Acidic phospholipids composed of two molecules of phosphatidic acid covalently linked to a molecule of glycerol. They occur primarily in mitochondrial inner membranes and in bacterial plasma membranes. They are the main antigenic components of the Wassermann-type antigen that is used in nontreponemal SYPHILIS SERODIAGNOSIS.
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
Derivatives of phosphatidic acid in which the hydrophobic regions are composed of two fatty acids and a polar alcohol is joined to the C-3 position of glycerol through a phosphodiester bond. They are named according to their polar head groups, such as phosphatidylcholine and phosphatidylethanolamine.
A class of nucleotide translocases found abundantly in mitochondria that function as integral components of the inner mitochondrial membrane. They facilitate the exchange of ADP and ATP between the cytosol and the mitochondria, thereby linking the subcellular compartments of ATP production to those of ATP utilization.
Rare congenital X-linked disorder of lipid metabolism. Barth syndrome is transmitted in an X-linked recessive pattern. The syndrome is characterized by muscular weakness, growth retardation, DILATED CARDIOMYOPATHY, variable NEUTROPENIA, 3-methylglutaconic aciduria (type II) and decreases in mitochondrial CARDIOLIPIN level. Other biochemical and morphological mitochondrial abnormalities also exist.
Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)
Electron transfer through the cytochrome system liberating free energy which is transformed into high-energy phosphate bonds.
Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
A contagious venereal disease caused by the spirochete TREPONEMA PALLIDUM.
A class of enzymes that transfers substituted phosphate groups. EC 2.7.8.
The two lipoprotein layers in the MITOCHONDRION. The outer membrane encloses the entire mitochondrion and contains channels with TRANSPORT PROTEINS to move molecules and ions in and out of the organelle. The inner membrane folds into cristae and contains many ENZYMES important to cell METABOLISM and energy production (MITOCHONDRIAL ATP SYNTHASE).
The voltage difference, normally maintained at approximately -180mV, across the INNER MITOCHONDRIAL MEMBRANE, by a net movement of positive charge across the membrane. It is a major component of the PROTON MOTIVE FORCE in MITOCHONDRIA used to drive the synthesis of ATP.
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
A major protein fraction of milk obtained from the WHEY.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a choline moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and choline and 2 moles of fatty acids.
Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.

Nitric-oxide-induced apoptosis in human leukemic lines requires mitochondrial lipid degradation and cytochrome C release. (1/1010)

We have previously shown that nitric oxide (NO) stimulates apoptosis in different human neoplastic lymphoid cell lines through activation of caspases not only via CD95/CD95L interaction, but also independently of such death receptors. Here we investigated mitochondria-dependent mechanisms of NO-induced apoptosis in Jurkat leukemic cells. NO donor glycerol trinitrate (at the concentration, which induces apoptotic cell death) caused (1) a significant decrease in the concentration of cardiolipin, a major mitochondrial lipid; (2) a downregulation in respiratory chain complex activities; (3) a release of the mitochondrial protein cytochrome c into the cytosol; and (4) an activation of caspase-9 and caspase-3. These changes were accompanied by an increase in the number of cells with low mitochondrial transmembrane potential and with a high level of reactive oxygen species production. Higher resistance of the CD95-resistant Jurkat subclone (APO-R) cells to NO-mediated apoptosis correlated with the absence of cytochrome c release and with less alterations in other mitochondrial parameters. An inhibitor of lipid peroxidation, trolox, significantly suppressed NO-mediated apoptosis in APO-S Jurkat cells, whereas bongkrekic acid (BA), which blocks mitochondrial permeability transition, provided only a moderate antiapoptotic effect. Transfection of Jurkat cells with bcl-2 led to a complete block of apoptosis due to the prevention of changes in mitochondrial functions. We suggest that the mitochondrial damage (in particular, cardiolipin degradation and cytochrome c release) induced by NO in human leukemia cells plays a crucial role in the subsequent activation of caspase and apoptosis.  (+info)

Enzymatic properties of vesicle-reconstituted human cytochrome P450SCC (CYP11A1) differences in functioning of the mitochondrial electron-transfer chain using human and bovine adrenodoxin and activation by cardiolipin. (2/1010)

The recently reported heterologous expression and purification of both human cytochrome P450SCC and adrenodoxin [Woods, S.T., Sadleir, J., Downs, T., Triantopoulos, T., Haedlam, M.J. & Tuckey, R.C. (1998) Arch. Biochem. Biophys. 353, 109-115] has enabled us to perform studies with the membrane-reconstituted human enzymes to better understand the side-chain cleavage reaction in humans. Human P450SCC was successfully reconstituted into dioleoylphosphatidylcholine vesicles with and without cardiolipin and its enzymatic properties characterized in the membrane-bound state. Enhancement of the P450SCC activity and significant activation by cardiolipin were observed when human adrenodoxin instead of bovine adrenodoxin was used as electron donor. In the absence of cardiolipin, Km for cholesterol was decreased twice in the case of human adrenodoxin indicating enhanced cholesterol binding. On the other hand, in the presence of cardiolipin in the membrane both Km and V for cholesterol were decreased with human adrenodoxin as electron donor. Kinetic analysis of the interaction between human P450SCC and its redox partners provided evidence for enhanced binding of the human electron donor to human P450SCC indicated by both an increased V and decreased Kd for human adrenodoxin compared with the values with bovine adrenodoxin. Because no similar effects were observed in Tween 20 micelles, these results suggest that the phospholipid membrane may play an important role in the interaction of human adrenodoxin with human P450SCC.  (+info)

Phospholipid composition of Rickettsia prowazeki grown in chicken embryo yolk sacs. (3/1010)

The phospholipid composition and phospholipid fatty acid composition of purified Rickettsia prowazeki were determined. The lipid phosphorous content was 6.8 +/- 1.3 microgram/mg of total rickettsial protein. The major phospholipid was phosphatidylethanolamine (60 to 70%); phosphatidylglycerol constituted 20%, and phosphatidylcholine constituted 15%. Small amounts of phosphatidylserine and cardiolipin were detected. The principal fatty acids were 18:1, 16:1, and 16:0. The fatty acid composition of the phosphatidylcholine in the rickettsial extracts was very different than that of the other rickettsial phosphatides and very similar to that of normal yolk sac phosphatidylcholine. The specific of the phosphatidylcholine of rickettsiae grown in the presence of 32P was markedly lower than that of phosphatidylethanolamine and phosphatidylglycerol. It is suggested that the phosphatidylcholine in the rickettsial extract is yolk sac derived and either tightly absorbed or exchanged into the rickettsial membrane.  (+info)

Conformationally altered beta 2-glycoprotein I is the antigen for anti-cardiolipin autoantibodies. (4/1010)

Anti-cardiolipin autoantibodies (aCL) induce thrombosis and recurrent fetal death. These antibodies require a 'cofactor', identified as beta 2-glycoprotein I (beta 2-GPI), to bind phospholipids. We show here that aCL can bind beta 2-GPI in the absence of phospholipid. Binding of aCL to beta 2-GPI is dependent upon the beta 2-GPI being immobilized on an appropriate surface including cardiolipin, irradiated polystyrene and nitrocellulose membrane. This effect cannot be explained by increased antigen density of beta 2-GPI immobilized on these surfaces. Rather, conformational changes that occur following the interaction of beta 2-GPI with phospholipid render this protein antigenic to aCL. Liquid-phase beta 2-GPI was not antigenic for aCL. Thus, aCL cannot bind circulating beta 2-GPI. These findings may explain why patients with aCL can remain healthy for many years but then undergo episodes of thrombosis or fetal loss without changes in their circulating aCL profile, as the triggering event for these pathologies can be predicted to be one that renders beta 2-GPI antigenic for aCL.  (+info)

Transbilayer movement and distribution of spin-labelled phospholipids in the inner mitochondrial membrane. (5/1010)

The transmembrane diffusion and equilibrium distribution of spin-labelled phosphatidylethanolamine (PE*), phosphatidylcholine (PC*) and cardiolipin (CL*) were investigated in purified mitochondrial inner membranes using electron spin resonance spectroscopy. Using the back exchange technique, we found that the outside-inside movement of PE* and PC* in beef-heart inner mitochondrial membranes was rapid (t1/2 in the range 10-15 min at 30 degrees C). The steady-state distributions in non-energised mitoplasts were approximately 30% in the inner leaflet for PC* and 39% for PE*. Within the limits of probe concentration that can possibly be used in these experiments, the initial velocity of the inward movement was not saturable with respect to the amount of analogue added to the membranes, suggesting that the spin-labelled phospholipids diffused passively between the two leaflets of the inner mitochondrial membrane. In energised mitoplasts, PC* behaviour was not affected, PE* diffused approximately two times faster toward the inner monolayer but reached the same plateau. Treatment of energised mitochondria with N-ethylmaleimide did not affect PC* diffusion, while the kinetics of PE* internalisation became identical to that of PC*. Similar results were found when PC* and PE* movements were studied in mitoplasts from beef heart, rat liver or yeast. The spin-labelled cardiolipin, which possesses four long chains, had to be introduced in the mitoplast with some ethanol. After equilibration (t1/2 of the order of 13 min at 30 degrees C), the transmembrane distribution suggested that approximately half of the cardiolipin analogue remained in the outer leaflet. These results do not allow us to determine if a specific protein (or flippase) is involved in the phospholipid transmembrane traffic within inner mitochondrial membranes, but they show that lipids can rapidly flip through the mitochondrial membrane.  (+info)

Involvement of Arg-328, Arg-334 and Arg-342 of DnaA protein in the functional interaction with acidic phospholipids. (6/1010)

We reported previously that three basic amino acids (Arg-360, Arg-364 and Lys-372) of DnaA protein are essential for its functional interaction with cardiolipin. In this study, we examined the effect of mutation of some basic amino acids in a potential amphipathic helix (from Lys-327 to Ile-345) of DnaA protein on this interaction. ATP binding to the mutant DnaA protein, in which Arg-328, Arg-334 and Arg-342 were changed to acidic amino acids, was less inhibited by cardiolipin than that of the wild-type protein, as was the case for mutant DnaA protein with mutations of Arg-360, Arg-364 and Lys-372. A mutant DnaA protein with mutations of all six basic amino acids showed the most resistance to the inhibition of ATP binding by cardiolipin. These results suggest that Arg-328, Arg-334 and Arg-342, like Arg-360, Arg-364 and Lys-372, are also involved in the functional interaction between DnaA protein and acidic phospholipids.  (+info)

Maternal cardiolipin, beta 2-glycoprotein-I and prothrombin antibody expression in high-risk pregnancies with bilateral abnormal uterine artery Doppler waveforms. (7/1010)

OBJECTIVE: To compare the frequency of maternal serum antiphospholipid antibodies (to cardiolipin, beta 2-glycoprotein I and prothrombin) in pregnancies presenting with bilateral abnormal uterine artery Doppler waveforms. DESIGN: Retrospective analysis of stored serum. SUBJECTS: Cases comprised 47 singleton pregnancies with bilateral abnormal uterine artery Doppler waveforms at 24 weeks of gestation, followed from 20 weeks, and controls were 100 healthy pregnancies with normal uterine artery Doppler waveforms. METHODS: Ultrasound examination utilized a 5-MHz curvilinear transabdominal transducer with pulsed and color Doppler facilities. Antiphospholipid antibodies were analyzed by ELISA methodology, and reference ranges were established using the geometric mean +/- 2 SD of healthy non-pregnant adults. Human chorionic gonadotropin (hCG) levels were obtained from patient notes. RESULTS: Anticardiolipin antibodies were detected in 11 (23%) of the cases (IgG, n = 7; IgM, n = 6) compared with ten (10%) of the controls (p < 0.05). Low titer anticardiolipin IgG (range, 5.5-35.3; median, 6.3 GPL units) and anticardiolipin IgM (range, 3.4-14.7; median, 5.3 MPL units) were detected in cases. Amongst the cases, adverse perinatal outcomes were more common in the presence of raised levels of anticardiolipin antibodies. Anti-beta 2-glycoprotein I IgG was not detected in any of the cases. Antiprothrombin IgG was not detected, but antiprothrombin IgM occurred in 10.6% of cases compared with 2% of controls. CONCLUSIONS: Women with persistent bilateral abnormal uterine artery. Doppler waveforms in mid-gestation were more likely to express raised levels of anticardiolipin antibodies than healthy controls with normal uteroplacental perfusion. Anticardiolipin antibodies without anti-beta 2-glycoprotein I binding may be involved in the pathogenesis of uteroplacental ischemia in a proportion of high-risk pregnancies.  (+info)

Polar lipids of four Listeria species containing L-lysylcardiolipin, a novel lipid structure, and other unique phospholipids. (8/1010)

The membrane lipids of Listeria innocua, Listeria monocytogenes, Listeria seeligeri and Listeria welshimeri were fractionated on DEAE-cellulose and purified by chromatography on silica gel and/or preparative TLC. The lipid structures were elucidated by chemical and chromatographic means. The polar lipid composition of the four listeria species was similar. Phospholipids predominated. They consisted of phosphatidylglycerol, L-lysylphosphatidylglycerol, cardiolipin [bis(phosphatidyl)glycerol] and L-lysylcardiolipin. A phospholipid more polar than cardiolipin, possibly two L-lysyl derivatives of it, sn-glycero-1-phosphoglycolipid, its D-alanyl derivative, and polyprenol phosphate were also detected. Towards the end of exponential growth, the relative amounts of cardiolipin and L-lysylcardiolipin increased, approaching 47-78% lipid phosphorus with a ratio of L-lysylcardiolipin to cardiolipin of 0.25-1.6. As shown by fast atom bombardment-mass spectrometry, cardiolipin and L-lysylcardiolipin consisted of five molecular species due to various fatty acid combinations. L-lysylcardiolipin has so far not been found in nature. It belongs to the recently discovered class of substituted cardiolipins. Its occurrence in the four listeria species tested shows that it is a characteristic lipid component of the L. monocytogenes line of descent. Further studies on the lipid pattern of members of the other descent line are required to decide whether lysylcardiolipin can serve as a genus-specific chemotaxonomic marker for listeriae.  (+info)

Targeting mitochondrial cardiolipin and the cytochrome c/cardiolipin complex to promote electron transport and optimize mitochondrial ATP synthesis. Academic Article ...
CL(18:2(9Z,12Z)/18:1(9Z)/18:1(9Z)/16:0) is a cardiolipin (CL). Cardiolipins are sometimes called double phospholipids because they have four fatty acid tails, instead of the usual two. They are glycerophospholipids in which the O1 and O3 oxygen atoms of the central glycerol moiety are each linked to one 1,3-diacylglyerol chain. Their general formula is OC(COP(O)(=O)OC[[email protected]@H](CO[R1])O[R2])COP(O)(=O)OC[[email protected]@H](CO[R3])O[R4], where R1-R4 are four fatty acyl chains. CL(18:2(9Z,12Z)/18:1(9Z)/18:1(9Z)/16:0) contains one chain of (9Z,12Z-octadecadienoyl) at the C1 position, two chains of (9Z-octadecenoyl) at the C2 and C3 positions, one chain of hexadecanoic acid at the C4 position fatty acids. Cardiolipins are known to be present in all mammalian cells, especially cells with a high number of mitochondria. De novo synthesis of Cardiolipins begins with condensing phosphatidic acid (PA) with cytidine-5-triphosphate (CTP) to form cytidine-diphosphate-1,2-diacyl-sn-glycerol (CDP-DG). Glycerol-3-phosphate is ...
CL(i-12:0/i-22:0/i-22:0/i-21:0) is a cardiolipin (CL). Cardiolipins are sometimes called double phospholipids because they have four fatty acid tails, instead of the usual two. They are glycerophospholipids in which the O1 and O3 oxygen atoms of the central glycerol moiety are each linked to one 1,3-diacylglyerol chain. Their general formula is OC(COP(O)(=O)OC[[email protected]@H](CO[R1])O[R2])COP(O)(=O)OC[[email protected]@H](CO[R3])O[R4], where R1-R4 are four fatty acyl chains. CL(i-12:0/i-22:0/i-22:0/i-21:0) contains one chain of 10-methylundecanoic acid at the C1 position, two chains of 20-methylheneicosanoic acid at the C2 and C3 positions, one chain of 19-methyleicosanoic acid at the C4 position fatty acids. Cardiolipins are known to be present in all mammalian cells, especially cells with a high number of mitochondria. De novo synthesis of Cardiolipins begins with condensing phosphatidic acid (PA) with cytidine-5-triphosphate (CTP) to form cytidine-diphosphate-1,2-diacyl-sn-glycerol (CDP-DG). Glycerol-3-phosphate ...
TY - JOUR. T1 - Lysocardiolipin acyltransferase regulates TGF-β mediated lung fibroblast differentiation. AU - Huang, Long Shuang. AU - Jiang, Peiyue. AU - Feghali-Bostwick, Carol. AU - Reddy, Sekhar P.. AU - Garcia, Joe G.N.. AU - Natarajan, Viswanathan. PY - 2017/11. Y1 - 2017/11. N2 - Lysocardiolipin acyltransferase (LYCAT), a cardiolipin remodeling enzyme, plays a key role in mitochondrial function and vascular development. We previously reported that reduced LYCAT mRNA levels in peripheral blood mononuclear cells correlated with poor pulmonary function outcomes and decreased survival in IPF patients. Further LYCAT overexpression reduced lung fibrosis, and LYCAT knockdown accentuated experimental pulmonary fibrosis. NADPH Oxidase 4 (NOX4) expression and oxidative stress are known to contribute to lung fibroblast differentiation and progression of fibrosis. In this study, we investigated the role of LYCAT in TGF-β mediated differentiation of human lung fibroblasts to myofibroblasts, and ...
Cardiolipin is found predominantly in the membranes of mitochondria and bacteria. It is a unique diphosphatidylglycerol containing four acyl groups. In animal tissues 80% of the acyl groups are composed of linoleic acid (18:2(n-6)).The identification of Barth′s syndrome, a disease associated with abnormalities in the fatty acid composition of cardiolipin causing cardiomyopathy and neutropenia, has displayed the importance of cardiolipin in mitochondrial functionality. Cardiolipin effects mitochondrial bioenergetics by providing essential structural and functional support to proteins localized within the mitochondria. Specifically, interaction of cardiolipin with cytochrome oxidase and adenine nucleotide translocase is required in order for both of these mitochondrial enzymes to maintain activity.Cardiolipin has been shown to perform a role in a number of other specific biological activities, including oxidative phosphorylation, apoptosis, cholesterol translocation and gene
CL(14:0/14:0/18:1(11Z)/22:0) is a cardiolipin (CL). Cardiolipins are sometimes called a double phospholipid because they have four fatty acid tails, instead of the usual two. CL(14:0/14:0/18:1(11Z)/22:0) contains two chains of tetradecanoic acid at the C1 and C2 positions, one chain of (11Z-octadecenoyl) at the C3 position, one chain of docosanoic acid at the C4 position. While the theoretical charge of cardiolipins is -2, under normal physiological conditions (pH near 7), the molecule may carry only one negative charge. In prokaryotes such as E. coli, the enzyme known as diphosphatidylglycerol synthase catalyses the transfer of the phosphatidyl moiety of one phosphatidylglycerol to the free 3-hydroxyl group of another, with the elimination of one molecule of glycerol. In E. coli, which acylates its glycerophospholipids with acyl chains ranging in length from 12 to 18 carbons and possibly containing an unsaturation, or a cyclopropane group more than 100 possible CL molecular species are ...
Genetic analysis (chromosome/ Karyotype analysis): Blood samples should be taken from man and woman and tested for some probable abnormalities in the chromosomes, (missing chromosomes or additions to each chromosome, and translocations of chromosomes (in which all chromosomes are present but in different locations).. Anti Phospholipid Antibodies (APA) - cardiolipins are proteins found in many cells in your body. They help regulate blood flow throughout the body. However, when your body looks at the cardiolipins as an enemy or invader, it will attack them by creating antibodies . The three main groups anticardiolipins are: IgG, IgM, IgA.. Anti Cardiolipin Antibodies (ACA)- cells that attack the nuclei of other cells in your body. This is a mistake made by the body, thinking the good guys are the bad guys. Low levels should cause no problems.. Anti Nuclear Antibodies (ANA)- Again this is your body mistaking the good for the bad. Only this time it attacks the nuclei of the cell(s).. Lupus Anti ...
Acyl-CoA:lysocardiolipin acyltransferase. Possesses both lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities. Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors. Acts as a remodeling enzyme for cardiolipin, a major membrane polyglycerophospholipid. Converts lysophosphatidic acid (LPA) into phosphatidic acid (PA) with a relatively low activity. Required for establishment of the hematopoietic and endothelial lineages ...
Cardolipins are a separate class of phospholipids present in the cell membranes of organisms from all domains of life. In studying the enzyme that is responsible for producing cardiolipins in archaea biochemists discovered a single archaeal enzyme can produce a range of natural and non-natural cardiolipins, as well as other phospholipids.
All-trans-retinoic-acid (ATRA) is a promising agent in the prevention/treatment of breast-cancer. There is growing evidence that reprogramming of cellular lipid metabolism contributes to malignant transformation and progression. Lipid metabolism is implicated in cell differentiation and metastatic colonization and it is involved in the mechanisms of sensitivity/resistance to different anti-tumor agents. The role played by lipids in the anti-tumor activity of ATRA has never been studied. We used 16 breast cancer cell-lines whose degree of sensitivity to the anti-proliferative action of ATRA is known. We implemented a non-oriented mass-spectrometry based approach to define the lipidomic profiles of each cell-line grown under basal conditions and following treatment with ATRA. To complement the lipidomic data, untreated and retinoid treated cell-lines were also subjected to RNA-sequencing to define the perturbations afforded by ATRA on the whole-genome gene-expression profiles. The number and functional
Kristin Dittenhafer-Reed, PhD, Van Andel Institute Researcher in the MacKeigan Laboratory that focuses on identifying and understanding the genes and signaling pathways that, when mutated, contribute to the pathophysiology of cancer and neurodegeneration.. Altered cellular metabolism and the evasion of cell death are two hallmarks of cancer. Both of these hallmarks involve the mitochondria, the metabolic centers of the cell and critical players in initiating apoptosis. This seminar will highlight studies of a specific mitochondrial membrane component, cardiolipin, which may serve as a molecular bridge connecting altered metabolism and resistance to cell death. Cardiolipin is required for proper mitochondrial structure and function, and importantly, it facilitates the cellular events that initiate apoptosis. Cancer cells are able to limit or evade apoptosis, often allowing cells to resist chemotherapy and causing a significant clinical problem. Using cellular biology and quantitative biochemistry ...
The importance of the content of anionic phospholipids [cardiolipin (CL) and phosphatidylglycerol (PG)] in the osmotic adaptation and in the membrane structure of Bacillus subtilis cultures was investigated. Insertion mutations in the three putative cardiolipin synthase genes (ywiE, ywnE and ywjE) were obtained. Only the ywnE mutation resulted in a complete deficiency in cardiolipin and thus corresponds to a true clsA gene. The osmotolerance of a clsA mutant was impaired: although at NaCl concentrations lower than 1·2 M the growth curves were similar to those of its wild-type control, at 1·5 M NaCl (LBN medium) the lag period increased and the maximal optical density reached was lower. The membrane of the clsA mutant strain showed an increased PG content, at both exponential and stationary phase, but no trace of CL in either LB or LBN medium. As well as the deficiency in CL synthesis, the clsA mutant showed other differences in lipid and fatty acids content compared to the wild-type, suggesting a
1-MLCL(0:0/16:0/16:0/16:0) is a monolysocardiolipin (MLCL). Monolysocardiolipins have three fatty acid tails, instead of the usual two. 1-MLCL(0:0/16:0/16:0/16:0) contains one chain of at the C1 position, three chains of hexadecanoic acid at the C2, C3 and C4 positions. MLCL is present in eukaryotes as part of the metabolic cycle of mitochondrial lipids. Removal of one acyl chain from a cardiolipin results in generation of monolysocardiolipin (MLCL). MLCL has been used as an inter-mediate in the synthesis of spin-labeled CL to study the interaction of CL with mitochondrial enzymes. Because a role for MLCL has been suggested in apoptosis, this molecule has been used to study its interaction with various enzymes involved in lipid remodeling and apoptosis. There are two species of monolysocardiolipins, 1-MLCL which is missing a fatty acid in position R1 the and 2-MLCL which is missing a fatty acid in position R4 ...
The design of a human immunodeficiency virus-1 (HIV-1) immunogen that can induce broadly reactive neutralizing antibodies is a major goal of HIV-1 vaccine development. Although rare human monoclonal antibodies (mAbs) exist that broadly neutralize HIV-1, HIV-1 envelope immunogens do not induce these antibody specificities. Here we demonstrate that the two most broadly reactive HIV-1 envelope gp41 human mAbs, 2F5 and 4E10, are polyspecific autoantibodies reactive with the phospholipid cardiolipin. Thus, current HIV-1 vaccines may not induce these types of antibodies because of autoantigen mimicry of the conserved membrane-proximal epitopes of the virus. These results may have important implications for generating effective neutralizing antibody responses by using HIV-1 vaccines.. ...
Fig. 2. Assay of mAbs 2F5 and 4E10 against lipids and specificity of 2F5 and 4E10 mAb binding to cardiolipin. Microtiter plates were coated with 0.2 to 10 μg of lipid, blocked with 3% phosphate-buffered saline (PBS)-bovine serum albumin (BSA), and mAbs were diluted in PBS-Tween 20 (0.05%) containing 3% BSA and 2% normal goat serum. (A) Enzyme-linked immunosorbent assay (ELISA) reactivity of mAbs 4E10 (solid bars) and 2F5 (open bars) to CL, PS, PC, PE, and SM. Whereas both 4E10 and 2F5 reacted with cardiolipin, only 4E10 reacted with the other lipids tested. The reactivity of control human anti-CCR5 binding site mAb 1.7b was negative (33). Both mAbs 4E10 and 2F5 bound directly to cardiolipin, because cardiolipin binding in ELISA did not require the presence of bovine serum as a source of β2 glycoprotein-1; as an additional negative control, the reactivity of mAbs against empty methanol-coated plates was negative for 2F5 and was seen only at concentrations of 4E10≥2.5 μg per milliliter (33). ...
Chemical synthesis of diphosphatidyl glycerol, a long-chain fatty acid ester of diphosphatidyl glycerol, phosphatidyl diglyceride and phosphatidyl glycerophosphate has stimulated a structural comparison with natural cardiolipin. Although in certain properties the various polyglycerol phospholipids are quite similar, the results of enzymic hydrolyses with phospholipase A (EC 3.1.1.4), acylation studies, optical ... read more rotation measurements and chromatography of the intact phospholipids and deacylated products indicated that beef-heart cardiolipin has a diphosphatidyl glycerol structure. Conclusive evidence was obtained by means of the breakdown of the phospholipids with phospholipase C (EC 3.1.4.3). The enzyme was found to hydrolyse both natural and synthetic diphosphatidyl glycerol into 1,2-diglyceride and 1,3-glycerol diphosphate, phosphatidyl glycerophosphate being an intermediate hydrolysis product. show less ...
Define cardiolipin. cardiolipin synonyms, cardiolipin pronunciation, cardiolipin translation, English dictionary definition of cardiolipin. n. A phospholipid usually obtained from beef heart, used in combination with phosphatidylcholine and cholesterol as an antigen to diagnose syphilis
CLSAs vision is that partners will enrich the CLSA research platform, promote and foster its development into a dynamic, rich, comprehensive and unique resource for aging research. This premium resource will result in beneficial rewards for the partners through facilitating rapid adoption of sound research into health practice, programs, policies, services and products that will contribute to healthy aging and enhanced quality of life for older adults. The CLSA has been successful in additional fundraising with a variety of partners to support the implementation and baseline data collection phase of the CLSA (2010-2015). The CLSA continues to develop partnerships for the first (2015-2018) and second (2018-2021) follow-ups to support and sustain the long-term vision of the CLSA. A full list of CLSA partnerships is available on the Partners section of the CLSA website.. ...
Another focus of our research on cytochrome c is the proteins interaction with anionic cardiolipin containing liposomes by CD, absorption and resonance Raman spectroscopy. The specific aim of this project is to fully characterize structure and energy landscape of oxidized and reduced cytochrome c after the binding to the surface of such liposomes and after dissociation form the surface. These liposome serve as a model for the innermembrane of mitochondria. This will lead us to understand why the protein gains peroxidase activity on membrane surface if challenged with hydrogen peroxide. A detailed study aimed at exploring the binding and subsequent conformational changes of ferricytochrome c to cardiolipin containing liposomes have revealed two protein binding sites. For liposomes with 20% cardiolipin binding via site 1 is insensitive to the addition of NaCl and mostly preserves the native state, including the Fe-M80 linkage. Binding via site 2, however, causes a conformational transition into a ...
The Canadian Longitudinal Study on Aging (CLSA) is a large, national, long-term study of more than 50,000 individuals who were between the ages of 45 and 85 when recruited. These participants will be followed until 2033 or death. The aim of the CLSA is to find ways to help us live long and live well, and understand why some people age in healthy fashion while others do not.. Between 2010 and 2015, the CLSA recruited and collected information from more than 50,000 individuals. Now the study has entered its next phase where each individual recruited between 2010 and 2015 (baseline) will be re-contacted for the first follow-up. The first follow-up will be conducted between 2015 and 2018. During this phase, the CLSA research team will collect the same type of information that was collected at baseline, along with several new measures that have been introduced.. The CLSA is a longitudinal design, meaning that it will follow people over a long period of time. However, researchers will not wait years ...
The mitochondrial calcium uniporter is a Ca2+‐activated Ca2+ channel that is essential for dynamic modulation of mitochondrial function in response to cellular Ca2+ signals. It is regulated by two paralogous EF‐hand proteins-MICU1 and MICU2, but the mechanism is unknown. Here, we demonstrate that both MICU1 and MICU2 are stabilized by Ca2+. We reconstitute the MICU1-MICU2 heterodimer and demonstrate that it binds Ca2+ cooperatively with high affinity. We discover that both MICU1 and MICU2 exhibit affinity for the mitochondria‐specific lipid cardiolipin. We determine the minimum Ca2+ concentration required for disinhibition of the uniporter in permeabilized cells and report a close match with the Ca2+‐binding affinity of MICU1-MICU2. We conclude that cooperative, high‐affinity interaction of the MICU1-MICU2 complex with Ca2+ serves as an on-off switch, leading to a tightly controlled channel, capable of responding directly to cytosolic Ca2+ signals. ...
Mitochondrial lipids in neurodegeneration. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Ornithinyl ester of phosphatidyl glycerol was found to accumulate in Mycobacterium 607 under acidic conditions (pH 5.6) of growth or in cultures of ultraviolet-irradiated (320 to 420 nm) bacilli. There was a corresponding decrease in cardiolipin content of the organisms under these conditions. ...
Despite decades of research, there has been little new information on what biological mechanisms make bacterial pneumonia get worse, said senior author Rama K. Mallampalli, MD, a professor in the Acute Lung Injury Center of Excellence, University of Pittsburgh School of Medicine, and pulmonary division chief at the VA Pittsburgh Healthcare System.. Our study reveals some of the molecular steps that can lead to lung injury after infection and shows us new avenues for pneumonia therapy that dont have to target bacteria, as antibiotics do, Mallampalli says.. The researchers found that lung fluid from humans and mice with pneumonia contains abnormally high levels of cardiolipin, a structural molecule that is typically found in the membranes of energy-making mitochondria. A carrier protein called Atp8b1 transports the molecule from the lung fluid into the cell, acting as a pump that regulates cardiolipin levels.. Infection leads to the death of cells, and that releases cellular components, ...
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The Knowledge Translation and Communications Committee (KTCC) is the primary body responsible for initiating and facilitating knowledge translation and communications activities that support the objectives of the CLSA. It is a standing sub-committee of the CLSA Operations Committee, which provides recommendations to the CLSA Scientific Management Team.. The KTCC is composed of five voting members who are appointed by the CLSA Operations Committee (OC) for a term of two years.. The composition will include individuals, both internal and external to the CLSA, with knowledge translation expertise as well as representatives of key target audiences such as researchers, health-care providers and policy-makers. The KTCC will include at least one member of the OC.. The committee is supported by the CLSA communications officer who, as a non-voting member, will assist in coordinating the activities of the committee and maintaining documentation in collaboration with the chair. The committee will also ...
In vitro, the TRIAP1:PRELID3A complex mediates the transfer of phosphatidic acid (PA) between liposomes and probably functions as a PA transporter across the mitochondrion intermembrane space. Phosphatidic acid import is required for cardiolipin (CL) synthesis in the mitochondrial inner membrane.
Cardiolipin (CL) is a structurally unique dimeric phospholipid localized in the inner mitochondrial membrane where it is required for optimal mitochondrial function. In addition to its role in maintaining membrane potential and architecture, CL is known to provide essential structural and functional …
Manufacture and distribution of animal blood products, plasma, serum, microbiological diagnostic kits and quality control products, Buckinghamshire, UK
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
Tafazzin is a protein that in humans is encoded by the TAZ gene. Tafazzin is highly expressed in cardiac and skeletal muscle. It is involved in the metabolism of cardiolipin. Tafazzin functions as a phospholipid-lysophospholipid transacylase. The mutation of the tafazzin gene is associated with a number of clinical disorders including Barth syndrome (BTHS) (type II 3-Methylglutaconic aciduria), dilated cardiomyopathy (DCM), hypertrophic DCM, endocardial fibroelastosis, and left ventricular noncompaction (LVNC). Tafazzin is responsible for remodeling of a phospholipid cardiolipin (CL), the signature lipid of the mitochondrial inner membrane. As a result, BTHS patients exhibit defects in CL metabolism, including aberrant CL fatty acyl composition, accumulation of monolysocardiolipin (MLCL) and reduced total CL levels. The protein was identified by Italian scientists Silvia Bione et al. in 1996. Owing to the complex procedure required for the identification of tafazzin, the protein was named after ...
Quantitative and qualitative alterations of mitochondrial cardiolipin have been implicated in the pathogenesis of Barth syndrome, an X-linked cardioskeletal myopathy caused by a deficiency in tafazzin, an enzyme in the cardiolipin remodeling pathway. A tafazzin-deficient Drosophila model of Barth syndrome that is characterized by low cardiolipin (CL) concentration, abnormal cardiolipin fatty acyl composition, abnormal mitochondria, and poor motor function has been generated earlier. This study shows that tafazzin deficiency in Drosophila disrupts the final stage of spermatogenesis, spermatid individualization, and causes male sterility. This phenotype can be genetically suppressed by inactivation of the gene encoding a calcium-independent phospholipase A2, iPLA2-VIA, which also prevents cardiolipin depletion/monolysocardiolipin accumulation, although in wild-type flies inactivation of the iPLA2-VIA does not affect the molecular composition of cardiolipin. Furthermore, it was shown that treatment ...
Barth syndrome (BTHS) is an X-linked genetic condition, usually transmitted from mother to son (although there is a relatively high incidence of new mutations in Barth syndrome and one confirmed case report of a female Barth syndrome patient). A mother who is a carrier of a Barth syndrome mutation (the gene is named tafazzin - also called TAZ or G4.5) shows no signs or symptoms of this disorder herself, probably due to skewed X-chromosome inactivation.. There is a 50% chance that a boy born to a female carrier will have Barth syndrome, whereas girls born to a carrier have a 50% risk of being carriers themselves. All daughters of a male with Barth syndrome will be carriers, however no sons will be affected. Because there are proven non-carrier mothers, all mothers of Barth syndrome children should be tested in order to define the genetic risk in each family.. Any male child related through the female line to a Barth syndrome individual should be tested for the disorder, as there can be great ...
Lipids are ubiquitous and serve numerous biological functions; thus lipids have been shown to have great potential as candidates for elucidating biomarkers and pathway perturbations associated with disease. Methods expanding coverage of the lipidome increase the likelihood of biomarker discovery and could lead to more comprehensive understanding of disease etiology. We introduce LipidMatch, an R-based tool for lipid identification for liquid chromatography tandem mass spectrometry workflows. LipidMatch currently has over 250,000 lipid species spanning 56 lipid types contained in in silico fragmentation libraries. Unique fragmentation libraries, compared to other open source software, include oxidized lipids, bile acids, sphingosines, and previously uncharacterized adducts, including ammoniated cardiolipins. LipidMatch uses rule-based identification. For each lipid type, the user can select which fragments must be observed for identification. Rule-based identification allows for correct annotation of
Anticardiolipin antibodies purified from serum from patients with systemic lupus erythematosus (SLE) by cardiolipin micelles were studied for their effects on lymphocytes and neutrophils. At a concentration of 160 micrograms/ml they markedly suppressed the [3H]thymidine incorporation of mononuclear cells stimulated by phytohaemagglutinin (4.9 (SEM 1.9%) of the control) and pokeweed mitogen (26.7 (10.5%) of the control). In addition, anticardiolipin antibodies changed the cell cycle of phytohaemagglutinin stimulated lymphocytes such that the S and G2+M phases were significantly diminished (G0/G1 = 64.62%, S = 20.59%, G2+M = 14.78% in the presence of normal human IgG v G0/G1 = 86.07%, S = 10.32%, G2+M = 3.59% in the presence of anticardiolipin antibodies). The suppression of lymphocyte proliferation by anticardiolipin antibodies was shown not to be caused by an alteration of T cell subpopulations. However, the interleukin 2 receptors on the cell surface and the soluble interleukin 2 receptors in ...
The results of this study show that the synthesis of the inner mitochondrial membrane phospholipid, cardiolipin, is markedly inhibited following infection of human embryonic lung or hamster embryo fibroblast cells with herpes simplex virus type 1 or 2. The synthesis of other phospholipids, i.e., phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, and phosphatidylinositol, is relatively unaffected.
Tafazzin knockdown causes hypertrophy of neonatal cardiac myocytes (34), and mutation of the tafazzin gene causes dilated cardiomyopathy in Barth syndrome (64). Our work with neonatal cardiac fibroblasts (NVFs) showed that tafazzin knockdown increased ROS production, activated MAPKs including p42/44 and p38, stimulated transcriptional and translational factors, which in turn activated cell cycle regulators, and increased DNA and protein synthesis. On the other hand, tafazzin knockdown also decreased intracellular ATP, activated AMPK, and halted the energy-consuming process (i.e., cell proliferation), ultimately resulting in multinucleation, hypertrophy, and enhanced collagen secretion.. Tafazzin plays an important role in de novo cardiolipin synthesis and remodeling in the mitochondria. Tafazzin knockdown leads to reduced cardiolipin, which is consistent with previous studies involving yeast (28), Drosophila (72), and human skin fibroblasts (68). To be consistent with what is reported in mice ...
General Information. There are several laboratory tests that can be undertaken to confirm the diagnosis of Barth syndrome. Finding a tafazzin gene (TAZ, also called G4.5) mutation that clearly disables protein synthesis or function is the most definitive test for Xq28-linked Barth syndrome. In addition, the finding of a severely depressed level of tetralinoleoyl-cardiolipin [Schlame et al (2002)], [Vreken et al (2000)] in platelets, cultured cells, or muscle tissue appears to be specific for Barth syndrome caused by mutations in the tafazzin gene. However, because completion of these tests can take more time than desirable when a child has critical cardiac disease, other laboratory tests, as discussed below, in the appropriate clinical setting can be all but diagnostic of Barth syndrome. Although not all clinicians follow the regulations, it is important to recognize that diagnostic information that is given to a patient or patients family must be provided by a laboratory that has met that ...
Hydrothermal experiments were conducted at ca. 1 to 7000 bars and 700 to 1250 °C in 121 rhyolitic to basaltic systems to determine Cl solubility in silicate melts, i.e., the maximum Cl concentration in melts that are saturated in a hydrosaline liquid with or without an aqueous or aqueous-carbonic vapor. The Cl concentration of melts increases with the Cl contents of the fluid unless the melt coexists with vapor plus hydrosaline liquid at fixed pressure and temperature; this phase assemblage buffers the Cl content of each phase with increasing Cl in the system. The Cl content of fluid(s)-saturated melts is independent of the CO2 concentration of the saline liquid ± vapor with up to 21 wt% CO2 in the fluid(s). The experiments show that Cl dissolution in aluminosilicate melts increases with temperature and pressure. Chlorine solubility is also a function of melt composition; it increases with the molar ([Al1/2+Ca1/2+Mg1/2+Na]/Si) of the melt.. These experimental data have been integrated with ...
Catalyzes the synthesis of cardiolipin (CL) (diphosphatidylglycerol) by specifically transferring a phosphatidyl group from CDP-diacylglycerol to phosphatidylglycerol (PG). CL is a key phospholipid in mitochondrial membranes and plays important roles in maintaining the functional integrity and dynamics of mitochondria under both optimal and stress conditions ...
Translocation of ions across biological membranes is an essential process of life. It is enabled by membrane proteins and relies on tight integration of these proteins into the lipid bilayer. The tight seal is the prerequisite for the generation of electrochemical proton gradients which are the key to biological energy conversion. It is now generally accepted that specific interactions between individual lipids and proteins are crucial for function and structural integrity of membrane proteins [1,2]. Of special interest is cardiolipin (CL), the signature lipid of mitochondria. CL is essential for the function of respiratory complex III and other mitochondrial membrane proteins and also for the integrity of respiratory supercomplexes. Yet, the molecular mechanisms and underlying structure-function relationships are not understood. Disruption of CL biosynthesis leads to the Barth syndrome. This cardio-skeletal myopathy is caused by mutations in the gene of the acyl transferase tafazzin resulting ...
After obtaining a skin sample from a patient with Barth syndrome, Pu was able to make stem cells from the patients skin cells, which he then turned into heart muscle cells. What was once skin became a beating heart muscle.. Next, Pu worked with Kevin Kit Parkers bioengineering group at the Wyss Institute for Biologically Inspired Engineering to assemble the muscle cells on a tiny, flexible plastic film that instructs the cells to line up and form organized muscle fibers. When the cells are assembled into this heart-on-a-chip format, they are able to work together so their beating bends the plastic film. The amount of force the patients heart muscle generates can be measured by how much the sheet bends with each beat. Comparing a Barth syndrome patients sheet with one from a patient with a normally functioning heart further revealed that the Barth heart muscle cells were very weak compared to normal heart muscle cells. Thus, combining the stem cell and heart-on-a-chip technologies allowed ...
Barth syndrome is a rare genetic condition that mostly affects boys. Main symptoms:Heart muscle weakness (cardiomyopathy)Neutropenia (lack of white blood cells needed to fight bacterial infections).Fatigue and general muscle weakness. Growth/feeding issues Barth Syndrome UK is a well-established ...
Park, C E. and Wenner, C E., Mitochondrial lipids of eld ascites tumor cells. Abstr. (1968). Subject Strain Bibliography 1968. 223 ...
AccessGUDID - ANTI-CARDIOLIPIN IgA (04260157080133)- Anti-Cardiolipin Screen is an ELISA test system for the quantitative measurement of IgA class autoantibodies against cardiolipin in human serum or plasma. This product is intended for professional in vitro diagnostic use only.
혈액투석 환자에서 혈전형성에 의한 동정맥루 폐쇄 (vascualr access occlusion)는 가장 흔한 합병증 중의 하나로 혈관 수술 기술의 발전, 동맥경화증의 치료 및 항 혈소판 제제 사용을 포함한 약물적 치료에도 불구하고 혈액투석 환자 중 입원일수의 5-50% 정도를 차지하는 것으로 되어 있다. Anticardiolipin Antibody(ACA) 와 lupus anticoagulant(LA)는 항 인지질 항체들로 혈관 폐쇄 및 반복적인 유산의 중요한 위험인자로 알려져 있으나 혈액투석 치료를 받는 환자에서의 정확한 양성율 및 동정맥루 폐쇄와의 상관관계에 관해서는 아직 많은 이견이 있는 상태이다. 이에 저자는 이화여자대학교 부속병원에서 유지 혈액투석 중인 환자 50명, 총 68예의 동정맥루 (52 AV fistula, 16 AV grafts)를 대상으로 항 cardiolipin 항체와 루프스 항응고인자를 측정하고 병력 및 임상 기록 검토를 통해 이러한 ...
1. The [32P]phosphate incorporated into the phospholipids of isolated rat hepatic cells is present in phosphatidic acid and to a smaller extent in phosphatidylinositol. 2. The ability to synthesize nitrogen-containing phospholipids is restored by adding a liver supernatant fraction, and it is suggested that the metabolic deficiency is caused by the leakage of cytoplasmic enzymes of the synthetase system from the cells. 3. Fortified cell preparations were pulse-labelled with [32P]phosphate, [Me-14C]choline, [2-14C]ethanolamine and [U-14C]inositol and the subsequent fate of the labelled microsomal and mitochondrial phospholipids followed. 4. A fall in the specific radioactivity of microsomal phospholipids and a rise in that of mitochondrial phospholipids is interpreted as providing evidence of a transfer of labelled phospholipid molecules from the synthetic site (endoplasmic reticulum) to the mitochondrial membranes in the intact cells. 5. The formation of the phospholipids of mitochondrial ...
Quality Cardiolipin ELISA kit from ELISA kits manufacturer and elisa kits supplier: Cardiolipin ELISA kit. Our kits are FDA, CE and ISO certified.
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A Perry, Florida mom is featured in this months edition of Readers Digest for promoting awareness of Barth Syndrome. Her son suffers from the disease that attacks young children.
MILPITAS, Calif. /ePRNews/ Cardiolipin is an important phospholipid present in the inner mitochondrial membrane and constitutes about 20% of its total lipid composition. It is essential for several mitochondrial functions such as electron…
Prinn Panitchpakdi, Country Head for Thailand at CLSA, discusses the armys decision to begin paying almost $5 billion worth of rice subsidies owed to Thai farmers.
This test helps your healthcare provider diagnose clotting disorders and autoimmune diseases. It measures the concentration of antibodies related to a fat molecule in your blood.
This test helps your healthcare provider diagnose clotting disorders and autoimmune diseases. It measures the concentration of antibodies related to a fat molecule in your blood.
The first day of racing at the 2nd edition of Les Voiles de St. Barth dawned with 25 knots of tropical tradewind breeze and showers sweeping over the picturesque French island located midway down the Caribbean chain.
The McCurdy Family (clockwise from left): Steve, Will, Kate, andEliza (photo courtesy of the McCurdy family) For more on the McCurdys and the Barth Syndrome Foundation, tune in to NBCs TODAY show onMonday, February 16. From the day he was born in 1986, Will McCurdy was a bright, engaging, and alert child. Butwhen he [...]
... since β2-glycoprotein-I antibodies are a subset of anti-cardiolipin antibodies, an anti-cardiolipin assay can be performed as a ... Anti-cardiolipin IgG and/or IgM measured by standardized, non-cofactor dependent ELISA on 2 or more occasions, not less than 12 ... Anti-cardiolipin antibodies can be detected using an enzyme-linked immunosorbent assay (ELISA) immunological test, which ... Anti-ApoH and a subset of anti-cardiolipin antibodies bind to ApoH, which in turn inhibits Protein C, a glycoprotein with ...
Anti-cardiolipin. Anti-cytoplasm antibody. *Anti-neutrophil cytoplasmic *C-ANCA. *P-ANCA ...
Anti-cardiolipin. Anti-cytoplasm antibody. *Anti-neutrophil cytoplasmic *C-ANCA. *P-ANCA ...
Cardiolipins are a subclass of glycerophospholipids containing four acyl chains and three glycerol groups that are particularly ... Nowicki M, Müller F, Frentzen M (April 2005). "Cardiolipin synthase of Arabidopsis thaliana". FEBS Letters. 579 (10): 2161-5. ... Hoch FL (March 1992). "Cardiolipins and biomembrane function" (PDF). Biochimica et Biophysica Acta (BBA) - Reviews on ...
A role for cardiolipin". The Journal of Biological Chemistry. 278 (34): 31861-70. doi:10.1074/jbc.m300190200. PMID 12805375.. ...
Cardiolipin contains four fatty acids rather than two, and may help to make the inner membrane impermeable. Unlike the outer ... McMillin JB, Dowhan W (December 2002). "Cardiolipin and apoptosis". Biochimica et Biophysica Acta (BBA) - Molecular and Cell ... Additionally, the inner membrane is rich in an unusual phospholipid, cardiolipin. This phospholipid was originally discovered ...
It uses cardiolipin. The inhibitory IF1 also binds differently, in a way shared with trypanosomatida. Archaea do not generally ... Mühleip A, McComas SE, Amunts A (November 2019). "Structure of a mitochondrial ATP synthase with bound native cardiolipin". ...
... and cardiolipin is oxidized by a peroxidase function of the cardiolipin-cytochrome c complex. The hemoprotein is then detached ... Orrenius S, Zhivotovsky B (Sep 2005). "Cardiolipin oxidation sets cytochrome c free". Nature Chemical Biology. 1 (4): 188-9. ... Cytochrome c binds to cardiolipin in the inner mitochondrial membrane, thus anchoring its presence and keeping it from ... While the initial attraction between cardiolipin and cytochrome c is electrostatic due to the extreme positive charge on ...
Nowicki M (2006). Characterization of the Cardiolipin Synthase from Arabidopsis thaliana (Ph.D. Thesis thesis). RWTH-Aachen ... "Cardiolipin synthase of Arabidopsis thaliana". FEBS Letters. 579 (10): 2161-5. doi:10.1016/j.febslet.2005.03.007. PMID 15811335 ... of the inner mitochondrial membrane where it is thought to be involved in the synthesis of phosphatidylglycerol and cardiolipin ...
Other members of this family are able hydrolyze other phospholipid substrates, such as cardiolipin, or even the phosphodiester ... Nowicki M, Müller F, Frentzen M (April 2005). "Cardiolipin synthase of Arabidopsis thaliana". FEBS Letters. 579 (10): 2161-5. ... Nowicki M (2006). Characterization of the Cardiolipin Synthase from Arabidopsis thaliana (Ph.D. thesis). RWTH-Aachen University ... PLD5 PC-hydrolyzing PLD is a homologue of cardiolipin synthase, phosphatidylserine synthase, bacterial PLDs, and viral proteins ...
... and its overexpression in cultured cells resulted in increased cardiolipin synthase, activity. As cardiolipin is synthesized in ... The active t-Bid fragment is shown to localize to mitochondria through a positive interaction with cardiolipin. This activated ... M. Nowicki (2006). "Characterization of the Cardiolipin Synthase from Arabidopsis thaliana". Ph.D. Thesis, RWTH-Aachen ... Recent findings suggest that PLSCR3 is involved in regulation of biosynthesis of cardiolipin in mitochondria, ...
A membrane lipid cardiolipin is exclusively found in the inner mitochondrial membrane and bacterial cell membranes.[58] ... that cardiolipin is found only in the inner mitochondrial membrane and bacterial cell membranes; and that some mitochondria and ... "Cardiolipin membrane domains in prokaryotes and eukaryotes". Biochimica et Biophysica Acta (BBA) - Biomembranes. 1788 (10): ...
A membrane lipid cardiolipin is exclusively found in the inner mitochondrial membrane and bacterial cell membranes.[58] ... that cardiolipin is found only in the inner mitochondrial membrane and bacterial cell membranes; and that some mitochondria and ... "Cardiolipin membrane domains in prokaryotes and eukaryotes". Biochim Biophys Acta. 1788 (10): 2084-2091. doi:10.1016/j.bbamem. ...
Ye C, Shen Z, Greenberg ML (April 2016). "Cardiolipin remodeling: a regulatory hub for modulating cardiolipin metabolism and ... Barth syndrome patients exhibit defects in cardiolipin metabolism, including aberrant cardiolipin fatty acyl composition, ... After its synthesis, cardiolipin cannot exert its proper functions until it is actively remodeled. Tafazzin, an acyl-specific ... Cardiolipin is a complex glycerophospholipid which contains 4 acyl groups linked to three glycerol moietie localized in the ...
Mileykovskaya, E; Dowhan, W (2009). "Cardiolipin Membrane Domains in Prokaryotes and Eukaryotes". Biochimica et Biophysica Acta ...
One of its functions is to bind cardiolipin. When bound, the structure of cardiolipin and β2-GP1 both undergo large changes in ... Borchman D, Harris EN, Pierangeli SS, Lamba OP (1995). "Interactions and molecular structure of cardiolipin and beta 2- ... systemic lupus erythematosus (SLE)). Positivity for anti-cardiolipin antibodies in diagnostic tests for autoimmune ... beta 2-glycoprotein I identifies a cluster of lysine residues that are critical for phospholipid binding and anti-cardiolipin ...
Identify via agglutination with cardiolipin sensitive chicken erythrocytes. RCN infected Strain 143 human osteosarcoma cells ...
In addition it contains cardiolipin and phosphatidylcholine as the major phospholipids, while sterols are absent. Cardiolipin ...
Alpha helices 2 and 3 bind to cardiolipin. The UQCC3 gene encodes a protein that functions in complex III assembly, downstream ... Desmurs M, Foti M, Raemy E, Vaz FM, Martinou JC, Bairoch A, Lane L (April 2015). "C11orf83, a mitochondrial cardiolipin-binding ... Depletion of this protein alters cardiolipin composition, causing cellular and mitochondrial defects. The UQCC3 gene is located ... Depleted levels of the UQCC3 protein cause impaired respiration and subtle yet significant alterations in cardiolipin ...
Yadav, Pradeep Kumar; Rajasekharan, Ram (2017). "Cardiolipin deficiency causes triacylglycerol accumulation in Saccharomyces ...
Cardiolipin is intimately connected with the electron transport chain proteins and the membrane structure of the mitochondria ... Kulik found that all the BTHS individuals that he tested had abnormalities in their cardiolipin molecules, a lipid found inside ... Claypool SM, Boontheung P, McCaffery JM, Loo JA, Koehler CM (December 2008). "The cardiolipin transacylase, tafazzin, ... December 2000). "Defective remodeling of cardiolipin and phosphatidylglycerol in Barth syndrome". Biochemical and Biophysical ...
Phosphatidylcholine makes up about 26.5%, cardiolipin 25.4%, and phosphatidylinositol 4.5%. In S. cerevisiae mitochondria, ... cardiolipin 16.1%, phosphatidylserine 3.8%, and phosphatidic acid 1.5%. In the inner mitochondrial membrane, the protein-to- ...
Although MLCL AT-1 and MTP are different proteins, in 2012 the same lab discovered that MTP did indeed have cardiolipin ... This suggests a possible link between mitochondrial membrane cardiolipin content and beta oxidation. Disorders associated MTP ... "Human Trifunctional Protein Alpha Links Cardiolipin Remodeling to Beta-Oxidation". PLOS ONE. 7 (11): e48628. Bibcode:2012PLoSO ... linking MTP to the remodeling of cardiolipin from monolysocardiolipin. ...
... into cardiolipin. In 1990, biologists Michael Schlame and Bernd Rustow observed the deacylation of cardiolipin into MLCL, which ... producing cardiolipin. This lipid remodeling is separate from the cardiolipin synthesis pathway, and is essential to maintain ... Since cardiolipin content is significantly diminished in the inner mitochondrial membrane during apoptosis, the increase of ... Taylor WA, Mejia EM, Mitchell RW, Choy PC, Sparagna GC, Hatch GM (2012). "Human trifunctional protein alpha links cardiolipin ...
... and in particular requires the presence of cardiolipin, a unique mitochondrial lipid. In yeast mitochondria lacking cardiolipin ... Zhang, M. (2005). "Cardiolipin Is Essential for Organization of Complexes III and IV into a Supercomplex in Intact Yeast ... 2009), cardiolipin stabilizes the supercomplex formation by neutralizing the charges of lysine residues in the interaction ... CARDIOLIPIN IS REQUIRED FOR SUPERCOMPLEX FORMATION IN THE INNER MITOCHONDRIAL MEMBRANE". Journal of Biological Chemistry. 277 ( ...
This is a family of proteins that acts as a mitochondrial phosphatase in cardiolipin biosynthesis. Cardiolipin is a unique ... "A mitochondrial phosphatase required for cardiolipin biosynthesis: the PGP phosphatase Gep4". The EMBO Journal. 29 (12): 1976- ...
Nowicki M, Müller F, Frentzen M (April 2005). "Cardiolipin synthase of Arabidopsis thaliana". 》FEBS Letters》 579 (10): 2161-5. ... "Cardiolipins and biomembrane function" (PDF). 》Biochimica et Biophysica Acta》 1113 (1): 71-133. doi:10.1016/0304-4157(92)90035 ...
The envelope contains a variety of lipids including cardiolipins, phosphatidylglycerols, phosphatidylglycerophosphate methyl ...
Cardiolipin antibody tests help diagnose the cause of blood clots, recurrent miscarriages or antiphospholipid syndrome (APS). ... Cardiolipin (anticardiolipin) antibodies are autoantibodies that can affect blood clotting. ... Cardiolipin antibodies are autoantibodies produced by the immune system that mistakenly target the bodys own cardiolipins, ... Cardiolipin antibodies target cardiolipins and are associated with an increased risk of developing recurrent inappropriate ...
... has displayed the importance of cardiolipin in mitochondrial functionality. Cardiolipin effects mitochondrial bioenergetics by ... Changes in cardiolipin concentrations and alterations to its acyl groups have led to a variety of pathological conditions such ... Specifically, interaction of cardiolipin with cytochrome oxidase and adenine nucleotide translocase is required in order for ... a disease associated with abnormalities in the fatty acid composition of cardiolipin causing cardiomyopathy and neutropenia, ...
Cardiolipin from a cow heart is used as an antigen in the Wassermann test for syphilis. Anti-cardiolipin antibodies can also be ... 2009;23(4):210-2. Cardiolipin at the US National Library of Medicine Medical Subject Headings (MeSH) Cardiolipin ( ... Phospholipase D (PLD) in the mitochondrion hydrolyses cardiolipin to phosphatidic acid. Because of cardiolipins unique ... The enzyme can operate in reverse under some physiological conditions to remove cardiolipin. Catabolism of cardiolipin may ...
cardiolipin synonyms, cardiolipin pronunciation, cardiolipin translation, English dictionary definition of cardiolipin. n. A ... cardiolipin. Also found in: Medical, Encyclopedia, Wikipedia.. Related to cardiolipin: Lupus anticoagulant ... NeoPharm uses a proprietary synthetic form of cardiolipin. In its natural form, cardiolipin occurs as a negatively charged ... Cardiolipin - definition of cardiolipin by The Free Dictionary https://www.thefreedictionary.com/cardiolipin ...
Cardiolipin_synth_ClsA (MF_00190). Accession MF_00190 Integration. Cardiolipin synthase A (IPR030840) Member database. HAMAP. ...
Anti-cardiolipin antibodies (ACA) are antibodies often directed against cardiolipin and found in several diseases, including ... Anti-cardiolipin antibodies can be classified in two ways: As IgM, IgG or IgA As β2-glycoprotein dependent or independent In ... In SLE, anti-DNA antibodies and anti-cardiolipin antibodies may be present individually or together; the two types of ... Only a subset of autoimmune anti-cardiolipin antibodies bind Apo-H, these anti-apolipoprotein antibodies are associated with ...
... ,ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative ... Anti-Cardiolipin Antibodies, IgG & IgM. 2. Anti-Cardiolipin Antibody, IgG. 3. Adenovirus Antibody, IgG. 4. Adenovirus Antibody ...
ACA - aCL Antibody - aCL IgA - aCL IgM - Anticardiolipin Antibodies - Cardiolipin - Anti-Cardiolipin Antibody ... For health professionals : Refer a Patient : Laboratory Services : Test Table : Cardiolipin Antibodies, IgG, IgM ... Anti-cardiolipin antibodies have been strongly associated with venous and arterial thrombosis. In patients with SLE, a ... Presence of antiphospholipid antibody syndrome should be considered if cardiolipin IgG and/or IgM antibody is present at ...
Members of the mitochondrial carrier family interact with cardiolipin (CL) as evident from a variety of functional and ... Cardiolipin and mitochondrial carriers Biochim Biophys Acta. 2009 Oct;1788(10):2048-58. doi: 10.1016/j.bbamem.2009.06.007. Epub ... Members of the mitochondrial carrier family interact with cardiolipin (CL) as evident from a variety of functional and ...
C. Ye, Z. Shen, and M. L. Greenberg, "Cardiolipin remodeling: a regulatory hub for modulating cardiolipin metabolism and ... The Role of Cardiolipin in Cardiovascular Health. Zheni Shen,1 Cunqi Ye,1,2 Keanna McCain,1 and Miriam L. Greenberg1 ... A. S. Joshi, J. Zhou, V. M. Gohil, S. Chen, and M. L. Greenberg, "Cellular functions of cardiolipin in yeast," Biochimica et ... Figure 1: Cardiolipin synthesis and remodeling pathway in humans and yeast. Phosphatidic acid (PA) is converted to CDP- ...
The ImmunoWELL Cardiolipin Antibody (IgM) Test is an enzyme immunoassay (EIA) for the quantitative detection of antibodies to ... cardiolipin antigen in serum and is used as an aid for assessing the risk of thrombosis in individuals with systemic lupus ... ImmunoWELL Cardiolipin Antibody (IgM) Test. Features. *ImmunoWELL products use the industry standard microtiter plate format. * ... ImmunoWELL Cardiolipin Antibody (IgG) Test. 2. ImmunoWELL TPO IgG Quantitative Test. 3. ImmunoWELL Thyroglobulin IgG ...
Cardiolipins have multiple vital functions within biological cell membranes, most notably in the energy metabolism associated ... Considering their essential role, peroxidation of cardiolipins may plaus... ... How cardiolipin peroxidation alters the properties of the inner mitochondrial membrane?. *Vähäheikkilä M ... Cardiolipins have multiple vital functions within biological cell membranes, most notably in the energy metabolism associated ...
Cardiolipin (CL) is a structurally unique dimeric phospholipid localized in the inner mitochondrial membrane where it is ... Role of cardiolipin alterations in mitochondrial dysfunction and disease Am J Physiol Cell Physiol. 2007 Jan;292(1):C33-44. doi ... Cardiolipin (CL) is a structurally unique dimeric phospholipid localized in the inner mitochondrial membrane where it is ...
Cardiolipins Are Biomarkers of Mitochondria-Rich Thyroid Oncocytic Tumors. Jialing Zhang, Wendong Yu, Seung Woo Ryu, John Lin, ... Cardiolipins Are Biomarkers of Mitochondria-Rich Thyroid Oncocytic Tumors. Jialing Zhang, Wendong Yu, Seung Woo Ryu, John Lin, ... Cardiolipins Are Biomarkers of Mitochondria-Rich Thyroid Oncocytic Tumors. Jialing Zhang, Wendong Yu, Seung Woo Ryu, John Lin, ... Cardiolipin fingerprinting of leukocytes by MALDI-TOF/MS as a screening tool for Barth syndrome. J Lipid Res 2015;56:1787-94. ...
Cardiolipin antibodies are associated with the lupus inhibitor, beta-2-glycoprotein1 antibodies, and with false positive ... Moderate to high levels of IgG cardiolipin antibody are seen in the Phospholipid antibody syndrome, but the antibody may also ...
Instead, cardiolipin mediates ABCA1-dependent susceptibility to podocyte injury, as inhibition of cardiolipin peroxidation with ... We demonstrated that this pathway is relevant to DKD and that ABCA1 inducers or inhibitors of cardiolipin peroxidation may each ... Collectively, we describe a pathway definitively linking ABCA1 deficiency to cardiolipin-driven mitochondrial dysfunction. ... ATP-binding cassette A1 deficiency causes cardiolipin-driven mitochondrial dysfunction in podocytes. ...
... a cardiolipin, and at least one antioxidant, wherein the composition is useful for improving, maintaining, or restoring a ... wherein the cardiolipin is one of tetraoleoyl-cardiolipin, tetrapalmitoleoyl-cardiolipin, or tetramyristoyl-cardiolipin and is ... the cardiolipin is, for example, one of tetraoleoyl-cardiolipin, tetrapalmitoleoyl-cardiolipin, or tetramyristoyl-cardiolipin; ... Cardiolipin. Cardiolipin is embedded in the liposome of the present invention for delivery to mitochondria. Cardiolipin is ...
... cardiolipin (10). In light of these observations, we assayed 2F5 and 4E10 mAbs, along with other mAbs to HIV-1, for cardiolipin ... Unlike antibodies to cardiolipin found in SLE, "infectious" antibodies to cardiolipin, such as those found in HIV-1 infection, ... Cardiolipin HEp-2 cell reactivity Ro (SSA) dsDNA Centromere B Histones Membrane-proximal external region (2F5) 47 +Cytoplasmic ... expressing the 4E10 epitope inhibit binding of 4E10 mAb to cardiolipin. The IC50 of inhibition of 4E10 binding to cardiolipin ...
The antibodies attack cardiolipins by mistake. Cardiolipin is a phospholipid, or a kind of fat in the blood that is important ... Cardiolipin Antibody. Does this test have other names?. Cardiolipin antibodies (IgG, IgM, IgA), anticardiolipin, aCL antibody ... When high levels of cardiolipin antibodies are found in people with these or other issues, it is known as cardiolipin antibody ... The results of a cardiolipin antibody test are easy to understand. If youre negative for cardiolipin antibodies, that is ...
Cardiolipin antibodies (IgG, IgM, IgA), anticardiolipin What is this test?. A cardiolipin antibodies test looks for a certain ... When high levels of cardiolipins are found in people with these or other issues, it is known as cardiolipin antibody syndrome. ... The results of a cardiolipin antibodies test are easy to understand. If youre negative for cardiolipin antibodies, that is ... They are antibodies that form in response to cardiolipins. Cardiolipin is a phospholipid, or a kind of fat in the blood. The ...
The most commonly used phospholipid substrate is cardiolipin. The term phospholipid antibody is actually a misnomer. The ... These autoantibodies are called phospholipid or cardiolipin antibodies when they are detected by immunoassays that employ ... and most immunoassays for phospholipid antibodies employ a composite substrate consisting of cardiolipin plus beta-2 GP1. Beta- ... An IgA phospholipid (cardiolipin) antibody result above 15 APL with negative IgG and IgM phospholipids (cardiolipin) antibody ...
Binding of Cardiolipin to c-Rings.. Cardiolipin, in strong preference to phosphatidyl lipids, became bound to native c8-rings ... Role of Cardiolipin Molecules in ATP Synthase.. The sites where cardiolipins are bound permanently to complexes III and IV and ... 1). Cardiolipin remained longer in the inner annulus than phosphatidyl lipids. Once a cardiolipin entered the lipid annulus in ... Each gray line represents a cardiolipin, and colored bars along the line indicate when a cardiolipin phosphate bound to a c- ...
... a disorder of cardiolipin metabolism) induces mitochondrial dysfunction and cardiomyopathy, and since decreases in cardiolipin ... Shotgun lipidomics identifies cardiolipin depletion in diabetic myocardium linking altered substrate utilization with ... cardiolipin, in diabetic murine myocardium (from 7.2 +/- 0.3 nmol/mg of protein in control hearts to 3.1 +/- 0.1 nmol/mg of ... cardiolipin depletion, mitochondrial dysfunction, and resultant hemodynamic compromise. ...
Catalyzes the synthesis of cardiolipin (CL) (diphosphatidylglycerol) by specifically transferring a phosphatidyl group from CDP ... cardiolipin biosynthetic process Source: WormBaseInferred from mutant phenotypei*. "Deficiency of cardiolipin synthase causes ... Probable cardiolipin synthase (CMP-forming)Add BLAST. 246. Proteomic databases. PaxDb, a database of protein abundance averages ... Probable cardiolipin synthase (CMP-forming) (EC:2.7.8.41*Search proteins in UniProtKB for this EC number. ...
Cardiolipin Background[edit]. Cardiolipin formation is key to the enzyme functionality of the mitochondria thereby with it ... Secondly, Cld1 can add water to phospholipids as well as cardiolipin. Formation Cardiolipin and Functionality[edit]. This ... Cardiolipin contains two phosphatidyl groups connected by a glycerol. For this reason, cardiolipin is a lipid dimer that has ... Cardiolipin phospholipases The removal of a single acyl chain leads to monolysocardiolipin, which initiates cardiolipin ...
Cardiolipin is an inner mitochondrial membrane phospholipid. We found that rotenone, staurosporine, 6-hydroxydopamine and other ... pro-mitophagy stimuli caused externalization of cardiolipin to the mitochondrial surface in primary cortical neurons and SH ... RNAi knockdown of cardiolipin synthase or of phospholipid scramblase-3, which transports cardiolipin to the outer mitochondrial ... Cardiolipin externalization to the outer mitochondrial membrane acts as an elimination signal for mitophagy in neuronal cells. ...
Similar to OPA1, Nm23-H4 is further known to strongly bind in vitro to anionic phospholipids, mainly cardiolipin, and in vivo ... We found that wild type, but not the mutant enzyme, selectively increased the content of cardiolipin in the outer mitochondrial ... Dual function of mitochondrial Nm23-H4 protein in phosphotransfer and intermembrane lipid transfer: a cardiolipin-dependent ... Nm23-H4 or a membrane binding-deficient mutant at a site predicted based on molecular modeling to be crucial for cardiolipin ...
Anti-Cardiolipin Antikörper (IgG in GPL-U/ml und IgM in MPL-U/ml) ... PBL Fluoroenzyme-Immunoassay FOR ANTI Cardiolipin - ANTIBODIES IgM Phadia GmbH 250-5530-021. ... PBL Fluoroenzyme-Immunoassay FOR ANTI Cardiolipin - ANTIBODIES IgG Phadia GmbH 250-5529-021 ...
Catalyzes the phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) ( ... Cardiolipin synthase BAdd BLAST. 413. Proteomic databases. PaxDb, a database of protein abundance averages across all three ... Cardiolipin synthase subfamily. ClsB sub-subfamily.UniRule annotation. Manual assertion according to rulesi ... 2 1,2-diacyl-sn-glycero-3-phospho-(1-sn-glycerol) = a cardiolipin + glycerol.UniRule annotation. Manual assertion according to ...
To determine whether cardiolipin is essential for yeast growth, we generated a cardiolipin synthase null mutant by disrupting ... Biochemical analysis of the mutant indicated that it had no cardiolipin synthase activity and no cardiolipin in its membranes. ... Cardiolipin is not essential for the growth of Saccharomyces cerevisiae on fermentable or non-fermentable carbon sources.. ... Cardiolipin is a unique dimeric phospholipid, which is present throughout the eukaryotic kingdom and is specifically localized ...
  • Cardiolipin is found predominantly in the membranes of mitochondria and bacteria. (sigmaaldrich.com)
  • Cardiolipin effects mitochondrial bioenergetics by providing essential structural and functional support to proteins localized within the mitochondria. (sigmaaldrich.com)
  • Cardiolipin bicyclic structure Structure of NAO NAO & CL arranged in a highly ordered way In eukaryotes such as yeasts, plants and animals, the synthesis processes are believed to happen in mitochondria. (wikipedia.org)
  • Finally, a molecule of CDP-DAG is bound to PG to form one molecule of cardiolipin, catalyzed by the mitochondria-localized enzyme cardiolipin synthase (CLS). (wikipedia.org)
  • Mitochondrial mass determination: The preferential binding of NAO to the cardiolipin moiety in mitochondria was exploited to estimate mitochondrial mass (23,25). (thefreedictionary.com)
  • Cardiolipin (CL), the signature phospholipid of mitochondrial membranes, is crucial for both mitochondrial function and cellular processes outside of the mitochondria. (hindawi.com)
  • The inner mitochondrial membrane of eukaryotic cells has a complex structure and molecular composition, recognized by the presence of cardiolipins (CL), a unique class of anionic glycerophospholipid located predominantly (if not exclusively) in mitochondria. (aacrjournals.org)
  • The anionic lipid cardiolipin, a component of bacterial membranes and the inner membranes of mitochondria where the machine is found, is an essential component of the enzyme. (pnas.org)
  • Cardiolipin is associated uniquely with energy-transducing membranes in mitochondria and eubacteria. (pnas.org)
  • Cardiolipin consists of two 3-phosphatidyl groups linked by a glycerol bridge, and in bovine mitochondria, the four acyl chains have 18 carbon atoms with one or two unsaturated linkages ( 4 ). (pnas.org)
  • Cardiolipin is known as the signature phospholipid of mitochondria. (wikibooks.org)
  • Cardiolipin formation is key to the enzyme functionality of the mitochondria thereby with it malfunctioning mitochondria related diseases can occur. (wikibooks.org)
  • There, there is no surprise that most of the cardiolipin in a cell is closely tied to the inner membrane of mitochondria. (wikibooks.org)
  • Furthermore, the cardiolipin synthesis actually takes place in the inner membrane of mitochondria. (wikibooks.org)
  • Because of this close association between cardiolipin and mitochondria, it is hypothesized that cardiolipin plays a huge role in ATP production through oxidative phosphorylation. (wikibooks.org)
  • RNAi knockdown of cardiolipin synthase or of phospholipid scramblase-3, which transports cardiolipin to the outer mitochondrial membrane, decreased the delivery of mitochondria to autophagosomes. (cdc.gov)
  • Furthermore, we found that the autophagy protein microtubule-associated-protein-1 light chain 3 (LC3), which mediates both autophagosome formation and cargo recognition, contains cardiolipin-binding sites important for the engulfment of mitochondria by the autophagic system. (cdc.gov)
  • These data indicate that redistribution of cardiolipin serves as an 'eat-me' signal for the elimination of damaged mitochondria from neuronal cells. (cdc.gov)
  • Cardiolipin is required for the integrity of several protein complexes in the IM, including the TIM23 translocase, a dynamic complex which mediates protein import into the mitochondria through interactions with the import motor presequence translocase-associated motor (PAM). (rupress.org)
  • In ups1Δ mitochondria, cardiolipin levels were decreased, and the TIM23 translocase showed altered conformation and decreased association with PAM, leading to defects in mitochondrial protein import. (rupress.org)
  • Strikingly, loss of Ups2p restored normal cardiolipin levels and rescued TIM23 defects in ups1Δ mitochondria. (rupress.org)
  • Damaged and dysfunctional mitochondria generate increased levels of reactive oxygen species and we hypothesized that these cause the oxidation of the mitochondrial membrane poly-unsaturated lipid, cardiolipin (CL), which acts as an indicator of mitochondrial health and as an initiating signal to the mitophagic machinery. (gla.ac.uk)
  • One of the first signals of the apoptotic pathway within mitochondria is oxidized cardiolipin [5, 13, 20, 21]. (thefreelibrary.com)
  • Thus, here we tested the hypothesis of retinal cardiolipin metabolism modification in mitochondria responsible for these respiratory chain alterations. (arvojournals.org)
  • Remodeling of the acyl chains of cardiolipin (CL) is responsible for final molecular composition of mature CL after de novo CL synthesis in mitochondria. (nii.ac.jp)
  • We highlight the role of cardiolipin for the respiratory chain, metabolite carriers, and mitochondrial metabolism and describe links to apoptosis and mitochondria specific autophagy (mitophagy) with possible implications in cardiac disease. (mpg.de)
  • Cardiolipins, and other related phospholipids , are lipid molecules that play an important role in the blood clotting process. (labtestsonline.org)
  • Cardiolipin (IUPAC name 1,3-bis(sn-3'-phosphatidyl)-sn-glycerol) is an important component of the inner mitochondrial membrane, where it constitutes about 20% of the total lipid composition. (wikipedia.org)
  • Cardiolipin (CL) is a kind of diphosphatidylglycerol lipid. (wikipedia.org)
  • In its natural form, cardiolipin occurs as a negatively charged lipid found in cardiac tissue. (thefreedictionary.com)
  • Cardiolipin (CL) is the signature lipid of mitochondrial membranes. (hindawi.com)
  • Considering their essential role, peroxidation of cardiolipins may plausibly have significant effects, as peroxidation is known to alter the functionality of lipid molecules. (mendeley.com)
  • The anionic lipid cardiolipin is an essential component of active ATP synthases. (pnas.org)
  • Since Barth's syndrome (a disorder of cardiolipin metabolism) induces mitochondrial dysfunction and cardiomyopathy, and since decreases in cardiolipin content precipitate mitochondrial dysfunction, these results provide a unifying hypothesis linking altered substrate utilization and metabolic flux in diabetic myocardium with altered lipid metabolism, cardiolipin depletion, mitochondrial dysfunction, and resultant hemodynamic compromise. (nih.gov)
  • For this reason, cardiolipin is a lipid dimer that has four acyl chains instead of two as seen in other phospholipids. (wikibooks.org)
  • Dual function of mitochondrial Nm23-H4 protein in phosphotransfer and intermembrane lipid transfer: a cardiolipin-dependent switch. (cdc.gov)
  • Mitochondrial lipid distribution was analyzed by liquid chromatography-mass spectrometry using HeLa cells expressing either wild-type Nm23-H4 or a membrane binding-deficient mutant at a site predicted based on molecular modeling to be crucial for cardiolipin binding and transfer mechanism. (cdc.gov)
  • Based on these data and molecular modeling, we propose that Nm23-H4 acts as a lipid-dependent mitochondrial switch with dual function in phosphotransfer serving local GTP supply and cardiolipin transfer for apoptotic signaling and putative other functions. (cdc.gov)
  • Lower FASN activity in PINK1 mutants decreases palmitate levels and increases the levels of cardiolipin (CL), a mitochondrial inner membrane-specific lipid. (rupress.org)
  • Not only oxidation of cardiolipin affects the affinity of cytochrome C for lipid bilayers. (sigmaaldrich.com)
  • These observations suggest that doxorubicin has a sex-specific impact on the heart phospholipidome especially on cardiolipin, an essential mitochondrial lipid. (biomedcentral.com)
  • Phosphatidic acid (PA) and Cardiolipin (CAR) in the phospholipids fraction were not identified in the [sup.1]H-NMR spectrum, as head groups of these two phospholipids were not very different from the glycerol moiety. (thefreedictionary.com)
  • Several researchers have studied mitochondrial membrane lipids from many organisms, observing mainly phospholipids ( cardiolipin , phosphatidilethanolamine, phosphatidilserine) and cholesterol. (thefreedictionary.com)
  • These autoantibodies are called phospholipid or cardiolipin antibodies when they are detected by immunoassays that employ anionic phospholipids as substrates. (mayomedicallaboratories.com)
  • Unlike other phospholipids that are also synthesized in a special compartment of the cell, cardiolipin remains closely associated with the mitochondrial membranes and does not get distributed out to the other endomembrane system like other phospholipids do. (wikibooks.org)
  • Similar to OPA1, Nm23-H4 is further known to strongly bind in vitro to anionic phospholipids, mainly cardiolipin, and in vivo to the inner mitochondrial membrane. (cdc.gov)
  • We found that wild type, but not the mutant enzyme, selectively increased the content of cardiolipin in the outer mitochondrial membrane, but the distribution of other more abundant phospholipids (e.g. phosphatidylcholine) remained unchanged. (cdc.gov)
  • Indeed, cardiolipins are specific phospholipids of the inner mitochondrial membrane which have a key role in the stabilization of respiratory chain supercomplexes and in particular mitochondrial complex IV. (arvojournals.org)
  • rotation measurements and chromatography of the intact phospholipids and deacylated products indicated that beef-heart cardiolipin has a diphosphatidyl glycerol structure. (uu.nl)
  • Anti-cardiolipin (aCL) autoimmune antibodies are a subset of antibodies which react with negatively charged phospholipids. (genbio.com)
  • The molecule's positive charge allows for binding to phospholipids and cardiolipin, both of which can be found as epitopes on the surfaces of pathogens, and its 2nd and 3rd helices are principle players in lysing foreign or infected cells. (wikipedia.org)
  • Cardiolipin antibodies are autoantibodies produced by the immune system that mistakenly target the body's own cardiolipins, substances found in the outermost layer of cells (cell membranes) and platelets. (labtestsonline.org)
  • Cardiolipin antibodies are the most common antiphospholipid antibody , a group of autoantibodies associated with excessive clotting and autoimmune diseases , such as lupus . (labtestsonline.org)
  • Here we demonstrate that the two most broadly reactive HIV-1 envelope gp41 human mAbs, 2F5 and 4E10, are polyspecific autoantibodies reactive with the phospholipid cardiolipin. (sciencemag.org)
  • Long hydrophobic CDR3 regions are generally typical of poly-reactive autoantibodies ( 9 ), and HIV-1-infected patient B lymphocytes are driven to make polyclonal antibodies with reactivity to the endogenous phospholipid, cardiolipin ( 10 ). (sciencemag.org)
  • Anti- Cardiolipin autoantibodies (ACA) are frequently found in patients with SLE. (rapidtest.com)
  • The ZEUS ELISA Cardiolipin IgA Test System is designed for the semi-quantitative measurement of circulating IgA autoantibodies to cardiolipin. (zeusscientific.com)
  • An enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of circulating IgG, IgA and IgM autoantibodies to cardiolipin. (vwr.com)
  • Anti-Cardiolipin Screen is an ELISA test system for the quantitative measurement of IgA class autoantibodies against cardiolipin in human serum or plasma. (nih.gov)
  • Cardiolipins have multiple vital functions within biological cell membranes, most notably in the energy metabolism associated with the inner mitochondrial membrane. (mendeley.com)
  • In computer simulations of hydrated membranes containing trimethylated or unmethylated bovine c 8 -rings and bacterial c 10 - or c 11 -rings, the head-groups of cardiolipin molecules became associated selectively with these modified and unmodified lysine residues and with adjacent polar amino acids and with a second conserved lysine on the opposite side of the membrane, whereas phosphatidyl lipids were attracted little to these sites. (pnas.org)
  • In addition to this, since cardiolipin is only found in membranes that has an electrochemical gradient, it therefore also supports that cardiolipin has something to do with ATP production in the powerhouse of the cell. (wikibooks.org)
  • Cardiolipin is a unique dimeric phospholipid, which is present throughout the eukaryotic kingdom and is specifically localized in mitochondrial membranes. (nih.gov)
  • Biochemical analysis of the mutant indicated that it had no cardiolipin synthase activity and no cardiolipin in its membranes. (nih.gov)
  • Cardiolipin (CL) is the signature phospholipid of mitochondrial membranes, where it is synthesized locally and plays a critical role in mitochondrial bioenergetic functions. (wayne.edu)
  • The encoded enzyme catalyzes the synthesis of cardiolipin, a phospholipid component of mitochondrial membranes that is critical for mitochondrial function. (nih.gov)
  • Mitochondrial membranes are unique in the cell as they contain the phospholipid cardiolipin. (mpg.de)
  • However, apoptosis can also be initiated by GNLY, due to the presence of cardiolipin in mitochodrial membranes which allows GNLY to create pores in the membrane and causing a release of molecules like cytochrome c, which also leads to apoptosis. (wikipedia.org)
  • In mammalian cells, but also in plant cells, cardiolipin (CL) is found almost exclusively in the inner mitochondrial membrane, where it is essential for the optimal function of numerous enzymes that are involved in mitochondrial energy metabolism. (wikipedia.org)
  • How cardiolipin peroxidation alters the properties of the inner mitochondrial membrane? (mendeley.com)
  • We used atomistic molecular dynamics simulations to study how peroxidation of cardiolipin affects the properties of the inner mitochondrial membrane. (mendeley.com)
  • To this end, we explored what happens when varying fractions of fatty acid chains of cardiolipin are replaced by its four different oxidized products in systems modeling the inner mitochondrial membrane. (mendeley.com)
  • Cardiolipin (CL) is a structurally unique dimeric phospholipid localized in the inner mitochondrial membrane where it is required for optimal mitochondrial function. (nih.gov)
  • Cardiolipin is an inner mitochondrial membrane phospholipid. (cdc.gov)
  • Cardiolipin, a unique phospholipid is present and synthesised in the inner mitochondrial membrane. (sigmaaldrich.com)
  • Cardiolipin is very much essential for the functional activity of several inner mitochondrial membrane proteins, like respiratory chain complexes. (sigmaaldrich.com)
  • Cardiolipin (CL) is a unique dimeric phospholipid that exists almost exclusively in the inner mitochondrial membrane (IMM) in eukaryotic cells. (pubfacts.com)
  • Biochemical abnormalities include decreased levels of the mitochondrial phospholipid cardiolipin, increased levels of monolysocardiolipin, and a lower degree of unsaturation of the (monolyso)cardiolipin acyl chains. (ru.nl)
  • DI-fusion Holdings: Saturation of acyl chains converts cardiolipin from. (ac.be)
  • Therefore, disorientation and disturbance in the metabolism of cardiolipin can cause pathological issues. (wikibooks.org)
  • The following will discuss newly found molecules involved in cardiolipin metabolism as well as how human diseases are affected by the changes in cardiolipin metabolism. (wikibooks.org)
  • We do know that the Barth syndrome is associated with the first inborn error of cardiolipin metabolism, since its cardiolipin is alternated in biosynthesis. (wikibooks.org)
  • In this study, we report that two homologous intermembrane space proteins, Ups1p and Ups2p, control cardiolipin metabolism and affect the assembly state of TIM23 and its association with PAM in an opposing manner. (rupress.org)
  • Our findings provide a novel molecular mechanism for the regulation of cardiolipin metabolism. (rupress.org)
  • This seminar will highlight studies of a specific mitochondrial membrane component, cardiolipin, which may serve as a molecular bridge connecting altered metabolism and resistance to cell death. (calvin.edu)
  • Retinal cardiolipin metabolism is altered in a mouse model of Dominant Optic Atrophy. (arvojournals.org)
  • Thus, our results showed that Opa1 haploinsufficiency impacts cardiolipin metabolism in the retina. (arvojournals.org)
  • Growing evidence links phospholipid alterations especially cardiolipins (CLs) to defects in mitochondrial function and energy metabolism in heart failure. (biomedcentral.com)
  • Presence of antiphospholipid antibody syndrome should be considered if cardiolipin IgG and/or IgM antibody is present at moderate or above level on two or more occasions, at least 12 weeks apart. (legacyhealth.org)
  • Moderate to high levels of IgG cardiolipin antibody are seen in the Phospholipid antibody syndrome, but the antibody may also be detected in SLE, other autoimmune disorders, some infections (eg, Syphilis) and with some drugs. (edu.au)
  • When high levels of cardiolipin antibodies are found in people with these or other issues, it is known as cardiolipin antibody syndrome. (ahealthyme.com)
  • If you're positive, you might have cardiolipin antibody syndrome. (ahealthyme.com)
  • This is to give accurate results for lupus or cardiolipin antibody syndrome. (ahealthyme.com)
  • Cardiolipin antibodies are useful in identifying patients with an increased risk of thrombosis, recurrent spontaneous abortions and phospholipid antibody syndrome. (lalpathlabs.com)
  • In prokaryotes such as bacteria, diphosphatidylglycerol synthase catalyses a transfer of the phosphatidyl moiety of one phosphatidylglycerol to the free 3'-hydroxyl group of another, with the elimination of one molecule of glycerol, via the action of an enzyme related to phospholipase D. The enzyme can operate in reverse under some physiological conditions to remove cardiolipin. (wikipedia.org)
  • These tests, also referred to as phospholipid antibody tests, use various techniques, such as flocculation tests or enzyme-linked immunosorbent assays (ELISA), in order to detect antibodies that target reagin, which is a complex of cardiolipin , lecithin, and cholesterol. (thefreedictionary.com)
  • The ImmunoWELL Cardiolipin Antibody (IgM) Test is an enzyme immunoassay (EIA) for the quantitative detection of antibodies to cardiolipin antigen in serum and is used as an aid for assessing the risk of thrombosis in individuals with systemic lupus erythematosus (SLE) and lupus-like disorders. (bio-medicine.org)
  • However, the residence times of cardiolipin molecules with the ring were brief and sufficient for the rotor to turn only a fraction of a degree in the active enzyme. (pnas.org)
  • These highly specific but brief interactions with the rotating c-ring are consistent with functional roles for cardiolipin in stabilizing and lubricating the rotor, and, by interacting with the enzyme at the inlet and exit of the transmembrane proton channel, in participation in proton translocation through the membrane domain of the enzyme. (pnas.org)
  • The enzyme phosphatidylglycerolphosphate synthase, which catalyses the committed step of the cardiolipin pathway, remained unaffected in the null mutant. (nih.gov)
  • The Diagnostic Automation Cardiolipin IgA Enzyme-linked Immunosorbent Assay (ELISA), is intended for the detection and semiquantitative determination of IgA antibodies to Cardiolipin in human sera or plasma. (rapidtest.com)
  • LS-F41209 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Porcine Anti-Cardiolipin antibody (IgM). (lsbio.com)
  • The anionic cardiolipin tightly associates with the NarGHI complex and is the most effective phospholipid to restore functionality of a nearly inactive detergent-solubilized enzyme complex. (pasteur.fr)
  • A collection of reagents and other associated materials intended to be used for the qualitative and/or quantitative detection of one or multiple classes of antibodies to cardiolipin in a clinical specimen, using an enzyme immunoassay (EIA) method. (nih.gov)
  • Serum anti-GQ1b antibodies and IgM anti-cardiolipin antibody were positive. (alliedacademies.org)
  • Our case suggests that MFS presenting with proptosis and pain may be associated with serum anti-GQ1b antibodies and IgM anti-cardiolipin antibody. (alliedacademies.org)
  • described a Japanese MFS patient with low titer of serum anti-GQ1b antibodies and increased IgG anti-cardiolipin antibody [ 6 ]. (alliedacademies.org)
  • Here we report the first Chinese patient with MFS who showed proptosis and pain and had serum anti- GQ1b antibodies and IgM anti-cardiolipin antibody. (alliedacademies.org)
  • The BlueWell Cardiolipin IgG kit allows the quantitative detection of IgG antibodies to Cardiolipin in human serum. (ophthalmologymanagement.com)
  • The Cardiolipin Antibiotic Igm reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (gentaurprices.com)
  • Description: A sandwich ELISA for quantitative measurement of Human anti cardiolipin antibody IgM,ACA IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (gentaurprices.com)
  • Description: A sandwich ELISA for quantitative measurement of Mouse anti cardiolipin antibody IgM,ACA IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (gentaurprices.com)
  • The Anti-Cardiolipin IgM ELISA Assay is an indirect solid phase immunoassay kit for the quantitative measurement of IgM class auto-antibodies directed against Cardiolipina-β2-glycoprotein complex in human serum or plasma. (eaglebio.com)
  • The important role of cardiolipin in cardiovascular health is highlighted by several cardiac diseases, in which cardiolipin plays a fundamental role. (mpg.de)
  • Recently, there is a study on the enzymes that participate in cardiolipin biosynthesis and remodeling. (wikibooks.org)
  • After biosynthesis of cardiolipin, there are three remodeling enzymes that modify the acyl chain composition of cardiolipin, producing either a "dysfunctional" cardiolipin that is tied to michondrial dysfunction or a tissue-specific cardiolipin in its mature form. (wikibooks.org)
  • Cardiolipin biosynthesis takes place in the mitochondrion of eukaryotic cells. (wikibooks.org)
  • Barth syndrome, Sengers syndrome, and Dilated cardiomyopathy with ataxia (DCMA) are genetic disorders, which affect cardiolipin biosynthesis. (mpg.de)
  • Cardiolipin IgG or anticardiolipin IgG is a blood test done for detecting presence of antibodies in the blood that help in diagnosing antiphospholipid syndrome. (pharmeasy.in)
  • Anti-cardiolipin antibodies can be classified in two ways: As IgM, IgG or IgA As β2-glycoprotein dependent or independent In autoimmune disease, ACA are beta-2 glycoprotein dependent In syphilis, ACA are beta-2 glycoprotein independent and can be assayed using the Venereal Disease Research Laboratory test β2-glycoprotein I has been identified as Apolipoprotein H and is required for the recognition of ACA in autoimmune disease. (wikipedia.org)
  • Only a subset of autoimmune anti-cardiolipin antibodies bind Apo-H, these anti-apolipoprotein antibodies are associated with increased thrombosis. (wikipedia.org)
  • Also people with some autoimmune diseases such as SLE have cardiolipin antibodies in their blood. (ahealthyme.com)
  • Cardiolipin Antibodies are seen in a subgroup of patients with autoimmune disorders, particularly Systemic Lupus Erythematosus (SLE), who are at risk for vascular thrombosis, thrombocytopenia, cerebral infarct and/or recurrent spontaneous abortion. (specialtylabs.com)
  • Specifically, interaction of cardiolipin with cytochrome oxidase and adenine nucleotide translocase is required in order for both of these mitochondrial enzymes to maintain activity. (sigmaaldrich.com)
  • This is the accepted version of the following article:Characterization of the Cytochrome c Membrane-Binding Site Using Cardiolipin-Containing Bicelles with NMR, Hisashi Kobayashi,Satoshi Nagao, Shun Hirota, Angewandte Chemie, Oct 10, 2016, which has been published in final form at http://dx.doi.org/10.1002/anie.201607419. (naist.jp)
  • The localization of tightly bound cardiolipin in cytochrome ox. (mysciencework.com)
  • One to two molecules of tightly bound cardiolipin are associated with resolved fractions of cytochrome oxidase containing subunits I to III or I to IV. (mysciencework.com)
  • Large scale isolation of subunits I to IV indicates the presence of approximately 0.5 molecule of cardiolipin per molecule of subunit I. Lipoprotein staining of sodium dodecyl sulfate/urea/acrylamide gels of cytochrome oxidase support the findings that subunit I is a lipoprotein. (mysciencework.com)
  • Targeting mitochondrial cardiolipin and the cytochrome c/cardiolipin complex to promote electron transport and optimize mitochondrial ATP synthesis. (cornell.edu)
  • We identified a novel molecular signature of oncocytic tumors characterized by an abnormally high abundance and chemical diversity of cardiolipins (CL), including many oxidized species. (aacrjournals.org)
  • In other words, different organisms can have different final molecular forms of cardiolipin and even in different tissues in the same organism. (wikibooks.org)
  • Together, our results provide a molecular basis for the activation of a bacterial respiratory complex by cardiolipin. (pasteur.fr)
  • Here, we summarize molecular functions of cardiolipin in mitochondrial biogenesis and morphology. (mpg.de)
  • This test detects the presence of cardiolipin antibodies in the blood. (labtestsonline.org)
  • The identification of Barth′s syndrome, a disease associated with abnormalities in the fatty acid composition of cardiolipin causing cardiomyopathy and neutropenia, has displayed the importance of cardiolipin in mitochondrial functionality. (sigmaaldrich.com)
  • Anti-cardiolipin antibodies (ACA) are antibodies often directed against cardiolipin and found in several diseases, including syphilis, antiphospholipid syndrome, livedoid vasculitis, vertebrobasilar insufficiency, Behçet's syndrome, idiopathic spontaneous abortion, and systemic lupus erythematosus (SLE). (wikipedia.org)
  • Metabolomics Reveals New Mechanisms for Pathogenesis in Barth Syndrome and Introduces Novel Roles for Cardiolipin in Cellular Function. (kennedykrieger.org)
  • Barth Syndrome is the only known Mendelian disorder of cardiolipin remodeling, with characteristic clinical features of cardiomyopathy, skeletal myopathy, and neutropenia. (kennedykrieger.org)
  • Cardiolipin and monolysocardiolipin analysis in fibroblasts, lymphocytes, and tissues using high-performance liquid chromatography-mass spectrometry as a diagnostic test for Barth syndrome. (ru.nl)
  • Changes in cardiolipin concentrations and alterations to its acyl groups have led to a variety of pathological conditions such as heart failure. (sigmaaldrich.com)
  • Using cellular biology and quantitative biochemistry approaches, the goal of my research is to understand how changes in cardiolipin content lead cancer cells to resist drug treatment, specifically in glioblastoma multiforme, the most common and aggressive primary brain tumor. (calvin.edu)
  • Catalyzes the synthesis of cardiolipin (CL) (diphosphatidylglycerol) by specifically transferring a phosphatidyl group from CDP-diacylglycerol to phosphatidylglycerol (PG). (uniprot.org)
  • We found that the oxidation of cardiolipin leads to a conformational change both in the backbone/head group and in chain regions of oxidized cardiolipin molecules. (mendeley.com)
  • VALLEY COTTAGE, N.Y. - The Cardiolipin antibodies test market constitutes pathological services provides and kit manufacturers offering variant of products and services, the test kits are widely utilized in the diagnostic centers and hospital to have a rapid diagnosis of cardiolipin antibodies count in the body. (wn.com)
  • We demonstrated that siRNA ABCA1 knockdown in podocytes led to reduced oxygen consumption capabilities associated with alterations in the oxidative phosphorylation (OXPHOS) complexes and with cardiolipin accumulation. (jci.org)
  • How cardiolipin peroxidation alte. (mendeley.com)
  • Instead, cardiolipin mediates ABCA1-dependent susceptibility to podocyte injury, as inhibition of cardiolipin peroxidation with elamipretide improved DKD in vivo and prevented ABCA1-dependent podocyte injury in vitro and in vivo. (jci.org)
  • We demonstrated that this pathway is relevant to DKD and that ABCA1 inducers or inhibitors of cardiolipin peroxidation may each represent therapeutic strategies for the treatment of established DKD. (jci.org)
  • Radiolytic yield of cardiolipin peroxidation by gamma rays in large unilamellar vesicles of phosphatidylcholine. (semanticscholar.org)
  • Indeed, a gep4 null has small amounts of cardiolipin and phosphatidylglycerol, which build up phosphatidylglycerolphosphate and destabilized respiratory supercomplexes. (wikibooks.org)
  • A full screen for hyperviscosity conditions including plasma homocysteine levels, anti-DNA antibodies, cardiolipin antibodies, protein C and protein S, factor V Leiden and prothrombin 20210A was normal, as were the blood glucose level and a lipogram. (thefreedictionary.com)
  • The Anti Cardiolipin IgM ELISA Assay Kit is intended for research use only and not intended for diagnostic procedures. (eaglebio.com)
  • Eagle Biosciences Anti Cardiolipin Screen ELISA Assay Kit allows to determine the unknown concentration of auto-antibodies directed against the Cardiolipin-β2-glycoprotein complex through two different calibration curve (one specific for IgG test, one specific for IgM test), two different conjugates linked to horseradish peroxidase (one specific for IgG test, the other specific for IgM test) and one microplate only. (eaglebio.com)
  • This means the ImmunoWELL Caridiolipin kit reproducibly and simultaneously measures total cardiolipin and the more significant clinical marker, anti-beta-2 glycoprotein. (genbio.com)
  • With the demethylated c 8 -ring and with c 10 - and c 11 -rings, the density of bound cardiolipin molecules at this site increased, but residence times were not changed greatly. (pnas.org)
  • The resistance of this tightly bound cardiolipin to organic solvent extraction suggests a specific association of some tenacity with the protein. (mysciencework.com)
  • AMP-activated protein kinase alpha2 deficiency affects cardiac cardiolipin homeostasis and mitochondrial function. (inserm.fr)
  • 7. The composition of claim 1, wherein the cardiolipin is tetramyristoyl cardiolipin. (freepatentsonline.com)
  • In addition, the analysts of the report have served the qualitative and quantitative scrutinizing of different micro- and macro-economic factors influencing the global Cardiolipin Antibodies Testmarket. (pharmiweb.com)
  • ELISA method for quantitative determination of Anti-Cardiolipin / Anti-Phospholipid IgG, IgM, IgA isotypes. (aniara.com)
  • Diagnosis is based on thrombotic events and repeated positive tests for antiphospholipid antibodies, such as those against the phospholipid cardiolipin or the plasma protein betaz-glycoprotein I, as well as the lupus anticoagulant. (thefreedictionary.com)
  • The best characterized phospholipid-binding protein is beta-2 glycoprotein 1 (beta-2 GP1) and most immunoassays for phospholipid antibodies employ a composite substrate consisting of cardiolipin plus beta-2 GP1. (mayomedicallaboratories.com)
  • Structural details of an interaction between cardiolipin and an integral membrane protein. (semanticscholar.org)
  • Anti-cardiolipin antibody (ACA) is a type of antiphospholipid antibody (a blood protein) directed against the fat-like component of the mitochondrial cell membrane called cardiolipin. (fertilitysmarts.com)
  • Among these, the Cardiolipin is the most commonly used phospholipid as an antigen for determining the aPL by ELISA method. (eaglebio.com)
  • As there are four distinct alkyl chains in cardiolipin, the potential for complexity of this molecule species is enormous. (wikipedia.org)
  • However, in most animal tissues, cardiolipin contains 18-carbon fatty alkyl chains with 2 unsaturated bonds on each of them. (wikipedia.org)
  • Although the total amount of cardiolipin was unchanged, we disclosed in retinal samples increased amount of the major specie of cardiolipins, i.e. cardiolipins containing 18-carbon fatty alkyl chains with two unsaturated bonds on each of them (0.213±0.015 vs 0.405±0.13). (arvojournals.org)
  • The presence of cardiolipins with one or more i-C 25 alkyl chains, generally termed extended archaeol (EXT-AR), in one of the Methanonatronarchaeia strains was unexpected as only one other order of methanogenic archaea has been reported to produce EXT-AR. (frontiersin.org)
  • Mutation of LC3 residues predicted as cardiolipin-interaction sites by computational modelling inhibited its participation in mitophagy. (cdc.gov)
  • Our findings indicate that the Mic10 core machinery of MICOS is regulated by several mechanisms, including interaction with cardiolipin and antagonistic actions of Mic26 and Mic27. (rug.nl)
  • Cyt c is ordinarily anchored to the mitochondrial membrane through interaction with cardiolipin (CL), however its release into the cytosol initiates apoptosis. (naist.jp)
  • Finally, cardiolipin binding tunes the interaction with the quinol substrate. (pasteur.fr)
  • Overall, the results bring up that the conformation of cardiolipin is altered upon oxidation, suggesting that its oxidation may interfere its interactions with mitochondrial proteins and thereby affect cardiolipin-dependent cellular processes such as electron and proton transport. (mendeley.com)
  • So far, there are three known cardiolipin remodeling pathways as well as known proteins in the different stages of the cardiolipin biosynthetic pathways. (wikibooks.org)
  • Role of cardiolipin in stability of integral membrane proteins. (semanticscholar.org)
  • Together, these results expand our understanding of the numerous structural roles provided by cardiolipin for mitochondrial membrane proteins. (sciencemag.org)
  • Cardiolipin has been shown to perform a role in a number of other specific biological activities, including oxidative phosphorylation, apoptosis, cholesterol translocation and gene expression. (sigmaaldrich.com)
  • To determine whether cardiolipin is essential for yeast growth, we generated a cardiolipin synthase null mutant by disrupting the CLS1 gene (open reading frame YDL142c on chromosome IV) of Saccharomyces cerevisiae. (nih.gov)
  • In silico analysis of the M. hyorhinis genome revealed the absence of the pld gene but the presence of the cls gene encoding cardiolipin synthetase, which contains two PLD active domains. (asm.org)
  • The transformation of Mycoplasma gallisepticum that has neither the cls gene nor the reverse CAMP phenomenon with the cls gene from M. hyorhinis resulted in the reverse CAMP phenomenon, suggesting for the first time that reverse CAMP can be induced by cardiolipin synthetase. (asm.org)
  • Cardiolipin, a unique phospholipid composed of four fatty acid chains, is located mainly in the mitochondrial inner membrane (IM). (rupress.org)
  • The mAbs 2F5 and 4E10, two additional rare broadly reactive neutralizing mAbs (2G12 and IgG1b12) ( 11 - 13 ), and 31 common human mAbs to HIV-1 Env were tested for reactivity with cardiolipin ( 14 ) ( Table 1 ). (sciencemag.org)
  • Cardiolipin contains two phosphatidyl groups connected by a glycerol. (wikibooks.org)
  • Catalyzes the phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol. (uniprot.org)
  • 2 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) = a cardiolipin + glycerol. (uniprot.org)
  • A cardiolipin (CL) molecule is built of two glycerophosphate moieties, each carrying two fatty acid tails, which are connected via one more glycerol molecule (see Figure 1). (thefreelibrary.com)
  • Chemical synthesis of diphosphatidyl glycerol, a long-chain fatty acid ester of diphosphatidyl glycerol, phosphatidyl diglyceride and phosphatidyl glycerophosphate has stimulated a structural comparison with natural cardiolipin. (uu.nl)
  • A wide range of polar lipids were detected across the 13 strains including dialkyl glycerol diethers (archaeols), membrane-spanning glycerol tetraethers and diether-based cardiolipins. (frontiersin.org)
  • When an individual has inappropriate blood clot formation, recurrent miscarriages, cardiolipin antibodies, and/or another antiphospholipid antibody, the person may be diagnosed with antiphospholipid ayndrome (APS) . (labtestsonline.org)
  • Cardiolipin (CL) is a unique mitochondrial phospholipid, containing up to 85 wt% 18:2n-6 in mammals. (elsevier.com)
  • Cardiolipin is required for proper mitochondrial structure and function, and importantly, it facilitates the cellular events that initiate apoptosis. (calvin.edu)
  • Early Changes in Intramitochondrial Cardiolipin Distribution during Apoptosis -- Fernandez et al. (aacrjournals.org)
  • Cardiolipin and its different properties in mitophagy and apoptosis. (pubfacts.com)
  • A cardiolipin derivative in which all four of the phosphatidyl acyl groups are specified as myristoyl (tetradecanoyl). (zfin.org)
  • Disclosed herein is a composition comprising a liposome, a cardiolipin, and at least one antioxidant, wherein the composition is useful for improving, maintaining, or restoring a mitochondrial membrane and/or mitochondrial function. (freepatentsonline.com)
  • 1. A composition for improving, maintaining or restoring mitochondrial function comprising a liposome, a cardiolipin, and at least one antioxidant, wherein the cardiolipin is embedded in a phospholipid bilayer of the liposome and the at least one antioxidant is embedded in a phospholipid bilayer of the liposome, contained in an aqueous center of the liposome, or both. (freepatentsonline.com)
  • 3. The composition of claim 2, wherein the cardiolipin is tetraoleoyl-cardiolipin. (freepatentsonline.com)
  • 4. The composition of claim 3, wherein the at least one antioxidant is methylgentisate, and further wherein the methylgentisate is embedded in a phospholipid bilayer of the liposome along with the cardiolipin. (freepatentsonline.com)
  • 9. The composition of claim 1, wherein the cardiolipin is derived from a seed oil. (freepatentsonline.com)
  • Collectively, we describe a pathway definitively linking ABCA1 deficiency to cardiolipin-driven mitochondrial dysfunction. (jci.org)
  • Shotgun lipidomics identifies cardiolipin depletion in diabetic myocardium linking altered substrate utilization with mitochondrial dysfunction. (nih.gov)