AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
CalreticulinCalnexinRibonucleoproteinsCalcium-Binding ProteinsEndoplasmic ReticulumMolecular ChaperonesCalsequestrinProtein Disulfide-IsomerasesIndolizinesIsomerasesProtein FoldingLectinsMolecular Sequence DataAmino Acid SequenceCalbindin 2Protein BindingTrypanosoma cruziChagas DiseaseAdenocarcinomaMammary Neoplasms, ExperimentalMammary Tumor Virus, Mouse
Calreticulin
A multifunctional protein that is found primarily within membrane-bound organelles. In the ENDOPLASMIC RETICULUM it binds to specific N-linked oligosaccharides found on newly-synthesized proteins and functions as a MOLECULAR CHAPERONE that may play a role in PROTEIN FOLDING or retention and degradation of misfolded proteins. In addition calreticulin is a major storage form for CALCIUM and functions as a calcium-signaling molecule that can regulate intracellular calcium HOMEOSTASIS.
Calnexin
A lectin found in ENDOPLASMIC RETICULUM membranes that binds to specific N-linked OLIGOSACCHARIDES found on newly synthesized proteins. It may play role in PROTEIN FOLDING or retention and degradation of misfolded proteins in the endoplasmic reticulum.
Ribonucleoproteins
Complexes of RNA-binding proteins with ribonucleic acids (RNA).
Calcium-Binding Proteins
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
Endoplasmic Reticulum
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
Molecular Chaperones
A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.
Calsequestrin
Acidic protein found in SARCOPLASMIC RETICULUM that binds calcium to the extent of 700-900 nmoles/mg. It plays the role of sequestering calcium transported to the interior of the intracellular vesicle.
Protein Disulfide-Isomerases
Sulfur-sulfur bond isomerases that catalyze the rearrangement of disulfide bonds within proteins during folding. Specific protein disulfide-isomerase isoenzymes also occur as subunits of PROCOLLAGEN-PROLINE DIOXYGENASE.
Indolizines
Isomerases
A class of enzymes that catalyze geometric or structural changes within a molecule to form a single product. The reactions do not involve a net change in the concentrations of compounds other than the substrate and the product.(from Dorland, 28th ed) EC 5.
Protein Folding
Processes involved in the formation of TERTIARY PROTEIN STRUCTURE.
Lectins
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Amino Acid Sequence
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Calbindin 2
A calbindin protein that is differentially expressed in distinct populations of NEURONS throughout the vertebrate and invertebrate NERVOUS SYSTEM, and modulates intrinsic neuronal excitability and influences LONG-TERM POTENTIATION. It is also found in LUNG, TESTIS, OVARY, KIDNEY, and BREAST, and is expressed in many tumor types found in these tissues. It is often used as an immunohistochemical marker for MESOTHELIOMA.
Protein Binding
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Trypanosoma cruzi
The agent of South American trypanosomiasis or CHAGAS DISEASE. Its vertebrate hosts are man and various domestic and wild animals. Insects of several species are vectors.
Chagas Disease
Infection with the protozoan parasite TRYPANOSOMA CRUZI, a form of TRYPANOSOMIASIS endemic in Central and South America. It is named after the Brazilian physician Carlos Chagas, who discovered the parasite. Infection by the parasite (positive serologic result only) is distinguished from the clinical manifestations that develop years later, such as destruction of PARASYMPATHETIC GANGLIA; CHAGAS CARDIOMYOPATHY; and dysfunction of the ESOPHAGUS or COLON.
Adenocarcinoma
A malignant epithelial tumor with a glandular organization.
Mammary Neoplasms, Experimental
Experimentally induced mammary neoplasms in animals to provide a model for studying human BREAST NEOPLASMS.
Mammary Tumor Virus, Mouse
The type species of BETARETROVIRUS commonly latent in mice. It causes mammary adenocarcinoma in a genetically susceptible strain of mice when the appropriate hormonal influences operate.

Calreticulin, a peptide-binding chaperone of the endoplasmic reticulum, elicits tumor- and peptide-specific immunity. (1/690)

Calreticulin (CRT), a peptide-binding heat shock protein (HSP) of the endoplasmic reticulum (ER), has been shown previously to associate with peptides transported into the ER by transporter associated with antigen processing (Spee, P., and J. Neefjes. 1997. Eur. J. Immunol. 27: 2441-2449). Our studies show that CRT preparations purified from tumors elicit specific immunity to the tumor used as the source of CRT but not to an antigenically distinct tumor. The immunogenicity is attributed to the peptides associated with the CRT molecule and not to the CRT molecule per se. It is further shown that CRT molecules can be complexed in vitro to unglycosylated peptides and used to elicit peptide-specific CD8(+) T cell response in spite of exogenous administration. These characteristics of CRT closely resemble those of HSPs gp96, hsp90, and hsp70, although CRT has no apparent structural homologies to them.  (+info)

Peptide-bound major histocompatibility complex class I molecules associate with tapasin before dissociation from transporter associated with antigen processing. (2/690)

Major histocompatibility complex (MHC) class I molecules present antigenic peptides to CD8 T cells. The peptides are generated in the cytosol, then translocated across the membrane of the endoplasmic reticulum by the transporter associated with antigen processing (TAP). TAP is a trimeric complex consisting of TAP1, TAP2, and tapasin (TAP-A) as indicated for human cells by reciprocal coprecipitation with anti-TAP1/2 and anti-tapasin antibodies, respectively. TAP1 and TAP2 are required for the peptide transport. Tapasin is involved in the association of class I with TAP and in the assembly of class I with peptide. The mechanisms of tapasin function are still unknown. Moreover, there has been no evidence for a murine tapasin analogue, which has led to the suggestion that murine MHC class I binds directly to TAP1/2. In this study, we have cloned the mouse analogue of tapasin. The predicted amino acid sequence showed 78% identity to human tapasin with identical consensus sequences of signal peptide, N-linked glycosylation site, transmembrane domain and double lysine motif. However, there was less homology (47%) found at the predicted cytosolic domain, and in addition, mouse tapasin is 14 amino acids longer than the human analogue at the C terminus. This part of the molecule may determine the species specificity for interaction with MHC class I or TAP1/2. Like human tapasin, mouse tapasin binds both to TAP1/2 and MHC class I. In TAP2-mutated RMA-S cells, both TAP1 and MHC class I were coprecipitated by anti-tapasin antiserum indicative of association of tapasin with TAP1 but not TAP2. With crosslinker-modified peptides and purified microsomes, anti-tapasin coprecipitated both peptide-bound MHC class I and TAP1/2. In contrast, anti-calreticulin only coprecipitated peptide-free MHC class I molecules. This difference in association with peptide-loaded class I suggests that tapasin functions later than calreticulin during MHC class I assembly, and controls peptide loading onto MHC class I molecules in the endoplasmic reticulum.  (+info)

Calreticulin is essential for cardiac development. (3/690)

Calreticulin is a ubiquitous Ca2+ binding protein, located in the endoplasmic reticulum lumen, which has been implicated in many diverse functions including: regulation of intracellular Ca2+ homeostasis, chaperone activity, steroid-mediated gene regulation, and cell adhesion. To understand the physiological function of calreticulin we used gene targeting to create a knockout mouse for calreticulin. Mice homozygous for the calreticulin gene disruption developed omphalocele (failure of absorption of the umbilical hernia) and showed a marked decrease in ventricular wall thickness and deep intertrabecular recesses in the ventricular walls. Transgenic mice expressing a green fluorescent protein reporter gene under the control of the calreticulin promoter were used to show that the calreticulin gene is highly activated in the cardiovascular system during the early stages of cardiac development. Calreticulin protein is also highly expressed in the developing heart, but it is only a minor component of the mature heart. Bradykinin-induced Ca2+ release by the InsP3-dependent pathway was inhibited in crt-/- cells, suggesting that calreticulin plays a role in Ca2+ homeostasis. Calreticulin-deficient cells also exhibited impaired nuclear import of nuclear factor of activated T cell (NF-AT3) transcription factor indicating that calreticulin plays a role in cardiac development as a component of the Ca2+/calcineurin/NF-AT/GATA-4 transcription pathway.  (+info)

Antibody levels to recombinant tick calreticulin increase in humans after exposure to Ixodes scapularis (Say) and are correlated with tick engorgement indices. (4/690)

The antibody responses of subjects who presented with a definite Ixodes scapularis (Say) tick bite were measured to determine the utility of the antibody response against recombinant tick calreticulin (rTC) as a biologic marker of tick exposure. Subjects bitten by I. scapularis evidenced an increase in anti-rTC antibody levels between visit 1 and visit 2 from 24.3 to 27.1 ng/microl serum (n = 88, p = 0.003), and levels remained elevated at visit 3 (p = 0.005). These anti-rTC antibody levels during visits 2 and 3 were significantly higher than those in four non-exposed controls. Tick engorgement indices, measured on the biting ticks, were found to be correlated with anti-rTC antibody levels (e.g., for visit 3: Pearson's r = 0.357, p = 0.001). Tick engorgement index (TEI), ratio of body length to scutal width, was identified to be the only independent predictor of anti-rTC antibody levels in linear regression models. Logistic regression revealed that a bite from an I. scapularis tick that became engorged (TEI >3.4) was a risk factor for anti-rTC antibody seropositivity (adjusted odds ratio for age and bite location = 7.4 (95% confidence interval 2.1-26.4)). The anti-rTC antibody test had a sensitivity of 0.50 and a specificity of 0.86 for a bite from I. scapularis that became engorged. Immunoblotting revealed that subjects made a specific anti-rTC antibody response.  (+info)

Calreticulin expression is associated with androgen regulation of the sensitivity to calcium ionophore-induced apoptosis in LNCaP prostate cancer cells. (5/690)

Calreticulin has been identified previously as one of the androgen-response genes in the prostate. The role of calreticulin in androgen action was studied using androgen-sensitive LNCaP and androgen-insensitive PC-3 human prostate cancer cell lines. Calreticulin appears to be a primary androgen-response gene in cultured LNCaP cells because androgen induction of calreticulin mRNA resists protein synthesis inhibition. Calreticulin is a high capacity intracellular Ca2+ binding protein, suggesting that calreticulin expression is likely to be associated with the intracellular Ca2+ buffering capacity that could regulate the sensitivity to cytotoxic intracellular Ca2+ overload. As expected, androgen protects androgen-sensitive LNCaP but not androgen-insensitive PC-3 cells from cytotoxic intracellular Ca2+ overload induced by Ca2+ ionophore A23187. To provide evidence for the role of calreticulin in reducing cytotoxic effect of Ca2+ influx in prostatic cells, we have shown that calreticulin antisense oligonucleotide down-regulates calreticulin protein level and significantly increases the sensitivity to A23187-induced apoptosis in both LNCaP and PC-3 cells. Furthermore, calreticulin antisense oligonucleotide reverses the androgen-induced resistance to A23187 in LNCaP cells. The above observations collectively suggest that calreticulin mediates androgen regulation of the sensitivity to Ca2+ ionophore-induced apoptosis in LNCaP cells.  (+info)

Ligand-specific, transient interaction between integrins and calreticulin during cell adhesion to extracellular matrix proteins is dependent upon phosphorylation/dephosphorylation events. (6/690)

As transmembrane heterodimers, integrins bind to both extracellular ligands and intracellular proteins. We are currently investigating the interaction between integrins and the intracellular protein calreticulin. A prostatic carcinoma cell line (PC-3) was used to demonstrate that calreticulin can be found in the alpha3 immunoprecipitates of cells plated on collagen type IV, but not when plated on vitronectin. Conversely, alphav immunoprecipitates contained calreticulin only when cells were plated on vitronectin, i. e. not when plated on collagen IV. The interactions between these integrins and calreticulin were independent of actin cytoskeleton assembly and were transient, being maximal approx. 10-30 min after the cells came into contact with the substrates prior to complete cell spreading and formation of firm adhesive contacts. We demonstrate that okadaic acid, an inhibitor of intracellular serine/threonine protein phosphatases, inhibited the alpha3beta1-mediated adhesion of PC-3 cells to collagen IV and the alpha2beta1-mediated attachment of Jurkat cells to collagen I. This inhibition by okadaic acid was accompanied by inhibition of the ligand-specific interaction of calreticulin with the respective integrins in the two cell types. Additionally, we found that pharmacological inhibition of mitogen-activated protein kinase kinase (MEK) resulted in prolongation of the calreticulin-integrin interaction, and enhancement of PC-3 cell attachment to collagen IV. We conclude that calreticulin interacts transiently with integrins during cell attachment and spreading. This interaction depends on receptor occupation, is ligand-specific, and can be modulated by protein phosphatase and MEK activity.  (+info)

Trypanosoma cruzi calreticulin is a lectin that binds monoglucosylated oligosaccharides but not protein moieties of glycoproteins. (7/690)

Trypanosoma cruzi is a protozoan parasite that belongs to an early branch in evolution. Although it lacks several features of the pathway of protein N-glycosylation and oligosaccharide processing present in the endoplasmic reticulum of higher eukaryotes, it displays UDP-Glc:glycoprotein glucosyltransferase and glucosidase II activities. It is herewith reported that this protozoan also expresses a calreticulin-like molecule, the third component of the quality control of glycoprotein folding. No calnexin-encoding gene was detected. Recombinant T. cruzi calreticulin specifically recognized free monoglucosylated high-mannose-type oligosaccharides. Addition of anti-calreticulin serum to extracts obtained from cells pulse-chased with [35S]Met plus [35S]Cys immunoprecipitated two proteins that were identified as calreticulin and the lysosomal proteinase cruzipain (a major soluble glycoprotein). The latter but not the former protein disappeared from immunoprecipitates upon chasing cells. Contrary to what happens in mammalian cells, addition of the glucosidase II inhibitor 1-deoxynojirimycin promoted calreticulin-cruzipain interaction. This result is consistent with the known pathway of protein N-glycosylation and oligosaccharide processing occurring in T. cruzi. A treatment of the calreticulin-cruzipain complexes with endo-beta-N-acetylglucosaminidase H either before or after addition of anti-calreticulin serum completely disrupted calreticulin-cruzipain interaction. In addition, mature monoglucosylated but not unglucosylated cruzipain isolated from lysosomes was found to interact with recombinant calreticulin. It was concluded that the quality control of glycoprotein folding appeared early in evolution, and that T. cruzi calreticulin binds monoglucosylated oligosaccharides but not the protein moiety of cruzipain. Furthermore, evidence is presented indicating that glucosyltransferase glucosylated cruzipain at its last folding stages.  (+info)

Calreticulin affects focal contact-dependent but not close contact-dependent cell-substratum adhesion. (8/690)

We used two cell lines expressing fast (RPEfast) and slow (RPEslow) attachment kinetics to investigate mechanisms of cell-substratum adhesion. We show that the abundance of a cytoskeletal protein, vinculin, is dramatically decreased in RPEfast cells. This coincides with the diminished expression level of an endoplasmic reticulum chaperone, calreticulin. Both protein and mRNA levels for calreticulin and vinculin were decreased in RPEfast cells. After RPEfast cells were transfected with cDNA encoding calreticulin, both the expression of endoplasmic reticulum-resident calreticulin and cytoplasmic vinculin increased. The abundance of other adhesion-related proteins was not affected. RPEfast cells underexpressing calreticulin displayed a dramatic increase in the abundance of total cellular phosphotyrosine suggesting that the effects of calreticulin on cell adhesiveness may involve modulation of the activities of protein tyrosine kinases or phosphatases which may affect the stability of focal contacts. The calreticulin and vinculin underexpressing RPEfast cells lacked extensive focal contacts and adhered weakly but attached fast to the substratum. In contrast, the RPEslow cells that expressed calreticulin and vinculin abundantly developed numerous and prominent focal contacts slowly, but adhered strongly. Thus, while the calreticulin overexpressing RPEslow cells "grip" the substratum with focal contacts, calreticulin underexpressing RPEfast cells use close contacts to "stick" to it.  (+info)

Regulation of calreticulin gene expression by calcium<...
TY - JOUR. T1 - Regulation of calreticulin gene expression by calcium. AU - Waser, Mathilde. AU - Mesaeli, Nasrin. AU - Spencer, Charlotte. AU - Michalak, Marek. PY - 1997/8/11. Y1 - 1997/8/11. N2 - We have isolated and characterized a 12-kb mouse genomic DNA fragment containing the entire calreticulin gene and 2.14 kb of the promoter region. The mouse calreticulin gene consists of nine exons and eight introns, and it spans 4.2 kb of genomic DNA. A 1.8-kb fragment of the calreticulin promoter was subcloned into a reporter gene plasmid containing chloramphenicol acetyltransferase. This construct was then used in transient and stable transfection of NIH/3T3 cells. Treatment of transfected cells either with the Ca2+ ionophore A23187, or with the ER Ca2+-ATPase inhibitor thapsigargin, resulted in a five- to sevenfold increase of the expression of chloramphenicol acetyltransferase protein. Transactivation of the calreticulin promoter was also increased by fourfold in NIH/3T3 cells treated with ...
https://qfrd.pure.elsevier.com/en/publications/regulation-of-calreticulin-gene-expression-by-calcium
Recombinant Human Calreticulin protein (ab40609) | AbcamRecombinant Human Calreticulin protein (ab40609) | Abcam
Buy our Recombinant Human Calreticulin protein. Ab40609 is a protein fragment produced in Escherichia coli and has been validated in SDS-PAGE, MS. Abcam…
http://www.abcam.com/recombinant-human-calreticulin-protein-ab40609.html
Anti-Calreticulin Antibody | 06-661Anti-Calreticulin Antibody | 06-661
Anti-Calreticulin Antibody is an antibody against Calreticulin for use in IC, IH & WB. Find MSDS or SDS, a COA, data sheets and more information.
http://www.merckmillipore.com/INTL/en/product/Anti-Calreticulin-Antibody,MM_NF-06-661
Toxins | Free Full-Text | SS1P Immunotoxin Induces Markers of Immunogenic Cell Death and Enhances the Effect of the CTLA-4...Toxins | Free Full-Text | SS1P Immunotoxin Induces Markers of Immunogenic Cell Death and Enhances the Effect of the CTLA-4...
SS1P is an anti-mesothelin immunotoxin composed of a targeting antibody fragment genetically fused to a truncated fragment of Pseudomonas exotoxin A. Delayed responses reported in mesothelioma patients receiving SS1P suggest that anti-tumor immunity is induced. The goal of this study is to evaluate if SS1P therapy renders mesothelioma tumors more sensitive to cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) immune checkpoint blockade. We evaluated the ability of SS1P to induce adenosine triphosphate (ATP) secretion and calreticulin expression on the surface of AE17M mouse mesothelioma cells. Both properties are associated with immunogenic cell death. Furthermore, we treated these tumors with intra-tumoral SS1P and systemic CTLA-4. We found that SS1P increased the release of ATP from AE17M cells in a dose and time-dependent manner. In addition, SS1P induced calreticulin expression on the surface of AE17M cells. These results suggest that SS1P promotes immunogenic cell death and could sensitize tumors
https://www.mdpi.com/2072-6651/10/11/470
Functional Analysis of Recombinant Calreticulin Fragment 39-272: Implications for Immunobiological Activities of Calreticulin...Functional Analysis of Recombinant Calreticulin Fragment 39-272: Implications for Immunobiological Activities of Calreticulin...
Although calreticulin (CRT) is a major Ca2+-binding luminal resident protein, it can also appear on the surface of various types of cells and it functions as an immunopotentiating molecule. However, molecular mechanisms underlying the potent immunobiological activity of cell surface CRT are still unclear. In the present study, a recombinant fragment (rCRT/39-272) covering the lectin-like N domain and partial P domain of murine CRT has been expressed in Escherichia coli. The affinity-purified rCRT/39-272 assembles into homodimers and oligomers in solution and exhibits high binding affinity to various glycans, including carrageenan, alginic acids, and hyaluronic acids. Functionally, rCRT/39-272 is capable of driving the activation and maturation of B cells and cytokine production by macrophages in a TLR-4-dependent manner in vitro. It specifically binds recombinant mouse CD14, but not BAFFR and CD40. It is also able to trigger Ig class switching by B cells in the absence of T cell help both in ...
http://www.jimmunol.org/content/early/2010/09/20/jimmunol.1000536
Vasostatin, a Calreticulin Fragment, Inhibits Angiogenesis and Suppresses Tumor Growth | JEMVasostatin, a Calreticulin Fragment, Inhibits Angiogenesis and Suppresses Tumor Growth | JEM
These results show that vasostatin, an NH2-terminal fragment of human calreticulin, can inhibit endothelial cell proliferation in vitro, suppress neovascularization in vivo, and prevent or reduce growth of experimental tumors. Calreticulin, a ubiquitous and highly conserved protein originally identified in skeletal muscle sarcoplasmic reticulum, serves as one of the major storage depots for calcium ions within the endoplasmic reticulum and participates in calcium signaling ((34)-(36)). The NH2-domain of calreticulin, which includes aa 1-180, is the most conserved domain among the calreticulins so far cloned and has no homology to other protein sequences ((34), (35)). Although it does not bind calcium, it can bind the cytoplasmic domain of α subunits of integrins regulating cell attachment ((37)), can interact with the nuclear receptors for glucocorticoid, androgen, and retinoic acid, regulating their binding to DNA ((38)), and can, once phosphorylated, bind stem-loop structures at the 3′-end ...
http://jem.rupress.org/content/188/12/2349
anti-Calreticulin antibody | GeneTexanti-Calreticulin antibody | GeneTex
Calreticulin antibody (calreticulin) for ICC/IF, IHC-P, WB. Anti-Calreticulin pAb (GTX111627) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
http://www.genetex.com/Calreticulin-antibody-GTX111627.html
Enhanced EGFR expression and function in calreticulin deficient cells | QScience.comEnhanced EGFR expression and function in calreticulin deficient cells | QScience.com
Abstract Introduction: Calreticulin is a multi-functioning protein located in the endoplasmic reticulum. Several functions have been attributed to calreticulin including lectin-like chaperoning, regulation of gene expression, cell adhesion, auto-immunity and calcium homeostasis. As an endoplasmic reticulum chaperone calreticulin regulates the maturation and folding of several trans-membrane proteins. We hypothesized that as an endoplasmic reticular protein it regulates the expression folding and maturation of epidermal growth factor receptor (EGFR). To date no information is available about the role of calreticulin in EGFR expression and folding. Methods: Wild type calreticulin deficient (crt -/-) and mouse lung cancer cells isolated from transgenic mice over expressing calreticulin was used to examine the expression, localization and function of EGFR. Western blot analysis was used to determine the protein expression. Immunocytochemical staining of cells was utilized to determine localization of EGFR
https://www.qscience.com/content/papers/10.5339/qfarf.2010.BMPS4
Ca2+ release in saponin-permeabilized calreticulin-defi | Open-iCa2+ release in saponin-permeabilized calreticulin-defi | Open-i
Ca2+ release in saponin-permeabilized calreticulin-deficient cells. Wild-type (K41) and calreticulin-deficient (K42) cells were loaded with a fluorescent Ca2+ i
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2196195_0102073f8&req=4
JCI - WelcomeJCI - Welcome
Blockade of the checkpoint inhibitor programmed death 1 (PD1) has demonstrated remarkable success in the clinic for the treatment of cancer; however, a majority of tumors are resistant to anti-PD1 monotherapy. Numerous ongoing clinical combination therapy studies will likely reveal additional therapeutics that complement anti-PD1 blockade. Recent studies found that immunogenic cell death (ICD) improves T cell responses against different tumors, thus indicating that ICD may further augment antitumor immunity elicited by anti-PD1. Here, we observed antitumor activity following combinatorial therapy with anti-PD1 Ab and the cyclin-dependent kinase inhibitor dinaciclib in immunocompetent mouse tumor models. Dinaciclib induced a type I IFN gene signature within the tumor, leading us to hypothesize that dinaciclib potentiates the effects of anti-PD1 by eliciting ICD. Indeed, tumor cells treated with dinaciclib showed the hallmarks of ICD including surface calreticulin expression and release of high ...
https://5co1nn1.cn.insight.mobile.jci.org/tags/33
Dokument bez názvuDokument bez názvu
Quality control of the endoplasmic reticulum plays a critical role in protein folding, modification and modification of a secretory pathway. As endoplasmic reticulum chaperones, calreticulin and calnexin have similar substrate specificity and share several common features. Yet, surprisingly, mice bearing a disruption in the calreticulin gene die from a lesion in cardiac development and develop significant metabolic problems whereas calnexin-deficient mice are born alive with, yet not understood, neurological problems. Studies with calreticulin and calnexin gene knockout mice and calreticulin- and calnexindeficient cell lines indicate that calnexin is unable to compensate for the loss of calreticulin and conversely, calreticulin cannot compensate for the loss of calnexin. Calreticulin or calnexin deficiency or reduction in the level of ERp57 protein (ERp57 heterozygote mice) leads to development of metabolic disorders as documented by sever changes serum lipids and carbohydrates composition in ...
http://jab.zsf.jcu.cz/3_4/michalak.htm
Modulation of gene expression by calreticulin binding to the glucocorticoid receptorModulation of gene expression by calreticulin binding to the glucocorticoid receptor
Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin has been reported to bind to the synthetic peptide …
https://pubmed.ncbi.nlm.nih.gov/8107808/?dopt=Abstract
2021 CRT-211 New Question - CRT-211 Certification Exam, Salesforce Certified Advanced Administrator Exam Latest Test...2021 CRT-211 New Question - CRT-211 Certification Exam, Salesforce Certified Advanced Administrator Exam Latest Test...
Efficient CRT-211 New Question - Easy and Guaranteed CRT-211 Exam Success, We can ensure you that you will receive our CRT-211 practice exam materials within 5 to 10 minutes after payment, this marks the fastest delivery speed in this field, Salesforce CRT-211 New Question Chances favor the prepared mind, Passing a CRT-211 exam to get a certificate will help you to look for a better job and get a higher salary, If you are the first time to prepare the CRT-211 exam, it is better to choose a type of good study materials.
http://www.piece-banana.com/braindumps-New-Question-404051/CRT-211-exam.html
In vitro Assays for the Detection of Calreticulin Exposure, ATP and HMGB1 Release upon Cell Death -BIO-PROTOCOLIn vitro Assays for the Detection of Calreticulin Exposure, ATP and HMGB1 Release upon Cell Death -BIO-PROTOCOL
Accumulating evidence is revealing the essential role of immune system in cancer treatment. Certain chemotherapeutic drugs can potently induce the release of cell death associated molecular patterns (CDAMPs), which accompanies cancer cell demise. CDAMPs can engage corresponding receptors on immune cells and stimulate immune responses to achieve long-term tumor control (Ma et al., 2013; Ma et al., 2014; Yang et al., 2015). Among reported CDAMPs, calreticulin (CALR), ATP and HMGB1 are well known for their immune-stimulatory effect. Here we describe the assays that we applied to measure cell death and these CDAMPs. Briefly, cell death can be analyzed by co-staining of 4,6-diamidino-2-phenylindole (DAPI) with 3,3-Dihexyloxacarbocyanine Iodide [DiOC6(3)] or Annexin V. CALR exposure on the cell membrane can be detected by flow cytometry. ATP and HMGB1 release can be quantified by luminescence assay and ELISA assay respectively.
https://bio-protocol.org/p.aspx?id=e2076
Evolving Evidence of Calreticulin as a Pharmacological Target in Neurological Disorders<...
TY - JOUR. T1 - Evolving Evidence of Calreticulin as a Pharmacological Target in Neurological Disorders. AU - Kotian, Vignesh. AU - Sarmah, Deepaneeta. AU - Kaur, Harpreet. AU - Kesharwani, Radhika. AU - Verma, Geetesh. AU - Mounica, Leela. AU - Veeresh, Pabbala. AU - Kalia, Kiran. AU - Borah, Anupom. AU - Wang, Xin. AU - Dave, Kunjan R. AU - Yavagal, Dileep R. AU - Bhattacharya, Pallab. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Calreticulin (CALR), a lectin-like ER chaperone, was initially known only for its housekeeping function, but today it is recognized for many versatile roles in different compartments of a cell. Apart from canonical roles in protein folding and calcium homeostasis, it performs a variety of noncanonical roles, mostly in CNS development. In the past, studies have linked Calreticulin with various other biological components which are detrimental in deciding the fate of neurons. Many neurological disorders that differ in their etiology are commonly associated with aberrant levels of ...
https://miami.pure.elsevier.com/en/publications/evolving-evidence-of-calreticulin-as-a-pharmacological-target-in-
Regulation of the Calreticulin Gene by GATA6 and Evi-1 Transcription Factors †Regulation of the Calreticulin Gene by GATA6 and Evi-1 Transcription Factors †
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https://www.infona.pl/resource/bwmeta1.element.acs-doi-10_1021_bi702524v
RCSB PDB - 5LK5: Crystal structure of the globular domain of human calreticulin mutant D71KRCSB PDB - 5LK5: Crystal structure of the globular domain of human calreticulin mutant D71K
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
https://www.rcsb.org/structure/5LK5
Calreticulin - CFGparadigmsCalreticulin - CFGparadigms
This family of GBPs is widespread in evolution and plays a key role in ER quality control,ref name=Ellgaard 2003a,Ellgaard, L. and Frickel, E. M. Calnexin, calreticulin, and ERp57: teammates in glycoprotein folding. Cell Biochem Biophys 39, 223-247 (2003),/ref,,ref name=Jorgensen 2003,Jorgensen, M. M., Bross, P. and Gregersen, N. Protein quality control in the endoplasmic reticulum. APMIS Suppl 86-91 (2003),/ref,,ref name=Ellgaard 2003,Ellgaard, L. and Helenius, A. Quality control in the endoplasmic reticulum. Nat Rev Mol Cell Biol 4, 181-191 (2003),/ref,,ref name=Helenius 2004,Helenius, A. and Aebi, M. Roles of N-linked glycans in the endoplasmic reticulum. Annu Rev Biochem 73, 1019-1049 (2004),/ref,,ref,Molinari, M., Eriksson, K. K., Calanca, V., Galli, C., Cresswell, P., Michalak, M. and Helenius, A. Contrasting functions of calreticulin and calnexin in glycoprotein folding and ER quality control. Mol Cell 13, 125-135 (2004),/ref,,ref,Deprez, P., Gautschi, M. and Helenius, A. More ...
http://functionalglycomics.org/CFGparadigms/index.php?title=Calreticulin&diff=prev&oldid=1236
Antibody to calreticulinAntibody to calreticulin
Does anyone have an antibody to human calreticulin that they can let us have for work on rheumatoid arthritis? Thanks Frank -- Frank C Hay Division of Immunology St Georges Hospital Medical School London SW17 0RE, UK Tel: 0181 767 5751 Fax: 0181 725 3549 email: frank at rabbits.demon.co.uk ...
http://www.bio.net/bionet/mm/immuno/1995-May/003939.html
Sourcing the immune system to induce immunogenic cell death in Kras-colorectal cancer cells | British Journal of CancerSourcing the immune system to induce immunogenic cell death in Kras-colorectal cancer cells | British Journal of Cancer
Current approaches aimed at inducing immunogenic cell death (ICD) to incite an immune response against cancer neoantigens are based on the use of chemotherapeutics and other agents. Results are hampered by issues of efficacy, combinatorial approaches, dosing and toxicity. Here, we adopted a strategy based on the use of an immunomolecule that overcomes pharmachemical limitations. Cytofluorometry, electron microscopy, RT-PCR, western blotting, apotome immunofluorescence, MLR and xenografts. We report that an ICD process can be activated without the use of pharmacological compounds. We show that in Kras-mut/TP53-mut colorectal cancer cells the 15 kDa βGBP cytokine, a T cell effector with onco-suppressor properties and a potential role in cancer immunosurveillance, induces key canonical events required for ICD induction. We document ER stress, autophagy that extends from cancer cells to the corresponding xenograft tumours, CRT cell surface shifting, ATP release and evidence of dendritic cell activation, a
https://www.nature.com/articles/s41416-019-0561-z?error=cookies_not_supported&code=4ae5702e-a06c-4393-81e1-faa684e993e1
Molecular and Translational Classifications of DAMPs in Immunogenic Cell Death. - Immunology
The immunogenicity of malignant cells has recently been acknowledged as a critical determinant of efficacy in cancer therapy. Thus, besides developing direct immunostimulatory regimens, including dendritic cell-based vaccines, checkpoint-blocking therapies, and adoptive T-cell transfer, researchers have started to focus on the overall immunobiology of neoplastic cells. It is now clear that cancer cells can succumb to some anticancer therapies by undergoing a peculiar form of cell death that is characterized by an increased immunogenic potential, owing to the emission of the so-called damage-associated molecular patterns (DAMPs). The emission of DAMPs and other immunostimulatory factors by cells succumbing to immunogenic cell death (ICD) favors the establishment of a productive interface with the immune system. This results in the elicitation of tumor-targeting immune responses associated with the elimination of residual, treatment-resistant cancer cells, as well as with the establishment of
https://www.immunology.ox.ac.uk/publications/589318
Crizotinib-induced immunogenic cell death in non-small cell lung cancer - HTE Program
Immunogenic cell death (ICD) converts dying cancer cells into a therapeutic vaccine and stimulates antitumor immune responses. Here we unravel the results of an unbiased screen identifying high-dose (10 µM) crizotinib as an ICD-inducing tyrosine kinase inhibitor that has exceptional antineoplastic activity when combined with non-ICD inducing chemotherapeutics like cisplatin. The combination of cisplatin and high-dose crizotinib induces ICD in non-small cell lung carcinoma (NSCLC) cells and effectively controls the growth of distinct (transplantable, carcinogen- or oncogene induced) orthotopic NSCLC models. These anticancer effects are linked to increased T lymphocyte infiltration and are abolished by T cell depletion or interferon-γ neutralization. Crizotinib plus cisplatin leads to an increase in the expression of PD-1 and PD-L1 in tumors, coupled to a strong sensitization of NSCLC to immunotherapy with PD-1 antibodies. Hence, a sequential combination treatment consisting in conventional ...
http://hteprogram.com/crizotinib-induced-immunogenic-cell-death-in-non-small-cell-lung-cancer/
Calreticulin Summary Report | CureHunterCalreticulin Summary Report | CureHunter
Calreticulin: A multifunctional protein that is found primarily within membrane-bound organelles. In the ENDOPLASMIC RETICULUM it binds to specific N-linked oligosaccharides found on newly-synthesized proteins and functions as a MOLECULAR CHAPERONE that may play a role in PROTEIN FOLDING or retention and degradation of misfolded proteins. In addition calreticulin is a major storage form for CALCIUM and functions as a calcium-signaling molecule that can regulate intracellular calcium HOMEOSTASIS.
http://www.curehunter.com/public/keywordSummaryD037282-Calreticulin.do
Zinc-Dependent Homomultimerization of Mutant Calreticulin Is Required for MPN Pathogenesis | Blood | American Society of...Zinc-Dependent Homomultimerization of Mutant Calreticulin Is Required for MPN Pathogenesis | Blood | American Society of...
Recurrent mutations in the ER chaperone calreticulin (CALR) are found in ~30% of MPNs. All known CALR mutations result in a +1-frameshift of the CALR reading fr
https://ashpublications.org/blood/article-split/134/Supplement_1/312/426012/Zinc-Dependent-Homomultimerization-of-Mutant
Spreading of the immune response from 52 kDaRo and 60 kDaRo to calreticulin in experimental autoimmunity<...
TY - JOUR. T1 - Spreading of the immune response from 52 kDaRo and 60 kDaRo to calreticulin in experimental autoimmunity. AU - Kinoshita, G.. AU - Keech, C. L.. AU - Sontheimer, R. D.. AU - Purcell, A.. AU - McCluskey, J.. AU - Gordon, T. P.. PY - 1998/2/9. Y1 - 1998/2/9. N2 - Calreticulin (CR) is widely recognized as a new human autoantigen but there are conflicting data concerning its relationship with the Ro(SS-A) ribonucleoprotein (RNP). Recent evidence suggests that CR binds to 52 kDaRo (Ro52) by a protein/protein interaction and binds to hY RNA and rubella virus RNA. Other studies have shown that initiation of immunity to either Ro52 or 60 kDaRo (Ro60) can lead to reciprocal spreading of autoimmunity to Ro60 or Ro52, respectively, and induce anti-La autoantibodies in some strains of mice. These findings support a physical association of these polypeptides in Ro/La complexes. To test the hypothesis that CR is physically associated with Ro52 and/or Ro60 we examined the sera of Ro52-, Ro60- ...
https://researchnow.flinders.edu.au/en/publications/spreading-of-the-immune-response-from-52-kdaro-and-60-kdaro-to-ca
Most Popular CRT-251 Testing Technology - Free Salesforce CRT-251 Test Braindump - CRT-251 Exam Materials Online at Actual...Most Popular CRT-251 Testing Technology - Free Salesforce CRT-251 Test Braindump - CRT-251 Exam Materials Online at Actual...
Actual Exams Sales Cloud Consultant training service - Salesforce CRT-251 online test materials with real CRT-251 practice exam questions.
https://www.actual-exams.com/CRT-251-practice-exam.htm
Immunogenic tumor cell death induced by chemotherapy in patients with breast cancer and esophageal squamous cell carcinomaImmunogenic tumor cell death induced by chemotherapy in patients with breast cancer and esophageal squamous cell carcinoma
The present study contains novel findings supporting the concept that ICD can be induced by chemotherapy alone in patients with breast cancer and ESCC. Firstly, both HMGB1 and calreticulin expression were significantly upregulated after NAC. Secondly, chemotherapeutic drugs induced the upregulation of HMGB1 and calreticulin in several tested breast cancer cell lines.. We and others have recently reported that danger signals from dying cells following treatment with radiotherapy or certain chemotherapeutic drugs, such as anthracyclines and oxaliplatin, can induce Toll-like receptor (TLR)-dependent, antigen-specific T-cell immunity (22,23). Additional therapeutic modalities shown to induce ICD include oncolytic virus therapy (24-26) and photodynamic therapy (27,28). Furthermore, among various danger signals released from dying cells in a tumor-bearing mouse model, HMGB1, but not other known TLR4 ligands, could be a mandatory factor to induce tumor antigen-specific T-cell immunity (22,23). ...
https://spandidos-publications.com/10.3892/or.2017.6097
Plus itPlus it
The present study contains novel findings to support the concept of immunogenic cell death induced by chemoradiotherapy in patients with ESCC. First, tumor antigen-specific T-cell responses were confirmed in 6 (38%) of 16 patients with ESCC following chemoradiotherapy. Second, the serum level of HMGB1 following chemoradiation in the patients with antigen-specific T-cell responses was significantly elevated in comparison to that in the patients without antigen-specific T-cell responses. Third, upregulation of HMGB1 within tumor microenvironments was significantly related to preoperative chemoradiotherapy and the degree of HMGB1 positively correlated with patients survival. Fourth, both irradiation and chemodrugs could induce upregulation of HMGB1 and calreticulin on ESCC cell lines in vitro. Finally, HMGB1 was able to induce maturation of DCs in an in vitro culture system.. In general, chemoradiotherapy is thought to induce an immunosuppressive state in both T-cell and natural killer-cell ...
http://cancerres.aacrjournals.org/content/72/16/3967
CALRETICULIN workshopCALRETICULIN workshop
Dear All Please read attached document for details of 4th international CALRETICULIN workshop to be held at Oxford University in April 2000 If you have problems opening the attachment or are worried about viruses look at our homepage on:- http://www.uwcm.ac.uk/uwcm/mb/crt2000.html Yours sincerely, Paul Eggleton. Dept Biochemistry. University of Oxford. UK ...
http://www.bio.net/bionet/mm/arab-gen/1999-June/007081.html
Researchers show how mutant CALR protein triggers cancerResearchers show how mutant CALR protein triggers cancer
Researchers at Juntendo University report in the journal Leukemia how mutants of the protein calreticulin lead to molecular mechanisms triggering myeloproliferative neoplasms, which can cause cancer. The findings may lead to the development of novel therapies for certain types of blood cancer.
https://www.news-medical.net/news/20180628/Researchers-show-how-mutant-CALR-protein-triggers-cancer.aspx
Plus itPlus it
Two key factors that mediate high-glucose-induced downregulation of the glucose transport system in bovine aortic endothelial cells and in the human-derived EA.hy926 cells have been identified: the lipid peroxidation product 4-HDDE and its cognate nuclear receptor PPARδ. The latter increases the expression of the protein calreticulin that was shown before to destabilize GLUT-1 mRNA (13).. The augmented production of 4-HDDE results from high-glucose-induced 12-LO expression and activity and glucose-derived ROS. The mechanism responsible for the increased expression of 12-LO is not yet known. Numerous studies have proven that hyperglycemia promotes the generation of ROS (3,7). We showed before an augmented production of ROS in bovine aortic endothelial cell primary cultures under high-glucose conditions (28). Two observations confirm the role of ROS in the generation of 4-HDDE from 12-LO metabolites. First, the inhibition of 12-LO activity with baicalein significantly reduced 4-HDDE secretion ...
http://diabetes.diabetesjournals.org/content/59/4/808
Trial watch: chemotherapy-induced immunogenic cell death in immuno-oncology. | SotioTrial watch: chemotherapy-induced immunogenic cell death in immuno-oncology. | Sotio
The term immunogenic cell death (ICD) denotes an immunologically unique type of regulated cell death that enables, rather than suppresses, T cell-driven immune responses that are specific for antigens derived from the dying cells. The ability of ICD to elicit adaptive immunity heavily relies on the immunogenicity of dying cells, implying that such cells must encode and present antigens not covered by central tolerance (antigenicity), and deliver immunostimulatory molecules such as damage-associated molecular patterns and cytokines (adjuvanticity). Moreover, the host immune system must be equipped to detect the antigenicity and adjuvanticity of dying cells. As cancer (but not normal) cells express several antigens not covered by central tolerance, they can be driven into ICD by some therapeutic agents, including (but not limited to) chemotherapeutics of the anthracycline family, oxaliplatin and bortezomib, as well as radiation therapy. In this Trial Watch, we describe current trends in the ...
https://www.sotio.com/news-publications/list-of-publications/trial-watch-chemotherapy-induced-immunogenic-cell-death-in-immuno-oncology
Oxygen and oxaliplatin-loaded nanoparticles combined with photo-sonodynamic inducing enhanced immunogenic cell death in...Oxygen and oxaliplatin-loaded nanoparticles combined with photo-sonodynamic inducing enhanced immunogenic cell death in...
Immunotherapy by stimulating the host immune system has been a promising therapeutic strategy for advanced ovarian cancer. Here we describe a treatment strategy that combines chemotherapy and photo-sonodynamic therapy (PSDT) to induce systemic antitumor immunity. We have successfully fabricated phase-changeable core-shell nanoparticles (OIX_NPs), which carry oxygen in the core and the photosensitizer indocyanine green (ICG)/oxaliplatin (OXP) in the shell for our combination therapy.
https://www.visualsonics.com/publication/oxygen-and-oxaliplatin-loaded-nanoparticles-combined-photo-sonodynamic-inducing-enhanced
Dendritic cell vaccines based on immunogenic cell death elicit danger signals and T cell-driven rejection of high-grade glioma ...Dendritic cell vaccines based on immunogenic cell death elicit danger signals and T cell-driven rejection of high-grade glioma ...
Thank you for your interest in spreading the word about Science Translational Medicine.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
https://stm.sciencemag.org/content/8/328/328ra27/tab-pdf
Immunogenic cell death in cancer therapy. - PubMed - NCBIImmunogenic cell death in cancer therapy. - PubMed - NCBI
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
https://www.ncbi.nlm.nih.gov/pubmed/23157435
Differential association of calreticulin type 1 and type 2 mutations with myelofibrosis and essential thrombocytemia: relevance...Differential association of calreticulin type 1 and type 2 mutations with myelofibrosis and essential thrombocytemia: relevance...
Nangalia J., Massie C.E., Baxter E.J., Nice F.L., Gundem G., Wedge D.C., Avezov E., Li J., Kollmann K., Kent D.G., Aziz A., Godfrey A.L., Hinton J., Martincorena I., Van Loo P., Jones A.V., Guglielmelli P., Tarpey P., Harding H.P., Fitzpatrick J.D., Goudie C.T., Ortmann C.A., Loughran S.J., Raine K., Jones D.R., Butler A.P., Teague J.W., OMeara S., McLaren S., Bianchi M., Silber Y., Dimitropoulou D., Bloxham D., Mudie L., Maddison M., Robinson B., Keohane C., Maclean C., Hill K., Orchard K., Tauro S., Du M.-Q., Greaves M., Bowen D., Huntly B.J.P., Harrison C.N., Cross N.C.P., Ron D., Vannucchi A.M., Papaemmanuil E., Campbell P.J., Green A.R., Somatic CALR Mutations in Myeloproliferative Neoplasms with Nonmutated JAK2, 10.1056/nejmoa1312542 ...
https://dial.uclouvain.be/pr/boreal/object/boreal:155936
Calr - Calreticulin - Mus musculus (Mouse) - Calr gene & proteinCalr - Calreticulin - Mus musculus (Mouse) - Calr gene & protein
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
http://www.uniprot.org/uniprot/B2MWM9
JCI - Usage information: Cyclin-dependent kinase inhibitor 2B regulates efferocytosis and atherosclerosisJCI - Usage information: Cyclin-dependent kinase inhibitor 2B regulates efferocytosis and atherosclerosis
Genetic variation at the chromosome 9p21 risk locus promotes cardiovascular disease; however, it is unclear how or which proteins encoded at this locus contribute to disease. We have previously demonstrated that loss of one candidate gene at this locus, cyclin-dependent kinase inhibitor 2B (Cdkn2b), in mice promotes vascular SMC apoptosis and aneurysm progression. Here, we investigated the role of Cdnk2b in atherogenesis and found that in a mouse model of atherosclerosis, deletion of Cdnk2b promoted advanced development of atherosclerotic plaques composed of large necrotic cores. Furthermore, human carriers of the 9p21 risk allele had reduced expression of CDKN2B in atherosclerotic plaques, which was associated with impaired expression of calreticulin, a ligand required for activation of engulfment receptors on phagocytic cells. As a result of decreased calreticulin, CDKN2B-deficient apoptotic bodies were resistant to efferocytosis and not efficiently cleared by neighboring macrophages. These ...
https://www.jci.org/articles/view/70391/usage
KAKEN - Research Projects | Overexpression of calreticulin modulates protein kinase B/Akt signaling to promote apoptosis during...KAKEN - Research Projects | Overexpression of calreticulin modulates protein kinase B/Akt signaling to promote apoptosis during...
Principal Investigator：TODA Genji, Project Period (FY)：2001 - 2002, Research Category：Grant-in-Aid for Scientific Research (C), Section：一般, Research Field：Circulatory organs internal medicine
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670726/
calreticulin 3 (mouse)calreticulin 3 (mouse)
The lollipop plot above illustrates recurrent (observed in 3 or more out of 4440 TCGA tumor samples from 15 cancer types) and therefore potentially oncogenic missense mutations (click on Show Cancer Mutations). The bar plot below shows the proportion of tumor samples that have any kind of altering mutation(s) in the given protein. ...
https://www.phosphosite.org/proteinAction.action?id=8421&showAllSites=true
Targeted therapy and radiation drug combinations ( - University of Manchester)
Lead: Tim Illidge. Radiation treatment (RT) is a highly effective anti-cancer treatment that is delivered to over 50% of all cancer patients and 40% of those cured of their disease.. In order to further improve cancer outcomes using RT, an increased understanding of what determines effective RT-induced anti-tumour responses and how best to combine RT with others treatments is required.. Our research programme evaluates the contribution of RT-induced immunogenic cell death to the induction of tumour-specific immune responses (Project 1); determines how best to integrate RT with immunomodulatory agents to augment such responses and enhance therapeutic outcome (Project 2); and investigates how combination with RT and immune modulation can be utilised to increase the efficacy and durability of anti-CD20 mAb therapy in B- cell lymphomas (Project 3).. The pre-clinical experimental programme runs in parallel with early phase clinical trials to form a cohesive and overlapping programme of work that aims ...
http://research.bmh.manchester.ac.uk/radiotherapy/research/targetedtherapy
Plus itPlus it
Purpose: To characterize the ability of PT-112 to induce immunogenic cell death (ICD) in vitro and in vivo. Background: PT-112 is a novel chemical entity consisting of a platinum core conjugated to a pyrophosphate and diaminocyclohexane groups. In vitro studies demonstrated that PT-112 has both cytostatic and cytotoxic effects on human cancer cells, two effects that are seen in the absence of robust binding to nuclear DNA. Accordingly, PT-112 potency is largely unaffected by functional DNA repair pathways, suggesting that PT-112 operates with mechanisms that differ from conventional DNA-damaging chemotherapies. PT-112 is currently under phase I/II clinical development in patients with solid tumors and hematologic malignancies, both as monotherapy and in combination with a PD-L1 inhibitor. Interim reports have established encouraging tolerability and signals of single-agent anticancer activity. Previous work with human colorectal cancer HCT-116 cells demonstrated that PT-112 causes the release of ...
https://cancerimmunolres.aacrjournals.org/content/7/2_Supplement/B199
Addgene: mEmerald-Calreticulin-C-10Addgene: mEmerald-Calreticulin-C-10
Plasmid mEmerald-Calreticulin-C-10 from Dr. Michael Davidsons lab contains the insert Calreticulin. This plasmid is available through Addgene.
https://www.addgene.org/54022/
CALR antibody (ARP30114 P050) | Tested with: Human HepG2 cellsCALR antibody (ARP30114 P050) | Tested with: Human HepG2 cells
CALR antibody - C-terminal region (ARP30114_P050) | Application: WB | CALR is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells | Alias: RO; CRT; SSA; cC1qR
https://www.avivasysbio.com/calr-antibody-c-terminal-region-arp30114-p050.html
Gene Variant DetailGene Variant Detail
CALR D45Y lies within the N-domain region of the Calr protein (UniProt.org). D45Y has been identified in the scientific literature (PMID: 29218307) but has not been biochemically characterized and therefore, its effect on Calr protein function is unknown (PubMed, Feb 2020 ...
https://ckb.jax.org/geneVariant/show?geneVariantId=14425
Effects of prenatal glucocorticoid exposure on cardiac calreticulin and calsequestrin protein expression during early...Effects of prenatal glucocorticoid exposure on cardiac calreticulin and calsequestrin protein expression during early...
Overexpression of the conserved Ca2+-binding proteins calreticulin and calsequestrin impairs cardiac function, leading to premature death. Calreticulin is vital for embryonic development, but also impairs glucocorticoid action. Glucocorticoid overexposure during late fetal life causes intra-uterine growth retardation and programmed hypertension in adulthood. To determine whether intra-uterine growth retardation or programmed hypertension was associated with altered calreticulin or calsequestrin expression, effects of prenatal glucocorticoid overexposure (maternal dexamethasone treatment on days 15-21 of pregnancy) were examined during fetal life and postnatal development until adulthood (24 weeks). Dexamethasone (100 or 200μg/kg of maternal body weight) was administered via osmotic pump. Calreticulin was detected as a 55kDa band and calsequestrin as 55 and 63kDa bands in 21 day fetal hearts. Only the 55kDa calsequestrin band was detected postnatally. Prenatal glucocorticoid overexposure at the ...
http://www.biochemj.org/content/371/1/61
Synthetic immunogenic cell death mediated by intracellular delivery of STING agonist nanoshells enhances anti-cancer chemo...Synthetic immunogenic cell death mediated by intracellular delivery of STING agonist nanoshells enhances anti-cancer chemo...
Many favorable anti-cancer treatments owe their success to the induction immunogenic cell death (ICD) in cancer cells, which activates antigen presenting cells to prime anti-cancer adaptive immunity. We describe a strategy to synthetically induce ICD by delivering the agonist of stimulator of interferon genes (STING), 23′-cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) into tumor cells using hollow polymeric nanoshells. Following intracellular delivery of exogenous adjuvant, subsequent cytotoxic treatment creates immunogenic cellular debris, by a process herein termed synthetic immunogenic cell death (sICD). sICD is indiscriminate to the type of chemotherapeutics adopted for cancer treatment and enables co-localization of exogenously administered immunologic adjuvants and tumor antigens for enhanced antigen presentation and development of anticancer adaptive immunity. In three mouse tumor models with distinctive chemotherapeutic treatments, sICD enhances therapeutic efficacy ...
https://www.physiciansweekly.com/synthetic-immunogenic-cell-death-mediated-by-intracellular-delivery-of-sting-agonist-nanoshells-enhances-anti-cancer-chemo-immunotherapy/
Immunogenic cell death of dendritic cells following modified vaccinia virus Ankara infection enhances CD8(+) T cell...Immunogenic cell death of dendritic cells following modified vaccinia virus Ankara infection enhances CD8(+) T cell...
Immunogenic cell death (ICD) is associated with the emission of so-called damage-associated molecular patterns (DAMPs) which trigger the immune response against dead-cell associated antigens. The secretion of the DAMP, adenosine triphosphate (ATP) has been shown to be autophagy-dependent. Here, we demonstrate that Modified Vaccinia virus Ankara (MVA), a highly attenuated strain of vaccinia virus, induces both cell death and autophagy in murine bone marrow-derived dendritic cells (BMDCs), which in turn confer the (cross-)priming of OVA-specific cytotoxic T cells (OT-I cells). Additionally, we show that MVA infection leads to increased extracellular ATP (eATP) as well as intracellular ATP (iATP) levels, with the latter being influenced by the autophagy. Furthermore, we show that the increased eATP supports the proliferation of OT-I cells and inhibition of the P2RX7 receptors results in an abrogation of the proliferation. These data reveal novel mechanisms on how MVA enhances adaptive immunity in ...
https://epub.ub.uni-muenchen.de/65905/
Does native Trypanosoma cruzi calreticulin mediate growth inhibition of a mammary tumor during infection? | BMC Cancer | Full...Does native Trypanosoma cruzi calreticulin mediate growth inhibition of a mammary tumor during infection? | BMC Cancer | Full...
We have proposed that rTcCRT [13, 14] provides an important at least partial explanation of why T. cruzi experimental infection or the inoculation of parasite extracts exerts toxic effects on different tumor types, mammary adenocarcinoma among them (reviewed in [2]).. We have described a 45 kDa T. cruzi, highly pleiotropic chaperone protein, TcCRT [13]. rTcCRT [13] is antiangiogenic because it inhibits ECs proliferation, migration and morphogenesis, in several in vitro, ex vivo and in vivo assays, in 3 species, H. s. sapiens included [9, 16, 17]. Furthermore, in vivo, rTcCRT inhibits the growth of an experimental mammary adenocarcinoma, when inoculated in an area near the tumor. This effect is more potent than the one elicited by its rHuCRT counterpart [9]. However, the proposal that T.cruzi infection has an antitumor effect mediated by its nTcCRT, needs a formal demonstration, as justified in the Introduction section. This important question was addressed herein. In order to causally implicate ...
https://bmccancer.biomedcentral.com/articles/10.1186/s12885-016-2764-5
An Immunosurveillance Mechanism Controls Cancer Cell Ploidy | ScienceAn Immunosurveillance Mechanism Controls Cancer Cell Ploidy | Science
The surface exposure of CRT and ERp57 was increased in CT26 clones derived from cells transiently exposed to nocodazole that contained close to twice the DNA content of parental cells (which we refer to as hyperploid cells), although the surface expression of most other membrane proteins was unaltered (Fig. 1D and fig. S8). This hyperploidy-associated increase in CRT exposure was also observed in mouse Lewis lung carcinoma (LLC) and fibrosarcoma MCA205 cells, as well as in human cancer cell lines (fig. S9). As compared to their parental counterparts, hyperploid clones exhibited constitutive PERK and eIF2α phosphorylation (Fig. 1E). Interruption of the CRT exposure pathway reduced the clonogenic potential of hyperploid cells (Fig. 1, F and G), suggesting a functional link between the ER stress-associated CRT exposure pathway and the fitness of hyperploid cells.. Because hyperploidization is linked to CRT exposure, we wondered whether cancer cells with increased DNA content might be subjected ...
https://science.sciencemag.org/content/337/6102/1678?ijkey=b7624738446c27f42f11609bb55fc94c82de16b1&keytype2=tf_ipsecsha
An Immunosurveillance Mechanism Controls Cancer Cell Ploidy | ScienceAn Immunosurveillance Mechanism Controls Cancer Cell Ploidy | Science
The surface exposure of CRT and ERp57 was increased in CT26 clones derived from cells transiently exposed to nocodazole that contained close to twice the DNA content of parental cells (which we refer to as hyperploid cells), although the surface expression of most other membrane proteins was unaltered (Fig. 1D and fig. S8). This hyperploidy-associated increase in CRT exposure was also observed in mouse Lewis lung carcinoma (LLC) and fibrosarcoma MCA205 cells, as well as in human cancer cell lines (fig. S9). As compared to their parental counterparts, hyperploid clones exhibited constitutive PERK and eIF2α phosphorylation (Fig. 1E). Interruption of the CRT exposure pathway reduced the clonogenic potential of hyperploid cells (Fig. 1, F and G), suggesting a functional link between the ER stress-associated CRT exposure pathway and the fitness of hyperploid cells.. Because hyperploidization is linked to CRT exposure, we wondered whether cancer cells with increased DNA content might be subjected ...
https://science.sciencemag.org/content/337/6102/1678?ijkey=0aa37da628435da5c482b2cc9400649867e5f56c&keytype2=tf_ipsecsha
Caspase-2 and oxidative stress underlie the immunogenic potential of high hydrostatic pressure-induced cancer cell death -...
High hydrostatic pressure (HHP) promotes key characteristics of immunogenic cell death (ICD), in thus far resembling immunogenic chemotherapy and ionizing irradiation. Here, we demonstrate that cancer cells succumbing to HHP induce CD4+ and CD8+ T cell-dependent protective immunity in vivo. Moreover, we show that cell death induction by HHP relies on the overproduction of reactive oxygen species (ROS), causing rapid establishment of the integrated stress response, eIF2α phosphorylation by PERK, and sequential caspase-2, -8 and -3 activation. Non-phosphorylatable eIF2α, depletion of PERK, caspase-2 or -8 compromised calreticulin exposure by cancer cells succumbing to HHP but could not inhibit death. Interestingly, the phagocytosis of HHP-treated malignant cells by dendritic cells was suppressed by the knockdown of caspase-2 in the former. Thus, caspase-2 mediates a key function in the interaction between dying cancer cells and antigen presenting cells. Our results indicate that the ROS→PERK→eIF2α
https://www.hal.inserm.fr/inserm-01819520
Endoplasmic Reticulum and cis-Golgi Localization of Human T-Lymphotropic Virus Type 1 p12I: Association with Calreticulin and...Endoplasmic Reticulum and cis-Golgi Localization of Human T-Lymphotropic Virus Type 1 p12I: Association with Calreticulin and...
In this study, we determined the subcellular accumulation of HTLV-1 p12I to further define the possible function for this protein in viral infection. We demonstrate that p12I is retained as a membrane-associated protein and is expressed predominantly in the ER and cis-Golgi apparatus. Two regions of the protein containing predicted transmembrane domains are independently responsible for this pattern of localization. Importantly, we are the first to identify the interaction of p12I with both calreticulin and calnexin in the ER, suggesting a possible function of the viral protein in calcium-mediated cell signaling leading to host cell activation. These findings are consistent with our previous studies that demonstrated a requirement for HTLV-1 p12I in viral infectivity both in a rabbit model of infection (12), in primary lymphocytes in vitro (1), and in calcium-dependent nuclear factor of activated T cells-mediated transcription (B. Albrecht et al., unpublished data).. HTLV-1 p12I is highly ...
https://jvi.asm.org/content/75/16/7672?ijkey=11b99d8c0a342bf480fb7958381bdd4b480fedd8&keytype2=tf_ipsecsha
Three Novel Calreticulin Mutations in Two Turkish Patients [Turk J Hematol]Three Novel Calreticulin Mutations in Two Turkish Patients [Turk J Hematol]
Veysel Sabri Han er, H seyin Tokg z, Serkan G ven , mran al kan, Murat B y kdo an. Three Novel Calreticulin Mutations in Two Turkish Patients. Turk J Hematol. 2017; 34(4): 360- ...
http://www.tjh.com.tr/jvi.aspx?pdir=tjh&plng=eng&un=TJH-16878
A 4-trifluoromethyl analogue of celecoxib inhibits arthritis by suppressing innate immune cell activation | Arthritis Research ...A 4-trifluoromethyl analogue of celecoxib inhibits arthritis by suppressing innate immune cell activation | Arthritis Research ...
In the present study we demonstrate, using arthritis models, that TFM-C, a celecoxib analogue with 205-fold lower COX-2-inhibitory activity, inhibits autoimmune disease. TFM-C differs from celecoxib by the substitution of the 4-methyl group by a trifluoromethyl group. This substitution drastically increases the IC50s for inhibition of COX1 (15 μM to ,100 μM for celecoxib and TFM-C, respectively) and COX2 (0.04 μM to 8.2 μM, respectively), but does not affect the apoptotic index measured in PC3 prostate cancer cells, indicating independence between structural requirements for COX-2 inhibition and apoptosis induction [36]. Celecoxib perturbs intracellular calcium by blocking ER Ca2+ ATPases, and this activity is shared with TFM-C [23, 37]. In a HEK293 recombinant cell system, this Ca2+ perturbation is associated with inhibition of secretion and altered intracellular interaction of IL-12 polypeptide chains with the ER chaperones calreticulin and ERp44, and results in the interception of IL-12 ...
https://arthritis-research.biomedcentral.com/articles/10.1186/ar3683
Calreticulin Is the Dominant Pro-Phagocytic Signal on Multiple Human Cancers and Is Counterbalanced by CD47 | Science...Calreticulin Is the Dominant Pro-Phagocytic Signal on Multiple Human Cancers and Is Counterbalanced by CD47 | Science...
Thank you for your interest in spreading the word about Science Translational Medicine.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
https://stm.sciencemag.org/content/2/63/63ra94.full
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Platinum-based chemotherapy produced a paradigm shift in the treating different cancers initially; nevertheless, the success of the agents may reach the top as researchers have got tried different mixture regimes in various trials without having major differences in the end results. is still significant research required to accomplish full understanding of these resistance mechanisms to overcome the ineffectiveness or toxicities of platinum compounds. It seems affordable in the current perspective when standard chemotherapeutic brokers exhibited immunogenic cell death and they are currently in use with monoclonal antibodies to revisit the platinum brokers pharmacology. This may discover new basis for combination chemotherapy with monoclonal antibodies which may buy AZD4547 improve the current malignancy treatments by opening new vistas for newer buy AZD4547 combination regimes with less toxicity and better efficacy. In this article we review the pharmacologies of both cisplatin and oxaliplatin ...
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New treatments for triple-negative breast cancer (TNBC) are urgently needed. Despite there being little evidence of clinical activity as single-agent therapies, we show that dual blockade of PI3Kα and CDK4/6 is synergistically effective against multiple RB1-wild-type TNBC models. Combined PI3Kα and CDK4/6 inhibition significantly increased apoptosis, cell-cycle arrest, and tumor immunogenicity and generated immunogenic cell death in human TNBC cell lines. Combination treatment also significantly improved disease control in human xenograft models compared with either monotherapy. Combined PI3Kα and CDK4/6 inhibition significantly increased tumor-infiltrating T-cell activation and cytotoxicity and decreased the frequency of immunosuppressive myeloid-derived suppressor cells in a syngeneic TNBC mouse model. Notably, combined PI3Kα and CDK4/6 inhibition, along with inhibition of immune checkpoints PD-1 and CTLA-4, induced complete and durable regressions (,1 year) of established TNBC tumors in ...
http://cancerres.aacrjournals.org/content/77/22/6340
ICD Components | Nano PublicationsICD Components | Nano Publications
NBTXR3 exposed to irradiation enhanced cancer cells destruction and immunogenic cell death compared to irradiation alone, suggesting a strong potential for transforming tumor into an effective in situ vaccine. This may contribute to transform cold tumor into hot tumor and effectively be combined with most of the immunotherapeutic agents across oncology. NBTXR3 is intended to be injected in the tumors. Spilling in the circulation may occur during product administration or, as expected, during tumor destruction, leading to steady trapping of NPs in the reticulo-endothelial system (liver and spleen). Clinically, it is unknown whether patients, previously treated with NPs, may show toxic signs when NPs are exposed (activation) to diagnosis imaging (computed tomography(CT)) of the liver.. Continuer la lecture…. ...
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Complete information for UNC119B gene (Protein Coding), Unc-119 Lipid Binding Chaperone B, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
https://www.genecards.org/cgi-bin/carddisp.pl?gene=UNC119B&keywords=GH12J120709&prefilter=genomic_location
CalreticulinCalreticulin
... is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds ... The term "Mobilferrin" is considered to be the same as calreticulin by some sources. Calreticulin binds to misfolded proteins ... Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin, but calreticulin is not a ... "Entrez Gene: CALR calreticulin". Nangalia J, Massie CE, Baxter EJ, Nice FL, Gundem G, Wedge DC, Avezov E, Li J, Kollmann K, ...
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Calreticulin protein familyCalreticulin protein family
This family includes Calreticulin, Calnexin and Camlegin. Michalak M, Milner RE, Burns K, Opas M (August 1992). "Calreticulin ... In molecular biology, the calreticulin protein family is a family of calcium-binding proteins. ...
https://en.wikipedia.org/wiki/Calreticulin_protein_family
Jasvinder K GambhirJasvinder K Gambhir
"Protein acyltransferase function of purified calreticulin. Part 1: Characterization of propionylation of protein utilizing ...
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Essential thrombocythemiaEssential thrombocythemia
In 2013, two groups detected calreticulin mutations in a majority of JAK2-negative/MPL-negative patients with essential ... "Somatic mutations of calreticulin in myeloproliferative neoplasms". The New England Journal of Medicine. 369 (25): 2379-90. doi ...
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Perforin-1Perforin-1
July 1998). "Interaction between a Ca2+-binding protein calreticulin and perforin, a component of the cytotoxic T-cell granules ... 1998). "Interaction between a Ca2+-binding protein calreticulin and perforin, a component of the cytotoxic T-cell granules". ... Perforin has been shown to interact with calreticulin. Granzymes Defensin Complement membrane attack complex GRCh38: Ensembl ... perforin molecules translocate to the target cell with the help of calreticulin, which works as a chaperone protein to prevent ...
https://en.wikipedia.org/wiki/Perforin-1
ACAMPsACAMPs
Another externalized molecule marking apoptotic cells is calreticulin. Generally, the ability of apoptotic cells to change ... interacts with calreticulin which is a known C1q receptor), or complement receptors (CR3 and CR4). There is a variety of ... altered surface sugars on apoptotic cell and enable easier uptake by phagocytes which recognize their complex with calreticulin ...
https://en.wikipedia.org/wiki/ACAMPs
Avadhesha SuroliaAvadhesha Surolia
He elucidated the molecular features of substrate recognition by endoplasmic reticulum chaperones calreticulin and calnexin. He ... "Interactions of substrate with calreticulin, an endoplasmic reticulum chaperone". Journal of Biological Chemistry. 278 (8): ... "Isothermal titration calorimetric study defines the substrate binding residues of calreticulin". Biochemical and Biophysical ...
https://en.wikipedia.org/wiki/Avadhesha_Surolia
Cancer immunologyCancer immunology
"Calreticulin exposure dictates the immunogenicity of cancer cell death". Nature Medicine. 13 (1): 54-61. doi:10.1038/nm1523. ...
https://en.wikipedia.org/wiki/Cancer_immunology
Indolent T cell lymphoproliferative disorder of the gastrointestinal tractIndolent T cell lymphoproliferative disorder of the gastrointestinal tract
Cahu X, Constantinescu SN (December 2015). "Oncogenic Drivers in Myeloproliferative Neoplasms: From JAK2 to Calreticulin ...
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MyosinMyosin
... VIII and calreticulin as molecular determinants of sink strength?". Plant Physiology. 126 (1): 39-46. doi:10.1104/pp. ...
https://en.wikipedia.org/wiki/Myosin
CalnexinCalnexin
After the β2-microglobulin binds to the MHC class I peptide-loading complex (PLC), calreticulin and ERp57 take over the job of ... Oliver JD, Hresko RC, Mueckler M, High S (Jun 1996). "The glut 1 glucose transporter interacts with calnexin and calreticulin ... Del Bem LE (Feb 2011). "The evolutionary history of calreticulin and calnexin genes in green plants". Genetica. 139 (2): 225-9 ... Otteken A, Moss B (Jan 1996). "Calreticulin interacts with newly synthesized human immunodeficiency virus type 1 envelope ...
https://en.wikipedia.org/wiki/Calnexin
Mona NemerMona Nemer
"Modulation of gene expression by calreticulin binding to the glucocorticoid receptor". Nature. 367 (6462): 476-480. doi:10.1038 ...
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ArginylationArginylation
Carpio MA, Decca MB, Lopez Sambrooks C, Durand ES, Montich GG, Hallak ME (July 2013). "Calreticulin-dimerization induced by ... López Sambrooks C, Carpio MA, Hallak ME (June 2012). "Arginylated calreticulin at plasma membrane increases susceptibility of ... Another protein which benefits from arginylation is calreticulin because when modified, its role during endoplasmic reticulum ...
https://en.wikipedia.org/wiki/Arginylation
NK2 homeobox 1NK2 homeobox 1
... has been shown to interact with Calreticulin and PAX8. GRCh38: Ensembl release 89: ENSG00000136352 - Ensembl, ... Perrone L, Tell G, Di Lauro R (February 1999). "Calreticulin enhances the transcriptional activity of thyroid transcription ... Perrone L, Tell G, Di Lauro R (February 1999). "Calreticulin enhances the transcriptional activity of thyroid transcription ...
https://en.wikipedia.org/wiki/NK2_homeobox_1
Surfactant protein A2Surfactant protein A2
... cell surface calreticulin)". Immunopharmacology. 38 (1-2): 73-80. doi:10.1016/S0162-3109(97)00076-3. PMID 9476117. Karinch AM, ...
https://en.wikipedia.org/wiki/Surfactant_protein_A2
CD93CD93
"Calreticulin is released from activated neutrophils and binds to C1q and mannan-binding protein". Clinical Immunology and ... cell surface calreticulin)". Immunopharmacology. 38 (1-2): 73-80. doi:10.1016/S0162-3109(97)00076-3. PMID 9476117. Nepomuceno ...
https://en.wikipedia.org/wiki/CD93
Santa Cruz BiotechnologySanta Cruz Biotechnology
"Adiponectin modulates inflammatory reactions via calreticulin receptor-dependent clearance of early apoptotic bodies". J Clin ...
https://en.wikipedia.org/wiki/Santa_Cruz_Biotechnology
LRP1LRP1
... and calreticulin". Immunity. 14 (3): 303-13. doi:10.1016/s1074-7613(01)00111-x. PMID 11290339. Williams SE, Inoue I, Tran H, ... "Low density lipoprotein receptor-related protein is a calreticulin coreceptor that signals focal adhesion disassembly". The ...
https://en.wikipedia.org/wiki/LRP1
PDIA3PDIA3
This protein of the endoplasmic reticulum interacts with lectin chaperones such as calreticulin and CNX in order to modulate ... This protein localizes to the endoplasmic reticulum (ER) and interacts with lectin chaperones calreticulin and calnexin (CNX) ... and polypeptide binding sites of calnexin and calreticulin". The Journal of Biological Chemistry. 277 (33): 29686-97. doi: ...
https://en.wikipedia.org/wiki/PDIA3
Stress granuleStress granule
"Post-translational arginylation of calreticulin: a new isospecies of calreticulin component of stress granules". The Journal of ...
https://en.wikipedia.org/wiki/Stress_granule
TapasinTapasin
The peptide-loading complex consists of TAP, tapasin, MHC class I, calreticulin, and ERp57. Tapasin recruits MHC class I ... Sadasivan B, Lehner PJ, Ortmann B, Spies T, Cresswell P (August 1996). "Roles for calreticulin and a novel glycoprotein, ... Sadasivan B, Lehner PJ, Ortmann B, Spies T, Cresswell P (August 1996). "Roles for calreticulin and a novel glycoprotein, ...
https://en.wikipedia.org/wiki/Tapasin
Chaperone (protein)Chaperone (protein)
Lectin chaperones: calnexin and calreticulin Non-classical molecular chaperones: HSP47 and ERp29 Folding chaperones: Protein ... "TROSY-NMR reveals interaction between ERp57 and the tip of the calreticulin P-domain". Proceedings of the National Academy of ...
https://en.wikipedia.org/wiki/Chaperone_(protein)
Cortical granuleCortical granule
Calreticulin: Evidence has indicated the presence of the protein, calreticulin, within the cortical granule. Researchers have ... On the other hand, different research has shown that calreticulin may be released from vesicles other than cortical granules. ... Furthermore, upon exocytosis, this calreticulin interacts with the oocyte's cytoskeleton, thereby allowing the transmission of ... Additionally contributing to polyspermy prevention, calreticulin may also inhibit certain glycoproteins, which promote ...
https://en.wikipedia.org/wiki/Cortical_granule
Calcitonin receptorCalcitonin receptor
The network of endoplasmic reticulum-resident chaperones (ERp72, GRP94, calreticulin, and BiP) interacts with apolipoprotein b ... calreticulin, and cyclophilin B". The Journal of Biological Chemistry. 278 (9): 7459-68. doi:10.1074/jbc.M207976200. PMID ... "C1q and mannose binding lectin engagement of cell surface calreticulin and CD91 initiates macropinocytosis and uptake of ... "Low density lipoprotein receptor-related protein is a calreticulin coreceptor that signals focal adhesion disassembly". The ...
https://en.wikipedia.org/wiki/Calcitonin_receptor
Intracellular calcium-sensing proteinsIntracellular calcium-sensing proteins
Examples include: calmodulin calnexin calreticulin gelsolin Ghoshdastider U, Popp D, Burtnick LD, Robinson RC (2013). "The ...
https://en.wikipedia.org/wiki/Intracellular_calcium-sensing_proteins
CALRCALR
... may refer to: Calreticulin, a protein that in humans is encoded by the CALR gene. Chief Albert Luthuli Regiment, an ...
https://en.wikipedia.org/wiki/CALR
PPIBPPIB
In the ER, PPIB interacts with proteins such as P3H1, CRTAP, BiP, GRP94, PDI, and calreticulin to form foldase and chaperone ... PPIB has been shown to interact with: Apolipoprotein B. P3H1, CRTAP, BiP, GRP94, PDI, and calreticulin. GRCh38: Ensembl release ... calreticulin, and cyclophilin B". J. Biol. Chem. 278 (9): 7459-68. doi:10.1074/jbc.M207976200. PMID 12397072. Rasmussen HH, van ... is retained intracellularly via a unique COOH-terminal sequence and colocalizes with the calcium storage protein calreticulin ...
https://en.wikipedia.org/wiki/PPIB
PhlebovirusPhlebovirus
At the same time, calreticulin prevents any misfolded Gn/Gc from being exported to the Golgi. Sixth, The correctly folded Gn/Gc ...
https://en.wikipedia.org/wiki/Phlebovirus
CD47CD47
High levels of CD47 allows cancer cells to avoid phagocytosis despite having a higher level of calreticulin - the dominant pro- ... "Calreticulin is the dominant pro-phagocytic signal on multiple human cancers and is counterbalanced by CD47". Sci Transl Med. 2 ...
https://en.wikipedia.org/wiki/CD47
Peptide loading complexPeptide loading complex
Calreticulin is crucial and is highly dependant in the assembly and maturation of MHC-I in the PLC. The globular lectin domain ... When MHC-I chains are empty, they are recruited by calreticulin and form a transient PLC. Tapasin regularly plays a role in the ... Preliminary MHC-I heavy chains form chaperones with the aid of the calnexin-calreticulin complex in the ER. In addition to this ... In general, preliminary MHC-I heavy chains are chaperoned by the calnexin-calreticulin system in the ER. Together with β2- ...
https://en.wikipedia.org/wiki/Peptide_loading_complex
Calreticulin variant stratified driver mutational status and prognosis in essential thrombocythemia - Fingerprint - Mayo...
Calreticulin variant stratified driver mutational status and prognosis in essential thrombocythemia. Yoseph C. Elala, Terra L. ... Dive into the research topics of Calreticulin variant stratified driver mutational status and prognosis in essential ...
https://mayoclinic.pure.elsevier.com/en/publications/calreticulin-variant-stratified-driver-mutational-status-and-prog/fingerprints/
Does native Trypanosoma cruzi calreticulin mediate growth inhibition of a mammary tumor during infection? | BMC Cancer | Full...Does native Trypanosoma cruzi calreticulin mediate growth inhibition of a mammary tumor during infection? | BMC Cancer | Full...
The molecular basis of this phenomenon remained basically unknown until our proposal that T. cruzi Calreticulin (TcCRT), an ... Molina MC, Ferreira V, Valck C, Aguilar L, Orellana J, Rojas A, et al. An in vivo role for Trypanosoma cruzi calreticulin in ... Abello-Cáceres, P., Pizarro-Bauerle, J., Rosas, C. et al. Does native Trypanosoma cruzi calreticulin mediate growth inhibition ... Trypanosoma cruzi calreticulin inhibits the complement lectin pathway activation by direct interaction with L-Ficolin. Mol ...
https://bmccancer.biomedcentral.com/articles/10.1186/s12885-016-2764-5
Regulation of peripheral T cell activation by calreticulin | Journal of Cell Biology | Rockefeller University PressRegulation of peripheral T cell activation by calreticulin | Journal of Cell Biology | Rockefeller University Press
Regulation of peripheral T cell activation by calreticulin Simona Porcellini, Simona Porcellini ... Regulation of peripheral T cell activation by calreticulin . J Cell Biol 27 February 2006; 172 (5): i11. doi: https://doi.org/ ...
https://rupress.org/jcb/article/172/5/i11/44225/Regulation-of-peripheral-T-cell-activation-by
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CRT3 / Anti-Calreticulin-3 Antibody
Anti-Calreticulin-3 Antibody Product Information Form: Lyophilized Stability & Storage: Use a manual defrost freezer and avoid ... Youre reviewing:CRT3 / Anti-Calreticulin-3 Antibody. Your Rating. Rating. 1 star 2 stars 3 stars 4 stars 5 stars ... Calreticulin (CRT) is an endoplasmic reticulum (ER)-localized chaperone-like lectin that plays a crucial role in promoting the ... CRT3, CALRETICULIN 3, EBS2, EMS-MUTAGENIZED BRI1 SUPPRESSOR 2, PRIORITY IN SWEET LIFE 1, PSL1. ...
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Calreticulin is present in the acrosome of spermatids of rat testis - Fingerprint - Waseda University
Calreticulin is present in the acrosome of spermatids of rat testis. Masahisa Nakamura*, Yuichi Michikawa, Tadashi Baba, ... Dive into the research topics of Calreticulin is present in the acrosome of spermatids of rat testis. Together they form a ...
https://waseda.pure.elsevier.com/en/publications/calreticulin-is-present-in-the-acrosome-of-spermatids-of-rat-test/fingerprints/
CAL2 monoclonal antibody is a rapid and sensitive assay for the detection of calreticulin mutations in essential...CAL2 monoclonal antibody is a rapid and sensitive assay for the detection of calreticulin mutations in essential...
Calreticulin (CALR) mutations are detected in the majority of JAK2 wild type patients with essential thrombocythemia (ET). ... Calreticulin (CALR) mutations are detected in the majority of JAK2 wild type patients with essential thrombocythemia (ET). ... CAL2 monoclonal antibody is a rapid and sensitive assay for the detection of calreticulin mutations in essential ...
https://iris.univr.it/handle/11562/996750
Cancer biology thematic collection: Cell ReportsCancer biology thematic collection: Cell Reports
... with mutations in the endoplasmic reticulum chaperone calreticulin. They show that calreticulin mutations perturb the ... Calreticulin mutations affect its chaperone function and perturb the glycoproteome. Cell Reports ... structural landscape of the glycoproteome and provide molecular proof of protein misfolding in calreticulin-mutated MPNs. ...
https://www.cell.com/cell-reports/collections/topic-cancer?rel=nofollow
The ATF6β-calreticulin axis promotes neuronal survival under endoplasmic reticulum stress and excitotoxicity - Ishii, Hiroshi -...
The ATF6β-calreticulin axis promotes neuronal survival under endoplasmic reticulum stress and excitotoxicity ... The ATF6β-calreticulin axis promotes neuronal survival under endoplasmic reticulum stress and excitotoxicity ... and specifically regulates the expression of calreticulin (CRT), a molecular chaperone in the ER with a high Ca2+-binding ...
https://repo.lib.tokushima-u.ac.jp/en/list/author-page/Hiroshi,Ishii/item/116629
A physical interaction network of dengue virus and human proteinsA physical interaction network of dengue virus and human proteins
We further showed that calreticulin colocalized with viral dsRNA and with the viral NS3 and NS5 proteins in DENV-infected cells ... consistent with a direct role for calreticulin in DENV replication. Human proteins that interacted with DENV had significantly ...
https://pubmed.ncbi.nlm.nih.gov/21911577/
Proteomes | Free Full-Text | Differential Proteome Analysis of Extracellular Vesicles from Breast Cancer Cell Lines by...Proteomes | Free Full-Text | Differential Proteome Analysis of Extracellular Vesicles from Breast Cancer Cell Lines by...
Zamanian, M.; Qader Hamadneh, L.A.; Veerakumarasivam, A.; Abdul Rahman, S.; Shohaimi, S.; Rosli, R. Calreticulin mediates an ...
https://www.mdpi.com/2227-7382/5/4/25
A large-scale nanoscopy and biochemistry analysis of postsynaptic dendritic spines | Nature NeuroscienceA large-scale nanoscopy and biochemistry analysis of postsynaptic dendritic spines | Nature Neuroscience
Calreticulin, a calcium-binding molecular chaperone, is required for stress response and fertility in Caenorhabditis elegans. ... Functional specialization of calreticulin domains. J. Cell Biol. 154, 961-972 (2001). ...
https://www.nature.com/articles/s41593-021-00874-w?error=cookies_not_supported&code=902fcd9b-c5f5-44a5-b0aa-bbd7326f71cd
CATH Superfamily 2.10.250.10CATH Superfamily 2.10.250.10
Calreticulin/calnexin, P domain. The name of this superfamily has been modified since the most recent official CATH+ release ( ...
http://www.cathdb.info/version/v4_3_0/superfamily/2.10.250.10/superposition
Melatonin as an Anti-Inflammatory Agent Modulating Inflammasome ActivationMelatonin as an Anti-Inflammatory Agent Modulating Inflammasome Activation
M. Macias, G. Escames, J. León et al., "Calreticulin-melatonin. An unexpected relationship," European Journal of Biochemistry, ... Melatonin may also interact with cytosolic proteins including calmodulin and calreticulin, which are involved in the ...
https://www.hindawi.com/journals/ije/2017/1835195/
Endogenous megakaryocytic colonies underline association between megakaryocytes and calreticulin mutations in Essential...Endogenous megakaryocytic colonies underline association between megakaryocytes and calreticulin mutations in Essential...
Mondet, J. et al. Endogenous megakaryocytic colonies underline association between megakaryocytes and calreticulin mutations in ...
http://www.labex-grex.com/publications/endogenous-megakaryocytic-colonies-underline-association-between-megakaryocytes-and-calreticulin-mutations-in-essential-thrombocythemia/
Developmental BiologyDevelopmental Biology
Calreticulin regulates a switch between osteoblast and chondrocyte lineages derived from murine embryonic stem cells. Journal ... Calreticulin is a highly conserved, ubiquitous Ca2+-buffering protein in the endoplasmic reticulum that controls ... Calreticulin activates calcineurin, which dephosphorylates and induces the nuclear import of the osteogenic transcription ... regulator nuclear factor of activated T cells 1 (NFATC1). We investigated whether calreticulin controls a switch between ...
https://www.jbc.org/Developmental-Biology?startPage=&KeywordRaw=gene%20regulation
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Calreticulin mutant myeloproliferative neoplasms induce MHC-I skewing, which can be overcome by an optimized peptide cancer...
Calreticulin mutant myeloproliferative neoplasms induce MHC-I skewing, which can be overcome by an optimized peptide cancer ... Calreticulin mutant myeloproliferative neoplasms induce MHC-I skewing, which can be overcome by an optimized peptide cancer ... "Calreticulin mutant myeloproliferative neoplasms induce MHC-I skewing, which can be overcome by an optimized peptide cancer ... Calreticulin mutant myeloproliferative neoplasms induce MHC-I skewing, which can be overcome by an optimized peptide cancer ...
https://research.regionh.dk/da/publications/calreticulin-mutant-myeloproliferative-neoplasms-induce-mhci-skewing-which-can-be-overcome-by-an-optimized-peptide-cancer-vaccine
A calreticulin-like molecule from the human hookworm Necator americanus interacts with C1q and the cytoplasmic signalling...
As human calreticulin has been shown to bind to and neutralize the haemolytic activity of the complement component C1q, and to ... As human calreticulin has been shown to bind to and neutralize the haemolytic activity of the complement component C1q, and to ... As human calreticulin has been shown to bind to and neutralize the haemolytic activity of the complement component C1q, and to ... As human calreticulin has been shown to bind to and neutralize the haemolytic activity of the complement component C1q, and to ...
https://researchportal.lih.lu/en/publications/a-calreticulin-like-molecule-from-the-human-hookworm-necator-amer
Mutation in the intracellular chloride channel CLCC1 associated with autosomal recessive retinitis pigmentosa | PLOS GeneticsMutation in the intracellular chloride channel CLCC1 associated with autosomal recessive retinitis pigmentosa | PLOS Genetics
CLCC1 WT and p.D25E are both shown colocalised with calreticulin. Both WT and mutant proteins localize with ER (a-f), and with ... CLCC1 does not contain a KLGFFKR sequence, to which Calreticulin is known to bind, suggesting that this binding is likely ... Co-immunoprecipitation and western blotting showed that both WT and mutant CLCC1 interacted with Calreticulin, a major calcium ... Proteins were immobilized on the PVDF membrane (Millipore). Antibodies (CLCC1 1:1000, Calreticulin 1:1000, GAPDH (MA5-15738, ...
https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007504
Internet Scientific Publications
Calreticulin is also a calcium-binding protein. It has been shown to promote many cellular functions, such as supporting the ... Calreticulin can be consumed through milk and milk products, which also provide calcium. Unfortunately, the protein in dairy ...
http://ispub.com/IJNW/1/2/12874
Subject: Glycine max and citrates / Subject term: citrates - PubAg Search ResultsSubject: Glycine max and citrates / Subject term: citrates - PubAg Search Results
Rheumatism: Disease Bioinformatics: Novus BiologicalsRheumatism: Disease Bioinformatics: Novus Biologicals
Calreticulin Antibody - BSA Free. NB600-101. Rabbit Polyclonal Species Human, Mouse, Rat. Applications WB, Simple Western, DB ...
https://www.novusbio.com/diseases/rheumatism
Second near-infrared photothermal-amplified immunotherapy using photoactivatable composite nanostimulators | Research SquareSecond near-infrared photothermal-amplified immunotherapy using photoactivatable composite nanostimulators | Research Square
Exposure of the tumor cells to calreticulin (CRT) as a specific signal for triggering APCs was a characteristic sign of ICD ... calreticulin; MFI: mean fluorescence intensity; BMDCs: bone marrow-derived dendritic cells; IL-6: interleukin-6; TNF-α: tumor ...
https://www.researchsquare.com/article/rs-889559/v1
Publications | CrickPublications | Crick
Calreticulin is a secreted BMP antagonist, expressed in Hensens node during neural induction. Authors (6)* ...
https://www.crick.ac.uk/research/publications?f%5B0%5D=publication_type_latest_pub%3A429&f%5B1%5D=topic%3A29&f%5B2%5D=year_published_latest_pub%3A2016
Frontiers | Reactive Oxygen Species in Anticancer Immunity: A Double-Edged SwordFrontiers | Reactive Oxygen Species in Anticancer Immunity: A Double-Edged Sword
2012). A Novel Pathway Combining Calreticulin Exposure and ATP Secretion in Immunogenic Cancer Cell Death. EMBO J. 31, 1062- ... The induction of endoplasmic reticulum (ER) stress, surface exposure of calreticulin, and secretion of adenosine triphosphate ( ... The display of calreticulin was crucial by providing the motifs needed for the engulfment of PDT-treated cells by dendritic ... which then led to calreticulin exposure and release of HMGB1 and ATP to trigger ICD (Bugaut et al., 2013). Actually, many ...
https://www.frontiersin.org/articles/10.3389/fbioe.2021.784612/full
Search results - ORA - Oxford University Research ArchiveSearch results - ORA - Oxford University Research Archive
The role of calnexin, calreticulin and heavy chain glycosylation in MHC class I assembly ...
https://ora.ox.ac.uk/objects/?f%5Bf_subject%5D%5B%5D=Glycosylation
Accumulation of prohibitin is a common cellular response to different stressing stimuli and protects melanoma cells from ER...Accumulation of prohibitin is a common cellular response to different stressing stimuli and protects melanoma cells from ER...
https://doi.org/10.18632/oncotarget.17810 Tharcisio Citrangulo Tortelli Junior, Lyris Martins Franco de Godoy, Gustavo Antonio de Souza, Diego Bonatto, Andreia Hanada Otake, Renata de Freitas Saito,...
https://www.oncotarget.com/article/17810/text/
The rate of transformation from JAK2-mutated ET to PV is influenced by an accurate WHO-defined clinico-morphological diagnosis ...The rate of transformation from JAK2-mutated ET to PV is influenced by an accurate WHO-defined clinico-morphological diagnosis ...
LONG-TERM SURVIVAL; ESSENTIAL THROMBOCYTHEMIA; MYELOPROLIFERATIVE NEOPLASMS; POLYCYTHEMIA-VERA; CALRETICULIN MUTATIONS; ...
http://kups.ub.uni-koeln.de/40347/
Circulating Protein Disulfide Isomerase Is Associated with Increased Risk of Thrombosis in JAK2-Mutated Myeloproliferative...Circulating Protein Disulfide Isomerase Is Associated with Increased Risk of Thrombosis in JAK2-Mutated Myeloproliferative...
... as mutant calreticulin functions exclusively through MPL, the thrombopoietin receptor (41). ...
https://aacrjournals.org/clincancerres/article/27/20/5708/671725/Circulating-Protein-Disulfide-Isomerase-Is
CALRMyeloproliferative neoplasmsMutations in essential thrombocythemiaCalnexinRecombinantEndoplasmic reticulum-residentProteinAntibodyProteinsSpecificityMolecularGeneExpressionEndogenousMyelofibrosisCalciumEssentialRoleInfectionCellFoundPatientsHostFunctionsEndoplasmic reticulumAntibodyErp57Binding site of calreticulinAnalysis of CalreticulinMutations in calreticulinIntracellularAntigenNeoplasmsTumorExposureInhibitsChaperoneGeneSolubleMaturationGenes2017IsoformsImmuneAssaysMacrophagesAntibodiesReceptorCalcium homeostasisInductionSuperfamilyAffectsIncreasesConcentrationSyntheticStore-operatedRabbit
CALR5
  • The CALR gene provides instructions for making a multi-functional protein called calreticulin. (medlineplus.gov)
  • Calreticulin (CALR) mutations are detected in the majority of JAK2 wild type patients with essential thrombocythemia (ET). (univr.it)
  • abstract = "The majority of JAK2V617F-negative myeloproliferative neoplasms (MPNs) have disease-initiating frameshift mutations in calreticulin (CALR), resulting in a common carboxyl-terminal mutant fragment (CALRMUT), representing an attractive source of neoantigens for cancer vaccines. (regionh.dk)
  • This usually incorporates mutations in Janus kinase 2 (JAK2), MPL, and calreticulin (CALR) genes. (who.int)
  • The majority of patients with essential thrombocytosis have mutations in one of three genes: Janus kinase 2 (JAK2), calreticulin (CALR), or myeloproliferative leukemia virus oncogene (MPL). (medscape.com)
Myeloproliferative neoplasms1
  • apply limited proteolysis-coupled mass spectrometry to assess structural perturbations of the proteome in myeloproliferative neoplasms (MPNs) with mutations in the endoplasmic reticulum chaperone calreticulin. (cell.com)
Mutations in essential thrombocythemia1
  • Endogenous megakaryocytic colonies underline association between megakaryocytes and calreticulin mutations in Essential thrombocythemia. (labex-grex.com)
Calnexin2
  • In the present study, we HLA-genotyped and identified at a biochemical level the three (HLA-A25, -B8, -Cw7) class I alleles expressed by the previously described β 2 m-defective human melanoma FO-1 cell line and tested their ability to interact with calnexin, calreticulin and the TAP (transporter associated with antigen processing) complex. (elsevier.com)
  • All these alleles were found to bind calnexin, but not calreticulin or the poorly expressed TAP complex, both in parental and β 2 m-transfected FO-1 cells, demonstrating a complex defect of class I expression in FO-1 cells. (elsevier.com)
Recombinant4
  • We can now report that recombinant calreticulin failed to demonstrate significant calcium binding capacity, which is a hallmark of calreticulins in general and may indicate inappropriate folding following expression in a prokaryote. (lih.lu)
  • Nevertheless, recombinant calreticulin retained sufficient molecular architecture to bind to, and inhibit the haemolytic capacity of, human C1q. (lih.lu)
  • Furthermore, recombinant calreticulin reacted in surface plasmon resonance analysis (SPR) with peptides corresponding to cytoplasmic signalling domains of the integrins αIIb and α5, in a calcium independent manner. (lih.lu)
  • Calreticulin was identified by 2D electrophoresis and mass spectrometry as a glycoprotein that was bound specifically by recombinant S Ch FimH protein, but not by FimH from S E. (receptors.org)
Endoplasmic reticulum-resident1
  • The molecular basis of this phenomenon remained basically unknown until our proposal that T. cruzi Calreticulin (TcCRT), an endoplasmic reticulum-resident chaperone, translocated-externalized by the parasite, may mediate at least an important part of this effect. (biomedcentral.com)
Protein5
  • The ER is involved in protein processing and transport, and within this structure, calreticulin plays a role in ensuring the proper folding of newly formed proteins. (medlineplus.gov)
  • These genetic changes lead to production of an altered calreticulin protein with a different sequence of building blocks (amino acids) at one end. (medlineplus.gov)
  • The effect of the genetic changes on calreticulin function is unknown, and researchers are working to determine how the altered protein is involved in primary myelofibrosis. (medlineplus.gov)
  • They show that calreticulin mutations perturb the structural landscape of the glycoproteome and provide molecular proof of protein misfolding in calreticulin -mutated MPNs. (cell.com)
  • COS 7 cell expressing Organelle LightsTM ER-GFP (Invitrogen) consiting of the signal sequence of the ER protein calreticulin and a KDEL retention sequence. (cellimagelibrary.org)
Antibody1
  • SPR was also used to ratify the specificity of a polyclonal antibody to hookworm calreticulin, which was then used to assess the stage specificity of expression of the native molecule (in comparison with reverse transcriptase-polymerase chain reaction), to indicate its apparent secretion, and to purify native calreticulin from worm extracts by affinity chromatography. (lih.lu)
Proteins3
  • Calreticulin (CRT) is an endoplasmic reticulum (ER)-localized chaperone-like lectin that plays a crucial role in promoting the folding and maturation of newly synthesized glycoproteins and retaining incompletely/mis-folded proteins in the ER through its specific binding to monoglucosylated aspariginelinked glycans. (phytoab.com)
  • We further showed that calreticulin colocalized with viral dsRNA and with the viral NS3 and NS5 proteins in DENV-infected cells, consistent with a direct role for calreticulin in DENV replication. (nih.gov)
  • The functionality of calreticulin as a specific receptor of S Ch FimH adhesin was further confirmed by adhesion and invasion of mutated strains of S Ch carrying different variants of FimH proteins to IPEC-J2 cells with overexpression and silenced expression of calreticulin. (receptors.org)
Specificity1
  • Here we identify porcine calreticulin expressed by swine intestinal cells as a host-specific receptor for S Ch FimH adhesin, suggesting that such an interaction may contribute to S Ch host specificity. (receptors.org)
Molecular2
  • Here, we demonstrate that ATF6β is highly expressed in the hippocampus of the brain, and specifically regulates the expression of calreticulin (CRT), a molecular chaperone in the ER with a high Ca2+-binding capacity. (tokushima-u.ac.jp)
  • En el RETÍCULO ENDOPLÁSMICO se une a oligosacáridos con unión N específica que se encuentran en las proteínas recientemente sintetizadas y funciona como CHAPERONA MOLECULAR que puede desempeñar un papel en el PLEGAMIENTO DE PROTEÍNAS o retención y degradación de proteínas mal plegadas. (bvsalud.org)
Gene1
  • Through calcium regulation and other mechanisms, calreticulin is thought to play a role in the control of gene activity, cell growth and division (proliferation) and movement (migration), the attachment of cells to one another (adhesion), and regulation of programmed cell death (apoptosis). (medlineplus.gov)
Expression2
  • The Ca2+ Status of the Endoplasmic Reticulum Is Altered by Induction of Calreticulin Expression in Transgenic Plants. (mpg.de)
  • Moreover, binding of S Ch carrying the active variant of FimH to IPEC-J2 with silenced calreticulin expression was significantly weaker. (receptors.org)
Endogenous1
  • We evaluated the therapeutic efficacy of a novel endogenous inhibitor of angiogenesis, the calreticulin anti-angiogenic domain (CAD180), and its functional 112-residue fragment, CAD-like peptide 112 (CAD112), delivered using a self-complementary adeno-associated virus serotype 2 (scAAV2) in rodent models of oxygen-induced retinopathy and laser-induced choroidal neovascularization. (edu.au)
Myelofibrosis1
  • Homozygous calreticulin mutations in patients with myelofibrosis lead to acquired myeloperoxidase deficiency. (cdc.gov)
Calcium2
  • The ER is also a storage location for charged calcium atoms (calcium ions), and calreticulin is involved in maintaining the correct levels of calcium ions in this structure. (medlineplus.gov)
  • In addition calreticulin is a major storage form for CALCIUM and functions as a calcium-signaling molecule that can regulate intracellular calcium HOMEOSTASIS . (bvsalud.org)
Essential1
  • It is not clear how the alteration in calreticulin affects the protein's function or leads to the signs and symptoms of essential thrombocythemia. (medlineplus.gov)
Role2
  • This development will allow the functional tests described above to be repeated for native calreticulin, to ascertain its role in the host-parasite relationship. (lih.lu)
  • 2008). Protective role of calreticulin in HFE hemochromatosis . (up.pt)
Infection1
  • Does native Trypanosoma cruzi calreticulin mediate growth inhibition of a mammary tumor during infection? (biomedcentral.com)
Cell1
  • A long non-coding RNA links calreticulin-mediated immunogenic cell removal to RB1 transcription. (mpg.de)
Found1
  • It was found that S Ch carrying the active variant of FimH adhered and invaded IPEC-J2 cells with calreticulin overexpression at significantly higher numbers than those of SCh expressing the non-active variant or S E variant of FimH. (receptors.org)
Patients1
  • Calreticulin Mutations in Bulgarian MPN Patients. (cdc.gov)
Host1
  • Calreticulin was recently identified as a hookworm (Necator americanus) allergen, implying secretion, and contact with cells of the immune system, or significant worm attrition in the tissues of the host. (lih.lu)
Functions1
  • Calreticulin: non-endoplasmic reticulum functions in physiology and disease. (medlineplus.gov)
Endoplasmic reticulum9
  • Calreticulin (CRT) is a highly conserved multifunctional protein that was originally identified as a major calcium-binding protein of the endoplasmic reticulum [ 1 ]. (hindawi.com)
  • Calreticulin, an endoplasmic reticulum (ER) resident protein, affects many critical cellular functions, including protein folding and calcium homeostasis. (rupress.org)
  • Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. (cusabio.com)
  • Goat anti calreticulin antibody recognises calreticulin, a 418 aminoacid ~60 kDa Ca2+ binding chaperone involved in protein modification and folding within the endoplasmic reticulum (UniProt: P15253). (bio-rad-antibodies.com)
  • encodes a Ca2+ joining chaperone protein, calreticulin that localizes primarily to the endoplasmic reticulum (Emergency room) and regulates protein folding quality control pathways (15). (research-matters.net)
  • Calreticulin: non-endoplasmic reticulum functions in physiology and disease. (medlineplus.gov)
  • The Ca2+ Status of the Endoplasmic Reticulum Is Altered by Induction of Calreticulin Expression in Transgenic Plants. (mpg.de)
  • Cytoskeleton, apoptosis, protein synthesis, and the markers of endoplasmic reticulum stress including 78-kDa glucose-regulated protein and calreticulin in endothelial cells were examined. (stopumts.nl)
  • Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. (nih.gov)
Antibody5
  • Immunohistochemical analysis of calreticulin in pancreatic/ampullary tumor tissue arrays using an isoform nonspecific antibody revealed diffuse and consistent cytoplasmic staining in the neoplastic epithelial cells of the pancreatic and ampullary adenocarcinomas. (nih.gov)
  • Chen H, Wang G, Lu X. Anti-Calreticulin Antibody - a Novel Antibody in Patients with Idiopathic Inflammatory Myopathies and Its Association with Malignancy [abstract]. (acrabstracts.org)
  • Antibody levels to recombinant tick calreticulin increase in humans after exposure to Ixodes scapularis (Say) and are correlated with tick engorgement indices. (nih.gov)
  • The antibody responses of subjects who presented with a definite Ixodes scapularis (Say) tick bite were measured to determine the utility of the antibody response against recombinant tick calreticulin (rTC) as a biologic marker of tick exposure. (nih.gov)
  • Product Name: Human Calreticulin-3 (CALR3) Antibody (PerCP Conjugate)Description: Purified antibody validated for specificity and. (emlinhibitor.com)
Erp572
  • The PLC contains a peptide transporter (transporter associated with antigen processing), a thiooxido-reductase (ERp57), a glycoprotein chaperone (calreticulin), and tapasin, a class I-specific chaperone. (ox.ac.uk)
  • We suggest that tapasin is the key chaperone that directs this action of the PLC with secondary contributions from calreticulin and possibly ERp57. (ox.ac.uk)
Binding site of calreticulin1
  • Interaction took place through the hydrophobic C-terminus of Aβ 1- 42 and the polypeptide binding site of calreticulin. (eurekaselect.com)
Analysis of Calreticulin2
Mutations in calreticulin2
  • Somatic mutations in calreticulin (mutations induce MPN. (research-matters.net)
  • Acquired mutations in calreticulin or myeloproliferative leukemia virus oncogene are found in a significant number of patients with essential thrombocythemia or myelofibrosis, and mutations in numerous epigenetic regulators and spliceosome components are also seen. (haematologica.org)
Intracellular3
  • Levels of cell surface calreticulin (CRT) and intracellular ATP levels were evaluated using flow cytometry. (esmo.org)
  • In addition calreticulin is a major storage form for CALCIUM and functions as a calcium-signaling molecule that can regulate intracellular calcium HOMEOSTASIS . (bvsalud.org)
  • MO25 download handboek klinische ontwikkelingspsychologie over aanleg omgeving en verandering, BCAP, and CD19 are as residues which contain groups to the family % and mean intracellular defects, the methods. (erik-mill.de)
Antigen2
  • Antisera to calreticulin were raised by repeated immunisation of goats with highly purified antigen. (bio-rad-antibodies.com)
  • Radiation-induced immunogenic modulation of tumor enhances antigen processing and calreticulin exposure, resulting in enhanced T-cell killing. (nih.gov)
Neoplasms3
  • 12. Conformation sensitive gel electrophoresis for the detection of calreticulin mutations in BCR-ABL1-negative myeloproliferative neoplasms. (nih.gov)
  • 14. Calreticulin mutation specific CAL2 immunohistochemistry accurately identifies rare calreticulin mutations in myeloproliferative neoplasms. (nih.gov)
  • 20. Rare type 1-like and type 2-like calreticulin mutants induce similar myeloproliferative neoplasms as prevalent type 1 and 2 mutants in mice. (nih.gov)
Tumor2
  • Investigation for clinical usefulness of the novel urinary tumor marker, urinary calreticulin, in diagnosis for bladder cancer. (nii.ac.jp)
  • Specifically, the tumor STC1 traps a key eat-me signal called calreticulin, or CRT. (medicineinnovates.com)
Exposure1
  • M. Obeid, A. Tesniere, and F. Ghiringhelli , Calreticulin exposure dictates the immunogenicity of cancer cell death , Nature Medicine , vol. 279 , issue. (inserm.fr)
Inhibits1
  • Calreticulin inhibits adipogenesis via a negative feedback mechanism whereby the expression of calreticulin is initially up-regulated by peroxisome proliferator-activated receptor γ (PPARγ). (rupress.org)
Chaperone1
  • The expression of the chaperone calreticulin was up-regulated in cells with efficient B(2) receptor maturation. (uzh.ch)
Gene2
  • Through calcium regulation and other mechanisms, calreticulin is thought to play a role in the control of gene activity, cell growth and division (proliferation) and movement (migration), the attachment of cells to one another (adhesion), and regulation of programmed cell death (apoptosis). (medlineplus.gov)
  • Lenartowski R, Suwińska A , Prusińska J, Gumowski K, Lenartowska M (2014) Molecular cloning and transcriptional activity of a new Petunia calreticulin gene involved in pistil transmitting tract maturation, progamic phase, and double fertilization. (umk.pl)
Soluble1
  • 13. Surface-exposed and soluble calreticulin: conflicting biomarkers for cancer prognosis. (nih.gov)
Maturation2
  • Vice versa, upon down regulation of calreticulin expression by RNA interference, B(2) receptor maturation and AT(1)/B(2) receptor heterodimerization were significantly impaired. (uzh.ch)
  • Thus, calreticulin enhances B(2) receptor maturation and heterodimerization with AT(1). (uzh.ch)
Genes1
  • Other clonal mutations sometimes seen involve the calreticulin and MPL genes. (verywellhealth.com)
20171
  • Wasąg P, Suwińska A, Zakrzewski P , Walczewski J, Lenartowski R, Lenartowska M (2017) Calreticulin localizes to plant intra/extracellular peripheries of highly specialized cells involved in pollen-pistil interactions. (umk.pl)
Isoforms4
  • An autoantibody-mediated immune response to calreticulin isoforms in pancreatic cancer. (nih.gov)
  • Autoantibodies were detected against either one or two calreticulin isoforms identified by mass spectrometry in sera from 21 of 36 patients with pancreatic cancer. (nih.gov)
  • The detection of autoantibodies to calreticulin isoforms may have utility for the early diagnosis of pancreatic cancer. (nih.gov)
  • Diverging functions among calreticulin isoforms in higher plants. (mpg.de)
Immune1
  • 2. Calreticulin as a prognostic biomarker and correlated with immune infiltrate in kidney renal clear cell carcinoma. (nih.gov)
Assays1
  • The interaction of calreticulin with amyloid beta (Aβ) was investigated using solid phase and solution binding assays. (eurekaselect.com)
Macrophages1
  • In addition, SP-D binds to apoptotic cells and stimulates their phagocytosis by macrophages governed by mechanisms dependent and CD91 calreticulin. (biovendor.com)
Antibodies1
  • To investigate the occurrence of anti-calreticulin antibodies (anti-CRT Abs) and evaluate its association with malignancy in patients with idiopathic inflammatory myopathies (IIM). (acrabstracts.org)
Receptor1
  • The results are discussed in the light of a reported role of calreticulin as a cell surface scavenger receptor. (eurekaselect.com)
Calcium homeostasis1
  • 15. Calreticulin is crucial for calcium homeostasis mediated adaptation and survival of thick ascending limb of Henle's loop cells under osmotic stress. (nih.gov)
Induction1
  • Calreticulin requires an ancillary adjuvant for the induction of efficient cytotoxic T cell responses. (helsinki.fi)
Superfamily1
  • Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. (nih.gov)
Affects1
  • It is not clear how the alteration in calreticulin affects the protein's function or leads to the signs and symptoms of essential thrombocythemia. (medlineplus.gov)
Increases1
  • 4. Calreticulin increases growth and progression of natural killer/T-cell lymphoma. (nih.gov)
Concentration2
  • Calreticulin bound Aβ 1-42 in a time and concentration dependent fashion. (eurekaselect.com)
  • It was found that among the tested molecules there is a statistically higher concentration of calreticulin, cathepsin G, CD11b, FcgammaBP and MIP1α in amniotic fluid during intraamniotic infection. (cuni.cz)
Synthetic1
  • Synthetic peptide within Human Calreticulin aa 50-150. (abcam.com)
Store-operated1
  • Here we use mouse embryonic fibroblasts, both wild type (WT) and knockouts (KO) not expressing calreticulin, to investigate the effect of calreticulin on the activation of store-operated Ca entry (SOCE). (qscience.com)
Rabbit1
CALR gene: MedlinePlus Genetics
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Publications | Crick
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Publications | www.ibmc.up.pt
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