Carbocyanines: Compounds that contain three methine groups. They are frequently used as cationic dyes used for differential staining of biological materials.Emulsions: Colloids formed by the combination of two immiscible liquids such as oil and water. Lipid-in-water emulsions are usually liquid, like milk or lotion. Water-in-lipid emulsions tend to be creams. The formation of emulsions may be aided by amphiphatic molecules that surround one component of the system to form MICELLES.Silver: Silver. An element with the atomic symbol Ag, atomic number 47, and atomic weight 107.87. It is a soft metal that is used medically in surgical instruments, dental prostheses, and alloys. Long-continued use of silver salts can lead to a form of poisoning known as ARGYRIA.Quaternary Ammonium Compounds: Derivatives of ammonium compounds, NH4+ Y-, in which all four of the hydrogens bonded to nitrogen have been replaced with hydrocarbyl groups. These are distinguished from IMINES which are RN=CR2.Alkynes: Hydrocarbons with at least one triple bond in the linear portion, of the general formula Cn-H2n-2.Halogens: A family of nonmetallic, generally electronegative, elements that form group 17 (formerly group VIIa) of the periodic table.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Fat Emulsions, Intravenous: Emulsions of fats or lipids used primarily in parenteral feeding.Phosphorus Compounds: Inorganic compounds that contain phosphorus as an integral part of the molecule.Inorganic Chemicals: A broad class of substances encompassing all those that do not include carbon and its derivatives as their principal elements. However, carbides, carbonates, cyanides, cyanates, and carbon disulfide are included in this class.Nanowires: Nanometer-scale wires made of materials that conduct electricity. They can be coated with molecules such as antibodies that will bind to proteins and other substances.Materials Testing: The testing of materials and devices, especially those used for PROSTHESES AND IMPLANTS; SUTURES; TISSUE ADHESIVES; etc., for hardness, strength, durability, safety, efficacy, and biocompatibility.Biocompatible Materials: Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.Surface Properties: Characteristics or attributes of the outer boundaries of objects, including molecules.Telomerase: An essential ribonucleoprotein reverse transcriptase that adds telomeric DNA to the ends of eukaryotic CHROMOSOMES.G-Quadruplexes: Higher-order DNA and RNA structures formed from guanine-rich sequences. They are formed around a core of at least 2 stacked tetrads of hydrogen-bonded GUANINE bases. They can be formed from one two or four separate strands of DNA (or RNA) and can display a wide variety of topologies, which are a consequence of various combinations of strand direction, length, and sequence. (From Nucleic Acids Res. 2006;34(19):5402-15)Telomere: A terminal section of a chromosome which has a specialized structure and which is involved in chromosomal replication and stability. Its length is believed to be a few hundred base pairs.RNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)ImidesEndocytosis: Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.Clathrin-Coated Vesicles: Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles is covered with a lattice-like network of the protein CLATHRIN. Shortly after formation, however, the clathrin coat is removed and the vesicles are referred to as ENDOSOMES.Microscopy, Fluorescence: Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.Adaptor Protein Complex 2: An adaptor protein complex primarily involved in the formation of clathrin-related endocytotic vesicles (ENDOSOMES) at the CELL MEMBRANE.Clathrin: The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. Each molecule of clathrin consists of three light chains (CLATHRIN LIGHT CHAINS) and three heavy chains (CLATHRIN HEAVY CHAINS) that form a structure called a triskelion. Clathrin also interacts with cytoskeletal proteins.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Coated Pits, Cell-Membrane: Specialized regions of the cell membrane composed of pits coated with a bristle covering made of the protein CLATHRIN. These pits are the entry route for macromolecules bound by cell surface receptors. The pits are then internalized into the cytoplasm to form the COATED VESICLES.Inventors: Persons or entities that introduce a novel composition, device, or process, as well as improvements thereof.New JerseyRosaniline Dyes: Compounds that contain the triphenylmethane aniline structure found in rosaniline. Many of them have a characteristic magenta color and are used as COLORING AGENTS.Lissamine Green Dyes: Green dyes containing ammonium and aryl sulfonate moieties that facilitate the visualization of tissues, if given intravenously. They have mostly been used in the study of kidney physiology.Citrullus: A plant genus of the family CUCURBITACEAE known for the edible fruit.Intellectual Property: Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)Microscopy, Confocal: A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.Olfactory Bulb: Ovoid body resting on the CRIBRIFORM PLATE of the ethmoid bone where the OLFACTORY NERVE terminates. The olfactory bulb contains several types of nerve cells including the mitral cells, on whose DENDRITES the olfactory nerve synapses, forming the olfactory glomeruli. The accessory olfactory bulb, which receives the projection from the VOMERONASAL ORGAN via the vomeronasal nerve, is also included here.Zebrafish: An exotic species of the family CYPRINIDAE, originally from Asia, that has been introduced in North America. They are used in embryological studies and to study the effects of certain chemicals on development.Olfactory Pathways: Set of nerve fibers conducting impulses from olfactory receptors to the cerebral cortex. It includes the OLFACTORY NERVE; OLFACTORY BULB; OLFACTORY TRACT; OLFACTORY TUBERCLE; ANTERIOR PERFORATED SUBSTANCE; and OLFACTORY CORTEX.Olfactory Receptor Neurons: Neurons in the OLFACTORY EPITHELIUM with proteins (RECEPTORS, ODORANT) that bind, and thus detect, odorants. These neurons send their DENDRITES to the surface of the epithelium with the odorant receptors residing in the apical non-motile cilia. Their unmyelinated AXONS synapse in the OLFACTORY BULB of the BRAIN.Odors: The volatile portions of substances perceptible by the sense of smell. (Grant & Hackh's Chemical Dictionary, 5th ed)Kidney Glomerulus: A cluster of convoluted capillaries beginning at each nephric tubule in the kidney and held together by connective tissue.Chick Embryo: The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.Primitive Streak: A linear band of rapidly proliferating cells that begins near the posterior end of an embryo and grows cranially. Primitive streak is formed during GASTRULATION by the convergent migration of primary ectodermal cells (EPIBLAST). The knot at the tip of the streak is called HENSEN NODE.Histology, Comparative: The study of the similarities and differences in the structures of homologous tissues across various species.Gastrula: The developmental stage that follows BLASTULA or BLASTOCYST. It is characterized by the morphogenetic cell movements including invagination, ingression, and involution. Gastrulation begins with the formation of the PRIMITIVE STREAK, and ends with the formation of three GERM LAYERS, the body plan of the mature organism.Blastoderm: A layer of cells lining the fluid-filled cavity (blastocele) of a BLASTULA, usually developed from a fertilized insect, reptilian, or avian egg.Mesoderm: The middle germ layer of an embryo derived from three paired mesenchymal aggregates along the neural tube.Carboxylic Acids: Organic compounds containing the carboxy group (-COOH). This group of compounds includes amino acids and fatty acids. Carboxylic acids can be saturated, unsaturated, or aromatic.Free Radicals: Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.Monocarboxylic Acid Transporters: A family of proteins involved in the transport of monocarboxylic acids such as LACTIC ACID and PYRUVIC ACID across cellular membranes.Acrylic ResinsHydroxyl Radical: The univalent radical OH. Hydroxyl radical is a potent oxidizing agent.Dicarboxylic AcidsCoumaric Acids: Hydroxycinnamic acid and its derivatives. Act as activators of the indoleacetic acid oxidizing system, thereby producing a decrease in the endogenous level of bound indoleacetic acid in plants.

17beta-estradiol reduces tumor necrosis factor-alpha-mediated LDL accumulation in the artery wall. (1/1146)

Estrogens have direct effects on the vascular wall that may prevent the development of atherosclerosis. In particular, estrogens, such as 17beta-estradiol (estradiol), are known to have potent antioxidant activity. Tumor necrosis factor-alpha (TNF) is found in human atheroma and produces oxygen-derived free radicals. These oxygen-derived free radicals may modify low density lipoproteins (LDL) and increase LDL binding in the artery wall. We asked: 1) does TNF increase LDL accumulation in the artery wall and 2) can the TNF-mediated increase in LDL accumulation be prevented by the antioxidant activity of estradiol? Carotid arteries from ovariectomized 3-month-old rats were removed and perfused with fluorescently labeled LDL and arterial LDL flux was measured using quantitative fluorescence microscopy. In six arteries, addition of TNF (10 ng/ml) to the perfusate resulted in a 2.3-fold increase in the rate of LDL accumulation (1.50 +/- 0.37 ng/min per cm2 vs. 3.38 +/- 0.48 ng/min per cm2; P < 0.01). Estradiol (65 pg/ml) and alpha-tocopherol (6 mg/L) both attenuated TNF-mediated LDL accumulation (P < 0.05), indicating that TNF may exert its effects on LDL accumulation through cellular production of oxygen-derived free radicals. These results support an antioxidant role for estradiol in the protection against LDL accumulation in the artery wall and subsequent progression of atherosclerosis.  (+info)

Development of cephalic neural crest cells in embryos of Lampetra japonica, with special reference to the evolution of the jaw. (2/1146)

Neural crest cells contribute extensively to vertebrate head morphogenesis and their origin is an important question to address in understanding the evolution of the craniate head. The distribution pattern of cephalic crest cells was examined in embryos of one of the living agnathan vertebrates, Lampetra japonica. The initial appearance of putative crest cells was observed on the dorsal aspect of the neural rod at stage 20.5 and ventral expansion of these cells was first seen at the level of rostral somites. As in gnathostomes, cephalic crest cells migrate beneath the surface ectoderm and form three major cell populations, each being separated at the levels of rhombomeres (r) 3 and r5. The neural crest seems initially to be produced at all neuraxial levels except for the rostral-most area, and cephalic crest cells are secondarily excluded from levels r3 and r5. Such a pattern of crest cell distribution prefigures the morphology of the cranial nerve anlage. The second or middle crest cell population passes medial to the otocyst, implying that the otocyst does not serve as a barrier to separate the crest cell populations. The three cephalic crest cell populations fill the pharyngeal arch ventrally, covering the pharyngeal mesoderm laterally with the rostral-most population covering the premandibular region and mandibular arch. The third cell population is equivalent to the circumpharyngeal crest cells in the chick, and its influx into the pharyngeal region precedes the formation of postotic pharyngeal arches. Focal injection of DiI revealed the existence of an anteroposterior organization in the neural crest at the neurular stage, destined for each pharyngeal region. The crest cells derived from the posterior midbrain that express the LjOtxA gene, the Otx2 cognate, were shown to migrate into the mandibular arch, a pattern which is identical to gnathostome embryos. It was concluded that the head region of the lamprey embryo shares a common set of morphological characters with gnathostome embryos and that the morphological deviation of the mandibular arch between the gnathostomes and the lamprey is not based on the early embryonic patterning.  (+info)

Characterization of nodular neuronal heterotopia in children. (3/1146)

Neuronal heterotopia are seen in various pathologies and are associated with intractable epilepsy. We examined brain tissue from four children with subcortical or periventricular nodular heterotopia of different aetiologies: one with severe epilepsy following focal brain trauma at 17 weeks gestation, one with hemimegalencephaly and intractable epilepsy, one with focal cortical dysplasia and intractable epilepsy, and one dysmorphic term infant with associated hydrocephalus and polymicrogyria. The connectivity of nodules was investigated using histological and carbocyanine dye (DiI) tracing techniques. DiI crystal placement adjacent to heterotopic nodules revealed numerous DiI-labelled fibres within a 2-3 mm radius of the crystals. Although we observed labelled fibres closely surrounding nodules, the majority did not penetrate them. Placement of DiI crystals within nodules also identified a limited number of projections out of the nodules and in one case there was evidence for connectivity between adjacent nodules. The cellular and neurochemical composition of nodules was also examined using immunohistochemistry for calretinin and neuropeptide Y (NPY), which are normally expressed in GABAergic cortical interneurons. Within heterotopic nodules from all cases, numerous calretinin-positive neurons were identified, along with a few cell bodies and many processes positive for NPY. Calretinin-positive neurons within nodules were less morphologically complex than those in the cortex, which may reflect incomplete differentiation into an inhibitory neuronal phenotype. There were also abnormal clusters of calretinin-positive cells in the overlying cortical plate, indicating that the migratory defect which produces heterotopic nodules also affects development of the cortex itself. Thus, heterotopic nodules consisting of multiple neuronal cell types are associated with malformation in the overlying cortical plate, and have limited connectivity with other brain regions. This abnormal development of connectivity may affect neuronal maturation and consequently the balance of excitation and inhibition in neuronal circuits, leading to their epileptogenic potential.  (+info)

Identification of megalin/gp330 as a receptor for lipoprotein(a) in vitro. (4/1146)

Lipoprotein(a) [Lp(a)] is an atherogenic lipoprotein of unknown physiological function. The mechanism of Lp(a) atherogenicity as well as its catabolic pathways are only incompletely understood at present. In this report, we show that the low density lipoprotein receptor (LDLR) gene family member megalin/glycoprotein (gp) 330 is capable of binding and mediating the cellular uptake and degradation of Lp(a) in vitro. A mouse embryonic yolk sac cell line with native expression of megalin/gp330 but genetically deficient in LDLR-related protein (LRP) and a control cell line carrying a double knockout for both LRP and megalin/gp330 were compared with regard to their ability to bind, internalize, and degrade dioctadecyltetramethylindocarbocyanine perchlorate (DiI)-fluorescence-labeled Lp(a) as well as equimolar amounts of 125I-labeled Lp(a) and LDL. Uptake and degradation of radiolabeled Lp(a) by the megalin/gp330-expressing cells were, on average, 2-fold higher than that of control cells. This difference could be completely abolished by addition of the receptor-associated protein, an inhibitor of ligand binding to megalin/gp330. Mutual suppression of the uptake of 125I-Lp(a) and of 125I-LDL by both unlabeled Lp(a) and LDL suggested that Lp(a) uptake is mediated at least partially by apolipoprotein B100. Binding and uptake of DiI-Lp(a) resulted in strong signals on megalin/gp330-expressing cells versus background only on control cells. In addition, we show that purified megalin/gp330, immobilized on a sensor chip, directly binds Lp(a) in a Ca2+-dependent manner with an affinity similar to that for LDL. We conclude that megalin/gp330 binds Lp(a) in vitro and is capable of mediating its cellular uptake and degradation.  (+info)

Endocytic sorting of lipid analogues differing solely in the chemistry of their hydrophobic tails. (5/1146)

To understand the mechanisms for endocytic sorting of lipids, we investigated the trafficking of three lipid-mimetic dialkylindocarbocyanine (DiI) derivatives, DiIC16(3) (1,1'-dihexadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), DiIC12(3) (1,1'- didodecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), and FAST DiI (1,1'-dilinoleyl-3,3,3', 3'-tetramethylindocarbocyanine perchlorate), in CHO cells by quantitative fluorescence microscopy. All three DiIs have the same head group, but differ in their alkyl tail length or unsaturation; these differences are expected to affect their distribution in membrane domains of varying fluidity or curvature. All three DiIs initially enter sorting endosomes containing endocytosed transferrin. DiIC16(3), with two long 16-carbon saturated tails is then delivered to late endosomes, whereas FAST DiI, with two cis double bonds in each tail, and DiIC12(3), with saturated but shorter (12-carbon) tails, are mainly found in the endocytic recycling compartment. We also find that DiOC16(3) (3,3'- dihexadecyloxacarbocyanine perchlorate) and FAST DiO (3, 3'-dilinoleyloxacarbocyanine perchlorate) behave similarly to their DiI counterparts. Furthermore, whereas a phosphatidylcholine analogue with a BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) fluorophore attached at the end of a 5-carbon acyl chain is delivered efficiently to the endocytic recycling compartment, a significant fraction of another derivative with BODIPY attached to a 12-carbon acyl chain entered late endosomes. Our results thus suggest that endocytic organelles can sort membrane components efficiently based on their preference for association with domains of varying characteristics.  (+info)

Reactive oxygen metabolites increase mitochondrial calcium in endothelial cells: implication of the Ca2+/Na+ exchanger. (6/1146)

In endothelial cells, a bolus of hydrogen peroxide (H2O2) or oxygen metabolites generated by hypoxanthine-xanthine oxidase (HX-XO) increased the mitochondrial calcium concentration [Ca2+]m. Both agents caused a biphasic increase in [Ca2+]m which was preceded by a rise in cytosolic free calcium concentration [Ca2+]c (18 and 6 seconds for H2O2 and HX-XO, respectively). The peak and plateau elevations of [Ca2+] were consistently higher in the mitochondrial matrix than in the cytosol. In Ca2+-free/EGTA medium, the plateau phase of elevated [Ca2+] evoked by H2O2 due to capacitative Ca2+ influx was abolished in the cytosol, but was maintained in the mitochondria. In contrast to H2O2 and HX-XO, ATP which binds the P2Y purinoceptors induced an increase in [Ca2+]m that was similar to that of [Ca2+]c. When cells were first stimulated with inositol 1,4, 5-trisphosphate-generating agonists or the Ca2+-ATPase inhibitor cyclopiazonic acid (CPA), subsequent addition of H2O2 did not affect [Ca2+]c, but still caused an elevation of [Ca2+]m. Moreover, the specific inhibitor of the mitochondrial Ca2+/Na+ exchanger, 7-chloro-3,5-dihydro-5-phenyl-1H-4.1-benzothiazepine-2-on (CGP37157), did not potentiate the effects of H2O2 and HX-XO on [Ca2+]m, while causing a marked increase in the peak [Ca2+]m and a significant attenuation of the rate of [Ca2+]m efflux upon addition of histamine or CPA. In permeabilized cells, H2O2 mimicked the effects of CGP37157 causing an increase in the basal level of matrix free Ca2+ and decreased efflux. Dissipation of the electrochemical proton gradient by carbonylcyanide p-(trifluoromethoxy) phenylhydrazone (FCCP), and blocade of the Ca2+ uptake by ruthenium red prevented [Ca2+]m increases evoked by H2O2. These results demonstrate that the H2O2-induced elevation in [Ca2+]m results from a transfer of Ca2+ secondary to increased [Ca2+]c, and an inhibition of the Ca2+/Na+ electroneutral exchanger of the mitochondria.  (+info)

Optical mapping of neural network activity in chick spinal cord at an intermediate stage of embryonic development. (7/1146)

We have applied multiple-site optical recording of transmembrane potential changes to recording of neuronal pathway/network activity from embryonic chick spinal cord slice preparations. Spinal cord preparations were dissected from 8-day-old chick embryos at Hamburger-Hamilton stage 33, and transverse slice preparations were prepared with the 13th cervical spinal nerve or with the 2nd or 5th lumbosacral spinal nerve intact. The slice preparations were stained with a voltage-sensitive merocyanine-rhodanine dye (NK2761). Transmembrane voltage-related optical (dye-absorbance) changes evoked by spinal nerve stimulation with positive square-current pulses using a suction electrode were recorded simultaneously from many loci in the preparation, using a 128- or 1,020-element photodiode array. Optical responses were detected from dorsal and ventral regions corresponding to the posterior (dorsal) and anterior (ventral) gray horns. The optical signals were composed of two components, fast spike-like and slow signals. In the dorsal region, the fast spike-like signal was identified as the presynaptic action potential in the sensory nerve and the slow signal as the postsynaptic potential. In the ventral region, the fast spike-like signal reflects the antidromic action potential in motoneurons, and the slow signal is related to the postsynaptic potential evoked in the motoneuron. In preparations in which the ventral root was cut microsurgically, the antidromic action potential-related optical signals were eliminated. The areas of the maximal amplitude of the evoked signals in the dorsal and ventral regions were located near the dorsal root entry zone and the ventral root outlet zone, respectively. Quasiconcentric contour-line maps were obtained in the dorsal and ventral regions, suggesting the functional arrangement of the dorsal and ventral synaptic connections. Synaptic fatigue induced by repetitive stimuli in the ventral synapses was more rapid than in the dorsal synapses. The distribution patterns of the signals were essentially similar among C13, LS2, and LS5 preparations, suggesting that there is no difference in the spatiotemporal pattern of the neural responses along the rostrocaudal axis of the spinal cord at this developmental stage. In the ventral root-cut preparations, comparing the delay times between the ventral slow optical signals, we have been able to demonstrate that neural network-related synaptic connections are generated functionally in the embryonic spinal cord at Hamburger-Hamilton stage 33.  (+info)

Correct targeting of dihydropyridine receptors and triadin in dyspedic mouse skeletal muscle in vivo. (8/1146)

Excitation-contraction coupling in skeletal muscle involves junctions (triads and dyads) between sarcoplasmic reticulum (SR) and transverse (T) -tubules. Two proteins of the junctional SR, ryanodine receptors (RyRs) and triadin and one protein of T tubules, dihydropyridine receptors (DHPRs) are located at these junctions. We studied the targeting of DHPRs and triadin to T-tubules and SR in skeletal muscles of dyspedic mouse embryos lacking RyR1. In normal differentiating muscle fibers DHPRs, triadin and RyRs are located in intensely immunolabeled foci that are randomly distributed across the fiber. Correlation with electron microscopy and with previous studies indicates that the foci represent the location of triads and dyads. In dyspedic fibers DHPRs and triadin antibodies stain internal foci of the two proteins; RyR antibodies are completely negative. The appearance and location of the foci in dyspedic fibers is similar to that of normal muscle, but their fluorescent intensity is weaker. The SR Ca-ATPase has more diffuse distribution than triadin in both normal and dyspedic fibers. These observations indicate that an interaction with RyRs is not necessary for the appropriate targeting of DHPRs or triadin to junctional domains of T tubules and SR respectively.  (+info)

We prepared silica NPs doped with two far-red absorption cyanine dyes, FR670 and Cy5, using the microemulsion method. At these wavelengths there is less autofluorescence from biological molecules, solvents and substrates. The effects of changing surfactant, pH and dye loading on NP morphology and colloidal stability were investigated using transmission electron microscopy and photo correlation spectroscopy. We found that the incorporation of Cy5 and FR670 was only possible using negatively charged surfactants. We propose a method for the facile incorporation of cyanine dye based on the electrostatic repulsion between negatively charged dye molecules and the negatively charged surfactant, present at the water oil interface, which drives the dye into the silica NP forming inside the water phase. The absorption, fluorescence and quantum efficiency of the NPs were also measured and compared against free dye labels. The optimal fluorescences for FR670 and Cy5 dye doped NPs, were 178 and 83 times brighter
Cyanine is the non-systematic name of a synthetic dye family belonging to polymethine group. The word cyanin is from the English word "cyan", which conventionally means a shade of blue-green (close to "aqua") and is derived from the Greek κυάνεος/κυανοῦς kyaneos/kyanous which means a somewhat different color: "dark blue". Cyanines were and are still used in industry, and more recently in biotechnology (labeling, analysis). Cyanines have many uses as fluorescent dyes, particularly in biomedical imaging. Depending on the structure, they cover the spectrum from IR to UV. There are a large number reported in the literature. There are three types of cyanines: Streptocyanines or open chain cyanines: R2N+=CH[CH=CH]n-NR2 (I) Hemicyanines: Aryl=N+=CH[CH=CH]n-NR2 (II) Closed chain cyanines: Aryl=N+=CH[CH=CH]n-N=Aryl (III) where two nitrogens are joined by a polymethine chain. Both nitrogens are each independently part of a heteroaromatic moiety, such as pyrrole, imidazole, thiazole, ...
We have used carbocyanine dyes (DiI and DiO) to generate fate maps for the epiblast layer of the chick embryo between stage X and the early primitive streak stage (stages 2-3). The overall distribution of presumptive cell types in these maps is similar to that described for other laboratory species …
DNA microarray technology is a high throughput technique by which the expression of the whole genome is studied in a single experiment. In dual label experiments the fluorescent dyes Cy 3 and Cy 5 are used to label the two RNA samples co-hybridized on a same array. Recently two more dyes have been proposed (Alexa 488 and Alexa 594) allowing the simultaneous hybridization of three or four samples. Forster et al. [2] have evaluated triple-target microarray by comparing results of single-target, dual-target and triple-target microarrays. They have concluded that the use of triple-target microarray is valid from an experimental point of view. One year later, Staal et al. [7] have investigated the four-target microarray experiments. Their approach differs from that of [2], but their conclusions are in fair agreement. Their study has shown that Alexa 594 is best suited as a third dye and that Alexa 488 can be applied as a fourth dye on some microarray types. These extensions of the microarray ...
Woolery G.L.; Powers L.; Winkler M.; Solomon E.I.; Spiro T.G., 1984: Extended x ray absorption fine structure studies of bi nuclear copper site of oxy hemo cyanin deoxy hemo cyanin metaquo hemo cyanin met fluoro hemo cyanin and met azido hemo cyanin from arthropods and mollusks
This section illustrates a culture of HeLa cells that were labeled with the lipophilic cell tracer carbocyanine dye, Dil, which targets membranes. The absorption maximum of Dil is 549 nanometers and the emission maximum occurs at 565 nanometers (in the yellow region of the visible light spectrum). Fluorescence intensity arising from labeled cytoskeletal membrane components is quite evident in the image even through the probe emission peak is 30 nanometers below the Y-2E/C filter set dichromatic mirror cut-on wavelength.
S. B. Anantharaman et al., Strongly Red-Shifted Photoluminescence Band Induced by Molecular Twisting in Cyanine (Cy3) Dye Films, The Journal of Physical Chemistry C, 2017, DOI: 10.1021/acs.jpcc.7b01412 (open access). http://pubs.acs.org/doi/suppl/10.1021/acs.jpcc.7b01412. Besides in silver halide photography, industrial applications of dye aggregates are hampered due to difficulties in producing aggregates reproducibly, with controlled size and perfection and avoiding complex manufacturing processes such as Langmuir-Blodgett techniques.. While cyanine dye aggregates are best known for intense and narrow absorption bands, our films stick out for prominent scattering in the absorption band (resonance light scattering). Similar to localized surface plasmon resonances (LSPR), scattering can be explained with the coherently oscillating electron cloud of the aggregate in response to incident light. Because dye aggregate extinction has narrowest bandwidths, the wavelength selectivity exceeds the ...
Both methylmercury (MeHg) and inorganic divalent mercury (Hg++) alter the flux of ions and small molecules across nerve terminal membranes by mechanisms that may involve membrane depolarization. We compared the effects of MeHg and Hg++ on plasma (psi p) and mitochondrial membrane potentials (psi m) in synaptosomes using the potentiometric carbocyanine dye 3,3-diethylthiadicarbocyanine iodide [diS-C2(5)]. Both mercurials (1-20 microM) produced concentration-dependent increases in dye fluorescence after 5 min of exposure which were not altered by removal of Ca++ from the medium. To determine directly effects of mercurials on psi p, predepolarization of psi m using NaN3 and oligomycin was necessary. Under this condition, MeHg- and Hg(++)-induced increases in fluorescence were associated with depolarization of psi p. A second approach was used to assess changes in psi p. In synaptosomes, the magnitude of the increase in fluorescence resulting from depolarization of psi p with a stimulus of constant ...
The phosphorylation state of human and bovine spinal cord neurofilaments (NF) was studied by direct phosphate analysis and carbocyanine dye ("Stains-all") binding to NF polypeptides resolved on SDS-polyacrylamide gels. Electrophoretically purified NF-H (200 kDa), NF-M (160 kDa), and NF-L (68 kDa) of human origin contained 24, 18, and 4 mol phosphate/mol protein, whereas bovine NF contained 53, 23, and 5 mol phosphate/mol protein, respectively. Incubation of NF preparations with E. coli alkaline phosphatase removed about 55% of the phosphate from NF-H, about 30% of the phosphate from both human and bovine NF-M, but did not change the phosphate content of NF-L. This treatment also inhibited or substantially reduced the binding of electroblotted NF-H and NF-M to 2 anti-NF monoclonal antibodies known to recognize phosphorylated sites on projection side arms. "Stains-all" was found to be a very sensitive probe for detection of phosphorylated cytoskeletal proteins. Without the phosphatase treatment, ...
Cyanine fluorophores are encoded as non-canonical amino acids to produce functional proteins in cell-free translation systems and live cells for single-molecule imaging.
Methods of tracking cells in vivo and for determining in vivo cell lifetimes. Cells are labelled with cyanine dyes and detection is by measuring fluorescence, absorbance, or by detecting nuclear magnetic resonance probes included in the cyanine dyes. Using the invented methods, for example, red blood cell and platelet lifetimes are determined. Also, cells are tracked to determine sites of primary or metastatic tumors, or sites of occult infection. Further, rates at which cells pass through vessels is used to determine blood vessel patency and platelet aggregation.
Antibodies. Monoclonal antibodies specific for synaptophysin, microtubule-associated protein 2 (MAP-2), actin, and GABAA were purchased from Boehringer Mannheim (Indianapolis, IN); those for agrin (m247 and m33) were from StressGen Biotechnologies (Victoria, British Columbia, Canada). Antibodies specific for neuron-specific enolase, synapsin-I, glutamic acid decarboxylase, postsynaptic density 95 (PSD-95), and CREB were purchased from Polysciences (Warrington, PA), Molecular Probes (Eugene, OR), Chemicon (Temecula, CA), Affinity Bioreagents (Golden, CO), and New England Biolabs (Beverly, MA), respectively. Antibodies against NR1 were a kind gift from Dr. R. Huganir (Johns Hopkins University, Baltimore, MD). A polyclonal serum specific for GluR1 was raised as described (Molnar et al., 1994). Secondary antibodies conjugated with indocarbocyanine, FITC, and Texas Red were purchased from Jackson ImmunoResearch (West Grove, PA).. Oligonucleotides. Oligonucleotides were synthesized by Oligos Etc. ...
TSA® Plus Cyanine 5 (Cy5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy5 in the localized area of the probe or antibody. ...
TSA® Plus Cyanine 3.5 (Cy3.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3.5 in the localized area of the probe or antibody.
Molecular beacons were postulated to be a good fit for our design, as their complementary design to segments of the amplified DNA would allow for easy fluorescent quantification. Molecular beacons in our assays were purchased from IDT ($660 for 1580 samples ). They consist of a stem-loop structure with one end attached to a fluorophore (5′ 6 FAM) and the opposite end attached to a quencher molecule (3′ IOWA BLACK FQ). When the stem-loop structure is closed, the fluorophore is in contact with and quenched by the quencher molecule, and fluorescence is inhibited. However, when the stem-loop opens up upon binding to the amplified DNA oligomers, the quencher and fluorophore are no longer in contact and thus allows for the detection of fluorescence. On the other hand, SYBR Gold (ThermoScientific; $120 for 50,000 samples) is a fluorescent nucleic acid intercalator (cyanine dye) that stains and allows fluorescence detection of ssDNA, dsDNA, and RNA with signal enhancement of greater than 1000 fold. ...
XenoLight DiR lipophilic, NIR fluorescent cyanine dye ideal for staining cytoplasmic membrane. Ideal for in vivo imaging of T-cell or stem cell homing.
XenoLight DiR lipophilic, NIR fluorescent cyanine dye ideal for staining cytoplasmic membrane. Ideal for in vivo imaging of T-cell or stem cell homing.
Dilämmastikoksiid Elma Messer is a medicine available in a number of countries worldwide. A list of US medications equivalent to Dilämmastikoksiid Elma Messer is available on the Drugs.com website.
Tsídii Deílʼésígíí éí "Ciconiiformes" daolyé, ndaʼałkaahíkʼehjí; díí saadígíí éí "Tsídii bidaanézí Ndahalinígíí" óolyé.. Táłtłʼááh álééh dóó toohjįʼ ndiigaii dóó yáhashjool éí "Ciconiiformes"-jįʼ atah daasdzoh. Łaʼ ndaʼałkaahí ádaaníigo tsídiidaatsohí éí ałdóʼ díí tsídii dahyikahjįʼ atah yisdzoh daaní; náánáłaʼ ndaʼałkaahí éí doo bił ákódaatʼée da.. Díí tsídii éí tʼáá ałtso bijáád nineez, áádóó éí tábąąhgi naakai łeh; łaʼ éí halgaiigi dóó tłʼoh dahólǫ́ǫgi naakai ałdóʼ. Nááná éí bidaaʼ nineez łeh. Łaʼ éí łóóʼ dóó naʼashǫ́ʼii haalzhéehgo deílʼéés, náánáłaʼ éí tʼóó chʼosh deildeeł.. ...
http://www.fuzionproductions.com/after-madhubala-it-is-do-dil-ek-jaan-for-nautanki-films-the-same-creative-team-again-stirring-up-another-love-story-in-the-beautiful-valley-of-kashmir/ ...Rima...2013-06-05 16:46:48 | 3616695 | Do Dil Ek Jaan Forum
Kuch Ehsason Ke Saye Dil Ko Chu Jate Hain, Kuch Manzar Dil Mein Utar Jate Hain, Bejan Gulshan Mein Bhi Phool Khil Jate Hain, Jab Zindagi Mein Aap Jaise Dost Mil Jate Hain ...
Uptake of CSC-EVs by MSCsCSC-EVs stained with Vybrant Dil (in red) were incubated for 6, 24, 48 and 72 hours with MSCs. Cytoplasm staining of MSCs was obtained
抗鼠Cy5®(ab6563)经ELISA, IHC-P, IHC-Fr, Flow Cyt, ICC/IF实验严格验证。产品经预吸附处理(去除交叉反应)。被16篇发表文献引用。其他Cy5®偶联二抗可供选择。
Gentaur molecular products has all kinds of products like :search , Gene Link \ Cy5.5 50 nmol scale \ 26-6460-05 for more molecular products just contact us
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7. "厚膜集成电路汽车用喊话机"、"1205公安部用厚膜集成电路对讲机"、"20W大功率厚膜集成电路电影放映机还音设备"、"厚膜电阻网络"、"CY2A环氧包封电容器"、"CBB-12系列组合膜电容器"、"CBB-15系列组合膜电容器"等6项新产品分别获南京市科委、南京电子元件公司、南京无线电公司优秀新产品奖和新产品设计奖 (1977-1984年)(负责组织、实施及参与) ...
Fomina M. V., Nikiforov A. S., Gromov S. P. Modern approaches to the synthesis and prospects for the use of cyanine dyes containing functional groups in the n-substituents // Russian Chemical Reviews. - 2016. - Vol. 85, no. 7. - P. 684-699. Methods for the synthesis of mono-, tri-, penta- and heptamethine cyanine dyes containing functional groups in N-substituents are considered. Approaches suitable for the preparation of both symmetrical and unsymmetrical cyanine dyes are presented. Examples of the practical use of this type of cyanine dyes as fluorescent labels and probes in biology and medicine, as well as of components of photoactive supramolecular structures, photosensitizers and photo- and electroluminescent materials are given. The bibliography includes 106 references. [ DOI ...
BACKGROUND: Burkitt lymphoma is a fast-growing mature B cell malignancy, whose genetic hallmark is translocation and activation of the c-myc gene. Prompt multiagent immunochemotherapy regimens can have favorable outcomes, but prognosis is poor in refractory or relapsed disease. We previously identified a novel family of near-infrared heptamethine carbocyanine fluorescent dyes (HMCD or DZ) with tumor-homing properties via organic anion-transporting peptides. These membrane carriers have uptake in tumor cells but not normal cells in cell culture, mouse and dog tumor models, patient-derived xenografts, and perfused kidney cancers in human patients. METHODS: Here we report the cytotoxic effects of a synthesized conjugate of DZ with cisplatin (CIS) on B cell lymphoma CA46, Daudi, Namalwa, Raji, and Ramos cell lines in cell culture and in xenograft tumor formation. Impaired mitochondrial membrane permeability was examined as the mechanism of DZ-CIS-induced lymphoma cell death. RESULTS: The new ...
Accumulating evidence is revealing the essential role of immune system in cancer treatment. Certain chemotherapeutic drugs can potently induce the release of cell death associated molecular patterns (CDAMPs), which accompanies cancer cell demise. CDAMPs can engage corresponding receptors on immune cells and stimulate immune responses to achieve long-term tumor control (Ma et al., 2013; Ma et al., 2014; Yang et al., 2015). Among reported CDAMPs, calreticulin (CALR), ATP and HMGB1 are well known for their immune-stimulatory effect. Here we describe the assays that we applied to measure cell death and these CDAMPs. Briefly, cell death can be analyzed by co-staining of 4,6-diamidino-2-phenylindole (DAPI) with 3,3-Dihexyloxacarbocyanine Iodide [DiOC6(3)] or Annexin V. CALR exposure on the cell membrane can be detected by flow cytometry. ATP and HMGB1 release can be quantified by luminescence assay and ELISA assay respectively.
The green fluorescent, lipophilic carbocyanine DiOC18(3) is widely used as a lipophilic tracer.,,The green fluorescent, lipophilic carbocyanine DiOC18(3) is widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.
Cy3 and Cy5 are registered trademarks of Amersham Pharmacia Biotech Inc, Alexa Fluor is a registered trademark of Molecular Probes Inc ...
Page contains details about Fab-conjugated GGLG/chol/PEG-DSPE/Mal-PEG-Glu2C18/DiIC18(7) liposomes . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
This invention relates to a dye donor element for laser-induced thermal dye transfer comprising a support having thereon a dye layer comprising an image dye in a polymeric binder and a cyanine infrared absorbing dye associated therewith, and wherein said layer also has a nitrosonaphthol ferrous complex associated therewith.
APC/Cyanine7 anti-human CD62L Antibody - CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1.
Cytoskeleton Inc provides live cell Spirochrome fluorescent Flipper-TR, tension, STED, SIM, widefield, tirf, flim, confocal, using CellMask, Cellvue, Cellbrite, Membrite, Cy5, YFP, GFP, lipophilic cyanine dyes to cell motility, phagocytosis endocytosis.
Congratulations to Dr. D. Lansing Taylor for his appointment to Distinguished Professor.. Dr. Taylor has been with the department since his return to academia in 2010. Prior to that, he held academic appointments at Harvard University and Carnegie Mellon University.. During his time as CMU, he and Dr. Alan Waggoner co-found Biological Detection Systems (BDS) to commercialize the multi-color cyanine dyes and research imaging platform. The company was later acquired by Amersham (now GE Life Sciences). He left CMU in 1997 to found Cellomics, Inc., which developed High Content Screening and later became a part of ThermoFisher Scientific. He then moved on to found Cellumen, which developed a predictive safety assessment platform using primary hepatocytes, multiplexed panels of reagents, reference safety databases and computational biology. He was CEO of Cellumen from 2004 until 2010 when it became part of Cyprotex, a British CRO. He also co-found Cernostics, Inc., a fluorescence-based, tissue systems ...
Near infrared fluorophore useful for non-invasive in vivo imaging. Absorption properties are similar to indocyanine green (ICG), but possesses higher fluorescence quantum yield.
Bernd Fritzsch is the author of this article in the Journal of Visualized Experiments: Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology
Tsídii éí tʼáá ałtso bijáád naakigo hólǫ́, áádóó éí bitʼaʼ dahólǫ́; łaʼ tsídii éí doo ndaatʼáa da ndi, tʼóó niʼgóó naakai łeh (tsídiitsoh da).. Tsídiitsoh éí yéego ntsaa (náhástʼéigo da adéesʼeez[?] éí áníłtso), dahiitį́hii łaʼ éí tʼáá díkwíí inch tʼéiyá ádaníłnééz. Tsídii éí tʼáá ałtso bidaaʼ ntłʼiz (dóó nineez łeh); áádóó éí biwooʼ ádaadin. Nááná éí bitsʼin biiʼ haltsʼaaʼ łeh.. ...
電源輸入、限流電阻、外接驅動穩壓、喇叭輸出端的端子台都排排站好在圖中的下方 ,,. JC-3正負電源輸入的點在PCB下方,正電源接到CN5、負電源接到CN6、濾波電容中點(GND)接到CN3、CN4其中一個。接好正負電源之後記得將JP1和JP2分別短路,因為JP1和JP2是預留給驅動穩壓使用的,一般情況直接短路就可以了。. 上電調整測試說明 -上電調整輸入級偏流. 接好正負電源之後,在上電之前,保險起見,分別將JP3、JP4的兩點之間串上套件內附10 Ohm /0.5W的限流電阻充當保險絲用,。. 接下來將SVR3逆時針轉到底(轉到底時會發出喀喀的聲音)將偏流調到最小,然後上電,如果發現兩顆10 ...
A Long-Wavelength Fluorescent Squarylium Cyanine Dye Possessing Boronic Acid for Sensing Monosaccharides and Glycoproteins with High Enhancement in Aqueous Solution. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Membrane environment and fluidity can modulate the dynamics and interactions of membrane proteins and can thereby strongly influence the function of cells and organisms in general. In this work, we demonstrate that trans-cis isomerization of lipophilic dyes is a useful parameter to monitor packaging and fluidity of biomembranes. Fluorescence fluctuations, generated by trans-cis isomerization of the thiocarbocyanine dye Merocyanine 540 (MC540), were first analyzed by fluorescence correlation spectroscopy (FCS) in different alcohol solutions. Similar isomerization kinetics of MC540 in lipid vesicles could then also be monitored, and the influence of lipid polarity, membrane curvature, and cholesterol content was investigated. While no influence of membrane curvature and lipid polarity could be observed, a clear decrease in the isomerization rates could be observed with increasing cholesterol contents in the vesicle membranes. Finally, procedures to spatially map photoinduced and thermal ...
Cyanine dye bioconjugates useful for diagnostic imaging and therapy are disclosed. The conjugates include several cyanine dyes with a variety of bis- and tetrakis (carboxylic acid) homologs. The compo
Late actor Sushant Singh Rajputs last movie, Dil Bechara will be released directly on an OTT (over-the-top) platform.Disney+ Hotstar tweeted on Thursday that Dil Bechara will be streamed on Disney+ H
A copolymer comprising 70 to 100 mole percent of repeating units comprising a formazan dye radical and a cyanine dye radical. The copolymers are useful in recording layers of optical recording elements.
[QUOTE=surbhimathur] [QUOTE=cuteswayu]anybdy?[/QUOTE]Dosttt[/QUOTE] sur.... ... | Page: 114 | 3184888 | Channel V Dil Dosti Dance Forum
Page 3 of 4 - mrofinu100186 and DIL.tmp will not LEAVE! - posted in Virus, Spyware, Malware Removal: ComboFix 08-04-04.1 - Owner 2008-04-06 17:03:00.2 - NTFSx86Microsoft Windows XP Professional 5.1.2600.2.1252.1.1033.18.383 [GMT -4:00]Running from: C:\Documents and Settings\Owner\Desktop\ComboFix.exe.TimedOut: progfile.dat ((((((((((((((((((((((((((((((((((((((( Other Deletions ))))))))))))))))))))))))))))))))))))))))))))))))).C:\Program Files\Tempora...
Watch Dil Jo Bhi Kahey... official movie trailer in HD. During the British rule in India, several Indians were lured by the British to travel to distant Mauritius where they would have a better life.
Source: BD-50 @ DDR Size: 1.77 GB General Format : M2TS Overall bit rate : 5 513 Mbps Video Format : AVC Format/Info : Advanced Video Codec Format pro
The structure was refined using the REFMAC program. The R value is 0.19394 for 43656 reflections in the resolution range 20.0 to 1.9 Ångstroms with Fobs , 0.0 sigma(Fobs) and with I , 0.0 sigma(I) ...
Tel: +44 90) 161 275 5500 Location: Centre for Occupational & Environmental Health. 4th floor, block C, Ellen Wilkinson Bldg,. Oxford Road,. Manchester M13 9PL. Websites ...
1) Pokud se zeptala studenta, kter zd dil byt po d dovi a hypot ku si ve skute nosti v bec vz t v nejbli ch deseti letech nepl nuje, tak e si o tom nic nezji oval, tak nevid m d vod, pro by m l n jak rozum t hypot k m.. 2) Fakticky ani ne ekl nic patn . Slovem rok se v e tin rozum to, co plat m v iteli nav c. Pokud by byl rok dv procenta, tak je to t ch edes t tis c a v po et na eho mlad ho mu e sed . Probl mem je, e banky pravd podobn nab z hypot ku nikoliv s rokem dv procenta, ale s (ro n ?) rokovou m rou/sazbou dv procenta. Jsem teoretik (taky jsem d dil), ale zd se mi, e m slo na hlav m sp pan novin ka, kdy mlad mu mu i zapomn la vlo it do st term n rokov sazba, kter m by jeho v po et ztrapnila tak, jak m la v myslu ...
Dil Station (U) charge* apply Theee charges do not apply to pereon-*>person. com, rwtal gu^. ca*r cd, co^ caH. crt cr^g^ 10 nom^ rnimb^. o< 10 lime ...
The DIL was founded in 1983 and is situated in the county of Osnabrück, one of the regions with the highest numbers of full-time employees and hi .. .
V Turecku po zma en m pokusu o vojensk pu za aly masivn istky v policii a arm d . Odej t muselo odej t na 8 000 policist , des tky gener l jsou pod policejn m dohledem. Prezident Recep Tayyip Erdogan na dil arm d nasadit bojov letadla F-16 k hl dk m nad zem .
Filiz Aky ld z Ak ay, Serdar Bayata, Tuna Semerci, Murat Ye il, O uzhan Toklu, Erdin Ar kan, Selcen Yakar T l ce, Mehmet K seo lu, dil Ko a ra Ya ...
The lipophilic cation JC‐1 (5,5′,6,6′‐tetrachloro‐1,1′,3,3′‐tetraethyl‐benzimidazolyl carbocyanine iodide) has been used for more than 20 years as a specific dye for measuring mitochondrial membrane potential (ΔΨm)
ORCID: 0000-0001-8401-5223, Moffatt, Colin and Smith, Robert B (2013) N-Alkylated 2,3,3-trimethylindolenines and 2-methylbenzothiazoles. Potential lead compounds in the fight against Saccharomyces cerevisiae infections. European journal of medicinal chemistry, 64 . pp. 222-227. ISSN 1768-3254 Okoh, Adeyi Okoh, Bisby, RH, Lawrence, Clare Louise, Rolph, Carole Elizabeth and Smith, Robert B (2013) Promising near-infrared non-targeted probes: benzothiazole heptamethine cyanine dyes. Journal of Sulfur Chemistry, 35 (1). pp. 42-56. ISSN 1741-5993 Brocklesby, Kayleigh L., Johns, Stephen C., Jones, Allison E., Sharp, Duncan and Smith, Robert B (2013) Smart bandages - A colourful approach to early stage infection detection & control in wound care. Medical Hypotheses, 80 (3). pp. 237-240. ISSN 03069877 Sharp, Duncan, Gladstone, Patience, Smith, Robert B, Forsythe, Stephen and Davis, James (2010) Approaching intelligent infection diagnostics: Carbon fibre sensor for electrochemical pyocyanin detection. ...
S p 1963 R. H. SPRAGUE ETAL 3,104,973 PHOTOGRAPHIC BLEACHING OUT OF CYAINE DYES Filed Aug. 5, 1960 BLUE @LLGHT GRLENQLLGHT RED 1.10m E I. l ,I 2 RED SENSITIVE; (CYAN DYE) 6652B}! SENSITIVE AGENTA DYE) BLUE SENS YnvE (YELLOW DYE SUBSTRATE (FILM OR PAPER) FIG. 1.. WHITE LIGHT CYAN DYE, c MAGENTA DYE, B YELLOW DYE, A SUBSTRATE TRANSMLTS TRANSMITS TRANSMITS BLUE I GREEN RED INVENTORS ROBERT E. SPRAGUE, HARRY L. FICHTIZR,Jr. w l LLlAM P HAMILTON ATTORNEY United States Patent 3,104,973 PHOTOGRAPHIC BLEACHJNG OUT OF CYAYINE DYES Robert H. Sprague, Cleveland, Harry L. Fichter, Jr., Lakewood, and William P. Hamilton, Cleveland, Chic, assignors to Horizons Incorporated, Cleveland, (lhio, a corporation of New Jersey Filed Aug. 5, 1960, Ser. No. 47,849 19 Claims. (Cl. 96-48) This invention relates to a direct positive photographic process. More particularly, the invention relates to a non-silver direct positive dye-bleach photographic process capable of producing full color photographic prints wherein the ...
Purified PHA-E is conjugated to cyanine 5 (Cy5) fluorescent dye. Cyanine dyes are better able to withstand the harsh dehydration and embedding conditions required for mounting sections in non-polar plastic media, and are brighter in a non-polar environment than in an aqueous medium. Advantages of plastic over aqueous mounting media are brightness, contrast, and longevity of the fluorescence. Cy5 has an approximate peak excitation wavelength of 650 nm and an emission wavelength at 670 nm. This product comes in a stabilized liquid form.. +2° to +8° C.. ...
Susan, I use DPX Mountant (Fluka Biochemika 44581) to mount Cy2, Cy3, Cy5 triple label slides. Heather Ho Clinical Lab Coordinator Medical College of Wisconsin On Wed, 25 Sep 2002, Susan Travers wrote: > does anybody know of a mounting media compatible with double labeling > using the fluorescent cyanine dyes (CY2 & CY3) and BCIP/NBT. These > particular fluorescent dyes wont withstand the anti-fading agent > phenylendiamine; the BCIP/NBT is compromised by xylene-miscible > mountants like permount and cytoseal. > > Any ideas? > > Thanks, > Susan Travers > Susan Travers, Ph.D. > Associate Professor > Ohio State University > College of Dentistry > Section of Oral Biology > 305 West 12th Avenue #192 > P.O. Box 182357 > Columbus, Ohio 43218-2357 > Tel: (614)292-6366 > Fax:614-247-6945 > email: [email protected] ...
Alzheimers disease (AD) is the most prevalent but still incurable neurodegenerative form of dementia. Early diagnosis and intervention are crucial for delaying the onset and progression of the disease. We herein report a novel fluoro-substituted cyanine, F-SLOH, which exhibits good Aβ oligomer selectivity w
Lightning Link Conjugation Kit, 3 x 200ug Reaction . |p|Cyanine dyes are commonly used as fluorescent labels for proteins, nucleic acids and small molecules.
The present invention relates to the use, for the dyeing with lightening effect of human keratin substances, and in particular of human keratin fibres such as the hair, of a composition comprising at least one fluorescent cyanine dye. The present invention allows human keratin substances to be both dyed and at the same time lightened without detriment to said substances. The fluorescent dyes employed in the context of the invention also have high tinctorial affinity for keratin substances and good properties of resistance towards external agents, and especially towards shampoos.
For CF® dye, cyanine dye, FITC, biotin, DNP, and dig Mix-n-Stain™ kits, sodium azide, EDTA, small sugars, and ,10% glycerol have no effect on the labeling. Higher levels of glycerol, or any level of DTT, 2-mercaptoethanol or free amino acids (such as glycine) should be removed using the ultrafiltration vial provided in the kit. See the Product Protocol for more information.. Note that Mix-n-Stain™ Maxi 1 mg Scale Kits, Mix-n-Stain™ Fluorescent Protein Labeling Kits (R-PE, APC, PerCP, or tandem dye), Mix-n-Stain™ Enzyme Labeling Kits (AP, HRP, GOx) have different compatibility requirements and labeling protocols. Check the protocol for your kit to verify that your antibody is compatible with labeling, then choose a kit size that matches your the amount of antibody you wish to label.. Category: Mix-n-Stain™ Antibody Labeling Kits ...
Aiming at the identification of latest fluorescent reporters for qualified optical probes, we assessed the application-applicable functions of the novel asymmetric cyanine, DY-681, compared to the sole clinically permitted dye indocyanine green (ICG), the golden imaging common Cy5.five, as well as the asymmetric cyanine DY-676 effectively exploited by us for the design of various distinction brokers. This comparison included the see analysis in the spectroscopic Houses of your totally free fluorophores and their thermal balance in aqueous Resolution and their cytotoxic potential ...
Wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. To extinguish fire, use water, dry chemical, chemical foam, or alcohol-resistant foam ...
Fluorescent dye derived from Cyanine. Water soluble and the first chlorine atom can be displaced by a nucleophile at 30-35°C and the second at 70-80°C. (U0179) - Products - Abnova
Spare Parts all, peer zada sikandar shah: +923211490351 kia koi dil hae jis par aap qabu pana chahitae hain ? ilm wo jis sae aap ka...
BrightLine Sedat filter set, optimized for DAPI, FITC, TRITC, & Cy5 and other like fluorophores, illuminated with LED-based light engines - DISCONTINUED
PE/Cy7 ®偶联Sca1 / Ly6A/E抗体[D7](ab93537)可与小鼠样本反应并经Flow Cyt实验严格验证,实验条件参看说明书。Abcam对所有产品均提供质保服务,中国75%以上现货。
Discover KIRAVIA Dyes™, a new class of fluorophores with a unique multimer design, helping researchers to expand flow cytometry panel building options.
Definition of CYTOGENIC: producing cells . Variants of CYTOGENIC. cy·to·gen·ic or cy·tog·e·nous \sī-ˈ täj-ə-nəs ...
Read "A new study on solid-state cyanine dye-sensitized solar cells, Research on Chemical Intermediates" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Chapter One highlights the importance of the control of malaria and its significance to the health of the world population. The vital role of the diagnosis of malaria is discussed as are the advantages and disadvantages of the different diagnostic techniques. This Chapter also provides a brief introduction to the chemistry of synthetic dyes. Chapter Two reviews the mechanism of synthesis of cyanine dyes, as these are of particular importance in this research work. The characteristic properties of these dyes are discussed. Chapter Three deals with the spectroscopic properties of the dyes synthesised in this work. Chapter Four studies the use of theoretical calculations for the characterisation of the electronic and structural properties of the dyes as well as the relation of such properties to their biological activity. Chapter Five examines the effectiveness of the biological activity of the dyes as malaria diagnostics. The complex of dye molecule and DNA can be explained in terms of an ...
2. A particle according to claim 1, wherein the hydrophobic dye is represented by the following chemical formula (2): ##STR00014## where: L21, L22, L23, L24, L25, L26, and L27 may be identical to or different from one another and each represent CH or CR27; R27 represents a functional group selected from the group consisting of a halogen atom, an acetoxy group, an amino group, a nitro group, a cyano group, and an alkyl group having 1 to 18 carbon atoms; R27 may form a four-membered ring to a six-membered ring together with an alkyl group bonded to another L21, L22, L23, L24, L25, L26, or L27; X2 represents a counter ion required for neutralizing a charge of a molecule; p2 represents a number of X2s required for neutralizing a charge of an entire molecule; A21 represents any one of structures represented by the following chemical formula (3) and chemical formula (4); and A22 represents any one of structures represented by the following chemical formula (5) and chemical formula (6): ##STR00015## ...
Fluorescent Dye from trimethine cyanine. Water insoluble and forms stable amide bond with primary amines via carbodiimide activation. (U0116) - Products - Abnova
PUR foam exhibits an insignificant level of anisotropic behavior which is shown by the dilatometer measurement. The CTE values are nearly the same in the y- and z-directions between -160°C and 100°C. An additional measurement was performed in each direction; the results also demonstrate the excellent reproducibility of the dilatometer DIL 402 C. ...
Sümeroloji araştırmaları by Tarih, Dil ve Coğrafya Fakültesi (Ankara, Turkey). Sümeroloji Enstitüsü.; 1 edition; First published in 1941
I know that it is common to see a bit of spotting after a pelvic exam, but just wondering if mine is from my exam or an actual show? I am 38 weeks today and had the pelvic 2 days ago to check for dil...
To better illuminate the inner workings of cells, researchers have developed a way to adjust the properties of fluorescent dyes deliberately, resultin
SATIN MAT Lekki Krem – Baza Nawilżająco – matujący Pojemność: 50ml Satin Mat WOMAN Lekki krem, będący jednocześnie doskonałą bazą pod makijaż przeznaczony dla osób od 16 do 40 roku życia z cerą mieszaną, tłustą i normalną. Lekka fomuła…
About this substance This section provides an overview of the calculated volume at which the substance is manufactured or imported to the European Economic Area (EU28 + Iceland, Liechtenstein and Norway). Additionally, if available, information on the use of the substance and how consumers and workers are likely to be exposed to it can also be displayed here.. The use information is displayed per substance life cycle stage (consumer use, in articles, by professional workers (widespread uses), in formulation or re-packing, at industrial sites or in manufacturing). The information is aggregated from the data coming from REACH substance registrations provided by industry.. For a detailed overview on identified uses and environmental releases, please consult the registered substance factsheet.. Use descriptors are adapted from ECHA guidance to improve readability and may not correspond textually to descriptor codes described in Chapter R.12: Use Descriptor system of ECHA Guidance on information ...
Page contains details about cyanine 3-labeled anti-goat IgG microarray on poly(oligo(ethylene glycol) methacrylate-co-glycidyl methacrylate) brush-tethered ZnO nanorod film . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
The use of labelling or staining agents has greatly assisted the study of complex biological interactions in the field of biology. In particular, fluorescent labelling of biomolecules has been demonstrated as an indispensable tool in many biological studies. Types of fluorescent labelling agents that are commonly used include conventional classes of organic fluorophores such as fluorescein and cyanine dyes, as well as newer types of inorganic nanoparticles such as QDs, and novel fluorescent latex/silica nanobeads. The newer classes of fluorescent labels are gaining increasing popularity in place of their predecessors due to their better optical properties such as possessing an enhanced photostability and a larger Stokes shift over conventional organic fluorophores, for example. This paper gives an overview of the recent advances on these luminescent nanomaterials with emphases on their optical characteristics that are crucial in fluorescence microscopy, both advantages and limitations in their ...
In article ,01HAMMGE7PIQ005655 at GW.AGR.CA, BISSETTJ at NCCCOT2.AGR.CA (John Bissett) writes: , An industry client has an urgent need of a permeant vital , stain for Saccharomyces cerevisiae and other yeasts. Their , automated imaging system cannot reliably distinguish yeasts , on grid membrane filters from dust and other , autofluorescing contaminants. The stain should not be , toxic to yeasts. A fluorescent brightener should have an , emission range other than 415-505 nm, the range of , autofluorescence in this application. , , Has anyone knowledge of vital stains/optical brighteners , for this application - or another solution? , , Please address replies to: BISSETTJ at NCCCOT2.AGR.CA , , Thanks , , John Bissett An excellent source for a large variety of dyes is a company called Molecular Probes (Eugene, Oregon). A group of dyes you should consider are the potentiometric cyanine dyes, some of which report the energization of the mitochondrial membrane and which are easily measured with ...
The digital image presented in this section features a cell culture of Indian Muntjac fibroblasts that was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to the cyanine dye, Cy2.
Summary: Pyrvinium Pamoate (PP) (a quinoline-derived cyanine dye) is an relatively non toxic anthelmintic drug, FDA-approved, sold over the counter in countries like Sweden, Norway, Denmark used to treat pinworms in humans and animals. During the recent years this compound has been shown to effectively inhibit various cancer cells types such as brain, colon/colorectal, breast, prostate, etc. ...
In this study, we found a PyA-modified adenine cluster (A-cluster), a minimum fluorescent unit for significant emission wavelength changes, and investigated its photophysical and structural properties. The basic A-cluster unit was an adenine-pentad duplex containing stacked PyA pairs in the center aligned in 2015 Hot Articles in Organic and Biomolecular Chemistry
Send resumes to **[email protected] ,[email protected],/[email protected] ,[email protected],* Position: QTP Tester. Location: Houston, TX. Duration: Long-term. *Need QTP Tester with 5-6 Years Experience, Should have working experience on loadrunner also. * *Thanks,* *Sai* -- You received this message because you are subscribed to the Google Groups REQSRESUMES group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected] To post to this group, send email to [email protected] Visit this group at https://groups.google.com/group/reqsresumes. For more options, visit https://groups.google.com/d/optout ...
Specjalistyczny zabieg rozjaśniający przebarwienia dzięki obecności witaminy C, wyciągów pochodzenia roślinnego (wyciąg z lukrecji, pochodna betaglukanu - wit. E).. ...
Buy Cyanine 3 Bisfunctional MTSEA Dye, Potassium Salt, a thiol reactive fluorescent dye, from Santa Cruz Biotechnology. MF C41H57KN4O12S6, MW 1029.42
Chosen biweekly for their relevance to personal care, Patent Picks are taken from publicly available sources. This edition features patents related to eco-friendly materials and processes.
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So how can this be? One of the most discussed items about Caffenol in general is fog. Caffenol produces always some base fog and it is regarded as something you should avoid if possible. Thats the reason why CCM does not work well with high speed films of 400 ASA boxspeed and faster. The base fog is much too intense, the negs are flat in contrast and the usable speed is more than poor. But with slower speed films it seems to be the reason for the outstanding quality. Fog means that unexposed silver is developed as if it were exposed. But it also means that every silver particle exposed with the smallest amount of light needed for exposure also will be developed. The result is the best shadow detail you can imagine. Most (every?) commercial developers use a fog restraining agent, that will also restrain the low exposed silver developement. By adding some anti-fogging agent to caffenol we would probably end up with an average good developer like so many others. Not so for CCM: without a special ...
So how can this be? One of the most discussed items about Caffenol in general is fog. Caffenol produces always some base fog and it is regarded as something you should avoid if possible. Thats the reason why CCM does not work well with high speed films of 400 ASA boxspeed and faster. The base fog is much too intense, the negs are flat in contrast and the usable speed is more than poor. But with slower speed films it seems to be the reason for the outstanding quality. Fog means that unexposed silver is developed as if it were exposed. But it also means that every silver particle exposed with the smallest amount of light needed for exposure also will be developed. The result is the best shadow detail you can imagine. Most (every?) commercial developers use a fog restraining agent, that will also restrain the low exposed silver developement. By adding some anti-fogging agent to caffenol we would probably end up with an average good developer like so many others. Not so for CCM: without a special ...
Cy3 conjugation / labeling in | 4 hrs with 30 secs hands-on time using Lightning-Link® Cy3 Conjugation Kit (Fast) ab188287. 100% antibody recovery.
Human corneal endothelial cells (HCECs) do not replicate after wounding. Therefore, corneal endothelial deficiency can result in irreversible corneal edema. Descemet stripping automated endothelial keratoplasty (DSAEK) allows selective replacement of the diseased corneal endothelium. However, DSAEK requires a donor cornea and the worldwide shortage of corneas limits its application. This review presents current knowledge on the tissue engineering of corneal endothelium using cultured HCECs. We also provide our recent work on tissue engineering for DSAEK grafts using cultured HCECs. We reconstructed DSAEK grafts by seeding cultured DiI-labelled HCECs on collagen sheets. Then HCEC sheets were transplanted onto the posterior stroma after descemetorhexis in the DSAEK group. Severe stromal edema was detected in the control group, but not in the DSAEK group throughout the observation period. Fluorescein microscopy one month after surgery showed numerous DiI-labelled cells on the posterior corneal surface in
We aimed to test the hypothesis that lateral line placodes form both ampullary organs and neuromasts in cartilaginous fishes, by performing in vivo fate-mapping in the little skate. We used the fluorescent lipophilic dye DiI to label focally the anterodorsal lateral line placode alone, or the anterodorsal plus anteroventral lateral line placodes, in L. erinacea embryos at stage 25 (n=18). These placodes were selected because they are the largest cranial lateral line placodes, hence the easiest to target, and they give rise to both ampullary organs and neuromasts in the axolotl (Northcutt et al., 1995) and paddlefish (Modrell et al., 2011a). The anterodorsal and anteroventral lateral line placodes can both be recognized in skate embryos morphologically as thickenings of ectoderm caudal to the eye and dorsal to the mandibular and hyoid arches, respectively (Fig. 1D), and molecularly, by expression of the gene encoding the transcription co-factor Eya4 (Fig. 1E), an established marker of the ...
Dr. Miquel D. Antoine received her B.S. degree from Spelman College in Chemistry and a M.S. degree in Chemistry from Hampton University in 1991. She joined Dr. Gabor Patonay¹s laboratory at Georgia State University as a research assistant where she investigated the effectiveness of near-infrared cyanine dyes as fluoregenic labels. She began her doctoral studies at Emory University in Atlanta, Georgia, and transferred to the University of Maryland, Baltimore County to work with Dr. Catherine Fenselau, where she received her Ph.D. degree in Analytical Chemistry in 1997. She joined The Johns Hopkins University Applied Physics Laboratory as a post-doctoral fellow in the Spring of 1998 and is currently a member of the Senior Professional Staff in the Milton S. Eisenhower Research and Technology Development Center. Her research interest centers on the use of mass spectrometry in the analysis of biological compounds. Dr. Antoine is a member of the American Chemical Society and the American Society for ...
... carbocyanines MeSH D02.455.326.397.325 --- (4-(m-chlorophenylcarbamoyloxy)-2-butynyl)trimethylammonium chloride MeSH D02.455. ...
Solved in 1919-1921 the problem of the constitutions of the photographic sensitisers known as the isocyanines and carbocyanines ...
Typical slow-response probes include cationic carbocyanines and rhodamines, and ionic oxonols. Commonly used voltage sensitive ...
Carbocyanines DiI, DiS and DiO Derivatives. Indo- (DiI), thia- (DiS) and oxa- (DiO) carbocyanines with short alkyl tails (,7 ... In contrast to cationic carbocyanines, anionic bis-oxonols are largely excluded from mitochondria and are primarily sensitive ...
Effective red sensitizers are the carbocyanines of formula (IX) ##STR20## wherein. each of Z1 and Z2 represents the atoms ... Effective green sensitizers are carbocyanines and cyanines of formulae (X) and (XI) ##STR21## wherein ...
Temperature dependence of fluorescence and photoisomerization in symmetric carbocyanines. Influence of medium viscosity and ...
Typical slow-response probes include cationic carbocyanines and rhodamines, and ionic oxonols. Commonly used voltage sensitive ...
Suitable charge generating materials include thiopyrylium, phthalocyanines, azo pigments, perylenes, carbocyanines, squaraine, ...
In this regard, several perylene compounds and carbocyanines have been shown to interact with G-quadruplex structures. Since ...
Carbocyanines are among the most strongly light-absorbing dyes known and have proven to be useful tools in several different ... Carbocyanines with short alkyl tails attached to the imine nitrogens are employed both as membrane-potential sensors (Slow- ... Like the lipophilic carbocyanines, DiA is commonly used for neuronal membrane tracing (Tracers for Membrane Labeling-Section ... Lipophilic carbocyanines have been used to visualize membrane fusion and cell permeabilization that occurs in response to ...
Multi-colour direct STORM with red emitting carbocyanines. Biol. Cell 104, 229-237 (2012).. ...
carbocyanines and carbocyanine derivatives, e.g., phenylcarbocyanine and hydrox, carbocyanines;. pyridinium salts, e.g., 4-(4- ...
Solved in 1919-1921 the problem of the constitutions of the photographic sensitisers known as the isocyanines and carbocyanines ...
Multi-colour direct STORM with red emitting carbocyanines. Biology of the Cell [http://dx.doi.org/10.1111/boc.201100011 ...
Multi-colour direct STORM with red emitting carbocyanines. Biology of the Cell DOI: 10.1111/boc.201100011 ...
The bleachout dyes useful in the photosystems of this invention are polymethine dyes such as the cyanines, carbocyanines, ... and I, and bleachable polymethine dye selected from the group consisting of cyanines, carbocyanines, merocyanines, styryl dyes ... carbocyanines, merocyanines, styryl dyes and their higher vinylene homologs and wherein said dye is sensitive to light of wave- ... carbocyanines, merocyanines, styryl dyes and their higher vinylene homologs and wherein the solvent for said dye being a non- ...
Animals; Carbocyanines; Female; Fluorescent Dyes; Male (all 7) Animals; Carbocyanines; Female; Fluorescent Dyes; Male; ...
We have used carbocyanine dyes (DiI and DiO) to generate fate maps for the epiblast layer of the chick embryo between stage X and the early primitive streak stage (stages 2-3). The overall distribution of presumptive cell types in these maps is similar to that described for other laboratory species …
Multi-colour direct STORM with red emitting carbocyanines. Biology of the Cell. 104, (4), 229-237 (2012). ...
USPTO has issued the company patent number 7,465,810 for "Reactive 1,3-Crosslinked Carbocyanines and Their Bioconjugates." ... USPTO has issued the company patent number 7,465,810 for "Reactive 1,3-Crosslinked Carbocyanines and Their Bioconjugates." ... USPTO has issued the company patent number 7,465,810 for "Reactive 1,3-Crosslinked Carbocyanines and Their Bioconjugates." ...
Other dyes (rhodamines, carbocyanines, etc.) can be used, but laser 102, beamsplitters 108 and 106, and filters 138 and 124 may ...
The photosensitizers belong to one of the following categories: ketones and their derivatives, carbocyanines and methines, ...
... a family of nanovesicles proposed as scaffolds for the nanostructuration of commercial lipophilic carbocyanines ... ...
Here, we explored the mechanistic properties of a specific group of NIR heptamethine carbocyanines including MHI-148 dye we ...
... carbocyanines, hemicyanines, etc. Photographic systems in which the above sensitizing dyes can be used include a facsimile ...
... carbocyanines, Phenoxazone, Carbazine, oxazines, and rhodamines insbesonde re are suitable, but preferably those dyes (600-1000 ...
... a family of nanovesicles proposed as scaffolds for the nanostructuration of commercial lipophilic carbocyanines (1,1- ...
keywords = "Adenosine Triphosphate, Benzenesulfonates, Binding Sites, Carbocyanines, Dose-Response Relationship, Drug, ...