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CampylobacterCampylobacter jejuniCampylobacter InfectionsCampylobacter fetusCampylobacter coliCampylobacter lariEnteritisChickensPoultry DiseasesFecesPoultryFood MicrobiologyCampylobacter rectusFlagellinGuillain-Barre SyndromeDNA, BacterialAbattoirsDiarrheaPoultry ProductsBacterial Typing TechniquesGastroenteritisArcobacterPolyradiculoneuropathyCampylobacter hyointestinalisBacterial ProteinsCampylobacter upsaliensisSerotypingCloacaAnimals, DomesticHippuratesDrug Resistance, BacterialFoodborne DiseasesMeatFood ContaminationCecumAnti-Bacterial AgentsColony Count, MicrobialCulture MediaSequence Analysis, DNAMicrobial Sensitivity TestsErythromycinPolymerase Chain ReactionFood-Processing IndustrySalmonellaElectrophoresis, Gel, Pulsed-FieldBacteriological TechniquesSpecies SpecificityMolecular Sequence DataGenes, BacterialFood HandlingCiprofloxacinCampylobacter sputorumAnimal HusbandryFlagellaMultilocus Sequence TypingMiller Fisher SyndromeTurkeysBacterial SheddingCattlePolymorphism, Restriction Fragment LengthDisease ReservoirsRNA, Ribosomal, 16SDNA FingerprintingWater MicrobiologyHelicobacterAbortion, SepticRNA, Ribosomal, 23SSeasonsAntibodies, BacterialZoonosesBacteroidaceaeCattle DiseasesBacterial AdhesionNalidixic AcidFluoroquinolonesAntigens, BacterialDisease OutbreaksVirulenceGastritisMilkDrug Resistance, MicrobialGenome, BacterialAnti-Infective AgentsBase SequenceIntestinesDNA, RibosomalBirdsGenotypeNucleic Acid HybridizationMolecular MimicryMicrobial ViabilityMeat ProductsEnvironmental MicrobiologyTravelPhylogenyShigellaMolecular EpidemiologyGene Expression Regulation, Bacterial
Campylobacter
A genus of bacteria found in the reproductive organs, intestinal tract, and oral cavity of animals and man. Some species are pathogenic.
Campylobacter jejuni
A species of bacteria that resemble small tightly coiled spirals. Its organisms are known to cause abortion in sheep and fever and enteritis in man and may be associated with enteric diseases of calves, lambs, and other animals.
Campylobacter Infections
Infections with bacteria of the genus CAMPYLOBACTER.
Campylobacter fetus
A species of bacteria present in man and many kinds of animals and birds, often causing infertility and/or abortion.
Campylobacter coli
A species of gram-negative, rod-shaped bacteria isolated from the intestinal tract of swine, poultry, and man. It may be pathogenic.
Campylobacter lari
A species of thermophilic CAMPYLOBACTER found in healthy seagulls and causing ENTERITIS in humans.
Enteritis
Inflammation of any segment of the SMALL INTESTINE.
Chickens
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
Poultry Diseases
Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild.
Feces
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
Poultry
Domesticated birds raised for food. It typically includes CHICKENS; TURKEYS, DUCKS; GEESE; and others.
Food Microbiology
The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.
Campylobacter rectus
A species of CAMPYLOBACTER isolated from cases of human PERIODONTITIS. It is a microaerophile, capable of respiring with OXYGEN.
Flagellin
A protein with a molecular weight of 40,000 isolated from bacterial flagella. At appropriate pH and salt concentration, three flagellin monomers can spontaneously reaggregate to form structures which appear identical to intact flagella.
Guillain-Barre Syndrome
An acute inflammatory autoimmune neuritis caused by T cell- mediated cellular immune response directed towards peripheral myelin. Demyelination occurs in peripheral nerves and nerve roots. The process is often preceded by a viral or bacterial infection, surgery, immunization, lymphoma, or exposure to toxins. Common clinical manifestations include progressive weakness, loss of sensation, and loss of deep tendon reflexes. Weakness of respiratory muscles and autonomic dysfunction may occur. (From Adams et al., Principles of Neurology, 6th ed, pp1312-1314)
DNA, Bacterial
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Abattoirs
Places where animals are slaughtered and dressed for market.
Diarrhea
An increased liquidity or decreased consistency of FECES, such as running stool. Fecal consistency is related to the ratio of water-holding capacity of insoluble solids to total water, rather than the amount of water present. Diarrhea is not hyperdefecation or increased fecal weight.
Poultry Products
Food products manufactured from poultry.
Bacterial Typing Techniques
Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.
Gastroenteritis
INFLAMMATION of any segment of the GASTROINTESTINAL TRACT from ESOPHAGUS to RECTUM. Causes of gastroenteritis are many including genetic, infection, HYPERSENSITIVITY, drug effects, and CANCER.
Arcobacter
A genus of gram-negative, aerotolerant, spiral-shaped bacteria isolated from water and associated with diarrhea in humans and animals.
Polyradiculoneuropathy
Diseases characterized by injury or dysfunction involving multiple peripheral nerves and nerve roots. The process may primarily affect myelin or nerve axons. Two of the more common demyelinating forms are acute inflammatory polyradiculopathy (GUILLAIN-BARRE SYNDROME) and POLYRADICULONEUROPATHY, CHRONIC INFLAMMATORY DEMYELINATING. Polyradiculoneuritis refers to inflammation of multiple peripheral nerves and spinal nerve roots.
Campylobacter hyointestinalis
A species of CAMPYLOBACTER isolated from the INTESTINES of PIGS with proliferative ENTERITIS. It is also found in CATTLE and in CRICETINAE and can cause enteritis in humans.
Bacterial Proteins
Proteins found in any species of bacterium.
Campylobacter upsaliensis
A species of CAMPYLOBACTER isolated from DOGS; CATS; and humans.
Serotyping
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
Cloaca
A dilated cavity extended caudally from the hindgut. In adult birds, reptiles, amphibians, and many fishes but few mammals, cloaca is a common chamber into which the digestive, urinary and reproductive tracts discharge their contents. In most mammals, cloaca gives rise to LARGE INTESTINE; URINARY BLADDER; and GENITALIA.
Animals, Domestic
Animals which have become adapted through breeding in captivity to a life intimately associated with humans. They include animals domesticated by humans to live and breed in a tame condition on farms or ranches for economic reasons, including LIVESTOCK (specifically CATTLE; SHEEP; HORSES; etc.), POULTRY; and those raised or kept for pleasure and companionship, e.g., PETS; or specifically DOGS; CATS; etc.
Hippurates
Salts and esters of hippuric acid.
Drug Resistance, Bacterial
The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
Foodborne Diseases
Acute illnesses, usually affecting the GASTROINTESTINAL TRACT, brought on by consuming contaminated food or beverages. Most of these diseases are infectious, caused by a variety of bacteria, viruses, or parasites that can be foodborne. Sometimes the diseases are caused by harmful toxins from the microbes or other chemicals present in the food. Especially in the latter case, the condition is often called food poisoning.
Meat
The edible portions of any animal used for food including domestic mammals (the major ones being cattle, swine, and sheep) along with poultry, fish, shellfish, and game.
Food Contamination
The presence in food of harmful, unpalatable, or otherwise objectionable foreign substances, e.g. chemicals, microorganisms or diluents, before, during, or after processing or storage.
Cecum
The blind sac or outpouching area of the LARGE INTESTINE that is below the entrance of the SMALL INTESTINE. It has a worm-like extension, the vermiform APPENDIX.
Anti-Bacterial Agents
Substances that reduce the growth or reproduction of BACTERIA.
Colony Count, Microbial
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Sequence Analysis, DNA
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Microbial Sensitivity Tests
Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).
Erythromycin
A bacteriostatic antibiotic macrolide produced by Streptomyces erythreus. Erythromycin A is considered its major active component. In sensitive organisms, it inhibits protein synthesis by binding to 50S ribosomal subunits. This binding process inhibits peptidyl transferase activity and interferes with translocation of amino acids during translation and assembly of proteins.
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Food-Processing Industry
The productive enterprises concerned with food processing.
Salmonella
A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.
Electrophoresis, Gel, Pulsed-Field
Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.
Bacteriological Techniques
Techniques used in studying bacteria.
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Genes, Bacterial
The functional hereditary units of BACTERIA.
Food Handling
Any aspect of the operations in the preparation, processing, transport, storage, packaging, wrapping, exposure for sale, service, or delivery of food.
Ciprofloxacin
A broad-spectrum antimicrobial carboxyfluoroquinoline.
Campylobacter sputorum
A species of CAMPYLOBACTER comprised of three biovars based on their reaction to CATALASE and UREASE. They have been isolated from humans, CATTLE, and SHEEP.
Animal Husbandry
The science of breeding, feeding and care of domestic animals; includes housing and nutrition.
Flagella
A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
Multilocus Sequence Typing
Direct nucleotide sequencing of gene fragments from multiple housekeeping genes for the purpose of phylogenetic analysis, organism identification, and typing of species, strain, serovar, or other distinguishable phylogenetic level.
Miller Fisher Syndrome
A variant of the GUILLAIN-BARRE SYNDROME characterized by the acute onset of oculomotor dysfunction, ataxia, and loss of deep tendon reflexes with relative sparing of strength in the extremities and trunk. The ataxia is produced by peripheral sensory nerve dysfunction and not by cerebellar injury. Facial weakness and sensory loss may also occur. The process is mediated by autoantibodies directed against a component of myelin found in peripheral nerves. (Adams et al., Principles of Neurology, 6th ed, p1313; Neurology 1987 Sep;37(9):1493-8)
Turkeys
Large woodland game BIRDS in the subfamily Meleagridinae, family Phasianidae, order GALLIFORMES. Formerly they were considered a distinct family, Melegrididae.
Bacterial Shedding
The expelling of bacteria from the body. Important routes include the respiratory tract, genital tract, and intestinal tract.
Cattle
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Polymorphism, Restriction Fragment Length
Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.
Disease Reservoirs
Animate or inanimate sources which normally harbor disease-causing organisms and thus serve as potential sources of disease outbreaks. Reservoirs are distinguished from vectors (DISEASE VECTORS) and carriers, which are agents of disease transmission rather than continuing sources of potential disease outbreaks.
RNA, Ribosomal, 16S
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
DNA Fingerprinting
A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.
Water Microbiology
The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.
Helicobacter
A genus of gram-negative, spiral-shaped bacteria that has been isolated from the intestinal tract of mammals, including humans. It has been associated with PEPTIC ULCER.
Abortion, Septic
Any type of abortion, induced or spontaneous, that is associated with infection of the UTERUS and its appendages. It is characterized by FEVER, uterine tenderness, and foul discharge.
RNA, Ribosomal, 23S
Constituent of 50S subunit of prokaryotic ribosomes containing about 3200 nucleotides. 23S rRNA is involved in the initiation of polypeptide synthesis.
Seasons
Divisions of the year according to some regularly recurrent phenomena usually astronomical or climatic. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Antibodies, Bacterial
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Zoonoses
Diseases of non-human animals that may be transmitted to HUMANS or may be transmitted from humans to non-human animals.
Bacteroidaceae
A family of gram-negative bacteria found primarily in the intestinal tracts and mucous membranes of warm-blooded animals. Its organisms are sometimes pathogenic.
Cattle Diseases
Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.
Bacterial Adhesion
Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.
Nalidixic Acid
A synthetic 1,8-naphthyridine antimicrobial agent with a limited bacteriocidal spectrum. It is an inhibitor of the A subunit of bacterial DNA GYRASE.
Fluoroquinolones
A group of QUINOLONES with at least one fluorine atom and a piperazinyl group.
Antigens, Bacterial
Substances elaborated by bacteria that have antigenic activity.
Disease Outbreaks
Sudden increase in the incidence of a disease. The concept includes EPIDEMICS and PANDEMICS.
Virulence
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
Gastritis
Inflammation of the GASTRIC MUCOSA, a lesion observed in a number of unrelated disorders.
Milk
The white liquid secreted by the mammary glands. It contains proteins, sugar, lipids, vitamins, and minerals.
Drug Resistance, Microbial
The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
Genome, Bacterial
The genetic complement of a BACTERIA as represented in its DNA.
Anti-Infective Agents
Substances that prevent infectious agents or organisms from spreading or kill infectious agents in order to prevent the spread of infection.
Base Sequence
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Intestines
The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.
DNA, Ribosomal
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
Birds
Warm-blooded VERTEBRATES possessing FEATHERS and belonging to the class Aves.
Genotype
The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Molecular Mimicry
The structure of one molecule that imitates or simulates the structure of a different molecule.
Microbial Viability
Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate.
Meat Products
Articles of food which are derived by a process of manufacture from any portion of carcasses of any animal used for food (e.g., head cheese, sausage, scrapple).
Environmental Microbiology
The study of microorganisms living in a variety of environments (air, soil, water, etc.) and their pathogenic relationship to other organisms including man.
Travel
Aspects of health and disease related to travel.
Phylogeny
The relationships of groups of organisms as reflected by their genetic makeup.
Shigella
A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that ferments sugar without gas production. Its organisms are intestinal pathogens of man and other primates and cause bacillary dysentery (DYSENTERY, BACILLARY).
Molecular Epidemiology
The application of molecular biology to the answering of epidemiological questions. The examination of patterns of changes in DNA to implicate particular carcinogens and the use of molecular markers to predict which individuals are at highest risk for a disease are common examples.
Gene Expression Regulation, Bacterial
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.

A simple technique for mass cultivation of Campylobacter fetus. (1/1058)

Studies using 86 media for maximum growth of Campylobacter fetus for antigen production showed that a diphasic medium (solid base with liquid overlay) was most suitable. The solid base was double strength cystine heart agar. The liquid overlay was thioglycollate medium of Brewer (135-C) without agar. This medium yielded maximum growth of C. fetus in six days with good motility, less clumping and less filament formation than all other media tried.  (+info)

Detection of campylobacter in gastroenteritis: comparison of direct PCR assay of faecal samples with selective culture. (2/1058)

The prevalence of campylobacter gastroenteritis has been estimated by bacterial isolation using selective culture. However, there is evidence that certain species and strains are not recovered on selective agars. We have therefore compared direct PCR assays of faecal samples with campylobacter culture, and explored the potential of PCR for simultaneous detection and identification to the species level. Two hundred unselected faecal samples from cases of acute gastroenteritis were cultured on modified charcoal cefoperazone deoxycholate agar and subjected to DNA extraction and PCR assay. Culture on CCDA indicated that 16 of the 200 samples contained 'Campylobacter spp.'. By contrast, PCR assays detected campylobacters in 19 of the 200 samples, including 15 of the culture-positive samples, and further identified them as: C. jejuni (16), C. coli (2) and C. hyointestinalis (1). These results show that PCR offers a different perspective on the incidence and identity of campylobacters in human gastroenteritis.  (+info)

Presence of Campylobacter and Salmonella in sand from bathing beaches. (3/1058)

The purpose of this study was to determine the presence of thermophilic Campylobacter spp. and Salmonella spp. in sand from non-EEC standard and EEC standard designated beaches in different locations in the UK and to assess if potentially pathogenic strains were present. Campylobacter spp. were detected in 82/182 (45%) of sand samples and Salmonella spp. in 10/182 (6%). Campylobacter spp. were isolated from 46/92 (50%) of samples from non-EEC standard beaches and 36/90 (40%) from EEC standard beaches. The prevalence of Campylobacter spp. was greater in wet sand from both types of beaches but, surprisingly, more than 30% of samples from dry sand also contained these organisms. The major pathogenic species C. jejuni and C. coli were more prevalent in sand from non-EEC standard beaches. In contrast, C. lari and urease positive thermophilic campylobacters, which are associated with seagulls and other migratory birds, were more prevalent in sand from EEC standard beaches. Campylobacter isolates were further characterized by biotyping and serotyping, which confirmed that strains known to be of types associated with human infections were frequently found in sand on bathing beaches.  (+info)

Clonality of Campylobacter sputorum bv. paraureolyticus determined by macrorestriction profiling and biotyping, and evidence for long-term persistent infection in cattle. (4/1058)

Eighteen strains of Campylobacter sputorum bv. paraureolyticus (isolated over a 12-month period from seven dairy cows contained in a single herd) were examined by resistotyping, and macrorestriction profiling using pulsed field gel electrophoresis (PFGE). The resistotypes of these strains were identical, although repeat testing indicated resistance to metronidazole was not a reliable trait for typing purposes. Five SmaI-derived genotypes were identified among the 18 strains. In 5 of 7 cows, isolates obtained from the same animal, but from different time periods, were genotypically indistinguishable, indicating persistence of infection. Macrorestriction profiles of 5 strains representing the 5 SmaI genotypes and 8 other strains of C. sputorum from various sources, were prepared using 4 endonucleases (SmaI, SalI, BamHI and KpnI). The only other strain of C. sputorum bv. paraureolyticus examined (a Canadian isolate from human faeces), was found to have a SmaI macrorestriction profile identical with one of the five clones isolated from the cattle. Moreover, SalI and BamHI profiles of all bv. paraureolyticus strains were similar, while digestion with KpnI was not observed. By contrast, the seven strains of C. sputorum bv. sputorum yielded various macrorestriction profiles with all the enzymes used, and features distinguishing the two biovars studied could be identified. This study indicates that C. sputorum can persist in cattle for at least 12 months and exhibits a clonal population genetic structure.  (+info)

Detection of cytolethal distending toxin activity and cdt genes in Campylobacter spp. isolated from chicken carcasses. (5/1058)

This study was designed to determine whether isolates from chicken carcasses, the primary source of Campylobacter jejuni and Campylobacter coli in human infections, commonly carry the cdt genes and also whether active cytolethal distending toxin (CDT) is produced by these isolates. Campylobacter spp. were isolated from all 91 fresh chicken carcasses purchased from local supermarkets. Campylobacter spp. were identified on the basis of both biochemical and PCR tests. Of the 105 isolates, 70 (67%) were identified as C. jejuni, and 35 (33%) were identified as C. coli. PCR tests amplified portions of the cdt genes from all 105 isolates. Restriction analysis of PCR products indicated that there appeared to be species-specific differences between the C. jejuni and C. coli cdt genes, but that the restriction patterns of the cdt genes within strains of the same species were almost invariant. Quantitation of active CDT levels produced by the isolates indicated that all C. jejuni strains except four (94%) had mean CDT titers greater than 100. Only one C. jejuni strain appeared to produce no active CDT. C. coli isolates produced little or no toxin. These results confirm the high rate of Campylobacter sp. contamination of fresh chicken carcasses and indicate that cdt genes may be universally present in C. jejuni and C. coli isolates from chicken carcasses.  (+info)

Cloning and characterization of two bistructural S-layer-RTX proteins from Campylobacter rectus. (6/1058)

Campylobacter rectus is an important periodontal pathogen in humans. A surface-layer (S-layer) protein and a cytotoxic activity have been characterized and are thought to be its major virulence factors. The cytotoxic activity was suggested to be due to a pore-forming protein toxin belonging to the RTX (repeats in the structural toxins) family. In the present work, two closely related genes, csxA and csxB (for C. rectus S-layer and RTX protein) were cloned from C. rectus and characterized. The Csx proteins appear to be bifunctional and possess two structurally different domains. The N-terminal part shows similarity with S-layer protein, especially SapA and SapB of C. fetus and Crs of C. rectus. The C-terminal part comprising most of CsxA and CsxB is a domain with 48 and 59 glycine-rich canonical nonapeptide repeats, respectively, arranged in three blocks. Purified recombinant Csx peptides bind Ca2+. These are characteristic traits of RTX toxin proteins. The S-layer and RTX domains of Csx are separated by a proline-rich stretch of 48 amino acids. All C. rectus isolates studied contained copies of either the csxA or csxB gene or both; csx genes were absent from all other Campylobacter and Helicobacter species examined. Serum of a patient with acute gingivitis showed a strong reaction to recombinant Csx protein on immunoblots.  (+info)

Different invasion phenotypes of Campylobacter isolates in Caco-2 cell monolayers. (7/1058)

The pathogenesis of campylobacter enteritis is not well understood, but invasion into and translocation across intestinal epithelial cells may be involved in the disease process, as demonstrated for a number of other enteric pathogens. However, the mechanisms involved in these processes are not clearly defined for campylobacters. In this study, isolates were compared quantitatively in established assays with the enterocyte-like cell line, Caco-2, to determine the extent to which intracellular invasion contributes to translocation across epithelial cell monolayers, and whether isolates vary in this respect. Ten fresh Campylobacter isolates were compared and shown to differ in invasiveness by a factor of 10-fold by following their recovery from gentamicin-treated Caco-2 cells grown on nonpermeable tissue-culture wells. Four of these isolates with contrasting invasive ability were also shown to vary in their ability to translocate across Caco-2 cells grown on semipermeable Transwell inserts by a factor >10. However, translocation did not quantitatively correlate with the intracellular invasiveness of these isolates. Isolate no. 9752 was poorly invasive but had modest translocation ability, isolate no. 10392 was very invasive but did not translocate significantly and remained within the monolayer, isolate no. 9519 both translocated and invaded well, whereas, isolate no. 235 translocated very efficiently but was poorly invasive. Isolate no. 9519 also uniquely caused a transitory flattening of the Caco-2 cells and a possible drop in trans-epithelial electrical resistance (TEER) of the Transwell monolayers, whereas isolate no. 235 did not show these effects. Together these data demonstrate that there are significantly different 'invasion' phenotypes among Campylobacter strains involving different degrees of intracellular invasion, and either different rates of transcellular trafficking or, alternatively, paracellular trafficking.  (+info)

Rapid identification of thermotolerant Campylobacter jejuni, Campylobacter coli, Campylobacter lari, and Campylobacter upsaliensis from various geographic locations by a GTPase-based PCR-reverse hybridization assay. (8/1058)

Recently, a gene from Campylobacter jejuni encoding a putative GTPase was identified. Based on two semiconserved GTP-binding sites encoded within this gene, PCR primers were selected that allow amplification of a 153-bp fragment from C. jejuni, C. coli, C. lari, and C. upsaliensis. Sequence analysis of these PCR products revealed consistent interspecies variation, which allowed the definition of species-specific probes for each of the four thermotolerant Campylobacter species. Multiple probes were used to develop a line probe assay (LiPA) that permits analysis of PCR products by a single reverse hybridization step. A total of 320 reference strains and clinical isolates from various geographic origins were tested by the GTP-based PCR-LiPA. The PCR-LiPA is highly specific in comparison with conventional identification methods, including biochemical and whole-cell protein analyses. In conclusion, a simple method has been developed for rapid and highly specific identification of thermotolerant Campylobacter species.  (+info)

Phylogenetic Study of the Genus Campylobacter | Microbiology SocietyPhylogenetic Study of the Genus Campylobacter | Microbiology Society
The phylogenetic relationships of all species in the genus Campylobacter, Wolinella succinogenes, and other gram-negative bacteria were determined by comparison of partial 16S ribosomal ribonucleic acid sequences. The results of this study indicate that species now recognized in the genus Campylobacter make up three separate ribosomal ribonucleic acid sequence homology groups. Homology group I contains the following true Campylobacter species: Campylobacter fetus (type species), Campylobacter coli, Campylobacter jejuni, Campylobacter laridis, Campylobacter hyointestinalis, Campylobacter concisus, Campylobacter mucosalis, Campylobacter sputorum, and
https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-38-2-190
Occurrence and enumeration of Campylobacter spp . during the processing of Chilean broilers | BMC Microbiology | Full TextOccurrence and enumeration of Campylobacter spp . during the processing of Chilean broilers | BMC Microbiology | Full Text
Thermotolerant Campylobacter is among the more prevalent bacterial pathogens that cause foodborne diseases. This study aimed at evaluating the occurrence of thermotolerant Campylobacter contamination in chicken carcasses and processing plant stations (chilling water, scalding water, defeathering machinery, evisceration machine, and transport crates) in two of the Chilean main slaughterhouses. In addition, the isolation rates of thermotolerant Campylobacter during evisceration and following chiller processing were compared. The overall slaughterhouse contamination with thermotolerant Campylobacter was 54%. Differences were evident when the results from each plant were compared (plant A and plant B was 72% and 36%, respectively). The sampling points with the greatest contamination rates in both plants were after evisceration (90% and 54%, for plants A and B respectively). The decrease of thermotolerant Campylobacter contamination after chilling was significant (2 and 1.6 logs for plant A and B
https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-9-94
Campylobacter concisus Tanner et al. ATCC ® BAA-1457D-5™Campylobacter concisus Tanner et al. ATCC ® BAA-1457D-5™
Campylobacter concisus ATCC ® BAA-1457D-5™ Designation: Genomic DNA from Campylobacter concisus strain RM 5485 TypeStrain=False Application:
https://www.atcc.org/Products/All/BAA-1457D-5.aspx?p=1&rel=%7B0%7D
Concurrent Quantitation of Total Campylobacter and Total Ciprofloxacin-Resistant Campylobacter Loads in Rinses from Retail Raw...Concurrent Quantitation of Total Campylobacter and Total Ciprofloxacin-Resistant Campylobacter Loads in Rinses from Retail Raw...
One of the highest-priority research needs on Campylobacter was to develop laboratory methods for quantifying an antibiotic-resistant Campylobacter load persisting on raw poultry products to aid in risk assessment, to evaluate intervention strategies, and to develop meaningful baseline data for this pathogen. Currently, there is no published method for estimating loads of ciprofloxacin-resistant Campylobacter CFU within the total Campylobacter CFU load per chicken carcass. The recently published direct-plating method by U.S. Department of Agriculture (USDA)-Agricultural Resource Service (17, 18) permitted the quantitative enumeration of Campylobacter CFU but not of antibiotic-resistant Campylobacter. Ge et al. (12) recently examined the antimicrobial susceptibilities of 378 Campylobacter species isolates obtained by an enrichment method from retail meats, but their method did not permit quantitation of the numbers of such antibiotic-resistant Campylobacter present in those meat products. Stern ...
https://aem.asm.org/content/71/8/4510
Difference between revisions of Campylobacter ureolyticus - microbewiki
1) Vandamme P., Debruyne L., De Brandt E., & Falsen E. 2010. Reclassification of bacteroides ureolyticus as Campylobacter ureolyticus comb. nov., and emended description of the genus Campylobacter. International Journal of Systematic and Evolutionary Microbiology 60: 2016-2022. (2) Lui F., Ma R. N., Wang Y. M. , & Zhang L. 2018. The clinical importance of Campylobacter concisus and other human hosted Campylobacter species. Frontiers In Cellular And Infection Microbiology 8: 243. (3) Bullman, S., Lucid, A., Corcoran, D., Sleator, R. D., & Lucey, B. 2013. Genomic Investigation into Strain Heterogeneity and Pathogenic Potential of the Emerging Gastrointestinal Pathogen Campylobacter ureolyticus. PLoS ONE, 8:e71515. (4) ODonovan, D., Corcoran, G. D., Lucey, B., & Sleator, R. D. 2014. Campylobacter ureolyticus:A portrait of the pathogen. Virulence, 5:498-506. (5) Kaakush, N. O., Castano-Rodriguez, N., Mitchell, H. M., & Man, S. 2015. Global Epidemiology of Campylobacter Infection. Clinical ...
https://microbewiki.kenyon.edu/index.php?title=Campylobacter_ureolyticus&diff=prev&oldid=136479
Seasonal variation of thermophilic campylobacters in lambs at slaughter. - Lancaster EPrintsSeasonal variation of thermophilic campylobacters in lambs at slaughter. - Lancaster EPrints
A survey of the isolation rate and population size of thermophilic campylobacters in lambs at slaughter was carried out to determine the seasonal variation of thermophilic campylobacters in ovine hosts. Isolation rates determined by enrichment methods were always higher than those using direct plating onto selective agar and showed that Campylobacter could be isolated from 91·7% (n = 360) of samples from the small intestine of the lambs. Enumerations (MPN), done monthly over a 2-year period, averaged 4·00 log 10 (n = 1080, S.D. 0·16) campylobacters g−1 fresh weight (fw) intestinal contents with some samples giving values higher than 7 log MPN gfw−1. These results show that the prevalence of thermophilic campylobacters in sheep intended for slaughter is much higher than previously reported. Statistical analyses showed that there was a significant seasonal periodicity in the Campylobacter populations in the small intestines of lambs at slaughter (P = 0·004) but that there was no ...
https://eprints.lancs.ac.uk/id/eprint/19478/?template=browse
Campylobacter lanienae - WikipediaCampylobacter lanienae - Wikipedia
Campylobacter lanienae is a species of Campylobacter found in humans and other animals. Like other Campylobacter species, it is rod-shaped, non-glucose-fermenting, oxidase- and catalase-positive, Gram-negative and motile. Logan, J.; Burnens, A.; Linton, D.; Lawson, A. J.; Stanley, J. (2000). Campylobacter lanienae sp. nov., a new species isolated from workers in an abattoir. International Journal of Systematic and Evolutionary Microbiology. 50 (2): 865-872. doi:10.1099/00207713-50-2-865. ISSN 1466-5026. PMID 10758898. Lund, M.; Nordentoft, S.; Pedersen, K.; Madsen, M. (2004). Detection of Campylobacter spp. in Chicken Fecal Samples by Real-Time PCR. Journal of Clinical Microbiology. 42 (11): 5125-5132. doi:10.1128/JCM.42.11.5125-5132.2004. ISSN 0095-1137. Inglis GD, Kalischuk LD, Busz HW, Kastelic JP (September 2005). Colonization of cattle intestines by Campylobacter jejuni and Campylobacter lanienae. Applied and Environmental Microbiology. 71 (9): 5145-53. ...
https://en.wikipedia.org/wiki/Campylobacter_lanienae
Antimicrobial resistance and genomic screening of clinical isolates of thermophilic Campylobacter spp. from south-east...Antimicrobial resistance and genomic screening of clinical isolates of thermophilic Campylobacter spp. from south-east...
Aim: To screen 90 clinical isolates of thermophilic Campylobacter species for putative resistance to ampicillin, erythromycin and tetracycline and perform numerical analysis to determine isolate relatedness. Methods and Results: Disc diffusion, E-test MIC and agar dilution methods were performed. Disc diffusion testing showed 87 (97%) isolates appeared resistant to ampicillin at 10 姻 14 (16%) resistant to tetracycline at 30 姻 and three (3紥) resistant to erythromycin at 15 姮 E-test MICs showed a range of 0絠to ,256 mg l1 for ampicillin; 16 to ,256 mg l1 for tetracycline; and ,256 mg l1 for erythromycin. E-test showed 68% correlation (ᱠlog2 dilution) with agar dilution for ampicillin, 100% for erythromycin and 64% for tetracycline. Disc diffusion testing showed 100% correlation with agar dilution for erythromycin and tetracycline, and 77% for ampicillin. Numerical analyses of restriction endonuclease (RE) fragment profiles suggested a high level of isolate variation. Conclusion: The ...
https://research-repository.griffith.edu.au/handle/10072/6232
Download Campylobacter Pylori: Proceedings Of The First International Symposium On Campylobacter Pylori, Kronberg, June 12-13Th...Download Campylobacter Pylori: Proceedings Of The First International Symposium On Campylobacter Pylori, Kronberg, June 12-13Th...
With over a billion halves and download Campylobacter pylori: Proceedings of the First, talking Tom Cat 2 is hard with states and equivalents already over the Creator. m then + SimSimi Entertainment By: SimSimi Inc. SimSimi contains the public member depending buily. SimSimi s complete survey app Anytime, not you can face with SimSimi.
http://www.honeycuttlawoffices.com/guest/ebooks/download-Campylobacter-pylori%3A-Proceedings-of-the-First-International-Symposium-on-Campylobacter-pylori%2C-Kronberg%2C-June-12%E2%80%9313th%2C-1987-1988/
Bacteremia Due to Campylobacter Species: Clinical Findings and Antimicrobial Susceptibility Patterns : Clinical Infectious...
From 1979 to 1996, 58 patients (mean age, 39.4 years) were treated for bacteremia due to Campylobacter species at the Hospitals Vall dHebron in Barcelona, Spain. Bacteremia was considered to be hospital acquired in 30% of these patients. Almost all the patients (93%) had underlying conditions; liver cirrhosis was the most frequent (34% of patients), and neoplasia, immunosuppressive therapy, and human immunodeficiency virus disease were also common. Of the 58 Campylobacter strains isolated, 81% were C. jejuni, 10% were Campylobacter species, 7% were C. fetus, and one (2%) was C. coli. Resistance rates were: cephalothin, 82%; co-trimoxazole, 79%; quinolones, 54%; ampicillin, 20%; amoxicillin/clavulanate, 4%; erythromycin, 7%; gentamicin, 0; and tetracyclines, 0. Even though the majority of patients were immunocompromised, mortality was low (10.5%), and only one patient relapsed. Because of the high level of resistance to the quinolones in Campylobacter species, these drugs should not be used as ...
http://oxfordindex.oup.com/view/10.1086/516127
Emerging dynamics of human campylobacteriosis in Southern Ireland by Susan Bullman, Daniel Corcoran et al.Emerging dynamics of human campylobacteriosis in Southern Ireland by Susan Bullman, Daniel Corcoran et al.
Infections with Campylobacter spp. pose a significant health burden worldwide. The significance of Campylobacter jejuni/Campylobacter coli infection is well appreciated but the contribution of non-C. jejuni/C. coli spp. to human gastroenteritis is largely unknown. In this study, we employed a two-tiered molecular study on 7194 patient faecal samples received by the Microbiology Department in Cork University Hospital during 2009. The first step, using EntericBio® (Serosep), a multiplex PCR system, detected Campylobacter to the genus level. The second step, utilizing Campylobacter species-specific PCR identified to the species level. A total of 340 samples were confirmed as Campylobacter genus positive, 329 of which were identified to species level with 33 samples containing mixed Campylobacter infections. Campylobacter jejuni, present in 72.4% of samples, was the most common species detected, however, 27.4% of patient samples contained non-C. jejuni/C. coli spp.; Campylobacter fetus (2.4%),
https://sword.cit.ie/dptbiosciart/46/
Analiza trenda prevalencije infekcija uzrokovanih Salmonella species i Campylobacter species temeljem prijava oboljelih od...
Aim: To analyse the trend of prevalence of infections caused by Salmonella species and Campylobacter species. According to data based on the notification of infectious diseases collected at Teaching Institute for Public Health Primorsko-goranska County for a seven-year period from 2009 to 2015 we made analysis of the prevalence of infection caused by Salmonella species comparing to infection caused by Campylobacter species in Primorsko-goranska County. Examinees and methods: The study included mandatory diseases notification for infections caused by Salmonella species and Campylobacter species for the period from 2009 to 2015 in the Primorsko-goranska County . The data used in the study were obtained by the Teaching Institute for Public Health of Primorsko-goranska County that are not available to the public. The analysis was conducted based on the study of certain variables: the prevalence, sex, age , municipality, region, means of diagnosis, month of the year and occupation. Results: The ...
https://repository.medri.uniri.hr/islandora/object/medri:545
Analiza i usporedba kretanja infekcije Campylobacter species za Primorsko-goransku županiju i Grad Zagreb u razdoblju od 2011....
Kampilobakterioza je zoonoza uzrokovana rodom Campylobacter spp i vodeći je uročnik akutnog gastroenteritisa. U 80% infekcija za zarazu je odgovoran Campylobacter jejuni, u 10% zaraza je Campylobacter coli te u 0,1% Campylobacter lari. Jedini soj koji je dokazano nepatogen za čovjeka je Campylobacter jejuni suspp. doylei. Bakterija se prenosi najčešće putem hrane (goveda, svinje,perad), neklorirane vode te nepasteriziranog mlijeka. Uzrokuje infekciju kojoj su simptomi glavobolja, proljev, mučnina i grčevi koje u nekim slučajevima prati vrućica. Uspoređujući podatke sa zavoda za javno zdravstvo dviju županija, Primorsko-goranska županija i Grad Zagreb, ustanovljena je veća stopa incidencije u Primorsko-goranskoj županiji. Kampilobakterioza se češće javlja u ljetnim mjesecima dok se u prvom tromjesečju zabilježava niži broj slučajeva. U Republici Hrvatskoj od kampilobakterioze češće obolijevaju pripadnici muškog spola. U Primorsko-goranskoj županiji u razdoblju od ...
https://repository.medri.uniri.hr/islandora/object/medri%3A581
The interplay between Campylobacter and Helicobacter species and other gastrointestinal microbiota of commercial broiler...The interplay between Campylobacter and Helicobacter species and other gastrointestinal microbiota of commercial broiler...
Poultry represent an important source of foodborne enteropathogens, in particular thermophilic Campylobacter species. Many of these organisms colonize the intestinal tract of broiler chickens as harmless commensals, and therefore, often remain undetected prior to slaughter. The exact reasons for the lack of clinical disease are unknown, but analysis of the gastrointestinal microbiota of broiler chickens may improve our understanding of the microbial interactions with the host. In this study, the fecal microbiota of 31 market-age (56-day old) broiler chickens, from two different farms, was analyzed using high throughput sequencing. The samples were then screened for two emerging human pathogens, Campylobacter concisus and Helicobacter pullorum, using species-specific PCR. The gastrointestinal microbiota of chickens was classified into four potential enterotypes, similar to that of humans, where three enterotypes have been identified. The results indicated that variations between farms may have
https://gutpathogens.biomedcentral.com/articles/10.1186/1757-4749-6-18/tables/2
Genotyping and antibiotic resistance of thermophilic Campylobacter isolated from chicken and pig meat in Vietnam | Gut...Genotyping and antibiotic resistance of thermophilic Campylobacter isolated from chicken and pig meat in Vietnam | Gut...
Campylobacter species are recognized as the most common cause of foodborne bacterial gastroenteritis in humans. In this study nine Campylobacter strains isolated from chicken meat and pork in Hanoi, Vietnam, were characterized using molecular methods and tested for antibiotic resistance. The nine isolates (eight C. jejuni and one C. coli) were identified by multiplex PCR, and tested for the presence or absence of 29 gene loci associated with virulence, lipooligosaccharide (LOS) biosynthesis and further functions. flaA typing, multilocus sequence typing and microarray assay investigation showed a high degree of genetic diversity among these isolates. In all isolates motility genes (flaA, flaB, flhA, fliM), colonization associated genes (cadF, docB), toxin production genes (cdtA, cdtB, secD, secF), and the LOS biosynthesis gene pglB were detected. Eight gene loci (fliY, virB11, Cje1278, Cj1434c, Cj1138, Cj1438c, Cj1440c, Cj1136) could not be detected by PCR. A differing
https://gutpathogens.biomedcentral.com/articles/10.1186/s13099-016-0100-x
Comparison of different sampling types across the rearing period in broiler flocks for isolation of Campylobacter spp.Comparison of different sampling types across the rearing period in broiler flocks for isolation of Campylobacter spp.
EN] Campylobacter is the most common bacterial cause of human gastrointestinal disease in most developed countries. It is generally accepted that poultry products are a significant source of foodborne Campylobacter infections in humans. Assessing the effectiveness of any potential intervention at farm level requires monitoring of the Campylobacter status of broiler flocks, using appropriate sampling methods. The aim of this study was to assess the influence of the sample type across the rearing period for the detection of Campylobacter spp. at farm level. During this study, 21 commercial broiler farms were intensively sampled. Each farm was visited and sampled at different times during the rearing period (d 1, 7, 14, 21, 28, 35, and 42). On the first day of rearing, the status of the house and the day-old flock was evaluated, and environmental and cecal samples were collected. During rearing, 4 different sample types were collected: feces with sock swabs (sock swabs), feces directly from the ...
https://riunet.upv.es/handle/10251/65126
Risk factors and likelihood of campylobacter colonization in broiler flocks.Risk factors and likelihood of campylobacter colonization in broiler flocks.
Campylobacter was investigated in cecal droppings, feces, and cloacal swabs of 22 flocks of 3 to 5 week-old broilers. Risk factors and the likelihood of the presence of this agent in these flocks were determined. Management practices, such as cleaning and disinfection, feeding, drinkers, and litter treatments, were assessed. Results were evaluated using Odds Ratio (OR) test, and their significance was tested by Fishers test (p,0.05). A Campylobacter prevalence of 81.8% was found in the broiler flocks (18/22), and within positive flocks, it varied between 85 and 100%. Campylobacter incidence among sample types was homogenous, being 81.8% in cecal droppings, 80.9% in feces, and 80.4% in cloacal swabs (230). Flocks fed by automatic feeding systems presented higher incidence of Campylobacter as compared to those fed by tube feeders. Litter was reused in 63.6% of the farm, and, despite the lack of statistical significance, there was higher likelihood of Campylobacter incidence when litter was ...
http://www.lume.ufrgs.br/handle/10183/20582
Resistance in Campylobacter Species: Increased Resistance to Fluoroquinolones and Seasonal Variation : Clinical Infectious...
To identify epidemiological features of culture-proven campylobacter infections and to determine resistance rates, we conducted a 4-year demographic survey of culture-proven campylobacteriosis in one Dutch region. Examination of 24,435 fecal specimens revealed 1,315 cases of campylobacteriosis (5.4%). The ofloxacin-resistance rate among Campylobacter isolates increased from 11% to 29%. Resistance against tetracycline fluctuated between 7% and 15%, and resistance against erythromycin remained low. Resistance against fluoroquinolones was seasonally influenced, with relatively high rates during winter. We conclude that resistance of Campylobacter isolates to fluoroquinolones is still rising, probably because of the use of fluoroquinolones (enrofloxacin) in animal husbandry.. ...
http://oxfordindex.oup.com/view/10.1086/520447
Figure 1 - Integronlike Structures in Campylobacter spp. of Human and Animal Origin - Volume 6, Number 1-February 2000 -...
Resistance to antimicrobial agents used to treat severe Campylobacter spp. gastroenteritis is increasing worldwide. We assessed the antimicrobial resistance patterns of Campylobacter spp. isolates of human and animal origin. More than half (n = 32) were resistant to sulphonamide, a feature known to be associated with the presence of integrons. Analysis of these integrons will further our understanding of Campylobacter spp. epidemiology.
https://wwwnc.cdc.gov/eid/article/6/1/00-0109-f1
Microbiology Society Journals | Cytotoxic activity in broth-culture filtrates of Campylobacter pyloriMicrobiology Society Journals | Cytotoxic activity in broth-culture filtrates of Campylobacter pylori
Summary Broth-culture filtrates of Campylobacter pylori induced non-lethal cytopathic effects in vitro in 7 of 9 mammalian cell lines tested. Transmission electronmicroscopy revealed that the response consisted of intracellular vacuolisation. Intestine 407 cells were among the most responsive and were used for routine assay. About 55% of isolates of C. pylori tested, originating from four geographic regions worldwide, produced cytotoxic activity. The activity was neutralisable by specific antisera to broth-culture filtrates or to sonicated bacteria but not by antisera to other bacterial preparations. Cytotoxic activity was heat-labile (70°C for 30 min), was protease-sensitive and ammonium-sulphate precipitable. It did not pass through an ultrafiltration membrane with a nominal mol.-wt limit of 100 x 103. It was concluded that C. pylori can produce a factor that alters cultured cells in vitro. The relevance of this factor to the pathogenesis of gastritis associated with C. pylori remains to be
http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-26-2-93
Effect of a Campylobacter pylori protein on acid secretion by parietal cellsEffect of a Campylobacter pylori protein on acid secretion by parietal cells
The uptake of [14C]-aminopyrine by rabbit gastric epithelial cells was used as an indirect assay for acid secretion from parietal cells. Campylobacter pylori strains, isolated from the stomachs of 3 patients with chronic gastritis, duodenal ulcer, and near-normal mucosa, respectively, inhibited acid …
https://pubmed.ncbi.nlm.nih.gov/2568521/
Research - Campylobacter Contamination in Fresh Whole Chilled UK -Produced Chickens at Retail - Jan-March 2017 | FoodWorldResearch - Campylobacter Contamination in Fresh Whole Chilled UK -Produced Chickens at Retail - Jan-March 2017 | FoodWorld
Food.gov Campylobacter contamination in fresh whole chilled UK-produced chickens at retail: January-March 2017 Key Results  The latest results show that in January-March 2017, 6.5% of chickens had high levels of Campylobacter (over 1000 cfu/g), down from 9.3% over the same period the previous year.  To compare the proportion of chickens with levels of…
https://kswfoodmicro.com/2017/08/01/research-campylobacter-contamination-in-fresh-whole-chilled-uk-produced-chickens-at-retail-jan-march-2017/
Determinants of survival and virulence of Campylobacter - Open Research OnlineDeterminants of survival and virulence of Campylobacter - Open Research Online
The pathogenesis of Campylobacter enteritis is not well understood including the mechanisms involved in invasion and translocation across intestinal epithelial cells. The genetic make-up of the pathogen and its responses to different environmental cues are thought to contribute to the organisms ability to survive and cause disease. The extremes of environment which Campylobacter can with-stand, and the effect that this has on virulence and invasive ability remains undefined. For the first time, several isolates were compared quantitatively to determine the extent to which intracellular invasion contributes to translocation across epithelial cell mono layers. Translocation ability did not correlate with intracellular invasiveness, suggesting that different invasion phenotypes exist among Campylobacter isolates. Repeated exposure of Campylobacter isolates to Caco-2 cells caused an increase in their ability to invade and survive, which was associated with changes in protein expression. ...
https://oro.open.ac.uk/58052/
IBIMA Publishing Identification of Human and Poultry Campylobacter Sequence Types in Small Indian Mongooses ...IBIMA Publishing Identification of Human and Poultry Campylobacter Sequence Types in Small Indian Mongooses ...
Introduction. Campylobacter is one of most common causative agents of bacterial food and waterborne illness in humans (Sheppard et al., 2009) and is responsible for an estimated 2.4 million cases of human illness per year in the US alone (Thomas et al., 1999; Center for Disease Control and Prevention, (CDC), National Center for Emerging Zoonotic and Infectious Diseases, 2010). Campylobacter is a gram negative, non-spore forming, rod-shaped bacterium and the genus is comprised of at least 17 species. Most Campylobacter species are microaerophilic, and most pathogenic species require thermophilic conditions for growth. The most common species affecting humans is C. jejuni. However, among several other species, C. coli,C. lariand C. ureolyticus have also been implicated in human infection (Thomas et al., 1999; Allos, 2001; Koziel et al., 2012). Poultry are considered a major source of human infection; however, other livestock such as cattle, sheep and pigs have also been identified as potential ...
http://ibimapublishing.com/articles/IJVMR/2014/676408/
Identification of Transmission Routes of Campylobacter and On-Farm Measures to Reduce Campylobacter in Chicken - ARKdb-chicken...Identification of Transmission Routes of Campylobacter and On-Farm Measures to Reduce Campylobacter in Chicken - ARKdb-chicken...
An in-depth analysis was performed on Swedish broiler producers that had delivered chickens with Campylobacter to slaughter over several years, in order to identify possible transmission routes and formulate effective measures to prevent chickens being colonized with Campylobacter. Between 2017 and 2019, 626 samples were collected at farm level and Campylobacter was isolated from 133 (21.2%). All C. jejuni and C. coli isolated from these samples were whole-genome sequenced, together with isolates from the corresponding cecum samples at slaughter (n = 256). Core genome multi-locus sequence typing (cgMLST) analysis, using schemes consisting of 1140 and 529 genes for C. jejuni and C. coli, respectively, revealed that nearby cattle, contaminated drinking water, water ponds, transport crates, and parent flocks were potential reservoirs of Campylobacter. ...
http://thearkdb.org/identification-of-transmission-routes-of-campylobacter-and-on-farm-measures-to-reduce-campylobacter-in-chicken/
Intestinal carriage of verocytotoxigenic Escherichia coli O157, Salmonella, thermophilic Campylobacter and Yersinia...Intestinal carriage of verocytotoxigenic Escherichia coli O157, Salmonella, thermophilic Campylobacter and Yersinia...
Milnes, AS, Stewart, I, Clifton-Hadley, FA, Davies, RH, Newell, DG, Sayers, AR, Cheasty, T, Cassar, C, Ridley, A, Cook, AJC et al, Evans, SJ, Teale, CJ, Smith, RP, McNnally, A, Toszeghy, M, Futter, R, Kay, A and Paiba, GA. (2008) Intestinal carriage of verocytotoxigenic Escherichia coli O157, Salmonella, thermophilic Campylobacter and Yersinia enterocolitica, in cattle, sheep and pigs at slaughter in Great Britain during 2003 ...
http://epubs.surrey.ac.uk/825265/
em|Campylobacter|/em|: What you need to know | Jackson Hospitalem|Campylobacter|/em|: What you need to know | Jackson Hospital
Tiny and shaped like spirals, Campylobacter bacteria look pretty cool under a microscope. But you wouldnt want to invite them to a barbecue.. Unfortunately, however, thats what many of us do. And we end up feeling lousy as a result.. Along with salmonella and E. coli, Campylobacter bacteria are one of the most common causes of foodborne illness. But theres much we can do to protect ourselves from getting sick. How Campylobacter bacteria spread. Campylobacter are commonly found in the digestive tracts of cats, dogs, poultry, cattle and other animals, including humans, reports the U.S. Department of Agriculture (USDA). According to the Centers for Disease Control and Prevention (CDC), the most common way to get sick from Campylobacter is by eating undercooked chicken or foods contaminated by the juices of raw chicken. You also can get sick by eating undercooked meat, drinking unpasteurized milk, or by eating or drinking food or water thats been contaminated by the feces of infected animals. ...
https://www.jackson.org/health-library/story/fc9a8755-348b-4c47-9d7c-080761f72491
På vej mod vaccination mod campylobacter i fjerkræ - DTU FødevareinstituttetPå vej mod vaccination mod campylobacter i fjerkræ - DTU Fødevareinstituttet
Det konkrete pc-værktøj er udviklet i softwaret HUGIN, og her integreres viden om forskellige kontrolmetoders effekt og omkostninger. Værktøjet kan bruges på både besætnings- og flokniveau og integrerer betydningen af årstiden i forhold til forekomsten af campylobacter med tilstedeværelsen af for eksempel fluenet, øvrige vaccinationer og historisk viden om tidligere infektioner mod campylobacter i besætningen.. På baggrund heraf beregner værktøjet sandsynligheden for forskellige mængder af campylobacter i fjerkræflokken på slagtetidspunktet ved brug af forskellige kontrolmetoder. Efterfølgende sammenligner modellen omkostningerne ved forskellige kontrolmetoder med det forventede merafkast for slagteklare kyllinger med en lavere forekomst af campylobacter. Afhandlingen giver desuden en omfattende gennemgang af bekæmpelsesstrategier for campylobacter i hele fødevarekæden.. ...
https://www.food.dtu.dk/nyheder/Nyhed?id=%7B7B3813A2-C21D-445C-9282-528246BB77BD%7D
Campylobacteriosis - intestinal infection, body, causes, What Is Campylobacter?Campylobacteriosis - intestinal infection, body, causes, What Is Campylobacter?
Campylobacter (kamp-pi-lo-BAK-ter) is a type of bacteria that is a normal inhabitant of the digestive tract of many animals. People, however, do not normally carry Campylobacter, and exposure to it usually causes an intestinal infection called campylobacteriosis (kamp-pi-lo-bak-ter-ee-O-sis). The most common source of Campylobacter in the United States is chicken. When chickens (and other animals) are killed for food, the bacteria from their digestive tract can contaminate the meat. People get infected when they eat raw or uncooked meats and eggs (thorough cooking kills the bacteria), drink raw (unpasteurized) milk, or drink contaminated water. Oftentimes, juices from raw meats drip and contaminate other foods. In rare cases, contact with people or animals who are infected spreads the illness. Campylobacter is the most common bacterial cause of diarrhea in the United States, where more than 2 million cases occur each year. The illness most frequently affects infants and children younger than age ...
http://www.humanillnesses.com/original/At-Ca/Campylobacteriosis.html
Phs 398 (rev. 5/01), biographical sketch format page
Dr. Taylors research on Campylobacter species, particularly antibiotic resistance, DNA transformation and vector construction, have advanced Campylobacter genetics and enabled others using techniques developed in her laboratory, to make major contributions to understanding Campylobacter pathogenesis. Dr. Taylor was the first to demonstrate that Campylobacter jejuni and Campylobacter coli harbored tetracycline resistance (TcR) plasmids, which were transmissible only within the Campylobacter species, but not to other species such as Escherichia coli. She has spent the past 30 years investigating the novel mechanism of TcR, initially identified on Campylobacter plasmids and called Tet(O) but now recognized as an extremely common cause of TcR. Her group coined the term ribosomal protection for this mode of resistance, determined its gene sequence and similarity to translocation factors EF-G and EF-Tu. Recently her group showed that the Tet(O) protein modifies the ribosome by changing its ...
http://pdfpharm.com/a/aihealthsolutions.ca1.html
Researchers Demonstrate Potential Routes of Transmission of Salmonella and Campylobacter in Turkeys | The Poultry SiteResearchers Demonstrate Potential Routes of Transmission of Salmonella and Campylobacter in Turkeys | The Poultry Site
Sponsored by the US Poultry & Egg Association Harold E. Ford Foundation, the project included growing and monitoring of turkey breeder hens and toms to 65 weeks of age, artificial insemination and collection of fertile eggs for hatching a second-generation meat bird flock, and then monitoring these progeny (meat bird flock). Intervention assessments included washing fertile eggs with sanitiser and feeding probiotics to both breeder hens and meat bird progeny.. Monitoring results showed that Campylobacter spread rapidly and cross-contaminated turkeys throughout the grow-out house. For both Salmonella and Campylobacter, wild strains that appeared seemed to out-compete marker strains after a few weeks and persist in the flock.. The most common wild strains were Campylobacter jejuni (tetracycline-resistant), Campylobacter coli (kanamycin-resistant) and Salmonella Agona.. Pathogens were also isolated from pest vectors (flies, beetles and a rodent) in the houses, confirming the importance of proper ...
https://www.thepoultrysite.com/articles/researchers-demonstrate-potential-routes-of-transmission-of-salmonella-and-campylobacter-in-turkeys
Campylobacter Supplement (Boltons) | NEOGENCampylobacter Supplement (Boltons) | NEOGEN
Campylobacter Supplement (Boltons) is used with Campylobacter Enrichment Broth (Boltons) for the selective enrichment of Campylobacter spp. in food and water samples. Campylobacter Supplement (Boltons) is not intended for use in the diagnosis of disease or other conditions in humans ...
https://www.neogen.com/categories/microbiology/campylobacter-supplement-boltons/?recommendationId=1251228094801
Different invasion phenotypes of Campylobacter isolates in Caco-2 cell monolayers | Microbiology SocietyDifferent invasion phenotypes of Campylobacter isolates in Caco-2 cell monolayers | Microbiology Society
Summary The pathogenesis of campylobacter enteritis is not well understood, but invasion into and translocation across intestinal epithelial cells may be involved in the disease process, as demonstrated for a number of other enteric pathogens. However, the mechanisms involved in these processes are not clearly defined for campylobacters. In this study, isolates were compared quantitatively in established assays with the enterocyte-like cell line, Caco-2, to determine the extent to which intracellular invasion contributes to translocation across epithelial cell monolayers, and whether isolates vary in this respect. Ten fresh Campylobacter isolates were compared and shown to differ in invasiveness by a factor of 10-fold by following their recovery from gentamicin-treated Caco-2 cells grown on non-permeable tissue-culture wells. Four of these isolates with contrasting invasive ability were also shown to vary in their ability to translocate across Caco-2 cells grown on semi-permeable Transwell inserts by a
https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-48-5-461
Prevalence of Salmonella and Campylobacter in retail chicken carcasses in Senegal - Publications des agents du CiradPrevalence of Salmonella and Campylobacter in retail chicken carcasses in Senegal - Publications des agents du Cirad
Entre janvier 2001 et octobre 2002, 300 carcasses de poulets achet es chez des d taillants Dakar ont t examin es afin de d terminer la pr valence de Salmonella et de Campylobacter sur ce type daliment. Parmi les carcasses, 146 taient des produits frais, 58 des produits r frig r s et 96 des produits congel s. Salmonella a t isol e dans 96 (32 p. 100) carcasses. Salmonella Hadar (41,6 p. 100) et Salmonella Brancaster (20,8 p. 100) ont repr sent les s rovars pr dominants. Campylobacter spp. a t isol dans 168 (56 p. 100) carcasses. C. jejuni a t plus fr quemment identifi (59 p. 100) que C. coli (27 p. 100). Les taux de contamination pour Campylobacter ont t significativement diff rents en fonction de la temp rature de conservation des carcasses : cette bact rie a t effectivement isol e dans 76 p. 100 des carcasses conserv es temp rature ambiante, dans 53 p. 100 de celles r frig r es et dans 28 p. 100 de celles congel es. (R sum dauteur ...
http://publications.cirad.fr/une_notice.php?dk=519928
Diagnosis and Antimicrobial Susceptibility of Campylobacter species<...Diagnosis and Antimicrobial Susceptibility of Campylobacter species<...
TY - CHAP. T1 - Diagnosis and Antimicrobial Susceptibility of Campylobacter species. AU - Nachamkin, I.. AU - Engberg, J.. AU - Aarestrup, Frank Møller. A2 - Nachamkin, I.. A2 - Blaser, M. J.. PY - 2000. Y1 - 2000. M3 - Book chapter. SP - 45. EP - 66. BT - Campylobacter. PB - ASM Press. CY - Washington DC, USA. ER - ...
https://orbit.dtu.dk/en/publications/diagnosis-and-antimicrobial-susceptibility-of-campylobacter-speci
Journal of Water and HealthJournal of Water and Health
To study whether broiler and layer farms contribute to the environmental Campylobacter load, environmental matrices at or close to farms, and caecal material from chickens, were examined. Similarity between Campylobacter from poultry and environment was tested based on species identification and Multilocus Sequence Typing. Campylobacter prevalence in caecal samples was 97% at layer farms (n = 5), and 93% at broiler farms with Campylobacter-positive flocks (n = 2/3). Campylobacter prevalence in environmental samples was 24% at layer farms, and 29% at broiler farms with Campylobacter-positive flocks. Campylobacter was detected in soil and surface water, not in dust and flies. Campylobacter prevalence in adjacent and remote surface waters was not significantly (P , 0.1) different. Detected species were C. coli (52%), C. jejuni (40%) and C. lari (7%) in layers, and C. jejuni (100%) in broilers. Identical sequence types (STs) were detected in caecal material and soil. A deviating species distribution ...
http://jwh.iwaponline.com/content/early/2017/09/14/wh.2017.119
Vaccinating Chickens Against Campylobacter | Food Poisoning BulletinVaccinating Chickens Against Campylobacter | Food Poisoning Bulletin
A scientist who presented a paper at the Society for General Microbiologys spring conference in Dublin is developing a vaccine that could prevent Campylobacter in chickens. The research is being conducted at Washington State University.. Professor Michael Konkel, who is leading the research, is studying maternal antibodies that are naturally passed from hens to their chicks. The researchers have found the bacterial molecules the antibodies attack and are using them as a starting point for a vaccine.. Food poisoning caused by Campylobacter is quite common. In fact, this bacteria is the second most common cause of foodborne illness. A recent study at the University of Aberdeen found that 81% of chicken livers purchased at grocery stores in the UK contain Campylobacter.. A report called Ranking the Risks found that the food and bacteria combination that causes the most foodborne illness with the largest cost burden is Campylobacter in poultry.. Researchers and scientists havent been successful at ...
https://foodpoisoningbulletin.com/2012/vaccinating-chickens-against-campylobacter/
Table A1 - Fly Transmission of Campylobacter - Volume 11, Number 3-March 2005 - Emerging Infectious Disease journal - CDC
Campylobacter is common in birds. Migratory birds result in large seasonal changes in the inputs to the environment from bird feces and could contribute to human Campylobacter exposure (55). Migratory birds could be a seasonally changing driver to human disease (56). The main likely exposure route if this were the case would be direct contact with contaminated bird feces in the garden, contamination of field-grown fruit and vegetables and contamination of source waters for drinking. Bird-pecked milk is a recognized route by which Campylobacter infection can be acquired (53,54). The contamination is thought to result from birds feeding consecutively on cow feces and milk in bottles. The infections related to bird-pecked milk appear to be seasonal in distribution with a marked increase in May (57 ...
https://wwwnc.cdc.gov/eid/article/11/3/04-0460-ta1
Research - Development and evaluation of an on-farm rapid biosensor method for Campylobacter spp. detection | Harper Adams...
Routine detection of Campylobacter typically involves a 48 hour cultivation by selective enrichment under specific growth conditions, followed by detection using selective agar plating (requiring a further 48 hours of incubation followed by confirmation testing). At present, there are no rapid and sensitive in situ Campylobacter tests available. The use of a lateral flow device (LFD) can significantly reduce the result time but LFDs do not have sufficient sensitivity to be used for environmental detection without preliminary (48 hour) enrichment. This is therefore not suitable as a rapid in situ test. Campylobacter can also be detected using PCR-based methods but requires that the sample undergoes extensive preparation to extract the DNA and remove the environmental contaminants that interfere with the PCR reactions. Further, should the amplicon (part of the DNA that is detected and is amplified as part of the process) escape into the environment, the area becomes contaminated, giving false ...
https://www.harper-adams.ac.uk/research/project.cfm?id=101
Research Publications - Bacterial Pathogens - Campylobacter speciesResearch Publications - Bacterial Pathogens - Campylobacter species
Browse through articles from peer-reviewed research publications that focus on the Campylobacter species bacterial pathogen in food safety research.
https://www.nal.usda.gov/fsrio/research/publications/bacterial-pathogens/campylobacter?page=16
Campylobacter pylori y patolog a gastroduodenal - Alberto Ram rez-Ramos - Google BooksCampylobacter pylori y patolog a gastroduodenal - Alberto Ram rez-Ramos - Google Books
books.google.com.pehttps://books.google.com.pe/books/about/Campylobacter_pylori_y_patolog%C3%ADa_gastro.html?id=i7BtHAAACAAJ&utm_source=gb-gplus-shareCampylobacter pylori y patolog a gastroduodenal ...
https://books.google.com.pe/books/about/Campylobacter_pylori_y_patolog%C3%ADa_gastro.html?id=i7BtHAAACAAJ&hl=es-419
Identification of Campylobacter Heat-Stable and Heat-Labile Antigens by Combining the Penner and Lior Serotyping Schemes |...Identification of Campylobacter Heat-Stable and Heat-Labile Antigens by Combining the Penner and Lior Serotyping Schemes |...
A number of investigators in a variety of countries have compared these two serotyping protocols in terms of the strengths and advantages of each and what they have achieved since their introduction; however, it is difficult to compare O antigen typing results to those of HL antigen typing given that each scheme is used to detect different antigens on the bacterium. In 1985, Patton et al. (21) performed a comparative study using the Penner and Lior methods for serotyping Campylobacter. Their findings indicated that 96.1% of isolates were typeable by the Penner method and that 92.1% were typeable by the Lior method. In 1993, a second study by the same group determined that, of a representative sample of 298 Campylobacter isolates from across the United States, a total of 24 O antisera were needed to serotype 84.6% of the strains by the HS scheme (20). Among the most common serotypes were O:1 (or O:1,8), O:13,16,43,50, O:8 (or O:8,17), O:4, O:5−,5+, O:2, O:3, O:6,7,25,29, O:19, and O:15,38. One ...
https://jcm.asm.org/content/40/3/741?ijkey=394fd902a0c431eb5ee9452987ec045a9a78f4c0&keytype2=tf_ipsecsha
Pediatric Campylobacter Infections Differential DiagnosesPediatric Campylobacter Infections Differential Diagnoses
The family Campylobacteraceae includes 2 genera: Campylobacter and Arcobacter. The genus Campylobacter includes 18 species and subspecies; 11 of these are considered pathogenic to humans and cause enteric and extraintestinal illnesses.
https://emedicine.medscape.com/article/970552-differential
De Vries, J.J., Arents, N.L. and Manson, W.L. (2008) Campylobacter Species Isolated from Extra-Oro-Intestinal Abscesses A...De Vries, J.J., Arents, N.L. and Manson, W.L. (2008) Campylobacter Species Isolated from Extra-Oro-Intestinal Abscesses A...
De Vries, J.J., Arents, N.L. and Manson, W.L. (2008) Campylobacter Species Isolated from Extra-Oro-Intestinal Abscesses A Report of Four Cases and Literature Review. European Journal of Clinical Microbiology & Infectious Diseases, 27, 1119-1123.
http://www.scirp.org/reference/ReferencesPapers.aspx?ReferenceID=1096961
Chapter 155. Infections Due to Campylobacter and Related Organisms | Harrisons Principles of Internal Medicine, 18e |...Chapter 155. Infections Due to Campylobacter and Related Organisms | Harrisons Principles of Internal Medicine, 18e |...
pathogen is C. jejuni, which accounts for 80-90% of all cases of recognized illness due to campylobacters and related genera. Other organisms that cause diarrheal disease include C. coli, C. upsaliensis, C. lari, C. hyointestinalis, C. fetus, A. butzleri, A. cryaerophilus, H. cinaedi, and H. fennelliae. The two Helicobacter species causing diarrheal disease, H. cinaedi and H. fennelliae, are intestinal rather than gastric organisms; in terms of the clinical features of the illnesses they cause, these species most closely resemble Campylobacter rather than H. pylori (Chap. 151) and thus are considered in this chapter. ...
http://accesspharmacy.mhmedical.com/content.aspx?bookid=331§ionid=40726905
Campylobacter IndonesiaCampylobacter Indonesia
Campylobacteriosis is an illness that is caused by the bacteria of the genus Campylobacter. Most of the disease is caused by the species called campylobacter jejuni. The symptoms of this disease is diarrhea, cramps and fever within 2-5 days of infection. The diarrhea can be bloody with nausea and vomiting. This bacteria is gram negative and has a spiral shape. This disease is one of the most common cause of diarrheal outbreaks in Indonesia though it usually do not cause death and seldom reported. The most commonly reported cases of campylobacteriosis are associated with the eating of raw meat or under cooked meat or from cross contamination of other foods from these items ...
http://www.arasains.co.id/product_43_Deteksi-Campylobacter-Indonesia
Prevalence, antibiotic susceptibility, and genetic diversity of Salmonella, Campylobacter, and Escherichia coli O157:H7...Prevalence, antibiotic susceptibility, and genetic diversity of Salmonella, Campylobacter, and Escherichia coli O157:H7...
Salmonella, Campylobacter, and Eschericia coli O157:H7 are important foodborne pathogens, but longitudinal studies of their prevalence in beef cattle feedyards have not been done. Our long- term study involved 24,556 samples taken from beef cattle feedyards found overall prevalences of 4.87% for Salmonella, 20.1% for Campylobacter in hospital pen fecal samples, and 0.20% for E. coli O157:H7. Yard and pen differences (P,0.05) were detected. All 53 E. coli O157:H7 isolates were resistant to Talmicosin and Erythromycin, two antimicrobials used in food animal medicine. Their genetic diversity was high and did not indicate the presence of resident strains at the yards studied. Salmonella, Campylobacter, and E. coli O157:H7 were probably brought into the yards by shipments of new cattle. Many of these organismswere susceptible to antibiotics commonly used to treat beef cattle ...
http://krex.k-state.edu/dspace/handle/2097/4780
PigProgress - Campylobacter food poisoning - good news for pigs!PigProgress - Campylobacter food poisoning - good news for pigs!
Campylobacter species are the commonest cause of food poisoning in man, affecting over 50,000 people in the UK and 69/100,000 population in Denmark each year, but Danish researchers have shown that potentially, only a small fraction of pig isolates are associated with man and these strains are also found in poultry, which appears to be a major source.
http://www.pigprogress.net/Home/General/2008/1/Campylobacter-food-poisoning---good-news-for-pigs-PP002091W/
Toxin production by Campylobacter spp. | Clinical Microbiology ReviewsToxin production by Campylobacter spp. | Clinical Microbiology Reviews
Of all the virulence factors that were proposed for Campylobacter jejuni and related species to cause disease in humans, the discovery of toxin production was the most promising but led to a rather confusing and even disappointing stream of data. The discussion of whether proteinaceous exotoxins are relevant in disease remains open. One important reason for this lack of consensus is the anecdotal nature of the literature reports. To provide a basis for an unbiased opinion, this review compiles all described exotoxins, compares their reported properties, and provides a summary of animal model studies and clinical data. The toxins are divided into enterotoxins and cytotoxins and are sorted according to their biochemical properties. Since many Campylobacter toxins have been compared with toxins of other species, some key examples of the latter are also discussed. Future directions of toxin research that appear promising are defined. ...
https://cmr.asm.org/content/10/3/466
University of Hawaii Authors Examine Campylobacter On The Islands | Campylobacter BlogUniversity of Hawaii Authors Examine Campylobacter On The Islands | Campylobacter Blog
Hawaiis Star-Bulletin today takes a look at the high rate of campylobacter contamination being experienced on the islands of the nations 50th State.
http://www.campylobacterblog.com/campylobacter-outbreak/university-of-hawaii-authors-examine-campylobacter-on-the-islands/

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