A highly malignant, primitive form of carcinoma, probably of germinal cell or teratomatous derivation, usually arising in a gonad and rarely in other sites. It is rare in the female ovary, but in the male it accounts for 20% of all testicular tumors. (From Dorland, 27th ed & Holland et al., Cancer Medicine, 3d ed, p1595)
Neoplasms composed of primordial GERM CELLS of embryonic GONADS or of elements of the germ layers of the EMBRYO, MAMMALIAN. The concept does not refer to neoplasms located in the gonads or present in an embryo or FETUS.
A true neoplasm composed of a number of different types of tissue, none of which is native to the area in which it occurs. It is composed of tissues that are derived from three germinal layers, the endoderm, mesoderm, and ectoderm. They are classified histologically as mature (benign) or immature (malignant). (From DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p1642)
The malignant stem cells of TERATOCARCINOMAS, which resemble pluripotent stem cells of the BLASTOCYST INNER CELL MASS. The EC cells can be grown in vitro, and experimentally induced to differentiate. They are used as a model system for studying early embryonic cell differentiation.
Tumors or cancer of the TESTIS. Germ cell tumors (GERMINOMA) of the testis constitute 95% of all testicular neoplasms.
A malignant neoplasm of the germinal tissue of the GONADS; MEDIASTINUM; or pineal region. Germinomas are uniform in appearance, consisting of large, round cells with vesicular nuclei and clear or finely granular eosinophilic-staining cytoplasm. (Stedman, 265th ed; from DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, pp1642-3)
An unusual and aggressive tumor of germ-cell origin that reproduces the extraembryonic structures of the early embryo. It is the most common malignant germ cell tumor found in children. It is characterized by a labyrinthine glandular pattern of flat epithelial cells and rounded papillary processes with a central capillary (Schiller-Duval body). The tumor is rarely bilateral. Before the use of combination chemotherapy, the tumor was almost invariably fatal. (From DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p1189)
A malignant neoplasm made up of epithelial cells tending to infiltrate the surrounding tissues and give rise to metastases. It is a histological type of neoplasm but is often wrongly used as a synonym for "cancer." (From Dorland, 27th ed)
An important regulator of GENE EXPRESSION during growth and development, and in NEOPLASMS. Tretinoin, also known as retinoic acid and derived from maternal VITAMIN A, is essential for normal GROWTH; and EMBRYONIC DEVELOPMENT. An excess of tretinoin can be teratogenic. It is used in the treatment of PSORIASIS; ACNE VULGARIS; and several other SKIN DISEASES. It has also been approved for use in promyelocytic leukemia (LEUKEMIA, PROMYELOCYTIC, ACUTE).
A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)
The profession of writing. Also the identity of the writer as the creator of a literary production.
Collections of facts, assumptions, beliefs, and heuristics that are used in combination with databases to achieve desired results, such as a diagnosis, an interpretation, or a solution to a problem (From McGraw Hill Dictionary of Scientific and Technical Terms, 6th ed).
The guidelines and policy statements set forth by the editor(s) or editorial board of a publication.
Intentional falsification of scientific data by presentation of fraudulent or incomplete or uncorroborated findings as scientific fact.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
A rare tumor of the female genital tract, most often the ovary, formerly considered to be derived from mesonephric rests. Two varieties are recognized: (1) clear cell carcinoma, so called because of its histologic resemblance to renal cell carcinoma, and now considered to be of muellerian duct derivation and (2) an embryonal tumor (called also ENDODERMAL SINUS TUMOR and yolk sac tumor), occurring chiefly in children. The latter variety may also arise in the testis. (Dorland, 27th ed)
A malignant ovarian neoplasm, thought to be derived from primordial germ cells of the sexually undifferentiated embryonic gonad. It is the counterpart of the classical seminoma of the testis, to which it is both grossly and histologically identical. Dysgerminomas comprise 16% of all germ cell tumors but are rare before the age of 10, although nearly 50% occur before the age of 20. They are generally considered of low-grade malignancy but may spread if the tumor extends through its capsule and involves lymph nodes or blood vessels. (Dorland, 27th ed; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p1646)
The first alpha-globulins to appear in mammalian sera during FETAL DEVELOPMENT and the dominant serum proteins in early embryonic life.
The first of four extra-embryonic membranes to form during EMBRYOGENESIS. In REPTILES and BIRDS, it arises from endoderm and mesoderm to incorporate the EGG YOLK into the DIGESTIVE TRACT for nourishing the embryo. In placental MAMMALS, its nutritional function is vestigial; however, it is the source of INTESTINAL MUCOSA; BLOOD CELLS; and GERM CELLS. It is sometimes called the vitelline sac, which should not be confused with the VITELLINE MEMBRANE of the egg.
Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS.
A family of DNA-binding proteins that are primarily expressed in T-LYMPHOCYTES. They interact with BETA CATENIN and serve as transcriptional activators and repressors in a variety of developmental processes.
Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
A cell line derived from cultured tumor cells.
An organophosphate cholinesterase inhibitor that is used as a pesticide.
Enzymes which catalyze the hydrolysis of carboxylic acid esters with the formation of an alcohol and a carboxylic acid anion.
A mixture of isomeric tritolyl phosphates. Used in the sterilization of certain surgical instruments and in many industrial processes.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.
Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions.
The horn of an animal of the deer family, typically present only in the male. It differs from the HORNS of other animals in being a solid, generally branched bony outgrowth that is shed and renewed annually. The word antler comes from the Latin anteocularis, ante (before) + oculus (eye). (From Webster, 3d ed)
Proteins that are preferentially expressed or upregulated during FETAL DEVELOPMENT.
The middle germ layer of an embryo derived from three paired mesenchymal aggregates along the neural tube.
Euploid male germ cells of an early stage of SPERMATOGENESIS, derived from prespermatogonia. With the onset of puberty, spermatogonia at the basement membrane of the seminiferous tubule proliferate by mitotic then meiotic divisions and give rise to the haploid SPERMATOCYTES.
The reproductive cells in multicellular organisms at various stages during GAMETOGENESIS.
The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.
The process of germ cell development in the male from the primordial germ cells, through SPERMATOGONIA; SPERMATOCYTES; SPERMATIDS; to the mature haploid SPERMATOZOA.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
An enzyme that catalyzes the transfer of a methyl group from S-ADENOSYLMETHIONINE to the 5-position of CYTOSINE residues in DNA.
A condition of having no sperm present in the ejaculate (SEMEN).
Benign and malignant neoplastic processes that arise from or secondarily involve the brain, spinal cord, or meninges.
Therapeutic approach tailoring therapy for genetically defined subgroups of patients.
Component of the NATIONAL INSTITUTES OF HEALTH. Through basic and clinical biomedical research and training, it conducts and supports research with the objective of cancer prevention, early stage identification and elimination. This Institute was established in 1937.
A specified list of terms with a fixed and unalterable meaning, and from which a selection is made when CATALOGING; ABSTRACTING AND INDEXING; or searching BOOKS; JOURNALS AS TOPIC; and other documents. The control is intended to avoid the scattering of related subjects under different headings (SUBJECT HEADINGS). The list may be altered or extended only by the publisher or issuing agency. (From Harrod's Librarians' Glossary, 7th ed, p163)
The interaction of persons or groups of persons representing various nations in the pursuit of a common goal or interest.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form.
Established cell cultures that have the potential to propagate indefinitely.
Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed)
Scientific study of human skeletal remains with the express purpose of identification. This includes establishing individual identity, trauma analysis, facial reconstruction, photographic superimposition, determination of time interval since death, and crime-scene recovery. Forensic anthropologists do not certify cause of death but provide data to assist in determination of probable cause. This is a branch of the field of physical anthropology and qualified individuals are certified by the American Board of Forensic Anthropology. (From Am J Forensic Med Pathol 1992 Jun;13(2):146)
A cytoskeletal protein associated with cell-cell and cell-matrix interactions. The amino acid sequence of human vinculin has been determined. The protein consists of 1066 amino acid residues and its gene has been assigned to chromosome 10.
The nonstriated involuntary muscle tissue of blood vessels.
Non-striated, elongated, spindle-shaped cells found lining the digestive tract, uterus, and blood vessels. They are derived from specialized myoblasts (MYOBLASTS, SMOOTH MUSCLE).
The application of dental knowledge to questions of law.

Anticoagulant heparan sulfate precursor structures in F9 embryonal carcinoma cells. (1/312)

To understand the mechanisms that control anticoagulant heparan sulfate (HSact) biosynthesis, we previously showed that HSact production in the F9 system is determined by the abundance of 3-O-sulfotransferase-1 as well as the size of the HSact precursor pool. In this study, HSact precursor structures have been studied by characterizing [6-3H]GlcN metabolically labeled F9 HS tagged with 3-O-sulfates in vitro by 3'-phosphoadenosine 5'-phospho-35S and purified 3-O-sulfotransferase-1. This later in vitro labeling allows the regions of HS destined to become the antithrombin (AT)-binding sites to be tagged for subsequent structural studies. It was shown that six 3-O-sulfation sites exist per HSact precursor chain. At least five out of six 3-O-sulfate-tagged oligosaccharides in HSact precursors bind AT, whereas none of 3-O-sulfate-tagged oligosaccharides from HSinact precursors bind AT. When treated with low pH nitrous or heparitinase, 3-O-sulfate-tagged HSact and HSinact precursors exhibit clearly different structural features. 3-O-Sulfate-tagged HSact hexasaccharides were AT affinity purified and sequenced by chemical and enzymatic degradations. The 3-O-sulfate-tagged HSact hexasaccharides exhibited the following structures, DeltaUA-[6-3H]GlcNAc6S-GlcUA-[6-3H]GlcNS3(35)S+/-6S-++ +IdceA2S-[6-3H]Glc NS6S. The underlined 6- and 3-O-sulfates constitute the most critical groups for AT binding in view of the fact that the precursor hexasaccharides possess all the elements for AT binding except for the 3-O-sulfate moiety. The presence of five potential AT-binding precursor hexasaccharides in all HSact precursor chains demonstrates for the first time the processive assembly of specific sequence in HS. The difference in structures around potential 3-O-sulfate acceptor sites in HSact and HSinact precursors suggests that these precursors might be generated by different concerted assembly mechanisms in the same cell. This study permits us to understand better the nature of the HS biosynthetic pathway that leads to the generation of specific saccharide sequences.  (+info)

Differential expression of mouse Disabled 2 gene in retinoic acid-treated F9 embryonal carcinoma cells and early mouse embryos. (2/312)

Using a differential display PCR, we identified a differentially expressed cDNA fragment which was detectable in retinoic acid (RA) treated F9 embryonal carcinoma (EC) cells but not in untreated F9 cells. A homology search of the Gene Bank indicated that the cDNA fragment is part of the mouse homolog of the Drosophila Disabled (mDab2) gene. Aggregate cultures of F9 EC cells grown in the presence of the RA differentiated into nonmalignant cells resembling the visceral endoderm of the mouse embryo. Upon induction of endodermal differentiation with 10(-7) M RA, the gene expression of mDab2 was increased gradually during the first 96 h. Neither undifferentiated F9 cells, nor the undifferentiated aggregate cells without RA expressed mDab2. Whole-mount in situ hybridization and quantitative RT-PCR also showed that the temporal expression pattern of the mDab2 gene coincides with the initiation pattern of RA synthesis that occurs during mouse embryogenesis. Also, two alternative splicing messages of mDab2 were detected in a tissue specific manner. All the data indicate that mDab2 may play an important role in RA-induced signal transduction during mouse development.  (+info)

Tyrosine phosphorylation of C-Cbl facilitates adhesion and spreading while suppressing anchorage-independent growth of V-Abl-transformed NIH3T3 fibroblasts. (3/312)

The protooncogenic protein c-Cbl becomes tyrosine phosphorylated in normal cells in response to a variety of external stimuli, as well as in cells transformed by oncogenic protein tyrosine kinases. Tyrosine phosphorylation of c-Cbl upregulates its binding to multiple crucial signaling molecules. However, the biological consequences of c-Cbl-mediated signaling are insufficiently understood. To analyse the biological functions of c-Cbl, we overexpressed wild-type c-Cbl and its tyrosine phosphorylation-defective mutant form in v-Abl-transformed NIH3T3 fibroblasts. In this system, wild-type c-Cbl facilitated adhesion and spreading of v-Abl-transformed fibroblasts on the extracellular matrix, while reducing anchorage independence of these cells, as measured by their colony-forming efficiency in soft agar. Therefore, overexpression of wild-type c-Cbl exhibits an overall transformation-suppressing effect. By contrast, overexpression of a tyrosine phosphorylation-defective form of c-Cbl increases neither adhesion nor anchorage dependence of v-Abl-transformed fibroblasts. Analysis of the role of individual tyrosine phosphorylation sites of c-Cbl in these phenomena indicates that both phosphatidylinositol-3' kinase and the CrkL adaptor protein may be involved in the observed effects of c-Cbl. To summarize, the results presented in this report indicate that c-Cbl is involved in regulation of cell adhesion and cytoskeletal rearrangements, and that these effects of c-Cbl are dependent on its tyrosine phosphorylation.  (+info)

Retinoic acid promotes ubiquitination and proteolysis of cyclin D1 during induced tumor cell differentiation. (4/312)

Mechanisms by which differentiation programs engage the cell cycle are poorly understood. This study demonstrates that retinoids promote ubiquitination and degradation of cyclin D1 during retinoid-induced differentiation of human embryonal carcinoma cells. In response to all-trans-retinoic acid (RA) treatment, the human embryonal carcinoma cell line NT2/D1 exhibits a progressive decline in cyclin D1 expression beginning when the cells are committed to differentiate, but before onset of terminal neuronal differentiation. The decrease in cyclin D1 protein is tightly associated with the accumulation of hypophosphorylated forms of the retinoblastoma protein and G(1) arrest. In contrast, retinoic acid receptor gamma-deficient NT2/D1-R1 cells do not growth-arrest or accumulate in G(1) and have persistent cyclin D1 overexpression despite RA treatment. Notably, stable transfection of retinoic acid receptor gamma restores RA-mediated growth suppression and differentiation to NT2/D1-R1 cells and restores the decline of cyclin D1. The proteasome inhibitor LLnL blocks this RA-mediated decline in cyclin D1. RA treatment markedly accelerates ubiquitination of wild-type cyclin D1, but not a cyclin D1 (T286A) mutant. Transient expression of cyclin D1 (T286A) in NT2/D1 cells blocks RA-mediated transcriptional decline of a differentiation-sensitive reporter plasmid and represses induction of immunophenotypic neuronal markers. Taken together, these findings strongly implicate RA-mediated degradation of cyclin D1 as a means of coupling induced differentiation and cell cycle control of human embryonal carcinoma cells.  (+info)

The role of chemotherapy in intracranial germinoma: a case report. (5/312)

BACKGROUND: The case of a 29-year-old man with histologically proven simultaneous germinoma (seminoma) of the pineal gland and a stage I embryonal carcinoma of the testis is reported. An intradural metastatic lesion from the pineal germinoma was diagnosed at the level of the first thoracic vertebra. Treatment, after inguinal orchiectomy, was chemotherapy only, rather than conventional radiotherapy for the pineal germinoma. METHODS: Therapy consisted of bleomycin (B), etoposide (E) and cisplatin (P). MRI was used to assess the effectiveness of BEP chemotherapy. RESULTS: A complete remission of the pineal gland germinoma and the epidural metastasis was documented after two cycles of BEP chemotherapy and after 15 months of follow-up the patient remains free of relapse. DISCUSSION: The pathogenesis of simultaneously occurring germinoma of the pineal gland and embryonal cell carcinoma of the testis is discussed. The choice of therapy in these circumstances is a matter of debate and the good result of chemotherapy alone in this patient suggest that primary chemotherapy may be the therapy of choice in patients with pineal germinomas.  (+info)

Interdependent action of RalGEF and Erk in Ras-induced primitive endoderm differentiation of F9 embryonal carcinoma cells. (6/312)

Previous work by us and others has implicated a role for Ral guanine exchange factors (RalGEFs) in Ras-induced cell growth and oncogenic transformation. Here we show for the first time that RalGEFs are involved in Ras-induced differentiation as well. Expression of oncogenic Ras in F9 embryonal carcinoma (EC) cells is known to induce differentiation to a primitive endoderm (PrE)-like phenotype, but the downstream signal transduction mechanisms involved are unclear. We found that PrE differentiation is induced by the Ras effector domain mutants, RasV12G37 and RasV12E38, but not by RasV12C40. Accordingly, expression of constitutively active forms of RalGEF (Rlf-CAAX) or Rafl (Raf-CAAX) is sufficient to induce differentiation. Inhibition of RalGEF activity by expression of dominant negative Ral completely abolishes Rlf-CAAX- and RasV12G37-induced differentiation, while it reduces differentiation by RasV12 and Raf-CAAX. Finally, while Rlf-CAAX does not increase Erk activity, inhibition of MEK blocks both Ras- as well as Rlf-CAAX-induced differentiation, suggesting that RalGEFs induce PrE differentiation in a manner depending on basal MEK or Erk activity. Based on these results we conclude that Ras induces PrE differentiation of F9 EC cells via an interplay of Erk-and RalGEF-mediated pathways.  (+info)

Volume of liquid below the epithelium of an F9 cell as a signal for differentiation into visceral endoderm. (7/312)

When retinoic acid-primed F9 cells are allowed to aggregate, they form embryoid bodies with an outer layer of (&agr;)-fetoprotein-producing visceral endoderm cells and an internal cavity. I show that maturation of the visceral endoderm is dependent on the size of F9 aggregates. Size fractionation of aggregates of retinoic acid-primed F9 cells on Percoll density gradients revealed that only aggregates with diameters larger than 180 microm developed into embryoid bodies with an endoderm layer secreting (&agr;)-fetoprotein. Size dependent alpha-fetoprotein-secretion was also observed when retinoic acid-primed F9 cells were cultured on porous microcarrier beads larger than 185 microm. Retinoic acid-primed F9 cells on flat microporous membranes did not differentiate and secrete alpha-fetoprotein unless exposed to a limited volume of medium at their basolateral surface. This suggested that maturation of the visceral endoderm is signaled by the volume of liquid phase below the epithelium. I postulate that the epithelial layer of an F9 aggregate encloses liquid and forms a barrier to diffusion of some critical factor(s). The concentration of such a factor may reach a threshold due to enlargement of the liquid phase during growth of the F9 aggregate and thereby signal maturation of the outer layer of cells into visceral endoderm.  (+info)

Alternative promoters direct tissue-specific expression of the mouse protein phosphatase 2Cbeta gene. (8/312)

Type 2C protein phosphatases (PP2Cs), a class of ubiquitous and evolutionally conserved serine/threonine protein phosphatases, are encoded in at least four distinct genes and implicated in the regulation of various cellular functions. Of these four PP2C genes, the expression of the PP2Cbeta gene has been reported to be tissue-specific and development-dependent. To understand more precisely the regulatory mechanism of this expression, we have isolated and characterized overlapping mouse genomic lambda clones. A comparison of genomic sequences with PP2Cbeta cDNA sequences provided information on the structure and localization of intron/exon boundaries and indicated that PP2Cbeta isoforms with different 5' termini were generated by alternative splicing of its pre-mRNA. The 5'-flanking region of exon 1 had features characteristic of a housekeeping gene: it was GC-rich, lacked TATA boxes and CAAT boxes in the standard positions, and contained potential binding sites for the transcription factor SP1. In the 5'-flanking region of exon 2, several consensus sequences were found, such as a TATA-like sequence and negative regulatory element box-1, -2 and -3. Subsequent analysis by transient transfection assay with a reporter gene showed that these regions act as distinct promoters. Analysis of PP2Cbeta transcripts by reverse transcriptase-PCR showed that exon-1 transcripts were expressed ubiquitously in all of the tissues examined, whereas exon-2 transcripts were predominantly expressed in the testis, intestine and liver. These results suggest that the alternative usage of two promoters within the PP2Cbeta gene regulates tissue-specific expression of PP2Cbeta mRNA.  (+info)

Embryonic stem cells and embryonal carcinoma P19 cells produce erythropoietin (Epo) in an oxygen-independent manner, although lactate dehydrogenase A (LDHA) is hypoxia-inducible. To explore this paradox, we studied the operation of cis-acting sequences from these genes in P19 and Hep3B cells. The Epo gene promoter and 3 enhancer from P19 cells conveyed hypoxia-inducible responses in Hep3B cells but not in P19 cells. Together with DNA sequencing and the normal transcription start site of P19 Epo gene, this excluded the possibility that the noninducibility of Epo gene in P19 cells was due to mutation in these sequences or unusual initiation of transcription. In contrast, reporter constructs containing LDHA enhancer and promoter were hypoxia inducible in P19 and Hep3B cells, and mutation of a hypoxia- inducible factor 1 (HIF-1) binding site abolished the hypoxic inducibility in both cells, indicating that HIF-1 activation operates normally in P19 cells. Neither forced expression of hepatocyte nuclear
TY - JOUR. T1 - Apoptosis of human embryonal carcinoma cells with in vitro differentiation. AU - Yamada, Taketo. AU - Suzuki, Nao. AU - Hiraoka, Nobuyoshi. AU - Matsuoka, Kentaroh. AU - Fukushima, Sachiko. AU - Hashiguchi, Akinori. AU - Hata, Jun Ichi. PY - 1996/2. Y1 - 1996/2. N2 - An in vitro model of apoptosis and differentiation in human embryonal carcinoma (EC) cells was developed to study the mode of cell death and mechanisms of cell death in early development. Death of these cells was induced by treatment with retinoic acid (RA) under the same conditions as those for induction of differentiation. The manner of this cell death was apoptosis, not necrosis, with the morphological criterion for apoptosis. Serum deprivation likewise caused apoptosis in both undifferentiated and differentiated EC cells. In differentiated EC cells, DNA fragmentation was observed in a smear pattern lacking the ladder pattern typically associated with apoptosis. However, in differentiated EC cells, DNA ...
The lamin complement of nuclear matrix isolated from F9 embryonal carcinoma cells was studied during retinoic acid-induced differentiation in culture. Differentiation of the original cells into parietal endoderm-like cells was accompanied by the gradual appearance of lamins A and C while lamin B was present throughout all stages. Lamins were identified by their molecular masses, isoelectric points, recognition by a monoclonal antibody and a polyclonal antiserum, and by peptide mapping. The increase in the amounts of lamins A and C found in the matrix was due to de novo synthesis as no extranuclear pools of these lamins were detected in the undifferentiated cells. These results provide biochemical evidence that, as in amphibian embryogenesis, there are variations in nuclear lamina composition during mammalian development. ...
Adult human male germ cell tumors are unique in their display of histopathologies that resemble different stages of human development, and thus comprise a model system for the study of molecular mechanisms involved in human ES3 cell development (1) . Cell lines derived from such tumors, in particular, EC, have provided an invaluable in vitro resource in which molecular events regulating cell fate/lineage decision can be studied (2) . The EC cell lines can be maintained in an undifferentiated state in vitro, and undergo spontaneous or morphogen-induced differentiation programs. Some display the ability to differentiate along both somatic and extra-embryonic lineages placing them as equivalents of cells in the inner cell mass of the developing embryo (3 , 4) , whereas others exhibit a more restricted pluripotential differentiation program (5 , 6) . Global perspectives of the expression patterns of such cell lines during different differentiation programs may lead to the identification of genes ...
Two distinct murine heat shock transcription factors, HSF1 and HSF2, have been identified. HSF1 mediates the transcriptional activation of heat shock genes in response to environmental stress, while the function of HSF2 is not understood. Both factors can bind to heat shock elements (HSEs) but are maintained in a non-DNA-binding state under normal growth conditions. Mouse embryonal carcinoma (EC) cells are the only mammalian cells known to exhibit HSE-binding activity, as determined by gel shift assays, even when maintained at normal physiological temperatures. We demonstrate here that the constitutive HSE-binding activity present in F9 and PCC4.aza.R1 EC cells, as well as a similar activity found to be present in mouse embryonic stem cells, is composed predominantly of HSF2. HSF2 in F9 EC cells is trimerized and is present at higher levels than in a variety of nonembryonal cell lines, suggesting a correlation of these properties with constitutive HSE-binding activity. Surprisingly, ...
Substantial multiplication in vitro of cloned cells from a human embryonal carcinoma cell line, Tera 2, has been obtained in a basal medium (DMEM/Hams F12,50:50, v/v) supplemented with 10 micrograms low density lipoprotein/ml, 100 micrograms high density lipoprotein/ml, 100 ng multiplication stimulating activity/ml, 100 ng insulin/ml and 1 microgram transferrin/ml. The growth rate appears to be similar to that obtained in 10% serum. Furthermore, studies on the expression of cell surface receptors revealed that cloned Tera 2 cells express high-affinity receptors for IGF-II but not for insulin. The cells also express receptors for Epidermal Growth Factor (EGF) even though the addition of EGF does not stimulate their proliferation in serum-free medium. These results suggest that the expression of specific growth factor receptors is not an absolute determinant of hormone responsiveness. ...
TY - JOUR. T1 - Disabled-2 Mediates c-Fos Suppression and the Cell Growth Regulatory Activity of Retinoic Acid in Embryonic Carcinoma Cells. AU - Smith, Elizabeth R.. AU - Capo-Chichi, Callinice D.. AU - He, Junqi. AU - Smedberg, Jennifer L.. AU - Yang, Dong Hua. AU - Prowse, Amanda H.. AU - Godwin, Andrew K.. AU - Hamilton, Thomas C.. AU - Xu, Xiang Xi. PY - 2001/12/14. Y1 - 2001/12/14. N2 - F9 embryonic stem cell-like teratocarcinoma cells are widely used to study early embryonic development and cell differentiation. The cells can be induced by retinoic acid to undergo endodermal differentiation. The retinoic acid-induced differentiation accompanies cell growth suppression, and thus, F9 cells are also often used as a model for analysis of retinoic acid biological activity. We have recently shown that MAPK activation and c-Fos expression are uncoupled in F9 cells upon retinoic acid-induced endodermal differentiation. The expression of the candidate tumor suppressor Disabled-2 is induced and ...
Changes to cell cycle-regulating machinery that occur during differentiation of cells are thought to be responsible mostly for withdrawal from cycling. Here, embryonal carcinoma (EC) cell lines were found that differ in their basal levels of p27 inhibitor of cyclin-dependent kinases but not in their growth rates, distribution of cells in phases of cell cycle, and their ability to differentiate. High basal levels of p27 did not substitute for up-regulation of p27 that in EC cells normally occurs early after entering a differentiation pathway. Under both standard and differentiation-supporting culture conditions, variances in the levels of p27 were strictly followed by variances in the levels of cyclins D2 and D3. In EC cells genetically manipulated to overexpress p27 protein, cyclin D3 became up-regulated and vice versa. Supposedly, titration of p27 by D-type cyclins, which prevents its inhibitory action toward cyclin-dependent kinase 2, allows for the maintenance of elevated p27 in proliferating ...
Overall survival of 425 advanced renal-cell carcinoma patients treated with outpatient s.c. IL-2/INF-α2a therapy (A). Overall survival of 163 low-risk patients
Mcburney, M W. and Strutt, B, Fusion of embryonal carcinoma cells to fibroblast cells, cytoplasts, and karyoplasts. (1979). Subject Strain Bibliography 1979. 1089 ...
An embryonal carcinoma is a malignant growth that develops in germ cells. Common types of embryonal carcinomas include teratomas...
TY - JOUR. T1 - Understanding the roles of growth factors in carcinogenesis. T2 - Modulation of autocrine growth control by differentiation. AU - Rizzino, A.. PY - 1993. Y1 - 1993. N2 - It is widely believed that abnormal proliferation of tumor cells is due, at least in part, to the production of autocrine growth factors that are not produced by their normal counterparts. However, direct support for this belief is seriously lacking. The normal counterparts of the vast majority of tumor cells have not been identified adequately and, thus, the growth factors produced by the normal counterparts of tumor cells have not been described. This review summarizes the remarkable similarity in the types of growth factors and growth factor receptors produced by early mouse embryos and by mouse embryonal carcinoma cells, the stem cells of teratocarcinomas. Based on these similarities and the likelihood that embryonal carcinoma cells are derived from the totipotent cells of the mammalian embryo, it is argued ...
Embryonal carcinoma is a germ cell tumor characterized by primitive epithelial cells with marked pleomorphism and various histologic patterns. It may present in pure form but often is part of a mixed germ cell tumor.
T-cell factor 3 (TCF3), a downstream effector of Wnt signaling in embryonic stem (ES) cells, plays an important role in pluripotent self-renewal and proliferation. Loss of TCF3 delays the differentiation of mouse ES cells. The purpose of this study was to investigate the effect of TCF3 on embryonal carcinoma (EC). The mouse F9 EC cell line and a tumor-bearing mouse model were used to evaluate the anti-EC tumor effects of TCF3 in vitro and in vivo, respectively. The overexpression of TCF3 significantly inhibited proliferation, colony-forming and migration in F9 EC cells by approximately 30, 45 and 30%, respectively. The in vivo mouse model showed that the overexpression of TCF3 significantly reduced tumor volume (36.4%) and tumor weight (34.8%), malignancy progression and local infiltration and prolonged the life span of tumor-bearing mice. Overexpression of TCF3 significantly down-regulated Oct4 expression in the F9 EC cells. The results indicate that TCF3 is an inhibitor of the malignant ...
Oshima, R, Stimulation of the clonal growth and differentiation of feeder layer dependent mouse embryonal carcinoma cells by beta-mercaptoethanol. (1978). Subject Strain Bibliography 1978. 2177 ...
Cell lines. Human AML lines carrying FLT3ITD mutations were kindly donated by Yoshinobu Matsuo (MOLM-13, Fujisaki Cell Center, Hayashibara Biochemical Labs, and the Kurashiki Medical Center, Kurashiki, Okayama, Japan), Neill Giese (MOLM-14; Calistoga Pharmaceuticals, Inc.), Stefan Heinrichs (MV4;11, Department of Pediatric Oncology, Farber Cancer Institute, Boston, Massachusetts, USA), and David Sternberg (Ba/F3-FLT3ITD, OSI Pharmaceuticals). MOLM-13, MOLM-14 (50), and MV4;11 (CRL 9591; ATCC) as well as U937 (CRL 1593; ATCC) were grown in RPMI 1640 with 10% FBS. The murine bone marrow-derived IL-3-dependent Ba/F3 cell line (51) was cultured in RPMI with 10% FBS and 10% WEHI-3B conditioned medium. A Ba/F3 stable line expressing the FLT3ITD mutant N51 (24) was maintained in RPMI/10% FBS/10% WEHI-3B conditioned medium and 750 μg/ml active G418. Human Embryonal Carcinoma 293T (HEK 293T; CRL 11268, ATCC) and Phoenix-Ampho Packaging (Orbigen) cell lines were cultured in DMEM with 10% FBS. Patient ...
Neuronal and mesodermal differentiation of P19 embryonal carcinoma cells is characterized by expression of specific marker genes and modulated by activin and fibroblast growth factors ...
Historically, only few chemicals have been identified as neurodevelopmental toxicants, however, concern remains, and has recently increased, based upon the association between chemical exposures and increased developmental disorders. Diminution in motor speed and latency has been reported in preschool children from agricultural communities. Organophosphorus compounds (OPs) are pesticides due to their acute insecticidal effects mediated by the inhibition of acetylcholinesterase, although other esterases as neuropathy target esterase (NTE) can also be inhibited. Other neurological and neurodevelopmental toxic effects with unknown targets have been reported after chronic exposure to OPs in vivo. We studied the initial stages of retinoic acid acid-triggered differentiation of pluripotent cells towards neural progenitors derived from human embryonal carcinoma stem cells to determine if neuropathic OP, mipafox, and non-neuropathic OP, paraoxon, are able to alter differentiation of neural precursor cells in
The expression of the E2A transcription unit of the adenovirus E1A deletion mutant d 312 in murine embryonal carcinoma (EC) stem cells suggests that they contain an activity that will complement viral E1A. We have prepared from these cells in vitro transcription extracts that use E1A-inducible promoters more efficiently than do extracts from a differentiated cell line. Mixing experiments demonstrate that the EC phenotype is dominant. Gel retardation assays using the E2A promoter detect a binding activity present in F9 and PCC4 EC cells but not in differentiated cells. Our data indicate that EC stem cells contain a transcription factor that is analogous to viral E1A and is likely to be involved in the control of cellular gene expression during differentiation. © 1987.
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Genome wide profiling of human embryonic stem cells (hescs), their derivatives and embryonal carcinoma cells to develop base profiles of u.S. Federal government approved hesc lines ...
TY - JOUR. T1 - Characterization of a murine cellular SV40 T antigen in SV40-transformed cells and uninfected embryonal carcinoma cells. AU - Linzer, D. I H. AU - Maltzman, W.. AU - Levine, A. J.. PY - 1979/12/1. Y1 - 1979/12/1. UR - http://www.scopus.com/inward/record.url?scp=0018692275&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0018692275&partnerID=8YFLogxK. M3 - Article. C2 - 6253135. AN - SCOPUS:0018692275. VL - 44. SP - 215. EP - 224. JO - Cold Spring Harbor Symposia on Quantitative Biology. JF - Cold Spring Harbor Symposia on Quantitative Biology. SN - 0091-7451. IS - 1. ER - ...
INTRODUCTION Cancer of the testes is currently the most frequent neoplasm and a leading cause of morbidity in men 15-35 years of age. Its incidence is increasing. Embryonal carcinoma is its most malignant form, which either may be resistant or may develop resistance to therapies, which results in relapses. Cancer stem cells are hypothesized to be drivers of these phenomena. SPECIFIC AIM The specific aim of this work was identification and isolation of spectra of single, living cancer stem cells, which were acquired directly from the patients biopsies, followed by testing of their pluripotency. PATIENTS METHODS Biopsies were obtained from the patients with the clinical and histological diagnoses of the primary, pure embryonal carcinomas of the testes. The magnetic and fluorescent antibodies were genetically engineered. The SSEA-4 and TRA-1-60 cell surface display was analyzed by multiphoton fluorescence spectroscopy (MPFS), flow cytometry (FCM), immunoblotting (IB), nuclear magnetic resonance
A heparin binding mitogenic protein isolated from bovine uterus shares NH2-terminal amino acid sequence with a protein isolated from newborn rat brain. The cDNAs of the bovine, human, and rat genes have been isolated and encode extraordinarily conserved proteins unrelated to known growth or neurotrophic factors, although identity of nearly 50 percent has been found with the predicted sequence of a retinoic acid induced transcript in differentiating mouse embryonal carcinoma cells. Lysates of COS-7 cells transiently expressing this protein were mitogenic for NRK cells and initiated neurite outgrowth from mixed cultures of embryonic rat brain cells. RNA transcripts encoding this protein were widely distributed in tissues and were developmentally regulated. This protein, previously designated as heparin binding growth factor (HBGF)-8, is now renamed pleiotrophin (PTN) to reflect its diverse activities. PTN may be the first member of a family of developmentally regulated cytokines.
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Pao, P.C., Huang, N.K., Liu, Y.W., Yeh, S.H., Lin, S.T., Hsieh, C.P., Huang, A.M., Huang, H.S., Tseng, J.T., Chang, W.C., Lee, Y.C. (2011) A novel RING finger protein, Znf179, modulates cell cycle exit and neuronal differentiation of P19 embryonal carcinoma cells. Cell Death and Differentiation 18:1791-1804 ...
Current clinical judgment in bladder cancer (BC) relies primarily on pathological stage and grade. We investigated whether a molecular classification of tumor cell differentiation, based on a developmental biology approach, can provide additional prognostic information. Exploiting large preexisting gene-expression databases, we developed a biologically supervised computational model to predict markers that correspond with BC differentiation. To provide mechanistic insight, we assessed relative tumorigenicity and differentiation potential via xenotransplantation. We then correlated the prognostic utility of the identified markers to outcomes within gene expression and formalin-fixed paraffin-embedded (FFPE) tissue datasets. Our data indicate that BC can be subclassified into three subtypes, on the basis of their differentiation states: basal, intermediate, and differentiated, where only the most primitive tumor cell subpopulation within each subtype is capable of generating xenograft tumors and ...
Data Availability StatementThe datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request. human EC cell line Eca109. The level of cell radiosensitivity was determined by colony formation assay, and the localization of Gli1 was detected using immunofluorescence. Western blotting was used to determine the protein expression levels of Gli1, Shh, patched 1 (Ptch) and smoothened frizzled class receptor (Smo) in the two cell lines. Significantly higher levels of Gli1 were identified in the Eca109R cell line compared with those inEca109 cells (P 0.05). Additionally, western blotting analysis exhibited an increased expression level of the Gli1, Shh, Ptch and Smo proteins in Eca109R, compared with Eca109 cells (P 0.05). Overexpression of Gli1 in the parental cell line led to decreased levels of radiosensitivity and radiosensitivity of the radioresistant cell line was restored through knockdown of Gli1. The present study exhibited that Gli1 ...
Detailed information about the celline expression of FKBP5 in NTERA-2 stained with HPA031092. The antibody showed a High level of staining
Boer B, KNopp J, Mallanna S, Desler M, Chakravarthy H, Wilder PJ, Bernadt C, Rizzino A.Elevating the levels of Sox2 in embryonal carcinoma cells and embryonic stem cells inhibits the expression of Sox2:Oct-3/4 target genes. Nucleic Acids Res 2007 35(6):1773-1786 Abstract ...
Tumorigenesis is often described while a result of accumulated mutations that business lead to development benefit and clonal growth of mutated cells. mouse-human cell blend. To our understanding this is usually the 1st proof that human being malignancy cells of embryonic or somatic roots react to developing indicators produced by the mouse mammary gland microenvironment during gland regeneration by intramuscular shots of the computer virus in the wings of hatchling hens [6]. Fast developing tumors created in nearly 100% of the shot hens [6]. Nevertheless, when the labeled pp60src computer virus was utilized to infect poultry arm or leg embryo cells in 1975 exhibited that the blastocyst was capable to suppress tumorigenesis just when the quantity of embryonal carcinoma cells was lower than 20 cells per shot [19,20]. Further 211555-04-3 manufacture research by McCullough and collagues recommended that adjustments that affect a growth nonpermissive microenvironment framework and/or signaling such ...
TY - JOUR. T1 - Differentiation of mouse P19 embryonic carcinoma stem cells injected into an empty zebrafish egg chorion in a microfluidic device. AU - Lee, Jin Woo. AU - Na, Dae Seok. AU - Kang, Ji Yoon. AU - Lee, Sang Ho. AU - Ju, Byeong Kwon. N1 - Funding Information: This research was supported by the Intelligent Micro-system Center (IMC, http://www.microsystem.re.kr), which is carrying out one of the 21st Centurys Frontier R&D Projects sponsored by the Korea Ministry of Commerce, Industry, and Energy. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2006. Y1 - 2006. N2 - Mouse P19 embryonic carcinoma (EC) stem cells were xenotransplanted into the emptied chorion, the transparent envelope of a fertilized zebrafish egg (rather than mouse native zona pellucida) combined with a microfluidic device to study P19 EC cell differentiation in the chorion biomaterial. A distilled-water jet was used to remove the innate yolk and perivitelline inner mass from the chorion. P19 EC ...
The retinoids exert potent growth and differentiation effects on normal, embryonic and neoplastic cells. Although retinoids are known to regulate gene transcription through activation of retinoid receptors, the direct target genes of retinoid receptors remain largely unknown. A human embryonal carcinoma (EC) model was employed to determine the importance of p53 in retinoid-mediated differentiation and signaling. The hypothesis was that one mechanism by which retinoids regulate gene expression and mediate beneficial anti-tumor effects is by activation of p53 transactivation activity. RA was found to enhance the transactivation function of p53 in the human EC line NT2/D1. A derived RA-resistant line, NT2/D1-R1, was deficient in this activity, and also exhibited cross-resistance to cisplatin. This suggested a model in which RA and cisplatin signaling pathways impinge upon p53 in human EC cells. Further evidence generated to support this model is the following: (1) RA-induced p53 activity could be ...
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PRAD-1 is a putative oncogene localized on chromosome 11q13 which encodes cyclin D1, a novel cyclin involved in cell cycle regulation. Amplification of this gene has recently been reported in several human tumors including breast and head and neck carcinomas. In this study we have analyzed the presence of PRAD-1/cyclin D1 gene amplification and mRNA overexpression in a series of 46 matched normal mucosas and squamous cell carcinomas of the larynx. PRAD-1/cyclin D1 was found to be amplified 2- to 12-fold in 17 carcinomas (37%). DNA amplification correlated with advanced local invasion (P = 0.0015), presence of lymph node metastases (P = 0.0078), and stage IV of the tumors (P = 0.0021). mRNA overexpression was found in 15 of the 43 (35%) cases examined and it was also significantly associated with advanced local invasion (P = 0.0025) and stage IV carcinomas (P = 0.0032). A significant association was observed between gene amplification and mRNA overexpression (P , 0.0001) with only 3 discordant ...
Vertebrate Hox and Otx genes encode homeodomain-containing transcription factors thought to transduce positional information along the body axis in the segmental portion of the trunk and in the rostral brain, respectively. Moreover, Hox and Otx2 genes show a complementary spatial regulation during embryogenesis. A 1821-base pair (bp) upstream DNA fragment of the Otx2 gene is positively regulated by co-transfection with expression vectors for the human HOXB1, HOXB2, and HOXB3 proteins in an embryonal carcinoma cell line (NT2/D1) and a shorter fragment of only 534 bp is able to drive this regulation. The HOXB1, HOXB2, and HOXB3 DNA-binding region on the 534-bp Otx2 genomic fragment has been demonstrated using nuclear extracts from Hox-transfected COS cells and 12.5 days postcoitum mouse embryos or HOXB3 homeodomain-containing bacterial extracts. HOXB1, HOXB3, and nuclear extracts from 12.5 day mouse embryos bind to a sequence containing two palindromic TAATTA sites, which bear four copies of the ...
The number of down regulated genes ranged from 4. 7% in NTERA 2 R NTERA 2 to 37. 7% in NCCIT R NCCIT and 53. 3% in 2102EP R 2102EP cell line pairs. Thirty four overlapping genes were found in the two pairs NTERA 2 R NTERA 2 and NCCIT R NCCIT. The overlap ping number of differentially expressed genes was only 5 between cell line blog of sinaling pathways pairs NTERA 2 R NTERA 2 and 2102EP R 2102EP, and 6 between NCCIT R NCCIT and 2102EP R 2102EP. Only hsa miR 10b cell lines exposed to increasing concentrations of cisplatin resulted in a 2. 7 11. 3 increase in IC50 to the substance, as measured by the MTT assay 2,5 Diphenyltetrazolium Bromide. Whereas the IC50 values of the sensitive parental cell lines were 0. 45 uM, 0. 75 uM, and 1. 75 uM, respectively, the three cisplatin resistant sublines exhibited IC50 values of 5.. 1 uM in NTERA 2R, 4. 9 uM Inhibitors,Modulators,Libraries in 2102EP R, and 4. 7 uM in NCCIT R. Results of the cytotoxicity experiments have been reported else where. and has miR ...
Figure 3: The cells were biopsied directly from the primary tumors of the patients diagnosed with the embryonal carcinoma of the testes (huECT bio) (in the figure 3, the patients encoded 001-009), the mononuclear cells from bone marrow (huBMMC) and from peripheral blood (huPBMC), the cultured human embryonic stem cell lines (H1, H13, H14) (huESC cc), and the cultured cells from metastasis to lungs of the testicular embryonal carcinoma (NT2D1) (huECT cc), labeled with the superparamagnetic Fvs targeting TRA-1-6- and SSEA-4, and isolated with magnetic sorter to enrich the samples purity better than 99.5% with the statistical significance accepted at p , 0.001 ...
Many studies over the past 10 years, culminating in the recent report of the International Stem Cell Initiative (ISCI, 2011) have shown that hPSC acquire genetic and epigenetic changes during their time in culture. Many of the genetic changes are non-random and recurrent, probably because they provide a selective growth advantage to the undifferentiated cells. Some are shared by embryonal carcinoma cells, the malignant counterparts of ES cells. The origins of these growth advantages are poorly understood, but may come from altered cell cycle dynamics, resistance to apoptosis or altered patterns of differentiation. Less is known about the nature and consequences of epigenetic changes, but it is likely that these similarly affect hPSC behaviour; e.g., enhanced expression of DLK1, an imprinted gene, is associated with altered hPSC growth (Enver et al 2005). Inevitably, these genetic and epigenetic changes will impact on our ability to use hPSC for regenerative medicine, either because malignant ...
Spinal muscular atrophy (SMA) is a common neurodegenerative disease that is caused by mutations in the survival of motor neuron gene (SMN), leading to reduced levels of the SMN protein in affected individuals. In SMA, motor neurons selectively degenerate, however, the mechanism of cell death and the precise role of SMN in this process are not completely understood. In this study, we apply RNA interference (RNAi) to knockdown Smn gene expression in the murine embryonal carcinoma stem cell line P19, which can be differentiated into neuronal cells. A direct effect of Smn loss on apoptotic cell death in differentiated P19 neuronal cells, and to a lesser extent in undifferentiated cells was observed. Apoptosis could be partly reversed by expression of an SMN rescue construct, was reversible by the addition of the caspase-inhibitor ZVAD-fmk and involved the cytochrome c pathway. This study shows for the first time that knockdown of SMN results in apoptosis in mammalian neuronal cells and has implications for
ERV can be put to physiological use by their hosts, either at the gene regulatory level or as proteins. Tissue-specific enhancers in the ERV LTR are an example of transcriptional control. All mammals express amylase in the pancreas, but rodents and Old World primates also express amylase in salivary glands. In both the cases, ERV elements play a role in salivary expression in remarkably convergent evolution. The activation of salivary amylase in the human parotid gland is controlled by a retroviral insertion, which occurred during Old World primate evolution [54]. Then gene triplication of the amylase gene and its LTR enhancer to further enhance amylase secretion occurred after hominids split from chimpanzees. It may have provided selective advantage to the hominid lineage when, like rodents, they adopted a diet containing complex carbohydrates.. Many years ago, it was noted that MLV is not transcriptionally active in murine embryonal carcinoma stem cells, but that during differentiation into ...
Human tissue samples. Fresh frozen human nonneoplastic brain tissue and human tumor samples were obtained from the Department of Pathology at Brigham and Womens Hospital. All human materials were used in accordance with the policies of institutional review board at Brigham and Womens Hospital.. Cell lines and culturing conditions. Early-passage (passage 3) cultures from four independent human high-grade gliomas were a generous gift from Dr. David Louis (Massachusetts General Hospital). From each of the four high-grade gliomas, three cultures were established to give a total of 12 early-passage cultures (passage 3) used in our studies. Human glioblastoma cell lines A172, U87, LN229, U373, LN428, and LN308 were kindly donated by Drs. Azad Bonni and Rosalind Segal (Harvard University). All glioblastoma cell lines were maintained in DMEM supplemented with 10% fetal bovine serum (FBS). Undifferentiated P19 mouse embryonal carcinoma cells were cultured in αMEM supplemented with 10% FBS and were ...
TY - JOUR. T1 - Expression and functional significance of HtrA1 loss in endometrial cancer. AU - Mullany, Sally A.. AU - Moslemi-Kebria, Mehdi. AU - Rattan, Ramandeep. AU - Khurana, Ashwani. AU - Clayton, Amy. AU - Ota, Takayo. AU - Mariani, Andrea. AU - Podratz, Karl C.. AU - Chien, Jeremy. AU - Shridhar, Viji. PY - 2011/2/1. Y1 - 2011/2/1. N2 - Purpose: The purpose of this study was to determine if loss of serine protease HtrA1 in endometrial cancer will promote the invasive potential of EC cell lines. Experimental design: Western blot analysis and immunohistochemistry methods were used to determine HtrA1 expression in EC cell lines and primary tumors, respectively. Migration, invasion assays and in vivo xenograft experiment were performed to compare the extent of metastasis between HtrA1 expressing and HtrA1 knocked down clones. Results: Western blot analysis of HtrA1 in 13 EC cell lines revealed complete loss of HtrA1 expression in all seven papillary serous EC cell lines. Downregulation of ...
The development of tumor cell differentiation agents is new initiative in cancer treatment research. The goal of this project was to identify breast cancer differentiation agents by screening quinoline ring-containing compounds obtained form National Cancer Institute Compound Library. Of six differentiation-inducing quinolines NSC3852 was chosen as a lead compound. Our results demonstrate that NSC3852 is an inhibitor of HDAC activity in HeLa and MCF-7 cells nuclear extracts. NSC3852 caused superoxide generation in MCF-7 cells in a NADPH oxidase-dependent fashion, and NSC3852-induced oxidative stress led to the shift in a redox potential of the cells to a more oxidized state. This change in redox status of the cells was accompanied by the accumulation of hypophosphorylated pRb, downregulation of E2F-1 and Myc transcription factor protein levels, and cell differentiation. Superoxide formation in response to NSC3852 exposure caused DNA damage and subsequently apoptosis. MCF-7 cells growth was inhibited.
1. National Cancer Institute. NCI Thesaurus Version 18.11d. https://ncit.nci.nih.gov/ncitbrowser/ [2018-08-28]. [2018-09-21].. 2. The AACR Project GENIE Consortium. AACR Project GENIE: powering precision medicine through an international consortium. Cancer Discovery. 2017;7(8):818-831. Dataset Version 8. This dataset does not represent the totality of the genetic landscape; see paper for more information.. 3. All assertions and clinical trial landscape data are curated from primary sources. You can read more about the curation process here.. ...
Background: Ras homolog gene family member A (RhoA) is involved in Wnt-5a-induced migration of gastric and breast cancer cells. We investigated the roles of RhoA and Wnt-5a in ovarian carcinoma. Methods: RhoA and Wnt-5a mRNA and protein expression in normal fallopian tube epithelium, benign tumors, primary ovarian carcinomas, and metastatic omentum were quantified. RhoA or Wnt-5a was knocked down in OVCAR3 ovarian carcinoma cells using siRNAs and cell phenotype and expression of relevant molecules were assayed. Results: RhoA and Wnt-5a mRNA and protein expression were found to be significantly higher in metastatic omentum than in ovarian carcinomas, benign tumors, and normal fallopian tube epithelium (p < 0.05), and positively associated with differentiation and FIGO staging (stage I/II vs. stage III/IV) in ovarian carcinoma (p < 0.05). RhoA and Wnt-5a expression were positively correlated in ovarian carcinoma (p = 0.001, R2 = 0.1669). RhoA or Wnt-5a knockdown downregulated RhoA and Wnt-5a
In contrast to many other genes containing a CpG island, the testis-specific H2B (TH2B) histone gene exhibits tissue-specific methylation patterns in correlation with gene activity. Characterization of the methylation patterns within a 20-kb segment containing the TH2A and TH2B genes in comparison with that in a somatic histone cluster revealed that: (i) the germ cell-specific unmethylated domain of the TH2A and TH2B genes is defined as a small region surrounding the CpG islands of the TH2A and TH2B genes and (ii) somatic histone genes are unmethylated in both liver and germ cells, like other genes containing CpG islands, whereas flanking sequences are methylated. Transfection of in vitro-methylated TH2B, somatic H2B, and mouse metallothionein I constructs into F9 embryonal carcinoma cells revealed that the CpG islands of the TH2A and TH2B genes were demethylated like those of the somatic H2A and H2B genes and the metallothionein I gene. The demethylation of those CpG islands became ...
We previously discovered a pericellular matrix keratan sulphate/chondroitin sulphate proteoglycan present about the surface of human being embryonal carcinoma stem cells, cells whose differentiation mimics early development. sulphate glycosaminoglycans (analyzed in Funderburgh, 2000). Within an previous research, we reported the purification from the matrix-associated proteoglycan from individual EC Epothilone B cells (Cooper et al. 1992). A lot of the materials therefore isolated was within an aggregated type. While keratan chondroitin and sulphate sulphate accounted for every one of the glycosaminoglycan articles from the pericellular matrix type, just chemical deglycosylation attained comprehensive removal of glucose residues, to reveal primary protein rings of Mr 55 and 48 kDa. Weve observed previously which the proteoglycan could possibly be discovered in culture moderate by immunoassay (Pera et al. 1988). Therefore, a fresh purification protocol originated to review the secreted type of ...
gp200 renal cell carcinoma marker, 0.1 mg. |p|gp200 is a surface membrane glycoprotein expressed on human embryonal carcinoma and is a malignant stem cell of testicular tumors.
Clone REA157 recognizes the TRA-1-60 antigen, a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, which is a transmembrane glycoprotein. Podocalyxin is a member of the CD34-related family of sialomucins and is recognized as a stem cell and tumor marker. TRA-1-60 antigen is expressed on the surface of human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonic carcinoma cells (EC). Expression of TRA-1-60 is lost upon differentiation and thus it can be used to assess the differentiation stage of the cells. TRA-1-60 can be detected in the serum of patients with germ cell tumors which contain an EC component.Additional information: Clone REA157 displays negligible binding to Fc receptors. - Belgique
Downregulation of miR-320a/383-sponge-like long non-coding RNA NLC1-C (narcolepsy candidate-region 1 genes) is associated with male infertility and promotes testicular embryonal carcinoma cell proliferation. Lü M, et al. Cell Death and Disease, 2015 ...
Because embryonal carcinoma can differentiate into cells of all three germ layers, P19 cells can also differentiate into those ... When embryonal carcinoma cells are cultured at high density, they start to differentiate. By aggregating the cells into an ... van der Heyden, MA; Defize, LH (2003-05-01). "Twenty one years of P19 cells: what an embryonal carcinoma cell line taught us ... For developmental biologists, embryonal carcinoma, which is derived from teratocarcinoma, is a good object for developmental ...
embryonal carcinoma, ICD-O 9070/3). *mieszany rak gruczołowy (ang. mixed adenocarcinoma, ICD-O 8255/3) ... high grade neuroendocrine carcinoma, ICD-O 8246/3) *z dużych komórek (ang. large cell neuroendocrine carcinoma, ICD-O 8013/3) ... squamous cell carcinoma, ICD-O 8070/3). *rak z limfoidnym podścieliskiem (rdzeniasty) (ang. carcinoma with lymphoid stroma ( ... signet ring cell carcinoma and other variants; poorly cohesive carcinoma, ICD-O 8490/3) ...
Gerard, Smith, Austin (1986). "Genetic analysis of embryonal carcinoma cells". hdl:1842/20194. Cite journal requires ,journal ...
... embryonal carcinoma, teratocarcinoma, and choriocarcinoma; clinical and microscopical analysis (20 min; color). PMF 5316 (1957 ...
metaplastic carcinoma [18] Sarcoma botryoides no botryoid sarcoma, botryoid rhabdomyosarcoma; subtype of embryonal ... Micrograph of a small-cell carcinoma showing cells with nuclear moulding, minimal amount of cytoplasm and stippled chromatin. ... these include leukemia and most forms of carcinoma in situ. Tumor is also not synonymous with cancer. While cancer is by ...
Schopperle WM, Kershaw DB, DeWolf WC (Jan 2003). "Human embryonal carcinoma tumor antigen, Gp200/GCTM-2, is podocalyxin". ...
Boulter CA, Wagner EF (March 1988). "The effects of v-src expression on the differentiation of embryonal carcinoma cells". ... Przyborski SA (2001). "Isolation of human embryonal carcinoma stem cells by immunomagnetic sorting". Stem Cells. 19 (6): 500-4 ... "The small heat shock protein hsp25 is accumulated in P19 embryonal carcinoma cells and embryonic stem cells of line BLC6 during ... acid represses a cassette of candidate pluripotency chromosome 12p genes during induced loss of human embryonal carcinoma ...
Zhang L, Yoshida K, Liu J, Rosenberg RD (1999). "Anticoagulant heparan sulfate precursor structures in F9 embryonal carcinoma ...
Piccolo was a running back for the Bears from 1966 until his untimely death from embryonal cell carcinoma on June 16, 1970, at ... When Piccolo died in 1970, embryonal cell carcinoma was 100% fatal. With advances in medicine over the years, more than 50% of ... He was then diagnosed with embryonal cell carcinoma, an aggressive form of testicular cancer that had already spread to his ...
The most common specific subtypes are intratubular embryonal carcinoma and intratubular seminoma. GCNIS is seen in the ... unspecified variant of the entity was once considered to be a carcinoma in situ, although the term "carcinoma in situ" is now ... Carcinoma in situ Germ cell tumor ITGCN. H&E stain. Bettocchi C, Coker CB, Deacon J, Parkinson C, Pryor JP (1994). "A review of ... Dieckmann KP, Skakkebaek NE (December 1999). "Carcinoma in situ of the testis: review of biological and clinical features". Int ...
"Switched alternative splicing of oncogene CoAA during embryonal carcinoma stem cell differentiation". Nucleic Acids Research. ...
... cells and embryonal carcinoma (EC) cells: opposite sides of the same coin". 》Biochemical Society Transactions》 33 (Pt 6): 1526- ... "MULTIPOTENTIALITY OF SINGLE EMBRYONAL CARCINOMA CELLS". 》Cancer Research》 24: 1544-1551. ISSN 0008-5472. PMID 14234000.. ...
"Gap junction blockage interferes with neuronal and astroglial differentiation of mouse P19 embryonal carcinoma cells". Dev. ...
Jones-Villeneuve EM, McBurney MW, Rogers KA, Kalnins VI (1982). "Retinoic acid induces embryonal carcinoma cells to ... Historically, embryonic carcinoma (EC) cells have also been used. Fibroblasts or other differentiated cell types have been used ...
"The secreted glycoprotein CREG enhances differentiation of NTERA-2 human embryonal carcinoma cells". Oncogene. 19 (17): 2120-8 ...
Aizawa T, Maruyama K, Kondo H, Yoshikawa K (1992). "Expression of necdin, an embryonal carcinoma-derived nuclear protein, in ...
1984). "Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Differentiation in vivo ... NTERA-2 cells were originally isolated from a lung metastasis from a 22-year-old male patient with primary embryonal carcinoma ... The NTERA-2 (also designated NTERA2/D1, NTERA2, or NT2) cell line is a clonally derived, pluripotent human embryonal carcinoma ... Andrews PW (1984). "Retinoic acid induces neuronal differentiation of a cloned human embryonal carcinoma cell line in vitro". ...
Schoorlemmer J, Kruijer W (December 1991). "Octamer-dependent regulation of the kFGF gene in embryonal carcinoma and embryonic ... knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma ...
... embryonal carcinoma) cell line". Developmental Brain Research. 84 (1): 130-41. doi:10.1016/0165-3806(94)00166-W. PMID 7720212. ...
"AP-1 and Krox-24 transcription factors activate the neurofilament light gene promoter in P19 embryonal carcinoma cells". Cell ...
In pluripotent embryonal carcinoma cells, LIN28 is localized in the ribosomes, P-bodies and stress granules. LIN28 is thought ... "Micro-RNA regulation of the mammalian lin-28 gene during neuronal differentiation of embryonal carcinoma cells". Molecular and ...
"Renal cell carcinoma antigen is expressed by yolk sac tumors and yolk sac elements of embryonal carcinomas". Applied ... "A new gene coding for an antigen recognized by autologous cytolytic T lymphocytes on a human renal carcinoma". Immunogenetics. ... of RAGE-1 and MAGE-9 peptide-specific cytotoxic T-lymphocyte lines for transfer in patients with renal cell carcinoma". ...
It is the most common testicular tumor in children under 3, and is also known as infantile embryonal carcinoma. This age group ... particularly teratoma and embryonal carcinoma. While pure teratoma is usually benign, endodermal sinus tumor is malignant. ...
... embryonal carcinoma & yolk sac tumor (11%), seminoma & embryonal carcinoma (16%), and teratoma & embryonal carcinoma (26%). The ... embryonal carcinoma, gonadoblastoma, teratoma & yolk sac tumor Mixed GCTs: a combination of EGCTs. Common combinations include ...
"Downregulation of microRNA-383 is associated with male infertility and promotes testicular embryonal carcinoma cell ...
... of a 54K Dalton cellular SV40 tumor antigen present in SV40-transformed cells and uninfected embryonal carcinoma cells". Cell. ... naturally infects humans and is associated with Merkel cell carcinoma (MCC), a rare form of skin cancer originating from Merkel ... "Clonal Integration of a Polyomavirus in Human Merkel Cell Carcinoma". Science. 319 (5866): 1096-1100. Bibcode:2008Sci... ...
The potential role for human JADE1 in the renewal of embryonic stem cell and embryonal carcinoma cell cultures was suggested in ... "A data integration approach to mapping OCT4 gene regulatory networks operative in embryonic stem cells and embryonal carcinoma ... In a handful of pilot studies JADE1 expression was examined in colon cancers and renal carcinomas. The results in these studies ... The human pVHL is mutated in von Hippel-Lindau hereditary disease, and in majority of sporadic clear cell renal carcinomas. ...
KIT antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas ...
... embryonal carcinoma). The cancer had spread to his lymph nodes, lungs, brain, and abdomen. He visited urologist Jim Reeves in ...
Embryonal carcinomas, a rare tumor type usually found in mixed tumors, develop directly from germ cells but are not terminally ... Histologically, embryonal carcinoma appears similar to the embryonic disc, made up of epithelial, anaplastic cells in ... There are five main subtypes of ovarian carcinoma, of which high-grade serous carcinoma (HGSC) is the most common. These ... Serous carcinomas may develop from serous tubal intraepithelial carcinoma, rather than developing spontaneously from ovarian ...
F9 embryonal carcinoma cells are similar to the P19 cells shown in Figure 1 and normally have cell-to-cell adhesion mediated by ... Catenin and EMT interactions may also play a role in hepatocellular carcinoma. VEGF-B treatment of hepatoma carcinoma cells can ... Kobielak A, Fuchs E (February 2006). "Links between alpha-catenin, NF-kappaB, and squamous cell carcinoma in skin". Proc. Natl ... In another example, Wnt/β-catenin signaling has been identified as activating microRNA-181s in hepatocellular carcinoma that ...
It is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ ...
Embryonal carcinoma. *Endodermal sinus tumor/Yolk sac tumor. *Teratoma: Fetus in fetu ...
Zeegers MP, Jellema A, Ostrer H (2003). "Empiric risk of prostate carcinoma for relatives of patients with prostate carcinoma: ... Small cell carcinoma is a very rare (1%[77]) type of prostate cancer that cannot be diagnosed using the PSA.[77][78] As of 2009 ... "Immunohistochemical differentiation of high-grade prostate carcinoma from urothelial carcinoma". Am. J. Surg. Pathol. 31 (8): ... The upregulation of BCL-2 after androgen ablation in prostate carcinoma cell lines and in a castrated-male rat model further ...
Metastatic carcinoma. *Giant-cell tumor of the tendon sheath. *Glomus tumor. *Granular cell tumor ...
Embryonal carcinoma. *Endodermal sinus tumor. *Gonadoblastoma. *Teratoma/Struma ovarii. *Choriocarcinoma. Fibroma. *Meigs ...
"Relation of Height and Body Mass Index to Renal Cell Carcinoma in Two Million Norwegian Men and Women". American Journal of ... "Embryonal risks in gestational diabetes mellitus". Early Human Development. 79 (1): 59-63. doi:10.1016/j.earlhumdev.2004.04. ...
... or botryoid sarcoma[1] or botryoid rhabdomyosarcoma is a subtype of embryonal rhabdomyosarcoma, that can be ... Squamous-cell carcinoma of the vagina. *Botryoid rhabdomyosarcoma. *Clear cell adenocarcinoma of the vagina ... Hilgers R (1975). "Pelvic exenteration for vaginal embryonal rhabdomyosarcoma: a review". Obstet Gynecol. 45 (2): 175-80. PMID ... Reynolds E, Logani S, Moller K, Horowitz I (2006). "Embryonal rhabdomyosarcoma of the uterus in a postmenopausal woman. Case ...
"Gap junction blockage interferes with neuronal and astroglial differentiation of mouse P19 embryonal carcinoma cells". Dev. ...
... of all-trans retinoic acid dependent expression of HOXB and HOXC homeogenes in human embryonal and small-cell lung carcinoma ...
... embryonal carcinoma) cell line". Developmental Brain Research. 84 (1): 130-41. doi:10.1016/0165-3806(94)00166-W. PMID 7720212. ...
Carcinoma *Extramammary Paget's disease. *Bowen's disease. *Bowenoid papulosis. *Erythroplasia of Queyrat. *Hirsuties coronae ...
Embryonal carcinoma. *Endodermal sinus tumor. *Gonadoblastoma. *Teratoma/Struma ovarii. *Choriocarcinoma. Fibroma. *Meigs ...
Carcinoma *Extramammary Paget's disease. *Bowen's disease. *Bowenoid papulosis. *Erythroplasia of Queyrat. *Hirsuties coronae ...
Cai X, Yu K, Zhang L, Li Y, Li Q, Yang Z, Shen T, Duan L, Xiong W, Wang W. Synergistic inhibition of colon carcinoma cell ... GLI inhibitor GANT-61 diminishes embryonal and alveolar rhabdomyosarcoma growth by inhibiting Shh/AKT-mTOR axis. Oncotarget. ... Vismodegib is currently used for the treatment of metastatic basal cell carcinoma (BCC) in adults. It is also used for treating ... Identification of mutations in the human PATCHED gene in sporadic basal cell carcinomas and in patients with the basal cell ...
... signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene ... "Expression of the GLI2 oncogene and its isoforms in human basal cell carcinoma". The British Journal of Dermatology. 148 (5): ... resembling those of basal cell carcinoma (BCC). Unlike Gli1 transgenic mice, Gli2 transgenic mice only developed BCC-like ...
It plays an important role during embryonal development as programmed cell death and accompanies a variety of normal ... In particular, overexpression of HSP72 has been linked to the development some cancers, such as hepatocellular carcinoma, ... Therefore, the vaccine holds promise for treating hepatocellular carcinoma. Alternatively, overexpression of Hsp70 can mitigate ...
... embryonic stem cells or embryonal carcinoma cells). There is a positive correlation between serum bone alkaline phosphatase (B- ... One study compared the enzymes of liver metastases of giant-cell lung carcinoma and nonmalignant placental cells. The two were ...
... is highly expressed in the embryonal brain, and in numerous tissues in the embryo and adult organism. Elevated expression ... and renal cell carcinoma cells. A non-coding RNA, named B2, extending from 10 kb upstream of NEDD9 exon 1 to exon 4 has been ... in renal cell carcinoma". J. Cancer Res. Clin. Oncol. 140 (3): 387-97. doi:10.1007/s00432-013-1577-z. PMID 24384875. S2CID ... "Human enhancer of filamentation 1 Is a mediator of hypoxia-inducible factor-1alpha-mediated migration in colorectal carcinoma ...
... may refer to: The NTERA-2 human embryonal carcinoma cell line The Nam Theun 2 Dam, a hydroelectric dam located on the Nam ...
... hepatocellular carcinomas, bladder cancers, ovarian serous epithelial cancers, head and neck carcinomas, endometrial cancer, ... Embryonal precursors of Wilms tumor. Science 366(6470):1247-1251 Ayesh S, Matouk I, Schneider T, Ohana P, Laster M, Al-Sharef W ... In hepatocellular carcinoma, the expression of H19 and IGF2 usually changes from monoallelic to biallelic. In in vitro studies ... While the functions of the H19 RNA in the cell are still unclear, its presence in the many types of carcinoma cells suggest ...
... such as breast carcinoma and osteosarcoma. In these wild-type PAX3-expressing cancers, PAX3 function impacts on the control of ... embryonal rhabdomyosarcoma). However, PAX3 is also expressed in other cancer types without a clear relationship to a PAX3- ...
Squamous cell carcinoma not otherwise specified Basal cell carcinoma Squamous intraepithelial neoplasia Benign squamous lesions ... of other types Adenoma of minor vestibular glands Mixed Tumors of the vulva Tumors of skin appendage origin Embryonal ... these include leukemia and most forms of carcinoma in situ. Tumor is also not synonymous with cancer. While cancer is by ... Paget disease Bartholin gland Tumors: carcinomas, adenoma and adenomyoma Tumor arising from specialized ano-genital mammary- ...
... nuclear factor/retinoid receptor-related testis-associated receptor and its differential regulation during embryonal carcinoma ...
Definition of embryonal carcinoma. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and ... embryonal carcinoma. Definition: a malignant neoplasm of the testis or ovary, composed of anaplastic cells with indistinct ...
Embryonal carcinoma is a germ cell tumor characterized by primitive epithelial cells with marked pleomorphism and various ... Intratubular embryonal carcinoma is commonly associated with embryonal carcinoma (see the image below). Intratubular embryonal ... About 3-10% of pure germ cell tumors are embryonal carcinomas, and embryonal carcinomas are present in more than 80% of mixed ... Intratubular embryonal carcinoma. Dense, eosinophilic, necrotic debris in association with embryonal carcinoma cells is filling ...
Males with pure embryonal carcinoma tend to have a normal amount of the protein alpha-fetoprotein in the fluid component of ... Embryonal carcinoma is a relatively uncommon type of germ cell tumour that occurs in the ovaries and testes. The presenting ... The clinical features associated with metastasising embryonal carcinoma may include low back pain, dyspnoea, cough, haemoptysis ...
An embryonal carcinoma is a malignant growth that develops in germ cells. Common types of embryonal carcinomas include ... An embryonal carcinoma is a malignant growth which develops in germ cells. These types of cancers can be seen in men, women, ... Treatment for an embryonal carcinoma can be supervised by an oncologist, who will work with other members of a medical team ... In men, embryonal carcinomas such as seminomas are often responsible for testicular cancer. If the cancer is allowed to grow, ...
Fifteen of the 54 germ cell tumors containing embryonal carcinoma were also examined with antibodies to CD30. These embryonal ... The embryonal carcinoma of the testis is a germ cell neoplasm. Clinically, these tumors present much like other testicular ... OCT4 Staining in Testicular Tumors: A Sensitive and Specific Marker for Seminoma and Embryonal Carcinoma.. Jones TD, Ulbright ... It has been detected in primary testicular germ cell tumors with pluripotent potential, seminoma, and embryonal carcinoma. We ...
Disease relevance of Carcinoma, Embryonal. *Mouse teratocarcinoma stem cells (embryonal carcinoma, or EC cells) bind very small ... Biological context of Carcinoma, Embryonal. *Murine embryonal carcinoma cells (ECCs) do not express antigens of the major ... High impact information on Carcinoma, Embryonal. *All of these properties are induced in undifferentiated embryonal carcinoma ... Analytical, diagnostic and therapeutic context of Carcinoma, Embryonal. *Indirect immunofluorescence on embryonal carcinoma ...
The mouse embryonal carcinoma lines PCC4-F and F9 have played important roles in the isolation and characterization of the two ... Previous Document: P19 embryonal carcinoma cells.. Next Document: Midkine (MK), the product of a retinoic acid responsive gene ... The mouse embryonal carcinoma lines PCC4-F and F9 have played important roles in the isolation and characterization of the two ...
Using NTera2/clone D1 (NT2/D1) human embryonal carcinoma cells as a model, we report that the RA induced terminal ... Overexpression of the retinoic acid receptor gamma directly induces terminal differentiation of human embryonal carcinoma cells ...
... and infantile embryonal carcinoma. They are a type of germ cell tumor that can be found in infants and adults. ... Yolk sac tumors (YST) are also known as endodermal sinus tumors (EST) and infantile embryonal carcinoma. ...
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
In the course of retinoic acid-induced differentiation of embryonal carcinoma cells the epitope disappeared before the onset of ... Unusual stage-specific embryonic antigen (TEC-4) defined by a monoclonal antibody to embryonal carcinoma cells defective in the ... One monoclonal antibody prepared by this strategy was found to react specifically with mouse embryonal carcinoma and embryo- ... Unusual stage-specific embryonic antigen (TEC-4) defined by a monoclonal antibody to embryonal carcinoma cells defective in the ...
... Oncogene. ... Specific siRNA to p53 prevents cisplatin-mediated activation of p53 and p53 pathway genes and renders embryonal carcinoma cells ... we define immediate transcriptional targets in response to cisplatin in testicular germ cell-derived human embryonal carcinoma ...
... *Authors: *Guimiao Lin ... The purpose of this study was to investigate the effect of TCF3 on embryonal carcinoma (EC). The mouse F9 EC cell line and a ... Lin, G., Zhao, L., Yin, F., Lan, R., Li, L., Zhang, X., Zhang, H., Yang, B.TCF3 inhibits F9 embryonal carcinoma growth by the ... Lin, G., Zhao, L., Yin, F., Lan, R., Li, L., Zhang, X., Zhang, H., Yang, B.TCF3 inhibits F9 embryonal carcinoma growth by the ...
... acid acid-triggered differentiation of pluripotent cells towards neural progenitors derived from human embryonal carcinoma stem ... Tegenge, M.A.; Roloff, F.M.; Bicker, G. Rapid differentiation of human embryonal carcinoma stem cells (NT2) into neurons for ... Genomic and Phenotypic Alterations of the Neuronal-Like Cells Derived from Human Embryonal Carcinoma Stem Cells (NT2) Caused by ... neurodifferentiation; neurodevelopmental toxicity; NT2; organophosphorus pesticides; NTE; human embryonal carcinoma stem cells ...
... Choroid plexus carcinoma. *High grade glioma (including anaplastic astrocytoma, anaplastic oligodendroglioma, anaplastic ... Neoplasms, Germ Cell and Embryonal. Neoplasms, Nerve Tissue. Neoplasms by Site. Nervous System Diseases. Cyclophosphamide. ...
The Human Embryonal Carcinoma Marker Antigen TRA-1-60 Is a Sialylated Keratan Sulfate Proteoglycan. Graeme Badcock, Christine ... Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors ( ... The Human Embryonal Carcinoma Marker Antigen TRA-1-60 Is a Sialylated Keratan Sulfate Proteoglycan ... The Human Embryonal Carcinoma Marker Antigen TRA-1-60 Is a Sialylated Keratan Sulfate Proteoglycan ...
Proviral sequences that restrict retroviral expression in mouse embryonal carcinoma cells.. T P Loh, L L Sievert, R W Scott ... Embryonal carcinoma (EC) cells are nonpermissive for retrovirus replication. Restriction of retroviral expression in EC cells ... Proviral sequences that restrict retroviral expression in mouse embryonal carcinoma cells. Message Subject (Your Name) has ...
Characterization of constitutive HSF2 DNA-binding activity in mouse embryonal carcinoma cells.. S P Murphy, J J Gorzowski, K D ... Mouse embryonal carcinoma (EC) cells are the only mammalian cells known to exhibit HSE-binding activity, as determined by gel ... Characterization of constitutive HSF2 DNA-binding activity in mouse embryonal carcinoma cells. ... Characterization of constitutive HSF2 DNA-binding activity in mouse embryonal carcinoma cells. ...
Mesodermal differentiation of embryonal carcinoma cells in coculture with visceral endoderm cell lines PIERSMA A. H. ... Retinoic acid resistance of the variant embryonal carcinoma cell line rac65 is caused by expression of a truncated rar alpha ... ggregation and cell cycle dependent retinoic acid receptor mRNA expression in P19 embryonal carcinoma cells JONK L. J. C. ... Isolation of male embryonal carcinoma cells and their chromosome replication patterns MCBURNEY M. W. ...
... and human embryonal carcinoma cells (ECs) if we are to exploit these cells in regenerative medicine regimes. Correlating gene ...
Defective expression of adenovirus genes during early infection of undifferentiated OTF963 embryonal carcinoma cells.. C C ... These results suggest limits to the generality and explanatory power of the hypothesis that F9 embryonal carcinoma cells ... Defective expression of adenovirus genes during early infection of undifferentiated OTF963 embryonal carcinoma cells. ... Defective expression of adenovirus genes during early infection of undifferentiated OTF963 embryonal carcinoma cells. ...
... and quantitative determination of percentage of the primary tumor composed of embryonal carcinoma, yolk sac carcinoma, teratoma ... Percentage of Embryonal Carcinoma and of Vascular Invasion Predicts Pathological Stage in Clinical Stage I Nonseminomatous ... Percentage of Embryonal Carcinoma and of Vascular Invasion Predicts Pathological Stage in Clinical Stage I Nonseminomatous ... Percentage of Embryonal Carcinoma and of Vascular Invasion Predicts Pathological Stage in Clinical Stage I Nonseminomatous ...
DNMT3L Is a Novel Marker and Is Essential for the Growth of Human Embryonal Carcinoma. Kahori Minami, Tokuhiro Chano, Takahiro ... embryonal carcinoma, EC), embryonal-like somatic differentiated (teratoma), and extraembryonally differentiated ( ... Ki-1 (CD30) antigen is regularly expressed by tumor cells of embryonal carcinoma. Am J Pathol 1988;133:446-50. ... We then studied the role of DNMT3L in TGCTs by the treatment of two embryonal carcinoma (EC) cell lines with a small ...
Friends leukemia cell, which can be differentiated by activin, and embryonal carcinoma(EC) cells(PCC3, P19 and F9), were found ...
Embryonal Carcinoma of Pediatric CNS, Embryonal Carcinoma of the Pediatric CNS, Pediatric CNS Embryonal Cell Carcinoma, ... Embryonal Carcinoma of the Pediatric Central Nervous System, Embryonal Carcinoma of the Childhood CNS, Embryonal Carcinoma of ... Embryonal Carcinoma of Childhood CNS, Childhood CNS Embryonal Cell Carcinoma, Embryonal Carcinoma of the Childhood Central ... Pediatric Central Nervous System Embryonal Carcinoma, Embryonal Carcinoma of Pediatric Central Nervous System, ...
Differentiation of human embryonal carcinoma cells induces human immunodeficiency virus permissiveness which is stimulated by ... Over-expression of the retinoic acid receptor gamma directly induces the terminal differentiation of human embryonal carcinoma ... Differentiation of tera 2 human embryonal carcinoma cells into neurons and human cytomegalovirus permissive cells induction by ... Heat shock induces differentiation of human embryonal carcinoma cells into trophectoderm lineages. Experimental Cell Research. ...
Human embryonal carcinoma (EC) cells comprise the pluripotent stem cells of malignant non-seminomatous germ cell tumors (GCTs) ... Ectopic activation of WNT signaling in human embryonal carcinoma cells and its effects in short- and long-term in vitro culture ... Ectopic activation of WNT signaling in human embryonal carcinoma cells and its effects in short- and long-term in vitro culture ...
Substantial multiplication in vitro of cloned cells from a human embryonal carcinoma cell line, Tera 2, has been obtained in a ... Proliferation of a human embryonal carcinoma-derived cell line in serum-free medium: inter-relationship between growth factor ... Proliferation of a human embryonal carcinoma-derived cell line in serum-free medium: inter-relationship between growth factor ... Proliferation of a human embryonal carcinoma-derived cell line in serum-free medium: inter-relationship between growth factor ...
Retinoic acid induces embryonal carcinoma cells to differentiate into neurons and glial cells. J Cell Biol. 1982;94:253-262. ... Smooth muscle actin expression during P19 embryonal carcinoma differentiation in cell culture. J Cell Physiol. 1990;142:89-98. ... Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line. Nature. 1982;299:165-167. ... In this study, we describe a model system in which multipotential mouse P19 embryonal carcinoma cells (P19s) can be induced to ...
Fig. 8. The parameters of proliferation in control and differentiating A.1 and 1.3 EC cells. EC cells of both lines seeded in the same densities (2.5 x 103 cells/cm2) were cultured with and without 10-6 M RA for 24, 48, 72, and 96 h, respectively. A, growth rates. Cells were lysed in SDS-containing buffer, and the total amounts of protein were used as a measure of cell quantities. Micrograms of total protein are shown on the Y axis. Data are presented as the means of three independent experiments; bars, SE. B, the distribution of cells in cell cycle phases. Cells were fixed in Vindelov s solution, stained with propidium iodide, and analyzed using FACSCalibur equipped with ModFit 2.0 software. The percentages of cells in G1, S, and G2 phases are expressed as the means of three independent experiments; bars, SE.. ...
  • Embryonal carcinoma is one of the most common germ cell tumors. (medscape.com)
  • About 3-10% of pure germ cell tumors are embryonal carcinomas, and embryonal carcinomas are present in more than 80% of mixed germ cell tumors. (medscape.com)
  • As with other germ cell tumors, with the exception of spermatocytic seminoma , embryonal carcinomas are believed to originate from intratubular malignant germ cells. (medscape.com)
  • [ 5 ] In the vast majority of cases, IGCNU occurs adjacent to invasive germ cell tumors, including embryonal carcinomas. (medscape.com)
  • Embryonal carcinomas are aggressive germ cell tumors. (medscape.com)
  • Although serum alpha fetoprotein (AFP) has been reported in association with embryonal carcinomas, most of those cases were probably misclassified as such and in truth represented mixed cases of embryonal carcinomas and yolk sac tumors . (medscape.com)
  • OCT4 Staining in Testicular Tumors: A Sensitive and Specific Marker for Seminoma and Embryonal Carcinoma. (thedoctorsdoctor.com)
  • It has been detected in primary testicular germ cell tumors with pluripotent potential, seminoma, and embryonal carcinoma. (thedoctorsdoctor.com)
  • We examined histologic sections from 91 primary testicular neoplasms, including 64 cases of mixed germ cell tumors containing embryonal carcinoma (54), seminoma (51), yolk sac tumor (38), mature teratoma (31), immature teratoma (20), and choriocarcinoma (15). (thedoctorsdoctor.com)
  • Fifteen of the 54 germ cell tumors containing embryonal carcinoma were also examined with antibodies to CD30. (thedoctorsdoctor.com)
  • Yolk sac tumors (YST) are also known as endodermal sinus tumors (EST) and infantile embryonal carcinoma . (knowcancer.com)
  • Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). (aacrjournals.org)
  • Human embryonal carcinoma (EC) cells comprise the pluripotent stem cells of malignant non-seminomatous germ cell tumors (GCTs) and represent the malignant counterpart of embryonic stem cells (ESCs). (eur.nl)
  • Any of a diverse group of malignancies, such as carcinoid, islet cell tumors, neuroblastoma, and small-cell carcinomas of the lung. (thefreedictionary.com)
  • Embryonal carcinomas are classified as nonseminoma germ cell tumors . (cdc.gov)
  • Determine the feasible dose and duration of carboplatin combined with craniospinal and local radiotherapy and adjuvant chemotherapy in children with newly diagnosed, high-risk CNS embryonal tumors (Phase I completed as of 11-25-03). (knowcancer.com)
  • TGCTs are classified into two major histologic subgroups, seminoma and nonseminoma: nonseminomatous TGCTs show embryonal and extraembryonal differentiation patterns, which include primitive zygotic (embryonal carcinoma, EC), embryonal-like somatic differentiated (teratoma), and extraembryonally differentiated (choriocarcinoma, yolk sac tumor) phenotypes ( 2 ). (aacrjournals.org)
  • chorionic carcinoma choriocarcinoma . (thefreedictionary.com)
  • In all cases of mixed germ cell tumor with components of embryonal carcinoma (54) and seminoma (51), there was greater than 90% nuclear staining of the embryonal carcinoma and seminoma tumor cells with little to no background staining. (thedoctorsdoctor.com)
  • Primary tumor pathological material was analyzed for vascular invasion, lymphatic invasion, tunical invasion, and quantitative determination of percentage of the primary tumor composed of embryonal carcinoma, yolk sac carcinoma, teratoma, and seminoma. (aacrjournals.org)
  • Univariate logistic regression analyses revealed that vascular invasion ( P = 0.0001), percentage of embryonal carcinoma ( P = 0.0001), lymphatic invasion ( P = 0.0001), and tunical invasion ( P = 0.0013) were higher in pathological stage II and that percentage of teratoma ( P = 0.0001) and of yolk sac carcinoma ( P = 0.0174) were higher in stage I. Percentage of seminoma was not significant. (aacrjournals.org)
  • The gene expression patterns of human ES cell lines showed many similarities with the human embryonal carcinoma cell samples and more distantly with the seminoma samples. (pnas.org)
  • Polyclonal antibodies were used to assay human embryonal carcinoma (EC), differentiating EC, yolk sac carcinoma, and teratoma cells for expression of viral early antigen (T-Ag) after infection with simian virus 40 (SV40). (uthscsa.edu)
  • After infection with SV40, T-Ag was expressed by 1%, or less, of the cells (presumed to be differentiated derivatives) in only some EC cultures whereas the antigen was synthesized by a significant percentage of the yolk sac carcinoma, teratoma, and differentiating EC cells. (uthscsa.edu)
  • Linzer, DIH , Maltzman, W & Levine, AJ 1979, ' Characterization of a murine cellular SV40 T antigen in SV40-transformed cells and uninfected embryonal carcinoma cells ', Symposia on Quantitative Biology , vol. 44, no. 1, pp. 215-224. (northwestern.edu)
  • Persistance of CD30 expression in embryonal carcinoma (EC) cells: a retrospective study in multi-relapsed or chemotherapy (CT) refractory germ-cell. (esmo.org)
  • 1995 ) A dynamic balance between ARP-1/COUP-TFII, EAR-3/COUP-TFI, and retinoic acid receptor:retinoid X receptor heterodimers regulates Oct-3/4 expression in embryonal carcinoma cells. (biologists.org)
  • PATIENTS METHODS Biopsies were obtained from the patients with the clinical and histological diagnoses of the primary, pure embryonal carcinomas of the testes. (semanticscholar.org)
  • RESULTS The clones of cells, with the strong surface display of TRA-1-60 and SSEA-4, were identified and isolated directly from the biopsies acquired from the patients diagnosed with the pure embryonal carcinomas of the testes. (semanticscholar.org)
  • CONCLUSION In the pure embryonal carcinomas of the testes, acquired directly from the patients, we identified, isolated with high viability and selectivity, and profiled the clones of the pluripotent stem cells. (semanticscholar.org)
  • TRA-1-60(+), SSEA-4(+), POU5F1(+), SOX2(+), NANOG(+) Clones of Pluripotent Stem Cells in the Embryonal Carcinomas of the Testes. (semanticscholar.org)
  • Embryonal carcinoma is a germ cell tumor characterized by primitive epithelial cells with marked pleomorphism and various histologic patterns. (medscape.com)
  • adenocystic carcinoma ( adenoid cystic carcinoma ) carcinoma marked by cylinders or bands of hyaline or mucinous stroma separated or surrounded by nests or cords of small epithelial cells, occurring in the mammary and salivary glands, and mucous glands of the respiratory tract. (thefreedictionary.com)
  • basal cell carcinoma the most common form of skin cancer, consisting of an epithelial tumor of the skin originating from neoplastic differentiation of basal cells , rarely metastatic but locally invasive and aggressive. (thefreedictionary.com)
  • bronchioalveolar carcinoma ( bronchiolar carcinoma ) ( bronchioloalveolar carcinoma ) ( bronchoalveolar carcinoma ) a variant type of adenocarcinoma of the lung , with columnar to cuboidal epithelial cells lining the alveolar septa and projecting into alveolar spaces in branching papillary formations. (thefreedictionary.com)
  • giant cell carcinoma a poorly differentiated, highly malignant, epithelial neoplasm containing many large multinucleated tumor cells, such as occurs in the lungs. (thefreedictionary.com)
  • medullary carcinoma that composed mainly of epithelial elements with little or no stroma. (thefreedictionary.com)
  • In the course of retinoic acid-induced differentiation of embryonal carcinoma cells the epitope disappeared before the onset of morphological differentiation. (pnas.org)
  • Lamins A and C appear during retinoic acid-induced differentiation of mouse embryonal carcinoma cells. (rupress.org)
  • The lamin complement of nuclear matrix isolated from F9 embryonal carcinoma cells was studied during retinoic acid-induced differentiation in culture. (rupress.org)
  • In the present study, we treated primary glioblastoma, rhabdomyosarcoma, hepatocellular carcinoma and human embryonic carcinoma cells (NCCIT) with μ-molar concentrations of genistein and assessed mitotic index, cell morphology, global gene expression, and specific cell-cycle regulating genes. (biomedcentral.com)
  • cholangiocellular carcinoma a rare type of hepatocellular carcinoma arising from the cholangioles , consisting of two layers of cells surrounding a minute lumen. (thefreedictionary.com)
  • hepatocellular carcinoma primary carcinoma of the liver cells with hepatomegaly, jaundice, hemoperitoneum, and other symptoms of the presence of an abdominal mass. (thefreedictionary.com)
  • Association of embryonal carcinomas with syncytiotrophoblastic giant cells may cause increases in serum human chorionic gonadotropin (hCG) levels. (medscape.com)
  • Overexpression of the retinoic acid receptor gamma directly induces terminal differentiation of human embryonal carcinoma cells. (nih.gov)
  • Using NTera2/clone D1 (NT2/D1) human embryonal carcinoma cells as a model, we report that the RA induced terminal differentiation of these cells into a neuronal phenotype is characterized by an increase in expression of RAR alpha, RAR beta, RAR gamma, and a slight induction of RXR alpha. (nih.gov)
  • Through use of gene-expression array profiling we define immediate transcriptional targets in response to cisplatin in testicular germ cell-derived human embryonal carcinoma cells. (nih.gov)
  • In this study, we have shown that DNMT3L, a fetal-specific DNA methyltransferase-like protein, is a novel marker and is essential for the growth of human embryonal carcinoma (EC). (aacrjournals.org)
  • Substantial multiplication in vitro of cloned cells from a human embryonal carcinoma cell line, Tera 2, has been obtained in a basal medium (DMEM/Ham's F12,50:50, v/v) supplemented with 10 micrograms low density lipoprotein/ml, 100 micrograms high density lipoprotein/ml, 100 ng multiplication stimulating activity/ml, 100 ng insulin/ml and 1 microgram transferrin/ml. (biologists.org)
  • Expression Profiling of Lineage Differentiation in Pluripotential Human Embryonal Carcinoma Cells -- Houldsworth et al. (aacrjournals.org)
  • An in vitro model of apoptosis and differentiation in human embryonal carcinoma (EC) cells was developed to study the mode of cell death and mechanisms of cell death in early development. (elsevier.com)
  • We identified 895 genes that were expressed at significantly greater levels in human ES and embryonal carcinoma cell lines than in control samples. (pnas.org)
  • The objective of this study was to assess serum-free growth conditions for the human embryonal carcinoma cell line, NT2/D1. (atla.org.uk)
  • Cripto-1 (CR-1), a glycosylphosphatidylinositol-linked glycoprotein, is expressed during early embryogenesis and in various human carcinomas. (fujita-hu.ac.jp)
  • We demonstrated that human embryonal carcinoma (EC) cells are heterogeneous for CR-1 expression and consist of two distinct subpopulations: a CR-1 High and a CR-1 Low population. (fujita-hu.ac.jp)
  • Salomon, David S. / Cripto-1 is a cell surface marker for a tumorigenic, undifferentiated subpopulation in human embryonal carcinoma cells . (fujita-hu.ac.jp)
  • Embryonal carcinoma is a relatively uncommon type of germ cell tumour that occurs in the ovaries and testes. (wikipedia.org)
  • One monoclonal antibody prepared by this strategy was found to react specifically with mouse embryonal carcinoma and embryo-derived stem cell lines. (pnas.org)
  • Vascular invasion and determination of percentage of embryonal carcinoma should be assessed for all clinical stage I nonseminomatous germ cell tumor patients and the model presented herein can be used clinically to predict the likelihood of occult disease and dictate therapy. (aacrjournals.org)
  • We then studied the role of DNMT3L in TGCTs by the treatment of two embryonal carcinoma (EC) cell lines with a small interfering RNA system. (aacrjournals.org)
  • Friends leukemia cell, which can be differentiated by activin, and embryonal carcinoma(EC) cells(PCC3, P19 and F9), were found to express 3500-20,000 per cell of activin receptors. (nih.gov)
  • Differentiated clonal cell lines were isolated from pluripotent P19 embryonal carcinoma (EC) cells treated as aggregates with retinoic acid. (uu.nl)
  • 1. Basal cell carcinoma. (thefreedictionary.com)
  • Any slowly growing squamous cell carcinoma of the skin, typically presenting as a gradually enlarging warty tumor. (thefreedictionary.com)
  • Squamous cell carcinoma. (thefreedictionary.com)
  • It includes basal cell carcinomas, keratoacanthomas, and squamous cell carcinomas of the skin. (thefreedictionary.com)
  • adenocarcinoma of the lung , large cell carcinoma , small cell carcinoma , and squamous cell carcinoma . (thefreedictionary.com)
  • 2. renal cell carcinoma . (thefreedictionary.com)
  • cylindrical cell carcinoma carcinoma in which the cells are cylindrical or nearly so. (thefreedictionary.com)
  • Hürthle cell carcinoma a malignant Hürthle cell tumor . (thefreedictionary.com)
  • large cell carcinoma a type of bronchogenic carcinoma of undifferentiated (anaplastic) cells of large size, a variety of squamous cell carcinoma that has undergone further dedifferentiation. (thefreedictionary.com)
  • Testicular embryonal cell carcinoma presenting as haemoptysis and skin nodules. (bvsalud.org)
  • Embryonal cell carcinoma affects young males in the prime of their life with majority of tumours already having metastasised at the time of diagnosis . (bvsalud.org)
  • Fine needle aspiration cytology of skin nodules and the lung lesion led to the diognosis of testicular embryonal cell carcinoma . (bvsalud.org)
  • For such analysis H19 and Igf2 genes were activated by inducing differentiation of (mouse embryonal carcinoma cell x mouse lymphocyte) hybrid cell clones. (princeton.edu)
  • Tilghman, Shirley M. / Monoallelic expression of reactivated imprinted genes in embryonal carcinoma cell hybrids . (princeton.edu)
  • A case of primary embryonal-cell carcinoma of the parietal lobe is reported. (thejns.org)
  • Review of the literature yielded no previous reports of a lobar embryonal-cell carcinoma. (thejns.org)
  • Isolation of embryonal carcinoma cell lines that express integrated r" by M Taketo, E Gilboa et al. (jax.org)
  • Isolation of embryonal carcinoma cell lines that express integrated recombinant genes flanked by the Moloney murine leukemia virus long terminal repeat. (jax.org)
  • By infecting three embryonal carcinoma cell lines, PCC4.aza1R, F9tk-, and Nulli-SCC1, with this recombinant virus, we have isolated many transductant clones that stably express the integrated neomycin-resistance gene. (jax.org)
  • These clonal transductant lines consist of undifferentiated embryonal carcinoma cells as judged by morphology, tumorigenicity in 129/Sv mice, and cell-surface antigenic markers. (jax.org)
  • These results suggest that the expression of the neomycin-resistance gene, linked to MoMuLV LTR in the transductant embryonal carcinoma cell clones, is due to a cisacting mechanism(s). (jax.org)
  • Nonseminomatous GCTs, on the other hand, typically include teratocarcinomas with EC components, as well as cell types corresponding to the extra embryonic tissues, the yolk sac as yolk sac carcinomas (YSCs), and the trophoblast as choriocarcinomas. (pnas.org)
  • In this paper we have examined the growth and differentiation of the embryonal carcinoma cell line, F 9 , in the defined medium EM-3 at low density. (nebraska.edu)
  • Herein, using P19 embryonal carcinoma cells as a model, we observed that Plzf expression was induced in the P19-derived embryonic bodies, which enrich neural stem-like cell populations, as demonstrated by the expression of neural stem cell markers, Nestin and Sox2. (elsevier.com)
  • P19 cells is an embryonic carcinoma cell line derived from an embryo-derived teratocarcinoma in mice. (wikipedia.org)
  • Also, it is the most characterized embryonic carcinoma (EC) cell line that can be induced into cardiac muscle cells and neuronal cells by different specific treatments. (wikipedia.org)
  • These embryonal carcinoma components were all positive for CD30 with staining of greater than 90% of the tumor cells but with variable staining intensity. (thedoctorsdoctor.com)
  • embryonal tumor or embryonic tumor n. (definithing.com)
  • Most developmentally regulated epitopes identified on embryonal carcinoma cells and murine preimplantation embryos are associated with a glycoprotein-bound large glycan called embryoglycan. (pnas.org)
  • Murine F9 embryonal carcinoma (F9 EC) stem cells have an E1a-like transcription activity that is downregulated as these cells differentiate to parietal endoderm. (ox.ac.uk)
  • An embryonal carcinoma is a malignant growth which develops in germ cells . (wisegeek.com)
  • Both teratocarcinomas and seminomas appear to arise, initially, as abnormal germ cells that form carcinoma-in situ (CIS) within the seminiferous tubules ( 25 ). (pnas.org)
  • In men, embryonal carcinomas such as seminomas are often responsible for testicular cancer. (wisegeek.com)
  • Embryonal carcinoma is a type of testicular cancer , which is cancer that starts in the testicles, the male reproductive glands located in the scrotum. (cdc.gov)
  • We investigated the functional role of eosinophil cationic protein (ECP) in regulating cardiomyogenesis using mouse P19CL6 embryonic carcinoma cells. (elsevier.com)
  • P19 embryonal carcinoma cells were induced to differentiate into neuron-like cells using all trans-retinoic acid and the presence and/or function of cellular molecules, such as NgR1, NMDA receptors and STAT3, were examined. (kribb.re.kr)
  • Because embryonal carcinoma can differentiate into cells of all three germ layers, P19 cells can also differentiate into those ectoderm, mesoderm and endoderm-like cells. (wikipedia.org)
  • When embryonal carcinoma cells are cultured at high density, they start to differentiate. (wikipedia.org)
  • If the cancer is an embryonal carcinoma, treatment options can include surgery, chemotherapy, and radiation. (wisegeek.com)
  • A form of cancer , carcinoma makes up the majority of the cases of malignancy of the breast, uterus, intestinal tract, skin, and tongue. (thefreedictionary.com)
  • The transcription factor Elf3, which is one of over 25 Ets family members, is expressed in a wide variety of carcinomas and has been shown to promote the transcription of many genes implicated in cancer. (wustl.edu)
  • Subcutaneous metastasis from embryonal carcinoma is rare and is associated with widespread disease and poor prognosis . (bvsalud.org)
  • Other forms of embyonal carcinomas can include teratomas, choriocarcinomas, and yolk sac or endodermal sinus carcinomas, with these last being more common in young children. (wisegeek.com)
  • bronchogenic carcinoma any of a large group of carcinomas of the lung, so called because they arise from the epithelium of the bronchial tree. (thefreedictionary.com)
  • Teratocarcinomas are a particular type of GCT that contain undifferentiated stem cells, termed embryonal carcinoma (EC) cells, and differentiated derivatives that can include all three germ layers. (pnas.org)
  • An embryonal carcinoma that arises from the central nervous system and occurs during childhood. (mycancergenome.org)
  • A rare carcinoma that arises from pancreatic cells that manufacture digestive proteins, such as lipase, chymotrypsin, or alpha-1-antitrypsin. (thefreedictionary.com)
  • Disclaimer: Embryonal carcinoma definition / meaning should not be considered complete, up to date, and is not intended to be used in place of a visit, consultation, or advice of a legal, medical, or any other professional. (definithing.com)
  • Specific siRNA to p53 prevents cisplatin-mediated activation of p53 and p53 pathway genes and renders embryonal carcinoma cells relatively resistant to cisplatin cytotoxicity. (nih.gov)
  • Defective expression of adenovirus genes during early infection of undifferentiated OTF963 embryonal carcinoma cells. (asm.org)
  • MYCN, PD-L1, and TMB-High are the most frequent gene inclusion criteria for childhood central nervous system embryonal carcinoma clinical trials [ 3 ]. (mycancergenome.org)
  • Fusion of embryonal carcinoma cells to fibroblast cells, cytoplasts, and karyoplasts. (jax.org)
  • The mouse embryonal carcinoma lines PCC4-F and F9 have played important roles in the isolation and characterization of the two ubiquitous basement membrane proteins, laminin and entactin. (biomedsearch.com)
  • Proviral sequences that restrict retroviral expression in mouse embryonal carcinoma cells. (asm.org)
  • Characterization of constitutive HSF2 DNA-binding activity in mouse embryonal carcinoma cells. (asm.org)
  • Mouse embryonal carcinoma (EC) cells are the only mammalian cells known to exhibit HSE-binding activity, as determined by gel shift assays, even when maintained at normal physiological temperatures. (asm.org)
  • In this study, we describe a model system in which multipotential mouse P19 embryonal carcinoma cells (P19s) can be induced to express multiple characteristics of differentiated smooth muscle. (ahajournals.org)
  • Early Cycling-independent Changes to p27, Cyclin D2, and Cyclin D3 in Differentiating Mouse Embryonal Carcinoma Cells -- Preclíková et al. (aacrjournals.org)
  • Polyoma virus can develop lytically in differentiated mouse cells, but is unable to express either early or late functions in undifferentiated early embryonic cells or in embryonal carcinoma (EC) cells of the mouse. (pasteur.fr)