Calmodulin-Binding Proteins: Proteins which bind calmodulin. They are found in many tissues and have a variety of functions including F-actin cross-linking properties, inhibition of cyclic nucleotide phosphodiesterase and calcium and magnesium ATPases.Calmodulin: A heat-stable, low-molecular-weight activator protein found mainly in the brain and heart. The binding of calcium ions to this protein allows this protein to bind to cyclic nucleotide phosphodiesterases and to adenyl cyclase with subsequent activation. Thereby this protein modulates cyclic AMP and cyclic GMP levels.Trifluoperazine: A phenothiazine with actions similar to CHLORPROMAZINE. It is used as an antipsychotic and an antiemetic.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Myosin-Light-Chain Kinase: An enzyme that phosphorylates myosin light chains in the presence of ATP to yield myosin-light chain phosphate and ADP, and requires calcium and CALMODULIN. The 20-kDa light chain is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors. The enzyme plays a central role in the regulation of smooth muscle contraction.Calcium-Binding Proteins: Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.GAP-43 Protein: A nervous tissue specific protein which is highly expressed in NEURONS during development and NERVE REGENERATION. It has been implicated in neurite outgrowth, long-term potentiation, SIGNAL TRANSDUCTION, and NEUROTRANSMITTER release. (From Neurotoxicology 1994;15(1):41-7) It is also a substrate of PROTEIN KINASE C.Kinetics: The rate dynamics in chemical or physical systems.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Myosin Type I: A subclass of myosins found generally associated with actin-rich membrane structures such as filopodia. Members of the myosin type I family are ubiquitously expressed in eukaryotes. The heavy chains of myosin type I lack coiled-coil forming sequences in their tails and therefore do not dimerize.Neurogranin: A BRAIN-specific substrate for PROTEIN KINASE C that binds CALMODULIN and is involved in regulation of CALCIUM SIGNALING.Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Calcium-Transporting ATPases: Cation-transporting proteins that utilize the energy of ATP hydrolysis for the transport of CALCIUM. They differ from CALCIUM CHANNELS which allow calcium to pass through a membrane without the use of energy.Poly(A)-Binding Proteins: Proteins that bind to the 3' polyadenylated region of MRNA. When complexed with RNA the proteins serve an array of functions such as stabilizing the 3' end of RNA, promoting poly(A) synthesis and stimulating mRNA translation.Authorship: The profession of writing. Also the identity of the writer as the creator of a literary production.Knowledge Bases: Collections of facts, assumptions, beliefs, and heuristics that are used in combination with databases to achieve desired results, such as a diagnosis, an interpretation, or a solution to a problem (From McGraw Hill Dictionary of Scientific and Technical Terms, 6th ed).Canrenoic Acid: A synthetic pregnadiene derivative with anti-aldosterone activity.Editorial Policies: The guidelines and policy statements set forth by the editor(s) or editorial board of a publication.Scientific Misconduct: Intentional falsification of scientific data by presentation of fraudulent or incomplete or uncorroborated findings as scientific fact.Electronic Mail: Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.Accounts Payable and Receivable: Short-term debt obligations and assets occurring in the regular course of operational transactions.Health Insurance Portability and Accountability Act: Public Law 104-91 enacted in 1996, was designed to improve the efficiency and effectiveness of the healthcare system, protect health insurance coverage for workers and their families, and to protect individual personal health information.Transient Receptor Potential Channels: A broad group of eukaryotic six-transmembrane cation channels that are classified by sequence homology because their functional involvement with SENSATION is varied. They have only weak voltage sensitivity and ion selectivity. They are named after a DROSOPHILA mutant that displayed transient receptor potentials in response to light. A 25-amino-acid motif containing a TRP box (EWKFAR) just C-terminal to S6 is found in TRPC, TRPV and TRPM subgroups. ANKYRIN repeats are found in TRPC, TRPV & TRPN subgroups. Some are functionally associated with TYROSINE KINASE or TYPE C PHOSPHOLIPASES.Glutathione Transferase: A transferase that catalyzes the addition of aliphatic, aromatic, or heterocyclic FREE RADICALS as well as EPOXIDES and arene oxides to GLUTATHIONE. Addition takes place at the SULFUR. It also catalyzes the reduction of polyol nitrate by glutathione to polyol and nitrite.TRPC Cation Channels: A subgroup of TRP cation channels that contain 3-4 ANKYRIN REPEAT DOMAINS and a conserved C-terminal domain. Members are highly expressed in the CENTRAL NERVOUS SYSTEM. Selectivity for calcium over sodium ranges from 0.5 to 10.Calcium Channels: Voltage-dependent cell membrane glycoproteins selectively permeable to calcium ions. They are categorized as L-, T-, N-, P-, Q-, and R-types based on the activation and inactivation kinetics, ion specificity, and sensitivity to drugs and toxins. The L- and T-types are present throughout the cardiovascular and central nervous systems and the N-, P-, Q-, & R-types are located in neuronal tissue.TRPV Cation Channels: A subgroup of TRP cation channels named after vanilloid receptor. They are very sensitive to TEMPERATURE and hot spicy food and CAPSAICIN. They have the TRP domain and ANKYRIN repeats. Selectivity for CALCIUM over SODIUM ranges from 3 to 100 fold.Flagella: A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)Kinesin: A microtubule-associated mechanical adenosine triphosphatase, that uses the energy of ATP hydrolysis to move organelles along microtubules toward the plus end of the microtubule. The protein is found in squid axoplasm, optic lobes, and in bovine brain. Bovine kinesin is a heterotetramer composed of two heavy (120 kDa) and two light (62 kDa) chains. EC 3.6.1.-.Dyneins: A family of multisubunit cytoskeletal motor proteins that use the energy of ATP hydrolysis to power a variety of cellular functions. Dyneins fall into two major classes based upon structural and functional criteria.Chlamydomonas: A genus GREEN ALGAE in the order VOLVOCIDA. It consists of solitary biflagellated organisms common in fresh water and damp soil.Chlamydomonas reinhardtii: A species of GREEN ALGAE. Delicate, hairlike appendages arise from the flagellar surface in these organisms.Microtubules: Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.Calpain: Cysteine proteinase found in many tissues. Hydrolyzes a variety of endogenous proteins including NEUROPEPTIDES; CYTOSKELETAL PROTEINS; proteins from SMOOTH MUSCLE; CARDIAC MUSCLE; liver; platelets; and erythrocytes. Two subclasses having high and low calcium sensitivity are known. Removes Z-discs and M-lines from myofibrils. Activates phosphorylase kinase and cyclic nucleotide-independent protein kinase. This enzyme was formerly listed as EC 3.4.22.4.Cell Death: The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.Pancreatic Polypeptide: A 36-amino acid pancreatic hormone that is secreted mainly by endocrine cells found at the periphery of the ISLETS OF LANGERHANS and adjacent to cells containing SOMATOSTATIN and GLUCAGON. Pancreatic polypeptide (PP), when administered peripherally, can suppress gastric secretion, gastric emptying, pancreatic enzyme secretion, and appetite. A lack of pancreatic polypeptide (PP) has been associated with OBESITY in rats and mice.Brain Ischemia: Localized reduction of blood flow to brain tissue due to arterial obstruction or systemic hypoperfusion. This frequently occurs in conjunction with brain hypoxia (HYPOXIA, BRAIN). Prolonged ischemia is associated with BRAIN INFARCTION.Neurons: The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.Transducin: A heterotrimeric GTP-binding protein that mediates the light activation signal from photolyzed rhodopsin to cyclic GMP phosphodiesterase and is pivotal in the visual excitation process. Activation of rhodopsin on the outer membrane of rod and cone cells causes GTP to bind to transducin followed by dissociation of the alpha subunit-GTP complex from the beta/gamma subunits of transducin. The alpha subunit-GTP complex activates the cyclic GMP phosphodiesterase which catalyzes the hydrolysis of cyclic GMP to 5'-GMP. This leads to closure of the sodium and calcium channels and therefore hyperpolarization of the rod cells. EC 3.6.1.-.GTP-Binding Proteins: Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.Chromatography, Affinity: A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Guanosine 5'-O-(3-Thiotriphosphate): Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.Computer Security: Protective measures against unauthorized access to or interference with computer operating systems, telecommunications, or data structures, especially the modification, deletion, destruction, or release of data in computers. It includes methods of forestalling interference by computer viruses or so-called computer hackers aiming to compromise stored data.Skin: The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.Confidentiality: The privacy of information and its protection against unauthorized disclosure.Privacy: The state of being free from intrusion or disturbance in one's private life or affairs. (Random House Unabridged Dictionary, 2d ed, 1993)Portal Vein: A short thick vein formed by union of the superior mesenteric vein and the splenic vein.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Cations: Positively charged atoms, radicals or groups of atoms which travel to the cathode or negative pole during electrolysis.

Adducin polymorphism affects renal proximal tubule reabsorption in hypertension. (1/1442)

Abnormalities in renal sodium reabsorption may be involved in the development and maintenance of experimental and clinical hypertension. Adducin polymorphism is thought to regulate ion transport in the renal tubule. It has recently been shown that there is a significant linkage of alpha-adducin locus to essential hypertension and that the 460Trp allele is associated with hypertension. Patients with this allele display larger blood pressure changes with body sodium variation. The aim of this study was to test whether alpha-adducin polymorphism is involved in abnormalities of renal function. Because proximal tubular reabsorption has been shown to be tightly coupled to renal perfusion pressure, this segmental tubular function was investigated in 54 (29 Gly/Gly and 25 Gly/Trp) untreated hypertensive patients in basal conditions with the use of endogenous lithium concentration and uric acid. Fractional excretions of lithium and uric acid were significantly decreased in the Gly/Trp hypertensive patients compared with the Gly/Gly hypertensives. The contribution of alpha-adducin to fractional excretion of lithium was investigated by multiple regression analysis. Adducin genotype was significantly (R2=0.11, F=6.5; P<0.01) and directly related to fraction excretion of lithium; gender, age, urinary Na+, urinary uric acid, mean blood pressure, and plasma renin activity were not related. In conclusion, the adducin gene can be considered to be a 'renal hypertensive gene' that modulates the capacity of tubular epithelial cells to transport Na+ and hence contributes to the level of blood pressure.  (+info)

PST 2238: A new antihypertensive compound that modulates Na,K-ATPase in genetic hypertension. (2/1442)

A genetic alteration in the adducin genes is associated with hypertension and up-regulation of the expression of renal Na, K-ATPase in Milan-hypertensive (MHS) rats, in which increased ouabain-like factor (OLF) levels are also observed. PST 2238, a new antihypertensive compound that antagonizes the pressor effect of ouabain in vivo and normalizes ouabain-dependent up-regulation of the renal Na-K pump, was evaluated for its ability to lower blood pressure and regulate renal Na,K-ATPase activity in MHS genetic hypertension. In this study, we show that PST 2238, given orally at very low doses (1 and 10 microg/kg for 5-6 weeks), reduced the development of hypertension in MHS rats and normalized the increased renal Na,K-ATPase activity and mRNA levels, whereas it did not affect either blood pressure or Na,K-ATPase in Milan-normotensive (MNS) rats. In addition, a similar antihypertensive effect was observed in adult MHS rats after a short-term treatment. In cultured rat renal cells with increased Na-K pump activity at Vmax due to overexpression of the hypertensive variant of adducin, 5 days of incubation with PST 2238 (10(-10-)-10(-9) M) lowered the pump rate to the level of normal wild-type cells, which in turn were not affected by the drug. In conclusion, PST 2238 is a very potent compound that in MHS rats reduces blood pressure and normalizes Na-K pump alterations caused by a genetic alteration of the cytoskeletal adducin. Because adducin gene mutations have been associated with human essential hypertension, it is suggested that PST 2238 may display greater antihypertensive activity in those patients carrying such a genetic alteration.  (+info)

Transcriptional down-regulation of the rabbit pulmonary artery endothelin B receptor during phenotypic modulation. (3/1442)

1. We confirmed that endothelium-independent contraction of the rabbit pulmonary artery (RPA) is mediated through both an endothelin A (ET(A)R) and endothelin B (ET(B2)R) receptor. 2. The response of endothelium-denuded RPA rings to endothelin-1 (ET-1, pD2 = 7.84 +/- 0.03) was only partially inhibited by BQ123 (10 microM), an ET(A)R antagonist. 3. Pretreatment with 1 nM sarafotoxin S6c (S6c), an ET(B)R agonist, desensitized the ET(B2)R and significantly attenuated the response to ET-3 (pD2 = 7.40 +/- 0.02 before, <6.50 after S6c). 4. Pretreatment with S6c had little effect on the response to ET-1, but BQ123 (10 microM) caused a parallel shift to the right of the residual ETAR-mediated response to ET-1 (pD2 = 7.84 +/- 0.03 before S6c, 7.93 +/- 0.03 after S6c, 6.81 +/- 0.05 after BQ123). 5. Binding of radiolabelled ET-1 to early passage cultures of RPA vascular smooth muscle cells (VSMC) displayed two patterns of competitive displacement characteristic of the ET(A)R (BQ123 pIC50 = 8.73 +/- 0.05) or ET(B2)R (S6c pIC50 = 10.15). 6. Competitive displacement experiments using membranes from late passage VSMC confirmed only the presence of the ET(A)R (ET-1 pIC50 = 9.3, BQ123 pIC50 = 8.0, S6c pIC50 < 6.0). 7. The ET(A)R was functionally active and coupled to rises in intracellular calcium which exhibited prolonged homologous desensitization. 8. Using a reverse transcriptase polymerase chain reaction for the rabbit ET(B2)R, we demonstrated the absence of mRNA expression in phenotypically modified VSMC. 9. We conclude that the ET(B2)R expressed by VSMC which mediates contraction of RPA is rapidly down-regulated at the transcriptional level during phenotypic modulation in vitro.  (+info)

Interactions between neurogranin and calmodulin in vivo. (4/1442)

Neurogranin is a neural-specific, calmodulin (CaM)-binding protein that is phosphorylated by protein kinase C (PKC) within its IQ domain at serine 36. Since CaM binds to neurogranin through the IQ domain, PKC phosphorylation and CaM binding are mutually exclusive. Consequently, we hypothesize that neurogranin may function to concentrate CaM at specific sites in neurons and release free CaM in response to increased Ca2+ and PKC activation. However, it has not been established that neurogranin interacts with CaM in vivo. In this study, we examined this question using yeast two-hybrid methodology. We also searched for additional proteins that might interact with neurogranin by screening brain cDNA libraries. Our data illustrate that CaM binds to neurogranin in vivo and that CaM is the only neurogranin-interacting protein isolated from brain cDNA libraries. Single amino acid mutagenesis indicated that residues within the IQ domain are important for CaM binding to neurogranin in vivo. The Ile-33 --> Gln point mutant completely inhibited and Arg-38 --> Gln and Ser-36 --> Asp point mutants reduced neurogranin/CaM interactions. These data demonstrate that CaM is the major protein that interacts with neurogranin in vivo and support the hypothesis that phosphorylation of neurogranin at Ser-36 regulates its binding to CaM.  (+info)

Mechanical and chemical unfolding of a single protein: a comparison. (5/1442)

Is the mechanical unraveling of protein domains by atomic force microscopy (AFM) just a technological feat or a true measurement of their unfolding? By engineering a protein made of tandem repeats of identical Ig modules, we were able to get explicit AFM data on the unfolding rate of a single protein domain that can be accurately extrapolated to zero force. We compare this with chemical unfolding rates for untethered modules extrapolated to 0 M denaturant. The unfolding rates obtained by the two methods are the same. Furthermore, the transition state for unfolding appears at the same position on the folding pathway when assessed by either method. These results indicate that mechanical unfolding of a single protein by AFM does indeed reflect the same event that is observed in traditional unfolding experiments. The way is now open for the extensive use of AFM to measure folding reactions at the single-molecule level. Single-molecule AFM recordings have the added advantage that they define the reaction coordinate and expose rare unfolding events that cannot be observed in the absence of chemical denaturants.  (+info)

Identification of chURP, a nuclear calmodulin-binding protein related to hnRNP-U. (6/1442)

In a screen for myosin-like proteins in embryonic chicken brain, we have identified a novel nuclear protein structurally related to hnRNP-U (heterogeneous nuclear ribonuclear protein U). We have called this protein chURP, for chicken U-related protein. In this screen, chURP was immunoreactive with two myosin antibodies and, in common with the unconventional myosins, bound calmodulin in vitro in both the presence and absence of calcium ions. Determination of 757 amino acids of the chURP sequence revealed that it shares 41% amino acid identity with human and rat hnRNP-U, although chURP and hnRNP-U appear not to be orthologous proteins. ChURP is ubiquitously expressed in the nuclei of all chick tissues and, as one of a growing number of calmodulin-binding proteins to be identified in the nucleus, further highlights the potential of calmodulin as a regulator of nuclear metabolism.  (+info)

Identification of the calmodulin-binding domain of neuron-specific protein kinase C substrate protein CAP-22/NAP-22. Direct involvement of protein myristoylation in calmodulin-target protein interaction. (7/1442)

Various proteins in the signal transduction pathways as well as those of viral origin have been shown to be myristoylated. Although the modification is often essential for the proper functioning of the modified protein, the mechanism by which the modification exerts its effects is still largely unknown. Brain-specific protein kinase C substrate, CAP-23/NAP-22, which is involved in the synaptogenesis and neuronal plasticity, binds calmodulin, but the protein lacks any canonical calmodulin-binding domain. In the present report, we show that CAP-23/NAP-22 isolated from rat brain is myristoylated and that the modification is directly involved in its interaction with calmodulin. Myristoylated and non-myristoylated recombinant proteins were produced in Escherichia coli, and their calmodulin-binding properties were examined. Only the former bound to calmodulin. Synthetic peptides based on the N-terminal sequence showed similar binding properties to calmodulin, only when they were myristoylated. The calmodulin-binding site narrowed down to the myristoyl moiety together with a nine-amino acid N-terminal basic domain. Phosphorylation of a single serine residue in the N-terminal domain (Ser5) by protein kinase C abolished the binding. Furthermore, phosphorylation of CAP-23/NAP-22 by protein kinase C was also found myristoylation-dependent, suggesting the importance of myristoylation in protein-protein interactions.  (+info)

Phosphorylation of adducin by Rho-kinase plays a crucial role in cell motility. (8/1442)

Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and thereby promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes such as ruffling membranes. Rho-associated kinase (Rho- kinase), which is activated by the small guanosine triphosphatase Rho, phosphorylates alpha-adducin and thereby enhances the F-actin-binding activity of alpha-adducin in vitro. Here we identified the sites of phosphorylation of alpha-adducin by Rho-kinase as Thr445 and Thr480. We prepared antibody that specifically recognized alpha-adducin phosphorylated at Thr445, and found by use of this antibody that Rho-kinase phosphorylated alpha-adducin at Thr445 in COS7 cells in a Rho-dependent manner. Phosphorylated alpha-adducin accumulated in the membrane ruffling area of Madin-Darby canine kidney (MDCK) epithelial cells and the leading edge of scattering cells during the action of tetradecanoylphorbol-13-acetate (TPA) or hepatocyte growth factor (HGF). The microinjection of Botulinum C3 ADP-ribosyl-transferase, dominant negative Rho-kinase, or alpha-adducinT445A,T480A (substitution of Thr445 and Thr480 by Ala) inhibited the TPA-induced membrane ruffling in MDCK cells and wound-induced migration in NRK49F cells. alpha-AdducinT445D,T480D (substitution of Thr445 and Thr480 by Asp), but not alpha-adducinT445A,T480A, counteracted the inhibitory effect of the dominant negative Rho-kinase on the TPA-induced membrane ruffling in MDCK cells. Taken together, these results indicate that Rho-kinase phosphorylates alpha-adducin downstream of Rho in vivo, and that the phosphorylation of adducin by Rho-kinase plays a crucial role in the regulation of membrane ruffling and cell motility.  (+info)

*Calmodulin-binding proteins

... are, as their name implies, proteins which bind calmodulin. Examples include: Gap-43 protein ( ... Caldesmon Calmodulin-Binding Proteins at the US National Library of Medicine Medical Subject Headings (MeSH). ...

*Caldesmon

... is a protein that in humans is encoded by the CALD1 gene. Caldesmon is a calmodulin binding protein. Like calponin, ... This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and ... Sobue K, Muramoto Y, Fujita M, Kakiuchi S (Sep 1981). "Purification of a calmodulin-binding protein from chicken gizzard that ... The conserved domain of this protein possesses the binding activities to Ca++-calmodulin, actin, tropomyosin, myosin, and ...

*STRN

... nuclear autoantigen are members of a novel family of calmodulin-binding proteins that associate with protein phosphatase 2A". ... "Entrez Gene: STRN striatin, calmodulin binding protein". Goudreault M, D'Ambrosio LM, Kean MJ, Mullin MJ, Larsen BG, Sanchez A ... Gaillard S, Bartoli M, Castets F, Monneron A (Nov 2001). "Striatin, a calmodulin-dependent scaffolding protein, directly binds ... "A novel calmodulin-binding protein, belonging to the WD-repeat family, is localized in dendrites of a subset of CNS neurons". ...

*STRN3

... nuclear autoantigen are members of a novel family of calmodulin-binding proteins that associate with protein phosphatase 2A". J ... "Entrez Gene: STRN3 striatin, calmodulin binding protein 3". Goudreault M, D'Ambrosio LM, Kean MJ, Mullin MJ, Larsen BG, Sanchez ... and striatin are calmodulin-binding, WD repeat proteins principally expressed in the brain". J. Biol. Chem. 275 (26): 19970-7. ... "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3: 89. doi:10.1038/msb4100134 ...

*STRN4

"Entrez Gene: STRN4 striatin, calmodulin binding protein 4". Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom- ... Gaillard S, Bartoli M, Castets F, Monneron A (2001). "Striatin, a calmodulin-dependent scaffolding protein, directly binds ... and striatin are calmodulin-binding, WD repeat proteins principally expressed in the brain". J. Biol. Chem. 275 (26): 19970-7. ... "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/ ...

*Motor protein

Another motor protein essential for plant cell division is kinesin-like calmodulin-binding protein (KCBP), which is unique to ... Abdel-Gany I, Day IS, Simmons PK, Reddy AS (2005). "Origin and evolution of kinesin-like calmodulin-binding protein". Plant ... MBInfo - What are Motor Proteins? Ron Vale's seminar: "Cytoskeletal Motor Proteins" Biology of Motor Proteins Institute for ... Besides the motor proteins above, there are many more types of proteins capable of generating forces and torque in the cell. ...

*MATR3

Valencia CA, Ju W, Liu R (2007). "Matrin 3 is a Ca2+/calmodulin-binding protein cleaved by caspases". Biochem. Biophys. Res. ... 1998). "Mapping of human DNA-binding nuclear protein (NP220) to chromosome band 2p13.1-p13.2 and its relation to matrin 3". ... Matrin-3 is a protein that in humans is encoded by the MATR3 gene. The protein encoded by this gene is localized in the nuclear ... 2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. ...

*Plant calmodulin-binding domain

These domains are thought to constitute the calmodulin-binding domains of these proteins. Binding of the proteins to calmodulin ... the plant calmodulin-binding domain is a protein domain found repeated in a number of plant calmodulin-binding proteins. ... "Characterization of a pathogen-induced calmodulin-binding protein: mapping of four Ca2+-dependent calmodulin-binding domains". ... "Isolation and characterization of a novel calmodulin-binding protein from potato". J. Biol. Chem. 277 (6): 4206-14. doi:10.1074 ...

*NDUFAF4

Karp CM, Shukla MN, Buckley DJ, Buckley AR (2007). "HRPAP20: a novel calmodulin-binding protein that increases breast cancer ... NADH:ubiquinone oxidoreductase complex assembly factor 4 is a protein that in humans is encoded by the NDUFAF4 gene. NADH: ... interacting complex I assembly protein, cause fatal neonatal mitochondrial disease". Am. J. Hum. Genet. 84 (6): 718-27. doi: ...

*PCNT

Flory MR, Moser MJ, Monnat RJ, Davis TN (May 2000). "Identification of a human centrosomal calmodulin-binding protein that ... Flory MR, Moser MJ, Monnat RJ, Davis TN (May 2000). "Identification of a human centrosomal calmodulin-binding protein that ... PCNT has been shown to interact with: calmodulin, separase, CEP215, CHD3/4, protein kinase A, protein kinase C, DISC1, γ- ... This protein localizes to the centrosome and recruits proteins to the pericentriolar matrix (PCM) to ensure proper centrosome ...

*Transient receptor potential channel

Phillips AM, Bull A, Kelly LE (April 1992). "Identification of a Drosophila gene encoding a calmodulin-binding protein with ... Check date values in: ,access-date= (help) "TRIP Database". a manually curated database of protein-protein interactions for ... The identity of the mutated protein was unknown until it was cloned by Craig Montell, a post-doctoral researcher in Gerald ... TRPs have important interactions with other proteins and often form signaling complexes, the exact pathways of which are ...

*NPEPPS

Huber RJ, Catalano A, O'Day DH (January 2013). "Cyclin-dependent kinase 5 is a calmodulin-binding protein that associates with ... Glu 309 is one of the active site glutamates, and its mutation could convert the enzyme into an inactive binding protein. ... conversion of the enzyme into a catalytically inactive binding protein". Archives of Biochemistry and Biophysics. 413 (2): 236- ... The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkephalins in the brain, ...

*IQGAP1

This domain binds calmodulin, a protein known as a calcium sensor that can bind and regulate many target proteins. A GRD ( ... which mediates actin-binding and binds calponin. The WW, or poly-proline protein-protein domain, so named because of two ... Hart MJ, Callow MG, Souza B, Polakis P (Aug 1996). "IQGAP1, a calmodulin-binding protein with a rasGAP-related domain, is a ... Bashour AM, Fullerton AT, Hart MJ, Bloom GS (1997). "IQGAP1, a Rac- and Cdc42-binding protein, directly binds and cross-links ...

*RAC1

Hart MJ, Callow MG, Souza B, Polakis P (Jun 1996). "IQGAP1, a calmodulin-binding protein with a rasGAP-related domain, is a ... GTPase-activating-protein-related human protein IQGAP2 harbors a potential actin binding domain and interacts with calmodulin ... "Mapping the binding site for the GTP-binding protein Rac-1 on its inhibitor RhoGDI-1". Structure. 8 (1): 47-55. doi:10.1016/ ... "Interaction of Nck-associated protein 1 with activated GTP-binding protein Rac". The Biochemical Journal. 322 (3): 873-8. doi: ...

*Neurogranin

... is a calmodulin-binding protein expressed primarily in the brain, particularly in dendritic spines, and ... Neurogranin is the main postsynaptic protein regulating the availability of calmodulin, binding to it in the absence of calcium ... that corresponds to the protein kinase C phosphorylation site and the calmodulin-binding domain". J. Biol. Chem. 266 (229-237 ... Phosphorylation by protein kinase C lowers its binding ability. NRGN gene expression is controlled by thyroid hormones. Human ...

*Multiple-instance learning

Examples of where MIL is applied are: Molecule activity Predicting binding sites of Calmodulin binding proteins Predicting ... Multiple instance learning Multi-label classification Minhas, Fayyaz (2012). "Multiple instance learning of Calmodulin binding ... 2015) Predicting functional binding sites of MicroRNA targets Bandyopadhyay, Ghosh & et al. (2015) Medical image classification ... multiple instance learning for predicting specific functional binding sites in microRNA targets.", Sci. Rep., 5: 8004, doi: ...

*PPP2R2A

... nuclear autoantigen are members of a novel family of calmodulin-binding proteins that associate with protein phosphatase 2A". ... Ruediger R, Fields K, Walter G (Jan 1999). "Binding specificity of protein phosphatase 2A core enzyme for regulatory B subunits ... "Protein phosphatase 2A interacts with the 70-kDa S6 kinase and is activated by inhibition of FKBP12-rapamycinassociated protein ... "Direct activation of protein phosphatase-2A0 by HIV-1 encoded protein complex NCp7:vpr". FEBS Letters. 401 (2-3): 197-201. doi: ...

*PPAP2A

... nuclear autoantigen are members of a novel family of calmodulin-binding proteins that associate with protein phosphatase 2A". J ... 2003). "Protein phosphatase 2A (PP2A) regulates interleukin-4-mediated STAT6 signaling". J. Biol. Chem. 278 (5): 2787-2791. doi ... Pandey AV, Mellon SH, Miller WL (2003). "Protein phosphatase 2A and phosphoprotein SET regulate androgen production by P450c17 ... 1999). "Pharmacological characterization of protein phosphatase activities in preparations from failing human hearts". J. ...

*Index of biochemistry articles

... calcium-binding protein - Calmodulin - calmodulin-binding protein - Calvin cycle - CAM photosynthesis - CAM plants - cancer - ... protein - protein biosynthesis - Protein Data Bank - protein design - protein expression - protein folding - protein isoform - ... protein P16 - protein P34cdc2 - protein precursor - protein structure prediction - protein subunit - protein synthesis - ... ribosomal protein - ribosomal protein S6 kinase - ribosome - RNA - RNA virus - RNA-binding protein - RNA-directed DNA ...

*CAMK4

... protein kinase C, and calcium/calmodulin protein kinase; identification of flavin and calmodulin binding sites". J. Biol. Chem ... calmodulin-dependent protein kinase I by calmodulin and by Ca2+/calmodulin-dependent protein kinase kinase". J. Biol. Chem. 273 ... 1995). "Phosphorylation and activation of Ca2+-calmodulin-dependent protein kinase IV by Ca2+-calmodulin-dependent protein ... nuclear autoantigen are members of a novel family of calmodulin-binding proteins that associate with protein phosphatase 2A". J ...

*CDC42

Hart MJ, Callow MG, Souza B, Polakis P (June 1996). "IQGAP1, a calmodulin-binding protein with a rasGAP-related domain, is a ... GTPase-activating-protein-related human protein IQGAP2 harbors a potential actin binding domain and interacts with calmodulin ... "Analysis of RhoA-binding proteins reveals an interaction domain conserved in heterotrimeric G protein beta subunits and the ... "Cdc42-interacting protein 4 mediates binding of the Wiskott-Aldrich syndrome protein to microtubules". The Journal of ...

*Gap-43 protein

... protein F1 neuromodulin neural phosphoprotein B-50 axonal membrane protein GAP-43 calmodulin-binding protein P-57 nerve growth- ... it was shown to bind calmodulin avidly. GAP43, the consensus choice for its designation, is a nervous system-specific protein ... Gamby C, Waage MC, Allen RG, Baizer L (Oct 1996). "Analysis of the role of calmodulin binding and sequestration in neuromodulin ... Growth Associated Protein 43 also known as GAP43 is a protein that in humans is encoded by the GAP43 gene. GAP43 has been ...

*Thigmomorphogenesis

This is likely related to the calcium-binding protein calmodulin, suggesting Ca2+ involvement in mediating growth responses. ... Braam J, Davis RW (1990). "Rain-, wind-, and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis ... Erner, Y; Jaffe, MJ (1983). "Thigmomorphogenesis: Membrane lipid and protein changes in bean plants as affected by mechanical ...

*Calmodulin binding domain

In molecular biology, calmodulin binding domain (CaMBD) is a protein domain found in small-conductance calcium-activated ... They are heteromeric complexes that comprise pore-forming alpha-subunits and the Ca2+-binding protein calmodulin (CaM). CaM ... Channel opening is triggered when Ca2+ binds the EF hands in the N-lobe of CaM. The structure of this domain complexed with CaM ... This domain forms an elongated dimer with a CaM molecule bound at each end; each CaM wraps around three alpha-helices, two from ...

*SK channel

Calcium binds to the protein calmodulin (CaM). When bound to calcium, CaM binds to the CaM-binding domain on the intracellular ... When each of the four CaM-binding domain subunits is bound to calmodulin, the SK channel changes conformation. This transitions ... bound to the cytoplasmic C-terminus region of the peptide called the calmodulin binding domain (CaMBD). Additional association ... In addition, SK potassium channels are tightly associated with the protein calmodulin, which accounts for the calcium ...

*IQCB1

IQ calmodulin-binding motif-containing protein 1 is a protein that in humans is encoded by the IQCB1 gene. GRCh38: Ensembl ... and characterization of a p53 and DNA damage down-regulated gene PIQ that codes for a novel calmodulin-binding IQ motif protein ... a ciliary IQ domain protein, is mutated in Senior-Loken syndrome and interacts with RPGR and calmodulin". Nat Genet. 37 (3): ... 2002). "The gene mutated in juvenile nephronophthisis type 4 encodes a novel protein that interacts with nephrocystin". Nat. ...
Abstract. Caldesmon, an inhibitory actin binding protein, binds to actin and inhibits actin-myosin interactions, whereas caldesmon phosphorylation reverses the inhibitory effect of caldesmon on actin-myosin interactions, potentially leading to enhanced contraction. The goal of this study was to investigate the cellular signaling pathway responsible for caldesmon phosphorylation, which is involved in the regulation of the contraction induced by dexmedetomidine (DMT), an alpha-2 adrenoceptor agonist, in endothelium-denuded rat aortas. SP600125 (a c-Jun NH2-terminal kinase [JNK] inhibitor) dose-response curves were generated in aortas that were pre-contracted with DMT or phorbol 12,13-dibutyrate (PDBu), a protein kinase C (PKC) activator. Dose-response curves to the PKC inhibitor chelerythrine were generated in rat aortas pre-contracted with DMT. The effects of SP600125 and rauwolscine (an alpha-2 adrenoceptor inhibitor) on DMT-induced caldesmon phosphorylation in rat aortic vascular smooth muscle ...
TY - JOUR. T1 - Degradation of PEP-19, a calmodulin-binding protein, by calpain is implicated in neuronal cell death induced by intracellular Ca2+ overload. AU - Kanazawa, Y.. AU - Makino, M.. AU - Morishima, Y.. AU - Yamada, K.. AU - Nabeshima, T.. AU - Shirasaki, Y.. PY - 2008/6/23. Y1 - 2008/6/23. N2 - Excessive elevation of intracellular Ca2+ levels and, subsequently, hyperactivation of Ca2+/calmodulin-dependent processes might play an important role in the pathologic events following cerebral ischemia. PEP-19 is a neuronally expressed polypeptide that acts as an endogenous negative regulator of calmodulin by inhibiting the association of calmodulin with enzymes and other proteins. The aims of the present study were to investigate the effect of PEP-19 overexpression on cell death triggered by Ca2+ overload and how the polypeptide levels are affected by glutamate-induced excitotoxicity and cerebral ischemia. Expression of PEP-19 in HEK293T cells suppressed calmodulin-dependent signaling and ...
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The mammalian gene products, transient receptor potential (trp)1 to trp7, are related to the Drosophila TRP and TRP-like ion channels, and are candidate proteins underlying agonist-activated Ca2+-permeable ion channels. Recently, the TRP4 protein has been shown to be part of native store-operated Ca2+-permeable channels. These channels, most likely, are composed of other proteins in addition to TRP4. In the present paper we report the direct interaction of TRP4 and calmodulin (CaM) by: (1) retention of in vitro translated TRP4 and of TRP4 protein solubilized from bovine adrenal cortex by CaM-Sepharose in the presence of Ca2+, and (2) TRP4-glutathione S-transferase pull-down experiments. Two domains of TRP4, amino acid residues 688-759 and 786-848, were identified as being able to interact with CaM. The binding of CaM to both domains occurred only in the presence of Ca2+ concentrations above 10µM, with half maximal binding occurring at 16.6µM (domain 1) and 27.9µM Ca2+ (domain 2). Synthetic ...
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Erythrocyte membrane alterations mirror those of vascular smooth muscle and renal tubular cell membrane. The interaction between adducin and Na-K pump is the most likely biochemical mechanism responsible for the increased tubular Na reabsorption and hypertension in Milan hypertensive strain (MHS) rats. To substantiate this hypothesis in humans, we tested to see if α-adducin Gly460Trp genotype is associated with erythrocyte sodium transport rate in a new cohort of n268 never-treated North Sardinian primary hypertensives. Plasma renin activity and blood pressure response to hydrochlorothiazide were also measured to evaluate the relationship between sodium transport rate and two intermediate phenotypes with a higher degree of genetic complexity. Na-K pump, Na-K-Cl cotransport, and Li-Na countertransport at Vmax were faster (P,0.0001), whereas intracellular Na concentration was lower (P,0.0001) in patients carrying one or two 460Trp alleles. Such behavior was mirrored by opposite changes of ...
Sequence searching (5) revealed that 14 of the 39 calmodulin-binding proteins contain a motif whose consensus is (I/L)QXXK(K/X)GB, where X is any residue and B is a basic residue (Fig. 2B). A related sequence in myosins, IQXXXXKXXXR, has been shown previously to bind calmodulin (18). Thus, we demonstrate that the domain is found in many calmodulin-binding proteins. Presumably the other targets that lack this motif have other calmodulin-binding sequences (10).. In addition to the calmodulin-binding targets, we also identified one protein, Pyc1p, that bound Cy3-labeled streptavidin. Pyc1p encodes a pyruvate carboxylase 1 homolog that contains a highly conserved biotin attachment region (19). Thus, as predicted by its sequence, Pyc1p is biotinylated in vivo. With appropriate detection assays, we expect that proteome chips can identify many types of posttranslational modification of proteins.. To test whether proteome chips could be used to identify activities that might not be accessible by other ...
The effect of Ca2+ and calmodulin on phosphorylation of islet secretory granule proteins was studied. Secretory granules were incubated in a phosphorylation reaction mixture containing [32P]ATP and test reagents. The 32P-labeled proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the 32P content was visualized by autoradiography, and the relative intensities of specific bands were quantitated. When the reaction mixture contained EGTA and no added Ca2+, 32P was incorporated into two proteins with molecular weights of 45,000 and 13,000. When 10−4 M Ca2+ was added without EGTA, two additional proteins (58,000 and 48,000 Mr) were phosphorylated, and the 13,000-Mr protein was absent. The addition of 2.4 μM calmodulin markedly enhanced the phosphorylation of the 58,000- and 48,000-Mr proteins and resulted in the phosphorylation of a major protein whose molecular weight (64,000 Mr) is identical to that of one of the calmodulin binding proteins located on the granule ...
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008 ...
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dyes functional group. See the product information sheets for specific reactive dyes more information.. Coelenterazines and D-luciferin. Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also ...
We identified and analyzed 33 and 29 IQD1-like genes in Arabidopsis thaliana and Oryza sativa, respectively. The encoded IQD proteins contain a plant-specific domain of 67 conserved amino acid residues, referred to as the IQ67 domain, which is characterized by a unique and repetitive arrangement of three different calmodulin recruitment motifs, known as the IQ, 1-5-10, and 1-8-14 motifs. We demonstrated calmodulin binding for IQD20, the smallest IQD protein in Arabidopsis, which consists of a C-terminal IQ67 domain and a short N-terminal extension. A striking feature of IQD proteins is the high isoelectric point (~10.3) and frequency of serine residues (~11%). We compared the Arabidopsis and rice IQD gene families in terms of gene structure, chromosome location, predicted protein properties and motifs, phylogenetic relationships, and evolutionary history. The existence of an IQD-like gene in bryophytes suggests that IQD proteins are an ancient family of calmodulin-binding proteins and arose ...
We identified and analyzed 33 and 29 IQD1-like genes in Arabidopsis thaliana and Oryza sativa, respectively. The encoded IQD proteins contain a plant-specific domain of 67 conserved amino acid residues, referred to as the IQ67 domain, which is characterized by a unique and repetitive arrangement of three different calmodulin recruitment motifs, known as the IQ, 1-5-10, and 1-8-14 motifs. We demonstrated calmodulin binding for IQD20, the smallest IQD protein in Arabidopsis, which consists of a C-terminal IQ67 domain and a short N-terminal extension. A striking feature of IQD proteins is the high isoelectric point (~10.3) and frequency of serine residues (~11%). We compared the Arabidopsis and rice IQD gene families in terms of gene structure, chromosome location, predicted protein properties and motifs, phylogenetic relationships, and evolutionary history. The existence of an IQD-like gene in bryophytes suggests that IQD proteins are an ancient family of calmodulin-binding proteins and arose ...
adducin: membrane-skeleton associated calmodulin-binding protein of erythrocytes; major substrate for Ca+- & phospholipid-dependent protein kinase C; alpha-beta heterodimer with subunits of MW 103kDa (alpha) & 97kDa (beta); human erythrocyte GenBank X58199; alternatively spliced human transcript GenBank U43959
The A and C subunits of PP2A were previously shown to bind to striatin family complexes but the region of striatin to which the A/C heterodimer bound was unknown [8]. Our current data show that the coiled-coil domain of striatin mediates the formation of this PP2A heterotrimer (Figure 10). Although we cannot rule out a direct contribution of the caveolin-binding motif, the fact that double or triple point mutations in the middle of the coiled-coil/oligomerization domain of striatin almost completely disrupt PP2A association suggests that the primary determinants for PP2A binding are near the middle of the coiled-coil domain (residues 84-105).. Our data also suggest that PP2A association with striatin is dependent on oligomerization of striatin complexes. Of the charged coiled-coil residues that were mutated to disrupt PP2A binding to striatin, only arginine 88 is predicted to be at the dimerization interface when either NCOILS [33] or Paircoil2 [34] prediction programs are used, while the other ...
ConclusionThis study suggested that the C allele of XRCC1 had an 18% significantly decreased risk of TC in Chinese, and there were no significant associations among Caucasians under all genetic models....
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p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Caldesmon interaction with smooth muscle myosin and its ability to cross-link actin filaments to myosin were investigated by the use of several bacterially expressed myosin-binding fragments of caldesmon. We have confirmed the presence of two functionally different myosin-binding sites located in domains 1 and 3/4a of caldesmon. The binding of the C-terminal site is highly sensitive to ionic strength and hardly participates in acto-myosin cross-linking, while the N-terminal binding site is relatively independent of ionic strength and apparently contains two separate myosin contact regions within residues 1-28 and 29-128 of chicken gizzard caldesmon. Both these N-terminal sub-sites are involved in the interaction with myosin and are predominantly responsible for the caldesmon-mediated high-affinity cross-linking of actin and myosin filaments, without affecting the affinity of direct acto-myosin interaction. Binding of caldesmon and its fragments to myosin or rod filaments revealed affinity in the ...
BACKGROUND: Activation and consequent phenotypic modulation of mesangial cells is considered to play a crucial role in the process of glomerular disease progression. Caldesmon, a calmodulin and actin-binding protein, is a molecular marker of the phenotypic change in smooth-muscle cells. SUBJECTS AND METHODS: We studied whether the expression of caldesmon in mesangial cells was enhanced in the process of IgA nephropathy and whether it would be a marker of mesangial activation indicating prognostic significance in specific disease states. We performed immunohistochemical staining with anticaldesmon and alpha-smooth-muscle actin (alpha-SMA) antibodies in 32 biopsy specimens from IgA nephropathy patients and analysed them quantitatively with a computer-aided manipulator. RESULTS: The glomerular expression of caldesmon was enhanced in IgA nephropathy patients. We compared caldesmon expression with composite histological scores (cell score and matrix score), clinical parameters and expressions of ...
The calcium-signaling network is an important transducer of internal and external stimuli in plants. These signals are transduced by a divers set of calcium-sensors such as calmodulin and calmodulin like proteins. In this work, AFG1L2 (AFG1 like protein 2) was characterized as a calmodulin binding protein that belongs to the family of AAA+ proteins (ATPases associated with various cellular activities). Using GFP fusion constructs it was possible to determine the in vivo localization of AFG1L2 in mitochondria and also to confirm the dual localization of a previously described homologe, AFG1L1, to mitochondria and chloroplasts. The interaction between AFG1L2 and calmodulin is calcium dependent and the calmodulin binding site of the AFG1L2 protein is in the AAA domain at a site homologous to the AFG1L1 protein, in very close proximity to the Walker A Motif, which is essential for ATP hydrolysis. It could be shown that ATP and ADP intensify the interaction between AFG1L2 and calmodulin. AAA-proteins ...
Transcription factor regulating the cell cycle specific transcription of a spindle pole body (SPB) calmodulin binding protein SPC110. Required for full induction of SPC110 transcription in late G1. Binds to DNA consensus sequence 5-[AT]AA[TC]AAACAA[AT]-3. Dosage dependent suppressor of calmodulin mutants which have specific defects in SPB assembly.
The binding of chicken gizzard caldesmon to actin was studied both in the presence and the absence of caltropin using Airfuge centrifugation experiments, disulfide cross-linking studies, and the fluorescent probe acrylodan (6-acryloyl-2-(dimethylamino)naphthalene). In co-sedimentation studies most of the caldesmon pelleted along with actin. However, when caldesmon in the presence of caltropin was mixed with actin, caldesmon did not pellet along with actin following high speed centrifugation, suggesting that caltropin has significantly weakened its binding to actin. The caltropin effect was noticed even when tropomyosin was included in the reaction mixture. Acrylodan-labeled caldesmon, when excited at 375 nm, had an emission maximum at 515 +/- 2 nm. The addition of actin produced a nearly 70% increase in fluorescent intensity, accompanied by a blue shift in the emission maximum (i.e. lambda em (max) = 505 +/- 2 nm), suggesting that the probe now occupies a more nonpolar environment. Titration of labeled
Alpha-adducin is a protein that in humans is encoded by the ADD1 gene. Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. Polymorphism in ADD1 is associated with hypertension. GRCh38: Ensembl release 89: ENSG00000087274 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000029106 - Ensembl, May 2017 "Human PubMed ...
Adducin is a protein recently purified from erythrocytes and brain that has properties in in vitro assays suggesting a role in assembly of a spectrin-actin lattice. This report describes the localization of adducin to plasma membranes of a variety of tissues and the discovery that adducin is concentrated at sites of cell-cell contact in the epithelial tissues where it is expressed. Adducin in tissues and cultured cells always was observed in association with spectrin and actin, although spectrin and actin were evident in the absence of adducin. In sections of intestinal epithelial cells spectrin was present on all plasma membrane surfaces while adducin was restricted to the lateral cell borders. Adducin also was not detected in association with actin stress fibers in cultured cells. The presence of adducin at cell-cell contact sites of cultured epithelial cells requires extracellular Ca++ and occurs within 15 min of addition of 0.3 mM Ca++. Redistribution of adducin after addition of ...
The key finding of our study was that in a randomly recruited sample of middle-aged and older white subjects, ACE DD homozygosity predicted total and cardiovascular mortality and the incidence of cardiovascular events in carriers of the mutated ADD1 but not in subjects carrying the wild-type ADD1. These findings translate previously observed11-15 associations of cardiovascular risk factors with these 2 polymorphisms into hard cardiovascular outcomes. Furthermore, in ex vivo studies, human fibroblasts showed higher membrane-bound ACE activity in the presence of the ADD1 Trp allele. We corroborated the latter observation in cell membranes of HEK cells transfected with the human mutated and wild-type ADD1. Based on this replication, we believe that our ex vivo finding might be extrapolated to human kidney cells.. In line with expert opinion,24 we opted for the candidate gene approach, which relies on solid knowledge of pathophysiologic pathways and accounts for the context dependency of genetic ...
目的:探讨浅表性平滑肌肉瘤(superficial leiomyosarcoma,SLMS)的临床病理特点、诊断、鉴别诊断及治疗和预后。方法:回顾性分析13例SLMS的临床病理特征,并复习相关文献讨论。结果:男7例,女6例,平均年龄59岁。肿瘤位于四肢7例,躯干3例,头面部2例,阴囊1例,其中位于真皮的9例,皮下4例。肿瘤直径1~8(平均4.3)cm。局部复发5例,无远处转移病例。镜下:肿瘤呈结节状或弥漫状生长,根据肿瘤细胞不同分化分为三型:中分化(最多见,9例)、高分化和低分化型(较少,各2例)。免疫组织化学:肿瘤细胞desmin,MSA,a-SMA和h-caldesmon阳性。结论:SLMS多发生于中老年人,预后较好,易发生局部复发,极少发生远处转移。治疗采用局部扩大切除。
Ca2+-calmodulin binding to caldesmon and the caldesmon-actin-tropomyosin complex. Its role in Ca2+regulation of the activity of synthetic smooth-muscle thin filaments Academic Article ...
Related Articles The predictive performance of short-linear motif features in the prediction of calmodulin-binding proteins. BMC Bioinformatics. 2018 Nov 20;19(Suppl 14):410 Authors: Li Y, Maleki ...
Heterogeneity of SMC is a well-known phenomenon. The interpretation, however, of the morphologic phenotype as well as the functional implications of the expression of differentiation markers is subject to much debate. The data are derived from in vivo or in vitro studies. The material used varies from adult to neonatal to fetal vessels and cells from different animal species. The origin of the cultured cells can be from intima, inner or outer media, or the adventitia in both normal and diseased vessels.. The data from in vitro studies are largely reflected in in vivo studies. Proceeding from data in early fetal development, it has been shown that the SMC start to express SM α-actin17 followed by 1E12,20 an actinin marker, and smoothelin.21 Relative late differentiation markers are the already mentioned markers, such as SM22, calponin, h-caldesmon,7 and SM myosin.8 These are upregulated until the expression level of the mature vessel wall is reached.9 11 During this developmental period, there ...
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Calcineurin B兔多克隆抗体(ab94535)可与人样本反应并经WB实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Plasmid Actin-53b-ires-EGFP from Dr. Jerry Crabtrees lab contains the insert BAF53b and is published in Neuron. 2007 Oct 4. 56(1):94-108. This plasmid is available through Addgene.
What function might a flagellar membrane-associated CrKCBP have? One function might include a role in intraflagellar transport (IFT). IFT involves a plus-end-directed heterotrimeric kinesin as well as a minus-end-directed cytoplasmic dynein and is required for the assembly, disassembly and structural maintenance of cilia and flagella (for a review, see Cole, 2003). Presumably, a minus-end-directed kinesin (CrKCBP) and cytoplasmic dynein would have redundant functions in IFT. However, it is possible that two minus-end-directed motors are required during rapid flagellar resorption. In Chlamydomonas, flagellar resorption can be induced by removal of Ca2+ from the medium and is reversed by re-addition of Ca2+ to the medium (Lefebvre et al., 1978). If CrKCBP is negatively regulated by Ca2+-calmodulin in the same way as AtKCBP (see Song et al., 1997), removal of Ca2+ from the medium would activate KCBP, consistent with a role for CrKCBP during flagellar resorption.. Several key observations provide ...
Sigma-Aldrich offers abstracts and full-text articles by [Jampani Nageswara Rao, Roland Rivera-Santiago, Xiaochuan Edward Li, William Lehman, Roberto Dominguez].
connectin: cell surface protein which binds both laminin & actin; binds thick filaments to Z lines in frog skeletal muscle; see also record for mini-titin which is a similar protein from invertebrates; titin has a protein kinase domain and a calmodulin-binding site near the C-terminus
A membrane skeleton protein (heterodimer) that interacts with a junctional complex that links spectrin assemblies. The complex in the red blood cell consists of tropomyosin and actin with band 4.1 (see band). Adducin probably binds to band 4.1.. [...] ...
Complete information for CALD1 gene (Protein Coding), Caldesmon 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Calcineurin A; One Isoform (the Other Is Cmp2p) Of The Catalytic Subunit Of Calcineurin, A Ca++/calmodulin-regulated Protein Phosphatase Which Regulates Crz1p (a Stress-response Transcription Factor), The Other Calcineurin Subunit Is CNB1; Regulates The Function Of Aly1p Alpha-arrestin; CNA1 Has A Paralog, CMP2, That Arose From The Whole Genome Duplication
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Calponin兔多克隆抗体(ab111183)可与人样本反应并经WB, IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Microtubules (MTs) and actin filaments (F-actin) function cooperatively to regulate plant cell morphogenesis. However, the mechanisms underlying the crosstalk between these two cytoskeletal systems, particularly in cell shape control, remain largely unknown. Here, we show that introduction of the MyTH4-FERM tandem into KCBP (Kinesin-like Calmodulin-Binding Protein) during evolution conferred novel functions. The MyTH4 domain and the FERM domain in the N-terminal tail of KCBP physically bind to MTs and F-actin, respectively. During trichome morphogenesis, KCBP distributes in a specific cortical gradient, and concentrates at the branching sites and the apexes of elongating branches, which lack MTs but have cortical F-actin. Further live-cell imaging and genetic analyses revealed that KCBP acts as a hub integrating MTs and actin filaments to assemble the required cytoskeletal configuration for the unique, polarized diffuse growth pattern during trichome cell morphogenesis. Our findings provide ...
The calponin family of F-actin-, tropomyosin- and calmodulin-binding proteins currently comprises three genetic variants. Their functional roles implicated from in vitro studies include the regulation of actomyosin interactions in smooth muscle cells (h1 calponin), cytoskeletal organisation in non-muscle cells (h2 calponin) and the control of neurite outgrowth (acidic calponin). We have now investigated the effects of calponin (CaP) isoforms and their C-terminal deletion mutants on the actin cytoskeleton by time lapse video microscopy of GFP fusion proteins in living smooth muscle cells and fibroblasts. It is shown that h1 CaP associates with the actin stress fibers in the more central part of the cell, whereas h2 CaP localizes to the ends of stress fibres and in the motile lamellipodial protrusions of spreading cells. Cells expressing h2 CaP spread more efficiently than those expressing h1 CaP and expression of GFP h1 CaP resulted in reduced cell motility in wound healing experiments. Notably, ...
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Adducin is a membrane-skeletal protein which is a candidate to promote assembly of a spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. The complete sequence of both subunits of human adducin, alpha (737 amino acids), and beta (726 amino acids) has been deduced by analysis of the cDNAs. The two subunits have strikingly conserved amino acid sequences with 49% identity and 66% similarity, suggesting evolution by gene duplication. Each adducin subunit has three distinct domains: a 39-kD NH2-terminal globular protease-resistant domain, connected by a 9-kD domain to a 33-kD COOH-terminal protease-sensitive tail comprised almost entirely of hydrophilic amino acids. The tail is responsible for the high frictional ratio of adducin noted previously, and was visualized by EM. The head domains of both adducin subunits exhibit a limited sequence similarity with the NH2-terminal actin-binding motif present in members of the spectrin superfamily and actin gelation ...
Calcineurin (CN), a calcium- and calmodulin-dependent protein phosphatase, plays a significant role in the central nervous system. Previously, we reported that forebrain-specific CN knockout mice (CN mutant mice) have impaired working memory. To further analyze the behavioral effects of CN deficiency, we subjected CN mutant mice to a comprehensive behavioral test battery. Mutant mice showed increased locomotor activity, decreased social interaction, and impairments in prepulse inhibition and latent inhibition. In addition, CN mutant mice displayed an increased response to the locomotor stimulating effects of MK-801. Collectively, the abnormalities of CN mutant mice are strikingly similar to those described for schizophrenia. We propose that alterations affecting CN signaling could comprise a contributing factor in schizophrenia pathogenesis.. ...
This report describes Ca2+-dependent binding of 125I-labeled calmodulin (125I-CaM) to erythrocyte membranes and identification of two new CaM-binding proteins. Erythrocyte CaM labeled with 125I-Bolton Hunter reagent fully ...
Two neuron-specific proteins, neurogranin (Ng), and neuromodulin (GAP43), were studied. Our first aim was to know the potentiality of various chemicals for their induction of Ng mRNA expression; our second one was to know the intracellular localization of GAP43 and its co-localization with calmodulin (CaM). Our results indicated that although some of the NO donors could enhance Ng promoter activity, they could not induce Ng mRNA expression in HEK293 and PC12 cells. Moreover, our findings revealed that Ng mRNA could be induced only by NGF and only in PC12 cells. For GAP43b s intracellular localization and co-localization with CaM, our results revealed DsRed-GAP43 and its mutants (S41D, S41G) were expressed mainly in the region near to cell membrane while EGFP-GAP43 and its mutants (S41D, S41G) were mostly distributed in cytoplasm. PMA treatment did not affect the localization of GAP43. For the co-transfection experiments, a particular region of co-localization of GAP43 and CaM was found to be ...
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NRGN - NRGN (untagged)-Human neurogranin (protein kinase C substrate, RC3) (NRGN), transcript variant 1 available for purchase from OriGene - Your Gene Company.
View mouse Camta1 Chr4:151059525-151861876 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
EWSR1 - EWSR1 (Myc-DDK-tagged)-Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 1 available for purchase from OriGene - Your Gene Company.
Lapin Calcineurin B Polyclonal anticorps pTyr106 pour IHC (p), WB. Commandez anti-Calcineurin B anticorp. | Numéro de produit ABIN1712443
1. Huang KP, Huang FL, Jager T, Li J, Reymann KG, Balschun D. Neurogranin/RC3 enhances long-term potentiation and learning by promoting calcium-mediated signaling. J Neurosci. 2004;24:10660-10669 2. Baudier J, Deloulme JC, Van Dorsselaer A, Black D, Matthes HW. Purification and characterization of a brain-specific protein kinase C substrate, neurogranin (p17). Identification of a consensus amino acid sequence between neurogranin and neuromodulin (GAP43) that corresponds to the protein kinase C phosphorylation site and the calmodulin-binding domain. J Biol Chem. 1991;266:229-237 3. Sheu FS, Mahoney CW, Seki K, Huang KP. Nitric oxide modification of rat brain neurogranin affects its phosphorylation by protein kinase C and affinity for calmodulin. J Biol Chem. 1996;271:22407-22413 4. Cohen RW, Margulies JE, Coulter PM 2nd, Watson JB. Functional consequences of expression of the neuron-specific, protein kinase C substrate RC3 (neurogranin) in Xenopus oocytes. Brain Res. 1993;627:147-152 5. Yang HM, ...
TY - JOUR. T1 - Fluorescence analysis of calmodulin mutants containing tryptophan. T2 - Conformational changes induced by calmodulin-binding peptides from myosin light chain kinase and protein kinase II. AU - Prendergast, Franklyn G.. PY - 1991. Y1 - 1991. N2 - Peptide-induced conformational changes in five isofunctional mutants of calmodulin (CaM), each bearing a single tryptophan residue either at the seventh position of each of the four calcium-binding loops (i.e., amino acids 26, 62, 99, and 135) or in the central helix (amino acid 81) were studied by using fluorescence spectroscopy. The peptides RS20F and RS20CK correspond to CaM-binding amino acid sequence segments of either nonmuscle myosin light chain kinase (nmMLCK) or calmodulin-dependent protein kinase II (CaMPK-II), respectively. Both steady-state and time-resolved fluorescence data were collected from the various peptide-CaM complexes. Steady-state fluorescence intensity measurements indicated that, in the presence of an excess of ...
Results The combination of ER+/PR+/CD10+/GEM−/h-caldesmon−/transgelin− can predict ESS versus ULMS with AUC predictive value of 0.872 (95% CI 0.784 to 0.961, p,0.0001). The combination of ER+/PR+/CD10+/h-caldesmon−/transgelin− can predict low grade (LG) ESS from LG ULMS with AUC predictive value of 0.914 (95% CI 0.832 to 0.995, p,0.0001). Finally, ULMS and ESS, including the LGs, were more likely to be stathmin1+ than LM.. ...
The PMC1 gene in Saccharomyces cerevisiae encodes a vacuolar Ca2+ ATPase required for growth in high-Ca2+ conditions. Previous work showed that Ca2+ tolerance can be restored to pmc1 mutants by inactivation of calcineurin, a Ca2+/calmodulin-dependent protein phosphatase sensitive to the immunosuppressive drug FK506. We now report that calcineurin decreases Ca2+ tolerance of pmc1 mutants by inhibiting the function of VCX1, which encodes a vacuolar H+/Ca2+ exchanger related to vertebrate Na+/Ca2+ exchangers. The contribution of VCX1 in Ca2+ tolerance is low in strains with a functional calcineurin and is high in strains which lack calcineurin activity. In contrast, the contribution of PMC1 to Ca2+ tolerance is augmented by calcineurin activation. Consistent with these positive and negative roles of calcineurin, expression of a vcx1::lacZ reporter was slightly diminished and a pmc1::lacZ reporter was induced up to 500-fold by processes dependent on calcineurin, calmodulin, and Ca2+. It is likely ...
To capture the Myo1p-interacting proteins for in vitro validation of the physical interactions identified by iMYTH, and for their subsequent identification by coimmunoprecipitation (coIP), we employed a modified pull-down approach (Babu et al. 2009) using a TAP epitope fused to the Myo1p C-terminus (Myo1-TAP). The Myo1-TAP bait strain was transformed with a full-length prey protein in expression vector BG1805, a URA3 multicopy 2 μ plasmid containing a GAL1 promoter (Gelperin et al. 2005), using the standard lithium acetate procedure (Gietz and Woods 2006). The Myo1 protein was captured using the calmodulin-binding peptide in the TAP epitope. Briefly, 40 ml of culture was grown for 2 d in YPD at 30° at 200 rpm. Subsequently, the cells were centrifuged at 3000 rpm for 3 min, and washed twice with 20 ml IPLB buffer (20 mM Hepes KOH, pH 7.4, 150 mM KOAc, 2 mM Mg(Ac)2, 2 mM CaCl2, and 10% glycerol). For plasmid expression, cells were resuspended in YP-GAL, and incubated for 2-4 hr. The yeast cells ...
N-methyl-D-aspartate (NMDA) receptors are calcium-permeable ion channels assembled from four subunits that each have a common membrane topology. The intracellular carboxyl terminal domain (CTD) of each subunit varies in length, is least conserved between subunits, and binds multiple intracellular proteins. We defined a region of interest in the GluN2A CTD, downstream of well-characterized membraneproximal motifs, that shares only 29% sequence similarity with the equivalent region of GluN2B. GluN2A (amino acids 875-1029) was fused to GST and used as a bait to identify proteins from mouse brain with the potential to bind GluN2A as a function of calcium. Using mass spectrometry we identified calmodulin as a calcium-dependent GluN2A binding partner. Equilibrium fluorescence spectroscopy experiments indicate that Ca²⁺/calmodulin binds GluN2A with high affinity (5.2 ± 2.4 nM) in vitro. Direct interaction of Ca²⁺/calmodulin with GluN2A was not affected by disruption of classic sequence motifs ...
I need an online or downloadable hydropathy plot programme. But I need something special! I want a programme that takes into account the presence or absence of non-covalent modifications, especially phosphorylation sites and calmodulin binding sites. Any suggestions? If so, please email me at: o.s.depeyer at reading.ac.uk ...
We investigated the participation of telomerase and the factors responsible for its activation or suppression in the fibrotic process found in pulmonary specimens obtained by surgical lung biopsy from patients with IPF. Specifically, we investigated telomerase and ET-1 expression in alveolar epithelium, endothelium, and myofibroblast cells present in normal lung parenchyma, minimal (fibroblast foci) fibrosis and vascular areas of UIP to verify the impact of these markers on the remodeling/fibrosis process. In the present study, telomerase and ET-1 were significantly increased in normal lung parenchyma and vascular areas compared to fibroblast foci areas of UIP. Unexpectedly, myofibroblast α-SMA+ expression was more prominent than smooth muscle caldesmon+ cells in vessels. Interestingly, we found a positive association between telomerase and ET-1 in UIP vascular areas, whereas a negative association was found between telomerase and ET-1 in normal lung parenchyma. A positive association was found ...
The vacuolar calmodulin (CaM)-stimulated Ca2+-ATPase, BCA1p, in cauliflower (Brassica oleracea) has an extended N terminus, which was suggested to contain a CaM-binding domain (S. Malmstrom, P. Askerlund, M.G. Palmgren [1997] FEBS Lett 400: 324328). The goal of the present study was to determine the role of the N terminus in regulating BCA1p. Western analysis using three different antisera showed that the N terminus of BCA1p is cleaved off by trypsin and that the N terminus contains the CaM-binding domain. Furthermore, the expressed N terminus binds CaM in a Ca2+dependent manner. A synthetic peptide corresponding to the CaM-binding domain of BCA1p (Ala-19 to Leu-43) strongly inhibited ATP-dependent Ca2+ pumping by BCA1p in cauliflower low-density membranes, indicating that the CaM-binding region of BCA1p also has an autoinhibitory function. The expressed N terminus of BCA1p and a synthetic peptide (Ala-19 to Met-39) were good substrates for phosphorylation by protein kinase C. Sequencing of the ...
Knocking down the striatin gene in mice and polymorphic variants of the striatin gene in humans is associated with SS of BP. In the mice, SS is not related to an alteration in aldosterone production but may be related to alteration in its metabolism and effectiveness. MR expression in the kidney is increased as are the levels of expression of 2 downstream targets of MR activation, SGK1 and especially ENaC. Importantly, in both the kidney and the heart, a signature for a defect in aldosterones rapid, nongenomic pathway-decrease phosphorylation of Akt-is present. From this and a previous study, we have documented that dietary sodium restriction (that increases aldosterone) and direct aldosterone administration increases MR expression. Thus, on a liberal salt diet, one would expect a decrease in the expression of MR, SGK1, and ENaC. This did not occur in Strn+/− mice, suggesting that striatin plays a role, as yet undefined, in modulating the normal effect of salt intake on this cascade. The net ...
Study to Evaluate the Value of the Follow-up of CALCIneurin Activity to MOdulate Calcineurin Inhibitors-induced Immunosuppression in Lung Transplantation
Now lets look back at the exciting findings of Pinkstaff et al. 2001. They found IRES-dependent translation associated with the mRNAs of Arc, CaMKII, neurogranin, MAP2 and dendrin. Did they run these experiments well enough for Kozaks standards? First, they used the pRF construct. We know that baseline firefly luciferase expression in this dicistronic system is variable. Unfortunately, we cant tell how variable it is in these experiments because all results are expressed as a ratio of Renilla to firefly levels normalized to the negative control baseline vector. Kozak defines as weak any increase of less than 5-fold compared to the control vector. CaMKII and dendrin never cross this threshold, so it would be crucial to make sure these arent varying at the level explained by baseline fluctuations. The initial RNA analyses for neurogranin turned up two small RNAs, indicating that a splice site had been inserted. After removing this issue, neurogranin still had some of the highest IRES ...
The goal of Dr. Picciottos research team is to understand the role of single molecules in complex behaviors related to addiction, depression, feeding and learning. She and her colleagues use molecular genetic and pharmacological approaches to link the biochemical, cellular, and anatomical levels of investigation to behavior. Of primary interest is the role of acetylcholine and nicotinic acetylcholine receptors in brain function and development.. Dr. Picciottos laboratory also studies signaling molecules downstream of nicotinic receptors, such as calcineurin, CaM kinases and adducins, which may mediate long-term changes in behavior downstream of these receptors.. Specialized Terms: Neuroscience; Molecular basis of behavior; Intracellular signaling; Mouse genetic ...
4J9Z: Unstructured to structured transition of an intrinsically disordered protein peptide in coupling Ca2+-sensing and SK channel activation.
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Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects ...
There are 13 calories in 9 grams of Chicken Gizzard (Simmered, Cooked). Get full nutrition facts and other common serving sizes of Chicken Gizzard (Simmered, Cooked) including 1 oz and 100 g.
June 1, 2009 , Article Chi Nail Bar, located right off the well-known shopping mecca that is Rodeo Drive, opened this February. I think we can all admit that its not exactly the ideal time to open up a new business, what with the economic crisis and all. But that didnt stop Ton, a second generation nail tech, from opening up her newest salon. In the business for 10 years, Ton owned several other salons before coming across the space in chic Beverly Hills.. Keywords: gel overlays nails-only salons On The Road salon decor/design Vietnamese salon industry ...
Max has been hankering for gizzards for sometime but I havent had much luck finding them until a couple of weeks ago. Gizzards are tough muscle meat
This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction ...
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Looking for online definition of CaM-kinase II alpha chain in the Medical Dictionary? CaM-kinase II alpha chain explanation free. What is CaM-kinase II alpha chain? Meaning of CaM-kinase II alpha chain medical term. What does CaM-kinase II alpha chain mean?
Calcineurin (CaN) is a calcium and calmodulin dependent serine/threonine protein phosphatase (also known as protein phosphatase 3, and calcium-dependent serine-threonine phosphatase). It activates the T cells of the immune system and can be blocked by drugs. Calcineurin activates nuclear factor of activated T cell, cytoplasmic (NFATc), a transcription factor, by dephosphorylating it. The activated NFATc is then translocated into the nucleus, where it upregulates the expression of interleukin 2 (IL-2), which, in turn, stimulates the growth and differentiation of T cell response. Calcineurin is the target of a class of drugs called calcineurin inhibitors, which includes cyclosporin, voclosporin, pimecrolimus and tacrolimus. Calcineurin is a heterodimer of a 61-kD calmodulin-binding catalytic subunit, calcineurin A and a 19-kD Ca2+-binding regulatory subunit, calcineurin B. There are three isozymes of the catalytic subunit, each encoded by a separate gene (PPP3CA, PPP3CB, and PPP3CC) and two ...
STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo[4] "Regulated mesoderm migration is necessary for the proper morphogenesis and organ formation during embryonic development. Cell migration and its dependence on the cytoskeleton and signaling machines have been studied extensively in cultured cells; in contrast, remarkably little is known about the mechanisms that regulate mesoderm cell migration in vivo. Here, we report the identification and characterization of a mouse mutation in striatin-interacting protein 1 (Strip1) that disrupts migration of the mesoderm after the gastrulation epithelial-to-mesenchymal transition (EMT). STRIP1 is a core component of the biochemically defined mammalian striatin-interacting phosphatases and kinase (STRIPAK) complexes that appear to act through regulation of protein phosphatase 2A (PP2A), but their functions in mammals in vivo have not been examined. Strip1-null mutants arrest development at ...
STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo[4] "Regulated mesoderm migration is necessary for the proper morphogenesis and organ formation during embryonic development. Cell migration and its dependence on the cytoskeleton and signaling machines have been studied extensively in cultured cells; in contrast, remarkably little is known about the mechanisms that regulate mesoderm cell migration in vivo. Here, we report the identification and characterization of a mouse mutation in striatin-interacting protein 1 (Strip1) that disrupts migration of the mesoderm after the gastrulation epithelial-to-mesenchymal transition (EMT). STRIP1 is a core component of the biochemically defined mammalian striatin-interacting phosphatases and kinase (STRIPAK) complexes that appear to act through regulation of protein phosphatase 2A (PP2A), but their functions in mammals in vivo have not been examined. Strip1-null mutants arrest development at ...
Kaupp, U.B., Niidome, T., Tanabe, T., Terada, S., Bonigk, W., Stuhmer, W., Cook, N.J., Kangawa, K., Matsuo, H., Hirose, T. 1989 Primary structure and functional expression from complementary DNA of the rod photoreceptor cyclic GMP-gated channel. Nature, 342, 762 766 ...
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I spend most of my time fishing for channels with chicken livers. I recently noticed that chicken gizzards were cheaper and am wondering if anyone has...
Sofrit, or chicken gizzards & hearts in hot sauce recipe. A popular dish in RI. Dip chunks of Italian bread in sofrit and savor tender bites of chicken.
Spicy Chicken Gizzards is a fantastic dish for a Friday evening over a couple of beers ;). Lets call it an apt starter with drinks.
Rabbit recombinant monoclonal Caldesmon antibody [EP789Y] validated for WB, IP, IHC, Flow Cyt, ICC/IF and tested in Human. Referenced in 2 publications.
(1994) Means. FEBS Letters. Calcium and its ubiquitous intracellular receptor calmodulin are required for cell proliferation. Studies in a variety of model systems are beginning to identify components of the calcium/calmodulin cascade required for movement of quiescent cells into the cell cycle a...
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Recent biochemical studies of p190, a calmodulin (CM)-binding protein purified from vertebrate brain, have demonstrated that this protein, purified as a complex with bound CM, shares a number of properties with myosins (Espindola, F. S., E. M. Espreafico, M. V. Coelho, A. R. Martins, F. R. C. Costa, M. S. Mooseker, and R. E. Larson. 1992. J. Cell Biol. 118:359-368). To determine whether or not p190 was a member of the myosin family of proteins, a set of overlapping cDNAs encoding the full-length protein sequence of chicken brain p190 was isolated and sequenced. Verification that the deduced primary structure was that of p190 was demonstrated through microsequence analysis of a cyanogen bromide peptide generated from chick brain p190. The deduced primary structure of chicken brain p190 revealed that this 1,830-amino acid (aa) 212,509-D) protein is a member of a novel structural class of unconventional myosins that includes the gene products encoded by the dilute locus of mouse and the MYO2 gene ...
Background: Activation of the protein phosphatase calcineurin is a fundamental signaling event promoting hypertrophic growth and pathological remodeling of the heart. The modulatory calcineurin-interacting protein 1 (MCIP1) is an endogenous feedback inhibitor of calcineurin. We have previously shown that increasing the level of MCIP1 protein protects the heart from unrestrained activation of calcineurin, suggesting that strategies to increase MCIP1 protein in the heart may be a viable therapeutic approach. To this end we have undertaken genetic and biochemical studies to decipher mechanisms that regulate degradation of MCIP1 proteins.. Methods and Results: There are two major isoforms of MCIP1 (MCIP1.1 and MCIP1.4). MCIP1.1 levels are extremely stable with a half-life of over 8 hours in cultured cardiomyocytes. In contrast, MCIP1.4 levels are very low in an unstressed heart but increase precipitously in response to stress and calcineurin activation. Unlike MCIP1.1, the MCIP1.4 protein is ...
The goal of these experiments was to measure the activity of calcineurin in the PBMC of patients with SLE. The major results are as follows. Firstly, there is no difference in the calcineurin activities of PBMC in the GCS-free patients with SLE and healthy control subjects. Secondly, as far as we know, this is the first experimental evidence of decreased calcineurin activity of PBMC induced by GCS used for the treatment of patients with SLE.. Unfractionated PBMC-that is, suspensions of T and B lymphocytes, NK cells, and monocytes, were used in this study because the calcineurin activity assays required a large number of cells. As patients with SLE are lymphopenic it would have been difficult to obtain a sufficient volume of blood for the separation of various purified subsets of the cells. As the subsets of PBMC were characterised by flow cytometry, and as it was found that 69.4% of the cells were T cells, these preparations could be regarded as "T cell-rich suspensions". There was no ...
This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to test code PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.. The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.. Interpretation of this test should be performed in the context of the patients clinical history and other diagnostic tests by a ...
Place all ingredients in slow cooker. Turn on high until it starts bubbling, set to low and let it cook 6 to 8 hours or until chicken gizzards are tender. If you dont like mushy vegetables, add them about 20 to 30 minutes before cooking time is done. Thats it. Enjoy... ...
We have identified at least four loci in distal 11q that when deleted individually or in combination, cause intellectual disability. Deletion of locus III is associated with autism. BSX: Brain-Specific Homeobox protein; Ngn: Neurogranin; RICS: RhoGAP involved in the β-catenin-N-cadherin and NMDA receptor signaling. ...
Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity ...
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Roles of calmodulin and CaMKII in mediating PKG stimulation of Kir6.2/SUR2A channels.Recombinant Kir6.2/SUR2A channels were expressed in HEK293 cells by transie
{loadposition article-preamble} Note: This article assumes you have a working knowledge of EFT. Newcomers can still learn from it but are advised to peruse our Free Gold Standard (Official) EFT Tutorial™ for a more complete understanding. Hi Everyone...

Navigieren Sie durch unsere Neurogranin ELISA KitsNavigieren Sie durch unsere Neurogranin ELISA Kits

... protein kinase C substrate , ng , protein kinase C substrate 7.5 kDa protein , calmodulin-binding protein , neurogranin , B-50 ... This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene ... calcium dynamics are altered in cortical neurons lacking the calmodulin-binding protein RC3 ... Attenuation of protein kinase C (zeige PKC ELISA Kits) and cAMP-dependent protein kinase (zeige CDK7 ELISA Kits) signal ...
more infohttp://www.antikoerper-online.de/abstract/Neurogranin+

Proteomics Analysis of the Expression of Neurogranin in Murine Neuroblastoma (Neuro-2a) Cells Reveals Its Involvement for Cell...Proteomics Analysis of the Expression of Neurogranin in Murine Neuroblastoma (Neuro-2a) Cells Reveals Its Involvement for Cell...

Neurogranin (Ng) is a neural-specific, calmodulin (CaM)-binding protein that is phosphorylated by protein kinase C (PKC). ... Neurogranin (Ng) is among the most abundant proteins known to bind calmodulin (CaM) in a Ca2+-sensitive way in the brain [1]. ... All these proteins share a conserved IQ domain which is homologous to the CaM-binding domains of several other proteins. Ng is ... that corresponds to the protein kinase C phosphorylation site and the calmodulin-binding domain. J Biol Chem. 1991;266:229-237 ...
more infohttp://www.ijbs.com/v03p0263.htm

Pull-down of Calmodulin-binding Proteins | ProtocolPull-down of Calmodulin-binding Proteins | Protocol

... pull-down assay is an effective way to investigate the interaction of CaM with various proteins. This method uses... ... While most target proteins bind Ca2+-CaM, certain proteins only bind to ApoCaM. Some bind CaM through their IQ-domain, ... Add protein loading buffer to all samples (i.e. homogenate, unbound and bound protein as well as those still bound to the beads ... Neurogranin (Ng) represents proteins that bind apo-CaM and CaMKII represents proteins that bind to Ca2+-rich CaM. CaM is shown ...
more infohttps://www.jove.com/video/3502/pull-down-of-calmodulin-binding-proteins

WikiGenes - STRN - striatin, calmodulin binding proteinWikiGenes - STRN - striatin, calmodulin binding protein

A novel calmodulin-binding protein, belonging to the WD-repeat family, is localized in dendrites of a subset of CNS neurons. ... Striatin, a calmodulin-dependent scaffolding protein, directly binds caveolin-1. Gaillard, S., Bartoli, M., Castets, F., ... The polymerase (L) protein of rinderpest virus interacts with the host cell protein striatin. Sleeman, K., Baron, M.D. Virology ... Hence, it is likely that the proteins of the striatin family are addressed to membrane microdomains by their binding to ...
more infohttps://www.wikigenes.org/e/gene/e/6801.html

Pull-down of Calmodulin-binding Proteins | Protocol (Translated to Danish)Pull-down of Calmodulin-binding Proteins | Protocol (Translated to Danish)

Calmodulin (CAM) pull-down-analysen er en effektiv måde at undersøge samspillet mellem Cam med forskellige proteiner. Denne ... Kaleka, K. S., Petersen, A. N., Florence, M. A., Gerges, N. Z. Pull-down of Calmodulin-binding Proteins. J. Vis. Exp. (59), ... Hathaway, D. R., Adelstein, R. S. Human platelet myosin light chain kinase requires the calcium-binding protein calmodulin for ... Identification and characterization of the calmodulin-binding domain of neuromodulin, a neurospecific calmodulin-binding ...
more infohttps://www.jove.com/video/3502/pull-down-af-calmodulin-bindende-proteiner?language=Danish

Calmodulin-binding proteins - WikipediaCalmodulin-binding proteins - Wikipedia

Calmodulin-binding proteins are, as their name implies, proteins which bind calmodulin. Examples include: Gap-43 protein ( ... Caldesmon Calmodulin-Binding Proteins at the US National Library of Medicine Medical Subject Headings (MeSH). ...
more infohttps://en.wikipedia.org/wiki/Calmodulin-binding_proteins

The calmodulin-binding protein IQM1 interacts with CATALASE2 to affect pathogen defense | Plant PhysiologyThe calmodulin-binding protein IQM1 interacts with CATALASE2 to affect pathogen defense | Plant Physiology

Calmodulin (CaM) regulates plant disease responses through its downstream calmodulin-binding proteins (CaMBPs) often by ... The calmodulin-binding protein IQM1 interacts with CATALASE2 to affect pathogen defense. Tianxiao Lv, Xiaoming Li, Tian Fan, ... The calmodulin-binding protein IQM1 interacts with CATALASE2 to affect pathogen defense ... IQ-Motif Containing Protein 1), a Ca2+-independent CaMBP, in JA biosynthesis and defense against the necrotrophic pathogen ...
more infohttp://www.plantphysiol.org/content/early/2019/09/23/pp.19.01060

Neurogranin precursor, Calmodulin binding protein, HRGN or NEUG AntibodyNeurogranin precursor, Calmodulin binding protein, HRGN or NEUG Antibody

Neurogranin is a calmodulin-binding protein expressed exclusively in the brain, particularly in dendritic spines, and ... participating in the protein kinase C signaling pathway. Designed for immunohistochemistry, western blotting and related ... Neurogranin is a calmodulin-binding protein expressed exclusively in the brain, particularly in dendritic spines, and ... Neurogranin is the main postsynaptic protein regulating the availability of calmodulin, binding to it in the absence of calcium ...
more infohttps://www.neuromics.com/GT41024

Conserved Microsynteny of NPR1 with Genes Encoding a Signal Calmodulin-Binding Protein and a CK1-Class Protein Kinase in Beta...Conserved Microsynteny of NPR1 with Genes Encoding a Signal Calmodulin-Binding Protein and a CK1-Class Protein Kinase in Beta...

Conserved Microsynteny of NPR1 with Genes Encoding a Signal Calmodulin-Binding Protein and a CK1-Class Protein Kinase in Beta ... "Conserved Microsynteny of NPR1 with Genes Encoding a Signal Calmodulin-Binding Protein and a CK1-Class Protein Kinase in Beta ...
more infohttps://www.hindawi.com/journals/ijpg/2008/391259/cta/

Conserved Microsynteny of NPR1 with Genes Encoding a Signal Calmodulin-Binding Protein and a CK1-Class Protein Kinase in Beta...Conserved Microsynteny of NPR1 with Genes Encoding a Signal Calmodulin-Binding Protein and a CK1-Class Protein Kinase in Beta...

Conserved Microsynteny of NPR1 with Genes Encoding a Signal Calmodulin-Binding Protein and a CK1-Class Protein Kinase in Beta ... encoding a chloroplast-targeted signal calmodulin-binding protein, and CK1PK, a CK1-class protein kinase. Conserved ... In this study, systematic DNA sequence analysis, gene annotation, and protein BLASTs were performed to determine genes near the ...
more infohttps://www.hindawi.com/journals/ijpg/2008/391259/abs/

Calmodulin from the Eucestoda and its use as a probe for calmodulin binding proteins | Biochemical Society TransactionsCalmodulin from the Eucestoda and its use as a probe for calmodulin binding proteins | Biochemical Society Transactions

Calmodulin from the Eucestoda and its use as a probe for calmodulin binding proteins. JANE L. BROOK, C. BRANFORD-WHITE, W.J. ... Calmodulin from the Eucestoda and its use as a probe for calmodulin binding proteins ... Calmodulin from the Eucestoda and its use as a probe for calmodulin binding proteins ... Calmodulin from the Eucestoda and its use as a probe for calmodulin binding proteins ...
more infohttp://www.biochemsoctrans.org/content/20/3/300S

CALMODULIN AND CALMODULIN-BINDING PROTEINS IN PLANTS, Annual Review of Plant Biology | 10.1146/annurev.arplant.49.1.697 |...CALMODULIN AND CALMODULIN-BINDING PROTEINS IN PLANTS, Annual Review of Plant Biology | 10.1146/annurev.arplant.49.1.697 |...

"CALMODULIN AND CALMODULIN-BINDING PROTEINS IN PLANTS, Annual Review of Plant Biology" on DeepDyve, the largest online rental ... CALMODULIN AND CALMODULIN-BINDING PROTEINS IN PLANTS. CALMODULIN AND CALMODULIN-BINDING PROTEINS IN PLANTS Zielinski, Raymond E ... CALMODULIN AND CALMODULIN-BINDING PROTEINS IN PLANTS. Zielinski, Raymond E. Annual Review of Plant Biology. , Volume 49 (1) - ... Zielinski, R. (1998). CALMODULIN AND CALMODULIN-BINDING PROTEINS IN PLANTS. Annual Review of Plant Biology, 49(1), 697-725.. ...
more infohttps://www.deepdyve.com/lp/annual-reviews/calmodulin-and-calmodulin-binding-proteins-in-plants-spmbdCAvfK?impressionId=5d08c4dfe8d2a&i_medium=docview&i_campaign=references&i_source=references

The transient receptor potential, TRP4, cation channel is a novel member of the family of calmodulin binding proteins |...The transient receptor potential, TRP4, cation channel is a novel member of the family of calmodulin binding proteins |...

The transient receptor potential, TRP4, cation channel is a novel member of the family of calmodulin binding proteins. Claudia ... The transient receptor potential, TRP4, cation channel is a novel member of the family of calmodulin binding proteins ... The transient receptor potential, TRP4, cation channel is a novel member of the family of calmodulin binding proteins ... The transient receptor potential, TRP4, cation channel is a novel member of the family of calmodulin binding proteins ...
more infohttp://www.biochemj.org/content/355/3/663

A kinesin-like calmodulin-binding protein in Chlamydomonas: evidence for a role in cell division and flagellar functions |...A kinesin-like calmodulin-binding protein in Chlamydomonas: evidence for a role in cell division and flagellar functions |...

This family includes a unique kinesin with a calmodulin-binding domain referred to as kinesin-like calmodulin-binding protein ( ... Wargo et al., 2005)] to detect calmodulin binding. However, in both cases CrKCBP does not bind to calmodulin in either high or ... And finally, these differences in calmodulin binding may be due to the variability of the calmodulin-binding domain of CrKCBP ... calmodulin binding of CrKCBP or that our preparation of CrKCBP contains flagellar proteins that inhibit calmodulin binding. ...
more infohttp://jcs.biologists.org/content/119/15/3107

Cellular regulators, Part A, Calcium- and calmodulin-binding proteins - Boston University LibrariesCellular regulators, Part A, Calcium- and calmodulin-binding proteins - Boston University Libraries

... and calmodulin-binding proteins. Title Cellular regulators. Title number Part A. Title part Calcium- and calmodulin-binding ... A. Calcum- and calmodulin-binding proteins -- pt. B. Calcum and lipids. Dimensions 24 cm.. Extent xxxi, 917 pages. Isbn ... A. Calcum- and calmodulin-binding proteins -- pt. B. Calcum and lipids. Dimensions 24 cm.. Extent xxxi, 917 pages. Isbn ... The item Cellular regulators, Part A, Calcium- and calmodulin-binding proteins, edited by Anthony R. Means, P. Michael Conn ...
more infohttp://link.bu.edu/portal/Cellular-regulators-Part-A-Calcium--and/VB6vzCTzZ3U/

CALCIUM/CALMODULIN-BINDING PROTEINS AND THEIR ROLE IN REPRODUCTIVE DEVELOPMENT AND STRESS RESPONSES IN PLANTSCALCIUM/CALMODULIN-BINDING PROTEINS AND THEIR ROLE IN REPRODUCTIVE DEVELOPMENT AND STRESS RESPONSES IN PLANTS

... ... In recent years, a variety of calcium/calmodulin-binding proteins with unique regulatory controls have been identified that ... Herein, for the first time two plant calcium/calmodulin-binding transcription factors, GT2-like protein (AtGT2L) and ... It has been well documented that calcium/calmodulin signaling plays important roles in plant growth, development and responses ...
more infohttps://research.wsulibs.wsu.edu/xmlui/handle/2376/3533

Degradation of PEP-19, a calmodulin-binding protein, by calpain is implicated in neuronal cell death induced by intracellular...Degradation of PEP-19, a calmodulin-binding protein, by calpain is implicated in neuronal cell death induced by intracellular...

Kanazawa Y, Makino M, Morishima Y, Yamada K, Nabeshima T, Shirasaki Y. Degradation of PEP-19, a calmodulin-binding protein, by ... Degradation of PEP-19, a calmodulin-binding protein, by calpain is implicated in neuronal cell death induced by intracellular ... title = "Degradation of PEP-19, a calmodulin-binding protein, by calpain is implicated in neuronal cell death induced by ... Degradation of PEP-19, a calmodulin-binding protein, by calpain is implicated in neuronal cell death induced by intracellular ...
more infohttps://pure.fujita-hu.ac.jp/en/publications/degradation-of-pep-19-a-calmodulin-binding-protein-by-calpain-is-

PLOS Computational Biology: Effect of Ca2+ on the promiscuous target-protein binding of calmodulinPLOS Computational Biology: Effect of Ca2+ on the promiscuous target-protein binding of calmodulin

One domain of calmodulin is shown to be selective with binding involving short-distance interactions between hydrophobic ... The mechanisms behind the simultaneous specificity and variation of target protein binding is yet unknown but will aid ... while the other binds target proteins through a more flexible mechanism involving long-distance electrostatic interactions. ... Its two lobes bind calcium and allow the energetically unfavorable exposure of hydrophobic residues to the aqueous environment ...
more infohttps://journals.plos.org/ploscompbiol/article/figure?id=10.1371/journal.pcbi.1006072.g006

Multiple roles of calmodulin and other Ca(2+)-binding proteins in the functional regulation of TRP channels.  - PubMed - NCBIMultiple roles of calmodulin and other Ca(2+)-binding proteins in the functional regulation of TRP channels. - PubMed - NCBI

... binding proteins. In vitro binding studies have revealed multiple CaM-binding sites on TRPC proteins. Among them, a common CaM/ ... Multiple roles of calmodulin and other Ca(2+)-binding proteins in the functional regulation of TRP channels.. Zhu MX1. ... Likewise, multiple CaM-binding sites were found in other TRP proteins. These, together with the presence in close vicinity of ... Additional non-conserved CaM-binding sites are present at the amino and carboxyl termini of several TRPC proteins. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/15924238

Impaired Synaptic Plasticity and cAMP Response Element-Binding Protein Activation in Ca2+/Calmodulin-Dependent Protein Kinase...Impaired Synaptic Plasticity and cAMP Response Element-Binding Protein Activation in Ca2+/Calmodulin-Dependent Protein Kinase...

1991) A novel Ca2+/calmodulin-dependent protein kinase and a male germ cell-specific calmodulin-binding protein are derived ... Impaired Synaptic Plasticity and cAMP Response Element-Binding Protein Activation in Ca2+/Calmodulin-Dependent Protein Kinase ... Impaired Synaptic Plasticity and cAMP Response Element-Binding Protein Activation in Ca2+/Calmodulin-Dependent Protein Kinase ... Impaired Synaptic Plasticity and cAMP Response Element-Binding Protein Activation in Ca2+/Calmodulin-Dependent Protein Kinase ...
more infohttps://www.jneurosci.org/content/20/17/6459.long

Calmodulin binding distinguishes between βγ subunits of activated G proteins and transducin | Biochemical JournalCalmodulin binding distinguishes between βγ subunits of activated G proteins and transducin | Biochemical Journal

The interactions between guanine nucleotide regulatory proteins and the Ca(2+)-binding protein calmodulin were studied using ... Calmodulin binding distinguishes between βγ subunits of activated G proteins and transducin. L A Mangels, R R Neubig, H E Hamm ... Calmodulin binding distinguishes between βγ subunits of activated G proteins and transducin ... Calmodulin binding distinguishes between βγ subunits of activated G proteins and transducin ...
more infohttp://www.biochemj.org/content/283/3/683

The 58-Kilodalton Calmodulin-Binding Glutamate Decarboxylase Is a Ubiquitous Protein in Petunia Organs and Its Expression Is...The 58-Kilodalton Calmodulin-Binding Glutamate Decarboxylase Is a Ubiquitous Protein in Petunia Organs and Its Expression Is...

The 58-Kilodalton Calmodulin-Binding Glutamate Decarboxylase Is a Ubiquitous Protein in Petunia Organs and Its Expression Is ... The 58-Kilodalton Calmodulin-Binding Glutamate Decarboxylase Is a Ubiquitous Protein in Petunia Organs and Its Expression Is ... The 58-Kilodalton Calmodulin-Binding Glutamate Decarboxylase Is a Ubiquitous Protein in Petunia Organs and Its Expression Is ... The 58-Kilodalton Calmodulin-Binding Glutamate Decarboxylase Is a Ubiquitous Protein in Petunia Organs and Its Expression Is ...
more infohttp://www.plantphysiol.org/content/106/4/1381

Calmodulin Binding to the Polybasic C-Termini of STIM Proteins Involved in Store-Operated Calcium Entry.Calmodulin Binding to the Polybasic C-Termini of STIM Proteins Involved in Store-Operated Calcium Entry.

... Bauer, Mikael LU ; ... By binding STIM1 and STIM2, calmodulin may regulate store refilling, thereby ensuring the maintenance of its own action in ... By binding STIM1 and STIM2, calmodulin may regulate store refilling, thereby ensuring the maintenance of its own action in ... We show by isothermal titration calorimetry that calmodulin binds in a calcium-dependent manner to the polybasic C-termini of ...
more infohttps://lup.lub.lu.se/search/publication/b9d2365a-127e-4594-ba0f-c97b460d2fa1

Ca2+/calmodulin-dependent protein kinase II (CaMKII) is activated by calmodulin with two bound calciums | PNASCa2+/calmodulin-dependent protein kinase II (CaMKII) is activated by calmodulin with two bound calciums | PNAS

Calmodulin (CaM) is a major Ca2+ effector protein that binds up to four Ca2+ ions. CaM with bound Ca2+ can activate at least ... Ca2+/calmodulin-dependent protein kinase II;. CaM,. calmodulin;. CaM-NWT,. mutant CaM that binds Ca2+ only at N-terminal sites; ... Ca2+/calmodulin-dependent protein kinase II (CaMKII) is activated by calmodulin with two bound calciums. Julia M. Shifman, Mee ... Ca2+/calmodulin-dependent protein kinase II (CaMKII) is activated by calmodulin with two bound calciums ...
more infohttps://www.pnas.org/content/103/38/13968?ijkey=d306f3f6070baa20cedd081295787464ede529ec&keytype2=tf_ipsecsha

Multiple Roles of Calmodulin and Other Ca(2+)-binding Proteins in the Functional Regulation of TRP Channels - PubMedMultiple Roles of Calmodulin and Other Ca(2+)-binding Proteins in the Functional Regulation of TRP Channels - PubMed

... binding proteins. In vitro binding studies have revealed multiple CaM-binding sites on TRPC proteins. Among them, a common CaM/ ... Multiple Roles of Calmodulin and Other Ca(2+)-binding Proteins in the Functional Regulation of TRP Channels Michael Xi Zhu 1 ... Multiple Roles of Calmodulin and Other Ca(2+)-binding Proteins in the Functional Regulation of TRP Channels Michael Xi Zhu. ... Proteins With Calmodulin-Like Domains: Structures and Functional Roles A Villalobo et al. Cell Mol Life Sci 76 (12), 2299-2328 ...
more infohttps://pubmed.ncbi.nlm.nih.gov/15924238/
  • Ca 2+ -binding and enzymatic activation properties of calmodulin are discussed emphasizing the functional linkages between these processes and the diverse pathways that are dependent on Ca 2+ signaling. (deepdyve.com)
  • In recent years, a variety of calcium/calmodulin-binding proteins with unique regulatory controls have been identified that play critical roles in these signaling pathways. (wsu.edu)
  • Our results support the idea that competition for binding of CaM with two bound Ca 2+ ions may influence significantly the outcome of local Ca 2+ signaling in spines and, perhaps, in other signaling pathways. (pnas.org)
  • Platelet-surface sheddases, particularly of the metalloproteinase-disintegrin (ADAM) family, can be regulated by many of the same mechanisms that control receptor function, such as calmodulin association or activation of signaling pathways. (ahajournals.org)
  • 2-6 Major consequences of signal transduction following engagement of GPVI or GPIb-IX-V include (1) activation of platelet integrins, primarily α IIb β 3 that binds vWF or fibrinogen and mediates platelet aggregation, and (2) activation of pathways leading to metalloproteinase-dependent receptor ectodomain shedding. (ahajournals.org)
  • Ca 2+ that flows into a spine through activated NMDA receptors binds to CaM and to several other Ca 2+ -binding proteins. (pnas.org)
  • In particular, platelet-specific receptors, glycoprotein (GP)VI and the GPIb-IX-V complex that bind collagen or von Willebrand factor (vWF), respectively, form a physical and functional complex 1 initiating platelet thrombus formation at high shear stress in flowing blood. (ahajournals.org)
  • Synthetic peptides, encompassing the two putative CaM binding sites within these domains and covering amino acid residues 694-728 and 829-853, interacted directly with dansyl-CaM with apparent K d values of 94-189nM. (biochemj.org)
  • To test whether CrKCBP is involved in additional microtubule-based processes not observed in plants, we generated antibodies against the putative calmodulin-binding domain and used these antibodies in biochemical and localization studies. (biologists.org)
  • In this study, systematic DNA sequence analysis, gene annotation, and protein BLASTs were performed to determine genes near the NPR1 gene in Beta vulgaris L., Medicago truncatula Gaertn, and Populus trichocarpa Torr. (hindawi.com)
  • The mammalian gene products, transient receptor potential (trp)1 to trp7, are related to the Drosophila TRP and TRP-like ion channels, and are candidate proteins underlying agonist-activated Ca 2+ -permeable ion channels. (biochemj.org)
  • CaMKIV/Gr-deficient mice exhibited impaired neuronal cAMP-responsive element binding protein (CREB) phosphorylation and Ca 2+ /CREB-dependent gene expression. (jneurosci.org)
  • Caldesmon is a protein that in humans is encoded by the CALD1 gene. (wikipedia.org)
  • This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. (wikipedia.org)
  • Among them, a common CaM/inositol 1,4,5-trisphosphate receptor-binding site is found at the carboxyl terminus of every TRPC isoform. (nih.gov)
  • In addition, production of chimeric kinesins containing the calmodulin-binding domain have shown that this domain can act as a regulatory module in animal chimeric kinesins including N-kinesins that are plus-end-directed motors ( Reddy and Reddy, 2002 ). (biologists.org)
  • CaM mediates a host of regulatory effects of Ca 2+ despite the fact that the Ca 2+ concentration in the cytosol rarely reaches a level expected to saturate all four Ca 2+ -binding sites on free CaM ( 1 ). (pnas.org)
  • In immunoprecipitation assay, wild type PEP-19 associated with calmodulin, whereas mutated PEP-19, which contains mutations within the calmodulin binding site of PEP-19, failed to associate with calmodulin. (fujita-hu.ac.jp)
  • To study CaM-dependent signaling in the spine, we designed mutant CaMs that bind Ca 2+ only at the two N-terminal or two C-terminal sites by using computationally designed mutations to stabilize the inactivated Ca 2+ -binding domains in the "closed" Ca 2+ -free conformation. (pnas.org)
  • Because of this property, we predict that when Ca 2+ concentrations fluctuate in the cytosol, competition among targets for binding of CaM will depend on their affinities for CaM with one or two bound Ca 2+ and also on the magnitude of cooperativity induced by binding to each target. (pnas.org)
  • Binding to targets increases the affinity of CaM's remaining Ca 2+ -binding sites. (pnas.org)
  • Thus, initial binding of CaM to a target may depend on the target's affinity for CaM with only one or two bound Ca 2+ ions. (pnas.org)
  • Each lobe contains two EF hands that bind Ca 2+ with low micromolar affinity. (pnas.org)
  • This sensitivity is possible because association of CaM with its targets stabilizes its Ca 2+ -bound conformation and, therefore, increases its affinity for Ca 2+ ( 2 - 4 ). (pnas.org)
  • When CaM with two Ca 2+ ions bound to either the N- or the C-terminal lobe associates with a target, the affinity of the remaining lobe for Ca 2+ increases, often by at least one order of magnitude, causing high positive cooperativity of Ca 2+ binding ( 5 ). (pnas.org)
  • It has been well documented that calcium/calmodulin signaling plays important roles in plant growth, development and responses to environmental stimuli. (wsu.edu)
  • On the contrary, beta gamma subunits produced in an activated Go/Gi preparation did not bind to calmodulin-Sepharose. (biochemj.org)
  • To distinguish whether the binding of purified beta gamma subunits to calmodulin was unique to brain beta gamma or to the method of purification, similar experiments were performed using transducin. (biochemj.org)
  • We conclude that the 58-kD CaM-binding GAD is a ubiquitous protein in petunia organs and that its expression is developmentally regulated by transcriptional and/or posttranscriptional processes. (plantphysiol.org)
  • p>An evidence describes the source of an annotation, e.g. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc. (uniprot.org)
  • be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein. (uniprot.org)
  • However, unactivated Go/Gi and preactivated Go/Gi inhibited calmodulin-stimulated adenylate cyclase activity to the same extent. (biochemj.org)
  • Recently, the TRP4 protein has been shown to be part of native store-operated Ca 2+ -permeable channels. (biochemj.org)
  • These channels, most likely, are composed of other proteins in addition to TRP4. (biochemj.org)
  • Purified beta gamma was highly effective in inhibiting calmodulin-stimulated adenylate cyclase activity. (biochemj.org)
  • We found that the mutation abrogates both the ability to suppress calmodulin-dependent signaling and to protect cells from death. (fujita-hu.ac.jp)
  • The item Cellular regulators, Part A, Calcium- and calmodulin-binding proteins, edited by Anthony R. Means, P. Michael Conn represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in Boston University Libraries . (bu.edu)