Compounds that contain three methine groups. They are frequently used as cationic dyes used for differential staining of biological materials.
Axons of certain cells in the DENTATE GYRUS. They project to the polymorphic layer of the dentate gyrus and to the proximal dendrites of PYRAMIDAL CELLS of the HIPPOCAMPUS. These mossy fibers should not be confused with mossy fibers that are cerebellar afferents (see NERVE FIBERS).
GRAY MATTER situated above the GYRUS HIPPOCAMPI. It is composed of three layers. The molecular layer is continuous with the HIPPOCAMPUS in the hippocampal fissure. The granular layer consists of closely arranged spherical or oval neurons, called GRANULE CELLS, whose AXONS pass through the polymorphic layer ending on the DENDRITES of PYRAMIDAL CELLS in the hippocampus.
Specialized junctions at which a neuron communicates with a target cell. At classical synapses, a neuron's presynaptic terminal releases a chemical transmitter stored in synaptic vesicles which diffuses across a narrow synaptic cleft and activates receptors on the postsynaptic membrane of the target cell. The target may be a dendrite, cell body, or axon of another neuron, or a specialized region of a muscle or secretory cell. Neurons may also communicate via direct electrical coupling with ELECTRICAL SYNAPSES. Several other non-synaptic chemical or electric signal transmitting processes occur via extracellular mediated interactions.
The capacity of the NERVOUS SYSTEM to change its reactivity as the result of successive activations.
Depolarization of membrane potentials at the SYNAPTIC MEMBRANES of target neurons during neurotransmission. Excitatory postsynaptic potentials can singly or in summation reach the trigger threshold for ACTION POTENTIALS.
The communication from a NEURON to a target (neuron, muscle, or secretory cell) across a SYNAPSE. In chemical synaptic transmission, the presynaptic neuron releases a NEUROTRANSMITTER that diffuses across the synaptic cleft and binds to specific synaptic receptors, activating them. The activated receptors modulate specific ion channels and/or second-messenger systems in the postsynaptic cell. In electrical synaptic transmission, electrical signals are communicated as an ionic current flow across ELECTRICAL SYNAPSES.
A persistent increase in synaptic efficacy, usually induced by appropriate activation of the same synapses. The phenomenological properties of long-term potentiation suggest that it may be a cellular mechanism of learning and memory.
Congenital disorder affecting all bone marrow elements, resulting in ANEMIA; LEUKOPENIA; and THROMBOPENIA, and associated with cardiac, renal, and limb malformations as well as dermal pigmentary changes. Spontaneous CHROMOSOME BREAKAGE is a feature of this disease along with predisposition to LEUKEMIA. There are at least 7 complementation groups in Fanconi anemia: FANCA, FANCB, FANCC, FANCD1, FANCD2, FANCE, FANCF, FANCG, and FANCL. (from Online Mendelian Inheritance in Man, http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=227650, August 20, 2004)
A diverse group of proteins whose genetic MUTATIONS have been associated with the chromosomal instability syndrome FANCONI ANEMIA. Many of these proteins play important roles in protecting CELLS against OXIDATIVE STRESS.
Hemorrhagic and thrombotic disorders that occur as a consequence of inherited abnormalities in blood coagulation.
A Fanconi anemia complementation group protein that regulates the activities of CYTOCHROME P450 REDUCTASE and GLUTATHIONE S-TRANSFERASE. It is found predominately in the CYTOPLASM, but moves to the CELL NUCLEUS in response to FANCE PROTEIN.
A Fanconi anemia complementation group protein that undergoes mono-ubiquitination by FANCL PROTEIN in response to DNA DAMAGE. Also, in response to IONIZING RADIATION it can undergo PHOSPHORYLATION by ataxia telangiectasia mutated protein. Modified FANCD2 interacts with BRCA2 PROTEIN in a stable complex with CHROMATIN, and it is involved in DNA REPAIR by homologous RECOMBINATION.
A Fanconi anemia complementation group protein that is the most commonly mutated protein in FANCONI ANEMIA. It undergoes PHOSPHORYLATION by PROTEIN KINASE B and forms a complex with FANCC PROTEIN in the CELL NUCLEUS.
Pigmenting photosensitizing agent obtained from several plants, mainly Psoralea corylifolia. It is administered either topically or orally in conjunction with ultraviolet light in the treatment of vitiligo.
Colloids formed by the combination of two immiscible liquids such as oil and water. Lipid-in-water emulsions are usually liquid, like milk or lotion. Water-in-lipid emulsions tend to be creams. The formation of emulsions may be aided by amphiphatic molecules that surround one component of the system to form MICELLES.
Silver. An element with the atomic symbol Ag, atomic number 47, and atomic weight 107.87. It is a soft metal that is used medically in surgical instruments, dental prostheses, and alloys. Long-continued use of silver salts can lead to a form of poisoning known as ARGYRIA.
Derivatives of ammonium compounds, NH4+ Y-, in which all four of the hydrogens bonded to nitrogen have been replaced with hydrocarbyl groups. These are distinguished from IMINES which are RN=CR2.
Hydrocarbons with at least one triple bond in the linear portion, of the general formula Cn-H2n-2.
A family of nonmetallic, generally electronegative, elements that form group 17 (formerly group VIIa) of the periodic table.
Exclusive legal rights or privileges applied to inventions, plants, etc.
Emulsions of fats or lipids used primarily in parenteral feeding.
Inorganic compounds that contain phosphorus as an integral part of the molecule.
A broad class of substances encompassing all those that do not include carbon and its derivatives as their principal elements. However, carbides, carbonates, cyanides, cyanates, and carbon disulfide are included in this class.
Nanometer-scale wires made of materials that conduct electricity. They can be coated with molecules such as antibodies that will bind to proteins and other substances.
The testing of materials and devices, especially those used for PROSTHESES AND IMPLANTS; SUTURES; TISSUE ADHESIVES; etc., for hardness, strength, durability, safety, efficacy, and biocompatibility.
Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.
Characteristics or attributes of the outer boundaries of objects, including molecules.
An essential ribonucleoprotein reverse transcriptase that adds telomeric DNA to the ends of eukaryotic CHROMOSOMES.
Higher-order DNA and RNA structures formed from guanine-rich sequences. They are formed around a core of at least 2 stacked tetrads of hydrogen-bonded GUANINE bases. They can be formed from one two or four separate strands of DNA (or RNA) and can display a wide variety of topologies, which are a consequence of various combinations of strand direction, length, and sequence. (From Nucleic Acids Res. 2006;34(19):5402-15)
A terminal section of a chromosome which has a specialized structure and which is involved in chromosomal replication and stability. Its length is believed to be a few hundred base pairs.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.
Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles is covered with a lattice-like network of the protein CLATHRIN. Shortly after formation, however, the clathrin coat is removed and the vesicles are referred to as ENDOSOMES.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
An adaptor protein complex primarily involved in the formation of clathrin-related endocytotic vesicles (ENDOSOMES) at the CELL MEMBRANE.
The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. Each molecule of clathrin consists of three light chains (CLATHRIN LIGHT CHAINS) and three heavy chains (CLATHRIN HEAVY CHAINS) that form a structure called a triskelion. Clathrin also interacts with cytoskeletal proteins.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Specialized regions of the cell membrane composed of pits coated with a bristle covering made of the protein CLATHRIN. These pits are the entry route for macromolecules bound by cell surface receptors. The pits are then internalized into the cytoplasm to form the COATED VESICLES.
Components of a cell.
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
The palm family of order Arecales, subclass Arecidae, class Liliopsida.
Sequential operating programs and data which instruct the functioning of a digital computer.
A genus of newts of the Salamandridae family found in North America in areas east of the 100th meridian. A common species is NOTOPHTHALMUS VIRIDESCENS.
The use of molecularly targeted imaging probes to localize and/or monitor biochemical and cellular processes via various imaging modalities that include RADIONUCLIDE IMAGING; ULTRASONOGRAPHY; MAGNETIC RESONANCE IMAGING; FLUORESCENCE IMAGING; and MICROSCOPY.
Persons or entities that introduce a novel composition, device, or process, as well as improvements thereof.
Compounds that contain the triphenylmethane aniline structure found in rosaniline. Many of them have a characteristic magenta color and are used as COLORING AGENTS.
Green dyes containing ammonium and aryl sulfonate moieties that facilitate the visualization of tissues, if given intravenously. They have mostly been used in the study of kidney physiology.
A plant genus of the family CUCURBITACEAE known for the edible fruit.
Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)

17beta-estradiol reduces tumor necrosis factor-alpha-mediated LDL accumulation in the artery wall. (1/1146)

Estrogens have direct effects on the vascular wall that may prevent the development of atherosclerosis. In particular, estrogens, such as 17beta-estradiol (estradiol), are known to have potent antioxidant activity. Tumor necrosis factor-alpha (TNF) is found in human atheroma and produces oxygen-derived free radicals. These oxygen-derived free radicals may modify low density lipoproteins (LDL) and increase LDL binding in the artery wall. We asked: 1) does TNF increase LDL accumulation in the artery wall and 2) can the TNF-mediated increase in LDL accumulation be prevented by the antioxidant activity of estradiol? Carotid arteries from ovariectomized 3-month-old rats were removed and perfused with fluorescently labeled LDL and arterial LDL flux was measured using quantitative fluorescence microscopy. In six arteries, addition of TNF (10 ng/ml) to the perfusate resulted in a 2.3-fold increase in the rate of LDL accumulation (1.50 +/- 0.37 ng/min per cm2 vs. 3.38 +/- 0.48 ng/min per cm2; P < 0.01). Estradiol (65 pg/ml) and alpha-tocopherol (6 mg/L) both attenuated TNF-mediated LDL accumulation (P < 0.05), indicating that TNF may exert its effects on LDL accumulation through cellular production of oxygen-derived free radicals. These results support an antioxidant role for estradiol in the protection against LDL accumulation in the artery wall and subsequent progression of atherosclerosis.  (+info)

Development of cephalic neural crest cells in embryos of Lampetra japonica, with special reference to the evolution of the jaw. (2/1146)

Neural crest cells contribute extensively to vertebrate head morphogenesis and their origin is an important question to address in understanding the evolution of the craniate head. The distribution pattern of cephalic crest cells was examined in embryos of one of the living agnathan vertebrates, Lampetra japonica. The initial appearance of putative crest cells was observed on the dorsal aspect of the neural rod at stage 20.5 and ventral expansion of these cells was first seen at the level of rostral somites. As in gnathostomes, cephalic crest cells migrate beneath the surface ectoderm and form three major cell populations, each being separated at the levels of rhombomeres (r) 3 and r5. The neural crest seems initially to be produced at all neuraxial levels except for the rostral-most area, and cephalic crest cells are secondarily excluded from levels r3 and r5. Such a pattern of crest cell distribution prefigures the morphology of the cranial nerve anlage. The second or middle crest cell population passes medial to the otocyst, implying that the otocyst does not serve as a barrier to separate the crest cell populations. The three cephalic crest cell populations fill the pharyngeal arch ventrally, covering the pharyngeal mesoderm laterally with the rostral-most population covering the premandibular region and mandibular arch. The third cell population is equivalent to the circumpharyngeal crest cells in the chick, and its influx into the pharyngeal region precedes the formation of postotic pharyngeal arches. Focal injection of DiI revealed the existence of an anteroposterior organization in the neural crest at the neurular stage, destined for each pharyngeal region. The crest cells derived from the posterior midbrain that express the LjOtxA gene, the Otx2 cognate, were shown to migrate into the mandibular arch, a pattern which is identical to gnathostome embryos. It was concluded that the head region of the lamprey embryo shares a common set of morphological characters with gnathostome embryos and that the morphological deviation of the mandibular arch between the gnathostomes and the lamprey is not based on the early embryonic patterning.  (+info)

Characterization of nodular neuronal heterotopia in children. (3/1146)

Neuronal heterotopia are seen in various pathologies and are associated with intractable epilepsy. We examined brain tissue from four children with subcortical or periventricular nodular heterotopia of different aetiologies: one with severe epilepsy following focal brain trauma at 17 weeks gestation, one with hemimegalencephaly and intractable epilepsy, one with focal cortical dysplasia and intractable epilepsy, and one dysmorphic term infant with associated hydrocephalus and polymicrogyria. The connectivity of nodules was investigated using histological and carbocyanine dye (DiI) tracing techniques. DiI crystal placement adjacent to heterotopic nodules revealed numerous DiI-labelled fibres within a 2-3 mm radius of the crystals. Although we observed labelled fibres closely surrounding nodules, the majority did not penetrate them. Placement of DiI crystals within nodules also identified a limited number of projections out of the nodules and in one case there was evidence for connectivity between adjacent nodules. The cellular and neurochemical composition of nodules was also examined using immunohistochemistry for calretinin and neuropeptide Y (NPY), which are normally expressed in GABAergic cortical interneurons. Within heterotopic nodules from all cases, numerous calretinin-positive neurons were identified, along with a few cell bodies and many processes positive for NPY. Calretinin-positive neurons within nodules were less morphologically complex than those in the cortex, which may reflect incomplete differentiation into an inhibitory neuronal phenotype. There were also abnormal clusters of calretinin-positive cells in the overlying cortical plate, indicating that the migratory defect which produces heterotopic nodules also affects development of the cortex itself. Thus, heterotopic nodules consisting of multiple neuronal cell types are associated with malformation in the overlying cortical plate, and have limited connectivity with other brain regions. This abnormal development of connectivity may affect neuronal maturation and consequently the balance of excitation and inhibition in neuronal circuits, leading to their epileptogenic potential.  (+info)

Identification of megalin/gp330 as a receptor for lipoprotein(a) in vitro. (4/1146)

Lipoprotein(a) [Lp(a)] is an atherogenic lipoprotein of unknown physiological function. The mechanism of Lp(a) atherogenicity as well as its catabolic pathways are only incompletely understood at present. In this report, we show that the low density lipoprotein receptor (LDLR) gene family member megalin/glycoprotein (gp) 330 is capable of binding and mediating the cellular uptake and degradation of Lp(a) in vitro. A mouse embryonic yolk sac cell line with native expression of megalin/gp330 but genetically deficient in LDLR-related protein (LRP) and a control cell line carrying a double knockout for both LRP and megalin/gp330 were compared with regard to their ability to bind, internalize, and degrade dioctadecyltetramethylindocarbocyanine perchlorate (DiI)-fluorescence-labeled Lp(a) as well as equimolar amounts of 125I-labeled Lp(a) and LDL. Uptake and degradation of radiolabeled Lp(a) by the megalin/gp330-expressing cells were, on average, 2-fold higher than that of control cells. This difference could be completely abolished by addition of the receptor-associated protein, an inhibitor of ligand binding to megalin/gp330. Mutual suppression of the uptake of 125I-Lp(a) and of 125I-LDL by both unlabeled Lp(a) and LDL suggested that Lp(a) uptake is mediated at least partially by apolipoprotein B100. Binding and uptake of DiI-Lp(a) resulted in strong signals on megalin/gp330-expressing cells versus background only on control cells. In addition, we show that purified megalin/gp330, immobilized on a sensor chip, directly binds Lp(a) in a Ca2+-dependent manner with an affinity similar to that for LDL. We conclude that megalin/gp330 binds Lp(a) in vitro and is capable of mediating its cellular uptake and degradation.  (+info)

Endocytic sorting of lipid analogues differing solely in the chemistry of their hydrophobic tails. (5/1146)

To understand the mechanisms for endocytic sorting of lipids, we investigated the trafficking of three lipid-mimetic dialkylindocarbocyanine (DiI) derivatives, DiIC16(3) (1,1'-dihexadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), DiIC12(3) (1,1'- didodecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), and FAST DiI (1,1'-dilinoleyl-3,3,3', 3'-tetramethylindocarbocyanine perchlorate), in CHO cells by quantitative fluorescence microscopy. All three DiIs have the same head group, but differ in their alkyl tail length or unsaturation; these differences are expected to affect their distribution in membrane domains of varying fluidity or curvature. All three DiIs initially enter sorting endosomes containing endocytosed transferrin. DiIC16(3), with two long 16-carbon saturated tails is then delivered to late endosomes, whereas FAST DiI, with two cis double bonds in each tail, and DiIC12(3), with saturated but shorter (12-carbon) tails, are mainly found in the endocytic recycling compartment. We also find that DiOC16(3) (3,3'- dihexadecyloxacarbocyanine perchlorate) and FAST DiO (3, 3'-dilinoleyloxacarbocyanine perchlorate) behave similarly to their DiI counterparts. Furthermore, whereas a phosphatidylcholine analogue with a BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) fluorophore attached at the end of a 5-carbon acyl chain is delivered efficiently to the endocytic recycling compartment, a significant fraction of another derivative with BODIPY attached to a 12-carbon acyl chain entered late endosomes. Our results thus suggest that endocytic organelles can sort membrane components efficiently based on their preference for association with domains of varying characteristics.  (+info)

Reactive oxygen metabolites increase mitochondrial calcium in endothelial cells: implication of the Ca2+/Na+ exchanger. (6/1146)

In endothelial cells, a bolus of hydrogen peroxide (H2O2) or oxygen metabolites generated by hypoxanthine-xanthine oxidase (HX-XO) increased the mitochondrial calcium concentration [Ca2+]m. Both agents caused a biphasic increase in [Ca2+]m which was preceded by a rise in cytosolic free calcium concentration [Ca2+]c (18 and 6 seconds for H2O2 and HX-XO, respectively). The peak and plateau elevations of [Ca2+] were consistently higher in the mitochondrial matrix than in the cytosol. In Ca2+-free/EGTA medium, the plateau phase of elevated [Ca2+] evoked by H2O2 due to capacitative Ca2+ influx was abolished in the cytosol, but was maintained in the mitochondria. In contrast to H2O2 and HX-XO, ATP which binds the P2Y purinoceptors induced an increase in [Ca2+]m that was similar to that of [Ca2+]c. When cells were first stimulated with inositol 1,4, 5-trisphosphate-generating agonists or the Ca2+-ATPase inhibitor cyclopiazonic acid (CPA), subsequent addition of H2O2 did not affect [Ca2+]c, but still caused an elevation of [Ca2+]m. Moreover, the specific inhibitor of the mitochondrial Ca2+/Na+ exchanger, 7-chloro-3,5-dihydro-5-phenyl-1H-4.1-benzothiazepine-2-on (CGP37157), did not potentiate the effects of H2O2 and HX-XO on [Ca2+]m, while causing a marked increase in the peak [Ca2+]m and a significant attenuation of the rate of [Ca2+]m efflux upon addition of histamine or CPA. In permeabilized cells, H2O2 mimicked the effects of CGP37157 causing an increase in the basal level of matrix free Ca2+ and decreased efflux. Dissipation of the electrochemical proton gradient by carbonylcyanide p-(trifluoromethoxy) phenylhydrazone (FCCP), and blocade of the Ca2+ uptake by ruthenium red prevented [Ca2+]m increases evoked by H2O2. These results demonstrate that the H2O2-induced elevation in [Ca2+]m results from a transfer of Ca2+ secondary to increased [Ca2+]c, and an inhibition of the Ca2+/Na+ electroneutral exchanger of the mitochondria.  (+info)

Optical mapping of neural network activity in chick spinal cord at an intermediate stage of embryonic development. (7/1146)

We have applied multiple-site optical recording of transmembrane potential changes to recording of neuronal pathway/network activity from embryonic chick spinal cord slice preparations. Spinal cord preparations were dissected from 8-day-old chick embryos at Hamburger-Hamilton stage 33, and transverse slice preparations were prepared with the 13th cervical spinal nerve or with the 2nd or 5th lumbosacral spinal nerve intact. The slice preparations were stained with a voltage-sensitive merocyanine-rhodanine dye (NK2761). Transmembrane voltage-related optical (dye-absorbance) changes evoked by spinal nerve stimulation with positive square-current pulses using a suction electrode were recorded simultaneously from many loci in the preparation, using a 128- or 1,020-element photodiode array. Optical responses were detected from dorsal and ventral regions corresponding to the posterior (dorsal) and anterior (ventral) gray horns. The optical signals were composed of two components, fast spike-like and slow signals. In the dorsal region, the fast spike-like signal was identified as the presynaptic action potential in the sensory nerve and the slow signal as the postsynaptic potential. In the ventral region, the fast spike-like signal reflects the antidromic action potential in motoneurons, and the slow signal is related to the postsynaptic potential evoked in the motoneuron. In preparations in which the ventral root was cut microsurgically, the antidromic action potential-related optical signals were eliminated. The areas of the maximal amplitude of the evoked signals in the dorsal and ventral regions were located near the dorsal root entry zone and the ventral root outlet zone, respectively. Quasiconcentric contour-line maps were obtained in the dorsal and ventral regions, suggesting the functional arrangement of the dorsal and ventral synaptic connections. Synaptic fatigue induced by repetitive stimuli in the ventral synapses was more rapid than in the dorsal synapses. The distribution patterns of the signals were essentially similar among C13, LS2, and LS5 preparations, suggesting that there is no difference in the spatiotemporal pattern of the neural responses along the rostrocaudal axis of the spinal cord at this developmental stage. In the ventral root-cut preparations, comparing the delay times between the ventral slow optical signals, we have been able to demonstrate that neural network-related synaptic connections are generated functionally in the embryonic spinal cord at Hamburger-Hamilton stage 33.  (+info)

Correct targeting of dihydropyridine receptors and triadin in dyspedic mouse skeletal muscle in vivo. (8/1146)

Excitation-contraction coupling in skeletal muscle involves junctions (triads and dyads) between sarcoplasmic reticulum (SR) and transverse (T) -tubules. Two proteins of the junctional SR, ryanodine receptors (RyRs) and triadin and one protein of T tubules, dihydropyridine receptors (DHPRs) are located at these junctions. We studied the targeting of DHPRs and triadin to T-tubules and SR in skeletal muscles of dyspedic mouse embryos lacking RyR1. In normal differentiating muscle fibers DHPRs, triadin and RyRs are located in intensely immunolabeled foci that are randomly distributed across the fiber. Correlation with electron microscopy and with previous studies indicates that the foci represent the location of triads and dyads. In dyspedic fibers DHPRs and triadin antibodies stain internal foci of the two proteins; RyR antibodies are completely negative. The appearance and location of the foci in dyspedic fibers is similar to that of normal muscle, but their fluorescent intensity is weaker. The SR Ca-ATPase has more diffuse distribution than triadin in both normal and dyspedic fibers. These observations indicate that an interaction with RyRs is not necessary for the appropriate targeting of DHPRs or triadin to junctional domains of T tubules and SR respectively.  (+info)

Spectral properties of carbocyanine dye 3-methyl-2-[3-methyl-2-(3-methyl-2,3-dihydro-1,3-benzothiazole-2-iliden)-1- butenyl]-1,3-benzothiazole-3-il iodide (Cyan betaiPr) in water solution, as well as in the presence of different types of double stranded DNA have been studied. While in water solution of free dye Cyan betaiPr stays mainly in monomeric form, in the presence of DNA the dye molecules form J-aggregates. The molecular structure of these J-aggregates causes the Davydov splitting of their absorption band, corresponding to the first electronic transition. A study of site-specificity showed that in the presence of poly (dA/dT) the majority of Cyan betaiPr molecules form J-aggregates, while in the presence of poly (dGC/dGC) dye molecules stay mainly in monomeric form and in presence of chicken erythrocytes DNA both J-aggregate and monomeric forms of dye are present. We suppose that Cyan betaiPr molecules aggregate in DNA groove, which serves as a template for J-aggregate forming. An ...
We have examined the migration of murine macrophages from the vascular compartment to normal and inflammatory tissues by the adoptive transfer of resident peritoneal macrophages (RPM phi) fluorescently labeled with the hydrophobic dye 1,1-dioctadecyl 3,3,3,3-tetramethylindocarbocyanine perchlorate (DiI). After initial labeling of the plasma membrane of RPM phi, the dye accumulated stably in intracellular vesicles of low density (rho = 1.042-1.045 kg/l) and cells remained viable in culture for 4 weeks. Like the normal monocyte, DiI-RPM phi, but not exudate-derived or fixed cells, migrated to peritoneal exudates, following i.v. adoptive transfer, by a mechanism inhibitable by an antibody to the type 3 complement receptor. In the absence of an inflammatory stimulus there was no migration to the peritoneal cavity, and DiI-RPM phi accumulated within 4 h in the red pulp and marginal zone of the spleen. By day 6 these cells still formed a tight ring of fluorescence in the marginal zone alone, ...
Link Technologies solid-supported Cyanine 540 and 650 dyes increase workflow efficiency Link Technologies Ltd, an ISO 9001:2008 certified specialist oligonucleotide reagent manufacturer, has launched a range of high quality cyanine dye products to support the continued increase in demand for fluorescent labelling in biological imaging and molecular diagnostics. As well as introducing a new range of 3-CPG solid supports modified with Cyanine 540 and Cyanine 650, Link Technologies now also manufactures and supplies the commonly used phosphoramidites, so researchers can now source an extended range of cyanine reagents produced to Link Technologies exacting standards...
We prepared silica NPs doped with two far-red absorption cyanine dyes, FR670 and Cy5, using the microemulsion method. At these wavelengths there is less autofluorescence from biological molecules, solvents and substrates. The effects of changing surfactant, pH and dye loading on NP morphology and colloidal stability were investigated using transmission electron microscopy and photo correlation spectroscopy. We found that the incorporation of Cy5 and FR670 was only possible using negatively charged surfactants. We propose a method for the facile incorporation of cyanine dye based on the electrostatic repulsion between negatively charged dye molecules and the negatively charged surfactant, present at the water oil interface, which drives the dye into the silica NP forming inside the water phase. The absorption, fluorescence and quantum efficiency of the NPs were also measured and compared against free dye labels. The optimal fluorescences for FR670 and Cy5 dye doped NPs, were 178 and 83 times brighter
Cyanine is the non-systematic name of a synthetic dye family belonging to polymethine group. The word cyanin is from the English word cyan, which conventionally means a shade of blue-green (close to aqua) and is derived from the Greek κυάνεος/κυανοῦς kyaneos/kyanous which means a somewhat different color: dark blue. Cyanines were and are still used in industry, and more recently in biotechnology (labeling, analysis). Cyanines have many uses as fluorescent dyes, particularly in biomedical imaging. Depending on the structure, they cover the spectrum from IR to UV. There are a large number reported in the literature. There are three types of cyanines: Streptocyanines or open chain cyanines: R2N+=CH[CH=CH]n-NR2 (I) Hemicyanines: Aryl=N+=CH[CH=CH]n-NR2 (II) Closed chain cyanines: Aryl=N+=CH[CH=CH]n-N=Aryl (III) where two nitrogens are joined by a polymethine chain. Both nitrogens are each independently part of a heteroaromatic moiety, such as pyrrole, imidazole, thiazole, ...
We have used carbocyanine dyes (DiI and DiO) to generate fate maps for the epiblast layer of the chick embryo between stage X and the early primitive streak stage (stages 2-3). The overall distribution of presumptive cell types in these maps is similar to that described for other laboratory species …
DNA microarray technology is a high throughput technique by which the expression of the whole genome is studied in a single experiment. In dual label experiments the fluorescent dyes Cy 3 and Cy 5 are used to label the two RNA samples co-hybridized on a same array. Recently two more dyes have been proposed (Alexa 488 and Alexa 594) allowing the simultaneous hybridization of three or four samples. Forster et al. [2] have evaluated triple-target microarray by comparing results of single-target, dual-target and triple-target microarrays. They have concluded that the use of triple-target microarray is valid from an experimental point of view. One year later, Staal et al. [7] have investigated the four-target microarray experiments. Their approach differs from that of [2], but their conclusions are in fair agreement. Their study has shown that Alexa 594 is best suited as a third dye and that Alexa 488 can be applied as a fourth dye on some microarray types. These extensions of the microarray ...
Woolery G.L.; Powers L.; Winkler M.; Solomon E.I.; Spiro T.G., 1984: Extended x ray absorption fine structure studies of bi nuclear copper site of oxy hemo cyanin deoxy hemo cyanin metaquo hemo cyanin met fluoro hemo cyanin and met azido hemo cyanin from arthropods and mollusks
This section illustrates a culture of HeLa cells that were labeled with the lipophilic cell tracer carbocyanine dye, Dil, which targets membranes. The absorption maximum of Dil is 549 nanometers and the emission maximum occurs at 565 nanometers (in the yellow region of the visible light spectrum). Fluorescence intensity arising from labeled cytoskeletal membrane components is quite evident in the image even through the probe emission peak is 30 nanometers below the Y-2E/C filter set dichromatic mirror cut-on wavelength.
S. B. Anantharaman et al., Strongly Red-Shifted Photoluminescence Band Induced by Molecular Twisting in Cyanine (Cy3) Dye Films, The Journal of Physical Chemistry C, 2017, DOI: 10.1021/acs.jpcc.7b01412 (open access). http://pubs.acs.org/doi/suppl/10.1021/acs.jpcc.7b01412. Besides in silver halide photography, industrial applications of dye aggregates are hampered due to difficulties in producing aggregates reproducibly, with controlled size and perfection and avoiding complex manufacturing processes such as Langmuir-Blodgett techniques.. While cyanine dye aggregates are best known for intense and narrow absorption bands, our films stick out for prominent scattering in the absorption band (resonance light scattering). Similar to localized surface plasmon resonances (LSPR), scattering can be explained with the coherently oscillating electron cloud of the aggregate in response to incident light. Because dye aggregate extinction has narrowest bandwidths, the wavelength selectivity exceeds the ...
Both methylmercury (MeHg) and inorganic divalent mercury (Hg++) alter the flux of ions and small molecules across nerve terminal membranes by mechanisms that may involve membrane depolarization. We compared the effects of MeHg and Hg++ on plasma (psi p) and mitochondrial membrane potentials (psi m) in synaptosomes using the potentiometric carbocyanine dye 3,3-diethylthiadicarbocyanine iodide [diS-C2(5)]. Both mercurials (1-20 microM) produced concentration-dependent increases in dye fluorescence after 5 min of exposure which were not altered by removal of Ca++ from the medium. To determine directly effects of mercurials on psi p, predepolarization of psi m using NaN3 and oligomycin was necessary. Under this condition, MeHg- and Hg(++)-induced increases in fluorescence were associated with depolarization of psi p. A second approach was used to assess changes in psi p. In synaptosomes, the magnitude of the increase in fluorescence resulting from depolarization of psi p with a stimulus of constant ...
The phosphorylation state of human and bovine spinal cord neurofilaments (NF) was studied by direct phosphate analysis and carbocyanine dye (Stains-all) binding to NF polypeptides resolved on SDS-polyacrylamide gels. Electrophoretically purified NF-H (200 kDa), NF-M (160 kDa), and NF-L (68 kDa) of human origin contained 24, 18, and 4 mol phosphate/mol protein, whereas bovine NF contained 53, 23, and 5 mol phosphate/mol protein, respectively. Incubation of NF preparations with E. coli alkaline phosphatase removed about 55% of the phosphate from NF-H, about 30% of the phosphate from both human and bovine NF-M, but did not change the phosphate content of NF-L. This treatment also inhibited or substantially reduced the binding of electroblotted NF-H and NF-M to 2 anti-NF monoclonal antibodies known to recognize phosphorylated sites on projection side arms. Stains-all was found to be a very sensitive probe for detection of phosphorylated cytoskeletal proteins. Without the phosphatase treatment, ...
CAS NO:3564-18-9; Chemical name:Chromoxane Cyanine R ; physical and chemical property of 3564-18-9, Chromoxane Cyanine R is provided by ChemNet.com
Cyanine fluorophores are encoded as non-canonical amino acids to produce functional proteins in cell-free translation systems and live cells for single-molecule imaging.
Methods of tracking cells in vivo and for determining in vivo cell lifetimes. Cells are labelled with cyanine dyes and detection is by measuring fluorescence, absorbance, or by detecting nuclear magnetic resonance probes included in the cyanine dyes. Using the invented methods, for example, red blood cell and platelet lifetimes are determined. Also, cells are tracked to determine sites of primary or metastatic tumors, or sites of occult infection. Further, rates at which cells pass through vessels is used to determine blood vessel patency and platelet aggregation.
Antibodies. Monoclonal antibodies specific for synaptophysin, microtubule-associated protein 2 (MAP-2), actin, and GABAA were purchased from Boehringer Mannheim (Indianapolis, IN); those for agrin (m247 and m33) were from StressGen Biotechnologies (Victoria, British Columbia, Canada). Antibodies specific for neuron-specific enolase, synapsin-I, glutamic acid decarboxylase, postsynaptic density 95 (PSD-95), and CREB were purchased from Polysciences (Warrington, PA), Molecular Probes (Eugene, OR), Chemicon (Temecula, CA), Affinity Bioreagents (Golden, CO), and New England Biolabs (Beverly, MA), respectively. Antibodies against NR1 were a kind gift from Dr. R. Huganir (Johns Hopkins University, Baltimore, MD). A polyclonal serum specific for GluR1 was raised as described (Molnar et al., 1994). Secondary antibodies conjugated with indocarbocyanine, FITC, and Texas Red were purchased from Jackson ImmunoResearch (West Grove, PA).. Oligonucleotides. Oligonucleotides were synthesized by Oligos Etc. ...
TSA® Plus Cyanine 3.5 (Cy3.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3.5 in the localized area of the probe or antibody.
TSA® Plus Cyanine 5 (Cy5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy5 in the localized area of the probe or antibody. ...
PerCP/Cyanine5.5 anti-human CD71 Antibody - CD71 is a 95 kD type II homodimeric transmembrane glycoprotein also known as T9 and transferrin receptor.
Molecular beacons were postulated to be a good fit for our design, as their complementary design to segments of the amplified DNA would allow for easy fluorescent quantification. Molecular beacons in our assays were purchased from IDT ($660 for 1580 samples ). They consist of a stem-loop structure with one end attached to a fluorophore (5′ 6 FAM) and the opposite end attached to a quencher molecule (3′ IOWA BLACK FQ). When the stem-loop structure is closed, the fluorophore is in contact with and quenched by the quencher molecule, and fluorescence is inhibited. However, when the stem-loop opens up upon binding to the amplified DNA oligomers, the quencher and fluorophore are no longer in contact and thus allows for the detection of fluorescence. On the other hand, SYBR Gold (ThermoScientific; $120 for 50,000 samples) is a fluorescent nucleic acid intercalator (cyanine dye) that stains and allows fluorescence detection of ssDNA, dsDNA, and RNA with signal enhancement of greater than 1000 fold. ...
XenoLight DiR lipophilic, NIR fluorescent cyanine dye ideal for staining cytoplasmic membrane. Ideal for in vivo imaging of T-cell or stem cell homing.
XenoLight DiR lipophilic, NIR fluorescent cyanine dye ideal for staining cytoplasmic membrane. Ideal for in vivo imaging of T-cell or stem cell homing.
Dilämmastikoksiid Elma Messer is a medicine available in a number of countries worldwide. A list of US medications equivalent to Dilämmastikoksiid Elma Messer is available on the Drugs.com website.
Tsídii Deílʼésígíí éí Ciconiiformes daolyé, ndaʼałkaahíkʼehjí; díí saadígíí éí Tsídii bidaanézí Ndahalinígíí óolyé.. Táłtłʼááh álééh dóó toohjįʼ ndiigaii dóó yáhashjool éí Ciconiiformes-jįʼ atah daasdzoh. Łaʼ ndaʼałkaahí ádaaníigo tsídiidaatsohí éí ałdóʼ díí tsídii dahyikahjįʼ atah yisdzoh daaní; náánáłaʼ ndaʼałkaahí éí doo bił ákódaatʼée da.. Díí tsídii éí tʼáá ałtso bijáád nineez, áádóó éí tábąąhgi naakai łeh; łaʼ éí halgaiigi dóó tłʼoh dahólǫ́ǫgi naakai ałdóʼ. Nááná éí bidaaʼ nineez łeh. Łaʼ éí łóóʼ dóó naʼashǫ́ʼii haalzhéehgo deílʼéés, náánáłaʼ éí tʼóó chʼosh deildeeł.. ...
http://www.fuzionproductions.com/after-madhubala-it-is-do-dil-ek-jaan-for-nautanki-films-the-same-creative-team-again-stirring-up-another-love-story-in-the-beautiful-valley-of-kashmir/ ...Rima...2013-06-05 16:46:48 | 3616695 | Do Dil Ek Jaan Forum
British Council T rkiye, d nyan n en ok tercih edilen ngilizce dil s nav IELTS (Uluslararas ngilizce Dil Yeterlilik S nav ) uzman IELTS Simon i
Kuch Ehsason Ke Saye Dil Ko Chu Jate Hain, Kuch Manzar Dil Mein Utar Jate Hain, Bejan Gulshan Mein Bhi Phool Khil Jate Hain, Jab Zindagi Mein Aap Jaise Dost Mil Jate Hain ...
Uptake of CSC-EVs by MSCsCSC-EVs stained with Vybrant Dil (in red) were incubated for 6, 24, 48 and 72 hours with MSCs. Cytoplasm staining of MSCs was obtained
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7. 厚膜集成电路汽车用喊话机、1205公安部用厚膜集成电路对讲机、20W大功率厚膜集成电路电影放映机还音设备、厚膜电阻网络、CY2A环氧包封电容器、CBB-12系列组合膜电容器、CBB-15系列组合膜电容器等6项新产品分别获南京市科委、南京电子元件公司、南京无线电公司优秀新产品奖和新产品设计奖 (1977-1984年)(负责组织、实施及参与) ...
Fomina M. V., Nikiforov A. S., Gromov S. P. Modern approaches to the synthesis and prospects for the use of cyanine dyes containing functional groups in the n-substituents // Russian Chemical Reviews. - 2016. - Vol. 85, no. 7. - P. 684-699. Methods for the synthesis of mono-, tri-, penta- and heptamethine cyanine dyes containing functional groups in N-substituents are considered. Approaches suitable for the preparation of both symmetrical and unsymmetrical cyanine dyes are presented. Examples of the practical use of this type of cyanine dyes as fluorescent labels and probes in biology and medicine, as well as of components of photoactive supramolecular structures, photosensitizers and photo- and electroluminescent materials are given. The bibliography includes 106 references. [ DOI ...
BACKGROUND: Burkitt lymphoma is a fast-growing mature B cell malignancy, whose genetic hallmark is translocation and activation of the c-myc gene. Prompt multiagent immunochemotherapy regimens can have favorable outcomes, but prognosis is poor in refractory or relapsed disease. We previously identified a novel family of near-infrared heptamethine carbocyanine fluorescent dyes (HMCD or DZ) with tumor-homing properties via organic anion-transporting peptides. These membrane carriers have uptake in tumor cells but not normal cells in cell culture, mouse and dog tumor models, patient-derived xenografts, and perfused kidney cancers in human patients. METHODS: Here we report the cytotoxic effects of a synthesized conjugate of DZ with cisplatin (CIS) on B cell lymphoma CA46, Daudi, Namalwa, Raji, and Ramos cell lines in cell culture and in xenograft tumor formation. Impaired mitochondrial membrane permeability was examined as the mechanism of DZ-CIS-induced lymphoma cell death. RESULTS: The new ...
Accumulating evidence is revealing the essential role of immune system in cancer treatment. Certain chemotherapeutic drugs can potently induce the release of cell death associated molecular patterns (CDAMPs), which accompanies cancer cell demise. CDAMPs can engage corresponding receptors on immune cells and stimulate immune responses to achieve long-term tumor control (Ma et al., 2013; Ma et al., 2014; Yang et al., 2015). Among reported CDAMPs, calreticulin (CALR), ATP and HMGB1 are well known for their immune-stimulatory effect. Here we describe the assays that we applied to measure cell death and these CDAMPs. Briefly, cell death can be analyzed by co-staining of 4,6-diamidino-2-phenylindole (DAPI) with 3,3-Dihexyloxacarbocyanine Iodide [DiOC6(3)] or Annexin V. CALR exposure on the cell membrane can be detected by flow cytometry. ATP and HMGB1 release can be quantified by luminescence assay and ELISA assay respectively.
The green fluorescent, lipophilic carbocyanine DiOC18(3) is widely used as a lipophilic tracer.,,The green fluorescent, lipophilic carbocyanine DiOC18(3) is widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.
The earliest generated cells of the mammalian cerebral cortex form the preplate layer (PPL). The subsequently born cortical plate (CP) cells split this layer into the superficial layer I (LI) and the deep subplate (SP). The cellular and molecular mechanisms that underlie this event are unclear. To investigate the role of the cyclin-dependent kinase 5 (Cdk5) and its activator p35 in preplate splitting, we used Nissl staining, carbocyanine dye tracing, cell birthdating, and immunohistochemistry for calretinin (CalR) in p35 and Cdk5 knockout mice. Our data demonstrated changes in early cortical lamination and aberrant thalamic axon trajectories in these mice. Specifically, LI was thicker, and cell-dense and thalamic axons did not accumulate in the SP layer before invading the CP. Instead, they grew past the SP and more superficial cortical layers and coursed obliquely toward the pial surface. This behavior has been previously observed in reeler mice and suggests a defect in PPL splitting. CalR
Cy3 and Cy5 are registered trademarks of Amersham Pharmacia Biotech Inc, Alexa Fluor is a registered trademark of Molecular Probes Inc ...
Ganglion cells in an isolated wholemount preparation of the rat retina were labeled using the DiOlistic labeling method (Gan et al., 2000) and were classified according to their morphological properties. Tungsten particles coated with a lipophilic dye (DiI) were propelled into the wholemount retin …
ChemCyte specializes in the modification of nucleosides and nucleotides. In conjunction with its catalog products, ChemCyte offers a wide range of premier custom products and research services including synthesis of nucleotide analogs, conjugation, labeling, and assay development for the life science, diagnostic and discovery research.. ...
Page contains details about Fab-conjugated GGLG/chol/PEG-DSPE/Mal-PEG-Glu2C18/DiIC18(7) liposomes . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
This invention relates to a dye donor element for laser-induced thermal dye transfer comprising a support having thereon a dye layer comprising an image dye in a polymeric binder and a cyanine infrared absorbing dye associated therewith, and wherein said layer also has a nitrosonaphthol ferrous complex associated therewith.
Our PE/Cyanine5 Anti-Human IgM Antibody[MHM-88] E-AB-F1172G flow cytometry validated antibodies offer multiple dye choices and a wide range of both intracellular and extracellular targets.
APC/Cyanine7 anti-human CD62L Antibody - CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1.
Cytoskeleton Inc provides live cell Spirochrome fluorescent Flipper-TR, tension, STED, SIM, widefield, tirf, flim, confocal, using CellMask, Cellvue, Cellbrite, Membrite, Cy5, YFP, GFP, lipophilic cyanine dyes to cell motility, phagocytosis endocytosis.
Congratulations to Dr. D. Lansing Taylor for his appointment to Distinguished Professor.. Dr. Taylor has been with the department since his return to academia in 2010. Prior to that, he held academic appointments at Harvard University and Carnegie Mellon University.. During his time as CMU, he and Dr. Alan Waggoner co-found Biological Detection Systems (BDS) to commercialize the multi-color cyanine dyes and research imaging platform. The company was later acquired by Amersham (now GE Life Sciences). He left CMU in 1997 to found Cellomics, Inc., which developed High Content Screening and later became a part of ThermoFisher Scientific. He then moved on to found Cellumen, which developed a predictive safety assessment platform using primary hepatocytes, multiplexed panels of reagents, reference safety databases and computational biology. He was CEO of Cellumen from 2004 until 2010 when it became part of Cyprotex, a British CRO. He also co-found Cernostics, Inc., a fluorescence-based, tissue systems ...
Near infrared fluorophore useful for non-invasive in vivo imaging. Absorption properties are similar to indocyanine green (ICG), but possesses higher fluorescence quantum yield.
Bernd Fritzsch is the author of this article in the Journal of Visualized Experiments: Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology
Tsídii éí tʼáá ałtso bijáád naakigo hólǫ́, áádóó éí bitʼaʼ dahólǫ́; łaʼ tsídii éí doo ndaatʼáa da ndi, tʼóó niʼgóó naakai łeh (tsídiitsoh da).. Tsídiitsoh éí yéego ntsaa (náhástʼéigo da adéesʼeez[?] éí áníłtso), dahiitį́hii łaʼ éí tʼáá díkwíí inch tʼéiyá ádaníłnééz. Tsídii éí tʼáá ałtso bidaaʼ ntłʼiz (dóó nineez łeh); áádóó éí biwooʼ ádaadin. Nááná éí bitsʼin biiʼ haltsʼaaʼ łeh.. ...
電源輸入、限流電阻、外接驅動穩壓、喇叭輸出端的端子台都排排站好在圖中的下方 ,,. JC-3正負電源輸入的點在PCB下方,正電源接到CN5、負電源接到CN6、濾波電容中點(GND)接到CN3、CN4其中一個。接好正負電源之後記得將JP1和JP2分別短路,因為JP1和JP2是預留給驅動穩壓使用的,一般情況直接短路就可以了。. 上電調整測試說明 -上電調整輸入級偏流. 接好正負電源之後,在上電之前,保險起見,分別將JP3、JP4的兩點之間串上套件內附10 Ohm /0.5W的限流電阻充當保險絲用,。. 接下來將SVR3逆時針轉到底(轉到底時會發出喀喀的聲音)將偏流調到最小,然後上電,如果發現兩顆10 ...
Abhishek Bajaj, known for his character Rahul Shashtri in TV show Dil Deke Dekho, took Jindal for a ride around Mumbai before popping up the question.
We have examined the migration of murine macrophages from the vascular compartment to normal and inflammatory tissues by the adoptive transfer of resident peritoneal macrophages (RPM phi) fluorescently labeled with the hydrophobic dye 1,1-dioctadecyl 3,3,3,3-tetramethylindocarbocyanine perchlorate (DiI). After initial labeling of the plasma membrane of RPM phi, the dye accumulated stably in intracellular vesicles of low density (rho = 1.042-1.045 kg/l) and cells remained viable in culture for 4 weeks. Like the normal monocyte, DiI-RPM phi, but not exudate-derived or fixed cells, migrated to peritoneal exudates, following i.v. adoptive transfer, by a mechanism inhibitable by an antibody to the type 3 complement receptor. In the absence of an inflammatory stimulus there was no migration to the peritoneal cavity, and DiI-RPM phi accumulated within 4 h in the red pulp and marginal zone of the spleen. By day 6 these cells still formed a tight ring of fluorescence in the marginal zone alone, ...
Carbocyanines DiI, DiS and DiO Derivatives. Indo- (DiI), thia- (DiS) and oxa- (DiO) carbocyanines with short alkyl tails (,7 ... In contrast to cationic carbocyanines, anionic bis-oxonols are largely excluded from mitochondria and are primarily sensitive ...
Carbocyanines * Receptors, N-Methyl-D-Aspartate Grant support * NS38580/NS/NINDS NIH HHS/United States ...
Carbocyanines / metabolism * Cell Line * Fanconi Anemia / metabolism* * Fanconi Anemia / pathology* * Fanconi Anemia ...
Typical slow-response probes include cationic carbocyanines and rhodamines, and ionic oxonols. Commonly used voltage sensitive ...
Effective red sensitizers are the carbocyanines of formula (IX) ##STR20## wherein. each of Z1 and Z2 represents the atoms ... Effective green sensitizers are carbocyanines and cyanines of formulae (X) and (XI) ##STR21## wherein ...
Solved in 1919-1921 the problem of the constitutions of the photographic sensitisers known as the isocyanines and carbocyanines ...
Temperature dependence of fluorescence and photoisomerization in symmetric carbocyanines. Influence of medium viscosity and ...
Suitable charge generating materials include thiopyrylium, phthalocyanines, azo pigments, perylenes, carbocyanines, squaraine, ...
Rotational Relaxation of Carbocyanines - Comparative-Study with the Isomerization Dynamics. JOURNAL OF PHYSICAL CHEMISTRY; Año ... Temperature-Dependence of Fluorescence and Photoisomerization in Symmetrical Carbocyanines - Influence of Medium Viscosity and ... AM1 study of the ground and excited state potential energy surfaces of symmetric carbocyanines ...
In this regard, several perylene compounds and carbocyanines have been shown to interact with G-quadruplex structures. Since ...
Carbocyanines are among the most strongly light-absorbing dyes known and have proven to be useful tools in several different ... Carbocyanines with short alkyl tails attached to the imine nitrogens are employed both as membrane-potential sensors (Slow- ... Like the lipophilic carbocyanines, DiA is commonly used for neuronal membrane tracing (Tracers for Membrane Labeling-Section ... Lipophilic carbocyanines have been used to visualize membrane fusion and cell permeabilization that occurs in response to ...
Multi-colour direct STORM with red emitting carbocyanines. Biol. Cell 104, 229-237 (2012).. ...
carbocyanines and carbocyanine derivatives, e.g., phenylcarbocyanine and hydrox, carbocyanines;. pyridinium salts, e.g., 4-(4- ...
Multi-colour direct STORM with red emitting carbocyanines. Biology of the Cell [http://dx.doi.org/10.1111/boc.201100011 ...
Multi-colour direct STORM with red emitting carbocyanines. Biology of the Cell DOI: 10.1111/boc.201100011 ...
In general, fluorescent proteins generate far fewer photons than do synthetic dyes, such as the carbocyanines (Cy3 and Cy5) or ...
The bleachout dyes useful in the photosystems of this invention are polymethine dyes such as the cyanines, carbocyanines, ... and I, and bleachable polymethine dye selected from the group consisting of cyanines, carbocyanines, merocyanines, styryl dyes ... carbocyanines, merocyanines, styryl dyes and their higher vinylene homologs and wherein said dye is sensitive to light of wave- ... carbocyanines, merocyanines, styryl dyes and their higher vinylene homologs and wherein the solvent for said dye being a non- ...
Animals; Carbocyanines; Female; Fluorescent Dyes; Male (all 7) Animals; Carbocyanines; Female; Fluorescent Dyes; Male; ...
Multi-colour direct STORM with red emitting carbocyanines. Biology of the Cell. 104, (4), 229-237 (2012). ...
USPTO has issued the company patent number 7,465,810 for "Reactive 1,3-Crosslinked Carbocyanines and Their Bioconjugates." ... USPTO has issued the company patent number 7,465,810 for "Reactive 1,3-Crosslinked Carbocyanines and Their Bioconjugates." ... USPTO has issued the company patent number 7,465,810 for "Reactive 1,3-Crosslinked Carbocyanines and Their Bioconjugates." ...
Other dyes (rhodamines, carbocyanines, etc.) can be used, but laser 102, beamsplitters 108 and 106, and filters 138 and 124 may ...
... carbocyanines, azacarbocyanines and their isomers, diazacarbocyanines and their isomers, tetraazacarbocyanines or hemicyanines. ...
The photosensitizers belong to one of the following categories: ketones and their derivatives, carbocyanines and methines, ...
Aylmore, M. G., Graham, J. & Johnson, A., 1992, The 1992 Joint Conference on Electron Microscopy and Cell Biology. Griffin, B. J., Johnson, A. W. S., Kuo, J. & Lincoln, F. J. (eds.). Perth, Western Australia: Appletype Publications, Vol. 1. p. 44-44 Research output: Chapter in Book/Conference paper › Conference paper ...
... a family of nanovesicles proposed as scaffolds for the nanostructuration of commercial lipophilic carbocyanines ... ...
keywords = "Animals, Body Patterning, Carbocyanines, Chick Embryo, Ectoderm, Fibroblast Growth Factor 4, Fibroblast Growth ...
Here, we explored the mechanistic properties of a specific group of NIR heptamethine carbocyanines including MHI-148 dye we ...
... carbocyanines, hemicyanines, etc. Photographic systems in which the above sensitizing dyes can be used include a facsimile ...
... carbocyanines, Phenoxazone, Carbazine, oxazines, and rhodamines insbesonde re are suitable, but preferably those dyes (600-1000 ...
Multi-color direct STORM with red emitting carbocyanines. Lampe et al have developed a novel variant of direct STORM (dSTORM) ... Research Paper: Multi-color direct STORM with red emitting carbocyanines, Biology of the Cell (2012), DOI: 10.1111/boc. ...
  • Carbocyanines with short alkyl tails attached to the imine nitrogens are employed both as membrane-potential sensors ( Slow-Response Probes-Section 22.3 ) and as organelle stains for mitochondria and the endoplasmic reticulum ( Probes for Mitochondria-Section 12.2 , Probes for the Endoplasmic Reticulum and Golgi Apparatus-Section 12.4 ). (thermofisher.com)
  • Their extinction coefficients are somewhat larger and fluorescence quantum yields much larger than those of carbocyanines such as DiI. (thermofisher.com)
  • In particular, carbocyanines with indocarbocyanine, indodicarbocyanine and indotricarbocyanine skeletons have high extinction coefficients and good fluorescence quantum yields [Licha, K. (2002) Contrast Agents for Optical Imaging (Review). (allindianpatents.com)
  • Carbocyanines are among the most strongly light-absorbing dyes known and have proven to be useful tools in several different areas of research. (thermofisher.com)