Carbohydrate Sequence: The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.Carbohydrate Conformation: The characteristic 3-dimensional shape of a carbohydrate.Oligosaccharides: Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.Carbohydrates: The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Carbohydrate Metabolism: Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.Dietary Carbohydrates: Carbohydrates present in food comprising digestible sugars and starches and indigestible cellulose and other dietary fibers. The former are the major source of energy. The sugars are in beet and cane sugar, fruits, honey, sweet corn, corn syrup, milk and milk products, etc.; the starches are in cereal grains, legumes (FABACEAE), tubers, etc. (From Claudio & Lagua, Nutrition and Diet Therapy Dictionary, 3d ed, p32, p277)Antigens, Tumor-Associated, Carbohydrate: Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.Lectins: Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.Starch: Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.Neisseria gonorrhoeae: A species of gram-negative, aerobic bacteria primarily found in purulent venereal discharges. It is the causative agent of GONORRHEA.T-Lymphocytes, Helper-Inducer: Subpopulation of CD4+ lymphocytes that cooperate with other lymphocytes (either T or B) to initiate a variety of immune functions. For example, helper-inducer T-cells cooperate with B-cells to produce antibodies to thymus-dependent antigens and with other subpopulations of T-cells to initiate a variety of cell-mediated immune functions.Dendritic Cells: Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).Gonorrhea: Acute infectious disease characterized by primary invasion of the urogenital tract. The etiologic agent, NEISSERIA GONORRHOEAE, was isolated by Neisser in 1879.Neisseria: A genus of gram-negative, aerobic, coccoid bacteria whose organisms are part of the normal flora of the oropharynx, nasopharynx, and genitourinary tract. Some species are primary pathogens for humans.Aspergillus fumigatus: A species of imperfect fungi from which the antibiotic fumigatin is obtained. Its spores may cause respiratory infection in birds and mammals.Glycosylphosphatidylinositols: Compounds containing carbohydrate or glycosyl groups linked to phosphatidylinositols. They anchor GPI-LINKED PROTEINS or polysaccharides to cell membranes.Aspergillus: A genus of mitosporic fungi containing about 100 species and eleven different teleomorphs in the family Trichocomaceae.Aspergillosis: Infections with fungi of the genus ASPERGILLUS.Aspergillus nidulans: A species of imperfect fungi from which the antibiotic nidulin is obtained. Its teleomorph is Emericella nidulans.Sialic Acids: A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.Keratan Sulfate: A sulfated mucopolysaccharide initially isolated from bovine cornea. At least two types are known. Type I, found mostly in the cornea, contains D-galactose and D-glucosamine-6-O-sulfate as the repeating unit; type II, found in skeletal tissues, contains D-galactose and D-galactosamine-6-O-sulfate as the repeating unit.Femur Head: The hemispheric articular surface at the upper extremity of the thigh bone. (Stedman, 26th ed)Cartilage, Articular: A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.N-Acetylneuraminic Acid: An N-acyl derivative of neuraminic acid. N-acetylneuraminic acid occurs in many polysaccharides, glycoproteins, and glycolipids in animals and bacteria. (From Dorland, 28th ed, p1518)Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Chromatography, Thin Layer: Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Haptens: Small antigenic determinants capable of eliciting an immune response only when coupled to a carrier. Haptens bind to antibodies but by themselves cannot elicit an antibody response.Glycolipids: Any compound containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety such as an acylglycerol (see GLYCERIDES), a sphingoid, a ceramide (CERAMIDES) (N-acylsphingoid) or a prenyl phosphate. (From IUPAC's webpage)Bibliometrics: The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)Medical Informatics: The field of information science concerned with the analysis and dissemination of medical data through the application of computers to various aspects of health care and medicine.Nursing Informatics: The field of information science concerned with the analysis and dissemination of data through the application of computers applied to the field of nursing.Publications: Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)Ferns: Seedless nonflowering plants of the class Filicinae. They reproduce by spores that appear as dots on the underside of feathery fronds. In earlier classifications the Pteridophyta included the club mosses, horsetails, ferns, and various fossil groups. In more recent classifications, pteridophytes and spermatophytes (seed-bearing plants) are classified in the Subkingdom Tracheobionta (also known as Tracheophyta).Gangliosides: A subclass of ACIDIC GLYCOSPHINGOLIPIDS. They contain one or more sialic acid (N-ACETYLNEURAMINIC ACID) residues. Using the Svennerholm system of abbrevations, gangliosides are designated G for ganglioside, plus subscript M, D, or T for mono-, di-, or trisialo, respectively, the subscript letter being followed by a subscript arabic numeral to indicated sequence of migration in thin-layer chromatograms. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1997)Metabolome: The dynamic collection of metabolites which represent a cell's or organism's net metabolic response to current conditions.Neuraminic AcidsG(M1) Ganglioside: A specific monosialoganglioside that accumulates abnormally within the nervous system due to a deficiency of GM1-b-galactosidase, resulting in GM1 gangliosidosis.G(M3) Ganglioside: A ganglioside present in abnormally large amounts in the brain and liver due to a deficient biosynthetic enzyme, G(M3):UDP-N-acetylgalactosaminyltransferase. Deficiency of this enzyme prevents the formation of G(M2) ganglioside from G(M3) ganglioside and is the cause of an anabolic sphingolipidosis.Peanut Agglutinin: Lectin purified from peanuts (ARACHIS HYPOGAEA). It binds to poorly differentiated cells and terminally differentiated cells and is used in cell separation techniques.Arachis hypogaea: A plant species of the family FABACEAE that yields edible seeds, the familiar peanuts, which contain protein, oil and lectins.Agglutinins: Substances, usually of biological origin, that cause cells or other organic particles to aggregate and stick to each other. They include those ANTIBODIES which cause aggregation or agglutination of particulate or insoluble ANTIGENS.Plant Lectins: Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.Glycomics: The systematic study of the structure and function of the complete set of glycans (the glycome) produced in a single organism and identification of all the genes that encode glycoproteins.Haloferax volcanii: A species of halophilic archaea found in the Dead Sea.Glycosylation: The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.PolysaccharidesPhylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor: Enzymes that catalyze the joining of glutamine-derived ammonia and another molecule. The linkage is in the form of a carbon-nitrogen bond. EC 6.3.5.Vocabulary: The sum or the stock of words used by a language, a group, or an individual. (From Webster, 3d ed)Surgical Mesh: Any woven or knit material of open texture used in surgery for the repair, reconstruction, or substitution of tissue. The mesh is usually a synthetic fabric made of various polymers. It is occasionally made of metal.Rhabditoidea: A superfamily of nematodes of the order RHABDITIDA. Characteristics include an open tube stoma and an excretory system with lateral canals.Vocabulary, Controlled: A specified list of terms with a fixed and unalterable meaning, and from which a selection is made when CATALOGING; ABSTRACTING AND INDEXING; or searching BOOKS; JOURNALS AS TOPIC; and other documents. The control is intended to avoid the scattering of related subjects under different headings (SUBJECT HEADINGS). The list may be altered or extended only by the publisher or issuing agency. (From Harrod's Librarians' Glossary, 7th ed, p163)Haemophilus influenzae: A species of HAEMOPHILUS found on the mucous membranes of humans and a variety of animals. The species is further divided into biotypes I through VIII.Haemophilus Infections: Infections with bacteria of the genus HAEMOPHILUS.Haemophilus: A genus of PASTEURELLACEAE that consists of several species occurring in animals and humans. Its organisms are described as gram-negative, facultatively anaerobic, coccobacillus or rod-shaped, and nonmotile.Sinorhizobium meliloti: A species of gram-negative, aerobic bacteria that causes formation of root nodules on some, but not all, types of sweet clover, MEDICAGO SATIVA, and fenugreek.Ampicillin Resistance: Nonsusceptibility of a microbe to the action of ampicillin, a penicillin derivative that interferes with cell wall synthesis.Streptococcus suis: A species of STREPTOCOCCUS isolated from pigs. It is a pathogen of swine but rarely occurs in humans.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Air Abrasion, Dental: A technique using a pneumatic, high-pressure stream of aluminum oxide to remove DENTAL ENAMEL; DENTIN; and restorative materials from teeth. In contrast to using DENTAL HIGH-SPEED EQUIPMENT, this method usually requires no dental anesthesia (ANESTHESIA, DENTAL) and reduces risks of tooth chipping and microfracturing. It is used primarily for routine DENTAL CAVITY PREPARATION.Brucella suis: A species of gram-negative bacteria, primarily infecting SWINE, but it can also infect humans, DOGS, and HARES.Streptococcal Infections: Infections with bacteria of the genus STREPTOCOCCUS.Pseudomonas aeruginosa: A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.Basement Membrane: A darkly stained mat-like EXTRACELLULAR MATRIX (ECM) that separates cell layers, such as EPITHELIUM from ENDOTHELIUM or a layer of CONNECTIVE TISSUE. The ECM layer that supports an overlying EPITHELIUM or ENDOTHELIUM is called basal lamina. Basement membrane (BM) can be formed by the fusion of either two adjacent basal laminae or a basal lamina with an adjacent reticular lamina of connective tissue. BM, composed mainly of TYPE IV COLLAGEN; glycoprotein LAMININ; and PROTEOGLYCAN, provides barriers as well as channels between interacting cell layers.Erythromycin: A bacteriostatic antibiotic macrolide produced by Streptomyces erythreus. Erythromycin A is considered its major active component. In sensitive organisms, it inhibits protein synthesis by binding to 50S ribosomal subunits. This binding process inhibits peptidyl transferase activity and interferes with translocation of amino acids during translation and assembly of proteins.Bacterial Adhesion: Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.Sialic Acid Binding Immunoglobulin-like Lectins: A family of SIALIC ACID binding proteins found in vertebrate species. They are transmembrane proteins which act as cell surface receptors for a variety of sialylated GLYCOCONJUGATES. While a subset of siglec protein subtypes are evolutionarily conserved between mammalian species, there are many others that are species specific.Sialic Acid Binding Ig-like Lectin 1: A sialic acid binding lectin that was originally identified as an adhesion molecule for inflammatory MACROPHAGES and activated MONOCYTES. This protein is the largest known siglec subtype and contains 16 immunoglobulin C2-set domains. It plays a role in cell to cell interactions and interactions with BACTERIA.Antigens, Differentiation, Myelomonocytic: Surface antigens expressed on myeloid cells of the granulocyte-monocyte-histiocyte series during differentiation. Analysis of their reactivity in normal and malignant myelomonocytic cells is useful in identifying and classifying human leukemias and lymphomas.Cheese: A nutritious food consisting primarily of the curd or the semisolid substance formed when milk coagulates.Lactobacillus delbrueckii: A species of gram-positive, rod-shaped, facultatively anaerobic bacteria. capable of producing LACTIC ACID. It is important in the manufacture of fermented dairy products.Streptococcus thermophilus: A species of thermophilic, gram-positive bacteria found in MILK and milk products.Lactobacillus: A genus of gram-positive, microaerophilic, rod-shaped bacteria occurring widely in nature. Its species are also part of the many normal flora of the mouth, intestinal tract, and vagina of many mammals, including humans. Pathogenicity from this genus is rare.Polysaccharides, Bacterial: Polysaccharides found in bacteria and in capsules thereof.Dermatology: A medical specialty concerned with the skin, its structure, functions, diseases, and treatment.Research: Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)Biology: One of the BIOLOGICAL SCIENCE DISCIPLINES concerned with the origin, structure, development, growth, function, genetics, and reproduction of animals, plants, and microorganisms.Biomedical Research: Research that involves the application of the natural sciences, especially biology and physiology, to medicine.Databases, Chemical: Databases devoted to knowledge about specific chemicals.Physics: The study of those aspects of energy and matter in terms of elementary principles and laws. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Dietary Fiber: The remnants of plant cell walls that are resistant to digestion by the alimentary enzymes of man. It comprises various polysaccharides and lignins.Neisseria meningitidis: A species of gram-negative, aerobic BACTERIA. It is a commensal and pathogen only of humans, and can be carried asymptomatically in the NASOPHARYNX. When found in cerebrospinal fluid it is the causative agent of cerebrospinal meningitis (MENINGITIS, MENINGOCOCCAL). It is also found in venereal discharges and blood. There are at least 13 serogroups based on antigenic differences in the capsular polysaccharides; the ones causing most meningitis infections being A, B, C, Y, and W-135. Each serogroup can be further classified by serotype, serosubtype, and immunotype.Neisseria meningitidis, Serogroup B: Strains of Neisseria meningitidis which are the most common ones causing infections or disease in infants. Serogroup B strains are isolated most frequently in sporadic cases, and are less common in outbreaks and epidemics.Meningococcal Vaccines: Vaccines or candidate vaccines used to prevent infection with NEISSERIA MENINGITIDIS.Meningococcal Infections: Infections with bacteria of the species NEISSERIA MENINGITIDIS.Meningitis, Meningococcal: A fulminant infection of the meninges and subarachnoid fluid by the bacterium NEISSERIA MENINGITIDIS, producing diffuse inflammation and peri-meningeal venous thromboses. Clinical manifestations include FEVER, nuchal rigidity, SEIZURES, severe HEADACHE, petechial rash, stupor, focal neurologic deficits, HYDROCEPHALUS, and COMA. The organism is usually transmitted via nasopharyngeal secretions and is a leading cause of meningitis in children and young adults. Organisms from Neisseria meningitidis serogroups A, B, C, Y, and W-135 have been reported to cause meningitis. (From Adams et al., Principles of Neurology, 6th ed, pp689-701; Curr Opin Pediatr 1998 Feb;10(1):13-8)Silica Gel: A non-crystalline form of silicon oxide that has absorptive properties. It is commonly used as a desiccating agent and as a stationary phase for CHROMATOGRAPHY. The fully hydrated form of silica gel has distinct properties and is referred to as SILICIC ACID.Weapons: Devices or tools used in combat or fighting in order to kill or incapacitate.Chloroform: A commonly used laboratory solvent. It was previously used as an anesthetic, but was banned from use in the U.S. due to its suspected carcinogenicity.Silicon Dioxide: Transparent, tasteless crystals found in nature as agate, amethyst, chalcedony, cristobalite, flint, sand, QUARTZ, and tridymite. The compound is insoluble in water or acids except hydrofluoric acid.Cyclotrons: Devices for accelerating charged particles in a spiral path by a constant-frequency alternating electric field. This electric field is synchronized with the movement of the particles in a constant magnetic field.Fourier Analysis: Analysis based on the mathematical function first formulated by Jean-Baptiste-Joseph Fourier in 1807. The function, known as the Fourier transform, describes the sinusoidal pattern of any fluctuating pattern in the physical world in terms of its amplitude and its phase. It has broad applications in biomedicine, e.g., analysis of the x-ray crystallography data pivotal in identifying the double helical nature of DNA and in analysis of other molecules, including viruses, and the modified back-projection algorithm universally used in computerized tomography imaging, etc. (From Segen, The Dictionary of Modern Medicine, 1992)Spectrometry, Mass, Electrospray Ionization: A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.Mass Spectrometry: An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.Spectroscopy, Fourier Transform Infrared: A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.Trypanosoma brucei brucei: A hemoflagellate subspecies of parasitic protozoa that causes nagana in domestic and game animals in Africa. It apparently does not infect humans. It is transmitted by bites of tsetse flies (Glossina).Variant Surface Glycoproteins, Trypanosoma: Glycoproteins attached to the surface coat of the trypanosome. Many of these glycoproteins show amino acid sequence diversity expressed as antigenic variations. This continuous development of antigenically distinct variants in the course of infection ensures that some trypanosomes always survive the development of immune response to propagate the infection.Protozoan Proteins: Proteins found in any species of protozoan.Membrane Glycoproteins: Glycoproteins found on the membrane or surface of cells.Alkynes: Hydrocarbons with at least one triple bond in the linear portion, of the general formula Cn-H2n-2.Click Chemistry: Organic chemistry methodology that mimics the modular nature of various biosynthetic processes. It uses highly reliable and selective reactions designed to "click" i.e., rapidly join small modular units together in high yield, without offensive byproducts. In combination with COMBINATORIAL CHEMISTRY TECHNIQUES, it is used for the synthesis of new compounds and combinatorial libraries.Azides: Organic or inorganic compounds that contain the -N3 group.Cycloaddition Reaction: Synthetic organic reactions that use reactions between unsaturated molecules to form cyclical products.Cyclization: Changing an open-chain hydrocarbon to a closed ring. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)

Variable domain-linked oligosaccharides of a human monoclonal IgG: structure and influence on antigen binding. (1/5581)

The variable-domain-attached oligosaccharide side chains of a human IgG produced by a human-human-mouse heterohybridoma were analysed. In addition to the conserved N-glycosylation site at Asn-297, an N-glycosylation consensus sequence (Asn-Asn-Ser) is located at position 75 in the variable region of its heavy chain. The antibody was cleaved into its antigen-binding (Fab) and crystallizing fragments. The oligosaccharides of the Fab fragment were released by digestion with various endo- and exoglycosidases and analysed by anion-exchange chromatography and fluorophore-assisted carbohydrate electrophoresis. The predominant components were disialyl- bi-antennary and tetra-sialyl tetra-antennary complex carbohydrates. Of note is the presence in this human IgG of oligosaccharides containing N-glycolylneuraminic acid and N-acetylneuraminic acid in the ratio of 94:6. Furthermore, we determined N-acetylgalactosamine in the Fab fragment of this antibody, suggesting the presence of O-linked carbohydrates. A three-dimensional structure of the glycosylated variable (Fv) fragment was suggested using computer-assisted modelling. In addition, the influence of the Fv-associated oligosaccharides of the CBGA1 antibody on antigen binding was tested in several ELISA systems. Deglycosylation resulted in a decreased antigen-binding activity.  (+info)

Structural characterization of the N-linked oligosaccharides in bile salt-stimulated lipase originated from human breast milk. (2/5581)

The detailed structures of N- glycans derived from bile salt-stimulated lipase (BSSL) found in human milk were determined by combining exoglycosidase digestion with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The N- glycan structures were conclusively determined in terms of complexity and degree of fucosylation. Ion-exchange chromatography with pulsed amperometric detection, together with mass-spectral analysis of the esterified N- glycans, indicated the presence of monosialylated structures. The molecular mass profile of esterified N- glycans present in BSSL further permitted the more detailed studies through collision-induced dissociation (CID) and sequential exoglycosidase cleavages. The N- glycan structures were elucidated to be complex/dibranched, fucosylated/complex/dibranched, monosialylated/complex/dibranched, and monosialylated/fucosylated/dibranched entities.  (+info)

Gas-liquid chromatography of the heptafluorobutyrate derivatives of the O-methyl-glycosides on capillary columns: a method for the quantitative determination of the monosaccharide composition of glycoproteins and glycolipids. (3/5581)

We have developed a method involving the formation of hepta-fluorobutyrate derivatives of O-methyl-glycosides liberated from glycoproteins and glycolipids following methanolysis. The stable derivatives of the most common monosaccharides of these glycoconjugates (Ara, Rha, Xyl, Fuc, Gal, Man, Glc, GlcNAc, GalNAc, Neu5Ac, KDN) can be separated and quantitatively and reproducibly determined with a high degree of sensitivity level (down to 25 pmol) in the presence of lysine as an internal standard. The GlcNAc residue bound to Asn in N-glycans is quantitatively recovered as two peaks. The latter were easily distinguished from the other GlcNAc residues of N-glycans, thus allowing a considerable improvement of the data on structure of N-glycans obtained from a single carbohydrate analysis. The most common contaminants present in buffers commonly used for the isolation of soluble or membrane-bound glycoproteins (SDS, Triton X-100, DOC, TRIS, glycine, and polyacrylamide or salts, as well as monosaccharide constituents of proteoglycans or degradation products of nucleic acids) do not interfere with these determinations. A carbohydrate analysis of glycoproteins isolated from a SDS/PAGE gel or from PDVF membranes can be performed on microgram amounts without significant interferences. Since fatty acid methyl esters and sphingosine derivatives are separated from the monosaccharide peaks, the complete composition of gangliosides can be achieved in a single step starting from less than 1 microg of the initial compound purified by preparative Silicagel TLC. Using electron impact ionization mass spectrometry, reporter ions for the different classes of O-methyl-glycosides (pentoses, deoxy-hexoses, hexoses, hexosamines, uronic acids, sialic acid, and KDN) allow the identification of these compounds in very complex mixtures. The mass of each compound can be determined in the chemical ionization mode and detection of positive or negative ions. This method presents a considerable improvement compared to those using TMS derivatives. Indeed the heptafluorobutyrate derivatives are stable, and acylation of amino groups is complete. Moreover, there is no interference with contaminants and the separation between fatty acid methyl-esters and O-methyl glycosides is achieved.  (+info)

An improved method for the structural profiling of keratan sulfates: analysis of keratan sulfates from brain and ovarian tumors. (4/5581)

A previously developed method for the structural fingerprinting of keratan sulfates (Brown et al., Glycobiology, 5, 311-317, 1995) has been adapted for use with oligosaccharides fluorescently labeled with 2-aminobenzoic acid following keratanase II digestion. The oligosaccharides are separated by high-pH anion-exchange chromatography on a Dionex AS4A-SC column. This methodology permits quantitative analysis of labeled oligosaccharides which can be detected at the sub-nanogram ( approximately 100 fmol) level. Satisfactory calibration of this method can be achieved using commercial keratan sulfate standards. Keratan sulfates from porcine brain phosphocan and human ovarian tumors have been examined using this methodology, and their structural features are discussed.  (+info)

The sialylation of bronchial mucins secreted by patients suffering from cystic fibrosis or from chronic bronchitis is related to the severity of airway infection. (5/5581)

Bronchial mucins were purified from the sputum of 14 patients suffering from cystic fibrosis and 24 patients suffering from chronic bronchitis, using two CsBr density-gradient centrifugations. The presence of DNA in each secretion was used as an index to estimate the severity of infection and allowed to subdivide the mucins into four groups corresponding to infected or noninfected patients with cystic fibrosis, and to infected or noninfected patients with chronic bronchitis. All infected patients suffering from cystic fibrosis were colonized by Pseudomonas aeruginosa. As already observed, the mucins from the patients with cystic fibrosis had a higher sulfate content than the mucins from the patients with chronic bronchitis. However, there was a striking increase in the sialic acid content of the mucins secreted by severely infected patients as compared to noninfected patients. Thirty-six bronchial mucins out of 38 contained the sialyl-Lewis x epitope which was even expressed by subjects phenotyped as Lewis negative, indicating that at least one alpha1,3 fucosyltransferase different from the Lewis enzyme was involved in the biosynthesis of this epitope. Finally, the sialyl-Lewis x determinant was also overexpressed in the mucins from severely infected patients. Altogether these differences in the glycosylation process of mucins from infected and noninfected patients suggest that bacterial infection influences the expression of sialyltransferases and alpha1,3 fucosyltransferases in the human bronchial mucosa.  (+info)

Inhibition of L-selectin-mediated leukocyte rolling by synthetic glycoprotein mimics. (6/5581)

Synthetic carbohydrate and glycoprotein mimics displaying sulfated saccharide residues have been assayed for their L-selectin inhibitory properties under static and flow conditions. Polymers displaying the L-selectin recognition epitopes 3',6-disulfo Lewis x(Glc) (3-O-SO3-Galbeta1alpha4(Fucalpha1alpha3)-6-O-SO3-Glcbeta+ ++-OR) and 3',6'-disulfo Lewis x(Glc) (3, 6-di-O-SO3-Galbeta1alpha4(Fucalpha1alpha3)Glcbeta-OR) both inhibit L-selectin binding to heparin under static, cell-free binding conditions with similar efficacies. Under conditions of shear flow, however, only the polymer displaying 3',6-disulfo Lewis x(Glc) inhibits the rolling of L-selectin-transfected cells on the glycoprotein ligand GlyCAM-1. Although it has been shown to more effective than sialyl Lewis x at blocking the L-selectin-GlyCAM-1 interaction in static binding studies, the corresponding monomer had no effect in the dynamic assay. These data indicate that multivalent ligands are far more effective inhibitors of L-selectin-mediated rolling than their monovalent counterparts and that the inhibitory activities are dependent on the specific sulfation pattern of the recognition epitope. Importantly, our results indicate the L-selectin specificity for one ligand over another found in static, cell-free binding assays is not necessarily retained under the conditions of shear flow. The results suggest that monovalent or polyvalent carbohydrate or glycoprotein mimetics that inhibit selectin binding in static assays may not block the more physiologically relevant process of selectin-mediated rolling.  (+info)

Novel proteoglycan linkage tetrasaccharides of human urinary soluble thrombomodulin, SO4-3GlcAbeta1-3Galbeta1-3(+/-Siaalpha2-6)Galbeta1-4Xyl. (7/5581)

O-linked sugar chains with xylose as a reducing end linked to human urinary soluble thrombomodulin were studied. Sugar chains were liberated by hydrazinolysis followed by N-acetylation and tagged with 2-aminopyridine. Two fractions containing pyridylaminated Xyl as a reducing end were collected. Their structures were determined by partial acid hydrolysis, two-dimensional sugar mapping combined with exoglycosidase digestions, methylation analysis, mass spectrometry, and NMR as SO4-3GlcAbeta1-3Galbeta1-3(+/-Siaalpha2-6)Galbeta1+ ++-4Xyl. These sugar chains could bind to an HNK-1 monoclonal antibody. This is believed to be the first example of a proteoglycan linkage tetrasaccharide with glucuronic acid 3-sulfate and sialic acid.  (+info)

The role of homophilic binding in anti-tumor antibody R24 recognition of molecular surfaces. Demonstration of an intermolecular beta-sheet interaction between vh domains. (8/5581)

The murine antibody R24 and mouse-human Fv-IgG1(kappa) chimeric antibody chR24 are specific for the cell-surface tumor antigen disialoganglioside GD3. X-ray diffraction and surface plasmon resonance experiments have been employed to study the mechanism of "homophilic binding," in which molecules of R24 recognize and bind to other molecules of R24 though their heavy chain variable domains. R24 exhibits strong binding to liposomes containing disialoganglioside GD3; however, the kinetics are unusual in that saturation of binding is not observed. The binding of chR24 to GD3-bearing liposomes is significantly weaker, suggesting that cooperative interactions involving antibody constant regions contribute to R24 binding of membrane-bound GD3. The crystal structures of the Fabs from R24 and chR24 reveal the mechanism for homophilic binding and confirm that the homophilic and antigen-binding idiotopes are distinct. The homophilic binding idiotope is formed largely by an anti-parallel beta-sheet dimerization between the H2 complementarity determining region (CDR) loops of two Fabs, while the antigen-binding idiotope is a pocket formed by the three CDR loops on the heavy chain. The formation of homophilic dimers requires the presence of a canonical conformation for the H2 CDR in conjunction with participation of side chains. The relative positions of the homophilic and antigen-binding sites allows for a lattice of GD3-specific antibodies to be constructed, which is stabilized by the presence of the cell membrane. This model provides for the selective recognition by R24 of cells that overexpress GD3 on the cell surface.  (+info)

*Peanut agglutinin

Lectins recognise and bind particular sugar sequences in carbohydrates; peanut agglutinin binds the carbohydrate sequence Gal-β ... Available Structures of peanut agglutinin Because peanut agglutinin specifically binds a particular carbohydrate sequence it ... GalNAc carbohydrate sequence. The protein is 273 amino acids in length with the first 23 residues acting and a signal peptide ... the binding specificity of peanut agglutinin is used to isolate glycosylated molecules which have the sugar sequence Gal-β(1-3 ...

*Streptococcus suis

The bacteria contain an adhesion protein for the carbohydrate sequence Gal-1,4Gal. After incubation with various amounts of the ... The genome of S. suis isolates from the outbreak were examined to see whether its DNA sequence could explain why these ...

*Eutherian fetoembryonic defense system (eu-FEDS) hypothesis

The major carbohydrate sequence linked to glycodelin-A also profusely coats the surface of schistosomes. The profile of the ... For example, human gametes are coated with carbohydrate sequences that have been implicated in the suppression of NK cell ... There are some notable examples of this mimicry or acquisition of the same carbohydrate sequences implicated in this protective ... The eu-FEDS model further suggests that specific carbohydrate sequences (oligosaccharides) are covalently linked to these ...

*Immunofluorescence

Carbohydrate sequence specificity of the mouse monoclonal antibodies that recognize crossreacting antigens on LOS and human ...

*KLK1

Kellermann J, Lottspeich F, Geiger R, Deutzmann R (1988). "Human urinary kallikrein--amino acid sequence and carbohydrate ... Complete amino acid sequence and sites of glycosylation". Int. J. Pept. Protein Res. 33 (4): 237-49. doi:10.1111/j.1399- ... 1996). "Evaluation of the extent of the binding site in human tissue kallikrein by synthetic substrates with sequences of human ... Lottspeich F, Geiger R, Henschen A, Kutzbach C (1980). "N-Terminal amino acid sequence of human urinary kallikrein homology ...

*Immune tolerance in pregnancy

The eu-FEDS model further suggests that specific carbohydrate sequences (oligosaccharides) are covalently linked to these ...

*Carbohydrate Structure Database

... a unifying sequence format for carbohydrates". Carbohydrate Research. 343 (12): 2162-2171. doi:10.1016/j.carres.2008.03.011. ... CSDB stores structural and spectral data on natural carbohydrates and carbohydrate-related molecules. The main data stored in ... CSDB provides access to several carbohydrate-related research tools: Simulation of 1D and 2D NMR spectra of carbohydrates ( ... Egorova K.S.; Toukach Ph.V. (2013). "Expansion of coverage of Carbohydrate Structure Database (CSDB)". Carbohydrate Research. ...

*NAD(P)(+)-protein-arginine ADP-ribosyltransferase

The enzymes contain ~250-300 residues, which encode putative signal sequences and carbohydrate attachment sites. In addition, ...

*List of MeSH codes (G06)

... carbohydrate sequence MeSH G06.184.603.580 --- conserved sequence MeSH G06.184.603.580.175 --- consensus sequence MeSH G06.184. ... base sequence MeSH G06.184.603.080.040 --- at rich sequence MeSH G06.184.603.080.380 --- gc rich sequence MeSH G06.184.603.080. ... sequence homology, amino acid MeSH G06.184.842.200.820 --- structural homology, protein MeSH G06.184.842.550 --- sequence ... repetitive sequences, nucleic acid MeSH G06.184.603.080.708.330 --- interspersed repetitive sequences MeSH G06.184.603.080. ...

*Carbohydrate-responsive element-binding protein

The protein name derives from the protein's interaction with carbohydrate response element sequences of DNA. This gene encodes ... Carbohydrate-responsive element-binding protein (ChREBP) also known as MLX-interacting protein-like (MLXIPL) is a protein that ... 2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40-5. doi:10.1038/ ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ...

*Laura L. Kiessling

Carbohydrate polymerases mediate the assembly of polysaccharides composed of different sequences and structures. Are the ... "Carbohydrate Research Award for Creativity in Carbohydrate Chemistry , International Carbohydrate Organization". ... "Fluorosugar Chain Termination Agents as Probes of the Sequence Specificity of a Carbohydrate Polymerase". J. Am. Chem. Soc. 134 ... Polymers that co-cluster a receptor on B cells (the B cell receptor) with a carbohydrate-binding protein (CD22) dampen immune ...

*Glycoinformatics

Owing to the lack of a genetic blue print, carbohydrates do not have a "fixed" sequence. Instead, the sequence is largely ... The sequence of branching information in a carbohydrate molecule is represented in the figure. Flitsch SL and Ulijn RV (2003). ... as well as carbohydrate interactions. Conventional usage of the term does not currently include the treatment of carbohydrates ... Carbohydrates or "sugars" (this term should not be confused with simple sugars - monosaccharides and disaccharides) as they are ...

*Glycosyltransferase

The carbohydrate-active enzyme database presents a sequence-based classification of glycosyltransferases into over 90 families ... Sequence-based classification methods have proven to be a powerful way of generating hypotheses for protein function based on ... Carbohydrate chemistry Chemical glycosylation Glucuronosyltransferase Glycogen synthase Glycosyl acceptor Glycosyl donor ... The result of glycosyl transfer can be a carbohydrate, glycoside, oligosaccharide, or a polysaccharide. Some ...

*MMP8

1996). "Amino acid sequence and carbohydrate structure of a recombinant human tissue factor pathway inhibitor expressed in ... one N-and two O-linked carbohydrate chains are located between Kunitz domains 2 and 3 and one N-linked carbohydrate chain is in ... N-terminal sequence determination of the proenzyme and various proteolytically activated forms". Eur. J. Biochem. 189 (2): 295- ... Thomas DB, Davies M, Peters JR, Williams JD (1993). "Tamm Horsfall protein binds to a single class of carbohydrate specific ...

*OLR1

Shi X, Niimi S, Ohtani T, Machida S (2001). "Characterization of residues and sequences of the carbohydrate recognition domain ...

*Glycobiology

... com/medical-breakthroughs/22037-UGA-scientists-team-define-first-ever-sequence-biologically-important-carbohydrate.html Vendor ... contain carbohydrate compounds that can be screened with lectins or antibodies to define carbohydrate specificity and identify ... Raymond Dwek to recognize the coming together of the traditional disciplines of carbohydrate chemistry and biochemistry. This ... contrary to the case with proteins where their amino acid sequence is determined by their corresponding gene. Glycans are ...

*Sialyl-Lewis A

Sialyl-LewisA, also known as sialyl LeA and SLeA, is a tetrasaccharide carbohydrate with the sequence Neu5Acα2-3Galβ1-3[Fucα1-4 ...

*Sialyl-Lewis X

Sialyl LewisX, also known as sialyl LeX or SLeX, is a tetrasaccharide carbohydrate with the sequence Neu5Acα2-3Galβ1-4[Fucα1-3] ... Human ZP is coated with highly dense N- and O-glycans that are terminated with the sialyl-Lewisx sequence. The hemizona assay, ... link) CS1 maint: Multiple names: authors list (link) Clark, G. F. (2013). "The role of carbohydrate recognition during human ... The sialyl Lewis X determinant, E-selectin ligand carbohydrate structure, is constitutively expressed on granulocytes and ...

*Structure validation

CarbohydratesSequences and topology. 44: 39-47. doi:10.1016/j.sbi.2016.11.011. PMID 27940408. Crispin, Max; Stuart, David I ... Around 20% of the deposited carbohydrate structures are in unjustified energy minima. A number of carbohydrate validation web ... see for recommendations on carbohydrate model building and refinement and for reviews on general errors in carbohydrate ... Lütteke, Thomas; von der Lieth, Claus-W (2004). "pdb-care (PDB CArbohydrate REsidue check): a program to support annotation of ...

*Lewis Joel Greene

... amino acid sequence predicted tertiary structure, carbohydrate recognition and analysis of the b-prims fold". Protein Science. ...

*Leguminous lectin family

Amino acid sequences of this loop play an important role in the carbohydrate-binding specificities of these lectins. These ... despite differences in the primary sequences. The carbohydrate-binding sites of these lectins consist of two conserved amino ... X-ray studies have shown that the folding of the polypeptide chains in the region of the carbohydrate-binding sites is also ... Leguminous lectins consist of two or four subunits, each containing one carbohydrate-binding site. The interaction with sugars ...

*Carbohydrate-binding module

Sequence and structural conservation in families CBM17 and CBM28 suggests that they have evolved through gene duplication and ... Carbohydrate-binding module family 20 (CBM20) binds to starch. Carbohydrate-binding module family 21 (CBM21), found in many ... Carbohydrate-binding module family 5 (CBM5) binds chitin. CBM5 and CBM12 are distantly related. Carbohydrate-binding module ... In molecular biology, a carbohydrate-binding module (CBM) is a protein domain found in carbohydrate-active enzymes (for example ...

*Mucorpepsin

Amino acid sequence homology with the other aspartic proteinases, disulfide bond arrangement and site of carbohydrate ...

*Stable-isotope probing

Sequencing the DNA identifies which organisms were consuming existing carbohydrates and which were using carbohydrates more ...

*Sequencing

A practical guide to structural analysis of carbohydrates. ... or whole genome sequencing (sequencing of the all nuclear DNA ... Exome sequencing Full genome sequencing Genetic code Pathogenomics RNA-Seq MicroRNA sequencing Sequence motif Wheeler, David A ... While sequencing DNA gives a genetic profile of an organism, sequencing RNA reflects only the sequences that are actively ... for low-throughput sequencing) and 454 Life Sciences (for high-throughput sequencing). The latter platform sequences roughly ...

*Norovirus

The cloning and sequencing of the Norwalk virus genome showed that these viruses have a genomic organization consistent with ... which contains antigen-presenting sites and carbohydrate-receptor binding regions. The estimated mutation rate (1.21×10−2 to ...
OTHER GLYCAN DEGRADATION GlcNAc GlcNAc Fuc Neu5Ac Gal Asn α2 β1 β1 Man α1 GlcNAc Neu5Ac Gal α2 β1 β1 Man α1 β1 GlcNAc Man β1 β1 GlcNAc β1 α1 Fuc α1 Neu5Ac α2 Neu5Ac α2 Gal β1 Neu5Ac α2 Neu5Ac α2 GalNAc Neu5Ac α2 Neu5Ac α2 Neu5Ac α2 β1 Gal β1 Glc β1 ceramide Ganglioside N-glycan N-Glycan ...
According to National Geographic, the inner core of the Earth ranges between 9,000 and 13,000 degrees Fahrenheit. This is approximately as high as the temperature of the suns...
H. Casajus, S. Tranchimand, D. Wolbert, C. Nugier-Chauvin, S. Cammas-Marion, J. Appl. Polym. Sci., 2016, DOI: 10.1002/app.44604.. • A. Pennec, R. Daniellou, P. Loyer, C. Nugier-Chauvin, V. Ferrieres Carbohydr. Res. 2015, 402, 50-55.. • A. Pennec, L. Legentil, L. Herrera-Estrella, V. Ferrières, A.-L. Chauvin, C. Nugier-Chauvin, Green Chem., 2014, 16, 3803-3809.. • I. Chlubnová, B. Sylla, C. Nugier-Chauvin, R. Daniellou, L. Legentil, B. Králová, V. Ferrières Nat. Prod. Rep., 2011, 28, 937-952.. • I. Chlubnová, D. Filipp, V. Spiwok, H. Dvořáková, R. Daniellou, C. Nugier-Chauvin, B. Králová, V. Ferrières Org. Biomol. Chem., 2010, 8, 2092-2102.. Invited talks at Conferences: ...
The N-linked carbohydrate chains of recombinant human erythropoietin expressed in CHO cells were quantitatively released with peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F, separated from the remaining O-glycoprotein by gel-permeation chromatography, and subsequently fractionated via FPLC on Mono Q, HPLC on Lichrosorb-NH2 and high-pH anion-exchange chromatography on CarboPac PA1. The purified sialylated oligosaccharides were ... read more analyzed by one-dimensional and two-dimensional 500-MHz 1H-NMR spectroscopy. When necessary, oligosaccharides were treated with endo-beta-galactosidase (and N-acetyl-beta-glucosaminidase) followed by 1H-NMR analysis of the incubation products, to obtain additional structural information. Di-, tri-, tri- and tetraantennary, N-acetyllactosamine-type oligosaccharides occur which can be completely (major) or partially (minor) sialylated. Three different types of alpha2-3-linked sialic acids are present, namely, N-acetylneuraminic acid (95%), ...
This application note describes a rapid and robust high-performance anion-exchange chromatography with pulsed amperometric detection method for the accurate determination of common sugars in acid-hydrolyzed biomass samples with high carbohydrate concentrations.
The oligosaccharide chains of microheterogeneous bovine pancreatic DNAases were characterized by the lectin-nitrocellulose sheet method. The active fractions of the DNAases from column chromatography showed four major and several minor spots on a two-dimensional polyacrylamide gel. They were transferred on to nitrocellulose sheets and treated with glycosidases (neuraminidase, endo-beta-N-acetyl glucosaminidase H or F, or peptide N-glycosidase F) and treated with peroxidase-coupled lectins (concanavalin A, Ricinus communis agglutinin or wheat-germ agglutinin). From the results, the most probable oligosaccharide types were proposed to be as follows: the four major spots contained components which had high-mannose type or hybrid-type oligosaccharides, such as those susceptible to endo-beta-N-acetylglucosaminidase H. In addition, spot 1 contained a complex-type biantennary oligosaccharide without sialic acid and spot 3 contained a tri- or tetra-antennary complex-type oligosaccharide with sialic ...
Carbohydrate antigens recognized by "natural" or preformed and elicited antibodies are central to transplantation/transfusion rejection across ABO blood group and species (xenotransplantation) barriers and are also promising candidates for cancer immunotherapy (Ramsland 2005). The key carbohydrate determinants (epitopes) recognized by antibodies are synthesized by a series of intracellular glycosyltransferases and are expressed on the surface of cells as glycolipids and glycoproteins. Often the minimal carbohydrate epitopes are located at the terminal end of more complex oligosaccharide chains, which result in these epitopes being displayed at a wide range of surface densities and contexts (e.g., glycolipids or glycoproteins). For example, many tumor-associated carbohydrate antigens are broadly expressed at very high densities on the cell surface of primary and metastatic tumors, but the same carbohydrates occur at much lower levels and are typically restricted to a few cell types in healthy ...
There are a large number of enzymes that are capable of modifying carbohydrates or carbohydrate derivatives, and that may be used in various analytical methods. The hydrolytic enzymes, which break glycosidic linkages, are useful in the study of disaccharide or polysaccharide structure and in methods for quantitation (Table 9.2). Such enzymes will hydrolyse the glycosidic linkages between the monosaccharide residues and release the individual components for further analysis. The enzyme is chosen bearing in mind the nature of the glycosidic linkage involved, which may not be unique to one particular disaccharide or polysaccharide. Thus a-glucosidase will hydrolyse both the a(l-»4) linkage of maltose and the a( 1-»2) linkage of sucrose, resulting in the release of glucose in both cases.. ...
Read independent reviews on Thermo Scientific™ CarboPac® PA1 Carbohydrate Column from Thermo Fisher Scientific on SelectScience
UPLC, UHPLC, and HPLC-based C18 columns that assist scientists with the analysis and quantitation of important glycan derived components.
Oxygen is the most abundant element in the planet, so it is not unreasonable to expect that it might be one of the dominant "light elements" in the core.. The composition of the Earth"s core remains a mystery. Scientists know that the liquid outer core consists mainly of iron, but it is believed that small amounts of some other elements are present as well.. Now, a new research from a team including Yingwei Fei from Carnegies Geophysical Laboratory has revealed that oxygen does not have a major presence in the outer core.. This has major implications for our understanding of the period when the Earth formed through the accretion of dust and clumps of matter.. According to current models, in addition to large amounts of iron, the Earth"s liquid outer core contains small amounts of so-called light elements, possibly sulfur, oxygen, silicon, carbon, or hydrogen.. The team provides new experimental data that narrow down the identity of the light elements present in Earth"s outer core.. High-speed ...
Supplier pricing and chemical structure of high purity Man(a1-2)-Man(a1-2)-Man(a1-3)-[Man(a1-6)-Man(a1-6)]-Man(b1-4)-GlcNAc(b1-4)-GlcNAc from Omicron Biochemicals, Inc.
Supplier pricing and chemical structure of high purity Man(a1-2)-Man(a1-3)-[Man(a1-6)-Man(a1-6)]-Man(b1-4)-GlcNAc(b1-4)-GlcNAc from Omicron Biochemicals, Inc.
... is a tool which allows to calculate the mass of an oligosaccharide structure [Mass values / Disclaimer]. Note: You can use GlycoMod to predict the possible oligosaccharide structures that occur on proteins from their experimentally determined masses ...
Visit the page of myhomeworkhelp.com and check out their Oligosaccharides Assignment Help team which is ready 24/7 to provide assistance to students.
A. During early development, a snake embryo was exposed to a toxin that interferes with normal migration and differentiation of mesoderm. Name the major structures of the integument most affected by this exposure and how each.
Professional quality Outer Core images and pictures at very affordable prices. With over 20 million stunning photos to choose from we ve got what you need!
Phospholipid patterns of 15 representative strains of the genus Amycolatopsis were recorded by two-dimensional thin-layer chromatography. The structure analysis of the isolated phospholipids was verified by fast atom bombardment-mass spectroscopy. The positive- and negative-ion spectra of the partially purified phospholipid fractions qualitatively reflect their distinctive composition. All strains contained diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylinositol. Two different types of phosphatidylethanolamine and phosphatidylmethylethanolamine were detected, viz., compounds with or without hydroxy fatty acids. These phospholipid patterns underline the integrity of the genus. Fast atom bombardment-mass spectrometry analysis of phospholipid patterns may serve as an aid for differentiation of bacterial species.
TY - JOUR. T1 - Chemical reactions in fast atom bombardment mass spectrometry. AU - Vékey, K.. AU - Zerilli, Luigi F.. PY - 1991. Y1 - 1991. N2 - Examples of various chemical reactions occurring in the matrix or in the selvedge region in fast atom bombardment (FAB) mass spectrometry are discussed. These are categorized as oxidations and reductions; substitutions; clusterings and additions; and sample decomposition or transformation. Some reactions observed showed significant time behaviour and in one case it was possible to determine rate constants. These data suggest that chemical reactions can be accelerated significantly by fast atom bombardment.. AB - Examples of various chemical reactions occurring in the matrix or in the selvedge region in fast atom bombardment (FAB) mass spectrometry are discussed. These are categorized as oxidations and reductions; substitutions; clusterings and additions; and sample decomposition or transformation. Some reactions observed showed significant time ...
Brown, Gavin M. and Huckerby, Thomas N. and Morris, Haydn G. and Abram, Beverly L. and Nieduszynski, Ian A. (1994) Oligosaccharides Derived from Bovine Articular Cartilage Keratan Sulfates after Keratanase II Digestion: Implications for Keratan Sulfate Structural Fingerprinting. Biochemistry, 33 (16). pp. 4836-4846. ISSN 1520-4995 Full text not available from this repository ...
Heparin and heparan sulfate bind a host of basic proteins that take advantage of the sugars dense structural information. The significance of these interactions in various aspects of development, physiology, and disease stimulated keen interest in evaluating structure-activity relationships. The well-defined heparin and heparan sulfate oligosaccharides needed for these studies can be mainly accessed by chemical synthesis and, more recently by chemoenzymatic means. The various synthetic strategies available to chemical synthesis have recently enabled the acquisition of several regular and irregular sequences, including a number of dodecasaccharides, through improved coupling methods and judicial protecting group manipulations. Controlled chain elongation and critical application of modification enzymes allowed the generation of well-defined constructs via chemoenzymatie synthesis. Investigations of various protein interactions with the synthetic constructs delivered valuable informatio
Identification of the methyl phosphate substituent at the non-reducing terminal mannose residue of the O-specific polysaccharides of Klebsiella pneumoniae O3, Hafnia alvei PCM 1223 and Escherichia coli O9/O9a LPS
Eleven tested anions were able to form adducts with neutral oligosaccharides at low cone voltage in negative ion mode electrospray mass spectrometry. Among them, fluoride and acetate have the abilities to significantly enhance the absolute abundance of [M-H]- for neutral oliogosaccharides. The chloride adduct has the best stability among all the adduct species investigated. For the above three anions, CID of adduct species may be used for structural determination of neutral oligosaccharides. In the presence of F- and Ac-, simultaneous detection of acidic oligosaccharides and neutral oligosaccharides was achieved. The ratio of Cl- : non-Cl-containing product ions obtained in CID spectra of chloride adducts of disaccharides was used to differentiate anomeric configurations of disaccharides. Density functional theory (DFT) was employed to evaluate the optimized structures of chloride adducts of disaccharides. The formation and decomposition of chloride adducts with oligosaccharides of different
N-Acetylglucosaminyltransferase III/MGAT3 products available through Novus Biologicals. Browse our N-Acetylglucosaminyltransferase III/MGAT3 product catalog backed by our Guarantee+.
article{fb86bc26-01e6-493b-b144-14ab87d1a4c7, abstract = {The synthesis of the Linear B type 2 trisaccharide (Galalpha3Galbeta4GlcNAc-betaOTMSEt) and the corresponding 2-carboxyethyl beta-thioglycoside is described, as well as coupling of the latter to Sepharose.}, author = {Dahmen, J and Magnusson, Göran and Hansen, HC}, issn = {0732-8303}, keyword = {xenotransplantation,glycoconjugate,glycoside synthesis,thioglycoside}, language = {eng}, number = {3}, pages = {189--199}, publisher = {Marcel Dekker}, series = {Journal of Carbohydrate Chemistry}, title = {Synthesis of the Linear B type 2 trisaccharide Gal alpha 3Gal beta 4GlcNAc beta OTMSEt, and coupling of the corresponding 2-carboxyethyl beta-thioglycoside to Sepharose}, url = {http://dx.doi.org/10.1081/CAR-120004332}, volume = {21}, year = {2002 ...
The structures of hepta- to tetradeca-saccharides, generated by digestion of wheat-endosperm arabinoxylan with endo-(1-,4)-beta-D-xylanase, and isolated by gel-permeation chromatography on Bio-Gel P-6 and high-performance anion-exchange chromatography with pulsed amperometric detection (h.p.a.e.-p.a.d.), were elucidated using monosaccharide and methylation analysis, f.a.b.-m.s., and 1H-n.m.r. spectroscopy. The structures identified had two branching elements, -,4)[alpha-L-Araf-(1-,3)]-beta-D-Xylp-(1-, ... read more and/or -,4)[alpha-L-Araf-(1-,2)][alpha-L-Araf-(1-,3)]-beta-D-Xylp-(1-,, directly connected to each other in all four possible combinations. The h.p.a.e.-p.a.d. elution pattern showed that these combinations are not present in equal amounts. Also, compounds containing two 2,3-branched beta-D-Xylp residues separated by one or two unbranched beta-D-Xylp residues were found, and the presence of a tetradecasaccharide containing three 2,3-branched beta-D-Xylp residues was established. show ...
The oligosaccharides ID in the library are used to refer to specific oligosaccharides for their tandem MS/MS fingerprints and structures and were obtained using their sequential monomeric composition. For example, the oligosaccharide with ID 11100 is made of 1 hexose + 1 HexNAc + 1 fucose + 0 NeuAc + 0 NeuGC.. ...
The Global Soybean Oligosaccharides report provides an elite resource to evaluate the Soybean Oligosaccharides market and support the strategic and preemptive decision-making. The report divulges the matter-of-fact statistics and in-detail examination of the market. It offers a basic layout of the Soybean Oligosaccharides industry, consisting of classifications, applications, definitions, and industry chain structure. It also encompasses a thorough inference of the market and embodies significant insights, facts, and industry-corroborated statistics of the global Soybean Oligosaccharides market.. Furthermore, the report also elaborates several factors concerning the Soybean Oligosaccharides market, comprising key trends, standardization, deployment models, future roadmap, strategies, technologies, value chain, opportunities, ecosystem player profiles, drivers, operator case studies, regulatory landscape, and challenges. Moreover, it also presents an outline regarding the Soybean Oligosaccharides ...
This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase protein family. The encoded enzyme is involved in the biosynthesis of poly-N-acetyllactosamine chains and prefers lacto-N-neotetraose as a substrate. It is a type II transmembrane protein ...
Sialyl Lewis A (sLeA, also known as CA19-9), a tetrasaccharide selectively and highly expressed on advanced adenocarcinomas including colon, stomach, and pancreatic cancers, has long been considered as an attractive target for active and passive vaccination. While progress in antibodies targeting tumor-associated protein antigens resulted in an impressive array of therapeutics for cancer treatment, similar progress in exploiting tumor-associated carbohydrate antigens, such as sLeA, has been hampered by the lack of a detailed understanding of the singular characteristics of these antigens. We have addressed this issue by analyzing antibodies derived from patients immunized with an sLeA/KLH vaccine. These antibodies were engineered to mediate tumor clearance in vivo in preclinical models through Fc-FcγR interactions. However, in contrast to protein antigens in which hFcγRIIIA engagement was both necessary and sufficient to mediate tumor clearance in both preclinical and clinical settings, a ...
Sulfotransferase that utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. Acts on the non-reducing terminal GlcNAc of short carbohydrate substrates. However, it does not transfer sulfate to longer carbohydrate substrates that have poly-N-acetyllactosamine structures. Has no activity toward keratan. Not involved in generating HEV-expressed ligands for SELL. Its substrate specificity may be influenced by its subcellular location ...
keratanase II: an endo-beta-N-acetylglucosaminidase; cleaves the beta(1-3)-glycosidic bond of a fucosylated 6-O-sulfated N-acetylglucosamine
In the few years since the beginning of glycomics in 21th century, many achievements have been made in the study of glycomics, especially in the discovery of sugar genes and the elucidation of the new structure of sugar chain. Although glycomics research has made remarkable achievements in these fields, there are many unknown intermediate links between sugar genes and sugar chains, sugar chains and glycan binding proteins, and there is a general lack of detailed research on the relationship between structure and function.. These fields are not only related to the progress of glycomics itself, but also directly related to the progress of proteome planning, so it has become a major bottleneck in the development of life science.. The advancement of analytical techniques has always been a direct driving force in the development of glycomics. It is the rapid development and wide application of modern instrument analysis technology that lead to the overall prosperity of glycomics research, and inject ...
The MUC-1 antigen is a membrane-anchored mucin-type glycoprotein present in malignant and normal epithelial cells of certain organs, e.g. breast, lung, ovary, pancreas and colon (1). The apoprotein of the MUC-1 mucin contains a transmembrane domain, a cytoplasmic domain, and an extracellular carbohydrate rich domain. The extracellular domain is characterized by polymorphism with respect to the number of a 20 amino acid tandem repeat (VNTR polymorphism). The CanAg CA15-3 EIA is based on two mouse monoclonal antibodies, Ma695 as catcher antibody recognizing a sialylated carbohydrate epitope expressed on the MUC-1 antigen and Ma552 as tracer antibody targeting the PDTRPAPG region of the protein core (2-5).The MUC-1 breast cancer mucin (CA15-3 antigen) is secreted from tumor cells and is a well-established serological marker for monitoring the clinical course of breast cancer patients (6). ...
RNase B is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A and which contains carbohydrate to the extent of 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is consequently considered to be a carbohydrate derivative of RNase A. ( Tarentino, A., Plummer, J., and Maley, F.: Studies on the Oligosaccharide Sequence of Ribonuclease B, J. Biol. Chem., 245, 4150 (1970 ...
The present study tested and confirmed the hypothesis that autoantibodies to aberrant O-glycopeptide epitopes represent a fruitful source of sensitive biomarkers for early detection of cancer. Cancer-associated IgG autoantibodies to several O-glycopeptide epitopes were identified in MUC1, whereas IgG antibodies to peptide epitopes were not detected. The study therefore clearly supports that autoantibody biomarker discovery strategies should include aberrant posttranslational modifications for greatest success. Chemoenzymatic synthesis of cancer-associated O-glycopeptides in combination with a microarray platform was shown to be a feasible strategy for broader analysis of the entire cancer O-glycopeptidome.. Initially, we found an absence of immature nonsialylated MUC1 and MUC16 glycoforms in serum of cancer patients with elevated mucin levels, suggesting that these glycoforms are removed by immune cells or scavenger receptors recognizing immature uncapped glycans. This is in agreement with Varki ...
View Notes - 351 C4 Smith key from CHEM 351 at BYU. Chemistry 351 Fall 2008 Chapter 4 1. Assign the IUPAC name to the following structures. 0k 1,: _Jhfigg~mefi%fimfilm c, 2&4
IN STOCK 13C labeled di-sialylated, galactosylated biantennary N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications
Kilpatrick, D.C. Pusztai, A., Grant, G., Graham, C. and Ewen, S.W (1985) Tomato Lectin Resists Digestion in the Mammalian Alimentary Canal and Binds to Intestinal Villi without Deleterious Effects. FEBS Letters, 185, 299-305.
EMDEX® is a directly compressible, water-soluble tablet binder and filler. Its unique composition of 95% glucose monohydrate and different oligosaccharides derived from starch is monographed under
Shop Oligosaccharide 4-alpha-D-glucosyltransferase ELISA Kit, Recombinant Protein and Oligosaccharide 4-alpha-D-glucosyltransferase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Human Anti-Meningococcal Group A Oligosaccharides IgG negative control serum Serum Controls 600-801-ANC Human Anti-Meningococcal Group A Oligosaccharides IgG negative control serum Serum Controls 600-801-ANC
Background Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X...
Cells All organisms are made of cells. The cell is the smallest unit of living material having major structures within it allowing it to live. Some kinds of organisms are just one cell. This single cell is the organisms entire body. Many organisms are made of more than one cell.
19 janvier 2012. P,The Taleghan fault (TF) is a major active fault of the Central Alborz mountain range in Iran. Located 50 km northwestwards of Tehran, this 80-km-long fault represents one of the major structures threatening 15 million people living in the capital of Iran and the surrounding cities (e.g. (...). Lire la suite. ...
undecaprenyl phosphate-α-L-Ara4N: 4-amino-4-deoxy-β-L-arabinosyltransferase (EC 2.4.2.43); dodecaprenyl phosphate-β-galacturonic acid: lipopolysaccharide core α-galacturonosyl transferase (EC 2.4.1.- ...
undecaprenyl phosphate-α-L-Ara4N: 4-amino-4-deoxy-β-L-arabinosyltransferase (EC 2.4.2.43); dodecaprenyl phosphate-β-galacturonic acid: lipopolysaccharide core α-galacturonosyl transferase (EC 2.4.1.- ...
For an organism to develop and function, the individual cells must exchange information, or communicate, with each other. Is it possible to learn their language and
Methods to allow the clean preparation of oligosaccharides were investigated using techniques that do not require conventional column chromatography or an aqueous work-up. The route was designed to provide rapid access to oligosaccharides and is suit
In this 3 volume collection focusing on glycomics, readers will appreciate how such discoveries were made and how such methods can be applied for readers...
In previous studies, we have shown that the highly sialylated renal anti-adhesin Podocalyxin (PC) is expressed in the developing brain [13], [14 ...
We explain Disaccharides with video tutorials and quizzes, using our Many Ways(TM) approach from multiple teachers.|p| This lesson will introduce disaccharides, including an overview of their structure, and providing multiple examples.|/p|
List of words make out of Monosaccharides. All anagrams of Monosaccharides. Words made after unscrambling Monosaccharides. Scrabble Points.
A recent study by the Pioneer Institute confirms what most parents and educators already knew: Common Core is the leading killer of education achievement and innovation weve seen in decades.
Does anyone know any good links to a site with a detailed drawing/photograph/diagram of a eukaryotic cells as seen under and electron microscope with the following structures ...
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Bovine prothrombin contains three asparagine-linked sugar chains in 1 molecule. The sugar chains were quantitatively released from the polypeptide backbone by hydrazinolysis. All of the oligosaccharides thus obtained contain N-acetylneuraminic acid.
Oligosaccharides and polysaccharides are an important class of polymeric carbohydrates found in virtually all living entities. Their structural features make their nomenclature challenging and their roles in living systems make their nomenclature important. Oligosaccharides are carbohydrates that are composed of several monosaccharide residues joined through glycosidic linkage, which can be hydrolyzed by enzymes or acid to give the constituent monosaccharide units. While a strict definition of an oligosaccharide is not established, it is generally agreed that a carbohydrate consisting of two to ten monosaccharide residues with a defined structure is an oligosaccharide. Some oligosaccharides, for example maltose, sucrose, and lactose, were trivially named before their chemical constitution was determined, and these names are still used today. Maltose Sucrose Lactose Trivial names, however, are not useful for most other oligosaccharides and, as such, systematic rules for the nomenclature of ...
The heparin hexasaccharide MS standard 6 has been purified as one defined sequence and structure. It is not a mix of oligosaccharides. It is a heparin hexasaccharide with six sulfates attached to a repeating structure of α(1-4) linked Uronic Acid (UA) β(1-4) linked to N-Acetylglucosamine (GlcNAc) or N-sulfylglucosamine (GlcNS). The exact structure of the heparin hexasaccharide MS standard 6 is ∆UA2S - GlcNS6S -UA- GlcNAc6S-IdoA - GlcNS6S. The uronic acid residue may be either glucuronic or iduronic acid. It has an exact mass of 1533.05 ...
TY - JOUR. T1 - Structural characterization of multibranched oligosaccharides from seal milk by a combination of off-line high-performance liquid chromatography-matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry and sequential exoglycosidase digestion. AU - Kinoshita, Mitsuhiro. AU - Ohta, Hiroko. AU - Higaki, Kanata. AU - Kojima, Yoko. AU - Urashima, Tadasu. AU - Nakajima, Kazuki. AU - Suzuki, Minoru. AU - Kovacs, Kit M.. AU - Lydersen, Christian. AU - Hayakawa, Takao. AU - Kakehi, Kazuaki. PY - 2009/5/15. Y1 - 2009/5/15. N2 - A complex mixture of diverse oligosaccharides related to the carbohydrates in glycoconjugates involved in various biological events is found in animal milk/colostrum and has been challenging targets for separation and structural studies. In the current study, we isolated oligosaccharides having high molecular masses (MW ∼ 3800) from the milk samples of bearded and hooded seals and analyzed their structures by off-line ...
TY - JOUR. T1 - Isolation and identification of a choline-linked mannobiose in the glycoproteins of fusarium sp. M7-1. AU - Iwahara, Shojiro. AU - Suemori, Nahoko. AU - Takegawa, Kaoru. PY - 1996/1/1. Y1 - 1996/1/1. N2 - An unidentified oligosaccharide was isolated from an oligomer mixture derived by alkaline borohydride treatment from glycoproteins of Fusarium sp. M7-1. The isolated compound was identified as O-α-d-Mannopyranosyl (1 → 2)-d-Mannitol-6-phosphocholine by NMR and Ms spectrometry.. AB - An unidentified oligosaccharide was isolated from an oligomer mixture derived by alkaline borohydride treatment from glycoproteins of Fusarium sp. M7-1. The isolated compound was identified as O-α-d-Mannopyranosyl (1 → 2)-d-Mannitol-6-phosphocholine by NMR and Ms spectrometry.. UR - http://www.scopus.com/inward/record.url?scp=0030073020&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0030073020&partnerID=8YFLogxK. U2 - 10.1271/bbb.60.349. DO - 10.1271/bbb.60.349. M3 - ...
Oligosaccharides and polysaccharides are composed of longer chains of monosaccharide units bound together by glycosidic bonds. The distinction between the two is based upon the number of monosaccharide units present in the chain. Oligosaccharides typically contain between three and ten monosaccharide units, and polysaccharides contain greater than ten monosaccharide units. Definitions of how large a carbohydrate must be to fall into each category vary according to personal opinion. Examples of oligosaccharides include the disaccharides mentioned above, the trisaccharide raffinose and the tetrasaccharide stachyose.. Oligosaccharides are found as a common form of protein posttranslational modification. Such posttranslational modifications include the Lewis and ABO oligosaccharides responsible for blood group classifications and so of tissue incompatibilities, the alpha-Gal epitope responsible for hyperacute rejection in xenotransplantation, and O-GlcNAc modifications.. Polysaccharides represent an ...
Permeabilization of the plasma membrane of HepG2 cells subsequent to pulse-chase incubations has enabled us to evaluate the hypothesis that cytosolic-free oligosaccharides are sequestered into and degraded by lysosomes. Short chase incubations revealed that free oligosaccharides rapidly appear in the cytosol at a time during which there is a loss of these components from the MBCs (Fig. 1, A and B). This observation can be accounted for by the previously observed rapid translocation of large, free polymannose-type oligosaccharides out of the ER into the cytosol (Moore et al., 1995). At present it is unclear whether this ER-to-cytosol transport of free oligosaccharides is the sole mechanism responsible for the appearance of free oligosaccharides in the cytosol. Recently, it has been proposed that newly synthesized glycoproteins may be translocated out of the ER and degraded in this compartment (Wiertz et al., 1996) by the actions of a cytosolic N-glycanase (Kitajima et al., 1995; Suzuki et al., ...
IVKOVC, M. et al. Effects of a novel carbohydrate fraction on broiler performance and intestinal function. S. Afr. j. anim. sci. [online]. 2012, vol.42, n.2, pp.131-138. ISSN 2221-4062.. This study was performed to determine the effects of a natural yeast-based feed ingredient (natural carbohydrate fraction (NCF) isolated from a specific strain of yeast) on broiler chickens, and to examine its mode of action. The trial was set up as a complete randomized design with three treatments and eight replicates (38 Ross 308 chickens per pen). Two levels of NCF, 0.2 g/kg and 0.4 g/kg, were compared to a negative control. The NCF addition increased body weight during the initial period, but this benefit was lost towards the end of the trial. Feed conversion ratio was improved significantly with 0.4 g NCF/kg (1.79 compared with 1.83 in control group). Mortality was numerically lower in the groups receiving NCF. Significant effects on caecal bacterial population were not found. Intestine length and weight ...
Carbohydrates constitute one of the three major classes of biomolecules found in all living cells and, unlike nucleic acids and proteins, their polymeric structures are not based on a template. The structural diversity of these molecules confers them an enormous capacity to encode information in biological systems, acting as efficient mediators in the interaction of the cell with the environment. In order to understand the roles of glycans in biological processes it is of key importance to have a detailed understanding of their structures and conformational preferences, and NMR spectroscopy is one of most powerful techniques for the study of these molecules in solution.. This thesis is focused on the structural and conformational analysis of lipopolysaccharides from Gram-negative bacteria. In the first two projects (Chapter 2 and 3) the structural analyses of the biological repeating units of the O-antigen polysaccharides from E. coli O174ab and O115 are described; in both cases a combination of ...
Asparagine-linked sugar chains of glycoproteins in calf thymocyte plasma membrane. Isolation and fractionation of oligosaccharides liberated by hydrazinolysis.:Isolation and Fractionation of Oligosaccharides Liberated by Hydrazinolysis (1980 ...
Glycan microarrays, carrying hundreds of different sugars on chip surfaces, have become a standard tool for the study of interactions of biomolecules with carbohydrates. The chip-based format offers important advantages, including the ability to screen in parallel several thousand binding events on a single slide, the minimal amount of sample required for one experiment, and the multivalent display of sugars on the chip that mimics the presentation of carbohydrates in nature. This chapter presents recent advances and future challenges in glycan microarray technology. We describe different immobilization and detection methods as well as applications in glycomics, drug discovery, and biomedicine ...
Various biological effects have been reported for sulfated chitosan oligosaccharides, but the molecular mechanisms of action of their anti-inflammatory effects are still unknown. This study aimed to evaluate the anti-inflammatory effects of sulfated chitosan oligosaccharides and to elucidate the possible mechanisms of action. The results showed that pretreated low molecular weight sulfated chitosan oligosaccharides inhibited the production of nitric oxide (NO) and inflammatory cytokines such as IL-6 and TNF-α in lipopolysaccharide (LPS)-activated RAW264.7 cells. The sulfated chitosan oligosaccharides also suppressed inducible nitric oxide synthase (iNOS), phosphorylation of JNK and translocation of p65, a subunit of NF-κB, into the nucleus by inhibiting degradation of IκB-α. Our investigation suggests sulfated chitosan oligosaccharides inhibit IL-6/TNF-α in LPS-induced macrophages, regulated by mitogen-activated protein kinases (MAPKs) pathways dependent on NF-κB activation.
In the few years since the beginning of glycomics in 21th century, many achievements have been made in the study of glycomics, especially in the discovery of sugar genes and the elucidation of the new structure of sugar chain. Although glycomics research has made remarkable achievements in these fields, there are many unknown intermediate links between sugar genes and sugar chains, sugar chains and glycan binding proteins, and there is a general lack of detailed research on the relationship between structure and function.. These fields are not only related to the progress of glycomics itself, but also directly related to the progress of proteome planning, so it has become a major bottleneck in the development of life science.. The advancement of analytical techniques has always been a direct driving force in the development of glycomics. It is the rapid development and wide application of modern instrument analysis technology that lead to the overall prosperity of glycomics research, and inject ...
Our group is interested in chemical glycobiology.. Total synthesis is combined with an enzymatic approach to prepare complex carbohydrates, glycopeptides and semi-synthetic glycoproteins. By using synthetic molecules structural details in protein-carbohydrate interactions are explored, which are relevant in infections, inflammation, cancer and aging. In addition to understand disease mechanisms, an ultimate aim in our projects is to develop glycomimetic inhibitors to block specific protein-carbohydrate interactions for instance to address the current problems with antimicrobial resistance in airway disease infections. We further apply synthetic glycopeptides and semi-synthetic glycoproteins to develop glycoproteomic tools for improved enrichment and characterization of glycan structural isomers of glycoproteins and for studies of new PTMs. Small differences in carbohydrate structure has an enormous impact on biological function, for instance in turning on or off an inflammatory pathway and makes ...
Anthraquinone-lectin hybrids were effectively synthesized using water-soluble anthraquinone derivative with concanavalin A (ConA) and hygrophorus russula lectin (HRL) to give anthraquinone-ConA () and anthraquinone-HRL (17) hybrids, respectively. These anthraquinone-lectin hybrids effectively and selectively degraded oligosaccharides containing a mannose residue as a non-reducing terminal sugar, which has affinity for ConA and HRL, under photo-irradiation with long-wavelength UV light without additives and under neutral conditions. In addition, anthraquinone-HRL (17) selectively photo-degraded only Man(α1,6)Man, which has a high affinity for HRL, among several mannosides by recognition of both the type and glycosidic linkage profile of the sugar in an oligosaccharide. ...
The tumor marker CA242 is defined by the monoclonal antibody C242. The chemical structure of the antigenic determinant is not exactly known, but the determinant have been shown to be a sialylated carbohydrate structure. In serum, CA242 is found on the same mucin-complex as CA50 and sialylated Lewisa (CA19-9). Thus, CA242 is related, but not identical to the epitope of CA19-9 (1, 2). Serum levels of CA242 are low in healthy subjects and subjects with benign diseases, while elevated levels are commonly found in serum from patients with gastro-intestinal cancer (3). The CA242 marker may be used as an aid in the diagnosis and management of patients with known or suspected gastro-intestinal carcinomas (4-9). The CanAg CA242 EIA should not be used as a substitute for any established clinical examination of malignancy, but may be used as a complement to existing clinical and laboratory methods. ...
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POLYSACCHARIDE STRUCTURE. References. Tombs, M .P. & Harding, S.E., An Introduction to Pol ysaccharide Biotechnology, Taylor & Francis, London, 1997 D.A. Rees, Polysaccharide Shapes, Chapman & Hall, 1977 Slideshow 198522 by johana
With the implementation of GlyTouCan the mission of GlycomeDB comes to an end. GlyTouCan is the international glycan structure repository. This repository is a freely available, uncurated registry for glycan structures that assigns globally unique accession numbers to any glycan. All glycan structures and associates information (cross references and taxon annotation) have been migrated to GlyTouCan and are available in this database in addition many more structures. GlyTouCan allows individual researchers to register their own structures and associate these structures with papers. GlyTouCan is supported by the Japanese Ministry of Education, Culture, Sports, Science & Technology and has been developed by researchers at the Soka University and the Complex Carbohydrate Research Center. Please use GlyTouCan and the unique glycan accession numbers for your work and help us to make GlyTouCan a comprehensive namespace for glycan structures. In case of questions or comments please contact Rene ...
With the implementation of GlyTouCan the mission of GlycomeDB comes to an end. GlyTouCan is the international glycan structure repository. This repository is a freely available, uncurated registry for glycan structures that assigns globally unique accession numbers to any glycan. All glycan structures and associates information (cross references and taxon annotation) have been migrated to GlyTouCan and are available in this database in addition many more structures. GlyTouCan allows individual researchers to register their own structures and associate these structures with papers. GlyTouCan is supported by the Japanese Ministry of Education, Culture, Sports, Science & Technology and has been developed by researchers at the Soka University and the Complex Carbohydrate Research Center. Please use GlyTouCan and the unique glycan accession numbers for your work and help us to make GlyTouCan a comprehensive namespace for glycan structures. In case of questions or comments please contact Rene ...
The objective of this investigation was primarily to establish that the apparent increase in serum fucose levels in patients with malignant tumors is real and to obtain data as to its source. Carbohydrate residues (L-fucose, D-mannose, D-galactose) were estimated by borate ion-exchange chromatography after mild acid hydrolysis of serum proteins. Data are presented which confirm reported increases in levels of serum fucose in certain malignancies and indicate that these increases may be attributed to quantitative alterations in specific serum glycoproteins observed in the glycoprotein profile associated with cancer. Values for serum fucose levels in pathological and nonpathological sera obtained by various analytical methods are presented and discussed. (Author)
An ideal way for the analysis of amylase by PGC chromatography. differ, for instance, with the existence/lack of fucose, galactose, or even a bisecting GlcNAc, as well as the deviations could be bigger, but modification with elements for the particular nearest neighbor will produce reasonably great approximations of accurate values (Desk ?(Desk2).2). Huge differences in retention period and solvent structure might decrease the gain in correction hence. For example, the acetonitrile articles changes by one factor of just one 1.25 through the Rabbit Polyclonal to OR10A5 elution from the trisialoglycans (Fig.?2) and therefore can lead to deviations that, however, appear little weighed against the huge first mistake. Fig. 2 PGC-LC splitting up of triantennary, trisialylated symbolize 13C atoms within the chemical substance formulas. galactose, galactose 1-phosphate, blood sugar GnGn (i.electronic., a desialylated and degalactosylated diantennary N-glycan) was incubated with UDP-13 C 6-galactose and ...
Sugar moieties on the cell surface play one of the most important roles in cellular recognition. In order to elucidate the molecular mechanism of these cellular phenomena, assessment of the structure...
Description: The chemical reactions and pathways resulting in the formation of glycoproteins, any protein that contains covalently bound glycose (i.e. monosaccharide) residues; the glycose occurs most commonly as oligosaccharide or fairly small polysaccharide but occasionally as monosaccharide.. ...
Highly glycosylated, polymeric, secreted mucins such asMUC2 serve to coat and protect the gastrointestinal, reproductive, and respiratory tracts. Given their role in protection, large size, and the complex processes required for their biosynthesis, it is not surprising that alterations in mucins, and their levels of expression, are associated with several diseases. In certain cancers, mucin oligosaccharides are altered in several ways, including a reduction in number, shortening, the exposure of normally cryptic inner chain residues, and the expression of novel carbohydrate structures (21, 23). The levels and types of mucins expressed are altered in cancers as well. For example, human cancers, especially colon cancers, differ widely in the levels ofMUC2 they express (18). Most colon cancers express only low levels ofMUC2 compared with normal colon, but a class known as mucinous colon cancers expresses high levels ofMUC2, suggesting that they follow a different mutational pathway to malignancy ...
Sigma-Aldrich offers abstracts and full-text articles by [Christina P Wang, Vincent C Hascall, Fuming Zhang, Robert J Linhardt, Amina Abbadi, Aimin Wang].
Oligosaccharides and polysaccharides are both complex carbohydrates. Their building blocks are the dietary monosaccharides glucose, fructose and galactose,...
I have done LPS extraction using the hot phenol-water method which was originally decribed by Westphal, O. & Jann, K. (1965) Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. Methods Carbohydr Chem 5: 83-91. I used a modification of it to extract Neisseria gonorrhoeae LPS (Mol Microbiol 2001 42: 167) which worked pretty well. If you need a more detailed protocol I could send you that ...
Aoki-Kinoshita K, Agravat S, Aoki NP, Arpinar S, Cummings RD, Fujita A, Fujita N, Hart GM, Haslam SM, Kawasaki T, et al. GlyTouCan 1.0--The international glycan structure repository. Nucleic Acids Res. 2016;44 (D1) :D1237-42.
A tetrasaccharide is a carbohydrate which gives upon hydrolysis four molecules of the same or different monosaccharides. For example, stachyose upon hydrolysis gives one molecule each of glucose and fructose and two molecules of galactose. The general formula of a tetrasaccharide is typically C24H42O21 ...
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A rare CaCO3 modification that is metastable below approx. 400°C. May be stabilised by sulphate (Fernández-Díaz et al., 2010). Vaterite is actually composed of at least two different crystallographic structures that coexist within a pseudo-single crystal. The major structure exhibits hexagonal symmetry; the minor structure, existing as nanodomains within the major matrix, is still unknown (Kabalah-Amitai et al., 2013). The structure is disordered in terms of (1) different orientations of the carbonate groups, (2) different stacking sequences of the carbonate-comprising layers, and (3) possible chiral forms (Demichelis et al., 2013). The OD character leads to polytypism; the OD layer comprises Ca coordination polyhedra and halves of the carbonate groups, and the group symmetry of the layer is C2/m; the known stacking sequences include: P6122, P6522, C2/c, C2/c2/m21/m
Enzymatic Protein Deglycosylation Kit: The E-DEGLY Kit contains all the enzymes and reagents needed to completely remove all N-linked and simple O-linked carbohydrates from glycoproteins, as well as cleave complex core O-linked carbohydrates including those containing polylactosamine.
シビアな構造異常糖タンパク質が出現すると手順を踏まずに強制分解 -酵母にはない高等動物特有の小胞体糖タンパク質分解システムの解明-. 京都大学プレスリリース. 2015-11-20.
Biomedica is proud to be the exclusive partner of Glycotechnica offering Glycomics - Simplified Glycoanalysis in Europe. This new technology - based on LecChip™ (Lectin microarrays) - enables quick and accurate glycan profiling for any complex glycans, even for mixtures.
The goal of the proposed research is to develop a general and highly efficient chemo enzymatic method for making diverse homogeneous glycopeptides and glycoprot...
Carbohydrates All carbohydrates have the basic chemical structure of CH2O. The differences lie in the number of times that basic structure is repeated, i.e. C6H12O6, the chemical formula of glucose. Other compounds can have the same chemical formula however, the way the molecules are linked to each other is different. The difference gives them each…
Des oligosaccharides végétaux constituent des signaux de défense des plantes contre les attaques de pathogènes. Des oligosaccharines influencent également la croissance des végétaux. (fre) ...
This page provides complete information on Carbohydrates,functions of Carbohydrates,Types of Carbohydrates, Structure of Carbohydrates.
Non-typeable Haemophihis influenzae (NTHi) is a significant cause of otitis media in children. We have employed single and multiple step electrospray ionization mass spectrometry (ESIMS) and NMR spectroscopy to profile and elucidate lipopolysaccharide (LPS) structural types expressed by NTHi strain 162, a strain obtained from an epidemiological study in Finland. ESIMS on O-deacylated LPS (LPS-OH) and core oligosaccharide (OS) samples of LPS provided information on the composition and relative abundance of glycoforms differing in the number of hexoses linked to the conserved inner-core element, L-alpha-D-Hepp-(1--, 2)-[PEtn --, 6]-L-alpha-D-Hepp-(1 --, 3)-L-alpha-D-Hepp-(1 --, 5)(.)[PPEtn --, 4]-alpha-Kdop-(2 --, 6)-Lipid A of H. influenzae LPS. The strain examined was found to elaborate Hex2 to Hex5 LPS glycoform populations having structures identical to those observed for H. influenzae strain Rd [Risberg, A.; Masoud, H.; Martin, A.; Richards, J.C.; Moxon, E.R.; Schweda, E.K.H. Eur. J Biochem. ...
We have previously described the structures of neutral and sialylated O-glycosidic mannose-linked tetrasaccharides and keratan sulphate polysaccharide chains in the chondroitin sulphate proteoglycan of brain. The present paper provides information on a series of related sialylated and/or sulphated tri- to penta-saccharides released by alkaline-borohydride treatment of the proteoglycan glycopeptides. The oligosaccharides were fractionated by ion-exchange chromatography and gel filtration, and their structural properties were studied by methylation analysis and fast-atom-bombardment mass spectrometry. Five fractions containing [35S]sulphate-labelled oligosaccharides were obtained by ion-exchange chromatography, each of which was eluted from Sephadex G-50 as two well-separated peaks. The apparent Mr values of both the large- and small-molecular-size fractions increased with increasing acidity (and sulphate labelling) of the oligosaccharides. The larger-molecular-size fractions contained short ...
Virulence of Vibrio vulnificus has been strongly associated with encapsulation and an opaque colony morphology. Capsular polysaccharide was purified from a whole-cell, phosphate-buffered saline-extracted preparation of the opaque, virulent phase of V. vulnificus M06-24 (M06-24/O) by dialysis, centrifugation, enzymatic digestion, and phenol-chloroform extraction. Nuclear magnetic resonance spectroscopic analysis of the purified polysaccharide showed that the polymer was composed of a repeating structure with four sugar residues per repeating subunit: three residues of 2-acetamido-2,6-dideoxyhexopyranose in the alpha-gluco configuration (QuiNAc) and an additional residue of 2-acetamido hexouronate in the alpha-galactopyranose configuration (GalNAcA). The complete carbohydrate structure of the polysaccharide was determined by heteronuclear nuclear magnetic resonance spectroscopy and by high-performance anion-exchange chromatography. The 1H and 13C nuclear magnetic resonance spectra were completely ...
The structure of the O-antigen polysaccharide (PS) from the enteroaggregative Escherichia coli strain 180/C3 has been determined. Sugar and methylation analysis together with 1H and 13C NMR spectroscopy were the main methods used. The PS is composed of tetrasaccharide repeating units with the following structure:→2)-β-d-Quip3NAc-(1→3)-β-d-Ribf-(1→4)-β-d-Galp-(1→3)-α-d-GalpNAc-(1→Analysis of NMR data indicates that the presented sequence of sugar residues also represents the biological repeating unit of the O-chain. The structure is closely related to that of O-antigen polysaccharide from E. coli O5 and partially to that of E. coli O65. The difference between the O-antigen from the 180/C3 strain and that of E. coli O5 is the linkage to the d-Quip3NAc residue, which in the latter strain is 4-O-substituted. The E. coli O65 O-antigen contains as part of its linear pentasaccharide repeating unit a similar structural element, namely ...
|p|Liquid state, high resolution 13C NMR spectroscopy and mass spectrometry were used to study the composition and structure of soil organic matter (SOM) using soil extracts from two long-term experiments at the Rothamsted Experimental Station. Both one- and two-dimensional NMR techniques were applied. 13C NMR sub-spectra of the CHn (n=0...3) groups, obtained by the Distortionless Enhancement by Polarisation Transfer (DEPT) technique, were used for the elucidation of the qualitative and quantitative composition of humic and fulvic acids in the soils. The chemical structure of SOM was further analysed at the molecular level through Fast Atom Bombardment Mass Spectrometry (FABMS) and Gas Chromatography-Mass Spectrometry (GC/MS). Humic and fulvic extract results were not only compared to each other, but also to the solid state 13C NMR results for the complete soil sample.|/p|
Leukocyte adhesion deficiency/congenital disorder of glycosylation IIc (LAD II/CDG IIc) is a genetic disease characterized by a decreased expression of fucose in glycoconjugates, resulting in leukocyte adhesion deficiency and severe morphological and neurological abnormalities. The biochemical defect is a reduced transport of guanosine diphosphate-L-fucose (GDP-L-fucose) from cytosol into the Golgi compartment, which reduces its availability as substrate for fucosyltransferases. The aim of this study was to determine the effects of a limited supply of GDP-L-fucose inside the Golgi on core fucosylation (a1,6-fucose linked to core N-acetylglucosamine [GlcNAc]) of N-linked glycans in LAD II fibroblasts. The results showed that, although [3H]fucose incorporation was generally reduced in LAD II cells, core fucosylation was affected to a greater extent compared with other types of fucosylation of N-linked oligosaccharides. In particular, core fucosylation was found to be nearly absent in biantennary ...

Carbohydrate SequencingCarbohydrate Sequencing

An easy-to-use directory for life science and biomedical research products. Find special deals on products, order catalogs and browse product lines from suppliers of reagents, antibodies, laboratory equipment, and more.
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Encoding schemes used in various carbohydrate sequence  | Open-iEncoding schemes used in various carbohydrate sequence | Open-i

Encoding schemes used in various carbohydrate sequence databases. The N-glycan core structure has been chosen to illustrate the ... Figure 2: Encoding schemes used in various carbohydrate sequence databases. The N-glycan core structure has been chosen to ... Figure 2: Encoding schemes used in various carbohydrate sequence databases. The N-glycan core structure has been chosen to ... Conclusion: GlycomeDB is a new, publicly available database for carbohydrate sequences with a unified, all-encompassing ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC2567997_1471-2105-9-384-2&req=4

Glycosciences.de LINUCS - LInear Notation for Unique description of Carbohydrate SequencesGlycosciences.de LINUCS - LInear Notation for Unique description of Carbohydrate Sequences

LINUCS: LInear Notation for Unique description of Carbohydrate Sequences Introduction. LINUCS was chosen to fulfil to following ... LINUCS: linear notation for unique description of carbohydrate sequences.. Carbohydr Res 2001, 336: 1-11.. ... The first step is to transform the carbohydrate in SWEET-notation and the second step is to make it unique. ... Primary structure of the carbohydrate chain of soybean agglutinin. A reinvestigation by high resolution 1H NMR spectroscopy. J ...
more infohttp://glycosciences.de/tools/linucs/

Advancing solutions to the carbohydrate sequencing challenge :: MPG.PuReAdvancing solutions to the carbohydrate sequencing challenge :: MPG.PuRe

Carbohydrate, NMR, mass spectrometry, liquid chromatography, ion-mobility spectrometry, infrared,br/,spectroscopy; Title: ... Advancing solutions to the carbohydrate sequencing challenge Gray, C. J., Migas, L. G., Barran, P. E., Pagel, K., Seeberger, P ... 2019). Advancing solutions to the carbohydrate sequencing challenge. Journal of the American Chemical Society. doi:10.1021/jacs ... Free keywords: Glycan, Carbohydrate, NMR, mass spectrometry, liquid chromatography, ion-mobility spectrometry, infrared ...
more infohttps://pure.mpg.de/pubman/faces/ViewItemFullPage.jsp?itemId=item_3152949

Prediction of Carbohydrate-Binding Proteins from Sequences Using Support Vector MachinesPrediction of Carbohydrate-Binding Proteins from Sequences Using Support Vector Machines

... Seizi Someya,1 Masanori Kakuta,1 ... Carbohydrate-binding proteins are proteins that can interact with sugar chains but do not modify them. They are involved in ... Our method is based on support vector machines (SVMs). We first clarified the definition of carbohydrate-binding proteins and ... We also examined two amino acid grouping methods that enable effective learning of sequence patterns and evaluated the ...
more infohttps://www.hindawi.com/journals/abi/2010/289301/abs/

Prediction of Carbohydrate-Binding Proteins from Sequences Using Support Vector MachinesPrediction of Carbohydrate-Binding Proteins from Sequences Using Support Vector Machines

The sequences of carbohydrate-binding protein sequences were collected from UniProtKB [9] by using a sequence retrieval system ... The sequence homology search was applied to these sequence clusters as follows.(1)For clusters consisting of a single sequence ... sequence the query sequence is predicted as a carbohydrate-binding (noncarbohydrate-binding) protein. The AUC value for this ... we regarded a protein sequence as that of a carbohydrate-binding protein (positive) if it had a sequence identity higher than ...
more infohttps://www.hindawi.com/journals/abi/2010/289301/

Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing - MBCCMSDiscrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing - MBCCMS

Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing. October 13, ... demonstrating that this technique may prove useful for the sequencing of complex oligosaccharides. ...
more infohttps://www.mbc.manchester.ac.uk/blog/2015/10/13/flitsch-group/

Carbohydrate-binding sequences of the lectin genes in leguminous plants from the Galegeae and Hedysareae Tribes, Russian...Carbohydrate-binding sequences of the lectin genes in leguminous plants from the Galegeae and Hedysareae Tribes, Russian...

"Carbohydrate-binding sequences of the lectin genes in leguminous plants from the Galegeae and Hedysareae Tribes, Russian ... Carbohydrate-binding sequences of the lectin genes in leguminous plants from the Galegeae and Hedysareae Tribes. Carbohydrate- ... Carbohydrate-binding sequences of the lectin genes in leguminous plants from the Galegeae and Hedysareae Tribes. Chubukova, O. ... The carbohydrate-binding sequences (CBS) of the lectin genes from legume plants of the genera Astragalus Lam., Oxytropis DC., ...
more infohttps://www.deepdyve.com/lp/springer_journal/carbohydrate-binding-sequences-of-the-lectin-genes-in-leguminous-INp2BVq6Vk

Amino acid sequence similarities of carbohydrate active | Open-iAmino acid sequence similarities of carbohydrate active | Open-i

Amino acid sequence similarities of carbohydrate active enzymes identified from the muskoxen rumen metatranscriptome (using all ... pone-0020521-g005: Amino acid sequence similarities of carbohydrate active enzymes identified from the muskoxen rumen ... pone-0020521-g005: Amino acid sequence similarities of carbohydrate active enzymes identified from the muskoxen rumen ... Polyadenylated RNA (mRNA) was sequenced on the Illumina Genome Analyzer II system and 2.8 gigabases of sequences were obtained ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC3105075_pone.0020521.g005&req=4

Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2-40(T) - DOE...Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2-40(T) - DOE...

Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2-40(T). ... Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2-40(T) ... The sequences were then biochemically characterized by a team led by Jennifer Doudnas group at UC Berkeley. Read more ... Cas9 needs guide RNA to lead it to the correct sequence to snip-but not all guides are effective. Researchers created a set of ...
more infohttps://jgi.doe.gov/publication/complete-genome-sequence-of-the-complex-carbohydrate-degrading-marine-bacterium-saccharophagus-degradans-strain-2-40t/

LACTOBACILLUS ACIDOPHILUS NUCLEIC ACID SEQUENCES ENCODING CARBOHYDRATE UTILIZATION-RELATED PROTEINS AND USES THEREFOR - Patent...LACTOBACILLUS ACIDOPHILUS NUCLEIC ACID SEQUENCES ENCODING CARBOHYDRATE UTILIZATION-RELATED PROTEINS AND USES THEREFOR - Patent...

0076]Carbohydrate utilization-related nucleotide sequences identified based on their sequence identity to the carbohydrate ... As used herein, "5 leader sequence," "translation leader sequence," or "5 non-coding sequence" refer to that DNA sequence ... sequence identity to the corresponding sequence may be used. [0098]Given the coding-strand sequence encoding a carbohydrate ... Sequence Identity [0064]The carbohydrate utilization-related and multidrug transporter sequences are members of families of ...
more infohttp://www.patentsencyclopedia.com/app/20090093021

Variation of Neisseria gonorrhoeae lipooligosaccharide directs dendritic cell-induced T helper responses.Variation of Neisseria gonorrhoeae lipooligosaccharide directs dendritic cell-induced T helper responses.

A naturally occurring variation of the terminal carbohydrates on the lipooligosaccharide (LOS) molecule correlates with altered ... Carbohydrate Sequence. Cells, Cultured. Cricetinae. Cricetulus. Dendritic Cells / immunology*. Female. Glycosylation. Humans. ... The carbohydrate moiety of GC LOS is subject to phase variation, creating a GC population with different terminal carbohydrate ... Molecular Sequence Data. Neisseria gonorrhoeae / immunology*. Phenotype. T-Lymphocytes, Helper-Inducer / immunology*. ...
more infohttp://www.biomedsearch.com/nih/Variation-Neisseria-gonorrhoeae-lipooligosaccharide-directs/19834553.html

In vitro biosynthesis of glycosylphosphatidylinositol in Aspergillus fumigatus.In vitro biosynthesis of glycosylphosphatidylinositol in Aspergillus fumigatus.

Carbohydrate Sequence. Coenzyme A / metabolism. Ethanolamines / metabolism. Ethylmaleimide / chemistry. Glycolipids / ... Molecular Sequence Data. Oligopeptides / chemistry. Phosphatidylinositols / biosynthesis, chemistry. Uridine Diphosphate N- ...
more infohttp://www.biomedsearch.com/nih/In-vitro-biosynthesis-glycosylphosphatidylinositol-in/15568819.html

Reinhold VN[au] - PubMed - NCBIReinhold VN[au] - PubMed - NCBI

Congruent strategies for carbohydrate sequencing. 3. OSCAR: an algorithm for assigning oligosaccharide topology from MSn data. ... Structural characterization of carbohydrate sequence, linkage, and branching in a quadrupole Ion trap mass spectrometer: ... Detailed characterization of carbohydrate linkage and sequence in an ion trap mass spectrometer: glycosphingolipids. ... Carbohydrate structural isomers analyzed by sequential mass spectrometry.. Ashline DJ, Lapadula AJ, Liu YH, Lin M, Grace M, ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=Reinhold+VN%5Bau%5D&dispmax=50

Studies directed to understanding the structure of chitosan-metal complexes: Investigations of mono- and disaccharide models...Studies directed to understanding the structure of chitosan-metal complexes: Investigations of mono- and disaccharide models...

keywords = "Carbohydrate Sequence, Chitosan, Crystallography, X-Ray, Disaccharides, Indicators and Reagents, Magnetic Resonance ... carbohydrate analysis, chemistry, molecular genetics, nuclear magnetic resonance spectroscopy, synthesis, X ray crystallography ... PLATINUM compounds, CHEMISTRY,, SUPPORTED PALLADIUM CATALYST, CRYSTAL-STRUCTURE, CARBOHYDRATE-RECOGNITION, NITROPHENOL ... Spectroscopy, Metals, Molecular Sequence Data, Monosaccharides, Oligosaccharides, Platinum Compounds, 9012-76-4 Chitosan, ...
more infohttps://orbit.dtu.dk/en/publications/studies-directed-to-understanding-the-structure-of-chitosan-metal

A sub-population of keratan sulphates derived from bovine articular cartilage is capped with alpha(2-6)-linked N...A sub-population of keratan sulphates derived from bovine articular cartilage is capped with alpha(2-6)-linked N...

Animals ; Carbohydrate Conformation ; Carbohydrate Sequence ; Cartilage, Articular ; Cattle ; Chromatography, Affinity ; ... Keratan Sulfate ; Lectins ; Magnetic Resonance Spectroscopy ; Molecular Sequence Data ; N-Acetylneuraminic Acid ; Plant Lectins ...
more infohttp://eprints.lancs.ac.uk/22860/

Remediated Sequence 









- 1A0H: THE X-RAY CRYSTAL STRUCTURE OF PPACK-MEIZOTHROMBIN DESF1: KRINGLE/THROMBIN AND...Remediated Sequence - 1A0H: THE X-RAY CRYSTAL STRUCTURE OF PPACK-MEIZOTHROMBIN DESF1: KRINGLE/THROMBIN AND...

THE X-RAY CRYSTAL STRUCTURE OF PPACK-MEIZOTHROMBIN DESF1: KRINGLE/THROMBIN AND CARBOHYDRATE/KRINGLE/THROMBIN INTERACTIONS AND ... View Sequence & DSSP Image. Download Sequence Chain Image. Chain Info. Polymer: 1. Length: 159 residues. Chain Type: ... View Sequence & DSSP Image. Download Sequence Chain Image. Chain Info. Polymer: 2. Length: 259 residues. Chain Type: ... Sequence Display for the Entities in PDB 1A0H The graphical representation below shows this entrys sequences as reported in ...
more infohttp://www.rcsb.org/pdb/explore/remediatedSequence.do?structureId=1A0H

Introduction to glycoscience - Case Studies: Glycoscience and Biotherapeutics | CourseraIntroduction to glycoscience - Case Studies: Glycoscience and Biotherapeutics | Coursera

Glycoscience is the science and technology of carbohydrates, which are the most abundant biological molecules on Earth and make ... Human fertilization is mediated by a specific carbohydrate sequence. Its called sialyl-Lewis X. ... Carbohydrates provide the largest renewable biomass on Earth and are the economically viable and sustainable alternative to ... Carbohydrates are produced in the leaves of plants, from little more than water and ...
more infohttps://www.coursera.org/lecture/industrial-biotech/introduction-to-glycoscience-sFxC9

GlycomeDB - integration of open-access carbohydrate structure databases | BMC Bioinformatics | Full TextGlycomeDB - integration of open-access carbohydrate structure databases | BMC Bioinformatics | Full Text

GlycomeDB is a new, publicly available database for carbohydrate sequences with a unified, all-encompassing structure encoding ... More than 100000 datasets were imported, resulting in more than 33000 unique sequences now encoded in GlycomeDB using the ... Funding for further development of the Complex Carbohydrate Structure Database (CCSD or CarbBank) ceased in 1997, and since ... For each database, different encoding schemes for residues and sequence topology were designed. Therefore, it is virtually ...
more infohttps://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-9-384

GlycoRDF - Carbohydrate Format - Classes | NCBO BioPortalGlycoRDF - Carbohydrate Format - Classes | NCBO BioPortal

http://purl.jp/bio/12/glyco/glycan#carbohydrate_format. comment. Instances of this class are the carbohydrate sequence formats ...
more infohttp://bioportal.bioontology.org/ontologies/GLYCORDF?p=classes

retinoblastomas familial drug therapy 2000:2010[pubdate] *count=100 - BioMedLib™ search engineretinoblastomas familial drug therapy 2000:2010[pubdate] *count=100 - BioMedLib™ search engine

Molecular Sequence. A2.1.5.3.1. Nucleotide Sequence. A2.1.5.3.2. Amino Acid Sequence. A2.1.5.3.3. Carbohydrate Sequence. A2.1. ... Carbohydrate Conformation. Carbohydrate Sequence. Cell Line, Tumor. Cell Survival / drug effects. Cell Survival / physiology. ... Carbohydrate. A1.4.1.2.1.9. Lipid. A1.4.1.2.1.9.1. Steroid. A1.4.1.2.1.9.2. Eicosanoid. A1.4.1.2.2. Inorganic Chemical. A1.4. ... Molecular Sequence Data. Molecular Structure. Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization. Structure- ...
more infohttp://www.bmlsearch.com/?kwr=retinoblastomas+familial+drug+therapy+2000:2010%5Bpubdate%5D&cxts=100&stmp=b1

Glycoinformatics | Springer for Research & DevelopmentGlycoinformatics | Springer for Research & Development

This book provides current glycoinformatics methods and protocols used to support the determination of carbohydrate structures ... in biological samples as well as carbohydrate structure databases, the in ... and analyzing carbohydrate sequences and their taxonomy using computers. Written in the highly successful Methods in ... Branched structures Carbohydrate structure databases Carbohydrates Glycan data Glycobiology Glycolipids Glycoproteins Protein- ...
more infohttps://rd.springer.com/book/10.1007/978-1-4939-2343-4

ZFIN Publication: Chang et al., 2009ZFIN Publication: Chang et al., 2009

Carbohydrate Sequence. *Cloning, Molecular. *Embryo, Nonmammalian/enzymology. *Gene Expression Regulation, Developmental*. * ...
more infohttps://zfin.org/ZDB-PUB-080722-33
  • The N-glycan core structure has been chosen to illustrate the different encoding schemes used in the various databases for a given carbohydrate sequence. (nih.gov)
  • Finally, ion-mobility mass spectrometry following fragmentation was used to determine the nature of both the reducing and non-reducing glycans of a series of epimeric disaccharides and the branched pentasaccharide Man3 glycan, demonstrating that this technique may prove useful for the sequencing of complex oligosaccharides. (manchester.ac.uk)
  • Nevertheless, with about 50000 entries and more than 23000 different sequences, the CarbBank is still the largest repository of glycan data available. (biomedcentral.com)
  • We used support vector machines (SVMs) [ 2 ] to create a classifier to predict whether a target protein is a carbohydrate-binding protein. (hindawi.com)
  • The sequence is written from the amino-terminal end (the first amino acid) to the carboxyl-terminal end (the same sequence in which the protein is synthesized). (encyclopedia.com)
  • All properties of a protein are derived from the primary structure, the linear sequence. (encyclopedia.com)
  • New tools for carbohydrate sulfation analysis: heparan sulfate 2- O -sulfotransferase (HS2ST) is a target for small-molecule protein kinase inhibitors. (nih.gov)
  • Carbohydrates in the Protein Data Bank (PDB) form another data source of glycosciences.de. (glycosciences.de)
  • Integrative high-throughput sequencing in personalized oncology - 10th Carbohydrate Bioengineering Meeting - 12th International Symposium on the Genetics of Industrial Microorganisms - Novel consolidated bioprocess for biofuel production in E. coli - Protein Engineering: New Approaches and Applications - Tomato genome published - Use of Google algorithm to identify pancreatic cancer biomarkers. (pan.pl)
  • The graphical representation below shows this entry's sequences as reported in UniProtKB, in the sample (SEQRES), or as observed in the experiment (ATOM). (rcsb.org)
  • The biggest obstacle for data integration was the use of various sequence encoding formats by the different initiatives (Figure 2). (nih.gov)
  • This problem is especially evident for carbohydrate databases, where sequence information is spread in incompatible formats over several unconnected databases. (biomedcentral.com)
  • Instances of this class are the carbohydrate sequence formats. (bioontology.org)
  • The name "peanut agglutinin" originates from its ability to stick together (agglutinate) cells, such as neuramidase-treated erythrocytes, which have glycoproteins or glycolipids on their surface which include the Gal-β(1-3)-GalNAc carbohydrate sequence. (wikipedia.org)
  • More than 100000 datasets were imported, resulting in more than 33000 unique sequences now encoded in GlycomeDB using the universal format GlycoCT. (nih.gov)
  • GlycomeDB - integration of open-access carbohydrate structure databases. (nih.gov)
  • Encoding schemes used in various carbohydrate sequence databases. (nih.gov)
  • Here we report the results and the peculiarities of a data integration effort which aims to overcome the disadvantages inherent in the scattering of data in isolated carbohydrate databases. (biomedcentral.com)
  • An endorsement to create open access databases for analytical data of complex carbohydrates. (glycosciences.de)
  • Plant cell wall degrading enzyme modules including glycoside hydrolases, carbohydrate esterases and polysaccharide lyases were identified from over 2500 contigs. (nih.gov)
  • We also examined two amino acid grouping methods that enable effective learning of sequence patterns and evaluated the performance of these methods. (hindawi.com)
  • Polyadenylated RNA (mRNA) was sequenced on the Illumina Genome Analyzer II system and 2.8 gigabases of sequences were obtained and 59129 contigs assembled. (nih.gov)
  • The muskoxen rumen metatranscriptome demonstrates a much higher percentage of cellulase enzyme discovery and an 8.7x higher rate of total carbohydrate active enzyme discovery per gigabase of sequence than previous rumen metagenomes. (nih.gov)