The minute vessels that connect the arterioles and venules.
A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992)
A phenomenon in which the surface of a liquid where it contacts a solid is elevated or depressed, because of the relative attraction of the molecules of the liquid for each other and for those of the solid. (from McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The property of blood capillary ENDOTHELIUM that allows for the selective exchange of substances between the blood and surrounding tissues and through membranous barriers such as the BLOOD-AIR BARRIER; BLOOD-AQUEOUS BARRIER; BLOOD-BRAIN BARRIER; BLOOD-NERVE BARRIER; BLOOD-RETINAL BARRIER; and BLOOD-TESTIS BARRIER. Small lipid-soluble molecules such as carbon dioxide and oxygen move freely by diffusion. Water and water-soluble molecules cannot pass through the endothelial walls and are dependent on microscopic pores. These pores show narrow areas (TIGHT JUNCTIONS) which may limit large molecule movement.
A dull red, firm, dome-shaped hemangioma, sharply demarcated from surrounding skin, usually located on the head and neck, which grows rapidly and generally undergoes regression and involution without scarring. It is caused by proliferation of immature capillary vessels in active stroma, and is usually present at birth or occurs within the first two or three months of life. (Dorland, 27th ed)
The vascular resistance to the flow of BLOOD through the CAPILLARIES portions of the peripheral vascular bed.
A separation technique which combines LIQUID CHROMATOGRAPHY and CAPILLARY ELECTROPHORESIS.
A condition characterized by recurring episodes of fluid leaking from capillaries into extra-vascular compartments causing hematocrit to rise precipitously. If not treated, generalized vascular leak can lead to generalized EDEMA; SHOCK; cardiovascular collapse; and MULTIPLE ORGAN FAILURE.
The susceptibility of CAPILLARIES, under conditions of increased stress, to leakage.
The circulation of the BLOOD through the MICROVASCULAR NETWORK.
The blood vessels which supply and drain the RETINA.
The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.
Hollow cylindrical objects with an internal diameter that is small enough to fill by and hold liquids inside by CAPILLARY ACTION.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
A tissue preparation technique that involves the injecting of plastic (acrylates) into blood vessels or other hollow viscera and treating the tissue with a caustic substance. This results in a negative copy or a solid replica of the enclosed space of the tissue that is ready for viewing under a scanning electron microscope.
A cluster of convoluted capillaries beginning at each nephric tubule in the kidney and held together by connective tissue.
The thin, horny plates that cover the dorsal surfaces of the distal phalanges of the fingers and toes of primates.
The noninvasive microscopic examination of the microcirculation, commonly done in the nailbed or conjunctiva. In addition to the capillaries themselves, observations can be made of passing blood cells or intravenously injected substances. This is not the same as endoscopic examination of blood vessels (ANGIOSCOPY).
Unique slender cells with multiple processes extending along the capillary vessel axis and encircling the vascular wall, also called mural cells. Pericytes are imbedded in the BASEMENT MEMBRANE shared with the ENDOTHELIAL CELLS of the vessel. Pericytes are important in maintaining vessel integrity, angiogenesis, and vascular remodeling.
The circulation of the BLOOD through the LUNGS.
The blood pressure as recorded after wedging a CATHETER in a small PULMONARY ARTERY; believed to reflect the PRESSURE in the pulmonary CAPILLARIES.
The flow of BLOOD through or around an organ or region of the body.
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
A darkly stained mat-like EXTRACELLULAR MATRIX (ECM) that separates cell layers, such as EPITHELIUM from ENDOTHELIUM or a layer of CONNECTIVE TISSUE. The ECM layer that supports an overlying EPITHELIUM or ENDOTHELIUM is called basal lamina. Basement membrane (BM) can be formed by the fusion of either two adjacent basal laminae or a basal lamina with an adjacent reticular lamina of connective tissue. BM, composed mainly of TYPE IV COLLAGEN; glycoprotein LAMININ; and PROTEOGLYCAN, provides barriers as well as channels between interacting cell layers.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
The smallest divisions of the arteries located between the muscular arteries and the capillaries.
The minute vessels that collect blood from the capillary plexuses and join together to form veins.
The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.
A highly miniaturized version of ELECTROPHORESIS performed in a microfluidic device.
Microscopy in which television cameras are used to brighten magnified images that are otherwise too dark to be seen with the naked eye. It is used frequently in TELEPATHOLOGY.
Specialized non-fenestrated tightly-joined ENDOTHELIAL CELLS with TIGHT JUNCTIONS that form a transport barrier for certain substances between the cerebral capillaries and the BRAIN tissue.
A hypoperfusion of the BLOOD through an organ or tissue caused by a PATHOLOGIC CONSTRICTION or obstruction of its BLOOD VESSELS, or an absence of BLOOD CIRCULATION.
The carbohydrate-rich zone on the cell surface. This zone can be visualized by a variety of stains as well as by its affinity for lectins. Although most of the carbohydrate is attached to intrinsic plasma membrane molecules, the glycocalyx usually also contains both glycoproteins and proteoglycans that have been secreted into the extracellular space and then adsorbed onto the cell surface. (Alberts et al., Molecular Biology of the Cell, 3d ed, p502)
A value equal to the total volume flow divided by the cross-sectional area of the vascular bed.
A subtype of striated muscle, attached by TENDONS to the SKELETON. Skeletal muscles are innervated and their movement can be consciously controlled. They are also called voluntary muscles.
Elements of limited time intervals, contributing to particular results or situations.
A disorder of the skin, the oral mucosa, and the gingiva, that usually presents as a solitary polypoid capillary hemangioma often resulting from trauma. It is manifested as an inflammatory response with similar characteristics to those of a granuloma.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
The movement and the forces involved in the movement of the blood through the CARDIOVASCULAR SYSTEM.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.
The taking of a blood sample to determine its character as a whole, to identify levels of its component cells, chemicals, gases, or other constituents, to perform pathological examination, etc.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
The vessels carrying blood away from the capillary beds.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
The volume of packed RED BLOOD CELLS in a blood specimen. The volume is measured by centrifugation in a tube with graduated markings, or with automated blood cell counters. It is an indicator of erythrocyte status in disease. For example, ANEMIA shows a low value; POLYCYTHEMIA, a high value.
Disease of the RETINA as a complication of DIABETES MELLITUS. It is characterized by the progressive microvascular complications, such as ANEURYSM, interretinal EDEMA, and intraocular PATHOLOGIC NEOVASCULARIZATION.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Any of the tubular vessels conveying the blood (arteries, arterioles, capillaries, venules, and veins).
Treatment process involving the injection of fluid into an organ or tissue.
The barrier between capillary blood and alveolar air comprising the alveolar EPITHELIUM and capillary ENDOTHELIUM with their adherent BASEMENT MEMBRANE and EPITHELIAL CELL cytoplasm. PULMONARY GAS EXCHANGE occurs across this membrane.
Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
The finer blood vessels of the vasculature that are generally less than 100 microns in internal diameter.
A layer of the peritoneum which attaches the abdominal viscera to the ABDOMINAL WALL and conveys their blood vessels and nerves.
PRESSURE of the BLOOD on the ARTERIES and other BLOOD VESSELS.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The amount of a gas taken up, by the pulmonary capillary blood from the alveolar gas, per minute per unit of average pressure of the gradient of the gas across the BLOOD-AIR BARRIER.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Excessive accumulation of extravascular fluid in the lung, an indication of a serious underlying disease or disorder. Pulmonary edema prevents efficient PULMONARY GAS EXCHANGE in the PULMONARY ALVEOLI, and can be life-threatening.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
Small polyhedral outpouchings along the walls of the alveolar sacs, alveolar ducts and terminal bronchioles through the walls of which gas exchange between alveolar air and pulmonary capillary blood takes place.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
Inflammation of the renal glomeruli (KIDNEY GLOMERULUS) that can be classified by the type of glomerular injuries including antibody deposition, complement activation, cellular proliferation, and glomerulosclerosis. These structural and functional abnormalities usually lead to HEMATURIA; PROTEINURIA; HYPERTENSION; and RENAL INSUFFICIENCY.
An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.
The force that opposes the flow of BLOOD through a vascular bed. It is equal to the difference in BLOOD PRESSURE across the vascular bed divided by the CARDIAC OUTPUT.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
A family of angiogenic proteins that are closely-related to VASCULAR ENDOTHELIAL GROWTH FACTOR A. They play an important role in the growth and differentiation of vascular as well as lymphatic endothelial cells.
These growth factors are soluble mitogens secreted by a variety of organs. The factors are a mixture of two single chain polypeptides which have affinity to heparin. Their molecular weight are organ and species dependent. They have mitogenic and chemotactic effects and can stimulate endothelial cells to grow and synthesize DNA. The factors are related to both the basic and acidic FIBROBLAST GROWTH FACTORS but have different amino acid sequences.
A system of organs and tissues that process and transport immune cells and LYMPH.
Inorganic or organic salts and esters of boric acid.
Agents that induce or stimulate PHYSIOLOGIC ANGIOGENESIS or PATHOLOGIC ANGIOGENESIS.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
The circulation of blood through the BLOOD VESSELS of the BRAIN.
The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.
Volume of circulating BLOOD. It is the sum of the PLASMA VOLUME and ERYTHROCYTE VOLUME.
The rate at which oxygen is used by a tissue; microliters of oxygen STPD used per milligram of tissue per hour; the rate at which oxygen enters the blood from alveolar gas, equal in the steady state to the consumption of oxygen by tissue metabolism throughout the body. (Stedman, 25th ed, p346)
Cell adhesion molecules present on virtually all monocytes, platelets, and granulocytes. CD31 is highly expressed on endothelial cells and concentrated at the junctions between them.
A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An area approximately 1.5 millimeters in diameter within the macula lutea where the retina thins out greatly because of the oblique shifting of all layers except the pigment epithelium layer. It includes the sloping walls of the fovea (clivus) and contains a few rods in its periphery. In its center (foveola) are the cones most adapted to yield high visual acuity, each cone being connected to only one ganglion cell. (Cline et al., Dictionary of Visual Science, 4th ed)
Theoretical representations that simulate the behavior or activity of the cardiovascular system, processes, or phenomena; includes the use of mathematical equations, computers and other electronic equipment.
Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.
Visualization of a vascular system after intravenous injection of a fluorescein solution. The images may be photographed or televised. It is used especially in studying the retinal and uveal vasculature.
A type of stress exerted uniformly in all directions. Its measure is the force exerted per unit area. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.
The veins and arteries of the HEART.
The large fragment formed when COMPLEMENT C4 is cleaved by COMPLEMENT C1S. The membrane-bound C4b binds COMPLEMENT C2A, a SERINE PROTEASE, to form C4b2a (CLASSICAL PATHWAY C3 CONVERTASE) and subsequent C4b2a3b (CLASSICAL PATHWAY C5 CONVERTASE).
Normal human serum albumin mildly iodinated with radioactive iodine (131-I) which has a half-life of 8 days, and emits beta and gamma rays. It is used as a diagnostic aid in blood volume determination. (from Merck Index, 11th ed)
A vessel that directly interconnects an artery and a vein, and that acts as a shunt to bypass the capillary bed. Not to be confused with surgical anastomosis, nor with arteriovenous fistula.
"Chapter 2 Evolution and development of isoelectric focusing". In Garfin, David; Ahuja, Satinder (eds.). Handbook of isoelectric ... His work on capillary zone electrophoresis (CZE) supports the identification of DNA point mutations. He has been involved in ... Righetti, P. G.; Drysdale, J. W. (1976). Isoelectric focusing. New York: American Elsevier Pub. Co. 2014, AES Award, American ... They developed techniques for isoelectric focusing (IEF) using soluble carrier ampholytes and polyacrylamide gels. In 1971, ...
New techniques like "capillary isoelectric focusing immunoassay" are able to detect IgG OCBs in more than 95% of multiple ... "Detection of intrathecal immunoglobulin G synthesis by capillary isoelectric focusing immunoassay in oligoclonal band negative ... the combination of isoelectric focusing/silver staining. The latter is more sensitive. For the analysis of cerebrospinal fluid ... of virus-specific oligoclonal bands in subacute sclerosing panencephalitis by immunofixation after isoelectric focusing and ...
Isoelectric focusing is the separation of proteins, organelles, and cells with different isoelectric points. Isoelectric ... Microfluidic sample separation can be achieved by capillary electrophoresis or continuous-flow separation. In capillary ... Examples of continuous-flow separation techniques include continuous-flow electrophoresis, isoelectric focusing, continuous- ... Capillary electrophoresis thus became a focus for chemical and DNA separation. Thirdly, DARPA of the US Department of Defense ...
Electroblotting Gel electrophoresis Gel electrophoresis of nucleic acids Immunoelectrophoresis Isoelectric focusing ... Affinity electrophoresis Electrophoretic deposition Capillary electrophoresis Dielectrophoresis ...
MATRICE OF Spirulina platensis Determination of the pI of Human Rhinovirus Serotype 2 by Capillary Isoelectric Focusing The ... binding strength of Cd(II) to C-phycocyanin from Spirulina platensis Capillary electrophoresis of Cr(VI) reducer Arthrobacter ...
After separation, an additional separation method may then be used, such as isoelectric focusing or SDS-PAGE. The gel will then ... introduction of capillary electrophoresis 2004 - introduction of a standardized time of polymerization of acrylamide gels ... 2D gel analysis software History of electrophoresis Electrophoretic mobility shift assay Gel extraction Isoelectric focusing ... isoelectric focusing then SDS gel electrophoresis 1977 - sequencing gels 1983 - pulsed field gel electrophoresis enables ...
... it was used to couple MS with various separation techniques including capillary electrophoresis and gel isoelectric focusing, ... ESTASI method can be applied to a wide range of geometries, e.g. samples in capillary, in a disposable pipette tip, in a ... The current developed applications of ESTASI mainly include: Fractions of proteins or peptides from capillary electrophoresis ... and a square wave HV is applied between the electrode and the mass spectrometer inlet capillary. Sample ionization occurs and ...
History of electrophoresis Isoelectric focusing Isotachophoresis Native gel electrophoresis Northern blotting Protein ... Agarose gel electrophoresis Capillary electrophoresis DNA electrophoresis Eastern blotting Electroblotting Fast parallel ... as each protein has an isoelectric point and molecular weight particular to its primary structure. This is known as native PAGE ...
Apparatus for protein separation using capillary isoelectric focusing-hollow fiber flow field flow fractionation and method ...
"Multijunction Capillary Isoelectric Focusing Device Combined with Online Membrane-Assisted Buffer Exchanger Enables Isoelectric ... Isoelectric focusing can resolve proteins that differ in pI value by as little as 0.01. Isoelectric focusing is the first step ... Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in ... According to some opinions, living eukaryotic cells perform isoelectric focusing of proteins in their interior to overcome a ...
... capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar electrokinetic chromatography (MEKC) belong also ... Other capillaries including Teflon capillaries also exhibit electroosmotic flow. The EOF of these capillaries is probably the ... Sample is introduced into the capillary via capillary action, pressure, siphoning, or electrokinetically, and the capillary is ... Detection occurs via fluorescence through a window etched in the capillary. Both single-capillary and capillary-array ...
... including capillary isotachophoresis and capillary isoelectric focusing. There are two common techniques to load the sample ... This technique uses a stainless steel tee to mix separation electrolyte from CE capillary with make up liquid. The CE capillary ... In the first CE-MS interface a stainless steel capillary sheath around the separation capillary terminus was used instead of ... The terminus of separation capillary was protruded from the tapered sheath-flow capillary. Because of thin wall of the ...
... isoelectric focusing MeSH E05.196.454.807 - ultrafiltration MeSH E05.196.454.807.300 - hemofiltration MeSH E05.196.454.807. ... capillary MeSH E05.196.401.190.500 - electrophoresis, microchip MeSH E05.196.401.200 - electrophoresis, cellulose acetate MeSH ... micellar electrokinetic capillary MeSH E05.196.181.750 - chromatography, supercritical fluid MeSH E05.196.309.555 - neutron ...
Wang S, Chen S, Wang J, Xu P, Luo Y, Nie Z, Du W (September 2014). "Interface solution isoelectric focusing with in situ MALDI- ... In a T-junction, droplet size and formation rate are determined by the flow rate ratio and capillary number. The capillary ... and 2-dimensional capillary flow chromatography (capillary LC), have been integrated into the field of droplet-based ... Flow focusing can also be an active method with the constraint point being adjustable using pneumatic side chambers controlled ...
In the first dimension, the proteins are separated by isoelectric focusing, which resolves proteins on the basis of charge. In ... Some newer methods for the separation and identification of proteins include the use of monolithic capillary columns, high ... Kozlowski, LP (26 October 2016). "Proteome-pI: proteome isoelectric point database". Nucleic Acids Research. 45 (D1): D1112- ... temperature chromatography and capillary electrochromatography. Western blotting can be used in order to quantify the abundance ...
Isoelectric focusing. *Isotachophoresis. *Native gel electrophoresis. *Northern blotting. *Protein electrophoresis. *Southern ... Capillary electrochromatography. *Capillary electrophoresis. *Dielectrophoresis. *Difference gel electrophoresis. * ... as each protein has an isoelectric point and molecular weight particular to its primary structure. This is known as native PAGE ...
... isoelectric focusing or (interval) zone electrophoresis. At the end of the separation cell, the separated sample is split up at ... FFE is an analogous technique to capillary electrophoresis, with a comparable resolution, that can used for scientific ... Analytes in the laminar flow can be separated by charge density and/or isoelectric point. Because of its highly versatile ... and mode of operation High reproducibility of individual runs Separation in several minutes Protocols for Isoelectric Focusing ...
Capillary electrochromatography. *Capillary electrophoresis. *Dielectrophoresis. *Difference gel electrophoresis. * ...
Capillary electrophoresis[edit]. In capillary electrophoresis, there is no solid matrix. Proteins are separated primarily by ... The sample is injected into a capillary with a negative surface charge. A high current is applied, and negatively charged ... Depending on whether an agarose or capillary method is used, interferences vary. Immunoglobulins consist of heavy chains (IgA, ... or into liquid in a capillary tube, and exposed to an electric current to separate the serum protein components into five major ...
The migration behavior of ions from the hydrogels into the capillary and the formation of a pH gradient in isoelectric focusing ... focusing using short capillaries in place of the acid and base solutions used in conventional microfluidic isoelectric focusing ... which often decreases the reproducibility in capillary isoelectric focusing. ... The prepared reagent-release hydrogels showed some advantages for isoelectric focusing, e.g., high durability for at least five ...
... device containing a reagent-release capillary (RRC) array and poly(dimethylsiloxane) (PDMS) platform, which allows rapid ( ... This report describes the fabrication and characterization of a simple and disposable capillary isoelectric focusing (cIEF) ... capillary isoelectric focusing. (. cIEF. ) device containing a reagent-release capillary (RRC) array and poly(dimethylsiloxane ... Reagent-release capillary array-. isoelectric focusing. device as a rapid screening device for IEF. condition optimization M. ...
... data obtained by capillary isoelectric focusing could be confirmed with gel isoelectric focusing. The two capillary ... data obtained by capillary isoelectric focusing could be confirmed with gel isoelectric focusing. The two capillary ... a double peak in capillary isoelectric focusing only and a double peak in capillary zone electrophoresis only. For all samples ... a double peak in capillary isoelectric focusing only and a double peak in capillary zone electrophoresis only. For all samples ...
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Dynamic Kinetic Capillary Isoelectric Focusing: A Powerful Tool for Studying Protein−DNA Interactions ...
Capillary isoelectric focusing (cIEF) was online coupled to a Q-TOF MS by a flow-through microvial interface for the analysis ... Analysis of four therapeutic monoclonal antibodies by online capillary isoelectric focusing directly coupled to quadrupole time ... Analysis of four therapeutic monoclonal antibodies by online capillary isoelectric focusing directly coupled to quadrupole time ... and catholyte segments sequentially injected into a neutrally coated capillary was used for the charge heterogeneity separation ...
A microfluidic system continues to be designed that integrates both imaged capillary isoelectric focusing (iCIEF) separations ... A microfluidic system continues to be designed that integrates both imaged capillary isoelectric focusing (iCIEF) separations ... MS capillary potential was arranged between ?3750 to ?4250?V with drying gas collection at 6 L/min and drying heat set at 220C ... The focusing stage was initiated through the use of an optimistic 1500?V in the Anode (Anolyte) power while the power in ...
Clinical application of capillary isoelectric focusing on fused silica capillary for determination of hemoglobin variants. ... Clinical application of capillary isoelectric focusing on fused silica capillary for determination of hemoglobin variants. / ... Clinical application of capillary isoelectric focusing on fused silica capillary for determination of hemoglobin variants. ... T1 - Clinical application of capillary isoelectric focusing on fused silica capillary for determination of hemoglobin variants ...
"Chapter 2 Evolution and development of isoelectric focusing". In Garfin, David; Ahuja, Satinder (eds.). Handbook of isoelectric ... His work on capillary zone electrophoresis (CZE) supports the identification of DNA point mutations. He has been involved in ... Righetti, P. G.; Drysdale, J. W. (1976). Isoelectric focusing. New York: American Elsevier Pub. Co. 2014, AES Award, American ... They developed techniques for isoelectric focusing (IEF) using soluble carrier ampholytes and polyacrylamide gels. In 1971, ...
This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are ... coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device ... A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously ... excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. ...
Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have ... capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITP), capillary gel electrophoresis (CGE), and micellar ... In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis ( ... Glycoprotein Analysis by Capillary Zone Electrophoresis-Electrospray Mass Spectrometry Kevin P. Bateman, John F. Kelly, Pierre ...
For the separation of intact proteins, 1DGE or 2DGE, capillary isoelectric focusing, ion exchange chromatography, and HPLC (C4 ... capillary isoelectric focusing, HPLC, or ion exchange chromatography. 1DGE separations (with the exception of modification by ... capillary isoelectric focusing; MIDA, mass isotopomer distribution analysis; IMAC, immobilized metal affinity chromatography. ... leucine into Escherichia coli proteins and rapid separation by capillary isoelectric focusing. The masses of labeled and ...
Capillary Isoelectric Focusing (cIEF). *Congestive Heart Failure (CHF) fluid monitoring. *DNA/molecular sequencing systems ...
Capillary. * High performance liquid chromatography (HPLC). *Other advanced techniques *Isoelectric focusing. *Globin chain ... HbA has isoelectric point of 6.8 *Negative charge in alkaline buffers ⇒ migrates toward anode (+) ...
7.5.2 Isoelectric Focusing. 7.5.3 Capillary Electrophoresis. 7.6 Western Blotting (Immunoblotting) 7.7 Dialysis and ... Table 23 Global Isoelectric Focusing Market Size, By Country, 2013 2020 ($Million). Table 24 Global Capillary Electrophoresis ... In order to maintain their market shares, leading players are continuously focusing on developing innovative protein ...
The metabolic profiles of brain biopsies obtained at surgery were recorded using capillary gas chromatography (GC). About 160 ... Isoelectric Focusing * Neoplasm Proteins / analysis* * Pattern Recognition, Automated * Pituitary Neoplasms / metabolism ... Classification of human cancer cells by means of capillary gas chromatography and pattern recognition analysis J Chromatogr. ... The combined use of the latter technique and capillary GC-mass spectrometry and pattern recognition analyses gives the ...
Conventional Isoelectric Focusing and Immobilized pH Gradients; P.G. Righetti, C. Tonani. Solute Band Spreading in Capillary ...
Hemoglobin isoelectric focusing (Hb IEF). *Hemoglobin by high performance liquid chromatography (HPLC) ... Hemoglobin by capillary zone electrophoresis. *Hemoglobin by mass spectrometry. These methods evaluate the different types of ...
... isoelectric-focusing gel electrophoresis (IEF), and capillary equivalents such as capillary isoelectric focusing (CIEF) and ... Imaged Capillary Isoelectric Focusing for Charge-Variant Analysis of Biopharmaceuticals. by Chantal Felten, PhD, Oscar Salas- ...
Capillary isoelectric focusing allows the fast and simple determination of isoelectric points of relevant antibiotics, their ... The separation conditions for the capillary isoelectric focusing of selected ampholytic antibiotics with known isoelectric ... For this reason, capillary isoelectric focusing seems to be appropriate as a simple and reliable way for establishing them. ... The capillary isoelectric focusing is a suitable technique for the separation and the detection of bacteria, and amphoteric ...
Abbreviations: cIEF, capillary isoelectric focusing; pI, isoelectric point; eCAP, amine capillary with laser burned window; UV ... Capillary isoelectric focusing (cIEF) of adalimumab samples was performed on a Beckman PA800 Plus instrument. Samples ... capillary (50 μm×20 cm), and focusing was performed with 30 kV for 30 minutes at 25 psi and 20°C. Electropherogram was ... capillary. After pre-focusing at 25 kV for 15 minutes, samples were focused at 30 kV for 30 minutes. Separation was monitored ...
Isoelectric Focusing (IEF) - An electrophoretic method which separates proteins by their pI. They move through a pH gradient ... Capillary Electrophoresis - Used as a complement to HPLC, particularly for peptide mapping. This technique is faster and will ... An analyte is placed on an electrophoretic support, then separated by charge (isoelectric focusing) or by molecular weight (SDS ... medium in an electric field until they are located at their isoelectric point where they carry no net charge. Prior to reaching ...
Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method, A ... Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method. Narendra ...
Capillary gel electrophoresis. CI. Chemical ionization. CIEF. Capillary isoelectric focusing. CITP. Capillary isotachophoresis ... Whatley H (2001) Basic principles and modes of capillary electrophoresis. In: Clinical and forensic applications of capillary ... Chemo-and enantio-selective method for the analysis of amino acids by capillary electrophoresis with in-capillary ... Song Y, Feng Y, LeBlanc MH, Zhao S, Liu YM (2006) Assay of trace d-amino acids in neural tissue samples by capillary liquid ...
... capillary electrophoresis of compounds of biological interest; isoelectric focusing; mass spectrometry of biomolecules; liquid- ... capillary electrophoresis of compounds of biological interest; isoelectric focusing; mass spectrometry of biomolecules; liquid- ...
Separation of Recombinant Therapeutic Proteins Using Capillary Gel Electrophoresis and Capillary Isoelectric Focusing. ...
Dynamic capillary coatings with zwitterionic surfactants for capillary isoelectric focusing. The Analyst 128: 566-570. ...
Capillary Isoelectric Focusing Immobilized Enzyme Microreactor Integrated Platform Nanoreversed-phase Liquid Chromatography ... Integrated platform of capillary isoelectric focusing, trypsin immobilized enzyme microreactor and nanoreversed-phase liquid ... Integrated platform of capillary isoelectric focusing, trypsin immobilized enzyme microreactor and nanoreversed-phase liquid ... Wang, Tingting,et al."Integrated platform of capillary isoelectric focusing, trypsin immobilized enzyme microreactor and ...
Capillary Isoelectric Focusing Market. 4.1.1.2.5. Micellar Electrokinetic Capillary Chromatography. 4.1.1.2.6. Capillary ... Capillary Electrophoresis Market. 4.1.1.2.1.1.1. Capillary Gel Electrophoresis Market. 4.1.1.2.2. Capillary Zone ... 4.1.1.2.3. Capillary Electrochromatography (Cec) Market. 4.1.1.2.4. ... Electrophoresis Market by Product (Gel Electrophoresis , Capillary Electrophoresis, Reagents, Geldoc, Imaging), Application ( ...
New techniques like "capillary isoelectric focusing immunoassay" are able to detect IgG OCBs in more than 95% of multiple ... "Detection of intrathecal immunoglobulin G synthesis by capillary isoelectric focusing immunoassay in oligoclonal band negative ... the combination of isoelectric focusing/silver staining. The latter is more sensitive. For the analysis of cerebrospinal fluid ... of virus-specific oligoclonal bands in subacute sclerosing panencephalitis by immunofixation after isoelectric focusing and ...
  • This report describes the fabrication and characterization of a simple and disposable capillary isoelectric focusing ( cIEF ) device containing a reagent-release capillary (RRC) array and poly(dimethylsiloxane) (PDMS) platform, which allows rapid (within 10 min) screening of cIEF conditions by introducing a sample solution into plural RRCs by capillary action followed by electric field application. (rsc.org)
  • Capillary isoelectric focusing (cIEF) was online coupled to a Q-TOF MS by a flow-through microvial interface for the analysis of therapeutic mAb. (orphiz.be)
  • A sandwich cIEF-MS configuration composed of anolyte, sample, and catholyte segments sequentially injected into a neutrally coated capillary was used for the charge heterogeneity separation of four mAbs. (orphiz.be)
  • Different CE separation formats, including capillary zone electrophoresis (CZE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITP), capillary gel electrophoresis (CGE), and micellar electrokinetic chromatography (MEKC), are presented for different types of applications, such as the analysis of glycoforms, the separation of enantiomers, and the resolution of oligosaccharides. (springer.com)
  • We previously used capillary isoelectric focusing (cIEF) with laser-induced-fluorescence detection to determine the experimental pI of individual mitochondria after their isolation under depolarizing conditions. (biomedsearch.com)
  • Other technologies such as capillary electrophoresis (CE) and capillary isoelectric focusing (cIEF) are also suggested. (chromatographyonline.com)
  • multicompartment electrolyzers for isoelectric membranes and membrane‐trapped enzyme reactors, and temperature‐programmed capillary zone electrophoresis (CZE). (wikipedia.org)
  • Here we demonstrate the ability to quickly resolve isoforms of three model proteins (bovine serum albumin, transferrin, α 1 -antitrypsin) in capillaries coated with novel dynamic coatings. (wiley.com)
  • Thus there are chapters on capillary gel electrophoresis, sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analysis of protein:protein interactions by gel electrophoresis. (nhbs.com)
  • Studies on the analytical performance of a non-covalent coating with N,N-didodecyl-N,N-dimethylammonium bromide for separation of basic proteins by capillary electrophoresis in acidic buffers in 25- and 50-microm capillaries. (nih.gov)
  • Various means are now known for fractionating substances such as proteins according to their charges and molecular weights by electrophoresis and isolating the obtained fractions, which include a fractionation capillary electrophoresis apparatus, a preparative liquid electrofocusing apparatus and a method in which electrical separation is conducted in a gel and thereafter a desired substance is extracted from the gel. (google.com)
  • Photochemical immobilization of proteins onto capillary walls following isoelectric focusing improves protein detection sensitivity. (sciencemag.org)
  • Proteins labeled with pyrylium dyes maintain their native charge and isoelectric points, which results in sharp and characteristic bands during electrophoresis. (activemotif.com)
  • Due to the unique conjugation mechanism, proteins labeled with Py-Dyes maintain their native charge and isoelectric point. (activemotif.com)
  • 5. Hypersensitive Measurement of Proteins by Capillary Isoelectric Focusing and Liquid Chromatography-Mass Spectrometry (Feng Zhou and Murray Johnston). (wiley.com)
  • LeBLANC, R.J. 1994-11-01 00:00:00 ABSTRACT A capillary zone electrophoresis (CZE) method for rapid separation of fish muscle sarcoplasmic proteins (SAJR) was developed using a highionic‐strength phosphate buffer and an untreated, fused silica capillary (20 μm i.d. (deepdyve.com)
  • Thomas also carries out efforts in instrumentation development, especially in the area of capillary electrophoresis-mass spectrometry (CE-MS). He uses said tools in order to elucidate the structure of proteins, e.g. (washington.edu)
  • In this paper, the potential of capillary electrophoresis as an alternative technique for the determination of pI values of both acidic and basic proteins was investigated. (nus.edu.sg)
  • Isoelectric focusing is one of the most powerful techniques used to solve complex mixtures of proteins [2]. (upo.es)
  • 4] K. B. Elkon, "Isoelectric focusing of human IgA and secretory proteins using thin layer agarose gels and nitrocellulose capillary blotting," J. Immunol. (upo.es)
  • Based on Beckman Coulters well-established automation and capillary electrophoresis technologies, the PA 800 addresses important issues in the characterization of a given proteome manipulating and resolving low levels of proteins that range from acidic to basic to membrane-bound. (bio-medicine.org)
  • On-line combination of capillary isoelectric focusing and capillary non-gel sieving electrophoresis using a hollow-fiber membrane interface: a novel two-dimensional separation system for proteins. (semanticscholar.org)
  • He has also worked on the editorial boards of the Journal of Chromatography, the Journal of Capillary Electrophoresis, the Journal of Biochemical and Biophysical Methods, BioTechniques, and the Journal of Proteomics. (wikipedia.org)
  • Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated mono- and oligosaccharides. (springer.com)
  • The metabolic profiles of brain biopsies obtained at surgery were recorded using capillary gas chromatography (GC). (nih.gov)
  • Integrated platform of capillary isoelectric focusing, trypsin immobilized enzyme microreactor and nanoreversed-phase liquid chromatography with mass spectrometry for online protein profiling[J]. ELECTROPHORESIS,2011,32(20):2848-2856. (dicp.ac.cn)
  • Capillary electrophoresis is again segmented into micellar electrokinetic capillary chromatography, capillary isoelectric focusing, capillary isotachophoresis and others. (medgadget.com)
  • He initially worked in private industry at a 'mom & pop' analytical laboratory and at J&W Scientific capillary gas chromatography column manufacturer (now part of Agilent) after earning his undergraduate degree at Humboldt State University (California). (asu.edu)
  • A special feature of the course is that all laboratory work will be performed on the same starting sample (native or recombinant Aequorea GFP), which will be purified from an exceedingly crude form to near homogeneity as judged by high performance liquid chromatography (HPLC), SDS gel electrophoresis, isoelectric focusing, and capillary zone electrophoresis. (bio.net)
  • Petersen, John R. / Clinical application of capillary isoelectric focusing on fused silica capillary for determination of hemoglobin variants . (utmb.edu)
  • High performance capillary electrophoresis is based on the differential migration of components of aqueous samples within a narrow fused silica capillary driven by an electric field. (environmental-expert.com)
  • Analysis of four therapeutic monoclonal antibodies by online capillary isoelectric focusing directly coupled to quadrupole time-of-flight mass spectrometry. (orphiz.be)
  • The combined use of the latter technique and capillary GC-mass spectrometry and pattern recognition analyses gives the possibility of the classification of diseased cells based solely on differences in their biochemical compositions. (nih.gov)
  • Chen, D. D. Y. Nonaqueous Capillary Electrophoresis Mass Spectrometry Method For Determining Highly Hydrophobic Peptides . (ubc.ca)
  • Intabio's Blaze System couples two gold-standard analytical techniques - imaged capillary isoelectric focusing (iCIEF) and high-resolution mass spectrometry (MS) - into a single 15-minute assay with 100 times the throughput of traditional workflows. (biospace.com)
  • Capillary isoelectric focusing and affinity capillary electrophoresis approaches for the determination of binding constants for antibodies to the prion protein. (uncg.edu)
  • Part of Beckman Coulters ProteomeLab family of products, the PA 800 offers a comprehensive range of automated protein characterization solutions including molecular weight determination, peptide mapping, isoelectric focusing, carbohydrate profiling and mechanisms to study protein/protein interactions in solution. (bio-medicine.org)
  • The chemical composition of the mitochondrial outer membrane is one of the factors defining the mitochondrial isoelectric point (pI), which is a property useful for the analysis and characterization of isolated mitochondria. (biomedsearch.com)
  • In particular, the application of capillary zone electrophoresis (CZE) for the characterization of HS has evoked an increased interest. (scielo.cl)
  • It presents the best methods, hints and tips for core procedures such as one- dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements and updates of the procedures. (nhbs.com)
  • Electrokinetic supercharging for highly efficient peptide preconcentration in capillary zone electrophoresis , in Electrophoresis, vol. 29, num. (epfl.ch)
  • Capillary Electrophoresis as a Second Dimension to Isoelectric Focusing for Peptide Separation , in Analytical Chemistry, vol. 79, num. (epfl.ch)
  • The isoelectric focusing (IEF) was performed without ampholytes and glycerol according to the manufacturer's instructions for peptide focusing. (thefreedictionary.com)
  • While MAM is a relatively new application of MS, it has the potential to replace multiple assays that are currently used in routine QC testing, including capillary electrophoresis, capillary isoelectric focusing, peptide mapping, glycan analysis, as well as others. (genengnews.com)
  • A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. (google.ca)
  • 2. The detection system of claim 1 wherein the capillaries have an inside diameter of about 20-500 microns and at least one optical fiber is coupled to each capillary in a manner to transmit the coherent light axially into the capillary. (google.ca)
  • 3. The detection system of claim 2 wherein the optical fiber is coupled to the capillary by insertion of the second end of the optical fiber into the outflow end of the capillary, the optical fiber being of a suitable diameter for affixing the fiber inside the capillary without substantially hindering the sample flow. (google.ca)
  • 4. The detection system of claim 1 wherein at least one optical fiber is coupled to each capillary and positioned adjacent to and orthogonally in relation to the capillary. (google.ca)
  • 5. The detection system of claim 1 further comprising a means for focusing light. (google.ca)
  • 6. The detection system of claim 5 wherein the focusing means is a lens for focusing fluorescent light emitted from the target species in the capillaries to the means for detecting fluorescent light. (google.ca)
  • 10. The detection system of claim 1 further comprising a spacer positioned between at least two capillaries to reduce optical crosstalk between the capillaries. (google.ca)
  • 13. The detection system of claim 1 wherein the array of capillaries comprises at least 100 capillaries. (google.ca)
  • 15. The detection system of claim 1 wherein at least one of the capillaries contains a gel that is suitable for capillary gel electrophoresis systems. (google.ca)
  • Lemma, T., Pawliszyn, J. Human serum albumin interaction with oxaliplatin studied by capillary isoelectric focusing with the whole column imaging detection and spectroscopic method. (uwaterloo.ca)
  • They also appear to inhibit protein adsorption to the capillary wall, making the isoform separations highly reproducible both in peak areas and apparent mobility. (wiley.com)
  • including (but not limited to) chromatographic techniques, capillary gel electrophoresis, isoelectric focusing, UV spectrophotometry. (thebigchoice.com)
  • In conventional isoelectric focusing techniques using microfluidic channels, complicated liquid manipulations are required, which can decrease analytical performance such as the reproducibility of a focusing position. (go.jp)
  • The isoelectric point (pI) of a protein is of practical importance in many separation procedures, both analytical and preparative. (nus.edu.sg)
  • Isoforms are traditionally analyzed using techniques such as gel- or capillary-based isoelectric focusing. (wiley.com)
  • However, with proper electroosmotic flow (EOF) control, isoforms with small p I differences can also be analyzed using capillary zone electrophoresis (CZE). (wiley.com)
  • Native capillary isoelectric focusing for the separation of protein complex isoforms and subcomplexes. (sigmaaldrich.com)
  • Schrötter S, Leondaritis G, Eickholt BJ (2016) Capillary Isoelectric Focusing of Akt Isoforms Identifies Highly Dynamic Phosphorylation in Neuronal Cells and Brain Tissue. (phosphosite.org)
  • Capel-205 is a new generation capillary electrophoresis system with a significantly extended autosampler for continuous running of dozens of analyses and increased efficiency in separation. (environmental-expert.com)
  • Capel-205 is a new generation capillary electrophoresis system with advanced options and design. (environmental-expert.com)
  • Reagent-release hydrogels containing an acid or a base solution were prepared and employed as ion suppliers in isoelectric focusing using short capillaries. (go.jp)
  • To develop a rapid and sensitive assay based on microfluidic isoelectric focusing, we previously prepared reagent-release capillaries retaining ampholytes by physical adsorption on the inner surface of a short capillary, which were then applied to isoelectric focusing. (go.jp)
  • In this study, reagent-release hydrogels containing an acid or a base solution were developed for isoelectric focusing using short capillaries in place of the acid and base solutions used in conventional microfluidic isoelectric focusing. (go.jp)
  • The prepared reagent-release hydrogels showed some advantages for isoelectric focusing, e.g. , high durability for at least five weeks of storage, easy handling as compared to the conventional solutions, and suppression of hydrodynamic flow in capillaries, which often decreases the reproducibility in capillary isoelectric focusing. (go.jp)
  • The migration behavior of ions from the hydrogels into the capillary and the formation of a pH gradient in isoelectric focusing using the reagent-release hydrogels were investigated, which confirmed that the prepared reagent-release hydrogels could form a similar pH gradient to that obtained with conventional capillary isoelectric focusing using the solutions. (go.jp)
  • Methods Upon mating the iCIEF instrumentation to the MS, a solution comprising 100?g/mL mAb in mobilizer was infused through the iCIEF\MS microchip less than 40C60 mbar of pressure Rabbit Polyclonal to ARPP21 GSK1521498 free base and electrosprayed into the Bruker Compact QTOF Mass Spec equipped with a NanoElectrospray capillary cap at a potential between ?3750 and ?4250 volts. (cp-724714.com)
  • Depending on the specific conditions of manufacturing or the innate properties of a mAb (such as pI), charge variant profiles can contain both acidic (lower isoelectric point species) or basic variants (higher isoelectric point species). (chromatographyonline.com)
  • They developed techniques for isoelectric focusing (IEF) using soluble carrier ampholytes and polyacrylamide gels. (wikipedia.org)
  • The PA 800 plus application menu includes SDS-gel molecular weight analysis, charge heterogeneity analysis utilizing advanced capillary isoelectric focusing technology, and carbohydrate profiling for the assessment of glycoprotein microheterogeneity. (labwrench.com)
  • Two methods of analysis are possible: (a) protein electrophoresis, a method of analyzing the composition of fluids, also known as "agarose gel electrophoresis/Coomassie Blue staining", and (b) the combination of isoelectric focusing/silver staining. (wikipedia.org)
  • Electrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. (selectscience.net)
  • Modeling the Isoelectric Focusing of Peptides in an OFFGEL Multicompartment Cell , in Journal of Proteome Research, vol. 6, num. (epfl.ch)
  • Capillary electrophoresis analysis of transferrin in human serum is used to assess genetic variants after desialylation with neuraminidase and iron saturation to reduce the complexity of the transferrin pattern and thus facilitate the recognition of transferrin polymorphisms. (uzh.ch)
  • Asialo-transferrin forms are analyzed by capillary zone electrophoresis using assay conditions as for the monitoring of carbohydrate-deficient transferrin or by capillary isoelectric focusing in a pH 5-8 gradient which requires immunoextraction of transferrin prior to analysis. (uzh.ch)
  • In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis (CE) for the analysis of complex carbohydrates. (springer.com)
  • Further, the diameter of the capillary is as small as 1 mm or less that the amount of the sample subjected to electrophoresis is so small as to bring about a drawback in that the apparatus is unsuitable for use in the step of electrophoresis requiring a large amount of sample in one operation as in the structural analysis of, for example, an amino acid sequence. (google.com)
  • Based on technology, the human identification analysis software market is segmented into polymerase chain reaction, purification and extraction, next-generation sequencing, microarray, automated liquid handling, capillary electrophoresis and DNA testing. (medgadget.com)
  • Congratulations to Lauren Ramsay, who defended her Ph.D. work "Ultrasensitive Capillary Isoelectric Focusing for the Analysis of Biological Samples" on April 1. (washington.edu)
  • Isoelectric focusing using capillary electrophoresis (CE) has also been used as a fast global method for charged variant analysis. (thermofisher.com)
  • Furthermore, strong correlation was observed between this novel MS approach and analysis by capillary isoelectric focusing. (genedata.com)
  • With the insights into how molecules are separated and detected in capillary columns, we wish to design novel instrument that can provide even more separation power for chemical analysis and purification. (ubc.ca)
  • The PA 800 plus Pharmaceutical Analysis system, the PA 800S plus provides an automated and quantitative solution for the analysis of protein purity and molecular weight utilizing SDS-gel capillary electrophoresis. (labwrench.com)
  • In this study, we have utilized affinity capillary electrophoresis (ACE), a form of capillary zone e. (uncg.edu)
  • In addition to applying this technology to many important biological problems, we have also developed software that allows us to visualize what is occurring in the capillary at every stage of an affinity separation, and to predict future results. (ubc.ca)
  • Monitoring of CC patterns revealed three cases, namely those producing double peaks in both methods, a double peak in capillary isoelectric focusing only and a double peak in capillary zone electrophoresis only. (uzh.ch)
  • His work on capillary zone electrophoresis (CZE) supports the identification of DNA point mutations. (wikipedia.org)
  • Slab electrophoresis is further segmented into zone electrophoresis, Isoelectric-focusing and Immune-electrophoresis. (sbwire.com)
  • The two capillary electrophoresis methods are shown to represent effective tools to assess unusual transferrin patterns, including genetic variants with dissimilar abundances of the two forms. (uzh.ch)
  • More complex biologics such as multi-specific antibodies introduce significant complexity, with the number of different charge variants-many of which can be undesirable from a safety and efficacy standpoint-increasing exponentially, according to Susan Darling, senior director for capillary electrophoresis (CE) and biopharma product marketing with SCIEX. (pharmtech.com)
  • Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods. (springer.com)
  • New techniques like "capillary isoelectric focusing immunoassay" are able to detect IgG OCBs in more than 95% of multiple sclerosis patients. (wikipedia.org)
  • In 2017, researchers stated "The work of P.G. Righetti has been paramount in the ability to separate biomolecules electrophoretically, particularly according to isoelectric point. (wikipedia.org)
  • Based on the electrophoresis types, clinical electrophoresis market is segmented into slab electrophoresis and capillary electrophoresis. (sbwire.com)
  • North America is expected to lead the global clinical electrophoresis market due to the stringent regulations regarding pharmaceutical manufacturing and focusing on research and development in diagnostics. (sbwire.com)
  • In Clinical Applications of Capillary Electrophoresis, Stephen Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. (springer.com)
  • Timely and highly practical, Capillary Electrophoresis of Carbohydrates provides both novice and experienced CE analysts with powerful tools for the successful separation of complex carbohydrates by capillary electrophoresis. (springer.com)
  • Emphasizing microseparations and microfluidics, the Handbook of Capillary and Microchip Electrophoresis, Third Edition features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. (routledge.com)
  • To prepare the RRC , covalent immobilization of poly(dimethylacrylamide) ( PDMA ) was conducted to suppress electro-osmotic flow (EOF), followed by physical adsorption of the mixture of carrier ampholyte (CA), surfactant , labeling reagent (LR), and other additives to the PDMA surface to construct a two-layer structure inside a square glass capillary. (rsc.org)
  • General approach for certain quantitative calculations for instance of the variance of reversible adsorption to the capillary wall in CE. (nih.gov)
  • In order to maintain their market shares, leading players are continuously focusing on developing innovative protein purification technologies and introducing new products in the market. (marketsandmarkets.com)
  • d) means for substantially simultaneously detecting changes in fluorescent emission by the target species through the annular wall of each capillary. (google.ca)
  • Additionally, the role of capillary electrophoresis (CE) has also been discussed toward amino acid separation. (springer.com)