalized probes that are cleaved by caspase-8 and used multiscale modeling to simultaneously describe single-cell and population data with an ensemble of single-cell models. (sciencemag.org)
  • Caspase-3 and caspase-7 have been identified as key executors of apoptosis in mammalian cells and play a central role in the execution phase of apoptosis ( 14 , 15 ). (aacrjournals.org)
  • Herein, we report that pro-caspase-8 is capable of restoring cell migration/adhesion to caspase-8-null cells, establishing the first biological function of a pro-caspase. (aacrjournals.org)
  • Tyrosine-380 was required for the restoration of cell motility and cell adhesion in caspase-8-null cells, demonstrating the importance of the caspase-8-p85 interaction for these nonapoptotic functions. (aacrjournals.org)
  • Caspase-8 has also been implicated in the proinvasive effects of FASL in tumor cells ( 3 ). (aacrjournals.org)
  • However, the pro-caspase-8 form predominates in nonapoptotic cells. (aacrjournals.org)
  • ICT's Caspase-8 FLICA kit was used to detect apoptosis in U937 cells, a histiocytic lymphoma cell line. (immunochemistry.com)
  • FLICA revealed that 41.9% of cells treated with CHX had caspase-8 activity, compared with 19.1% of cells treated with TNF, and only 1.9% of the control cells (Ms. Jennifer Mitchell, Scripps). (immunochemistry.com)
  • Overexpression of dominant-negative caspase-8 interfered with radiation-induced apoptosis, Caspase-8 activation by ionizing radiation was not observed in cells genetically defective for the Src-like tyrosine kinase Lck, Cells lacking Lck also displayed a marked resistance towards apoptosis induction upon ionizing radiation. (diva-portal.org)
  • In contrast, activation-induced cell death of T cells in c-FLIP L Tg mice was unaffected, suggesting that this deletion process can proceed in the absence of active caspase 8. (asm.org)
  • Results: The TF signaling complexes were shown to prevent apoptosis induced by serum starvation and TRAIL in cancer cells by reduced activation of caspase-8 in a PI3k/AKT-dependent manner. (diva-portal.org)
  • 2011). A specific site in IRF3 is targeted by caspase-8, activated in RNA or DNA virus-infected and dsRNA-stimulated cells (Sears et al . (bio-protocol.org)
  • 2011). In the current protocol, we have outlined a simple and detailed procedure to biochemically analyze the proteolysis of IRF3 in virus-infected cells and the specific role of caspase-8 in this process. (bio-protocol.org)
  • In virus-infected cells, IRF3 can be proteolytically cleaved by caspase-8, which gets activated during infection. (bio-protocol.org)
  • Erucylphosphocholine-induced apoptosis in glioma cells: involvement of death receptor signalling and caspase activation. (semanticscholar.org)
  • Similar to other caspases, caspase-8 also exists in cells as an inactive proenzyme. (antibody-antibodies.com)
  • Additionally, scientists have used caspases as cancer therapy to kill unwanted cells in tumours. (wikipedia.org)
  • As cells of the innate immune system, and in particular macrophages, are now at the forefront of autoimmune disease pathogenesis, we examined the potential involvement of caspase-8 within this population. (biomedcentral.com)
  • We previously showed that conditional deletion of Fas or caspase-8 specifically in innate immune cells supports a role for these signaling components in cell activation. (biomedcentral.com)
  • However, PGF 2α or Jo2 at 48 h post-ovulation and collected 8 h later induced caspase -3 activation in 13.2 ± 1.8% and 13.7 ± 2.2 % of the cells, respectively and resulted in 16.35 ± 0.7% (PGF 2α ) and 14.3 ± 2.5% TUNEL-positive cells when compared to 1.48 ± 0.8% of cells CL in IgG treated controls. (biomedcentral.com)
  • The results also indicate that ICD-85-induced apoptosis on HeLa cells is associated with the activation of caspase-8. (ac.ir)
  • Consistent with its role in cell death induction, deletion of caspase-8 in hepatocytes protected them from Fas-induced caspase activation and death. (jimmunol.org)
  • Using mice that specifically ablate caspase-8 auto-processing, I have demonstrated that caspase-8 enzymatic, but not autoprocessing activity, mediates induction of inflammatory cytokines by a wide variety of TLR stimuli. (upenn.edu)
  • Western blot analysis further confirmed that caspase-2 and -8 were activated after induction of HOXA5 expression. (aacrjournals.org)
  • In addition, fisetin triggered the activations of caspases-3 and -8 and the cleavages of poly (ADP-ribose) polymerase, resulting in apoptosis induction. (springer.com)
  • The defect in lymphocyte activation and protective immunity suggested that caspase-8 had additional signaling roles in lymphocytes. (wikipedia.org)
  • Clinical data have suggested both apoptotic and nonapoptotic roles for caspase-8 in human disease. (sciencemag.org)
  • To study B cell-specific roles for caspase-8, Lemmers et al . (sciencemag.org)
  • Because no such phenotype results from targeting of any of the known TNF/NGF family members that use caspase-8 in their signaling, these findings suggested that caspase-8 serves other functional roles as well. (jimmunol.org)
  • Caspases play important roles in apoptosis and inflammation. (immunochemistry.com)
  • The mammalian caspases play distinct roles in apoptosis and inflammation. (celltechnology.com)
  • Caspases have other identified roles in programmed cell death such as pyroptosis and necroptosis . (wikipedia.org)
  • There are other identified roles of caspases such as cell proliferation, tumour suppression, cell differentiation, neural development and axon guidance and ageing. (wikipedia.org)
  • IETD-FMK is cell-permeable and binds irreversibly to active caspase-8 within the cell. (nexcelom.com)
  • These results indicate that expression of HOXA5 can induce apoptosis through an apoptotic mechanism mediated by caspase-2 and -8. (aacrjournals.org)
  • Silencing LC3 by shRNA, or the LC3 mutants defective in membrane localization or p62 interaction fail to induce caspase-8 activation and apoptosis. (asm.org)
  • Anticancer drugs induce caspase-8/FLICE activation and apoptosis in the absence of CD95 receptor/ligand interaction. (semanticscholar.org)
  • article{Wesselborg1999AnticancerDI, title={Anticancer drugs induce caspase-8/FLICE activation and apoptosis in the absence of CD95 receptor/ligand interaction. (semanticscholar.org)
  • Consistent with these findings, real-time polymerase chain reaction (PCR) analysis of the abundance of transcripts of NF-κB target genes showed that deletion of caspase-8 decreased the LPS-induced expression of these genes. (sciencemag.org)
  • I and J ) Representative images of the retinal vasculature stained with IsoB4 in Casp-8 WT /MLKL KO and Casp-8 ECKO /MLKL KO mice. (jci.org)
  • generated mice with B cell-specific inactivation of caspase-8 ( bcasp8 −/− ). (sciencemag.org)
  • Interestingly, mice lacking caspase-8 were severely susceptible to Yersinia infection and had defective pro-inflammatory cytokine production. (upenn.edu)
  • As of 2009, there are 11 or 12 confirmed caspases in humans [note 1] and 10 in mice, carrying out a variety of cellular functions. (wikipedia.org)
  • Wild-type (WT) or caspase -3 deficient female mice, 25-26 days old, were given 10 IU equine chorionic gonadotropin (eCG) intraperitoneally (IP) followed by 10 IU human chorionic gonadotropin (hCG) IP 46 h later to synchronize ovulation. (biomedcentral.com)
  • Regardless of genotype or treatment, CL in ovaries collected from mice injected 24 h after ovulation showed no evidence of active caspase -3 or apoptosis. (biomedcentral.com)
  • In contrast, CL in ovaries collected from caspase -3 deficient mice whether treated with PGF 2α , Jo2, or control IgG at 48 h post-ovulation showed little evidence of active caspase -3 or apoptosis. (biomedcentral.com)
  • CL of WT mice treated with Jo2 at 48 h post-ovulation had an 8-fold increase in the activity of caspase -8, an activator of caspase -3 that is coupled to the FAS death receptor. (biomedcentral.com)
  • Somewhat unexpectedly, however, treatment of WT mice with PGF 2α at 48 h post-ovulation resulted in a 22-fold increase in caspase -8 activity in the CL, despite the fact that the receptor for PGF 2α has not been shown to be directly coupled to caspase -8 recruitment and activation. (biomedcentral.com)
  • Caspases are usually involved in apoptosis and inflammation but they also exhibit nonapoptotic functions. (reactome.org)
  • Activated caspase-8 is able to cleave additional downstream caspases, which include caspase-3, to ultimately elicit the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage. (asm.org)
  • Western blots suggested that the Fas signal was intact up to recruitment of Fas-associated death domain, but that subsequent recruitment of caspase-8 was defective. (aappublications.org)
  • Treatment with TRAIL leads to the recruitment of caspase-8 to the plasma membrane-bound preligand assembly complex for the assembly of a death-inducing signaling complex. (aacrjournals.org)
  • The FLICA reagent FAM-LETD-FMK enters each cell and irreversibly binds to activated caspase-8. (immunochemistry.com)
  • CEDS is caused by homozygous mutations in caspase-8. (wikipedia.org)
  • Caspase-8 mutations lead to a disorder characterized by expansion of lymphocytes in secondary lymphoid organs and abnormal lymphocyte activation. (aappublications.org)
  • v-FLIP resembles caspase 8 in containing two DED but lacks the enzymatic C-terminal portion. (asm.org)
  • Together, these findings provide molecular insights into a key aspect of c-FLIP(L) function that modulates procaspase-8 activation to elicit diverse responses in different cellular contexts. (rcsb.org)
  • Modeling indicated that sustained membrane-bound caspase-8 activity is followed by transient cytosolic activity, which can be interpreted as a molecular timer mechanism reflected by a limited lifetime of active caspase-8. (sciencemag.org)
  • Caspases also have a role in inflammation, whereby it directly processes pro-inflammatory cytokines such as pro-IL1β. (wikipedia.org)