Virus Assembly
Virion
Cryoelectron Microscopy
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Dependovirus
Viral Structural Proteins
Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).
Amino Acid Sequence
Virus Uncoating
Virus Replication
Herpesvirus 1, Human
The type species of SIMPLEXVIRUS causing most forms of non-genital herpes simplex in humans. Primary infection occurs mainly in infants and young children and then the virus becomes latent in the dorsal root ganglion. It then is periodically reactivated throughout life causing mostly benign conditions.
Models, Molecular
Parvovirus, Canine
Poliovirus
A species of ENTEROVIRUS which is the causal agent of POLIOMYELITIS in humans. Three serotypes (strains) exist. Transmission is by the fecal-oral route, pharyngeal secretions, or mechanical vector (flies). Vaccines with both inactivated and live attenuated virus have proven effective in immunizing against the infection.
DNA Packaging
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Calicivirus, Feline
Parvovirus
Spodoptera
Baculoviridae
Family of INSECT VIRUSES containing two subfamilies: Eubaculovirinae (occluded baculoviruses) and Nudibaculovirinae (nonoccluded baculoviruses). The Eubaculovirinae, which contain polyhedron-shaped inclusion bodies, have two genera: NUCLEOPOLYHEDROVIRUS and GRANULOVIRUS. Baculovirus vectors are used for expression of foreign genes in insects.
Caliciviridae Infections
Norovirus
Norwalk virus
The type species in the genus NOROVIRUS, first isolated in 1968 from the stools of school children in Norwalk, Ohio, who were suffering from GASTROENTERITIS. The virions are non-enveloped spherical particles containing a single protein. Multiple strains are named after the places where outbreaks have occurred.
Cyclophilin A
Base Sequence
Cercopithecus aethiops
Vero Cells
Genetic Vectors
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Caliciviridae
A family of RNA viruses infecting a broad range of animals. Most individual species are restricted to their natural hosts. They possess a characteristic six-pointed starlike shape whose surfaces have cup-shaped (chalice) indentions. Transmission is by contaminated food, water, fomites, and occasionally aerosolization of secretions. Genera include LAGOVIRUS; NORWALK-LIKE VIRUSES; SAPPORO-LIKE VIRUSES; and VESIVIRUS.
Reoviridae
Minute virus of mice
Mutation
Parvoviridae
Gene Products, gag
Proteins coded by the retroviral gag gene. The products are usually synthesized as protein precursors or POLYPROTEINS, which are then cleaved by viral proteases to yield the final products. Many of the final products are associated with the nucleoprotein core of the virion. gag is short for group-specific antigen.
Levivirus
Picornaviridae
Bromovirus
Open Reading Frames
Nucleocapsid
Aphthovirus
Neutralization Tests
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Polyomavirus
Rotavirus
HeLa Cells
Rous sarcoma virus
Protein Conformation
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Receptors, Virus
Mason-Pfizer monkey virus
Enterovirus B, Human
HIV-1
Parvovirus B19, Human
Microscopy, Electron, Transmission
Negative Staining
The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
Recombinant Fusion Proteins
Amino Acid Substitution
The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.
Bacteriophage P22
Nodaviridae
Rubella virus
Gene Expression Regulation, Viral
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Hemorrhagic Disease Virus, Rabbit
Virus Internalization
Virus Release
Feline panleukopenia virus
A species of PARVOVIRUS infecting cats with a highly contagious enteric disease. Host range variants include mink enteritis virus, canine parvovirus (PARVOVIRUS, CANINE), and raccoon parvovirus. After infecting their new hosts, many of these viruses have further evolved and are now considered distinct species.
Infectious bursal disease virus
Viral Vaccines
Rhinovirus
Viral Core Proteins
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Papillomaviridae
A family of small, non-enveloped DNA viruses infecting birds and most mammals, especially humans. They are grouped into multiple genera, but the viruses are highly host-species specific and tissue-restricted. They are commonly divided into hundreds of papillomavirus "types", each with specific gene function and gene control regions, despite sequence homology. Human papillomaviruses are found in the genera ALPHAPAPILLOMAVIRUS; BETAPAPILLOMAVIRUS; GAMMAPAPILLOMAVIRUS; and MUPAPILLOMAVIRUS.
Bacteriophage T4
Virosomes
Semi-synthetic complex derived from nucleic-acid free viral particles. They are essentially reconstituted viral coats, where the infectious nucleocapsid is replaced by a compound of choice. Virosomes retain their fusogenic activity and thus deliver the incorporated compound (antigens, drugs, genes) inside the target cell. They can be used for vaccines (VACCINES, VIROSOME), drug delivery, or gene transfer.
Oncogene Proteins, Viral
Protein Structure, Quaternary
Sindbis Virus
The type species of ALPHAVIRUS normally transmitted to birds by CULEX mosquitoes in Egypt, South Africa, India, Malaya, the Philippines, and Australia. It may be associated with fever in humans. Serotypes (differing by less than 17% in nucleotide sequence) include Babanki, Kyzylagach, and Ockelbo viruses.
Transduction, Genetic
Bacteriophage P2
Protein Binding
Centrifugation, Density Gradient
Orthoreovirus, Mammalian
Mamastrovirus
Herpesvirus 1, Suid
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Protein Multimerization
Crystallography, X-Ray
Bacteriophage PRD1
Cricetinae
Circovirus
A genus of the family CIRCOVIRIDAE that infects SWINE; PSITTACINES; and non-psittacine BIRDS. Species include Beak and feather disease virus causing a fatal disease in psittacine birds, and Porcine circovirus causing postweaning multisystemic wasting syndrome in pigs (PORCINE POSTWEANING MULTISYSTEMIC WASTING SYNDROME).
Hepatitis B virus
The type species of the genus ORTHOHEPADNAVIRUS which causes human HEPATITIS B and is also apparently a causal agent in human HEPATOCELLULAR CARCINOMA. The Dane particle is an intact hepatitis virion, named after its discoverer. Non-infectious spherical and tubular particles are also seen in the serum.
Virus Attachment
CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts. (1/5649)
Primary fibroblasts are not efficiently transduced by subgroup C adenovirus (Ad) vectors because they express low levels of the high-affinity Coxsackie virus and adenovirus receptor (CAR). In the present study, we have used primary human dermal fibroblasts as a model to explore strategies by which Ad vectors can be designed to enter cells deficient in CAR. Using an Ad vector expressing the human CAR cDNA (AdCAR) at high multiplicity of infection, primary fibroblasts were converted from being CAR deficient to CAR sufficient. Efficiency of subsequent gene transfer by standard Ad5-based vectors and Ad5-based vectors with alterations in penton and fiber was evaluated. Marked enhancement of binding and transgene expression by standard Ad5 vectors was achieved in CAR-sufficient fibroblasts. Expression by AdDeltaRGDbetagal, an Ad5-based vector lacking the arginine-glycine-aspartate (RGD) alphaV integrin recognition site from its penton base, was achieved in CAR-sufficient, but not CAR-deficient, cells. Fiber-altered Ad5-based vectors, including (a) AdF(pK7)betagal (bearing seven lysines on the end of fiber) (b) AdF(RGD)betagal (bearing a high-affinity RGD sequence on the end of fiber), and (c) AdF9sK betagal (bearing a short fiber and Ad9 knob), demonstrated enhanced gene transfer in CAR-deficient fibroblasts, with no further enhancement in CAR-sufficient fibroblasts. Together, these observations demonstrate that CAR deficiency on Ad targets can be circumvented either by supplying CAR or by modifying the Ad fiber to bind to other cell-surface receptors. (+info)Maturation-induced conformational changes of HIV-1 capsid protein and identification of two high affinity sites for cyclophilins in the C-terminal domain. (2/5649)
Viral incorporation of cyclophilin A (CyPA) during the assembly of human immunodeficiency virus type-1 (HIV-1) is crucial for efficient viral replication. CyPA binds to the previously identified Gly-Pro90 site of the capsid protein p24, but its role remained unclear. Here we report two new interaction sites between cyclophilins and p24. Both are located in the C-terminal domain of p24 around Gly-Pro157 and Gly-Pro224. Peptides corresponding to these regions showed higher affinities (Kd approximately 0.3 microM) for both CyPA and cyclophilin B than the best peptide derived from the Gly-Pro90 site ( approximately 8 microM) and thus revealed new sequence motifs flanking Gly-Pro that are important for tight interaction of peptide ligands with cyclophilins. Between CyPA and an immature (unprocessed) form of p24, a Kd of approximately 8 microM was measured, which corresponded with the Kd of the best of the Gly-Pro90 peptides, indicating an association via this site. Processing of immature p24 by the viral protease, yielding mature p24, elicited a conformational change in its C-terminal domain that was signaled by the covalently attached fluorescence label acrylodan. Consequently, CyPA and cyclophilin B bound with much higher affinities ( approximately 0.6 and 0.25 microM) to the new, i.e. maturation-generated sites. Since this domain is essential for p24 oligomerization and capsid cone formation, CyPA bound to the new sites might impair the regularity of the capsid cone and thus facilitate in vivo core disassembly after host infection. (+info)Low temperature and pressure stability of picornaviruses: implications for virus uncoating. (3/5649)
The family Picornaviridae includes several viruses of great economic and medical importance. Poliovirus replicates in the human digestive tract, causing disease that may range in severity from a mild infection to a fatal paralysis. The human rhinovirus is the most important etiologic agent of the common cold in adults and children. Foot-and-mouth disease virus (FMDV) causes one of the most economically important diseases in cattle. These viruses have in common a capsid structure composed of 60 copies of four different proteins, VP1 to VP4, and their 3D structures show similar general features. In this study we describe the differences in stability against high pressure and cold denaturation of these viruses. Both poliovirus and rhinovirus are stable to high pressure at room temperature, because pressures up to 2.4 kbar are not enough to promote viral disassembly and inactivation. Within the same pressure range, FMDV particles are dramatically affected by pressure, with a loss of infectivity of more than 4 log units observed. The dissociation of polio and rhino viruses can be observed only under pressure (2.4 kbar) at low temperatures in the presence of subdenaturing concentrations of urea (1-2 M). The pressure and low temperature data reveal clear differences in stability among the three picornaviruses, FMDV being the most sensitive, polio being the most resistant, and rhino having intermediate stability. Whereas rhino and poliovirus differ little in stability (less than 10 kcal/mol at 0 degrees C), the difference in free energy between these two viruses and FMDV was remarkable (more than 200 kcal/mol of particle). These differences are crucial to understanding the different factors that control the assembly and disassembly of the virus particles during their life cycle. The inactivation of these viruses by pressure (combined or not with low temperature) has potential as a method for producing vaccines. (+info)Time-resolved fluorescence investigation of the human immunodeficiency virus type 1 nucleocapsid protein: influence of the binding of nucleic acids. (4/5649)
Depending on the HIV-1 isolate, MN or BH10, the nucleocapsid protein, NCp7, corresponds to a 55- or 71-amino acid length product, respectively. The MN NCp7 contains a single Trp residue at position 37 in the distal zinc finger motif, and the BH10 NCp7 contains an additional Trp, at position 61 in the C-terminal chain. The time-resolved intensity decay parameters of the zinc-saturated BH10 NCp7 were determined and compared to those of single-Trp-containing derivatives. The fluorescence decay of BH10 NCp7 could be clearly represented as a linear combination (with respect to both lifetimes and fractional intensities) of the individual emitting Trp residues. This suggested the absence of interactions between the two Trp residues, a feature that was confirmed by molecular modeling and fluorescence energy transfer studies. In the presence of tRNAPhe, taken as a RNA model, the same conclusions hold true despite the large fluorescence decrease induced by the binding of tRNAPhe. Indeed, the fluorescence of Trp37 appears almost fully quenched, in keeping with a stacking of this residue with the bases of tRNAPhe. Despite the multiple binding sites in tRNAPhe, the large prevalence of ultrashort lifetimes, associated with the stacking of Trp37, suggests that this stacking constitutes a major feature in the binding process of NCp7 to nucleic acids. In contrast, Trp61 only stacked to a small extent with tRNAPhe. The behavior of this residue in the tRNAPhe-NCp7 complexes appeared to be rather heterogeneous, suggesting that it does not constitute a major determinant in the binding process. Finally, our data suggested that the binding of NCp7 proteins from the two HIV-1 strains to nonspecific nucleic acid sequences was largely similar. (+info)The haplotype distribution of two genes of citrus tristeza virus is altered after host change or aphid transmission. (5/5649)
Genetic variability of citrus tristeza virus (CTV) was studied using the haplotypes detected by single-strand conformation polymorphism (SSCP) analysis of genes p18 and p20 in six virus populations of two origins. The Spanish group included a CTV isolate and subisolates obtained by graft-transmission to different host species. The other included two subisolates aphid-transmitted from a single Japanese isolate. The homozygosity observed for gene p20 was always significantly higher than that expected under neutral evolution, whereas only three populations showed high homozygosity for p18, suggesting stronger host constraints for p20 than for p18. Sequential transmissions of a Spanish isolate to new host species increased the difference between its population and that of the successive subisolates for gene p18, as estimated by the F statistic. Analysis of molecular variance indicated that variation between both groups of populations was not statistically significant, whereas variations between populations of the same group or within populations were significant for both genes studied. Our data indicate that selection affects the haplotype distribution and that adaptation to a new host can be as important or more as the geographical origin. Variation of the CTV populations after host change or aphid transmission may explain in part the wide biological variability observed among CTV isolates. (+info)A new picornavirus isolated from bank voles (Clethrionomys glareolus). (6/5649)
A previously unknown picornavirus was isolated from bank voles (Clethrionomys glareolus). Electron microscopy images and sequence data of the prototype isolate, named Ljungan virus, showed that it is a picornavirus. The amino acid sequences of predicted Ljungan virus capsid proteins VP2 and VP3 were closely related to the human pathogen echovirus 22 (approximately 70% similarity). A partial 5' noncoding region sequence of Ljungan virus showed the highest degree of relatedness to cardioviruses. Two additional isolates were serologically and molecularly related to the prototype. (+info)The cleavable carboxyl-terminus of the small coat protein of cowpea mosaic virus is involved in RNA encapsidation. (7/5649)
The site of cleavage of the small coat protein of cowpea mosaic virus has been precisely mapped and the proteolysis has been shown to result in the loss of 24 amino acids from the carboxyl-terminus of the protein. A series of premature termination and deletion mutants was constructed to investigate the role or roles of these carboxyl-terminal amino acids in the viral replication cycle. Mutants containing premature termination codons at or downstream of the cleavage site were viable but reverted to wild-type after a single passage through cowpea plants, indicating that the carboxyl-terminal amino acids are important. Mutants with the equivalent deletions were genetically stable and shown to be debilitated with respect to virus accumulation. The specific infectivity of preparations of a deletion mutant (DM4) lacking all 24 amino acids was 6-fold less than that of a wild-type preparation. This was shown to be a result of DM4 preparations containing a much increased percentage (73%) of empty (RNA-free) particles, a finding that implicates the cleavable carboxyl-terminal residues in the packaging of the virion RNAs. (+info)Induction of autoantibodies to mouse CCR5 with recombinant papillomavirus particles. (8/5649)
The vertebrate immune system has evolved to respond vigorously to microbial infection but to ignore self-antigens. Evidence has emerged that B cell responses to viruses are initiated by immune recognition of ordered arrays of antigen on the viral surface. To test whether autoantibodies against a self-antigen can be induced by placing it in a context that mimics the ordered surface of a viral particle, a peptide representing an extracellular loop of the mouse chemokine receptor CCR5 was incorporated into an immunodominant site of the bovine papillomavirus virus L1 coat protein, which self-assembles into virus-like particles. Mice inoculated with chimeric L1-CCR5 particles generated autoantibodies that bound to native mouse CCR5, inhibited binding of its ligand RANTES, and blocked HIV-1 infection of an indicator cell line expressing a human-mouse CCR5 chimera. These results suggest a general method for inducing autoantibodies against self-antigens, with diverse potential basic research and clinical applications. (+info)
Herpes simplex virus capsids are transported in neuronal axons without an envelope containing the viral glycoproteins<...
Control of capsid disassembly is crucial for efficient HIV-1 cDNA activity
Extent of protein-protein interactions and quasi-equivalence in viral capsids
Major capsid reinforcement by a minor protein in herpesviruses and pha by Udom Sae-Ueng, Ting Liu et al.
Herpes simplex virus capsids assembled in insect cells infected with recombinant baculoviruses: structural authenticity and...
Assembly and Structure of Bacteriophage Capsids and Tails - [email protected]
Atomic structure of the human herpesvirus 6B capsid and capsid-associated tegument complexes | Nature Communications
The outer shell of the adenovirus capsid comprises three major types - BET-bromodomain inhibition research
Genome polyprotein - Coxsackievirus A24 (strain EH24/70)
Structural insights into the assembly and regulation of distinct viral capsid complexes
An HIV-1 capsid binding protein TRIM11 accelerates viral uncoating | Retrovirology | Full Text
Proteasomal Degradation of Herpes Simplex Virus Capsids in Macrophages Releases DNA to the Cytosol for Recognition by DNA...
Investigating factors that affect HIV-1 capsid stability by Max Mao and Joshua Temple
Systemic delivery of siRNA by chimeric capsid protein: Tumor targeting and RNAi activity in vivo<...
Intermediate capsid protein VP6 - Rotavirus A (strain RVA/SA11-Both/G3P5B[2]) (RV-A)
The study of the mechanism of formation of HIV capsid can be the basis for developing a new type of therapy - Life4me+
Rabbit Polyclonal to NCBP2. | healthweblognews.info
Origin of unenveloped capsids in the cytoplasm of cells infected with herpes simplex virus 1. | Journal of Virology
Capsid labelled HIV to investigate the role of capsid during nuclear import and integration - Rocha Lab
Nuclear Herpesvirus Capsid Motility Is Not Dependent on F-Actin | mBio
Retrovirus capsid dimerization domain-like alignments
Permeability of the HIV-1 capsid to metabolites modulates viral DNA synthesis | Biophysics Lab
Institute Of Molecular Biophysics | Master Calendar
Common green capsid (Lygocoris pabulinus (Linnaeus)) - Apple Best Practice Guide
1gwp - Proteopedia, life in 3D
Structural convergence between Cryo-EM and NMR reveals intersubunit interactions critical for HIV-1 capsid function. - Nuffield...
Machine-guided engineering of AAV capsids | Beer Reviewed
Icosahedral virus capsid, illustration - Stock Image F018/4295 - Science Photo Library
FCGR3A - BET-bromodomain inhibition research
The previously characterized monoclonal antibodies (MAbs) A1, A69, B1, and A20 - Novel targets for Alzheimers disease treatment
neverendingbooks
Universitätsklinikum Heidelberg: AG Urban
Immune Response
Capsid titer quantification for AAV-based therapeutics
Herpesviruses remodel host membranes for virus egress<...
DCV capsid polyprotein抗体|Abcam中国|Anti-DCV capsid polyprotein抗体
Capsid analysis
Capsid bugs - Which? Gardening Helpdesk
EMDB-6037: Capsid Expansion Mechanism Of Bacteriophage T7 Revealed By Multi-... - Yorodumi
Electrostatic theory of viral self-assembly: a toy model - CBGSE
Capsid proteins self-assemble to form stable shell : Viruses - AskNature
More-powerful virus inhibitors from structure-based analysis of HEV71 capsid-binding molecules - CAMS Oxford Institute
Phage lytic cycle
Theoretical Condensed Matter | Gelbart Lab
single-molecule biophysics | Gelbart Lab
Mathematical models for tubular structures in the family of Papovaviridae - White Rose Research Online
Maturation | Technology Trends
In vitro protease cleavage and computer simulations reveal the HIV-1 capsid maturation pathway - Wellcome Centre for Human...
Optimization of an Elastic Network Augmented Coarse Grained Model to Study CCMV Capsid Deformation - pdf descargar
NR 3.0 59619.1
Display of a PorA peptide from Neisseria meningitidis on the bacteriophage T4 capsid surface. | Infection and Immunity
The herpesvirus capsid surface protein, VP26, and the majority of the tegument proteins are dispensable for capsid transport...
Mature HIV-1 capsid structure by cryo-electron microscopy and all-atom molecular dynamics. - Nuffield Department of Medicine
Post-translational modifications in capsid proteins of recombinant adeno-associated virus (AAV) 1-rh10 serotypes [Mass...
Identification of a short, highly conserved, motif required for picornavirus capsid precursor processing at distal sites
A protein with simultaneous capsid scaffolding and dsRNA-binding activities enhances the birnavirus capsid mechanical stability...
Herpesvirus capsid protein - Stock Image F006/9435 - Science Photo Library
The Structure of the Rotavirus Inner Capsid Studied by Electron Microscopy of Chemically Disrupted Particles | Microbiology...
The herpesvirus VP1/2 protein is an effector of dynein-mediated capsid transport and neuroinvasion<...
Topologically Linked Protein Rings in the Bacteriophage HK97 Capsid | Science
Partial amino-terminal sequences of the polyoma nonhistone proteins VP1, VP2, and VP3 synthesized in vitro<...
Metabolic biotinylation of the adenoviral capsid: Avidin-based applications and studies of ligand-targeted gene delivery
Permalien vers TRIM5 is an innate immune sensor for the retrovirus capsid lattice
Immunoinformatics Approach for Designing Epitope-Based Peptides Vaccine of L1 Major Capsid Protein against HPV Type 16 - IJMCR
Self-assembly of hexahistidine-tagged tobacco etch virus capsid protein into microfilaments that induce IgG2-specific response...
Mutagenesis of N-terminal residues of feline foamy virus Gag reveals entirely distinct functions during capsid formation,...
JCI -
Unique region of the minor capsid protein of human parvovirus B19 is exposed on the virion surface.
Samuel K-Campos | BIO5
EMDB-1710: Cryo-EM 3D model of the icosahedral particle composed of Rous sar... - Yorodumi
Identification of Critical Amino Acid in Capsid Protein of PCV2 | The Pig Site
PRIME PubMed | High prevalence of immunoglobulin A antibody against Epstein-Barr virus capsid antigen in adult patients with...
Team:Heidelberg/Project/Capsid Shuffling/Homology Based - 2010.igem.org
Role of RNA Branchedness in the Competition for Viral Capsid Proteins | Avinoam Ben-Shaul
The individual papillomavirus type 11 (HPV-11) L1 main capsid protein could - HIV Subtypes B and C gp120 and Methamphetamine...
British Library EThOS: Interactions of retroviral capsid proteins with restriction factors
Recent advances in the development of HBV capsid assembly modulators
Subgenomic capsid protein elisa and antibody
Outer capsid glycoprotein elisa and antibody
RVA infections induce neutralizing Ab muscles in opposition to outer capsid proteins VP4 and VP7 | IDH Inhibitor idhinhibitor...
Papillomavirus capsid protein expression in Escherichia coli: purification and assembly of HPV11 and HPV16 L1
What Additional Protective Layer Of Protein Surrounds Some Viruses - gabrieldroberts.com
EBV Antibody To Viral Capsid Antigen, IgM
EBV Antibody to Viral Capsid Antigen, IgM Test
Annotation rule MF 04003
Birkbeck Institutional Research Online
Team:Heidelberg/Project/Capsid Shuffling/ViroBytes - 2010.igem.org
The refined structure of a protein catenane: the HK97 bacteriophage capsid at 3.44 Å resolution
Insight into the mechanisms of adenovirus capsid disassembly from studies of defensin neutralization
Herpes Simplex Virus Infections | Harrisons Principles of Internal Medicine, 19e | AccessMedicine | McGraw-Hill Medical
Order - Herpesvirales - Institut Pasteur
Scientific Protocols -
Selection of Phage-displayed peptides that bind to cancer following treatment
AAV Capsid Engineering
Capsid
The proteins making up the capsid are called capsid proteins or viral coat proteins (VCP). The capsid and inner genome is ... rotavirus and bacteriophage φ6 have capsids built of 120 copies of capsid protein, corresponding to a T = 2 capsid, or arguably ... The capsid faces may consist of one or more proteins. For example, the foot-and-mouth disease virus capsid has faces consisting ... Capsids are broadly classified according to their structure. The majority of the viruses have capsids with either helical or ...
P24 capsid protein
p24 is a component of the HIV particle capsid. There are approximately 2000 molecules per virus particle, or at a molecule ...
HIV capsid inhibition
In the management of HIV/AIDS, HIV capsid inhibitors are antiretroviral medicines that target the capsid shell of the virus. ... This bond stabilizes the capsid structure and inhibits the functional disassembly of the capsid in infected cells. Inhibition ... The uncoating process is a highly ordered multistep process in which the capsid is weakened and most or all capsid proteins are ... and also inhibits the production of new capsid shells within the cell. Ebselen was identified as a capsid inhibitor using a ...
HHV capsid portal protein
The Herpes viral DNA enters and exits the capsid via the capsid portal. The capsid portal is formed by twelve copies of portal ... HHV Capsid Portal Protein, or HSV-1 UL-6 protein, is the protein which forms a cylindrical portal in the capsid of Herpes ... When the capsid is nearly complete, the viral DNA enters the capsid (i.e., the DNA is encapsidated) by a mechanism involving ... Each icosahedral capsid contains a single portal, located in one vertex. The portal is formed during initial capsid assembly ...
Major capsid protein VP1
... is a viral protein that is the main component of the polyomavirus capsid. VP1 monomers are generally ... The capsid contains three proteins; VP1 is the primary component and forms a 360-unit outer capsid layer composed of 72 ... All of the capsid proteins are expressed from the late region of the viral genome, so named because expression occurs only late ... VP2 and VP3 assemble inside the capsid in contact with VP1, with a stoichiometry of one VP2 or VP3 molecule to each pentamer.: ...
Flavivirus capsid hairpin cHP
The Flavivirus capsid hairpin cHP is a conserved RNA hairpin structure identified within the capsid coding region of several ... the first of which is the capsid protein. The cHP hairpin is located within the capsid coding region between two AUG start ... Page for flavivirus capsid hairpin cHP at Rfam v t e (Cis-regulatory RNA elements, Flaviviruses, All stub articles, Molecular ...
Minor capsid proteins VP2 and VP3
Minor capsid protein VP2 and minor capsid protein VP3 are viral proteins that are components of the polyomavirus capsid. ... Polyomavirus capsids are composed of three proteins; the major component is major capsid protein VP1, which self-assembles into ... The minor components are VP2 and VP3, which bind in the interior of the capsid. All three capsid proteins are expressed from ... April 2006). "The VP2/VP3 minor capsid protein of simian virus 40 promotes the in vitro assembly of the major capsid protein ...
Epstein-Barr virus viral-capsid antigen
The Epstein-Barr virus viral-capsid antigen (EBV-VCA) is the viral protein that forms the viral capsid of the Epstein-Barr ... It is the antigen targeted by antibodies that bond to viral capsid antigens (VCA). Such antibodies can be used in serology to ...
John Walter (artist)
Capsid (2018), published by HOME Publications Shonky: The Aesthetics of Awkwardness (2018), published by Hayward Publishing ... "John Walter, CAPSID". Retrieved 20 November 2018. "Shonky: The Aesthetics of Awkwardness". Retrieved 20 November 2018. "Coming ... visual culture and HIV through his multimedia project Alien Sex Club and the behaviour of viruses through his project CAPSID ( ...
Joseph Osmundson
His 2016 book Capsid: A Love Song won the POZ Award for best HIV writing (fiction/poetry) and was a finalist for a Lambda ... "Capsid: A Love Song". Los Angeles Review of Books. Retrieved 2018-06-06. Osmundson, Joseph. "Things Unsaid". Gawker. Retrieved ... Capsid: A Love Song (Indolent Books, 2016), ISBN 978-1-945023-03-3 INSIDE/OUT (Sibling Rivalry Press, 2018), ISBN 978-1-943977- ...
Closterotomus norwegicus
"Potato capsid Closterotomus norwegicus". Photos of Insects in Cambridge. Edkins Family Index Page. Archived from the original ... Closterotomus norvegicus (also known as the potato capsid) is a species of bugs belonging to the family Miridae, subfamily ...
Endornaviridae
Dolja, Valerian V (2001). "Capsid-Less RNA Viruses". eLS. doi:10.1002/9780470015902.a0023269. ISBN 978-0470016176. ICTVdB ...
Fiji disease virus
The capsid has two layers and is icosahedral. The two known cell surface proteins are A type and B type spikes. The capsid ...
Structure and genome of HIV
... capsid protein, p24); SP1 (spacer peptide 1, p2); NC (nucleocapsid protein, p7); SP2 (spacer peptide 2, p1) and P6 protein. pol ... Each virion comprises a viral envelope and associated matrix enclosing a capsid, which itself encloses two copies of the single ... positive-sense single-stranded RNA enclosed by a conical capsid composed of the viral protein p24, typical of lentiviruses. The ...
Narnavirus
They do not have structural proteins or a capsid. Narnaviruses have nonsegmented, linear, positive-sense, single-stranded RNA ... Dolja, V. V.; Koonin, E. V. (2012). "Capsid-Less RNA Viruses". ELS. doi:10.1002/9780470015902.a0023269. ISBN 978-0470016176. ...
Entebbe bat virus
The capsid protein is enveloped. The genomic arrangement is a linear (+)ssRNA. Its genomic segmentation is Monopartite (Flint ... The genome encodes 3 structural proteins (Capsid, prM, and Envelope) and 8 non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A ... Capsid, prM, and Envelope) and 8 non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, NS5 and NS5B). The genomic RNA is ...
Murine polyomavirus
The capsid contains three proteins; capsid protein VP1 is the primary component and self-assembles into a 360-unit outer capsid ... MPyV capsid protein VP1 binds to sialic acids of gangliosides GD1a and GT1b on the cell surface. The functions of VP2 and VP3 ... Capsid proteins, produced in the cytoplasm of the host cell, enter the nucleus as assembled capsomers consisting of pentameric ... The three genes in the late region express the three capsid proteins VP1, VP2, and VP3. Between the early and late regions is a ...
Tectivirus
The capsid has two layers. The outer layer is a protein structure of 240 capsid proteins trimers, and the inner one is a ... mobile arms and membrane-capsid interactions in the bacteriophage PRD1 capsid". Nature Structural Biology. 9 (10): 756-63. doi: ... Capsid proteins polymerize around a lipoprotein vesicle translocated in the cytoplasm by virion assembly factors. Mature virons ...
Vesicular exanthema of swine virus
The isometric capsid has a diameter of 35-39 nm. Capsids appear round to hexagonal in outline. The capsid surface structure ... Virions consist of a capsid. Virus capsid is not enveloped, round with icosahedral symmetry. ... Capsid with 32 cup-shaped depressions. Under in vitro conditions, virions are inactivated in acid environment of pH 3-5. The ...
Vesivirus
Virus capsid is non-enveloped, and are round with icosahedral symmetry and triangulation number T=3. The isometric capsid has a ... Viral structural protein: Capsid protein has a molar mass of 58000-60000 Da; is the coat protein. Capsid protein has a ... Empty virions have diameter of 23 nm, with triangulation number T=1. The capsid surface structure reveals a regular pattern ... "Calicivirus isolate Geel 2008/Belgium non-structural polyprotein gene, partial cds; and capsid protein precursor and small ...
Petuvirus
Its capsid shells are multilayered. The capsid is round to elongate with icosahedral symmetry and the virus is composed of 16% ...
Astroviridae
Capsids that are made of the VP70 protein are cleaved by trypsin to make particles that are very infectious (VP25/26, VP27/29 ... Following encapsidation, immature capsids are released from the cell without lysis. Extracellular virions are cleaved by ... They have spherical shapes and consist of a capsid protein shell. Astroviruses have distinctive five or six pointed star-like ... Arias, Carlos F.; DuBois, Rebecca M. (19 January 2017). "The Astrovirus Capsid: A Review". Viruses. 9 (1): 15. doi:10.3390/ ...
Alfalfa mosaic virus
The virion has a capsid (coat protein) but no envelope. The icosahedral symmetry of the capsid is round to elongated. The range ... The capsid protein remains attached to the coat protein binding site (CPB) at the 3'- end of the RNAs. The initiation factors ... The capsid dissociates from CPB. CPB undergoes a conformational change into TLS (tRNA-like structure). P1/P2 bind to the minus ... For example, DNA derived from AMV encoding the gene for the capsid has been inserted into alfalfa plants. This reduced the ...
Ranid herpesvirus 1
The capsid itself has been measured to be approximately 90-110 nm in diameter and expresses T=16 icosahedral symmetry. The ... The entire capsid contains 162 capsomeres. Other viruses within the family Alloherpesviridae have been shown to have spherical ... The steps to this method of replication (excluding the aforementioned process of cell entry) are as follow: The capsid is ... which are involved in the formation and structure of the capsid. These late proteins are transported back into the nucleus. The ...
Asparagus virus 1
The virus capsid is not enveloped. Capsid / nucleocapsid is elongated with helical symmetry. The capsid is filamentous, ...
Gaussian network model
Rader, AJ; Vlad, Daniel; Bahar, Ivet (2005). "Maturation Dynamics of Bacteriophage HK97 Capsid". Structure. 13 (3): 413-21. doi ... to large macromolecular assemblies such as a ribosome or a viral capsid. Protein domain dynamics plays key roles in a multitude ...
Gyrovirus
VP1 is the 51kd capsid protein; in addition to its structural function, it also contains motifs for rolling circle replication ... They are nonenveloped and have an icosahedral capsid with T=1 symmetry. The unique, single protein, trumpet-shaped capsomeres ... of Gyrovirus are arranged into 12 pentomers yielding a capsid 60 units in size. The genome is circular, non-segmented, and 2290 ...
Stephen C. Harrison
Modis Y, Trus BL, Harrison SC (September 2002). "Atomic model of the papillomavirus capsid". EMBO J. 21 (18): 4754-62. doi: ... including the capsid of human papillomavirus, the envelope of dengue virus, and several components of HIV. He has also turned ...
Replicon (genetics)
... that lack a capsid. DNA replication Origin of replication Secondary chromosome Watson JD (2014). Molecular Biology of the Gene ...
Bovine papillomavirus
The capsid is formed of the L1 and L2 structural proteins, with the L1 C-terminus exposed. All BPVs have a circular double- ... ISBN 978-1-904455-04-2. Modis Y, Trus BL, Harrison SC (2002). "Atomic model of the papillomavirus capsid". EMBO J. 21 (18): ... Like other papillomaviruses, BPVs are small non-enveloped viruses with an icosahedral capsid around 50-60 nm in diameter. ...
Backbone structure of the infectious ε15 virus capsid revealed by electron cryomicroscopy | Nature
Here we report the 4.5-Å resolution structure of a 22-MDa macromolecular assembly, the capsid of the infectious epsilon15 (ε15 ... From this density map we constructed a complete backbone trace of its major capsid protein, gene product 7 (gp7). The structure ... Its structure, location and high binding affinity to the capsid indicate that the gp10 dimer functions as a molecular staple ... Novel fold and capsid-binding properties of the lambda-phage display platform protein gpD. Nature Struct. Biol. 7, 230-237 ( ...
RCSB PDB - 4HBE: Crystal Structure of Rubella virus capsid protein (residues 127-277)
Crystal Structure of Rubella virus capsid protein (residues 127-277) ... Capsid protein. A, B. 151. Rubella virus strain M33. Mutation(s): 0 Gene Names: Capsid. ... Rubella virus capsid protein structure and its role in virus assembly and infection.. Mangala Prasad, V., Willows, S.D., Fokine ... The RV capsid protein is an essential structural component of virions as well as a key factor in virus-host interactions. Here ...
Therapies Targeting Capsid Proteins Show Promise for HIV-1
Therapies targeting capsid proteins show promise as candidates to combat drug resistance for human immunodeficiency virus type ... Investigators said that capsid proteins, because of their antiviral design, have indispensable roles in HIV-1 cytoplasmic ... By targeting the HIV-1 viral conical core, also known as the capsid or core, therapies could disrupt the process of assembly ... The core peptidic backbone of PF74 has also been applied to other capsid proteins targeting compounds, such as GS-6207, which ...
Figure 3 - Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway - Volume 8, Number 12...
Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway Joo-Sung Yang*1, Mathura P. ... In vivo West Nile virus capsid (Cp) expression induces apoptosis and inflammation in mice. TUNEL assay was performed on muscle ... Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway. ...
Residues on Adeno-associated Virus Capsid Lumen Dictate Interactions and Compatibility with the Assembly-Activating Protein
The viral cofactor assembly-activating protein (AAP) is required for maximum AAV production and has multiple roles in capsid ... The process of AAV capsid assembly remains poorly understood. ... Our results suggest a model for capsid assembly where surface ... These studies led to the identification of key residues on the lumenal capsid surface that are important for AAP-VP and for VP- ... Residues on Adeno-associated Virus Capsid Lumen Dictate Interactions and Compatibility with the Assembly-Activating Protein J ...
WikiGenes - VP3 - capsid protein VP3
The abundant nuclear enzyme PARP participates in the life cycle of simian virus 40 and is stimulated by minor capsid protein ... Polyomavirus VP1 phosphorylation: coexpression with the VP2 capsid protein modulates VP1 phosphorylation in Sf9 insect cells. ... Three putative capsid proteins VP1, VP2, and VP3 are encoded by alternative open reading frames [1]. ... Purified empty capsid-like particles, formed in nuclei of insect cells coinfected with all three baculoviruses, contained VP2 ...
SCOPe 2.08: Protein: Parvovirus (panleukopenia virus) capsid protein
... capsid from b.10.1.4: Animal virus proteins. *Protein Parvovirus (panleukopenia virus) capsid protein from b.121.5.2: ... Timeline for Protein Parvovirus (panleukopenia virus) capsid protein from b.121.5.2: Parvoviridae-like VP: *Protein Parvovirus ... Fold b.121: Nucleoplasmin-like/VP (viral coat and capsid proteins) [88632] (7 superfamilies). sandwich; 8 strands in 2 sheets; ... Lineage for Protein: Parvovirus (panleukopenia virus) capsid protein. *Root: SCOPe 2.08 *. Class b: All beta proteins [48724] ( ...
Crystal and solution structures reveal oligomerization of individual capsid homology domains of Drosophila Arc | PLOS ONE
Both dArc isoforms are related to the Ty3/gypsy retrotransposon capsid, consisting of N- and C-terminal lobes. Structures of ... The strong oligomerization properties of the isolated dArc lobe domains explain the ability of dArc to form capsids in the ... This domain-swapped structure resembles the dimeric protein C of flavivirus capsids, as well as the structure of histones ... Mammalian Arc forms virus-like capsid structures in a process requiring the N-terminal domain and contains two C-terminal lobes ...
HIV Capsid | NIH 3D Print Exchange
HIV needs the small molecule IP6 to build its capsid
During maturation, the viral protease cleaves Gag into single components and CA assembles into a mature capsid. The capsid ... In the mature capsid, R18 and K25 are thought to have a dual role. Both residues are important IP6 binders, and their mutation ... Here I show that infection and conical capsid formation of K25A can be rescued by compensating mutants without restoring the ... Both immature and mature HIV capsid lattices need to maintain a delicate balance of stability to allow efficient assembly ...
Recombinant Rotavirus A Intermediate capsid protein VP6 | CSB-EP321618RGK | Cusabio
Recombinant Rotavirus A Intermediate capsid protein VP6 from Cusabio. Cat Number: CSB-EP321618RGK. USA, UK & Europe ... Recombinant Rotavirus A Intermediate capsid protein VP6 , CSB-EP321618RGK. (No reviews yet) Write a Review Write a Review. ... Relevance: Intermediate capsid protein that self assbles to form an icosahedral capsid with a T=13 symmetry, which consists of ... Function: Intermediate capsid protein that self assembles to form an icosahedral capsid with a T=13 symmetry, which consists of ...
Size of typical structural capsid protein - Virus - BNID 112738
... analysis shows that the concept of a typical capsid protein is indeed viable - the typical capsid protein is prism-like, about ... Lošdorfer Božič A, Šiber A, Podgornik R. Statistical analysis of sizes and shapes of virus capsids and their resulting elastic ... Size of typical structural capsid protein. Range. mean diameter 5nm: thickness ~3nm nm ... P.12 3rd paragraph: Using the statistical analysis [investigators] have shown that capsid proteins of all viruses are ...
WO2021058002 CRYSTALLINE FORM OF CAPSID PROTEIN ASSEMBLY INHIBITOR CONTAINING N HETERO FIVE-MEMBERED RING, AND APPLICATION...
... said crystalline form in the preparation of a drug for preventing or treating diseases benefiting from the inhibition of capsid ... Disclosed by the present application is a crystalline form of a capsid protein assembly inhibitor containing an N hetero five- ... EN) Disclosed by the present application is a crystalline form of a capsid protein assembly inhibitor containing an N hetero ... EN) CRYSTALLINE FORM OF CAPSID PROTEIN ASSEMBLY INHIBITOR CONTAINING N HETERO FIVE-MEMBERED RING, AND APPLICATION THEREOF. ...
Non-covalent dendrimer- and polymer-based modifications of adenovirus capsids for enhanced transduction of cancer cells
LOINC 30137-4 - Epstein Barr virus capsid IgM Ab [Presence] in Serum --2nd specimen
LOINC Code 30137-4 Epstein Barr virus capsid IgM Ab [Presence] in Serum --2nd specimen ... EBV capsid IgM spec 2 Ql (S). Consumer Name Alpha. Epstein Barr virus (EBV) Capsid IgM antibody specimen 2, Blood. Basic ... Epstein Barr virus capsid Ab.IgM^2nd specimen. Property. PrThr. Time. Pt. System. Ser. Scale. Ord. Method. Additional Names. ... Epstein Barr virus capsid Ab.IgM^2nci örnek:. MevcEşik:. Zmlı:. Ser:. Srl:. Related Names. *ABS ...
Antios Therapeutics Announces Agreement with IRBM, INGM and OSR to Acquire Fourth-Generation HBV Capsid Assembly Modulators ...
Early in vivo data point to a differentiated mechanism of action which prevents accumulation of empty capsids, unlike most CAMs ... Antios Therapeutics Announces Agreement with IRBM, INGM and OSR to Acquire Fourth-Generation HBV Capsid Assembly Modulators ( ... "Unlike traditional CAMs, which target capsid assembly and disassembly, we believe these molecules may have an additional ... today they entered into an agreement for Antios to purchase the IP rights to a novel series of fourth-generation capsid ...
The Staphylococcal Cassette Chromosome mec type V from Staphylococcus aureus ST398 is packaged into bacteriophage capsids |...
Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the...
Identification of species-specific and cross-reactive epitopes in human polyomavirus capsids using monoclonal antibodies<...
Identification of species-specific and cross-reactive epitopes in human polyomavirus capsids using monoclonal antibodies. In: ... The human antibody response to polyomavirus capsid proteins is not well characterized. Recombinant BK virus (BKV), JC virus ( ... Dive into the research topics of Identification of species-specific and cross-reactive epitopes in human polyomavirus capsids ... N2 - The human antibody response to polyomavirus capsid proteins is not well characterized. Recombinant BK virus (BKV), JC ...
Detection of serum antibodies to hepatitis E virus in domestic pigs in Italy using a recombinant swine HEV capsid protein | BMC...
For specific HEV antibody detection in swine, we developed ELISA and Western blotting methods, using a truncated capsid (ORF2) ... Western blotting of the HEV rΔ111ORF2 capsid protein, stained with swine sera from this study (lanes 1 - 13). Positive sera ... In fact, the capsid proteins of human genotype 1 [30] and swine genotype 3 (this study) HEV shared 94.5% amino acid identity. ... Detection of serum antibodies to hepatitis E virus in domestic pigs in Italy using a recombinant swine HEV capsid protein. * ...
Identification of immunodominant and conformational epitopes in the capsid protein of hepatitis E virus by using monoclonal...
Antibody to the capsid (PORF2) protein of hepatitis E virus (HEV) is sufficient to confer immunity, but knowledge of B-cell ... Riddell, M. A. ; Li, F. ; Anderson, D. A. / Identification of immunodominant and conformational epitopes in the capsid protein ... N2 - Antibody to the capsid (PORF2) protein of hepatitis E virus (HEV) is sufficient to confer immunity, but knowledge of B- ... AB - Antibody to the capsid (PORF2) protein of hepatitis E virus (HEV) is sufficient to confer immunity, but knowledge of B- ...
Freedom and restraint: Themes in virus capsid assembly | [email protected]
Viruses assemble protective capsids from several copies of one or a few structural proteins. This is accomplished through a ... Dokland, T. (2000-08-15). Freedom and restraint: Themes in virus capsid assembly. Structure 8 (8) : R157-R162. [email protected] ...
Antigenic and phylogenetic relationships of outer capsid protein VP2, from multiple bluetongue virus serotypes - Nottingham...
Impact of Natural or Synthetic Singletons in the Capsid of Human Bocavirus 1 on Particle Infectivity and Immunoreactivity<...
Impact of Natural or Synthetic Singletons in the Capsid of Human Bocavirus 1 on Particle Infectivity and Immunoreactivity. ... Impact of Natural or Synthetic Singletons in the Capsid of Human Bocavirus 1 on Particle Infectivity and Immunoreactivity. / ... Impact of Natural or Synthetic Singletons in the Capsid of Human Bocavirus 1 on Particle Infectivity and Immunoreactivity. ... keywords = "bocavirus, BoV, capsid, mutations, HIGH-EFFICIENCY TRANSDUCTION, MINUTE VIRUS, SEROTYPE VECTORS, HUMAN PARVOVIRUS, ...
EN (en)
Poliovirus type 1 cDNA sequences coding for viral capsid polypeptide VP1 were inserted into the beta-lactamase sequence of ... Therefore, the C3 epitope responsible for virus neutralization is most probably located in this region of the capsid ... Localization of a poliovirus type 1 neutralization epitope in viral capsid polypeptide VP1. ... expressed in Escherichia coli a VP1-beta-lactamase fusion protein that reacted with antibodies raised against poliovirus capsid ...
Mouse Anti-Rotovirus capsid Protein [DS-MB-02239]
Assembly of bacteriophage 80α capsids in a Staphylococcus aureus expression system
We also find that SaPI1 proteins CpmA and CpmB promote the formation of small capsids upon co-expression with 80α capsid and ... Here, we show that co-expression of the 80α capsid and scaffolding proteins in S. aureus, but not in E. coli, leads to the ... The capsid and scaffolding proteins also undergo normal N-terminal processing upon expression in S. aureus, implicating a host ... Assembly of bacteriophage 80α capsids in a Staphylococcus aureus expression system Academic Article ...
Identification of a short, highly conserved, motif required for picornavirus capsid precursor processing at distal sites -...
For the aphthoviruses (e.g. FMDV) and cardioviruses, the capsid precursor, P1-2A, is cleaved by the 3C protease (3C pro ) to ... For the aphthoviruses (e.g. FMDV) and cardioviruses, the capsid precursor, P1-2A, is cleaved by the 3C protease (3C pro ) to ... For the aphthoviruses (e.g. FMDV) and cardioviruses, the capsid precursor, P1-2A, is cleaved by the 3C protease (3C pro ) to ... For enteroviruses, e.g. poliovirus, the capsid precursor is P1 alone, which is cleaved by the 3CD protease to generate just VP0 ...
Capsid Wings
Mint wings NFT can lead you to capsid zone,When you have blue chip NFT, like Azuki, BAYC, you can empower many rights to them ... Capsid wings project is an NFT derivative world, ... What is "Capsid Wings"? Through the wilderness of the Capsid ... These NFTs are powered by Capsid Protocol, an NFT derivative protocol that unlocks zillions of utilities for digital assets ...
Coxsackievirus B3: Primary structure of the 5′ non-coding and capsid protein-coding regions of the genome - Wikidata
Protein38
- From this density map we constructed a complete backbone trace of its major capsid protein, gene product 7 (gp7). (nature.com)
- Topologically linked protein rings in the bacteriophage HK97 capsid. (nature.com)
- The RV capsid protein is an essential structural component of virions as well as a key factor in virus-host interactions. (rcsb.org)
- Here we describe three crystal structures of the structural domain of the RV capsid protein. (rcsb.org)
- Combining the atomic structure of the RV capsid protein with the cryoelectron tomograms of RV particles established a low-resolution structure of the virion. (rcsb.org)
- The viral cofactor assembly-activating protein (AAP) is required for maximum AAV production and has multiple roles in capsid assembly, namely, trafficking of the structural proteins (VP) to the nuclear site of assembly, promoting the stability of VP against multiple degradation pathways, and facilitating stable interactions between VP monomers. (nih.gov)
- Studying interactions between the structural and nonstructural components of AAV enhances our fundamental knowledge of capsid assembly mechanisms and the protein-protein interactions required for productive assembly of the icosahedral capsid. (nih.gov)
- Polyomavirus VP1 phosphorylation: coexpression with the VP2 capsid protein modulates VP1 phosphorylation in Sf9 insect cells. (wikigenes.org)
- The viral capsid protein (CA) is essential for replication. (cam.ac.uk)
- Intermediate capsid protein that self assbles to form an icosahedral capsid with a T=13 symmetry, which consists of 230 trimers of VP6, with channels at each of its five-fold vertices. (joplink.net)
- A zinc ion controls assembly and stability of the major capsid protein of rotavirus.Erk I., Huet J.-C., Duarte M., Duquerroy S., Rey F.A., Cohen J., Lepault J.J. Virol. (joplink.net)
- Abstract: 'From the analysis of sizes of approximately 130 small icosahedral viruses [investigators] find that there is a typical structural capsid protein, having a mean diameter of 5 nm and a mean thickness of 3 nm, with more than two thirds of the analyzed capsid proteins having thicknesses between 2 nm and 4 nm. (harvard.edu)
- P.6 bottom paragraph: 'Taken together, [investigators'] analysis shows that the concept of a typical capsid protein is indeed viable - the typical capsid protein is prism-like, about 3 nm thick and has an average diameter of 5 nm. (harvard.edu)
- For specific HEV antibody detection in swine, we developed ELISA and Western blotting methods, using a truncated capsid (ORF2) protein lacking the first 111 amino acids of a swine HEV genotype 3 strain. (biomedcentral.com)
- In this study, we implemented a reliable ELISA test for detection of anti-HEV antibodies in swine sera, using a genotype 3 swine HEV capsid protein expressed by a recombinant baculovirus in insect cells as coating antigen. (biomedcentral.com)
- Antibody to the capsid (PORF2) protein of hepatitis E virus (HEV) is sufficient to confer immunity, but knowledge of B-cell epitopes in the intact capsid is limited. (monash.edu)
- A panel of murine monoclonal antibodies (MAbs) was generated following immunization with recombinant ORF2.1 protein, representing the C-terminal 267 amino acids (aa) of the 660-aa capsid protein. (monash.edu)
- These results suggest that sequences spanning aa 394 to 457 of the capsid protein participate in the formation of strongly immunodominant epitopes on the surface of HEV particles which may be important in immunity to HEV infection. (monash.edu)
- Resulting recombinant plasmid pSW119 expressed in Escherichia coli a VP1-beta-lactamase fusion protein that reacted with antibodies raised against poliovirus capsid polypeptide VP1 and with a monoclonal poliovirus type 1 neutralizing antibody, C3. (pasteur.fr)
- These small, non-enveloped viruses comprise a positive-stranded RNA genome (ca. 7-9 kb) enclosed within a protein shell composed of 60 copies of three or four different capsid proteins. (ku.dk)
- The sequences required for correct processing of the FMDV capsid protein precursor in mammalian cells were analyzed. (ku.dk)
- For the first time, the crystal structure of the JEV capsid protein at 1.98 Å is also reported and compared to the existing flavivirus capsid protein. (liverpool.ac.uk)
- JEV capsid shows helical secondary structure (α helixes 1-4) and protein folding similar to DENV and WNV capsid proteins. (liverpool.ac.uk)
- The HCV core protein and DENV capsid protein, extensively interact with LDs to regulate their replication and assembly. (frontiersin.org)
- Rationale: The capsid (C) protein of dengue virus functions primarily as a building block of the nucleocapsid. (who.int)
- Scientists at Thomas Jefferson University say they have pieced together the three-dimensional atomic structure of a doughnut-shaped protein that acts like a door or "portal" for the DNA to get in and out of the capsid, and have now discovered that this protein begins to transform its structure when it comes into contact with DNA. (medicilon.com)
- Their study (" Portal Protein Functions Akin to a DNA-Sensor That Couples Genome-Packaging to Icosahedral Capsid Maturation") is published in Nature Communications. (medicilon.com)
- We think that DNA binds to the immature portal protein and wraps around it like a python as it enters the viral capsid with the help of the motor protein. (medicilon.com)
- The capsid (CA) domain of the HIV-1 Gag protein is under strong pressure to conserve functional roles in viral assembly , maturation, uncoating, and nuclear import . (bvsalud.org)
- For long term storage, keep the Recombinant Human parvovirus B19 Non-capsid protein NS-1(NS1),partial frozen at -20 or -80 degrees Celsius. (hpv11.com)
- The Human parvovirus B19 (isolate AU) (HPV B19) parvovirus B19 Non-capsid protein NS-1(NS1),partial is shipped on ice packs. (hpv11.com)
- Detection of norovirus capsid protein in authentic standards and in stool extracts by matrix-assisted laser desorption ionization and nanospray mass spectrometry. (cdc.gov)
- The detection of norovirus capsid protein was explored using three common MS-based methods: scanning of intact proteins, peptide mass fingerprinting, and peptide sequencing. (cdc.gov)
- or =250 fmol of capsid protein in stool extracts. (cdc.gov)
- Nucleotide sequences encoding the major capsid protein VP1 were determined for each isolate. (cdc.gov)
- The different colors represent different regions of the organism's outer protein shell, or capsid. (cdc.gov)
- While it is a microscopic organism, it contains a nucleic acid core and an outer, protein casing known as the capsid. (jobs-freshers.com)
- ADENOVIRUS TYPE 40 HEXON PROTEIN Adenovirus type 40 hexon protein is a significant part of the adenovirus capsid. (mymouse.org)
Proteins18
- Therapies targeting capsid proteins show promise as candidates to combat drug resistance for human immunodeficiency virus type 1 (HIV-1) that antiretroviral therapies (ARTs) can cause, according to the results of a study published in Retrovirology . (pharmacytimes.com)
- Investigators reviewed different compounds that target 5 sites of the capsid proteins: apical binding site, CAP-1 binding site, central pore binding site FG binding site, and 2-fold binding site. (pharmacytimes.com)
- The drug "demonstrated sub-micromolar potency and serves as the backbone for some of the most promising current compounds, including PF74 analogues with increased potency, particularly against [capsid proteins] mutants that display resistance to PF74," according to the study. (pharmacytimes.com)
- The core peptidic backbone of PF74 has also been applied to other capsid proteins targeting compounds, such as GS-6207, which is an extremely potent therapy that is in phase 2/3 clinical trials. (pharmacytimes.com)
- However, the CAP-1 binding site demonstrated the potential of capsid proteins, as it was the first compound reported to target capsid proteins, investigators said. (pharmacytimes.com)
- Merck), which is a long-acting therapeutic that targets the capsid proteins and reverses the transcriptase, investigators said. (pharmacytimes.com)
- P.12 3rd paragraph: 'Using the statistical analysis [investigators] have shown that capsid proteins of all viruses are similarly sized, prism-like, about 3 nm thick, having an average diameter of 5 nm and an average molecular weight of mprot≈2.7×10^4 amu [atomic mass unit=Dalton]. (harvard.edu)
- The human antibody response to polyomavirus capsid proteins is not well characterized. (elsevier.com)
- Viruses assemble protective capsids from several copies of one or a few structural proteins. (nus.edu.sg)
- The capsid (CA) domain of retroviral Gag proteins posseses one subdomain, the major homology region (MHR) which is conserved among nearly all avian and mammalian retroviruses. (elsevier.com)
- The capsid proteins (CPs) of viruses in the order that infect unicellular organisms, such as algae, presumably possess certain characteristics that have changed little over the course of evolution, and thus these viruses may resemble the Picornavirales ancestor in some respects. (scilifelab.se)
- The sensitivity of real-time polymerase chain reaction (RT-PCR) is more than 1000 times greater than that of standard enzyme-linked immunosorbent assays and approximately 10-fold greater than reverse-transcription PCR in detection of norovirus capsid proteins in stool and food samples. (medscape.com)
- Tian P, Mandrell R. Detection of norovirus capsid proteins in faecal and food samples by a real time immuno-PCR method. (medscape.com)
- Capsids are composed of repeating units (capsomers or capsomeres) of CAPSID PROTEINS which when assembled together form either an icosahedral or helical shape. (bvsalud.org)
- During transformation of the procapsid into the mature VIRUS PARTICLE, some procapsid proteins, such as stabilizing viral scaffolding proteins, are ejected, and are therefore not part of the mature capsid structure. (bvsalud.org)
- The polyomaviruses have similar genetic makeup, including genes that encode viral capsid proteins VP1, 2, and 3 and large and small T region proteins. (web.app)
- To fully understand this interaction we will assess the role of other capsid proteins, fiber and penton base by using a range of chimeric adenoviral vectors. (ukri.org)
- Such a situation by calming down the the correct instructions on how to avoid frustration or embarrassment which can deter- someone who has longer duration of symp- toms) speeds up resolution of viral vaccine contamination have viruses) the capsid is surrounded by urfare proteins. (sdchirogroup.com)
Icosahedral2
- It consists of a double-stranded 172 Kb DNA genome, enclosed within an icosahedral capsid, surrounded by a phospholipid rich envelope Footnote 1 Footnote 3 . (canada.ca)
- It is a double-stranded DNA virus with a diameter of 150 to 200 nm, has an icosahedral capsid and a lipoprotein envelope 1 . (bvsalud.org)
Genome3
- The capsid protects the viral RNA genome which is reverse-transcribed into DNA, trafficked to the nucleus, and integrated into the host genome. (cam.ac.uk)
- Poliovirus is a type of anaerobe virus and consists of an RNA genome enclosed in a capsid. (cdc.gov)
- Viruses use a double-layered capsid and an envelope to protect their genome. (jobs-freshers.com)
Antibody1
- Mouse anti Rotavirus antibody, clone 0521 recognises the p41 capsid protien of Rotavirus from human, monkey and porcine isolates. (bio-rad-antibodies.com)
Viruses3
- The above-mentioned and other critical structural features provide new insights on three long-standing theories about Picornavirales: (i) the canyon hypothesis, (ii) the primordial VP2 domain swap, and (iii) the hypothesis that alga Picornavirales viruses could share characteristics with the Picornavirales ancestor.IMPORTANCE Identifying the acquired structural traits in virus capsids is important for elucidating what functions are essential among viruses that infect different hosts. (scilifelab.se)
- A comparison to capsid structures of the related invertebrate and vertebrate viruses identified a number of structural traits that have been functionally acquired or lost during the course of evolution. (scilifelab.se)
- But the picture of how that DNA is loaded into those capsids was blurry, especially for two of the most common types of DNA viruses on earth, bacterial viruses and human herpesvirus. (medicilon.com)
Full capsid1
- The SamuxMP mass photometer can quickly measure the empty/full capsid ratio during AAV downstream purification, making it ideal to use when optimizing and monitoring AAV purification processes. (refeyn.com)
Mature capsid3
- During maturation, the viral protease cleaves Gag into single components and CA assembles into a mature capsid. (cam.ac.uk)
- In the mature capsid, R18 and K25 are thought to have a dual role. (cam.ac.uk)
- Both residues are important IP6 binders, and their mutation interferes with mature capsid formation. (cam.ac.uk)
Target capsid1
- Unlike traditional CAMs, which target capsid assembly and disassembly, we believe these molecules may have an additional mechanism of action. (gabio.org)
Poliovirus capsid1
- These results can explain earlier observations that loss of the C-terminus (including the conserved motif) from the poliovirus capsid precursor conferred resistance to processing. (ku.dk)
Adeno-assoc1
- Skyline has established an advanced adeno-associated virus (AAV) based platform that seamlessly integrates cutting-edge capsid discovery, vector engineering, process development and GMP manufacturing, offering an unparalleled degree of quality and efficiency in the development of innovative gene therapies. (skytx.com)
Assembly8
- Here we report the 4.5-Å resolution structure of a 22-MDa macromolecular assembly, the capsid of the infectious epsilon15 (ε15) particle, by single-particle electron cryomicroscopy. (nature.com)
- Mutational studies based on this structure confirmed the role of amino acid residues in the capsid that function in the assembly of infectious virions. (rcsb.org)
- By targeting the HIV-1 viral conical core, also known as the capsid or core, therapies could disrupt the process of assembly and/or disassembly of the virus, resulting in the suppression of the virus. (pharmacytimes.com)
- The process of AAV capsid assembly remains poorly understood. (nih.gov)
- Our results suggest a model for capsid assembly where surface charge/neutrality dictates an interaction between AAPN and the lumenal VP surface to nucleate capsid assembly. (nih.gov)
- IMPORTANCE Efforts to engineer the AAV capsid to gain desirable properties for gene therapy (e.g., tropism, reduced immunogenicity, and higher potency) require that capsid modifications do not affect particle assembly. (nih.gov)
- Both immature and mature HIV capsid lattices need to maintain a delicate balance of stability to allow efficient assembly without compromising maturation or disassembly. (cam.ac.uk)
- Antios Therapeutics, Inc. ("Antios"), a clinical-stage biopharmaceutical company developing innovative therapies to treat and cure chronic hepatitis B virus (HBV), and IRBM, a drug discovery and early development research institute and global contract research organization, announced today they entered into an agreement for Antios to purchase the IP rights to a novel series of fourth-generation capsid assembly modulators (CAMs) being developed for the treatment of HBV. (gabio.org)
Antigen1
- The presence or absence of EBV viral capsid antigen (VCA) IgG, VCA IgM and EBV nuclear antigen (EBNA) IgG antibodies was recorded. (who.int)
Protects the viral1
- The capsid protects the viral nucleic acids from nucleases, which are essential to their replication. (jobs-freshers.com)
Residues2
- These studies led to the identification of key residues on the lumenal capsid surface that are important for AAP-VP and for VP-VP interactions. (nih.gov)
- Four different functional domains were analyzed: 1) neutralizing antigenic (NAg) sites, 2) residues binding the poliovirus receptor (PVR), 3) VP1 residues 1-32, and 4) the capsid structural core. (cdc.gov)
Particle2
- This capsid constitutes the middle concentric layer of the viral mature particle. (joplink.net)
- We have shown that the portal is much more like a sensor that essentially helps measure out an appropriate length of DNA for each capsid particle, ensuring faithful production of new viral particles. (medicilon.com)
Polypeptide3
- The polypeptide fold of the RV capsid protomer has not been observed previously. (rcsb.org)
- Poliovirus type 1 cDNA sequences coding for viral capsid polypeptide VP1 were inserted into the beta-lactamase sequence of Escherichia coli plasmid pBR322. (pasteur.fr)
- Therefore, the C3 epitope responsible for virus neutralization is most probably located in this region of the capsid polypeptide. (pasteur.fr)
Interactions1
- Capsid model and interactions of gp7. (nature.com)
Inhibitors2
- This helped expand the field of HIV-1 capsid inhibitors for each of the 5 binding sites discussed. (pharmacytimes.com)
- Lenacapavir is in a class of medications called HIV capsid inhibitors. (medlineplus.gov)
Amino acid1
- We followed the dynamics of capsid amino acid replacement among 403 Nigerian outbreak isolates of type 2 circulating vaccine-derived poliovirus (cVDPV2) from 2005 through 2011. (cdc.gov)
Replication1
- The innermost VP2 capsid and the intermediate VP6 capsid rain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. (joplink.net)
Virus7
- In vivo West Nile virus capsid (Cp) expression induces apoptosis and inflammation in mice. (cdc.gov)
- Lošdorfer Božič A, Šiber A, Podgornik R. Statistical analysis of sizes and shapes of virus capsids and their resulting elastic properties. (harvard.edu)
- Capsid Structure of a Marine Algal Virus of the Order Picornavirales. (scilifelab.se)
- To create swarms of new viral particles, a virus hijacks a cell into producing masses of self-assembling "cages," or capsids, that are then loaded with the genetic blueprint for the next infection. (medicilon.com)
- En aquesta tesi hem presentat estratègies per millorar la disseminació intratumoral basades en l'augment de l'alliberament viral i en la millora de l'extravasació i penetració del virus al parènquima tumoral, augmentant en els dos casos l'eficàcia antitumoral. (ub.edu)
- Aquest virus també presenta alliberament viral, citotoxicitat i eficàcia antitumoral incrementades en un model de carcinoma de pulmó humà. (ub.edu)
- En el model de hàmster siri, un model immunocompetent i semipermissiu a la replicació de l'adenovirus, aquest virus presentava eficàcia antitumoral només al combinar-lo amb gemcitabina. (ub.edu)
Vector1
- Additionally, library-based approaches that simultaneously examine a large number of capsid variants would benefit from a universally functional AAP, which could hedge against overlooking variants with potentially valuable phenotypes that were lost during vector library production due to incompatibility with the cognate AAP. (nih.gov)
Conical1
- Here I show that infection and conical capsid formation of K25A can be rescued by compensating mutants without restoring the charged K25 ring. (cam.ac.uk)
Antivirals1
- Rotten to the core: antivirals targeting the HIV-1 capsid core. (pharmacytimes.com)
Therapeutic1
- Evaluate/Determine/Explore the optimal media for increased titers and VG/capsid ratios for therapeutic development. (insights.bio)
Isolates1
- This product recognizes the p41 capsid protien of Rotavirus from human, monkey and porcine isolates. (raybiotech.com)
Serotypes1
- Designed to improve growth and production of AAV and AAV2 serotypes, the HSMP optimizes HEK293 media allowing for increased titers and improved VG/Capsid ratios. (insights.bio)
Atomic1
- This article describes the first atomic capsid structure of an alga Marnavirus, CtenRNAV-II. (scilifelab.se)