A class of oxidized LDL receptors that contain LECTIN-like extracellular domains.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A class of animal lectins that bind specifically to beta-galactoside in a calcium-independent manner. Members of this class are distiguished from other lectins by the presence of a conserved carbohydrate recognition domain. The majority of proteins in this class bind to sugar molecules in a sulfhydryl-dependent manner and are often referred to as S-type lectins, however this property is not required for membership in this class.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
An abundant pulmonary surfactant-associated protein that binds to a variety of lung pathogens and enhances their opsinization and killing by phagocytic cells. Surfactant protein D contains a N-terminal collagen-like domain and a C-terminal lectin domain that are characteristic of members of the collectin family of proteins.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
Carbohydrates present in food comprising digestible sugars and starches and indigestible cellulose and other dietary fibers. The former are the major source of energy. The sugars are in beet and cane sugar, fruits, honey, sweet corn, corn syrup, milk and milk products, etc.; the starches are in cereal grains, legumes (FABACEAE), tubers, etc. (From Claudio & Lagua, Nutrition and Diet Therapy Dictionary, 3d ed, p32, p277)
The study of those aspects of energy and matter in terms of elementary principles and laws. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The remnants of plant cell walls that are resistant to digestion by the alimentary enzymes of man. It comprises various polysaccharides and lignins.
A numerical system of measuring the rate of BLOOD GLUCOSE generation from a particular food item as compared to a reference item, such as glucose = 100. Foods with higher glycemic index numbers create greater blood sugar swings.
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
Inborn errors of metabolism characterized by defects in specific lysosomal hydrolases and resulting in intracellular accumulation of unmetabolized substrates.
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.
A group of severe neurodegenerative diseases characterized by intracellular accumulation of autofluorescent wax-like lipid materials (CEROID; LIPOFUSCIN) in neurons. There are several subtypes based on mutations of the various genes, time of disease onset, and severity of the neurological defects such as progressive DEMENTIA; SEIZURES; and visual failure.
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
A group of enzymatic disorders affecting the nervous system and to a variable degree the skeletal system, lymphoreticular system, and other organs. The conditions are marked by an abnormal accumulation of catabolic material within lysosomes.
The bacterial sugar phosphotransferase system (PTS) that catalyzes the transfer of the phosphoryl group from phosphoenolpyruvate to its sugar substrates (the PTS sugars) concomitant with the translocation of these sugars across the bacterial membrane. The phosphorylation of a given sugar requires four proteins, two general proteins, Enzyme I and HPr and a pair of sugar-specific proteins designated as the Enzyme II complex. The PTS has also been implicated in the induction of synthesis of some catabolic enzyme systems required for the utilization of sugars that are not substrates of the PTS as well as the regulation of the activity of ADENYLYL CYCLASES. EC 2.7.1.-.
A species of gram-positive, rod-shaped bacteria isolated from the intestinal tract of humans and animals, the human mouth, and vagina. This organism produces the fermented product, acidophilus milk.
A group of enzymes that catalyzes the transfer of a phosphate group onto a nitrogenous group acceptor. EC 2.7.3.
A genus of gram-positive, microaerophilic, rod-shaped bacteria occurring widely in nature. Its species are also part of the many normal flora of the mouth, intestinal tract, and vagina of many mammals, including humans. Pathogenicity from this genus is rare.
A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.
Protective measures against unauthorized access to or interference with computer operating systems, telecommunications, or data structures, especially the modification, deletion, destruction, or release of data in computers. It includes methods of forestalling interference by computer viruses or so-called computer hackers aiming to compromise stored data.
The privacy of information and its protection against unauthorized disclosure.
The state of being free from intrusion or disturbance in one's private life or affairs. (Random House Unabridged Dictionary, 2d ed, 1993)
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
A short thick vein formed by union of the superior mesenteric vein and the splenic vein.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
Sequential operating programs and data which instruct the functioning of a digital computer.
A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.

Variable domain-linked oligosaccharides of a human monoclonal IgG: structure and influence on antigen binding. (1/5581)

The variable-domain-attached oligosaccharide side chains of a human IgG produced by a human-human-mouse heterohybridoma were analysed. In addition to the conserved N-glycosylation site at Asn-297, an N-glycosylation consensus sequence (Asn-Asn-Ser) is located at position 75 in the variable region of its heavy chain. The antibody was cleaved into its antigen-binding (Fab) and crystallizing fragments. The oligosaccharides of the Fab fragment were released by digestion with various endo- and exoglycosidases and analysed by anion-exchange chromatography and fluorophore-assisted carbohydrate electrophoresis. The predominant components were disialyl- bi-antennary and tetra-sialyl tetra-antennary complex carbohydrates. Of note is the presence in this human IgG of oligosaccharides containing N-glycolylneuraminic acid and N-acetylneuraminic acid in the ratio of 94:6. Furthermore, we determined N-acetylgalactosamine in the Fab fragment of this antibody, suggesting the presence of O-linked carbohydrates. A three-dimensional structure of the glycosylated variable (Fv) fragment was suggested using computer-assisted modelling. In addition, the influence of the Fv-associated oligosaccharides of the CBGA1 antibody on antigen binding was tested in several ELISA systems. Deglycosylation resulted in a decreased antigen-binding activity.  (+info)

Structural characterization of the N-linked oligosaccharides in bile salt-stimulated lipase originated from human breast milk. (2/5581)

The detailed structures of N- glycans derived from bile salt-stimulated lipase (BSSL) found in human milk were determined by combining exoglycosidase digestion with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The N- glycan structures were conclusively determined in terms of complexity and degree of fucosylation. Ion-exchange chromatography with pulsed amperometric detection, together with mass-spectral analysis of the esterified N- glycans, indicated the presence of monosialylated structures. The molecular mass profile of esterified N- glycans present in BSSL further permitted the more detailed studies through collision-induced dissociation (CID) and sequential exoglycosidase cleavages. The N- glycan structures were elucidated to be complex/dibranched, fucosylated/complex/dibranched, monosialylated/complex/dibranched, and monosialylated/fucosylated/dibranched entities.  (+info)

Gas-liquid chromatography of the heptafluorobutyrate derivatives of the O-methyl-glycosides on capillary columns: a method for the quantitative determination of the monosaccharide composition of glycoproteins and glycolipids. (3/5581)

We have developed a method involving the formation of hepta-fluorobutyrate derivatives of O-methyl-glycosides liberated from glycoproteins and glycolipids following methanolysis. The stable derivatives of the most common monosaccharides of these glycoconjugates (Ara, Rha, Xyl, Fuc, Gal, Man, Glc, GlcNAc, GalNAc, Neu5Ac, KDN) can be separated and quantitatively and reproducibly determined with a high degree of sensitivity level (down to 25 pmol) in the presence of lysine as an internal standard. The GlcNAc residue bound to Asn in N-glycans is quantitatively recovered as two peaks. The latter were easily distinguished from the other GlcNAc residues of N-glycans, thus allowing a considerable improvement of the data on structure of N-glycans obtained from a single carbohydrate analysis. The most common contaminants present in buffers commonly used for the isolation of soluble or membrane-bound glycoproteins (SDS, Triton X-100, DOC, TRIS, glycine, and polyacrylamide or salts, as well as monosaccharide constituents of proteoglycans or degradation products of nucleic acids) do not interfere with these determinations. A carbohydrate analysis of glycoproteins isolated from a SDS/PAGE gel or from PDVF membranes can be performed on microgram amounts without significant interferences. Since fatty acid methyl esters and sphingosine derivatives are separated from the monosaccharide peaks, the complete composition of gangliosides can be achieved in a single step starting from less than 1 microg of the initial compound purified by preparative Silicagel TLC. Using electron impact ionization mass spectrometry, reporter ions for the different classes of O-methyl-glycosides (pentoses, deoxy-hexoses, hexoses, hexosamines, uronic acids, sialic acid, and KDN) allow the identification of these compounds in very complex mixtures. The mass of each compound can be determined in the chemical ionization mode and detection of positive or negative ions. This method presents a considerable improvement compared to those using TMS derivatives. Indeed the heptafluorobutyrate derivatives are stable, and acylation of amino groups is complete. Moreover, there is no interference with contaminants and the separation between fatty acid methyl-esters and O-methyl glycosides is achieved.  (+info)

An improved method for the structural profiling of keratan sulfates: analysis of keratan sulfates from brain and ovarian tumors. (4/5581)

A previously developed method for the structural fingerprinting of keratan sulfates (Brown et al., Glycobiology, 5, 311-317, 1995) has been adapted for use with oligosaccharides fluorescently labeled with 2-aminobenzoic acid following keratanase II digestion. The oligosaccharides are separated by high-pH anion-exchange chromatography on a Dionex AS4A-SC column. This methodology permits quantitative analysis of labeled oligosaccharides which can be detected at the sub-nanogram ( approximately 100 fmol) level. Satisfactory calibration of this method can be achieved using commercial keratan sulfate standards. Keratan sulfates from porcine brain phosphocan and human ovarian tumors have been examined using this methodology, and their structural features are discussed.  (+info)

The sialylation of bronchial mucins secreted by patients suffering from cystic fibrosis or from chronic bronchitis is related to the severity of airway infection. (5/5581)

Bronchial mucins were purified from the sputum of 14 patients suffering from cystic fibrosis and 24 patients suffering from chronic bronchitis, using two CsBr density-gradient centrifugations. The presence of DNA in each secretion was used as an index to estimate the severity of infection and allowed to subdivide the mucins into four groups corresponding to infected or noninfected patients with cystic fibrosis, and to infected or noninfected patients with chronic bronchitis. All infected patients suffering from cystic fibrosis were colonized by Pseudomonas aeruginosa. As already observed, the mucins from the patients with cystic fibrosis had a higher sulfate content than the mucins from the patients with chronic bronchitis. However, there was a striking increase in the sialic acid content of the mucins secreted by severely infected patients as compared to noninfected patients. Thirty-six bronchial mucins out of 38 contained the sialyl-Lewis x epitope which was even expressed by subjects phenotyped as Lewis negative, indicating that at least one alpha1,3 fucosyltransferase different from the Lewis enzyme was involved in the biosynthesis of this epitope. Finally, the sialyl-Lewis x determinant was also overexpressed in the mucins from severely infected patients. Altogether these differences in the glycosylation process of mucins from infected and noninfected patients suggest that bacterial infection influences the expression of sialyltransferases and alpha1,3 fucosyltransferases in the human bronchial mucosa.  (+info)

Inhibition of L-selectin-mediated leukocyte rolling by synthetic glycoprotein mimics. (6/5581)

Synthetic carbohydrate and glycoprotein mimics displaying sulfated saccharide residues have been assayed for their L-selectin inhibitory properties under static and flow conditions. Polymers displaying the L-selectin recognition epitopes 3',6-disulfo Lewis x(Glc) (3-O-SO3-Galbeta1alpha4(Fucalpha1alpha3)-6-O-SO3-Glcbeta+ ++-OR) and 3',6'-disulfo Lewis x(Glc) (3, 6-di-O-SO3-Galbeta1alpha4(Fucalpha1alpha3)Glcbeta-OR) both inhibit L-selectin binding to heparin under static, cell-free binding conditions with similar efficacies. Under conditions of shear flow, however, only the polymer displaying 3',6-disulfo Lewis x(Glc) inhibits the rolling of L-selectin-transfected cells on the glycoprotein ligand GlyCAM-1. Although it has been shown to more effective than sialyl Lewis x at blocking the L-selectin-GlyCAM-1 interaction in static binding studies, the corresponding monomer had no effect in the dynamic assay. These data indicate that multivalent ligands are far more effective inhibitors of L-selectin-mediated rolling than their monovalent counterparts and that the inhibitory activities are dependent on the specific sulfation pattern of the recognition epitope. Importantly, our results indicate the L-selectin specificity for one ligand over another found in static, cell-free binding assays is not necessarily retained under the conditions of shear flow. The results suggest that monovalent or polyvalent carbohydrate or glycoprotein mimetics that inhibit selectin binding in static assays may not block the more physiologically relevant process of selectin-mediated rolling.  (+info)

Novel proteoglycan linkage tetrasaccharides of human urinary soluble thrombomodulin, SO4-3GlcAbeta1-3Galbeta1-3(+/-Siaalpha2-6)Galbeta1-4Xyl. (7/5581)

O-linked sugar chains with xylose as a reducing end linked to human urinary soluble thrombomodulin were studied. Sugar chains were liberated by hydrazinolysis followed by N-acetylation and tagged with 2-aminopyridine. Two fractions containing pyridylaminated Xyl as a reducing end were collected. Their structures were determined by partial acid hydrolysis, two-dimensional sugar mapping combined with exoglycosidase digestions, methylation analysis, mass spectrometry, and NMR as SO4-3GlcAbeta1-3Galbeta1-3(+/-Siaalpha2-6)Galbeta1+ ++-4Xyl. These sugar chains could bind to an HNK-1 monoclonal antibody. This is believed to be the first example of a proteoglycan linkage tetrasaccharide with glucuronic acid 3-sulfate and sialic acid.  (+info)

The role of homophilic binding in anti-tumor antibody R24 recognition of molecular surfaces. Demonstration of an intermolecular beta-sheet interaction between vh domains. (8/5581)

The murine antibody R24 and mouse-human Fv-IgG1(kappa) chimeric antibody chR24 are specific for the cell-surface tumor antigen disialoganglioside GD3. X-ray diffraction and surface plasmon resonance experiments have been employed to study the mechanism of "homophilic binding," in which molecules of R24 recognize and bind to other molecules of R24 though their heavy chain variable domains. R24 exhibits strong binding to liposomes containing disialoganglioside GD3; however, the kinetics are unusual in that saturation of binding is not observed. The binding of chR24 to GD3-bearing liposomes is significantly weaker, suggesting that cooperative interactions involving antibody constant regions contribute to R24 binding of membrane-bound GD3. The crystal structures of the Fabs from R24 and chR24 reveal the mechanism for homophilic binding and confirm that the homophilic and antigen-binding idiotopes are distinct. The homophilic binding idiotope is formed largely by an anti-parallel beta-sheet dimerization between the H2 complementarity determining region (CDR) loops of two Fabs, while the antigen-binding idiotope is a pocket formed by the three CDR loops on the heavy chain. The formation of homophilic dimers requires the presence of a canonical conformation for the H2 CDR in conjunction with participation of side chains. The relative positions of the homophilic and antigen-binding sites allows for a lattice of GD3-specific antibodies to be constructed, which is stabilized by the presence of the cell membrane. This model provides for the selective recognition by R24 of cells that overexpress GD3 on the cell surface.  (+info)

OTHER GLYCAN DEGRADATION GlcNAc GlcNAc Fuc Neu5Ac Gal Asn α2 β1 β1 Man α1 GlcNAc Neu5Ac Gal α2 β1 β1 Man α1 β1 GlcNAc Man β1 β1 GlcNAc β1 α1 Fuc α1 Neu5Ac α2 Neu5Ac α2 Gal β1 Neu5Ac α2 Neu5Ac α2 GalNAc Neu5Ac α2 Neu5Ac α2 Neu5Ac α2 β1 Gal β1 Glc β1 ceramide Ganglioside N-glycan N-Glycan ...
According to National Geographic, the inner core of the Earth ranges between 9,000 and 13,000 degrees Fahrenheit. This is approximately as high as the temperature of the suns...
H. Casajus, S. Tranchimand, D. Wolbert, C. Nugier-Chauvin, S. Cammas-Marion, J. Appl. Polym. Sci., 2016, DOI: 10.1002/app.44604.. • A. Pennec, R. Daniellou, P. Loyer, C. Nugier-Chauvin, V. Ferrieres Carbohydr. Res. 2015, 402, 50-55.. • A. Pennec, L. Legentil, L. Herrera-Estrella, V. Ferrières, A.-L. Chauvin, C. Nugier-Chauvin, Green Chem., 2014, 16, 3803-3809.. • I. Chlubnová, B. Sylla, C. Nugier-Chauvin, R. Daniellou, L. Legentil, B. Králová, V. Ferrières Nat. Prod. Rep., 2011, 28, 937-952.. • I. Chlubnová, D. Filipp, V. Spiwok, H. Dvořáková, R. Daniellou, C. Nugier-Chauvin, B. Králová, V. Ferrières Org. Biomol. Chem., 2010, 8, 2092-2102.. Invited talks at Conferences: ...
The N-linked carbohydrate chains of recombinant human erythropoietin expressed in CHO cells were quantitatively released with peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F, separated from the remaining O-glycoprotein by gel-permeation chromatography, and subsequently fractionated via FPLC on Mono Q, HPLC on Lichrosorb-NH2 and high-pH anion-exchange chromatography on CarboPac PA1. The purified sialylated oligosaccharides were ... read more analyzed by one-dimensional and two-dimensional 500-MHz 1H-NMR spectroscopy. When necessary, oligosaccharides were treated with endo-beta-galactosidase (and N-acetyl-beta-glucosaminidase) followed by 1H-NMR analysis of the incubation products, to obtain additional structural information. Di-, tri-, tri- and tetraantennary, N-acetyllactosamine-type oligosaccharides occur which can be completely (major) or partially (minor) sialylated. Three different types of alpha2-3-linked sialic acids are present, namely, N-acetylneuraminic acid (95%), ...
Targeting tumor-associated carbohydrate antigens: a phase I study of a carbohydrate mimetic-peptide vaccine in stage IV breast cancer subjects
This application note describes a rapid and robust high-performance anion-exchange chromatography with pulsed amperometric detection method for the accurate determination of common sugars in acid-hydrolyzed biomass samples with high carbohydrate concentrations.
The oligosaccharide chains of microheterogeneous bovine pancreatic DNAases were characterized by the lectin-nitrocellulose sheet method. The active fractions of the DNAases from column chromatography showed four major and several minor spots on a two-dimensional polyacrylamide gel. They were transferred on to nitrocellulose sheets and treated with glycosidases (neuraminidase, endo-beta-N-acetyl glucosaminidase H or F, or peptide N-glycosidase F) and treated with peroxidase-coupled lectins (concanavalin A, Ricinus communis agglutinin or wheat-germ agglutinin). From the results, the most probable oligosaccharide types were proposed to be as follows: the four major spots contained components which had high-mannose type or hybrid-type oligosaccharides, such as those susceptible to endo-beta-N-acetylglucosaminidase H. In addition, spot 1 contained a complex-type biantennary oligosaccharide without sialic acid and spot 3 contained a tri- or tetra-antennary complex-type oligosaccharide with sialic ...
Carbohydrate antigens recognized by natural or preformed and elicited antibodies are central to transplantation/transfusion rejection across ABO blood group and species (xenotransplantation) barriers and are also promising candidates for cancer immunotherapy (Ramsland 2005). The key carbohydrate determinants (epitopes) recognized by antibodies are synthesized by a series of intracellular glycosyltransferases and are expressed on the surface of cells as glycolipids and glycoproteins. Often the minimal carbohydrate epitopes are located at the terminal end of more complex oligosaccharide chains, which result in these epitopes being displayed at a wide range of surface densities and contexts (e.g., glycolipids or glycoproteins). For example, many tumor-associated carbohydrate antigens are broadly expressed at very high densities on the cell surface of primary and metastatic tumors, but the same carbohydrates occur at much lower levels and are typically restricted to a few cell types in healthy ...
There are a large number of enzymes that are capable of modifying carbohydrates or carbohydrate derivatives, and that may be used in various analytical methods. The hydrolytic enzymes, which break glycosidic linkages, are useful in the study of disaccharide or polysaccharide structure and in methods for quantitation (Table 9.2). Such enzymes will hydrolyse the glycosidic linkages between the monosaccharide residues and release the individual components for further analysis. The enzyme is chosen bearing in mind the nature of the glycosidic linkage involved, which may not be unique to one particular disaccharide or polysaccharide. Thus a-glucosidase will hydrolyse both the a(l-»4) linkage of maltose and the a( 1-»2) linkage of sucrose, resulting in the release of glucose in both cases.. ...
Export GlyConnect composition list related to tissue with id 363 Hex:1 HexNAc:2 # id : 495 / avg mass :586.5477 Hex:3 HexNAc:2 # id : 507 / avg mass :910.8325 Hex:3 HexNAc:2 dHex:1 # id : 740 / avg mass :1056.9755 Hex:3 HexNAc:3 # id : 574 / avg mass :1114.0275 Hex:3 HexNAc:3 dHex:1 # id : 202 / avg mass :1260.1705 Hex:3 HexNAc:4 # id : 500 / avg mass :1317.2225 Hex:3 HexNAc:4 NeuAc:1 # id : 64 / avg mass :1608.4804 Hex:3 HexNAc:4 dHex:1 # id : 742 / avg mass :1463.3655 Hex:3 HexNAc:4 dHex:1 NeuAc:1 # id : 328 / avg mass :1754.6234 Hex:3 HexNAc:6 dHex:1 NeuAc:2 # id : 624 / avg mass :2452.2713 Hex:4 HexNAc:2 # id : 652 / avg mass :1072.9749 Hex:4 HexNAc:2 dHex:1 # id : 345 / avg mass :1219.1179 Hex:4 HexNAc:3 # id : 452 / avg mass :1276.1699 Hex:4 HexNAc:3 dHex:1 # id : 713 / avg mass :1422.3129 Hex:4 HexNAc:3 dHex:1 NeuAc:1 # id : 286 / avg mass :1713.5708 Hex:4 HexNAc:4 # id : 648 / avg mass :1479.3649 Hex:4 HexNAc:4 NeuGc:1 # id : 133 / avg mass :1786.6222 Hex:4 HexNAc:4 NeuAc:1 # id : 547 / ...
Export GlyConnect composition list related to peptides with id 1571 Hex:3 HexNAc:2 dHex:1 # id : 740 / avg mass :1056.9755 Hex:3 HexNAc:3 dHex:1 # id : 202 / avg mass :1260.1705 Hex:3 HexNAc:3 dHex:2 # id : 357 / avg mass :1406.3135 Hex:3 HexNAc:4 dHex:1 # id : 742 / avg mass :1463.3655 Hex:3 HexNAc:5 dHex:1 # id : 538 / avg mass :1666.5605 Hex:4 HexNAc:2 dHex:1 # id : 345 / avg mass :1219.1179 Hex:4 HexNAc:3 dHex:1 # id : 713 / avg mass :1422.3129 Hex:4 HexNAc:3 dHex:1 NeuAc:1 # id : 286 / avg mass :1713.5708 Hex:4 HexNAc:3 dHex:2 # id : 160 / avg mass :1568.4559 Hex:4 HexNAc:3 dHex:3 # id : 985 / avg mass :1714.5989 Hex:4 HexNAc:4 NeuAc:1 # id : 547 / avg mass :1770.6228 Hex:4 HexNAc:4 dHex:1 # id : 350 / avg mass :1625.5079 Hex:4 HexNAc:4 dHex:1 NeuAc:1 # id : 173 / avg mass :1916.7658 Hex:4 HexNAc:4 dHex:2 # id : 213 / avg mass :1771.6509 Hex:4 HexNAc:4 dHex:2 NeuAc:1 # id : 685 / avg mass :2062.9088 Hex:4 HexNAc:4 dHex:3 # id : 751 / avg mass :1917.7939 Hex:4 HexNAc:6 # id : 53 / avg mass ...
Read independent reviews on Thermo Scientific™ CarboPac® PA1 Carbohydrate Column from Thermo Fisher Scientific on SelectScience
UPLC, UHPLC, and HPLC-based C18 columns that assist scientists with the analysis and quantitation of important glycan derived components.
Heparin Hexasaccharide MS Standard 7. The Heparin Hexasaccharide MS Standard 7 is a MS standard of defined sequence and structure. This highly purified hexasaccharide with seven sulfates is prepared from a digest of porcine mucosal heparin using Bacteroides Heparinase I
Oxygen is the most abundant element in the planet, so it is not unreasonable to expect that it might be one of the dominant light elements in the core.. The composition of the Earths core remains a mystery. Scientists know that the liquid outer core consists mainly of iron, but it is believed that small amounts of some other elements are present as well.. Now, a new research from a team including Yingwei Fei from Carnegies Geophysical Laboratory has revealed that oxygen does not have a major presence in the outer core.. This has major implications for our understanding of the period when the Earth formed through the accretion of dust and clumps of matter.. According to current models, in addition to large amounts of iron, the Earths liquid outer core contains small amounts of so-called light elements, possibly sulfur, oxygen, silicon, carbon, or hydrogen.. The team provides new experimental data that narrow down the identity of the light elements present in Earths outer core.. High-speed ...
H. Kunz, B. Liebe, W. Dippold, C. Claus, J. Habermann, U. Sprengard, K. Peilstöcker, M. Rösch, in: Medicinal Chemistry into the Millennium (M. M. Campbell, I. S. Blagbrough, Hrsg.), The Royal Society of Chemistry, Cambridge, UK, 2001, 245-257. 263.Applications of Ugi Reactions in Synthesis of Divalent Neoglycoconjugates: Evidence that the Sugars are Presented in Testricted Conformation ...
Supplier pricing and chemical structure of high purity Man(a1-2)-Man(a1-2)-Man(a1-3)-[Man(a1-6)-Man(a1-6)]-Man(b1-4)-GlcNAc(b1-4)-GlcNAc from Omicron Biochemicals, Inc.
Supplier pricing and chemical structure of high purity Man(a1-2)-Man(a1-3)-[Man(a1-6)-Man(a1-6)]-Man(b1-4)-GlcNAc(b1-4)-GlcNAc from Omicron Biochemicals, Inc.
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the glycosyltransferase 31 family. The encoded protein synthesizes GalNAc:beta-1,3GlcNAc, a novel carbohydrate structure, on N- and O-glycans. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Mar 2013 ...
GlycanMass is a tool which allows to calculate the mass of an oligosaccharide structure [Mass values / Disclaimer]. Note: You can use GlycoMod to predict the possible oligosaccharide structures that occur on proteins from their experimentally determined masses ...
Visit the page of myhomeworkhelp.com and check out their Oligosaccharides Assignment Help team which is ready 24/7 to provide assistance to students.
A. During early development, a snake embryo was exposed to a toxin that interferes with normal migration and differentiation of mesoderm. Name the major structures of the integument most affected by this exposure and how each.
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By the end of this section, you will be able to: Identify the anatomical and functional divisions of the nervous system Relate the functional and struc
Hello everyone, this is part 2 of this series. In the previous part, we discussed how to create a new project and set the home page. In this part, we'll discuss how to perform navigation in Xamarin forms.
Roundtables are designed to encourage scholarly discussion and exchange of views around a subject of relevance and should have a minimum of three presenters, including a chair.. Please provide a single abstract for the roundtable of no more than 200 words including the following structure:. ...
இயற்கையில் இம்மெத்தாக்சி சேர்மங்கள் அதிகமாக காணப்படாமல் இருப்பதாலேயே முக்கியத்துவம் பெறுகின்றன. அவை அமினோ அமிலங்களில் இருப்பதில்லை. இந்த வேற்றணு வளையச் சேர்மங்கள் மரபுக்குறியீட்டிலும் காணப்படவில்லை கொழுமியத்திலும் காணப்படவில்லை. இருந்தாலும் இவை O-மெத்திலேற்ற பிளவோனாய்டு எனப்படும் தாவர நிறமிகளின் பகுதிப்பொருளாக உள்ளது. இவை பீனால்கள் மீது செயல்படுகின்றன. உதாரணம்: ...
Phospholipid patterns of 15 representative strains of the genus Amycolatopsis were recorded by two-dimensional thin-layer chromatography. The structure analysis of the isolated phospholipids was verified by fast atom bombardment-mass spectroscopy. The positive- and negative-ion spectra of the partially purified phospholipid fractions qualitatively reflect their distinctive composition. All strains contained diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylinositol. Two different types of phosphatidylethanolamine and phosphatidylmethylethanolamine were detected, viz., compounds with or without hydroxy fatty acids. These phospholipid patterns underline the integrity of the genus. Fast atom bombardment-mass spectrometry analysis of phospholipid patterns may serve as an aid for differentiation of bacterial species.
TY - JOUR. T1 - Chemical reactions in fast atom bombardment mass spectrometry. AU - Vékey, K.. AU - Zerilli, Luigi F.. PY - 1991. Y1 - 1991. N2 - Examples of various chemical reactions occurring in the matrix or in the selvedge region in fast atom bombardment (FAB) mass spectrometry are discussed. These are categorized as oxidations and reductions; substitutions; clusterings and additions; and sample decomposition or transformation. Some reactions observed showed significant time behaviour and in one case it was possible to determine rate constants. These data suggest that chemical reactions can be accelerated significantly by fast atom bombardment.. AB - Examples of various chemical reactions occurring in the matrix or in the selvedge region in fast atom bombardment (FAB) mass spectrometry are discussed. These are categorized as oxidations and reductions; substitutions; clusterings and additions; and sample decomposition or transformation. Some reactions observed showed significant time ...
Equine chorionic gonadotropin (eCG) and lutropin (eLH) are heterodimeric glycoprotein hormones which are synthesized in the placenta and pituitary, respectively. The beta subunits of eCG and eLH, like their alpha subunits, arise from a single gene and have identical amino acid sequences. In contrast, the beta subunits of CG and LH in primates arise from different genes and differ in sequence. We have examined the structures of the Asn-linked oligosaccharides on eCG and eLH. eCG bears di- and tri-branched Asn-linked oligosaccharides terminating with Sia alpha 2,3 or 6Gal beta 1,4GlcNAc. In contrast, , 72% of the Asn-linked oligosaccharides on eLH have 1 or 2 branches terminating with the sequence SO4-4-GalNAc beta 1,4GlcNAc. The nonsulfated oligosaccharides on eLH are neutral (6% of the total) or have branches terminating with sialic acid-Gal (22% of the total). Since the alpha and beta subunits of eCG and eLH both contain the tripeptide motif, Pro-Xaa-Arg/Lys, recognized by the glycoprotein ...
Brown, Gavin M. and Huckerby, Thomas N. and Morris, Haydn G. and Abram, Beverly L. and Nieduszynski, Ian A. (1994) Oligosaccharides Derived from Bovine Articular Cartilage Keratan Sulfates after Keratanase II Digestion: Implications for Keratan Sulfate Structural Fingerprinting. Biochemistry, 33 (16). pp. 4836-4846. ISSN 1520-4995 Full text not available from this repository ...
Heparin and heparan sulfate bind a host of basic proteins that take advantage of the sugars dense structural information. The significance of these interactions in various aspects of development, physiology, and disease stimulated keen interest in evaluating structure-activity relationships. The well-defined heparin and heparan sulfate oligosaccharides needed for these studies can be mainly accessed by chemical synthesis and, more recently by chemoenzymatic means. The various synthetic strategies available to chemical synthesis have recently enabled the acquisition of several regular and irregular sequences, including a number of dodecasaccharides, through improved coupling methods and judicial protecting group manipulations. Controlled chain elongation and critical application of modification enzymes allowed the generation of well-defined constructs via chemoenzymatie synthesis. Investigations of various protein interactions with the synthetic constructs delivered valuable informatio
Identification of the methyl phosphate substituent at the non-reducing terminal mannose residue of the O-specific polysaccharides of Klebsiella pneumoniae O3, Hafnia alvei PCM 1223 and Escherichia coli O9/O9a LPS
The fast atom bombardment (FAB) mass spectra of bouvardin (1) 6-O-methylbouvardin (2), deoxybouvardin (3) and a synthetic analog (4) have been examined. The spectra of the bicyclic compounds 1-3 display site-directed fragmentations resulting from the presence of a phenolic bridged tyrosine moiety un …
We have analyzed the O-antigen polysaccharide of the previously uncharacterized Escherichia coli strain TD2158 which is a host of bacteriophage HK620. This bacteriophage recognizes and cleaves the polysaccharide with its tailspike protein (TSP). The polysaccharide preparation as well as oligosaccharides obtained from HK620TSP endoglycosidase digests were analyzed with NMR spectroscopy. Additionally, sugar analysis was performed on the O-antigen polysaccharide and MALDI-TOF MS was used in oligosaccharide analysis. The present study revealed a heterogeneous polysaccharide with a hexasaccharide repeating unit of the following structure: alpha-D-Glcp-(1 -, 6) vertical bar vertical bar 2)-alpha-L-Rhap-(1 -, 6)-alpha-D-Glcp-(1 -, 4)-alpha-D-Galp-(1 -, 3)-alpha-D-GlcpNAc- (1 -,vertical bar beta-D-Glcp/beta-D-GlcpNAc-(1 -, 3) A repeating unit with a D-GlcNAc substitution of D-Gal has been described earlier as characteristic for serogroup O18A1. Accordingly, we termed repeating units with D-Glc ...
Eleven tested anions were able to form adducts with neutral oligosaccharides at low cone voltage in negative ion mode electrospray mass spectrometry. Among them, fluoride and acetate have the abilities to significantly enhance the absolute abundance of [M-H]- for neutral oliogosaccharides. The chloride adduct has the best stability among all the adduct species investigated. For the above three anions, CID of adduct species may be used for structural determination of neutral oligosaccharides. In the presence of F- and Ac-, simultaneous detection of acidic oligosaccharides and neutral oligosaccharides was achieved. The ratio of Cl- : non-Cl-containing product ions obtained in CID spectra of chloride adducts of disaccharides was used to differentiate anomeric configurations of disaccharides. Density functional theory (DFT) was employed to evaluate the optimized structures of chloride adducts of disaccharides. The formation and decomposition of chloride adducts with oligosaccharides of different
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Purchase Neoglycoconjugates, Part B: Biomedical Applications, Volume 247 - 1st Edition. Print Book & E-Book. ISBN 9780121821487, 9780080883601
article{fb86bc26-01e6-493b-b144-14ab87d1a4c7, abstract = {The synthesis of the Linear B type 2 trisaccharide (Galalpha3Galbeta4GlcNAc-betaOTMSEt) and the corresponding 2-carboxyethyl beta-thioglycoside is described, as well as coupling of the latter to Sepharose.}, author = {Dahmen, J and Magnusson, Göran and Hansen, HC}, issn = {0732-8303}, keyword = {xenotransplantation,glycoconjugate,glycoside synthesis,thioglycoside}, language = {eng}, number = {3}, pages = {189--199}, publisher = {Marcel Dekker}, series = {Journal of Carbohydrate Chemistry}, title = {Synthesis of the Linear B type 2 trisaccharide Gal alpha 3Gal beta 4GlcNAc beta OTMSEt, and coupling of the corresponding 2-carboxyethyl beta-thioglycoside to Sepharose}, url = {http://dx.doi.org/10.1081/CAR-120004332}, volume = {21}, year = {2002 ...
The structures of hepta- to tetradeca-saccharides, generated by digestion of wheat-endosperm arabinoxylan with endo-(1-,4)-beta-D-xylanase, and isolated by gel-permeation chromatography on Bio-Gel P-6 and high-performance anion-exchange chromatography with pulsed amperometric detection (h.p.a.e.-p.a.d.), were elucidated using monosaccharide and methylation analysis, f.a.b.-m.s., and 1H-n.m.r. spectroscopy. The structures identified had two branching elements, -,4)[alpha-L-Araf-(1-,3)]-beta-D-Xylp-(1-, ... read more and/or -,4)[alpha-L-Araf-(1-,2)][alpha-L-Araf-(1-,3)]-beta-D-Xylp-(1-,, directly connected to each other in all four possible combinations. The h.p.a.e.-p.a.d. elution pattern showed that these combinations are not present in equal amounts. Also, compounds containing two 2,3-branched beta-D-Xylp residues separated by one or two unbranched beta-D-Xylp residues were found, and the presence of a tetradecasaccharide containing three 2,3-branched beta-D-Xylp residues was established. show ...
The primary structure of the major surface glycoconjugate of Leishmania donovani parasites, a lipophosphoglycan, has been further characterized. The repeating PO4-6Galp beta 1-4Man disaccharide units, which are a salient feature of the molecule, are shown to terminate with one of several neutral structures, the most abundant of which is the branched trisaccharide Galp beta 1-4(Manp alpha 1-2)Man. The phosphosaccharide core of lipophosphoglycan, which links the disaccharide repeats to a lipid anchor, contains 2 phosphate residues. One of the core phosphates has previously been localized on O-6 of the galactosyl residue distal to the lipid anchor; the second phosphate is now shown to be on O-6 of the mannosyl residue distal to the anchor and to bear an alpha-linked glucopyranosyl residue. Also, the anomeric configuration of the unusual 3-substituted Galf residue in the phosphosaccharide core is established as beta. The complete structure of the core is thus PO4-6Galp alpha 1-6Galp alpha 1-3Galf ...
The primary structure of the major surface glycoconjugate of Leishmania donovani parasites, a lipophosphoglycan, has been further characterized. The repeating PO4-6Galp beta 1-4Man disaccharide units, which are a salient feature of the molecule, are shown to terminate with one of several neutral structures, the most abundant of which is the branched trisaccharide Galp beta 1-4(Manp alpha 1-2)Man. The phosphosaccharide core of lipophosphoglycan, which links the disaccharide repeats to a lipid anchor, contains 2 phosphate residues. One of the core phosphates has previously been localized on O-6 of the galactosyl residue distal to the lipid anchor; the second phosphate is now shown to be on O-6 of the mannosyl residue distal to the anchor and to bear an alpha-linked glucopyranosyl residue. Also, the anomeric configuration of the unusual 3-substituted Galf residue in the phosphosaccharide core is established as beta. The complete structure of the core is thus PO4-6Galp alpha 1-6Galp alpha 1-3Galf ...
The oligosaccharides ID in the library are used to refer to specific oligosaccharides for their tandem MS/MS fingerprints and structures and were obtained using their sequential monomeric composition. For example, the oligosaccharide with ID 11100 is made of 1 hexose + 1 HexNAc + 1 fucose + 0 NeuAc + 0 NeuGC.. ...
The Global Soybean Oligosaccharides report provides an elite resource to evaluate the Soybean Oligosaccharides market and support the strategic and preemptive decision-making. The report divulges the matter-of-fact statistics and in-detail examination of the market. It offers a basic layout of the Soybean Oligosaccharides industry, consisting of classifications, applications, definitions, and industry chain structure. It also encompasses a thorough inference of the market and embodies significant insights, facts, and industry-corroborated statistics of the global Soybean Oligosaccharides market.. Furthermore, the report also elaborates several factors concerning the Soybean Oligosaccharides market, comprising key trends, standardization, deployment models, future roadmap, strategies, technologies, value chain, opportunities, ecosystem player profiles, drivers, operator case studies, regulatory landscape, and challenges. Moreover, it also presents an outline regarding the Soybean Oligosaccharides ...
This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase protein family. The encoded enzyme is involved in the biosynthesis of poly-N-acetyllactosamine chains and prefers lacto-N-neotetraose as a substrate. It is a type II transmembrane protein ...
Sialyl Lewis A (sLeA, also known as CA19-9), a tetrasaccharide selectively and highly expressed on advanced adenocarcinomas including colon, stomach, and pancreatic cancers, has long been considered as an attractive target for active and passive vaccination. While progress in antibodies targeting tumor-associated protein antigens resulted in an impressive array of therapeutics for cancer treatment, similar progress in exploiting tumor-associated carbohydrate antigens, such as sLeA, has been hampered by the lack of a detailed understanding of the singular characteristics of these antigens. We have addressed this issue by analyzing antibodies derived from patients immunized with an sLeA/KLH vaccine. These antibodies were engineered to mediate tumor clearance in vivo in preclinical models through Fc-FcγR interactions. However, in contrast to protein antigens in which hFcγRIIIA engagement was both necessary and sufficient to mediate tumor clearance in both preclinical and clinical settings, a ...
Sulfotransferase that utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. Acts on the non-reducing terminal GlcNAc of short carbohydrate substrates. However, it does not transfer sulfate to longer carbohydrate substrates that have poly-N-acetyllactosamine structures. Has no activity toward keratan. Not involved in generating HEV-expressed ligands for SELL. Its substrate specificity may be influenced by its subcellular location ...
keratanase II: an endo-beta-N-acetylglucosaminidase; cleaves the beta(1-3)-glycosidic bond of a fucosylated 6-O-sulfated N-acetylglucosamine
In the few years since the beginning of glycomics in 21th century, many achievements have been made in the study of glycomics, especially in the discovery of sugar genes and the elucidation of the new structure of sugar chain. Although glycomics research has made remarkable achievements in these fields, there are many unknown intermediate links between sugar genes and sugar chains, sugar chains and glycan binding proteins, and there is a general lack of detailed research on the relationship between structure and function.. These fields are not only related to the progress of glycomics itself, but also directly related to the progress of proteome planning, so it has become a major bottleneck in the development of life science.. The advancement of analytical techniques has always been a direct driving force in the development of glycomics. It is the rapid development and wide application of modern instrument analysis technology that lead to the overall prosperity of glycomics research, and inject ...
The MUC-1 antigen is a membrane-anchored mucin-type glycoprotein present in malignant and normal epithelial cells of certain organs, e.g. breast, lung, ovary, pancreas and colon (1). The apoprotein of the MUC-1 mucin contains a transmembrane domain, a cytoplasmic domain, and an extracellular carbohydrate rich domain. The extracellular domain is characterized by polymorphism with respect to the number of a 20 amino acid tandem repeat (VNTR polymorphism). The CanAg CA15-3 EIA is based on two mouse monoclonal antibodies, Ma695 as catcher antibody recognizing a sialylated carbohydrate epitope expressed on the MUC-1 antigen and Ma552 as tracer antibody targeting the PDTRPAPG region of the protein core (2-5).The MUC-1 breast cancer mucin (CA15-3 antigen) is secreted from tumor cells and is a well-established serological marker for monitoring the clinical course of breast cancer patients (6). ...
Thermo Fisher Scientific has developed a high-performance anion-exchange chromatography with pulsed amperometric detection method, based on AOAC Official Method 2011. Click to read more...
RNase B is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A and which contains carbohydrate to the extent of 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is consequently considered to be a carbohydrate derivative of RNase A. ( Tarentino, A., Plummer, J., and Maley, F.: Studies on the Oligosaccharide Sequence of Ribonuclease B, J. Biol. Chem., 245, 4150 (1970 ...
The present study tested and confirmed the hypothesis that autoantibodies to aberrant O-glycopeptide epitopes represent a fruitful source of sensitive biomarkers for early detection of cancer. Cancer-associated IgG autoantibodies to several O-glycopeptide epitopes were identified in MUC1, whereas IgG antibodies to peptide epitopes were not detected. The study therefore clearly supports that autoantibody biomarker discovery strategies should include aberrant posttranslational modifications for greatest success. Chemoenzymatic synthesis of cancer-associated O-glycopeptides in combination with a microarray platform was shown to be a feasible strategy for broader analysis of the entire cancer O-glycopeptidome.. Initially, we found an absence of immature nonsialylated MUC1 and MUC16 glycoforms in serum of cancer patients with elevated mucin levels, suggesting that these glycoforms are removed by immune cells or scavenger receptors recognizing immature uncapped glycans. This is in agreement with Varki ...
An estimate of the relative abundance of the various O-glycans was determined from nanoLC-ESI-IT-MS analysis of trypsin-generated (glyco)peptides. It should be noted that because the signal of the monosialylated O-glycopeptide in triply charged state overlapped with the doubly charged peak of the hinge repeat peptide with a putative acetylation modification in all samples, only the doubly charged signal of the monosialylated O-glycopeptide was quantified, thereby leading to an underestimation of this O-glycoform. Furthermore, it is known that glycopeptides with different glycan structures can have different response factors (35), and thus the relative abundances we measured may not accurately reflect the real ratios. In order to obtain a more reliable estimate of the percentage of the hinge repeat motif bearing an O-glycan, tryptic IgG peptides were incubated with exoglycosidases, trimming all O-glycans down to a single N-acetylhexosamine. A previous study of quantitative measurements of a ...
View Notes - 351 C4 Smith key from CHEM 351 at BYU. Chemistry 351 Fall 2008 Chapter 4 1. Assign the IUPAC name to the following structures. 0k 1,: _Jhfigg~mefi%fimfilm c, 2&4
Mgat4c (untagged) - Mouse mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme C (putative) (Mgat4c), transcript variant 1, (10ug), 10 µg.
IN STOCK 13C labeled di-sialylated, galactosylated biantennary N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications
Kilpatrick, D.C. Pusztai, A., Grant, G., Graham, C. and Ewen, S.W (1985) Tomato Lectin Resists Digestion in the Mammalian Alimentary Canal and Binds to Intestinal Villi without Deleterious Effects. FEBS Letters, 185, 299-305.
EMDEX® is a directly compressible, water-soluble tablet binder and filler. Its unique composition of 95% glucose monohydrate and different oligosaccharides derived from starch is monographed under
Shop Oligosaccharide 4-alpha-D-glucosyltransferase ELISA Kit, Recombinant Protein and Oligosaccharide 4-alpha-D-glucosyltransferase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Human Anti-Meningococcal Group A Oligosaccharides IgG negative control serum Serum Controls 600-801-ANC Human Anti-Meningococcal Group A Oligosaccharides IgG negative control serum Serum Controls 600-801-ANC
Background Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X...
Cells All organisms are made of cells. The cell is the smallest unit of living material having major structures within it allowing it to live. Some kinds of organisms are just one cell. This single cell is the organisms entire body. Many organisms are made of more than one cell.
19 janvier 2012. P,The Taleghan fault (TF) is a major active fault of the Central Alborz mountain range in Iran. Located 50 km northwestwards of Tehran, this 80-km-long fault represents one of the major structures threatening 15 million people living in the capital of Iran and the surrounding cities (e.g. (...). Lire la suite. ...
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undecaprenyl phosphate-α-L-Ara4N: 4-amino-4-deoxy-β-L-arabinosyltransferase (EC 2.4.2.43); dodecaprenyl phosphate-β-galacturonic acid: lipopolysaccharide core α-galacturonosyl transferase (EC 2.4.1.- ...
undecaprenyl phosphate-α-L-Ara4N: 4-amino-4-deoxy-β-L-arabinosyltransferase (EC 2.4.2.43); dodecaprenyl phosphate-β-galacturonic acid: lipopolysaccharide core α-galacturonosyl transferase (EC 2.4.1.- ...
For an organism to develop and function, the individual cells must exchange information, or communicate, with each other. Is it possible to learn their language and
Methods to allow the clean preparation of oligosaccharides were investigated using techniques that do not require conventional column chromatography or an aqueous work-up. The route was designed to provide rapid access to oligosaccharides and is suit
In this 3 volume collection focusing on glycomics, readers will appreciate how such discoveries were made and how such methods can be applied for readers...
In previous studies, we have shown that the highly sialylated renal anti-adhesin Podocalyxin (PC) is expressed in the developing brain [13], [14 ...
CarbohydratesSequences and topology. 44: 39-47. doi:10.1016/j.sbi.2016.11.011. PMID 27940408. Crispin M, Stuart DI, Jones EY ... Around 20% of the deposited carbohydrate structures are in unjustified energy minima. A number of carbohydrate validation web ... carbohydrate validation) Privateer (carbohydrate validation) OOPS2, part of the Uppsala Software Factory ProSA web service ... see for recommendations on carbohydrate model building and refinement and for reviews on general errors in carbohydrate ...
"Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing". Nature ... "Advancing Solutions to the Carbohydrate Sequencing Challenge". Journal of the American Chemical Society. 141 (37): 14463-14479 ... carbohydrates and their conjugates using mass spectrometry-based methods[citation needed]. Eyers' work in biological mass ...
... sequencing' carbohydrates through chemical and conformational means. Detailed studies of enzyme reactions have also provided ... "Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing". Nature ... "Advancing Solutions to the Carbohydrate Sequencing Challenge". Journal of the American Chemical Society. 141 (37): 14463-14479 ... Flitsch's research is focussed on glycobiotechnology - the study of carbohydrates in applied science and biocatalysis - the ...
Carbohydrates: A feast of structural glycobiology • Sequences and topology: Computational studies of protein-protein ... modification and breakdown of carbohydrates. His chemical and structural insight into protein-carbohydrate interactions and his ... He has made fundamental additions to our understanding of enzyme mechanism and carbohydrate biochemistry. As a direct result of ... Agirre, Jon; Davies, Gideon J; Wilson, Keith S; Cowtan, Kevin D (June 2017). "Carbohydrate structure: the rocky road to ...
Davies GJ, Sinnott ML (2008). "Sorting the diverse: The sequence‑based classifications of carbohydrate‑active enzymes" (PDF). ... CAZy was established in 1999 in order to provide online and constantly updated access to the protein sequence-based family ... The rapid evolution of high-throughput DNA sequencing has resulted in the continuing exponential growth of the CAZy database, ... "CAZy - Carbohydrate-Active enZYmes Database". Architecture et Fonction des Macromolécules Biologiques, CNRS,Université de ...
1990). "Conformer populations of L-iduronic acid residues in glycosaminoglycan sequences". Carbohydrate Research. 195 (2): 157- ... Heparin binds to AT via a specific pentasaccharide sulfation sequence contained within the heparin polymer: GlcNAc/NS(6S)-GlcA- ... It is a synthetic pentasaccharide, whose chemical structure is almost identical to the AT binding pentasaccharide sequence that ... Heparin is a member of the glycosaminoglycan family of carbohydrates (which includes the closely related molecule heparan ...
Because 3-D structures are better conserved than sequences, several of the families defined on the basis of sequence ... This classification is available on the CAZy (Carbohydrate-Active EnZymes) web site. The same three-dimensional fold is ... Henrissat B, Coutinho PM (1999). "Carbohydrate-Active Enzymes server". Cite journal requires ,journal= (help) Kolbinger F, ... "A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities". Biochem. J. 326 ...
Because 3-D structures are better conserved than sequences, several of the families defined on the basis of sequence ... This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site. The same three-dimensional fold is ... Sialyltransferase may be responsible for the synthesis of the sequence NEUAC-Alpha-2,3-GAL-Beta-1,3-GALNAC-, found on sugar ... Henrissat B, Coutinho PM (1999). "Carbohydrate-Active Enzymes server". Cite journal requires ,journal= (help) Dall'Olio and ...
The system activates as a signal sequence HlyA binds HlyB on the cell membrane. This signal sequence is an ABC transporter. The ... T1SS transports various molecules including ions, carbohydrates, drugs, proteins. The secreted molecules vary in size from the ... In SecA pathway, a chaperone trigger factor (TF) first bind to the exposed N-terminal signal sequence of the peptide chain. As ... In this pathway, SRP competes with TF and binds to the N-terminal signal sequence. Proteins from inner membrane stops the ...
Sulfation of tyrosines and carbohydrates occurs within the TGN. Other general post-translational modifications of proteins ... depending upon the signal sequence they carry. In this model, the Golgi is viewed as a set of stable compartments that work ... Protein modifications may form a signal sequence that determines the final destination of the protein. For example, the Golgi ... include the addition of carbohydrates (glycosylation) and phosphates (phosphorylation). ...
Its sequence contains two regions of tandem 19-residue repeats, where the repeat motif consists of short beta-strands separated ... This domain is suggested to be a carbohydrate-dependent haemagglutination activity site. In Bordetella pertussis, the ... Members generally have a signal sequence, then an intervening region, then the region described in this entry. Following this ...
The bacteria contain an adhesion protein for the carbohydrate sequence Gal-1,4Gal. After incubation with various amounts of the ... The genome of S. suis isolates from the outbreak were examined to see whether its DNA sequence could explain why these ...
CArbohydrate-Active EnZymes) web site.[4][8] The database provides a series of regularly updated sequence based classification ... Sequence-based classification[edit]. Sequence-based classifications are among the most powerful predictive method for ... the sequence-based families have been classified into 'clans' of related structure. Recent progress in glycosidase sequence ... the carbohydrate-active enzymes and binding proteins involved in the synthesis and degradation of complex carbohydrates ...
... or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence ... Because the fold of proteins is better conserved than their sequences, some of the families can be grouped in 'clans'. As of ... Cantarel BL, Coutinho PM, Rancurel C, Bernard T, Lombard V, Henrissat B (January 2009). "The Carbohydrate-Active EnZymes ... Henrissat B, Bairoch A (June 1996). "Updating the sequence-based classification of glycosyl hydrolases". Biochem. J. 316 (2): ...
The enzymes contain ~250-300 residues, which encode putative signal sequences and carbohydrate attachment sites. In addition, ...
Owing to the lack of a genetic blue print, carbohydrates do not have a "fixed" sequence. Instead, the sequence is largely ... as well as carbohydrate interactions. Conventional usage of the term does not currently include the treatment of carbohydrates ... 2004). Carbohydrate Polymers 55:171-177. Helenius A, Aebi M (2001) Intracellular functions of N-linked glycans. Science 291: ... For instance, just four sugars can be strung together to form more than 5 million different types of carbohydrates or nine ...
The major carbohydrate sequence linked to glycodelin-A also profusely coats the surface of schistosomes. The profile of the ... For example, human gametes are coated with carbohydrate sequences that have been implicated in the suppression of NK cell ... There are some notable examples of this mimicry or acquisition of the same carbohydrate sequences implicated in this protective ... The eu-FEDS model further suggests that specific carbohydrate sequences (oligosaccharides) are covalently linked to these ...
The sequence is divided up into codons, each of which is a particular sequence of three nucleotides and corresponds to a ... The four groups of macromolecule are nucleic acids, proteins, carbohydrates and lipids. Nucleic acids (specifically ... sequence. Sequence comparisons suggest recent horizontal transfer of many genes among diverse species including across the ... Thus a sequence of DNA codes for a particular protein that, due to the chemical properties of the amino acids it is made from, ...
The carbohydrate-active enzyme database presents a sequence-based classification of glycosyltransferases into over 90 families ... Sequence-based classification methods have proven to be a powerful way of generating hypotheses for protein function based on ... Carbohydrate chemistry Chemical glycosylation Glucuronosyltransferase Glycogen synthase Glycosyl acceptor Glycosyl donor ... The result of glycosyl transfer can be a carbohydrate, glycoside, oligosaccharide, or a polysaccharide. Some ...
ABRF Next Generation Sequencing Group (ABRF-NGS) Antibody Technology Research Group (ARG) Biomedical 'Omics Research Group ( ... Other: bioinformatics, carbohydrate analysis, differential display, recombinant protein production. Every year the Association ... 2017 Sir Shankar Balasubramanian and David Klenerman for the invention of a method of next-generation DNA sequencing which is ... Past Award Winners: 2021 - 2020 George Church for his groundbreaking research in genomic sequencing and his leadership in the ...
Kellermann J, Lottspeich F, Geiger R, Deutzmann R (1988). "Human urinary kallikrein--amino acid sequence and carbohydrate ... Complete amino acid sequence and sites of glycosylation". Int. J. Pept. Protein Res. 33 (4): 237-49. doi:10.1111/j.1399- ... 1996). "Evaluation of the extent of the binding site in human tissue kallikrein by synthetic substrates with sequences of human ... Lottspeich F, Geiger R, Henschen A, Kutzbach C (1980). "N-Terminal amino acid sequence of human urinary kallikrein homology ...
June 1998). "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones ... Protein O-GlcNAcases belong to glycoside hydrolase family 84 of the carbohydrate active enzyme classification. Homologs exist ... Among mammals, the OGA sequence is even more highly conserved. The mouse and the human have 97.8% homology. However, OGA does ... Carbohydrate Research. 341 (8): 971-82. doi:10.1016/j.carres.2006.03.004. PMID 16584714. Dong DL, Hart GW (July 1994). " ...
It contains a conserved sequence that is the reverse complement of the Shine-Dalgarno sequence of many transport and metabolic ... Broadly, it is suggested that tsRNAs regulate carbohydrate and amino acid metabolism in Burkholderia. They are more highly ... Likely targets include many genes that regulate carbohydrate transport and the degradation of aromatic amino acids. Specific ... it has been suggested that tsRNAs regulate gene expression by binding the ribosome binding sequence of multiple mRNAs. The low ...
Sequence data shows that initial viral variants in an immunologically naïve host have few glycosylation sites and shorter ... PNGSs allow for the binding of long-chain carbohydrates to the high variability regions of gp120, so the authors hypothesize ... 2008). "HIV Sequence Compendium", Los Alamos National Laboratory. Hallenberger S, Bosch V, Angliker H, Shaw E, Klenk HD, Garten ... The presence of large carbohydrate chains extending from gp120 might obscure possible antibody binding sites. The boundaries of ...
Carbohydrate sequence specificity of the mouse monoclonal antibodies that recognize crossreacting antigens on LOS and human ...
Amino acid sequence homology with the other aspartic proteinases, disulfide bond arrangement and site of carbohydrate ...
Within its sequence is a region containing tandem repeats of about 84 amino acids. This sequence is highly conserved between ... About 2-3% of its mass is carbohydrates. Aggregates of hyalin also form by associating the heads of the protein, and hyalin ... A recombinant part of this sequence was created and its adhesive properties were tested. It was found to be about as adhesive ...
Carbohydrates: Other compounds detected in the particles 3% carbohydrates.. Use as gene delivery vectors[edit]. Main article: ... Genome sequence has terminal repeated sequences; long terminal repeats (LTR) (of about 600 nt) ... The Long terminal repeat (LTR) is about 600 nt long, of which the U3 region is 450, the R sequence 100 and the U5 region some ...
Amino acid sequences of this loop play an important role in the carbohydrate-binding specificities of these lectins. These ... despite differences in the primary sequences. The carbohydrate-binding sites of these lectins consist of two conserved amino ... X-ray studies have shown that the folding of the polypeptide chains in the region of the carbohydrate-binding sites is also ... Leguminous lectins consist of two or four subunits, each containing one carbohydrate-binding site. The interaction with sugars ...
Acetoacetate is an intermediate in the Krebs cycle which releases energy from sugars and carbohydrates.[22] ... Ozonolysis, and related dihydroxylation/oxidative sequences, cleave alkenes to give aldehydes or ketones, depending on alkene ... generated from carbohydrates, fatty acids, and amino acids in most vertebrates, including humans. Ketone bodies are elevated in ...
"Km+, a mannose-binding lectin from Artocarpus inegrifolia: amino acid sequence predicted tertiary structure, carbohydrate ...
... residue in the sequence Asn-X-Ser or Asn-X-Thr where X is any amino acid except proline. This sequence is called a ... The high sequence similarity between the prokaryotic and the eukaryotic STT3 suggests that their structures are similar. ...
"Callaway, Ewen (22 September 2011), "First Aboriginal genome sequenced", Nature, Nature News, doi:10.1038/news.2011.551 ... One hypothesis is that carbohydrate tubers (plant underground storage organs) may have been eaten in high amounts by pre- ... "Slowly digested and absorbed carbohydrate in traditional bushfoods: a protective factor against diabetes?". Am J Clin Nutr. 45 ...
Mahmood SN, Bowe WP (April 2014). "Diet and acne update: carbohydrates emerge as the main culprit". Journal of Drugs in ... scientists reported the first genome sequencing of a C. acnes bacteriophage (PA6). The authors proposed applying this research ... Eating fewer simple carbohydrates such as sugar may minimize the condition.[7] Treatments applied directly to the affected skin ... This led to the current understanding that acne could be explained by a sequence of related events, beginning with blockage of ...
carbohydrate binding. • identical protein binding. • receptor ligand activity. • extracellular matrix structural constituent. • ... A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and ... The nascent protein contains a N-terminal signal peptide sequence, a conserved "ZP domain" module, a consensus furin cleavage ...
8).[66] Upon heating of KC8, the elimination of potassium atoms results in the conversion in sequence to KC24, KC36, KC48 and ... carbohydrates and ionophore complexes.[218] ...
a b c d e Kai Müller, Thomas Borsch (2005): Phylogenetics of Amaranthaceae using matK/trnK sequence data - evidence from ... and specific root-located carbohydrates have been found in these plants.[4] ... Ivonne Sánchez del-Pino, Thomas Borsch, Timothy J. Motle (2009): trnL-F and rpl16 Sequence Data and Dense Taxon Sampling Reveal ... Clade and a Clarification of the Phylogenetic Position of Bienertia and Alexandra Using Multiple DNA Sequence Datasets. - ...
In 1977, Carl Woese, a microbiologist studying the genetic sequencing of organisms, developed a new sequencing method that ... Romano A; Conway T (1996). "Evolution of carbohydrate metabolic pathways". Res Microbiol. 147 (6-7): 448-55. doi:10.1016/0923- ... He sequenced a variety of different species and happened upon a group of methanogens that had vastly different patterns than ... Archaea were first classified as a separate group of prokaryotes in 1977 by Carl Woese and George E. Fox based on the sequences ...
Bioethanol is an alcohol made by fermentation, mostly from carbohydrates produced in sugar or starch crops such as corn, ...
McMullen BA, Fujikawa K, Kisiel W, et al. (1983). „Complete amino acid sequence of the light chain of human blood coagulation ... Sinha U, Wolf DL (1993). „Carbohydrate residues modulate the activation of coagulation factor X". J. Biol. Chem. 268 (5), 3048- ... The role of the carbohydrate moieties in the activation of factor X". Eur. J. Biochem. 218 (1), 153-63. o. DOI:10.1111/j.1432- ...
2001). "The sequence of the human genome". Science. 291 (5507): 1304-51. doi:10.1126/science.1058040. PMID 11181995.. ... "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A ...
The microbes are also responsible for metabolising the carbohydrates and amino acids present in the tea leaves.[15][16][17] ... For instance, the seven digit sequence 8653-602, would indicate the second production in 2006 of factory recipe 8653. Some ... It is apparent that this species does not have the gene sequence for coding ochratoxin and thus pu'er tea should be considered ...
The specific DNA sequence in the MCM6 gene helps control whether the LCT gene is turned on or off.[19] At least several ... Inborn error of carbohydrate metabolism: monosaccharide metabolism disorders Including glycogen storage diseases (GSD) ... "Diagnosing and Treating Intolerance to Carbohydrates in Children". Nutrients. 8 (3): 157. doi:10.3390/nu8030157. PMC 4808885 ...
Molecular Sequences and the Early History of Life. En: Sapp J, editor. "Microbial Phylogeny and Evolution: Concepts and ... Romano A, Conway T (1996). "Evolution of carbohydrate metabolic pathways". Res Microbiol 147 (6-7): 448-55. PMID 9084754. doi: ... "Shotgun sequencing finds nanoorganisms". UCBerkely News (en inglés). Descubrimento do grupo ARMAN de arqueas.. ... Lake JA (xaneiro de 1988). "Origin of the eukaryotic nucleus determined by rate-invariant analysis of rRNA sequences". Nature ...
The sequence of steps in which the reorganization of chemical bonds may be taking place in the course of a chemical reaction is ...
லைன் மற்றும் ரஷ் எழுதிய சிக்கலான காபோவைதரேட்டு மூலக்கூறு நோய்த்தடுப்பு (Molecular Immunology of Complex Carbohydrates) (A. Wu, ... இதில் 7 ஆவது குறியீடு செய்யப்பட்ட மரபணுக் கோர்வை மிகவும் பெரியதாகவும், பெரும்பாலான குறியாக்க வரிசை (coding sequence) களைக் ... in Molecular Immunology of Complex Carbohydrates (A. Wu, E. Kabat, Eds.) Plenum Publishing Corporation, N.Y. NY. ... Evidence that Band 3 Carbohydrate on Human Erythrocytes Carries the Majority of ABH Blood Group Substance" ...
"Nutrition for everyone: carbohydrates". Centers for Disease Control and Prevention, US Department of Health and Human Services ... It is necessary to consider that oats include many varieties, containing various amino acid sequences and showing different ... Oats are also commonly used as feed for horses when extra carbohydrates and the subsequent boost in energy are required. The ... protein amino acid sequences, and the immunoreactivities of toxic prolamins which vary among oat varieties.[3][4][22] Also, ...
Nectar is collected by foraging worker bees as a source of water and carbohydrates in the form of sucrose. The dominant ... inferred from nuclear and mitochondrial DNA sequence data"". Molecular Phylogenetics and Evolution. 40 (1): 315. doi:10.1016/j. ... inferred from nuclear and mitochondrial DNA sequence data". Molecular Phylogenetics and Evolution. 37 (1): 25-35. doi:10.1016/j ... has apparently increased the speed of its evolution and confounded the DNA sequence data to a point where little of substance ...
... new challenges in the era of oligogenism and next-generation sequencing". European Journal of Endocrinology. 178 (3): R55-R80. ... Carbohydrate metabolism: Glucose-6-phosphate dehydrogenase deficiency. *Pyruvate dehydrogenase deficiency. *Danon disease/ ...
... s are high in protein, complex carbohydrates, folate, and iron.[23] Beans also have significant amounts of fiber and ... "Mesoamerican origin of the common bean (Phaseolus vulgaris L.) is revealed by sequence data". Proceedings of the National ... These carbohydrates therefore leave the upper intestine unchanged and enter the lower reaches, where our resident bacterial ... This is because they contain large amounts of carbohydrates that human digestive enzymes can't convert into absorbable sugars. ...
Advances in Carbohydrate Chemistry and Biochemistry. 35. pp. 1-29. doi:10.1016/S0065-2318(08)60217-6. ISBN 9780120072354. . ... The vast majority of animals and plants are able to synthesize vitamin C, through a sequence of enzyme-driven steps, which ... Koshizaka T, Nishikimi M, Ozawa T, Yagi K (1988). "Isolation and sequence analysis of a complementary DNA encoding rat liver L- ... carbohydrates, sugars, and salt. Voluntary nutrients may be shown if present in significant amounts. Instead of Daily Values, ...
positive regulation of sequence-specific DNA binding transcription factor activity. • cellular response to nicotine. • positive ... trafficking is carbohydrate independent. In an effort to localize the amino acid motif responsible for granule targeting, we ... working with pre-sequencing era data, reasoned that excessive production of TNF causes malaria disease and endotoxin poisoning. ... constructed additional fusion proteins and analyzed their trafficking, concluding that granule-targeting sequences are ...
... protein and carbohydrates to regulate adherence bacteria to inhibit competitive bacteria). The enterococcal surface protein ( ... Genome sequencesEdit. The 22 sequenced Enterococcus faecium genomes:[9]. Strain. ST. CC17. Country. Year ...
DNA sequencing, a method developed by Walter Gilbert and Frederick Sanger in 1977,[5] caused a rapid change the development of ... The antigen (usually a protein or carbohydrate made by an infectious agent) is bound by the antibody, allowing this type of ... In 1995 a team at The Institute for Genomic Research sequenced the first bacterial genome; Haemophilus influenzae.[6] A few ... Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors" Proceedings of the National Academy of ...
... Genome Sequencing Project - Sequencing of the twelve tomato chromosomes.. *Tomato core collection database - Phenotypes ... A tomato is 95% water, contains 4% carbohydrates and less than 1% each of fat and protein (table). In a 100 gram amount, raw ... began sequencing the tomato genome in 2004, and is creating a database of genomic sequences and information on the tomato and ... "Tomato Genome Shotgun Sequence Prerelease".. *^ Sato, S.; Tabata, S.; Hirakawa, H.; Asamizu, E.; Shirasawa, K.; Isobe, S.; ...
carbohydrate metabolic process. • regulation of receptor activity. • negative regulation of transcription from RNA polymerase ... These three DNA sequences bind to CTCF in a way that limits downstream enhancer access to the Igf2 region. The mechanism in ...
A mannose sugar is added to the first tryptophan residue in the sequence W-X-X-W (W indicates tryptophan; X is any amino acid ... Glycosylation (see also chemical glycosylation) is the reaction in which a carbohydrate, i.e. a glycosyl donor, is attached to ... Glycosylation is the process by which a carbohydrate is covalently attached to a target macromolecule, typically proteins and ... GlycoEP : In silico Platform for Prediction of N-, O- and C-Glycosites in Eukaryotic Protein Sequences PLoS ONE 8(6): e67008 ...
"for his investigations on carbohydrates and vitamin C"[32] پاول کرر سویٹزرلینڈ "for his investigations on carotenoids, flavins ... "for his work on ribonuclease, especially concerning the connection between the amino acid sequence and the biologically active ... "for his discovery of sugar nucleotides and their role in the biosynthesis of carbohydrates"[62] ... "for their contributions concerning the determination of base sequences in nucleic acids"[72] ...
Does the amino acid sequence of the transferred proteins resemble the sequence of known allergenic proteins? Are the ...
An easy-to-use directory for life science and biomedical research products. Find special deals on products, order catalogs and browse product lines from suppliers of reagents, antibodies, laboratory equipment, and more.
... Seizi Someya,1 Masanori Kakuta,1 ... Carbohydrate-binding proteins are proteins that can interact with sugar chains but do not modify them. They are involved in ... Our method is based on support vector machines (SVMs). We first clarified the definition of carbohydrate-binding proteins and ... We also examined two amino acid grouping methods that enable effective learning of sequence patterns and evaluated the ...
The sequences of carbohydrate-binding protein sequences were collected from UniProtKB [9] by using a sequence retrieval system ... The sequence homology search was applied to these sequence clusters as follows.(1)For clusters consisting of a single sequence ... sequence the query sequence is predicted as a carbohydrate-binding (noncarbohydrate-binding) protein. The AUC value for this ... we regarded a protein sequence as that of a carbohydrate-binding protein (positive) if it had a sequence identity higher than ...
Scientific Experts about Experts and Doctors on carbohydrate sequence in Taiwan ... You are here: Locale , Taiwan , Experts and Doctors on carbohydrate sequence in Taiwan ... Wu A, Wu J, Tsai M, Kaltner H, Gabius H. Carbohydrate specificity of a galectin from chicken liver (CG-16). Biochem J. 2001;358 ... Yen M, Wu A, Yang Z, Gong Y, Chang E. Recognition roles of the carbohydrate glycotopes of human and bovine lactoferrins in ...
THE X-RAY CRYSTAL STRUCTURE OF PPACK-MEIZOTHROMBIN DESF1: KRINGLE/THROMBIN AND CARBOHYDRATE/KRINGLE/THROMBIN INTERACTIONS AND ... View Sequence & DSSP Image. Download Sequence Chain Image. Chain Info. Polymer: 1. Length: 159 residues. Chain Type: ... View Sequence & DSSP Image. Download Sequence Chain Image. Chain Info. Polymer: 2. Length: 259 residues. Chain Type: ... Sequence Display for the Entities in PDB 1A0H The graphical representation below shows this entrys sequences as reported in ...
LINUCS: LInear Notation for Unique description of Carbohydrate Sequences Introduction. LINUCS was chosen to fulfil to following ... LINUCS: linear notation for unique description of carbohydrate sequences.. Carbohydr Res 2001, 336: 1-11.. ... The first step is to transform the carbohydrate in SWEET-notation and the second step is to make it unique. ... Primary structure of the carbohydrate chain of soybean agglutinin. A reinvestigation by high resolution 1H NMR spectroscopy. J ...
Encoding schemes used in various carbohydrate sequence databases. The N-glycan core structure has been chosen to illustrate the ... Figure 2: Encoding schemes used in various carbohydrate sequence databases. The N-glycan core structure has been chosen to ... Figure 2: Encoding schemes used in various carbohydrate sequence databases. The N-glycan core structure has been chosen to ... Conclusion: GlycomeDB is a new, publicly available database for carbohydrate sequences with a unified, all-encompassing ...
Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing. 1st January ... Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing. ... demonstrating that this technique may prove useful for the sequencing of complex oligosaccharides. ...
Characterization of residues and sequences of the carbohydrate recognition domain required for cell surface localization and ... Characterization of residues and sequences of the carbohydrate recognition domain required for cell surface localization and ... Characterization of residues and sequences of the carbohydrate recognition domain required for cell surface localization and ... Characterization of residues and sequences of the carbohydrate recognition domain required for cell surface localization and ...
"Carbohydrate-binding sequences of the lectin genes in leguminous plants from the Galegeae and Hedysareae Tribes, Russian ... Carbohydrate-binding sequences of the lectin genes in leguminous plants from the Galegeae and Hedysareae Tribes. Carbohydrate- ... Carbohydrate-binding sequences of the lectin genes in leguminous plants from the Galegeae and Hedysareae Tribes. Chubukova, O. ... The carbohydrate-binding sequences (CBS) of the lectin genes from legume plants of the genera Astragalus Lam., Oxytropis DC., ...
Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2-40(T). ... Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2-40(T) ... The sequences were then biochemically characterized by a team led by Jennifer Doudnas group at UC Berkeley. Read more ... Cas9 needs guide RNA to lead it to the correct sequence to snip-but not all guides are effective. Researchers created a set of ...
Amino acid sequence similarities of carbohydrate active enzymes identified from the muskoxen rumen metatranscriptome (using all ... pone-0020521-g005: Amino acid sequence similarities of carbohydrate active enzymes identified from the muskoxen rumen ... pone-0020521-g005: Amino acid sequence similarities of carbohydrate active enzymes identified from the muskoxen rumen ... Polyadenylated RNA (mRNA) was sequenced on the Illumina Genome Analyzer II system and 2.8 gigabases of sequences were obtained ...
Glycolysis - how do carbohydrates enter the glycolytic sequence? (Replies: 1) * Biology test questions - How are my answers ( ... Total carbohydrates is composed of two parts fibers and sugar and some unspecified carbohydrates so that means 24 g = 4g fiber ... So the question says calories from a carbohydrate which is sugar alone hence 12/24 = 50% since only total carbohydrates ... Re: Test question: How many carbohydrates in this food were contribut. I am sorry, but I am still confused as to how you got ...
Little is known about the microbial diversity and its effect on carbohydrate metabolism in ripe fruits. In this study, we aimed ... In this study, we aimed to understand the diversity and function of microorganisms in relation to carbohydrate metabolism of ... Results of this study underline the links between the host and fruit-associated microbiome in carbohydrate metabolism of the ... to understand the diversity and function of microorganisms in relation to carbohydrate metabolism of ripe watermelon fruits. We ...
DNA sequencing. Genomic DNA was prepared from various alleles of l(2R)W5 in trans to another l(2R)W chromosome using Puregene ( ... bnch is required for oogenesis and carbohydrate mobilization from yolk spheres. (A and B) Germline clones of bnchE14.1 give ... Aberrant lysosomal carbohydrate storage accompanies endocytic defects and neurodegeneration in Drosophila benchwarmer. Bart ... Here, we report that loss of Drosophila benchwarmer (bnch), a predicted lysosomal sugar carrier, leads to carbohydrate storage ...
Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These ... Sequence Length. Organism. Details. Image. MACROPHAGE MANNOSE RECEPTOR. A, B. 147. Homo sapiens. Mutation(s): 0 Gene Names: ... Structure of a C-type carbohydrate recognition domain from the macrophage mannose receptor.. Feinberg, H., Park-Snyder, S., ... Of the two Ca(2+) sites, only the principal site known to mediate carbohydrate binding in other C-type lectins is occupied. ...
0076]Carbohydrate utilization-related nucleotide sequences identified based on their sequence identity to the carbohydrate ... As used herein, "5 leader sequence," "translation leader sequence," or "5 non-coding sequence" refer to that DNA sequence ... sequence identity to the corresponding sequence may be used. [0098]Given the coding-strand sequence encoding a carbohydrate ... Sequence Identity [0064]The carbohydrate utilization-related and multidrug transporter sequences are members of families of ...
Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing. October 13, ... demonstrating that this technique may prove useful for the sequencing of complex oligosaccharides. ...
Which sequence most accurately describes antibody-mediated immunity? 1. B cell divides and gives rise to clone .... Biology ( ... Both carbohydrates and protein have 4 calories per gram. T F. Nutrition ...
The carbohydrate active enzymes (CAZymes) constitute the majority of the secretory proteins. The present study is the maiden ... The genome sequences of A. elegans and A. trapeziformis are available in public database, but not A. variabilis. We, therefore ... The whole genome of A. variabilis NCCPF 102052 was sequenced and the genomic structure of A. variabilis was compared with ... Together with available genome sequence of A. elegans and A. trapeziformis, the study helped to indicate the possible virulence ...
... carbohydrate fermentation, reactivity to LcS-specific antibody, and the cytokine-inducing ability of LcS in the flight sample ... Profiles of randomly amplified polymorphic DNA, sequence variant frequency, ... Profiles of randomly amplified polymorphic DNA, sequence variant frequency, carbohydrate fermentation, reactivity to LcS- ... Table 2 Results of carbohydrate fermentation testing of the flight sample (FS) and the US and Japanese ground controls (GC-US ...
... and this deficit is compensated for by the myriad of carbohydrate-active enzymes (CAZymes) encoded by members of the gut ... He concentrates on the sequence-structure-specificity relationships of carbohydrate-active enzymes to understand the ... Nature 490, 55-60 (2012). This huge metagenomic sequencing effort on the gut microbiota of 124 individuals has revealed a set ... Nature 457, 480-484 (2009). Metagenomic and 16S rRNA sequencing suggest the existence of a functional core microbiome and that ...
... is shown to mediate cell adhesion by binding a sialylated carbohydrate structure, sialyl-Lewis x, found on neutrophils, ... Carbohydrate Conformation * Carbohydrate Sequence * Cell Adhesion Molecules / immunology* * Cell Adhesion* * Cell Line ... CD62 and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1) recognize the same carbohydrate ligand, sialyl-Lewis x Proc ... is shown to mediate cell adhesion by binding a sialylated carbohydrate structure, sialyl-Lewis x, found on neutrophils, ...
Occurrence of N-acetylglucosamine 6-phosphate in complex carbohydrates. Characterization of a phosphorylated sialyl ... the first evidence for the occurrence of N-acetylglucosamine 6-phosphate as an integral component in complex carbohydrates. ... Carbohydrate Sequence * Cattle * Chromatography, Thin Layer * Colostrum / analysis* * Escherichia coli / enzymology * Female ...
Inferred from Sequence Orthology. more info. carbohydrate metabolic process IEA Inferred from Electronic Annotation. more info ... These reference sequences exist independently of genome builds. Explain. These reference sequences are curated independently of ... Metabolism of carbohydrates, organism-specific biosystem (from REACTOME) Metabolism of carbohydrates, organism-specific ... Source sequence(s). AK005237, BC050940, BY586895, CA559418 Consensus CDS. CCDS24466.1 UniProtKB/Swiss-Prot. P14152 Related. ...
Congruent Strategies for Carbohydrate Sequencing. 3. OSCAR: An Algorithm for Assigning Oligosaccharide Topology from MS n Data ...
... partial sequences of 25 candidate genes were characterized for polymorphisms using a panel of 92 diverse pea lines. Variation ... The association of polymorphisms in carbohydrate and starch metabolic genes with variation in amylopectin chain length ... chain elongation and branching stages of the pea carbohydrate and starch metabolic pathways. ... Polymorphisms (SNPs and indels) in sequences from 25 starch and carbohydrate metabolic genes were extracted from sequence ...
It is also used for confirmation of disulfide bonds, location of carbohydrate attachment, sequence analysis, and for ... Protein Sequencing - (See Amino Acid Sequencing).. Rabbit Pyrogen Test. U.S.P. - An assay for the presence of pyrogens (not ... Amino Acid Sequencing - A partial sequencing (8- 15 residues) of amino acids within a protein or polypeptide by either amino- ... Full sequence is often done by sequencing the peptide fragments isolated from HPLC fractionation. ...
Genome sequencing information. Genome project history. Bosea sp. WAO was selected for sequencing based on the organisms ... Genome sequencing and assembly. A paired-end library was constructed using an Illumina Nextera Kit and sequenced using an ... Ten other genome sequences of Bosea spp. are publicly available of which four are validly named and characterized to the ... The minimum information about a genome sequence (MIGS) specification. Nat Biotechnol. 2008;26:541-7.CrossRefPubMedPubMedCentral ...
54 ENVIRONMENTAL SCIENCES; carbohydrate metabolism; sulfur; ribosomal RNA; sequence assembly tools; metagenomics; gene mapping ... Thus, the metabolic profile revealed a relative increase in genes responsible for carbohydrate metabolism, respiration, ... in hydraulic fracturing source water and produced water from natural gas extraction characterized by metagenomic sequencing ... in hydraulic fracturing source water and produced water from natural gas extraction characterized by metagenomic sequencing ...
  • Little is known about the microbial diversity and its effect on carbohydrate metabolism in ripe fruits. (frontiersin.org)
  • In this study, we aimed to understand the diversity and function of microorganisms in relation to carbohydrate metabolism of ripe watermelon fruits. (frontiersin.org)
  • Results of this study underline the links between the host and fruit-associated microbiome in carbohydrate metabolism of the ripe fruits. (frontiersin.org)
  • Thus, the metabolic profile revealed a relative increase in genes responsible for carbohydrate metabolism, respiration, sporulation and dormancy, iron acquisition and metabolism, stress response and sulfur metabolism in the produced water samples. (osti.gov)
  • A necessary step for a genome level analysis of the cellular metabolism is the in silico reconstruction of the metabolic network from genome sequences. (biomedcentral.com)
  • Carbohydrate-binding proteins are proteins that can interact with sugar chains but do not modify them. (hindawi.com)
  • We first clarified the definition of carbohydrate-binding proteins and then constructed positive and negative datasets with which the SVMs were trained. (hindawi.com)
  • Sugar chains and carbohydrate-binding proteins play important roles in several biological processes such as cell-to-cell signaling, protein folding, subcellular localization, ligand recognition, and developmental processes [ 1 ]. (hindawi.com)
  • With the rapid increase in the amount of available glycoprotein data (i.e., protein sequences), there is a growing interest in the functions, physicochemical properties, and tertiary structures of carbohydrate-binding proteins and in their applications. (hindawi.com)
  • Experimental work to identify carbohydrate-binding proteins is costly and time consuming, so computational methods to predict carbohydrate-binding proteins would be useful. (hindawi.com)
  • Carbohydrate-binding proteins are nonantibody proteins that can interact with sugar chains, and various keywords are used to annotate them in biological databases: "carbohydrate-binding protein", "lectin", and so on. (hindawi.com)
  • Furthermore, heparin-binding proteins and hyaluronic-acid-binding proteins are also carbohydrate-binding proteins, but are not usually annotated "carbohydrate-binding protein" or "lectin. (hindawi.com)
  • In the work reported in this paper, we first collected carbohydrate-binding proteins of various kinds, including enzymes and proteins not explicitly annotated as "carbohydrate-binding protein," and specified a set of search conditions for carbohydrate-binding proteins in the amino acid sequence database UniProt Knowledgebase (UniProtKB). (hindawi.com)
  • Based on the collected proteins, we developed a carbohydrate-binding protein prediction system by using machine learning methods, with which predicting carbohydrate-binding proteins can be formulated as a binary classification problem. (hindawi.com)
  • To the best of our knowledge, however, the only reported methods for predicting carbohydrate-binding proteins are conventional homology-based methods. (hindawi.com)
  • Although carbohydrates are the third major class of biological macromolecules, after proteins and DNA, there is neither a comprehensive database for carbohydrate structures nor an established universal structure encoding scheme for computational purposes. (nih.gov)
  • This fact probably points to the differences in the carbohydrate-binding specificity of the proteins examined, which can eventually affect their functional activity. (deepdyve.com)
  • In addition, carbohydrate utilization-related and multidrug transporter fusion proteins, antigenic peptides, and anti-carbohydrate utilization-related and anti-multidrug transporter antibodies are encompassed. (patentsencyclopedia.com)
  • The carbohydrate active enzymes (CAZymes) constitute the majority of the secretory proteins. (biomedcentral.com)
  • I have been trying to get my head around the basics for these four (Carbohydrates, Proteins, Amino Acids and Lipids) for about a week and I just don't understand the basic structure, formulas and such. (physicsforums.com)
  • The complete or partial sequence of four different bacteriophage genomes is also present, with each containing genes for one or more previously undiscovered superantigen-like proteins. (pnas.org)
  • However, the fimbrial nature of the sialic acid binding property and sequence information about FimA proteins from multiple strains are lacking. (biomedcentral.com)
  • Three unique subtypes of FimA proteins with 63.8-66.4% sequence identity were present in strains of A. naeslundii genospecies 1 and 2 and A. odontolyticus . (biomedcentral.com)
  • Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties. (uniprot.org)
  • Sequencing and analysis of its genome revealed a single circular chromosome of 3,402,093 bp encoding 3,143 predicted proteins. (asm.org)
  • The mobilities of the variant transmembrane proteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis suggested that all four sites are utilized for carbohydrate attachment. (asm.org)
  • The present invention further provide methods of producing proteins and other products of interest and methods of controlling expression of nucleic acid sequences of interest using the seed specific promoter regions. (osti.gov)
  • Glycans, proteins to which they are attached (glycoproteins) and proteins to which they bind (lectins or carbohydrate-binding proteins) are the main molecular actors in this overall cell surface picture as well as the enzymes that are needed to synthesize or trim the attached glycans. (mcponline.org)
  • Amino acid sequence similarities of carbohydrate active enzymes identified from the muskoxen rumen metatranscriptome (using all assembled contigs) versus top BLASTX match to the Genbank nr database. (nih.gov)
  • Carbohydrate binding domain (CBM) family 10/fungal dockerin domains were predominant in these multi domain enzymes, and were found in 190 (78.5%) of these contigs. (nih.gov)
  • Abdessamad El Kaoutari is preparing his Ph.D. at the Medical School of Aix-Marseille University, Marseille, France, on the determination of the carbohydrate-active enzymes profile of the human gut microbiota. (nature.com)
  • He concentrates on the sequence-structure-specificity relationships of carbohydrate-active enzymes to understand the glycobiological content of genomes and metagenomes. (nature.com)
  • Among the myriad of genes that have been identified in the human gut microbiome, those that encode carbohydrate-active enzymes (CAZymes) are of particular interest, as these enzymes are required to digest most of our complex repertoire of dietary polysaccharides. (nature.com)
  • A model human microbiome was constructed from 177 microbial genomes in proportions that approximate their representation in the healthy adult gut, and this mini-microbiome was used to evaluate the diversity of carbohydrate-active enzymes (CAZymes) in the gut microbiota. (nature.com)
  • The Geobacillus strains rely on a small number of secreted enzymes located within distinct clusters for carbohydrate utilization, in contrast to most biomass-degrading organisms which contain numerous secreted enzymes located randomly throughout the genomes. (frontiersin.org)
  • Bins exhibited differences in their degree of sequence variation, gene content including the repertoire of carbohydrate active enzymes present within and between twins (e.g., predicted cellulases, dockerins), and transcriptional activities. (pnas.org)
  • In higher organisms glycoside hydrolases are found within the endoplasmic reticulum and Golgi apparatus where they are involved in processing of N-linked glycoproteins , and in the lysosome as enzymes involved in the degradation of carbohydrate structures. (wikipedia.org)
  • Sequence-based classifications are among the most powerful predictive method for suggesting function for newly sequenced enzymes for which function has not been biochemically demonstrated. (wikipedia.org)
  • The online database is supported by CAZypedia, an online encyclopedia of carbohydrate active enzymes. (wikipedia.org)
  • The carbohydrates that are inside the glycoprotein are modified by enzymes that also takes away some of the sugars. (reference.com)
  • GLYCOSCIENCES.de, the Consortium for Functional Glycomics (CFG), the Kyoto Encyclopedia of Genes and Genomes (KEGG) and the Bacterial Carbohydrate Structure Database (BCSDB). (nih.gov)
  • RT "Sequencing the genomes of 1000 actinobacteria strains. (univ-lyon1.fr)
  • Over the past several years, selected regions of the japonica and indica rice genomes have been sequenced. (sciencemag.org)
  • Similar high-accuracy sequencing projects of important cereals would be expensive and slow because their genomes are so large. (sciencemag.org)
  • Recent improvements in automated DNA sequencing have made whole-genome shotgun sequencing an attractive approach for gene discovery in both small and large genomes ( 16-18 ). (sciencemag.org)
  • Muldashev, A. 2014-05-30 00:00:00 The carbohydrate-binding sequences (CBS) of the lectin genes from legume plants of the genera Astragalus Lam. (deepdyve.com)
  • We, therefore, performed the whole genome sequence of A. variabilis to explore its genomic structure and possible genes determining the virulence of the organism. (biomedcentral.com)
  • To understand how allelic variation in the pea starch metabolic pathway affects starch structure and percent amylose, partial sequences of 25 candidate genes were characterized for polymorphisms using a panel of 92 diverse pea lines. (biomedcentral.com)
  • The genes with associated polymorphisms are involved in the substrate supply, chain elongation and branching stages of the pea carbohydrate and starch metabolic pathways. (biomedcentral.com)
  • The association of polymorphisms in carbohydrate and starch metabolic genes with variation in amylopectin chain length distribution and percent amylose may help to guide manipulation of pea seed starch structural and functional properties through plant breeding. (biomedcentral.com)
  • The 1,852,442-bp sequence of an M1 strain of Streptococcus pyogenes , a Gram-positive pathogen, has been determined and contains 1,752 predicted protein-encoding genes. (pnas.org)
  • Frame-shift and point mutations were corrected when appropriate, with ORFs containing sequence-verified frameshift or point-verified mutations designated as putatively inactive genes. (pnas.org)
  • Here we have sequenced fimA genes from strains of A.naeslundii genospecies 1 (n = 4) and genospecies 2 (n = 4), both of which harboured variant Galβ-dependent hemagglutination (HA) types, and from A.odontolyticus PK984 with a sialic acid-dependent HA pattern. (biomedcentral.com)
  • The major subunit genes of type-2 and type-1 fimbriae, fimA and fimP , respectively, have been cloned and sequenced from A. naeslundii genospecies 1 (strain 12104) and 2 (strain T14V) [ 22 - 26 ]. (biomedcentral.com)
  • Because of limitations imposed by sequencing costs and throughput, these studies have examined the more abundant species or genes. (pnas.org)
  • Moreover, how much of the observed organismal diversity is an artifact of noise introduced during PCR and sequencing of 16S rRNA genes ( 1 - 3 )? (pnas.org)
  • A 454 pyrosequencing method was used to obtain 1.2-1.5 million sequencing reads from PCR-amplified V2 regions of bacterial 16S rRNA genes present in each fecal sample (average read length ∼232 nt), and 3.8-6.3 Gbp of single- and paired-end shotgun reads from total fecal community DNA ( Table S1 ). (pnas.org)
  • Using a method for rRNA depletion based on a combination of size selection (to remove 5S rRNA and tRNA), and streptavidin bead-based pull-down of biotinylated oligonucleotides hybridized to domains conserved among gut bacterial rRNA genes ( 5 ), we enriched for fecal mRNA and then generated 12-16 million sequencing reads representing expressed genes in their microbiomes ( Table S2 ). (pnas.org)
  • Gene predictions on the assembled sequence suggest that the genome contains 32,000 to 50,000 genes. (sciencemag.org)
  • The inability of N. winogradskyi to grow on C 6 molecules is consistent with the genome sequence, which lacks genes for complete Embden-Meyerhof and Entner-Doudoroff pathways, and active uptake of sugars. (asm.org)
  • These libraries come in handy as a starting point for cloning eukaryotic genes, since all the coding sequences are given. (gradesaver.com)
  • Instead of using predicted genes or CDSs to query public databases, entries from public DNA or protein databases are used as queries to search a local database of the unannotated genome sequence to predict CDSs. (biomedcentral.com)
  • Computer-assisted analysis of nucleotide and predicted amino acid sequences of the lectin gene fragments examined revealed a high homology level between their CBS. (deepdyve.com)
  • This gene encodes the carbohydrate-binding protein malectin which is a Type I membrane-anchored endoplasmic reticulum protein. (nih.gov)
  • Substantial insight into the patterns of variation in the microbiota between body habitats and individuals has been gained using shallow sequencing of 16S rRNA gene amplicons and community DNA. (pnas.org)
  • The small genome and predicted high gene density of rice make it an attractive target for cereal gene discovery efforts and genome sequence analysis. (sciencemag.org)
  • In addition, expressed gene sequencing has been actively pursued. (sciencemag.org)
  • The gene was successfully overexpressed in Pichia pastoris , and the recombinant protein was designated carbohydrate-binding cytochrome b 562 (CBCyt. (asm.org)
  • RT "Identification of the characteristic amino-acid sequence for human RT alpha-amylase encoded by the AMY2B gene. (genome.jp)
  • CHST10 (Carbohydrate Sulfotransferase 10) is a Protein Coding gene. (genecards.org)
  • Clinical finding Evaluation finding Genetic finding Gene expression Genetic polymorphism Molecular sequence data and a host of other genetic terms for states, processes, abnormalities, etc. (w3.org)
  • Genomic comparison of the 12 gene group A carbohydrate synthetic cluster gacA-L ( 1 ) from S. pyogenes with the corresponding regions of the 35 GAS/ST128/SE revealed an upstream crossover point within the S. pyogenes gacE ABC transporter gene and a downstream crossover point within ebsA ( Figure 2 ). (cdc.gov)
  • We also found these same crossover points within the group carbohydrate gene cluster of the available genomic sequence from the previously described invasive GAS/SE strain AC-2713 recovered in 1999 ( 3 ). (cdc.gov)
  • Compared to other gene finding methods such as CRITICA our method is more suitable for exploiting sequences of low genome coverage. (biomedcentral.com)
  • Wu A, Wu J, Tsai M, Kaltner H, Gabius H. Carbohydrate specificity of a galectin from chicken liver (CG-16). (labome.org)
  • These data show that the carbohydrate plays no direct role in determining the antigenic specificity of HLA antigens. (elsevier.com)
  • 97.2%) within a genospecies revealed species specific sequences or segments that coincided with binding specificity. (biomedcentral.com)
  • RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs. (genome.jp)
  • Isolates collected from a population of ≈34 million persons are subjected to whole-genome sequence (WGS)-based characterization. (cdc.gov)
  • These sequences also showed homology with variable regions of human immunoglobulin heavy chains and with NH 2 -terminal sequences of HLA heavy chains. (elsevier.com)
  • Perhaps what impressed me most (and the reason I keep referring it to others) was that it contains the most accessible description of sequence homology searches that I have ever read, giving practical advice on how to get the most out of BLAST and related homology search tools. (google.com.mx)
  • Sequence analysis of the cDNA clone obtained by PCR confirmed this homology. (portlandpress.com)
  • Homology alignment reveals that the amino acid sequences contain some conserved function domains. (mdpi.com)
  • For each database, different encoding schemes for residues and sequence topology were designed. (nih.gov)
  • We also investigated the sequences and residues in CRD that were essential for protein cell surface localization and ligand binding. (biologists.org)
  • The C-terminal sequence (KANLRAQ) was also essential for protein folding and transport, while the four final residues (LRAQ) were involved in maintaining receptor function. (biologists.org)
  • The carbohydrate moiety of HLA antigens was radioactively labeled in galactose residues by treatment of membranes with neuraminidase, galactose oxidase, and NaB 3 H 4 . (elsevier.com)
  • Removal by glycosidases of all the carbohydrate, except for single residues of N-acetylglucosamine and fucose, from the intact molecule was accompanied by no change in HLA antigenic activity. (elsevier.com)
  • [4] [8] The database provides a series of regularly updated sequence based classification that allow reliable prediction of mechanism (retaining/inverting), active site residues and possible substrates. (wikipedia.org)
  • Instead, carbohydrates that contain many different types of sugar residues attach to the translated protein. (reference.com)
  • The graphical representation below shows this entry's sequences as reported in UniProtKB, in the sample (SEQRES), or as observed in the experiment (ATOM). (rcsb.org)
  • Taxon clustering based on similarities of glycomes (carbohydrate-based tree of life) Glycosyltransferase subdatabase (GT-explorer) Until 2015, Bacterial Carbohydrate Structure Database (BCSDB) and Plant&Fungal Carbohydrate Structure Database (PFCSDB) databases existed in parallel. (wikipedia.org)
  • [9] Based on three-dimensional structural similarities, the sequence-based families have been classified into 'clans' of related structure. (wikipedia.org)
  • In this report, we present the complete genomic sequence of a class I strain of S. pyogenes . (pnas.org)
  • The available methods often encounter difficulties when dealing with unfinished error-containing genomic sequence. (biomedcentral.com)
  • Other rice genome sequencing projects have been reported by Monsanto Co. ( 10 ) and by the Beijing Genomics Institute ( 11 ). (sciencemag.org)
  • We have identified a ~200 kb unique super-cluster in all six strains, containing five to eight distinct carbohydrate degradation clusters in a single genomic region, a feature not seen in other genera. (frontiersin.org)
  • Input of extended, non-graphic nomenclature to describe carbohydrate structures. (glycosciences.de)
  • The whole genome of A. variabilis NCCPF 102052 was sequenced and the genomic structure of A. variabilis was compared with already available genome structures of A. elegans, A. trapeziformis and other medically important Mucorales . (biomedcentral.com)
  • The main data stored in CSDB are carbohydrate structures of bacterial, fungal, and plant origin. (wikipedia.org)
  • As of 2017, the coverage on bacteria and archaea is ca. 80% of carbohydrate structures published in scientific literature The time lag between the publication of relative data and their deposition into CSDB is about 18 months. (wikipedia.org)
  • The synthesizer allows us to make not one but many carbohydrate structures from a particular organism and test those to see if they protect against the microbe. (phys.org)
  • The import of this is found when one considers that if the amino acids were ordered in a different sequence, they would not have the same function. (reference.com)
  • RT "The DNA sequence and biological annotation of human chromosome 1. (genome.jp)
  • The available methods are mainly based on the annotation of genome sequences including two successive steps, the prediction of coding sequences (CDS) and their function assignment. (biomedcentral.com)
  • Polyadenylated RNA (mRNA) was sequenced on the Illumina Genome Analyzer II system and 2.8 gigabases of sequences were obtained and 59129 contigs assembled. (nih.gov)
  • Yen M, Wu A, Yang Z, Gong Y, Chang E. Recognition roles of the carbohydrate glycotopes of human and bovine lactoferrins in lectin-N-glycan interactions. (labome.org)
  • Our results showed that the carbohydrate recognition domain (CRD) was the ligand-binding domain of human LOX-1. (biologists.org)
  • The results demonstrate that although the two LEC-CAMs differ in their biological activities by their distribution and mode of expression, they are capable of mediating cell adhesion by recognition of the same carbohydrate ligand. (nih.gov)
  • Participates in biosynthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. (genecards.org)
  • The viral variants described in this report should prove useful for investigation of the contribution of carbohydrate moieties on gp41 to recognition by antibodies, shielding from antibody-mediated neutralization, and structure-function relationships. (asm.org)
  • He investigates the diversity and the metabolic pathways of the human gut microbiome in healthy and diseased states using next-generation sequencing data. (nature.com)
  • Complete genome sequence and metabolic potential of the quinaldine-degrading bacterium Arthrobacter sp. (biomedcentral.com)
  • Carbohydrate polymers are another metabolic niche of both A . aurescens TC1 and Arthrobacter sp. (biomedcentral.com)
  • In this work a fast method is proposed to use unannotated genome sequence for predicting CDSs and for an in silico reconstruction of metabolic networks. (biomedcentral.com)
  • The reversed querying process and the program IdentiCS allow a fast and adequate prediction protein coding sequences and reconstruction of the potential metabolic network from low coverage genome sequences of bacteria. (biomedcentral.com)
  • A comprehensive understanding of the metabolic network at the system level is particularly important for both biotechnological and biomedical research and is now made possible by rapid advances in genome sequencing and functional genomics. (biomedcentral.com)
  • RT "Complete genome sequence of the erythromycin-producing bacterium RT Saccharopolyspora erythraea NRRL23338. (univ-lyon1.fr)
  • Furthermore, two versions of sequences of the bacterium Klebsiella pneumoniae with different genome coverage (3.9 and 7.9 fold, respectively) are examined. (biomedcentral.com)
  • Secondary outcome measures include change in 2-hour postprandial serum concentrations of insulin, change in 4-hour postprandial serum concentrations of triacylglycerols, and change in fecal microbiota composition measured by 16S sequencing. (clinicaltrials.gov)
  • Draft genome sequence of Bosea sp. (springer.com)
  • Here we summarize the physiological features together with the draft genome sequence and data analysis of Bosea sp. (springer.com)
  • RT "Draft Genome Sequence of Clostridium straminisolvens Strain JCM RT 21531T, Isolated from a Cellulose-Degrading Bacterial Community. (genome.jp)
  • Acid pH-induced removal of this Ca(2+) results in conformational rearrangements of the receptor, rendering it unable to bind carbohydrate ligands. (rcsb.org)
  • In this work we report the whole genome sequences of six new Geobacillus xylanolytic strains along with the genomic analysis of their capability to degrade carbohydrates. (frontiersin.org)
  • Glycoside hydrolases are found in the intestinal tract and in saliva where they degrade complex carbohydrates such as lactose , starch , sucrose and trehalose . (wikipedia.org)
  • Covered in-depth are the mass spectrometry of carbohydrates, peptide sequencing by mass spectrometry, mass spectrometry of nucleic acid components, and mass spectrometry in pharmacology. (wiley.com)
  • Descriptions of the microbial communities that live on and in the human body have progressed at a spectacular rate over the past 5 years, fuelled primarily by highly parallel DNA-sequencing technologies and associated advances in bioinformatics, and by the expectation that understanding how to manipulate the structure and functions of our microbiota will allow us to affect health and prevent or treat diseases. (nature.com)
  • In this Analysis article, we examine the carbohydrate-digestive capacity of a simplified but representative mini-microbiome in order to highlight the abundance and variety of bacterial CAZymes that are represented in the human gut microbiota. (nature.com)
  • The immune system recognizes these carbohydrates as foreign material, and creates antibodies that launch an immune response to battle the infection. (phys.org)
  • You create antibodies against the sugar structure, and these antibodies block the carbohydrate toxin from leading to inflammation and anemia, the hallmarks of malarial infection," says Seeberger. (phys.org)
  • To establish the relationship among the amino acid sequences of the combining sites of plant lectins and mammalian glycotopes should be an important direction to be addressed in lectinology. (labome.org)
  • Cloning and sequence analysis of cDNAs encoding mammalian cytosolic malate dehydrogenase. (nih.gov)
  • Comparison of the amino acid sequences of mammalian and bacterial malate dehydrogenase. (nih.gov)
  • A genomic library is a collection of DNA fragments from a single organism that contains at least one copy of DNA sequence in the genome with the smallest possible number of duplicates. (gradesaver.com)
  • CSDB stores published structural, taxonomical, bibliographic and NMR-spectroscopic data on natural carbohydrates and carbohydrate-related molecules. (wikipedia.org)
  • Glycoscience is the science and technology of carbohydrates, which are the most abundant biological molecules on Earth and make up part of the biology of all living organisms. (coursera.org)
  • Speaking at the 237th National Meeting of the American Chemical Society, Peter H. Seeberger, Ph.D., described development of an automated carbohydrate synthesizer , a device that builds these intricate molecules in a few hours - rather than the months or years required with existing technology. (phys.org)
  • Developed at the Swiss Federal Institute of Technology in Zurich, the instrument produces significant quantities of carbohydrate molecules that were nearly inaccessible until now. (phys.org)
  • Carbohydrates are tough molecules to build because of their complicated, branched structure. (phys.org)
  • So instead of trying to build carbohydrates from scratch, scientists today use molecules isolated from nature, a painstaking process that could take months. (phys.org)
  • Of the two Ca(2+) sites, only the principal site known to mediate carbohydrate binding in other C-type lectins is occupied. (rcsb.org)
  • Plant cell wall degrading enzyme modules including glycoside hydrolases, carbohydrate esterases and polysaccharide lyases were identified from over 2500 contigs. (nih.gov)
  • Glycoside hydrolases may also be classified by sequence or structure based methods. (wikipedia.org)
  • Recent progress in glycosidase sequence analysis and 3D structure comparison has allowed the proposal of an extended hierarchical classification of the glycoside hydrolases. (wikipedia.org)
  • equisimilis (SE) isolates employing the gacI ( 1 ) sequence query within our bioinformatics pipeline ( 2 ). (cdc.gov)
  • Finally, ion-mobility mass spectrometry following fragmentation was used to determine the nature of both the reducing and non-reducing glycans of a series of epimeric disaccharides and the branched pentasaccharide Man3 glycan, demonstrating that this technique may prove useful for the sequencing of complex oligosaccharides. (jic.ac.uk)
  • The carbohydrate composition of pulses includes starch (22-45%), dietary fiber (15-32%) and oligosaccharides (α-galactosides). (biomedcentral.com)
  • The method involves examining the human tissue for the presence of a glycolipid antigen having the terminal carbohydrate sequence: GalNAcβl➝4Galβl➝3GalNAcβl➝4Galβl. (freepatentsonline.com)
  • Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. (uniprot.org)
  • Some of these N-linked glycoproteins contain carbohydrates. (reference.com)
  • Sequence & Structure Relationships. (rcsb.org)
  • Primary structure of the carbohydrate chain of soybean agglutinin. (glycosciences.de)
  • This is the same point where nature starts building the complex carbohydrate structure. (glycosciences.de)
  • GlycomeDB - integration of open-access carbohydrate structure databases. (nih.gov)
  • Funding for further development of the Complex Carbohydrate Structure Database (CCSD or CarbBank) ceased in 1997, and since then several initiatives have developed independent databases with partially overlapping foci. (nih.gov)
  • GlycomeDB is a new, publicly available database for carbohydrate sequences with a unified, all-encompassing structure encoding format and NCBI taxonomic referencing. (nih.gov)
  • The N-glycan core structure has been chosen to illustrate the different encoding schemes used in the various databases for a given carbohydrate sequence. (nih.gov)
  • The present study is the maiden attempt to sequence and analyze the genomic structure of A. variabilis . (biomedcentral.com)
  • The leukocyte receptor CD62, which is expressed on activated platelets and endothelial cells, is shown to mediate cell adhesion by binding a sialylated carbohydrate structure, sialyl-Lewis x, found on neutrophils, monocytes, and tumor cells. (nih.gov)
  • Structural studies including monosaccharide and phosphate analysis, glycosidase and phosphatase treatments, methylation analysis, and periodate treatment indicated the structure of this compound to be NeuAc alpha 2-6Gal beta 1-4GlcNAc-6-P. This provides the first evidence for the occurrence of N-acetylglucosamine 6-phosphate as an integral component in complex carbohydrates. (nih.gov)
  • If you do carbohydrates, similarly look at their biosynthesis at the same time as their structure. (physicsforums.com)
  • The deduced amino acid sequence implies that there is a two-domain structure consisting of an N-terminal cytochrome domain and a C-terminal family 1 carbohydrate-binding module (CBM1) but that the flavin-containing domain of CDH is not present. (asm.org)
  • Carbohydrate Structure Database (CSDB) is a free curated database and service platform in glycoinformatics, launched in 2005 by a group of Russian scientists from N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences. (wikipedia.org)
  • In 2015, they were joined into the single Carbohydrate Structure Database (CSDB). (wikipedia.org)
  • CSDB web site CSDB usage examples CSDB technical documentation CSDB Linear (structure encoding notation) Carbohydrate databases registered in NAR collection Carbohydrate databases in the recent decade (lection) Toukach Ph.V. (wikipedia.org)
  • and analysis and structure of cell membrane carbohydrates. (elsevier.com)
  • Synthesis of carbohydrate derivatives using solid-phase work-up and scavenging techniques. (biomedsearch.com)
  • Mass Spectrometry of Carbohydrates (C. Hellerqvist & B. Sweetman). (wiley.com)
  • Peptide Sequencing by Mass Spectrometry (J. Stults). (wiley.com)
  • More than 100000 datasets were imported, resulting in more than 33000 unique sequences now encoded in GlycomeDB using the universal format GlycoCT. (nih.gov)
  • However there is widespread interest in high-fat ("Atkins Diet") and high-protein ("Zone Diet") alternatives to the conventional high-carbohydrate, high-fibre approach. (springer.com)
  • 27 kg/m 2 ) were randomised to one of three dietary interventions: a high-carbohydrate, high-fibre (HC) diet, the high-fat (HF) Atkins Diet, or the high-protein (HP) Zone Diet. (springer.com)
  • The S. pyogenes genome sequence was determined by using the whole-genome shotgun approach. (pnas.org)
  • The genome of the japonica subspecies of rice, an important cereal and model monocot, was sequenced and assembled by whole-genome shotgun sequencing. (sciencemag.org)
  • CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. (genome.jp)
  • The expansion of hypervirulent sequence type 4821 clonal complex (CC4821) lineage Neisseria meningitidis bacteria has led to a shift in meningococcal disease epidemiology in China, from serogroup A (MenA) to MenC. (cdc.gov)
  • When carbohydrate is incompletely absorbed by the small bowel, it reaches the colon, where it is fermented by intestinal bacteria. (aappublications.org)
  • Carbohydrates play crucial roles in the immune system, especially in the body's defenses against disease-causing viruses and bacteria. (phys.org)
  • Remarkably, peptides with the most sequence similarity to known epitopes were only slightly more likely to be recognized by the antibody than other random peptides. (mcponline.org)
  • A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of more than 100 different families. (wikipedia.org)
  • Long (20 mer) random sequence peptides were chosen for this study to look at an unbiased sampling of sequence space. (mcponline.org)
  • We explored the ability of two methods singly and in combination to predict the actual epitope from the random sequence peptides bound. (mcponline.org)
  • Peptides with random sequences can be displayed on the surface of phage, and those that bind best to the antibody can be selected and amplified. (mcponline.org)
  • The carbohydrate synthesizer may do the same thing for the emerging fields of glycochemistry and glycobiology - named for carbohydrate sugar chains known as "glycans. (phys.org)
  • Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing. (jic.ac.uk)
  • Amino acid sequences of tryptic 3 H-glycopeptides from HLA-A2 and HLA-B7 were highly homologous. (elsevier.com)
  • May have the capability to bind to carbohydrates. (abcam.com)
  • CSDB provides access to several carbohydrate-related research tools: Simulation of 1D and 2D NMR spectra of carbohydrates (GODDESS: glycan-oriented database-driven empirical spectrum simulation). (wikipedia.org)
  • Here, we report that loss of Drosophila benchwarmer ( bnch ), a predicted lysosomal sugar carrier, leads to carbohydrate storage in yolk spheres during oogenesis and results in widespread accumulation of enlarged lysosomal and late endosomal inclusions. (rupress.org)
  • We hypothesize that, in bnch , defective lysosomal carbohydrate efflux leads to endocytic defects with functional consequences in synaptic strength, neuronal viability, and tau neurotoxicity. (rupress.org)
  • Carbohydrate utilization-related and multidrug transporter nucleic acids and polypeptides, and fragments and variants thereof, are disclosed in the current invention. (patentsencyclopedia.com)
  • N. meningitidis can be identified using Kovac's oxidase test and carbohydrate utilization. (cdc.gov)
  • If the oxidase test is positive, carbohydrate utilization testing should be performed. (cdc.gov)
  • If the carbohydrate utilization test indicates that the isolate may be N. meningitidis , serological tests to identify the serogroup should be performed. (cdc.gov)
  • They contained all the radioactivity and all the carbohydrate of the HLA heavy chain. (elsevier.com)
  • In order to break this chain, you must reduce carbohydrates . (healthcentral.com)
  • So carbohydrates and cholesterol are indeed intimately related, though it requires a somewhat complex chain of events to see the connection. (healthcentral.com)
  • The Lancefield group A carbohydrate consists of a polyrhamnose chain with an immunodominant N- acetylglucosamine side chain ( 9 ) that functions in GAS pathogenesis ( 1 ). (cdc.gov)
  • The six sequenced Geobacillus strains described here have a range of GC contents from 43.9% to 52.5% and clade with named Geobacillus species throughout the entire genus. (frontiersin.org)
  • In this study, we surveyed the sequence variance of rfbT in the Ogawa and Inaba strains in China over a 48-year (1961-2008) period in which serotype shifts occurred among epidemic years. (springer.com)
  • Malectin: a novel carbohydrate-binding protein of the endoplasmic reticulum and a candidate player in the early steps of protein N-glycosylation. (nih.gov)
  • The automated synthesizer puts single sugars, the building blocks of carbohydrates, together like beads on a string. (phys.org)
  • Lectin-like oxidized low-density lipoprotein receptor (LOX-1) has been cloned from human aortic endothelial cells, and has a sequence identical to that from human lung. (biologists.org)
  • The ancestral recipient SE strain was implicated as group C S. equisimilis (GCS/SE) by virtue of the near-identical sequence of the 1,363-bp sequence within GAS/ST128/SE encompassing sections of gacD and gacE homologs (designated as gccD and gccE ) ( Figure 2 ) with GCS and the marked divergence of this 1,363-bp sequence from group G SE (data not shown). (cdc.gov)