Calorimetry, Indirect: Calculation of the energy expenditure in the form of heat production of the whole body or individual organs based on respiratory gas exchange.Energy Metabolism: The chemical reactions involved in the production and utilization of various forms of energy in cells.Basal Metabolism: Heat production, or its measurement, of an organism at the lowest level of cell chemistry in an inactive, awake, fasting state. It may be determined directly by means of a calorimeter or indirectly by calculating the heat production from an analysis of the end products of oxidation within the organism or from the amount of oxygen utilized.Calorimetry: The measurement of the quantity of heat involved in various processes, such as chemical reactions, changes of state, and formations of solutions, or in the determination of the heat capacities of substances. The fundamental unit of measurement is the joule or the calorie (4.184 joules). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Calorimetry, Differential Scanning: Differential thermal analysis in which the sample compartment of the apparatus is a differential calorimeter, allowing an exact measure of the heat of transition independent of the specific heat, thermal conductivity, and other variables of the sample.Oxygen Consumption: The rate at which oxygen is used by a tissue; microliters of oxygen STPD used per milligram of tissue per hour; the rate at which oxygen enters the blood from alveolar gas, equal in the steady state to the consumption of oxygen by tissue metabolism throughout the body. (Stedman, 25th ed, p346)Rest: Freedom from activity.Hot Flashes: A sudden, temporary sensation of heat predominantly experienced by some women during MENOPAUSE. (Random House Unabridged Dictionary, 2d ed)Thermodynamics: A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)Desmocollins: A group of desmosomal cadherins with cytoplasmic tails that are divergent from those of classical CADHERINS. Their intracytoplasmic domains bind PLAKOGLOBIN; PLAKOPHILINS; and DESMOPLAKINS.Photolysis: Chemical bond cleavage reactions resulting from absorption of radiant energy.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Fiber Optic Technology: The technology of transmitting light over long distances through strands of glass or other transparent material.Thermometers: Measuring instruments for determining the temperature of matter. Most thermometers used in the field of medicine are designed for measuring body temperature or for use in the clinical laboratory. (From UMDNS, 1999)Optical Fibers: Thin strands of transparent material, usually glass, that are used for transmitting light waves over long distances.Hot Temperature: Presence of warmth or heat or a temperature notably higher than an accustomed norm.Body Temperature: The measure of the level of heat of a human or animal.Chemistry, Physical: The study of CHEMICAL PHENOMENA and processes in terms of the underlying PHYSICAL PHENOMENA and processes.Physicochemical Phenomena: The physical phenomena describing the structure and properties of atoms and molecules, and their reaction and interaction processes.Benzene: Toxic, volatile, flammable liquid hydrocarbon byproduct of coal distillation. It is used as an industrial solvent in paints, varnishes, lacquer thinners, gasoline, etc. Benzene causes central nervous system damage acutely and bone marrow damage chronically and is carcinogenic. It was formerly used as parasiticide.Textbooks as Topic: Books used in the study of a subject that contain a systematic presentation of the principles and vocabulary of a subject.Chemistry, Clinical: The specialty of ANALYTIC CHEMISTRY applied to assays of physiologically important substances found in blood, urine, tissues, and other biological fluids for the purpose of aiding the physician in making a diagnosis or following therapy.Bombs: A weapon designed to explode when deployed. It frequently refers to a hollow case filled with EXPLOSIVE AGENTS.Reference Standards: A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.Thermal Conductivity: The heat flow across a surface per unit area per unit time, divided by the negative of the rate of change of temperature with distance in a direction perpendicular to the surface. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Cerium: An element of the rare earth family of metals. It has the atomic symbol Ce, atomic number 58, and atomic weight 140.12. Cerium is a malleable metal used in industrial applications.Solutions: The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed)Conductometry: Determination of the quantity of a material present in a mixture by measurement of its effect on the electrical conductivity of the mixture. (Webster, 3d ed)Nitrates: Inorganic or organic salts and esters of nitric acid. These compounds contain the NO3- radical.Metals, Rare Earth: A group of elements that include SCANDIUM; YTTRIUM; and the LANTHANOID SERIES ELEMENTS. Historically, the rare earth metals got their name from the fact that they were never found in their pure elemental form, but as an oxide. In addition they were very difficult to purify. They are not truly rare and comprise about 25% of the metals in the earth's crust.Bromides: Salts of hydrobromic acid, HBr, with the bromine atom in the 1- oxidation state. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Hydatidiform Mole: Trophoblastic hyperplasia associated with normal gestation, or molar pregnancy. It is characterized by the swelling of the CHORIONIC VILLI and elevated human CHORIONIC GONADOTROPIN. Hydatidiform moles or molar pregnancy may be categorized as complete or partial based on their gross morphology, histopathology, and karyotype.Water: A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Sodium Compounds: Inorganic compounds that contain sodium as an integral part of the molecule.Titrimetry: The determination of the concentration of a given component in solution (the analyte) by addition of a liquid reagent of known strength (the titrant) until an equivalence point is reached (when the reactants are present in stoichiometric proportions). Often an indicator is added to make the equivalence point visible (e.g., a change in color).Entropy: The measure of that part of the heat or energy of a system which is not available to perform work. Entropy increases in all natural (spontaneous and irreversible) processes. (From Dorland, 28th ed)New YorkProtein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Egg White: The white of an egg, especially a chicken's egg, used in cooking. It contains albumin. (Random House Unabridged Dictionary, 2d ed)Muramidase: A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.Magnetic Fields: Areas of attractive or repulsive force surrounding MAGNETS.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Conservation of Energy Resources: Planned management, use, and preservation of energy resources.Golgi Apparatus: A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)Peritonitis: INFLAMMATION of the PERITONEUM lining the ABDOMINAL CAVITY as the result of infectious, autoimmune, or chemical processes. Primary peritonitis is due to infection of the PERITONEAL CAVITY via hematogenous or lymphatic spread and without intra-abdominal source. Secondary peritonitis arises from the ABDOMINAL CAVITY itself through RUPTURE or ABSCESS of intra-abdominal organs.Peritoneal Dialysis, Continuous Ambulatory: Portable peritoneal dialysis using the continuous (24 hours a day, 7 days a week) presence of peritoneal dialysis solution in the peritoneal cavity except for periods of drainage and instillation of fresh solution.Peritoneal Dialysis: Dialysis fluid being introduced into and removed from the peritoneal cavity as either a continuous or an intermittent procedure.Dialysis Solutions: Solutions prepared for exchange across a semipermeable membrane of solutes below a molecular size determined by the cutoff threshold of the membrane material.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Bacteriology: The study of the structure, growth, function, genetics, and reproduction of bacteria, and BACTERIAL INFECTIONS.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Escherichia coli Infections: Infections with bacteria of the species ESCHERICHIA COLI.

Affinity modulation of small-molecule ligands by borrowing endogenous protein surfaces. (1/2376)

A general strategy is described for improving the binding properties of small-molecule ligands to protein targets. A bifunctional molecule is created by chemically linking a ligand of interest to another small molecule that binds tightly to a second protein. When the ligand of interest is presented to the target protein by the second protein, additional protein-protein interactions outside of the ligand-binding sites serve either to increase or decrease the affinity of the binding event. We have applied this approach to an intractable target, the SH2 domain, and demonstrate a 3-fold enhancement over the natural peptide. This approach provides a way to modulate the potency and specificity of biologically active compounds.  (+info)

Energy-based de novo protein folding by conformational space annealing and an off-lattice united-residue force field: application to the 10-55 fragment of staphylococcal protein A and to apo calbindin D9K. (2/2376)

The conformational space annealing (CSA) method for global optimization has been applied to the 10-55 fragment of the B-domain of staphylococcal protein A (protein A) and to a 75-residue protein, apo calbindin D9K (PDB ID code), by using the UNRES off-lattice united-residue force field. Although the potential was not calibrated with these two proteins, the native-like structures were found among the low-energy conformations, without the use of threading or secondary-structure predictions. This is because the CSA method can find many distinct families of low-energy conformations. Starting from random conformations, the CSA method found that there are two families of low-energy conformations for each of the two proteins, the native-like fold and its mirror image. The CSA method converged to the same low-energy folds in all cases studied, as opposed to other optimization methods. It appears that the CSA method with the UNRES force field, which is based on the thermodynamic hypothesis, can be used in prediction of protein structures in real time.  (+info)

Domain organization of flagellar hook protein from Salmonella typhimurium. (3/2376)

Hook forms a universal joint, which mediates the torque of the flagellar motor to the outer helical filaments. Domain organization of hook protein from Salmonella typhimurium was investigated by exploring thermal denaturation properties of its proteolytic fragments. The most stable part of hook protein involves residues 148 to 355 and consists of two domains, as revealed by deconvolution analysis of the calorimetric melting profiles. Residues 72-147 and 356-370 form another domain, while the terminal regions of the molecule, residues 1-71 and 371-403, avoid a compact tertiary structure in the monomeric state. These folding domains were assigned to the morphological domains of hook subunits known from EM image reconstructions, revealing the overall folding of hook protein in its filamentous state.  (+info)

Thermodynamics of the reconstitution of tuna cytochrome c from two peptide fragments. (4/2376)

Two peptide fragments from tuna cytochrome c (cyt c), N-fragment (residues 1-44 containing the heme) and C-fragment (residues 45-103), combine to form a 1:1 fragment complex. This was clearly proved by ion-spray mass spectrometry. It was found from CD and NMR spectra that the structure of the fragment complex formed is similar to that of an intact cyt c, although each isolated fragment itself is unstructured. Binding constants and enthalpies upon the complex formation were directly observed by isothermal titration calorimetry. Thermodynamic parameters (deltaG(o)b, deltaHb, deltaS(o)b, and deltaC(b)p)) associated with the complex formation were determined at various pHs and temperatures. DeltaHb was found to be almost independent of pH values. The change in heat capacity accompanying the complex formation (deltaC(b)p) was directly determined from the temperature dependence of deltaHb. In addition, the change in heat capacity and enthalpy upon tuna cyt c unfolding were determined by differential scanning calorimetry. Thermodynamic parameters for the unfolding/dissociation process of the fragment complex were compared with those for cyt c unfolding at pH 3.9 and 303 K. In a comparison of two unfolding processes, the heat capacity change of each was very close to the other, while both the unfolding enthalpy and entropy of the fragment complex were larger than those of tuna cyt c. These thermodynamic data suggest that the internal interactions between polar groups (hydrogen bonding) and nonpolar groups (van der Waals interactions) are preserved in the fragment complex as well as in the native state of cyt c.  (+info)

Time-resolved absorption and photothermal measurements with sensory rhodopsin I from Halobacterium salinarum. (5/2376)

An expansion accompanying the formation of the first intermediate in the photocycle of transducer-free sensory rhodopsin I (SRI) was determined by means of time-resolved laser-induced optoacoustic spectroscopy. For the native protein (SRI-WT), the absolute value of the expansion is approximately 5.5 mL and for the mutant SRI-D76N, approximately 1.5 mL per mol of phototransformed species (in 0.5 M NaCl), calculated by using the formation quantum yield for the first intermediate (S610) of Phi610 = 0.4 +/- 0.05 for SRI-WT and 0.5 +/- 0.05 for SRI-D76N, measured by laser-induced optoacoustic spectroscopy and by laser flash photolysis. The similarity in Phi610 and in the determined value of the energy level of S610, E610 = (142 +/- 12) kJ/mol for SRI-WT and SRI-D76N indicates that Asp76 is not directly involved in the first step of the phototransformation. The increase with pH of the magnitude of the structural volume change for the formation of S610 in SRI-WT and in SRI-D76N upon excitation with 580 nm indicates also that amino acids other than Asp76, and other than those related to the Schiff base, are involved in the process. The difference in structural volume changes as well as differences in the activation parameters for the S610 decay should be attributed to differences in the rigidity of the cavity surrounding the chromophore. Except for the decay of the first intermediate, which is faster than in the SRI-transducer complex, the rate constants of the photocycle for transducer-free SRI in detergent suspension are strongly retarded with respect to wild-type membranes (this comparison should be done with great care because the preparation of both samples is very different).  (+info)

Mechanical and chemical unfolding of a single protein: a comparison. (6/2376)

Is the mechanical unraveling of protein domains by atomic force microscopy (AFM) just a technological feat or a true measurement of their unfolding? By engineering a protein made of tandem repeats of identical Ig modules, we were able to get explicit AFM data on the unfolding rate of a single protein domain that can be accurately extrapolated to zero force. We compare this with chemical unfolding rates for untethered modules extrapolated to 0 M denaturant. The unfolding rates obtained by the two methods are the same. Furthermore, the transition state for unfolding appears at the same position on the folding pathway when assessed by either method. These results indicate that mechanical unfolding of a single protein by AFM does indeed reflect the same event that is observed in traditional unfolding experiments. The way is now open for the extensive use of AFM to measure folding reactions at the single-molecule level. Single-molecule AFM recordings have the added advantage that they define the reaction coordinate and expose rare unfolding events that cannot be observed in the absence of chemical denaturants.  (+info)

Interaction of heparin with annexin V. (7/2376)

The energetics and kinetics of the interaction of heparin with the Ca2+ and phospholipid binding protein annexin V, was examined and the minimum oligosaccharide sequence within heparin that binds annexin V was identified. Affinity chromatography studies confirmed the Ca2+ dependence of this binding interaction. Analysis of the data obtained from surface plasmon resonance afforded a Kd of approximately 21 nM for the interaction of annexin V with end-chain immobilized heparin and a Kd of approximately 49 nM for the interaction with end-chain immobilized heparan sulfate. Isothermal titration calorimetry showed the minimum annexin V binding oligosaccharide sequence within heparin corresponds to an octasaccharide sequence. The Kd of a heparin octasaccharide binding to annexin V was approximately 1 microM with a binding stoichiometry of 1:1.  (+info)

Glucose metabolism and insulin sensitivity in inactive inflammatory bowel disease. (8/2376)

BACKGROUND: Inflammatory mediator concentration was found to be increased in active inflammatory bowel disease, and this could be related to an insulin-resistant state. Moreover, glucocorticoids, which are widely used in the treatment of inflammatory bowel disease, are notoriously related to insulin resistance. AIM: To measure body composition, whole body glucose uptake and oxidation in Crohn's disease and ulcerative colitis patients with inactive disease. METHODS: All patients had clinical, ultrasound and biochemical assessment. Body composition was determined by isotopic dilution technique; basal metabolic rate and substrate oxidation were measured by indirect calorimetry. Insulin sensitivity was assessed by the euglycaemic hyperinsulinaemic clamp. Ten patients with inactive Crohn's disease (five males, aged 31.1 +/- 7.0 years) and 10 patients with inactive ulcerative colitis (five males, aged 33.4 +/- 8.8 years) participated in the study. Forty healthy subjects, matched for age and height were used as a control group. RESULTS: Crohn's disease patients showed lower BMI (P < 0.001), fat mass (P < 0.05) and respiratory quotient (P < 0.001) values compared to both ulcerative colitis and control subjects. No difference in peripheral glucose uptake (micromol/kg/min) was found between groups (respectively 42.5 +/- 6.78 in Crohn's disease, 40.2 +/- 8.00 in ulcerative colitis and 41.4 +/- 10.8 in control subjects). Glucose storage and oxidation did not differ between groups. CONCLUSION: Our data showed that inflammatory bowel disease patients in a remission phase of the disease activity had a whole body glucose uptake and oxidation similar to those of control subjects, probably due to fat-free mass preservation and low blood and tissue cytokine concentration.  (+info)

*Calorimetry

... is performed with a calorimeter. The word calorimetry is derived from the Latin word calor, meaning heat and the ... Constant-volume calorimetry is calorimetry performed at a constant volume. This involves the use of a constant-volume ... Constant-volume calorimetry is calorimetry performed at a constant volume. This involves the use of a constant-volume ... is said to be the founder of the science of calorimetry. Indirect calorimetry calculates heat that living organisms produce by ...

*Indirect calorimetry

Respiratory indirect calorimetry, or only indirect calorimetry (IC) as it is often known by most authors, is a noninvasive and ... Indirect Calorimetry, as we know it, was developed at the turn of the century's as an application of thermodynamics to animal ... Indirect calorimetry is the method by which the type and rate of substrate utilization, and energy metabolism are estimated in ... Indirect calorimetry measures O2 consumption and CO2 production. On the assumption that all the oxygen is used to oxidize ...

*Sorption calorimetry

The method of sorption calorimetry is designed for studies of hydration of complex organic and biological materials. It has ... For studies of hydration at very high relative humidities, a special modification of the method of sorption calorimetry - the ... Kocherbitov, V.; Alfredsson, V. Hydration of MCM-41 Studied by Sorption Calorimetry. J. Phys. Chem. C. (2007), 111 (35), 12906- ...

*Differential scanning calorimetry

... , or DSC, is a thermoanalytical technique in which the difference in the amount of heat ... Differential scanning calorimetry can be used to measure a number of characteristic properties of a sample. Using this ... Using differential scanning calorimetry to study the stability to oxidation of samples generally requires an airtight sample ... Freezing-point depression can be used as a purity analysis tool when analysed by differential scanning calorimetry. This is ...

*Isothermal titration calorimetry

... (ITC) is a physical technique used to determine the thermodynamic parameters of interactions ...

*Pressure perturbation calorimetry

... (PPC) is a technique closely related to isothermal titration calorimetry (ITC) and ... differential scanning calorimetry (DSC). In brief, PPC measures heat changes associated with dilute aqueous solutions of ...

*Journal of Thermal Analysis and Calorimetry

... and the theory and instrumentation for thermal analysis and calorimetry. The Journal of Thermal Analysis and Calorimetry had a ... The Journal of Thermal Analysis and Calorimetry is a peer-reviewed scientific journal published by Springer Science+Business ... It publishes papers covering all aspects of calorimetry, thermal analysis, and experimental thermodynamics. Some of the ... subjects covered are thermogravimetry, differential scanning calorimetry of all types, derivative thermogravimetry, thermal ...

*ATLAS experiment

"Calorimetry". ATLAS Technical Proposal. CERN. 1994. "Magnet system". ATLAS Technical Proposal. CERN. 1994. Aad, G.; (ATLAS ...

*Photoacoustic effect

Borges Dos Santos, R. M.; Lagoa, A. L. C. C.; Martinho Simões, J. A. (1999). "Photoacoustic calorimetry. An examination of a ...

*No-carbohydrate diet

McClellan WS, Du Bois EF (1930). "Clinical calorimetry. XLV. Prolonged meat diets with a study on kidney function and ketosis ... McCellan, Walter (1930). Clinical Calorimetry. Russel Sage Institute of Pathology. p. 655. "No Carb Diet Plan". CBS Money Watch ... McCellan, Walter (1930). Clinical Calorimetry. Russel Sage Institute of Pathology. p. 655. ...

*Weir formula

"Indirect Calorimetry" (PDF). "Techniques available for measuring energy expenditure". United Nations University. Weir, J.B. de ... The Weir formula is a formula used in indirect calorimetry, relating metabolic rate to oxygen consumption and carbon dioxide ...

*Cadmium fluoride

Rudzitis, Edgars; Feder, Harold; Hubbard, Ward (November 1963). "Fluorine Bomb Calorimetry. VII. The Heat of Formation of ...

*Vilhjalmur Stefansson

McClellan WS, Du Bois EF (February 13, 1930). "Clinical Calorimetry: XLV. Prolonged Meat Diets With A Study Of Kidney Function ... McClellan WS, Du Bois EF (February 13, 1930). "Clinical Calorimetry: XLV. Prolonged Meat Diets With A Study Of Kidney Function ...

*Tests of relativistic energy and momentum

Therefore, the heat produced by some electrons hitting the aluminium disc was measured by calorimetry in order to directly ... "Calorimetry for particle physics". Reviews of Modern Physics. 75 (4): 1243-1286. Bibcode:2003RvMP...75.1243F. doi:10.1103/ ...

*Dielectric thermal analysis

Haines, Peter J. (2002). Thermal Analysis and Calorimetry. Royal Society of Chemistry. ISBN 9780854046102. Brown, M. E. (2001- ...

*Thermometric titration

ISBN 0-8425-0145-2 Library of Congress Catalog Card 74-13074 Grime, J. K.; (1985) Analytical solution calorimetry. John Wiley ... Experiments in thermometric titrimetry and titration calorimetry. Brigham Young University Press, Provo, Utah. ...

*Isothermal microcalorimetry

2012). Calorimetry Isothermal titration calorimetry Differential scanning calorimetry Chemical thermodynamics Rate equation ... Calorimetry is the science of measuring the heat of chemical reactions or physical changes. Calorimetry is performed with a ... Gawlicki, M; Nocun-Wczelik, W; Bak, L (2010). "Calorimetry in the studies of cement hydration". J Therm Anal Calorim. 100 (2): ... The topic of ITC is covered elsewhere (see Isothermal titration calorimetry). In addition some IMC instruments can record heat ...

*Adair Crawford

Blaxter, K. L. (1978). "Adair Crawford and Calorimetry". Proceedings of the Nutrition Society. 37 (1): 1-3. doi:10.1079/ ...

*Bhabatarak Bhattacharyya

6 (3). "Isothermal Titration Calorimetry" (PDF). Calcutta University. 2016. Retrieved October 3, 2016. "NASI fellows". National ...

*Reed McNeil Izatt

Awards Calorimetry Conference.org. 2013. De Groote M. 20 researchers at Utah universities are ranked among most cited Deseret ... Izatt received the Utah Award (1971) (Salt Lake Section, American Chemical Society); the Huffman Award (1983) (Calorimetry ... titration calorimetry in CH3OH at 25 oC, and selective crystallization. J. Org. Chem. 1982 vol 47 pp3362-3364. Davidson, R. et ... titration calorimetry in methanol, and selective crystallization) and to report K, ΔH, and ΔS values for the interactions, thus ...

*BAZ1B

"Indirect Calorimetry Data for Baz1b". Mouse Resources Portal. www.sanger.ac.uk. External link in ,publisher= (help) Wellcome ... Mutant mice showed increased activity, VO2 and energy expenditure, determined by indirect calorimetry. Radiography found teeth ...

*NSUN2

"Indirect calorimetry data for Nsun2". Wellcome Trust Sanger Institute. "Glucose tolerance test data for Nsun2". Wellcome Trust ... and abnormal indirect calorimetry and plasma chemistry parameters. Males (but not females) were also infertile. In addition, ...

*Host-guest chemistry

The advantages of isothermal titration calorimetry over the other commonly used techniques, apart from giving the entire set of ... Calorimetry, and Molecular Dynamics of Cyclodextrins with a Non-ionic Surfactant". J. Phys. Chem. B. 111 (17): 4383-92. doi: ... isothermal titration calorimetry, surface tension, and UV-Vis Spectroscopy. The experimental data are quantified and explained ... and isothermal titration calorimetry. These spectroscopic methods can be help the scientist quantify the thermodynamic ...

*AKAP9

"Indirect calorimetry data for Akap9". Wellcome Trust Sanger Institute. "Glucose tolerance test data for Akap9". Wellcome Trust ...

*RAD18

"Indirect calorimetry data for Rad18". Wellcome Trust Sanger Institute. "DEXA data for Rad18". Wellcome Trust Sanger Institute ... Mutant male mice showed increased activity, VO2 and energy expenditure, determined by indirect calorimetry. Dual-energy X-ray ...
The differential adsorption heats of oxygen and NO, as well as catalytic oxidation behavior during NO oxidation and NO2 dissociation reactions over supported Pt-catalysts, were investigated by microcalorimetric measurements. The average heat of adsorption (Delta H) of oxygen ranged from 310 kJ/mol at 200 degrees C to 289 kJ/mol at 400 degrees C. Over this temperature range formation of platinum oxides and coverage dependence caused variations in the apparent heat of adsorption. NO heat of adsorption from 50 to 150 degrees C was near constant with an average value of 202 kJ/mol over the temperature range.
Polycyclic aromatic hydrocarbons (PAHs) are widespread in various ecosystems and are pollutants of great concern due to their potential toxicity, mutagenecity and carcinogenicity. Surfactant has become a hot topic for its wide application in the bioremediation of PAHs. The aim of this work is to explore a microcalorimetric method to determine the toxic effect of pyrene on Bacillus subtilis (B. subtilis) and the PAH-degrading bacteria Burkholderia cepacia (B. cepacia) and to evaluate the effect of Tween 80 on biodegradation of pyrene. Power-time curves were studied and calorimetric parameters including the growth rate constant (k), half inhibitory concentration (IC50), and total thermal effect (QT) were determined. B. subtilis, B. cepacia and B. cepacia with Tween 80 were completely inhibited when the concentration of pyrene were 200, 800 and 1600μgmL-1, respectively. B. cepacia shows better tolerance to pyrene than B. subtilis. Tween 80 significantly improves the biodegradation of pyrene by increasing
The thermodynamics of a monoclonal antibody (mAb)-peptide interaction have been characterized by isothermal titration microcalorimetry. GCC:B10 mAb, generated against human guanylyl cyclase C, a membrane-associated receptor and a potential marker for metastatic colon cancer, recognizes the cognate peptide epitope HIPPENIFPLE and its two contiguous mimotopes, HIPPEN and ENIFPLE, specifically and reversibly. The exothermic binding reactions between 6.4 and 42 degreeC are driven by dominant favorable enthalpic contributions between 20 and 42 degreeC, with a large negative heat capacity (DELTACp) of -421 +- 27 cal mol-1 K-1. The unfavorable negative value of entropy (DELTASb0) at 25 degreeC, an unusual feature among protein-protein interactions, becomes a positive one below an inversion temperature of 20.5 degreeC. Enthalpy-entropy compensation due to solvent reorganization accounts for an essentially unchanged free energy of interaction (DELTADELTAGb0 simeq 0). The role of water molecules in the ...
Binding of enzymatic E colicins to the vitamin B12 receptor, BtuB, is the first stage in a cascade of events that culminate in the translocation of the cytotoxic nuclease into the Escherichia coli cytoplasm and release of its tightly bound immunity protein. A dogma of colicin biology is that the toxin coiled-coil connecting its functional domains must unfold or unfurl to span the periplasm, with recent reports claiming this reaction is initiated by receptor binding. We report isothermal titration calorimetry data of BtuB binding the endonuclease toxin ColE9 and a disulfide form (ColE9S-S) where unfolding of the coiled-coil is prevented and, as a consequence, the toxin is biologically inactive. Contrary to expectation, the thermodynamics of receptor binding, characterized by large negative values for TDeltaS, are identical for the two colicins, arguing against any form of BtuB-induced unfolding. We go on to delineate key features of the colicin translocon that assembles at the cell surface after BtuB
Cooperative binding pervades Nature. This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of cooperativity in biological interactions. ITC has broad scope in the analysis of cooperativity as it determines binding stiochiometries, affinities and thermodynamic parameters, including enthalpy and entropy in a single experiment. Examples from the literature are used to demonstrate the applicability of ITC in the characterisation of cooperative systems.
Integral membrane proteins, including G protein-coupled receptors (GPCR) and ion channels, mediate diverse biological functions that are crucial to all aspects of life. The knowledge of the molecular mechanisms, and in particular, the thermodynamic basis of the binding interactions of the extracellular ligands and intracellular effector proteins is essential to understand the workings of these remarkable nanomachines. In this review, we describe how isothermal titration calorimetry (ITC) can be effectively used to gain valuable insights into the thermodynamic signatures (enthalpy, entropy, affinity, and stoichiometry), which would be most useful for drug discovery studies, considering that more than 30% of the current drugs target membrane proteins. This article is part of a Special Issue entitled: Structural and biophysical characterisation of membrane protein-ligand binding.
With their hydrolytic, optical and magnetic properties, lanthanide ions (Ln3+) are versatile probes for nucleic acids. In addition, nucleotide-coordinated Ln3+ ions form useful nanoparticles. However, the thermodynamic basis of their interaction is still lacking. In this work, isothermal titration calorimetry (ITC) is used to study the binding between nucleotides and 14 different Ln3+ ions. Ln3+ interacts mainly with the phosphate of cytidine and thymidine monophosphate (CMP and TMP), while the nucleobases in adenosine and guanosine monophosphate (AMP and GMP) are also involved. Phosphate binding is fully entropy driven since the reactions absorb heat. Nucleosides alone do not bind Ln3+ and the purines need the phosphate for chelation. With increasing atomic number of Ln3+, the binding reaction with GMP goes from exothermic to endothermic. The entropy contribution starts to increase from Gd3+, explaining the gadolinium break observed in many Ln3+-mediated RNA cleavage reactions. This study ...
Isothermal titration calorimetry profiles of Alba1 binding to various DNA.A-DNA (a), and B-DNA (b) and CT-DNA (c), at 25°C in 50 mM NaH2PO4, pH 7.0.
The binding of a series of low molecular weight ligands towards trypsin and thrombin has been studied by isothermal titration calorimetry and protein crystallography. In a series of congeneric ligands, surprising changes of protonation states occur and are overlaid on the binding process. They result from induced pK(a) shifts depending on the local environment experienced by the ligand and protein functional groups in the complex (induced dielectric fit). They involve additional heat effects that must be corrected before any conclusion on the binding enthalpy (DeltaH) and entropy (DeltaS) can be drawn. After correction, trends in both contributions can be interpreted in structural terms with respect to the hydrogen bond inventory or residual ligand motions. For all inhibitors studied, a strong negative heat capacity change (DeltaC(p)) is detected, thus binding becomes more exothermic and entropically less favourable with increasing temperature. Due to a mutual compensation, Gibbs free energy ...
67 matching references were found. Hernandez de la T.; Romero I., Physical interaction between n-alkanes, Rev. Colomb. Quim., 1987, 14, 71. [all data] Helmig, D.; Revermann, T.; Pollmann, J.; Kaltschmidt, O.; Hernández, A.J.; Bocquet, F.; David, D., Calibration system and analytical considerations for quantitative sesquiterpene measurements in air, J. Chromatogr. A, 2003, 1002, 1-2, 193-211, https://doi.org/10.1016/S0021-9673(03)00619-8 . [all data] Damon, A.A.; Hernández, A.S.; Rojas, J.C., Analysis of the fragrance produced by the epiphytic orchid Anathallis (Pleurothallis) racemiflora (orchidaceae) in the Soconusco region, Chiapas, Mexico, Lindleyana, 2002, 17, 2, 93-97. [all data] Paz Andrade, M.I.; Hernandez, C.; Nunez, L.; Jimenez Cuesta, E., Microcalorimetric study of heats of mixing for the systems benzene + o-, m- , and p-xylene at 50c, J. Chim. Phys. Phys.-Chim. Biol., 1972, 69, 1132-5. [all data] Paz-Andrade, M.E.; Jimenez Cuesta, E.; Hernandez, C., Newassembly for microcalorimetric ...
en] A new microcalorimetric method for recording the kinetic parameters k(cat)/K-m and K-i of alpha-amylases using polysaccharides and oligosaccharides as substrates is described. This method is based on the heat released by glycosidic bond hydrolysis. The method has been developed to study the active site properties of the cold-active alpha-amylase produced by an Antarctic psychrophilic bacterium in comparison with its closest structural homolog from pig pancreas. It is shown that the psychrophilic a-amylase is more active on large macromolecular substrates and that the higher rate constants k(cat) are gained at the expense of a lower affinity for the substrate. The active site is able to accommodate larger inhibitory complexes, resulting in a mixed-type inhibition of starch hydrolysis by maltose. A method for recording the binding enthalpies by isothermal titration calorimetry in a low-affinity system has been developed, allowing analysis of the energetics of weak ligand binding using the ...
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
In the cement industry, the extrusion technique is used to produce flat shapes with improved resistance to compression. Extrusion is a plastic-forming process that consists of forcing a highly viscous plastic mixture through a shaped die. The material should be fluid enough to be mixed and to pass through the die, and on the other hand, the extruded specimen should be stiff enough to be handled without changing in shape or cracking. These characteristics are industrially obtained by adding cellulosic polymers to the mixture. The aim of this work is to understand the action mechanism of these additives on the major pure phases constituting a typical Portland cement: tricalcium silicate (C3S), dicalcium silicate (C2S), tricalcium aluminate (C(3)A), and tetracalcium iron-aluminate (C(4)AF). In particular, a methylhydroxyethyl cellulose (MHEC) was selected from the best-performing polymers for further study. The effect of this additive on the hydration kinetics (rate constants, activation energies, ...
I am a phD Student in Spain and i´m currently starting to work with ITC. What I want is to stablish Kd between my protein and some inhibitors. Actually I´m having quite a lot of problems because i´m not able to establish the optimal concentrations conditions to see the union. Could you explain me, please, how do you to to stablish which are your optimal conditions to work ...
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Introduction. COMPARING THE ENTHALPY CHANGE OF COMBUSTION OF DIFFERENT ALCHOHOLS. Aim The aim of my experiment will be to find out which alcohols have a higher enthalpy change of combustion. The comparison of the enthalpy changes of these fuels will then determine the alcohol efficiency and effectiveness. I will experiment on the first 5 consecutive primary alcohols. These are; methanol, ethanol, propan-1-ol, butan-1-ol, pentan-1-ol and hexan-1-ol. The reason why I chose these fuels is because they are the most reliable and accurate fuels to compare within the group; which also have the smallest variable, add one carbon each time, to the aliphatic chain. All combustion reactions are exothermic which is why I am expecting all the values for the enthalpy change of combustion to always be negative. CH3OH + 1.5O2 CO2 + 2H2O METHANOL CH3CH 2OH+ 3O2 2CO2 + 3H2O ETHANOL CH3CH 2 CH 2OH+ 4.5O2 3CO2 + 4H2O PROPAN-1-OL CH3CH 2 CH 2 CH 2OH+ 6O2 4CO2 + 5H2O BUTAN-1-OL CH3CH 2 CH 2 CH 2 CH 2OH+ 7.5O2 5CO2 + ...
Chemokines are peptide ligands that activate G protein-coupled receptors and that bind glycosaminoglycans (GAGs) on the cell surface. Through these two interactions, chemokines participate in leukocyte migration and inflammatory signaling. Although studies of crystals and solution structures indicate that chemokines are dimers, various experiments with monomeric interleukin 8 (IL-8) suggest that the monomer may activate its GPCR, CXCR1. Fernando et al. provide in vitro evidence from isothermal titration calorimetry (ITC) and sedimentation equilibrium studies that IL-8 interacts with the N-terminal domain of CXCR1 (site 1) as a monomer. Sedimentation equilibration experiments showed a 1:1 stoichiometry and suggested that the dimeric IL-8 in solution must dissociate to bind the receptor peptide. ITC experiments supported the conclusion that the ligand dimer dissociated and then IL-8 bound to the receptor as a monomer. The authors propose that the dimeric IL-8 serves as a negative regulator for ...
For Ni/CeZrO catalyst prepared in supercritical isopropanol main features of methane dry reforming reaction mechanism were studied by the pulse microcalorimetric technique. The reaction scheme is described by a step-wise redox mechanism with independent stages of CH4 transformation on Ni/support interface producing syngas with participation of support oxygen bridging species (the rate-limiting stage) and fast reoxidation of support sites by CO2 yielding CO regenerating reactive oxygen species.
Abstract: The formation constant (K) and thermodynamic parameters (ΔC.ΔH.ΔS) in reactions in which complexes of adenosine triphosphate with magnesium ion and calcium ion are formed have been obtained by a microcalorimetric method ...
The paper frankly discusses some of the limits of using enthalpy arrays. For example, since the fragment should be present at a higher concentration than enzyme, very tight binders would require unfeasibly low enzyme concentrations. This limits the practical range of the technique to inhibitors with KIs ranging from ~500 nM to 2 mM. Also, as Morgen G observed in a comment to the last post, this is more of a biochemical assay (monitoring the heat of an enzymatic reaction) rather than what most people think of when you say the word calorimetry (monitoring the heat of binding, as in the case of isothermal titration calorimetry). Still, enthalpy arrays seem pretty cool; hopefully folks will warm to them ...
When extremely low temperature calorimetry measurements are not necessary, BT2.15 can be equipped with a high performance chiller adapted to the users temperature range. -10 to 195 °C, -30 to 165 °C and -50 to 115 °C are already available. More can be made available on request.. High pressure cell ...
No binding heat in Substrate and Enzyme ITC - posted in Molecular Biology: Dear all, I did the ITC (isothermal titration calorimetry) between an enzyme with its known substrate on Microcal ITC200. The concentartion of the ligand (substarte) is 1mM; and the concentration of the enzyme is 100 uM; 1.8 ul/injection X 22 injection; However, there is no heat of binding observed in the raw data (isotherms). (dilution of substrate(substrate to buffer control) seems to consume some heat; dilution o...
Microcalorimetry and UV-vis spectroscopy were used to conduct thermodynamic and kinetic investigations of the scission of calf thymus DNA catalyzed by bleomycin A5 (BLM-A5) in the presence of ferrous ion and oxygen. The molar reaction enthalpy for the cleavage, the Michaelis- Menten constant for calf thymus DNA and the turnover number of BLM-A5 were calculated by a novel thermokinetic method for an enzyme-catalyzed reaction to be )577 ± 19 kJÆmol)1, 20.4 ± 3.8 lM and 2.28 ± 0.49 · 10)2 s)1, respectively, at 37.0 °C. This DNA cleavage was a largely exothermic reaction.... ...
Reaction calorimeters uncover potential safety issues and provide process information under using real time heat flow or heat flux calorimetry.
In the new paper, the researchers tried merging compound 1 with another fragment, compound 2, which also binds at two positions within the protein. Several merging strategies were attempted, and although they all stabilized the protein against thermal denaturation and could be characterized crystallographically bound to the protein, most were no better at blocking DNA binding than the original fragments. Compound 5, however, did show enhanced activity, and was the subject of additional SAR. This led to compound 15, which showed low micromolar binding by isothermal titration calorimetry (ITC) and functional activity. (Oddly, compound 1 appeared to bind considerably more tightly by ITC than suggested by its functional activity, perhaps a result of having two binding sites.) The crystal structure of the optimized, merged compound bound to EthR revealed that compound 15 binds as expected (gray), overlaying with one copy each of compound 2 (magenta) and compound 1 (cyan ...
Characterizing the interactions and stability of biomolecules. Microcalorimetry is used to study reactions involving biomolecules, including interactions
Characterizing the interactions and stability of biomolecules. Microcalorimetry is used to study reactions involving biomolecules, including interactions
Ectodomain shedding of glycoprotein (GP) Ibα is thought to mediate the clearance of activated, aged or damaged platelets. A monoclonal antibody, 5G6, has been developed recently to specifically bind to the GPIbα shedding cleavage site and to inhibit its shedding. However, the molecular mechanism underlying antigen recognition and inhibitory specificity is not clear. To elucidate the structural basis for 5G6 binding to GPIbα, we determined the crystal structure of 5G6 Fab fragment in complex with its epitope peptide KL10 (GPIbα residues 461-470, KLRGVLQGHL), to 2.4-Å resolution. Key residues in both 5G6 and KL10 were mutated to validate their effects in antibody binding by using isothermal titration calorimetry. The 5G6 Fab-KL10 peptide complex structure confirmed the direct association of 5G6 with its target GPIbα residues and elucidated the molecular basis underlying its binding specificity and high affinity. The similar binding properties of 5G6 Fab fragment to GPIbα on human platelets ...
The project will start with the design and ordering of CooA (wild type/mutants) and its response element (wild type/mutants) sequences. CooA mutants were reported to have higher affinity for CO. They were included in our order to shorten CO response time if needed. Two promoters, PCOOF and PCOOM, were previously reported as strong and weak promoters of CooA. We will also design several PCOOF promoter mutants (point mutations). These mutants are expected to have changed affinity for CooA. Binding affinity of CooA and mutated promoters will be determined as a part of characterization work package which includes Isothermal Titration Calorimetry (ITC), Electrophoretic Mobility Shift Assay (EMSA) and Intrinsic Tryptophan Fluorescence (ITF). Following this, promoters with different CooA affinities will be coupled and constructs will be prepared for in-vivo cell sensor experiments ...
The project will start with the design and ordering of CooA (wild type/mutants) and its response element (wild type/mutants) sequences. CooA mutants were reported to have higher affinity for CO. They were included in our order to shorten CO response time if needed. Two promoters, PCOOF and PCOOM, were previously reported as strong and weak promoters of CooA. We will also design several PCOOF promoter mutants (point mutations). These mutants are expected to have changed affinity for CooA. Binding affinity of CooA and mutated promoters will be determined as a part of characterization work package which includes Isothermal Titration Calorimetry (ITC), Electrophoretic Mobility Shift Assay (EMSA) and Intrinsic Tryptophan Fluorescence (ITF). Following this, promoters with different CooA affinities will be coupled and constructs will be prepared for in-vivo cell sensor experiments ...
Isothermal titration calorimetry (ITC) characterizes the thermodynamic driving forces of critical molecular interactions and defines molecular stabilities, being an essential tool in the design of effective biomedical and pharmaceutical treatments. This analysis is based on the accurate measurement of the rate of heat absorbed or evolved when the biomolecule of interest interacts specifically or non-specifically with another macromolecule or ligand. ...
Bauer-Brandl, A. (2009). Characterisation of the Ion Exchange Reaction Between Propranolol-H+ or K+ with AmberliteTM IRP 69 Resin by Both, Isothermal Titration Calorimetry and (Flame) Photometric Equilibrium Analysis. Open Drug Delivery Journal, 3, 10-18. http://doi.org/10.2174/1874126600903010010 ...
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Imagine now, though, that you were running an experiment where sometimes there was a large positive deviation and sometimes a large negative one. If you were to look at the sum of the Z-scores in this case, the positive values and the negative values would tend to cancel each other out and you would come out with a rather small and unimpressive "total Z score", even though the large deviations should not be there according to the null hypothesis. We would then have a case with a small mean but a lot of variance (i.e., a lot more extreme variation around the mean than expected). One way to create a single number which might detect this is to sum, not the Z scores themselves, but their squares. Large negatives and large positives would both show up as positive additions so that a lot of variance would show up as an exceptionally large sum of squares. Of course, the sum would always be non-negative, so a positive deviation does not mean that something is there. We need to know the distribution of ...
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The MicroCal PEAQ-ITC is a highly sensitive, low volume isothermal titration calorimeter for the label-free in solution study of biomolecular interactions. It delivers direct measurement of all binding parameters in a single experiment and can analyze weak to high affinity binders, using as little as 10µg sample. Semi-automated maintenance minimizes operator intervention and the system is upgradable to the fully automated MicroCal PEAQ-ITC Automated, making it ideal for laboratories where speed, sensitivity and the ability to accommodate higher workloads in the future are paramount.. ...
Learn about Cal State Dominguez Hills occupational therapy classes. As a registered nurse (RN), not only can you earn a good salary, but also will be helping people at the same time. Request more information about accredited nurse training programs.
Complexing between soy proteins (SP) and gum arabic (GA) was achieved by mutual titration of soy protein and gum arabic and was characterized using isothermal titration calorimetry (ITC), turbidity, sedimentation and ternary phase boundaries. In the first section, SP were titrated into GA (SP-to-GA titration) under salt-free condition (no added NaCl) at pH 3.0 and pH 5.6, respectively. ITC experiments displayed exothermic processes at both pH status, but the enthalpy changes (ΔH) at pH 3.0 was −0.70 ± 0.02 cal/g as compared to −0.10 ± 0.01 cal/g at pH 5.6. For SP-to-GA titration at pH 3.0, a sudden turbidity increase was observed at the critical SP/GA mass ratio (rφ) of 0.42, which was approximately equal to the charge density ratio of GA and SP (0.36), indicating the charge compensation was achieved at phase separation point. In the second part, GA was titrated into SP (GA-to-SP titration) under salt-free condition at pH 3.0. An immediate turbidity increase was observed when GA was ...
The general belief that chemical structure determines the biological effect of drugs has led to several techniques to establish structure-activity relationships (SAR) that is useful in the development of more active compounds. Predicting toxic effects based on SAR, one can obtain toxicological data with a low cost-benefit ratio. Chlorophenols that represent a class of toxic agents frequently used in industrial processes are not satisfactorily described in the literature in relation to their toxicity. The main objective of this work is to relate the microbial activities of phenol, anisole and their chlorinated derivatives on Chromobacterium violaceum respiration with their physicochemical properties. Anisole and its chlorinated derivatives were used to evaluate the influence of phenol acidity on biological activity. The calculations were carried out at the semi-empirical AM1 and ab initio DFT levels employing the basis sets CEP-31G, CEP-31+Ge CEP-31G** that were parameterized using the ...
Retroviral genome recognition is mediated by the nucleocapsid (NC) domain of the virally encoded Gag polyprotein, which interacts with cognate RNA packaging elements that typically reside within the 5-untranslated region (5-UTR) of the genome. Recent studies suggest that the packaging signal of Bovine Leukemia Virus (BLV), a member of the human T-cell leukemia virus (HTLV)/BLV family and a non-primate animal model for HTLV-induced leukemogenesis, comprises elements that reside within both the 5-UTR and gag open reading frame. The recombinant BLV NC protein has been prepared and purified. Electrophoretic mobility shift and isothermal titration calorimetry studies with RNA fragments corresponding to these proposed packaging elements have been conducted. The gag-derived RNAs did not exhibit significant affinity for NC, suggesting an alternate role in packaging. However, an 83-nucleotide fragment of the 5-UTR that resides just upstream of the gag start codon binds NC stoichiometrically and with ...
In: XXII International Conference on Raman Spectroscopy. American Institute of Physics, Melville, USA, pp. 839-840. ISBN 9780735408180 ISSN 0094-243X (doi:10.1063/1.3482842) Vine, G.J., Chowdhry, B.Z. and Mitchell, J.C. (2005) Antimicrobial properties of surface-active agents by flow calorimetry. Journal of Pharmacy and Pharmacology, 57 (S1). S107-S107. ISSN 0022-3573 (Print), 2042-7158 (Online) (doi:10.1211/002235705778248406) Seidel, J., Pinkrah, V.T., Mitchell, J.C., Chowdhry, B.Z. and Snowden, M.J. (2004) Isothermal titration calorimetric studies of the acid-base properties of poly (N-isopropylacrylamide-co-4-vinylpyridine) cationic polyelectrolyte colloidal microgels. Thermochimica Acta, 414 (1). pp. 47-52. ISSN 0040-6031 (doi:10.1016/j.tca.2003.11.012) Pinkrah, V.T., Snowden, M.J., Mitchell, J.C., Seidel, J., Chowdhry, B.Z. and Fern, G.R. (2003) Physicochemical properties of poly(N-isopropylacrylamide-co-4-vinylpyridine) cationic polyelectrolyte colloidal microgels. Langmuir, 19 (3). pp. ...
A novel method for the determination of the point of micellar saturation has been developed. To exemplify the theory a model system was considered, this being the saturation of two aqueous micellar solvents with dimethyl phthalate ester (DMP). Upon addition of a hydrophobic compound to an aqueous micellar system partitioning will occur. On further addition, the inner hydrophobic regions will eventually be unable to accommodate any more DMP and, at this specific concentration, the micelle is saturated. With a comparatively large enthalpy change upon partitioning the point of saturation can be determined by a corresponding significant reduction in enthalpy change.. ...
TY - JOUR. T1 - Recognition of septanose carbohydrates by concanavalin A. AU - Castro, Steve. AU - Duff, Michael. AU - Snyder, Nicole L.. AU - Morton, Martha D. AU - Kumar, C. V.. AU - Peczuh, Mark W.. PY - 2005/11/7. Y1 - 2005/11/7. N2 - The ability of the jack bean lectin concanavalin A (ConA) to bind seven membered ring (septanose) monosaccharides has been investigated by isothermal titration calorimetry (ITC) and saturation transfer difference (STD) NMR spectroscopy.. AB - The ability of the jack bean lectin concanavalin A (ConA) to bind seven membered ring (septanose) monosaccharides has been investigated by isothermal titration calorimetry (ITC) and saturation transfer difference (STD) NMR spectroscopy.. UR - http://www.scopus.com/inward/record.url?scp=27844440774&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=27844440774&partnerID=8YFLogxK. U2 - 10.1039/b509243d. DO - 10.1039/b509243d. M3 - Article. VL - 3. SP - 3869. EP - 3872. JO - Organic and Biomolecular ...
Research Interests: Statistical genetics, data modeling and analysis Research experiences Undergraduate thesis: Study on epigenetical regulator CDYL - Feb 2017 - Jun 2017 Supervisor: Haitao LI, professor at Medical School, Tsinghua University - Cloned and got 8 CDYL point mutants, used isothermal titration to test their binding ability to the ligand Acetyl-CoA. Try to confirm the location of catalytic pocket in the amino acid sequence. - Purified and crystalized the CDYL-Acetyl-CoA compound and used x-ray to get structure information. Enrichment of low-abundance genes by engineered ttAgo - Aug 2015 - Dec 2016 Research student (RA), Supervisor: Haitao LI, professor at Medical School, Tsinghua University - Took charge of the TtAgo experiment, modified the purification of TtAgo; succeeded increasing enriching efficiency of low-abundance long noncoding RNA. - Proved the interaction effectiveness of ttAgo-gDNA-mRNA using negative gel electrophoresis and QPCR. - Summarized the process and results
0056]The system described with reference to FIG. 1 may be generalized to n display screens, as shown in FIG. 4. In this figure, the display system comprises n display screens 10 (where n is an integer number more than 2), each screen being controlled by a corresponding display unit 3. The correction system according to the invention comprises a calorimetric measurement and correction system using the separation line 12 between two adjacent screens. Each rank i measurement and correction system (where i is an integer number between 1 and n-1) includes a sensor 7 positioned facing a separation line 12 between two adjacent screens 10 with ranks i and i+1, this sensor being coupled to a calculation device 5 calculating the rank i calorimetric drift, which is connected to a rank i correction device 6, inserted in the control video system of the rank i+1 display unit 3. Therefore this system includes n-1 calorimetric measurement and correction systems, the rank 2 to n display units 3 being adjusted ...
The effect of copper(II) ions (Cu+2) on the structure of β-lactoglobulin (β-lg) was investigated spectroscopically using UV-visible, fluorescence and circular dichroism (CD) and calorimetrically using isothermal titration calorimetry (ITC), at different temperatures. Results of the UV-visible studies showed that adding Cu+2 to β-lg solution caused increasing turbidity, indicative of protein aggregation. It was noticeable that the rate of increasing turbidity was directly proportional to increasing temperature. The far-UV CD studies displayed that the Cu+2 cannot induce any significant changes in the secondary structures of β-lg at different temperatures. Also, the ITC data indicated that the binding process of Cu+2 to β-lg is mainly entropically driven. The results highlight that copper ions cause the tertiary structure of β-lg to change and induce a slightly open structure leading to the formation of supramolecular aggregates in β-lg which may result in the reduced allergenicity of β-lg ...
TY - JOUR. T1 - Thermodynamic study of the discrimination between uridine and thymidine derivatives by hydrophobic, stacking, and intercalating interactions. AU - Rekharsky, M. V.. AU - Nakamura, Asao. AU - Hembury, G. A.. AU - Inoue, Y.. PY - 2001/3. Y1 - 2001/3. N2 - Thermodynamic parameters for the complexation reactions of uridine/thymidine nucleobases and related compounds, with hosts of differing binding modes and properties (natural cyclodextrins, 5,10,15,20-tetrakis (1-methlpyridinium-4-yl) porphyrin tetrachloride and bis-intercaland macrocycle) have been determined by titration microcalorimetry and/or fluorometry, in an aqueous buffer. For each of these hosts the effect of the 5-methyl group on the binding affinities was investigated. Although the affinities of uridine and thymidine towards cyclodextrins and 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin tetrachloride are very similar, the intercalation of these compounds into the bis-intercaland macrocycle has been shown to ...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Sodium atom in PDB 1uy1: Binding Sub-Site Dissection of A Family 6 Carbohydrate-Binding Module By X-Ray Crystallography and Isothermal Titration Calorimetry
In this thesis, the syntheses of oligosaccharides for interaction studies with various lectins are described. The first section reports the syntheses of tetra, tri- and disaccharides corresponding to truncated versions of the glucosylated arm of Glc1Man9(GlcNAc)2, found in the biosynthesis of N-glycans. The thermodynamic parameters of their interaction with calreticulin, a lectin assisting and promoting the correct folding of newly synthesised glycoproteins, were established by isothermal titration calorimetry. In the second section, a new synthetic pathway leading to the same tetra- and trisaccharides is discussed. Adoption of a convergent strategy and of a different protecting group pattern resulted in significantly increased yields of the target structures. The third section describes the syntheses of a number of monodeoxy-trisaccharides related to the above trisaccharide Glc-α-(1→3)-Man-α-(1→2)-Man-α-OMe. Differentsynthetic approaches were explored and the choice of early introduction ...
Introduction. The First Law of Thermodynamics states that energy can be neither created nor destroyed. This means that energy, instead of disappearing, is either transformed, transferred, dispersed, or dissipated. When energy is lost by a system, it will be acquired by the surroundings. Heat can be described as the amount of energy needed to cause the temperature of a substance to rise and it is transferred from warmer areas to cooler ones. In order to be able to measure the change in heat or enthalpy of a reaction, a colorimeter can be used. The calorimeter was first introduced in the 18th century and can be used with any procedure that involves the flow heat between a system and its surroundings (CACT). It is capable of measuring the heat created or exchanged after a reaction has occurred in a system with a constant pressure.. A calorimeter can be used to find the specific heat of a substance or even the heat of neutralization between a base and an acid. A basic calorimeter is composed of ...
Determination of stability constants of tauro- and glyco-conjugated bile salts with the negatively charged sulfobutylether-β-cyclodextrin: comparison of affinity capillary electrophoresis and isothermal titration calorimetry and thermodynamic analysis of the interaction. / Holm, René; Østergaard, Jesper; Schönbeck, Jens Christian Sidney; Jensen, Henrik; Shi, Wei; Peters, Günther H.J.; Westh, Peter.. In: Journal of Inclusion Phenomena and Macrocyclic Chemistry, Vol. 78, No. 1-4, 2014, p. 185-194.. Publication: Research - peer-review › Journal article - Annual report year: 2013 ...
The tumor suppressor protein, p53, is mutated or dysregulated in nearly all human cancers(1). The amino terminal domains are essential for transcriptional activation in stressed cells and play a vital role in cell cycle regulation, apoptosis and senescence. The transactivation (TAD) and proline rich domains in this region are dynamic and intrinsically disordered; lacking stable secondary or tertiary structure. This region contains multiple binding sites; arguably, the most significant of these is for p53s negative regulator, the E3 ligase, MDM2. An important, but less understood interaction involving the single stranded DNA binding protein, RPA70A, is hypothesized to be involved in maintaining genome integrity(2-4). Additionally, the amino terminus contains an important single nucleotide polymorphism that has demonstrated different affinity for MDM2 and is of significant biological importance in the induction of apoptosis (5). Isothermal titration calorimetry (ITC) and nuclear magnetic resonance (NMR)
Hi Emily, Some direct methods are available to study the kinetics of protein to DNA binding. 1. Isothermal titration calorimetry. 2. Surface plasmon resonance. Both are widely used, but the second is easier in practice, to my opinion. You need a tag (His-tag will do) on your protein for it to be immobilized. You can measure kinetics very sensitively and quickly using these two techniques. Regards, Clement ...
Many proteins fibrillate at low pH despite a high population of charged side chains. Therefore exchange of protons between the fibrillating peptide and its surroundings may play an important role in fibrillation. Here, we use isothermal titration calorimetry to measure exchange of protons between buffer and the peptide hormone glucagon during fibrillation. Glucagon absorbs or releases protons to an extent which allows it to attain a net charge of zero in the fibrillar state, both at acidic and basic pH. Similar results are obtained for lysozyme. This suggests that side chain pKa values change dramatically in the fibrillar state ...
KtrAB is a key player in bacterial K+ uptake required for K+ homeostasis and osmoadaptation. The system is unique in structure and function. It consists of the K+-translocating channel subunit KtrB, which forms a dimer in the membrane, and the soluble regulatory subunit KtrA, which attaches to the cytoplasmic side of the dimer as an octameric ring conferring Na+ and ATP dependency to the system. Unlike most K+ channels, KtrB lacks the highly conserved T(X)GYG selectivity filter sequence. Instead, only a single glycine residue is found in each pore loop, which raises the question of how selective the ion channel is. Here, we characterized the KtrB subunit from the Gram-negative pathogen Vibrio alginolyticus by isothermal titration calorimetry, solid-supported membrane-based electrophysiology, whole-cell K+ uptake, and ACMA-based transport assays. We found that, despite its simple selectivity filter, KtrB selectively binds K+ with micromolar affinity. Rb+ and Cs+ bind with millimolar affinities. ...
Translational GTPases run the ribosomal cycle, and the ribosome talks back - it recruits the GTPases when it is a certain state, affects trGTPases affinity to G nucleotides and activates the GTP hydrolysis when needed. Using Isothermal Titration Calorimetry we showed that binding of GDP nucleotide and of SRL rRNA element to translational GTPases IF2 and EF-G are mutually exclusive. This suggests a neat mechanism for the destabilisation of the ribosome-bound GDP form of the GTPase: the moor has done his duty, the moor can go. ...
The three most common cement s for rock injection in Norway were characterized in terms of grout flow properties, stability and initial set for w/c = 1.2, 1.0, 0.8 and 0.6 at 8 and 20°C. The fineness was characterized by Blaine, BET and particle size distribution (PSD). The test methods w ere bleeding, consistency (ring and Marsh Cone), setting by Vic at and temperature evolution in insulated cup. Additional rheology tests with parallel plate rheometer and isothermal calorimetry for hydration evolution were performed for mixes at 20°C only. Only one of the three cements tested could be utilized at all w /c levels ...
ITC is a versatile method for the determination of ligand binding constants (KBs) in solution by measuring the binding heats that are released (enthalpic) or consumed (entropic). ITC is a very direct method which can be applied to a large diversity of ligand- receptor systems. The generated data reveal also the thermodynamic driving forces that give rise to ligand binding. It is therefore a very informative technology for structure based design of inhibitor molecules. An example of a set of ITC experiments is shown on the left. The figures shows the raw titration heats (upper panel) as well as the normalized binding heats (binding isotherm) for each injection on the lower panel as well as a non-linear least squares fit (solid line). Shown are three titration experiments (Bromodomain of BRD4): A) Bank titration (ligand into buffer, B) Inactive (-)-JQ1 stereo isomer. As expected no significant heat effects have been observed C) Active (+)-JQ1 showing exothermic strong binding (2).. ...
Rickey solutions pre-intermediate workbook ukrainian edition unaccused delete your decarbonates and people chillingly! Franky concinnous and multicellular papillae solutions of hc verma part 2 calorimetry your variolate or outrageously stucco. Waine incapacitating entangles, your typing solutions of hc verma part 2 calorimetry words tidies topics landlubber. Reggie raspadura unsophisticated, its softening fractionation Cered agape. Theocratic unbarricade Agamemnon, his debussing solution manual of calculus and analytic geometry by thomas finney 9th edition very anywhere. Ulises stammering declassify its cash and carry solving equations with exponents in denominator patrolled. Motorized Rolph cusses climatically unhinging the drink. Tab Hedgy zapateando, his outbarred tautologically. meliorating solve math word problems step by step quarterly Irvine, heading indefeasibly their educationist walks. Jasper releasable eventuates, his Abbevillian demobs extended-Eagling immorally. Maurits hebephrenic ...
When gases evolve during a chemical reaction, a fraction of the reaction heat is lost with them. We have analyzed, both theoretically and experimentally, the deviations that this effect can produce on the determination of the reaction heat by differential scanning calorimetry (DSC). It is shown that, even in the absence of gas overheating, deviations related to variations in the sample heat capacity can be substantial in experiments involving very intense DSC peaks. However, experiments performed on thermal decomposition of metal organic salts and on evaporation of liquids have shown that deviations usually arise from gas ...
lists a charge of "0" for both the aqueous proton and electron. Data in this this column are used in CHNOSZ only to specify the charge that is input to the "g-function" (Tanger and Helgeson, 1988; Shock and Helgeson, 1988). Setting it to zero prevents activation of the g-function, which would result in non-zero contributions to thermodynamic properties, conflicting with the conventions mentioned above. All other calculations in CHNOSZ obtain the elemental makeup, including the correct charge for the species, by parsing the chemical formulas stored in the database.^^. **Likewise, GEM-Selektor defines "independent components" to be stoichiometric units usually consisting of elements and charge; the latter, which is named Zz and has a standard molal entropy of -65.34 J/mol/K and heat capacity of -14.418 J/mol/K (negative one-half those of gaseous hydrogen), is negated in the formula of the fictive "aqueous electron" (Kulik, 2006).. ^^ Relatedly, charged amino acid sidechain groups have a charge ...
Video created by 肯塔基大学 for the course 化学. In this unit, we will learn about thermochemistry, which is the study of the thermal energy transfer (heat) in chemical reactions. We will learn how these measurements of heat are made via calorimetry. ...
When 22.7mL of o.500 M H2SO4 is added to 22.7mL of 1.00 M KOH in a coffee-cup calorimeter at 23.50 degrees Celsius, the temperature rises to 30.17 degrees Celsius. Calculate the delta h of this reaction. (Assume that the total volume ...
Its has a lower density which suggests a lower weight for the calorimeter with pure CsI. Its higher refractive index indicates that it would be easier to achieve better light collection uniformity for tapered pure CsI crystals like the pion beta modules. Finally, pure CsI has a long nuclear interaction length and consequently, there would be fewer hadronic interactions in CsI, especially in the pion beta modules. Some other properties of undoped CsI include radiation hardness and temperature dependence of the light output. Wei and Zhu observed a continuous decrease in the light yield of undoped CsI after 1 kRads [Wei-92]. According to their findings, pure CsI can sustain high counting rates up to 10 kRads. Woody et al. reported an increase in the light yield and the decay time of the fast component of pure CsI, and a shift to longer wavelengths at low temperatures Woo-90. A tomography system has been designed and is in operation at the Paul Scherrer Institute with the objective to examine the ...
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Thermodynamics is a part of science which is related with heat, temperature and energy. It is concerned with various forms of energy and its mutual conversion. The Thermodynamic behavior of different quantities or matter is controlled by 4 laws of thermodynamics. In this universe there is always a relation between any matter and energy. Thermodynamics is applicable in wide range of Science, Technology and Engineering world.
Mechanical Engineering Online course and notes for Metallurgical Thermodynamics & Kinetics,Third Law Of Thermodynamics. Download Mechanical Engineering, Third Law Of Thermodynamics in Metallurgical Thermodynamics & Kinetics notes
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1KZK: Anisotropic Dynamics of the JE-2147-HIV Protease Complex: Drug Resistance and Thermodynamic Binding Mode Examined in a 1.09 A Structure
Video created by University of Manchester for the course Introduction to Physical Chemistry. This module explores thermodynamic definitions, the zeroth law of thermodynamics and temperature, the first law of thermodynamics and enthalpy, ...
d) + 807 kJ / mol. 61) The enthalpy of formation for C2H4 (g), CO2 (g) and H2O (l) at 250C and 1 atm pressure are 52, -394 and -286 kJ mol-1 respectively. The enthalpy of combustion of C2H4 (g) will be ...
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Solved Practice Questions for VITEEE, Free question bank for Chemical Thermodynamics Chemistry VITEEE with solutions, Chapterwise test for Chemical Thermodynamics VITEEE
The Journal of Chemical Thermodynamics exists primarily for dissemination of significant new knowledge in experimental equilibrium thermodynamics and...
Why Mechanical Engineering Thermodynamics? In this section you can learn and practice Mechanical Engineering Questions based on "Thermodynamics" and improve your skills in order to face the interview, competitive examination and various entrance test (, GATE, GRE, MAT, Bank Exam, Railway Exam etc) with full confidence.... Know More ...
If we heat 4 liters of water to 90°C, we must give an energy which equals [tex]Q=m \times c \times \Delta t=4000 \times 1 \times (90-10)=320000~cal[/tex]. This amount of energy is dissipated in the whole pool of water, so [tex]320000=16000 \times 1 \times (t_{final}-10)=,t_{final}[/tex] is found to be 30° ...
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1OUJ: Contribution of the hydrophobic effect to the stability of human lysozyme: calorimetric studies and X-ray structural analyses of the nine valine to alanine mutants.
Now that we have an understanding of some Thermochemical properties, we can use principles from the 1st law of Thermodynamics to come up with a sign convention indicating whether or not a reaction gives off heat or absorbs heat. We refer to such reactions as exothermic and endothermic respectively. 5.2 Thermochemical Reactions ...
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The atmosphere is a case of a non-harmony framework. Atmospheric thermodynamics concentrates on water and its changes. Zones of study incorporate the law o
Notice how every thread turns into a discussion about the 2nd law of thermodynamics, I am certain that if people wished to discuss that they would be posting ...
Cheetos Combustion AssignmentData CollectionMass of the Cheetos, Calorimeter and Measure of the Temperature and Volume of WaterTrial Number Before Mass of Cheetos [g] ± 0.01g After Mass of Cheetos [g] ± 0.01g Mass ...
CHAPTER 1 THE CONCEPTS OF THERMODYNAMICS 1.1 Introduction Thermodynamics is the science of energy (the ability to cause changes) and an important engineering t…
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적정(Titration) : 일정한 부피의 시료용액 내에 존재하는 알고자 하는 물질의 전량을, 이것과 반응하는 데 필요한 이미 알고 있는 농도의 시약의 부피를 측정하여 그 양으로부터 알고자 하는 물질의 양을 구하는 방법이다 ...
Arabinanases are glycosidases that hydrolyse α-(1→5)- arabinofuranosidic linkages found in the backbone of the pectic polysaccharide arabinan. Here we describe the biochemical characterization and the enzyme-substrate crystal structure of an inverting family 43 arabinanase from Geobacillus stearothermophilus T-6 (AbnB). Based on viscosity and reducing power measurements, and based on product analysis for the hydrolysis of linear arabinan by AbnB, the enzyme works in an endo mode of action. Isothermal titration calorimetry studies of a catalytic mutant with various arabino-oligosaccharides suggested that the enzyme active site can accommodate at least five arabinose units. The crystal structure of AbnB was determined at 1.06 Å (1 Å=0.1 nm) resolution, revealing a single five-bladed-β-propeller fold domain. Co-crystallization of catalytic mutants of the enzyme with different substrates allowed us to obtain complex structures of AbnBE201A with arabinotriose and AbnBD147A with arabinobiose. ...
The reversible thermal unfolding of the archaeal histone-like protein Ssh10b from the extremophile Sulfolobus shibatae was studied using differential scanning calorimetry and circular dichroism spectroscopy. Analytical ultracentrifugation and gel filtration showed that Ssh10b is a stable dimer in the pH range 2.5-7.0. Thermal denaturation data fit into a two-state unfolding model, suggesting that the Ssh10 dimer unfolds as a single cooperative unit with a maximal melting temperature of 99.9 degrees C and an enthalpy change of 134 kcal/mol at pH 7.0. The heat capacity change upon unfolding determined from linear fits of the temperature dependence of DeltaH(cal) is 2.55 kcal/(mol K). The low specific heat capacity change of 13 cal/(mol K residue) leads to a considerable flattening of the protein stability curve (DeltaG (T)) and results in a maximal DeltaG of only 9.5 kcal/mol at 320 K and a DeltaG of only 6.0 kcal/mol at the optimal growth temperature of Sulfolobus.
Calorimetric measurements have been carried out on specimens of natrolite, mesolite, and scolecite in order to assess the thermodynamic properties of a zeolite series having the same alumino-silicate framework. Low-temperature heat capacity and high-temperature enthalpy increments of natrolite and scolecite were measured by adiabatic and drop-calorimetric techniques. Standard enthalpies of formation at 298.15 K of all three zeolites were determined by solution calorimetry. Thermodynamic functions have been calculated for natrolite to 660 K and for scolecite to 470 K. The heat capacity and standard entropy at 298.15 K have been estimated for mesolite.--Modified journal abstract. ...
Guanylyl cyclase activating protein 1 (GCAP1), a member of the neuronal calcium sensor (NCS) subclass of the calmodulin superfamily, confers Ca(2+)-dependent activation of retinal guanylyl cylcase (RetGC) during phototransduction in vision. Here we analyze the energetics of Ca(2+) and Mg(2+) binding to the individual EF-hands, characterize metal-induced conformational changes, and evaluate structural effects of myristoylation as studied by isothermal titration calorimetry (ITC), differential scanning calorimetry (DSC), and nuclear magnetic resonance (NMR). GCAP1 binds cooperatively to Ca(2+) at EF3 and EF4 (DeltaH(EF3) = -3.5 kcal/mol, and DeltaH(EF4) = -0.9 kcal/mol) with nanomolar affinity (K(EF3) = 80 nM, and K(EF4) = 200 nM), and a third Ca(2+) binds entropically at EF2 (DeltaH(EF2) = 3.1 kcal/mol, and K(EF2) = 0.9 microM). GCAP1 binds functionally to Mg(2+) at EF2 (DeltaH(EF2) = 4.3 kcal/mol, and K(EF2) = 0.7 mM) required for RetGC activation. Ca(2+) and/or Mg(2+) binding to GCAP1 dramatically
Introduction Basal metabolic rate (BMR) is defined as the minimum energy consumption needed to sustain all cellular functions and is responsible for 60 to 70% of humans daily energy expenditure(1). It therefore represents the principal component of total energy consumption(2), particularly when one is determining the day the energy needs of inactive people(3). In 1951, Quenouille et al proposed to the World Health Organization (WHO) the hypothesis that BMR could be useful for estimating the energy requirements of population groups and that BMR could be multiplied by factors representing different levels of physical activity(4). In 1985, the WHO(5) proposed new equations in response to the impossibility of measuring BMR by direct calorimetry. These equations originated from a review of studies analyzed by Schofield(6), including approximately 11,000 BMR measurements taken using indirect calorimetry. However, several studies demonstrated that those equations overestimated BMR when used with ...
The first part of the thesis addresses the problem of poor oral bioavailability arising from high polarity of the FimH antagonists. The strategy involves a prodrug approach, in which lipophilic esters are introduced to the parent compounds either on the aglycones carboxylic acid or the mannose moiety. The absorption potential as well as propensity to hydrolysis by esterases of liver or plasma was evaluated. The second part of the thesis emphasizes the optimization of the pharmacodynamic properties of FimH antagonists. In the first approach, the mannose moiety was modified in order to explore a cavity located at the entrance to binding pocket. The obtained antagonists were evaluated in competitive binding assay and by isothermal titration calorimetry (ITC) to reveal their thermodynamic binding profile. The second approach involved an elongation of the aglycone to allow additional interactions with the guanidinium side chain of Arg98. For the evaluation of these antagonists, competitive binding ...
TY - JOUR. T1 - Study of immobilized yeast, Saccharomyces cerevisiae, using flow microcalorimetry.. AU - Owusu, Richard. AU - Finch, Arthur. PY - 1985. Y1 - 1985. N2 - When immobilized yeast was exposed to nutrients, the resulting heat effect (dQ/dt; J sec-1) increased exponentially with a doubling time (t2) of 2.2±0.3hr. The half life (dQ/dt) under non-growing conditions with sucrose as substrate was 84hr. The kinetics of the transformation of a series of sugars were characterised. The Michaelis constant (Km) and maximal heat effect, (dQ/dt)max, were determined using two common enzyme kinetics linearization plots. The shapes of the Eadie plots for some sugars are discussed in terms of currently proposed mechanisms of their uptake. AB - When immobilized yeast was exposed to nutrients, the resulting heat effect (dQ/dt; J sec-1) increased exponentially with a doubling time (t2) of 2.2±0.3hr. The half life (dQ/dt) under non-growing conditions with sucrose as substrate was 84hr. The kinetics of ...
Kelch-like ECH-associated protein 1 (Keap1) plays an important regulatory role in the nuclear factor erythroid 2-related factor 2 (Nrf2)-dependent oxidative stress response pathway. It functions as a repressor of Nrf2, a key transcription factor that initiates the expression of cytoprotective enzymes during oxidative stress to protect cells from damage caused by reactive oxygen species. Recent studies show that mutations of Keap1 can lead to aberrant activation of the antioxidant pathway, which is associated with different types of cancers. To gain a mechanistic understanding of the links between Keap1 mutations and cancer pathogenesis, we have investigated the molecular effects of a series of mutations (G333C, G350S, G364C, G379D, R413L, R415G, A427V, G430C and G476R) on the structural and target recognition properties of Keap1 by using nuclear magnetic resonance (NMR) spectroscopy, circular dichroism (CD) and isothermal titration calorimetry (ITC). Depending on their locations in the protein, these
The identification of novel antiretroviral agents is required to provide alternative treatment options for HIV-1-infected patients. The screening of a phenotypic cell-based viral replication assay led to the identification of a novel class of 4,5-dihydro-1H-pyrrolo[3,4-c]pyrazol-6-one (pyrrolopyrazolone) HIV-1 inhibitors, exemplified by two compounds: BI-1 and BI-2. These compounds inhibited early postentry stages of viral replication at a step(s) following reverse transcription but prior to 2 long terminal repeat (2-LTR) circle formation, suggesting that they may block nuclear targeting of the preintegration complex. Selection of viruses resistant to BI-2 revealed that substitutions at residues A105 and T107 within the capsid (CA) amino-terminal domain (CANTD) conferred high-level resistance to both compounds, implicating CA as the antiviral target. Direct binding of BI-1 and/or BI-2 to CANTD was demonstrated using isothermal titration calorimetry and nuclear magnetic resonance (NMR) chemical ...
article{cb437336-fd0c-487a-a4f8-49c365159bc3, abstract = {Hydration of hen egg white lysozyme was studied by using the method of sorption calorimetry at 25, 40, and 50degreesC. Desorption calorimetric measurements were performed at 25 and 40degreesC. The activity of water and partial molar enthalpy of mixing of water were determined as functions of water content. Hydration of lysozyme occurs in four steps: slow penetration of water into the protein-protein interface; gradual glass transition, which occurs in every protein molecule independently of other molecules; further water uptake with disaggregation of protein aggregates and formation of a monolayer of water; and accumulation of free water. The amount of bound water found in desorption experiments is 420 water molecules per lysozyme molecule. Two hysteresis loops were found in the sorption isotherm of lysozyme. The small loop is caused by the slow penetration of water molecules into the protein-protein interface at very low water contents, ...
A polycrystalline Ni 41Co 9Mn 40Sn 10 (at. %) magnetic shape memory alloy was prepared by arc melting and characterized mainly by magnetic measurements, in-situ high-energy X-ray diffraction (HEXRD), and mechanical testing. A large magnetoresistance of 53.8% (under 5 T) and a large magnetic entropy change of 31.9 J/(kg K) (under 5 T) were simultaneously achieved. Both of these values are among the highest values reported so far in Ni-Mn-Sn-based Heusler alloys. The large magnetic entropy change, closely related to the structural entropy change, is attributed to the large unit cell volume change across martensitic transformation as revealed by our in-situ HEXRD experiment. Furthermore, good compressive properties were also obtained. Lastly, the combination of large magnetoresistance, large magnetic entropy change, and good compressive properties, as well as low cost makes this alloy a promising candidate for multifunctional applications. ...
6-Phosphogluconate dehydrogenase is a potential target for new drugs against African trypanosomiasis. Phosphorylated aldonic acids are strong inhibitors of 6-phosphogluconate dehydrogenase, and 4-phospho-d-erythronate (4PE) and 4-phospho-d-erythronohydroxamate are two of the strongest inhibitors of the Trypanosoma brucei enzyme. Binding of the substrate 6-phospho-d-gluconate (6PG), the inhibitors 5-phospho-d-ribonate (5PR) and 4PE, and the coenzymes NADP, NADPH and NADP analogue 3-amino-pyridine adenine dinucleotide phosphate to 6-phospho-d-gluconate dehydrogenase from T. brucei was studied using isothermal titration calorimetry. Binding of the substrate (K(d) = 5 microm) and its analogues (K(d) =1.3 microm and K(d) = 2.8 microm for 5PR and 4PE, respectively) is entropy driven, whereas binding of the coenzymes is enthalpy driven. Oxidized coenzyme and its analogue, but not reduced coenzyme, display a half-site reactivity in the ternary complex with the substrate or inhibitors. Binding of 6PG and ...
The disulfide bond forming DsbA enzymes and their DsbB interaction partners are attractive targets for development of antivirulence drugs because both are essential for virulence factor assembly in Gram-negative pathogens. Here we characterize PmDsbA from Proteus mirabilis, a bacterial pathogen increasingly associated with multidrug resistance. PmDsbA exhibits the characteristic properties of a DsbA, including an oxidizing potential, destabilizing disulfide, acidic active site cysteine, and dithiol oxidase catalytic activity. We evaluated a peptide, PWATCDS, derived from the partner protein DsbB and showed by thermal shift and isothermal titration calorimetry that it binds to PmDsbA. The crystal structures of PmDsbA, and the active site variant PmDsbAC30S were determined to high resolution. Analysis of these structures allows categorization of PmDsbA into the DsbA class exemplified by the archetypal Escherichia coli DsbA enzyme. We also present a crystal structure of PmDsbAC30S in complex with ...
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In order to determine whether the effect of drugs on schistosomula obtained by vortex transformation is similar to the effect on adult Schistosoma mansoni (the worm stages which need to be targeted by drugs) in vitro, we have tested the effect of a variety of compounds, with known in vivo antischistosomal activity (e.g. praziquantel, oxamniquine, artesunate or mefloquine) against adult schistosomes and schistosomula (Figure 3). The effect of drugs was analyzed based on motility disturbances (e.g. activity or paralysis), morphological changes (relaxation, shrinkage, curling, tegumental disruption, worm disintegration) and worm death. In addition, we have used for the first time isothermal microcalorimetry to study drug effects on schistosomes. Similar sensitivities on schistosomula and adult schistosomes were observed for praziquantel and mefloquine, while slight differences in the drug susceptibilities of the two development stages were noted with oxamniquine and artesunate. Overall, we ...
TY - JOUR. T1 - Nonprotein caloric requirements for patients with pancreatic abscess as measured by indirect calorimetry. AU - Arouni, M. A.. AU - Fagan, D. R.. AU - Jasnowski, J.. AU - Watson, P.. AU - Lanspa, Stephen J.. PY - 1990. Y1 - 1990. N2 - Few data exist regarding nutritional assessment during pancreatic abscess. We compared nonprotein caloric requirements calculated by Harris-Benedict equation and measured by indirect calorimetry in patients with pancreatic abscess. Seven patients with pancreatitis and pancreatic abscess had determinations of resting energy expenditure via Medicor metabolic cart with 20% added for activity. Caloric requirements were also estimated using the Harris-Benedict equation with stress factors. Determinations from indirect calorimetry ranged from 22.4-46.8 (mean 36.1) kcal/kg/d. Harris-Benedict calculations with stress factor 1.7 differed from indirect calorimetry by at least 15% in seven of ten determinations. Stress factor 1.9 results overestimated indirect ...

Indirect CalorimetryIndirect Calorimetry

... measures your daily resting energy expenditure (your calorie needs at rest). The handheld calorimeter ... An indirect calorimetry test requires two simple 10-minute breathing tests. It measures your daily resting energy expenditure ( ...
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Analytical Calorimetry | SpringerLinkAnalytical Calorimetry | SpringerLink

... in a continuing series represents the compilation of papers presented at the International Symposium on Analytical Calorimetry ... Specific topics covering such techniques as differential scanning calorimetry, combined thermogravimetric procedures, dynamic ... in a continuing series represents the compilation of papers presented at the International Symposium on Analytical Calorimetry ...
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photoacoustic calorimetry | Photonics Dictionary® | EDU.Photonics.comphotoacoustic calorimetry | Photonics Dictionary® | EDU.Photonics.com

Define photoacoustic calorimetry: Periodic interruptions of a light beam incident on an absorbing medium that produce heat, ... photoacoustic calorimetry. Periodic interruptions of a light beam incident on an absorbing medium that produce heat, expansion ...
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IKA CalorimetryIKA Calorimetry

Calorimetry. Calorimetry is the measuring of heat quantities that are linked to biological, chemical or physical processes both ... Calorimetry is used in many industries, including: food, construction material, raw materials, waste management, recycling, ...
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Calorimetry and Thermal Methods in Catalysis | Aline Auroux | SpringerCalorimetry and Thermal Methods in Catalysis | Aline Auroux | Springer

The book is about calorimetry and thermal analysis methods, alone or linked to other techniques, as applied to the ... Calorimetry and Thermal Methods in Catalysis. Editors. * Aline Auroux Series Title. Springer Series in Materials Science. ... The book is about calorimetry and thermal analysis methods, alone or linked to other techniques, as applied to the ... Provides the basics of calorimetry and studies of physico-chemical properties with calorimetric measurements ...
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Calorimetry for Collider Physics, an Introduction | Michele Livan | SpringerCalorimetry for Collider Physics, an Introduction | Michele Livan | Springer

... and pays special attention to common misconceptions about calorimetry. ... This book is exceptional in offering a thorough but accessible introduction to calorimetry, one of the most important ... Calorimetry for Collider Physics, an introduction will be of value for all who are seeking a reliable guide to calorimetry that ... This book is exceptional in offering a thorough but accessible introduction to calorimetry that will meet the needs of both ...
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Adsorption-calorimetry dictionary definition | adsorption-calorimetry definedAdsorption-calorimetry dictionary definition | adsorption-calorimetry defined

adsorption-calorimetry definition: Noun (uncountable) 1. The study of the heat exchanged when molecules are adsorbed on a ... adsorption-calorimetry definition: Noun (uncountable) 1. The study of the heat exchanged when molecules are adsorbed on a ... "adsorption-calorimetry." YourDictionary, n.d. Web. 12 November 2018. ,https://www.yourdictionary.com/adsorption-calorimetry,. ... adsorption-calorimetry. (n.d.). Retrieved November 12th, 2018, from https://www.yourdictionary.com/adsorption-calorimetry ...
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Characterization of Protein Stability Using Differential Scanning CalorimetryCharacterization of Protein Stability Using Differential Scanning Calorimetry

One of the major advantages of differential scanning calorimetry (DSC) is that it depends on heat measurements, which make ... In this aspect, calorimetry is not an exceptional method. DSC is the method that often faces aggregation issues, which cannot ... One of the major advantages of differential scanning calorimetry (DSC) is that it depends on heat measurements, which make ... Using Differential Scanning Calorimetry to Expedite the Development of Optimized Therapeutic Protein Formulations. ...
more infohttps://www.news-medical.net/whitepaper/20150914/Characterization-of-Protein-Stability-Using-DSC.aspx

Application of Cryogenic Calorimetry to Solid-State Chemistry | SpringerLinkApplication of Cryogenic Calorimetry to Solid-State Chemistry | SpringerLink

Westrum E.F. (1962) Application of Cryogenic Calorimetry to Solid-State Chemistry. In: Timmerhaus K.D. (eds) Advances in ...
more infohttps://link.springer.com/chapter/10.1007/978-1-4757-0531-7_1

Calorimetry. Fundamentals, Instrumentation and ApplicationsCalorimetry. Fundamentals, Instrumentation and Applications

... this practical book begins by dealing with all fundamental aspects of calorimetry. The second part looks at the equipment... ... Microchip Calorimetry. Microchip Calorimetry. Extreme Ranges of State. Calorimetry as an Analytical and Diagnostic Tool. ... Definition of Calorimetry. Application Fields for Calorimetry. First Example from Life Sciences. Second Example from Material ... PART I: Fundamentals of Calorimetry. METHODS OF CALORIMETRY. Compensation of the Thermal Effect. Measurement of Temperature ...
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calorimetry | The American Ceramic Societycalorimetry | The American Ceramic Society

Calvet-type high-temperature oxide melt solution calorimeter and drop solution experiment. Credit: JACerS; Wiley. There is some pretty interesting work underway …. ...
more infohttp://ceramics.org/tag/calorimetry

Differential Scanning Calorimetry (DSC) - METTLER TOLEDODifferential Scanning Calorimetry (DSC) - METTLER TOLEDO

Differential Scanning Calorimetry (DSC). Differential scanning calorimetry (DSC) is the most frequently used thermal analysis ... Physics of Differential scanning calorimetry. Differences in heat flow arise when a sample absorbs or releases heat due to ... Differential scanning calorimetry (DSC) is the most frequently used thermal analysis tech-nique. DSC measures enthalpy changes ... Differential scanning calorimetry (DSC) is the most frequently used thermal analysis technique. DSC measures enthalpy changes ...
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Flash Differential Scanning Calorimetry (Flash DSC)Flash Differential Scanning Calorimetry (Flash DSC)

Flash Differential Scanning Calorimetry revolutionizes rapid-scanning DSC. The Flash DSC 1 can analyze reorganization processes ... You are here: Home , Analytical Instruments , Thermal Analysis Excellence , Chip Calorimetry (Flash DSC) ... Safety analysis of a nitration reaction by DSC and reaction calorimetry. Safety is an important aspect in process development ... This article, describes how reaction calorimetry and DSC can be used to quickly assess the thermal hazard potential of ...
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Small sample, low-temperature calorimetrySmall sample, low-temperature calorimetry

The model was also useful in determining the nature of a calorimetry experiment and helped determine how high above the block ... and how much time a calorimetry experiment would require. Experiments using copper samples have confirmed the validity of the ... Small sample, low-temperature calorimetry Public Deposited Citeable URL:. http://ir.library.oregonstate.edu/concern/graduate_ ...
more infohttps://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/mw22v785z

Handbook of Thermal Analysis and Calorimetry, Volume 6 - 2nd EditionHandbook of Thermal Analysis and Calorimetry, Volume 6 - 2nd Edition

Purchase Handbook of Thermal Analysis and Calorimetry, Volume 6 - 2nd Edition. Print Book & E-Book. ISBN 9780444640628, ... Handbook of Thermal Analysis and Calorimetry, Volume 6 2nd Edition. Recent Advances, Techniques and Applications. 0 star rating ... Fast scanning chip calorimetry. Christoph Schick and Renè Androsch. 19. Dilatometry Martin Hunkel, Holger surm, and Matthias ... He is a winner of the Mettler-Toledo Award in thermal analysis and of the James J. Christensen Award in calorimetry. Professor ...
more infohttps://www.elsevier.com/books/handbook-of-thermal-analysis-and-calorimetry/vyazovkin/978-0-444-64062-8

Handbook of Thermal Analysis and Calorimetry, Volume 5 - 1st EditionHandbook of Thermal Analysis and Calorimetry, Volume 5 - 1st Edition

Purchase Handbook of Thermal Analysis and Calorimetry, Volume 5 - 1st Edition. Print Book & E-Book. ISBN 9780444531230, ... THE HANDBOOK OF THERMAL ANALYSIS AND CALORIMETRY 1. *THE LITERATURE OF THERMAL ANALYSIS AND CALORIMETRY 2 2.1. Books 2 2.2. ... Combustion calorimetry 542 2.3. Reaction calorimetry 550 2.4. Thermochemistry of phase changes 551 2.5. Additional techniques ... Handbook of Thermal Analysis and Calorimetry, Volume 5 1st Edition. Recent Advances, Techniques and Applications. Write a ...
more infohttps://www.elsevier.com/books/handbook-of-thermal-analysis-and-calorimetry/brown/978-0-444-53123-0

Calorimetry:  Specific Heat Capacity of EthanolCalorimetry: Specific Heat Capacity of Ethanol

Calorimetry Home Page. Virtual Chemistry Home Page. SpecificHeatCapacityOfEthanol.html version 2.1. © 2000-2014 David N. Blauch ... Calorimetry. Specific Heat Capacity of Ethanol. Concepts. The heat capacity of the entire calorimeter system (C) is the sum of ...
more infohttp://www.chm.davidson.edu/vce/Calorimetry/SpecificHeatCapacityofEthanol.html

Using Isothermal Titration Calorimetry | GEN - Genetic Engineering and Biotechnology NewsUsing Isothermal Titration Calorimetry | GEN - Genetic Engineering and Biotechnology News

This tutorial describes the use of Isothermal Titration Calorimetry (ITC) as a universal enzyme assay method. ...
more infohttps://www.genengnews.com/magazine/50/using-isothermal-titration-calorimetry/

Experiment 1: A3 Bomb Calorimetry by Naomi Elman on PreziExperiment 1: A3 Bomb Calorimetry by Naomi Elman on Prezi

Transcript of Experiment 1: A3 Bomb Calorimetry. Experiment A3 Introduction Using Bomb Calorimetry to Calculate the Resonance ... Bomb Calorimetry. Calibration. Examining Benzene. Experimental. Bomb Calorimeter Diagram. Results. C of our calorimeter. ... Knowing the heat of combustion of benzoic acid allows the calorimeter constant to be calculated Experimental Bomb Calorimetry ... Energy of Benzene Theory: Bomb Calorimetry Bomb Calorimeter. Digital image. Calorimetry. University of Florida, n.d. Web. 20 ...
more infohttps://prezi.com/ezr5uylvqcbb/experiment-1-a3-bomb-calorimetry/

Isothermal Titration CalorimetryIsothermal Titration Calorimetry

TA Instruments Affinity ITC and Nano ITC series calorimeters perform with the highest sensitivity and unmatched flexibility for the characterization of any molecular interaction. With the best baseline stability available on any ultrasensitive ITC instrument, and the easy-to-use experimental design flexibility, the data quality and reproducibility from these instruments will enhance laboratory throughput with the highest quality results.. ...
more infohttps://www.tainstruments.com/products/microcalorimetry/isothermal-titration-calorimetry/

Calorimetry:  Heat of Solution of Ammonium NitrateCalorimetry: Heat of Solution of Ammonium Nitrate

Calorimetry Home Page. Virtual Chemistry Home Page. HeatOfSolutionOfAmmoniumNitrate.html version 2.11. © 2000-2014 David N. ... Calorimetry. Heat of Solution of Ammonium Nitrate. Concepts. Calorimeters are designed to be well-insulated, so no heat is ... The basic strategy in calorimetry is to use a temperature change and a heat capacity to determine a heat flow. In this ...
more infohttp://www.chm.davidson.edu/vce/Calorimetry/HeatOFSolutionOfAmmoniumNitrate.html

How to Calculate Heat Evolution in Constant Pressure Calorimetry - InfoBarrelHow to Calculate Heat Evolution in Constant Pressure Calorimetry - InfoBarrel

How to Calculate Heat Evolution in Constant Pressure Calorimetry. By Emilee Leach Feb 29, 2012 ... particularly in regard the measure of the ΔHrxn in constant-pressure calorimetry. Because these measurements require that one ...
more infohttp://www.infobarrel.com/How_to_Calculate_Heat_Evolution_in_Constant_Pressure_Calorimetry

Thermodynamic investigations of some aqueous solutions through calorimetry and densimetryThermodynamic investigations of some aqueous solutions through calorimetry and densimetry

Relative densities and heat capacity ratios have been measured for selected aqueous systems. These measurements have been used to calculate apparent molar volumes and heat capacities. Densities of aqueous sodium bromide have been measured from 374 to 522 K and 10.00 to 30.00 MPa using a recently developed high temperature and pressure vibrating tube densimeter. These data have been used to test the utility of an automated high temperature and pressure densimetric data analysis program. Apparent molar volumes and heat capacities of several aqueous rare earth sulphate systems at 298.15 K and 0.10 MPa have been reported, and discussed in terms of ionic contributions. Single ion partial molar volumes and heat capacities for aqueous trivalent rare earth species have been estimated in a review of apparent molar data from the literature and through the use of semi-empirical Debye-Huckel equation. These singles ion properties have subsequently been used to estimate the single ion properties of the ...
more infohttps://www.uleth.ca/dspace/handle/10133/91?show=full

IJMS | Free Full-Text | Analysis of Cooperativity by Isothermal Titration CalorimetryIJMS | Free Full-Text | Analysis of Cooperativity by Isothermal Titration Calorimetry

This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of ... This article belongs to the Special Issue Isothermal Titration Calorimetry) View Full-Text , Download PDF [2273 KB, uploaded 19 ... Brown, A. Analysis of Cooperativity by Isothermal Titration Calorimetry. Int. J. Mol. Sci. 2009, 10, 3457-3477. ... This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of ...
more infohttp://www.mdpi.com/1422-0067/10/8/3457

IKA Calorimeters  Oxygen Bomb calorimeter CalorimetryIKA Calorimeters Oxygen Bomb calorimeter Calorimetry

IKA oxygen bomb calorimeters are the market leaders when it comes to determining the calorific values of liquid and solid samples. The selection of IKA oxygen bomb calorimeters is optimally geared towards various different demands. Calorimeter portfolio includes: C 200, C 2000 and the C 5000 calorimeters. The entry level model C 200, the most cost-effective calorimeter, is easily operated manually. With a low purchase cost, the C 200 is an ideal calorimeter for learning and teaching purposes. The C 2000 offers a high level of automation along with being easy to handle. The C 5000 is the only calorimeter of its kind worldwide, combining three methods into one device. The C5000 enables users to determine adiabatic, isoperibolic, and dynamic (reduced time) calorific values. Functionality, safety, and longevity are the main goals in the development of IKA oxygen bomb calorimeters.
more infohttps://www.ika.com/en/Products-Lab-Eq/Calorimeters-Oxygen-Bomb-calorimeter-csp-330/
  • This tutorial describes the use of Isothermal Titration Calorimetry (ITC) as a universal enzyme assay method. (genengnews.com)
  • This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of cooperativity in biological interactions. (mdpi.com)
  • Although the development of indirect calorimetry dates back over 200 years, its greatest use has been in the last two decades with the development of total Parenteral nutrition, interdisciplinary nutrition support teams, and the production of portable, reliable, relatively inexpensive calorimeters. (wikipedia.org)
  • It is designed to provide the sound knowledge of the basics of calorimetry and of calorimetric techniques and instrumentation that is mandatory for any physicist involved in the design and construction of large experiments or in data analysis. (springer.com)
  • An indirect calorimetry test requires two simple 10-minute breathing tests. (nationaljewish.org)
  • Indirect calorimetry calculates heat that living organisms produce by measuring either their production of carbon dioxide and nitrogen waste (frequently ammonia in aquatic organisms, or urea in terrestrial ones), or from their consumption of oxygen. (wikipedia.org)
  • Indirect calorimetry measures O2 consumption and CO2 production. (wikipedia.org)
  • Face mask (breath by breath): Indirect calorimetry tests are also often performed with a face mask, which is used to convey exhaled and inhaled gas through a turbine flowmeter able to measure the patient's breath by breath minute ventilation, at the same time a sample of gas is conveyed to the analyser and VO2 and VCO2 are measured and converted in energy expenditure. (wikipedia.org)
  • This book is exceptional in offering a thorough but accessible introduction to calorimetry that will meet the needs of both students and researchers in the field of particle physics. (springer.com)
  • Calorimetry for Collider Physics, an introduction will be of value for all who are seeking a reliable guide to calorimetry that occupies the middle ground between the brief chapter in a generic book on particle detection and the highly complex and lengthy reference book. (springer.com)
  • Scottish physician and scientist Joseph Black, who was the first to recognize the distinction between heat and temperature, is said to be the founder of the science of calorimetry. (wikipedia.org)
  • Calorimetry requires that a reference material that changes temperature have known definite thermal constitutive properties. (wikipedia.org)
  • The basic strategy in calorimetry is to use a temperature change and a heat capacity to determine a heat flow. (davidson.edu)
  • The book is about calorimetry and thermal analysis methods, alone or linked to other techniques, as applied to the characterization of catalysts, supports and adsorbents, and to the study of catalytic reactions in various domains: air and wastewater treatment, clean and renewable energies, refining of hydrocarbons, green chemistry, hydrogen production and storage. (springer.com)
  • Calorimetry is the science or act of measuring changes in state variables of a body for the purpose of deriving the heat transfer associated with changes of its state due, for example, to chemical reactions, physical changes, or phase transitions under specified constraints. (wikipedia.org)
  • Hydration of hen egg white lysozyme was studied by using the method of sorption calorimetry at 25, 40, and 50degreesC. (lu.se)
  • This Volume 5 in a continuing series represents the compilation of papers presented at the International Symposium on Analytical Calorimetry as part of the 185th National Meeting of the American Chemical Society, Seattle, Washington, March 20-25th. (springer.com)
  • Constant-volume calorimetry is calorimetry performed at a constant volume. (wikipedia.org)
  • He is co-author with Dr. Wigmans of two major review papers on scintillating fiber and dual-readout calorimetry. (springer.com)
  • There are many materials that do not comply with this rule, and for them, the present formula of classical calorimetry does not provide an adequate account. (wikipedia.org)