Study of transaldolase deficiency in urine samples by capillary LC-MS/MS. (1/44)

Transaldolase (TAL) is a key enzyme of the pentose phosphate pathway (PPP). TAL deficiency is a newly recognized cause of liver cirrhosis. We have developed an ion-pair LC separation combined with negative ion electrospray MS/MS detection method to assess PPP metabolites in urine samples from TAL-deficient mice. Sedoheptulose 7-phosphate (S7P), C5-polyols D-arabitol and D-ribitol, and 6-phosphogluconate (6PG) levels were markedly increased in urine of TAL-deficient mice with respect to those of wild-type and heterozygote littermates. The detection limits of S7P, D-arabitol, and 6PG were 0.15 +/- 0.015 pmol, 3.5 +/- 0.41 pmol, and 0.61 +/- 0.055 pmol, respectively. The limit of quantitation was 0.4 +/- 0.024 nmol/ml for S7P, 1.6 +/- 0.11 nmol/ml for 6PG and 10 +/- 0.7 nmol/ml for D-arabitol. Additional metabolites, hexose 6-phosphates (m/z 259), D-ribose 5-phosphate and D-xylulose 5-phosphate (m/z 229), D-fructose 1,6-diphosphate (m/z 339), C6-polyols (m/z 181) and GSSG (m/z 611), that have been positively identified in mouse urine, showed similar levels in control and TAL-deficient mice.  (+info)

Porous polyacrylamide monoliths in hydrophilic interaction capillary electrochromatography of oligosaccharides. (2/44)

Capillary electrochromatography (CEC) of oligosaccharides in porous polyacrylamide monoliths has been explored. While it is possible to alter separation capacity for various compounds by copolymerization of suitable separation ligands in the polymerization backbone, "blank" acrylamide matrix is also capable of sufficient resolution of oligosaccharides in the hydrophilic interaction mode. The "blank" acrylamide network, formed with a more rigid crosslinker, provides maximum efficiency for separations (routinely up to 350,000 theoretical plates/m for fluorescently-labeled oligosaccharides). These columns yield a high spatial resolution of the branched glycan isomers and large column permeabilities. From the structural point of view, some voids are observable in the monoliths at the mesoporous range (mean pore radius ca. 35 nm, surface area of 74 m2/g), as measured by intrusion porosimetry in the dry state.  (+info)

1-methyl-4-phenylpyridinium-induced alterations of glutathione status in immortalized rat dopaminergic neurons. (3/44)

Decreased glutathione levels associated with increased oxidative stress are a hallmark of numerous neurodegenerative diseases, including Parkinson's disease. GSH is an important molecule that serves as an anti-oxidant and is also a major determinant of cellular redox environment. Previous studies have demonstrated that neurotoxins can cause changes in reduced and oxidized GSH levels; however, information regarding steady state levels remains unexplored. The goal of this study was to characterize changes in cellular GSH levels and its regulatory enzymes in a dopaminergic cell line (N27) following treatment with the Parkinsonian toxin, 1-methyl-4-phenylpyridinium (MPP(+)). Cellular GSH levels were initially significantly decreased 12 h after treatment, but subsequently recovered to values greater than controls by 24 h. However, oxidized glutathione (GSSG) levels were increased 24 h following treatment, concomitant with a decrease in GSH/GSSG ratio prior to cell death. In accordance with these changes, ROS levels were also increased, confirming the presence of oxidative stress. Decreased enzymatic activities of glutathione reductase and glutamate-cysteine ligase by 20-25% were observed at early time points and partly account for changes in GSH levels after MPP(+) exposure. Additionally, glutathione peroxidase activity was increased 24 h following treatment. MPP(+) treatment was not associated with increased efflux of glutathione to the medium. These data further elucidate the mechanisms underlying GSH depletion in response to the Parkinsonian toxin, MPP(+).  (+info)

Analysis of [3',3'-d(2)]-nicotine and [3',3'-d(2)]-cotinine by capillary liquid chromatography-electrospray tandem mass spectrometry. (4/44)

A selective and sensitive LC/MS/MS assay was developed for the quantification of d(2)-nicotine and d(2)-cotinine in plasma of current and past smokers administered d(2)-nicotine. After solid phase extraction and liquid-liquid extraction, HPLC separation was achieved on a capillary hydrophilic interaction chromatography phase column. The analytes were monitored by tandem mass spectrometry with electrospray positive ionization. Linear calibration curves were generated for d(2)-nicotine (0.03-6.0 ng/ml plasma) and d(2)-cotinine (0.15-25 ng/ml plasma). The lower limits of quantitation were 0.15 ng/ml and 0.25 ng/ml for d(2)-nicotine and d(2)-cotinine, respectively. The coefficient of variation was 3.7% for d(2)-nicotine and 2.5% for d(2)-cotinine. The method was applied to two ongoing studies of d(2)-nicotine metabolism in prior and current smokers. Preliminary analysis of a subset of subjects from these studies detected a significantly lower rate of nicotine conversion to cotinine by past smokers compared to current smokers.  (+info)

Component and antioxidant properties of polysaccharide fractions isolated from Angelica sinensis (OLIV.) DIELS. (5/44)

An analytical method of high performance capillary electrophoresis (HPCE) was developed to simultaneously separate and identify the component monosaccharides of Angelica sinensis polysaccharide fractions (APFs), named APF1, APF2 and APF3. The predominant sugars in APFs were identified as arabinose, glucose, rhamnose, galactose and galacturonic acid as well as trace amount of mannose and glucuronic acid, and the fractionation altered significantly the distribution of component monosaccharides in APFs. APF3 was the most active fraction to effectively inhibit H(2)O(2)-caused decrease of cell viability, lactate dehydrogenase (LDH) leakage and malondialdehyde (MDA) formation, and also reduced H(2)O(2)-caused decline of superoxide dismutase (SOD) activity and glutathione (GSH) depletion (p<0.05), followed by APF2 and APF1 in decreasing order. Furthermore, it was found that APFs (100 microg/ml) could protect macrophages by inhibiting the release of excess NO and reactive oxygen species (ROS) induced by high concentrations of H(2)O(2) (0.8-1.6 mM).  (+info)

Effect of decreasing column inner diameter and use of off-line two-dimensional chromatography on metabolite detection in complex mixtures. (6/44)

Capillary liquid chromatography coupled with electrospray ionization to a quadrupole ion trap mass spectrometer was explored as a method for the analysis of polar anionic compounds in complex metabolome mixtures. A ternary mobile phase gradient, consisting of aqueous acidic, aqueous neutral and organic phases in combination with an aqueous compatible reversed-phase stationary phase allowed metabolites with a wide range of polarities to be resolved and detected. Detection limits in the full scan mode for glycolysis and tricarboxylic acid cycle intermediates were from 0.9 to 36fmol. Using this system, 111+/-9 (n=3) metabolites were detected in Escherichia coli lysate samples. Reducing column I.D. from 50 to 25microm increased the number of metabolites detected to 156+/-17 (n=3). The improvement in number of metabolites detected was attributed to an increase in separation efficiency, an increase in sensitivity, and a decrease in adduct formation. Implementation of a second separation mode, strong anion exchange, to fractionate the sample prior to capillary RPLC increased the number of metabolites detected to 244+/-21 (n=3). This improvement was attributed to the increased peak capacity which decreased co-elution of molecules enabling more sensitive detection by mass spectrometry. This system was also applied to islets of Langerhans where more significant improvements in metabolite detection were observed. In islets, 391+/-33 small molecules were detected using the two-dimensional separation. The results demonstrate that column miniaturization and use of two-dimensional separations can yield a significant improvement in the coverage of the metabolome.  (+info)

Evaluation of polymeric methacrylate-based monoliths in capillary electrochromatography for their potential to separate pharmaceutical compounds. (7/44)

Polymeric methacrylate-based monoliths are evaluated in capillary electrochromatography (CEC) and pressurized capillary electrochromatography (p-CEC) for their potential in pharmaceutical analysis. Using a given polymerization mixture as a basis for the monolith synthesis, different mobile phase pH at constant organic modifier concentrations are tested in both CEC and p-CEC. The test set consists of basic, acidic, amphoteric, and neutral compounds, which are mainly pharmaceuticals. Because of the mainly hydrophobic character of the stationary phase, the interactions are largest when the compounds appear in an uncharged state, but some ion-exchange phenomena with negatively charged compounds can also be observed. In CEC, acidic substances are most retained at low pH. For amphoteric and neutral compounds, no preference regarding analyzing pH can be derived from these experiments. For basics, a high pH is chosen, but a reduced solvent strength is needed to enhance the retention of these compounds. The retention mechanism in p-CEC can also be assigned to both hydrophobic and ionic interactions. For acidic, amphoteric, and neutral compounds, acceptable retention is seen. For the basic compounds, the retention with a mobile phase containing 50% organic modifier is low, as in CEC. However, when the organic modifier content in the mobile phase is decreased, retention increases and the selectivity of the stationary phase is more pronounced. This mode of operation presents a possibility for separating some test mixtures, thus some potential for pharmaceutical analysis is seen. More efforts are needed to obtain higher efficiencies and better peak shapes, which might be solved by a further optimization of both the stationary phase synthesis and the mobile phase composition.  (+info)

A novel chip device based on wired capillary packed with high performance polymer-based monolith for HPLC: reproducibility in preparation processes to obtain long columns. (8/44)

This report describes the development of novel wired chip devices for mu-HPLC analyses. The monolithic capillary column to be wired was prepared using a tri-functional epoxy monomer, tris(2,3-epoxypropyl)isocyanurate with a diamine, 4-[(4-aminocyclohexyl)methyl]cyclohexylamine. The prepared column was evaluated by SEM observation of the sectional structure of column and micro-HPLC. In addition, the reproducibility in the preparation of long capillary columns having nearly 1 m length was extensively examined for applications of novel wired chip devices. The authors demonstrated that the monolithic structure of the prepared long capillary could be finely controlled under the strictly maintained operational conditions and thus the relative standard deviation (RSD) of the column properties such as the number of theoretical plates, retention factor, and permeability could be well controlled to become less than 10%. Furthermore, the wired chip device column showed that its high performance was kept even after chip preparation.  (+info)

*Nadolol

"Optimized separation of beta-blockers with multiple chiral centers using capillary electrochromatography-mass spectrometry". J ...

*5-Geranyloxy-7-methoxycoumarin

"Analysis of oxygen heterocyclic compounds in citrus essential oils by capillary electrochromatography and comparison with HPLC ...

*Bergamot essential oil

"Analysis of oxygen heterocyclic compounds in citrus essential oils by capillary electrochromatography and comparison with HPLC ... "Multidimensional Capillary GC−GC for the Analysis of Complex Samples. 5. Enantiomeric Distribution of Monoterpene Hydrocarbons ... added reconstituted bergamot oil in genuine bergamot essential oil by high resolution gas chromatography with chiral capillary ...

*Chromatography

It may be a liquid (LC and Capillary Electrochromatography (CEC)), a gas (GC), or a supercritical fluid (supercritical-fluid ... Capillary columns generally give far superior resolution and although more expensive are becoming widely used, especially for ... Stainless steel and glass are the usual materials for packed columns and quartz or fused silica for capillary columns. ... Gas chromatographic separation is always carried out in a column, which is typically "packed" or "capillary". Packed columns ...

*Nadolol

Optimized separation of beta-blockers with multiple chiral centers using capillary electrochromatography-mass spectrometry. J ...

*List of traditional Chinese medicines

ChemEurope.com Optimized separation of pharmacologically active anthraquinones in Rhubarb by capillary electrochromatography, ...

*Electrochromatography

Capillary electrochromatography (CEC) is an electrochromatography technique in which the liquid mobile phase is driven through ... Cikalo, Maria G.; Bartle, Keith D.; Robson, Mark M.; Myers, Peter; Euerby, Melvin R. (1998). "Capillary electrochromatography ... doi:10.1016/0021-9673(94)01276-K. Dittmann, Monika M.; Rozing, Gerard P. (1996). "Capillary electrochromatography - a high- ... It is a combination of high-performance liquid chromatography and capillary electrophoresis. The capillaries is packed with ...

*Capillary electrochromatography

In capillary electrochromatography capillaries, packed with HPLC stationary phase, are subjected to a high voltage. Separation ... "Capillary electrochromatography". The Analyst. 123 (7): 87-102. doi:10.1039/a801148f. ISSN 0003-2654. Smith, N. "Capillary ... Capillary electrochromatography (CEC) combines the principles used in HPLC and CE. The mobile phase is driven across the ... Capillary electrochromatography (CEC) is a chromatographic technique in which the mobile phase is driven through the ...

*User:Plbrown123/Books/Atoms, Ions, Bonds, Molecules, Electronic, and Chemical Analysis; You Don't See that in Dasher! -...

Electrochromatography. Gas chromatography. High-performance liquid chromatography. Capillary electrochromatography. Ion ...

*Dielectrophoresis

Capillary electrochromatography. *Capillary electrophoresis. *Dielectrophoresis. *Difference gel electrophoresis. * ...

*Ion chromatography

Electrochromatography. *Gas chromatography. *High-performance liquid chromatography. *Capillary electrochromatography. *Ion ... "Capillary Ion Chromatography at High Pressure and Temperature". Analytical Chemistry. 84 (16): 7212-7217. doi:10.1021/ac301598j ...

*Affinity chromatography

Electrochromatography. *Gas chromatography. *High-performance liquid chromatography. *Capillary electrochromatography. *Ion ... "Affinity capillary electrophoresis for the assessment of binding affinity of carbohydrate-based cholera toxin inhibitors". ...

*Serum protein electrophoresis

Capillary electrochromatography. *Capillary electrophoresis. *Dielectrophoresis. *Difference gel electrophoresis. * ... Capillary electrophoresis[edit]. In capillary electrophoresis, there is no solid matrix. Proteins are separated primarily by ... The sample is injected into a capillary with a negative surface charge. A high current is applied, and negatively charged ... Depending on whether an agarose or capillary method is used, interferences vary. Immunoglobulins consist of heavy chains (IgA, ...

*Polyacrylamide gel electrophoresis

Capillary electrochromatography. *Capillary electrophoresis. *Dielectrophoresis. *Difference gel electrophoresis. * ...

*Gas chromatography-mass spectrometry

Electrochromatography. *Gas chromatography. *High-performance liquid chromatography. *Capillary electrochromatography. *Ion ... The gas chromatograph utilizes a capillary column which depends on the column's dimensions (length, diameter, film thickness) ...

*Potential applications of carbon nanotubes

By taking advantage of the capillary effects of the small carbon nanotubes, it is possible to condense gases in high density ... Carbon nanotubes have furthermore been grown inside microfluidic channels for chemical analysis, based on electrochromatography ... "Carbon nanotube based separation columns for high electrical field strengths in microchip electrochromatography". Lab on a ...
On-Column Ion-Exchange Preconcentration of Inorganic Anions in Open Tubular Capillary Electrochromatography with Elution Using Transient-Isotachophoretic Gradients. 3. Implementation and Method Development
High-speed capillary electrochromatography was developed on both short and long packed columns with 2 mu m non-porous ODS as the stationary phase. Factors that affect the analysis time of samples, such as voltage, electrolyte concentration, pH and organic modifier concentration in the mobile phase, were studied systematically. Fast analysis of aromatic compounds within 13 seconds was realized with column efficiency of 573,000 plates/m and a R.S.D.% of the retention times of all components in 8 consecutive injections below 1.0%. which demonstrated the high efficiency and high reproducibility of such a technique. In addition, DNPH derived aldehydes and ketones in both standards and environmental samples were separated with high speed ...
article{350485, author = {Lynen, Frederic and BUICA, A and De Villiers, Andr{\e} and CROUCH, A and Sandra, Patrick}, issn = {1615-9306}, journal = {JOURNAL OF SEPARATION SCIENCE}, language = {eng}, number = {13}, pages = {1539--1549}, publisher = {WILEY-V C H VERLAG GMBH}, title = {An efficient slurry packing procedure for the preparation of columns applicable in capillary electrochromatography and capillary electrochromatography-electrospray-mass spectrometry}, volume = {28}, year = {2005 ...
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
Capillary columns packed electrokinetically with 1.5-μm nonporous octadecylsilica particles are used to achieve rapid separations with high efficiencies. A mixture containing five polycyclic aromatic hydrocarbons (PAHs) as test compounds is separated in less than 5 s with 28 kV applied to a column with a packed length of 6.5 cm (10 cm total length). A sample containing 16 PAHs (classified as priority pollutants by the U.S. Environmental Protection Agency) is isocratically separated in under 10 min by using longer columns (15-30 cm). Separation efficiencies greater than 700 000 theoretical plates/m are obtained when detection (via UV-excited laser-induced fluorescence) is performed on the packed portion of the capillary. The outlet frit is observed to play an important role in determining column efficiency. The optimum flow rate for best separation efficiency occurs at ∼2 mm/s. The run-to-run reproducibility of the peak retention times on a single column is better than 1% (relative standard deviation)
Citation: N/A Interpretive Summary: Vitamin E and related compounds are important minor constituents of oilseeds. They provide many health benefits and are useful in oil preservation. State-of-the-art techniques are needed for studying the impact of the minor constituents on the quality and stability of vegetable oils. Separations of the vitamin-E active compounds in edible oils were evaluated by capillary electrochromatography. The new high-speed techniqu requires minimum solvent consumption. The technique can be used by seed growers and the food/oil industries for information on factors affecting oil quality/stability. Food scientists can utilize the optimized distribution data for improving food flavor and quality. Technical Abstract: Separations of lipid antioxidants, tocopherols (T), and tocotrienols (T3) on octylsilica (OS), octadecylsilica (ODS), phenylsilica, or silica were studied by capillary electrochromatography (CEC)-UV detection. The homologues and isomers of the vitamin E-active ...
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
β-Cyclodextrin (β-CD) was anchored onto silica particles at its C(2) position, derivatized primarily at the C(6) position by treatment with bromoacetyl bromide, and finally reacted with two types of cyclams to form cyclam-capped β-CD-bonded silica particles. When used as chiral stationary phases in capillary electrochromatography, these novel bonded silica particles exhibited excellent enantioselectivities for chiral separations. © 2002 Elsevier Science Ltd. All rights reserved ...
Nanoparticles were used as a pseudostationary phase in capillary electrochromatography (CEC) electrospray ionisation-mass spectrometry (ESI-MS) for separation of both neutral analytes by a reversed phase mechanism, as well as for cationic analytes by a cation exchange mechanism. Nanoparticles suspended in electrolyte, were injected as a plug prior to the sample using a partial filling technique (PF), or used as electrolyte in a continuous full filling (CFF) technique. An orthogonal ESI probe was used to hinder the nanoparticles from entering the mass spectrometer and to allow detection of analytes co-eluting with concentrated nanoparticle slurries. Two types of nanoparticles were synthesised and used, both of them having a hydrophobic core and a hydrophilic surface. The hydrophobic core gave the nanoparticles reversed phase properties and the hydrophilic surface promoted the formation of stable slurries of nanoparticles in electrolytes with a low concentration of organic modifier. The surface of ...
The introduction of therapeutic peptides and proteins can be an expensive and time-intensive process. between January 2012 and Dec 2013 publication within the within the two-year period. The parting principles and technical advancements of CE capillary gel electrophoresis capillary isoelectric concentrating capillary electrochromatography and CE-mass spectrometry are talked about alongside exciting brand-new applications of LY2603618 … Continue reading The introduction of therapeutic peptides and proteins can be an expensive. ...
The Journal of Liquid Chromatography and Related Technologies is an internationally acclaimed forum for fast publication of critical, peer reviewed manuscripts dealing with analytical, preparative and process scale liquid chromatography and all of its related technologies, including TLC, capillary electrophoresis, capillary electrochromatography,...
Microcalcifications can be the early and only presenting sign of breast cancer. One shared characteristic of breast cancer is the appearance of mammographic mammary microcalcifications that can routinely be used to detect breast cancer in its initial stages, which is of key importance due to the possibility that early detection allows the application of more conservative therapies for a better patient outcome. The mechanism by which mammary microcalcifications are formed is still largely unknown but breast cancers presenting microcalcifications are more often associated with a poorer prognosis. We combined Capillary Electrochromatography, histology, and gene expression (qRT-PCR) to analyze patient-matched normal breast tissue vs. breast tumor. Potential carcinogenicity of oxalate was tested by its inoculation into mice. All data were subjected to statistical analysis. To study the biological significance of oxalates within the breast tumor microenvironment, we measured oxalate concentration in both
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Fritless particle-loaded monoliths for chiral capillary electrochromatographic (CEC) separation were prepared. Silica particles containing a chiral selector are suspended in a monomer solution, which is drawn into the capillary followed by in situ polymerization. Thereby the silica-based particles containing the chiral selector are embedded in a nonchiral continuous bed. This kind of chiral stationary phase is inexpensive, easy, and reproducible to prepare and circumvents the preparation of frits. As a model, teicoplanin aglycone as chiral selector bonded to 3 μm silica particles was used. The applicability of this approach is demonstrated by means of the chiral separation of aliphatic and aromatic amino acids and dipeptides. As a further application, the chiral selector ristocetin A bonded to 3 μm silica particles was used for the enantiomeric separation of chiral α-hydroxy acids. Since α-hydroxy acids migrate toward the anode, a cationic charge-providing agent was copolymerized with the ...
This contribution relates to the assessment of gradient-elution parameters in capillary liquid chromatography affecting the peak widths in the reversed-phase separation of peptides and intact proteins. Gradient separations were performed using both a poly(sytrene-co-divinylbenzene) monolithic column and a microparticulate fused-core column (silica C18, 2.7 mu m). The applicability of the conventional linear (LSS) and non-linear solvent-strength model (Neue-Kuss) were investigated to describe the retention behaviour of the compounds as a function of the mobile-phase composition. This was performed by using a wide range of gradient conditions, including different gradient slopes (beta, ranging from 0.05 to 0.65 min(-1)) and mobile-phase compositions (Delta phi, i.e. gradient span). Although the LSS-model provided accurate retention time predictions (,1.3% deviation) of scouting runs with more conventional gradient slopes, the prediction of high-speed separations with a high degree of accuracy ...
A microchip laboratory system and method proved fluid manipulations for a variety of applications, including sample injection for microchip chemical separations. The microchip is fabricated using standard photolithographic procedures and chemical wet etching, with the substrate and cover plate joined using direct bonding. Capillary electrophoresis and electrochromatography are performed in channels formed in the substrate. Analytes are loaded into a four-way intersection of channels by electrokinetically pumping the analyte through the intersection, followed by switching of the potentials to force an analyte plug into the separation channel.
The Journal of Chromatography A publishes papers on all aspects of separation science including chromatography, electrochromatography, electrophoresis, hyphenated and other multi-dimensional techniques, sample preparation as well as detection methods such as mass spectrometry. .
An SDRAM memory chip device comprises a non-volatile memory controller for operating a non-volatile memory, e.g., a NAND-flash, and a FIFO memory buffer. The FIFO memory buffer serves to operate background store and load operations between a FIFO buffer array and the non-volatile memory, while a host system such as a CPU exchanges data with the SDRAM work memory. The SDRAM memory chip device, therefore, has at least two additional pins as compared with conventional SDRAM standard for generating a set of additional commands. These commands are employed by the FIFO memory buffer to manage the data transfer between the FIFO buffer and each of the non-volatile memory and the volatile SDRAM memory. Two further pins reflecting the flash memory status provide appropriate issuance of load or store signals by the host system.
An SDRAM memory chip device comprises a non-volatile memory controller for operating a non-volatile memory, e.g., a NAND-flash, and a FIFO memory buffer. The FIFO memory buffer serves to operate background store and load operations between a FIFO buffer array and the non-volatile memory, while a host system such as a CPU exchanges data with the SDRAM work memory. The SDRAM memory chip device, therefore, has at least two additional pins as compared with conventional SDRAM standard for generating a set of additional commands. These commands are employed by the FIFO memory buffer to manage the data transfer between the FIFO buffer and each of the non-volatile memory and the volatile SDRAM memory. Two further pins reflecting the flash memory status provide appropriate issuance of load or store signals by the host system.
A method of preparing a polymer under predetermined temperature conditions comprises conducting the polymerization within a closed reaction chamber configured such that most or all of an inner surface has heat exchange capability. The chambers dimensions are such that the polymerization mixture contacts the effective heat exchange surface sufficiently to ensure that the temperature throughout the polymerization mixture does not vary more than a few degrees from a desired temperature. In one embodiment the chamber is formed of two adjacent, parallel heat exchange plates, wherein at least one plate has a peripheral lip which, in contact with the opposing plate, forms the closed chamber. Multiple plates can be arrayed to form multiple chambers, and each chambers polymerization temperature, as well as resident polymerization mixture, can be individually predetermined. This method and apparatus are particularly useful for preparing polymers used in drag reducing agents for hydrocarbon transportation
Chromatographic column technology in HPLC is an ever-growing field. One area in column technology that has captivated attention in recent years is the use of monolithic columns. Monolithic stationary phases for use in liquid chromatography possess unique characteristics, the most significant of which is a relatively high flow through porosity leading to low column pressure drops, with the potential for use with elevated flow rates leading to faster separations. Silica-based monolithic columns are the most popular phases currently for small molecule separations, although organic polymer-based monoliths have also been studies extensively. Within this thesis the preparation of carbon monoliths of varying flow through porosity for potential chromatographic and electrochemical applications is described. Carbon based monoliths can provide excellent chemical and thermal stability, surpassing those of silica. Unlike alternative materials, carbon monoliths have the advantage of being resistant to ...
How is open-tubular liquid chromatography abbreviated? o-LC stands for open-tubular liquid chromatography. o-LC is defined as open-tubular liquid chromatography rarely.
Rapid chiral separation and impurity determination of ropivacaine and bupivacaine by Densitometry-HPTLC, using mucopolysaccharide as chiral mobile phase additive
download chiral separations for Effective Intervention, 32, 32-39. Our very system uses in its personal day of address. There includes a assigned policy been into the implementation for options to build school-based issues.
CIM® DEAE-8 mL tube is a cylindrical-shaped monolithic column with a column volume of 8 mL. It is encased in a specially designed housing and is
Labscoop is a global science eCommerce engine. Comparison shop millions of products from hundreds of vendors to ensure competitive pricing and reliability. Chiral Compounds Biochemicals & Reagents
The resistance to the mass transfer term for the stationary phase must be considered in isolation. The experimentally observed plate height (variance per unit length) resulting from a particular dispersion process [e.g., (hs), the resistance to mass transfer in the stationary phase] will be the sum of the local plate height contributions (h); i.e.,. ...
A method for separating plasma from red blood cells and a device utilizing the method in which a low-pressure filter is interposed in a pathway between an inlet port and a reaction area. The sole driving force for the movement of plasma from the filter to the reaction area is capillary force provided by a tubular capillary. The filter is selected from glass microfiber filters of specified characteristics, which can operate in the absence of agglutinins, and filters capable of separating agglutinated red cells from a plasma, which require the use of an agglutinin.
The present invention provides an endoprosthesis assembly having a reduced cross sectional profile for percutaneous deployment and implantation within a body passageway such as a blood vessel. The assembly comprises a radially expandable cylindrical frame having first and second ends, and a first unexpanded outer diameter and a second expanded outer diameter. The assembly further comprises a radially expandable elastomeric sleeve surrounding a length of the frame and having first and second ends and a first unexpanded inner diameter and a second expanded inner diameter. The inner diameter of the expanded sleeve is not greater than the outer diameter of the expanded frame. The second expanded inner diameter of the sleeve is in a range from about 60% to about 380% greater than the first unexpanded inner diameter of the sleeve. The assembly of the preferred embodiment is adapted to be deployed through an introducer smaller than 9 French and the sleeve is capable of expanding to an inner diameter of about
For 60 years, Regis Technologies, Inc. has partnered with pharmaceutical and biotechnology companies to help expedite drugs to market. ...
A global strategy for the analysis of glycoproteins by capillary electrophoresis and capillary liquid chromatography coupled to mass spectrometry. Application to quality control of biopharmaceuticals and disease diagnosis ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Putting it here so I remember to read more about it later, but 90 second automated digestions!??! They average 60% coverage of proteins at small as cytochrome C and as big as HSA (~67kDa ...
SPE Part: 8E-S038-TGB Strata™-X-AW 33 µm Polymeric Weak Anion, 30 mg / well, 96-Well Plates , 2/Pk Phase: Polymeric weak anion exchange Sorbent Type: Polymer-based Format: 96-Well Plate Target Analytes: Acidic compounds (i.e.) strong acids
The COSMOSIL 5C18-AR-II packed column features a polymeric type of C18 reversed phase material. The acidic resistance of COSMOSIL 5C18-AR-II is much improved compared with commercially available monomeric type octadecyl stationary phases. It retains high performance even in case of acidic mobile phases commonly used to separate acidic compounds and peptides.. Figure ...
... phases are the workhorse phases for preparative and process scale SFC purifications. Celeris 2EP (2-ethyl pyridine) and 4EP (4-ethyl pyridine) media is designed to be highly reproducible based on tight product specifications and low metal content. Celeris 2EP is especially well-suited for separation of acidic compounds and exhibits broad selectivity towards a variety of other compound types. The Celeris 4EP phase offers alternate selectivity to the 2EP phase. Celeris 2EP and 4EP SFC columns deliver high performance separations comparable to other ethyl pyridine phases at a much lower price ...
SPE Part: 8B-S009-KDG Strata® NH2 (55 µm, 70 Å), 2 g / 12 mL, Giga Tubes , 20/Pk Phase: NH2 Sorbent Type: Silica-based Format: Tube Target Analytes: Acidic compounds (i.e.) strong acids
Negative mode (applies a negative voltage to the source): acidic compounds as the acid loses a proton (general buffers: ammonium, triethylamine ...
UCTs Enviro-Clean® anion exchange sorbents provide unmatched activity for the extraction of acidic compounds. The analyst can choose from a wide range of cartridge sizes, sorbent types and quantities tailored to the specific requirements of each analysis.
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My research focuses on optimising the analysis of protein species by ESI compatible surface sampling techniques such as LESA (Advion) and the flowprobe (Prosolia). Optimisation of extraction, separation and then structural characterisation of protein (and other biologically relevant species) from biological substrates such as dried blood spots and thin tissue sections is a particular focus. Parameters such as extraction solvent and sampling protocol have been optimised by each sampling procedure. Separation of molecular species extracted from biological samples has been achieved by coupling to an ultra FAIMS chip device (Owlstone, UK). Detected species have been identified via tandem MS/MS experiments e.g. CID, HCD and ETD. Data has been acquired using the following instrumentation: LTQ Orbitrap Velos/Elite (Thermo Fisher Scientific), Synapt G2S (Waters).. ...
Monolith chemistry can be customized to perform multiple sequential process steps. This reduces per-sample labor costs and mitigates the risk of human error.. The fundamental monolith formulations are bio-inert, and can be configured to perform a variety of filtration operations. In addition, monolith can be modified to perform ion-exchange, DNA hybridization, fluorescent probe immobilization and even enzymatic reactions.. ...
Read reviews and compare manufacturers of Supercritical Fluid Chromatography > Columns > Open Tubular Capillaries products in the SelectScience products and suppliers directory
Capillary electrophoresis (CE) comprises a family of related separation techniques in which an electric field is used to achieve the separation of components in a mixture. One of the key features of CE is the simplicity of the instrumentation required, and today, these techniques have become powerful analytical tools for high efficiency separation, and the identification of a variety of ionic and neutral compounds.. Moreover, the versatility of CE operational modes (capillary zone electrophoresis, micellar electrokinetic capillary chromatography, capillary electrochromatography, capillary isoelectric focusing, isotachophoresis, non-aqueous capillary electrophoresis, etc.) and detection systems (ultraviolet, fluorescence, amperometric, mass spectrometry…) make CE useful for applications in many fields such as environmental, food, pharmaceutical, and forensic analysis. The typcial drawback of CE techniques, based on their low sensitivity when dealing with conventional UV-detection, due to their ...
Studies of the capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MEKC) behaviour of 1,4-benzodiazepines have seen application in subject areas such as the development of pharmaceuticals, therapeutic drug monitoring and forensic toxicology. In the development of pharmaceuticals, pK(a) determinations by CZE can be used in preclinical studies whereas analytical data on the detection and determination of 1,4-benzodiazepines is of value primarily in raw material/formulation assay and in the analysis of body fluids in clinical studies. The capillary electrophoresis (CE) techniques, which generally have inferior limits of detection (LOD) to rival techniques such as gas chromatography (GC) and high-performance liquid chromatography (HPLC), are particularly applicable in forensic toxicology where reasonably high concentrations of these drugs can be encountered. It is anticipated that, with the interfacing of CZE: and capillary electrochromatography (CEC) with mass ...
A colloidal crystal is an ordered array of colloid particles, analogous to a standard crystal whose repeating subunits are atoms or molecules. A natural example of this phenomenon can be found in the gem opal, where spheres of silica assume a close-packed locally periodic structure under moderate compression. Bulk properties of a colloidal crystal depend on composition, particle size, packing arrangement, and degree of regularity. Applications include photonics, materials processing, and the study of self-assembly and phase transitions. IUPAC definition Assembly of colloid particles with a periodic structure that conforms to symmetries familiar from molecular or atomic crystals. Note: Colloidal crystals may be formed in a liquid medium or during drying of particle suspension. A colloidal crystal is a highly ordered array of particles which can be formed over a long range (to about a centimeter). Arrays such as this appear to be analogous to their atomic or molecular counterparts with proper ...
Chiral separations of five β-adrenergic antagonists (propranolol, esmolol, atenolol, metoprolol, and bisoprolol) were studied by capillary electrophoresis using six cyclodextrins (CDs) as the chiral selectors. Carboxymethylated-β-cyclodextrin (CM-β-CD) exhibited a higher enantioselectivity power compared to the other tested CDs. The influences of the concentration of CM-β-CD, buffer pH, buffer concentration, temperature, and applied voltage were investigated. The good chiral separation of five β-adrenergic antagonists was achieved using 50 mM Tris buffer at pH 4.0 containing 8 mM CM-β-CD with an applied voltage of 24 kV at 20 °C. In order to understand possible chiral recognition mechanisms of these racemates with CM-β-CD, host-guest binding procedures of CM-β-CD and these racemates were studied using the molecular docking software Autodock. The binding free energy was calculated using the Autodock semi-empirical binding free energy function. The results showed that the phenyl or naphthyl ring
TY - JOUR. T1 - Development of an affinity silica monolith containing α1-acid glycoprotein for chiral separations. AU - Mallik, Rangan. AU - Xuan, Hai. AU - Hage, David S.. PY - 2007/5/18. Y1 - 2007/5/18. N2 - An affinity monolith based on silica and containing immobilized α1-acid glycoprotein (AGP) was developed and evaluated in terms of its binding, efficiency and selectivity in chiral separations. The results were compared with data obtained for the same protein when used as a chiral stationary phase with HPLC-grade silica particles or monoliths based on a copolymer of glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA). The surface coverage of AGP in the silica monolith was 18% higher than that obtained with silica particles and 61% higher than that measured for a GMA/EDMA monolith. The higher surface area of the silica monolith gave materials that contained 1.5- to 3.6-times more immobilized protein per unit volume when compared to silica particles or a GMA/EDMA monolith. The ...
Abstract: Many pharmaceuticals contain active ingredients that have more than one stereoisomer. An important concern is the recognition that these different stereoisomers do not necessarily have identical, or even desirable biological activity. Consequently, analytical methods for the analysis and separation of enantiomers are important in the proper development of a marketed pharmaceutical product. In this research, direct HPLC methods for the chromatographic separation of oxyphene optical isomers have been developed and optimized using three types of chiral stationary phases. The research carried out a systematic study of the conditions for the separation of oxyphene optical isomers using synthetic polymer chiral stationary phase of cellulose tris (3, 5-dimethylphenylcarbamate) Chiralcel OD, ß-cyclodextrin chiral stationary phase, and a1-acid glycoprotein chiral stationary phase. The methods using the ß-cyclodextrin and Chiralcel OD columns provide for the accurate determination of the ...
Rapid determination of theaflavins by HPLC with a new monolithic column | Jianyong Zhang, Hongchun Cui, Heyuan Jiang, Lei Fang, Weiwei Wang, Wei Su, Chunhua Xiong | Agricultural Journals
Papaverine hydrochloride Spasm of smooth muscles: abdominal organs (cholecystitis, pilorospazm, spastic colitis, renal colic), peripheral vascular (occlusive disease), cerebrovascular, cardiac - angina (in the complex therapy), bronchospasm; as an auxiliary drug for sedation.
The effects of some factors important in ion-pair, high-performance liquid chromatography were studied in ion-pair, thin-layer chromatography. Tetramethyl and cetyltrimethylammonium salts were used...
The degree of acidity or alkalinity is an important property of our blood. The acidity level increases with the increase in the level of acidic compounds in our body, or when the level of basic (also known as alkaline) compounds present in our body falls. The alkalinity level in our body increases when the reverse takes place, i.e., when the level of acidic compounds falls or when the basic compounds in the body increase. The acidity or alkalinity of any solution is indicated on the pH scale. Even a minor shift from the normal range can destroy our organs. Generally, the normal acidic level (pH) of our blood is close to 7.4. When the pH level falls below 7.35, then we say that acidosis has occurred. On the other hand, when the pH goes above 7.45, then we say that alkalosis has occurred. We have a severe case of the former, when the pH value of blood goes below 7.0; while a severe case of the latter can occur when the pH value goes above 7.5. Let us now see what are the various causes and ...
In this work, a new oligomer matrix-assisted dispersion (OMAD) method for the preparation of homogeneous dispersion of multi-walled carbon nanotubes (MWNTs) incorporated monolithic column was developed. Oligomers matrix as a scaffold could allow MWNTs to entangle with it instead of self-aggregation, so the MWNTs remain in the polymer network followed by in situ self-solidification. The OMAD method not only greatly enlarged the BET surface area of MWNTs incorporated monolithic column from 13.8 m(2) g(-1) to 85.5 m(2) g(-1) without a significant effect on the surface chemistry of the MWNTs, but also improved the dispersion of MWNTs making its content up to 5 wt% (with respect to monomers). The synthesized materials combine the favorable attributes of both high permeability and large surface area, making them excellent candidates for on-line separation and enrichment of proteins. The oligomer matrix-assisted dispersion MWNTs incorporated monolithic columns (OMAD-MMC) exhibited higher enrichment ...
A team from the physics department of the Johannes Gutenberg University, Mainz, has investigated nucleation processes and interfacial properties of colloidal crystals. Nucleation is omnipresent in our daily life and describes events as diverse as the formation of rain in clouds, the crystallization of proteins or the growth of nano-particles. The studies undertaken using supercomputers Hazel Hen and Hornet of HLRS Stuttgart contribute towards a more fundamental understanding of these processes and the underlying theoretical foundation.
Researchers have developed and tested a prototype able to provide image data at 60 frames per second, and plan next to conduct animal studies that could lead to commercialization of the device.. "Our device will allow doctors to see the whole volume that is in front of them within a blood vessel," said F. Levent Degertekin, a professor in the George W. Woodruff School of Mechanical Engineering at the Georgia Institute of Technology. "This will give cardiologists the equivalent of a flashlight so they can see blockages ahead of them in occluded arteries. It has the potential for reducing the amount of surgery that must be done to clear these vessels.". Details of the research were published online in the February 2014 issue of the journal IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control. Research leading to the device development was supported by the National Institute of Biomedical Imaging and Bioengineering (NIBIB), part of the National Institutes of Health.. "If youre a ...
Principal Investigator:OKAMOTO Yoshio, Project Period (FY):1998 - 2000, Research Category:Grant-in-Aid for Scientific Research on Priority Areas (B)
Meropenem is a widely used antimicrobial for the treatment of infections associated with the use of invasive medical devices in intensive care unit patients. These treatments are not always effective, in fact, in-vitro studies have demonstrated the difficulty of antimicrobials to penetrate into the biofilm, however in-vivo studies of the effect of these compounds is a trend, mostly because of the complexity of pulmonary samples extracted from ETTs. Therefore, the objective of this study was to evaluate in-tube solid phase microextraction (in-tube SPME) coupled to capillary liquid chromatography (CapLC) with DAD to determine meropenem in ETTs in order to estimate the penetration capability into the biofilm ...
Preparative Supercritical Fluid Chromatography (pSFC). As an environment-friendly approach to purification applications, pSFC is being used routinely for an increasing number of applications.. During the recent years SFC has gained acceptance in drug discovery for high-throughput purification of enantiomers. Hence, pSFC replaces preparative HPLC (pHPLC) as major technology for chiral separations in many pharmaceutical companies. Preparative SFC excels not only at separating and purifying chiral compounds but is also powerful for achiral separations and can be implemented in most steps of drug discovery. It may offer potential advantages over pHPLC for purity upgrading of active pharmaceutical ingredients or drug intermediates, impurity isolation and non-aqueous purification.. The use of pSFC as an alternative option to pHPLC receives also a growing interest for natural products, environmental (pesticides) and petroleum (biofuels) applications. ...
Preparative Supercritical Fluid Chromatography (pSFC). As an environment-friendly approach to purification applications, pSFC is being used routinely for an increasing number of applications.. During the recent years SFC has gained acceptance in drug discovery for high-throughput purification of enantiomers. Hence, pSFC replaces preparative HPLC (pHPLC) as major technology for chiral separations in many pharmaceutical companies. Preparative SFC excels not only at separating and purifying chiral compounds but is also powerful for achiral separations and can be implemented in most steps of drug discovery. It may offer potential advantages over pHPLC for purity upgrading of active pharmaceutical ingredients or drug intermediates, impurity isolation and non-aqueous purification.. The use of pSFC as an alternative option to pHPLC receives also a growing interest for natural products, environmental (pesticides) and petroleum (biofuels) applications. ...
D-glucopyranoside, also known as hexose or D-galactose, is a member of the class of compounds known as hexoses. Hexoses are monosaccharides in which the sugar unit is a is a six-carbon containing moeity. D-glucopyranoside is soluble (in water) and a very weakly acidic compound (based on its pKa). D-glucopyranoside can be found in a number of food items such as marzipan, olive, channel catfish, and rubus (blackberry, raspberry), which makes D-glucopyranoside a potential biomarker for the consumption of these food products. D-glucopyranoside exists in all living organisms, ranging from bacteria to humans. The name was originally given to plant products of this nature, in which the other part of the molecule was, in the greater number of cases, an aromatic aldehydic or phenolic compound (exceptions are sinigrin and jalapin or scammonin). It has now been extended to include synthetic ethers, such as those obtained by acting on alcoholic glucose solutions with hydrochloric acid, and also the ...
The Chiral Chromatography Learning Center provides technical resources to help you choose a chiral HPLC or GC column, troubleshoot a chiral method, or enhance your understanding of chiral separations.
These kits provide the necessary columns to perform most chiral separations and run mechanistic studies, and are offered at very attractive prices.
Supelco DEX Kit I is valuable for studying the effect of CD size on a separation. All three columns in the kit, α-DEX 120, β-DEX 120, and γ-DEX 120, have the same permethylated derivative, but on different size CDs. One application is to confirm enantiomer identification by monitoring elution order changes (enantioreversal) from one column to another.
Supelco DEX Kit II contains four columns, each 30 m x 0.25 mm I.D., 0.25 μm, one each of β-DEX 120, β-DEX 225, γ-DEX 225, and β-DEX 325. Combining this kit with Supelco DEX Kit I spans the full range of Supelco DEX column enantioselectivity.
Previously determined retention data for a series of benzodiazepine (BDZ) derivatives, comprising nine achiral compounds, four single enantiomers, and 18 individual isomers of nine racemates, on a chiral stationary phase based on immobilized human serum albumin (HSA) were analyzed to define quantitative relationships between structure and enantiospecific retention. Structural parametrization of the agents was done by means of hydrophobic fragmental constants and electronic and steric parameters obtained by computational chemistry methods. A structural descriptor was identified, a submolecular measure of polarity about the stereogenic center, that accounted for the stronger electrostatic interactions of the second-eluting enantiomer with the HSA chiral stationary phase. Quantitative structure-enantiospecific retention relationships were derived for both enantiomeric series and for achiral compounds, and structural requirements for binding to HSA were determined. Two types of binding sites were ...
Monoliths," the latest track from Deerhunter guitarist Lockett Pundts solo project Lotus Plaza, is a lush, summery anthem. With layers of radiant guitars and a high-flying chorus, it conjures the wide-open spaces of a road trip through the plains states, or rope-swinging into an icy lake on a hot afternoon. Lotus Plazas second full-length, Spooky Action At A Distance, is out tomorrow on Kranky.. Stream: Lotus Plaza, "Monoliths" ...
Flame Sampling Probe Assembly for PerkinElmer AS-90/90A/90 Plus/91/93 Plus/S10 Autosampler from PerkinElmer. A Sampling Probe Assembly Component.
Intempco Digital RTD thermometer LCD LED temperature gauge display indicator transmitter sensor probe sanitary PT100 PT1000 Nema4X enclosure battery powered 4-20mA output replace Bi-Metal Dial capillary liquid bulb glass mercury M12
Morton Grove, IL (PRWEB) June 01, 2015 -- Regis Technologies, Inc. is a recognized leader in developing and manufacturing of chiral stationary phases (CSPs)
Another day, another monolith Ruby gem to reach deep into the bowels of your app config, models, views, controllers and make you manage uploadin...
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Buy or Rent Chiral Separations by Capillary Electrophoresis as an eTextbook and get instant access. With VitalSource, you can save up to 80% compared to print.
Chiral separation of enantiomers is one of the most challenging tasks for any analytical technique including capillary electrophoresis (CE). Since the first report in 1985 showing the great possibilities of CE for the separation of chiral compounds, the amount of publications concerning this topic has quickly increased. Although chiral electromigration methods have mainly been used for enantioseparation of drugs and pharmaceuticals, they have also been applied to analyze chiral pollutants. This article intends to provide an updated overview, including works published till January 2005, on the principal applications of CE to the chiral analysis of pollutants and their metabolites, with especial emphasis on articles published in the last ten years. The main advantages and drawbacks regarding the use of CE for chiral separation of pollutants are addressed including some discussion on the foreseen trends of electromigration procedures applied to chiral analysis of contaminants ...
Chernobyl. Three Mile Island. Fukushima. Accidents at nuclear power plants can potentially cause massive destruction and expose workers and civilians to dangerous levels of radiation that lead to cancerous ...
Carvedilol is administered as a racemic mixture of the R(+)- and S(-)-enantiomers, although it was demonstrated that the two enantiomers exhibit different pharmacological effects and stereoselective pharmacokinetics. The aim of this study was the evaluation of several native and derivatized cyclodextrines as chiral selectors for the separation of carvedilol enantiomers. Stereoselective interactions were observed with four cyclodextrines (β-CD, hydroxypropyl-β-CD, randomly methylated β-CD and sulfobuthyl ether- β-CD). The effects of CD concentration, pH value and composition of the background electrolyte, capillary temperature, running voltage and injection parameters have been investigated. The method was validated for precision of peak-area response, linearity range and limits of detection and quantification. An efficient stereoselective capillary zone electrophoretic method was developed for the determination of carvedilol enantiomers using a simple 25 mM phosphate buffer at a pH = 2.5 and 10 mM
The extent of exothermicity associated with the construction of large-volume methacrylate monolithic columns has somewhat obstructed the realisation of large-scale rapid biomolecule purification especially for plasmid-based products which have proven to herald future trends in biotechnology. A novel synthesis technique via a heat expulsion mechanism was employed to prepare a 40 mL methacrylate monolith with a homogeneous radial pore structure along its thickness. Radial temperature gradient was recorded to be only 1.8 °C. Maximum radial temperature recorded at the centre of the monolith was 62.3 °C, which was only 2.3 °C higher than the actual polymerisation temperature. Pore characterisation of the monolithic polymer showed unimodal pore size distributions at different radial positions with an identical modal pore size of 400 nm. Chromatographic characterisation of the polymer after functionalisation with amino groups displayed a persistent dynamic binding capacity of 15.5 mg of plasmid ...
Quantification of cannabinoids in cannabis plant material and cannabis based products is performed by High Pressure Liquid Chromatography (HPLC). The choice of HPLC is related to the superiority of HPLC as an analytical tool for determination of components in herbal medicines. As opposed to GC, the main alternative analytical method, HPLC directly detect acidic compounds, allowing their accurate quantification. This hold special significance in cannabis as the natural predominant form of cannabinoids is the acidic parental compound. Therefore, HPLC enables characterization and quantification of cannabinoids in their natural form. Furthermore, our method validation is performed under USP guidelines.. ...
Simple. When you put lemon juice on a onion, it no longer tears your eyes. (Dont know exactly how, perhaps something to do with the acidity of the lemon) Well heres the idea. Canned, sprayable lemon juice to spray on onions while cutting them? Perhaps different flavoured versions of it (Extract the acidic compound and use it alone and mix it with flavour). Lemon, garlic, bubble gum, whatever. Would also prove as an alternative method to eating fish and chips, and/or veal ...
Elite-MTBE, Low Polarity Stationary Phase, Length: 30m, Inner Diameter: 0.53mm, Film Thickness: 3.00mm, Temperature Limits: 10 to 250°C
Elite-XLB, Specialty Phase for GC/MS with Exceptionally Low Bleed, Length: 15m, Inner Diameter: 0.32mm, Film Thickness: 0.25mm, Temperature Limits: 30 to 340/360°C
Elite-XLB, Specialty Phase for GC/MS with Exceptionally Low Bleed, Length: 30m, Inner Diameter: 0.32mm, Film Thickness: 1.00mm, Temperature Limits: 30 to 340/360°C
BCCs goal in conducting this study is to provide an overview of the current and future global market for chiral products and technologies used in todays world. The key objective is to present a comprehensive analysis of the current market and its future direction in the chiral market as an important tool in various therapeutic industries and other industries. This report explores present and future strategies within the chiral market, including the synthesis, separation and analysis of chiral products. The improvisation of the market, the setbacks and the needs of the market are discussed in this report. An overview of chirality, and the chiral products and technologies used in the past and currently are discussed in this report. A detailed analysis of the structure of the chiral industry has been conducted. This includes chiral synthesis, chiral separation/resolution and chiral analysis. Revenues are broken down by global region from 2009 to 2011 and estimated for the five-year period from ...
This distribution system is not dominated by molecular forces. The relatively large entropy change is a measure of the loss of randomness or freedom that happens when the solute molecule transfers from one phase to the other. The more random and more free the solute molecule is in a particular environment, the greater its entropy in that environment. The large entropy change shown in system (B) (figure 2), indicates that the solute molecules are more constrained in the stationary phase (e.g., confined in the pores of the exclusion media) than they were in the mobile phase. This restriction is responsible for the greater distribution of the solute in the stationary phase and its greater retention. Because the change in entropy is the major contribution to the change in standard energy,. In thermodynamic terms, the distribution is entropically driven. Chiral separations and separations that are dominated by size exclusion are examples of entropically driven separations. It is important to ...
1. A method of manufacturing a plasma display panel including:a step of disposing a front panel and a back panel opposed to each other having a communication hole to inside the opposed front and back panels with a sealing material for forming a discharge space inside;a step of disposing the discharge space between a pedestal and the back panel, the pedestal having a hole area larger than an area of the communication hole and an inner diameter area of an exhaust pipe and having a gas absorbing material of which an outer diameter is smaller than an inner diameter of the pedestal and greater than the inner diameter of the exhaust pipe, positioning the exhaust pipe having the pedestal with an exhaust pipe fixing material so that the pedestal surrounds the communication hole of the back panel; after that,a step of sealing the front panel and the back panel opposed to each other and the exhaust pipe, hardening the sealing material and the exhaust pipe fixing material by cooling, after softening the ...
Jean Blehaut holds a MS degree in Chemical Engineering from ENSIC (Ecole Nationale Supérieure des Industries Chimiques in Nancy, France) and a MS degree in Biochemistry from INSA (Institut National des Sciences Appliquées in Lyon, France).. Jean first started in R&D and was involved in the development of a purification process for omega-3 polyunsaturated fatty acids. In 1995, he became part of the founding team of Novasep. He was responsible for the development of SMB technology at Novasep and succeeded in developing the first ton-scale chiral separation by SMB in the pharmaceutical industry. Between 1999 and 2004, he held the position as CEO of Novasep, Inc. in Boothwyn, PA, USA, in charge of North American business. From 2004 to 2008, he was President of the Pharma Business Unit of Novasep Process (a division of Novasep in Pompey, France). He currently holds the position as Corporate Director of Innovation and Marketing at Novasep.. Mr. Blehaut is author or co-author of several patents and ...
Considerable progress has been made in the development of synthetic CSPs for the direct chromatographic separation of enantiomers. An innate advantage of synthetic CSPs is the ability to prepare either optical antipode of the CSP as well as the racemic analogs. CSPs derived from the 3,5-dinitrobenzamides of $\alpha$-amino acids have been prepared and extensively evaluated for the separation of enantiomers. We became interested in the effect on enantiomer separations of a second stereogenic center in the CSP as a result of our studies of CSPs which have a single stereogenic center. To this end, a new $N$-(3,5-dinitrobenzoyl)-$\beta$-amino acid derived CSP was synthesized and evaluated for the separation of enantiomers ...
Porous layer open tubular monolith capillary column: switching-off the reaction kinetics as the governing factor in their preparation by using an immiscible liquid-controlled polymerization
The Departmental Chromatography Service offers access to both gas and liquid chromatography. An extensive suit of GCs is available with a selection of columns that allow for analysis of a variety of volatile analytes including the separation of chiral compounds.. Currently four systems are available to carry out liquid chromatography, two of these systems are also set up for preparative HPLC. The available detectors on these instruments are variable fixed wavelength, diode array, light scattering and mass spectrometry coupled with several different phased columns allows for the separation of most compounds from small molecules to small proteins including some chiral enantiomers.. All of the instruments go through routine maintenance and undergo regular service by an external body. This ensures that reproducibility is constantly maintained. Users can be assured of high quality results.. The service can be used in two ways. The first being to submit samples alongside a method for analysis by one ...
BACKGROUND:. Client requested synthesis of 9 kg with 98% purity and 98% e.e. of a novel chiral compound under GLP. CHALLENGES:. Clients route was 12 steps requiring preparative HPLC and Chiral SPC separation, requiring large amounts of m-CPBA, NaN3, and LAH. Complexity and reagent cost made it not suitable for scale-up. ...
Overview Today is a 51 minute lecture on chiral compound properties. Details Optical isomers show optical activity or rotate plane polarized light. Polarized sunglasses demonstrate this property. Single enatiamers are generally optically active. How to determine the optical purity? Find the angle to which the plane polarized light rotates about. a = [a] c l…
A method and apparatus for measuring the flow rate of a fluid within tubing disposed within a downhole wellbore. The present invention provides an inverse Venturi meter inserted within tubing with an enlarged inner diameter portion. Pressure differential is measured between the enlarged inner diameter portion and another portion of the inverse Venturi meter. Flow rate is determined from the pressure differential and the density of the fluid.
... This first fritless SPE product range provides greater reproducibility with cleaner, more consistent extracts., SOLA products provide un
Page contains details about vinyltrimethoxysilane-modified cellulose nanofiber micro-honeycomb monoliths . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Browse Chromatography, Column, Reservoir in the NDS Technologies, Inc. catalog including Item #,Product Name,Nominal Volume,Reservoir Capacity,Column Inner Diameter,Column Length,List Price
The SKF U-joint seal has several design advantages and makes it possible to reduce maintenance cost. This maintenance free bearing and appropriate snap rings, where SKF produces both bearing cups and crosses to guarantee fitting tolerances between the needle rollers and the inner diameter of the cup. SKF grinds both the inner and outer surfaces of the cups ...
FIAS Sampling Probe Assembly for PerkinElmer AS-90/90A/90 Plus/91/93 Plus Autosampler from PerkinElmer. 1.0mm Inner Diameter. A Sampling Probe Assembly.
As if Glassjaw just dropped a new track out of the blue. Heres New White Extremity from their as-yet undetailed third album.
1. Morphine sulphate in high concentrations when added to the pace-maker causes increased tonus and "pendular movements" of the exposed ureter.. 2. In moderate concentrations when added to the pace-maker morphine causes increased tonus and peristaltic activity if peristalsis exists and antiperistalsis if antiperistalsis is present.. 3. If morphine sulphate is added to the solution bathing the dependent segment of ureter a reverse movement is quickly established, peristalsis is converted into antiperistalsis and vice versa.. 4. Papaverine hydrochloride depresses both peristalsis and antiperistalsis in the ureter.. 5. Papaverine hydrochloride added to the solution bathing the pace-maker quickly stops the spontaneous activity of that segment and in irritable ureters reverses the form of activity thus if peristalsis was present antiperistalsis is produced and vice versa.. 6. Papaverine hydrochloride decreases the rate and height of contraction.. 7. It requires about one-hundredth the quantity of ...
We have combined high-performance liquid chromatography (HPLC) separations using a monolithic column with acidic potassium permanganate and tris(2,2-bipyridyl)ruthenium(II) chemiluminescence detection in a rapid and highly sensitive method to monitor the process of extracting opiate alkaloids from Papaver somniferum. Due to the high flow rates allowed with the monolithic column and the inherent selectivity of the chemiluminescence reactions, the four predominant alkaloids - morphine, codeine, oripavine and thebaine - were determined in less than 2 min. The results obtained with numerous process samples compared favourable with those of the standard HPLC methodology. Limits of detection were 11010 M, 51010 M, 51010M and 1109 M, for morphine, codeine, oripavine and thebaine, respectively.. ...
A cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) technique has been developed for enantioseparation of vinpocetine using an inexpensive 2-hydroxypropyl-β-CD (HP-β-CD) as the chiral selector (CS). The best chiral separation was achieved using 40 mM HP-β-CD as the CS in 50 mM phosphate buffer (pH 7.0) consisting of 40 mM sodium dodecyl sulfate (SDS) at a separation temperature and separation voltage of 25°C and 25 kV, respectively. To the authors best knowledge, this is the first CD-MEKC study able to successfully separate the four stereoisomer of vinpocetine in separation time of 9.5 min and resolution of 1.04-3.87. Chirality, 2012. © 2012 Wiley Periodicals, Inc. ...
The mechanism by which chiral selectivity takes place is complicated by the surface morphology, the possible involvement of the solvent, and the characteristics of the chiral molecules at the surface. My goal is to model and understand the factors which lead to significant discrimination in the case of three closely related chiral stationary phases: N-(1-phenylethyl)-N-[3-(triethoxysilyl)propyl]-urea (PEPU), [(3,5-dinitrobenzoyl)-amino]-N-[3-(triethoxysilyl)propyl]-2-phenylacetamide (DNB-phenyglycine), and [(3,5-dinitrobenzoyl)amino]-N-[3-(triethoxysilyl)propyl]-4-methylpentanamide (DNB-leucine). Ab initio calculations are used to develop molecular models of these chiral selectors. These models are employed in molecular dynamics (MD) simulations, which provide the theoretical framework for modelling chiral interfaces in different solvent mixtures. The MD simulations of PEPU interfaces show that, in alcohol/water mixtures, the alcohols form domains at the interface with the hydrophobic portions ...
Purpose: : Development of clinically useful biomarkers for glaucoma is an area of active investigation, and proteomics is at the center of these activities. This proteomic study aimed to comparatively identify the presence and abundance of proteins in serum samples of patients with different subgroups of glaucoma and non-glaucomatous controls. Methods: : Serum samples were collected from 196 patients with glaucoma, including primary open-angle glaucoma (POAG) and pseudoexfoliative glaucoma (XFG), and an age-matched control group of 92 healthy volunteers without glaucoma or any other ocular disease. Un-depleted serum samples were quantitatively analyzed in 5 random samples from each group by two-dimensional capillary liquid chromatography and linear ion trap mass spectrometry (LC-MS/MS). Scaffold Proteome Software was used to validate MS/MS-based peptide and protein identifications. Peptide identifications were accepted if they could be established at ,95.0% probability as specified by the ...
The research presented in this dissertation involves the synthesis, modification, characterization, and the application of polymeric surfactants in micellar electrokinetic chromatography (MEKC). Sodium undecenoyl-L-Leucinate (L-SUL) was synthesized and modified by use of alkali metal counterions, alcohols, and Triton X-102. Characterization of the modified poly-L-SUL was performed in order to elucidate the structural changes on the surfactants by use of four techniques. While surface tensiometry was used for the determination of the critical micelle concentration (cmc), fluorescence measurements were used for the determination of the polarity of the modified polymeric surfactants. The information about the size of the polymeric surfactants was obtained by use of pulsed field gradient-NMR (PFG-NMR). A superior chromatographic performance in MEKC was demonstrated by the modified polymeric surfactants as compared to the non-modified polymeric surfactants. A correlation between the polarity and the ...
Separating a chiral drug into its single beneficial enantiomer reduces the risk of adverse effects from the hazardous enantiomer, which is inactive. Rapid development of chiral separation technology in recent years led the process chemists to choose different methodologies to separate or synthesis optically active chiral chemicals. These methodologies have major applications in manufacturing pharmaceutical drugs, agrochemicals, food sweetening agents, and aromatic compounds for cosmetic industries. In many methodologies, asymmetric synthesis is predicted to be the most preferred and it is anticipated to grow its utilization over the forecast period.. Some of the prominent factors that positively influence the growth of Chiral Chemicals Market are increasing pharmaceutical drugs manufacture owing to global demand for medicines is growing, as demographic, economic and epidemiological trends reshape the marketplace, rising demand for to make sweetening food additives optically active and effective ...

An efficient slurry packing procedure for the preparation of columns applicable in capillary electrochromatography and...An efficient slurry packing procedure for the preparation of columns applicable in capillary electrochromatography and...

... procedure for the preparation of columns applicable in capillary electrochromatography and capillary electrochromatography- ... procedure for the preparation of columns applicable in capillary electrochromatography and capillary electrochromatography- ... Procedure for the Preparation of Columns Applicable in Capillary Electrochromatography and Capillary Electrochromatography- ... procedure for the preparation of columns applicable in capillary electrochromatography and capillary electrochromatography- ...
more infohttps://biblio.ugent.be/publication/350485

Synthesis of cyclam-capped β-cyclodextrin-bonded silica particles for use as chiral stationary phases in capillary...Synthesis of cyclam-capped β-cyclodextrin-bonded silica particles for use as chiral stationary phases in capillary...

Capillary electrochromatography. Chiral stationary phase. Crown ether. Cyclam. Enantiomeric separation. Issue Date: 25-Mar-2002 ... When used as chiral stationary phases in capillary electrochromatography, these novel bonded silica particles exhibited ... of cyclam-capped β-cyclodextrin-bonded silica particles for use as chiral stationary phases in capillary electrochromatography ... of cyclam-capped β-cyclodextrin-bonded silica particles for use as chiral stationary phases in capillary electrochromatography ...
more infohttp://scholarbank.nus.edu.sg/handle/10635/77177

Advances in Capillary Electrochromatography: Rapid and High-Efficiency Separations of PAHs - Semantic ScholarAdvances in Capillary Electrochromatography: Rapid and High-Efficiency Separations of PAHs - Semantic Scholar

... is performed on the packed portion of the capillary. The outlet frit is observed to play an important role in determining ... Capillary columns packed electrokinetically with 1.5-μm nonporous octadecylsilica particles are used to achieve rapid ... Advances in Capillary Electrochromatography: Rapid and High-Efficiency Separations of PAHs. *. Rajeev Dadoo. , Richard N. Zare ... inproceedings{Dadoo1998AdvancesIC, title={Advances in Capillary Electrochromatography: Rapid and High-Efficiency Separations of ...
more infohttps://www.semanticscholar.org/paper/Advances-in-Capillary-Electrochromatography-Rapid-Dadoo-Zare/fec40d635d334bfdb0bb5d52f18fe69f6f8ae9e2

New particle-loaded monoliths for chiral capillary electrochromatographic separation - Schmid - 2004 - ELECTROPHORESIS - Wiley...New particle-loaded monoliths for chiral capillary electrochromatographic separation - Schmid - 2004 - ELECTROPHORESIS - Wiley...

Capillary-LC versus capillary electrochromatography, Journal of Biochemical and Biophysical Methods, 2007, 70, 1, 77. CrossRef ... Ivan Mikšík, Pavla Sedláková, Capillary electrochromatography of proteins and peptides, Journal of Separation Science, 2007, 30 ... Dorothee Wistuba, Volker Schurig, Comparison of monolithic approaches for enantioselective capillary electrochromatography ... Monoliths with chiral surface functionalization for enantioselective capillary electrochromatography, Journal of Pharmaceutical ...
more infohttp://onlinelibrary.wiley.com/doi/10.1002/elps.200406038/abstract

eCite - On-Column Ion-Exchange Preconcentration of Inorganic Anions in Open Tubular Capillary Electrochromatography with...eCite - On-Column Ion-Exchange Preconcentration of Inorganic Anions in Open Tubular Capillary Electrochromatography with...

On-Column Ion-Exchange Preconcentration of Inorganic Anions in Open Tubular Capillary Electrochromatography with Elution Using ... On-Column Ion-Exchange Preconcentration of Inorganic Anions in Open Tubular Capillary Electrochromatography with Elution Using ... On-Column Ion-Exchange Preconcentration of Inorganic Anions in Open Tubular Capillary Electrochromatography with Elution Using ... introducing over 40 capillary volumes of sample. A method based on a 10-min injection allowed a 100-fold increase in ...
more infohttp://ecite.utas.edu.au/26114

Capillary Electrophoresis in the Early Twenty-First Century: New Trends and Relevant Applications - Nova Science PublishersCapillary Electrophoresis in the Early Twenty-First Century: New Trends and Relevant Applications - Nova Science Publishers

... capillary electrochromatography, capillary isoelectric focusing, isotachophoresis, non-aqueous capillary electrophoresis, etc ... Chapter 5. New Trends in Capillary Electrochromatography Techniques. (Chiara Fanali and Giovanni DOrazio, Unit of Food Science ... Keywords: Capillary Electrophoresis, Capillary Electrophoresis-Mass Spectrometry, Food Analysis, Environmental Analysis, ... Moreover, the versatility of CE operational modes (capillary zone electrophoresis, micellar electrokinetic capillary ...
more infohttps://novapublishers.com/shop/capillary-electrophoresis-in-the-early-twenty-first-century-new-trends-and-relevant-applications/

中国科学院大连化学物理研究所机构知识库(DICP OpenIR): Fast analysis of capillary electrochromatography with non-porous ODS as中国科学院大连化学物理研究所机构知识库(DICP OpenIR): Fast analysis of capillary electrochromatography with non-porous ODS as

High-speed capillary electrochromatography was developed on both short and long packed columns with 2 mu m non-porous ODS as ... capillary electrochromatography ; fast analysis ; non-porous ODS ; aromatic compounds ; DNPH derived aldehydes and ketones ... Fast analysis of capillary electrochromatography with non-porous ODS as the stationary phase[J]. ANALYTICAL LETTERS,1999,32(13 ... Fast analysis of capillary electrochromatography with non-porous ODS as the stationary phase. ...
more infohttp://cas-ir.dicp.ac.cn/handle/321008/85555

Publication : USDA ARSPublication : USDA ARS

Separations of the vitamin-E active compounds in edible oils were evaluated by capillary electrochromatography. The new high- ... or silica were studied by capillary electrochromatography (CEC)-UV detection. The homologues and isomers of the vitamin E- ... Title: CAPILLARY ELECTROCHROMATOGRAPHIC EVALUATION OF VITAMIN E ACTIVE OIL CONSTITUENTS-TOCOPHEROLS AND TOCOTRIENOLS ...
more infohttps://www.ars.usda.gov/research/publications/publication/?seqNo115=113550

Oxalate induces breast cancer | BMC Cancer | Full TextOxalate induces breast cancer | BMC Cancer | Full Text

We combined Capillary Electrochromatography, histology, and gene expression (qRT-PCR) to analyze patient-matched normal breast ... We combined Capillary Electrochromatography, histology, and gene expression (qRT-PCR) to analyze patient-matched normal breast ... The supernatant fractions were analyzed by Capillary Electrochromatography to establish the total concentration of oxalate ... The supernatant fractions were analyzed by Capillary Electrochromatography to establish the total concentration of Oxalate ...
more infohttps://bmccancer.biomedcentral.com/articles/10.1186/s12885-015-1747-2

Reversed phase continuous full filling CEC-ESI-MS | Lund University Diabetes CentreReversed phase continuous full filling CEC-ESI-MS | Lund University Diabetes Centre

Nanoparticles were used as a pseudostationary phase in capillary electrochromatography (CEC) electrospray ionisation-mass ...
more infohttps://www.ludc.lu.se/peter-spegel-associate-professor/publication/c3f69179-f6a3-4bef-9c15-4de68c9b8c01

May 2016 - Calcium Channel Mutations in Cardiac Arrhythmia SyndromesMay 2016 - Calcium Channel Mutations in Cardiac Arrhythmia Syndromes

... technical advancements of CE capillary gel electrophoresis capillary isoelectric concentrating capillary electrochromatography ...
more infohttp://www.researchdataservice.com/2016/05/

A critical evaluation of the application of capillary electrophoresis to the detection and determination of 1,4-benzodiazepine...A critical evaluation of the application of capillary electrophoresis to the detection and determination of 1,4-benzodiazepine...

It is anticipated that, with the interfacing of CZE: and capillary electrochromatography (CEC) with mass spectrometry (MS) ... Studies of the capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MEKC) behaviour of 1, ... The capillary electrophoresis (CE) techniques, which generally have inferior limits of detection (LOD) to rival techniques such ... Smyth, Franklin and McClean, Stephen (1998) A critical evaluation of the application of capillary electrophoresis to the ...
more infohttp://uir.ulster.ac.uk/1107/

Capillary electrochromatography - WikipediaCapillary electrochromatography - Wikipedia

In capillary electrochromatography capillaries, packed with HPLC stationary phase, are subjected to a high voltage. Separation ... "Capillary electrochromatography". The Analyst. 123 (7): 87-102. doi:10.1039/a801148f. ISSN 0003-2654. Smith, N. "Capillary ... Capillary electrochromatography (CEC) combines the principles used in HPLC and CE. The mobile phase is driven across the ... Capillary electrochromatography (CEC) is a chromatographic technique in which the mobile phase is driven through the ...
more infohttps://en.wikipedia.org/wiki/Capillary_electrochromatography

Preparation of Novel Zwitterionic Monolith for Capillary Electrochromatography and Nano LC-MS Applications | SpringerLinkPreparation of Novel Zwitterionic Monolith for Capillary Electrochromatography and Nano LC-MS Applications | SpringerLink

Hydrophilic interaction chromatography Capillary electrochromatography Neurotransmitters Zwitterionic monolithic column ... Preparation of Novel Zwitterionic Monolith for Capillary Electrochromatography and Nano LC-MS Applications. ... nucleosides and nucleotides for capillary electrochromatography (CEC). Relative standard deviations (RSD%) of the retention ...
more infohttps://link.springer.com/article/10.1007%2Fs10337-019-03823-9

Rapid separation and determination of structurally related anthraquinones in Rhubarb by pressurized capillary...Rapid separation and determination of structurally related anthraquinones in Rhubarb by pressurized capillary...

A pressurized capillary electrochromatography (pCEC) with monolithic column has been developed for the rapid separation and ... separation and determination of structurally related anthraquinones in Rhubarb by pressurized capillary electrochromatography. ...
more infohttps://www.sigmaaldrich.com/catalog/papers/16875795

Continuous full filling capillary electrochromatography: Nanoparticle synthesis and evaluationContinuous full filling capillary electrochromatography: Nanoparticle synthesis and evaluation

... Spégel, Peter LU ; Viberg, ... continuous full filling, electrospray ionisation, orthogonal, capillary electrochromatography, mass spectrometry, ... Reversed phase continuous full filling capillary electrochromatography with electrospray ionisation mass spectrometric ... Reversed phase continuous full filling capillary electrochromatography with electrospray ionisation mass spectrometric ...
more infohttps://lup.lub.lu.se/search/publication/648052

Open-tubular capillary electrochromatography of bovine beta-lactoglobulin variants A and B using an aptamer stationary phase |...Open-tubular capillary electrochromatography of bovine beta-lactoglobulin variants A and B using an aptamer stationary phase |...

Open-tubular capillary electrochromatography of bovine beta-lactoglobulin variants A and B using an aptamer stationary phase. ... Silinski, M., & McGown, L. (2001). Open-tubular capillary electrochromatography of bovine beta-lactoglobulin variants A and B ...
more infohttps://www.rti.org/publication/open-tubular-capillary-electrochromatography-bovine-beta-lactoglobulin-variants-and-b

An Integrated Fritless Colum for On-Chip Capillary Electrochromatography with Conventional Stationary Phases - InfoscienceAn Integrated Fritless Colum for On-Chip Capillary Electrochromatography with Conventional Stationary Phases - Infoscience

... capillary electrochromatography (CEC) has been realized. The structure includes an injector and a tapered column in which the ... and tested using a capillary electrophoretic separation of FITC-labeled amino acids. To perform CEC, the separation channel was ... Home , An Integrated Fritless Colum for On-Chip Capillary Electrochromatography with Conventional Stationary Phases , HTML MARC ... capillary electrochromatography (CEC) has been realized. The structure includes an injector and a tapered column in which the ...
more infohttps://infoscience.epfl.ch/record/137589/export/hm

Open-tubular ion-exchange capillary electrochromatography of inorganic anions  - Open Access RepositoryOpen-tubular ion-exchange capillary electrochromatography of inorganic anions - Open Access Repository

Breadmore, MC, Macka, M, Haddad, PR and Avdalovic, N 2000 , Open-tubular ion-exchange capillary electrochromatography of ... The coated capillary showed reversed electro-osmotic flow (EOF), allowing anions to be separated in the co-EOF mode. The latex ... Open-tubular IE-CEC columns were prepared by coating the inner wall of a fused silica capillary with quaternary ammonium anion- ... The effective ion-exchange capacity of the capillary, and also the resulting anion-exchange interactions with the analytes, ...
more infohttps://eprints.utas.edu.au/6347/

Splitless on-line coupling of capillary high-performance liquid chromatography, capillary electrochromatography and pressurized...Splitless on-line coupling of capillary high-performance liquid chromatography, capillary electrochromatography and pressurized...

... capillary electrochromatography (CEC) and pressurized capillary electrochromatography (pCEC) could be applied. The detection ... capillary electrochromatography and pressurized capillary electrochromatography with nuclear magnetic resonance spectroscopy. ... capillary electrochromatography and pressurized capillary electrochromatography with nuclear magnetic resonance spectroscopy ... With the employed apparatus configuration different capillary separation techniques such as capillary high-performance liquid ...
more infohttp://connection.ebscohost.com/c/articles/15124645/splitless-on-line-coupling-capillary-high-performance-liquid-chromatography-capillary-electrochromatography-pressurized-capillary-electrochromatography-nuclear-magnetic-resonance-spectroscopy

Synthesis and Characterization of Reversed-Phase Continuous Beds for Isocratic and Gradient Capillary Liquid Chromatography and...Synthesis and Characterization of Reversed-Phase Continuous Beds for Isocratic and Gradient Capillary Liquid Chromatography and...

Reversed-Phase Continuous Beds for Isocratic and Gradient Capillary Liquid Chromatography and Capillary Electrochromatography ...
more infohttp://uu.diva-portal.org/smash/record.jsf?pid=diva2:104287

Capillary electrochromatography of inorganic and small organic anions using pseudo and wall-coated ion exchange phases  - Open...Capillary electrochromatography of inorganic and small organic anions using pseudo and wall-coated ion exchange phases - Open...

Capillary electrochromatography of inorganic and small organic anions using pseudo and wall-coated ion exchange phases ... Breadmore, MC 2001 , Capillary electrochromatography of inorganic and small organic anions using pseudo and wall-coated ion ... ion-exchange (IE) phases for the separation of anions by ion-exchange capillary. electrochromatography (IE-CEC).. The viability ... enable the on-capillary preconcentration of inorganic anions via IE interactions. A. new elution method, namely the use of a ...
more infohttps://eprints.utas.edu.au/19124/

Two-Dimensional Electrochromatography/Capillary Electrophoresis on a MicrochipTwo-Dimensional Electrochromatography/Capillary Electrophoresis on a Microchip

Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
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  • Capillary columns packed electrokinetically with 1.5-μm nonporous octadecylsilica particles are used to achieve rapid separations with high efficiencies. (semanticscholar.org)
  • Separations of the vitamin-E active compounds in edible oils were evaluated by capillary electrochromatography. (usda.gov)
  • Separations of lipid antioxidants, tocopherols (T), and tocotrienols (T3) on octylsilica (OS), octadecylsilica (ODS), phenylsilica, or silica were studied by capillary electrochromatography (CEC)-UV detection. (usda.gov)
  • A polymer-based neutral monolithic capillary column was prepared by radical polymerization of glycidyl methacrylate and ethylene dimethacrylate in a 100 lam id fused-silica capillary, and the prepared monolithic column was subsequently modified based on a ring opening reaction of epoxide groups with 1 M lysine in solution (pH 8.0) at 75 degrees C for 10 h to produce a lysine chemically bonded stationary phases in capillary column. (dicp.ac.cn)
  • In the early development of capillary UHPLC, home-built instruments were constructed that eliminated nearly all instrumental effects and gave a direct measure of column efficiency while also allowing for backpressures in excess of 50,000 psi [ 22 , 23 , 24 , 25 , 26 , 27 ]. (mdpi.com)
  • article{7f6274d2-59e3-4705-a96e-f4bb0009f833, abstract = {Reversed phase continuous full filling capillary electrochromatography with electrospray ionisation mass spectrometric detection was performed with highly sulphated poly [styrene-co-(lauryl methacrylate)-co-(divinylbenzene)] nanoparticles. (lu.se)
  • Popular Abstract in Swedish Vad arbetar en analytisk kemist med? (dissertations.se)
  • Open-tubular IE-CEC columns were prepared by coating the inner wall of a fused silica capillary with quaternary ammonium anion-exchange latex particles (approximately 75 nm in diameter) bound electrostatically to the wall. (edu.au)
  • The latex particles on the capillary wall introduced anion-exchange interactions with the analytes, the strength of which could be moderated by the type and concentration of the competing ion used in the background electrolyte (BGE). (edu.au)
  • The effective ion-exchange capacity of the capillary, and also the resulting anion-exchange interactions with the analytes, could be increased by decreasing the capillary diameter. (edu.au)
  • In this study, the separation of acidic compounds by strong anion-exchange capillary electrochromatography (SAX-eEC) was carried cut. (dict.cn)
  • The advantage of having a heterogeneous phase in an OT column was exploited to enable the on-capillary preconcentration of inorganic anions via IE interactions. (edu.au)
  • A solid-phase extraction method based on an ionexchange retention mechanism has been used for in-line preconcentration of inorganic anions prior to their separation by capillary electrophoresis (CE). (edu.au)
  • A single capillary containing a preconcentration and a separation zone has been used in a commercial CE instrument without instrumental modification. (edu.au)
  • For the first time, a multi-lumen capillary (MLC) (126 parallel channels of 4.2 µm i.d) has been modified to produce a C18-functionalised silica porous layer open tubular (PLOT) capillary column for both on-capillary preconcentration and separation. (springer.com)
  • Capillary electrochromatography (CEC) is a chromatographic technique in which the mobile phase is driven through the chromatographic bed by electroosmosis. (wikipedia.org)
  • Very recently, we developed a multidimensional platform composed of capillary isoelectric focusing and CEC for human serum proteomics profiling. (okstate.edu)
  • The coated capillary showed reversed electro-osmotic flow (EOF), allowing anions to be separated in the co-EOF mode. (edu.au)
  • Due to the presence of a stationary phase (SP) inside a capillary and the use of an electro-osmotic ow (EOF) as a driving force for the mobile phase, the user can bene t from properties, such as a high sample loading capacity, high ef ciencies, high peak capacities, and low sample and solvent consumptions . (taylorfrancis.com)
  • Capillary electrophoresis (CE) is a powerful analytical technique that is widely used in research and development and in quality control of pharmaceuticals. (elsevier.com)
  • Fast analysis of capillary electrochromatography with non-porous ODS as the stationary phase[J]. ANALYTICAL LETTERS,1999,32(13):2679-2690. (dicp.ac.cn)
  • Meantime, the DMMPPS-based monolith exhibited good mechanical stability and excellent separation performance for nucleic acid bases, nucleosides and nucleotides for capillary electrochromatography (CEC). (springer.com)
  • Xie, Z. Preparation and evaluation of o-phenanthroline immobilized on a hybrid silica monolith modified with ionic liquids for reversed-phase pressurized capillary electrochromatography. (alfa.com)
  • Finally, direct coupling of the PLOT-MLC with the QTOF via a capillary electrosprayer facilitated the simultaneous use of the modified capillary as a solid-phase concentrator, separator column (carrying out concentration-focusing-separation on the PLOT-MLC) and electrospray emitter. (springer.com)
  • Theory of column optimization with limited pressure and analysis time, and fabrication of chemically bonded reversed-phase columns on etched borosilicate glass capillaries. (springer.com)
  • Ultra-Fast Analysis of Allergens using Capillary Elect. (selectscience.net)
  • Increasing the concentration of the competing ion resulted in a decrease in the ion-exchange interactions and a change in the migration order from that obtained by capillary electrophoresis using a high ionic BGE. (edu.au)