Rare congenital lymphoid disorder due to mutations in certain Fas-Fas ligand pathway genes. Known causes include mutations in FAS, TNFSF6, NRAS, CASP8, and CASP10 proteins. Clinical features include LYMPHADENOPATHY; SPLENOMEGALY; and AUTOIMMUNITY.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Disorders characterized by proliferation of lymphoid tissue, general or unspecified.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
A long pro-domain caspase that contains a death effector domain in its pro-domain region. Activation of this enzyme can occur via the interaction of its N-terminal death effector domain with DEATH DOMAIN RECEPTOR SIGNALING ADAPTOR PROTEINS. Caspase 10 plays a role in APOPTOSIS by cleaving and activating EFFECTOR CASPASES. Several isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Antibodies produced by a single clone of cells.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.
Any technique by which an unknown color is evaluated in terms of standard colors. The technique may be visual, photoelectric, or indirect by means of spectrophotometry. It is used in chemistry and physics. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A long pro-domain caspase that contains a death effector domain in its pro-domain region. Caspase 8 plays a role in APOPTOSIS by cleaving and activating EFFECTOR CASPASES. Activation of this enzyme can occur via the interaction of its N-terminal death effector domain with DEATH DOMAIN RECEPTOR SIGNALING ADAPTOR PROTEINS.
A long pro-domain caspase that contains a caspase recruitment domain in its pro-domain region. Caspase 9 is activated during cell stress by mitochondria-derived proapoptotic factors and by CARD SIGNALING ADAPTOR PROTEINS such as APOPTOTIC PROTEASE-ACTIVATING FACTOR 1. It activates APOPTOSIS by cleaving and activating EFFECTOR CASPASES.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Disorders that are characterized by the production of antibodies that react with host tissues or immune effector cells that are autoreactive to endogenous peptides.
Any of a group of malignant tumors of lymphoid tissue that differ from HODGKIN DISEASE, being more heterogeneous with respect to malignant cell lineage, clinical course, prognosis, and therapy. The only common feature among these tumors is the absence of giant REED-STERNBERG CELLS, a characteristic of Hodgkin's disease.
The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.
A malignant disease characterized by progressive enlargement of the lymph nodes, spleen, and general lymphoid tissue. In the classical variant, giant usually multinucleate Hodgkin's and REED-STERNBERG CELLS are present; in the nodular lymphocyte predominant variant, lymphocytic and histiocytic cells are seen.
A general term for various neoplastic diseases of the lymphoid tissue.
A single nucleotide variation in a genetic sequence that occurs at appreciable frequency in the population.
A group of heterogeneous lymphoid tumors generally expressing one or more B-cell antigens or representing malignant transformations of B-lymphocytes.
A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.
A subclass of peptide hydrolases that depend on a CYSTEINE residue for their activity.
ENDOPEPTIDASES which have a cysteine involved in the catalytic process. This group of enzymes is inactivated by CYSTEINE PROTEINASE INHIBITORS such as CYSTATINS and SULFHYDRYL REAGENTS.
The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.
Exogenous and endogenous compounds which inhibit CYSTEINE ENDOPEPTIDASES.
Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.
Databases devoted to knowledge about specific genes and gene products.
The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
The systematic study of the complete DNA sequences (GENOME) of organisms.

Molecular cloning and characterization of two novel pro-apoptotic isoforms of caspase-10. (1/114)

Caspase-10/a (Mch4) and caspase-10/b (FLICE2) are related death effector domain-containing cysteine aspartases presumed to be at or near the apex of apoptotic signaling pathways. We report the cloning and characterization of two novel proteins that are splice isoforms of the caspase-10 family. Caspase-10/c is a truncated protein that is essentially a prodomain-only form of the caspase that lacks proteolytic activity in vitro but efficiently induces the formation of perinuclear filamentous structures and cell death in vivo. Caspase-10/c mRNA is specifically up-regulated upon TNF stimulation, suggesting a potential role of this isoform in amplifying the apoptotic response to extracellular stimuli such as cytokines. Caspase-10/d is a hybrid of the known caspases Mch4 and FLICE2, as it is identical to FLICE2 except for the small (p12) catalytic subunit, which is identical to Mch4. Caspase-10/d is proteolytically active in vitro and also induces cell death in vivo, although it is less active than Mch4. The mRNAs for all known isoforms of caspase-10 are abundantly expressed in fetal lung, kidney, and skeletal muscle but are very poorly expressed or absent in these tissues in the adult, implying a possible role for the caspase-10 family in fetal development.  (+info)

Impairment of TNF-receptor-1 signaling but not fas signaling diminishes T-cell apoptosis in myelin oligodendrocyte glycoprotein peptide-induced chronic demyelinating autoimmune encephalomyelitis in mice. (2/114)

T-cell apoptosis in inflammatory demyelinating lesions of chronic myelin oligodendrocyte glycoprotein peptide35-55 induced autoimmune encephalomyelitis was studied in several different gene knockout mice as well as their wild-type counterparts. The gene deletions included tumor necrosis factor (TNF) alpha, lymphotoxin, TNF receptor 1 or 2, Fas-L, inducible nitric oxide synthase, perforin, and interleukin1beta-converting enzyme. Impairment of the TNF receptor 1 pathway led to a 50% reduction of T-cell apoptosis in the central nervous system lesions, whereas the other genetic deletions showed no significant effect. Our study thus identified the TNF receptor 1 signaling pathway as one mechanism responsible for the removal of T lymphocytes from inflammatory demyelinating lesions of the central nervous system.  (+info)

Caspases in T-cell receptor-induced thymocyte apoptosis. (3/114)

Apoptosis eliminates inappropriate or autoreactive T lymphocytes during thymic development. Intracellular mediators involved in T-cell receptor (TCR)-mediated apoptosis in developing thymocytes during negative selection are therefore of great interest. Caspases, cysteine proteases that mediate mature T-cell apoptosis, have been implicated in thymocyte cell death, but their regulation is not understood. We examined caspase activities in distinct thymocyte subpopulations that represent different stages of T-cell development. We found caspase activity in CD4+CD8+ double positive (DP) thymocytes, where selection involving apoptosis occurs. Earlier and later thymocyte stages exhibited no caspase activity. Only certain caspases, such as caspase-3 and caspase-8-like proteases, but not caspase-1, are active in DP thymocytes in vivo and can be activated when DP thymocytes are induced to undergo apoptosis in vitro by TCR-crosslinking. Thus, specific caspases appear to be developmentally regulated in thymocytes.  (+info)

Inherited human Caspase 10 mutations underlie defective lymphocyte and dendritic cell apoptosis in autoimmune lymphoproliferative syndrome type II. (4/114)

Caspases are cysteine proteases that mediate programmed cell death in phylogenetically diverse multicellular organisms. We report here two kindreds with autoimmune lymphoproliferative syndrome (ALPS) type II, characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects, that harbor independent missense mutations in Caspase 10. These encode amino acid substitutions that decrease caspase activity and interfere with death receptor-induced apoptosis, particularly that stimulated by Fas ligand and TRAIL. These results provide evidence that inherited nonlethal caspase abnormalities cause pleiotropic apoptosis defects underlying autoimmunity in ALPS type II.  (+info)

Structure, expression, and function of the Xenopus laevis caspase family. (5/114)

Caspases, a family of cysteine proteases, have been recognized as the central executors of programmed cell death. Nonetheless, the information on the caspase family has been limited to mammals, Drosophila, and nematodes. To examine the structure and characterization of the Xenopus caspase family, we have cloned the cDNAs encoding caspase-2 and -6-10 in addition to caspase-1 and -3, which we characterized previously (Yaoita, Y., and Nakajima, K. (1997) J. Biol. Chem. 272, 5122-5127). First, the existence of these caspases in frog suggests that the caspase cascades clarified in mammals are conserved at least from Amphibia. Interestingly, Xenopus caspase-1, -8, and -10 (especially caspase-8) showed a lower degree of identity to human equivalents than the other caspases. Second, mRNAs of many caspases increased during the climax of metamorphosis in regressing organs, tail, and intestine, where programmed cell death occurs, but not in apoptotic tail-derived cultured cells (XLT-15-11) treated with thyroid hormone, showing that new RNA synthesis of caspases is dispensable to programmed cell death. Third, comparison of human and Xenopus caspase sequences implies that some proposed regulations of human caspases are not conserved in frog.  (+info)

Activation of the NF-kappaB pathway by caspase 8 and its homologs. (6/114)

Caspase 8 is the most proximal caspase in the caspase cascade and has been known for its role in the mediation of cell death by various death receptors belonging to the TNFR family. We have discovered that Caspase 8 can activate the NF-kappaB pathway independent of its activity as a pro-apoptotic protease. This property is localized to its N-terminal prodomain, which contains two homologous death effector domains (DEDs). Caspase 10 and MRIT, two DEDs-containing homologs of Caspase 8, can similarly activate the NF-kappaB pathway. Dominant-negative mutants of the Caspase 8 prodomain can block NF-kappaB induced by Caspase 8, FADD and several death receptors belonging to the TNFR family. Caspase 8 can interact with multiple proteins known to be involved in the activation of the NF-kappaB pathway, including the serine-threonine kinases RIP, NIK, IKK1 and IKK2. Thus, DEDs-containing caspases and caspase homolog(s) may have functions beyond their known role in the mediation of cell death. Oncogene (2000) 19, 4451 - 4460.  (+info)

Resistance to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in neuroblastoma cells correlates with a loss of caspase-8 expression. (7/114)

Disruption of apoptotic pathways may be involved in tumor formation, regression, and treatment resistance of neuroblastoma (NB). Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a potent inducer of apoptosis in cancer cell lines, whereas normal cells are not sensitive to TRAIL-mediated apoptosis. In this study we analyzed the expression and function of TRAIL and its agonistic and antagonistic receptors as well as expression of cellular FLICE-like inhibitory protein and caspase-2, -3, -8, -9, and -10 in 18 NB cell lines. Semiquantitative RT-PCR revealed that TRAIL-R2 and TRAIL-R3 are the main TRAIL-receptors used by NB cells. Sensitivity to TRAIL-induced apoptosis did not correlate with mRNA expression of TRAIL receptors or cellular FLICE-like inhibitory protein. Surprisingly, caspase-8 and caspase-10 mRNA expression was detected in only 5 of 18 NB cell lines. Interestingly, only these five NB cell lines were susceptible to TRAIL-induced apoptosis in a time- and dose-dependent manner. Treatment with 5-aza-2'-deoxycytidine restored mRNA and protein expression of caspase-8 and TRAIL sensitivity of resistant cell lines, suggesting that gene methylation is involved in caspase inactivation. The TRAIL system seems to be functional in NB cells expressing caspase-8 and/or caspase-10. Because many cytotoxic drugs induce caspase-dependent apoptosis, failure to express caspase-8 and/or caspase-10 might be an important mechanism of resistance to chemotherapy in NB.  (+info)

Progressive resistance to apoptosis in a cell lineage model of human proliferative breast disease. (8/114)

BACKGROUND: Proliferative breast disease (PBD) may increase a woman's risk of developing breast cancer, perhaps by decreasing cellular sensitivity to apoptosis. To determine whether resistance to apoptosis develops during PBD, we investigated apoptosis initiated through the Fas pathway in a series of cell lines that recapitulates the morphologic changes of PBD in nude/beige mice. METHODS: The series of cell lines used was MCF-10A cells (parental preneoplastic human breast epithelial cells), MCF-10AT cells (transformed with T(24) Ha-ras), and MCF-10ATG3B cells (derivative cells that progress to carcinoma). Fas-mediated apoptosis, induced when a Fas monoclonal antibody bound to and activated the Fas receptor on these cells, was assessed morphologically and by flow cytometry. Levels of proteins involved in Fas-mediated apoptosis and cleavage of poly(adenosine diphosphate-ribose) polymerase (PARP), an end product of caspase activation, were determined by immunoblotting. Bcl-2 and Bax heterodimerization was examined by coimmunoprecipitation. All statistical tests were two-sided. RESULTS: Sensitivity to Fas-mediated apoptosis decreased with the tumorigenic potential of cells: MCF-10A cells were extremely susceptible, MCF-10AT cells were less susceptible, and MCF-10ATG3B cells were resistant. The percentage of apoptotic cells declined, from 24% to 8% to 6%, respectively. All lines produced Fas ligand (FasL) and had comparable levels of Fas receptor, FasL, Fas-associated death-domain protein, and caspases 3 and 6. Levels of caspase 8 were similar in MCF-10A and MCF-10AT cells but about 30% lower in MCF-10ATG3B cells (P>.01 but <.05). Levels of caspase 10 were about 20% lower in MCF-10AT cells (P>.005 but <.01) and about 59% lower in MCF-10ATG3B cells than in MCF-10A cells (P>.01 but <.05). PARP cleavage was detected in MCF-10A and MCF-10AT cells but not in MCF-10ATG3B cells. Levels of Bax, Bid, and Bak proteins were similar in all lines, but levels of Bcl-2 were lower in MCF-10AT and MCF-10ATG3B cells than in MCF-A cells, and Bcl-2-Bax heterodimerization progressively declined in the series. CONCLUSION: Resistance to Fas-mediated apoptosis appears to develop progressively in the MCF-10AT cell series.  (+info)

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Bax is a Bcl-2 protein crucial for apoptosis initiation and execution, whose active conformation is only partially understood. Dipolar EPR spectroscopy has proven to be a valuable tool to determine coarse-grained models of membrane-embedded Bcl-2 proteins. Here we show how the combination of spectroscopically distinguishable nitroxide and gadolinium spin labels and Double Electron-Electron Resonance can help to gain new insights into the quaternary structure of active, membrane-embedded Bax oligomers. We show that attaching labels bulkier than the conventional MTSL may affect Bax fold and activity, depending on the protein/label combination. However, we identified a suitable pair of spectroscopically distinguishable labels, which allows to study complex distance networks in the oligomers that could not be disentangled before. Additionally, we compared the stability of the different spin-labeled protein variants in E. coli and HeLa cell extracts. We found that the gem-diethyl nitroxide-labeled Bax
Mouse anti Human caspase-14, clone 4C9 recognizes human caspase-14, a 242 amino acid ~28 kDa non-apoptotic caspase which exists as a
Complete information for CASP5 gene (Protein Coding), Caspase 5, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
The quake in northeastern Italy destroyed more than 300 000 wheels of Parmesan and Grana Padano, a similar cheese, with an estimated value of more than €250m.
BioAssay record AID 513048 submitted by ChEMBL: Inhibition of human caspase-3-mediated apoptosis assessed as Ac-DEVD-7-amino-4-methylcoumarin cleavage product at 100 uM by fluorescence assay.
CASP1 - CASP1 (untagged)-Human caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) (CASP1), transcript variant gamma available for purchase from OriGene - Your Gene Company.
CASP5 - CASP5 (untagged)-Human caspase 5, apoptosis-related cysteine peptidase (CASP5), transcript variant d available for purchase from OriGene - Your Gene Company.
Autoimmune lymphoproliferative syndrome (ALPS) is characterized by nonmalignant lymphadenopathy, splenomegaly, and autoimmune cytopenias. In 1995, defective lymphocyte apoptosis secondary to mutations in the FAS gene was identified as a molecular basis for ALPS.
The purpose of the protocol is to allow for patients, and relatives of patients, who may have the newly described autoimmune lymphoproliferative syndrome, to be evaluated at the NIH Clinical Center. This evaluation will include blood and relevant tissue studies along with long-term clinical evaluations to define the biology, inheritance,clinical spectrum, and natural history of this syndrome. The aim of the research is to understand mechanisms involved in the development of expanded numbers of what is typically a rare population of immune cells (CD4-8-/TCRalpha/beta+ T cells, otherwise referred to as double negative T cells), and how these relate to the development of expanded numbers of immune cells and autoimmune (self against self) responses in patients with ALPS.. In some cases, we may proivide treatment related to ALPS. These treatments are consistent with standard medical practice.. Participants with ALPS will be invited to visit the NIH once a year or more frequently when clinically ...
AUTOIMMUNE LYMPHOPROLIFERATIVE SYNDROME; ALPS description, symptoms and related genes. Get the complete information in our medical search engine for p
Langan RC, Gill F, Raiji MT, Mullinax JE, Pittaluga S, Pandalai P, Davis J, Perkins K, Avital I, Rudloff U. Autoimmune pancreatitis in the autoimmune lymphoproliferative syndrome (ALPS): a sheep in wolves clothing? Pancreas. 2013 Mar; 42(2):363-6 ...
The nomenclature for the various types of ALPS is determined based on the genetic mutation present in an individual. Patients meeting diagnostic criteria for ALPS in whom no genetic mutation can be id... more
Although specific mutations that cause ALPS have been identified, no environmental exposures or risk factors have yet been associated with an increased prevalence of the disease. Certainly, any patien... more
A fundamental biological question that remains largely unresolved concerns the mechanism by which binding of ligands to receptors on the cell surface causes transmission of a signal through the plasma membrane. One appealing explanation has been that ligand binding brings receptors together into multimeric complexes. Three reports describe cases in which the opposite approach is taken, and the receptors are bound and lie in wait for the ligand. Siegel et al. and Chan et al. have examined how the Fas and tumor necrosis factor (TNF) receptors signal. They define a protein interaction domain in these receptors that mediates assembly of the receptors into complexes in the absence of ligand. Such association is shown to be necessary for ligand binding and subsequent signaling. The results also explain how abnormal forms of Fas can dominantly prevent Fas-induced signaling in the human disease known as autoimmune lymphoproliferative syndrome. When bound to their cognate receptors, interferons (IFNs) ...
Free Online Library: Programmed Death Ligand-1 (PD-L1) Expression in the Programmed Death Receptor-1 (PD-1)/PD-L1 Blockade: A Key Player Against Various Cancers.(Report) by Archives of Pathology & Laboratory Medicine; Health, general Apoptosis Research Gene expression
Summary of CASP8 (Casp-8, FLICE, MACH, MCH5) expression in human tissue. Cytoplasmic expression of varying intensity in most tissues.
Treatment is most commonly directed at autoimmune disease and may be needed to treat bulky lymphoproliferation. First line therapies include corticosteroids (very active but toxic with chronic use), and IVIgG, which are not as effective as in other immune cytopenia syndromes. Second line therapies include: mycophenolate mofetil (cellcept)[15] which inactivates inosine monophosphate, most studied in clinical trials with responses varying (relapse, resolution, partial response). It does not affect lymphoproliferation or reduce DNTs, with no drug-drug interactions. This treatment is commonly used agent in patients who require chronic treatment based on tolerance and efficacy. It may cause hypogammaglobulinemia (transient) requiring IVIgG replacement. Sirolimus (rapamycin, rapamune) which is a mTOR (mammalian target of rapamycin) inhibitor[16] can be active in most patients and can in some cases lead to complete or near-complete resolution of autoimmune disease (,90%)[17][18] With this treatment ...
Logical Images, Inc. d/b/a VisualDx (hereinafter VisualDx, we, us, or our) has created this Acceptable Use Policy, Medical Disclaimer, & Copyright Notice (this Notice) to inform you (hereinafter you, your, or yourself) as a purchaser of a license for and/or user of the software hosted by VisualDx known as VisualDx (the Software) of certain important terms and conditions set forth in the VisualDx End User License Agreement that governs your license for and/or use of the Software (the EULA). This Notice is subject to all of the terms and conditions set forth in the EULA and does not replace or limit it in anyway. You should read the EULA in detail prior to purchasing a license for or using the Software to make sure you understand and agree to its terms and conditions. Nothing in this Notice will (a) expand your rights or VisualDx′s obligations under the EULA or (b) modify or otherwise affect any terms and conditions of the EULA or the rights of the parties under the EULA. In ...
Pain management information for pain medicine healthcare professionals in treating and caring for their patients. Clinical Pain Advisor offers news, case studies and more.
Covered on Genetics Home Reference: autoimmune lymphoproliferative syndromebreast cancerFrom NCBI Gene: Caspase-8 deficiencyHepatocellular carcinomaFamilial cancer of breastLung cancerFrom UniProt: Caspase-8 deficiency (CASP8D): Disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization. [MIM:607271] From NCBI Gene: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of ...
Caspase-10 is an enzyme that, in humans, is encoded by the CASP10 gene. This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene. Caspase 10 has been shown to interact with FADD, CFLAR, Caspase 8, Fas receptor, RYBP, TNFRSF1A and TNFRSF10B. The Proteolysis Map Caspase GRCh38: Ensembl release 89: ENSG00000003400 - Ensembl, May 2017 Human PubMed ...
This study will evaluate the safety and effectiveness of an antibiotic called Fansidar on autoimmune lymphoproliferative syndrome (ALPS). Patients with ALPS have enlarged lymph glands, spleen and/or liver, abnormal blood cell counts and overactive immune function. Current treatments are aimed at suppressing the immune system and improving symptoms, such as anemia (low red blood cell count) and low white blood cell and platelet counts. These treatments, however, are only partially effective and may have complications. Fansidar is a combination of two drugs, sulfadoxine and pyrimethamine, that is used to treat or prevent parasitic infections such as malaria. Recently a child with ALPS who was treated with Fansidar for a different illness had a marked shrinkage of the lymph organs. This study will examine whether Fansidar can shrink the lymph glands or spleen in patients with ALPS.. Patients with ALPS between the ages of 4 and 70 years who have had lymph gland enlargement for at least 1 year and ...
Teachey DT, Manno CS, Axsom KM, et al: Unmasking Evans syndrome: T-cell phenotype and apoptotic response reveal autoimmune lymphoproliferative syndrome (ALPS). Blood 2005;105:2443-2448. Bader-Meunier B, Rieux-Laucat F, Croisille L, et al: Dyserythropoiesis associated with a fasdeficient condition in childhood. Br J Haematol 2000;108:300-304. Pensati L, Costanzo A, Ianni A, et al: Fas/Apo1 mutations and autoimmune lymphoproliferative syndrome in a patient with type 2 autoimmune hepatitis. Gastroenterology 1997;113: 1384-1389. Cell 1997;89:1067-1076. Stanger B, Leder P, Lee T, Kim E, Seed B: RIP: A novel protein containing a death domain that interacts with fas/APO-1(CD95) in yeast and causes cell death. Cell 1995;81:513-523. Chu K, Niu X, Williams LT: A Fas-associated protein factor, FAF1, potentiates Fas-mediated apoptosis. Proc Natl Acad Sci USA 1995;92:11894-11898. Barnhart BC, Alappat EC, Peter ME: The CD95 type I/type II model. Semin Immunol 2003;15: 185-193. Siegel RM, Muppidi JR, Sarker M, ...
Caspases are cysteine proteases that mediate programmed cell death in phylogenetically diverse multicellular organisms. We report here two kindreds with autoimmune lymphoproliferative syndrome (ALPS) type II, characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory d …
Human caspase 2 ELISA kit can be used for detecting in vitro quantitative levels of caspase-2 (CASP2) in human serum, cell culture supernatant, plasma, tissue
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Expression of CASP8 (Casp-8, FLICE, MACH, MCH5) in soft tissue 2 tissue. Antibody staining with HPA001302, HPA005688 and CAB002047 in immunohistochemistry.
zVAD-fmk does not totally abrogate FasL-triggered apoptosis in HeLa cells expressing caspase-10 at low level.A, B, Casp10+ and Casp10- HeLa cells were incubated
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09:39, 30 April 2015 Homo sapiens:Apoptosis Modulation and Signaling‎ (Corrected ID for AIFM1,CASP4,CASP2,CASP1,HSPA1A,PIDD,TP53,PRKD1,NAIP,XIAP,AIFM2,RIPK1 and PEA15 genes) ...
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Although ALPS is frequently caused by mutations in known genes, such as FAS, FASLG or CASP10, in 20-30% of cases the defect is still unknown. It is highly likely that defects in or overexpression of regulators of these genes such as miR-146a22 could result in an ALPS-like phenotype and account for a not yet defined percentage of ALPS-U cases. In the present study we identified an IL12RB1 mutation and the IL12 signaling pathway as such an alternative cause of an ALPS-like phenotype through regulation of FasL expression. Previously it was shown that activation of T and NK cells by IL12 results in upregulation of FasL.23-26,28,42 For instance, Yu et al. showed that dendritic cell-derived IL12 is involved in upregulation of FasL on NK cells leading to cell death.25 Moreover, in the absence of antigen, IL12 induces apoptosis of T cells via upregulation of FasL which can be blocked by anti-FasL antibodies.26 In line with this, we found that primary human T cells deficient in FasL expression were ...
Complete information for CASP8AP2 gene (Protein Coding), Caspase 8 Associated Protein 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Antho 50 induces caspase 3 activation and UHRF1 down-regulation independently of p53 and p73.B CLL cells were incubated with Antho 50 at 75 μg/mL for the ind
Fas and Fas-ligand interact to trigger the caspase cascade, leading to cell apoptosis. Patients with ALPS have a defect in this ... Archived from the original on 2012-04-26.[unreliable medical source?] Van Der Werff Ten Bosch, Jutte; Schotte, Peter; Ferster, ... 20% of patients CEDS: Caspase 8 deficiency state. No longer considered a subtype of ALPS but distinct disorder RALD: NRAS, KRAS ... 2003 nomenclature IA - Fas IB - Fas ligand IIA - Caspase 10 IIB - Caspase 8 III - unknown IV - Neuroblastoma RAS viral oncogene ...
There are two types of caspases: initiators and effectors. Initiator caspases cleave inactive forms of effector caspases. This ... This pathway controls the activation of caspase-9 by regulating the release of cytochrome c from the mitochondrial ... Caspases (cysteine-aspartic acid proteases) cleave at very specific amino acid residues. ... each leading to the activation of caspase-9. The nucleus and Golgi apparatus are other organelles that have damage sensors, ...
... is a distinctive feature of DNA degraded by caspase-activated DNase (CAD), which is a key event during apoptosis ... Apoptotic DNA fragmentation Caspase-activated DNase Nicoletti assay TUNEL assay Wyllie AH (1980-04-10). "Glucocorticoid-induced ... caspase-3 activity by zinc supplementation in cultured HeLa cells". The Indian Journal of Medical Research. 129 (5): 579-586. ...
It also selectively inhibits effector caspases 2, 3, 6, and 7 but not caspases 8 and 10. This compound has been shown to block ...
He found mutations in the genes encoding Fas and Fas ligand, as well as caspase-10 and N-Ras, are associated with the disorder ... 10 (5): 115, PMC 2438277, PMID 18596934CS1 maint: uses authors parameter (link) John F. Enders Lectureship, Infectious Diseases ...
Kanneganti's lab showed compensatory roles for NLRP3/caspase-1 and caspase-8 in the regulation of IL-1β production in ... Her lab identified caspase-8 and FADD as expression and activation regulators of both the canonical and non-canonical NLRP3 ... This finding went against the dogma that existed at that time that caspase-8 and FADD were involved only in the apoptotic ... This study demonstrated that the NLRP3 inflammasome/pyroptotic pathway is closely connected to the caspase-8-mediated ...
There are two types of caspases: initiators and effectors. Initiator caspases cleave inactive forms of effector caspases. This ... 115 (10): 2610-7. doi:10.1172/JCI26321. PMC 1236695. PMID 16200193.. *^ a b c d e f g h i Caccamo D, et al. (2010). "Critical ... Pharmacological approaches involve inhibitors of caspase activity, and caspase inhibition might delay cell death in the ... This pathway controls the activation of caspase-9 by regulating the release of cytochrome c from the mitochondrial ...
... in a mechanism regulated by caspase-2. She received the Basic Science Research Mentoring Award from the Duke School of Medicine ... "Metabolic regulation of oocyte cell death through the CaMKII-mediated phosphorylation of caspase-2". Cell. 123 (1): 89-103. doi ...
TNF-related apoptosis-inducing ligand TRAIL activates Caspase 3 (CASP3), which in turn interacts with caspases 6, 7, 8, 9, and ... doi:10.1016/S1472-6483(10)61304-1. PMID 16102296.[permanent dead link] Manabe N, Matsuda-Minehata F, Goto Y, et al. (July 2008 ... 10 to induce apoptosis in granulosa cells. In addition, two intracellular inhibitor proteins, cellular FLICE-like inhibitory ...
The function of caspase 4 is not fully known, but it is believed to be an inflammatory caspase, along with caspase 1, caspase 5 ... The Proteolysis Map Caspase Martinon F, Tschopp J (2007). "Inflammatory caspases and inflammasomes: master switches of ... Caspase 4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue (LEVD-), and belongs to a family ... Smith C, Soti S, Jones Torey A, Nakagawa A, Xue D, and Yin H (2017). "NSAIDs are Caspase Inhibitors". Cell Chem Biol. 24 (3): ...
It is an inflammatory caspase, along with caspase 1, caspase 4 and the murine caspase 4 homolog caspase 11, and has a role in ... The Proteolysis Map Caspase Martinon F, Tschopp J (2007). "Inflammatory caspases and inflammasomes: master switches of ... Caspase 5 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue, and belongs to a family of ... 14 (1): 10-22. doi:10.1038/sj.cdd.4402038. PMID 16977329. The MEROPS online database for peptidases and their inhibitors: ...
Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive ... This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene ... Caspase 8, Fas receptor, RYBP, TNFRSF1A and TNFRSF10B. The Proteolysis Map Caspase GRCh38: Ensembl release 89: ENSG00000003400 ... Caspase-10 is an enzyme that, in humans, is encoded by the CASP10 gene. This gene encodes a protein that is a member of the ...
Caspase-independent apoptosis[edit]. The characterization of the caspases allowed the development of caspase inhibitors, which ... Caspases. Caspases play the central role in the transduction of ER apoptotic signals. Caspases are proteins that are highly ... The apoptosome cleaves the pro-caspase to its active form of caspase-9, which in turn cleaves and activates pro-caspase into ... There are two types of caspases: initiator caspases, caspase 2,8,9,10,11,12, and effector caspases, caspase 3,6,7. The ...
Caspase-8 is a caspase protein, encoded by the CASP8 gene. It most likely acts upon caspase-3. CASP8 orthologs[5] have been ... Apoptosis & Caspase 8-The Proteolysis Map (animation). *Caspase 8 at the US National Library of Medicine Medical Subject ... Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive ... 1f9e: CASPASE-8 SPECIFICITY PROBED AT SUBSITE S4: CRYSTAL STRUCTURE OF THE CASPASE-8-Z-DEVD-CHO ...
Caspase-3 can further activate other molecules of procaspase-3 in the cell, causing an exponential increase in caspase-3 ... This allows procaspase-3 to be an active enzyme, and it can then cleave another molecule of procaspase-3 to active caspase-3. ... In cells, the executioner protein, caspase-3, is stored in its inactive form, procaspase-3. This way, the cell can quickly ... Putinski C, Abdul-Ghani M, Stiles R, Brunette S, Dick SA, Fernando P, Megeney LA (2013). "Intrinsic-mediated caspase activation ...
2006). "PPT1 deficiency leads to the activation of caspase-9 and contributes to rapid neurodegeneration in INCL". Hum Mol Genet ... This homeostasis-altering event leads to increased mitochondrial membrane permeability and subsequent activation of caspase-9, ... 1762 (10): 850-6. doi:10.1016/j.bbadis.2006.06.010. PMID 16908122.[permanent dead link] synd/7 at Who Named It? C. Stengel. ... 15 (10): 1586-90. doi:10.1093/hmg/ddl078. PMID 16571600. Late Infantile NCL (LINCL or Jansky-Bielschowsky), on the other hand, ...
Martinon F, Burns K, Tschopp J (2002). "The inflammasome: a molecular platform triggering activation of inflammatory caspases ... first 10=. (도움말); 지원되지 않는 변수 무시됨: ,last 13=. (도움말); 지원되지 않는 변수 무시됨: ,first 12=. (도움말); 지원되지 않는 변수 무시됨: ,first 15=. (도움말); 지원되지 ... last 10=. (도움말); 지원되지 않는 변수 무시됨: ,first 11=. (도움말) *↑ Ferrari S, Di Iorio E, Barbaro V, Ponzin D, Sorrentino FS, Parmeggiani F ... "DICER1/Alu RNA dysmetabolism induces Caspase-8-mediated cell death in age-related macular degeneration". 》PNAS》 111 (45): 16082 ...
These caspases then activate the effector caspases 3, 6, and 7, leading to cleavage of death substrates such as lamin A, lamin ... granzymes enter the cytoplasm of the target cell and their serine protease function triggers the caspase cascade, which is a ... 10 (1): 5396. doi:10.1038/s41467-019-13385-x. PMC 6881447. PMID 31776337. Bakshi RK, Cox MA, Zajac AJ (2014). "Cytotoxic T ... October 2016). "CD8+ T Cells and Endogenous IL-10 Are Required for Resolution of Chemotherapy-Induced Neuropathic Pain". The ...
Paracaspase has been shown to have proteolytic activity through its caspase-like domain in T lymphocytes. Cysteine 464 and ... two ancient families of caspase-like proteins, one of which plays a key role in MALT lymphoma". Mol. Cell. 6 (4): 961-7. doi: ... two central immunoglobulin-like domains involved in the binding to the B-cell lymphoma 10 (Bcl10) protein and a caspase-like ...
Releasing of cytochrome c from the IMS to the cytosol activates procaspases and triggers a caspase cascade leading to apoptosis ... The intermembrane space (IMS) of the chloroplast is exceedingly small, from 10-20 nm thick. Unlike the IMS of the mitochondria ... doi:10.1007/978-981-10-7757-9_7. ISBN 978-981-10-7756-2. PMID 29464561. Cite journal requires ,journal= (help) Jarvis P, Soll J ...
Executioner Caspases (Caspase 3, Caspase 6 and Caspase 7). Once initiator caspases are activated, they produce a chain reaction ... Caspase-1, Caspase-4, Caspase-5 and Caspase-11 are considered 'Inflammatory Caspases'.[7] ... Caspase-1, Caspase-4 and Caspase-5 in humans, and Caspase-1 and Caspase-11 in mice play important roles in inducing cell death ... or direct activation of Executioner Caspases (Caspase 3, Caspase 6 and Caspase 7) to degrade cellular components as shown in ...
In each case, caspase 9 activation leads to the activation of a full caspase cascade and subsequent cell death. It has been ... This functional apoptosome then can provide a platform activation of caspase 9. Caspase 9 exists as a zymogen in the cytosol ... In addition, several other molecules, most notably caspase-3, have been reported to co-purify with the apoptosome and caspase-3 ... Another targeted molecule for cancer therapy involves the caspase family and their regulators. The inhibition of caspase ...
varian caspase recruitment domain (CARD15) dan reseptor interleukin-23 (IL23R) atau polimorfisme autophagy-related 16-like 1 ( ... "2013-10-13. Diakses tanggal 16 September 2014.. *^ Braat H, Peppelenbosch MP, Hommes DW (August 2006). "Immunology of Crohn's ... July 10, 2013. Diakses tanggal 12 June 2014.. *^ Coskun M. Intestinal epithelium in inflammatory bowel disease. Front Med ( ... Epub 2015 Feb 10. *^ Zilberman L, Maharshak N, Arbel Y, Rogowski O, Rozenblat M, Shapira I, Berliner S, Arber N, Dotan I. ...
"PCI-24781 induces caspase and reactive oxygen species-dependent apoptosis through NF-kappaB mechanisms and is synergistic with ... Exerts Cytotoxicity and Histone Alterations via Caspase-8 and FADD in Leukemia Cells". International Journal of Cell Biology. ... 15 (10): 3354-65. doi:10.1158/1078-0432.CCR-08-2365. PMC 2704489. PMID 19417023. Lopez, G; Liu, J; Ren, W; Wei, W; Wang, S; ... 15 (10): 3472-83. doi:10.1158/1078-0432.CCR-08-2714. PMID 19417021. Rivera-Del Valle, N; Gao, S; Miller, CP; Fulbright, J; ...
... and caspase 14. In humans, it is estimated that keratinocytes turn over from stem cells to desquamation every 40-56 days, ... 10 (4): 777-80. doi:10.1093/carcin/10.4.777. PMID 2702726. Pillai S, Bikle DD (January 1991). "Role of intracellular-free ... 1170 (1): 7-10. Bibcode:2009NYASA1170....7K. doi:10.1111/j.1749-6632.2009.04363.x. PMC 2861991. PMID 19686098. Halprin KM ( ... 124 (10): 1681-1690. doi:10.1242/jcs.077552. PMC 3183491. PMID 21511732. Rheinwald, JG; Green, H (1975). "Serial cultivation of ...
One novel protein (ProAgio) has been designed to bind at an unusual site, and then induces apoptosis by recruiting caspase 8. ... Fibronectin domain 10 contains the RGD motif that αVβ3 recognizes. A high-affinity, pure antagonist mutant has been discovered ...
September 2008). "The familial Mediterranean fever protein, pyrin, is cleaved by caspase-1 and activates NF-kappaB through its ... In healthy individuals, pyrin-mediated inflammasome assembly (which leads to the caspase 1) dependent processing and secretion ... it is a protein that binds to the adaptor ASC and the pro form of the enzyme caspase-1 to generate multiprotein complexes ... 8 (4): 307-10. doi:10.1038/sj.ejhg.5200446. PMID 10854115. Gershoni-Baruch R, Shinawi M, Leah K, Badarnah K, Brik R (August ...
... are directly responsible for the expression of anergy associated genes such as ubiquitin ligase GRAIL or a protease caspase 3. ... 10 (6): 640-5. doi:10.1016/s0952-7915(98)80082-4. Tone, Yiukiko; Furuuchi, Keiji; Kojima, Yoshitsugu; Tykocinski, Mark L.; ... Anergized cells tend to produce antiinflammatory IL-10 instead. There are 3 NFAT proteins in the T-cell, NFAT1, NFAT2 and NFAT4 ... doi:10.1182/blood-2009-10-233585. PMID 20103781. Müller, Nicole; Schneider, Th; Zeitz, M; Marth, Th (2001). "Whipple's disease ...
... of the presenilin 1/beta-catenin interaction and preservation of the heterodimeric presenilin 1 complex following caspase ... 4 (10): 1177-81. doi:10.1038/2673. PMID 9771752.. *^ Kashiwa A, Yoshida H, Lee S, Paladino T, Liu Y, Chen Q, Dargusch R, ... 10: 373-403. doi:10.1146/annurev.cb.10.110194.002105. PMID 7888181.. *^ Laudon H, Hansson EM, Melén K, Bergman A, Farmery MR, ... 10 (3): 563-8. doi:10.1097/00001756-199902250-00022. PMID 10208590.. *^ Zhang W, Han SW, McKeel DW, Goate A, Wu JY (February ...
CASP16P: encoding protein Caspase 16, pseudogene. *CCDC113: encoding protein Coiled-coil domain-containing protein 113 ... Miller, David T; Nasir, Ramzi; Sobeih, Magdi M; Shen, Yiping; Wu, Bai-Lin; Hanson, Ellen (2011-10-27). 16p11.2 Microdeletion. ...
... condensed apoptotic nuclei and a 2-4 fold increase in cortical precursors that stained immunopositive for cleaved caspase-3.[30 ... 10 (6): 702-11. doi:10.1038/nn1903. PMID 17515902. S2CID 6486137.. *^ Penzes P, Johnson RC, Sattler R, Zhang X, Huganir RL, ... 107 (4): 306-10. doi:10.1002/ajmg.10133. PMID 11840487.. *^ Zimmer P, Mierau A, Bloch W, Strüder HK, Hülsdünker T, Schenk A, ... 10. Berlin: Springer. pp. 251-7. doi:10.1038/sj.mp.4001613. ISBN 978-0-387-76760-4. . PMID 15583703. S2CID 21206951.. ; see the ...
The resulting deconstruction of cellular components is primarily carried out by specialized proteases known as caspases, but ... 279 (10): 8635-41. doi:10.1074/jbc.M310449200. PMID 14688254.. *^ Seemüller E, Lupas A, Stock D, Löwe J, Huber R, Baumeister W ... 10 (2): 104-115. doi:10.1038/nrm2630. PMID 19165213.. *^ Sakata E, Stengel F, Fukunaga K, Zhou M, Saeki Y, Förster F, ... 4 (10): 1335-7. doi:10.4161/cc.4.10.2107. PMID 16177571.. *^ Dharmasiri S, Estelle M (2002). "The role of regulated protein ...
HR has some similarities to animal pyroptosis, such as a requirement of caspase-1-like proteolytic activity of VPEγ, a cysteine ... November 2004). "VPEgamma exhibits a caspase-like activity that contributes to defense against pathogens". Current Biology. 14 ... When the cytoplasmic receptors MDA5 and RIG-I recognize a virus the conformation between the caspase-recruitment domain (CARD) ... 10 (6): 625-32. doi:10.1080/14653240802301872. PMID 18836917.. *^ Tonn T, Becker S, Esser R, Schwabe D, Seifried E (August 2001 ...
Nevertheless, TRADD binds FADD, which then recruits the cysteine protease caspase-8. A high concentration of caspase-8 induces ... On the other hand, activated caspases cleave several components of the NF-κB pathway, including RIP, IKK, and the subunits of ... 10 (1): 45-65. doi:10.1038/sj.cdd.4401189. PMID 12655295.. *^ Chen G, Goeddel DV (2002). "TNF-R1 signaling: a beautiful pathway ... 88 (10): 4220-4. Bibcode:1991PNAS...88.4220W. doi:10.1073/pnas.88.10.4220. PMC 51630. PMID 1709737.. ...
"Critical loss of CBP/p300 histone acetylase activity by caspase-6 during neurodegeneration". primary. The EMBO Journal. 22 (24 ... 10 (2): 99-106. doi:10.1080/17482960802320487. PMID 18688762.. *^ a b Piepers S, Veldink JH, de Jong SW, van der Tweel I, van ... 10 (2): 85-94. doi:10.1080/17482960802226148. PMID 18618304.. *^ a b Petri S, Kiaei M, Kipiani K, Chen J, Calingasan NY, Crow ... M (y) 10; H (ny) MC 13; D (y) 11 M (y) 30, 31; H (ny) ... 10]. Valproic scid. Valproic acid in mice studies restored ...
... to upregulate the activity of caspase-8. This causes cross talking of apoptotic signaling between caspase-8 and caspase-9 ... To remove the Cbz protecting group hydrogenolysis, under 50-60 psi of hydrogen with 10% Pd/C mixed with ethyl acetate and HCl, ... Upregulation of interferon gamma, IL-2 and IL-10 have been reported for pomalidomide and may contribute to its anti-angiogenic ... 10 (17): 5949-5956. doi:10.1158/1078-0432.CCR-04-0421. PMID 15355928. "Summary of product characteristics: Thalidomid Celgene ...
... condensed apoptotic nuclei and a 2-4 fold increase in cortical precursors that stained immunopositive for cleaved caspase-3.[27 ... 10 (6): 702-11. doi:10.1038/nn1903. PMID 17515902.. *^ Penzes P, Johnson RC, Sattler R, Zhang X, Huganir RL, Kambampati V, ... 107 (4): 306-10. doi:10.1002/ajmg.10133. PMID 11840487.. *^ Zimmer P, Mierau A, Bloch W, Strüder HK, Hülsdünker T, Schenk A, ... 10] It is also expressed in the retina, motor neurons, the kidneys, saliva, and the prostate.[11] ...
Caspase 9 can then go on to activate caspase 3 and caspase 7, which are responsible for destroying the cell from within. ... Caspase 3. Pro-apoptotic:. BAX. BAK1/Bcl-2 homologous antagonist killer. Bcl-2-associated death promoter. Anti-apoptotic:. Bcl- ... Apoptosis & Caspase 3 - PMAP The Proteolysis Map-animation. *UMich Orientation of Proteins in Membranes families/superfamily-78 ... This release of cytochrome c in turn activates caspase 9, a cysteine protease. ...
Angiotensinogen · Caspase · F12 · Kimotripsinogen · Pepsinogen · Proelastase · Prokarboksipolipeptidase · Prolipase · ... 3 HSD · .4 · .5 · .6 · .7 · .8 · .9 · .10 · .11 · .12 · .13 · .14 · .15 · .16 · .17 · .18 · .19 · .20 · .21 · .22 · .23 · .24 · ... 1 PA · .2 · .3 · .4 · .5 · .6 · .7 · .8 · .9 · .10 · .11 · .12 · .13 · .14 · .15 · .16 · .17 · .18 · .19 · .20 · .21 · .22 α- ... 1 Rsk · .2 PDK · .3 · .4 · .5 · .6 · .7 · .8 · .9 · .10 · .11 · .12 · .13 · .14 · .15 · .16 · DAPK · .18 · .19 · .20 · .21 · . ...
This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2 ... and may be instrumental in the execution of apoptosis following caspase activation. However p21 may inhibit apoptosis and does ... 10 (4): e0121396. doi:10.1371/journal.pone.0121396. PMC 4398562. PMID 25875127. AAAS EurekAlert! Press Release and NAE Podcast ... 10] This protein is encoded by the CDKN1A gene located on chromosome 6 (6p21.2) in humans.[11] ...
"Crocetin prevents retinal degeneration induced by oxidative and endoplasmic reticulum stresses via inhibition of caspase ... 2E,4E,6E,8E,10E,12E,14E)-2,6,11,15-Tetramethylhexadeca-2,4,6,8,10,12,14-heptaenedioic acid[2] ... InChI=1S/C20H24O4/c1-15(11-7-13-17(3)19(21)22)9-5-6-10-16(2)12-8-14-18(4)20(23)24/h5-14H,1-4H3,(H,21,22)(H,23,24)/b6-5+,11-7+, ... InChI=1/C20H24O4/c1-15(11-7-13-17(3)19(21)22)9-5-6-10-16(2)12-8-14-18(4)20(23)24/h5-14H,1-4H3,(H,21,22)(H,23,24)/b6-5+,11-7+,12 ...
"A novel form of DAP5 protein accumulates in apoptotic cells as a result of caspase cleavage and internal ribosome entry site- ... This article on a gene on human chromosome 10 is a stub. You can help Wikipedia by expanding it.. *v ... "Entrez Gene: EIF3S10 eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa".. ...
Non obstante, a TRADD únese a FADD, o cal despois recruta a cisteína protease caspase-8. Unha alta concentración de caspase-8 ... Ten varias acción sobre diversos órganos, xeralmente en conxunción coa IL-1 e a IL-6: *Sobre o hipotálamo: *Estimulación do ... A forma segregada do TNFα humano ten forma triangular de pirámide, e pesa arredor de 17 kD. Tanto a forma segregada coma a ... O TNFα pode producirse ectopicamente no marco dun tumor maligno e ten efectos similares aos da hormona paratiroide tanto en ...
Excess progenitor cell proliferation that leads to gross brain abnormalities is often lethal, as seen in caspase-3 or caspase-9 ... Kuida, K (1998). "Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9". Cell. 94: 325-337 ... to cells (such as feedback from neighbors, stress or DNA damage), mitochondria release caspase activators that trigger the cell ... Kroemer G, Martin SJ (2005). "Caspase-independent cell death". Nature Medicine. 11 (7): 725-30. doi:10.1038/nm1263. PMID ...
It is an energy dependent process mediated by proteolytic enzymes called caspases, which trigger cell death through the ... 77 (1): 3-10. doi:10.1093/bja/77.1.3. PMID 8703628.. *^ Klaassen, C.D., Ed.: Casarett and Doull's Toxicology: The Basic Science ... This page was last edited on 22 August 2019, at 10:00 (UTC). ...
Executioner Caspases (Caspase 3, Caspase 6 and Caspase 7). Once initiator caspases are activated, they produce a chain reaction ... Caspase-1, Caspase-4, Caspase-5 and Caspase-11 are considered 'Inflammatory Caspases'.[7] ... Caspase-1, Caspase-4 and Caspase-5 in humans, and Caspase-1 and Caspase-11 in mice play important roles in inducing cell death ... or direct activation of Executioner Caspases (Caspase 3, Caspase 6 and Caspase 7) to degrade cellular components as shown in ...
A interferencia de ARN ten un importante papel na defensa das células contra xenes parasitos, como virus e transposóns, pero ... "Caspase 8 small interfering RNA prevents acute liver failure in mice". Proc Natl Acad Sci USA 100 (13): 7797-802. PMC 164667 ... Estes ARN procesados son incorporados no complexo silenciador inducido por ARN (RISC), que ten como diana o ARN mensaxeiro para ... Os efectos fóra do obxectivo orixínanse cando un ARN introducido ten unha secuencia de bases que pode aparearse ...
This complex then cleaves procaspase-9, activating caspase-9 and eventually inducing apoptosis via caspase-3 activation. Hsp70 ... 22 (10): 1099-107. doi:10.14670/HH-22.1099. PMID 17616937.. *^ Sherman M, Multhoff G (October 2007). "Heat shock proteins in ... 32 (25): 3101-10. doi:10.1038/onc.2012.314. PMID 22824801.. *^ Lüders J, Demand J, Höhfeld J (February 2000). "The ubiquitin- ... It is also known to be phosphorylated[10] which regulates several of its functions.[11][12][13] ...
Ubiquitin ligases transfer ubiquitin to its pendant, proteins, and caspases, which engage in proteolysis in the apoptotic cycle ... 117 (6): 1003-10. PMID 3298579.. *^ Heitmann P (January 1968). "A model for sulfhydryl groups in proteins. Hydrophobic ... Degussa introduced a route from substituted thiazolines.[10] Following this technology, L-cysteine is produced by the ... 952-10-3056-9 Interaction of alcohol and smoking in the pathogenesis of upper digestive tract cancers - possible ...
Stegh AH, Barnhart BC, Volkland J, Algeciras-Schimnich A, Ke N, Reed JC, Peter ME (Feb 2002). "Inactivation of caspase-8 on ... Retinoid X receptor alpha.[10]. Ligands[edit]. A number of ligands for FXR are known, of both natural and synthetic origin.[11] ...
Caspase. *Caspase 1. *Caspase 2. *Caspase 3. *Caspase 4. *Caspase 5. *Caspase 6 ...
Also, it is extensively used in research for the detection of DNA damage,[34][35] caspase cleavage and apoptosis.[36] In ... The current record for a commercial instrument is ten lasers[6] and 30 fluorescence detectors.[7] Increasing the number of ... Apoptosis (quantification, measurement of DNA degradation, mitochondrial membrane potential, permeability changes, caspase ... 10: 145. doi:10.1186/1471-2105-10-145 - via BioMed Central.. *^ "FLow analysis with Automated Multivariate Estimation (FLAME)" ...
"Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9". Cell. 94 (3): 325-37. doi:10.1016/ ... Instead, the primary cortical gyri form first (beginning as early as gestational week 10 in humans), followed by secondary and ... Budday, Silvia; Raybaud, Charles; Kuhl, Ellen (2014-07-10). "A mechanical model predicts morphological abnormalities in the ... 10] Many theories since have been loosely tied to this hypothesis. ...
"N-APP binds DR6 to cause axon pruning and neuron death via distinct caspases". Nature 457 (7232): 981-989. doi:10.1038/ ... Retrieved on 10 Agosti 2010. *↑ Roan S. "Tapping into an accurate diagnosis of Alzheimer's disease", Los Angeles Times, 9 ... 33.0 33.1 Wenk GL (2003). "Neuropathologic changes in Alzheimer's disease". J Clin Psychiatry 64 Suppl 9: 7-10. PMID 12934968 . ... 10 Downing Street. Iliwekwa mnamo 2008-08-18. *↑ Mi padre no reconoció al Rey pero notó el cariño. El País (2008). Iliwekwa ...
"Newcastle disease virus exerts oncolysis by both intrinsic and extrinsic caspase-dependent pathways of cell death". Journal of ... doi:10.1128/JVI.01537-10. PMC 3126310. PMID 21471241.. *^ a b Mullen, JT; Tanabe, KK (2002). "Viral oncolysis" (PDF). The ... Mesogenic strains cause coughing, affect egg quality and production, and result in up to 10% mortality. Lentogenic strains ... 10][11] It appears the virus preferentially targets and replicates in certain types of tumor cells, leaving normal cells almost ...
Diaz F, Bourguignon LY (2000). «Selective down-regulation of IP(3)receptor subtypes by caspases and calpain during TNF alpha - ... 6 (10): 869-77. PMID 17827064. doi:10.1016/S1474-4422(07)70222-3. !CS1 manut: Uso explícito de et al. (link) !CS1 manut: Nomes ...
Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive ... This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene ... Caspase 8, Fas receptor, RYBP, TNFRSF1A and TNFRSF10B. The Proteolysis Map Caspase GRCh38: Ensembl release 89: ENSG00000003400 ... Caspase-10 is an enzyme that, in humans, is encoded by the CASP10 gene. This gene encodes a protein that is a member of the ...
Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-,-AMC and ... Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors ... PS01122 CASPASE_CYS, 1 hit. PS01121 CASPASE_HIS, 1 hit. PS50207 CASPASE_P10, 1 hit. PS50208 CASPASE_P20, 1 hit. PS50168 ... PS01122 CASPASE_CYS, 1 hit. PS01121 CASPASE_HIS, 1 hit. PS50207 CASPASE_P10, 1 hit. PS50208 CASPASE_P20, 1 hit. PS50168 ...
What is caspase-10? Meaning of caspase-10 medical term. What does caspase-10 mean? ... Looking for online definition of caspase-10 in the Medical Dictionary? caspase-10 explanation free. ... that activates the caspase-8 or caspase-10 initiator, and subsequently, the caspases effectors -3, -7 and possibly -6.. ... following the activation of caspase initiator caspase-10 and effector caspase-7.. Taiwanin A inhibits MCF-7 cancer cell ...
Mouse monoclonal Caspase-9 antibody [10-1-87] validated for WB and tested in Human. Immunogen corresponding to recombinant full ... Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically ... Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be ... All lanes : Anti-Caspase-9 antibody [10-1-87] (ab115792) at 1 µg/ml. Lane 1 : Jurkat cell lysate. Lane 2 : Jurkat cell lysate, ...
Compare caspase recruitment domain family member 10 ELISA Kits from leading suppliers on Biocompare. View specifications, ... caspase recruitment domain family member 10 ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established ... Your search returned 13 caspase recruitment domain family member 10 ELISA ELISA Kit across 1 supplier. ...
BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, etc. BioVisions products are currently being sold in more than 60 countries worldwide.
... caspase recruitment domain family, member 10), Authors: Gamze Ayaz, Mesut Muyan. Published in: Atlas Genet Cytogenet Oncol ... The structural domains of CARD10 protein are shown in different color boxes. CARD, caspase-recruitment domain; PDZ, PSD95, DLGA ... Caspase recruitment domain family, member 10, CARD10 (also known as CARMA3 or Bimp1), is a member of the membrane-associated ... CARD10 (caspase recruitment domain family, member 10). Written. 2014-08. Gamze Ayaz, Mesut Muyan. ...
These inhibit caspase-10 and may affect a variety of other apoptosis and tumor related proteins. ... Caspase-3 Inhibitor I, Cell Permeable An inhibitor of caspase-3, caspase-6, caspase-7, caspase-8, and caspase-10 sc-3074 1 mg. ... Caspase-3 Inhibitor An inhibitor of caspase-3, caspase-6, caspase-7, and caspase-10 210344-95-9. sc-3075 0.5 mg. $100.00 30 ... An inhibitor of caspase-3, caspase-6, caspase-7, and caspase-10 210344-95-9. sc-311558 sc-311558A 1 mg. 5 mg. $228.00 $970.00 ...
Anti-Caspase 10 pAb (GTX30487) is tested in Human samples. 100% Ab-Assurance. ... Caspase 10 antibody (caspase 10, apoptosis-related cysteine peptidase) for WB. ... Caspase10, 137215, MCH4, CASP10, FLICE2, Q92851, 843. Specificity. Reacts with the N terminus of all isoforms of human Caspase- ... Caspases are death effector domain-containing cysteine aspartases presumed to be at or near the apex of apoptotic signaling ...
... caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases ... This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene ... CASP10 / Caspase 10. caspase 10, apoptosis-related cysteine peptidase. CASP10 / Caspase 10 is a protein which is a member of ... CASP10, ALPS2, Caspase-10, Caspase 10, CASP-10, FADD-like ICE2, FLICE2, MCH4, ICE-like apoptotic protease 4, Apoptotic protease ...
... is used for detecting the activity of caspases that recognize the sequence AEVD using colorimetric method. (KA0730) - Products ... Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive ... This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene ... Caspase-10 Assay Kit (Colorimetric) is used for detecting the activity of caspases that recognize the sequence AEVD using ...
Research tested and proven Caspase-10/b-Flice 2 antibody. Antibody is guaranteed to work in western blot applications and is ... frequently other caspases). Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4 ... caspase-3, -6, and -7).. Caspases are regulated by a variety of stimili, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of ... Caspase 10/b Immunoblotting of SDS-extracts from 2 x 105 human Jurkat cells. Extracts were electrophoresed on 20% gels and ...
Caspase-2 Inhibitor Z-VDVAD-FMK (10 mM). Recommend to use at up to 1:5000 dilutions for cell culture ...
Caspase-Family Inhibitor Boc-D-FMK (10 mM). Recommend to use at 1:1000 - 1:5000 dilutions for cell culture ...
Caspases are cysteine proteases that mediate programmed cell death in phylogenetically diverse multicellular organisms. We ... Caspases are cysteine proteases that mediate programmed cell death in phylogenetically diverse multicellular organisms. We ... Inherited human Caspase 10 mutations underlie defective lymphocyte and dendritic cell apoptosis in autoimmune ... These encode amino acid substitutions that decrease caspase activity and interfere with death receptor-induced apoptosis, ...
Genetic polymorphisms of caspase 8 and 10 genes and risk of non hodgkin s lymphoma for Mona Hazem Elnagdy Saleh ... Genetic polymorphisms of caspase 8 and 10 genes and risk of non hodgkin s lymphoma for Mona Hazem Elnagdy Saleh * ... Genetic polymorphisms of caspase 8 and 10 genes and risk of non hodgkin s lymphoma for Mona Hazem Elnagdy Saleh. Author(S). ... Genetic polymorphisms of caspase 8 and 10 genes and risk of non hodgkin s lymphoma for Mona Hazem Elnagdy Saleh ...
... coli Caspase-10, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His• ... More,, Caspase-10, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His• ... Caspase-10, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag® ... Recombinant, human caspase-10 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single ...
Caspase-10 Inhibitor Drug Screening Kit (Fluorometric) can be used for research. ... Purified Caspase-10 Inhibitor Drug Screening Kit (Fluorometric) from Creative Biomart. ... 2X Reaction Buffer; Caspase Substrate AEVD-AFC (1 mM); DTT (1 M); Active Caspase-10 (Lyophilized); Caspase Inhibitor, Z-VAD-FMK ... Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts. The Caspase-10 Inhibitor ...
These nonapoptotic caspase functions involve prodomain-mediated activation of NF-kB. Processed caspases (caspase-8/10) encoding ... Roles of caspase-8 and caspase-10 in innate immune responses to double-stranded RNA Takahashi, K, Kawai, T, Kumar, H, Sato, S, ... Caspase-8 and caspase-10 activate NF-kappaB through RIP, NIK and IKKalpha kinases Shikama, Y, Yamada, M, Miyashita, T ... Caspases leave the beaten track: caspase-mediated activation of NF-kappaB Lamkanfi, M, Declercq, W, Vanden Berghe, T, ...
Flunarizine-induced DNA fragmentation was inhibited by the caspase-3 inhibitor z-DEVD-fmk, the caspase-8/caspase-10 inhibitor z ... but was not reduced in caspase-8-deficient Jurkat cells, indicating the involvement of caspase-10 upstream of caspase-3 ... Treatment of Jurkat cells with flunarizine resulted in caspase-3 activation, poly (ADP-ribose) polymerase cleavage, and ... and caspase-9 activation, although none of these events were necessary for apoptosis induction. Collectively, these findings ...
Caspase-10 Substrate AEVD-AFC; Appearance Liquid \ 1113-200 for more molecular products just contact us ... 16186808] Unique and overlapping substrate specificities of caspase-8 and caspase-10.. [15209560] Activation of caspases-8 and ... 9823333] Identification and characterization of murine caspase-14, a new member of the caspase family.. ... Ready-to-use fluorometric substrate for AEVD-dependent caspases. Activity of AEVDdependent caspase activity can be quantified ...
... biovis-caspase-10-inhibitor-aevd , Biovis \ Caspase-10 Inhibitor AEVD-FMK \ 1112-20C for more molecular products just contact ... Caspase-8 subunit p10]. [csp-1 Y48E1B.13] Caspase A (EC 3.4.22.36) [Cleaved into: Caspase A subunit p16; Caspase A subunit p14] ... Caspase-3 subunit p12]. [CASP3] Caspase-3 (CASP-3) (EC 3.4.22.56) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12] ... Caspase-14 subunit p17, mature form; Caspase-14 subunit p10, mature form; Caspase-14 subunit p20, intermediate form; Caspase-14 ...
... biovis-caspase-10-b-human , Biovis \ Caspase_10_b, human recombinant \ 1090B-25 for more molecular products just contact us ... Caspase-10b (also known as FLICE 2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase ... Caspase-14 subunit p17, mature form; Caspase-14 subunit p10, mature form; Caspase-14 subunit p20, intermediate form; Caspase-14 ... Caspase-14 subunit p17, mature form; Caspase-14 subunit p10, mature form; Caspase-14 subunit p20, intermediate form; Caspase-14 ...
Caspase-10 Inhibitor AEVD-FMK ; Appearance Liquid \ 1112-20C for more molecular products just contact us ... 15301743] Diosgenin induces apoptosis in HeLa cells via activation of caspase pathway.. [15276646] Involvement of caspase-10 in ... 1112-20C Caspase-10 Inhibitor AEVD-FMK ; Appearance Liquid Ask technical file . ... We recommend using 1000X dilutions for inhibiting caspase-10 and related caspase activity (e.g., Add 1 μl to 1 ml of culture ...
Executioner Caspases (Caspase 3, Caspase 6 and Caspase 7). Once initiator caspases are activated, they produce a chain reaction ... Caspase-1, Caspase-4, Caspase-5 and Caspase-11 are considered Inflammatory Caspases.[7] ... Caspase-1, Caspase-4 and Caspase-5 in humans, and Caspase-1 and Caspase-11 in mice play important roles in inducing cell death ... or direct activation of Executioner Caspases (Caspase 3, Caspase 6 and Caspase 7) to degrade cellular components as shown in ...
"ウサギ・ポリクローナル抗体 ab27525 交差種: Hu 適用: IHC-P…Caspase-10抗体一覧…画像、プロトコール、文献などWeb上の情報 ... Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-. -AMC and ... Synthetic peptide within Human Caspase-10 aa 450 to the C-terminus (N terminal). The exact sequence is proprietary.. Database ... Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and
Caspase-10/CASP-10抗体一覧…画像、プロトコール、文献などWeb上の情報 ... All lanes : Anti-Caspase-10/CASP-10 antibody (ab2012) at 1/1000 dilution. Lane 1 : HeLa whole cell lysate. Lane 2 : Jurkat ... Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-. -AMC and ... Green KA et al. Caspase-mediated Cleavage of Insulin Receptor Substrate. J Biol Chem 279:25149-56 (2004). PubMed: 15069074 ...
Caspase 10, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... Indoledione caspase activator, cell-permeable, Promotes apoptosome formation and activates caspase-9/caspase-3 pathway. ... Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive ... Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive ...
Initiator caspases: responsible for the activation of effector caspases. These caspases are activated through oligomerization ... Caspase-8 and caspase-10[edit]. [11] Caspases are cysteine proteases responsible for dismantling off the cell during apoptosis ... The pseudo-caspase domain of FLIPL is able to induce the conformational change in procaspase-8s caspase domain that is ... c-FLIPL (long): it consists of not only the tandem DEDs, but also a protease-like domain (homologous to caspase-8) in which ...
Caspase-10 in Mouse or Not?. By Reiner U. Jänicke, Dennis Sohn, Gudrun Totzke, Klaus Schulze-Osthoff ...
  • Caspase-10 is an enzyme that, in humans, is encoded by the CASP10 gene. (wikipedia.org)
  • CASP10 is an initiator-type caspase which is activated by, and interacts with, upstream adaptor molecules through CARD and DED protein-protein interaction domains. (thefreedictionary.com)
  • CASP10 / Caspase 10 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. (lsbio.com)
  • CASP10 (Caspase 10) is a Protein Coding gene. (genecards.org)
  • 3 reported cases ALPS-CASP10: Caspase 10. (wikipedia.org)
  • Autoimmune lymphoproliferative syndrome due to somatic FAS mutation (ALPS-sFAS) combined with a germline caspase-10 (CASP10) variation. (medscape.com)
  • Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. (neuromics.com)
  • They are expressed as latent zymogens and are activated by an autoproteolytic mechanism or by processing by other proteases (frequently other caspases). (neuromics.com)
  • Caspases are cysteine proteases that mediate programmed cell death in phylogenetically diverse multicellular organisms. (nih.gov)
  • Caspase-10b (also known as FLICE 2) is a member of the caspase-family of cysteine proteases. (antibody-antibodies.com)
  • Caspases (cysteinyl aspartate proteases) are involved in the signaling pathways of apoptosis, necrosis and inflammation. (genecards.org)
  • [2] The DED domain is found in inactive procaspases ( cysteine proteases ) and proteins that regulate caspase activation in the apoptosis cascade such as FAS-associating death domain-containing protein ( FADD ). (rug.nl)
  • Caspases, a family of cysteine proteases, play a central role in apoptosis. (jci.org)
  • The caspase family of cysteine proteases play a key role in apoptosis. (abcam.com)
  • Caspase 3 is synthesized as an inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. (abcam.com)
  • Recent studies suggest that this inhibition is achieved, at least in part, by S-nitrosylation of the active-site cysteine of caspases, a family of cysteine proteases that execute the death program ( 2-4 ). (sciencemag.org)
  • In mammals, a family of cysteine proteases (designated caspases) related to the Caenorhabditis elegans CED-3 protein appears to represent a major effector arm of the apoptotic program ( 5 ). (pnas.org)
  • The mammalian cell death proteases have been divided into proximal and distal caspases based on the their sites of action in the proteolytic caspase cascade ( 6 ). (pnas.org)
  • Mammalian IAP-1, -2, and XIAP directly bind and inhibit enzymatically active death proteases, caspase-3, and -7, but not the upstream protease caspase-8 ( 18 , 19 ). (pnas.org)
  • We examined the temporal profile of apoptosis after fluid percussion-induced traumatic brain injury (TBI) in rats and investigated the potential pathophysiological role of caspase-3-like proteases in this process. (jneurosci.org)
  • Together, these results implicate caspase-3-like proteases in neuronal apoptosis induced by TBI and suggest that the blockade of such caspases can reduce post-traumatic apoptosis and associated neurological dysfunction. (jneurosci.org)
  • Apoptosis is ultimately carried out by the sequential activation of initiator and executioner caspases, which constitute a family of intracellular proteases involved in dismantling the cell in an ordered fashion. (mdpi.com)
  • Caspases are the proteases responsible for dismantling the cell in an ordered and histologically distinct process termed apoptosis [ 1 ]. (mdpi.com)
  • Active caspases-8 and caspase-10, in turn, cleave and activate downstream effector proteases, including caspases-3 and caspase-7, causing apoptosis. (aacrjournals.org)
  • In response to diverse stimuli, mitochondria release a variety of cell death-inducing proteins into the cytosol, including cytochrome c , which activates caspase-9, and then cleaves and activates downstream effector proteases ( 9 ). (aacrjournals.org)
  • Caspases are a family of proteases that act as common death effector molecules in various forms of cell death ( Logue and Martin, 2008 ). (frontiersin.org)
  • Apoptosis requires the action of various classes of proteases, including a family of cysteine proteases known collectively as the caspases. (ahajournals.org)
  • Caspases, a family of cysteine proteases, are the central regulators of apoptosis. (cellsignal.com)
  • It should be noted that although IL-1 β and IL-18 processing is catalyzed most efficiently by caspase-1, other proteases can process IL-1 β under particular circumstances, like during high neutrophilic inflammation [ 4 - 6 ]. (hindawi.com)
  • Caspase-10 Polyclonal Antibody detects endogenous levels of Caspase-10 protein. (celltechgen.com)
  • T cells from patient and normal control supported in culture for >10 days with mitogen stimulation and IL-2 expansion and then exposed to anti-Fas IgM monoclonal antibody ALPS patient T cells: Do not die with anti-Fas monoclonal antibody exposure. (wikipedia.org)
  • Caspase-3 was efficiently immunoprecipitated with its specific antibody, but not with control antibody ( Fig. 1 B). Silver stains revealed that associated proteins did not significantly contaminate the caspase immunoprecipitates. (sciencemag.org)
  • Protein immunoblots of whole-cell lysates derived from 5 × 10 5 BJAB, 10C9 or Jurkat cells were done with an iNOS-specific antibody. (sciencemag.org)
  • Proteins were immunoprecipitated from 10C9 cell lysates using a caspase-3-specific monoclonal antibody (Caspase-3 IP), or with equal concentrations of an isotype-matched control antibody (Control IP). (sciencemag.org)
  • Caspase-3 levels in the immunoprecipitates were visualized by protein immunoblot using a caspase-3-specific antibody ( 5 ). (sciencemag.org)
  • Immunohistochemical analysis of frozen H1650 xenograft section, using Cleaved Caspase-3 (Asp175) Antibody #9661 . (cellsignal.com)
  • Company 1 cleaved caspase-3 rabbit polyclonal antibody shows high background and does not detect distinct bands in the expected 19/17 kDa range. (cellsignal.com)
  • Company 2 active caspase-3 rabbit polyclonal antibody detects multiple non-specific bands at various molecular weights but weakly detects the bands of interest in the 19/17 kDa and 12 kDa range. (cellsignal.com)
  • Company 1 active caspase-3 rabbit monoclonal antibody detects multiple non specific bands at various molecular weights. (cellsignal.com)
  • The antibody recognizes only the 17 kDa band corresponding to one form of the large fragment of cleaved caspase-3. (cellsignal.com)
  • In the extrinsic way, the apoptotic signal begins by the union of extra cellular ligands to the surface of cell receptors, resulting in a recruitment of the cytosolic adapter of proteins, that activates the caspase-8 or caspase-10 initiator, and subsequently, the caspases effectors -3, -7 and possibly -6. (thefreedictionary.com)
  • Caspase recruitment domain family, member 10, CARD10 (also known as CARMA3 or Bimp1), is a member of the membrane-associated guanylate kinase (MAGUK) superfamily proteins that act to organize signaling at the plasma membrane. (atlasgeneticsoncology.org)
  • This family of proteins contains Src-homology 3 (SH3), PDZ, GuK domains and caspase recruitment domain (CARD). (atlasgeneticsoncology.org)
  • Caspase family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. (scbt.com)
  • Caspase-10 Inhibitors offered by Santa Cruz inhibit caspase-10 and, in some cases, other apoptosis and tumor related proteins. (scbt.com)
  • Caspase_10_b, human recombinant recombinant proteins Most stable storage is at - 81 C or lower but some lyophilised proteins can be stored at +4C. (antibody-antibodies.com)
  • Gentaur suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Caspase_10_b, human recombinant. (antibody-antibodies.com)
  • Tumour growth can occur by a combination of factors, including a mutation in a cell cycle gene which removes the restraints on cell growth, combined with mutations in apoptopic proteins such as Caspases that would respond by inducing cell death in abnormally growing cells. (wikipedia.org)
  • The presence of amino acids that determine the solubility and aggregation to DED allowed to identify DED's in different proteins, such as caspase-8 and MC159. (rug.nl)
  • Cell differentiation, caspase inhibition and macromolecular synthesis blockage, but not Bcl-2 or Bcl-XL proteins, protect SH-SY5Y cells from apoptosis triggered by two cdk inhibitory drugs. (udl.cat)
  • Furthermore, we show that caspase-8 does not compete with other tandem DED proteins such as cFLIP or caspase-10 in binding via FADD to the receptor as current models suggest. (datamed.org)
  • Activation of apical caspases, such as caspase-8, through cell death receptors or other apoptotic stimuli leads to activation of downstream caspases, precipitous cleavage of target proteins and execution of the apoptotic program ( 7 , 8 ). (pnas.org)
  • The prodomains of several apical caspases contain a protein module termed caspase recruitment domain (CARD) that is conserved in several apoptosis regulatory molecules, including Apaf-1, RAIDD, and cellular inhibitors of apoptosis proteins (IAPs) ( 12 ). (pnas.org)
  • The CARD has been proposed to play a regulatory role in apoptosis by allowing proteins such as Apaf-1 to associate with caspase-9 ( 13 ). (pnas.org)
  • Two viral proteins, baculovirus p35 and cowpox virus CrmA, inhibit apoptosis by directly targeting caspases ( 14 , 15 ). (pnas.org)
  • By cleaving critical proteins, caspases lead to the changes that characterize apoptosis both morphologically and biochemically, such as chromatin condensation, loss of cell adhesion, cell shrinkage, membrane blebbing, DNA fragmentation, and finally formation of apoptotic bodies, which stimulate their own engulfment by phagocytes. (mdpi.com)
  • In the extrinsic pathway, ligation of the death receptors, such as Fas/CD95, TNFR1, death receptor (DR)4, and DR5, triggers the recruitment of adaptor proteins that activate either caspase-8 or caspase-10. (aacrjournals.org)
  • We report the cloning and characterization of two novel proteins that are splice isoforms of the caspase-10 family. (wikigenes.org)
  • UO126 modulated the levels of several intracellular proteins including B-cell lymphoma protein 2 (Bcl-2), myeloid cell leukemia-1 (Mcl-1) and caspase 8 homolog FLICE-inhibitory protein (cFLIP), and induced G 2 M cell-cycle arrest or apoptosis. (bloodjournal.org)
  • 4 - 10 Inhibition of Ras/MEK/ERK signaling has been shown to induce cell-cycle arrest and cell death by interfering with several intracellular survival and cell-cycle proteins. (bloodjournal.org)
  • Spi-2 proteins like CrmA potently inhibit caspases-1, -4 and -5, which produce proinflammatory cytokines, and caspase-8, which facilitates cytotoxic lymphocyte-mediated target cell death. (portlandpress.com)
  • The retention of Spi-2 proteins' caspase-8 specificity during chordopoxvirus evolution, despite this function being readily lost through cleavage site mutagenesis, suggests that caspase-8 inhibition is crucial for poxviral pathogenesis and spread. (portlandpress.com)
  • Once activated, initiation caspases cleave and activate downstream effector caspases (including caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. (cellsignal.com)
  • Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. (abcam.com)
  • View detailed caspase-10 Inhibitor specifications, including caspase-10 Inhibitor CAS number, molecular weight, molecular formula and chemical structure, by clicking on the product name. (scbt.com)
  • Caspases are regulated by a variety of stimili, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of the inhibitor of apoptosis (IAP) family such as BIRC1/NAIP, BIRC2/cIAP-1, BIRC3/cIAP-2, BIRC4/XIAP, BIRC5/Survivin, and BIRC7/Livin. (neuromics.com)
  • The Caspase-10 Inhibitor Drug Screening Kit provides an effective means for screening caspase inhibitors using fluorometric methods. (creativebiomart.net)
  • A synthetic peptide inhibitor that inhibits caspase-10 and related caspase activity. (antibody-antibodies.com)
  • The cleaved caspase 3 assay utilizes the Caspase-3 inhibitor, DEVD-FMK, conjugated to FITC (FITC-DEVD-FMK) as a marker. (abcam.com)
  • Thus, ARC represents an inhibitor of apoptosis expressed in muscle that appears to selectively target caspases. (pnas.org)
  • Intracerebroventricular administration of z-DEVD-fmk-a specific tetrapeptide inhibitor of caspase-3-before and after injury markedly reduced post-traumatic apoptosis, as demonstrated by DNA electrophoresis and TUNEL staining, and significantly improved neurological recovery. (jneurosci.org)
  • Z-VAD-FMK is a cell-permeable, irreversible pan-caspase inhibitor. (adooq.com)
  • Z-VDVAD-FMK (caspase-2 inhibitor I) and Z-DEVD-FMK (caspase-3 inhibitor II) were purchased from Calbiochem. (ahajournals.org)
  • The final DMSO concentrations of caspase inhibitor treatment groups (10 μmol/L, 100 μmol/L, and combined 100 μmol/L) were 0.05%, 0.5%, and 1.0% (vol/vol), respectively. (ahajournals.org)
  • This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. (wikipedia.org)
  • It cleaves and activates caspase-3, -4, -6, -7, -8 and -9, is recruited to Fas- and TNFR-1 receptors in a FADD-dependent manner, and may participate in the granzyme B apoptotic pathways. (thefreedictionary.com)
  • Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. (abcam.com)
  • The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. (abcam.com)
  • Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active-site thiol. (sciencemag.org)
  • Stimulation of cell surface death receptors activates caspase-8, which targets a limited number of substrates including BAP31, an integral membrane protein of the endoplasmic reticulum (ER). (rupress.org)
  • Taken together, these results show that restoration of TFPI-2 activates both intrinsic and extrinsic caspase-mediated, proapoptotic signaling pathways and induces apoptosis in U-251 cells. (aacrjournals.org)
  • Conclusions -OxyHb activates caspase-2 and -3 in cultured brain microvessel endothelial cells. (ahajournals.org)
  • Caspase-10 triggers Bid cleavage and caspase cascade activation in FasL-induced apoptosis. (semanticscholar.org)
  • Activity of AEVDdependent caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate AEVD-AFC at Ex. (antibody-antibodies.com)
  • Activation involves dimerization and often oligomerisation of pro-caspases, followed by cleavage into a small subunit and large subunit. (wikipedia.org)
  • Cleavage of the procaspase at the specific Asp-X bonds leads to the formation of the mature caspase, which comprises the heterotetramer p20 2 -p10 2 , and the release of the prodomain. (jci.org)
  • Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. (abcam.com)
  • Here we used mutational analysis and protein microsequencing to demonstrate that a caspase target site, located in the linker region between the Rel and the IκB-like domain, is the site of signal-dependent cleavage. (pnas.org)
  • We report the actual cleavage site and direct interactions between Dredd and Relish, which together provide strong evidence that Relish endoproteolysis is indeed carried out by the caspase Dredd. (pnas.org)
  • These compounds induced apoptosis of prostate cancer cells as characterized by cleavage of caspase-3, caspase-7, caspase-8, caspase-9, caspase-10, BID, and poly(ADP)ribose polymerase and by dependence on caspase activity. (aacrjournals.org)
  • Here, we report that the p20 caspase cleavage fragment of BAP31 can direct pro-apoptotic signals between the ER and mitochondria. (rupress.org)
  • Therefore, caspase-8 cleavage of BAP31 at the ER stimulates Ca 2+ -dependent mitochondrial fission, enhancing the release of cyt.c in response to this initiator caspase. (rupress.org)
  • Our results demonstrate the importance of caspase-mediated RIPK1 cleavage during embryonic development and show that caspase cleavage of RIPK1 not only inhibits necroptosis but also maintains inflammatory homeostasis throughout life. (nature.com)
  • Fig. 1: Heterozygous mutations of the RIPK1 caspase-8 cleavage site cause autoinflammatory disease. (nature.com)
  • Fig. 2: Homozygous mutation of the RIPK1 caspase-8 cleavage site in mice causes early embryonic lethality. (nature.com)
  • 3) Z-VDVAD-FMK and Z-DEVD-FMK (100 μmol/L) attenuated OxyHb-induced cell detachment, reduced caspase-2 and -3 activities, abolished OxyHb-induced DNA ladders, and prevented OxyHb-induced cleavage of PARP. (ahajournals.org)
  • Activation of caspase-3 requires cleavage at Asp175 into the activated p17/p19 and p12 protein fragments. (cellsignal.com)
  • CST Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664 recognizes the activated large fragment after cleavage (seen as a 17 kDa and 19 kDa doublet on western blot). (cellsignal.com)
  • CST Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664 recognizes the activated large fragment after cleavage (seen as a 17 kDa and 19 kDa doublet). (cellsignal.com)
  • This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. (wikipedia.org)
  • A gene on chromosome 2q33-q34 that encodes a protein belonging to the cysteine-aspartic acid protease (caspase) family which, once activated by proteolytic processing, plays a central role in the execution-phase of cell apoptosis, as well as various stages of embryological development. (thefreedictionary.com)
  • CARD10 (Caspase Recruitment Domain Family Member 10) is a Protein Coding gene. (genecards.org)
  • Overall design: Caspase-10 and control shRNA expressing HeLa cells were analysed for gene expression in respect to CD95L stimulation by RNA extraction and hybridization on Affymetrix microarrays. (datamed.org)
  • This signal-induced endoproteolysis requires the activity of several gene products, including the IκB kinase complex and the caspase Dredd. (pnas.org)
  • Unexpectedly, a role for a caspase in Relish activation was indicated by the fact that mutants in Dredd , a Drosophila caspase gene, are deficient in Relish processing and antimicrobial peptide production ( 10 - 12 ). (pnas.org)
  • In cancer, therefore, one would anticipate caspases to be frequently rendered inactive, either by gene silencing or by somatic mutations. (mdpi.com)
  • The protease family of the caspases, the mammalian homologues of the Caenorhabditis elegans death gene, is required for mammalian apoptosis. (ahajournals.org)
  • Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts. (creativebiomart.net)
  • Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-10 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors. (creativebiomart.net)
  • Inhibition of caspase-10 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors. (creativebiomart.net)
  • Note that in addition to apoptosis, Caspase-8 is also required for the inhibition of another form of programmed cell death called Necroptosis . (wikipedia.org)
  • In particular, it has been shown that NO synthase (NOS) activity can lead to caspase inhibition by a mechanism independent of cyclic guanosine monophosphate, that caspases can be S-nitrosylated by NO donors in cellular and in vitro systems, that the S-nitrosylation takes place solely on the active-site cysteine, and that this modification inhibits caspase activity in a reversible manner ( 2-4 ). (sciencemag.org)
  • Consistent with the inhibition of caspase-8, ARC attenuated apoptosis induced by FADD and TRADD and that triggered by stimulation of death receptors coupled to caspase-8, including CD95/Fas, tumor necrosis factor-R1, and TRAMP/DR3. (pnas.org)
  • All potently blocked caspases-1, -4, -5 and -8 activity but exhibited negligible inhibition of caspases-2, -3 and -6. (portlandpress.com)
  • We demonstrate that caspase-10 inhibits the activation of caspase-8 independent of cFLIP. (datamed.org)
  • Inhibits caspase processing and apoptosis induction in tumor cells in vitro (IC50 = 0.0015 - 5.8 mM). (adooq.com)
  • Catalytic activity of the caspase-8-FLIP L complex inhibits RIPK3-dependent necrosis. (nature.com)
  • Fas-AssociatedDeathDomain (FADD) and receptor interacting protein 1 (RIP1) are death domain containing molecules that interact with the C-terminal portion of IPS-1 and induce NF-kB through interaction and activation of initiator caspases (caspase-8 and -10). (reactome.org)
  • Executioner caspases have only rarely been found mutated or silenced, and also initiator caspases are only affected in particular types of cancer. (mdpi.com)
  • There is experimental evidence from transgenic mice that certain initiator caspases, such as caspase-8 and -2, might act as tumor suppressors. (mdpi.com)
  • Caspases are death effector domain-containing cysteine aspartases presumed to be at or near the apex of apoptotic signaling pathways. (genetex.com)
  • Among its related pathways are Direct p53 effectors and Apoptosis and survival Caspase cascade . (genecards.org)
  • However, it remains elusive whether propofol regulates caspase and/or mitogen-activated protein kinase (MAPK) pathways to induce apoptosis in Leydig tumor cells. (ncku.edu.tw)
  • In the present study, MA-10 mouse Leydig tumor cells were treated with propofol, and possible signal pathways associated with apoptosis were investigated. (ncku.edu.tw)
  • In conclusion, propofol may induce MA-10 cell apoptosis by activating caspase and MAPK pathways, and inhibiting the Akt pathway in MA-10 cells, demonstrating that propofol may be a potential anticancer agent against Leydig cell cancer. (ncku.edu.tw)
  • Third, pathways upstream of caspase activation might be disrupted in tumor cells. (mdpi.com)
  • Both pathways eventually fuel into a common effector phase that is characterized by the activation of caspases ( Fulda and Debatin, 2006 ). (frontiersin.org)
  • FADD recruits procaspase 8 and procaspase 10 into a death induced signaling complex (DISC). (rug.nl)
  • Caspase-8 and its regulator cFLIP control death signalling by binding to the receptor via DISC-bound FADD. (datamed.org)
  • Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. (wikipedia.org)
  • Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. (abcam.com)
  • During apoptosis procaspase-10 is processed at aspartate residues to form active caspase-10 which consists of two large (18 kDa) and two small (11 kDa) subunits in a heterotetramer form. (antibody-antibodies.com)
  • The processed form of Caspase 3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. (abcam.com)
  • The caspases are synthesized as inactive precursors that are proteolytically processed to generate active subunits. (pnas.org)
  • By utilizing caspase-8 knockout cells, we demonstrate that caspase-8 has to be placed upstream of both cFLIP and caspase-10 in the DISC. (datamed.org)
  • Both receptors recruit a Fas-associated DD adapter protein and activate several upstream caspases including caspase 8 and caspase 10, in addition to cleaving Bid with subsequent activation of caspase 9. (bloodjournal.org)
  • Depending on the structure of the prodomain and their function, caspases are typically divided into 3 major groups (Figure 1 A). The caspases with large prodomains are referred to as inflammatory caspases (group I) and initiator of apoptosis caspases (group II), while caspases with a short prodomain of 20-30 amino acids are named effector caspases (group III). (jci.org)
  • group III: apoptosis effector caspases. (jci.org)
  • The active caspase-10 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. (antibody-antibodies.com)
  • Active caspase10/b is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. (antibody-antibodies.com)
  • Subsequently, active caspases specifically process various substrates that are implicated in apoptosis and inflammation. (jci.org)
  • Many caspase substrates are cleaved during apoptosis and pertaining to their functional significance have yet to merge. (agscientific.com)
  • AG Scientific provides various selection of caspase substrates and be able to ship FedEx directly to your lab, from small-scale to bulk orders. (agscientific.com)
  • In this scholarly study, we examined Rabbit Polyclonal to Caspase 10 p53 and P-gp appearance, aswell as clinical variables in sufferers with DLBC. (lefiestus16.com)
  • 1068-20, -80), DTT (1201-1) and other reagents used for caspase assays are also available separately. (antibody-antibodies.com)
  • Review the full set of caspase assays , or the apoptosis assay and apoptosis marker guide . (abcam.com)
  • Caspase-9 and caspase-3 activity assays showed increased activity, indicating enhanced apoptosis. (aacrjournals.org)
  • Recombinant full length protein corresponding to Human Caspase-9. (abcam.com)
  • Caspase-10, Human, Recombinant is expressed in E. coli as a single polypeptide. (merckmillipore.com)
  • Please read carefully the data sheet of the Caspase_10_b, human recombinant. (antibody-antibodies.com)
  • Caspase_10_b, human recombinant Human samples 80 % of the research is conducted on human samples. (antibody-antibodies.com)
  • One unit of the recombinant caspase-10/b is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT. (antibody-antibodies.com)
  • The recombinant active human caspase-10/b was expressed in E. coli. (antibody-antibodies.com)
  • Processed caspases (caspase-8/10) encoding the DED (death effector domain) strongly activate NF-kB. (reactome.org)
  • As far as it is known, the homotypic interactions that activate caspase and trigger apoptosis are mediated by asymmetrical surface contacts between partners (like DED1 and DED2 in the caspase-8 case). (rug.nl)
  • Additionally, propofol diminished the phosphorylation of Akt to activate apoptosis in MA-10 cells. (ncku.edu.tw)
  • Presumably, postmitotic cell death results from a failure of mitosis associated with tetraploidy, which is essential to activate a cell death program in G 1 to prevent further polyploidization ( 10 ). (aacrjournals.org)
  • 15 Different death stimuli may activate different caspases. (ahajournals.org)
  • Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. (wikipedia.org)
  • Fas and Fas-ligand interact to trigger the caspase cascade, leading to cell apoptosis. (wikipedia.org)
  • Additionally, Annexin V/propidium iodide double staining further confirmed that propofol could induce MA-10 cell apoptosis. (ncku.edu.tw)
  • We report here two kindreds with autoimmune lymphoproliferative syndrome (ALPS) type II, characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects, that harbor independent missense mutations in Caspase 10. (nih.gov)
  • Effective means for screening caspase inhibitors using fluorometric methods. (creativebiomart.net)
  • Ready-to-use fluorometric substrate for AEVD-dependent caspases. (antibody-antibodies.com)
  • For a complete caspase assay protocol, please refer to BioVision's Caspase Fluorometric or Colorimetric Assay Kits. (antibody-antibodies.com)
  • Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. (creativebiomart.net)
  • Caspase-10/c mRNA is specifically up-regulated upon TNF stimulation, suggesting a potential role of this isoform in amplifying the apoptotic response to extracellular stimuli such as cytokines. (wikigenes.org)
  • This Review gives an overview of caspases and their classification, structure, and substrate specificity. (jci.org)
  • We compared the caspase specificity of CrmA to three orthologs from orthopoxviruses and four from more distant chordopoxviruses. (portlandpress.com)
  • 10:15 Acetylating Polymyxin Antibiotics: Clues toward Substrate Specificity of PA3944 Gcn5-Related N-Acetyltransferase of Unknown Function. (asbmb.org)
  • The large and small subunit associate with each other to form an active heterodimer caspase. (wikipedia.org)
  • Caspases, a family of c ysteinyl a spartate- s pecific p rote ases , are synthesized as zymogens with a prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). (jci.org)
  • We recommend using 1000X dilutions for inhibiting caspase-10 and related caspase activity (e.g. (antibody-antibodies.com)
  • Synthetic peptide: ISAQTPRPPMRRWSSVS, corresponding to amino acids 505 -521 of Human Caspase 10. (genetex.com)
  • Caspase 10/b Immunoblotting of SDS-extracts from 2 x 105 human Jurkat cells. (neuromics.com)
  • Synthetic peptide within Human Caspase-10 aa 450 to the C-terminus (N terminal). (abcam.co.jp)
  • Structure of caspase-1 (CASP1), originally called interleukin-1 beta-converting enzyme (ICE), the first human caspase to be identified. (wikipedia.org)
  • Synthetic peptide corresponding to Human Caspase-10/CASP-10 aa 505-521 (C terminal). (abcam.co.jp)
  • The tertiary structure of the domain has been described from the crystallization of caspase 8 in the human. (rug.nl)
  • Synthesized peptide derived from the Internal region of human Caspase-10. (celltechgen.com)
  • Caspase-3 zymogens were found to be S-nitrosylated on their catalytic-site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway. (sciencemag.org)
  • To address these issues, we immunoprecipitated caspase-3 from three different human B and T cell lines that express NOS ( Fig. 1 A) ( 5 ). (sciencemag.org)
  • NOS and caspase-3 expression in human lymphocyte cell lines. (sciencemag.org)
  • Likewise, Apaf-1, a human protein that resembles C. elegans CED-4, interacts with caspase-9, a step that is required for the activation of the downstream protease caspase-3 ( 11 ). (pnas.org)
  • Real-time quantitative live-cell imaging and lactate dehydrogenase assay showed that cisplatin stimulated caspase 3/7 activation and cytotoxicity in a human proximal tubule epithelial cell line which were attenuated by inhibitors of the FasL/Fas system and poly [ADP-ribose] polymerase-1. (nih.gov)
  • Caspase recruitment domain family member 12 (CARD12, CLAN) is a caspase-associated recruitment domain (CARD)-containing protein and is expressed at varying degrees in normal human tissues. (allelebiotech.com)
  • Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. (gentaur.com)
  • The balance of lymphocytes and neutrophils in adult animals is quite different: human blood is neutrophil rich (50-70% neutrophils, 30-50% lymphocytes) whereas mouse blood has a strong preponderance of lymphocytes (75-90% lymphocytes, 10-25% neutrophils) ( 10 ). (jimmunol.org)
  • Western blot analysis of extracts from C6 (rat), NIH/3T3 (mouse), and Jurkat (human) cells, untreated or treated with staurosporine #9953 (1uM, 3 hrs) or etoposide #2200 (25uM, 5hrs) as indicated, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664 . (cellsignal.com)
  • These encode amino acid substitutions that decrease caspase activity and interfere with death receptor-induced apoptosis, particularly that stimulated by Fas ligand and TRAIL. (nih.gov)
  • To explore the mechanism(s) involved in caspase-8 activation, we examined cell surface expression of death receptor (DR)4 and DR5 after triterpenoid treatment. (aacrjournals.org)
  • Western blot analysis showed increased transcriptional activities of Fas ligand, tumor necrosis factor-α, Bax, Fas-associated death domain, and tumor necrosis factor receptor 1-associated death domain as well as elevated levels of cleaved caspases and poly(ADP-ribose) polymerase. (aacrjournals.org)
  • In the extrinsic pathway, the ligation of death receptors by their ligands including tumor necrosis factor (TNF) receptor, CD95 (APO-1/Fas), or TNF-related apoptosis-inducing ligand (TRAIL) receptors, initiates the formation of a multimeric protein complex called the death-inducing signaling complex (DISC) that drives activation of caspase-8 ( Ashkenazi, 2008b ). (frontiersin.org)
  • Inflammasomes, of which different types have been identified, are multiprotein complexes containing pattern recognition receptors belonging to the Nod-like receptor family or the PYHIN family and the protease caspase-1. (hindawi.com)
  • FasL expression was up-regulated by taiwanin A at both the transcriptional and translational levels, following the activation of caspase initiator caspase-10 and effector caspase-7. (thefreedictionary.com)
  • drugs blocking the activation of Caspase-1 have been used to improve the health of patients. (wikipedia.org)
  • Activation of caspase-1 in the context of the inflammasome is responsible for the proteolytic processing of the immature forms of Interleukin-1 β (IL-1 β ) and IL-18, two very powerful proinflammatory cytokines with pleiotropic activities [ 3 ]. (hindawi.com)
  • Similar to other caspases, caspase-10 also exists in cells as an inactive proenzyme. (antibody-antibodies.com)
  • Caspases are synthesised as inactive zymogens (pro-caspases) that are only activated following an appropriate stimulus. (wikipedia.org)
  • Existing in inactive forms within the cell, caspases are cleaved to become active enzymes upon the start of apoptosis. (agscientific.com)
  • These data seem to question a general tumor-suppressive role of caspases. (mdpi.com)
  • We discuss several possible ways how tumor cells might evade the need for alterations of caspase genes. (mdpi.com)
  • First, alternative splicing in tumor cells might generate caspase variants that counteract apoptosis. (mdpi.com)
  • Second, in tumor cells caspases might be kept in check by cellular caspase inhibitors such as c-FLIP or XIAP. (mdpi.com)
  • Finally, caspase-independent cell death mechanisms might abrogate the selection pressure for caspase inactivation during tumor development. (mdpi.com)
  • Herein, apoptosis and/or non-apoptotic functions of caspases may even promote tumor development. (mdpi.com)
  • We thus propose a model wherein caspases are preserved in tumor cells due to their functional contributions to development and progression of tumors. (mdpi.com)
  • Your search returned 13 caspase recruitment domain family member 10 ELISA ELISA Kit across 1 supplier. (biocompare.com)
  • Involved in the activation cascade of caspases responsible for apoptosis execution. (uniprot.org)
  • We further show that DISC formation and/or stability depends on caspase-8 but is independent from its enzymatic activity. (datamed.org)
  • Further analysis showed that the enzymatic activity of caspase-8 was inhibited by ARC in 293T cells. (pnas.org)
  • Increased caspase-3-like, but not caspase-1-like, enzymatic activity was found in cytosolic extracts from injured cortex. (jneurosci.org)
  • Caspase-10 Assay Kit (Colorimetric) is used for detecting the activity of caspases that recognize the sequence AEVD using colorimetric method. (abnova.com)
  • In some contexts, caspase-8 can bypass mitochondria and directly cleave downstream caspases. (rupress.org)
  • Caspases are usually involved in apoptosis and inflammation but they also exhibit nonapoptotic functions. (reactome.org)
  • By elucidating the function of Caspase-10, a close homologue of caspase-8, we unexpectedly found that caspase-10 negatively regulates caspase-8-mediated cell death signalling in the DISC. (datamed.org)
  • Identification of a New Caspase Homologue: Caspase-14," Cell Death and Differentiation 5: 838-846, 1998. (patentgenius.com)
  • Caspases ( c ysteine- asp artic prote ases , c ysteine asp art ases or c ysteine-dependent asp artate-directed prote ases ) are a family of protease enzymes playing essential roles in programmed cell death (including apoptosis , pyroptosis and necroptosis ) and inflammation . (wikipedia.org)
  • A ) The caspase family. (jci.org)
  • Caspase 3 (also known as CPP32, YAMA and apopain) is the most extensively studied apoptotic protein among caspase family members. (abcam.com)
  • Alternatively, caspase-8 indirectly transfers the signal to apoptosis via mediators, for example Bid, a proapoptotic, BH3-only domain containing protein of the Bcl-2 family ( Adams and Cory, 2007 ). (frontiersin.org)
  • Identification and Characterization of Murine Caspase-14, a New Member of the Caspase Family," Cancer Research 58: 5201-5205, 1998. (patentgenius.com)
  • 11:10 Elucidating the Molecular Basis of Protein and Polymer Display in Pathogenic Bacteria for Novel Anti- virulence Drug Development. (asbmb.org)
  • Moreover, paclitaxel has been shown to induce apoptosis in G 1 and S stages, but induce both apoptosis and necrosis in G 2 -M phase ( 10 ). (aacrjournals.org)
  • In this study, we determined whether TFPI-2 restoration could induce apoptosis through the caspase-mediated signaling pathway. (aacrjournals.org)
  • 4. Incubate cells on ice for 10 minutes. (antibody-antibodies.com)
  • Additionally, scientists have used caspases as cancer therapy to kill unwanted cells in tumours. (wikipedia.org)
  • Cleaved Caspase-3 Staining Kit (FITC) ab65613 provides a convenient means for sensitive detection of activated Caspase-3 in living cells. (abcam.com)
  • FITC-DEVD-FMK is cell permeable, non-toxic, and irreversibly binds to activated Caspase-3 in apoptotic cells. (abcam.com)
  • The FITC label allows detection of activated / cleaved caspase-3 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader. (abcam.com)
  • Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. (abcam.com)
  • In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). (abcam.com)
  • Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (e.g. mantle zone B cells). (abcam.com)
  • Caspase-3 (Casp-3 IP) and isotype-matched control IgG2a immunoprecipitates (Control IP) (200 μl) from Jurkat cells were analyzed on a silver-stained polyacrylamide gel. (sciencemag.org)
  • Furthermore, cleaved caspase-8, -9 and -3, and/or poly(ADP-ribose) polymerase were significantly activated following treatment of propofol in MA-10 cells. (ncku.edu.tw)
  • Coenzyme Q10 (CoQ 10 ) acts by scavenging reactive oxygen species for protecting neuronal cells against oxidative stress in neurodegenerative diseases. (springer.com)
  • In addition, the CoQ 10 significantly blocked activation of astroglial and microglial cells in ischemic retina. (springer.com)
  • This study was undertaken to investigate the effect of 2 caspase inhibitors, Z-VDVAD-FMK and Z-DEVD-FMK, in the protection of endothelial cells from OxyHb-induced apoptosis. (ahajournals.org)
  • Methods -Cultured bovine brain microvascular endothelial cells (passages 5 to 9) were exposed to OxyHb (10 μmol/L) for 24 to 72 hours with and without caspase inhibitors. (ahajournals.org)
  • 2) OxyHb increased caspase-2 and -3 activities, produced DNA ladders, and cleaved PARP in endothelial cells. (ahajournals.org)
  • 9 10 11 12 We previously reported that OxyHb induced apoptosis in cultured endothelial cells in a time- and concentration-dependent manner. (ahajournals.org)
  • In this study we investigated the effect of OxyHb on the activities of caspase-2 and -3 and the prophylaxis effect of caspase inhibitors on OxyHb-induced apoptosis in bovine brain microvessel endothelial cells. (ahajournals.org)
  • During treatments with caspase inhibitors and OxyHb, the cells were incubated in CS-C Medium (Cell Systems Corporation) without growth factor and with 1% (vol/vol) fetal bovine serum. (ahajournals.org)
  • Control cells received 1.0% DMSO in the cell density study and DNA ladder and 0.5% DMSO in the caspase activity study and Western blot study. (ahajournals.org)
  • Cell-permeable and irreversible peptide inhibitors are also available for different caspases. (neuromics.com)
  • There are other identified roles of caspases such as cell proliferation, tumour suppression, cell differentiation, neural development and axon guidance and ageing. (wikipedia.org)