A species of bacteria that resemble small tightly coiled spirals. Its organisms are known to cause abortion in sheep and fever and enteritis in man and may be associated with enteric diseases of calves, lambs, and other animals.
Infections with bacteria of the genus CAMPYLOBACTER.
A genus of bacteria found in the reproductive organs, intestinal tract, and oral cavity of animals and man. Some species are pathogenic.
A species of bacteria present in man and many kinds of animals and birds, often causing infertility and/or abortion.
A species of gram-negative, rod-shaped bacteria isolated from the intestinal tract of swine, poultry, and man. It may be pathogenic.
Inflammation of any segment of the SMALL INTESTINE.
An acute inflammatory autoimmune neuritis caused by T cell- mediated cellular immune response directed towards peripheral myelin. Demyelination occurs in peripheral nerves and nerve roots. The process is often preceded by a viral or bacterial infection, surgery, immunization, lymphoma, or exposure to toxins. Common clinical manifestations include progressive weakness, loss of sensation, and loss of deep tendon reflexes. Weakness of respiratory muscles and autonomic dysfunction may occur. (From Adams et al., Principles of Neurology, 6th ed, pp1312-1314)
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild.
A species of thermophilic CAMPYLOBACTER found in healthy seagulls and causing ENTERITIS in humans.
A protein with a molecular weight of 40,000 isolated from bacterial flagella. At appropriate pH and salt concentration, three flagellin monomers can spontaneously reaggregate to form structures which appear identical to intact flagella.
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.
Diseases characterized by injury or dysfunction involving multiple peripheral nerves and nerve roots. The process may primarily affect myelin or nerve axons. Two of the more common demyelinating forms are acute inflammatory polyradiculopathy (GUILLAIN-BARRE SYNDROME) and POLYRADICULONEUROPATHY, CHRONIC INFLAMMATORY DEMYELINATING. Polyradiculoneuritis refers to inflammation of multiple peripheral nerves and spinal nerve roots.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Domesticated birds raised for food. It typically includes CHICKENS; TURKEYS, DUCKS; GEESE; and others.
Proteins found in any species of bacterium.
Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.
An increased liquidity or decreased consistency of FECES, such as running stool. Fecal consistency is related to the ratio of water-holding capacity of insoluble solids to total water, rather than the amount of water present. Diarrhea is not hyperdefecation or increased fecal weight.
Food products manufactured from poultry.
A variant of the GUILLAIN-BARRE SYNDROME characterized by the acute onset of oculomotor dysfunction, ataxia, and loss of deep tendon reflexes with relative sparing of strength in the extremities and trunk. The ataxia is produced by peripheral sensory nerve dysfunction and not by cerebellar injury. Facial weakness and sensory loss may also occur. The process is mediated by autoantibodies directed against a component of myelin found in peripheral nerves. (Adams et al., Principles of Neurology, 6th ed, p1313; Neurology 1987 Sep;37(9):1493-8)
The blind sac or outpouching area of the LARGE INTESTINE that is below the entrance of the SMALL INTESTINE. It has a worm-like extension, the vermiform APPENDIX.
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
Places where animals are slaughtered and dressed for market.
INFLAMMATION of any segment of the GASTROINTESTINAL TRACT from ESOPHAGUS to RECTUM. Causes of gastroenteritis are many including genetic, infection, HYPERSENSITIVITY, drug effects, and CANCER.
A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
A dilated cavity extended caudally from the hindgut. In adult birds, reptiles, amphibians, and many fishes but few mammals, cloaca is a common chamber into which the digestive, urinary and reproductive tracts discharge their contents. In most mammals, cloaca gives rise to LARGE INTESTINE; URINARY BLADDER; and GENITALIA.
Salts and esters of hippuric acid.
A genus of gram-negative, aerotolerant, spiral-shaped bacteria isolated from water and associated with diarrhea in humans and animals.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
The functional hereditary units of BACTERIA.
Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.
The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
The edible portions of any animal used for food including domestic mammals (the major ones being cattle, swine, and sheep) along with poultry, fish, shellfish, and game.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Substances that reduce the growth or reproduction of BACTERIA.
Animals which have become adapted through breeding in captivity to a life intimately associated with humans. They include animals domesticated by humans to live and breed in a tame condition on farms or ranches for economic reasons, including LIVESTOCK (specifically CATTLE; SHEEP; HORSES; etc.), POULTRY; and those raised or kept for pleasure and companionship, e.g., PETS; or specifically DOGS; CATS; etc.
Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.
A bacteriostatic antibiotic macrolide produced by Streptomyces erythreus. Erythromycin A is considered its major active component. In sensitive organisms, it inhibits protein synthesis by binding to 50S ribosomal subunits. This binding process inhibits peptidyl transferase activity and interferes with translocation of amino acids during translation and assembly of proteins.
Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).
Acute illnesses, usually affecting the GASTROINTESTINAL TRACT, brought on by consuming contaminated food or beverages. Most of these diseases are infectious, caused by a variety of bacteria, viruses, or parasites that can be foodborne. Sometimes the diseases are caused by harmful toxins from the microbes or other chemicals present in the food. Especially in the latter case, the condition is often called food poisoning.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Direct nucleotide sequencing of gene fragments from multiple housekeeping genes for the purpose of phylogenetic analysis, organism identification, and typing of species, strain, serovar, or other distinguishable phylogenetic level.
A broad-spectrum antimicrobial carboxyfluoroquinoline.
The structure of one molecule that imitates or simulates the structure of a different molecule.
The presence in food of harmful, unpalatable, or otherwise objectionable foreign substances, e.g. chemicals, microorganisms or diluents, before, during, or after processing or storage.
A species of CAMPYLOBACTER isolated from the INTESTINES of PIGS with proliferative ENTERITIS. It is also found in CATTLE and in CRICETINAE and can cause enteritis in humans.
The expelling of bacteria from the body. Important routes include the respiratory tract, genital tract, and intestinal tract.
Techniques used in studying bacteria.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
A specific monosialoganglioside that accumulates abnormally within the nervous system due to a deficiency of GM1-b-galactosidase, resulting in GM1 gangliosidosis.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A subclass of ACIDIC GLYCOSPHINGOLIPIDS. They contain one or more sialic acid (N-ACETYLNEURAMINIC ACID) residues. Using the Svennerholm system of abbrevations, gangliosides are designated G for ganglioside, plus subscript M, D, or T for mono-, di-, or trisialo, respectively, the subscript letter being followed by a subscript arabic numeral to indicated sequence of migration in thin-layer chromatograms. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1997)
Any type of abortion, induced or spontaneous, that is associated with infection of the UTERUS and its appendages. It is characterized by FEVER, uterine tenderness, and foul discharge.
A species of CAMPYLOBACTER isolated from cases of human PERIODONTITIS. It is a microaerophile, capable of respiring with OXYGEN.
A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.
Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The genetic complement of a BACTERIA as represented in its DNA.
Any aspect of the operations in the preparation, processing, transport, storage, packaging, wrapping, exposure for sale, service, or delivery of food.
The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.
A family of hemoglobin-like proteins found in BACTERIA; PLANTS; and unicellular eukaryotes. Truncated hemoglobins are distantly related to vertebrate hemoglobins and are typically shorter than vertebrate hemoglobins by 20-40 residues.
The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.
The white liquid secreted by the mammary glands. It contains proteins, sugar, lipids, vitamins, and minerals.
Substances elaborated by bacteria that have antigenic activity.
An independent state, an archipelago in the western Persian Gulf, northwest of Qatar. It comprises low-lying islands of Bahrain (the largest), Muharraq, Sitra, and several islets. It has extensive oil fields. The name comes from the Arabic al-bahrayn, "the two seas", with reference to its lying in the middle of a bay with its "two seas" east and west of it. (From Webster's New Geographical Dictionary, 1988, p107 & Room, Brewer's Dictionary of Names, 1992, p45)
Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
Substances that prevent infectious agents or organisms from spreading or kill infectious agents in order to prevent the spread of infection.
Animate or inanimate sources which normally harbor disease-causing organisms and thus serve as potential sources of disease outbreaks. Reservoirs are distinguished from vectors (DISEASE VECTORS) and carriers, which are agents of disease transmission rather than continuing sources of potential disease outbreaks.
Large woodland game BIRDS in the subfamily Meleagridinae, family Phasianidae, order GALLIFORMES. Formerly they were considered a distinct family, Melegrididae.
A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.
Sudden increase in the incidence of a disease. The concept includes EPIDEMICS and PANDEMICS.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Substances that are toxic to cells; they may be involved in immunity or may be contained in venoms. These are distinguished from CYTOSTATIC AGENTS in degree of effect. Some of them are used as CYTOTOXIC ANTIBIOTICS. The mechanism of action of many of these are as ALKYLATING AGENTS or MITOSIS MODULATORS.
A synthetic 1,8-naphthyridine antimicrobial agent with a limited bacteriocidal spectrum. It is an inhibitor of the A subunit of bacterial DNA GYRASE.
The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
The science of breeding, feeding and care of domestic animals; includes housing and nutrition.
The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.
Constituent of 50S subunit of prokaryotic ribosomes containing about 3200 nucleotides. 23S rRNA is involved in the initiation of polypeptide synthesis.

Cloning and expression of the dnaK gene of Campylobacter jejuni and antigenicity of heat shock protein 70. (1/1435)

Campylobacter jejuni is a leading cause of infectious diarrhea throughout the world. In addition, there is growing evidence that Guillain-Barre syndrome, an inflammatory demyelinating disease of the peripheral nervous system, is frequently preceded by C. jejuni infection. In the present study, the hrcA-grpE-dnaK gene cluster of C. jejuni was cloned and sequenced. The dnaK gene consists of an open reading frame of 1,869 bp and encodes a protein with a high degree of homology to other bacterial 70-kDa heat shock proteins (HSPs). The overall percentages of identity to the HSP70 proteins of Helicobacter pylori, Borrelia burgdorferi, Chlamydia trachomatis, and Bacillus subtilis were calculated to be 78.1, 60.5, 57.2, and 53. 8%, respectively. Regions similar to the Escherichia coli sigma70 promoter consensus sequence and to a cis-acting regulatory element (CIRCE) are located upstream of the hrcA gene. Following heat shock, a rapid increase of dnaK mRNA was detectable, which reached its maximum after 20 to 30 min. A 6-His-tagged recombinant DnaK protein (rCjDnaK-His) was generated in E. coli, after cloning of the dnaK coding region into pET-22b(+), and purified by affinity and gel filtration chromatography. Antibody responses to rCjDnaK-His were significantly elevated, compared to those of healthy individuals, in about one-third of the serum specimens obtained from C. jejuni enteritis patients.  (+info)

Physiological characterization of viable-but-nonculturable Campylobacter jejuni cells. (2/1435)

Campylobacter jejuni is a pathogenic, microaerophilic, gram-negative, mesophilic bacterium. Three strains isolated from humans with enteric campylobacteriosis were able to survive at high population levels (10(7) cells ml-1) as viable-but-nonculturable (VBNC) forms in microcosm water. The VBNC forms of the three C. jejuni strains were enumerated and characterized by using 5-cyano-2,3-ditolyl tetrazolium chloride-4',6-diamino-2-phenylindole staining. Cellular volume, adenylate energy charge, internal pH, intracellular potassium concentration, and membrane potential values were determined in stationary-phase cell suspensions after 48 h of culture on Columbia agar and after 1 to 30 days of incubation in microcosm water and compared. A notable increase in cell volume was observed with the VBNC state; the average cell volumes were 1.73 microliter mg of protein-1 for the culturable form and 10.96 microliter mg of protein-1 after 30 days of incubation in microcosm water. Both the internal potassium content and the membrane potential were significantly lower in the VBNC state than in the culturable state. Culturable cells were able to maintain a difference of 0.6 to 0.9 pH unit between the internal and external pH values; with VBNC cells this difference decreased progressively with time of incubation in microcosm water. Measurements of the cellular adenylate nucleotide concentrations revealed that the cells had a low adenylate energy charge (0.66 to 0.26) after 1 day of incubation in microcosm water, and AMP was the only nucleotide detected in the three strains after 30 days of incubation in microcosm water.  (+info)

Evaluation of accuracy and repeatability of identification of food-borne pathogens by automated bacterial identification systems. (3/1435)

The performances of five automated microbial identification systems, relative to that of a reference identification system, for their ability to accurately and repeatedly identify six common food-borne pathogens were assessed. The systems assessed were the MicroLog system (Biolog Inc., Hayward, Calif.), the Microbial Identification System (MIS; MIDI Inc., Newark, Del.), the VITEK system (bioMerieux Vitek, Hazelwood, Mo.), the MicroScan WalkAway 40 system (Dade-MicroScan International, West Sacramento, Calif.), and the Replianalyzer system (Oxoid Inc., Nepean, Ontario, Canada). The sensitivities and specificities of these systems for the identification of food-borne isolates of Bacillus cereus, Campylobacter jejuni, Listeria monocytogenes, Staphylococcus aureus, Salmonella spp., and verotoxigenic Escherichia coli were determined with 40 reference positive isolates and 40 reference negative isolates for each pathogen. The sensitivities of these systems for the identification of these pathogens ranged from 42.5 to 100%, and the specificities of these systems for the identification of these pathogens ranged from 32.5 to 100%. Some of the systems had difficulty correctly identifying the reference isolates when the results were compared to those from the reference identification tests. The sensitivity of MIS for the identification of S. aureus, B. cereus, E. coli, and C. jejuni, for example, ranged from 47.5 to 72. 5%. The sensitivity of the Microlog system for the identification of E. coli was 72.5%, and the sensitivity of the VITEK system for the identification of B. cereus was 42.5%. The specificities of four of the five systems for the identification of all of the species tested with the available databases were greater than or equal to 97.5%; the exception was MIS for the identification of C. jejuni, which displayed a specificity of 32.5% when it was tested with reference negative isolates including Campylobacter coli and other Campylobacter species. All systems had >80% sensitivities for the identification of Salmonella species and Listeria species at the genus level. The repeatability of these systems for the identification of test isolates ranged from 30 to 100%. Not all systems included all six pathogens in their databases; thus, some species could not be tested with all systems. The choice of automated microbial identification system for the identification of a food-borne pathogen would depend on the availability of identification libraries within the systems and the performance of the systems for the identification of the pathogen.  (+info)

Cloning, mutation and distribution of a putative lipopolysaccharide biosynthesis locus in Campylobacter jejuni. (4/1435)

A region encoding ORFs with homology to known lipopolysaccharide (LPS) biosynthesis genes was isolated from two strains of Campylobacter jejuni. One of the strains produces LPS, but the second strain is reported to produce only lipooligosaccharide (LOS) and therefore lacks the O-chain. The two strains shared six predicted ORFs, but an additional ORF, orfE, of unknown function was identified in the LOS-producing strain. Mutation of the shared wbeE (rfbE) homologue (orfF) or deletion of five of the seven genes reduced core reactivity with specific antiserum without affecting O-chain production. Mutation of either the capD homologue (orfG) or the unique orfE had no detectable effect on LOS or LPS production. The presence or absence of orfE in 36 isolates of C. jejuni did not correlate with LOS/LPS phenotype or serotype. However, after insertion of orfE into a LPS-producing orfE-negative strain the O-chain ladder was no longer detectable on Western blots. We were not able to disrupt the wbaP (rfbP) homologue (orfC) in C jejuni.  (+info)

Campylobacter jejuni--an emerging foodborne pathogen. (5/1435)

Campylobacter jejuni is the most commonly reported bacterial cause of foodborne infection in the United States. Adding to the human and economic costs are chronic sequelae associated with C. jejuni infection--Guillian-Barre syndrome and reactive arthritis. In addition, an increasing proportion of human infections caused by C. jejuni are resistant to antimicrobial therapy. Mishandling of raw poultry and consumption of undercooked poultry are the major risk factors for human campylobacteriosis. Efforts to prevent human illness are needed throughout each link in the food chain.  (+info)

The risk of Guillain-Barre syndrome following infection with Campylobacter jejuni. (6/1435)

To estimate the incidence of Guillain-Barre syndrome (GBS) following Campylobacter jejuni infection (CI) we studied three populations where outbreaks of CI had occurred involving an estimated 8000 cases. No case of GBS was detected in the 6 months following the outbreaks in the local populations. The point estimate for the risk of GBS following CI estimated in this study was 0 in 8000 (95% confidence interval 0-3).  (+info)

Detection of cytolethal distending toxin activity and cdt genes in Campylobacter spp. isolated from chicken carcasses. (7/1435)

This study was designed to determine whether isolates from chicken carcasses, the primary source of Campylobacter jejuni and Campylobacter coli in human infections, commonly carry the cdt genes and also whether active cytolethal distending toxin (CDT) is produced by these isolates. Campylobacter spp. were isolated from all 91 fresh chicken carcasses purchased from local supermarkets. Campylobacter spp. were identified on the basis of both biochemical and PCR tests. Of the 105 isolates, 70 (67%) were identified as C. jejuni, and 35 (33%) were identified as C. coli. PCR tests amplified portions of the cdt genes from all 105 isolates. Restriction analysis of PCR products indicated that there appeared to be species-specific differences between the C. jejuni and C. coli cdt genes, but that the restriction patterns of the cdt genes within strains of the same species were almost invariant. Quantitation of active CDT levels produced by the isolates indicated that all C. jejuni strains except four (94%) had mean CDT titers greater than 100. Only one C. jejuni strain appeared to produce no active CDT. C. coli isolates produced little or no toxin. These results confirm the high rate of Campylobacter sp. contamination of fresh chicken carcasses and indicate that cdt genes may be universally present in C. jejuni and C. coli isolates from chicken carcasses.  (+info)

Detection of small numbers of Campylobacter jejuni and Campylobacter coli cells in environmental water, sewage, and food samples by a seminested PCR assay. (8/1435)

A rapid and sensitive assay was developed for detection of small numbers of Campylobacter jejuni and Campylobacter coli cells in environmental water, sewage, and food samples. Water and sewage samples were filtered, and the filters were enriched overnight in a nonselective medium. The enrichment cultures were prepared for PCR by a rapid and simple procedure consisting of centrifugation, proteinase K treatment, and boiling. A seminested PCR based on specific amplification of the intergenic sequence between the two Campylobacter flagellin genes, flaA and flaB, was performed, and the PCR products were visualized by agarose gel electrophoresis. The assay allowed us to detect 3 to 15 CFU of C. jejuni per 100 ml in water samples containing a background flora consisting of up to 8, 700 heterotrophic organisms per ml and 10,000 CFU of coliform bacteria per 100 ml. Dilution of the enriched cultures 1:10 with sterile broth prior to the PCR was sometimes necessary to obtain positive results. The assay was also conducted with food samples analyzed with or without overnight enrichment. As few as +info)

Dive into the research topics of Adaptation of Campylobacter jejuni NCTC11168 to high-level colonization of the avian gastrointestinal tract. Together they form a unique fingerprint. ...
Campylobacter jejuni subsp. jejuni ATCC ® 43432D-5™ Designation: Genomic DNA from Campylobacter jejuni subsp. jejuni Strain MK7 TypeStrain=False Application:
Campylobacter jejuni subsp. jejuni ATCC ® 43446D-5™ Designation: Genomic DNA from Campylobacter jejuni subsp. jejuni Strain MK104 TypeStrain=False Application:
An example of the difficulty and complexity associated with pseudogene designation is observed when viewing the CDSs Cj0522, Cj0523 and Cj0524 within C. jejuni NCTC11168. These three CDSs are represented as one whole CDS on a single frame within C. jejuni RM1221 (Cje0628). The three CDSs are large enough to be represented as individual CDSs and in C. jejuni NCTC11168 have been represented on more than one frame. The question can be asked as to whether these CDSs (which are intact in C. jejuni RM1221), represent a pseudogene in C. jejuni NCTC11168. Given the fact that in C. jejuni RM1221 these three CDSs do actually code for a product (Na/Pi-cotransporter, putative), it is more likely that they represent a pseudogene in C. jejuni NCTC11168. In this re-annotation, our intention was to carry out a full mark up of existing pseudogenes, however, the potential for a pseudogene has been noted.. The frequency and importance of pseudogene formation in microorganisms has attained added significance in ...
A study by researchers from the University of Arizona Mel and Enid Zuckerman College of Public Health and the Department of Soil, Water and Environmental Science found the use of disinfectant wipes after the preparation of raw chicken meat reduces the risk of Campylobacter jejuni infections. The study was published in the Journal of Applied Microbiology.. The researchers conducted a quantitative microbial risk assessment and forecasted the exposure to Campylobacter jejuni contaminated surfaces during preparation of chicken fillets and how using a disinfectant-wipe intervention to clean a contaminated work area decreases the risk of infection following the preparation of raw chicken fillet in a domestic kitchen.. Using a Monte Carlo simulation of the risk of transferring Camp. jejuni strain A3249, from various surfaces to hands and subsequently transferring it to the mouth was forecasted. The use of a disinfectant-wipe intervention to disinfect contaminated surface area was also assessed. Several ...
Campylobacter jejuni infection causes cramping, diarrhea, abdominal pain and fever within 2 to 5 days after a person has been exposed to the organism. Campylobacter jejuni is one of the most common bacterial causes of diarrhea. Most cases of Campylobacter jejuni come from handling or ingesting raw or undercooked poultry meat. Although poultry and other birds are not affected by the bacterium, other animals can be. Therefore, it is possible for a person to acquire the infection from contact with infected stool of an ill cat or dog. This is what campylobacter organisms look like through a microscope. (Image courtesy of the Centers for Disease Control and Prevention ...
TY - JOUR. T1 - Tracing isolates from domestic human Campylobacter jejuni infections to chicken slaughter batches and swimming water using whole-genome multilocus sequence typing. AU - Kovanen, Sara. AU - Kivisto, Rauni. AU - Llarena, Ann-Katrin. AU - Zhang, Ji. AU - Karkkainen, Ulla-Maija. AU - Tuuminen, Tamara. AU - Uksila, Jaakko. AU - Hakkinen, Marjaana. AU - Rossi, Mirko. AU - Hanninen, Marja-Liisa. PY - 2016/6/2. Y1 - 2016/6/2. KW - Domestically acquired campylobacteriosis. KW - Summer peak. KW - Chicken. KW - Surface water. KW - Source. KW - SEASONAL PEAK. KW - FINLAND. KW - DISEASE. KW - GENOTYPES. KW - POULTRY. KW - HOST. KW - MLST. KW - 1183 Plant biology, microbiology, virology. KW - 3142 Public health care science, environmental and occupational health. U2 - 10.1016/j.ijfoodmicro.2016.03.009. DO - 10.1016/j.ijfoodmicro.2016.03.009. M3 - Article. VL - 226. SP - 53. EP - 60. JO - International Journal of Food Microbiology. JF - International Journal of Food Microbiology. SN - ...
TY - JOUR. T1 - Population structure and attribution of human clinical Campylobacter jejuni isolates from central Europe to livestock and environmental sources. AU - Kovac, J.. AU - Stessl, B.. AU - Čadež, N.. AU - Gruntar, I.. AU - Cimerman, M.. AU - Stingl, K.. AU - Lušicky, M.. AU - Ocepek, M.. AU - Wagner, M.. AU - Smole Možina, S.. PY - 2018/2/1. Y1 - 2018/2/1. N2 - Campylobacter jejuni is among the most prevalent causes of human bacterial gastroenteritis worldwide. Domesticated animals and, especially, chicken meat are considered to be the main sources of infections. However, the contribution of surface waters and wildlife in C. jejuni transmission to humans is not well understood. We have evaluated the source attribution potential of a six-gene multiplex PCR (mPCR) method coupled with STRUCTURE analysis on a set of 410 C. jejuni strains isolated from environment, livestock, food and humans in central Europe. Multiplex PCR fingerprints were analysed using Subclade prediction algorithm ...
TY - JOUR. T1 - Importance of Campylobacter jejuni FliS and FliW in flagella biogenesis and flagellin secretion. AU - Radomska, Katarzyna A.. AU - Wösten, Marc M.S.M.. AU - Ordoñez, Soledad R.. AU - Wagenaar, Jaap A.. AU - van Putten, Jos P.M.. PY - 2017. Y1 - 2017. N2 - Flagella-driven motility enables bacteria to reach their favorable niche within the host. The human foodborne pathogen Campylobacter jejuni produces two heavily glycosylated structural flagellins (FlaA and FlaB) that form the flagellar filament. It also encodes the non-structural FlaC flagellin which is secreted through the flagellum and has been implicated in host cell invasion. The mechanisms that regulate C. jejuni flagellin biogenesis and guide the proteins to the export apparatus are different from those in most other enteropathogens and are not fully understood. This work demonstrates the importance of the putative flagellar protein FliS in C. jejuni flagella assembly. A constructed fliS knockout strain was non-motile, ...
Campylobacter jejuni is a Gram negative bacteria and is commonly found in the feces of animals and in the gastrointestinal tract of many birds. It can be transmitted to humans as a result of consumption of contaminated drinking water or food or unpasteurized milk. Another common cause of transmission is incorrectly prepared or inadequately cooked poultry and meat products. Campylobacter jejuni is one of the leading causes of gastroenteritis and food poisoning in humans. It causes an infection called Campylobacteriosis. It is basically a food borne illness.. On consumption of contaminated food or drink, you will suffer from enteritis as the bacterium chiefly affects the gut and causes injury in the gut. It invades the epithelial cells of the jejunum, ileum and the colon. You may complain of severe abdominal pain, diarrhoea with or without blood in stools and fever. The symptoms of Campylobacter jejuni infection will last for 24 hours to about a week, in some cases the infection may last for more ...
Campylobacter species are one of the leading causes of foodborne disease in the United States. Campylobacter jejuni and Campylobacter coli are the two main species of concern to human health and cause approximately 95% of human infections. Molecular typing methods, such as pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) are often used to source track foodborne bacterial pathogens. The aim of the present study was to compare PFGE and MLST in typing strains of C. jejuni and C. coli that were isolated from different Oklahoma retail meat sources. A total of 47 Campylobacter isolates (28 C. jejuni and 19 C. coli) isolated from various retail meat samples (beef, beef livers, pork, chicken, turkey, chicken livers, and chicken gizzards) were subjected to pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). PFGE was able to group the 47 Campylobacter isolates into two major clusters (one for C. jejuni and one for C. coli) but failed to differentiate the
Many bacterial pathogens display glycosylated surface structures that contribute to virulence, and targeting these structures is a viable strategy for pathogen control. The foodborne pathogen Campylobacter jejuni expresses a vast diversity of flagellar glycans, and flagellar glycosylation is essential for its virulence. Little is known about why C. jejuni encodes such a diverse set of flagellar glycans, but it has been hypothesized that evolutionary pressure from bacteriophages (phages) may have contributed to this diversity. However, interactions between Campylobacter phages and host flagellar glycans have not been characterized in detail. Previously, we observed that Gp047 (now renamed FlaGrab), a conserved Campylobacter phage protein, binds to C. jejuni flagella displaying the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid, and that this binding partially inhibits cell growth. However, the mechanism of this growth inhibition, as well as how C. jejuni might resist this activity, are not
View more ,Abstract: Campylobacter species are the most common cause of foodborne disease in Australia and many countries throughout the World. Although campylobacteriosis is usually self-limiting, severe cases and those in the young, elderly and immunocompromised require antibiotic therapy. Antibiotic resistant Campylobacter isolates however may prolong illness and increase the risk of invasive disease. Antibiotic resistance in Campylobacter is thought to have arisen through the selective pressure of exposure to antimicrobial agents in veterinary medicine or animal husbandry, leading to the acquisition and dissemination of antibiotic resistance determinants, and genetic elements that harbour such genes, amongst isolates. Little was known about tetracycline and trimethoprim resistance in Australian campylobacters, including the presence of resistance genes and associated genetic elements. Aims of this study were therefore to identify in Australian Campylobacter jejuni and Campylobacter coli ...
The binding of Campylobacter jejuni to fibronectin (Fn), a component of the extracellular matrix, is mediated by a 37 kDa outer-membrane protein termed CadF for Campylobacter adhesion to fibronectin. The specificity of C. jejuni binding to Fn, via CadF, was demonstrated using antibodies reactive against Fn and CadF. More specifically, the anti-CadF antibody reduced the binding of two C. jejuni clinical isolates to immobilized Fn by greater than 50 %. Furthermore, a C. jejuni wild-type isolate, in contrast to the isogenic CadF mutant, was found to compete with another C. jejuni wild-type isolate for host cell receptors. Given the relationship between the pericellular Fn matrix and the cytoskeleton, the involvement of host cell cytoskeletal components in C. jejuni internalization was also examined. Cytochalasin D and mycalolide B microfilament depolymerizing agents resulted in a significant reduction in C. jejuni invasion. Studies targeting paxillin, a focal adhesion signalling molecule, identified an
Reducing Campylobacter spp. carriage in poultry is challenging, but essential to control this major cause of human bacterial gastroenteritis worldwide. Although much is known about the mechanisms and route of Campylobacter spp. colonization in poultry the literature is scarce on antibiotic-free solutions to combat Campylobacter spp. colonization in poultry. In vitro and in vivo studies were conducted to investigate the role of TYPLEX® Chelate (ferric tyrosine), a novel feed additive, in inhibiting Campylobacter jejuni (C. jejuni) biofilm formation and reducing C. jejuni and Escherichia coli (E. coli) colonization in broiler chickens at market age. In an in vitro study, the inhibitory effect on C. jejuni biofilm formation using a plastic bead assay was investigated. The results demonstrated that TYPLEX® Chelate significantly reduces biofilm formation. In an in vivo study, 800 broilers (one-day old) were randomly allocated to 4 dietary treatments in a randomised block design, each having 10 ...
The failure to reduce the Campylobacter contamination of intensively reared poultry may be partially due to Campylobacter resisting disinfection in water after their internalization by waterborne protozoa. Campylobacter jejuni and a variety of waterborne protozoa, including ciliates, flagellates, and alveolates, were detected in the drinking water of intensively reared poultry by a combination of culture and molecular techniques. An in vitro assay showed that C. jejuni remained viable when internalized by Tetrahymena pyriformis and Acanthamoeba castellanii for significantly longer (up to 36 h) than when they were in purely a planktonic state. The internalized Campylobacter were also significantly more resistant to disinfection than planktonic organisms. Collectively, our results strongly suggest that protozoa in broiler drinking water systems can delay the decline of Campylobacter viability and increase Campylobacter disinfection resistance, thus increasing the potential of Campylobacter to ...
The genetic diversity of Campylobacter jejuni isolates from farm animals and their environment was investigated by multilocus sequence typing (MLST). A total of 30 genotypes, defined by allelic profiles (assigned to sequence types [STs]), were found in 112 C. jejuni isolates originating in poultry, cattle, sheep, starlings, and slurry. All but two of these genotypes belonged to one of nine C. jejuni clonal complexes previously identified in isolates from human disease and retail food samples and one clonal complex previously associated with an environmental source. There was some evidence for the association of certain clonal complexes with particular farm animals: isolates belonging to the ST-45 complex predominated among poultry isolates but were absent among sheep isolates, while isolates belonging to the ST-61 and ST-42 complexes were predominant among sheep isolates but were absent from the poultry isolates. In contrast, ST-21 complex isolates were distributed among the different isolation sources.
The bipolar flagella of the foodborne bacterial pathogen Campylobacter jejuni confer motility, which is essential for virulence. The flagella of C. jejuni are post-translationally modified, but how this process is controlled is not well understood. In this work, we have identified a novel PAS-domain containing regulatory system, which modulates flagella-flagella interactions in C. jejuni. Inactivation of the cj1387c gene, encoding a YheO-like PAS6 domain linked to a helix-turn-helix domain, resulted in the generation of a tightly associated
TY - JOUR. T1 - Phenotypic and genetic analyses of campylobacter jejuni lior serotype 76 isolated from chicken meat and clinical specimens. AU - Matsumoto, Masakado. AU - Hiramatsu, Reiji. AU - Yamada, Kazuhiro. AU - Suzuki, Masahiro. AU - Miwa, Yoshio. AU - Yabutani, Mitsutaka. AU - Nagai, Yuhki. AU - Tsuchiya, Michiyo. AU - Noda, Makiko. AU - Nagata, Akihiro. AU - Kawakami, Keiko. AU - Shima, Tomoko. AU - Tatsumi, Norio. AU - Minagawa, Hiroko. PY - 2013. Y1 - 2013. N2 - The aim of this study was to examine the link between Campylobacter jejuni isolates obtained from chicken meat (n = 7) and gastroenteritis patients (n = 744). In total, 751 isolates were subjected to Lior serotyping. All the isolates from chicken meats were serotyped as Lior serotype 76 (LIO76). Among 23 of the identified LIO76 strains, 13 strains (6 from chicken meat and 7 from clinical specimens) were indistinguishable by Penner serotyping, antimicrobial susceptibility testing, and pulsedfield gel electrophoresis. These ...
The enteric pathogen, Campylobacter jejuni, produces a range of LOS structures, however, the precise functions of LOS molecules in infection are largely undetermined. LOS structural diversity is known to arise from variation in LOS biosynthesis gene content and gene sequence. In determining the extent of LOS biosynthesis gene content variation in a group of mainly clinical C. jejuni isolates, in this study two new clusters of LOS biosynthesis genes have been identified. The C. jejuni LOS core can also undergo phase variation due to the presence of GC homopolymeric tracts in the protein coding sequence of biosynthesis genes in the cluster. Therefore, the variation in homopolymeric tract length was investigated in five genes including those in the LOS biosynthesis cluster. Many bacteria are known to vary LPS or LOS structure in response to different environment stimuli. Following the identification of a number of promoters in the LOS core biosynthesis cluster, promoter activity was measured ...
Campylobacter jejuni is the leading bacterial cause of gastrointestinal diarrheal disease in humans worldwide. This zoonotic pathogen has a complex epidemiology due to its presence in many different host organisms. The overall aim of this thesis was to explore the role of amoebae of the genus Acanthamoeba as an intermediate host and vector for survival and dissemination of C. jejuni. Earlier studies have shown that C. jejuni can enter, survive and replicate within Acanthamoebae spp. In this thesis, I have shown that C. jejuni actively invades Acanthamoeba polyphaga. Once inside, C. jejuni could survive within the amoebae by avoiding localization to degradative lysosomes. We also found that A. polyphaga could protect C. jejuni in acid environments with pH levels far below the range in which the bacterium normally survives. Furthermore, low pH triggered C. jejuni motility and invasion of A. polyphaga. In an applied study I found that A. polyphaga also could increase the survival of C. jejuni in ...
Background: Guillain-Barré syndrome (GBS) is a post-infectious polyradiculoneuropathy, frequently associated with antecedent Campylobacter jejuni (C. jejuni) infection. The presence of sialic acid on C. jejuni lipo-oligosaccharide (LOS) is considered a risk factor for development of GBS as it crucially determines the structural homology between LOS and gangliosides, explaining the induction of cross-reactive neurotoxic antibodies. Sialylated C. jejuni are recognised by TLR4 and sialoadhesin; however, the functional implications of these interactions in vivo are unknown.. Methodology/Principal Findings: In this study we investigated the effects of bacterial sialylation on phagocytosis and cytokine secretion by mouse myeloid cells in vitro and in vivo. Using fluorescently labelled GM1a/GD1a ganglioside-mimicking C. jejuni strains and corresponding (Cst-II-mutant) control strains lacking sialic acid, we show that sialylated C. jejuni was more efficiently phagocytosed in vitro by BM-MΦ, but not by ...
The food-borne bacterial pathogen Campylobacter jejuni efficiently utilizes organic acids such as lactate and formate for energy production. Formate is rapidly metabolized via the activity of the multisubunit formate dehydrogenase (FDH) enzyme, of which the FdhA subunit is predicted to contain a selenocysteine (SeC) amino acid. In this study we investigated the function of the cj1500 and cj1501 genes of C. jejuni, demonstrate that they are involved in selenium-controlled production of FDH, and propose the names fdhT and fdhU, respectively. Insertional inactivation of fdhT or fdhU in C. jejuni resulted in the absence of FdhA and FdhB protein expression, reduced fdhABC RNA levels, the absence of FDH enzyme activity, and the lack of formate utilization, as assessed by 1H nuclear magnetic resonance. The fdhABC genes are transcribed from a single promoter located two genes upstream of fdhA, and the decrease in fdhABC RNA levels in the fdhU mutant is mediated at the posttranscriptional level. FDH ...
The highly variable flagellin-encoding flaA gene has long been used for genotyping Campylobacter jejuni and Campylobacter coli. High-resolution melting (HRM) analysis is emerging as an efficient and robust method for discriminating DNA sequence variants. The objective of this study was to apply HRM analysis to flaA-based genotyping. The initial aim was to identify a suitable flaA fragment. It was found that the PCR primers commonly used to amplify the flaA short variable repeat (SVR) yielded a mixed PCR product unsuitable for HRM analysis. However, a PCR primer set composed of the upstream primer used to amplify the fragment used for flaA restriction fragment length polymorphism (RFLP) analysis and the downstream primer used for flaA SVR amplification generated a very pure PCR product, and this primer set was used for the remainder of the study. Eighty-seven C. jejuni and 15 C. coli isolates were analyzed by flaA HRM and also partial flaA sequencing. There were 47 flaA sequence variants, and all ...
Purpose and methodology. Campylobacter jejuni is a major zoonotic pathogen that causes food-borne gastroenteritis worldwide. However, there are only a few studies available that have molecularly characterized C. jejuni strains isolated in Brazil. The aim of this study was to genotype 111 C . jejuni strains isolated from sick humans (43), monkey faeces (19), chicken faeces (14), chicken meat (33) and sewage (2) between 1996 and 2016 in Brazil using flaA-SVR (short variable region) sequencing and PFGE. Furthermore, the presence of 16 virulence genes was analysed by PCR. Results. Using PFGE and flaA-SVR sequencing, the 111 C. jejuni strains studied were grouped into three and two clusters, respectively, and some strains of different origin presented a similarity of ≥80 %. In total, 35 flaA-SVR alleles were detected. Alleles gt45, gt49 and gt57 were the most prevalent, in contrast with those frequently described in the PubMLST database. All 111 C . jejuni strains contained the genes flaA, flhA, cadF, docA
TY - JOUR. T1 - AI-2 does not function as a quorum sensing molecule in Campylobacter jejuni during exponential growth in vitro. AU - Holmes, K.. AU - Tavender, T.J.. AU - Winzer, K.. AU - Wells, J.. AU - Hardie, K.. PY - 2009. Y1 - 2009. N2 - Background - Campylobacter jejuni contains a homologue of the luxS gene shown to be responsible for the production of the signalling molecule autoinducer-2 (AI-2) in Vibrio harveyi and Vibrio cholerae. The aim of this study was to determine whether AI-2 acted as a diffusible quorum sensing signal controlling C. jejuni gene expression when it is produced at high levels during mid exponential growth phase. Results - AI-2 activity was produced by the parental strain NCTC 11168 when grown in rich Mueller-Hinton broth (MHB) as expected, but interestingly was not present in defined Modified Eagles Medium (MEM-a). Consistent with previous studies, the luxS mutant showed comparable growth rates to the parental strain and exhibited decreased motility halos in both ...
The use of antimicrobials in human and veterinary medicine has coincided with a rise in antimicrobial resistance (AMR) in the food-borne pathogens Campylobacter jejuni and Campylobacter coli. Faecal contamination from the main reservoir hosts (livestock, especially poultry) is the principal route of …
Bacterial populations can display high levels of genetic structuring but the forces that influence this are incompletely understood. Here, by combining modelling approaches with multilocus sequence data for the zoonotic pathogen Campylobacter, we investigated how ecological factors such as niche (host) separation relate to population structure. We analysed seven housekeeping genes from published C. jejuni and C. coli isolate collections from a range of food and wild animal sources as well as abiotic environments. By reconstructing genetic structure and the patterns of ancestry, we quantified C. jejuni host association, inferred ancestral populations, investigated genetic admixture in different hosts and determined the host origin of recombinant C. jejuni alleles found in hybrid C. coli lineages. Phylogenetically distinct C. jejuni lineages were associated with phylogenetically distinct wild birds. However, in the farm environment, phylogenetically distant host animals shared several C. jejuni lineages
Campylobacter jejuni is the most common cause of bacterial gastroenteritis, and while typically self-limiting, C. jejuni infections are associated with post-infectious intestinal disorders, including inflammatory bowel disease and irritable bowel syndrome. This study sought to determine if C. jejuni induces virulence in commensal, non-invasive E. coli. Expression of adhesin, flagella, hemolysin, biofilm formation, and antibiotic resistance genes were increased in E. coli upon exposure to C. jejuni-conditioned media. Atomic force microscopy demonstrated E. coli was more adherent to human colonic epithelial cells when exposed to C. jejuni-conditioned media. C. jejuni and C. jejuni-conditioned media also induced E. coli flagella expression. In vitro, this altered E. coli phenotype disrupted TLR4 expression and induced IL-8 secretion. These data suggest C. jejuni and C. jejuni-conditioned media can induce virulence in non-invasive, commensal E. coli, and this contributes to host inflammation. These ...
Campylobacter jejuni, a gram-negative bacterium, is a frequent cause of gastrointestinal food-borne illness in humans throughout the world. There are several reports that the virulence of C. jejuni might be modulated by non-flagellar proteins that are secreted through the filament. Recently, FspA (Flagella secreted proteins) have been described. Two alleles of fspA (fspA1 and fspA2) based on sequence analysis were previously reported and only the fspA2 allele was found in Thai isolates. The aim of this study is to analyze the deduced amino acid sequences fspA and the adjacent putative integral membrane protein from 103 Thai C. jejuni isolates. A total of 103 representative C. jejuni isolates were amplified by PCR for the fspA gene and the adjacent integral membrane protein gene. Two PCR product sizes were amplified using the same primers, an approximately 1600-bp PCR product from 19 strains that contained fspA and integral membrane protein genes and an approximately 800-bp PCR product from 84 strains
Campylobacter jejuni is a leading cause of foodborne illnesses worldwide. Its porA gene encodes the major outer membrane protein (MOMP) that is abundantly expressed and has important physiological functions, including a key role in systemic infection and abortion induction in pregnant animals. Despite the importance of porA in... ...
Campylobacter jejuni infection produces a spectrum of clinical presentations in humans - including asymptomatic carriage, watery diarrhea, and bloody diarrhea - and has been epidemiologically associated with subsequent autoimmune neuropathies. This microorganism is genetically variable and possesses genetic mechanisms that may contribute to variability in nature. However, relationships between genetic variation in the pathogen and variation in disease manifestation in the host are not understood. We took a comparative experimental approach to explore differences among different C. jejuni strains and studied the effect of diet on disease manifestation in an interleukin-10 deficient mouse model. In the comparative study, C57BL/6 interleukin-10-/- mice were infected with seven genetically distinct C. jejuni strains. Four strains colonized the mice and caused disease; one colonized with no disease; two did not colonize. A DNA:DNA microarray comparison of the strain that colonized mice without disease to C.
Waterborne Campylobacter jejuni outbreaks are common in the Nordic countries, and PFGE (pulsed field gel electrophoresis) remains the genotyping method of choice in outbreak investigations. However, PFGE cannot assess the clonal relationship between isolates, leading to difficulties in molecular epidemiological investigations. Here, we explored the applicability of whole genome sequencing to outbreak investigation by re-analysing three C. jejuni strains (one isolated from water and two from patients) from an earlier resolved Finnish waterborne outbreak from the year 2000. One of the patient strains had the same PFGE profile, as well as an identical overall gene synteny and three polymorphisms in comparison with the water strain. However, the other patient isolate, which showed only minor differences in the PFGE pattern relative to the water strain, harboured several polymorphisms as well as rearrangements in the integrated element CJIE2. We reconstructed the genealogy of these strains with ClonalFrame
Previous studies on Campylobacter jejuni have demonstrated the role of LuxS in motility, cytolethal distending toxin production, agglutination, and intestinal colonization; however, its direct involvement in virulence has not been reported. In this study, we demonstrate a direct role of luxS in the virulence of C. jejuni in two different animal hosts. The IA3902 strain, a highly virulent sheep abortion strain recently described by our laboratory, along with its isogenic luxS mutant and luxScomplement strains, was inoculated by the oral route into both a pregnant guinea pig virulence model and a chicken colonization model. In both cases, the IA3902luxS mutant demonstrated a complete loss of ability to colonize the intestinal tract. In the pregnant model, the mutant also failed to induce abortion, while the wild-type strain was highly abortifacient. Genetic complementation of the luxSgene fully restored the virulent phenotype in both models. Interestingly, when the organism was inoculated into guinea pigs
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Cattle play a significant role in C. jejuni epidemiology as an important host to campylobacter strains that are capable of causing disease in humans (31). Based on data sets comprised of sample collections of C. jejuni isolates from disparate animal sources, a number of MLST studies have provided a growing body of evidence for host specificity among C. jejuni genotypes, including distinct pathogenic isolates associated with cattle (5, 8, 12, 19). Here, we present findings from the first longitudinal study and the largest to date MLST survey with respect to C. jejuni populations in cattle, based on a set of epidemiologically linked isolates from dairy cattle farms within a defined geographical region over a temporally continuous period, with the primary objective of investigating the importance of cattle as a reservoir for human campylobacter infections.. Depending on a range of factors, including sampling type and size, recovery methods, herd type, season, and geography, wide discrepancies in ...
Bacterial adherence to host epithelial cells is proposed to be critical for chicken colonization, as cell attachment may prevent clearance of the bacteria via host-mediated mechanical force. The goal of this study was to assess the conservation of the putative C. jejuni adhesin-encoding genes cadF, capA, jlpA, peb1A, porA, Cj1279c (flpA), and Cj1349c and the contribution of the corresponding proteins in C. jejuni host cell interactions. In this study, we found that the cadF, jlpA, porA, peb1A, flpA, and Cj1349c genes were conserved among the isolates, whereas the presence of the capA gene was variable. We found that the C. jejuni CadF, CapA, FlpA, and Cj1349c proteins contribute to the bacteriums in vitro adherence to chicken LMH hepatocellular carcinoma epithelial cells, while CadF, PEB1, and FlpA contribute to the bacteriums in vivo colonization of broiler chicks. This is the first study to show that FlpA promotes the binding of C. jejuni to host cells and plays a role in C. jejuni ...
Campylobacter jejuni is a major cause of bacterial gastroenteritis worldwide, primarily associated with the consumption of contaminated poultry. C. jejuni lineages vary in host range and prevalence in human infection, suggesting differences in survival throughout the poultry processing chain. From 7,343 MLST-characterised isolates, we sequenced 600 C. jejuni and C. coli isolates from various stages of poultry processing and clinical cases. A genome-wide association study (GWAS) in C. jejuni ST-21 and ST-45 complexes identified genetic elements over-represented in clinical isolates that increased in frequency throughout the poultry processing chain. Disease-associated SNPs were distinct in these complexes, sometimes organised in haplotype blocks. The function of genes containing associated elements was investigated, demonstrating roles for cj1377c in formate metabolism, nuoK in aerobic survival and oxidative respiration, and cj1368-70 in nucleotide salvage. This work demonstrates the utility of GWAS for
The speed that bacterial pathogens gain resistance to antibiotics is alarming. Designing new antibacterial agents is urgent, but it requires understanding their bacterial targets at the molecular level to achieve high specificity and potency. In this thesis, I discuss the structural and biochemical investigations of three potential protein targets for antibiotics. The first is a UDP-Glc/GlcNAc 4-epimerase, called Gne, from the human pathogen Campylobacter jejuni. This enzyme is the sole source of N-acetylgalactosamine (GalNAc) in C. jejuni, which is a common component in three major glycoconjugates decorating the cell surface and is critical for pathogenesis. The second target protein is an integral membrane protein, called MraY, which catalyzes the transfer of phospho-N-acetylmuramyl (MurNAc) pentapeptide to a lipid carrier, undecaprenyl phosphate (C55-P), producing Lipid I in the peptidoglycan biosynthesis pathway. In the following step, a peripheral protein called MurG catalyzes transferring ...
Campylobacter jejuni is a major bacterial cause of food-borne enteritis, and its lipooligosaccharide (LOS) plays an initiating role in the development of the autoimmune neuropathy, Guillain-Barré syndrome, by induction of anti-neural cross-reactive antibodies through ganglioside molecular mimicry. Herein we describe the existence and heterogeneity of multiple LOS forms in C. jejuni strains of human and chicken origin grown at 37°C and 42°C. The C. jejuni NCTC 11168 original isolate (11168-O) was compared to the genome-sequenced variant (11168-GS), and both were found to have a lower-Mr LOS form, which was different in size and structure to the previously characterized higher-Mr form bearing GM1 mimicry. The lower-Mr form production was found to be dependent on the growth temperature as the production of this form increased from ~5 %, observed at 37°C to ~35 % at 42°C. The structure of the lower-Mr form contained a Galβ1,3GalNAc disaccharide moiety which is consistent with the termini of ...
Fitzgerald, C. and Jones, Keith and Fields, P. (2001) Evidence for inter-species recombination of the flagellin (flaA) locus between Campylobacter coli and Campylobacter jejuni. International Journal of Medical Microbiology, 291 (2). pp. 66-67. ISSN 1438-4221. Full text not available from this repository ...
Antibiotic resistance in campylobacter is an emerging global public health problem after MRSA and VRE. Fluoroquinolone and macrolide resistance have been found to be more common in this world leading foodborne pathogen. A total of fifty-six isolates of Campylobacter jejuni obtained from raw vegetables which are consumed as ulam (salad) in Malaysia, were tested with 12 antibiotics used clinically and agriculturally. The resistance was determined using the disk diffusion method. Results were determined by hierarchic numerical methods to cluster strains and antibiotics according to similarity profiles. Fifty five C. jejuni isolates from different isolation sites were all clustered together into ten groups. This indicates that the commodities (raw salad vegetables/ulam) where the isolates originated might share a similar source of cross-contamination along the production route. All antibiotics tested correlated and there were four groupings reflecting their mode of actions. Generally, C. jejuni ...
Though the incidence of Campylobacter infections continues to increase in England and Wales, microbiological evidence of sources of infection based on comparing isolates from patients and suspected sources is difficult to obtain. In addition, coinfection with more than one strain (12, 24, 25) and lack of widespread application of subtyping of Campylobacter species means that the source of a very large proportion of infections remains undetermined. In this study, a standardized genome sequence-based FAFLP was applied to C. jejuni and C. coli strains from humans, food animal hosts, and foods of animal origin to investigate the possibility that host-specific genotypes or anonymous genetic markers of host association could be detected and characterized. FAFLP was also compared with the HS serotype and phage type to determine the degree of congruence between these geno- and phenotypic methods.. In this study, two C. jejuni and 13 C. coli species-specific marker AFs were found, which may correlate ...
Studies that investigate arsenic resistance in the foodborne bacterium Campylobacter are limited. A total of 552 Campylobacter isolates (281 Campylobacter jejuni and 271 Campylobacter coli) isolated from retail meat samples were subjected to arsenic resistance profiling using the following arsenic compounds: arsanilic acid (4-2,048 μg/mL), roxarsone (4-2048 μg/mL), arsenate (16-8,192 μg/mL) and arsenite (4-2,048 μg/mL). A total of 223 of these isolates (114 Campylobacter jejuni and 109 Campylobacter coli) were further analyzed for the presence of five arsenic resistance genes (arsP, arsR, arsC, acr3, and arsB) by PCR. Most of the 552 Campylobacter isolates were able to survive at higher concentrations of arsanilic acid (512-2,048 μg/mL), roxarsone (512-2,048 μg/mL), and arsenate (128-1,024 μg/mL), but at lower concentrations for arsenite (4-16 μg/mL). Ninety seven percent of the isolates tested by PCR showed the presence of arsP and arsR genes. While 95% of the Campylobacter coli isolates
TY - JOUR. T1 - The intestinal microbiota influences Campylobacter jejuni colonization and extraintestinal dissemination in mice. AU - OLoughlin, Jason L.. AU - Samuelson, Derrick R.. AU - Braundmeier-Fleming, Andrea G.. AU - White, Bryan A.. AU - Haldorson, Gary J.. AU - Stone, Jennifer B.. AU - Lessmann, Jeremy J.. AU - Eucker, Tyson P.. AU - Konkel, Michael E.. N1 - Publisher Copyright: © 2015, American Society for Microbiology. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.. PY - 2015. Y1 - 2015. N2 - Campylobacter jejuni is a leading cause of human foodborne gastroenteritis worldwide. The interactions between this pathogen and the intestinal microbiome within a host are of interest as endogenous intestinal microbiota mediates a form of resistance to the pathogen. This resistance, termed colonization resistance, is the ability of commensal microbiota to prevent colonization by exogenous pathogens or opportunistic commensals. Although mice normally demonstrate colonization ...
The Gram-negative bacterium Campylobacter jejuni is able to enter, survive and multiply within the free living amoeba Acanthamoeba polyphaga, but the molecular mechanisms behind these events are still unclear. We have studied the uptake and intracellular trafficking of viable and heat killed bacterial cells of the C. jejuni strain 81-176 in A. polyphaga. We found that viable bacteria associated with a substantially higher proportion of Acanthamoeba trophozoites than heat killed bacteria. Furthermore, the kinetics of internalization, the total number of internalized bacteria as well as the intracellular localization of internalized C. jejuni were dramatically influenced by bacterial viability. Viable bacteria were internalized at a high rate already after 1 h of co-incubation and were observed in small vacuoles tightly surrounding the bacteria. In contrast, internalization of heat killed C. jejuni was low at early time points and did not peak until 96 h. These cells were gathered in large ...
TY - JOUR. T1 - Characterization of two putative cytochrome c peroxidases of Campylobacter jejuni involved in promoting commensal colonization of poultry. AU - Bingham-Ramos, Lacey K.. AU - Hendrixson, David R.. PY - 2008/3. Y1 - 2008/3. N2 - Campylobacter jejuni is a leading cause of bacterial gastroenteritis in humans throughout the world, but infection of animals, especially poultry, results in a commensal colonization of the intestines. We previously found that a mutant lacking docA, which encodes a putative cytochrome c peroxidase (CCP), demonstrates up to a 105-fold reduction in colonization of the chick cecum compared to wild-type C. jejuni strain 81-176. Predictions from genomic sequences identified CJJ0382 as a second locus in C. jejuni encoding a CCP, making the bacterium unusual in having two putative CCPs. To understand what advantages are imparted by having two putative CCPs, we compared the colonization requirements of C. jejuni mutants lacking DocA or Cjj0382. Unlike the ΔdocA ...
TY - JOUR. T1 - The Effects of Temperature and Innate Immunity on Transmission of Campylobacter jejuni (Campylobacterales: Campylobacteraceae) Between Life Stages of Musca domestica (Diptera: Muscidae). AU - Bahrndorff, Simon. AU - Gill, C.. AU - Lowenberger, C.. AU - Skovgard, H.. AU - Hald, Birthe. PY - 2014. Y1 - 2014. N2 - The house fly (Musca domestica L.) is a well-established vector of human pathogens, including Campylobacter spp., which can cause infection of broiler chicken flocks, and through contaminated broiler meat can cause outbreaks of campylobacteriosis in humans. We investigated whether Campylobacter jejuni (Jones) could be transferred between life stages of M. domestica (larvae-pupae-adults) and determined bacterial counts of C. jejuni at different time points after bacterial exposure. C. jejuni was transmitted from infected larvae to pupae, but not to the adult stage. Infected larvae maintained at 25 degrees C had mean bacterial numbers of 6.5 +/- 0.2 SE log(10) (colony ...
On June 29, 2011, the Wyoming Department of Health was notified of two laboratory-confirmed cases of Campylobacter jejuni enteritis among persons working at a local sheep ranch. During June, two men had reported onset of symptoms compatible with campylobacteriosis. Both patients had diarrhea, and one also had abdominal cramps, fever, nausea, and vomiting. One patient was hospitalized for 1 day. Both patients recovered without sequelae. During June, both patients had participated in a multiday event to castrate and dock tails of 1,600 lambs. Both men reported having used their teeth to castrate some of the lambs. Among the 12 persons who participated in the event, the patients are the only two known to have used their teeth to castrate lambs. During the multiday event, a few lambs reportedly had a mild diarrheal illness. Neither patient with laboratory-confirmed illness reported consumption of poultry or unpasteurized dairy products, which are common sources of exposure to C. jejuni (1). The ...
Background: Campylobacter jejuni is the main cause of gastroenteritis worldwide. Symptoms of C. jejuni infections range from asymptomatic to bloody diarrhea. Previous studies showed a strong association between diversity in presence of bacterial virulence factors including: Campylobacter invasion protein B (CiaB), fibronectin-binding outer membrane protein (CadF), cytolethal distending toxin B (CdtB), Clp ATP dependent protease (ClpP) and HtrB protein. The aim of this project was to study the association between prevalence of the aforementioned genes, the antimicrobial resistance patterns of the isolates, and the demographic data of patients. Methodology: A total of 174 archived isolates of C. jejuni were obtained from stool samples of symptomatic patients in the Microbiology Laboratory at Hamad Medical Corporation (HMC). Isolates were revived using selective media and DNA was extracted from posititve colonies. C. jejuni isolates were screened by real-time PCR for the presence of five of ...
Campylobacter spp. particularly C. jejuni has been recognized as one of the most prevalent causes of foodborne bacterial illnesses in humans. Most previous studies have focused on the transmission routes of C. jejuni from commercial flock farms to the final retail product. To date, no in vivo studies have addressed the efficacy of sulfadimethoxine in the control of C. jejuni in poultry. This dissertation research proceeds along two lines of investigation. The objectives of the first line of investigation are to determine the enumeration of Campylobacter spp. and the prevalence on both Campylobacter spp. and C. jejuni on live egg shells, to detect the presence and extent of Campylobacter spp. and C. jejuni in live birds raised in battery-cage and cage-free systems and to determine to what extent these bacteria are present in drinking water, feed, enclosures and troughs. The objectives of the second line of investigation are to determine the effects of sulfadimethoxine antibiotic on the enumeration of
Description of disease Bacterial gastroenteritis. Treatment Bacterial gastroenteritis. Symptoms and causes Bacterial gastroenteritis Prophylaxis Bacterial gastroenteritis
Molecular mimicry of Campylobacter jejuni lipo-oligosaccharides (LOS) with gangliosides in nervous tissue is considered to induce cross-reactive antibodies that lead to Guillain-Barre syndrome (GBS), an acute polyneuropathy. To determine whether specific bacterial genes are crucial for the biosynthe …
Campylobacter lanienae is a species of Campylobacter found in humans and other animals. Like other Campylobacter species, it is rod-shaped, non-glucose-fermenting, oxidase- and catalase-positive, Gram-negative and motile. Logan, J.; Burnens, A.; Linton, D.; Lawson, A. J.; Stanley, J. (2000). Campylobacter lanienae sp. nov., a new species isolated from workers in an abattoir. International Journal of Systematic and Evolutionary Microbiology. 50 (2): 865-872. doi:10.1099/00207713-50-2-865. ISSN 1466-5026. PMID 10758898. Lund, M.; Nordentoft, S.; Pedersen, K.; Madsen, M. (2004). Detection of Campylobacter spp. in Chicken Fecal Samples by Real-Time PCR. Journal of Clinical Microbiology. 42 (11): 5125-5132. doi:10.1128/JCM.42.11.5125-5132.2004. ISSN 0095-1137. Inglis GD, Kalischuk LD, Busz HW, Kastelic JP (September 2005). Colonization of cattle intestines by Campylobacter jejuni and Campylobacter lanienae. Applied and Environmental Microbiology. 71 (9): 5145-53. ...
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We report a case of neonatal Campylobacter jejuni sepsis in a 3-week-old infant who acquired the infection through transmission from a recently acquired household puppy. Genotyping of Campylobacter strains obtained from puppy and child resulted in highly homogenous findings. This represents the first genetically proven C. jejuni dog-human transmission.. ...
Kampilobakterioza je zoonoza uzrokovana rodom Campylobacter spp i vodeći je uročnik akutnog gastroenteritisa. U 80% infekcija za zarazu je odgovoran Campylobacter jejuni, u 10% zaraza je Campylobacter coli te u 0,1% Campylobacter lari. Jedini soj koji je dokazano nepatogen za čovjeka je Campylobacter jejuni suspp. doylei. Bakterija se prenosi najčešće putem hrane (goveda, svinje,perad), neklorirane vode te nepasteriziranog mlijeka. Uzrokuje infekciju kojoj su simptomi glavobolja, proljev, mučnina i grčevi koje u nekim slučajevima prati vrućica. Uspoređujući podatke sa zavoda za javno zdravstvo dviju županija, Primorsko-goranska županija i Grad Zagreb, ustanovljena je veća stopa incidencije u Primorsko-goranskoj županiji. Kampilobakterioza se češće javlja u ljetnim mjesecima dok se u prvom tromjesečju zabilježava niži broj slučajeva. U Republici Hrvatskoj od kampilobakterioze češće obolijevaju pripadnici muškog spola. U Primorsko-goranskoj županiji u razdoblju od ...
Evidence of udder excretion of Campylobacter jejuni as the cause of milk-borne campylobacter outbreak - Volume 94 Issue 2 - D. N. Hutchinson, F. J. Bolton, P. M. Hinchliffe, H. C. Dawkins, S. D. Horsley, E. G. Jessop, P. A. Robertshaw, D. E. Counter
A carne de frango consiste em uma matriz ideal para a multiplicação de micro- organismos, e, portanto, é associada a numerosas infecções em humanos. Entre as bactérias que mais geram infecções intestinais, a partir do consumo de produtos avícolas, encontra-se a espécie Campylobacter jejuni. O uso indiscriminado de antibióticos na produção animal, pode contribuir na seleção de bactérias resistentes, que podem ser disseminadas durante a produção ou processamento dos alimentos. Desta forma, são necessárias novas alternativas para o controle desse patógeno, como a utilização de bacteriófagos. O objetivo deste trabalho foi isolar, caracterizar e avaliar o efeito de um coquetel de bacteriófagos de forma individual ou associado ao antibiótico enrofloxacina, no controle de C. jejuni, em frangos de corte. Foram isolados seis bacteriófagos (BC2, BC7, BC10, BC14, BC18 e BC19) a partir de fezes de frango coletadas em duas granjas avícolas na região de Viçosa, no Estado de Minas ...
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Linton, D, Karlyshev, A V, Hitchen, P G, Morris, H R, Dell, A, Gregson, N A and Wren, B W (2000) Multiple N-acetyl neuraminic acid synthetase (neuB) genes in Campylobacter jejuni: identification and characterization of the gene involved in sialylation of lipo-oligosaccharide. Molecular microbiology, 35(5), pp. 1120-1134. ISSN (print) 0950-382X ...
TY - JOUR. T1 - Jejunal Infection With Campylobacter. AU - Ward, Thomas T.. AU - Klein, Kenneth. AU - Borthistle, Bruce K.. PY - 1984/5. Y1 - 1984/5. N2 - A patient had common variable immunodeficiency, chronic malabsorption, and Campylobacter jejuni infection. Infection was diagnosed by jejunal aspiration. A follow-up jejunal aspirate was culture positive at the same time that a stool culture was negative. Infection resulted in worsening of chronic diarrhea, but it was not associated with clinical features of colitis or proctitis. The duration of infection was prolonged and initial antimicrobial therapy was ineffective. Single drug therapy with erythromycin ethylsuccinate and then chloramphenicol led to the emergence of resistant organisms. After five months of bacterial excretion, combination therapy with metronidazole and neomycin sulfate eliminated the organism. This case emphasizes that the clinical manifestations and response to therapy of C jejuni infection can be altered in ...
The Campylobacter jejuni capsular polysaccharide is important for virulence and often contains a modified heptose. In strain ATCC 700819 (a.k.a. NCTC 11168), the modified heptose branches off from the capsular backbone and is directly exposed to the environment. We reported previously that the enzymes encoded by wcaG, mlghB and mlghC are involved in heptose modification. Here, we show that inactivation of any of these genes leads to production of capsule lacking modified heptose and alters the transcription of other capsule modification genes differentially. Inactivation of mlghB or mlghC, but not of wcaG, decreased susceptibility to bile salts and abrogated invasion of intestinal cells. All mutants showed increased sensitivity to serum killing, especially wcaG::cat, and had defects in colonization and persistence in chicken intestine, but did not show significant differences in adhesion, phagocytosis and intracellular survival in murine macrophages. Together, our findings suggest that the capsular
Complete chemoenzymatic synthesis of the Forssman antigen using novel glycosyltransferases identified in Campylobacter jejuni and Pasteurella multocida
Campylobacter species are recognized as the most common cause of foodborne bacterial gastroenteritis in humans. In this study nine Campylobacter strains isolated from chicken meat and pork in Hanoi, Vietnam, were characterized using molecular methods and tested for antibiotic resistance. The nine isolates (eight C. jejuni and one C. coli) were identified by multiplex PCR, and tested for the presence or absence of 29 gene loci associated with virulence, lipooligosaccharide (LOS) biosynthesis and further functions. flaA typing, multilocus sequence typing and microarray assay investigation showed a high degree of genetic diversity among these isolates. In all isolates motility genes (flaA, flaB, flhA, fliM), colonization associated genes (cadF, docB), toxin production genes (cdtA, cdtB, secD, secF), and the LOS biosynthesis gene pglB were detected. Eight gene loci (fliY, virB11, Cje1278, Cj1434c, Cj1138, Cj1438c, Cj1440c, Cj1136) could not be detected by PCR. A differing
Campylobacter jejuni is the most common cause of bacterial gastroenteritis worldwide, with contaminated chicken meat considered to represent a major source of human infection. Biosecurity measures can reduce C. jejuni shedding rates of housed chickens, but the increasing popularity of free-range and organic meat raises the question of whether the welfare benefits of extensive production are compatible with food safety. The widespread assumption that the free-range environment contaminates extensively reared chickens has not been rigorously tested. A year-long survey of 64 free-range broiler flocks reared on two sites in Oxfordshire, UK, combining high-resolution genotyping with behavioural and environmental observations revealed: (i) no evidence of colonization of succeeding flocks by the C. jejuni genotypes shed by preceding flocks, (ii) a high degree of similarity between C. jejuni genotypes from both farm sites, (iii) no association of ranging behaviour with likelihood of Campylobacter shedding, and
Campylobacter was investigated in cecal droppings, feces, and cloacal swabs of 22 flocks of 3 to 5 week-old broilers. Risk factors and the likelihood of the presence of this agent in these flocks were determined. Management practices, such as cleaning and disinfection, feeding, drinkers, and litter treatments, were assessed. Results were evaluated using Odds Ratio (OR) test, and their significance was tested by Fishers test (p,0.05). A Campylobacter prevalence of 81.8% was found in the broiler flocks (18/22), and within positive flocks, it varied between 85 and 100%. Campylobacter incidence among sample types was homogenous, being 81.8% in cecal droppings, 80.9% in feces, and 80.4% in cloacal swabs (230). Flocks fed by automatic feeding systems presented higher incidence of Campylobacter as compared to those fed by tube feeders. Litter was reused in 63.6% of the farm, and, despite the lack of statistical significance, there was higher likelihood of Campylobacter incidence when litter was ...
Sponsored by the US Poultry & Egg Association Harold E. Ford Foundation, the project included growing and monitoring of turkey breeder hens and toms to 65 weeks of age, artificial insemination and collection of fertile eggs for hatching a second-generation meat bird flock, and then monitoring these progeny (meat bird flock). Intervention assessments included washing fertile eggs with sanitiser and feeding probiotics to both breeder hens and meat bird progeny.. Monitoring results showed that Campylobacter spread rapidly and cross-contaminated turkeys throughout the grow-out house. For both Salmonella and Campylobacter, wild strains that appeared seemed to out-compete marker strains after a few weeks and persist in the flock.. The most common wild strains were Campylobacter jejuni (tetracycline-resistant), Campylobacter coli (kanamycin-resistant) and Salmonella Agona.. Pathogens were also isolated from pest vectors (flies, beetles and a rodent) in the houses, confirming the importance of proper ...
An in-depth analysis was performed on Swedish broiler producers that had delivered chickens with Campylobacter to slaughter over several years, in order to identify possible transmission routes and formulate effective measures to prevent chickens being colonized with Campylobacter. Between 2017 and 2019, 626 samples were collected at farm level and Campylobacter was isolated from 133 (21.2%). All C. jejuni and C. coli isolated from these samples were whole-genome sequenced, together with isolates from the corresponding cecum samples at slaughter (n = 256). Core genome multi-locus sequence typing (cgMLST) analysis, using schemes consisting of 1140 and 529 genes for C. jejuni and C. coli, respectively, revealed that nearby cattle, contaminated drinking water, water ponds, transport crates, and parent flocks were potential reservoirs of Campylobacter. ...
Dr. Taylors research on Campylobacter species, particularly antibiotic resistance, DNA transformation and vector construction, have advanced Campylobacter genetics and enabled others using techniques developed in her laboratory, to make major contributions to understanding Campylobacter pathogenesis. Dr. Taylor was the first to demonstrate that Campylobacter jejuni and Campylobacter coli harbored tetracycline resistance (TcR) plasmids, which were transmissible only within the Campylobacter species, but not to other species such as Escherichia coli. She has spent the past 30 years investigating the novel mechanism of TcR, initially identified on Campylobacter plasmids and called Tet(O) but now recognized as an extremely common cause of TcR. Her group coined the term ribosomal protection for this mode of resistance, determined its gene sequence and similarity to translocation factors EF-G and EF-Tu. Recently her group showed that the Tet(O) protein modifies the ribosome by changing its ...
Author SummaryCampylobacter jejuni is the major cause of bacterial food-borne illness worldwide. Predation of C. jejuni by virulent bacteriophage offers the prospect of controlling bacterial populations at source in poultry. We report that in chickens, bacteriophage resistance is infrequent because the mutants that escape bacteriophage are not proficient in poultry colonisation but readily revert back to colonisation-proficient phage-sensitive types. Bacteriophage resistance is generated by reversible genomic scale inversions, leading to the activation of an unrelated bacteriophage integrated into the bacterial genome. These data not only suggest that bacteriophage therapy of C. jejuni would remain a sustainable measure to reduce poultry contamination but also demonstrate how bacterial genomes can evolve under the strong and widespread pressure of bacteriophage predation in the environment.
Aims: In view of recent findings that a multidrug efflux pump CmeABC exists in Campylobacter jejuni, 391 C. jejuni and 52 Campylobacter coli of human and animal origin were examined for a multidrug resistance phenotype.. Materials and methods: The MICs of ampicillin, chloramphenicol, ciprofloxacin, erythromycin, kanamycin, tetracycline, cetrimide, triclosan, acridine orange, paraquat and ethidium bromide were determined. Resistance to organic solvents and the effect of salicylate (known inducer of the marRAB operon in Escherichia coli and Salmonella) were also examined.. Results: Two C. coli and 13 C. jejuni isolates, mainly from pigs or poultry, were resistant to three or more antibiotics and 12 of these strains had reduced susceptibility to acridine orange and/or ethidium bromide. Strains (n = 20) that were less susceptible to acridine orange, ethidium bromide and triclosan were significantly more resistant (P , 0.05) to ampicillin, chloramphenicol, ciprofloxacin, erythromycin, nalidixic acid ...
Campylobacter jejuni and Campylobacter coli are two zoonotic pathogens with a wide host range that differ by about 15% at the nucleotide level. Campylobacter coli is largely restricted to ducks but two lineages have invaded agriculture, namely the ST828 complex and the ST1150 complex which colonize chickens and pigs. Remarkably, both lineages have undergone an enormous adaptive introgression of up to 35% of their genome from Campylobacter jejuni. We use more than 500 ST828 genomes and a large collection of isolates from C. jejuni and non-agricultural C. coli in order to describe the ongoing pattern of introgression.. ...
Det konkrete pc-værktøj er udviklet i softwaret HUGIN, og her integreres viden om forskellige kontrolmetoders effekt og omkostninger. Værktøjet kan bruges på både besætnings- og flokniveau og integrerer betydningen af årstiden i forhold til forekomsten af campylobacter med tilstedeværelsen af for eksempel fluenet, øvrige vaccinationer og historisk viden om tidligere infektioner mod campylobacter i besætningen.. På baggrund heraf beregner værktøjet sandsynligheden for forskellige mængder af campylobacter i fjerkræflokken på slagtetidspunktet ved brug af forskellige kontrolmetoder. Efterfølgende sammenligner modellen omkostningerne ved forskellige kontrolmetoder med det forventede merafkast for slagteklare kyllinger med en lavere forekomst af campylobacter. Afhandlingen giver desuden en omfattende gennemgang af bekæmpelsesstrategier for campylobacter i hele fødevarekæden.. ...
Browse through articles from peer-reviewed research publications that focus on the Campylobacter species bacterial pathogen in food safety research.
SWISS-MODEL Repository entry for A1W165 (TRUD_CAMJJ), tRNA pseudouridine synthase D. Campylobacter jejuni subsp jejuni serotype O:23/36 (strain 81-176)
SWISS-MODEL Repository entry for A1VYZ2 (ILVC_CAMJJ), Ketol-acid reductoisomerase (NADP(+)). Campylobacter jejuni subsp jejuni serotype O:23/36 (strain 81-176)
ABSTRACT: Effects of 2‐nitro‐1‐propanol, 2‐nitroethanol, nitroethane, and 2‐nitro‐methyl‐propionate (0, 10, and 20 mM) on growth of Campylobacter jejuni were tested during culture in Bolton broth adjusted to pH 5.6, 7.0, or 8.2. The nitrocompounds were similarly tested against C. coli but at pH 8.2 only. Viable cell counts measured during incubation revealed main effects (P , 0.05) of all nitrocompounds on the survivability of C. jejuni. An effect of pH (P , 0.05) on the survivability of C. Jejuni during incubation with nitrocompounds was observed, with greater inhibition observed at pH 8.2 than at pH 5.6 or 7.0 for nitroethane, 2‐nitro‐l‐propanol, and 2‐nitroethanol, but not for 2‐nitro‐methyl‐propionate, which showed greatest inhibition at pH 5.6. Except for 2‐nitro‐methyl‐propionate, which was ineffective, all nitrocompounds elicited similar effects on C. coli. The effect of nitroethane and 2‐nitro‐l‐propanol (10 mM) on naturally occurring Campylobacter ...
Campylobacteriosis is an illness that is caused by the bacteria of the genus Campylobacter. Most of the disease is caused by the species called campylobacter jejuni. The symptoms of this disease is diarrhea, cramps and fever within 2-5 days of infection. The diarrhea can be bloody with nausea and vomiting. This bacteria is gram negative and has a spiral shape. This disease is one of the most common cause of diarrheal outbreaks in Indonesia though it usually do not cause death and seldom reported. The most commonly reported cases of campylobacteriosis are associated with the eating of raw meat or under cooked meat or from cross contamination of other foods from these items ...
Campylobacter jejuni is a leading cause of foodborne enteritis and has also been linked to the autoimmune neuropathy, Guillain Barré syndrome. IL-10−/− mice serve as C. jejuni colitis model, but a mouse model for GBS is lacking. Immune mechanisms responsible for C. jejuni mediated colitis, protection from colitis or initiation of autoimmune sequelae are also largely unknown. We demonstrate that IL-10−/− mice infected with colitogenic human isolates of C. jejuni had significantly upregulated Type1/17 but not Type2 cytokines in the colon coincident with infiltration of phagocytes, T cells and Innate Lymphoid Cells. Both ILCs and T cells participated in IFN-γ, IL-17 and IL-22 upregulation but in a time- and organ-specific manner. However, T cells were necessary for colitis. Furthermore, depleting IFN-γ, IL-17 or both significantly ameliorated colitis and drove the colonic responses towards Type2 cytokine and antibody induction. In contrast, C. jejuni GBS patient strains induced mild ...
Campylobacter Supplement (Boltons) is used with Campylobacter Enrichment Broth (Boltons) for the selective enrichment of Campylobacter spp. in food and water samples. Campylobacter Supplement (Boltons) is not intended for use in the diagnosis of disease or other conditions in humans ...
A scientist who presented a paper at the Society for General Microbiologys spring conference in Dublin is developing a vaccine that could prevent Campylobacter in chickens. The research is being conducted at Washington State University.. Professor Michael Konkel, who is leading the research, is studying maternal antibodies that are naturally passed from hens to their chicks. The researchers have found the bacterial molecules the antibodies attack and are using them as a starting point for a vaccine.. Food poisoning caused by Campylobacter is quite common. In fact, this bacteria is the second most common cause of foodborne illness. A recent study at the University of Aberdeen found that 81% of chicken livers purchased at grocery stores in the UK contain Campylobacter.. A report called Ranking the Risks found that the food and bacteria combination that causes the most foodborne illness with the largest cost burden is Campylobacter in poultry.. Researchers and scientists havent been successful at ...
Of all the virulence factors that were proposed for Campylobacter jejuni and related species to cause disease in humans, the discovery of toxin production was the most promising but led to a rather confusing and even disappointing stream of data. The discussion of whether proteinaceous exotoxins are relevant in disease remains open. One important reason for this lack of consensus is the anecdotal nature of the literature reports. To provide a basis for an unbiased opinion, this review compiles all described exotoxins, compares their reported properties, and provides a summary of animal model studies and clinical data. The toxins are divided into enterotoxins and cytotoxins and are sorted according to their biochemical properties. Since many Campylobacter toxins have been compared with toxins of other species, some key examples of the latter are also discussed. Future directions of toxin research that appear promising are defined. ...
Introduction. Campylobacter is one of most common causative agents of bacterial food and waterborne illness in humans (Sheppard et al., 2009) and is responsible for an estimated 2.4 million cases of human illness per year in the US alone (Thomas et al., 1999; Center for Disease Control and Prevention, (CDC), National Center for Emerging Zoonotic and Infectious Diseases, 2010). Campylobacter is a gram negative, non-spore forming, rod-shaped bacterium and the genus is comprised of at least 17 species. Most Campylobacter species are microaerophilic, and most pathogenic species require thermophilic conditions for growth. The most common species affecting humans is C. jejuni. However, among several other species, C. coli,C. lariand C. ureolyticus have also been implicated in human infection (Thomas et al., 1999; Allos, 2001; Koziel et al., 2012). Poultry are considered a major source of human infection; however, other livestock such as cattle, sheep and pigs have also been identified as potential ...
On the 27th May 2015 the Shared Regulatory Services Communicable Disease Team (Cardiff) identified two cases of Campylobacter (one in Cardiff and the other in the Vale of Glamorgan) that were linked to the same premises (Premises A) in Cardiff.. This triggered an immediate investigation and an Outbreak Control Team was subsequently convened, declaring a formal outbreak on 4th June 2015.. In total there were 33 cases meeting the case definition of which 11 were microbiologically confirmed as Campylobacter jejuni. No cases were hospitalised. 24 cases ate at Premises A on 17th May. Of the remaining cases, seven ate on 16th May and one on 18th May. The final case ate on 7th June.. Repeated environmental visits were undertaken and issues that could potentially lead to cross contamination were identified. Premises A voluntarily closed on 4th June to address these issues and reopened on 6th June.. Of the 33 cases, 31 participated in a case control study. These all ate between the 16th and 18th May. The ...
Here she specialised in retrovirology and protection against infection caused by the bacteria Campylobacter jejuni. After ... Protection against Campylobacter jejuni infection. london.ac.uk (PhD thesis). University of London. OCLC 940318607. Science, ...
The order Campylobacterales includes human pathogens such as Helicobacter pylori and Campylobacter jejuni. The only publicly ... "Bad Bug Book - BBB - Campylobacter jejuni". www.fda.gov. Retrieved 2016-04-03. "Helicobacter Pylori Infections: MedlinePlus". ...
However, Campylobacter jejuni has seven protofilaments. The basal body has several traits in common with some types of ...
She also edited one book in 1992 titled Campylobacter Jejuni: Current Status and Future Trends. The genus Campylobacter is a ... CS1 maint: discouraged parameter (link) Nachamkin, Irving; Blaser, Martin J.; Tompkins, Lucy S. (1992). Campylobacter Jejuni: ...
Campylobacter jejuni • Capnocytophaga ochracea • Corynebacterium xerosis • Enterobacter cloacae • Escherichia coli • ...
Campylobacter jejuni *Campylobacteriosis, Guillain-Barré syndrome. *Helicobacter pylori *Peptic ulcer, MALT lymphoma, Gastric ... Campylobacter, Yersinia, Aeromonas, and Plesiomonas spp. are less frequently found. Mechanisms of action vary: some bacteria ... where Campylobacter is more prominent.[2][3] About 10% to 20% of cases are due to norovirus.[3] Protozoa such as Giardia may ...
"A proteome-wide protein interaction map for Campylobacter jejuni". Genome Biol. 8 (7): R130. doi:10.1186/gb-2007-8-7-r130. PMC ...
Campylobacter jejuni is one of the most common sources of infectious enteritis, and the most common bacterial pathogen found in ... Peterson, Michael C. (2003-05-01). "Campylobacter jejuni enteritis associated with consumption of raw milk". Journal of ... In particular, campylobacter, shigella, salmonella and many other bacteria induce acute self-limited colitis, an inflammation ... In Germany, 90% of cases of infectious enteritis are caused by four pathogens, Norovirus, Rotavirus, Campylobacter and ...
A link to Campylobacter jejuni was suspected when a young girl was admitted to Second Teaching Hospital. She had become ill ... Relationship to Campylobacter jejuni infection and anti-glycolipid antibodies". Brain. 118 (3): 597-605. doi:10.1093/brain/ ... Investigators discovered that several of the chickens in the home displayed similar symptoms and C. jejuni was found in their ... Several of the paralysis patients were found to have antibodies to C. jejuni and anti-GD1a antibodies, suggesting a link ...
Campylobacter jejuni. Campylobacter jejuni-associated disease is more prevalent in individuals who express human leukocyte ... Campylobacter jejuni is detected in these lesions by immunostaining. Patients with this disease commonly have a monoclonal ... Immunostaining of small bowel lesions in these cases commonly detects the presence of Campylobacter jejuni and is predictive ... These lesions localizes to the duodenum, jejunum, or ilium in about 63, 17, and 8% of cases, respectively, or involve more than ...
... is a form of agar used to culture species of Brucella and Campylobacter jejuni Mangels JI, Douglas BP (October ... Reeser RJ, Medler RT, Billington SJ, Jost BH, Joens LA (2007). "Characterization of Campylobacter jejuni biofilms under defined ...
Avrain L, Vernozy-Rozand C, Kempf I (2004). "Evidence for natural horizontal transfer of tetO gene between Campylobacter jejuni ... TetO gene conferring resistance to tetracycline, between Campylobacter jejuni. Neochrome, a gene in some ferns that enhances ...
"Bickerstaff's brainstem encephalitis after an outbreak of Campylobacter jejuni enteritis". Journal of Neuroimmunology. Beltz, ...
The bacterium Campylobacter jejuni has been isolated from their beaks and cloacae so milk can become contaminated as they drink ... "Jackdaws as potential source of milk-borne Campylobacter jejuni infection". The Lancet. 335 (8698): 1160. doi:10.1016/0140-6736 ... This activity was linked to cases of Campylobacter gastroenteritis in Gateshead in northeast England and led the Department of ...
During her doctorate, she studied Mycobacterium tuberculosis and Campylobacter jejuni. In 2001, Allen-Vercoe moved to Canada, ...
Gastrointestinal campylobacteriosis is caused by Campylobacter jejuni or Campylobacter coli. Although it is a commensal in the ... Campylobacter is spread horizontally via the fecal-oral route. Campylobacter fetus can also cause venereal disease and abortion ... C. jejuni can be treated with the antibiotics erythromycin and tetracycline. The disease can be prevented with good husbandry ... Campylobacter infection can be confirmed by rising antibody titers, culture on a selective medium, or histological examination ...
"Reactive nitrogen species contribute to innate host defense against Campylobacter jejuni". Infection and Immunity. 76 (3): 986- ...
... and Campylobacter jejuni. Common viral contaminants include norovirus, sapovirus, rotavirus, enteroviruses, and hepatitis A and ...
Campylobacter jejuni genomes and related information at PATRIC, a Bioinformatics Resource Center funded by NIAID. ... Campylobacteriosis is an infection by the Campylobacter bacterium, most commonly C. jejuni. It is among the most common ... Furthermore, in the United Kingdom during 2000, Campylobacter jejuni was involved in 77.3% in all cases of laboratory confirmed ... C jejuni appears to achieve this by invading and destroying epithelial cells.[citation needed] C. jejuni can also cause a ...
... -7 is also used in binding to pathogens such as Campylobacter jejuni. This occurs in a sialic acid-dependent manner and ... immunoglobulin-like lectin 7 mediates selective recognition of sialylated glycans expressed on Campylobacter jejuni ...
McSweegan, E; Walker, R I (1986). "Identification and characterization of two Campylobacter jejuni adhesins for cellular and ... He published research on the disease-causing mechanisms of the bacteria Campylobacter jejuni and Enterotoxigenic Escherichia ...
"Identification and characterization of NeuB3 from Campylobacter jejuni as a pseudaminic acid synthase". The Journal of ...
November 2002). "N-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli". Science. 298 (5599 ... as glycosylation for stability or function have been expressed using the N-linked glycosylation system of Campylobacter jejuni ...
"N-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli". Science. 298 (5599): 1790-1793. ...
Approximately 30% of cases are provoked by Campylobacter jejuni bacteria, which cause diarrhea. A further 10% are attributable ... After a Campylobacter infection, the body produces antibodies of the IgA class; only a small proportion of people also produce ... The strain of Campylobacter involved may determine the risk of GBS; different forms of the bacteria have different ... to cytomegalovirus (CMV, HHV-5). Despite this, only very few people with Campylobacter or CMV infections develop Guillain-Barré ...
"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences". Nature. 403 (6770): 665-8 ... and food-borne pathogens such as Campylobacter jejuni, Salmonella Typhimurium and Yersinia enterocolitica. Their current ...
Its clinical and microbial features are similar to the food pathogen Campylobacter jejuni. It is associated with persistent, ... Arcobacter butzlerei is a common Campylobacter-like organism. ...
... including glycoproteins by using the N-linked glycosylation system of Campylobacter jejuni engineered into E. coli. Efforts are ... "N-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli". Science. 298 (5599): 1790-1793. ...
"Microarray transposon tracking for the mapping of conditionally essential genes in Campylobacter jejuni". Gene Essentiality. ... Stahl M, Stintzi A (June 2011). "Identification of essential genes in C. jejuni genome highlights hyper-variable plasticity ...
The diarrhea-causing bacteria Campylobacter jejuni is also a reported cause of transverse myelitis. Other associated causes ...
Campylobacter jejuni *Campylobacteriosis, Guillain-Barré syndrome. *Helicobacter pylori *Peptic ulcer, MALT lymphoma, Gastric ...
Campylobacter jejuni. *Clostridium perfringens. *Escherichia coli O104:H4. *Escherichia coli O157:H7 ...
Molecular Mechanisms and Potential Clinical Applications of Campylobacter jejuni Cytolethal Distending Toxin. In: Frontiers in ... Campylobacter upsaliensis, Campylobacter jejuni.[4] ...
Campylobacter jejuni *Campylobacteriosis, Guillain-Barré syndrome. *Helicobacter pylori *Peptic ulcer, MALT lymphoma, Gastric ...
Campylobacter Jejuni. *Enterohemorrhagic E. Coli. *Kolera. *Leptospiroz. *Listeria Monocytogenes. *Şigelloz. *Salmonella. *Tifo ...
Campylobacter jejuni *Campylobacteriosis, Guillain-Barré syndrome. *Helicobacter pylori *Peptic ulcer, MALT lymphoma, Gastric ... "Enterobacteriaceae, Vibrio, Campylobacter and Helicobacter". Archived from the original on 24 December 2008. Retrieved 2008-12- ...
Campylobacter jejuni. *Clostridium perfringens. *Escherichia coli O104:H4. *Escherichia coli O157:H7 ...
Campylobacter jejuni. *Clostridium perfringens. *Escherichia coli O104:H4. *Escherichia coli O157:H7 ...
Campylobacter jejuni. *Clostridium perfringens. *Escherichia coli O104:H4. *Escherichia coli O157:H7 ...
C. jejuni. C. lanienae. C. lari. C. mucosalis. C. mustelae. C. nitrofigilis. C. peloridis. C. pylori. C. rectus. C. showae. C. ... "Campylobacter jejuni-mediated disease pathogenesis: an update". Transactions of the Royal Society of Tropical Medicine and ... The genomes of several Campylobacter species have been sequenced, beginning with C. jejuni in 2000.[14][15] These genome ... Campylobacter (meaning "curved bacteria") is a genus of Gram-negative bacteria.[1] Campylobacter typically appear comma or s- ...
Campylobacter jejuni. *Clostridium perfringens. *Escherichia coli O104:H4. *Escherichia coli O157:H7 ...
Campylobacter jejuni. *Clostridium perfringens. *Escherichia coli O104:H4. *Escherichia coli O157:H7 ...
TetO geen, mis annab resistentsuse tetratsükliinile, Campylobacter jejuni vahel.[41]. Vaata ka[muuda , muuda lähteteksti]. * ... "Evidence for natural horizontal transfer of tetO gene between Campylobacter jejuni strains in chickens". J. Appl. Microbiol. 97 ...
Campylobacter jejuni. *Clostridium perfringens. *Escherichia coli O104:H4. *Escherichia coli O157:H7 ...
Campylobacter jejuni. *Clostridium perfringens. *Escherichia coli O104:H4. *Escherichia coli O157:H7 ...
Sa mga maunlad na bansa, ang Campylobacter jejuni ang pangunahing sanhi ng gastroenteraytis na sanhi ng bakterya at kalahati ng ... Ang mga birus (lalo na ang rotabirus) at ang mga uri ng bakterya na Escherichia coli at Campylobacter ang mga pangunahing sanhi ... Ang pinaka-karaniwang mga organismo ay ang: Campylobacter, Clostridium difficile, Clostridium perfringens, at Salmonella.[70] ... Ang reaktibong arthritis ay nangyayari sa 1% ng mga tao kasunod ng pagkakaroon ng impeksiyon sa Campylobacter na uri, at ang ...
Campylobacter jejuni (Campylobacteriosis, Guillain-Barré syndrome) · Helicobacter pylori (Peptic ulcer, MALT lymphoma) · ...
Campylobacter jejuni is one of the most common sources of infectious enteritis, and the most common bacterial pathogen found in ... Peterson, Michael C. (2003-05-01). "Campylobacter jejuni enteritis associated with consumption of raw milk". Journal of ... In particular, campylobacter, shigella, salmonella and many other bacteria induce acute self-limited colitis, an inflammation ... In Germany, 90% of cases of infectious enteritis are caused by four pathogens, Norovirus, Rotavirus, Campylobacter and ...
duodenum/jejunum/ileum). குடலழற்சி (Duodenitis, குடலழற்சி, Ileitis) - Peptic (duodenal) ulcer (Curling's ulcer) - Malabsorption ... "Campylobacter pylori, NSAIDS, and Smoking: Risk Factors for Peptic Ulcer Disease". American Journal of Gastroenterology 84 (10 ...
Campylobacter jejuni Proteobacteria filumean eta Campylobacter generoan sailkatzen den bakterio bat da, gizakiak infektatzen ... Campylobacter jejuni hesteetako mukosara atxikitzen da eta bi enterotoxina ekoizten ditu, gaitzaren sintomatologia eragiten ... Alexander C. Maue, Krystle L. Mohawk, David K. Giles et Frédéric Poly, « The Polysaccharide Capsule of Campylobacter jejuni ... Campylobacter guztien antzera bakterio Gram negatiboa da eta S-itxurako morfologia helikoidala du. Flagelo bat edo bi ditu, ...
Campylobacter jejuni. *Clostridium perfringens. *Escherichia coli O104:H4. *Escherichia coli O157:H7 ...
Campylobacter jejuni. *Clostridium perfringens. *Cronobacter. *Enterovirus. *Escherichia coli O104:H4. *Escherichia coli O157: ...
"Reactive nitrogen species contribute to innate host defense against Campylobacter jejuni". Infection and Immunity 76 (3): 986- ...
Campylobacter jejuni *Campylobacteriosis, Guillain-Barré syndrome. *Helicobacter pylori *Peptic ulcer, MALT lymphoma, Gastric ... "Campylobacter pylori, NSAIDS, and Smoking: Risk Factors for Peptic Ulcer Disease". American Journal of Gastroenterology. 84 ...
Campylobacter jejuni. *Clostridium perfringens. *Escherichia coli O104:H4. *Escherichia coli O157:H7 ...
Bakteri sané lumrah katemuin minakadi E. coli, Salmonella, Shigella, miwah Campylobacter jejuni. Virus sané lumrah katemuin ...
"High-resolution transcriptome maps reveal strain-specific regulatory features of multiple Campylobacter jejuni isolates". PLoS ...
"Campylobacter jejuni , Campylobacter Food Poisoning". www.about-campylobacter.com. Retrieved 2016-04-18. Balaban M, Hendrixson ... funded by NIAID Current research on Campylobacter jejuni at the Norwich Research Park Type strain of Campylobacter jejuni at ... Campylobacter jejuni is in a genus of bacteria that is among the most common causes of bacterial infections in humans worldwide ... C. jejuni is commonly associated with poultry, and is also commonly found in animal feces. Campylobacter is a helical-shaped, ...
Campylobacter jejuni.. Snelling WJ1, Matsuda M, Moore JE, Dooley JS.. Author information. 1. School of Biomedical Sciences, ... This review describes characteristics of the family Campylobacteraceae and traits of Campylobacter jejuni. The review then ... focuses on the worldwide problem of C. jejuni antimicrobial resistance and mechanisms of pathogenesis and virulence. ...
Campylobacter jejuni: Brief Summary provided by wikipedia EN Campylobacter jejuni (/ˈkæmpɪloʊˌbæktər dʒəˈdʒuːni/) is one of the ... "Campylobacter jejuni , Campylobacter Food Poisoning". www.about-campylobacter.com. Retrieved 2016-04-18.. ... Campylobacter jejuni (/ˈkæmpɪloʊˌbæktər dʒəˈdʒuːni/) is one of the most common causes of food poisoning in Europe and in the ... "Campylobacter". www.foodsafety.gov. Retrieved 2016-04-18.. * ^ a b c d e "Campylobacter: Questions and Answers". U.S. Centers ...
CheY-mediated modulation of Campylobacter jejuni virulence.. Yao R1, Burr DH, Guerry P. ... Four motile, non-adherent and non-invasive mutants of Campylobacter jejuni 81-176 generated by a site-specific insertional ... DNA identified which was capable of repressing adherence and invasion was a 0.8 kb fragment encoding the cheY gene of C.jejuni ...
Experimental Campylobacter jejuni infection in humans. J Infect Dis 1988;157:472-9. CrossRefexternal icon PubMedexternal icon ... Campylobacter jejuni is a common raw milk contaminant and is notoriously difficult to isolate from food products, because of ... Humphrey TJ, Beckett P. Campylobacter jejuni in dairy cows and raw milk. Epidemiol Infect 1987;98:263-9. CrossRefexternal icon ... Campylobacter jejuni Infections Associated with Raw Milk Consumption - Utah, 2014. Weekly / April 1, 2016 / 65(12);301-305 ...
Campylobacter jejuni Campylobacter jejuni is a Gram-negative, spiral-shaped bacterium. C. jejuni is often found colonising the ... Scanning electron microscopy image of Campylobacter jejuni. In brown you can see the filamentous flagella. Colouring is false. ... 1.) Campylobacter jejuni is the most common cause of food poisoning in the world. 2.) One of most important features that ... One of the key features of C. jejuni that helps it to cause disease is its ability to move towards nutrients and away from ...
Torres O.R., López-Ruano J.V., Cruz J.R., Cano F., Bartlett A. (1996) Campylobacter Jejuni Repeated Infections in Children from ... In Guatemalan children, seven percent of the C. jejuni infections result in acute dysentery2. As C. jejuni is only susceptible ... Among the many causes of diarrhoea, C. jejuni 1 is seldom reported in the routine search for pathogens due to the special ... In: Newell D.G., Ketley J.M., Feldman R.A. (eds) Campylobacters, Helicobacters, and Related Organisms. Springer, Boston, MA. * ...
jejuni ATCC ® 49943™ Designation: Strain LRA 094.06.89 TypeStrain=False Application: Quality control strain for API products ... Campylobacter jejuni subsp. jejuni (ATCC® 49943™) Strain Designations: Strain LRA 094.06.89 / Type Strain: no / Biosafety Level ... Nucleotide (GenBank) : AY621112 Campylobacter jejuni subsp. jejuni strain ATCC 49943 16S ribosomal RNA gene, partial sequence ...
C. jejuni is transmitted via the fecal-oral route; this is the first reported association of C. jejuni infection with exposure ... Notes from the Field: Campylobacter jejuni Infections Associated with Sheep Castration Wyoming, 2011. On June 29, 2011, the ... C. jejuni was isolated from two lambs; one isolate had a PFGE pattern indistinguishable from the two human isolates. ... Both patients provided stool specimens for laboratory testing; C. jejuni was isolated from each. The pulsed-field gel ...
Campylobacter jejuni was first categorized in the vibrios. "The new generic term Campylobacter (curved rod in Greek) was ... jejuni Description and Significance. Campylobacter jejuni is a Gram-negative bacteria that is curved and rod-shaped. It is most ... 2] Campylobacter jejuni is the number one cause of food-borne illness in the United States. Therefore, it is very important to ... Campylobacter jejuni. From MicrobeWiki, the student-edited microbiology resource. Revision as of 15:02, 15 July 2015 by Derek.M ...
Campylobacter jejuni strain 81-176 uptake into 407 intestinal cells and Citrobacter entry into T24 bladder cells was blocked by ... Unusual microtubule-dependent endocytosis mechanisms triggered by Campylobacter jejuni and Citrobacter freundii. T A ... Inhibitors of endosome acidification had no significant impact on intracellular survival of Campylobacter jejuni or Citrobacter ... Unusual microtubule-dependent endocytosis mechanisms triggered by Campylobacter jejuni and Citrobacter freundii ...
Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819. Mutation(s): 0 Gene Names: Cj1402c, pgk. EC: 2.7.2.3. ... Find proteins for Q9PMQ5 (Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)) ... Sterne and Campylobacter jejuni in the context of their structural homologs. They are the first PGKs from pathogenic bacteria ... Sterne (BaPGK) has been determined at 1.68 Å while the structure of PGK from Campylobacter jejuni (CjPGK) has been determined ...
Campylobacter jejuni subsp. jejuni. Mutation(s): 0 EC: 1.10.3. Find proteins for A0A0H3PBA4 (Campylobacter jejuni subsp. jejuni ... Multicopper oxidase from Campylobacter jejuni: a metallo-oxidase. *DOI: 10.2210/pdb3ZX1/pdb ... Recently, a periplasmic multicopper oxidase, encoded by Campylobacter jejuni, has been characterised and associated with copper ... Crystal Structure of the Multicopper Oxidase from the Pathogenic Bacterium Campylobacter Jejuni Cgug11284: Characterization of ...
tr,O69287,O69287_CAMJU Heat shock protein OS=Campylobacter jejuni OX=197 GN=clpB PE=3 SV=1 ...
Campylobacter jejuni, which is now recognized as a discrete species, is a gram negative, microaerophilic, thermophilic, ... 1984). Isolation ofCampylobacter jejuni from human feces. In: Campylobacter infection in man and animals. 1st ed. Butzler, J.P ... 1982) 30 years of Campylobacters: biochemical characteristics and a biotyping proposal forCampylobacter jejuni. J. Clin. ... 1981) Isolation ofCampylobacter fetus subsp.jejuni from zoo animals. J. Am. Vet. Med. Assoc. 179(11):1119-1122.Google Scholar ...
In this study, the aim was to find characteristic volatile organic compounds (VOCs) of C. jejuni in order to detect its ... For this reason, MOX sensors are a promising technology for the development of a rapid and sensitive system for C. jejuni. ... It has been found that a Campylobacter sample at T20 is characterized by a higher number of alcohol compounds that the one at ... Campylobacter spp infection affects more than 200,000 people every year in Europe and in the last four years a trend shows an ...
The C. jejuni MeOPN transferase mutant showed similar levels of colonization relative to the wild-type in chickens, but showed ... In C. jejuni, the observed serum mediated killing was determined to result primarily from activation of the classical ... C. jejuni produces over 47 different capsular structures, including a unique O-methyl phosphoramidate (MeOPN) modification ... Using MeOPN biosynthesis and transferase mutants generated in C. jejuni strain 81-176, we observed that loss of MeOPN from the ...
Campylobacter jejuni Proteobacteria filumean eta Campylobacter generoan sailkatzen den bakterio bat da, gizakiak infektatzen ... Campylobacter jejuni hesteetako mukosara atxikitzen da eta bi enterotoxina ekoizten ditu, gaitzaren sintomatologia eragiten ... Alexander C. Maue, Krystle L. Mohawk, David K. Giles et Frédéric Poly, « The Polysaccharide Capsule of Campylobacter jejuni ... Campylobacter guztien antzera bakterio Gram negatiboa da eta S-itxurako morfologia helikoidala du. Flagelo bat edo bi ditu, ...
jejuni ATCC ® 700819™ Designation: NCTC 11168 TypeStrain=False Application: Enteric and emerging infectious disease research ... Campylobacter jejuni subsp. jejuni (Jones et al.) Veron and Chatelain (ATCC® 700819D-5™) Add to ... Campylobacter jejuni subsp. jejuni (Jones et al.) Veron and Chatelain (ATCC® 700819™) Strain Designations: NCTC 11168 / Type ... Campylobacter jejuni subsp. jejuni (Jones et al.) Veron and Chatelain ATCC® 700819™ freeze-dried ...
Campylobacter Jejuni cooking information, facts and recipes. A foodborne pathogen that causes illness from undercooked poultry ...
Salmonella and campylobacter in chicken meat : meeting report  World Health Organization; Food and Agriculture Organization of ...
Campylobacter jejuni subsp. jejuni 81-176. › Campylobacter jejuni subsp. jejuni str. 81-176. › Campylobacter jejuni subsp. ... Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176). Taxonomy navigation. › Campylobacter jejuni subsp. jejuni ... Taxonomy - Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) Basket 0 ...
... efficacy of early treatment with erythromycin on the duration of fecal excretion and of diarrhea associated with Campylobacter ... jejuni, 170 patients, age 3 to 60 months, were randomly assigned in a double-blind fashion to receive either erythromycin ethyl ... Early treatment with erythromycin of Campylobacter jejuni-associated dysentery in children J Pediatr. 1986 Aug;109(2):355-60. ... Of the 30 patients with stools positive for C. jejuni, 12 were in the placebo group and 16 in the treatment group. After 2 days ...
In this study, we constructed an insertion mutant and complement of this gene in C. jejuni and examined changes in virulence. ... In this study, we constructed an insertion mutant and complement of this gene in C. jejuni and examined changes in virulence. ... In addition, we localized the Cj0371 protein at the poles of C. jejuni by fluorescence microscopy. We present data that ... In addition, we localized the Cj0371 protein at the poles of C. jejuni by fluorescence microscopy. We present data that ...
Campylobacter jejuni- a foodborn pathogen that is a leading cause of food poisoning. It is three times more common than ... Caption: Campylobacter jejuni- a foodborn pathogen that is a leading cause of food poisoning. It is three times more common ... Keywords: bacteria, bacterial, bacteriology, bacterium, campylobacter jejuni-, food poisoning, foodborn bacteria, foodborn ...
... Author(s). Hazeleger, W.C.; Ettema, B.; Rombouts, F.M.; ... In: proceedings 10th international workshop on Campylobacter, Helicobacter and related organisms : Baltimore, USA, 1999 - p. 25 ...
Mismatch repair - Campylobacter jejuni subsp. jejuni ICDCCJ07001 [ Pathway menu , Organism menu , Pathway entry , Download KGML ...
Campylobacter jejuni EC#: 2.7.7.60 IUBMB 4.6.1.12 IUBMB Gene Name(s): ispDF Cj1607 ... Structure of the Bifunctional IspDF from Campylobacter jejuni. *DOI: 10.2210/pdb1w55/pdb ...
Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis," ... "Campylobacter jejuni infection and the association with Guillain Barré syndrome," in Campylobacter, I. Nachamkin and M. J. ... and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples," Journal of Clinical ... "Epidemiology of Campylobacter jejuni infections in the United States and other industrialized nations," in Campylobacter, I. ...
Timeline for Species Campylobacter jejuni [TaxId:192222] from c.1.5.0 automated matches: *Species Campylobacter jejuni [TaxId: ... PDB entries in Species: Campylobacter jejuni [TaxId: 192222]:. *Domain(s) for 4mz1: *. Domain d4mz1a1: 4mz1 A:1-482 [240557]. ... Lineage for Species: Campylobacter jejuni [TaxId: 192222]. *Root: SCOPe 2.06 *. Class c: Alpha and beta proteins (a/b) [51349 ... Species Campylobacter jejuni [TaxId:192222] from c.1.5.0 automated matches appears in SCOPe 2.05. ...
  • Another chronic condition that may be associated with Campylobacter infection is reactive arthritis. (wikipedia.org)
  • Infection with C. jejuni usually results in enteritis, which is characterised by abdominal pain, diarrhea, fever, and malaise. (wikipedia.org)
  • Campylobacter jejuni infection causes cramping, diarrhea, abdominal pain and fever within 2 to 5 days after a person has been exposed to the organism. (medlineplus.gov)
  • Additional cases of C. jejuni infection were identified in October, and UDAF permanently revoked dairy A's permit to sell raw milk on December 1. (cdc.gov)
  • During May 9-November 6, 2014, a total of 99 cases of C. jejuni infection were identified. (cdc.gov)
  • On May 21, 2014, UPHL notified UDOH of three laboratory-confirmed cases (in patients A, B, and C) of C. jejuni infection with indistinguishable SmaI PFGE patterns (DBRS16.0196). (cdc.gov)
  • Campylobacter infection is a reportable disease in Utah, and all Campylobacter isolates undergo PFGE analysis ( 1 ). (cdc.gov)
  • A confirmed case was defined as the onset of diarrheal illness caused by C. jejuni matching the cluster PFGE pattern or confirmed Campylobacter infection on or after May 1 in a person who had consumed dairy A raw milk 1-10 days before illness onset. (cdc.gov)
  • During May 9-November 6, a total of 99 cases (59 confirmed and 40 probable) of C. jejuni infection were identified through laboratory isolates and patient interviews ( Figure 2 ). (cdc.gov)
  • this is the first reported association of C. jejuni infection with exposure during castration of lambs ( 2 ). (cdc.gov)
  • [11] Clinical evidence suggests that the site of Campylobacter infection seems to be the ileum and jejunum in the small intestines rather than in the large intestines. (kenyon.edu)
  • The early symptom of Campylobacter infection is usually abnormal abdominal pain and is then followed by bloody diarrhea. (kenyon.edu)
  • Complication of Campylobacter infection may also contribute to mortality. (kenyon.edu)
  • 1980). Campylobacter infection from foals. (springer.com)
  • 1978). Campylobacter enteritis associated with canine infection. (springer.com)
  • Campylobacter spp infection affects more than 200,000 people every year in Europe and in the last four years a trend shows an increase in campylobacteriosis. (mdpi.com)
  • Alexander C. Maue, Krystle L. Mohawk, David K. Giles et Frédéric Poly, « The Polysaccharide Capsule of Campylobacter jejuni Modulates the Host Immune Response », Infection and Immunity, vol. 81, 3 zk. (wikipedia.org)
  • Epidemiological studies, which established the relationship between GBS and antecedent C. jejuni infection, showed that one-fourth to one-third of GBS patients develop the syndrome after being infected. (pnas.org)
  • Campylobacter jejuni from patients with Guillain-Barré syndrome preferentially expresses a GD1a-like epitope," Infection and Immunity , vol. 70, no. 9, pp. 5299-5303, 2002. (hindawi.com)
  • B. Mishu and M. J. Blaser, "Role of infection due to Campylobacter jejuni in the initiation of Guillain-Barre syndrome," Clinical Infectious Diseases , vol. 17, no. 1, pp. 104-108, 1993. (hindawi.com)
  • B. M. Allos, "Association between Campylobacter infection and Guillain-Barre syndrome," Journal of Infectious Diseases , vol. 176, no. 6, pp. (hindawi.com)
  • The aim of this study was the investigation of the protective effects of curcumin against immune-induced epithelial barrier dysfunction in C. jejuni infection. (mdpi.com)
  • An increase in fluorescein (332 Da) permeability in co-cultures as well as in the germ-free IL-10 −/− mouse model after C. jejuni infection was shown. (mdpi.com)
  • The incidence of human campylobacteriosis is increasing worldwide, as well as the number of isolates resistant to fluoroquinolones which are one of the primary classes of antimicrobials used to treat Campylobacter infection in human therapy and thus considered of high public concern [ 1 ]. (hindawi.com)
  • Poultry is a natural reservoir of Campylobacter species, constituting the most important source of human infection. (hindawi.com)
  • In addition, infection with C. jejuni is the most frequent antecedent to a form of neuromuscular paralysis known as Guillain-Barré syndrome. (nih.gov)
  • Campylobacter jejuni is the most commonly reported bacterial cause of foodborne infection in the United States. (cdc.gov)
  • Adding to the human and economic costs are chronic sequelae associated with C. jejuni infection-Guillian-Barré syndrome and reactive arthritis. (cdc.gov)
  • Deaths from C . jejuni infection are rare and occur primarily in infants, the elderly, and patients with underlying illnesses (2) . (cdc.gov)
  • Guillain-Barré syndrome (GBS), a demyelating disorder resulting in acute neuromuscular paralysis, is a serious sequela of Campylobacter infection (7) . (cdc.gov)
  • Up to 40% of patients with the syndrome have evidence of recent Campylobacter infection (7) . (cdc.gov)
  • Uveal Effusion Associated With Campylobacter jejuni Infection Presenting as Bilateral Angle Closure Glaucoma. (biomedsearch.com)
  • INTRODUCTION: We present a case in which a gastrointestinal infection with Campylobacter jejuni presented as acute angle closure glaucoma secondary to the uveal effusion syndrome. (biomedsearch.com)
  • As the first line defense, intestinal epithelium activates NF-κB and secretes proinflammatory cytokine IL-8 in response to Campylobacter infection. (umd.edu)
  • C. jejuni is a commensal of the gastrointestinal tract of birds and most importantly poultry, the major source of human infection. (warwick.ac.uk)
  • Variability in the clinical expression of Campylobacter infection has been observed for many years. (asm.org)
  • Other clinical presentations of Campylobacter infection are meningitis ( 12 ), bacteremia ( 32 ), localized extraintestinal infections ( 5 ), and immunoreactive complications such as Guillain-Barré syndrome ( 17 , 24 ) and reactive arthritis ( 2 ). (asm.org)
  • However, when incubated together with various strains of Pseudomonas, Campylobacter were found to survive for much longer, in some cases over 48 hours, which would be easily long enough to cause infection. (barfblog.com)
  • Here we expand the knowledge on C. jejuni acid tolerance by looking at protozoa as a potential epidemiological pathway of infection. (diva-portal.org)
  • The symptoms of Campylobacter jejuni infection will last for 24 hours to about a week, in some cases the infection may last for more than a week also. (medical-wiki.com)
  • . Campylobacter jejuni is very sensitive to hydrochloric acid and if you are on antacid treatment you may be at an added risk to suffer from this infection. (medical-wiki.com)
  • Complications: After suffering from C. Jejuni infection you may develop an autoimmune complication which affects the nerves of the legs which causes an acute idiopathic demyelinating polyneuropathy also known as Guillian Barre syndrome. (medical-wiki.com)
  • With a Campylobacter jejuni infection, you may suffer from other conditions like hemolytic uremic syndrome and thrombotic thrombocytopenic purpura. (medical-wiki.com)
  • If you suffer from any of the symptoms of campylobacter infection it is best that you visit your doctor. (medical-wiki.com)
  • Although C. jejuni infection has been shown to promote intestinal inflammation, the impact of this bacterium on carcinogenesis has never been examined. (bmj.com)
  • C. jejuni infection has been shown to promote intestinal inflammation. (bmj.com)
  • IMPORTANCE Accurately quantifying the relative contribution of different host reservoirs to human Campylobacter infection is an ongoing challenge. (pubmedcentralcanada.ca)
  • Experimental Campylobacter jejuni infection in the chicken: an animal model of axonal Guillain-Barré syndrome. (bmj.com)
  • IMPORTANCE Direct comparative studies of C. jejuni infection of a zoonotic commensal host and a disease-susceptible host are crucial to understanding the causes of infection outcome in humans. (asm.org)
  • In humans, C. jejuni infection can result in sporadic cases or localized outbreaks of disease due to ingestion of contaminated meat, water, or unpasteurized milk and improper handling of animals ( 1 , 9 , 10 ). (asm.org)
  • Here, we show that CjeCas9 released by C. jejuni during the infection of Caco-2 human intestinal epithelial cells directly modulates Caco-2 transcriptomes during the first four hours of infection. (eur.nl)
  • While a prior report documented the transcriptome of C. jejuni phages during the carrier state life cycle, transcriptomic analysis of a lytic C. jejuni phage infection has not been reported. (bionity.com)
  • We used RNA-sequencing to profile the infection of C. jejuni NCTC 11168 by the lytic T4-like myovirus NCTC 12673. (bionity.com)
  • The enteric pathogen, Campylobacter jejuni, produces a range of LOS structures, however, the precise functions of LOS molecules in infection are largely undetermined. (le.ac.uk)
  • Nielsen, HL , Dalager-Pedersen, M & Nielsen, H 2019, ' Risk of inflammatory bowel disease after Campylobacter jejuni and Campylobacter concisus infection: a population-based cohort study ', Scandinavian Journal of Gastroenterology , vol. 54, no. 3, pp. 265-272. (aau.dk)
  • Thus, it is important to identify factors that influence the development of these sequelae during and after initial C. jejuni infection.Ferrets ( Mustela putorius furo ) have been previously used as an animal model of human campylobacteriosis, but intensive investigation into this model has yet to be performed. (tennessee.edu)
  • Thus, in this study, we non-invasively examined for disease in ferrets and determined whether there were any effects on development following infection with C. jejuni . (tennessee.edu)
  • Ferrets were effectively colonized by C. jejuni with peak fecal loads observed at day 3 post-infection, and with full resolution by day 12 post-infection. (tennessee.edu)
  • Additionally, in response to infection, the neutrophil protein calgranulin C (S100A12) was found to be increased in the feces of both ferrets and humans infected with C. jejuni , while calprotectin (another neutrophil protein) was not. (tennessee.edu)
  • These results suggest that upon infection with C. jejuni , neutrophils that are trafficked to the intestine release S100A12 and possibly calprotectin as a mechanism for inhibiting C. jejuni growth in the intestine. (tennessee.edu)
  • Shank, Janette Marie, "The Roles of Calprotectin and Calgranulin C in Campylobacter jejuni Infection. (tennessee.edu)
  • There is less evidence that Guillain-Barre syndrome may be a potential sequela of Campylobacter upsaliensis infection, however C. upsaliensis has been cultured from individuals with Guillain-Barre, indicating that a potential relationship between the two cannot be ruled out. (wikipedia.org)
  • The pulsed-field gel electrophoresis (PFGE) patterns of the isolates were indistinguishable when restricted separately by two enzymes, Sma I and Kpn I. This PFGE pattern had never been reported among 667 specimens from which C. jejuni was isolated in Wyoming and is rare in CDC's PulseNet* database, with a frequency of 0.09% (8 of 8,817). (cdc.gov)
  • 1983) In vitro sensitivity of 28 bovine isolates of Campylobacter to some commonly used antimicrobials. (springer.com)
  • The isolates were identical at the flaA locus, all having the allele 21/peptide 2 designation (http://pubmlst.org/campylobacter/flaA). (cdc.gov)
  • Our MLST analysis confirms that the C. jejuni isolates from the penguins were of a genotype common among humans with disease and among our food animals. (cdc.gov)
  • Campylobacter transmission in a Peruvian shantytown: a longitudinal study using strain typing of campylobacter isolates from chickens and humans in household clusters. (canarydatabase.org)
  • The objectives of this study were to characterize the pathogenic abilities of various C. jejuni/coli retail meat isolates, including their abilities to adhere to, invade into and transmigrate across human epithelial cells, to examine the role of NF-κB pathway in IL-8 secretion induced by Campylobacter, and to identify C. jejuni-specific adherence/invasion genes during host pathogen interaction. (umd.edu)
  • It was found that the adherence and invasiveness of total 43 Campylobacter retail meat isolates in human intestinal epithelial T84 cell model indicated that C. jejuni/coli present in retail meat were considerably diverse in their ability to adhere to and invade human epithelial cells. (umd.edu)
  • Meanwhile, eight putative virulence genes, determined by PCR, were shown to be widespread among the Campylobacter isolates. (umd.edu)
  • In summary, Campylobacter retail meat isolates exhibited wide diversity in cell culture model in the ability of adherence, invasion and transmigration. (umd.edu)
  • Background:Campylobacter jejuni, the most leading cause for bacterial gastroenteritis worldwide, shows a high genetic diversity among its isolates. (isaude.net)
  • Additionally more than half of human Campylobacter-isolates were assigned to a non-livestock clade associated with the absence of cj1321-cj1326. (isaude.net)
  • This report forms part of the project: A microbiological survey of Campylobacter contamination in fresh whole UK produced chilled chickens at retail sale (2015-2018) and presents AMR data for a subset of those Campylobacter isolates collected as part of this survey. (food.gov.uk)
  • A subset (393) of the Campylobacter isolates collected from 392 retail chicken samples was tested for AMR (there were 1,114 Campylobacter positive samples to choose from). (food.gov.uk)
  • Molecular characterization of invasive and noninvasive Campylobacter isolates from children with diarrhea and symptom-free children was performed by random amplified polymorphic DNA techniques (RAPD). (asm.org)
  • A distinct RAPD profile with a DNA band of 1.6 kb was observed significantly more frequently among invasive (63%) than among noninvasive (16%) Campylobacter isolates ( P = 0.000005). (asm.org)
  • These data indicate that most of the invasive Campylobacter strains could be differentiated from noninvasive isolates by RAPD analysis and PCR using specific primers that amplify a fragment of the iam locus. (asm.org)
  • In a study in Mexico, we found that 70% of C. jejuni and Campylobacter coli isolates from children with diarrhea were invasive, as determined by the HEp-2 cell chamber-slide monolayer method, while 83% of isolates from asymptomatic children were nonadherent and noninvasive ( 29 ). (asm.org)
  • Variability in the clinical expression and in the phenotypic traits of isolates may be related to genetic diversity of Campylobacter strains. (asm.org)
  • 2012. Tracing Campylobacter jejuni strains along the poultry meat production chain from farm to retail by pulsed-field gel electrophoresis, and the antimicrobial resistance of isolates. (thepoultrysite.com)
  • A total of 552 Campylobacter isolates (281 Campylobacter jejuni and 271 Campylobacter coli ) isolated from retail meat samples were subjected to arsenic resistance profiling using the following arsenic compounds: arsanilic acid (4-2,048 μg/mL), roxarsone (4-2048 μg/mL), arsenate (16-8,192 μg/mL) and arsenite (4-2,048 μg/mL). (mdpi.com)
  • A total of 223 of these isolates (114 Campylobacter jejuni and 109 Campylobacter coli ) were further analyzed for the presence of five arsenic resistance genes ( arsP , arsR , arsC , acr3 , and arsB ) by PCR. (mdpi.com)
  • Most of the 552 Campylobacter isolates were able to survive at higher concentrations of arsanilic acid (512-2,048 μg/mL), roxarsone (512-2,048 μg/mL), and arsenate (128-1,024 μg/mL), but at lower concentrations for arsenite (4-16 μg/mL). (mdpi.com)
  • The presence of arsC and acr3 did not significantly increase arsenic resistance with the exception of conferring resistance to higher concentrations of arsenate to some Campylobacter isolates. (mdpi.com)
  • To our knowledge, this is the first study to determine arsenic resistance and the prevalence of arsenic resistance genes in such a large number of Campylobacter isolates. (mdpi.com)
  • The isolates 15 (24,2%) that serologically reacted with Campylobacter pool 7 antiserum consist of the most prevalent serotype, followed by those that reacted with pool 8 antiserum, 12 (19,4%) and the remaining with pool 1,2,3,4,5,6 and 9 antisera were also represented. (journals.co.za)
  • Here we present a source attribution approach based on the identification of novel epidemiological markers among a reference pan-genome list of 1,810 genes identified by gene-by-gene comparison of 884 genomes of Campylobacter jejuni isolates from animal reservoirs, the environment, and clinical cases. (pubmedcentralcanada.ca)
  • Fifteen loci involved in metabolic activities, protein modification, signal transduction, and stress response or coding for hypothetical proteins were selected as host-segregating markers and used to attribute the source of 42 French and 281 United Kingdom clinical C. jejuni isolates. (pubmedcentralcanada.ca)
  • A systematic analysis using gene-by-gene comparison of 884 genomes of C. jejuni isolates, with a pan-genome list of genes, identified 15 novel epidemiological markers for source attribution. (pubmedcentralcanada.ca)
  • The different proportions of French and United Kingdom clinical isolates attributed to each host reservoir illustrate a potential role for local/national variations in C. jejuni transmission dynamics. (pubmedcentralcanada.ca)
  • Although poultry is considered as the main source of C. jejuni, many strains found in poultry (identified by various genotyping techniques) cannot be traced in the human population and in the Netherlands, of all human isolates, only 30% has been detected in poultry. (openrepository.com)
  • The duplex-PCR assay on colonies detected 8 isolates of C. jejuni and 1 isolate C. coli. (magiran.com)
  • Since antimicrobial-resistant Campylobacter can be transmitted from food animals to humans through the food chain, the rising resistance to antibiotics among Campylobacter isolates of animal origin is a concern for public health. (asm.org)
  • In determining the extent of LOS biosynthesis gene content variation in a group of mainly clinical C. jejuni isolates, in this study two new clusters of LOS biosynthesis genes have been identified. (le.ac.uk)
  • Campylobacter jejuni subsp. (atcc.org)
  • Survival of Campylobacter fetus subsp. (springer.com)
  • AL111168 Campylobacter jejuni subsp. (atcc.org)
  • Mismatch repair - Campylobacter jejuni subsp. (genome.jp)
  • it is usually found in the intestinal tract of various farm and wild animals, particularly birds ( 6 , 7 ).We previously reported finding 3 C. jejuni subsp. (cdc.gov)
  • Molecular subtyping of Campylobacter jejuni subsp. (scielo.br)
  • Subtipagem molecular de estirpes de Campylobacter jejuni subsp. (scielo.br)
  • The objective of the present trial was to characterize genetically strains of Campylobacter jejuni subsp. (scielo.br)
  • O objetivo do presente trabalho foi caracterizar geneticamente estirpes de Campylobacter jejuni subsp. (scielo.br)
  • Microbiologics : 0111L Campylobacter jejuni subsp. (microbiologics.com)
  • Microbiologics : 0251P Campylobacter jejuni subsp. (microbiologics.com)
  • Campylobacter jejuni is in a genus of bacteria that is among the most common causes of bacterial infections in humans worldwide. (wikipedia.org)
  • Campylobacteriosis is an infectious disease caused by bacteria of the genus Campylobacter. (wikipedia.org)
  • Campylobacter jejuni is a Gram-negative bacteria that is curved and rod-shaped. (kenyon.edu)
  • The Campylobacter bacteria is found in the intestines of many animals and some humans. (kenyon.edu)
  • Campylobacter jejuni is a Gram-negative bacteria like bacilli. (kenyon.edu)
  • Campylobacter jejuni is a species of curved, long rod-shaped, Gram-negative microaerophilic, bacteria commonly found in animal feces. (thermofisher.com)
  • Furthermore, the kinetics of internalization, the total number of internalized bacteria as well as the intracellular localization of internalized C. jejuni were dramatically influenced by bacterial viability. (diva-portal.org)
  • Here's one way: the bug latches onto other colonies of bacteria - biofilms - and uses them as places to thrive in ways the C. jejuni would be less likely to do on their own. (thepoultrysite.com)
  • C. jejuni has had a major disadvantage in that unlike many other bacteria, it does not do well at making its own biofilm. (thepoultrysite.com)
  • The host colonizers can be any of several bacteria, but C. jejuni 's most prevalent host turns out to be Pseudomonas, which also serve as the main spoilage bacteria on chicken carcasses and are excellent biofilm formers, Dr Hanning said. (thepoultrysite.com)
  • US - Finding how the fowl-borne bacteria Campylobacter jejuni makes at least a million Americans miserable for a week each year is on the plates of two Medical College of Georgia microbiologists. (thepoultrysite.com)
  • One of these components in C. jejuni is a proton pump called complex I, which is widely found in bacteria, archaea, and the mitochondria of eukaryotes ( 12 ). (asm.org)
  • The other 12 subunits of complex I in C. jejuni do contain sequence similarities to complex I subunits of other bacteria including E. coli and P. denitrificans ( 19 , 39 ). (asm.org)
  • Mating of the bacteria on plates or in liquid broth medium produced C. jejuni progeny containing the streptomycin marker. (asm.org)
  • In fact, it's such a bad idea that the National Health Service (NHS) in Britain issued a public warning against the "sanitary" practice - claiming that "it can increase your risk of food poisoning from campylobacter bacteria. (barfblog.com)
  • A new study from the University of Veterinary Medicine in Vienna has shown that Campylobacter jejuni is protected and dependent upon the presence of spoilage bacteria on meat, in particular Pseudomonas for survival. (barfblog.com)
  • It seems possible that the various species interact and Hilbert hypothesized that such interactions might help bacteria such as Campylobacter jejuni survive under hostile, oxygen-rich conditions. (barfblog.com)
  • She thus tested the survival of C. jejuni in the presence of various meat-spoiling bacteria. (barfblog.com)
  • When incubated alone or together with bacteria such as Proteus mirabilis or Enterococcus faecalis, Campylobacter survived atmospheric oxygen levels for no longer than 18 hours. (barfblog.com)
  • Our results showed that when C. jejuni cells were coincubated with Acanthamoeba polyphaga in acidified phosphate-buffered saline (PBS) or tap water, the bacteria could tolerate pHs far below those in their normal range, even surviving at pH 4 for 20 h and at pH 2 for 5 h. (diva-portal.org)
  • Interestingly, moderately acidic conditions (pH 4 and 5) were shown to trigger C. jejuni motility as well as to increase adhesion/internalization of bacteria into A. polyphaga. (diva-portal.org)
  • Campylobacter jejuni is a Gram negative bacteria and is commonly found in the feces of animals and in the gastrointestinal tract of many birds. (medical-wiki.com)
  • Bacteria in the Campylobacter genus are widely distributed and can be isolated from both domestic and wild animal species (1,3,20). (scielo.br)
  • The capacity for Campylobacter species to survive and thrive in a wide range of environmental niches is underpinned by the ability of the bacteria to utilise the different metabolites that are available in the various hosts and environments. (biomedcentral.com)
  • Campylobacter upsaliensis is a gram negative bacteria in the Campylobacter genus. (wikipedia.org)
  • Bacterial invasion of six different human epithelial cell lines showed that some strains of the intestinal pathogen Campylobacter jejuni invaded intestinal cell lines at a level 10(2)-10(4) times higher than reported previously for other Campylobacter strains. (pnas.org)
  • The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences. (atcc.org)
  • The Gram-negative bacterial pathogen Campylobacter jejuni is a major cause of foodborne gastroenteritis worldwide. (rsc.org)
  • The zoonotic human pathogen Campylobacter jejuni is known for its ability to induce DNA-damage and cell death pathology in humans. (eur.nl)
  • Indeed, many other genes in C. jejuni are subject to phase variation, including those responsible for sticking to host molecules and taking up essential nutrients. (le.ac.uk)
  • The crucial role of Campylobacter jejuni genes in anti-ganglioside antibody induction in Guillain-Barré syndrome," Journal of Clinical Investigation , vol. 114, no. 11, pp. 1659-1665, 2004. (hindawi.com)
  • O objetivo desse trabalho foi avaliar a diversidade genética por cinco diferentes técnicas de tipagem molecular, o potencial patogênico pela pesquisa de 16 genes de virulência por PCR e o perfil de resistência pela concentração inibitória mínima por Etest® frente a quatro antimicrobianos e pela análise in silico de genes de resistência e pontos de mutação de linhagens de C. jejuni isoladas no Brasil. (usp.br)
  • Conclui-se que a alta frequência da maioria dos genes de virulência pesquisados evidenciou o potencial patogênico das linhagens de C. jejuni estudadas. (usp.br)
  • Previously, it was thought that occasional mutations to topoisomerase IV (parC and parE) were the basis for fluoroquinolone resistance, but sequencing of the C. jejuni genome shows that it lacks those genes. (renalandurologynews.com)
  • The effort to identify C. jejuni-specific adherence/invasion genes during host pathogen interaction by using restriction fragment differential display PCR (RFDD-PCR) has been made. (umd.edu)
  • 2106), the extent of variation in 27 phase-variable genes of C. jejuni strain 11168 has been explored during in vitro passage, infections of chickens and after imposition of non-selective bottlenecks. (warwick.ac.uk)
  • Campylobacter jejuni encodes 12 of the 14 subunits that make up the respiratory enzyme NADH:ubiquinone oxidoreductase (also called complex I). The two nuo genes not present in C. jejuni encode the NADH dehydrogenase, and in their place in the operon are the novel genes designated Cj1575c and Cj1574c. (asm.org)
  • C. jejuni exposed to extracts had altered expression of 40 genes related to iron uptake, metabolism, chemotaxis, energy production, and osmotic stress response. (asm.org)
  • We identified C. jejuni genes more highly expressed during exposure to human fecal extracts in comparison to chicken cecal extracts and differentially expressed in extracts compared with medium alone, and targeted one specific iron uptake system for further molecular, genetic, and phenotypic study. (asm.org)
  • The C. jejuni LOS core can also undergo phase variation due to the presence of GC homopolymeric tracts in the protein coding sequence of biosynthesis genes in the cluster. (le.ac.uk)
  • In Campylobacter , mutations in the 23S rRNA genes and efflux, mediated by the CmeABC pump, were both shown to contribute to macrolide resistance ( 11 ). (asm.org)
  • In this study, a double-locus sequence typing (DLST) scheme for C. jejuni based on concatenated partial sequences of porA and peb1A genes is proposed. (edu.au)
  • A broader PCR screen of 16 C. coli strains and 42 C. jejuni confirmed the absence of the genes needed for propanoate metabolism. (biomedcentral.com)
  • C. jejuni is commonly associated with poultry, and is also commonly found in animal feces. (wikipedia.org)
  • Most cases of Campylobacter jejuni come from handling or ingesting raw or undercooked poultry meat. (medlineplus.gov)
  • C. jejuni is often found colonising the stomachs of poultry, where it very rarely causes any disease. (le.ac.uk)
  • This mutual relationship with poultry is one of the reasons C. jejuni is well adapted to causing disease in humans. (le.ac.uk)
  • Neither patient with laboratory-confirmed illness reported consumption of poultry or unpasteurized dairy products, which are common sources of exposure to C. jejuni ( 1 ). (cdc.gov)
  • Poultry is a major source of C. jejuni , and only a small proportion of campylobacteriosis cases can be attributed to other animals or environmental sources ( Chen and Jiang, 2014 ). (frontiersin.org)
  • C. jejuni is commonly associated with poultry and naturally colonizes the G1 tract of many bird species. (thermofisher.com)
  • This study aimed to analyse the diversity of the Campylobacter jejuni population in broilers and to evaluate the major source of contamination in poultry meat. (hindawi.com)
  • Specific Campylobacter populations could be harboured within each poultry farm, with the ability to contaminate chickens during each new cycle. (hindawi.com)
  • Campylobacter colonization in chickens takes place at poultry farms, approximately 7 days after hatching [ 13 ], while widespread carcass contamination occurs at the slaughterhouse, especially from cross contamination by intestinal contents after the evisceration phase or from dirty surfaces [ 14 ]. (hindawi.com)
  • The objective of the present study was to perform a comprehensive molecular characterization of C. jejuni isolated from poultry on the farm and during the slaughter process. (hindawi.com)
  • PFGE has been successfully applied to track Campylobacter during poultry production [ 16 - 18 ] and, together with flaA -SVR sequencing, it represents a highly discriminatory method for a better understanding of Campylobacter population structures. (hindawi.com)
  • Campylobacter jejuni is a pathogen found in chickens and is the leading cause of foodborne bacterial diarrhoea in the USA, so poultry producers look for ways to control it before the birds go to processing. (thepoultrysite.com)
  • Raw and undercooked poultry and meat, raw milk and untreated water are sources for Campylobacter, the most common bacterial cause of diarrhea in the United States, according the U.S. Public Health Service. (thepoultrysite.com)
  • The failure to reduce the Campylobacter contamination of intensively reared poultry may be partially due to Campylobacter resisting disinfection in water after their internalization by waterborne protozoa. (asm.org)
  • Campylobacter jejuni and a variety of waterborne protozoa, including ciliates, flagellates, and alveolates, were detected in the drinking water of intensively reared poultry by a combination of culture and molecular techniques. (asm.org)
  • Humans are infected by ingestion of contaminated food, typically poultry, where C. jejuni is a frequent resident ( 6 , 11 ). (asm.org)
  • Campylobacter jejuni is an important human pathogen commonly associated with raw poultry. (barfblog.com)
  • The prevalence of C. jejuni in poultry products at retail stores was 58.97 per cent for flock 1 and 69.23 per cent for flock 2. (thepoultrysite.com)
  • The assay enables users to reliably detect and differentiate Campylobacter from poultry samples, without using gas jars, within 24 hours. (thermofisher.com)
  • Campylobacter is one of the leading causes of human foodborne illness in the United States, and epidemiological evidence indicates that poultry and poultry products are a significant source of human Campylobacter infections. (biomedsearch.com)
  • Reducing Campylobacter in the intestinal tract would reduce contamination of poultry products and eggs. (biomedsearch.com)
  • The sensitive nature of C. jejuni stands in contrast to the fact that it is difficult to eradicate from poultry production, and even more contradictory is the fact that the bacterium is able to survive the acidic passage through the human stomach. (diva-portal.org)
  • These findings may be important for our understanding of C. jejuni passage through the gastrointestinal tract and for hygiene practices used in poultry settings. (diva-portal.org)
  • Campylobacter jejuni is a pathogenic bacterium that causes gastroenteritis in humans yet is a widespread commensal in wild and domestic animals, particularly poultry. (asm.org)
  • The most common reservoir of C. jejuni transmission to humans is poultry, particularly chickens. (asm.org)
  • As C. jejuni infections commonly arise from contaminated poultry, phage treatments have been proposed to reduce the C. jejuni load on farms to prevent human infections. (bionity.com)
  • Studies on the pathogenesis of C. jejuni show that for this organism to cause disease, the susceptibility of the host and the relative virulence of the infecting strain are both important. (wikipedia.org)
  • Campylobacter jejuni is a microaerophilic organism, meaning that it requires an environment that contains a reduced concentration of oxygen (~3-5% of oxygen and ~2-10% of carbon dioxide). (kenyon.edu)
  • Thus, early administration of erythromycin significantly reduced the duration of both diarrhea and fecal excretion of the organism in infants and children with acute dysentery associated with C. jejuni. (nih.gov)
  • Since this organism usually causes systemic disease (as opposed to localized gastrointestinal disease caused by C. jejuni), the preferred anti-infective approach is parenteral. (renalandurologynews.com)
  • The chemotactic behavior of C. jejuni toward L-fucose, a constituent of both bile and mucin, may be an important factor in the affinity of the organism for the gallbladder and intestinal tract. (asm.org)
  • Campylobacter jejuni is a major zoonotic pathogen transmitted to humans via the food chain and is prevalent in chickens, a natural reservoir for this pathogenic organism. (asm.org)
  • Campylobacteriosis attributable to C. jejuni is a condition of emerging significance which arises principally from deficiencies in hygiene inherent in the environment and in the food chain which extends from domestic animals to the consumer. (springer.com)
  • In contrast to that better livestock-adapted groups characterized by the ability to utilize L-fucose and the presence of all of the ve identied putative C. jejuni iron-uptake systems as well as cj1321-cj1326, cj1365c, cj1585c, and cstII and/or cstIII (sialylated lipoologosaccharide) is more prevalent in animal hosts and was secondary associated with less severe campylobacteriosis. (isaude.net)
  • Campylobacter jejuni is the leading cause of human bacterial gastroenteritis, campylobacteriosis, in the world ( 1 , 11 , 21 ). (asm.org)
  • Due to the importance of macrolide antibiotics in clinical therapy of human campylobacteriosis, development of macrolide resistance in Campylobacter has become a concern for public health. (asm.org)
  • Campylobacter upsaliensis infections in humans can cause Campylobacteriosis, a more significant gastroenteritis. (wikipedia.org)
  • If left unchecked, gastroenteritis caused by C.jejuni ( campylobacterosis ) can progress to the autoimmune disease Guilain-Barre syndrome (GBS). (le.ac.uk)
  • Campylobacter jejuni Proteobacteria filumean eta Campylobacter generoan sailkatzen den bakterio bat da, gizakiak infektatzen dituena gastroenteritis akutuak sortuz. (wikipedia.org)
  • Campylobacter jejunik sortzen duen infekzioa (gastroenteritis) ur edo elikagai kutsatuen bidez harrapatzen da [1] . (wikipedia.org)
  • The Gram-negative bacterium Campylobacter jejuni , a leading cause of acute gastroenteritis in humans, is the most frequent antecedent pathogen. (pnas.org)
  • Campylobacter jejuni is a zoonotic pathogen and is one of the leading causes of human gastroenteritis worldwide. (asm.org)
  • Campylobacter jejuni ( C. jejuni ) is the most common cause of foodborne gastroenteritis worldwide. (mdpi.com)
  • Campylobacter jejuni is the most prevalent pathogenic bacterium of zoonotic gastroenteritis [ 1 ]. (mdpi.com)
  • Campylobacter is the most common cause of bacterial gastroenteritis in Europe. (hindawi.com)
  • Campylobacter jejuni infections are now the leading cause of bacterial gastroenteritis reported in the United States (2) . (cdc.gov)
  • In 1996, 46% of laboratory-confirmed cases of bacterial gastroenteritis reported in the Centers for Disease Control and Prevention/U.S. Department of Agriculture/Food and Drug Administration Collaborating Sites Foodborne Disease Active Surveillance Network were caused by Campylobacter species. (cdc.gov)
  • Campylobacter jejuni/coli have been known to be major bacterial causes of human gastroenteritis worldwide for decades. (umd.edu)
  • C. jejuni is a major agent of food-borne gastroenteritis. (warwick.ac.uk)
  • Campylobacter jejuni is one of the leading causes of gastroenteritis and food poisoning in humans. (medical-wiki.com)
  • Campylobacter is among the most common worldwide causes of bacterial gastroenteritis. (pubmedcentralcanada.ca)
  • Following an unusually heavy rainfall in June 2009, a community-wide outbreak of Campylobacter gastroenteritis occurred in a small Danish town. (ruc.dk)
  • Campylobacter jejuni and C. coli are human intestinal pathogens that are the most frequent causes of bacterial foodborne gastroenteritis in humans in the UK. (biomedcentral.com)
  • In dogs, Campylobacter upsaliensis can cause a mild to moderate form bacterial gastroenteritis. (wikipedia.org)
  • Individuals with recent C. jejuni infections develop Guillain-Barré syndrome at a rate of 0.3 per 1000 infections, about 100 times more often than the general population. (wikipedia.org)
  • In Guatemalan children, seven percent of the C. jejuni infections result in acute dysentery 2 . (springer.com)
  • Torres O.R., López-Ruano J.V., Cruz J.R., Cano F., Bartlett A. (1996) Campylobacter Jejuni Repeated Infections in Children from Rural Guatemala. (springer.com)
  • Guillain-Barré syndrome, the most frequent cause of acute neuromuscular paralysis, occurs 1-2 wk after various infections, in particular, Campylobacter jejuni enteritis. (pnas.org)
  • Although probiotics are effective in preventing other enteric infections, beneficial microorganisms have not been extensively studied with C. jejuni. (ei-resource.org)
  • In addition, an increasing proportion of human infections caused by C. jejuni are resistant to antimicrobial therapy. (cdc.gov)
  • Awareness of the public health implications of Campylobacter infections has evolved over more than a century (1) . (cdc.gov)
  • Less frequently, C . jejuni infections produce bacteremia, septic arthritis, and other extraintestinal symptoms (4) . (cdc.gov)
  • Most of these genetic studies, however, have employed a single strain or reference strains, and to date no studies have examined genetic diversity in a population of Campylobacter isolated from symptomatic and symptom-free infections and its relation to adherence and invasion of epithelial cells. (asm.org)
  • Taken together, the results suggest that protozoa may act as protective hosts against harsh conditions and might be a potential risk factor for C. jejuni infections. (diva-portal.org)
  • C. jejuni releases several different toxins, mainly enterotoxin and cytotoxins, which vary from strain to strain and correlate with the severity of the enteritis. (wikipedia.org)
  • On June 29, 2011, the Wyoming Department of Health was notified of two laboratory-confirmed cases of Campylobacter jejuni enteritis among persons working at a local sheep ranch. (cdc.gov)
  • Campylobacter enteritis in the Gambia. (springer.com)
  • 1979a) Campylobacter enteritis: Clinical and epidemiological features. (springer.com)
  • 1979b) Campylobacter enteritis associated with unpasteurized milk. (springer.com)
  • Campylobacter enteritis: a "new" disease. (atcc.org)
  • Ganglioside GM1 is an autoantigen for IgG Abs in patients with axonal GBS subsequent to C. jejuni enteritis ( 2 , 5 ). (pnas.org)
  • These results indicate that fresh mushrooms may indeed be a source of C. jejuni and support previously reported epidemiological data (Seattle-King County Department of Public Health, Surveillance of the Flow of Salmonella and Campylobacter in a Community, 1984) which revealed an an elevated relative risk of developing campylobacter enteritis in individuals who consume mushrooms. (asm.org)
  • Campylobacter jejuni is the most common cause of diarrhea in children of developing countries ( 4 ) and the primary cause of food-borne enteritis in industrialized regions ( 21 ). (asm.org)
  • Campylobacter (C.) jejuni is de meest frequente veroorzaker van gastro-enteritis in Nederland. (openrepository.com)
  • Campylobacter (C.) jejuni is identified as the major cause of bacterial gastro-enteritis in the Netherlands. (openrepository.com)
  • The prevalence of Campylobacter in broiler chicken flocks ranges from 3 to 90% depending on their location [ 6 , 7 ] and the isolation rates within positive flocks at slaughter are high (around 80%) [ 8 - 10 ]. (hindawi.com)
  • Recent studies have reported that the prevalence of Campylobacter in retail chicken products ranges from 90 to 100% across several countries [ 11 , 12 ]. (hindawi.com)
  • The resistance prevalence among C. jejuni from humans was 64.1 and 63.0% in Slovenia and Austria in 2013, respectively ( EFSA, 2015 ). (frontiersin.org)
  • Prevalence of Campylobacter jejuni, Campylobacter lari, and Campylobacter coli in different ecological guilds and taxa of migrating birds. (canarydatabase.org)
  • These findings clearly indicate that acquisition of macrolide resistance impairs the fitness and transmission of Campylobacter in chickens, suggesting that the prevalence of macrolide-resistant C. jejuni will likely decrease in the absence of antibiotic selection pressure. (asm.org)
  • High prevalence of thermophilic Campylobacter spp. (ajol.info)
  • Peak prevalence of C. jejuni in both cattle and sheep was observed during the summer and in cattle this apparent seasonality was associated with grazing pasture [odds ratio (OR) 2.14], while in sheep it was independent of grazing. (lancs.ac.uk)
  • The association between C.jejuni prevalence and diet in dairy cattle deserves further investigation. (lancs.ac.uk)
  • The review then focuses on the worldwide problem of C. jejuni antimicrobial resistance and mechanisms of pathogenesis and virulence. (nih.gov)
  • Taken together, these findings indicate that the ability of selected probiotics to prevent C. jejuni-mediated disease pathogenesis depends on the pathogen strain, probiotic strain and the epithelial cell type selected. (ei-resource.org)
  • Among the many causes of diarrhoea, C. jejuni 1 is seldom reported in the routine search for pathogens due to the special conditions it requires for isolation and growth. (springer.com)
  • Human colon T84 and embryonic intestine 407 epithelial cells were pretreated with Lactobacillus strains and then infected with two prototypic C. jejuni pathogens. (ei-resource.org)
  • C. jejuni strains isolated from such patients have a lipooligosaccharide (LOS) with a GM1-like structure ( 2 , 6 ). (pnas.org)
  • This survey looked at the proportion of C. jejuni and C. coli strains isolated from year 4 of the FSA's UK retail chicken survey that were resistant to a range of antimicrobial agents. (food.gov.uk)
  • The 66 C. jejuni strains isolated were submitted to genetic characterization. (scielo.br)
  • The DLST scheme was validated using 50 clinical and environmental C. jejuni strains isolated from human (C5, H, H15-H19), chicken (CH1-CH15), water (W2-W17), and ovine samples (OV1-OV6). (edu.au)
  • In this study, we aimed to characterise the metabolic diversity of both C. jejuni and C. coli using a diverse panel of clinical strains isolated from the UK, Pakistan and Thailand, thereby representing both the developed and developing world. (biomedcentral.com)
  • Campylobacter jejuni is the most common bacterial cause of enterocolitis in humans, leading to diarrhoea and chronic extraintestinal diseases. (ei-resource.org)
  • Diversities and similarities in PFGE profiles of Campylobacter jejuni isolated from migrating birds and humans. (canarydatabase.org)
  • Phenotypic and genotypic relationship between Campylobacter spp isolated from humans and chickens in Northern Ireland--a comparison of three phenotyping and two genotyping schemes. (canarydatabase.org)
  • Campylobacter jejuni is the leading bacterial cause of gastrointestinal diarrheal disease in humans worldwide. (diva-portal.org)
  • In this work, we compared the phenotypic and transcriptional responses of C. jejuni to intestinal compositions of humans (disease-susceptible host) and chickens (zoonotic host) by using human fecal and chicken cecal extracts. (asm.org)
  • Campylobacter jejuni is a frequent foodborne pathogen of humans. (bionity.com)
  • C. upsaliensis is the second most common Campylobacter species isolated in humans with diarrhea (behind Campylobacter jejuni). (wikipedia.org)
  • However, during the past decades Campylobacter has become increasingly resistant to clinically important antimicrobial agents, compromising the effectiveness of clinical therapy ( 17 ). (asm.org)
  • jejuni NCTC 11168 complete genome. (atcc.org)
  • and H. hepaticus ATCC 51449 ( 51 )-and five from the Campylobacteraceae-C. jejuni NCTC 11168 and RM1221, Campylobacter lari RM2100, Campylobacter upsaliensis RM3195, and Campylobacter coli RM2228 ( 22 , 46 ). (asm.org)
  • N-terminal sequencing allowed us to identify the predicted coding sequence Cj1170c from the Campylobacter jejuni genome database as the corresponding gene in the NCTC 11168 genome sequence. (biochemj.org)
  • [2] Campylobacter jejuni is the number one cause of food-borne illness in the United States. (kenyon.edu)
  • Although it is the leading cause of food-borne illness in the United States, Campylobacter can be easily killed by cooking or heating. (kenyon.edu)
  • Campylobacter jejuni is one of the most common causes of bacterial diarrhea worldwide and is the primary bacterial cause of food-borne illness. (asm.org)
  • Campylobacter jejuni is one of the main causes of bacterial food-borne illness worldwide. (asm.org)
  • Campylobacter species are a major cause of food-borne disease in the developed and the developing world. (biomedcentral.com)
  • CheY-mediated modulation of Campylobacter jejuni virulence. (nih.gov)
  • In this study, we constructed an insertion mutant and complement of this gene in C. jejuni and examined changes in virulence. (frontiersin.org)
  • We present data that collectively significantly proves our hypothesis that cj0371 is a new virulence-associated gene and through the influence of chemotaxis plays a negative role in C. jejuni pathogenicity. (frontiersin.org)
  • Despite the availability of both genomic information of different C. jejuni strains and genetic tools, the complete understanding of the virulence of C. jejuni is still an ongoing effort. (frontiersin.org)
  • Multiple virulence factors have roles in Campylobacter-intestinal epithelial cell interaction. (umd.edu)
  • it should be demonstrated to which of the previous defined groups these Campylobacter-subpopulations, associated with higher virulence, correspond.Results:Besides the marker gene pldA, all new estimated genetic markers show significant differences in their distribution among the various MLST-based groups. (isaude.net)
  • Research in the virulence characteristics of this pathogen is currently very limited due to its high susceptibility to the antibiotics commonly used in Campylobacter selective media. (wikipedia.org)
  • Campylobacter jejuni strain 81-176 uptake into 407 intestinal cells and Citrobacter entry into T24 bladder cells was blocked by microtubule depolymerization and inhibitors of coated-pit formation but not by microfilament depolymerization. (pnas.org)
  • We have studied the uptake and intracellular trafficking of viable and heat killed bacterial cells of the C. jejuni strain 81-176 in A. polyphaga . (diva-portal.org)
  • The indirect C. jejuni -induced barrier defects and its protection by curcumin were analyzed in co-cultures with HT-29/B6-GR/MR epithelial cells together with differentiated THP-1 immune cells. (mdpi.com)
  • Strain-specific probiotic (Lactobacillus helveticus) inhibition of Campylobacter jejuni invasion of human intestinal epithelial cells. (ei-resource.org)
  • C. jejuni /coli-induced proinflammatory cytokine Interleukin (IL)-8 secretion in polarized human colonic epithelial cells T84 was examined, and the role of NF-κB pathway in Campylobacter-induced IL-8 secretion was determined. (umd.edu)
  • Data suggested that C. jejuni/coli induce basolateral-polarized secretion of IL-8 in human intestinal epithelial cells, and C. jejuni-induced IL-8 secretion is NF-κB-dependent. (umd.edu)
  • Adherence to and invasion of epithelial cells are the most important pathogenic mechanisms of Campylobacter diarrhea. (asm.org)
  • Several studies have focused on the characterization of C. jejuni adhesins and binding factors that enable some strains to adhere to and invade epithelial cells ( 10 , 13 , 31 ). (asm.org)
  • It invades the epithelial cells of the jejunum, ileum and the colon. (medical-wiki.com)
  • In 1886 a pediatrician, Theodor Escherich, observed Campylobacters from diarrhea samples of children. (wikipedia.org)
  • The first isolation of C. jejuni was in Brussels, Belgium, from stool samples of a patient with diarrhea. (wikipedia.org)
  • Campylobacter jejuni is one of the most common bacterial causes of diarrhea. (medlineplus.gov)
  • Campylobacter jejuni is present in high levels in diarrhea stools of infected individuals as well as animal feces. (kenyon.edu)
  • To evaluate the efficacy of early treatment with erythromycin on the duration of fecal excretion and of diarrhea associated with Campylobacter jejuni, 170 patients, age 3 to 60 months, were randomly assigned in a double-blind fashion to receive either erythromycin ethyl succinate or placebo immediately after being seen at Cayetano Heredia Hospital because of acute dysentery. (nih.gov)
  • Campylobacter jejuni is the major cause of human bacterial diarrhea worldwide. (frontiersin.org)
  • In 1886, Escherich observed organisms resembling campylobacters in stool samples of children with diarrhea. (cdc.gov)
  • In 1957, King described the isolation of related Vibrio from blood samples of children with diarrhea, and in 1972, clinical microbiologists in Belgium first isolated campylobacters from stool samples of patients with diarrhea (1) . (cdc.gov)
  • In one study, approximately half of the patients with laboratory-confirmed campylobacter- iosis reported a history of bloody diarrhea (3) . (cdc.gov)
  • There is a good correlation between the clinical presentation of diarrhea and the isolation of Campylobacter strains that adhere to and invade HEp-2 cells. (asm.org)
  • Epidemiological association of Campylobacter jejuni groups with. (isaude.net)
  • Rapid detection and identification of C. jejuni informs timely prescription of appropriate therapeutics and epidemiological investigations. (rsc.org)
  • The biotyping scheme descriptionbed provides separation of strains within Campylobacter jejuni/coli which is of value for epidemiological studies. (journals.co.za)
  • Serotyping using the polyvalent campylobacter antisera is also of value, but a combination of biotyping and serotyping is likely to provide more specific epidemiological information. (journals.co.za)
  • The DLST showed discriminatory power above 0.95 and excellent congruence to multilocus sequence typing and can be recommended as a rapid and low-cost typing scheme for epidemiological investigation of C. jejuni. (edu.au)
  • Dr Hanning looked at the ability of C. jejuni to survive from biofilm populations isolated from four places: a drinking unit in a chicken grow-out house, a drain under a plucker in a processing plant, a retail chicken carcass and a crate used to haul live chickens. (thepoultrysite.com)
  • Caprylic acid supplemented in feed reduces enteric Campylobacter jejuni colonization in ten-day-old broiler chickens. (biomedsearch.com)
  • It has, however, not been tested in the control of Campylobacter in chickens. (biomedsearch.com)
  • These data suggest that low-dose supplementation with caprylic acid in feed may reduce Campylobacter colonization in young chickens. (biomedsearch.com)
  • In this study we conducted pairwise competitions and comingling experiments in chickens using clonally related and isogenic C. jejuni strains, which are either susceptible or resistant to erythromycin (Ery). (asm.org)
  • There are recent evidence indicating that the continuous use of a macrolide at subtherapeutic level in chickens results in the development of Ery resistance in Campylobacter ( 32 , 34 ). (asm.org)
  • 1982). Campylobacter jejuni survival in chicken meat as a function of temperature. (springer.com)
  • Immunopathology and Th1/Th2 immune response of Campylobacter jejuni -induced paralysis resembling Guillain-Barré syndrome in chicken," Medical Microbiology and Immunology , vol. 201, no. 2, pp. 177-187, 2012. (hindawi.com)
  • The overall chicken survey tested 1,769 samples of whole, UK-produced, fresh chicken during the period August 2017 to July 2018 for Campylobacter. (food.gov.uk)
  • Using RNA sequencing, we assessed C. jejuni transcriptional responses to medium supplemented with human fecal versus chicken cecal extracts and in extract-supplemented medium versus medium alone. (asm.org)
  • C. jejuni can colonize chicken ceca, which are two blind pouches at the junction of the small and large intestines, to concentrations as high as 2.5 × 10 9 CFU/g cecal material without signs of pathology ( 7 , 8 ). (asm.org)
  • Serine, aspartic acid, glutamic acid and proline are the most common amino acids found in chicken faeces [ 4 ],[ 12 ] and are therefore possible nutrient sources for C. jejuni in the chicken gut. (biomedcentral.com)
  • The clinical importance of emerging Campylobacter species. (cdc.gov)
  • Therefore, elucidating the mechanisms involved in DNA transfer will help us understand the adaptation of H. pylori and C. jejuni to changing environmental conditions and may have clinical relevance in the area of antibiotic resistance. (asm.org)
  • Results GF Apc Min/+ mice colonised with human clinical isolate C. jejuni 81-176 developed significantly more and larger tumours when compared with uninfected mice. (bmj.com)
  • Conclusion Human clinical isolate C. jejuni 81-176 promotes colorectal cancer and induces changes in microbial composition and transcriptomic responses, a process dependent on CDT production. (bmj.com)
  • The serology indicated that asymptomatic persons from the same household may have been exposed, during the outbreak period, to Campylobacter at doses that did not elicit symptoms or alternatively had been exposed to Campylobacter at a time prior to the outbreak, resulting in residual immunity and thus absence of clinical signs. (ruc.dk)
  • Results shown significant reductions in adherence (up to 70%) of all C. jejuni strains and for C. coli ATCC 43485 in presence of MOS or pMOS (50 mg/mL). (unl.edu)
  • The DksA-like protein of C. jejuni (CJJ81176_0160, YP_999849.1) was aligned with DksA homologs of Campylobacter coli RM2228 (ZP_00367836.1), H. hepaticus ATCC 51449 (NP_860767.1), W. succinogenes DSM 1740 (NP_906418.1) E. coli K-12 (NP_414687.1), and P. aeruginosa PAO1 (NP_253411.1). (asm.org)
  • An in vitro assay showed that C. jejuni remained viable when internalized by Tetrahymena pyriformis and Acanthamoeba castellanii for significantly longer (up to 36 h) than when they were in purely a planktonic state. (asm.org)
  • The addition of either purified S100A12 or of calprotectin to in vitro cultures of C. jejuni was found to inhibit growth in a zinc-dependent manner. (tennessee.edu)
  • Macrolide-resistant mutants of Campylobacter jejuni and Campylobacter coli were selected in vitro using erythromycin and tylosin. (asm.org)
  • Coloured scanning electron micrograph (SEM) of Campylobacter jejuni is an enteric, curved-rod prokaryote (bacterium). (sciencephoto.com)
  • Campylobacter jejuni is a gut colonizer of numerous animal species and a major human enteric pathogen. (datadryad.org)
  • This is what campylobacter organisms look like through a microscope. (medlineplus.gov)
  • In: proceedings 10th international workshop on Campylobacter, Helicobacter and related organisms : Baltimore, USA, 1999 - p. 25 - 25. (wur.nl)
  • The internalized Campylobacter were also significantly more resistant to disinfection than planktonic organisms. (asm.org)
  • The zoonotic microorganism Campylobacter is a leading cause of human diarrheal disease ( 9 ). (asm.org)
  • The experiments were conducted with Campylobacter jejuni, Salmonella, and Escherichia coli. (wur.nl)
  • Recipient(s) will receive an email with a link to 'Sunlight inactivation of Campylobacter jejuni and Salmonella enterica, compared with Escherichia coli, in seawater and river water' and will not need an account to access the content. (iwaponline.com)
  • The inactivation of Campylobacter jejuni and Salmonella enterica , compared with Escherichia coli , was determined in 100 l chambers of seawater and river water located at an outdoor site. (iwaponline.com)
  • Campylobacter is a helical-shaped, non-spore-forming, Gram-negative, microaerophilic, nonfermenting motile bacterium with a single flagellum at one or both poles, which are also oxidase-positive and grow optimally at 37 to 42 °C. When exposed to atmospheric oxygen, C. jejuni is able to change into a coccal form. (wikipedia.org)
  • Campylobacter jejuni is a Gram-negative, spiral-shaped bacterium. (le.ac.uk)
  • Campylobacter jejuni, from the delta-epsilon group of proteobacteria, is a microaerophilic, Gram-negative, flagellate, spiral bacterium-properties it shares with the related gastric pathogen Helicobacter pylori. (nih.gov)
  • The Gram-negative bacterium Campylobacter jejuni is able to enter, survive and multiply within the free living amoeba Acanthamoeba polyphaga , but the molecular mechanisms behind these events are still unclear. (diva-portal.org)
  • Campylobacter jejuni is a bacterium found primarily in the intestinal gut of animals and birds and shed primarily through the feces. (barfblog.com)
  • Studies that investigate arsenic resistance in the foodborne bacterium Campylobacter are limited. (mdpi.com)
  • However, much less is known about the epidemiology of C. jejuni in the environment and what mechanisms the bacterium depends on to tolerate low pH. (diva-portal.org)
  • We also found that A. polyphaga could protect C. jejuni in acid environments with pH levels far below the range in which the bacterium normally survives. (diva-portal.org)
  • Here, for the first time, we report the applicability of Raman spectroscopy, surface-enhanced Raman scattering (SERS) and matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-TOF-MS) combined with chemometrics, for rapid differentiation and characterisation of mutants of a single isogenic C. jejuni strain that disrupt the production of prominent surface features (capsule, flagella and glycoproteins) of the bacterium. (rsc.org)
  • Some past studies have begun to define the metabolic requirements of C. jejuni , [ 4 ]-[ 7 ], yet there is still a paucity of information regarding the metabolic flexibility of this bacterium, which is able to survive in such a wide range of environmental niches. (biomedcentral.com)
  • Molecular epidemiology of Campylobacter jejuni in broiler flocks using randomly amplified polymorphic DNA-PCR and 23S rRNA-PCR and role of litter in its transmission. (canarydatabase.org)
  • Collectively, our results strongly suggest that protozoa in broiler drinking water systems can delay the decline of Campylobacter viability and increase Campylobacter disinfection resistance, thus increasing the potential of Campylobacter to colonize broilers. (asm.org)
  • The results suggest that, in sunlight-exposed waters, E. coli will be a more conservative indicator for C. jejuni than for S. enterica , and C. jejuni transmission as a pathogenic agent is less likely than for S. enterica . (iwaponline.com)
  • On sensitization with C. jejuni lipooligosaccharide, rabbits developed anti-GM1 IgG antibody and flaccid limb weakness. (pnas.org)
  • The following product was used in this experiment: Campylobacter jejuni Monoclonal Antibody (H46G) from Thermo Fisher Scientific, catalog # MA1-7472, RRID AB_1016703. (thermofisher.com)
  • The MA1-7472 antibody reacts with Campylobacter jejuni 100 kDa in bacterial and infected tissue samples. (thermofisher.com)
  • The MA1-7472 antibody was raised against the C. jejuni 100 kDa antigen. (thermofisher.com)
  • This antibody recognizes Campylobacter jejuni. (genetex.com)
  • European Robins infected with the wild bird isolate generated a specific antibody response to C. jejuni membrane proteins from the avian isolate, which also was cross-reactive to membrane proteins of the human isolate. (lu.se)
  • Serum antibody levels against Campylobacter were significantly higher in cases than in asymptomatic persons. (ruc.dk)
  • Campylobacter jejuni is a leading bacterial cause of human foodborne illness worldwide in both developed and developing nations ( 1 , 2 ). (asm.org)
  • Here we report the genome sequence of C. jejuni NCTC11168. (nih.gov)
  • The genome sequence of C. jejuni codes for respiratory components that can utilize multiple electron donors and acceptors in a branched electron transport chain ( 27 ). (asm.org)
  • The fact that complex I occurs in the C. jejuni genome sequence was somewhat surprising, as it has been shown elsewhere that NADH is a poor respiratory electron donor ( 14 ). (asm.org)
  • Since the publication of the C. jejuni NCTC11168 genome sequence in 2000, there has been a spectacular increase in research on this important human pathogen. (biomedcentral.com)
  • Research over the last 7 years on C. jejuni , coupled with the publication of a further 2 C. jejuni genome sequences [ 15 , 16 ] and another 3 Campylobacter species [ 15 ], has heightened the need for re-analysis of the original NCTC11168 genome sequence. (biomedcentral.com)
  • Inhibitors of endosome acidification had no significant impact on intracellular survival of Campylobacter jejuni or Citrobacter freundii, but monensin markedly reduced Citrobacter uptake. (pnas.org)
  • 1983). Isolation of Campylobacter jejuni from an aborted caprine fetus. (springer.com)
  • Billingham, J.D. (1981a) A comparison of two media for the isolation of campylobacter in the tropics. (springer.com)
  • Isolation of Campylobacter fetus ssp. (springer.com)
  • Isolation of Campylobacter jejuni from retail mushrooms. (asm.org)
  • The results indicate that the presented method in this study with sensitivity equal to the PCR is useful for isolation of Campylobacter spp. (magiran.com)
  • Faecal samples were collected and cultured using standard techniques for isolation of Campylobacter. (lancs.ac.uk)
  • Campylobacter jejuni contains multiple hypermutable sequences. (warwick.ac.uk)
  • A combination of experimental and theoretical modelling will be utilised to determine how the mutation rates of these hypermutable sequences enable Campylobacters to survive the combined effects of fluctuating selection and non-selective bottlenecks. (warwick.ac.uk)
  • The hypermutable sequences of C. jejuni are mainly polyG tracts whose mutations mediate ON/OFF switches in gene expression. (warwick.ac.uk)
  • Sequences of every alleles for MLST profiles of 3,834 STs present in the pubMLST Campylobacter database as of the 26th of May 2011 and used in the associated publication. (datadryad.org)
  • The C. jejuni NCTC11168 re-annotation reduced the total number of coding sequences from 1654 to 1643, of which 90.0% have additional information regarding the identification of new motifs and/or relevant literature. (biomedcentral.com)
  • Campylobacter jejuni is a common raw milk contaminant and is notoriously difficult to isolate from food products, because of its fastidious growth requirements. (cdc.gov)
  • These were selected as every fourth positive Campylobacter isolate (or next viable isolate) but selection was adjusted to ensure adequate representation of producer premises and retailers, as deduced from market share data. (food.gov.uk)
  • In addition to chemotaxis, motility in C.jejuni is also controlled by a process called phase variation . (le.ac.uk)
  • In May 2014, the Utah Public Health Laboratory (UPHL) notified the Utah Department of Health (UDOH) of specimens from three patients infected with Campylobacter jejuni yielding indistinguishable pulsed-field gel electrophoresis (PFGE) patterns. (cdc.gov)
  • All suspected Campylobacter colonies were identified with PCR assays and C. jejuni was genotyped by sequence analysis of the flaA short variable region (SVR) and by pulsed-field gel electrophoresis (PFGE) using SmaI enzyme. (hindawi.com)
  • SmaI divided 122 C. jejuni strains from flock 1 and 106 from flock 2 into 17 and 13 PFGE types, respectively. (thepoultrysite.com)
  • Four motile, non-adherent and non-invasive mutants of Campylobacter jejuni 81-176 generated by a site-specific insertional mutagenesis scheme were characterized at the molecular level and all contained a duplication of the same region of the chromosome. (nih.gov)
  • To verify that molecular mimicry between an environmental agent and the peripheral nerves causes GBS, we sensitized animals with C. jejuni LOS and produced a replica of human GBS, generated anti-GM1 mAb by immunization with the LOS, and determined the distribution of GM1 in human spinal nerve roots. (pnas.org)
  • Caracterização molecular de linhagens de Campylobacter jejuni de origens diversas. (usp.br)
  • The goal of this research was to assess the anti-adherence activity of mannan oligosaccharides (MOS), pectic oligosaccharides (POS) and cranberry high molecular weight component (HMW) against Campylobacter jejuni and Campylobacter coli . (unl.edu)
  • This is the first study of molecular subtyping analysis of human and animal C. jejuni strains using sequencing technique and genealogic analysis in the state of São Paulo, Brazil. (scielo.br)
  • Este foi o primeiro estudo de subtipagem molecular de estirpes de C. jejuni de origem humana e animal utilizando a técnica do seqüenciamento com análise genealógica realizado no Estado de São Paulo, Brasil. (scielo.br)
  • The molecular mechanism behind this phenomenon involves nuclear translocation by Cas9, a nuclease in C. jejuni (CjeCas9) that is the molecular marker of the Type II CRISPR-Cas system. (eur.nl)
  • A novel pore-forming protein identified in Campylobacter was purified by ion-exchange chromatography and named Omp50 according to both its molecular mass and its outer membrane localization. (biochemj.org)
  • Rapidly detect Campylobacter jejuni, C. coli and C. lari in food and environmental samples with the Thermo Scientific™ SureTect™ Campylobacter jejuni, C. coli and C. lari PCR Assay. (thermofisher.com)
  • The lysate is then loaded into the SureTect Campylobacter jejuni, C. coli and C. lari PCR tube to re-hydrate the pre-loaded PCR pellet which contains all the necessary components and reagents for PCR, including a probe, primers and DNA template for the internal amplification control (IAC). (thermofisher.com)
  • Omp50 is expressed in C. jejuni and Campylobacter lari but not in Campylobacter coli . (biochemj.org)
  • Recently, we demonstrated the existence of six C. jejuni-groups by combining MLST with six genetic markers. (isaude.net)
  • C. jejuni is also commonly found in animal feces . (eol.org)
  • In the European Union, Campylobacter is still the most commonly reported cause of bacterial foodborne illness with a notification rate of 55.49 cases per 100,000 of population in 2012 [ 2 ]. (hindawi.com)
  • Moreover, the protein showed only weak sequence identity with the major outer-membrane protein (MOMP) of Campylobacter , correlating with the absence of antigenic cross-reactivity between these two proteins. (biochemj.org)
  • To improve our understanding of this important human pathogen, the C. jejuni NCTC11168 genome was sequenced and published in 2000. (biomedcentral.com)
  • C. jejuni is not normally distributed among Antarctic animals ( 1 , 2 ), which indicates that this strain may have been imported through human activities. (cdc.gov)
  • Occurrence of Campylobacter jejuni in pets living with human patients infected with C. jejuni. (canarydatabase.org)
  • In the United States, an estimated 2.1 to 2.4 million cases of human campylobacter- iosis (illnesses ranging from loose stools to dysentery) occur each year (2) . (cdc.gov)
  • To investigate shedding patterns and putative effects on an avian host, we developed a colonization model in which a wild bird species, the European Robin Erithacus rubecula, was inoculated orally with C. jejuni from either a human patient or from another wild bird species, the Song Thrush Turdus philomelos. (lu.se)
  • One subtype was identical to a C. jejuni strain of human origin with the sequence in the GenBank (GENBANK accession number AF050186). (scielo.br)
  • Collectively, this implicates a function for the 1649-1656 gene cluster in C. jejuni iron scavenging and stress survival in the human intestinal environment. (asm.org)
  • Campylobacter jejuni has been recognized as one of the most common causes of human enterocolitis worldwide ( 2 ). (asm.org)
  • Over 80% of human cases are caused by Campylobacter jejuni and around 10% by Campylobacter coli, with the remaining human cases caused by other Campylobacter species. (biomedcentral.com)
  • Longitudinal study of the excretion patterns of thermophilic Campylobacter spp. (canarydatabase.org)
  • A total of 200 freshly voided goat faeces samples were collected in the study and were examined for the presence of thermophilic Campylobacter spp. (ajol.info)
  • The sequence of Campylobacter jejuni is variable. (kenyon.edu)
  • Subtyping analysis of C. jejuni strains based on sequencing of the fla A gene variable region and analysis of sequence alignment by the Maximum Parsimony method showed to be highly discriminatory, providing the best conditions to differentiate strains involved in outbreaks from those sporadically isolated. (scielo.br)
  • The re-annotation also utilises sequence data from additional Campylobacter strains and species not available during the original annotation. (biomedcentral.com)
  • Campylobacter jejuni belonging to two related flaA types were isolated from stool samples. (ruc.dk)
  • Evidence for inter-species recombination of the flagellin (flaA) locus between Campylobacter coli and Campylobacter jejuni. (lancs.ac.uk)
  • We show that the Campylobacter jejuni mfrABE operon is transcribed from a single promoter, with the mfrA gene preceded by a small open reading-frame ( mfrX ) encoding a C. jejuni -specific polypeptide of unknown function. (wiley.com)
  • In this thesis, I have shown that C. jejuni actively invades Acanthamoeba polyphaga . (diva-portal.org)
  • The overall aim of this thesis was to explore the role of amoebae of the genus Acanthamoeba as an intermediate host and vector for survival and dissemination of C. jejuni . (diva-portal.org)
  • Although multiple mechanisms of macrolide resistance have been reported in different bacterial genus and species, modifications of the ribosomal target sites (e.g., the 23S rRNA gene and ribosomal proteins L4 and L22) and active efflux via the CmeABC efflux pump are the major mechanisms conferring macrolide resistance in Campylobacter ( 13 , 19 , 20 , 41 ). (asm.org)
  • As C. jejuni is only susceptible to erythromycin, it is crucial to make the differential diagnosis from dysentery caused by Shigellae or by Entamoeba histolytica , in order to prescribe the appropriate treatment 3 . (springer.com)
  • Comparable occurrences of resistance were observed among C. jejuni and C. col i to erythromycin and nalidixic acid. (ajol.info)
  • In addition, we localized the Cj0371 protein at the poles of C. jejuni by fluorescence microscopy. (frontiersin.org)
  • The electron acceptor for 2-oxoglutarate:acceptor oxidoreductase was determined to be flavodoxin, which was also determined to be an essential protein in C. jejuni . (asm.org)
  • Involvement of the DksA-like protein in iron-related metabolism of C. jejuni . (asm.org)
  • Specifically, our results reveal that CjeCas9 activates DNA damage (p53, ATM (Ataxia Telangiectasia Mutated Protein)), pro-inflammatory (NF-κB (Nuclear factor-κB)) signaling and cell death pathways, driving Caco-2 cells infected by wild-type C. jejuni, but not when infected by a cas9 deletion mutant, towards programmed cell death. (eur.nl)