Carboxypeptidase H
Carboxypeptidases
Chromaffin Granules
Carboxypeptidase B
Carboxypeptidases A
Carboxypeptidases that are primarily found the DIGESTIVE SYSTEM that catalyze the release of C-terminal amino acids. Carboxypeptidases A have little or no activity for hydrolysis of C-terminal ASPARTIC ACID; GLUTAMIC ACID; ARGININE; LYSINE; or PROLINE. This enzyme requires ZINC as a cofactor and was formerly listed as EC 3.4.2.1 and EC 3.4.12.2.
Lysine Carboxypeptidase
Glu300 of rat carboxypeptidase E is essential for enzymatic activity but not substrate binding or routing to the regulated secretory pathway. (1/106)
Several recently discovered members of the carboxypeptidase E (CPE) gene family lack critical active site residues that are conserved in other family members. For example, three CPE-like proteins contain a Tyr in place of Glu300 (equivalent to Glu270 of carboxypeptidase A and B). To investigate the importance of this position, Glu300 of rat CPE was converted into Gln, Lys, or Tyr, and the proteins expressed in Sf9 cells using the baculovirus system. All three mutants were secreted from the cells, but the media showed no enzyme activity above background levels. Wild-type CPE and the Gln300 point mutant bound to a p-aminobenzoyl-Arg-Sepharose affinity resin, and this binding was competed by an active site-directed inhibitor, guanidinoethylmercaptosuccinic acid. The affinity purified mutant CPE protein showed no detectable enzyme activity (<0.004% of wild-type CPE) toward dansyl-Phe-Ala-Arg. Expression of the Gln300 and Lys300 mutant CPE proteins in the NIT3 mouse pancreatic beta-cell line showed that these mutants are routed into secretory vesicles and secreted via the regulated pathway. Taken together, these results indicate that Glu300 of CPE is essential for enzyme activity, but not required for substrate binding or for routing into the regulated secretory pathway. (+info)Biosynthesis and packaging of carboxypeptidase D into nascent secretory vesicles in pituitary cell lines. (2/106)
Metallocarboxypeptidase D (CPD) is a membrane-bound trans-Golgi network (TGN) protein. In AtT-20 cells, CPD is initially produced as a 170-kDa endoglycosidase H-sensitive glycoprotein. Within 30 min of chase, the CPD increases to 180 kDa and is resistant to endoglycosidase H as a result of carbohydrate maturation. CPD also undergoes an activation step required for binding to a substrate affinity resin. Blocking the protein exit from the endoplasmic reticulum inhibits the increase in molecular mass but not the step required for affinity column binding, suggesting that enzyme activation precedes carbohydrate maturation and that these reactions occur in distinct intracellular compartments. Only the higher molecular weight mature CPD enters nascent secretory vesicles, which bud from the TGN of permeabilized AtT-20 and GH3 cells. The budding efficiency of CPD into vesicles is 2-3-fold lower than that of endogenous proopiomelanocortin in AtT-20 cells or prolactin in GH3 cells. In contrast, the packaging of a truncated form of CPD, which lacks the cytoplasmic tail and transmembrane domain, was similar to that of proopiomelanocortin. Taken together, the results support the proposal that CPD functions in the TGN in the processing of proteins that transit the secretory pathway and that the C-terminal region plays a major role in TGN retention. (+info)Identification and characterization of proSAAS, a granin-like neuroendocrine peptide precursor that inhibits prohormone processing. (3/106)
Five novel peptides were identified in the brains of mice lacking active carboxypeptidase E, a neuropeptide-processing enzyme. These peptides are produced from a single precursor, termed proSAAS, which is present in human, mouse, and rat. ProSAAS mRNA is expressed primarily in brain and other neuroendocrine tissues (pituitary, adrenal, pancreas); within brain, the mRNA is broadly distributed among neurons. When expressed in AtT-20 cells, proSAAS is secreted via the regulated pathway and is also processed at paired-basic cleavage sites into smaller peptides. Overexpression of proSAAS in the AtT-20 cells substantially reduces the rate of processing of the endogenous prohormone proopiomelanocortin. Purified proSAAS inhibits prohormone convertase 1 activity with an IC(50) of 590 nM but does not inhibit prohormone convertase 2. Taken together, proSAAS may represent an endogenous inhibitor of prohormone convertase 1. (+info)Lipid raft association of carboxypeptidase E is necessary for its function as a regulated secretory pathway sorting receptor. (4/106)
Membrane carboxypeptidase E (CPE) is a sorting receptor for targeting prohormones, such as pro-opiomelanocortin, to the regulated secretory pathway in endocrine cells. Its membrane association is necessary for it to bind a prohormone sorting signal at the trans-Golgi network (TGN) to facilitate targeting. In this study, we examined the lipid interaction of CPE in bovine pituitary secretory granule membranes, which are derived from the TGN. We show that CPE is associated with detergent-resistant lipid domains, or rafts, within secretory granule membranes. Lipid analysis revealed that these rafts are enriched in glycosphingolipids and cholesterol. Pulse-chase and subcellular fractionation experiments in AtT-20 cells show that the association of CPE with membrane rafts occurred only after it reached the Golgi. Cholesterol depletion resulted in dissociation of CPE from secretory granule membranes and decreased the binding of prohormones to membranes. In vivo cholesterol depletion using lovastatin resulted in the lack of sorting of CPE and its cargo to the regulated secretory pathway. We propose that the sorting receptor function of CPE necessitates its interaction with glycosphingolipid-cholesterol rafts at the TGN, thereby anchoring it in position to bind to its prohormone cargo. (+info)Oligomerization of pro-opiomelanocortin is independent of pH, calcium and the sorting signal for the regulated secretory pathway. (5/106)
Studies indicate that pro-opiomelanocortin (POMC) is sorted to the regulated secretory pathway by binding to a sorting receptor identified as membrane-bound carboxypeptidase E (CPE) [Cool et al. (1997) Cell 88, 73-83]. The efficiency of this sorting mechanism could be enhanced if POMC molecules were to self-associate to form oligomers, prior or subsequent to binding to CPE. Using cross-linking and gel filtration techniques, we demonstrated that POMC forms oligomers at both neutral and acidic pHs and calcium was not necessary. delta N-POMC, which lacks the N-terminal sorting signal for the regulated secretory pathway, also formed similar oligomers, indicating that the sorting and oligomerization domains are different. (+info)Impaired prohormone convertases in Cpe(fat)/Cpe(fat) mice. (6/106)
A spontaneous point mutation in the coding region of the carboxypeptidase E (CPE) gene results in a loss of CPE activity that correlates with the development of late onset obesity (Nagert, J. K., Fricker, L. D., Varlamov, O., Nishina, P. M., Rouille, Y., Steiner, D. F., Carroll, R. J., Paigen, B. J., and Leiter, E. H. (1995) Nat. Genet. 10, 135-142). Examination of the level of neuropeptides in these mice showed a decrease in mature bioactive peptides as a result of a decrease in both carboxypeptidase and prohormone convertase activities. A defect in CPE is not expected to affect endoproteolytic processing. In this report we have addressed the mechanism of this unexpected finding by directly examining the expression of the major precursor processing endoproteases, prohormone convertases PC1 and PC2 in Cpe(fat) mice. We found that the levels of PC1 and PC2 are differentially altered in a number of brain regions and in the pituitary. Since these enzymes have been implicated in the generation of neuroendocrine peptides (dynorphin A-17, beta-endorphin, and alpha- melanocyte-stimulating hormone) involved in the control of feeding behavior and body weight, we compared the levels of these peptides in Cpe(fat) and wild type animals. We found a marked increase in the level of dynorphin A-17, a decrease in the level of alpha-melanocyte-stimulating hormone, and an alteration in the level of C-terminally processed beta-endorphin. These results suggest that the impairment in the level of these and other peptides involved in body weight regulation is mainly due to an alteration in carboxypeptidase and prohormone convertase activities and that this may lead to the development of obesity in these animals. (+info)ProSAAS processing in mouse brain and pituitary. (7/106)
ProSAAS is a newly discovered protein with a neuroendocrine distribution generally similar to that of prohormone convertase 1 (PC1), a peptide-processing endopeptidase. Several proSAAS-derived peptides were previously identified in the brain and pituitary of the Cpe(fat)/Cpe(fat) mouse based on the accumulation of C-terminally extended peptides due to the absence of enzymatically active carboxypeptidase E, a peptide-processing exopeptidase. In the present study, antisera against different regions of proSAAS were used to develop radioimmunoassays and examine the processing profile of proSAAS in wild type and Cpe(fat)/Cpe(fat) mouse tissues following gel filtration and reverse phase high performance liquid chromatography. In wild type mouse brain and pituitary, the majority of proSAAS is processed into smaller peptides. These proSAAS-derived peptides elute from the reverse-phase column in the same positions as synthetic peptides that correspond to little SAAS, PEN, and big LEN. Mass spectrometry revealed the presence of peptides with the expected molecular masses of little SAAS and big LEN in the fractions containing immunoreactive peptides. The processing of proSAAS is slightly impaired in Cpe(fat)/Cpe(fat) mice, relative to wild-type mice, leading to the accumulation of partially processed peptides. One of these peptides, the C-terminally extended form of PEN, is known to inhibit PC1 activity and this could account for the reduction in enzymatically active PC1 seen in Cpe(fat)/Cpe(fat) mice. The observation that little SAAS and big LEN are the major forms of these peptides produced in mouse brain and pituitary raises the possibility that these peptides function as neurotransmitters or hormones. (+info)Cholesterol, a cell size-dependent signal that regulates glucose metabolism and gene expression in adipocytes. (8/106)
Enlarged fat cells exhibit modified metabolic capacities, which could be involved in the metabolic complications of obesity at the whole body level. We show here that sterol regulatory element-binding protein 2 (SREBP-2) and its target genes are induced in the adipose tissue of several models of rodent obesity, suggesting cholesterol imbalance in enlarged adipocytes. Within a particular fat pad, larger adipocytes have reduced membrane cholesterol concentrations compared with smaller fat cells, demonstrating that altered cholesterol distribution is characteristic of adipocyte hypertrophy per se. We show that treatment with methyl-beta-cyclodextrin, which mimics the membrane cholesterol reduction of hypertrophied adipocytes, induces insulin resistance. We also produced cholesterol depletion by mevastatin treatment, which activates SREBP-2 and its target genes. The analysis of 40 adipocyte genes showed that the response to cholesterol depletion implicated genes involved in cholesterol traffic (caveolin 2, scavenger receptor BI, and ATP binding cassette 1 genes) but also adipocyte-derived secretion products (tumor necrosis factor alpha, angiotensinogen, and interleukin-6) and proteins involved in energy metabolism (fatty acid synthase, GLUT 4, and UCP3). These data demonstrate that altering cholesterol balance profoundly modifies adipocyte metabolism in a way resembling that seen in hypertrophied fat cells from obese rodents or humans. This is the first evidence that intracellular cholesterol might serve as a link between fat cell size and adipocyte metabolic activity. (+info)
Cloning and sequence analysis of cDNA for bovine carboxypeptidase E<...
Carboxypeptidase E - Wikipedia
Quantitation of neuropeptides in Cpe<sup>fat</sup>/Cpe<sup>fat</sup> mice using differential isotopic tags and...
Carboxypeptidase E (CPE) is a prohormone/proneuropeptide handling enzyme and mice bearing - How NF-B is activated: the role of...
2-Guanidinoethylmercaptosuccinic Acid - CAS 77482-44-1 - Calbiochem CAS 77482-44-1 | 369334
DI-fusion Cloning of candidate autoantigen carboxypeptidase H from a...
Drosophila carboxypeptidase D (SILVER) is a key enzyme in neuropeptide processing required to maintain locomotor activity...
CPE carboxypeptidase E [Homo sapiens (human)] - Gene - NCBI
CPE Enzyme Human Recombinant | Carboxypeptidase E | ProSpec
Acceptable CPE activities for certificate renewal | TCTA
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Sequence of human carboxypeptidase D reveals it to be a member of the regulatory carboxypeptidase family with three tandem...
Plus it
Intracellular trafficking of metallocarboxypeptidase D in AtT-20 cells: Localization to the trans-Golgi network and recycling...
Old, Fat Mice Blamed for Virus Tr... ( Contact with humans can lead to deadl...)
Identification and Characterization of proSAAS, a Granin-Like Neuroendocrine Peptide Precursor that Inhibits Prohormone...
Publications - Universität Ulm
Characterization of the Mouse Aortic Carboxypeptidase-Like Protein Promoter Reveals Activity in Differentiated and...
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Plus it
Carboxypeptidase
... an alanine carboxypeptidase bradykinin is broken down among other enzymes by carboxypeptidase N D-Ala carboxypeptidase is a ... Initial studies on carboxypeptidases focused on pancreatic carboxypeptidases A1, A2, and B in the digestion of food. Most ... Carboxypeptidases act by replacing the substrate water with a carbonyl (C=O) group. The carboxypeptidase A hydrolysis reaction ... Carboxypeptidase E Carboxypeptidase A Enzyme category EC number 3.4 Thrombin-activatable fibrinolysis inhibitor aka plasma ...
Carboxypeptidase P
The term carboxypeptidase P may refer to: Lysosomal Pro-X carboxypeptidase Membrane Pro-X carboxypeptidase This set index page ...
Muramoyltetrapeptide carboxypeptidase
... carboxypeptidase II, lysyl-D-alanine carboxypeptidase, L-lysyl-D-alanine carboxypeptidase, LD-carboxypeptidase) is an enzyme. ... Muramoyltetrapeptide+carboxypeptidase at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology ( ... Metz R, Henning S, Hammes WP (March 1986). "LD-carboxypeptidase activity in Escherichia coli. II. Isolation, purification and ... DasGupta H, Fan DP (July 1979). "Purification and characterization of a carboxypeptidase-transpeptidase of Bacillus megaterium ...
Glutamate carboxypeptidase
... (EC 3.4.17.11, carboxypeptidase G, carboxypeptidase G1, carboxypeptidase G2, glutamyl ... Glutamate carboxypeptidase II Goldman P, Levy CC (October 1967). "Carboxypeptidase G: purification and properties". Proceedings ... Glutamate+carboxypeptidase at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (EC 3.4.17). ... Sherwood RF, Melton RG, Alwan SM, Hughes P (May 1985). "Purification and properties of carboxypeptidase G2 from Pseudomonas sp ...
Carboxypeptidase B2
... (CPB2), also known as carboxypeptidase U (CPU), plasma carboxypeptidase B (pCPB) or thrombin-activatable ... Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, ... and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A ( ... "Entrez Gene: CPB2 carboxypeptidase B2 (plasma)". Bouma BN, Mosnier LO (2005). "Thrombin activatable fibrinolysis inhibitor ( ...
Carboxypeptidase E
... (CPE), also known as carboxypeptidase H (CPH) and enkephalin convertase, is an enzyme that in humans is ... "Entrez Gene: CPE carboxypeptidase E". Fricker LD (1988). "Carboxypeptidase E". Annual Review of Physiology. 50: 309-21. doi: ... Biology portal Carboxypeptidase Carboxypeptidase A GRCh38: Ensembl release 89: ENSG00000109472 - Ensembl, May 2017 GRCm38: ... fills in for carboxypeptidase E in this organism. In humans, CPE is encoded by the CPE gene. Carboxypeptidase E functions in ...
Dipeptidyl carboxypeptidase
... may refer to: Angiotensin-converting enzyme (ACE) Peptidyl-dipeptidase Dcp This set index page ...
Carboxypeptidase G
... may refer to: Glutamate carboxypeptidase, an enzyme Gamma-glutamyl hydrolase, an enzyme This set index page ...
Muramoylpentapeptide carboxypeptidase
D-alanine carboxypeptidase I, DD-carboxypeptidase, D-alanine carboxypeptidase, D-alanyl-D-alanine carboxypeptidase, D-alanine-D ... carboxypeptidase, carboxypeptidase D-alanyl-D-alanine, carboxypeptidase I, UDP-N-acetylmuramoyl-tetrapeptidyl-D-alanine alanine ... Muramoylpentapeptide+carboxypeptidase at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology ( ... Purification and properties of two D-alanine carboxypeptidases from Escherichia coli". The Journal of Biological Chemistry. 243 ...
Carboxypeptidase U
... (EC 3.4.17.20, arginine carboxypeptidase, carboxypeptidase R, plasma carboxypeptidase B, thrombin- ... Wang W, Hendriks DF, Scharpé SS (June 1994). "Carboxypeptidase U, a plasma carboxypeptidase with high affinity for plasminogen ... plasma is activated by thrombin or plasmin during clotting to form the unstable carboxypeptidase U. Carboxypeptidase Eaton DL, ... Carboxypeptidase+U at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (EC 3.4.17). ...
Carboxypeptidase C
... (EC 3.4.16.5, carboxypeptidase Y, serine carboxypeptidase I, cathepsin A, lysosomal protective protein, ... Carboxypeptidase+C at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (EC 3.4.16). ... Cathepsin A Breddam, K. (1986). "Serine carboxypeptidases. A review". Carlsberg Res. Commun. 51: 83-128. doi:10.1007/bf02907561 ... deamidase, lysosomal carboxypeptidase A, phaseolin) is an enzyme. This enzyme catalyses the following chemical reaction Release ...
Carboxypeptidase A
... inhibitor Carboxypeptidase B Carboxypeptidase Carboxypeptidase E Christianson DW, Lipscomb WN (February 1989 ... This property of carboxypeptidase A led to the first clause of Daniel E. Koshland, Jr.'s "induced fit" hypothesis. The S1 sub- ... Carboxypeptidase A (CPA) contains a zinc (Zn2+) metal center in a tetrahedral geometry with amino acid residues in close ... Carboxypeptidase A usually refers to the pancreatic exopeptidase that hydrolyzes peptide bonds of C-terminal residues with ...
Carboxypeptidase A6
Carboxypeptidase A inhibitor Carboxypeptidase GRCh38: Ensembl release 89: ENSG00000165078 - Ensembl, May 2017 GRCm38: Ensembl ... Carboxypeptidase A6 (CPA6) is a metallocarboxypeptidase enzyme that in humans is encoded by the CPA6 gene. It is highly ... "Entrez Gene: Carboxypeptidase A6". Retrieved 2011-11-25. Lyons PJ, Callaway MB, Fricker LD (March 2008). "Characterization of ... The protein encoded by this gene belongs to the family of carboxypeptidases, which catalyze the release of C-terminal amino ...
Arginine carboxypeptidase
... may refer to: Lysine carboxypeptidase, an enzyme Carboxypeptidase U, an enzyme This set index page ...
Carboxypeptidase M
... (EC 3.4.17.12, CPM) is an enzyme. This enzyme catalyses the following chemical reaction Cleavage of C- ... Carboxypeptidase+M at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (EC 3.4.17). ... Deddish PA, Skidgel RA, Erdös EG (July 1989). "Enhanced Co2+ activation and inhibitor binding of carboxypeptidase M at low pH. ... Similarity to carboxypeptidase H (enkephalin convertase)". The Biochemical Journal. 261 (1): 289-91. PMC 1138816. PMID 2775217 ...
Carboxypeptidase A2
"Structure of a novel leech carboxypeptidase inhibitor determined free in solution and in complex with human carboxypeptidase A2 ... Carboxypeptidase A2 is an enzyme that in humans is encoded by the CPA2 gene. Three different forms of human pancreatic ... "Entrez Gene: CPA2 carboxypeptidase A2 (pancreatic)". Pascual R, Burgos FJ, Salva M, et al. (1989). "Purification and properties ... Human Carboxypeptidase A2) at the PDBe-KB. Portal: Biology v t e (Genes on human chromosome 7, EC 3.4.17, All stub articles, ...
Carboxypeptidase T
... intracellular carboxypeptidase of Thermoactinomycetes--a distant analog of animal carboxypeptidase]". Biokhimiia. 49 (2): 292- ... Carboxypeptidase T (EC 3.4.17.18, CPT) is an enzyme. This enzyme catalyses the following chemical reaction: Releases a C- ... Carboxypeptidase+T at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (EC 3.4.17). ... Osterman AL, Stepanov VM, Rudenskaia GN, Khodova OM, Tsaplina IA (February 1984). "[Carboxypeptidase T-- ...
Carboxypeptidase A1
... is an enzyme that in humans is encoded by the CPA1 gene. Three different forms of human pancreatic ... "Entrez Gene: CPA1 carboxypeptidase A1 (pancreatic)". Catasús L, Villegas V, Pascual R, et al. (1992). "cDNA cloning and ... Carboxypeptidase A1 is a monomeric pancreatic exopeptidase. It is involved in zymogen inhibition. GRCh38: Ensembl release 89: ... Stewart EA, Craik CS, Hake L, Bowcock AM (1990). "Human carboxypeptidase A identifies a BglII RFLP and maps to 7q31-qter". Am. ...
Alanine carboxypeptidase
Alanine+carboxypeptidase at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (EC 3.4.17). ... Alanine carboxypeptidase (EC 3.4.17.6, N-benzoyl-L-alanine-amidohydrolase) is an enzyme. This enzyme catalyses the following ...
Zinc carboxypeptidase
The carboxypeptidase A family can be divided into two subfamilies: carboxypeptidase H (regulatory) and carboxypeptidase A ( ... "Primary structure of carboxypeptidase T: delineation of functionally relevant features in Zn-carboxypeptidase family". J. ... Structural studies of carboxypeptidases A and B reveal the propeptide to exist as a globular domain, followed by an extended ... Members of the carboxypeptidase A family are synthesised as inactive molecules with propeptides that must be cleaved to ...
Carboxypeptidase Taq
"Purification and characterization of a thermostable carboxypeptidase (carboxypeptidase Taq) from Thermus aquaticus YT-1". ... Carboxypeptidase Taq (EC 3.4.17.19) is an enzyme. This enzyme catalyses the following chemical reaction Release of a C-terminal ... Carboxypeptidase+Taq at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (EC 3.4.17). ... Lee SH, Taguchi H, Yoshimura E, Minagawa E, Kaminogawa S, Ohta T, Matsuzawa H (August 1994). "Carboxypeptidase Taq, a ...
Gly-X carboxypeptidase
Gly-Xaa carboxypeptidase (EC 3.4.17.4, glycine carboxypeptidase, carboxypeptidase a, carboxypeptidase S, peptidase alpha, yeast ... Gly-Xaa+carboxypeptidase at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (EC 3.4.17). ... carboxypeptidase) is an enzyme. This enzyme catalyses the following chemical reaction Release of a C-terminal amino acid from a ...
Glutamate carboxypeptidase II
... (GCPII), also known as N-acetyl-L-aspartyl-L-glutamate peptidase I (NAALADase I), NAAG peptidase ... All of which refer to the same protein glutamate carboxypeptidase II. GCPII is mainly expressed in four tissues of the body, ... and carboxypeptidase activity based on the parent tissue. The hydrolysis of NAAG by GCPII obeys Michaelis-Menten kinetics. ...
Carboxypeptidase A inhibitor
The structure of the complex between bovine carboxypeptidase A and the 39-amino-acid carboxypeptidase A inhibitor from potatoes ... Hass GM, Nau H, Biemann K, Grahn DT, Ericsson LH, Neurath H (March 1975). "The amino acid sequence of a carboxypeptidase ... In molecular biology, the carboxypeptidase A inhibitor family is a family of proteins which is represented by the well- ... Rees DC, Lipscomb WN (August 1980). "Structure of the potato inhibitor complex of carboxypeptidase A at 2.5-A resolution". Proc ...
Potato carboxypeptidase inhibitor
PCI also inhibits carboxypeptidase R without affecting the activity of carboxypeptidase N in the circulation and have therefore ... May 1998). "Potato carboxypeptidase inhibitor, a T-knot protein, is an epidermal growth factor antagonist that inhibits tumor ... Potato carboxypeptidase inhibitor (PCI) is a naturally occurring protease inhibitor peptide in potatoes that can form complexes ... a Redlitz A, Tan AK, Eaton DL, Plow EF (November 1995). "Plasma carboxypeptidases as regulators of the plasminogen system". J. ...
Tubulinyl-Tyr carboxypeptidase
... carboxypeptidase-tubulin, soluble carboxypeptidase, tubulin-tyrosine carboxypeptidase, tubulin carboxypeptidase, ... tyrosinotubulin carboxypeptidase, tyrosyltubulin carboxypeptidase, TTCPase, brain I carboxypeptidase) is an enzyme. This enzyme ... Tubulinyl-Tyr+carboxypeptidase at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (EC 3.4. ... Kumar N, Flavin M (July 1981). "Preferential action of a brain detyrosinolating carboxypeptidase on polymerized tubulin". The ...
Membrane Pro-X carboxypeptidase
Membrane Pro-Xaa carboxypeptidase (EC 3.4.17.16, carboxypeptidase P, microsomal carboxypeptidase) is an enzyme. This enzyme ... Membrane+Pro-Xaa+carboxypeptidase at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (EC ... Isolation of a microsomal carboxypeptidase from swine kidney". European Journal of Biochemistry. 17 (2): 372-7. doi:10.1111/j. ... Purification and properties of carboxypeptidase P from pig kidneys". The Biochemical Journal. 229 (1): 251-7. doi:10.1042/ ...
Lysosomal Pro-X carboxypeptidase
... aminoacylproline carboxypeptidase, prolyl carboxypeptidase, carboxypeptidase P, proline-specific carboxypeptidase P, PCP) is an ... Lysosomal Pro-Xaa carboxypeptidase (EC 3.4.16.2, angiotensinase C, lysosomal carboxypeptidase C, peptidylprolylamino acid ... Lysosomal+Pro-Xaa+carboxypeptidase at the US National Library of Medicine Medical Subject Headings (MeSH) human ...
Zinc D-Ala-D-Ala carboxypeptidase
D-alanyl-D-alanine-cleaving carboxypeptidase, DD-carboxypeptidase, G enzyme, DD-carboxypeptidase-transpeptidase) is an enzyme. ... Zinc+D-Ala-D-Ala+carboxypeptidase at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (EC ... Zinc D-Ala-D-Ala carboxypeptidase (EC 3.4.17.14, Zn2+ G peptidase, D-alanyl-D-alanine hydrolase, ... "The complete amino acid sequence of the Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase of streptomyces albus G". ...
Carboxypeptidase x, m14 family member 2
... is a protein that in humans is encoded by the CPXM2 gene. GRCh38: Ensembl release 89: ... "Entrez Gene: Carboxypeptidase X, M14 family member 2". Retrieved 2018-06-01. Grupe A, Li Y, Rowland C, Nowotny P, Hinrichs AL, ...
Cpvl MGI Mouse Gene Detail - MGI:1918537 - carboxypeptidase, vitellogenic-like
CPM (carboxypeptidase M)
Carboxypeptidase M in apoptosis, adipogenesis and cancer.. Denis CJ et al. 23294303. 2013. The potential of carboxypeptidase M ... Carboxypeptidase M in Madin-Darby canine kidney cells. Evidence that carboxypeptidase M has a phosphatidylinositol glycan ... Molecular cloning and sequencing of the cDNA for human membrane-bound carboxypeptidase M. Comparison with carboxypeptidases A, ... CPM (carboxypeptidase M). 2013-07-01 Anne-Marie Lambeir Affiliation Laboratory of Medical Biochemistry, University of Antwerp, ...
RCSB PDB - 1AC5: CRYSTAL STRUCTURE OF KEX1(DELTA)P, A PROHORMONE-PROCESSING CARBOXYPEPTIDASE FROM SACCHAROMYCES CEREVISIAE
In contrast to yeast serine carboxypeptidase (CPD-Y) and wheat serine carboxypeptidase II (CPDW-II), Kex1p displays a very ... In contrast to yeast serine carboxypeptidase (CPD-Y) and wheat serine carboxypeptidase II (CPDW-II), Kex1p displays a very ... CRYSTAL STRUCTURE OF KEX1(DELTA)P, A PROHORMONE-PROCESSING CARBOXYPEPTIDASE FROM SACCHAROMYCES CEREVISIAE. *PDB DOI: 10.2210/ ... Crystal structure of Kex1deltap, a prohormone-processing carboxypeptidase from Saccharomyces cerevisiae.. Shilton, B.H., Thomas ...
ENZYME - 3.4.17.24 tubulin-glutamate carboxypeptidase
Porcine Carboxypeptidase B Purified Enzyme - Innovative Research
Chymotrypsin and trypsin 0.02%. Carboxypeptidase B is an enzyme involved in the digestion of food. A protease enzyme, ... Porcine Carboxypeptidase B Purified Enzyme from Innovative Research has been chromatographically purified. This is a frozen ... A protease enzyme, Carboxypeptidase B hydrolyzes peptide bonds at the C-terminal end of a protein or peptide. Carboxypeptidases ... Product Inquiry for Porcine Carboxypeptidase B Purified Enzyme. Name. Email. Phone Number. Message. ...
Missense polymorphism in the human carboxypeptidase E gene alters enzymatic activity<...
Carboxypeptidase E (CPE) is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. One of ... N2 - Carboxypeptidase E (CPE) is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. One ... AB - Carboxypeptidase E (CPE) is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. One ... Missense polymorphism in the human carboxypeptidase E gene alters enzymatic activity. Piero Nicolao, Massimo Carella, Bruno ...
Orally active glutamate carboxypeptidase II inhibitor 2-MPPA attenuates dizocilpine-induced prepulse inhibition deficits in...
Takatsu, Y., Fujita, Y., Tsukamoto, T., Slusher, B. S., & Hashimoto, K. (2011). Orally active glutamate carboxypeptidase II ... Takatsu Y, Fujita Y, Tsukamoto T, Slusher BS, Hashimoto K. Orally active glutamate carboxypeptidase II inhibitor 2-MPPA ... N2 - Glutamate carboxypeptidase II (GCP II) is a glial enzyme responsible for the hydrolysis of N-acetylaspartylglutamate (NAAG ... AB - Glutamate carboxypeptidase II (GCP II) is a glial enzyme responsible for the hydrolysis of N-acetylaspartylglutamate (NAAG ...
Carboxypeptidase M/CPM蛋白, Mouse (422a.a, HEK293, His) | MCE
MCE提供Carboxypeptidase M/CPM蛋白, Mouse (422a.a, HEK293, His),体外功能蛋白活性验证,出色的批间稳定性, ... Carboxypeptidase M/CPM Protein, Mouse (422a.a, HEK293, His) 相关产品:. * Carboxypeptidase M/CPM Protein, Mouse (406a.a, HEK293, His ... Carboxypeptidase M/CPM 蛋白, Mouse (422a.a, HEK293, His) 目录号: HY-P73494 Data Sheet 产品使用指南 ... Carboxypeptidase M/CPM Protein, Mouse
Muramoylpentapeptide Carboxypeptidase | Profiles RNS
"Muramoylpentapeptide Carboxypeptidase" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, ... This graph shows the total number of publications written about "Muramoylpentapeptide Carboxypeptidase" by people in this ... Below are the most recent publications written about "Muramoylpentapeptide Carboxypeptidase" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Muramoylpentapeptide Carboxypeptidase". ...
Toxins | Free Full-Text | Morphologic, Phylogenetic and Chemical Characterization of a Brackish Colonial Picocyanobacterium ...
The fractionated extracts showed significant activity against carboxypeptidase A and trypsin. Inhibition of these important ... The carboxypeptidase A inhibition assays were modified from described methods [62,63]. Carboxypeptidase A from bovine pancreas ... Hass, G.M.; Ager, S.P.; Le Tourneau, D.; Derr-Makus, J.E. Specificity of the carboxypeptidase inhibitor from potatoes. Plant ... APs are the only known cyanobacteria peptide inhibitors of the pancreatic metalloexopeptidase carboxypeptidase A [5,37,39,40]. ...
Analysis of the carboxypeptidase D cytoplasmic domain: Implications in intracellular trafficking<...
Analysis of the carboxypeptidase D cytoplasmic domain: Implications in intracellular trafficking. Elena Kalinina, Oleg Varlamov ... Analysis of the carboxypeptidase D cytoplasmic domain: Implications in intracellular trafficking. Journal of cellular ... Analysis of the carboxypeptidase D cytoplasmic domain : Implications in intracellular trafficking. / Kalinina, Elena; Varlamov ... Kalinina, E., Varlamov, O., & Fricker, L. D. (2002). Analysis of the carboxypeptidase D cytoplasmic domain: Implications in ...
Glutamate Carboxypeptidase II</span><span class=...
Glutamate carboxypeptidase II (GCPII) is a 94 kD class II membrane bound zinc metalloenzyme which catalyzes the hydrolysis of ... Barinka C, Rojas C, Slusher BS, Pomper M. "Glutamate carboxypeptidase II in diagnosis and treatment of neurologic disorders and ... Johns Hopkins Drug Discovery - Project - Glutamate Carboxypeptidase II. 53 page-template,page-template-full_width,page-template ... "Discovery of Orally Available Prodrugs of the Glutamate Carboxypeptidase II (GCPII) Inhibitor 2-Phosphonomethylpentanedioic ...
Zn-dependent arginine carboxypeptidase-like family domain assignments
Zn-dependent arginine carboxypeptidase-like family domain assignments . Domain assignment details for each protein include ... Zn-dependent arginine carboxypeptidase-like family domain assignments No domain assignments for these genomes.. Add assignments ... View all assignments containing a Zn-dependent arginine carboxypeptidase-like domain in each group of genomes. ...
Zinc in PDB 4gm5: Carboxypeptidase T with Sulphamoil Arginine
Enzymatic activity of Carboxypeptidase T with Sulphamoil Arginine. All present enzymatic activity of Carboxypeptidase T with ... S.A.Kuznetsov, V.I.Timofeev, V.K.Akparov, I.P.Kuranova. Carboxypeptidase T with Sulphamoil Arginine To Be Published. ... The structure of Carboxypeptidase T with Sulphamoil Arginine, PDB code: 4gm5 was solved by S.A.Kuznetsov, V.I.Timofeev, V.K. ... The structure of Carboxypeptidase T with Sulphamoil Arginine also contains other interesting chemical elements:. Calcium. (Ca) ...
Carboxypeptidase B, Sequencing Grade Carboxypeptidase B, For Mass Spectrometry
Sequencing Grade Carboxypeptidase B, For Mass Spectrometry from China, Chinas leading Recombinant Carboxypeptidase B product ... Producing high quality Carboxypeptidase B, Sequencing Grade Carboxypeptidase B, For Mass Spectrometry products. ... market, With strict quality control Recombinant Carboxypeptidase B factories, ... Recombinant Carboxypeptidase B Carboxypeptidase Enzyme, Animal Origin Free, Carboxypeptidase B, Proteomics Grade ...
Carboxypeptidases<...
title = "Carboxypeptidases",. abstract = "Carboxypeptidases are involved in a wide range of physiological processes, ranging ... Carboxypeptidases. / Fricker, Lloyd.. xPharm: The Comprehensive Pharmacology Reference. Elsevier Inc., 2007. p. 1-4.. Research ... N2 - Carboxypeptidases are involved in a wide range of physiological processes, ranging from the digestion of food to the ... AB - Carboxypeptidases are involved in a wide range of physiological processes, ranging from the digestion of food to the ...
Investigation of the cellular mechanisms underlying the carboxypeptidase E mutation
Recombinant Human Carboxypeptidase B2/CPB2 (C-6His) | Bon Opus Biosciences
IMSEAR at SEARO: Product stabilization of the two forms of carboxypeptidase A from buffalo pancreas.
The two forms of buffalo carboxypeptidase A lose a part of their activity when incubated at their optimal temperature. Both the ... Sud Mir, Dua R D. Product stabilization of the two forms of carboxypeptidase A from buffalo pancreas. Journal of Biosciences. ... On this basis, a new mechanism for the hydrolysis of acyldipeptides by carboxypeptidase A has been proposed. ... Product stabilization of the two forms of carboxypeptidase A from buffalo pancreas. ...
Kounis Syndrome
Characterization of an islet carboxypeptidase b involved in prohormone processing<...
N2 - An islet carboxypeptidase B-like enzyme (CP B) has been identified and characterized in secretory granules of anglerfish ... AB - An islet carboxypeptidase B-like enzyme (CP B) has been identified and characterized in secretory granules of anglerfish ... An islet carboxypeptidase B-like enzyme (CP B) has been identified and characterized in secretory granules of anglerfish islets ... abstract = "An islet carboxypeptidase B-like enzyme (CP B) has been identified and characterized in secretory granules of ...
Mouse Carboxypeptidase B1 / CPB1 ELISA Pair Set (Artikelnr: SEK50386) von Sino Biological
Mouse Carboxypeptidase B1 / CPB1 ELISA Pair Set detection - quantification SEK50386 Sino Biological Pack: ... Mouse Carboxypeptidase B1 / CPB1 ELISA Pair Set. Brand. Sino Biological. short description. The Mouse Carboxypeptidase B1 / ... The minimum detectable dose of Mouse Carboxypeptidase B1 / CPB1 was determined to be approximately 12.5 pg/ml. This is defined ... The minimum detectable dose of Mouse Carboxypeptidase B1 / CPB1 was determined to be approximately 12.5 pg/ml. This is defined ...
Calcium in PDB 2c6p: Membrane-Bound Glutamate Carboxypeptidase II (Gcpii) in Complex with Phosphate Anion
Membrane-Bound Glutamate Carboxypeptidase II (Gcpii) in Complex with Phosphate Anion ... The structure of Membrane-Bound Glutamate Carboxypeptidase II (Gcpii) in Complex with Phosphate Anion also contains other ... A full contact list of Calcium with other atoms in the Ca binding site number 1 of Membrane-Bound Glutamate Carboxypeptidase II ... Calcium binding site 1 out of 1 in the Membrane-Bound Glutamate Carboxypeptidase II (Gcpii) in Complex with Phosphate Anion. ...
Unprecedented Binding
Mode of Hydroxamate-Based Inhibitors
of Glutamate Carboxypeptidase II: Structural Characterization and...
Unprecedented Binding Mode of Hydroxamate-Based Inhibitors of Glutamate Carboxypeptidase II: Structural Characterization and ... Glutamate Carboxypeptidase IIBiological Activity Inhibitionglutamate carboxypeptidase IIbinding modevivo pharmacokinetics ... Inhibition of glutamate carboxypeptidase II (GCPII) is effective in preclinical models of neurological disorders associated ... Unprecedented Binding Mode of Hydroxamate-Based Inhibitors of Glutamate Carboxypeptidase II: Structural Characterization and ...
PDB 6SN6 | Chain CARBOXYPEPTIDASE T WITH N-SULFAMOYL-L-GLUTAMIC ACID | 6SN6 A | 3D Structure | canSARS
Table of Contents page: Journal of Biological Chemistry
Table 2 - Foodborne Origin and Local and Global Spread of Staphylococcus saprophyticus Causing Human Urinary Tract Infections -...
A Drosophila pattern recognition receptor contains a peptidoglycan docking groove and unusual L,D-carboxypeptidase activity. |...
PDF] Effect of 2-(phosphono-methyl)-pentanedioic acid on allodynia and afferent ectopic discharges in a rat model of...
Inhibition of glutamate carboxypeptidase II (GCP II) reduces glutamate release generated from N-acetyl-aspartyl-glutamate in ... Mice lacking glutamate carboxypeptidase II are protected from peripheral neuropathy and ischemic brain injury. *D. Bacich, K. ... Inhibition of glutamate carboxypeptidase II (GCP II) reduces glutamate release generated from N… Expand. ... The Orally Active Glutamate Carboxypeptidase II Inhibitor E2072 Exhibits Sustained Nerve Exposure and Attenuates Peripheral ...
ENZYMEGCPIISerine carboxypeptidaseArginineProteinHydrolaseProteinsHydrolysisPeptidasesPeptidesReceptorDescriptorProstate CancerInsulinApproximatelyStructureActivityBasicProductMajorSpecificPresentEnzymePancreaticGlutamate carboxypeptidaseProteinsForm a unique fingerprintArginine carboxypeptidasePeptidesEndopeptidasePutativeRecombinantProlyl carboxypeptidaseChymotrypsinCleavesResiduesAntibodyABSTRACTLysineExopeptidasesCPG2ExopeptidaseGlucarpidaseReceptorExtracellularPlasmaHydrolyzesSterile
ENZYME8
- Porcine Carboxypeptidase B Purified Enzyme from Innovative Research has been chromatographically purified. (innov-research.com)
- Carboxypeptidase B is an enzyme involved in the digestion of food. (innov-research.com)
- A protease enzyme, Carboxypeptidase B hydrolyzes peptide bonds at the C-terminal end of a protein or peptide. (innov-research.com)
- Glutamate carboxypeptidase II (GCP II) is a glial enzyme responsible for the hydrolysis of N-acetylaspartylglutamate (NAAG) into glutamate and N-acetylaspartate (NAA). (elsevier.com)
- An islet carboxypeptidase B-like enzyme (CP B) has been identified and characterized in secretory granules of anglerfish islets. (elsevier.com)
- 1,10-Phenanthroline is an inhibitor of metallopeptidases , with one of the first observed instances reported in carboxypeptidase A. [13] Inhibition of the enzyme occurs by removal and chelation of the metal ion required for catalytic activity, leaving an inactive apoenzyme. (wikipedia.org)
- A study was undertaken to determine the effect of inhibition of endogenous carboxypeptidase, neutral endopeptidase, or angiotensin converting enzyme on airway reactivity to the peptide hormone bradykinin. (cdc.gov)
- The authors conclude that airway reactivity to bradykinin is regulated by lung cell membrane carboxypeptidase, endopeptidase, and angiotensin converting enzyme, and that the influence of carboxypeptidase increases when the activities of neutral endopeptidase and angiotensin converting enzyme are reduced. (cdc.gov)
GCPII4
- Glutamate carboxypeptidase II (GCPII) is a 94 kD class II membrane bound zinc metalloenzyme which catalyzes the hydrolysis of the abundant neuropeptide N-acetylaspartylglutamate (NAAG) to glutamate. (jhu.edu)
- Discovery of Orally Available Prodrugs of the Glutamate Carboxypeptidase II (GCPII) Inhibitor 2-Phosphonomethylpentanedioic Acid (2-PMPA). (jhu.edu)
- Inhibition of glutamate carboxypeptidase II (GCPII) is effective in preclinical models of neurological disorders associated with excessive activation of glutamatergic systems. (figshare.com)
- Association of glutamate carboxypeptidase II (GCPII) haplotypes with breast and prostate cancer risk. (cdc.gov)
Serine carboxypeptidase1
- Kex1p is a prohormone-processing serine carboxypeptidase found in Saccharomyces cerevisiae. (rcsb.org)
Arginine4
- The binding sites of Zinc atom in the Carboxypeptidase T with Sulphamoil Arginine (pdb code 4gm5 ). (atomistry.com)
- Carboxypeptidase T with Sulphamoil Arginine To Be Published . (atomistry.com)
- Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. (recombinanttrypsin.com)
- Carboxypeptidase B is competitively inhibited by arginine and lysine. (recombinanttrypsin.com)
Protein2
- The protein, known as CPE-delta N, is a form of carboxypeptidase E (CPE). (nih.gov)
- Penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase [Interproscan]. (ntu.edu.sg)
Hydrolase2
- The spherical carboxypeptidase domain (first 295 amino acids) is arranged in a typical α/β hydrolase fold and carries the catalytic site. (atlasgeneticsoncology.org)
- Comparison of Substrate Specificity of Escherichia Coli p -Aminobenzoyl-Glutamate Hydrolase with Pseudomonas Carboxypeptidase G. (bvsalud.org)
Proteins1
- Carboxypeptidases help proteins to mature and are involved in regulating biological processes, and can be found helping with various functions like blood clotting, producing growth factor, wound healing, and more. (innov-research.com)
Hydrolysis1
- On this basis, a new mechanism for the hydrolysis of acyldipeptides by carboxypeptidase A has been proposed. (who.int)
Peptidases1
- Lung peptidases, including carboxypeptidase, modulate airway reactivity to intravenous bradykinin. (cdc.gov)
Peptides1
- Carboxypeptidases are involved in a wide range of physiological processes, ranging from the digestion of food to the biosynthesis of peptides that function in cell-cell signaling. (elsevier.com)
Receptor1
- A Drosophila pattern recognition receptor contains a peptidoglycan docking groove and unusual L,D-carboxypeptidase activity. (sinica.edu.tw)
Descriptor1
- Muramoylpentapeptide Carboxypeptidase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (rush.edu)
Prostate Cancer2
- Barinka C, Rojas C, Slusher BS, Pomper M. "Glutamate carboxypeptidase II in diagnosis and treatment of neurologic disorders and prostate cancer. (jhu.edu)
- Structure of Glutamate Carboxypeptidase II, A Drug Target in Neuronal Damage and Prostate Cancer. (atomistry.com)
Insulin1
- Carboxypeptidase E (CPE) is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. (elsevier.com)
Approximately1
- The minimum detectable dose of Mouse Carboxypeptidase B1 / CPB1 was determined to be approximately 12.5 pg/ml. (elisa-kits.de)
Structure2
- The CPM structure consists of two domains, the classical carboxypeptidase domain and the C-terminal domain. (atlasgeneticsoncology.org)
- The catalytic carboxypeptidase domain is shown on top and the cup-shaped C-terminal domain on bottom of the structure. (atlasgeneticsoncology.org)
Activity1
- The two forms of buffalo carboxypeptidase A lose a part of their activity when incubated at their optimal temperature. (who.int)
Basic1
- CPM is a basic metallo-carboxypeptidase. (atlasgeneticsoncology.org)
Product2
Major1
- This graph shows the total number of publications written about "Muramoylpentapeptide Carboxypeptidase" by people in this website by year, and whether "Muramoylpentapeptide Carboxypeptidase" was a major or minor topic of these publications. (rush.edu)
Specific1
- It utilizes a monoclonal antibody specific for Carboxypeptidase B1 / CPB1 coated on a 96-well plate. (elisa-kits.de)
Present2
- Standards and samples are added to the wells, and any Carboxypeptidase B1 / CPB1 present binds to the immobilized antibody. (elisa-kits.de)
- The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Carboxypeptidase B1 / CPB1 present in the sample. (elisa-kits.de)
Enzyme11
- The crystal structures of carboxypeptidase T (CpT) complexes with phenylalanine and arginine substrate analogs - benzylsuccinic acid and (2-guanidinoethylmercapto)succinic acid - were determined by the molecular replacement method at resolutions of 1.57 Å and 1.62 Å to clarify the broad substrate specificity profile of the enzyme. (sigmaaldrich.com)
- This study examined the effect of 2-(phosphonomethyl)-pentanedioic acid (2-PMPA), a potent and selective inhibitor of glutamate carboxypeptidase II (GCP II), an enzyme releasing glutamate and N-acetyl-aspartate from synaptical terminals, on the electroconvulsive threshold in mice. (unboundmedicine.com)
- Identification of prolyl carboxypeptidase as an alternative enzyme for processing of renal angiotensin II using mass spectrometry. (nih.gov)
- galactosidase and carboxypeptidase A. The purified enzyme liberated sialic acid residues from sialooligosaccaharides (a-2,3- was preferred than a-2,6-sialyllactose), sialoglycoprotein and ganglioside such as GM3 and GD1a, however, the GM2 and GD1b are not suitable substrates for the sialidase as were also shown for BSM and guinea pig serum. (unmul.ac.id)
- Pancreatic or tissue Carboxypeptidase B (CPB), a key enzyme involved in insulin conversion and highly specific for excising C-terminal Lys and Arg residues from peptides and proteins, was expressed at high level and purified from a recombinant Pichia pastoris strain. (recombinanttrypsin.com)
- There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. (recombinanttrypsin.com)
- The enzyme is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, folypoly-gamma-glutamate carboxypeptidase (FGCP), and N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu to produce glutamate, another neurotransmitter. (rndsystems.com)
- Prolyl carboxypeptidase purified from human placenta: its characterization and identification as an apelin-cleaving enzyme [electronic resource]. (usda.gov)
- A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9. (invivogen.com)
- Identification of Carboxypeptidase N as the plasma enzyme responsible for C-terminal cleavage of stromal cell-derived factor-1 alpha. (nih.gov)
- Importance: COVID-19 is caused by SARS-CoV-2, a betacoronavirus that uses the angiotensin-converting enzyme-related carboxypeptidase (ACE2) receptor to gain entry into cells. (unav.edu)
Pancreatic1
- A cDNA containing the porcine pancreatic pro-Carboxypeptidase B (proCPB) fused to the Saccharomyces cerevisiae alpha factor secretion signal was cloned into the pPIC3K vector under control of P. pastoris AOX1 promoter. (recombinanttrypsin.com)
Glutamate carboxypeptidase6
- 2-PMPA is a potent and selective inhibitor of glutamate carboxypeptidase II (GCPII) (IC50=300 pM). (cymitquimica.com)
- 2-Phosphonomethylpentanedioic acid (1, 2-PMPA) is a potent inhibitor of glutamate carboxypeptidase II which has demonstrated robust neuroprotective efficacy in many neurological disease models. (elsevier.com)
- Glutamate Carboxypeptidase II in Aging Rat Prefrontal Cortex Impairs Working Memory Performance. (yale.edu)
- Prostate-specific membrane antigen (PSMA), also known as glutamate carboxypeptidase II (GCPII), is an important diagnostic and therapeutic target in prostate cancer. (urotoday.com)
- Relations of glutamate carboxypeptidase II (GCPII) polymorphisms to folate and homocysteine concentrations and to scores of cognition, anxiety, and depression in a homogeneous Norwegian population: the Hordaland Homocysteine Study. (uib.no)
- Association of glutamate carboxypeptidase II (GCPII) haplotypes with breast and prostate cancer risk. (cdc.gov)
Proteins2
- Combined proteomics and transcriptomics identifies carboxypeptidase B1 and nuclear factor ?B (NF-?B) associated proteins as putative biomarkers of metastasis in low grade breast cancer, Mol. (muni.cz)
- Carboxypeptidase which preferentially cleaves C-terminal acidic residues from peptides and proteins. (nih.gov)
Form a unique fingerprint1
- Dive into the research topics of 'Methods for studying carboxypeptidase E'. Together they form a unique fingerprint. (elsevier.com)
Arginine carboxypeptidase1
- arginine carboxypeptidase. (expasy.org)
Peptides1
- Carboxypeptidase B2 preferentially releases the C-terminal amino acids Arg and Lys from biologically active peptides such as Kinins and Anaphylatoxins thus rendering them inactive. (abbiotec.com)
Endopeptidase3
- Furthermore, the dual prolyl carboxypeptidase (PCP)-prolyl endopeptidase (PEP) inhibitor Z-prolyl-prolinal reduced ANG-(1-7) formation in ACE2 KO mice, while the ACE2 inhibitor MLN-4760 had no effect. (nih.gov)
- In fact, the proteases have endopeptidase as well as exopeptidase (amino and carboxypeptidase) activities. (healthynewage.com)
- It possesses both endopeptidase and dipeptidyl carboxypeptidase activites. (immunochemistry.com)
Putative2
- We have generated a molecular model of CPE, and structural alignment with other carboxypeptidases revealed a putative sorting signal binding domain that is independent of the enzymatic site. (nih.gov)
- Four mutants were killed exclusively by lysozyme and not other cell wall-targeting molecules, including the peptidoglycan deacetylase encoded by pgdA, the putative carboxypeptidase encoded by pbpX, the orphan response regulator encoded by degU, and the highly abundant noncoding RNA encoded by rli31. (pasteur.fr)
Recombinant6
- Recombinant Carboxypeptidase B, ≥170 USP Units/Mg Pro. (recombinanttrypsin.com)
- Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. (recombinanttrypsin.com)
- Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage. (recombinanttrypsin.com)
- 1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level. (recombinanttrypsin.com)
- Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. (recombinanttrypsin.com)
- VORAXAZE (glucarpidase) is a carboxypeptidase produced by recombinant DNA technology in genetically modified Escherichia coli . (druglib.com)
Prolyl carboxypeptidase1
- Deletion of prolyl carboxypeptidase attenuates the metabolic effects o" by Jin Kwon Jeong, Gyorgyi Szabo et al. (gwu.edu)
Chymotrypsin1
- In this report the general principles and potential application of affinity chro- matography are illustrated by results of its application to the purification of staphy- lococcal nuclease, a-chymotrypsin, and carboxypeptidase A. The solid matrix used in these studies was Sepharose (a "beaded" form of the cross-linked dextran of highly porous structure, agarose®) which displays virtually all the desirable fea- tures listed above. (nih.gov)
Cleaves1
- Carboxypeptidase-B sequentially cleaves C terminal K and R residues. (recombinanttrypsin.com)
Residues1
- The conservative Leu211 and Leu254 residues (also present in both carboxypeptidase A and carboxypeptidase B) were shown to be structural determinants for recognition of hydrophobic substrates, whereas Asp263 was for recognition of positively charged substrates. (sigmaaldrich.com)
Antibody1
- After overnight incubation, cells were stained using the Triton X100 fix/perm protocol (see Recommended Assay Procedure) and the anti-Carboxypeptidase E antibody. (bdbiosciences.com)
ABSTRACT1
- abstract = "This chapter discusses the methods for studying carboxypeptidase E (CPE). (elsevier.com)
Lysine3
- lysine carboxypeptidase. (expasy.org)
- Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. (recombinanttrypsin.com)
- Carboxypeptidase B is competitively inhibited by arginine and lysine. (recombinanttrypsin.com)
Exopeptidases1
- Carboxypeptidases are zinc-containing exopeptidases that catalyze the release of carboxy-terminal amino acids, and are synthesized as zymogens that are activated by proteolytic cleavage. (nih.gov)
CPG21
- carboxypeptidase G2 (CPG2), which hydrolyzes C-terminal glutamate-masking groups. (scienceboard.net)
Exopeptidase1
- Carboxypeptidase E (CPE) also known as Neurotrophic factor-α1 (NF-α1), was first identified as an exopeptidase and is highly expressed in the nervous and endocrine systems. (nih.gov)
Glucarpidase1
- 5. Glucarpidase (carboxypeptidase g2) intervention in adult and elderly cancer patients with renal dysfunction and delayed methotrexate elimination after high-dose methotrexate therapy. (nih.gov)
Receptor1
- The membrane-bound form of Carboxypeptidase E (CPE) was identified as a sorting receptor for the regulated secretory pathway through studies carried out in our lab. (nih.gov)
Extracellular2
- A transmembrane glycoprotein with an extracellular catalytic domain which functions as a carboxypeptidase. (nih.gov)
- This study highlights the role of BRS1 in plant redox regulation and stress responses, which extends our understanding of extracellular serine carboxypeptidases. (researchsquare.com)
Plasma1
- VORAXAZE is a carboxypeptidase indicated to reduce toxic plasma methotrexate concentration (greater than 1 micromole per liter) in adult and pediatric patients with delayed methotrexate clearance (plasma methotrexate concentrations greater than 2 standard deviations of the mean methotrexate excretion curve specific for the dose of methotrexate administered) due to impaired renal function. (nih.gov)
Hydrolyzes1
- Some of the vancomycin resistance operons encode VanXY D,D-carboxypeptidase, which hydrolyzes both di- and pentapeptide. (rcsb.org)
Sterile1
- Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. (recombinanttrypsin.com)