A separation technique which combines LIQUID CHROMATOGRAPHY and CAPILLARY ELECTROPHORESIS.
A hybrid separation technique combining both chromatographic and electrophoretic separation principles. While the method was invented to separate neutral species, it can also be applied to charged molecules such as small peptides.
A phenethylamine that is an isomer of EPHEDRINE which has less central nervous system effects and usage is mainly for respiratory tract decongestion.
Compounds in which a methyl group is attached to the cyano moiety.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
The motion of a liquid through a membrane (or plug or capillary) consequent upon the application of an electric field across the membrane. (Oxford Dictionary of Biochemistry and Molecular Biology, 2001)

Study of transaldolase deficiency in urine samples by capillary LC-MS/MS. (1/44)

Transaldolase (TAL) is a key enzyme of the pentose phosphate pathway (PPP). TAL deficiency is a newly recognized cause of liver cirrhosis. We have developed an ion-pair LC separation combined with negative ion electrospray MS/MS detection method to assess PPP metabolites in urine samples from TAL-deficient mice. Sedoheptulose 7-phosphate (S7P), C5-polyols D-arabitol and D-ribitol, and 6-phosphogluconate (6PG) levels were markedly increased in urine of TAL-deficient mice with respect to those of wild-type and heterozygote littermates. The detection limits of S7P, D-arabitol, and 6PG were 0.15 +/- 0.015 pmol, 3.5 +/- 0.41 pmol, and 0.61 +/- 0.055 pmol, respectively. The limit of quantitation was 0.4 +/- 0.024 nmol/ml for S7P, 1.6 +/- 0.11 nmol/ml for 6PG and 10 +/- 0.7 nmol/ml for D-arabitol. Additional metabolites, hexose 6-phosphates (m/z 259), D-ribose 5-phosphate and D-xylulose 5-phosphate (m/z 229), D-fructose 1,6-diphosphate (m/z 339), C6-polyols (m/z 181) and GSSG (m/z 611), that have been positively identified in mouse urine, showed similar levels in control and TAL-deficient mice.  (+info)

Porous polyacrylamide monoliths in hydrophilic interaction capillary electrochromatography of oligosaccharides. (2/44)

Capillary electrochromatography (CEC) of oligosaccharides in porous polyacrylamide monoliths has been explored. While it is possible to alter separation capacity for various compounds by copolymerization of suitable separation ligands in the polymerization backbone, "blank" acrylamide matrix is also capable of sufficient resolution of oligosaccharides in the hydrophilic interaction mode. The "blank" acrylamide network, formed with a more rigid crosslinker, provides maximum efficiency for separations (routinely up to 350,000 theoretical plates/m for fluorescently-labeled oligosaccharides). These columns yield a high spatial resolution of the branched glycan isomers and large column permeabilities. From the structural point of view, some voids are observable in the monoliths at the mesoporous range (mean pore radius ca. 35 nm, surface area of 74 m2/g), as measured by intrusion porosimetry in the dry state.  (+info)

1-methyl-4-phenylpyridinium-induced alterations of glutathione status in immortalized rat dopaminergic neurons. (3/44)

Decreased glutathione levels associated with increased oxidative stress are a hallmark of numerous neurodegenerative diseases, including Parkinson's disease. GSH is an important molecule that serves as an anti-oxidant and is also a major determinant of cellular redox environment. Previous studies have demonstrated that neurotoxins can cause changes in reduced and oxidized GSH levels; however, information regarding steady state levels remains unexplored. The goal of this study was to characterize changes in cellular GSH levels and its regulatory enzymes in a dopaminergic cell line (N27) following treatment with the Parkinsonian toxin, 1-methyl-4-phenylpyridinium (MPP(+)). Cellular GSH levels were initially significantly decreased 12 h after treatment, but subsequently recovered to values greater than controls by 24 h. However, oxidized glutathione (GSSG) levels were increased 24 h following treatment, concomitant with a decrease in GSH/GSSG ratio prior to cell death. In accordance with these changes, ROS levels were also increased, confirming the presence of oxidative stress. Decreased enzymatic activities of glutathione reductase and glutamate-cysteine ligase by 20-25% were observed at early time points and partly account for changes in GSH levels after MPP(+) exposure. Additionally, glutathione peroxidase activity was increased 24 h following treatment. MPP(+) treatment was not associated with increased efflux of glutathione to the medium. These data further elucidate the mechanisms underlying GSH depletion in response to the Parkinsonian toxin, MPP(+).  (+info)

Analysis of [3',3'-d(2)]-nicotine and [3',3'-d(2)]-cotinine by capillary liquid chromatography-electrospray tandem mass spectrometry. (4/44)

A selective and sensitive LC/MS/MS assay was developed for the quantification of d(2)-nicotine and d(2)-cotinine in plasma of current and past smokers administered d(2)-nicotine. After solid phase extraction and liquid-liquid extraction, HPLC separation was achieved on a capillary hydrophilic interaction chromatography phase column. The analytes were monitored by tandem mass spectrometry with electrospray positive ionization. Linear calibration curves were generated for d(2)-nicotine (0.03-6.0 ng/ml plasma) and d(2)-cotinine (0.15-25 ng/ml plasma). The lower limits of quantitation were 0.15 ng/ml and 0.25 ng/ml for d(2)-nicotine and d(2)-cotinine, respectively. The coefficient of variation was 3.7% for d(2)-nicotine and 2.5% for d(2)-cotinine. The method was applied to two ongoing studies of d(2)-nicotine metabolism in prior and current smokers. Preliminary analysis of a subset of subjects from these studies detected a significantly lower rate of nicotine conversion to cotinine by past smokers compared to current smokers.  (+info)

Component and antioxidant properties of polysaccharide fractions isolated from Angelica sinensis (OLIV.) DIELS. (5/44)

An analytical method of high performance capillary electrophoresis (HPCE) was developed to simultaneously separate and identify the component monosaccharides of Angelica sinensis polysaccharide fractions (APFs), named APF1, APF2 and APF3. The predominant sugars in APFs were identified as arabinose, glucose, rhamnose, galactose and galacturonic acid as well as trace amount of mannose and glucuronic acid, and the fractionation altered significantly the distribution of component monosaccharides in APFs. APF3 was the most active fraction to effectively inhibit H(2)O(2)-caused decrease of cell viability, lactate dehydrogenase (LDH) leakage and malondialdehyde (MDA) formation, and also reduced H(2)O(2)-caused decline of superoxide dismutase (SOD) activity and glutathione (GSH) depletion (p<0.05), followed by APF2 and APF1 in decreasing order. Furthermore, it was found that APFs (100 microg/ml) could protect macrophages by inhibiting the release of excess NO and reactive oxygen species (ROS) induced by high concentrations of H(2)O(2) (0.8-1.6 mM).  (+info)

Effect of decreasing column inner diameter and use of off-line two-dimensional chromatography on metabolite detection in complex mixtures. (6/44)

Capillary liquid chromatography coupled with electrospray ionization to a quadrupole ion trap mass spectrometer was explored as a method for the analysis of polar anionic compounds in complex metabolome mixtures. A ternary mobile phase gradient, consisting of aqueous acidic, aqueous neutral and organic phases in combination with an aqueous compatible reversed-phase stationary phase allowed metabolites with a wide range of polarities to be resolved and detected. Detection limits in the full scan mode for glycolysis and tricarboxylic acid cycle intermediates were from 0.9 to 36fmol. Using this system, 111+/-9 (n=3) metabolites were detected in Escherichia coli lysate samples. Reducing column I.D. from 50 to 25microm increased the number of metabolites detected to 156+/-17 (n=3). The improvement in number of metabolites detected was attributed to an increase in separation efficiency, an increase in sensitivity, and a decrease in adduct formation. Implementation of a second separation mode, strong anion exchange, to fractionate the sample prior to capillary RPLC increased the number of metabolites detected to 244+/-21 (n=3). This improvement was attributed to the increased peak capacity which decreased co-elution of molecules enabling more sensitive detection by mass spectrometry. This system was also applied to islets of Langerhans where more significant improvements in metabolite detection were observed. In islets, 391+/-33 small molecules were detected using the two-dimensional separation. The results demonstrate that column miniaturization and use of two-dimensional separations can yield a significant improvement in the coverage of the metabolome.  (+info)

Evaluation of polymeric methacrylate-based monoliths in capillary electrochromatography for their potential to separate pharmaceutical compounds. (7/44)

Polymeric methacrylate-based monoliths are evaluated in capillary electrochromatography (CEC) and pressurized capillary electrochromatography (p-CEC) for their potential in pharmaceutical analysis. Using a given polymerization mixture as a basis for the monolith synthesis, different mobile phase pH at constant organic modifier concentrations are tested in both CEC and p-CEC. The test set consists of basic, acidic, amphoteric, and neutral compounds, which are mainly pharmaceuticals. Because of the mainly hydrophobic character of the stationary phase, the interactions are largest when the compounds appear in an uncharged state, but some ion-exchange phenomena with negatively charged compounds can also be observed. In CEC, acidic substances are most retained at low pH. For amphoteric and neutral compounds, no preference regarding analyzing pH can be derived from these experiments. For basics, a high pH is chosen, but a reduced solvent strength is needed to enhance the retention of these compounds. The retention mechanism in p-CEC can also be assigned to both hydrophobic and ionic interactions. For acidic, amphoteric, and neutral compounds, acceptable retention is seen. For the basic compounds, the retention with a mobile phase containing 50% organic modifier is low, as in CEC. However, when the organic modifier content in the mobile phase is decreased, retention increases and the selectivity of the stationary phase is more pronounced. This mode of operation presents a possibility for separating some test mixtures, thus some potential for pharmaceutical analysis is seen. More efforts are needed to obtain higher efficiencies and better peak shapes, which might be solved by a further optimization of both the stationary phase synthesis and the mobile phase composition.  (+info)

A novel chip device based on wired capillary packed with high performance polymer-based monolith for HPLC: reproducibility in preparation processes to obtain long columns. (8/44)

This report describes the development of novel wired chip devices for mu-HPLC analyses. The monolithic capillary column to be wired was prepared using a tri-functional epoxy monomer, tris(2,3-epoxypropyl)isocyanurate with a diamine, 4-[(4-aminocyclohexyl)methyl]cyclohexylamine. The prepared column was evaluated by SEM observation of the sectional structure of column and micro-HPLC. In addition, the reproducibility in the preparation of long capillary columns having nearly 1 m length was extensively examined for applications of novel wired chip devices. The authors demonstrated that the monolithic structure of the prepared long capillary could be finely controlled under the strictly maintained operational conditions and thus the relative standard deviation (RSD) of the column properties such as the number of theoretical plates, retention factor, and permeability could be well controlled to become less than 10%. Furthermore, the wired chip device column showed that its high performance was kept even after chip preparation.  (+info)

In capillary electrochromatography capillaries, packed with HPLC stationary phase, are subjected to a high voltage. Separation ... Capillary electrochromatography (CEC) combines the principles used in HPLC and CE. The mobile phase is driven across the ... Capillary electrochromatography (CEC) is a chromatographic technique in which the mobile phase is driven through the ... Capillary electrochromatography is a combination of two analytical techniques, high-performance liquid chromatography and ...
Capillary electrochromatography (CEC) is an electrochromatography technique in which the liquid mobile phase is driven through ... Cikalo, Maria G.; Bartle, Keith D.; Robson, Mark M.; Myers, Peter; Euerby, Melvin R. (1998). "Capillary electrochromatography ... Dittmann, Monika M.; Rozing, Gerard P. (1996). "Capillary electrochromatography - a high-efficiency micro-separation technique ... It is a combination of high-performance liquid chromatography and capillary electrophoresis. The capillaries is packed with ...
"Optimized separation of pharmacologically active anthraquinones in Rhubarb by capillary electrochromatography". Electrophoresis ...
"Optimized separation of beta-blockers with multiple chiral centers using capillary electrochromatography-mass spectrometry". J ...
"Analysis of oxygen heterocyclic compounds in citrus essential oils by capillary electrochromatography and comparison with HPLC ...
It may be a liquid (LC and capillary electrochromatography (CEC)), a gas (GC), or a supercritical fluid (supercritical-fluid ... Further, capillary columns can be split into three classes: porous layer open tubular (PLOT), wall-coated open tubular (WCOT) ... Capillary columns generally give far superior resolution and although more expensive are becoming widely used, especially for ... Stainless steel and glass are the usual materials for packed columns and quartz or fused silica for capillary columns. Gas ...
... high temperature chromatography and capillary electrochromatography. Western blotting can be used in order to quantify the ... Some newer methods for the separation and identification of proteins include the use of monolithic capillary columns, ...
By taking advantage of the capillary effects of the small carbon nanotubes, it is possible to condense gases in high density ... based on electrochromatography. Here, the high surface-area-to-volume ratio and high hydrophobicity of CNTs are used in order ... "Carbon nanotube based separation columns for high electrical field strengths in microchip electrochromatography". Lab on a Chip ...
Capillary electrochromatography; Monolithic capillary; Nano spherical covalent organic polymers; Sulfonic acid ... Capillary electrochromatography (CEC) monolithic columns were fabricated using the physical doping technique to exhibit the ... Sulfonic acid functionalized monolithic column for high selectivity capillary electrochromatography separation. Dai, Shuyu; Guo ... Sulfonic acid functionalized monolithic column for high selectivity capillary electrochrom ...
capillary electrochromatography separation technique electroosmosis Open-tubular capillary electrochromatography poly( ... Investigation of the stability of polyelectrolyte multilayer coatings in open-tubular capillary electrochromatography using ... and strong stabilities against extreme pH values when used in open-tubular capillary electrochromatography (OT-CEC). In this ... Chromatography, Micellar Electrokinetic Capillary 1,1 binaphthyl 2,2 dihydrogenphosphate 1,1 binaphthyl derivative chemical ...
Ganzera, M. (2011): Capillary electrophoresis and capillary electrochromatography - suitable for quality control and routine ... Ganzera, M. (2013): Natural products analysis by capillary electrophoresis and capillary electrochromatography. Invited ... amara by capillary electrochromatography using a novel monolithic stationary phase. Journal of Separation Science, 24, 2301- ... Ganzera, M., Nischang, I. (2010): The use of capillary electrochromatography for natural products analysis - theoretical ...
Capillary Electrochromatography. Capillary Endothelium use Endothelium, Vascular. Capillary Fragility. Capillary Leak Syndrome ...
Flexible separation modes including capillary electrochromatography. *Easy maintenance and automated instrument diagnostics ... Diode-array detection in capillary electrophoresis using Agilent Extended Light Path capillaries - Protected (PDF, 525 KB) ... Capillary Electrophoresis (CE) offers fast separations with exceptional efficiency and resolution for analytical challenges ...
... capillary electrochromatography; SFC: super critical fluid chromatography. ... HPLC: high-performance liquid chromatography; LC: liquid chromatography; GC: gas chromatography; CE: capillary electrophoresis ...
Capillary Electrochromatography / methods Actions. * Search in PubMed * Search in MeSH * Add to Search ... Capillary electrophoresis analysis of nitric oxide synthase related metabolites in single identified neurons. Floyd PD, Moroz ... Using capillary electrophoresis with laser-induced fluorescence detection, ArgSuc was found to be present in the nervous system ... Enhanced single-cell metabolomics by capillary electrophoresis electrospray ionization-mass spectrometry with field amplified ...
Chiral separation by capillary electrochromatography.. Gübitz G; Schmid MG. Enantiomer; 2000; 5(1):5-11. PubMed ID: 10763864. [ ... 2. Enantioseparations in capillary electrochromatography using sulfated poly β-cyclodextrin-modified silica-based monolith as ... Open tubular molecular imprinted phases in chiral capillary electrochromatography.. Cheong WJ; Yang SH. Methods Mol Biol; 2013 ... 8. Application of chiral ligand-exchange stationary phases in capillary electrochromatography.. Schmid MG. Methods Mol Biol; ...
A novel imidazolium-based organic-silica hybrid monolith for per aqueous capillary electrochromatography. R. Soc. Chem. Adv. ...
2011), affinity chromatography, gel filtration chromatography and capillary electrochromatography (Cheng and Huang 2004; Babu ... attempt of self-protection which takes into account the discharge of various factors that causes the distention of capillary ... mediators and anti-inflammatory prostaglandins as a result of which the distention and permeability of capillaries is reduced ( ...
Capillary electrophoresis of drugs: current status in the analysis of pharmaceuticals. Download Prime PubMed App to iPhone, ... Applications of capillary electrochromatography.. *Recent progress in capillary electrophoretic analysis of amino acid ... Pharmaceutical and biomedical applications of chiral capillary electrophoresis and capillary electrochromatography: an update. ... Why not using capillary electrophoresis in drug analysis?. *Nonaqueous capillary electrophoresis--its applicability in the ...
Capillary_Electrophoresis_and_Electrochromatography : property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+ ...
Electrophoresis, Capillary [E05.196.401.190] * Capillary Electrochromatography [E05.196.401.190.249] * Electrophoresis, ... Chip Electrochromatography Electrochromatography, Capillary Public MeSH Note. 2007. History Note. 2007. Date Established. 2007/ ... Capillary Electrochromatography Preferred Term Term UI T670267. Date03/31/2006. LexicalTag NON. ThesaurusID NLM (2007). ... Chip Electrochromatography Narrower Concept UI. M0496570. Terms. Chip Electrochromatography Preferred Term Term UI T670269. ...
Accessories: I noted 31 new accessories for LC, GC, capillary electrophoresis (CE) and capillary electrochromatography (CEC), ... The columns will be available with nano- (50-75-μm), capillary (0.15-0.75 mm), microbore (1.0-2.1 mm), and conventional (4.6 mm ... Previously, the company introduced a capillary version (0.1-mm i.d.), in analytical version (4.6-mm i.d.) and preparative ... Chiral Technologies has expanded their popular Chiralpak phases to include capillary dimensions. Because supercritical fluid ...
Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography. MES is often ...
... monolithic stationary phases prepared in polyacrylate-coated fused-silica capillaries for capillary electrochromatography ...
Electrophoresis, Capillary [E05.196.401.190] * Capillary Electrochromatography [E05.196.401.190.249] * Electrophoresis, ... Chip Electrochromatography Electrochromatography, Capillary Public MeSH Note. 2007. History Note. 2007. Date Established. 2007/ ... Capillary Electrochromatography Preferred Term Term UI T670267. Date03/31/2006. LexicalTag NON. ThesaurusID NLM (2007). ... Chip Electrochromatography Narrower Concept UI. M0496570. Terms. Chip Electrochromatography Preferred Term Term UI T670269. ...
... yes capillary electrochromatographic,adj,E0748130,capillary electrochromatography,noun,E0430530,yes sialoendoscopic,adj, ... yes capillary gas chromatographic,adj,E0747788,capillary gas chromatography,noun,E0662431,yes apomorphic,adj,E0603368,apomorphy ... electrochromatography,noun,E0438995,yes micrometric,adj,E0317065,micrometry,noun,E0335310,yes dynamometric,adj,E0317824, ...
Fritless packed columns with great potential for use in capillary electrochromatography C. Fujimoto ...
capillary electrochromatography. Capillary electrochromatography (CEC) is a hybrid technique in which capillary columns are ... capillary zone electrophoresis. Capillary zone electrophoresis (CZE) is an electrophoretic separation technique using an ... capillary isotachophoesis (CITP). The main feature of CITP is that it is performed in a discontinuous buffer system. Sample ... capillary gel electrophoresis. The main separation mechanism in CGE is based on differences in solute size as analytes migrate ...
Capillary Electrochromatographies. Chip Electrochromatography. Electrochromatography, Capillary. Electrochromatography, Chip. ... Capillary Electrochromatography - Preferred Concept UI. M0496569. Scope note. A separation technique which combines LIQUID ... A separation technique which combines LIQUID CHROMATOGRAPHY and CAPILLARY ELECTROPHORESIS.. Allowable Qualifiers:. CL ... Capillary Electrochromatography Entry term(s). Capillary Electrochromatographies Electrochromatography, Capillary Chip ...
Separation of amino acids by open tubular capillary electrochromatography in fused silica capillaries with inner surface ... Separation of peptides by capillary zone electrophoresis with regulation of electroosmotic flow by radial electric field ... Analytical and preparative separations of biologically active peptides by capillary and free-flow electromigration methods ... Analysis and characterization of phosphinic pseudopeptides by capillary zone electrophoresis ...
Article Title: Triazine herbicide imprinted monolithic column for capillary electrochromatography.. Publication date: 2015. ...
Chao, Chiayu Helen, "Dendritic Polymers as Bonded Stationary Phases in Capillary Electrochromatography" (2000). Seton Hall ...
Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos. J ... "Electrophoresis, Capillary" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH ( ... Capillary Electrophoresis-Mass Spectrometry at Trial by Metabo-Ring: Effective Electrophoretic Mobility for Reproducible and ... This graph shows the total number of publications written about "Electrophoresis, Capillary" by people in Harvard Catalyst ...
More information about capillary electrophoresis method of analysis ... Among them are capillary gel electrophoresis, capillary isoelectric focusing, and capillary electrochromatography. ... In capillary electrophoresis (CE), a capillary is usually filled with an appropriate buffer. A sample solution is injected from ... The presence of ionizable groups (- Si - OH) on the inner surface of the fused silica capillary gives rise to a phenomenon ...
... a capillary tube, a detector and an output device. Capillary electrochromatography - It is a combination of capillary ... Basic schematic of a capillary GC column-with an outer diameter of a capillary GC an. Cec with some columns, and capillaries ... Within the capillary, current flows through the capillary, current flows through capillary! Electrophoresis systems in ... Referred as high performance capillary electrophoresis (HPCE), capillary zone electrophoresis (CZE), Free solution capillary ...
Capillary electrochromatography of sterols and related steryl esters derived from vegetable oils.. Abidi SL, J Chromatogr A ... Capillary liquid chromatographic analysis of fat-soluble vitamins and b-carotene in combination with in-tube solid-phase ... Assessing vitamin D nutritional status: Is capillary blood adequate?. Jensen M.E. et al., Clin Chim Acta 2016, 457, 59-62 ... Capillary electrochromatographic evaluation of vitamin E-active oil constituents : tocopherols and tocotrienols.. Abidi SL et ...
  • Electrophoretic separation technique performed in a small fused silica capillary. (speciation.net)
  • The presence of ionizable groups (- Si - OH) on the inner surface of the fused silica capillary gives rise to a phenomenon called electroosmotic flow, which is just a bulk flow of the whole liquid inside the capillary occurring when the voltage is applied. (lumexinstruments.es)
  • High-Sensitivity Glycan Profiling of Blood-Derived Immunoglobulin G, Plasma, and Extracellular Vesicle Isolates with Capillary Zone Electrophoresis-Mass Spectrometry. (harvard.edu)
  • Capillary Electrophoresis-Mass Spectrometry at Trial by Metabo-Ring: Effective Electrophoretic Mobility for Reproducible and Robust Compound Annotation. (harvard.edu)
  • Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos. (harvard.edu)
  • Analysis of hydrophobic and/or electrically neutral drugs by electrokinetic chromatography, capillary electrochromatography and nonaqueous CE is critically evaluated. (unboundmedicine.com)
  • The company therefore has a strong and dynamic research group which is particularly active in capillary LC, electrochromatography and the development of new chiral stationary phases. (capitalhplc.com)
  • A chiral separation mannequin of gel electrochromatography in a polydimethylsiloxane (PDMS) microfluidic system for amino acids (AAs) is introduced. (teitell-lab.com)
  • Capillary Electrophoresis (CE) offers fast separations with exceptional efficiency and resolution for analytical challenges that often can only be met with difficulty by LC. (dksh.com)
  • 188. Cs.Filep, A.Guttman, Electromigration Dispersion in Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins, Anal.Chem . (hlbs.org)
  • technique has the surfacemediated selectivity potential of HPLC and the high efficiency of capillary electrophoresis (CE). (speciation.net)
  • On-Chip Preconcentration Microchip Capillary Electrophoresis Based CE-PRM-LIVE for High-Throughput Selectivity Profiling of Deubiquitinase Inhibitors. (harvard.edu)
  • Capillary zone electrophoresis (CZE) is an electrophoretic separation technique using an electrolyte-filled capillary. (speciation.net)
  • When an electric field is applied across the capillary, ionic species migrate towards the oppositely charged electrode with a linear velocity depending on the electrophoretic mobility of the species in the electrolyte. (speciation.net)
  • A sample solution is injected from the inlet end of the capillary, electric field is applied to the capillary ends, and components migrate to the detector point with different velocities due to distinctions in their electrophoretic properties. (lumexinstruments.es)
  • Because of their solvation, the ion movement drags the bulk solution in the capillary towards the cathode resulting in an electroosmotic flow. (speciation.net)
  • ELECTROPHORESIS will continue to serve as an indispensable vehicle for the dissemination of efficacious advances by covering all operative approaches from gels through capillaries to chips. (wiley-vch.de)
  • Electrophoresis, Capillary" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • 6. Simultaneous determination of four tanshinones in Salvia miltiorrhiza by pressurized liquid extraction and capillary electrochromatography. (nih.gov)
  • The products on offer include specially coated capillaries, packed columns for CEC, CE reagents including numerous cyclodextrins and many other accessories. (capitalhplc.com)
  • We describe the use of capillary zone electrophoresis (CZE) for the qualitative and quantitative determination of major alkaloids (i.e., thebaine, codeine, morphine, papavarine and narcotine) in gum opium involving the analysis of alkaloids without derivatization or purification. (nih.gov)
  • An EKC methodology for the willpower of ornithine (Orn) enantiomers has been developed after a quick pre-capillary derivatization with FITC. (teitell-lab.com)
  • Nishi, H. "Capillary Electrophoresis of Drugs: Current Status in the Analysis of Pharmaceuticals. (unboundmedicine.com)
  • TY - JOUR T1 - Capillary electrophoresis of drugs: current status in the analysis of pharmaceuticals. (unboundmedicine.com)
  • They serve as anti-convective media, minimize solute diffusion, which contributes to zone broadening, prevent solute adsorption to the capillary walls and they help to eliminate electroosmosis. (speciation.net)
  • A novel Au-Fe3O4 nanoparticles-based capillary column was fabricated by magnetic approach for open-tubular capillary electrochromatography (OT-CEC). (sagepub.com)
  • This mode of operation is therefore different from other modes of capillary electrophoresis, eg CZE, which are normally carried out in a uniform carrier buffer and is characterized by sample zones which continuously change shape and relative position. (speciation.net)
  • Below are the most recent publications written about "Electrophoresis, Capillary" by people in Profiles. (harvard.edu)
  • 21. High-efficiency liquid chromatography-mass spectrometry separations with 50 mm, 250 mm, and 1 m long polymer-based monolithic capillary columns for the characterization of complex proteolytic digests. (nih.gov)
  • 36. Periodic imidazolium-bridged hybrid monolith for high-efficiency capillary liquid chromatography with enhanced selectivity. (nih.gov)
  • T) in 23 samples which had a Hb A 2 variant peak in zone 1 of the capillary electrophoresis (CE) electropherogram. (nih.gov)
  • 22. Monolithic poly(1,2-bis(p-vinylphenyl)ethane) capillary columns for simultaneous separation of low- and high-molecular-weight compounds. (nih.gov)