Carbon: A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.Glutamine: A non-essential amino acid present abundantly throughout the body and is involved in many metabolic processes. It is synthesized from GLUTAMIC ACID and AMMONIA. It is the principal carrier of NITROGEN in the body and is an important energy source for many cells.Nitrogen: An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.Amides: Organic compounds containing the -CO-NH2 radical. Amides are derived from acids by replacement of -OH by -NH2 or from ammonia by the replacement of H by an acyl group. (From Grant & Hackh's Chemical Dictionary, 5th ed)Ubiquitin-Protein Ligases: A diverse class of enzymes that interact with UBIQUITIN-CONJUGATING ENZYMES and ubiquitination-specific protein substrates. Each member of this enzyme group has its own distinct specificity for a substrate and ubiquitin-conjugating enzyme. Ubiquitin-protein ligases exist as both monomeric proteins multiprotein complexes.Glutamate-Ammonia Ligase: An enzyme that catalyzes the conversion of ATP, L-glutamate, and NH3 to ADP, orthophosphate, and L-glutamine. It also acts more slowly on 4-methylene-L-glutamate. (From Enzyme Nomenclature, 1992) EC 6.3.1.2.DNA Ligases: Poly(deoxyribonucleotide):poly(deoxyribonucleotide)ligases. Enzymes that catalyze the joining of preformed deoxyribonucleotides in phosphodiester linkage during genetic processes during repair of a single-stranded break in duplex DNA. The class includes both EC 6.5.1.1 (ATP) and EC 6.5.1.2 (NAD).Tissue Donors: Individuals supplying living tissue, organs, cells, blood or blood components for transfer or transplantation to histocompatible recipients.SKP Cullin F-Box Protein Ligases: A subset of ubiquitin protein ligases that are formed by the association of a SKP DOMAIN PROTEIN, a CULLIN DOMAIN PROTEIN and a F-BOX DOMAIN PROTEIN.Nitrogen Fixation: The process in certain BACTERIA; FUNGI; and CYANOBACTERIA converting free atmospheric NITROGEN to biologically usable forms of nitrogen, such as AMMONIA; NITRATES; and amino compounds.Carbon Dioxide: A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals.Nitrogen Isotopes: Stable nitrogen atoms that have the same atomic number as the element nitrogen, but differ in atomic weight. N-15 is a stable nitrogen isotope.Carbon Monoxide: Carbon monoxide (CO). A poisonous colorless, odorless, tasteless gas. It combines with hemoglobin to form carboxyhemoglobin, which has no oxygen carrying capacity. The resultant oxygen deprivation causes headache, dizziness, decreased pulse and respiratory rates, unconsciousness, and death. (From Merck Index, 11th ed)Blood DonorsNanotubes, Carbon: Nanometer-sized tubes composed mainly of CARBON. Such nanotubes are used as probes for high-resolution structural and chemical imaging of biomolecules with ATOMIC FORCE MICROSCOPY.Ammonia: A colorless alkaline gas. It is formed in the body during decomposition of organic materials during a large number of metabolically important reactions. Note that the aqueous form of ammonia is referred to as AMMONIUM HYDROXIDE.Living Donors: Non-cadaveric providers of organs for transplant to related or non-related recipients.GlutaminaseCullin Proteins: A family of structurally related proteins that were originally discovered for their role in cell-cycle regulation in CAENORHABDITIS ELEGANS. They play important roles in regulation of the CELL CYCLE and as components of UBIQUITIN-PROTEIN LIGASES.Ubiquitination: The act of ligating UBIQUITINS to PROTEINS to form ubiquitin-protein ligase complexes to label proteins for transport to the PROTEASOME ENDOPEPTIDASE COMPLEX where proteolysis occurs.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Nitrogen Compounds: Inorganic compounds that contain nitrogen as an integral part of the molecule.Carbon Isotopes: Stable carbon atoms that have the same atomic number as the element carbon, but differ in atomic weight. C-13 is a stable carbon isotope.Polynucleotide Ligases: Catalyze the joining of preformed ribonucleotides or deoxyribonucleotides in phosphodiester linkage during genetic processes. EC 6.5.1.Nitrogen Cycle: The circulation of nitrogen in nature, consisting of a cycle of biochemical reactions in which atmospheric nitrogen is compounded, dissolved in rain, and deposited in the soil, where it is assimilated and metabolized by bacteria and plants, eventually returning to the atmosphere by bacterial decomposition of organic matter.Ubiquitin: A highly conserved 76-amino acid peptide universally found in eukaryotic cells that functions as a marker for intracellular PROTEIN TRANSPORT and degradation. Ubiquitin becomes activated through a series of complicated steps and forms an isopeptide bond to lysine residues of specific proteins within the cell. These "ubiquitinated" proteins can be recognized and degraded by proteosomes or be transported to specific compartments within the cell.Blood Urea Nitrogen: The urea concentration of the blood stated in terms of nitrogen content. Serum (plasma) urea nitrogen is approximately 12% higher than blood urea nitrogen concentration because of the greater protein content of red blood cells. Increases in blood or serum urea nitrogen are referred to as azotemia and may have prerenal, renal, or postrenal causes. (From Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)Nitrogen Dioxide: Nitrogen oxide (NO2). A highly poisonous gas. Exposure produces inflammation of lungs that may only cause slight pain or pass unnoticed, but resulting edema several days later may cause death. (From Merck, 11th ed) It is a major atmospheric pollutant that is able to absorb UV light that does not reach the earth's surface.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.Coenzyme A Ligases: Enzymes that catalyze the formation of acyl-CoA derivatives. EC 6.2.1.RING Finger Domains: A zinc-binding domain defined by the sequence Cysteine-X2-Cysteine-X(9-39)-Cysteine-X(l-3)-His-X(2-3)-Cysteine-X2-Cysteine -X(4-48)-Cysteine-X2-Cysteine, where X is any amino acid. The RING finger motif binds two atoms of zinc, with each zinc atom ligated tetrahedrally by either four cysteines or three cysteines and a histidine. The motif also forms into a unitary structure with a central cross-brace region and is found in many proteins that are involved in protein-protein interactions. The acronym RING stands for Really Interesting New Gene.Methionine SulfoximineCarbon Monoxide Poisoning: Toxic asphyxiation due to the displacement of oxygen from oxyhemoglobin by carbon monoxide.Kinetics: The rate dynamics in chemical or physical systems.Ubiquitin-Conjugating Enzymes: A class of enzymes that form a thioester bond to UBIQUITIN with the assistance of UBIQUITIN-ACTIVATING ENZYMES. They transfer ubiquitin to the LYSINE of a substrate protein with the assistance of UBIQUITIN-PROTEIN LIGASES.Reactive Nitrogen Species: Nitrogenous products of NITRIC OXIDE synthases, ranging from NITRIC OXIDE to NITRATES. These reactive nitrogen intermediates also include the inorganic PEROXYNITROUS ACID and the organic S-NITROSOTHIOLS.Glutamates: Derivatives of GLUTAMIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the 2-aminopentanedioic acid structure.PII Nitrogen Regulatory Proteins: A family of signal transducing adaptor proteins that control the METABOLISM of NITROGEN. They are primarily found in prokaryotes.Diazooxonorleucine: An amino acid that inhibits phosphate-activated glutaminase and interferes with glutamine metabolism. It is an antineoplastic antibiotic produced by an unidentified species of Streptomyces from Peruvian soil. (From Merck Index, 11th ed)Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Glutamate Synthase: An enzyme that catalyzes the formation of 2 molecules of glutamate from glutamine plus alpha-ketoglutarate in the presence of NADPH. EC 1.4.1.13.Quaternary Ammonium Compounds: Derivatives of ammonium compounds, NH4+ Y-, in which all four of the hydrogens bonded to nitrogen have been replaced with hydrocarbyl groups. These are distinguished from IMINES which are RN=CR2.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.RNA Ligase (ATP): An enzyme that catalyzes the conversion of linear RNA to a circular form by the transfer of the 5'-phosphate to the 3'-hydroxyl terminus. It also catalyzes the covalent joining of two polyribonucleotides in phosphodiester linkage. EC 6.5.1.3.Glutamic Acid: A non-essential amino acid naturally occurring in the L-form. Glutamic acid is the most common excitatory neurotransmitter in the CENTRAL NERVOUS SYSTEM.Magnetic Resonance Spectroscopy: Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).AmidohydrolasesNitrogen Oxides: Inorganic oxides that contain nitrogen.Nitrates: Inorganic or organic salts and esters of nitric acid. These compounds contain the NO3- radical.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Urea: A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Carbon Tetrachloride: A solvent for oils, fats, lacquers, varnishes, rubber waxes, and resins, and a starting material in the manufacturing of organic compounds. Poisoning by inhalation, ingestion or skin absorption is possible and may be fatal. (Merck Index, 11th ed)Bacterial Proteins: Proteins found in any species of bacterium.Ketoglutaric Acids: A family of compounds containing an oxo group with the general structure of 1,5-pentanedioic acid. (From Lehninger, Principles of Biochemistry, 1982, p442)Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Carbon Sequestration: Any of several processes for the permanent or long-term artificial or natural capture or removal and storage of carbon dioxide and other forms of carbon, through biological, chemical or physical processes, in a manner that prevents it from being released into the atmosphere.Amide Synthases: Enzymes that catalyze the joining of either ammonia or an amide with another molecule, in which the linkage is in the form of a carbon-nitrogen bond. EC 6.3.1.Alanine: A non-essential amino acid that occurs in high levels in its free state in plasma. It is produced from pyruvate by transamination. It is involved in sugar and acid metabolism, increases IMMUNITY, and provides energy for muscle tissue, BRAIN, and the CENTRAL NERVOUS SYSTEM.F-Box Proteins: A family of proteins that share the F-BOX MOTIF and are involved in protein-protein interactions. They play an important role in process of protein ubiquition by associating with a variety of substrates and then associating into SCF UBIQUITIN LIGASE complexes. They are held in the ubiquitin-ligase complex via binding to SKP DOMAIN PROTEINS.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Catalysis: The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Endosomal Sorting Complexes Required for Transport: A set of protein subcomplexes involved in PROTEIN SORTING of UBIQUITINATED PROTEINS into intraluminal vesicles of MULTIVESICULAR BODIES and in membrane scission during formation of intraluminal vesicles, during the final step of CYTOKINESIS, and during the budding of enveloped viruses. The ESCRT machinery is comprised of the protein products of Class E vacuolar protein sorting genes.Glutamate Dehydrogenase: An enzyme that catalyzes the conversion of L-glutamate and water to 2-oxoglutarate and NH3 in the presence of NAD+. (From Enzyme Nomenclature, 1992) EC 1.4.1.2.Glucose: A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.Ubiquitins: A family of proteins that are structurally-related to Ubiquitin. Ubiquitins and ubiquitin-like proteins participate in diverse cellular functions, such as protein degradation and HEAT-SHOCK RESPONSE, by conjugation to other proteins.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Proteasome Endopeptidase Complex: A large multisubunit complex that plays an important role in the degradation of most of the cytosolic and nuclear proteins in eukaryotic cells. It contains a 700-kDa catalytic sub-complex and two 700-kDa regulatory sub-complexes. The complex digests ubiquitinated proteins and protein activated via ornithine decarboxylase antizyme.Carbon Disulfide: A colorless, flammable, poisonous liquid, CS2. It is used as a solvent, and is a counterirritant and has local anesthetic properties but is not used as such. It is highly toxic with pronounced CNS, hematologic, and dermatologic effects.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Biomass: Total mass of all the organisms of a given type and/or in a given area. (From Concise Dictionary of Biology, 1990) It includes the yield of vegetative mass produced from any given crop.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Structure-Activity Relationship: The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Fertilizers: Substances or mixtures that are added to the soil to supply nutrients or to make available nutrients already present in the soil, in order to increase plant growth and productivity.Peptide Synthases: Ligases that catalyze the joining of adjacent AMINO ACIDS by the formation of carbon-nitrogen bonds between their carboxylic acid groups and amine groups.Soil: The unconsolidated mineral or organic matter on the surface of the earth that serves as a natural medium for the growth of land plants.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.Asparagine: A non-essential amino acid that is involved in the metabolic control of cell functions in nerve and brain tissue. It is biosynthesized from ASPARTIC ACID and AMMONIA by asparagine synthetase. (From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed)Aspartic Acid: One of the non-essential amino acids commonly occurring in the L-form. It is found in animals and plants, especially in sugar cane and sugar beets. It may be a neurotransmitter.Hydrogen: The first chemical element in the periodic table. It has the atomic symbol H, atomic number 1, and atomic weight [1.00784; 1.00811]. It exists, under normal conditions, as a colorless, odorless, tasteless, diatomic gas. Hydrogen ions are PROTONS. Besides the common H1 isotope, hydrogen exists as the stable isotope DEUTERIUM and the unstable, radioactive isotope TRITIUM.Adenosine Monophosphate: Adenine nucleotide containing one phosphate group esterified to the sugar moiety in the 2'-, 3'-, or 5'-position.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Polyubiquitin: An oligomer formed from the repetitive linking of the C-terminal glycine of one UBIQUITIN molecule via an isopeptide bond to a lysine residue on a second ubiquitin molecule. It is structurally distinct from UBIQUITIN C, which is a single protein containing a tandemly arrayed ubiquitin peptide sequence.Lysine: An essential amino acid. It is often added to animal feed.Ubiquitin-Protein Ligase Complexes: Complexes of enzymes that catalyze the covalent attachment of UBIQUITIN to other proteins by forming a peptide bond between the C-terminal GLYCINE of UBIQUITIN and the alpha-amino groups of LYSINE residues in the protein. The complexes play an important role in mediating the selective-degradation of short-lived and abnormal proteins. The complex of enzymes can be broken down into three components that involve activation of ubiquitin (UBIQUITIN-ACTIVATING ENZYMES), conjugation of ubiquitin to the ligase complex (UBIQUITIN-CONJUGATING ENZYMES), and ligation of ubiquitin to the substrate protein (UBIQUITIN-PROTEIN LIGASES).Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Carbon Radioisotopes: Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.Citric Acid Cycle: A series of oxidative reactions in the breakdown of acetyl units derived from GLUCOSE; FATTY ACIDS; or AMINO ACIDS by means of tricarboxylic acid intermediates. The end products are CARBON DIOXIDE, water, and energy in the form of phosphate bonds.Nitrogenase: An enzyme system that catalyzes the fixing of nitrogen in soil bacteria and blue-green algae (CYANOBACTERIA). EC 1.18.6.1.Nitrogenous Group Transferases: Enzymes that catalyze the transfer of nitrogenous groups, primarily amino groups, from a donor, generally an amino acid, to an acceptor, usually a 2-oxoacid. EC 2.6.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Transaminases: A subclass of enzymes of the transferase class that catalyze the transfer of an amino group from a donor (generally an amino acid) to an acceptor (generally a 2-keto acid). Most of these enzymes are pyridoxyl phosphate proteins. (Dorland, 28th ed) EC 2.6.1.Arginine: An essential amino acid that is physiologically active in the L-form.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Plant Roots: The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)Saccharomyces cerevisiae Proteins: Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.Symbiosis: The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Ubiquitin-Activating Enzymes: A class of enzymes that catalyzes the ATP-dependent formation of a thioester bond between itself and UBIQUITIN. It then transfers the activated ubiquitin to one of the UBIQUITIN-PROTEIN LIGASES.Amidophosphoribosyltransferase: An enzyme, involved in the early steps of purine nucleotide biosynthesis, that catalyzes the formation of 5-phosphoribosylamine from glutamine and phosphoribosylpyrophosphate. EC 2.4.2.14.Carbon-Oxygen Ligases: Enzymes that catalyze the joining of two molecules by the formation of a carbon-oxygen bond. EC 6.1.Carbon Tetrachloride PoisoningStereoisomerism: The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Acetates: Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Photosynthesis: The synthesis by organisms of organic chemical compounds, especially carbohydrates, from carbon dioxide using energy obtained from light rather than from the oxidation of chemical compounds. Photosynthesis comprises two separate processes: the light reactions and the dark reactions. In higher plants; GREEN ALGAE; and CYANOBACTERIA; NADPH and ATP formed by the light reactions drive the dark reactions which result in the fixation of carbon dioxide. (from Oxford Dictionary of Biochemistry and Molecular Biology, 2001)Proteolysis: Cleavage of proteins into smaller peptides or amino acids either by PROTEASES or non-enzymatically (e.g., Hydrolysis). It does not include Protein Processing, Post-Translational.Plant Leaves: Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)Genes, Bacterial: The functional hereditary units of BACTERIA.Adenosine Triphosphate: An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.Oxygen: An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.Proto-Oncogene Proteins c-cbl: Proto-oncogene proteins that negatively regulate RECEPTOR PROTEIN-TYROSINE KINASE signaling. It is a UBIQUITIN-PROTEIN LIGASE and the cellular homologue of ONCOGENE PROTEIN V-CBL.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Leucine: An essential branched-chain amino acid important for hemoglobin formation.Water: A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Models, Chemical: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Protein Structure, Secondary: The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.Ammonium Chloride: An acidifying agent that has expectorant and diuretic effects. Also used in etching and batteries and as a flux in electroplating.Mass Spectrometry: An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Mutagenesis, Site-Directed: Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.Deuterium: Deuterium. The stable isotope of hydrogen. It has one neutron and one proton in the nucleus.Glucagon-Like Peptides: Peptides derived from proglucagon which is also the precursor of pancreatic GLUCAGON. Despite expression of proglucagon in multiple tissues, the major production site of glucagon-like peptides (GLPs) is the INTESTINAL L CELLS. GLPs include glucagon-like peptide 1, glucagon-like peptide 2, and the various truncated forms.Hydrogen Bonding: A low-energy attractive force between hydrogen and another element. It plays a major role in determining the properties of water, proteins, and other compounds.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Polyunsaturated Alkamides: Amides composed of unsaturated aliphatic FATTY ACIDS linked with AMINES by an amide bond. They are most prominent in ASTERACEAE; PIPERACEAE; and RUTACEAE; and also found in ARISTOLOCHIACEAE; BRASSICACEAE; CONVOLVULACEAE; EUPHORBIACEAE; MENISPERMACEAE; POACEAE; and SOLANACEAE. They are recognized by their pungent taste and for causing numbing and salivation.Ornithine: An amino acid produced in the urea cycle by the splitting off of urea from arginine.Dipeptides: Peptides composed of two amino acid units.Hydrolysis: The process of cleaving a chemical compound by the addition of a molecule of water.Atmosphere: The gaseous envelope surrounding a planet or similar body. (From Random House Unabridged Dictionary, 2d ed)Carbohydrate Metabolism: Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.Parenteral Nutrition, Total: The delivery of nutrients for assimilation and utilization by a patient whose sole source of nutrients is via solutions administered intravenously, subcutaneously, or by some other non-alimentary route. The basic components of TPN solutions are protein hydrolysates or free amino acid mixtures, monosaccharides, and electrolytes. Components are selected for their ability to reverse catabolism, promote anabolism, and build structural proteins.Molecular Conformation: The characteristic three-dimensional shape of a molecule.Tissue and Organ Procurement: The administrative procedures involved with acquiring TISSUES or organs for TRANSPLANTATION through various programs, systems, or organizations. These procedures include obtaining consent from TISSUE DONORS and arranging for transportation of donated tissues and organs, after TISSUE HARVESTING, to HOSPITALS for processing and transplantation.S-Phase Kinase-Associated Proteins: A family of structurally-related proteins that were originally identified by their ability to complex with cyclin proteins (CYCLINS). They share a common domain that binds specifically to F-BOX MOTIFS. They take part in SKP CULLIN F-BOX PROTEIN LIGASES, where they can bind to a variety of F-BOX PROTEINS.Endocannabinoids: Fatty acid derivatives that have specificity for CANNABINOID RECEPTORS. They are structurally distinct from CANNABINOIDS and were originally discovered as a group of endogenous CANNABINOID RECEPTOR AGONISTS.Protein Inhibitors of Activated STAT: A family of structurally related proteins that are constitutively expressed and that negatively regulate cytokine-mediated SIGNAL TRANSDUCTION PATHWAYS. PIAS proteins inhibit the activity of signal transducers and activators of transcription.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.AcetylenePhenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Cyanobacteria: A phylum of oxygenic photosynthetic bacteria comprised of unicellular to multicellular bacteria possessing CHLOROPHYLL a and carrying out oxygenic PHOTOSYNTHESIS. Cyanobacteria are the only known organisms capable of fixing both CARBON DIOXIDE (in the presence of light) and NITROGEN. Cell morphology can include nitrogen-fixing heterocysts and/or resting cells called akinetes. Formerly called blue-green algae, cyanobacteria were traditionally treated as ALGAE.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Amino Acid Motifs: Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.Plants: Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.Histidine: An essential amino acid that is required for the production of HISTAMINE.SUMO-1 Protein: A 1.5-kDa small ubiquitin-related modifier protein that can covalently bind via an isopeptide link to a number of cellular proteins. It may play a role in intracellular protein transport and a number of other cellular processes.Protons: Stable elementary particles having the smallest known positive charge, found in the nuclei of all elements. The proton mass is less than that of a neutron. A proton is the nucleus of the light hydrogen atom, i.e., the hydrogen ion.Small Ubiquitin-Related Modifier Proteins: A class of structurally related proteins of 12-20 kDa in size. They covalently modify specific proteins in a manner analogous to UBIQUITIN.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.RNA, Transfer, Gln: A transfer RNA which is specific for carrying glutamine to sites on the ribosomes in preparation for protein synthesis.Proline: A non-essential amino acid that is synthesized from GLUTAMIC ACID. It is an essential component of COLLAGEN and is important for proper functioning of joints and tendons.Nitrogen Mustard Compounds: A group of alkylating agents derived from mustard gas, with the sulfur replaced by nitrogen. They were formerly used as toxicants and vesicants, but now function as antineoplastic agents. These compounds are also powerful mutagens, teratogens, immunosuppressants, and carcinogens.Ecosystem: A functional system which includes the organisms of a natural community together with their environment. (McGraw Hill Dictionary of Scientific and Technical Terms, 4th ed)Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Catalytic Domain: The region of an enzyme that interacts with its substrate to cause the enzymatic reaction.Fermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing): An enzyme that catalyzes the synthesis of fructose-6-phosphate plus GLUTAMINE from GLUTAMATE plus glucosamine-6-phosphate.Amino Acid Transport Systems, Neutral: Amino acid transporter systems capable of transporting neutral amino acids (AMINO ACIDS, NEUTRAL).Metabolic Networks and Pathways: Complex sets of enzymatic reactions connected to each other via their product and substrate metabolites.Enzyme Inhibitors: Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.Carbon-Nitrogen Ligases: Enzymes that catalyze the joining of two molecules by the formation of a carbon-nitrogen bond. EC 6.3.Transplantation, Homologous: Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals.Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.Anthranilate Synthase: An enzyme that catalyzes the formation of anthranilate (o-aminobenzoate) and pyruvic acid from chorismate and glutamine. Anthranilate is the biosynthetic precursor of tryptophan and numerous secondary metabolites, including inducible plant defense compounds. EC 4.1.3.27.Carbon Footprint: A measure of the total greenhouse gas emissions produced by an individual, organization, event, or product. It is measured in units of equivalent kilograms of CARBON DIOXIDE generated in a given time frame.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Graft Survival: The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Repressor Proteins: Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.Carbamates: Derivatives of carbamic acid, H2NC(=O)OH. Included under this heading are N-substituted and O-substituted carbamic acids. In general carbamate esters are referred to as urethanes, and polymers that include repeating units of carbamate are referred to as POLYURETHANES. Note however that polyurethanes are derived from the polymerization of ISOCYANATES and the singular term URETHANE refers to the ethyl ester of carbamic acid.Glycine: A non-essential amino acid. It is found primarily in gelatin and silk fibroin and used therapeutically as a nutrient. It is also a fast inhibitory neurotransmitter.Enzyme Repression: The interference in synthesis of an enzyme due to the elevated level of an effector substance, usually a metabolite, whose presence would cause depression of the gene responsible for enzyme synthesis.Spectroscopy, Fourier Transform Infrared: A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.Spectrophotometry, Infrared: Spectrophotometry in the infrared region, usually for the purpose of chemical analysis through measurement of absorption spectra associated with rotational and vibrational energy levels of molecules. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Nitrate Reductase: An enzyme that catalyzes the oxidation of nitrite to nitrate. It is a cytochrome protein that contains IRON and MOLYBDENUM.Trees: Woody, usually tall, perennial higher plants (Angiosperms, Gymnosperms, and some Pterophyta) having usually a main stem and numerous branches.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Gene Expression Regulation, Enzymologic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.Gene Expression Regulation, Plant: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.Histidine Ammonia-Lyase: An enzyme that catalyzes the first step of histidine catabolism, forming UROCANIC ACID and AMMONIA from HISTIDINE. Deficiency of this enzyme is associated with elevated levels of serum histidine and is called histidinemia (AMINO ACID METABOLISM, INBORN ERRORS).Nuclear Magnetic Resonance, Biomolecular: NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.Parenteral Nutrition: The administering of nutrients for assimilation and utilization by a patient who cannot maintain adequate nutrition by enteral feeding alone. Nutrients are administered by a route other than the alimentary canal (e.g., intravenously, subcutaneously).Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Gene Expression Regulation, Fungal: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.Protein Processing, Post-Translational: Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.

Three asparagine synthetase genes of Bacillus subtilis. (1/39)

Three asparagine synthetase genes, asnB, asnH, and asnO (yisO), were predicted from the sequence of the Bacillus subtilis genome. We show here that the three genes are expressed differentially during cell growth. In a rich sporulation medium, expression of asnB was detected only during exponential growth, that of asnH was drastically elevated at the transition between exponential growth and stationary phase, and that of asnO was seen only later in sporulation. In a minimal medium, both asnB and asnH were expressed constitutively during exponential growth and in stationary phase, while the expression of asnO was not detected in either phase. However, when the minimal medium was supplemented with asparagine, only the expression of asnH was partially repressed. Transcription analyses revealed that asnB was possibly cotranscribed with a downstream gene, ytnA, while the asnH gene was transcribed as the fourth gene of an operon comprising yxbB, yxbA, yxnB, asnH, and yxaM. The asnO gene is a monocistronic operon, the expression of which was dependent on one of the sporulation sigma factors, sigma-E. Each of the three genes, carried on a low-copy-number plasmid, complemented the asparagine deficiency of an Escherichia coli strain lacking asparagine synthetases, indicating that all encode an asparagine synthetase. In B. subtilis, deletion of asnO or asnH, singly or in combination, had essentially no effect on growth rates in media with or without asparagine. In contrast, deletion of asnB led to a slow-growth phenotype, even in the presence of asparagine. A strain lacking all three genes still grew without asparagine, albeit very slowly, implying that B. subtilis might have yet another asparagine synthetase, not recognized by sequence analysis. The strains lacking asnO failed to sporulate, indicating an involvement of this gene in sporulation.  (+info)

X-ray crystal structure of aminoimidazole ribonucleotide synthetase (PurM), from the Escherichia coli purine biosynthetic pathway at 2.5 A resolution. (2/39)

BACKGROUND: The purine biosynthetic pathway in procaryotes enlists eleven enzymes, six of which use ATP. Enzymes 5 and 6 of this pathway, formylglycinamide ribonucleotide (FGAR) amidotransferase (PurL) and aminoimidazole ribonucleotide (AIR) synthetase (PurM) utilize ATP to activate the oxygen of an amide within their substrate toward nucleophilic attack by a nitrogen. AIR synthetase uses the product of PurL, formylglycinamidine ribonucleotide (FGAM) and ATP to make AIR, ADP and P(i). RESULTS: The structure of a hexahistidine-tagged PurM has been solved by multiwavelength anomalous diffraction phasing techniques using protein containing 28 selenomethionines per asymmetric unit. The final model of PurM consists of two crystallographically independent dimers and four sulfates. The overall R factor at 2.5 A resolution is 19.2%, with an R(free) of 26.4%. The active site, identified in part by conserved residues, is proposed to be a long groove generated by the interaction of two monomers. A search of the sequence databases suggests that the ATP-binding sites between PurM and PurL may be structurally conserved. CONCLUSIONS: The first structure of a new class of ATP-binding enzyme, PurM, has been solved and a model for the active site has been proposed. The structure is unprecedented, with an extensive and unusual sheet-mediated intersubunit interaction defining the active-site grooves. Sequence searches suggest that two successive enzymes in the purine biosynthetic pathway, proposed to use similar chemistries, will have similar ATP-binding domains.  (+info)

A highly conserved mechanism of regulated ribosome stalling mediated by fungal arginine attenuator peptides that appears independent of the charging status of arginyl-tRNAs. (3/39)

The Arg attenuator peptide (AAP) is an evolutionarily conserved peptide involved in Arg-specific negative translational control. It is encoded as an upstream open reading frame (uORF) in fungal mRNAs specifying the small subunit of Arg-specific carbamoyl phosphate synthetase. We examined the functions of the Saccharomyces cerevisiae CPA1 and Neurospora crassa arg-2 AAPs using translation extracts from S. cerevisiae, N. crassa, and wheat germ. Synthetic RNA containing AAP and firefly luciferase (LUC) sequences were used to program translation; analyses of LUC activity indicated that the AAPs conferred Arg-specific negative regulation in each system. The AAPs functioned either as uORFs or fused in-frame at the N terminus of LUC. Mutant AAPs lacking function in vivo did not function in vitro. Therefore, trans-acting factors conferring AAP-mediated regulation are in both fungal and plant systems. Analyses of ribosome stalling in the fungal extracts by primer extension inhibition (toeprint) assays showed that these AAPs acted similarly to stall ribosomes in the region immediately distal to the AAP coding region in response to Arg. The regulatory effect increased as the Arg concentration increased; all of the arginyl-tRNAs examined appeared maximally charged at low Arg concentrations. Therefore, AAP-mediated Arg-specific regulation appeared independent of the charging status of arginyl-tRNA.  (+info)

The yexA gene product is required for phosphoribosylformylglycinamidine synthetase activity in Bacillus subtilis. (4/39)

The yexA gene encodes an 84 amino acid reading frame; in Bacillus subtilis it is positioned between the purC and purQ genes of the purine biosynthetic operon. Disruption of yexA resulted in a purine-auxotrophic phenotype. When yexA was expressed in trans it was able to complement a yexA mutation. Growth experiments and enzyme analysis of yexA mutant strains revealed a defective phosphoribosylformylglycinamidine synthetase (FGAM synthetase). In the organisms in which FGAM synthetase has been studied a single polypeptide is responsible for activity. In some organisms two separate genes - in B. subtilis the purL and purQ genes - encode polypeptides with similarity to the N-terminal and the C-terminal region, respectively, of the single-polypeptide FGAM synthetase. Thus, active FGAM synthetase in B. subtilis requires the yexA gene product in addition to the purL and purQ gene products. Open reading frames with sequence similarity to yexA are found in other Gram-positive organisms, in a cyanobacterium and in methanogenic archaea. The designation purS is proposed for this novel function in purine biosynthesis in B. subtilis.  (+info)

Evolutionarily conserved features of the arginine attenuator peptide provide the necessary requirements for its function in translational regulation. (5/39)

Neurospora crassa arg-2 mRNA contains an evolutionarily conserved upstream open reading frame (uORF) encoding the Arg attenuator peptide (AAP) that confers negative translational regulation in response to Arg. We examined the regulatory role of the AAP and the RNA encoding it using an N. crassa cell-free translation system. AAPs encoded by uORFs in four fungal mRNAs each conferred negative regulation in response to Arg by causing ribosome stalling at the uORF termination codon. Deleting the AAP non-conserved N terminus did not impair regulation, but deletions extending into the conserved region eliminated it. Introducing many silent mutations into a functional AAP coding region did not eliminate regulation, but a single additional nucleotide change altering the conserved AAP sequence abolished regulation. Therefore, the conserved peptide sequence, but not the mRNA sequence, appeared responsible for regulation. AAP extension at its C terminus resulted in Arg-mediated ribosomal stalling during translational elongation within the extended region and during termination. Comparison of Arg-mediated stalling at a rare or common codon revealed more stalling at the rare codon. These data indicate that the highly evolutionarily conserved peptide core functions within the ribosome to cause stalling; translational events at a potential stall site can influence the extent of stalling there.  (+info)

Neonatal pulmonary hypertension--urea-cycle intermediates, nitric oxide production, and carbamoyl-phosphate synthetase function. (6/39)

BACKGROUND: Endogenous production of nitric oxide is vital for the decrease in pulmonary vascular resistance that normally occurs after birth. The precursor of nitric oxide is arginine, a urea-cycle intermediate. We hypothesized that low concentrations of arginine would correlate with the presence of persistent pulmonary hypertension in newborns and that the supply of this precursor would be affected by a functional polymorphism (the substitution of asparagine for threonine at position 1405 [T1405N]) in carbamoyl-phosphate synthetase, which controls the rate-limiting step of the urea cycle. METHODS: Plasma concentrations of amino acids and genotypes of the carbamoyl-phosphate synthetase variants were determined in 65 near-term neonates with respiratory distress. Plasma nitric oxide metabolites were measured in a subgroup of 10 patients. The results in infants with pulmonary hypertension, as assessed by echocardiography, were compared with those in infants without pulmonary hypertension. The frequencies of the carbamoyl-phosphate synthetase genotypes in the study population were assessed for Hardy-Weinberg equilibrium. RESULTS: As compared with infants without pulmonary hypertension, infants with pulmonary hypertension had lower mean (+/-SD) plasma concentrations of arginine (20.2+/-8.8 vs. 39.8+/-17.0 micromol per liter, P<0.001) and nitric oxide metabolites (18.8+/-12.7 vs. 47.2+/-11.2 micromol per liter, P=0.05). As compared with the general population, the infants in the study had a significantly skewed distribution of the genotypes for the carbamoyl-phosphate synthetase variants at position 1405 (P<0.005). None of the infants with pulmonary hypertension were homozygous for the T1405N polymorphism. CONCLUSIONS: Infants with persistent pulmonary hypertension have low plasma concentrations of arginine and nitric oxide metabolites. The simultaneous presence of diminished concentrations of precursors and breakdown products suggests that inadequate production of nitric oxide is involved in the pathogenesis of neonatal pulmonary hypertension. Our preliminary observations suggest that the genetically predetermined capacity of the urea cycle--in particular, the efficiency of carbamoyl-phosphate synthetase--may contribute to the availability of precursors for nitric oxide synthesis.  (+info)

Transfer RNA-dependent amino acid biosynthesis: an essential route to asparagine formation. (7/39)

Biochemical experiments and genomic sequence analysis showed that Deinococcus radiodurans and Thermus thermophilus do not possess asparagine synthetase (encoded by asnA or asnB), the enzyme forming asparagine from aspartate. Instead these organisms derive asparagine from asparaginyl-tRNA, which is made from aspartate in the tRNA-dependent transamidation pathway [Becker, H. D. & Kern, D. (1998) Proc. Natl. Acad. Sci. USA 95, 12832-12837; and Curnow, A. W., Tumbula, D. L., Pelaschier, J. T., Min, B. & Soll, D. (1998) Proc. Natl. Acad. Sci. USA 95, 12838-12843]. A genetic knockout disrupting this pathway deprives D. radiodurans of the ability to synthesize asparagine and confers asparagine auxotrophy. The organism's capacity to make asparagine could be restored by transformation with Escherichia coli asnB. This result demonstrates that in Deinococcus, the only route to asparagine is via asparaginyl-tRNA. Analysis of the completed genomes of many bacteria reveal that, barring the existence of an unknown pathway of asparagine biosynthesis, a wide spectrum of bacteria rely on the tRNA-dependent transamidation pathway as the sole route to asparagine.  (+info)

Carbamoyl-phosphate synthetase. Creation of an escape route for ammonia. (8/39)

Carbamoyl-phosphate synthetase catalyzes the production of carbamoyl phosphate through a reaction mechanism requiring one molecule of bicarbonate, two molecules of MgATP, and one molecule of glutamine. The enzyme from Escherichia coli is composed of two polypeptide chains. The smaller of these belongs to the Class I amidotransferase superfamily and contains all of the necessary amino acid side chains required for the hydrolysis of glutamine to glutamate and ammonia. Two homologous domains from the larger subunit adopt conformations that are characteristic for members of the ATP-grasp superfamily. Each of these ATP-grasp domains contains an active site responsible for binding one molecule of MgATP. High resolution x-ray crystallographic analyses have shown that, remarkably, the three active sites in the E. coli enzyme are connected by a molecular tunnel of approximately 100 A in total length. Here we describe the high resolution x-ray crystallographic structure of the G359F (small subunit) mutant protein of carbamoyl phosphate synthetase. This residue was initially targeted for study because it resides within the interior wall of the molecular tunnel leading from the active site of the small subunit to the first active site of the large subunit. It was anticipated that a mutation to the larger residue would "clog" the ammonia tunnel and impede the delivery of ammonia from its site of production to the site of utilization. In fact, the G359F substitution resulted in a complete change in the conformation of the loop delineated by Glu-355 to Ala-364, thereby providing an "escape" route for the ammonia intermediate directly to the bulk solvent. The substitution also effected the disposition of several key catalytic amino acid side chains in the small subunit active site.  (+info)

*List of MeSH codes (D08)

... carbon-nitrogen ligases with glutamine as amide-n-donor MeSH D08.811.464.259.400.300 --- carbamoyl-phosphate synthase ( ... tyrosine-tRNA ligase MeSH D08.811.464.263.200.950 --- valine-tRNA ligase MeSH D08.811.464.267.500 --- coenzyme a ligases MeSH ... succinate-coa ligases MeSH D08.811.464.754.600 --- dna ligases MeSH D08.811.464.754.720 --- rna ligase (atp) MeSH D08.811. ... isoleucine-trna ligase MeSH D08.811.464.263.200.500 --- leucine-trna ligase MeSH D08.811.464.263.200.550 --- lysine-trna ligase ...
The structures of formylglycinamide ribonucleotide amidotransferase from S. typhimurium either with an unliganded glutaminase domain or in complex with an ATP analogue revealed that no major conformational changes take place upon formation of the glutamyl thioester intermediate or subsequent ATP complexation; therefore, formylglycinamide ribonucleotide binding is proposed to be the mechanism of activation of catalytic coupling in the enzyme ...
cytosol, phosphoribosylamine-glycine ligase activity, phosphoribosylformylglycinamidine cyclo-ligase activity, purine nucleotide biosynthetic process
Numerous significant hits to phosphoribosylformylglycinamidine cyclo-ligase (5-phosphoribosyl-5-aminoimidazole synthetase) proteins in gapped BLAST; e.g. residues 4-343 are 58% similar to gb,AAF95370.1, phosphoribosylformylglycinamidine cyclo-ligase of Vibrio cholerae, residues 3-332 are 59% similar to 10186025 phosphoribosylaminoimidazole synthetase of Escherichia coli, residues 3-332 are 58% similar to 1562541 5-phosphoribosyl-5-aminoimidazole synthetase of Salmonella typhimurium ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
With its passion for pure, natural fibers and exceptional design, Purl Soho is best known for bringing a modern sensibility to traditional crafts. Shop for beautiful yarn, fabric and supplies. Also at purlsoho.com, find the much-loved Purl Bee, our popular resource for free Purl Soho patterns and tons of inspiration!, Collections: Pins + Needles
With its passion for pure, natural fibers and exceptional design, Purl Soho is best known for bringing a modern sensibility to traditional crafts. Shop for beautiful yarn, fabric and supplies. Also at purlsoho.com, find the much-loved Purl Bee, our popular resource for free Purl Soho patterns and tons of inspiration!
Phosphoribosylformylglycinamidine synthaseATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate ...
SWISS-MODEL Repository entry for C3L531 (PURQ_BACAC), Phosphoribosylformylglycinamidine synthase subunit PurQ. Bacillus anthracis (strain CDC 684 / NRRL 3495)
purl bee kitchener stitch. One of the things that specify the sweetness of the purl bee kitchener stitch may be the style of the area. One of the styles that people must attempt may be the Bohemian type.
1CLI: X-ray crystal structure of aminoimidazole ribonucleotide synthetase (PurM), from the Escherichia coli purine biosynthetic pathway at 2.5 A resolution.
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Day 16: Its been one of those days where nothing went right, but Im reminded of this wonderful lady who selflessly loved me and her family with every part of her being. Im thankful for her memory, and for the fact that little me in this pic looks a lot like little Emma ...
That night at dinner, Great Grandma has a mini-stroke (we think). It was scary, and the kids were upset. I corralled them upstairs and we prayed for her. The adults called 911, and thankfully, they were there in minutes. My sister-in-law is a nurse, so she knew what to do. She was only out of it for a few minutes, and then came to and was responsive. But it was awful. Brent rode in the ambulance with her and stayed there for a few hours. All her tests came back normal, and since she is 98, they didnt see a reason to do an EKG. She was back hanging out with us the next day, and we were grateful ...
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The over to the Southwark Playhouse for some theatre. This was good, but Im not very familiar with the Moll Flanders story and Lady Donna assured me they had done their best to remove the vim, vigor and vitality of the women. But the staging was fun and it was an okay evening. A Restoration Greek chorus was kinda cool - can I have one, do we think? Plus Im really glad to have ticked off the Playhouse as I hadnt been before. And for £7 you actually cant go wrong, really. So I shouldnt carp. But, er, I just have. Damn ...
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We have been purchasing grassfed beef from an Amish Farmer and have loved the tenderizing method of pressure cooking. Dried beans can soak quickly and cook to perfection in much less time. Our grandmothers have been using pressure cookers a long time, but the new ones on the market are so much safer. I love that I can cook things that would normally take hours in in under an hour! It is such a time saver ...
knitting) A basic basketweave in which knit and purl stitches alternate in every column ("wale") and every row ("course"), i.e. every knit stitch is flanked on all four sides by purl stitches, and vice versa ...
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I like shawls that are different and so I tried to try a different style for one. BASIC SKILLS NECESSARY You should know how to knit and purl. SIZING / FINISHED MEASUREMENTS 11x41 ...
After my interview with Cecelia Campochiaro about Sequence Knitting, I was very eager to cast on a project and use her innovative techniques. Let me explain briefly what sequence knitting is: by repeating a simple unit of knit and purl stitches over and over again, it is possible to create a complex textured fabric. A simple…
Accepted name: phosphoribosylformylglycinamidine cyclo-ligase. Reaction: ATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. For reaction pathway click here.. Other name(s): phosphoribosylaminoimidazole synthetase; AIR synthetase; 5-aminoimidazole ribonucleotide synthetase. Systematic name: 2-(formamido)-N1-(5-phosphoribosyl)acetamidine cyclo-ligase (ADP-forming). Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9023-53-4. References:. 1. Levenberg, B. and Buchanan, J.M. Properties of the purines. XII. Structure, enzymatic synthesis, and metabolism of 5-aminoimidazole ribotide. J. Biol. Chem. 224 (1957) 1005-1018.. 2. Levenberg, B. and Buchanan, J.M. Properties of the purines. XIII. Structure, enzymatic synthesis, and metabolism of (α-N-formyl)-glycinamidine ribotide. J. Biol. Chem. 224 (1957) 1018-1027.. ...
Rock + Purl Patterns - Lichen Mists Pattern - Detailed Description. Rock + Purl Patterns - Lichen Mists Pattern is available now at Jimmy Beans Wool with Free U.S. Flat Rate shipping for orders over $75, $4 U.S. Flat Rate shipping on all other orders!
1 R. Caliandro, D. Dibenedetto, G. L. Cascarano, A. Mazzone and G. Nico Automatic [alpha]-helix identification in Patterson maps Acta Cryst. D68, 1-12 2 A. Sharma, G. J. Palm, M. Kumari, S. Panjikar, M. V. Jagannadham and W. Hinrichs Purification, crystallization and preliminary crystallographic analysis of banyan peroxidase Acta Cryst. F68, 931-934 3 E. Dall and H. Brandstetter Activation of legumain involves proteolytic and conformational events, resulting in a context- and substrate-dependent activity profile Acta Cryst. F68, 24-31 5 Ruggiero, Alessia; Smaldone, Giovanni; Squeglia, Flavia; Berisio, Rita Enhanced Crystallizability by Protein Engineering Approaches: A General Overview Protein and Peptide Letters V19, 732-742. 6 A. Hoeppner, F. Thomas, A. Rueppel, R. Hensel, W. Blankenfeldt, P. Bayer and A. Faust Structure of the corrinoid:coenzyme M methyltransferase MtaA from Methanosarcina mazei Acta Cryst. D68, 1549-1557. 7 A. S. Tanwar, M. Morar, S. Panjikar and R. Anand Formylglycinamide ...
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This domain is found in carbamoyl dehydratase HypE, which is involved in the maturation of NifE hydrogenase; AIR synthase (PurM) and FGAM synthase (PurL), which are involved in de novo purine biosynthesis; and selenide, water dikinase, an enzyme which synthesizes selenophosphate from selenide and ATP.. In PurM this domain, which has an alpha-beta-type fold, is found at the C terminus of the protein [(PUBMED:10508786)].. ...
Hand knitting is a form of knitting, in which the knitted fabric is produced by hand using needles. Flat knitting uses two straight needles to make generally two-dimensional (flat) pieces. Flat knitting is usually used to knit flat pieces like scarves, blankets, afghans, and the backs, fronts and arms of sweaters (pullovers). In flat knitting, generally stockinette stitch, the hand-knitter knits from right-to-left on one side of the fabric, turns the work (over), and then purls right-to-left back to the starting position. Usually the smooth side of the fabric is considered the right side, the one facing outwards for viewing; and the side that faces inwards, towards the body, the ridged side, is known as the wrong side. Thus, flat knitting involves knitting each row on the right side, then purling each row on the wrong side, etc. If each row is knit (no purls) this creates garter stitch, which has the same appearance on both sides and creates horizontal ridges offset by valleys, rather than a ...
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Id also like to do some knitting today, since Im hoping to get the scarves Im making for my folks done in time for the holidays... but if theyre late, oh well. I had to unravel about a foot of scarf that Id knitted for my moms because I somehow managed to not only add stitches, but to flip my knitting so the knits and purls were off. And I had no idea what row I was on. Oh well. I think the needle gauge that I was using was too big anyways, and my mom is a super petite person, so the scarf at the width it was originally would have been like a short wrap... lolz... so its probably good that I had to rip it out, since now I can use a smaller needle gauge ...
Mode of access: Internet at the NAVY NSGTMO web site. Address as of 9/15/05: http://www.jtfgtmo.southcom.mil/wire.asp; current access is available via PURL ...
This pullover (jumper) is hand knitted of soft black and black purl yarn with stylish collar....Will be a fashionable combination for jeans and tights in all colors....The black one is available and if desired any color can be knit and shipped in 2 weeks ...
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Soft and warm, Seedling Blanket and Lovey are subtly textured fabrics showcasing how simple yet impressive the use of just knit and purl stitches can be. Feel Good Yarn Companys SilverSpun Sport weight yarn brings amazing qualities of warmth, cleanliness and durability allowing your beautiful knitting to stand the test of time, and tiny, loving fingers.
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/ ...
contributors:[{last:Master,middle:Leigh,first:Allison,function:author},{last:Dweck,middle:S ,,first:Carol,function:author},{last:Markman,middle:M ,,first:Ellen,function:author},{last:Walton,middle:M,first:Gregory,function:author},{last:Stanford University. Department of Psychology.,function:author}],title:\I want to try and try\ : increasing achievement motivation in young children,style:apa,source:website,website:{title:\I want to try and try\ : increasing achievement motivation in young children},pubonline:{url:http:\/\/purl.stanford.edu\/hx314zv7838,title:\I want to try and try\ : increasing achievement motivation in young children},oclc:745374760,datasource:http:\/\/www.worldcat.org,pubtype:{main:pubonline ...
But here it is, the almost brainless sock from SWTC:s Tofutsies (727) yarn. I ran into the yarn while I was spring-cleaning my stash and it jumped to the top of the sock-yarn queue. The yarn feels really nice, cottony, and it is nice to knit. I dont care much for the black-red part of the colourway (what looks like a shadow on the photo is not, its the black-red part) for I think the yarn would have been much nicer with just the pink-lilac part. I hope my simple stitch pattern prevents the pooling somewhat but I guess Ill just have to wait and knit. Oh, and this is the first time with square needles too. I cant say I feel a difference compared to normal dpns. Yes, the stitches are even but so are they with normal needles too. Maybe there would be more difference with stockinette at the purl side ...
Regards Klaus - -- Klaus Ethgen http://www.ethgen.ch/ pub 4096R/4E20AF1C 2011-05-16 Klaus Ethgen ,[email protected], Fingerprint: 85D4 CA42 952C 949B 1753 62B3 79D0 B06F 4E20 AF1C -----BEGIN PGP SIGNATURE----- Version: GnuPG v1 iQGcBAEBCgAGBQJUmf0/AAoJEKZ8CrGAGfasR3gMAJeAjfxUYkPXI7VFmnguzTo9 FigWaa7V7xWZahLpHpfjui4jRWFThMKWTpU/fo59XQIw3x7VLHhLg6HWUcjwgRzg TpBocXbb4bhD9xBlPBmr1FGAR/7F4WoExva7AtSMrbRqh31iqjTrrv2gFnLudvsd ZoiA/ocMNMBDI4AFzuaUdCpXl7rrEHB9x6IFNogoRNnDedLY980qts9BCALjmyKM oMtyk0OG+78bFpK4OpEEXk3wHvZHWZADJcYdxa2bIFIdv9n+v40GE8gXc5Q0Dfpn 01GOCVlGUW3jkVvWlWuNtDjFkGDmXGbjfn7dGue3Mco2c8iyT/OWR82/5ZfZBApL 3libnhKyBOjeIErGnnsiIQ4je68MbRCD8+rdtNlpqNy7S12cdQWQUlacAro8H10C tlOciLIjAG/WUwX9gI+9m/asx22BHPsC5RtzLvGArciy1Kzu6CiufgadcoCPFuVK xA64hcpLvxH1uRu/G0NlTtcUrykAipRcL/JeB7JPCA== =jdBh -----END PGP SIGNATURE----- _______________________________________________ pkg-kde-extras mailing list [email protected] http://lists.alioth.debian.org/cgi-bin/mailman/listinfo/pkg-kde-extras ...
Molecular association of cancer cell metastasis with signaling pathways has been explicated so as to aid in the development of new prognostic models for better cancer therapies. However, those metastatic signaling pathways are barely explored to take account of the functions of enzymes involved in cellular metabolism. Particularly, the metabolic enzymes in de novo purine biosynthesis have been overlooked for their potential roles in cancer cell metastasis even though they have been successfully validated anti-cancer drug targets. Meanwhile, several lines of recent discoveries on de novo purine biosynthesis suggest that the spatiotemporal assembly of purine biosynthetic enzymes, the purinosome, is under controls of signaling pathways in cancer cells. The results of the inquiry reveal an unanticipated mechanism of action of 3-phosphoinositide-dependent protein kinase 1 (PDK1) signaling pathways in regulation of purine biosynthesis in an Akt-independent manner. Considering the biological action of ...
Of the various embroidery needles I have, my favorite are the small, overpriced (though perhaps not, since I love the so much) Sajou needles I got from the Purl Soho website last year. At the time, I had just discovered Sajou online and was trying to figure out if there was any way I could swing that lovely set of all of their "Retours Du Nord" embroidery thread (I spent a lot of time on the English version of their website calculating exchange rates and horrified by the cost of shipping to the U.S. from France!). I even tried to think of anyone I knew who might be going to France and asked one friend of my mothers if she would have time on her next business trip to Paris to go out to the Sajou headquarters (she didnt). I finally ordered a selection of individual thread cards from Purl Soho, along with some of the Sajou linen, and the packet of embroidery needles. I love the thread, though I still havent tested its color-fastness and have only used it on a few small cross-stitch samples that ...
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1B6S: Three-dimensional structure of N5-carboxyaminoimidazole ribonucleotide synthetase: a member of the ATP grasp protein superfamily.
Mode of access: Internet from the NTRS web site. Address as of 1/30/07: http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.g ov/20010004249%5F2001002276.pdf; current access is available via PURL ...
Also available via Internet from the GPO Access web site. Addresses as of 8/17/07: http://frwebgate.access.gpo.gov/cgi-bin/getdoc.cgi?dbname=109h̲ ouseh̲earings&docid=f:35566.wais (text version); http://frwebgate.access.gpo.gov/cgi-bin/getdoc.cgi?dbname=109h̲ ouseh̲earings&docid=f:35566.pdf (PDF version) current access is available via PURLs ...
I have Beth Brown-Reisels gansey book which I used to design a gansey to invoke the boyfriend curse (it worked Yay!) The directions in the book were awesome, I really learned a lot of new shaping techniques and was amazed at how clever a lot of the little details were. When I got the sweater back from the ex a little while ago, I seriously contemplated ripping it to make something else out of my handspun - but the diamond shaped arm gussets, the sideways neck gusset. The fake knit/purl seams... I just couldnt do it, and have actually taken to wearing the sweater on a very regular basis! (After I washed the patchouli smell out of it of course ...
The purinosome is a putative multi-enzyme complex that carries out de novo purine biosynthesis within the cell. It is postulated to include all six of the human enzymes identified as direct participants in this ten-step biosynthetic pathway converting phosphoribosyl pyrophosphate to inosine monophosphate: The enzymes of the multi-step de novo purine biosynthesis pathway have been postulated to form a multi-enzyme complex to facilitate substrate channeling between each enzyme of the pathway. Slight variations of the pathway exists between phyla; however, there are 13 enzymes that can be considered part of this biosynthetic pathway. Several individual enzymatic functions have consolidated onto single bifunctional or trifunctional polypeptide chains in higher organisms, suggesting stable physical interactions exist between enzymes. The functional consolidation of steps 2,3, and 5 of the pathway into a single enzyme in higher organisms such as humans suggests physical local proximity of the enzyme ...
prefix dcterms: ,http://purl.org/dc/terms/, . @prefix biro: ,http://purl.org/spar/biro/, . ,https://data.globalchange.gov/reference/233cbcbd-5db5-4bf5-8476-584f88210f2a, dcterms:identifier 233cbcbd-5db5-4bf5-8476-584f88210f2a; ## This reference refers to the following child publication(s): biro:references ,https://data.globalchange.gov/article/10.5194/acp-6-3181-2006,; ## This reference appears in the following GCIS entities: biro:isReferencedBy ,https://data.globalchange.gov/report/climate-science-special-report,; biro:isReferencedBy ,https://data.globalchange.gov/report/climate-science-special-report/chapter/land-cover,; a biro:BibliographicRecord ...
I pulled out about 30 rows in the middle zig-zag column, back down to the misplaced eyelet, and attempted to fix it. I used a long piece of styrofoam and a handful of T pins to keep track of the individual stitches. I turned the sleeve inside out because it is much easier to use a crochet hook to fix knit stitches than purl stitches. And one by one, I started "re-knitting" the row. This is not for the impatient or the faint of heart. As it turns out, it made me sick to my stomach. Literally. After repairing about 15 rows, I was huddled in a ball on the couch, moaning. And feeling chills. And my husband watched me nervously. After a sleepless night, an offer of a visit to the hospital, and a very unproductive day at work, we decided that it probably wasnt the knitting, but rather an unfortunately timed bout of food poisoning. Something similar happened to me many years ago after my first Yoga class. Ive never returned to Yoga.. Tonight, I decided to cling to my Addis and tempt fate with another ...
http://www.purl.org/net/kpml The KPML system offers a robust, mature platform for large-scale grammar engineering that is particularly oriented to multilingual grammar development and generation. It is particularly targetted at providing resources for realistic but broad-coverage generation applications, where both flexibility of expression and speed of generation are at issue-for example in online webpage generation or spoken dialogue. KPML is also used extensively in multilingual text generation research and for teaching. It is based on systemic functional linguistics. The KPML system was a direct descendent of the Penman text generation system, as developed further multilingually in cooperative work between the Komet (http://www.darmstadt.gmd.de/publish/komet/index.html) project in Darmstadt and the Systemic Modelling Group at Macquarie University. Downloadable standalone executables of the system are available for PCs running Windows. The source code is written in ANSI Common Lisp and uses ...
Description: The goal of DDI project is to develop an ontology for the description of drug discovery investigations. DDI aims to follow to the OBO (Open Biomedical Ontologies) Foundry principles, uses relations laid down in the OBO Relation Ontology, and be compliant with Ontology for biomedical investigations (OBI). Institution: Aberystwyth University Contacts: Da Qi, Larisa Soldatova, Ross King, Andrew Hopkins, Richard Bickerton Home Page: http://purl.org/ddi/home ...
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Whether you celebrate Easter or not, theres something about springtime and bunnies that just seems right. A change of weather, a return of farmers markets, Earth Day…all of these things go perfect with a bunny.. Were not the only ones who think so: For the past few weeks weve been noticing all sorts of bunny projects jumping around out there-from teeny tiny ones at Mochimochi Land to finger puppets over at Purl Soho. Now its finally time to share a soft, stuffed friend of our own. So this Free Project Friday, were going with one of our friends from Craft Challenge: Dozens of Ways to Repurpose Scarves: Hoppy!. Download the project here.. And dont forget the template right here.. Or, if youre not up for making anything but really want a stuffed bunny, check out the designers store! Sleepy King on Etsy has all sorts of charming stuffies for sale…. ...
Whether you celebrate Easter or not, theres something about springtime and bunnies that just seems right. A change of weather, a return of farmers markets, Earth Day…all of these things go perfect with a bunny.. Were not the only ones who think so: For the past few weeks weve been noticing all sorts of bunny projects jumping around out there-from teeny tiny ones at Mochimochi Land to finger puppets over at Purl Soho. Now its finally time to share a soft, stuffed friend of our own. So this Free Project Friday, were going with one of our friends from Craft Challenge: Dozens of Ways to Repurpose Scarves: Hoppy!. Download the project here.. And dont forget the template right here.. Or, if youre not up for making anything but really want a stuffed bunny, check out the designers store! Sleepy King on Etsy has all sorts of charming stuffies for sale…. ...
xml record header identifier oai:www.uflib.ufl.edu.ufdc:UF0002830500074datestamp 2008-09-17setSpec [UFDC_OAI_SET]metadata oai_dc:dc xmlns:oai_dc http:www.openarchives.orgOAI2.0oai_dc xmlns:dc http:purl.orgdcelements1.1 xmlns:xsi http:www.w3.org2001XMLSchema-instance xsi:schemaLocation http:www.openarchives.orgOAI2.0oai_dc.xsd dc:title The Jacksonville free press.Mrs. Perrys free pressJacksonville free press.dc:creator Jacksonville free pressdc:subject African Americans -- Newspapers. -- FloridaNewspapers. -- Jacksonville (Fla.)Newspapers. -- Duval County (Fla.)dc:description "Floridas First Coast only quality Black weekly."Also available on microfilm from the University of Florida.Also available on optical disc from Ethnic newswatch.Also available by subscription via the World Wide Web.dc:publisher Rita LuffboroughRita Luffborough Perry,dc:date June 15, 2006dc:type Newspaperdc:format v. : ill. ; 58 cm.dc:identifier http://www.uflib.ufl.edu/ufdc/?b=UF00028305&v=00074002042477 (ALEPH)AKN0341 ...
Wmln 4 s As As. At rrw tss A. A. A. DffUltl www VOLUME XVI NUMBER 46 COLUMBUS, THURSDAY MORNING, SEPTEMBER 2, 1853. JL THE Datlg (Dl)ia State Journal is published every morning, except suxday. St BCOTX BASCOM, JOURNAL BUILDISOS, HIOIl AND PURL ITaZETS ZMIRANCI ON HIGH. tj mall, f 6.00. Dilly during ths ieulon of ths Legislature, and! in- weaKiy lor tno remainder or too year, 66.00. THE WEEKLY JOURNAL Is furnished to city subscribers at R2, und by mall at 01.60 a jroar. Clubs of tour and upwarda, 81.26 vm nu (tin ujnaruii tji,Wi THE TlU-iViSEKLY JOURNAL li 83.00 a year. RATES OF ADVERTISING IN TOT DULY JOURNAL. Tho following rate! and rulea bare bwn agreed upon between the to observed in all own, after this date. July 20, 1849. Ono square, 10 lines or lcaa of thli abed type, for one Insertion. Me. I UU-liiuliMllnn.il Iniurtlnn OR.. " tl ! ! ! ! Ill W CS CO - M CO l8 e I So 8c I Co $o 9 8 S 1 square, 1 263 228 00 4 00 6 00 6. 8. li. , 9 .10. 2 squares, ,1 87 8 87 6 00 0 00 8 00 0. l0. jl2. 10. ...
ID C7QJ90_CATAD Unreviewed; 417 AA. AC C7QJ90; DT 13-OCT-2009, integrated into UniProtKB/TrEMBL. DT 13-OCT-2009, sequence version 1. DT 07-JUN-2017, entry version 57. DE RecName: Full=Phosphoribosylamine--glycine ligase {ECO:0000256,HAMAP-Rule:MF_00138}; DE EC=6.3.4.13 {ECO:0000256,HAMAP-Rule:MF_00138}; DE AltName: Full=GARS {ECO:0000256,HAMAP-Rule:MF_00138}; DE AltName: Full=Glycinamide ribonucleotide synthetase {ECO:0000256,HAMAP-Rule:MF_00138}; DE AltName: Full=Phosphoribosylglycinamide synthetase {ECO:0000256,HAMAP-Rule:MF_00138}; GN Name=purD {ECO:0000256,HAMAP-Rule:MF_00138}; GN OrderedLocusNames=Caci_0279 {ECO:0000313,EMBL:ACU69232.1}; OS Catenulispora acidiphila (strain DSM 44928 / NRRL B-24433 / NBRC OS 102108 / JCM 14897). OC Bacteria; Actinobacteria; Catenulisporales; Catenulisporaceae; OC Catenulispora. OX NCBI_TaxID=479433 {ECO:0000313,EMBL:ACU69232.1, ECO:0000313,Proteomes:UP000000851}; RN [1] {ECO:0000313,EMBL:ACU69232.1, ECO:0000313,Proteomes:UP000000851} RP NUCLEOTIDE SEQUENCE ...
Anmerkung: Aufgrund der häufig nicht stabilen Adressen von Dokumenten auf dem World Wide Web haben die Autoren versucht, solche Zitate auf gedruckte Werke zurückzuführen. Wo dies nicht möglich war, empfiehlt sich eine Suche des Titels mittels einer Suchmaschine, sollte die URL nicht mehr aktuell sein.. Anonym (1990): Understanding our genetic inheritance the U.S. human genome project: the first five years. U.S. Dept of Health and Human Services, U.S. Dept of Energy. [http://www.nhgri.nih.gov/HGP/HGP_goals/5yrplan.html]. Anonym (1991): Übereinkommen zum Schutz der Alpen (Alpenkonvention). Salzburg 1991 [http://deutsch.cipra.org/texte/alpenkonvention/alpenkonven-tion_hauptseite.htm]. Anonym (1996): Dublin Core Metadata Element Set: Reference Description. OCLC Online Computer Library Center, Inc. [http://purl.org/metadata/dublin _core_elements]. Anonym (1999): Final report of the OECD Megascience Forum Working Group on Biological Informatics, January, 1999. ...
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InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
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Get to know all of Khatu Shyam Institute of Management & Technology, Janak Puri. Find information like reviews, ratings & fee structure of Janak Puri, Khatu Shyam Institute of Management & Technology.
TY - JOUR. T1 - A stromal organiser cell that directs neonatal spleen regeneration. AU - Tan, Jon. AU - Watanabe, Takeshi. PY - 2015/9. Y1 - 2015/9. UR - http://purl.org/au-research/grants/NHMRC/1078247. U2 - 10.1016/j.exphem.2015.06.265. DO - 10.1016/j.exphem.2015.06.265. M3 - Meeting Abstract. VL - 43. SP - S97-S97. JO - Experimental Hematology. JF - Experimental Hematology. SN - 0301-472X. IS - 9. ER - ...
Вчера приползли в тренировки, на автопилоте выглушили по post workout shake, и тут я осознала, что мысль о готовке вызывает отвращение. По плану были куриные грудки, фаршированные фигами и клювкой, но я забыла купить нужные свежие травки, и вообще, возиться мне совершенно не хотелось. В итоге решила отступить от плана и попробовать совем другой рецепт - Clean chicken picatta with chickpea puree.. В 4 руки с мужем быстренько все приготовили, а потом сами от себя тащились. Получилось необыкновенно вкусно, а учитывая простоту процесса, вообще находка! Муж, любитель картофельного ...
Tingkat kejadian kanker payudara menempati tumor ganas di seluruh tubuh 7%-10%. Wanita dalam masa hidupnya kemungkinan mengidap kanker payudara mencapai 10%
Menurutnya, perusahaan penjaminan kredit berperan sebagai pihak pemberi garansi kredit, sehingga modal ventura yang disalurkan ke startup terjaga keamaanannya dan jika terjadi risiko penjaminan langsung turun langsung untuk mencovernya.
Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators Cell envelope-associated transcriptional attenuator LytR-CpsA-Psr, subfamily F2 ...
Phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase (PAICS), an enzyme required for de novo purine biosynthesis, is associated with and involved in tumorigenesis. This study aimed to evaluate the role of PAICS in human breast cancer, which remains the most frequently diagnosed cancer and the leading cause of cancer-related death among women in less developed countries. Lentivirus-based short hairpin RNA targeting PAICS specifically depleted its endogenous expression in ZR-75-30 and MDA-MB-231 breast cancer cells. Depletion of PAICS led to a significant decrease in cell viability and proliferation. To ascertain the mechanisms through which PAICS modulates cell proliferation, flow cytometry was performed, and it was confirmed that G1-S transition was blocked in ZR-75-30 cells through PAICS knockdown. This might have occurred partly through the suppression of Cyclin E and the upregulation of Cyclin D1, P21, and CDK4. Moreover, PAICS knockdown obviously
Define carbocyclic. carbocyclic synonyms, carbocyclic pronunciation, carbocyclic translation, English dictionary definition of carbocyclic. adj. Having a ring composed exclusively of carbon atoms, as benzene. adj containing a closed ring of carbon atoms Adj. 1. carbocyclic - having or relating...
Adenylosuccinate lyase is an enzyme of fumarase superfamily that participates in the purine biosynthetic pathway, catalysing the nonhydrolytic cleavage of succinyl groups from SAICA ribotide and adenylosuccinate. Enzyme defects are associated with a
Hindustan TimesVeteran Bollywood actor Om Puri died of a heart attack Friday morning. He was 66. Om Puri was a versatile actor who excelled in parallel cinema as well as he pioneered in commercial cinema. An actor who broke stereotypes and left a significant mark in Indian cinema.
uc berkeley In five years, Ashwin Puri has filled the University of California, Berkeleys sports marketing coffers to among the highest levels in college athletics. Puri, Cals senior associate athletic director and chief revenue officer, was principally involved in the Pac-12 school&r
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Exhibits hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides. Involved in several processes, including cellular amino acid metabolic process; energy homeostasis; and regulation of oxidative phosphorylation uncoupler activity. Localizes to the extracellular space. Orthologous to human PM20D1 (peptidase M20 domain containing 1 ...
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Bollywood Best Actor /Comedian Om puri Apologises for his Comment on Uri Martyrs. On the issue of Indian Soldiers ( Jawans ) killed during the Uri attacks of 18 Sept. In a TV Channel ,He said, "Did anybody force them (the soldiers) to join the army?". Due to this A FIR under sedition Case filed against the actor under Section 124[A] Under Indian Penal Code Due to his Commenting On Indian Martyrs .. ...
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PS You can check the following websites for relevant reports, all of which I was the principal author and/or investigator on (also listed some presentations available on the web). SOME REPORTS BY THE AUTHOR AVAILABLE ON THE WEB Benemann, J.R. ; Goebel, R.P. ; Weissman, J.C. ; Augenstein, D.C. 1982. "Microalgae as a source of liquid fuels". Final technical report USDOE -OER http://www.osti.gov/bridge/product.biblio.jsp?query_id=0&page=0&osti_id=6374113. Benemann J.R. and W.J. Oswald. Final Report to US DOE NETL, 1996 "Systems and Economic analysis of microalgae ponds for conversion of CO2 to biomass" At: http://www.osti.gov/bridge/servlets/purl/493389-FXQyZ2/webviewable/493389.pdf. Benemann, J.R., 2002 "A Technology Roadmap for Greenhouse Gas Abatement with Microalgae". Report to the U.S. Department of Energy, National Energy Technology Laboratory, and the International Energy Agency Greenhouse Gas Abatement Programme, http://www.co2captureandstorage.info/networks/Biofixation.htm. Sheehan, J., ...
E coli purU protein: catalyzes the conversion of 10-formyltetrahydrofolate to tetrahydrofolate & formate; amino acid sequence has been determined; part of the tyrT system in E coli; has significant homology to the PurN protein of E coli & to enzymes with glycinamide ribonucleotide transformylase activity; GenBank M64675 (e coli)
CAD antibody (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) for ICC/IF, WB. Anti-CAD pAb (GTX28406) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
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Comprehensive nutrition resource for Prune Puree. Learn about the number of calories and nutritional and diet information for Prune Puree. This is part of our comprehensive database of 40,000 foods including foods from hundreds of popular restaurants and thousands of brands.

List of MeSH codes (D08) - WikipediaList of MeSH codes (D08) - Wikipedia

... carbon-nitrogen ligases with glutamine as amide-n-donor MeSH D08.811.464.259.400.300 --- carbamoyl-phosphate synthase ( ... tyrosine-tRNA ligase MeSH D08.811.464.263.200.950 --- valine-tRNA ligase MeSH D08.811.464.267.500 --- coenzyme a ligases MeSH ... succinate-coa ligases MeSH D08.811.464.754.600 --- dna ligases MeSH D08.811.464.754.720 --- rna ligase (atp) MeSH D08.811. ... isoleucine-trna ligase MeSH D08.811.464.263.200.500 --- leucine-trna ligase MeSH D08.811.464.263.200.550 --- lysine-trna ligase ...
more infohttps://en.wikipedia.org/wiki/List_of_MeSH_codes_(D08)

faah2 Protein, fatty acid amide hydrolase 2 - Creative BioMartfaah2 Protein, fatty acid amide hydrolase 2 - Creative BioMart

... including those from the three main classes of fatty acid amides; N-acylethanolamines, fatty acid primary amides and N-acyl ... This gene encodes a fatty acid amide hydrolase that shares a conserved protein motif with the amidase signature family of ... Function carbon-nitrogen ligase activity, with glutamine as amido-N-donor. Related Protein GATB; FAAH2B; PET112L; FAAH2; FAAH2A ... FAAH2 has several biochemical functions, for example, carbon-nitrogen ligase activity, with glutamine as amido-N-donor, ...
more infohttps://www.creativebiomart.net/symbolsearch_FAAH2.htm

Human Metabolome Database: Showing metabocard for Ethanolamine (HMDB0000149)Human Metabolome Database: Showing metabocard for Ethanolamine (HMDB0000149)

Fatty-acid amide hydrolase 1. General function:. Involved in carbon-nitrogen ligase activity, with glutamine as amido-N-donor. ... Fatty-acid amide hydrolase 2. General function:. Involved in carbon-nitrogen ligase activity, with glutamine as amido-N-donor. ... Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their ... Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their ...
more infohttp://www.hmdb.ca/metabolites/HMDB00149

FAAH2 Gene - GeneCards | FAAH2 Protein | FAAH2 AntibodyFAAH2 Gene - GeneCards | FAAH2 Protein | FAAH2 Antibody

Fatty Acid Amide Hydrolase 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The ... GO annotations related to this gene include hydrolase activity and carbon-nitrogen ligase activity, with glutamine as amido-N- ... Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their ... CRISPR: plasmid gRNA, lentiviral gRNA, and donor plasmid. *Bacterial expression: pET, pBAD, and pCS ...
more infohttp://www.genecards.org/cgi-bin/carddisp.pl?gene=FAAH2

A transgenerational role of the germline nuclear RNAi pathway in repressing heat stress-induced transcriptional activation in C...A transgenerational role of the germline nuclear RNAi pathway in repressing heat stress-induced transcriptional activation in C...

... fatty acid amide hydrolase 2); faah-5 is predicted to have carbon-nitrogen ligase activity, with glutamine as amido-N-donor, ... Young adults were pulverized by grinding in liquid nitrogen with a mortar and pestle immediately after sample harvest and were ... faah-5 is an ortholog of human FAAH (fatty acid amide hydrolase) and FAAH2 ( ...
more infohttps://epigeneticsandchromatin.biomedcentral.com/articles/10.1186/s13072-016-0052-x

Effect of Genetically Low 25-Hydroxyvitamin D on Mortality Risk: Mendelian Randomization Analysis in 3 Large European Cohorts. ...Effect of Genetically Low 25-Hydroxyvitamin D on Mortality Risk: Mendelian Randomization Analysis in 3 Large European Cohorts. ...

Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor/genetics. *Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor/ ... Oxidoreductases Acting on CH-CH Group Donors. *7-dehydrocholesterol reductase. *Carbon-Nitrogen Ligases with Glutamine as Amide ...
more infohttps://phgkb.cdc.gov/PHGKB/phgHome.action?action=forward&dbsource=phg&id=22907

Effect of Genetically Low 25-Hydroxyvitamin D on Mortality Risk: Mendelian Randomization Analysis in 3 Large European Cohorts. ...Effect of Genetically Low 25-Hydroxyvitamin D on Mortality Risk: Mendelian Randomization Analysis in 3 Large European Cohorts. ...

Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor/genetics. *Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor/ ... Oxidoreductases Acting on CH-CH Group Donors. *7-dehydrocholesterol reductase. *Carbon-Nitrogen Ligases with Glutamine as Amide ...
more infohttps://phgkb.cdc.gov/PHGKB/phgHome.action?action=forward&dbsource=cluster&id=23457

Search Articles | University of Toronto LibrariesSearch Articles | University of Toronto Libraries

Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor - genetics , Gene mutations , Dairy cattle , Genetic research , Genetic ... DNA-LIGASE-IV , IMMUNODEFICIENCY , Protein Kinases - metabolism , Synthetic Lethal Mutations - genetics , Protein Kinases - ...
more infohttps://query.library.utoronto.ca/index.php/search/q?kw=SubjectTerms:EMBRYONIC%20LETHALITY

Search Articles | University of Toronto LibrariesSearch Articles | University of Toronto Libraries

Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor - antagonists & inhibitors , Carbon-Nitrogen Ligases with Glutamine as ... Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor - genetics , MEDICINE, RESEARCH & EXPERIMENTAL , BIOCHEMISTRY & ... Amide-N-Donor - metabolism , Liver Neoplasms - genetics , Humans , Stomach Neoplasms - therapy , Metabolome , Stomach Neoplasms ...
more infohttps://query.library.utoronto.ca/index.php/search/q?kw=Author:Gregory%20V.%20Kryukov

KEGG BRITE: Enzymes - Bacillus anthracis CDC 684KEGG BRITE: Enzymes - Bacillus anthracis CDC 684

6.3.4 Other carbon-nitrogen ligases 6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor ... 6.3 Forming carbon-nitrogen bonds 6.3.1 Acid-D-ammonia (or amine) ligases (amide synthases) ... 6.3.5.9 hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) 6.3.5.10 adenosylcobyric acid synthase (glutamine- ... 6.3.5.7 glutaminyl-tRNA synthase (glutamine-hydrolysing) BAMEG_0380 gatA; glutamyl-tRNA(Gln) amidotransferase, A subunit BAMEG_ ...
more infohttps://www.genome.jp/kegg-bin/get_htext?bah01000+BAMEG_0379

Bra008148 protein (Brassica rapa) - STRING interaction networkBra008148 protein (Brassica rapa) - STRING interaction network

... carbon-nitrogen ligase, with glutamine as amido-N-donor / glutaminyl-tRNA synthase (glutamine-hydrolyzing)/ ligase (108 aa) ... ligase AT5G64440 (E=7e-285) AtFAAH , AtFAAH (Arabidopsis thaliana fatty acid amide hydrolase); N-(long-chain-acyl)ethanolamine ... carbon-nitrogen ligase, with glutamine as amido-N-donor / glutaminyl-tRNA synthase (glutamine-hydrolyzing)/ ligase (531 aa) ... carbon-nitrogen ligase, with glutamine as amido-N-donor / glutaminyl-tRNA synthase (glutamine-hydrolyzing)/ ligase ...
more infohttps://string-db.org/network/3711.Bra008148.1-P

ENZYME search by enzyme classENZYME search by enzyme class

Cyclo-ligases. 6. 3. 4.- Other carbon--nitrogen ligases. 6. 3. 5.- Carbon--nitrogen ligases with glutamine as amido-N-donor. 6 ... 6. 2. 1.- Acid--thiol ligases. 6. 3. -.- Forming carbon-nitrogen bonds. 6. 3. 1.- Acid--ammonia (or amine) ligases (amide ... 4. 3.99.- Other carbon-nitrogen lyases. 4. 4. -.- Carbon-sulfur lyases. 4. 4. 1.- Carbon-sulfur lyases. 4. 5. -.- Carbon-halide ... 3. 5. -.- Acting on carbon-nitrogen bonds, other than peptide bonds. 3. 5. 1.- In linear amides. 3. 5. 2.- In cyclic amides. 3 ...
more infohttps://enzyme.expasy.org/enzyme-byclass.html

Recombinant Human FAAH2 293 Cell Lysate FAAH2-6480HCL - Creative BioMartRecombinant Human FAAH2 293 Cell Lysate FAAH2-6480HCL - Creative BioMart

carbon-nitrogen ligase activity, with glutamine as amido-N-donor; hydrolase activity;. ... FAAH2; fatty acid amide hydrolase 2; AMDD, amidase domain containing; fatty-acid amide hydrolase 2; FAAH 2; FLJ31204; RP11 ... Antigen standard for fatty acid amide hydrolase 2 (FAAH2) is a lysate prepared from HEK293T cells transiently transfected with ...
more infohttps://www.creativebiomart.net/description_408145_318.htm

Principles of Biochemistry/Nucleic acid III: Sythesis of nucleotides - Wikibooks, open books for an open worldPrinciples of Biochemistry/Nucleic acid III: Sythesis of nucleotides - Wikibooks, open books for an open world

... specifically those forming carbon-nitrogen bonds carbon-nitrogen ligases with glutamine as amido-N-donor. The systematic name ... A novel method is by reaction of certain amides with carbonitriles under electrophilic activation of the amide with 2-chloro- ... L-glutamine amido-ligase (AMP-forming). Other names in common use include GMP synthetase (glutamine-hydrolysing), guanylate ... The first committed step is the reaction of PRPP, glutamine and water to 5-phosphoribosylamine, glutamine, and pyrophosphate ...
more infohttps://en.wikibooks.org/wiki/Principles_of_Biochemistry/Nucleic_acid_III:_Sythesis_of_nucleotides

Methylglyoxal synthase-like superfamilyMethylglyoxal synthase-like superfamily

... carbon-nitrogen ligase activity, with glutamine as amido-N-donor ,, hydrolase activity, acting on carbon-nitrogen (but not ... acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides ,, hydrolase activity, acting on carbon-nitrogen (but not ... nitrogen ligases with glutamine as amido-N-donor. 0. Informative. Direct. Enzyme Commission (EC). Other carbon--nitrogen ... Carbon--nitrogen ligases with glutamine as amido-N-donor. 0. 1.728. Informative. DIRECT. ...
more infohttp://supfam.cs.bris.ac.uk/SUPERFAMILY/cgi-bin/scop.cgi?ipid=SSF52335

Chymostatin - DrugBankChymostatin - DrugBank

Carbon-nitrogen ligase activity, with glutamine as amido-n-donor. Specific Function. Not Available. Gene Name. pam. Uniprot ID ... Alpha amino acid amides / Phenylpropanoic acids / Beta amino acids and derivatives / Amphetamines and derivatives / N-acyl ... Alpha-amino acid amide / 3-phenylpropanoic-acid / Beta amino acid or derivatives / N-substituted-alpha-amino acidAmphetamine or ... Organic nitrogen compound / Carbonyl group / Aldehyde / Organic oxygen compound / Organopnictogen compound / Organic oxide / ...
more infohttps://www.drugbank.ca/drugs/DB01683

Metabolism, Part 2 - Exam #1 Flashcards by whitney miller | BrainscapeMetabolism, Part 2 - Exam #1 Flashcards by whitney miller | Brainscape

N-glycosylation - attached at the Amide Nitrogen of the polypeptide side chain -O-glycosylation - attached at the Hydroxyl ... CANNOT be synthesized from precursor carbon-skeletons in the body and must be consumed preformed in the diet;. -Carbon ... Contains glutamine synthetase (make) an glutaminase (break) in different areas;. -Liver is not a net producer or consumer of ... Ligases - (syntheses) join compounds. -Oxidoreductases - transfer electrons. -Transferases - move functional groups 4 ...
more infohttps://www.brainscape.com/flashcards/metabolism-part-2-exam-1-1929171/packs/3337284

US Patent # 4,299,916. Preferential signal production on a surface in immunoassays - Patents.comUS Patent # 4,299,916. Preferential signal production on a surface in immunoassays - Patents.com

6.3.3 Cylo-ligases 6.3.4 Other C--N ligases 6.3.5 C--N ligases with glutamine as N-donor 6.4 Forming C--C bonds ... carbon-oxygen lyases 4.3 Carbon-nitrogen lyases 4.3.1 Ammonia-lyases 4.3.2 Amidine-lyases 4.4 Carbon-sulfur lyases 4.5 Carbon- ... S bonds 6.2.1 Acid-thiol ligases 6.3 Forming C--N bonds 6.3.1 Acid-ammonia ligases (amide synthetases) 6.3.2 Acid-aminoacid ... preferably bonded solely to carbon, while nitrogen will normally be present as amino, bonded solely to carbon, or amido, while ...
more infohttp://patents.com/us-4299916.html

Redox Modulations, Antioxidants, and Neuropsychiatric DisordersRedox Modulations, Antioxidants, and Neuropsychiatric Disorders

... glutamine and cysteine are combined into γ-glutamylcysteine where a further addition of glutamine produces glutathione. ... 5.3.1. N-Acetylcysteine (NAC) and N-Acetylcysteine Amide (NACA). Once NAC or NACA reaches the cells, it is absorbed into the ... B. Poeggeler, S. Saarela, R. J. Reiter et al., "Melatonin-a highly potent endogenous radical scavenger and electron donor: new ... ROS, reactive oxygen species; RNS, reactive nitrogen species; Nrf2, nuclear factor- (erythroid-derived-2-) like 2; Keap1, Kelch ...
more infohttps://www.hindawi.com/journals/omcl/2016/4729192/

Patents for Heterogeneous or solid phase assay system - PatentorgPatents for Heterogeneous or solid phase assay system - Patentorg

Acting on Carbon to Nitrogen Bond Other Than Peptide Bond (3.5) 8 ... Acting on Nitrogen-Containing Compound as Donor (1.2, 1.5, 1.7) ... Amide. *Preparing sulfur-containing organic compound. *Preparing organic compound containing a metal or atom other than H, N, C ... Glutamic acid; glutamine. *Methionine; cysteine; cystine. *Citrulline; arginine; ornithine. *Lysine; diaminopimelic acid; ... Involving a ligase (6.). *Involving a hydrolase (3.). *Mashing or wort making ...
more infohttp://www.patentorg.com/435-7.92-heterogeneous-or-solid-phase-assay

View source for FF:11684-122I1 - resource browserView source for FF:11684-122I1 - resource browser

... acting on carbon-nitrogen (but not peptide) bonds, in linear amides;0.0132230642488399!GO:0016481;negative regulation of ... ligase activity, forming carbon-nitrogen bonds;2.11525603869065e-07!GO:0005319;lipid transporter activity;2.19020038963518e-07! ... acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and ... glutamine family amino acid metabolic process;0.000243438906009876!GO:0009117;nucleotide metabolic process;0.000250016609167851 ...
more infohttps://fantom.gsc.riken.jp/5/sstar/index.php?title=FF:11684-122I1&action=edit&oldid=3281795

BIOL0280 Third Midterm Examination 2014 - Foodelphi.comBIOL0280 Third Midterm Examination 2014 - Foodelphi.com

C) It uses NH4+ as a nitrogen source D) It uses glutamine as a nitrogen source Circle the correct answer 8. [2 points] Which of ... B) Carbon atoms of tetrapyrroles are derived from glycine and succinyl-CoA C) Carbon atoms of tetrapyrroles are derived from ... an amide bond is formed between puromycin and peptide_ 23. [4 points] Give two examples of coactivators and explain how they ... The arrangement of hydrogen bond acceptors and donors differs in AT and TA base pairs, allowing proteins to differentiate the ...
more infohttps://www.foodelphi.com/biol0280-third-midterm-examination-2014/

Carbamoyl phosphate synthetase, small subunit N-terminal domain superfamilyCarbamoyl phosphate synthetase, small subunit N-terminal domain superfamily

... nitrogen ligases with glutamine as amido-N-donor. 0. Informative. Direct. Enzyme Commission (EC). Other carbon--nitrogen ... Carbon--nitrogen ligases with glutamine as amido-N. 0. Informative. Direct. Enzyme Commission (EC). In cyclic amides. 0.0002974 ... Carbon--nitrogen ligases with glutamine as amido-N-donor. 0. 1.675. Informative. DIRECT. ... Forming carbon-nitrogen bonds. 0. 0.9067. Moderately Informative. DIRECT. Enzyme Commission (EC). Carbon--nitrogen ligases with ...
more infohttp://supfam.org/SUPERFAMILY/cgi-bin/scop.cgi?ipid=SSF52021

Carbamoyl phosphate synthetase, large subunit connection domain superfamilyCarbamoyl phosphate synthetase, large subunit connection domain superfamily

... nitrogen ligases with glutamine as amido-N-donor. 0. Informative. Direct. Enzyme Commission (EC). Other carbon--nitrogen ... Carbon--nitrogen ligases with glutamine as amido-N. 0. Informative. Direct. Enzyme Commission (EC). In cyclic amides. 0.00091. ... Carbon--nitrogen ligases with glutamine as amido-N-donor. 0. 1.651. Informative. DIRECT. ... Forming carbon-nitrogen bonds. 0. 0.9067. Moderately Informative. DIRECT. Enzyme Commission (EC). Carbon--nitrogen ligases with ...
more infohttp://supfam.org/SUPERFAMILY/cgi-bin/scop.cgi?sunid=48108
  • Thus, glutamine deamidation, performed by numerous enzymes in the cells, yields glutamate, which can further be transformed into proline through a series of reductive steps, as well as into aspartate and asparagine, via the utilization of oxidative reactions of the tricarboxylic acid (TCA) cycle. (cloudfront.net)
  • A) Aspartate B) Carbamoyl phosphate C) Glutamine D) Histidine E) Tryptophan F) Lysine G) Glycine H) Ornithine Circle all correct answer(s) More than one answer may be correct.2 points for all correct, 0.5 point off for any incorrect or 0.5 point off for any missing answer. (foodelphi.com)
  • The complex metabolic logic of the proliferating cancer cells' appetite for glutamine-which goes far beyond satisfying their protein synthesis requirements-has only recently come into focus. (cloudfront.net)
  • However, consumption of glutamine in proliferating cells far exceeds the demands imposed by protein synthesis. (cloudfront.net)
  • Despite the fact that glutamine is a nonessential amino acid that can be synthesized by cells from glucose‐derived carbons and amino acid‐derived ammonia, most mammalian cells in tissue culture cannot proliferate or even survive in an environment that does not contain millimolar levels of glutamine. (cloudfront.net)
  • Plants have also developed systems for ammonium reassimilation from secondary sources to avoid losing biological nitrogen. (biomedcentral.com)
  • Biochemistry textbooks and cell culture experiments seem to be telling us two different things about the significance of external glutamine supply for mammalian cell growth and proliferation. (cloudfront.net)
  • In this review, we examine the diversity of biosynthetic and regulatory uses of glutamine and their role in proliferation, stress resistance, and cellular identity, as well as discuss the mechanisms that cells utilize in order to adapt to glutamine limitation. (cloudfront.net)
  • L-glutamine appears to be required to support the proliferation of mitogen-stimulated lymphocytes, as well as the production of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma). (drugbank.ca)
  • The demand for L-glutamine by the intestine, as well as by cells such as lymphocytes, appears to be much greater than that supplied by skeletal muscle, the major storage tissue for L-glutamine. (drugbank.ca)
  • The first committed step is the reaction of PRPP, glutamine and water to 5'-phosphoribosylamine, glutamine, and pyrophosphate - catalyzed by pyrophosphate amidotransferase, which is activated by PRPP and inhibited by AMP, GMP and IMP. (wikibooks.org)
  • It is the principal carrier of nitrogen in the body and is an important energy source for many cells. (drugbank.ca)
  • L-glutamine helps maintain secretory IgA, which functions primarily by preventing the attachment of bacteria to mucosal cells. (drugbank.ca)
  • L-glutamine appears to play a major role in protecting the integrity of the gastrointestinal tract and, in particular, the large intestine. (drugbank.ca)
  • The consequence of the high demand of proliferating cells for glutamine is the disproportionate depletion of the latter from the surrounding environment. (cloudfront.net)
  • Therefore, supplying supplemental L-glutamine under these conditions may do a number of things. (drugbank.ca)