Proteins which bind calmodulin. They are found in many tissues and have a variety of functions including F-actin cross-linking properties, inhibition of cyclic nucleotide phosphodiesterase and calcium and magnesium ATPases.
A heat-stable, low-molecular-weight activator protein found mainly in the brain and heart. The binding of calcium ions to this protein allows this protein to bind to cyclic nucleotide phosphodiesterases and to adenyl cyclase with subsequent activation. Thereby this protein modulates cyclic AMP and cyclic GMP levels.
A phenothiazine with actions similar to CHLORPROMAZINE. It is used as an antipsychotic and an antiemetic.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
An enzyme that phosphorylates myosin light chains in the presence of ATP to yield myosin-light chain phosphate and ADP, and requires calcium and CALMODULIN. The 20-kDa light chain is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors. The enzyme plays a central role in the regulation of smooth muscle contraction.
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
A nervous tissue specific protein which is highly expressed in NEURONS during development and NERVE REGENERATION. It has been implicated in neurite outgrowth, long-term potentiation, SIGNAL TRANSDUCTION, and NEUROTRANSMITTER release. (From Neurotoxicology 1994;15(1):41-7) It is also a substrate of PROTEIN KINASE C.
The rate dynamics in chemical or physical systems.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A subclass of myosins found generally associated with actin-rich membrane structures such as filopodia. Members of the myosin type I family are ubiquitously expressed in eukaryotes. The heavy chains of myosin type I lack coiled-coil forming sequences in their tails and therefore do not dimerize.
A BRAIN-specific substrate for PROTEIN KINASE C that binds CALMODULIN and is involved in regulation of CALCIUM SIGNALING.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Cation-transporting proteins that utilize the energy of ATP hydrolysis for the transport of CALCIUM. They differ from CALCIUM CHANNELS which allow calcium to pass through a membrane without the use of energy.
Proteins that bind to the 3' polyadenylated region of MRNA. When complexed with RNA the proteins serve an array of functions such as stabilizing the 3' end of RNA, promoting poly(A) synthesis and stimulating mRNA translation.
The profession of writing. Also the identity of the writer as the creator of a literary production.
Collections of facts, assumptions, beliefs, and heuristics that are used in combination with databases to achieve desired results, such as a diagnosis, an interpretation, or a solution to a problem (From McGraw Hill Dictionary of Scientific and Technical Terms, 6th ed).
A synthetic pregnadiene derivative with anti-aldosterone activity.
The guidelines and policy statements set forth by the editor(s) or editorial board of a publication.
Intentional falsification of scientific data by presentation of fraudulent or incomplete or uncorroborated findings as scientific fact.
A heterotrimeric GTP-binding protein that mediates the light activation signal from photolyzed rhodopsin to cyclic GMP phosphodiesterase and is pivotal in the visual excitation process. Activation of rhodopsin on the outer membrane of rod and cone cells causes GTP to bind to transducin followed by dissociation of the alpha subunit-GTP complex from the beta/gamma subunits of transducin. The alpha subunit-GTP complex activates the cyclic GMP phosphodiesterase which catalyzes the hydrolysis of cyclic GMP to 5'-GMP. This leads to closure of the sodium and calcium channels and therefore hyperpolarization of the rod cells. EC 3.6.1.-.
Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.
Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.
Short-term debt obligations and assets occurring in the regular course of operational transactions.
Public Law 104-91 enacted in 1996, was designed to improve the efficiency and effectiveness of the healthcare system, protect health insurance coverage for workers and their families, and to protect individual personal health information.
A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
A microtubule-associated mechanical adenosine triphosphatase, that uses the energy of ATP hydrolysis to move organelles along microtubules toward the plus end of the microtubule. The protein is found in squid axoplasm, optic lobes, and in bovine brain. Bovine kinesin is a heterotetramer composed of two heavy (120 kDa) and two light (62 kDa) chains. EC 3.6.1.-.
A family of multisubunit cytoskeletal motor proteins that use the energy of ATP hydrolysis to power a variety of cellular functions. Dyneins fall into two major classes based upon structural and functional criteria.
A genus GREEN ALGAE in the order VOLVOCIDA. It consists of solitary biflagellated organisms common in fresh water and damp soil.
A species of GREEN ALGAE. Delicate, hairlike appendages arise from the flagellar surface in these organisms.
Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.
Protective measures against unauthorized access to or interference with computer operating systems, telecommunications, or data structures, especially the modification, deletion, destruction, or release of data in computers. It includes methods of forestalling interference by computer viruses or so-called computer hackers aiming to compromise stored data.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
The privacy of information and its protection against unauthorized disclosure.
The state of being free from intrusion or disturbance in one's private life or affairs. (Random House Unabridged Dictionary, 2d ed, 1993)
A short thick vein formed by union of the superior mesenteric vein and the splenic vein.
Sequential operating programs and data which instruct the functioning of a digital computer.
Positively charged atoms, radicals or groups of atoms which travel to the cathode or negative pole during electrolysis.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
The cell center, consisting of a pair of CENTRIOLES surrounded by a cloud of amorphous material called the pericentriolar region. During interphase, the centrosome nucleates microtubule outgrowth. The centrosome duplicates and, during mitosis, separates to form the two poles of the mitotic spindle (MITOTIC SPINDLE APPARATUS).
Common name for two distinct groups of BIRDS in the order GALLIFORMES: the New World or American quails of the family Odontophoridae and the Old World quails in the genus COTURNIX, family Phasianidae.
Nerve fibers that are capable of rapidly conducting impulses away from the neuron cell body.
A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.
The process whereby a representation of past experience is elicited.
The knowledge or perception that someone or something present has been previously encountered.
Occupations of medical personnel who are not physicians, and are qualified by special training and, frequently, by licensure to work in supporting roles in the health care field. These occupations include, but are not limited to, medical technology, physical therapy, physician assistant, etc.
The process of discovering or asserting an objective or intrinsic relation between two objects or concepts; a faculty or power that enables a person to make judgments; the process of bringing to light and asserting the implicit meaning of a concept; a critical evaluation of a person or situation.
Books used in the study of a subject that contain a systematic presentation of the principles and vocabulary of a subject.
A subjective feeling that an experience which is occurring for the first time has been experienced before.

Adducin polymorphism affects renal proximal tubule reabsorption in hypertension. (1/1442)

Abnormalities in renal sodium reabsorption may be involved in the development and maintenance of experimental and clinical hypertension. Adducin polymorphism is thought to regulate ion transport in the renal tubule. It has recently been shown that there is a significant linkage of alpha-adducin locus to essential hypertension and that the 460Trp allele is associated with hypertension. Patients with this allele display larger blood pressure changes with body sodium variation. The aim of this study was to test whether alpha-adducin polymorphism is involved in abnormalities of renal function. Because proximal tubular reabsorption has been shown to be tightly coupled to renal perfusion pressure, this segmental tubular function was investigated in 54 (29 Gly/Gly and 25 Gly/Trp) untreated hypertensive patients in basal conditions with the use of endogenous lithium concentration and uric acid. Fractional excretions of lithium and uric acid were significantly decreased in the Gly/Trp hypertensive patients compared with the Gly/Gly hypertensives. The contribution of alpha-adducin to fractional excretion of lithium was investigated by multiple regression analysis. Adducin genotype was significantly (R2=0.11, F=6.5; P<0.01) and directly related to fraction excretion of lithium; gender, age, urinary Na+, urinary uric acid, mean blood pressure, and plasma renin activity were not related. In conclusion, the adducin gene can be considered to be a 'renal hypertensive gene' that modulates the capacity of tubular epithelial cells to transport Na+ and hence contributes to the level of blood pressure.  (+info)

PST 2238: A new antihypertensive compound that modulates Na,K-ATPase in genetic hypertension. (2/1442)

A genetic alteration in the adducin genes is associated with hypertension and up-regulation of the expression of renal Na, K-ATPase in Milan-hypertensive (MHS) rats, in which increased ouabain-like factor (OLF) levels are also observed. PST 2238, a new antihypertensive compound that antagonizes the pressor effect of ouabain in vivo and normalizes ouabain-dependent up-regulation of the renal Na-K pump, was evaluated for its ability to lower blood pressure and regulate renal Na,K-ATPase activity in MHS genetic hypertension. In this study, we show that PST 2238, given orally at very low doses (1 and 10 microg/kg for 5-6 weeks), reduced the development of hypertension in MHS rats and normalized the increased renal Na,K-ATPase activity and mRNA levels, whereas it did not affect either blood pressure or Na,K-ATPase in Milan-normotensive (MNS) rats. In addition, a similar antihypertensive effect was observed in adult MHS rats after a short-term treatment. In cultured rat renal cells with increased Na-K pump activity at Vmax due to overexpression of the hypertensive variant of adducin, 5 days of incubation with PST 2238 (10(-10-)-10(-9) M) lowered the pump rate to the level of normal wild-type cells, which in turn were not affected by the drug. In conclusion, PST 2238 is a very potent compound that in MHS rats reduces blood pressure and normalizes Na-K pump alterations caused by a genetic alteration of the cytoskeletal adducin. Because adducin gene mutations have been associated with human essential hypertension, it is suggested that PST 2238 may display greater antihypertensive activity in those patients carrying such a genetic alteration.  (+info)

Transcriptional down-regulation of the rabbit pulmonary artery endothelin B receptor during phenotypic modulation. (3/1442)

1. We confirmed that endothelium-independent contraction of the rabbit pulmonary artery (RPA) is mediated through both an endothelin A (ET(A)R) and endothelin B (ET(B2)R) receptor. 2. The response of endothelium-denuded RPA rings to endothelin-1 (ET-1, pD2 = 7.84 +/- 0.03) was only partially inhibited by BQ123 (10 microM), an ET(A)R antagonist. 3. Pretreatment with 1 nM sarafotoxin S6c (S6c), an ET(B)R agonist, desensitized the ET(B2)R and significantly attenuated the response to ET-3 (pD2 = 7.40 +/- 0.02 before, <6.50 after S6c). 4. Pretreatment with S6c had little effect on the response to ET-1, but BQ123 (10 microM) caused a parallel shift to the right of the residual ETAR-mediated response to ET-1 (pD2 = 7.84 +/- 0.03 before S6c, 7.93 +/- 0.03 after S6c, 6.81 +/- 0.05 after BQ123). 5. Binding of radiolabelled ET-1 to early passage cultures of RPA vascular smooth muscle cells (VSMC) displayed two patterns of competitive displacement characteristic of the ET(A)R (BQ123 pIC50 = 8.73 +/- 0.05) or ET(B2)R (S6c pIC50 = 10.15). 6. Competitive displacement experiments using membranes from late passage VSMC confirmed only the presence of the ET(A)R (ET-1 pIC50 = 9.3, BQ123 pIC50 = 8.0, S6c pIC50 < 6.0). 7. The ET(A)R was functionally active and coupled to rises in intracellular calcium which exhibited prolonged homologous desensitization. 8. Using a reverse transcriptase polymerase chain reaction for the rabbit ET(B2)R, we demonstrated the absence of mRNA expression in phenotypically modified VSMC. 9. We conclude that the ET(B2)R expressed by VSMC which mediates contraction of RPA is rapidly down-regulated at the transcriptional level during phenotypic modulation in vitro.  (+info)

Interactions between neurogranin and calmodulin in vivo. (4/1442)

Neurogranin is a neural-specific, calmodulin (CaM)-binding protein that is phosphorylated by protein kinase C (PKC) within its IQ domain at serine 36. Since CaM binds to neurogranin through the IQ domain, PKC phosphorylation and CaM binding are mutually exclusive. Consequently, we hypothesize that neurogranin may function to concentrate CaM at specific sites in neurons and release free CaM in response to increased Ca2+ and PKC activation. However, it has not been established that neurogranin interacts with CaM in vivo. In this study, we examined this question using yeast two-hybrid methodology. We also searched for additional proteins that might interact with neurogranin by screening brain cDNA libraries. Our data illustrate that CaM binds to neurogranin in vivo and that CaM is the only neurogranin-interacting protein isolated from brain cDNA libraries. Single amino acid mutagenesis indicated that residues within the IQ domain are important for CaM binding to neurogranin in vivo. The Ile-33 --> Gln point mutant completely inhibited and Arg-38 --> Gln and Ser-36 --> Asp point mutants reduced neurogranin/CaM interactions. These data demonstrate that CaM is the major protein that interacts with neurogranin in vivo and support the hypothesis that phosphorylation of neurogranin at Ser-36 regulates its binding to CaM.  (+info)

Mechanical and chemical unfolding of a single protein: a comparison. (5/1442)

Is the mechanical unraveling of protein domains by atomic force microscopy (AFM) just a technological feat or a true measurement of their unfolding? By engineering a protein made of tandem repeats of identical Ig modules, we were able to get explicit AFM data on the unfolding rate of a single protein domain that can be accurately extrapolated to zero force. We compare this with chemical unfolding rates for untethered modules extrapolated to 0 M denaturant. The unfolding rates obtained by the two methods are the same. Furthermore, the transition state for unfolding appears at the same position on the folding pathway when assessed by either method. These results indicate that mechanical unfolding of a single protein by AFM does indeed reflect the same event that is observed in traditional unfolding experiments. The way is now open for the extensive use of AFM to measure folding reactions at the single-molecule level. Single-molecule AFM recordings have the added advantage that they define the reaction coordinate and expose rare unfolding events that cannot be observed in the absence of chemical denaturants.  (+info)

Identification of chURP, a nuclear calmodulin-binding protein related to hnRNP-U. (6/1442)

In a screen for myosin-like proteins in embryonic chicken brain, we have identified a novel nuclear protein structurally related to hnRNP-U (heterogeneous nuclear ribonuclear protein U). We have called this protein chURP, for chicken U-related protein. In this screen, chURP was immunoreactive with two myosin antibodies and, in common with the unconventional myosins, bound calmodulin in vitro in both the presence and absence of calcium ions. Determination of 757 amino acids of the chURP sequence revealed that it shares 41% amino acid identity with human and rat hnRNP-U, although chURP and hnRNP-U appear not to be orthologous proteins. ChURP is ubiquitously expressed in the nuclei of all chick tissues and, as one of a growing number of calmodulin-binding proteins to be identified in the nucleus, further highlights the potential of calmodulin as a regulator of nuclear metabolism.  (+info)

Identification of the calmodulin-binding domain of neuron-specific protein kinase C substrate protein CAP-22/NAP-22. Direct involvement of protein myristoylation in calmodulin-target protein interaction. (7/1442)

Various proteins in the signal transduction pathways as well as those of viral origin have been shown to be myristoylated. Although the modification is often essential for the proper functioning of the modified protein, the mechanism by which the modification exerts its effects is still largely unknown. Brain-specific protein kinase C substrate, CAP-23/NAP-22, which is involved in the synaptogenesis and neuronal plasticity, binds calmodulin, but the protein lacks any canonical calmodulin-binding domain. In the present report, we show that CAP-23/NAP-22 isolated from rat brain is myristoylated and that the modification is directly involved in its interaction with calmodulin. Myristoylated and non-myristoylated recombinant proteins were produced in Escherichia coli, and their calmodulin-binding properties were examined. Only the former bound to calmodulin. Synthetic peptides based on the N-terminal sequence showed similar binding properties to calmodulin, only when they were myristoylated. The calmodulin-binding site narrowed down to the myristoyl moiety together with a nine-amino acid N-terminal basic domain. Phosphorylation of a single serine residue in the N-terminal domain (Ser5) by protein kinase C abolished the binding. Furthermore, phosphorylation of CAP-23/NAP-22 by protein kinase C was also found myristoylation-dependent, suggesting the importance of myristoylation in protein-protein interactions.  (+info)

Phosphorylation of adducin by Rho-kinase plays a crucial role in cell motility. (8/1442)

Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and thereby promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes such as ruffling membranes. Rho-associated kinase (Rho- kinase), which is activated by the small guanosine triphosphatase Rho, phosphorylates alpha-adducin and thereby enhances the F-actin-binding activity of alpha-adducin in vitro. Here we identified the sites of phosphorylation of alpha-adducin by Rho-kinase as Thr445 and Thr480. We prepared antibody that specifically recognized alpha-adducin phosphorylated at Thr445, and found by use of this antibody that Rho-kinase phosphorylated alpha-adducin at Thr445 in COS7 cells in a Rho-dependent manner. Phosphorylated alpha-adducin accumulated in the membrane ruffling area of Madin-Darby canine kidney (MDCK) epithelial cells and the leading edge of scattering cells during the action of tetradecanoylphorbol-13-acetate (TPA) or hepatocyte growth factor (HGF). The microinjection of Botulinum C3 ADP-ribosyl-transferase, dominant negative Rho-kinase, or alpha-adducinT445A,T480A (substitution of Thr445 and Thr480 by Ala) inhibited the TPA-induced membrane ruffling in MDCK cells and wound-induced migration in NRK49F cells. alpha-AdducinT445D,T480D (substitution of Thr445 and Thr480 by Asp), but not alpha-adducinT445A,T480A, counteracted the inhibitory effect of the dominant negative Rho-kinase on the TPA-induced membrane ruffling in MDCK cells. Taken together, these results indicate that Rho-kinase phosphorylates alpha-adducin downstream of Rho in vivo, and that the phosphorylation of adducin by Rho-kinase plays a crucial role in the regulation of membrane ruffling and cell motility.  (+info)

Abstract. Caldesmon, an inhibitory actin binding protein, binds to actin and inhibits actin-myosin interactions, whereas caldesmon phosphorylation reverses the inhibitory effect of caldesmon on actin-myosin interactions, potentially leading to enhanced contraction. The goal of this study was to investigate the cellular signaling pathway responsible for caldesmon phosphorylation, which is involved in the regulation of the contraction induced by dexmedetomidine (DMT), an alpha-2 adrenoceptor agonist, in endothelium-denuded rat aortas. SP600125 (a c-Jun NH2-terminal kinase [JNK] inhibitor) dose-response curves were generated in aortas that were pre-contracted with DMT or phorbol 12,13-dibutyrate (PDBu), a protein kinase C (PKC) activator. Dose-response curves to the PKC inhibitor chelerythrine were generated in rat aortas pre-contracted with DMT. The effects of SP600125 and rauwolscine (an alpha-2 adrenoceptor inhibitor) on DMT-induced caldesmon phosphorylation in rat aortic vascular smooth muscle ...
TY - JOUR. T1 - Degradation of PEP-19, a calmodulin-binding protein, by calpain is implicated in neuronal cell death induced by intracellular Ca2+ overload. AU - Kanazawa, Y.. AU - Makino, M.. AU - Morishima, Y.. AU - Yamada, K.. AU - Nabeshima, T.. AU - Shirasaki, Y.. PY - 2008/6/23. Y1 - 2008/6/23. N2 - Excessive elevation of intracellular Ca2+ levels and, subsequently, hyperactivation of Ca2+/calmodulin-dependent processes might play an important role in the pathologic events following cerebral ischemia. PEP-19 is a neuronally expressed polypeptide that acts as an endogenous negative regulator of calmodulin by inhibiting the association of calmodulin with enzymes and other proteins. The aims of the present study were to investigate the effect of PEP-19 overexpression on cell death triggered by Ca2+ overload and how the polypeptide levels are affected by glutamate-induced excitotoxicity and cerebral ischemia. Expression of PEP-19 in HEK293T cells suppressed calmodulin-dependent signaling and ...
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The mammalian gene products, transient receptor potential (trp)1 to trp7, are related to the Drosophila TRP and TRP-like ion channels, and are candidate proteins underlying agonist-activated Ca2+-permeable ion channels. Recently, the TRP4 protein has been shown to be part of native store-operated Ca2+-permeable channels. These channels, most likely, are composed of other proteins in addition to TRP4. In the present paper we report the direct interaction of TRP4 and calmodulin (CaM) by: (1) retention of in vitro translated TRP4 and of TRP4 protein solubilized from bovine adrenal cortex by CaM-Sepharose in the presence of Ca2+, and (2) TRP4-glutathione S-transferase pull-down experiments. Two domains of TRP4, amino acid residues 688-759 and 786-848, were identified as being able to interact with CaM. The binding of CaM to both domains occurred only in the presence of Ca2+ concentrations above 10µM, with half maximal binding occurring at 16.6µM (domain 1) and 27.9µM Ca2+ (domain 2). Synthetic ...
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Caldesmon is an actin-binding protein that is capable of stabilizing actin filaments against actin-severing proteins, inhibiting actomyosin ATPase activity, and inhibiting Arp2/3-mediated actin polymerization in vitro. Caldesmon is a substrate of cdc2 kinase and Erk1/2 MAPK, and phosphorylation by e …
Erythrocyte membrane alterations mirror those of vascular smooth muscle and renal tubular cell membrane. The interaction between adducin and Na-K pump is the most likely biochemical mechanism responsible for the increased tubular Na reabsorption and hypertension in Milan hypertensive strain (MHS) rats. To substantiate this hypothesis in humans, we tested to see if α-adducin Gly460Trp genotype is associated with erythrocyte sodium transport rate in a new cohort of n268 never-treated North Sardinian primary hypertensives. Plasma renin activity and blood pressure response to hydrochlorothiazide were also measured to evaluate the relationship between sodium transport rate and two intermediate phenotypes with a higher degree of genetic complexity. Na-K pump, Na-K-Cl cotransport, and Li-Na countertransport at Vmax were faster (P,0.0001), whereas intracellular Na concentration was lower (P,0.0001) in patients carrying one or two 460Trp alleles. Such behavior was mirrored by opposite changes of ...
Sequence searching (5) revealed that 14 of the 39 calmodulin-binding proteins contain a motif whose consensus is (I/L)QXXK(K/X)GB, where X is any residue and B is a basic residue (Fig. 2B). A related sequence in myosins, IQXXXXKXXXR, has been shown previously to bind calmodulin (18). Thus, we demonstrate that the domain is found in many calmodulin-binding proteins. Presumably the other targets that lack this motif have other calmodulin-binding sequences (10).. In addition to the calmodulin-binding targets, we also identified one protein, Pyc1p, that bound Cy3-labeled streptavidin. Pyc1p encodes a pyruvate carboxylase 1 homolog that contains a highly conserved biotin attachment region (19). Thus, as predicted by its sequence, Pyc1p is biotinylated in vivo. With appropriate detection assays, we expect that proteome chips can identify many types of posttranslational modification of proteins.. To test whether proteome chips could be used to identify activities that might not be accessible by other ...
The effect of Ca2+ and calmodulin on phosphorylation of islet secretory granule proteins was studied. Secretory granules were incubated in a phosphorylation reaction mixture containing [32P]ATP and test reagents. The 32P-labeled proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the 32P content was visualized by autoradiography, and the relative intensities of specific bands were quantitated. When the reaction mixture contained EGTA and no added Ca2+, 32P was incorporated into two proteins with molecular weights of 45,000 and 13,000. When 10−4 M Ca2+ was added without EGTA, two additional proteins (58,000 and 48,000 Mr) were phosphorylated, and the 13,000-Mr protein was absent. The addition of 2.4 μM calmodulin markedly enhanced the phosphorylation of the 58,000- and 48,000-Mr proteins and resulted in the phosphorylation of a major protein whose molecular weight (64,000 Mr) is identical to that of one of the calmodulin binding proteins located on the granule ...
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008 ...
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dyes functional group. See the product information sheets for specific reactive dyes more information.. Coelenterazines and D-luciferin. Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also ...
We identified and analyzed 33 and 29 IQD1-like genes in Arabidopsis thaliana and Oryza sativa, respectively. The encoded IQD proteins contain a plant-specific domain of 67 conserved amino acid residues, referred to as the IQ67 domain, which is characterized by a unique and repetitive arrangement of three different calmodulin recruitment motifs, known as the IQ, 1-5-10, and 1-8-14 motifs. We demonstrated calmodulin binding for IQD20, the smallest IQD protein in Arabidopsis, which consists of a C-terminal IQ67 domain and a short N-terminal extension. A striking feature of IQD proteins is the high isoelectric point (~10.3) and frequency of serine residues (~11%). We compared the Arabidopsis and rice IQD gene families in terms of gene structure, chromosome location, predicted protein properties and motifs, phylogenetic relationships, and evolutionary history. The existence of an IQD-like gene in bryophytes suggests that IQD proteins are an ancient family of calmodulin-binding proteins and arose ...
We identified and analyzed 33 and 29 IQD1-like genes in Arabidopsis thaliana and Oryza sativa, respectively. The encoded IQD proteins contain a plant-specific domain of 67 conserved amino acid residues, referred to as the IQ67 domain, which is characterized by a unique and repetitive arrangement of three different calmodulin recruitment motifs, known as the IQ, 1-5-10, and 1-8-14 motifs. We demonstrated calmodulin binding for IQD20, the smallest IQD protein in Arabidopsis, which consists of a C-terminal IQ67 domain and a short N-terminal extension. A striking feature of IQD proteins is the high isoelectric point (~10.3) and frequency of serine residues (~11%). We compared the Arabidopsis and rice IQD gene families in terms of gene structure, chromosome location, predicted protein properties and motifs, phylogenetic relationships, and evolutionary history. The existence of an IQD-like gene in bryophytes suggests that IQD proteins are an ancient family of calmodulin-binding proteins and arose ...
adducin: membrane-skeleton associated calmodulin-binding protein of erythrocytes; major substrate for Ca+- & phospholipid-dependent protein kinase C; alpha-beta heterodimer with subunits of MW 103kDa (alpha) & 97kDa (beta); human erythrocyte GenBank X58199; alternatively spliced human transcript GenBank U43959
The A and C subunits of PP2A were previously shown to bind to striatin family complexes but the region of striatin to which the A/C heterodimer bound was unknown [8]. Our current data show that the coiled-coil domain of striatin mediates the formation of this PP2A heterotrimer (Figure 10). Although we cannot rule out a direct contribution of the caveolin-binding motif, the fact that double or triple point mutations in the middle of the coiled-coil/oligomerization domain of striatin almost completely disrupt PP2A association suggests that the primary determinants for PP2A binding are near the middle of the coiled-coil domain (residues 84-105).. Our data also suggest that PP2A association with striatin is dependent on oligomerization of striatin complexes. Of the charged coiled-coil residues that were mutated to disrupt PP2A binding to striatin, only arginine 88 is predicted to be at the dimerization interface when either NCOILS [33] or Paircoil2 [34] prediction programs are used, while the other ...
ConclusionThis study suggested that the C allele of XRCC1 had an 18% significantly decreased risk of TC in Chinese, and there were no significant associations among Caucasians under all genetic models....
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p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Caldesmon interaction with smooth muscle myosin and its ability to cross-link actin filaments to myosin were investigated by the use of several bacterially expressed myosin-binding fragments of caldesmon. We have confirmed the presence of two functionally different myosin-binding sites located in domains 1 and 3/4a of caldesmon. The binding of the C-terminal site is highly sensitive to ionic strength and hardly participates in acto-myosin cross-linking, while the N-terminal binding site is relatively independent of ionic strength and apparently contains two separate myosin contact regions within residues 1-28 and 29-128 of chicken gizzard caldesmon. Both these N-terminal sub-sites are involved in the interaction with myosin and are predominantly responsible for the caldesmon-mediated high-affinity cross-linking of actin and myosin filaments, without affecting the affinity of direct acto-myosin interaction. Binding of caldesmon and its fragments to myosin or rod filaments revealed affinity in the ...
Glucosinolates are a class of secondary metabolites with important roles in plant defense and human nutrition. To uncover regulatory mechanisms of glucosinolate production, we screened Arabidopsis thaliana T-DNA activation-tagged lines and identified a high-glucosinolate mutant caused by overexpress …
BACKGROUND: Activation and consequent phenotypic modulation of mesangial cells is considered to play a crucial role in the process of glomerular disease progression. Caldesmon, a calmodulin and actin-binding protein, is a molecular marker of the phenotypic change in smooth-muscle cells. SUBJECTS AND METHODS: We studied whether the expression of caldesmon in mesangial cells was enhanced in the process of IgA nephropathy and whether it would be a marker of mesangial activation indicating prognostic significance in specific disease states. We performed immunohistochemical staining with anticaldesmon and alpha-smooth-muscle actin (alpha-SMA) antibodies in 32 biopsy specimens from IgA nephropathy patients and analysed them quantitatively with a computer-aided manipulator. RESULTS: The glomerular expression of caldesmon was enhanced in IgA nephropathy patients. We compared caldesmon expression with composite histological scores (cell score and matrix score), clinical parameters and expressions of ...
The calcium-signaling network is an important transducer of internal and external stimuli in plants. These signals are transduced by a divers set of calcium-sensors such as calmodulin and calmodulin like proteins. In this work, AFG1L2 (AFG1 like protein 2) was characterized as a calmodulin binding protein that belongs to the family of AAA+ proteins (ATPases associated with various cellular activities). Using GFP fusion constructs it was possible to determine the in vivo localization of AFG1L2 in mitochondria and also to confirm the dual localization of a previously described homologe, AFG1L1, to mitochondria and chloroplasts. The interaction between AFG1L2 and calmodulin is calcium dependent and the calmodulin binding site of the AFG1L2 protein is in the AAA domain at a site homologous to the AFG1L1 protein, in very close proximity to the Walker A Motif, which is essential for ATP hydrolysis. It could be shown that ATP and ADP intensify the interaction between AFG1L2 and calmodulin. AAA-proteins ...
Transcription factor regulating the cell cycle specific transcription of a spindle pole body (SPB) calmodulin binding protein SPC110. Required for full induction of SPC110 transcription in late G1. Binds to DNA consensus sequence 5-[AT]AA[TC]AAACAA[AT]-3. Dosage dependent suppressor of calmodulin mutants which have specific defects in SPB assembly.
The binding of chicken gizzard caldesmon to actin was studied both in the presence and the absence of caltropin using Airfuge centrifugation experiments, disulfide cross-linking studies, and the fluorescent probe acrylodan (6-acryloyl-2-(dimethylamino)naphthalene). In co-sedimentation studies most of the caldesmon pelleted along with actin. However, when caldesmon in the presence of caltropin was mixed with actin, caldesmon did not pellet along with actin following high speed centrifugation, suggesting that caltropin has significantly weakened its binding to actin. The caltropin effect was noticed even when tropomyosin was included in the reaction mixture. Acrylodan-labeled caldesmon, when excited at 375 nm, had an emission maximum at 515 +/- 2 nm. The addition of actin produced a nearly 70% increase in fluorescent intensity, accompanied by a blue shift in the emission maximum (i.e. lambda em (max) = 505 +/- 2 nm), suggesting that the probe now occupies a more nonpolar environment. Titration of labeled
Alpha-adducin is a protein that in humans is encoded by the ADD1 gene. Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. Polymorphism in ADD1 is associated with hypertension. GRCh38: Ensembl release 89: ENSG00000087274 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000029106 - Ensembl, May 2017 Human PubMed ...
Adducin is a protein recently purified from erythrocytes and brain that has properties in in vitro assays suggesting a role in assembly of a spectrin-actin lattice. This report describes the localization of adducin to plasma membranes of a variety of tissues and the discovery that adducin is concentrated at sites of cell-cell contact in the epithelial tissues where it is expressed. Adducin in tissues and cultured cells always was observed in association with spectrin and actin, although spectrin and actin were evident in the absence of adducin. In sections of intestinal epithelial cells spectrin was present on all plasma membrane surfaces while adducin was restricted to the lateral cell borders. Adducin also was not detected in association with actin stress fibers in cultured cells. The presence of adducin at cell-cell contact sites of cultured epithelial cells requires extracellular Ca++ and occurs within 15 min of addition of 0.3 mM Ca++. Redistribution of adducin after addition of ...
The key finding of our study was that in a randomly recruited sample of middle-aged and older white subjects, ACE DD homozygosity predicted total and cardiovascular mortality and the incidence of cardiovascular events in carriers of the mutated ADD1 but not in subjects carrying the wild-type ADD1. These findings translate previously observed11-15 associations of cardiovascular risk factors with these 2 polymorphisms into hard cardiovascular outcomes. Furthermore, in ex vivo studies, human fibroblasts showed higher membrane-bound ACE activity in the presence of the ADD1 Trp allele. We corroborated the latter observation in cell membranes of HEK cells transfected with the human mutated and wild-type ADD1. Based on this replication, we believe that our ex vivo finding might be extrapolated to human kidney cells.. In line with expert opinion,24 we opted for the candidate gene approach, which relies on solid knowledge of pathophysiologic pathways and accounts for the context dependency of genetic ...
目的:探讨浅表性平滑肌肉瘤(superficial leiomyosarcoma,SLMS)的临床病理特点、诊断、鉴别诊断及治疗和预后。方法:回顾性分析13例SLMS的临床病理特征,并复习相关文献讨论。结果:男7例,女6例,平均年龄59岁。肿瘤位于四肢7例,躯干3例,头面部2例,阴囊1例,其中位于真皮的9例,皮下4例。肿瘤直径1~8(平均4.3)cm。局部复发5例,无远处转移病例。镜下:肿瘤呈结节状或弥漫状生长,根据肿瘤细胞不同分化分为三型:中分化(最多见,9例)、高分化和低分化型(较少,各2例)。免疫组织化学:肿瘤细胞desmin,MSA,a-SMA和h-caldesmon阳性。结论:SLMS多发生于中老年人,预后较好,易发生局部复发,极少发生远处转移。治疗采用局部扩大切除。
Ca2+-calmodulin binding to caldesmon and the caldesmon-actin-tropomyosin complex. Its role in Ca2+regulation of the activity of synthetic smooth-muscle thin filaments Academic Article ...
Related Articles The predictive performance of short-linear motif features in the prediction of calmodulin-binding proteins. BMC Bioinformatics. 2018 Nov 20;19(Suppl 14):410 Authors: Li Y, Maleki ...
Heterogeneity of SMC is a well-known phenomenon. The interpretation, however, of the morphologic phenotype as well as the functional implications of the expression of differentiation markers is subject to much debate. The data are derived from in vivo or in vitro studies. The material used varies from adult to neonatal to fetal vessels and cells from different animal species. The origin of the cultured cells can be from intima, inner or outer media, or the adventitia in both normal and diseased vessels.. The data from in vitro studies are largely reflected in in vivo studies. Proceeding from data in early fetal development, it has been shown that the SMC start to express SM α-actin17 followed by 1E12,20 an actinin marker, and smoothelin.21 Relative late differentiation markers are the already mentioned markers, such as SM22, calponin, h-caldesmon,7 and SM myosin.8 These are upregulated until the expression level of the mature vessel wall is reached.9 11 During this developmental period, there ...
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Calcineurin B兔多克隆抗体(ab94535)可与人样本反应并经WB实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Plasmid Actin-53b-ires-EGFP from Dr. Jerry Crabtrees lab contains the insert BAF53b and is published in Neuron. 2007 Oct 4. 56(1):94-108. This plasmid is available through Addgene.
What function might a flagellar membrane-associated CrKCBP have? One function might include a role in intraflagellar transport (IFT). IFT involves a plus-end-directed heterotrimeric kinesin as well as a minus-end-directed cytoplasmic dynein and is required for the assembly, disassembly and structural maintenance of cilia and flagella (for a review, see Cole, 2003). Presumably, a minus-end-directed kinesin (CrKCBP) and cytoplasmic dynein would have redundant functions in IFT. However, it is possible that two minus-end-directed motors are required during rapid flagellar resorption. In Chlamydomonas, flagellar resorption can be induced by removal of Ca2+ from the medium and is reversed by re-addition of Ca2+ to the medium (Lefebvre et al., 1978). If CrKCBP is negatively regulated by Ca2+-calmodulin in the same way as AtKCBP (see Song et al., 1997), removal of Ca2+ from the medium would activate KCBP, consistent with a role for CrKCBP during flagellar resorption.. Several key observations provide ...
TY - CHAP. T1 - Biochemistry and Pharmacology of Calmodulin-Regulated Phosphatase Calcineurin. AU - Perrino, Brian A.. AU - Soderling, Thomas R.. PY - 2012/12/2. Y1 - 2012/12/2. UR - http://www.scopus.com/inward/record.url?scp=84941124499&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=84941124499&partnerID=8YFLogxK. U2 - 10.1016/B978-0-08-092636-0.50008-6. DO - 10.1016/B978-0-08-092636-0.50008-6. M3 - Chapter. AN - SCOPUS:84941124499. SN - 9780127138602. SP - 169. EP - 236. BT - Calmodulin and Signal Transduction. PB - Elsevier Inc.. ER - ...
Sigma-Aldrich offers abstracts and full-text articles by [Jampani Nageswara Rao, Roland Rivera-Santiago, Xiaochuan Edward Li, William Lehman, Roberto Dominguez].
connectin: cell surface protein which binds both laminin & actin; binds thick filaments to Z lines in frog skeletal muscle; see also record for mini-titin which is a similar protein from invertebrates; titin has a protein kinase domain and a calmodulin-binding site near the C-terminus
A membrane skeleton protein (heterodimer) that interacts with a junctional complex that links spectrin assemblies. The complex in the red blood cell consists of tropomyosin and actin with band 4.1 (see band). Adducin probably binds to band 4.1.. [...] ...
Complete information for CALD1 gene (Protein Coding), Caldesmon 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Calcineurin A; One Isoform (the Other Is Cmp2p) Of The Catalytic Subunit Of Calcineurin, A Ca++/calmodulin-regulated Protein Phosphatase Which Regulates Crz1p (a Stress-response Transcription Factor), The Other Calcineurin Subunit Is CNB1; Regulates The Function Of Aly1p Alpha-arrestin; CNA1 Has A Paralog, CMP2, That Arose From The Whole Genome Duplication
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Calponin兔多克隆抗体(ab111183)可与人样本反应并经WB, IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Microtubules (MTs) and actin filaments (F-actin) function cooperatively to regulate plant cell morphogenesis. However, the mechanisms underlying the crosstalk between these two cytoskeletal systems, particularly in cell shape control, remain largely unknown. Here, we show that introduction of the MyTH4-FERM tandem into KCBP (Kinesin-like Calmodulin-Binding Protein) during evolution conferred novel functions. The MyTH4 domain and the FERM domain in the N-terminal tail of KCBP physically bind to MTs and F-actin, respectively. During trichome morphogenesis, KCBP distributes in a specific cortical gradient, and concentrates at the branching sites and the apexes of elongating branches, which lack MTs but have cortical F-actin. Further live-cell imaging and genetic analyses revealed that KCBP acts as a hub integrating MTs and actin filaments to assemble the required cytoskeletal configuration for the unique, polarized diffuse growth pattern during trichome cell morphogenesis. Our findings provide ...
The calponin family of F-actin-, tropomyosin- and calmodulin-binding proteins currently comprises three genetic variants. Their functional roles implicated from in vitro studies include the regulation of actomyosin interactions in smooth muscle cells (h1 calponin), cytoskeletal organisation in non-muscle cells (h2 calponin) and the control of neurite outgrowth (acidic calponin). We have now investigated the effects of calponin (CaP) isoforms and their C-terminal deletion mutants on the actin cytoskeleton by time lapse video microscopy of GFP fusion proteins in living smooth muscle cells and fibroblasts. It is shown that h1 CaP associates with the actin stress fibers in the more central part of the cell, whereas h2 CaP localizes to the ends of stress fibres and in the motile lamellipodial protrusions of spreading cells. Cells expressing h2 CaP spread more efficiently than those expressing h1 CaP and expression of GFP h1 CaP resulted in reduced cell motility in wound healing experiments. Notably, ...
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Adducin is a membrane-skeletal protein which is a candidate to promote assembly of a spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. The complete sequence of both subunits of human adducin, alpha (737 amino acids), and beta (726 amino acids) has been deduced by analysis of the cDNAs. The two subunits have strikingly conserved amino acid sequences with 49% identity and 66% similarity, suggesting evolution by gene duplication. Each adducin subunit has three distinct domains: a 39-kD NH2-terminal globular protease-resistant domain, connected by a 9-kD domain to a 33-kD COOH-terminal protease-sensitive tail comprised almost entirely of hydrophilic amino acids. The tail is responsible for the high frictional ratio of adducin noted previously, and was visualized by EM. The head domains of both adducin subunits exhibit a limited sequence similarity with the NH2-terminal actin-binding motif present in members of the spectrin superfamily and actin gelation ...
Calcineurin (CN), a calcium- and calmodulin-dependent protein phosphatase, plays a significant role in the central nervous system. Previously, we reported that forebrain-specific CN knockout mice (CN mutant mice) have impaired working memory. To further analyze the behavioral effects of CN deficiency, we subjected CN mutant mice to a comprehensive behavioral test battery. Mutant mice showed increased locomotor activity, decreased social interaction, and impairments in prepulse inhibition and latent inhibition. In addition, CN mutant mice displayed an increased response to the locomotor stimulating effects of MK-801. Collectively, the abnormalities of CN mutant mice are strikingly similar to those described for schizophrenia. We propose that alterations affecting CN signaling could comprise a contributing factor in schizophrenia pathogenesis.. ...
This report describes Ca2+-dependent binding of 125I-labeled calmodulin (125I-CaM) to erythrocyte membranes and identification of two new CaM-binding proteins. Erythrocyte CaM labeled with 125I-Bolton Hunter reagent fully ...
Two neuron-specific proteins, neurogranin (Ng), and neuromodulin (GAP43), were studied. Our first aim was to know the potentiality of various chemicals for their induction of Ng mRNA expression; our second one was to know the intracellular localization of GAP43 and its co-localization with calmodulin (CaM). Our results indicated that although some of the NO donors could enhance Ng promoter activity, they could not induce Ng mRNA expression in HEK293 and PC12 cells. Moreover, our findings revealed that Ng mRNA could be induced only by NGF and only in PC12 cells. For GAP43b s intracellular localization and co-localization with CaM, our results revealed DsRed-GAP43 and its mutants (S41D, S41G) were expressed mainly in the region near to cell membrane while EGFP-GAP43 and its mutants (S41D, S41G) were mostly distributed in cytoplasm. PMA treatment did not affect the localization of GAP43. For the co-transfection experiments, a particular region of co-localization of GAP43 and CaM was found to be ...
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The time course of interaction of caldesmon with actin may be monitored by fluorescence changes that occur upon the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or to acrylodan ...
NRGN - NRGN (untagged)-Human neurogranin (protein kinase C substrate, RC3) (NRGN), transcript variant 1 available for purchase from OriGene - Your Gene Company.
View mouse Camta1 Chr4:151059525-151861876 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
EWSR1 - EWSR1 (Myc-DDK-tagged)-Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 1 available for purchase from OriGene - Your Gene Company.
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... is a protein that in humans is encoded by the CALD1 gene. Caldesmon is a calmodulin binding protein. Like calponin, ... This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and ... Sobue K, Muramoto Y, Fujita M, Kakiuchi S (Sep 1981). "Purification of a calmodulin-binding protein from chicken gizzard that ... The conserved domain of this protein possesses the binding activities to Ca++-calmodulin, actin, tropomyosin, myosin, and ...
... nuclear autoantigen are members of a novel family of calmodulin-binding proteins that associate with protein phosphatase 2A". ... "Entrez Gene: STRN striatin, calmodulin binding protein". Goudreault M, D'Ambrosio LM, Kean MJ, Mullin MJ, Larsen BG, Sanchez A ... Gaillard S, Bartoli M, Castets F, Monneron A (Nov 2001). "Striatin, a calmodulin-dependent scaffolding protein, directly binds ... "A novel calmodulin-binding protein, belonging to the WD-repeat family, is localized in dendrites of a subset of CNS neurons". ...
Calcium binds to the protein calmodulin (CaM). When bound to calcium, CaM binds to the CaM-binding domain on the intracellular ... When each of the four CaM-binding domain subunits is bound to calmodulin, the SK channel changes conformation. This transitions ... bound to the cytoplasmic C-terminus region of the peptide called the calmodulin binding domain (CaMBD). Additional association ... In addition, SK potassium channels are tightly associated with the protein calmodulin, which accounts for the calcium ...
... nuclear autoantigen are members of a novel family of calmodulin-binding proteins that associate with protein phosphatase 2A". J ... "Entrez Gene: STRN3 striatin, calmodulin binding protein 3". Goudreault M, D'Ambrosio LM, Kean MJ, Mullin MJ, Larsen BG, Sanchez ... and striatin are calmodulin-binding, WD repeat proteins principally expressed in the brain". J. Biol. Chem. 275 (26): 19970-7. ... "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3: 89. doi:10.1038/msb4100134 ...
"Entrez Gene: STRN4 striatin, calmodulin binding protein 4". Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom- ... Gaillard S, Bartoli M, Castets F, Monneron A (2001). "Striatin, a calmodulin-dependent scaffolding protein, directly binds ... and striatin are calmodulin-binding, WD repeat proteins principally expressed in the brain". J. Biol. Chem. 275 (26): 19970-7. ... "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/ ...
Valencia CA, Ju W, Liu R (2007). "Matrin 3 is a Ca2+/calmodulin-binding protein cleaved by caspases". Biochem. Biophys. Res. ... 1998). "Mapping of human DNA-binding nuclear protein (NP220) to chromosome band 2p13.1-p13.2 and its relation to matrin 3". ... Matrin-3 is a protein that in humans is encoded by the MATR3 gene. The protein encoded by this gene is localized in the nuclear ... 2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. ...
"Enkurin is a novel calmodulin and TRPC channel binding protein in sperm". Dev. Biol. 274 (2): 426-35. doi:10.1016/j.ydbio. ... and functions as an adaptor protein, tethering signal transduction proteins to TRPC channels. GRCh38: Ensembl release 89: ... Enkurin is a protein that in humans is encoded by the ENKUR gene. Enkurin interacts with transient receptor potential canonical ...
Calmodulin-like protein 5 is a protein that in humans is encoded by the CALML5 gene. This gene encodes a novel calcium binding ... 2006). "Influence of calcium on the proteolytic degradation of the calmodulin-like skin protein (calmodulin-like protein 5) in ... protein expressed in the epidermis and related to the calmodulin family of calcium binding proteins. Functional studies with ... 2002). "Cation- and peptide-binding properties of human calmodulin-like skin protein". Biochemistry. 41 (17): 5439-48. doi: ...
Karp CM, Shukla MN, Buckley DJ, Buckley AR (2007). "HRPAP20: a novel calmodulin-binding protein that increases breast cancer ... NADH:ubiquinone oxidoreductase complex assembly factor 4 is a protein that in humans is encoded by the NDUFAF4 gene. NADH: ... interacting complex I assembly protein, cause fatal neonatal mitochondrial disease". Am. J. Hum. Genet. 84 (6): 718-27. doi: ...
Flory MR, Moser MJ, Monnat RJ, Davis TN (May 2000). "Identification of a human centrosomal calmodulin-binding protein that ... Flory MR, Moser MJ, Monnat RJ, Davis TN (May 2000). "Identification of a human centrosomal calmodulin-binding protein that ... PCNT has been shown to interact with: calmodulin, separase, CEP215, CHD3/4, protein kinase A, protein kinase C, DISC1, γ- ... This protein localizes to the centrosome and recruits proteins to the pericentriolar matrix (PCM) to ensure proper centrosome ...
phosphatidylinositol phosphate binding. • phosphatidylserine binding. • calmodulin binding. • protein binding. Cellular ... Growth Associated Protein 43 also known as GAP43 is a protein that in humans is encoded by the GAP43 gene.[5] ... neuron growth-associated protein 43. Function[edit]. GAP43, is a nervous tissue-specific cytoplasmic protein that can be ... protein kinase C-activating G-protein coupled receptor signaling pathway. • response to wounding. • regulation of growth. • ...
"The interaction between PSD-95 and Ca2+/calmodulin is enhanced by PDZ-binding proteins". J Biochem. 138 (2): 177-82. doi: ... Cysteine-rich PDZ-binding protein is a protein that in humans is encoded by the CRIPT gene. CRIPT has been shown to interact ... Cai C, Coleman SK, Niemi K, Keinänen K (2002). "Selective binding of synapse-associated protein 97 to GluR-A alpha-amino-5- ... "Entrez Gene: CRIPT cysteine-rich PDZ-binding protein". Lim, Indra Adi; Hall Duane D; Hell Johannes W (Jun 2002). "Selectivity ...
This gene encodes a protein containing multiple ankyrin domains and two IQ calmodulin-binding domains. The encoded protein may ... A similar protein in mice interacts with calmodulin. Mutations in this gene have been associated with nephronophthisis type 2. ... 2005). "Nephrocystin-5, a ciliary IQ domain protein, is mutated in Senior-Loken syndrome and interacts with RPGR and calmodulin ... 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038 ...
"Differential binding of calmodulin-related proteins to their targets revealed through high-density Arabidopsis protein ... For example, calcium ions bind to the EF hand domains of calmodulin, allowing it to bind and activate calmodulin-dependent ... Many adaptor proteins and enzymes activated as part of signal transduction possess specialized protein domains that bind to ... Other activated proteins interact with adaptor proteins that facilitate signaling protein interactions and coordination of ...
Deloulme JC, Prichard L, Delattre O, Storm DR (1997). "The prooncoprotein EWS binds calmodulin and is phosphorylated by protein ... RNA-binding protein EWS is a protein that in humans is encoded by the EWSR1 gene on human chromosome 22. This gene encodes a ... "Cloning and mapping of a human RBP56 gene encoding a putative RNA binding protein similar to FUS/TLS and EWS proteins". ... "Tyrosine kinase Pyk2 mediates G-protein-coupled receptor regulation of the Ewing sarcoma RNA-binding protein EWS". Curr. Biol. ...
"The prooncoprotein EWS binds calmodulin and is phosphorylated by protein kinase C through an IQ domain". The Journal of ... Krev interaction trapped protein 1 is a protein that in humans is encoded by the CCM1 gene. This gene contains 16 coding exons ... "KRIT1 association with the integrin-binding protein ICAP-1: a new direction in the elucidation of cerebral cavernous ... "KRIT1 association with the integrin-binding protein ICAP-1: a new direction in the elucidation of cerebral cavernous ...
Many of Ca2+-mediated events occur when the released Ca2+ binds to and activates the regulatory protein calmodulin. Calmodulin ... or may act directly on other effector proteins. Besides calmodulin, there are many other Ca2+-binding proteins that mediate the ... Certain proteins of the cytoplasm and organelles act as buffers by binding Ca2+. Signaling occurs when the cell is stimulated ... DAG recruits protein kinase C (PKC), attaching it to the plasma membrane IP3 diffuses to the endoplasmic reticulum, and binds ...
... a hyaluronan-binding protein that regulates ras signaling, correlates with overexpression of mitogen-activated protein kinase ... Lynn BD, Turley EA, Nagy JI (Jul 2001). "Subcellular distribution, calmodulin interaction, and mitochondrial association of the ... RHAMM also binds directly with ERK1 and forms complexes with ERK1,2 and MEK1, suggesting a role as a scaffold protein that ... Turley EA, Austen L, Vandeligt K, Clary C (Mar 1991). "Hyaluronan and a cell-associated hyaluronan binding protein regulate the ...
Bauer MC, O'Connell D, Cahill DJ, Linse S (June 2008). "Calmodulin binding to the polybasic C-termini of STIM proteins involved ... No additional STIM-like proteins have been identified until now in vertebrates. STIM2 protein is a type I transmembrane protein ... EF-hand Ca2+-binding motif discovered recently and a sterile a-motif (SAM) domain, a well-known protein-protein interaction ... The coiled-coil domains mediate interactions between STIM proteins, allowing them to bind each other and form homo and ...
This is likely related to the calcium-binding protein calmodulin, suggesting Ca2+ involvement in mediating growth responses. ... Braam J, Davis RW (1990). "Rain-, wind-, and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis ... Erner, Y; Jaffe, MJ (1983). "Thigmomorphogenesis: Membrane lipid and protein changes in bean plants as affected by mechanical ...
"A relationship between protein kinase C phosphorylation and calmodulin binding to the metabotropic glutamate receptor subtype 7 ... Enz R (2002). "The actin-binding protein Filamin-A interacts with the metabotropic glutamate receptor type 7". FEBS Lett. 514 ( ... and syntenin bind multiple glutamate receptor subtypes. Analysis of PDZ binding motifs". J. Biol. Chem. 277 (18): 15221-4. doi: ... Metabotropic glutamate receptor 7 is a protein that in humans is encoded by the GRM7 gene. L-glutamate is the major excitatory ...
"Structure and expression of a calcium-binding protein gene contained within a calmodulin-regulated protein kinase gene". ... Vorotnikov AV (1997). "Kinase-related protein: a smooth muscle myosin-binding protein". Int. J. Biochem. Cell Biol. 29 (5): 727 ... Kinase-related protein (telokin) binds to dephosphorylated smooth myosin near the junction between the rod and the catalytic ... The binding of KRP to the neck region folded, ATP-bound dephosphorylated myosin also promotes unfolding and filament formation ...
The isoform contains three copies and serves as a binding site for Calmodulin or CaM-like proteins. The Leucine-Rich Repeat ... The protein is a nuclear encoding mitochondrial protein. The protein in humans has 1760 amino acids. The protein is considered ... The protein binds DNA on N-box motifs. To date, the clinical significance of this gene is not known. GRCh38: Ensembl release 89 ... The protein is likely a nuclear encoding mitochondrial protein and is found in all Metazoans. LRRIQ1 is mapped at on chromosome ...
"Identification and characterization of a calmodulin-binding domain in Ral-A, a Ras-related GTP-binding protein purified from ... "Identification and characterization of a novel protein interacting with Ral-binding protein 1, a putative effector protein of ... "Identification and characterization of a novel protein interacting with Ral-binding protein 1, a putative effector protein of ... Wang KL, Roufogalis BD (May 1999). "Ca2+/calmodulin stimulates GTP binding to the ras-related protein ral-A". The Journal of ...
This gene encodes a protein with high similarity to the calcium-binding proteins of the calmodulin family. The encoded protein ... Calcium-binding protein 8 is a protein that in humans is encoded by the CALN1 gene. Alternative splicing results in multiple ... "Entrez Gene: CALN1 calneuron 1". McCue HV, Haynes LP, Burgoyne RD (Aug 2010). "The diversity of calcium sensor proteins in the ... a new member of the calmodulin superfamily". Molecular Genetics and Metabolism. 72 (4): 343-50. doi:10.1006/mgme.2001.3160. ...
Phillips AM, Bull A, Kelly LE (April 1992). "Identification of a Drosophila gene encoding a calmodulin-binding protein with ... Check date values in: ,access-date= (help) "TRIP Database". a manually curated database of protein-protein interactions for ... The identity of the mutated protein was unknown until it was cloned by Craig Montell, a post-doctoral researcher in Gerald ... TRPs have important interactions with other proteins and often form signaling complexes, the exact pathways of which are ...
This gene encodes a microtubule-associated protein (MAP). The encoded protein is a calmodulin-binding and calmodulin-regulated ... Microtubule-associated protein 6 (MAP6) or stable tubule-only polypeptide (STOP or STOP protein) is a protein that in humans is ... The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 8 (2): 85-95. doi: ... "Entrez Gene: MAP6 microtubule-associated protein 6". Bosc C, Cronk JD, Pirollet F, et al. (1996). "Cloning, expression, and ...
This domain binds calmodulin, a protein known as a calcium sensor that can bind and regulate many target proteins. A GRD ( ... which mediates actin-binding and binds calponin. The WW, or poly-proline protein-protein domain, so named because of two ... Hart MJ, Callow MG, Souza B, Polakis P (Aug 1996). "IQGAP1, a calmodulin-binding protein with a rasGAP-related domain, is a ... Bashour AM, Fullerton AT, Hart MJ, Bloom GS (1997). "IQGAP1, a Rac- and Cdc42-binding protein, directly binds and cross-links ...
Calmodulin is a symmetrical, hinge-like protein that binds to four calcium ions via E-F motifs. When calcium is present, CaM ... Ca2+ binding to the calmodulin moiety results in a structural shift that eliminates this solvent pathway, rapid de-protonation ... GCaMP is created from a fusion of green fluorescent protein (GFP), calmodulin, and M13, a peptide sequence from myosin light ... and the hinge region is able to bind helical peptide chains on target proteins, such as M13. In the absence of calcium, the ...
Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing ... Definition of the calmodulin-binding domain and sites of phosphorylation by protein kinases A and C". J. Biol. Chem. 271 (41): ... Alpha-adducin is a protein that in humans is encoded by the ADD1 gene. Adducins are a family of cytoskeleton proteins encoded ... Calcium/calmodulin-regulated capping of the barbed ends of actin filaments". J. Biol. Chem. 271 (14): 7986-91. doi:10.1074/jbc. ...
metal ion binding. • voltage-gated ion channel activity. • ion channel activity. • protein binding. • alpha-actinin binding. • ... When depolarization occurs, calcium ions flow through the channel and some bind to Calmodulin. The Calcium/Calmodulin binding ... When calcium ions bind to calmodulin, which in turn binds to a Cav1.2 channel, it allows the Cav1.2 channels within a cluster ... calmodulin binding. Cellular component. • cytoplasm. • voltage-gated calcium channel complex. • integral component of membrane ...
lipid binding. Cellular component. • cytoplasm. • cyclin-dependent protein kinase 5 holoenzyme complex. • plasma membrane. • ... "The cyclin-dependent kinase 5 activators p35 and p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein ... positive regulation of protein kinase activity. • regulation of cyclin-dependent protein serine/threonine kinase activity. • ... Agarwal-Mawal A, Paudel HK (2001). "Neuronal Cdc2-like protein kinase (Cdk5/p25) is associated with protein phosphatase 1 and ...
cAMP binds to and releases an active form of protein kinase A (PKA). Next, PKA phosphorylates phosphorylase kinase, which, in ... The increased calcium availability binds to the calmodulin subunit and activates glycogen phosphorylase kinase. Glycogen ... Residues 397-437 form this structure, which allows the protein to covalently bind to the glycogen chain a full 30 Å from the ... Binding of AMP at this site, corresponding in a change from the T state of the enzyme to the R state, results in small changes ...
receptor binding. • neurotrophin TRKB receptor binding. • growth factor activity. • GO:0001948 protein binding. ... Zhong L, Yan CH, Lu CQ, Xu J, Huang H, Shen XM (September 2009). "Calmodulin activation is required for the enhancement of ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... positive regulation of receptor binding. • regulation of protein localization to cell surface. • regulation of receptor ...
cyclin-dependent protein serine/threonine kinase regulator activity. • protein binding. • ATP binding. • cyclin binding. • ... Taulés M, Rius E, Talaya D, López-Girona A, Bachs O, Agell N (1998). "Calmodulin is essential for cyclin-dependent kinase 4 ( ... nucleotide binding. • protein kinase activity. • kinase activity. • protein serine/threonine kinase activity. • ... The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene ...
One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag. In: Protein ... Calmodulin-Tag (CaM-Tag, Sequenz: KRRWKKNFIAVSAANRFKKISSSGAL). *Chitin-bindendes-Protein-Tag (CBP-Tag) ... ein C-terminales Protein-Tag am Protein während der Translation. Gelegentlich muss das Protein-Tag vom Protein nach der ... Zur Erzeugung eines Protein-Tags wird die codierende DNA-Sequenz des Protein-Tags unter Erhalt des Leserasters in die ...
... regulate G proteins in several different ways. Small GTPases act as molecular switches in ... Another class of regulatory proteins, the Guanosine nucleotide dissociation inhibitors (GDIs), bind to the GDP-bound form of ... between the GTP-bound and GDP-bound form, regulated by other regulatory proteins. ... They are active or 'ON' when it is bound to GTP and inactive or 'OFF' when bound to GDP.[1] Activation and deactivation of ...
The activity of A2A adenosine receptor, a G-protein coupled receptor family member, is mediated by G proteins that activate ... Also recently discovered A2B has Gq → DAG and IP3 → Release calcium → activate calmodulin → activate myosin light chain kinase ... overview on a druggable GPCR and on structure-activity relationship analysis and binding requirements of agonists and ... This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts ...
... protein kinase A (PKA), protein kinase C, Cdc2, and/or a Ca2+/calmodulin-dependent protein kinase; b) moves to bind with ... ALOX5 binds with the F actin-binding protein, coactin-like protein. Based on in vitro studies, this protein binding serves to ... iron ion binding. • arachidonate 5-lipoxygenase activity. • dioxygenase activity. • metal ion binding. • protein binding. • ... A proline-rich region (residues 566-577), sometimes termed a SH3-binding domain, which promotes its binding to proteins with ...
a b c d e f g h i j k l m Petsko, GA and Ringe, D 2009, 'Protein Structure and Function', Oxford University Press Inc., New ... Another example is the binding of insulin to insulin receptors. Once released into the bloodstream insulin can bind to ... "Structure of the CaMKIIdelta/calmodulin complex reveals the molecular mechanism of CaMKII kinase activation". PLoS Biology. 8 ... "Structure-based drug design enables conversion of a DFG-in binding CSF-1R kinase inhibitor to a DFG-out binding mode". ...
Each tropomyosin molecule has a smaller calcium-binding protein called troponin bound to it. All thin filaments are attached to ... The protein complex composed of actin myosin, contractile proteins, is sometimes referred to as "actomyosin". In striated ... Troponin and the associated tropomyosin undergo a conformational change after calcium binding and expose the myosin binding ... Myofilaments are the filaments of myofibrils, constructed from proteins,[1] principally myosin or actin. Types of muscle are ...
... between the microtubule-associated tau proteins and S100b regulate tau phosphorylation by the Ca2+/calmodulin-dependent protein ... "alpha-synuclein binds to Tau and stimulates the protein kinase A-catalyzed tau phosphorylation of serine residues 262 and 356" ... Protein tau (bahasa Inggris: microtubule-associated protein tau, MAPT) adalah protein yang membuat mikrotubula menjadi stabil. ... Proses fosforilasi juga dikatalisasi oleh cAMP-dependent protein kinase setelah protein tau terikat pada protein 14-3-3zeta.[3] ...
... while GluA2 binds to PICK1[9] and GRIP/ABP. Of note, AMPARs cannot directly bind to the common synaptic protein PSD-95 owing to ... "Calcium/calmodulin-dependent protein kinase II phosphorylation drives synapse-associated protein 97 into spines". J. Biol. Chem ... All AMPARs contain PDZ-binding domains, but which PDZ domain they bind to differs. For example, GluA1 binds to SAP97 through ... "Synaptic anchorage of AMPA receptors by cadherins through neural plakophilin-related arm protein AMPA receptor-binding protein ...
ATP binding. • calmodulin binding. • protein binding. • minus-end directed microfilament motor activity. • ADP binding. • actin ... nucleotide binding. • cadherin binding. • identical protein binding. Cellular component. • myosin complex. • cell nucleus. • ... "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi: ... protein complex. • apical part of cell. • clathrin-coated vesicle membrane. • ruffle. • cytosol. • extracellular exosome. • ...
Calcium-binding proteins. Intracellular calcium-sensing proteins. *Calmodulin. *Calnexin. *Calreticulin. *Gelsolin. *neuronal * ... Calsequestrin is a calcium-binding protein of the sarcoplasmic reticulum. The protein helps hold calcium in the cisterna of the ... The active protein is not highly structured, more than 50% of it adopting a random coil conformation.[2] When calcium binds ... Each molecule of calsequestrin can bind 18 to 50 Ca2+ ions.[1] Sequence analysis has suggested that calcium is not bound in ...
protein binding. • ankyrin binding. • gamma-catenin binding. • beta-catenin binding. • GTPase activating protein binding. • ... Li Z, Kim SH, Higgins JM, Brenner MB, Sacks DB (December 1999). "IQGAP1 and calmodulin modulate E-cadherin function". The ... metal ion binding. • cell adhesion molecule binding. • cadherin binding. • identical protein binding. ... Oneyama C, Nakano H, Sharma SV (March 2002). "UCS15A, a novel small molecule, SH3 domain-mediated protein-protein interaction ...
... which is linked in the middle of the protein to a calmodulin-binding domain. Binding of calmodulin appears to act as a " ... In contrast, calmodulin remains tightly bound to the inducible and Ca2+-insensitive isoform (iNOS or NOS2) even at a low ... In NOS1 and NOS3, physiological concentrations of Ca2+ in cells regulate the binding of calmodulin to the "latch domains", ... The FMN binding domain is homologous to flavodoxins, and the two domain fragment containing the FAD and NADPH binding sites is ...
protein complex binding. • signal transducer activity. • protein binding. • GTPase activity. • GTPase binding. • G-protein ... Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 is a protein that in humans is encoded by the GNB1 gene.[5] ... Huang CL, Jan YN, Jan LY (1997). "Binding of the G protein betagamma subunit to multiple regions of G protein-gated inward- ... Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector ...
The protein ELKS binds to the cell adhesion protein, β-neurexin, and other proteins within the complex such as Piccolo and ... The increase in presynaptic calcium concentration activates calcium-calmodulin-dependent protein kinase (CaMK). CaMK ... It is stabilized by proteins within the active zone and bound to the presynaptic membrane by SNARE proteins. These vesicles are ... Neuroligin then interacts with proteins that bind to postsynaptic receptors. Protein interactions like that seen between ...
dopamine binding. • enzyme binding. • oxidoreductase activity. • iron ion binding. • ferric iron binding. • protein binding. • ... is phosphorylated by the calcium-calmodulin-dependent protein kinase.[27] MAPKAPK2 (mitogen-activated-protein kinase-activating ... amino acid binding. • monooxygenase activity. • protein domain specific binding. Cellular component. • cytoplasm. • cytosol. • ... ferrous iron binding. • oxygen binding. • metal ion binding. • ...
... a calmodulin-binding protein with a rasGAP-related domain, is a potential effector for cdc42Hs". The EMBO Journal. 15 (12): ... protein binding. • thioesterase binding. • protein kinase binding. • nucleotide binding. • GTP binding. • identical protein ... ubiquitin protein ligase activity. • apolipoprotein A-I receptor binding. • GTP-dependent protein binding. • GTPase activity. • ... "Protein Data Bank in Europe. EMBL-EBI. Retrieved 2016-04-22.. *^ "CDC42 (cell division cycle 42 (GTP binding protein, 25kDa))" ...
G proteins, also known as guanine nucleotide-binding proteins, are a family of proteins that act as molecular switches inside ... Types of G protein signaling[edit]. G protein can refer to two distinct families of proteins. Heterotrimeric G proteins, ... "Regulator of G protein signalling"). Receptors stimulate GTP binding (turning the G protein on). RGS proteins stimulate GTP ... Calcium-binding protein. *Calmodulin-binding proteins. *copper *Ceruloplasmin. *iron *Iron-binding proteins ...
calmodulin binding. • ATPase activity. • protein domain specific binding. • actin binding. • motor activity. • ATP binding. • ... nucleotide binding. • microfilament motor activity. • protein binding. • ADP binding. • protein membrane anchor. • actin- ... RNA binding. • cadherin binding. • actin filament binding. • microtubule motor activity. • microtubule binding. ... Other proteins that are known to interact with NM IIA include the actin binding protein tropomyosin 4.2 [26] and a novel actin ...
... due to compensating interactions with the protein. In the channels, the protein does not shield chloride ions at one binding ... It was regulated by Ca2+ via a calmodulin kinase II-dependent mechanism. Distant homologues may be present in plants, ciliates ... Each channel has two transmembrane domains and two nucleotide binding domains. ATP binding to both nucleotide binding domains ... Particular CLC transporters and proteins have modulated activity when bound with ATP, ADP, AMP, or adenosine at the CBS domains ...
protein kinase activity. • PDZ domain binding. • SH3 domain binding. • scaffold protein binding. • metal ion binding. • kinase ... protein serine/threonine kinase activity. • GO:0001948 protein binding. • ATP binding. • Rho GTPase binding. ... Receptor protein serine/threonine kinase (EC 2.7.11.30). *Bone morphogenetic protein receptors *BMPR1 ... This GTPase cycles between an inactive GDP-bound form and an active GTP-bound form, and this RhoA flux seems important for ...
Calcium binds to proteins such as calmodulin (CaM) and an eye-specific protein kinase C (PKC) known as InaC. These proteins ... InaD contains five binding domains called PDZ domain proteins, which specifically bind the C termini of target proteins. ... In addition, proteins called arrestins bind metarhodopsin and prevent it from activating more Gq. A sodium-calcium exchanger ... TRP, InaC, and PLC form a signaling complex by binding a scaffolding protein called InaD. ...
Elevated intracellular Ca2+ also binds and allosterically activates proteins called calmodulins, which in turn go on to bind ... The GPCR can then activate an associated G protein by exchanging the GDP bound to the G protein for a GTP. The G protein's α ... binding of G proteins to receptors affects the receptor's affinity for ligands. Activated G proteins are bound to GTP. ... It is known that in the inactive state, the GPCR is bound to a heterotrimeric G protein complex. Binding of an agonist to the ...
Calmodulin binding molecule 8 Poly-A RNA contating poly (U) sequence 9 Glutathione GST fusion protein ... By using affinity chromatography, one can separate proteins that bind a certain fragment from proteins that do not bind that ... Monoclonal antibodies can also be selected to bind proteins with great specificity, where protein is released under fairly ... Proteins with a known affinity are protein tagged in order to aid their purification. The protein may have been genetically ...
GTP-binding protein regulators. *see GTP-binding protein regulators. Other. *Activating transcription factor 6 ... Membrane bound guanylate cyclases include an external ligand-binding domain (e.g., for peptide hormones such as BNP and ANP), a ... Guanylyl cyclase activator (protein). References[edit]. *^ Sakurai K.; Chen J.; Kefalov V. (2011). "Role of guanylate cylcase ... There are membrane-bound (type 1, guanylate cyclase-coupled receptor) and soluble (type 2, soluble guanylate cyclase) forms of ...
Calmodulin-binding proteins are, as their name implies, proteins which bind calmodulin. Examples include: Gap-43 protein ( ... Caldesmon Calmodulin-Binding Proteins at the US National Library of Medicine Medical Subject Headings (MeSH). ...
N3 is a protein fragment from the non-CaM binding region of a known CaM-binding protein (ATP2B1). (B) Full-length proteins, ... Many of the CaM-binding proteins identified belong to protein families such as the DEAD/H box proteins, ribosomal proteins, ... N1 (similar to cardiac morphogenesis protein) and N2 (MLL3 protein) are two protein fragments that do not bind to CaM in the ... A number of the CaM-binding proteins identified in this work belong to protein families such as the DEAD/H box proteins, ...
Calmodulin (CaM) is a major Ca2+ effector protein that binds up to four Ca2+ ions. CaM with bound Ca2+ can activate at least ... Ca2+/calmodulin-dependent protein kinase II;. CaM,. calmodulin;. CaM-NWT,. mutant CaM that binds Ca2+ only at N-terminal sites; ... Ca2+/calmodulin-dependent protein kinase II (CaMKII) is activated by calmodulin with two bound calciums. Julia M. Shifman, Mee ... Ca2+/calmodulin-dependent protein kinase II (CaMKII) is activated by calmodulin with two bound calciums ...
... pull-down assay is an effective way to investigate the interaction of CaM with various proteins. This method uses... ... While most target proteins bind Ca2+-CaM, certain proteins only bind to ApoCaM. Some bind CaM through their IQ-domain, ... Add protein loading buffer to all samples (i.e. homogenate, unbound and bound protein as well as those still bound to the beads ... Neurogranin (Ng) represents proteins that bind apo-CaM and CaMKII represents proteins that bind to Ca2+-rich CaM. CaM is shown ...
... Dev Biol. 2004 Oct 15;274(2):426-35. doi: 10.1016/j. ... Three protein-protein interaction domains were identified in enkurin: a C-terminal region is essential for channel interaction ... an IQ motif binds the Ca2+ sensor, calmodulin, in a Ca2+-dependent manner; and a proline-rich N-terminal region contains ... We identified a novel protein, enkurin, that is expressed at high levels in the testis and vomeronasal organ and at lower ...
A novel calmodulin-binding protein, belonging to the WD-repeat family, is localized in dendrites of a subset of CNS neurons. ... Striatin, a calmodulin-dependent scaffolding protein, directly binds caveolin-1. Gaillard, S., Bartoli, M., Castets, F., ... The polymerase (L) protein of rinderpest virus interacts with the host cell protein striatin. Sleeman, K., Baron, M.D. Virology ... Hence, it is likely that the proteins of the striatin family are addressed to membrane microdomains by their binding to ...
Addition of bovine brain calmodulin and S-100 inhibited Tetrahymena calmodulin-induced stimulation of guanylate cyclase, but ... Interaction of calcium-binding proteins with calmodulin-dependent guanylate cyclase in Tetrahymena plasma membrane Comp Biochem ... The inhibitory effects of brain calmodulin and S-100 were overcome by the addition of Tetrahymena calmodulin, but not by ... Addition of bovine brain calmodulin and S-100 inhibited Tetrahymena calmodulin-induced stimulation of guanylate cyclase, but ...
... binding proteins. In vitro binding studies have revealed multiple CaM-binding sites on TRPC proteins. Among them, a common CaM/ ... Multiple roles of calmodulin and other Ca(2+)-binding proteins in the functional regulation of TRP channels.. Zhu MX1. ... Likewise, multiple CaM-binding sites were found in other TRP proteins. These, together with the presence in close vicinity of ... Additional non-conserved CaM-binding sites are present at the amino and carboxyl termini of several TRPC proteins. ...
Calmodulin (CAM) pull-down-analysen er en effektiv måde at undersøge samspillet mellem Cam med forskellige proteiner. Denne ... Kaleka, K. S., Petersen, A. N., Florence, M. A., Gerges, N. Z. Pull-down of Calmodulin-binding Proteins. J. Vis. Exp. (59), ... Hathaway, D. R., Adelstein, R. S. Human platelet myosin light chain kinase requires the calcium-binding protein calmodulin for ... Identification and characterization of the calmodulin-binding domain of neuromodulin, a neurospecific calmodulin-binding ...
One domain of calmodulin is shown to be selective with binding involving short-distance interactions between hydrophobic ... The mechanisms behind the simultaneous specificity and variation of target protein binding is yet unknown but will aid ... while the other binds target proteins through a more flexible mechanism involving long-distance electrostatic interactions. ... Its two lobes bind calcium and allow the energetically unfavorable exposure of hydrophobic residues to the aqueous environment ...
Conserved Microsynteny of NPR1 with Genes Encoding a Signal Calmodulin-Binding Protein and a CK1-Class Protein Kinase in Beta ... "Conserved Microsynteny of NPR1 with Genes Encoding a Signal Calmodulin-Binding Protein and a CK1-Class Protein Kinase in Beta ...
Conserved Microsynteny of NPR1 with Genes Encoding a Signal Calmodulin-Binding Protein and a CK1-Class Protein Kinase in Beta ... encoding a chloroplast-targeted signal calmodulin-binding protein, and CK1PK, a CK1-class protein kinase. Conserved ... In this study, systematic DNA sequence analysis, gene annotation, and protein BLASTs were performed to determine genes near the ...
1991) A novel Ca2+/calmodulin-dependent protein kinase and a male germ cell-specific calmodulin-binding protein are derived ... Impaired Synaptic Plasticity and cAMP Response Element-Binding Protein Activation in Ca2+/Calmodulin-Dependent Protein Kinase ... Impaired Synaptic Plasticity and cAMP Response Element-Binding Protein Activation in Ca2+/Calmodulin-Dependent Protein Kinase ... Impaired Synaptic Plasticity and cAMP Response Element-Binding Protein Activation in Ca2+/Calmodulin-Dependent Protein Kinase ...
Cellular Distribution of Calmodulin and Calmodulin-Binding Proteins in Vicia faba L. Vincent Ling, Sarah M. Assmann ... The distribution of calmodulin (CaM) and CaM-binding proteins within Vicia faba was investigated. Both CaM and CaM-binding ... Cellular Distribution of Calmodulin and Calmodulin-Binding Proteins in Vicia faba L - December 01, 1992 ... Cellular Distribution of Calmodulin and Calmodulin-Binding Proteins in Vicia faba L. ...
Calmodulin (CaM) regulates plant disease responses through its downstream calmodulin-binding proteins (CaMBPs) often by ... The calmodulin-binding protein IQM1 interacts with CATALASE2 to affect pathogen defense. Tianxiao Lv, Xiaoming Li, Tian Fan, ... The calmodulin-binding protein IQM1 interacts with CATALASE2 to affect pathogen defense ... IQ-Motif Containing Protein 1), a Ca2+-independent CaMBP, in JA biosynthesis and defense against the necrotrophic pathogen ...
The interactions between guanine nucleotide regulatory proteins and the Ca(2+)-binding protein calmodulin were studied using ... Calmodulin binding distinguishes between βγ subunits of activated G proteins and transducin. L A Mangels, R R Neubig, H E Hamm ... Calmodulin binding distinguishes between βγ subunits of activated G proteins and transducin ... Calmodulin binding distinguishes between βγ subunits of activated G proteins and transducin ...
The Calmodulin Binding Domain Does Not Bind to Microtubules.. Protein (0.4 C) containing the calmodulin binding domain was ... Protein with the calmodulin binding domain alone (capable of binding to biotinylated calmodulin) failed to bind microtubules ( ... In a protein-protein interaction-based screening with calmodulin, we isolated a calmodulin binding protein (KCBP) from ... The motor protein containing the calmodulin binding domain did not bind microtubules in the presence of Ca2+-calmodulin (Figure ...
Neurogranin is a calmodulin-binding protein expressed exclusively in the brain, particularly in dendritic spines, and ... participating in the protein kinase C signaling pathway. Designed for immunohistochemistry, western blotting and related ... Neurogranin is a calmodulin-binding protein expressed exclusively in the brain, particularly in dendritic spines, and ... Neurogranin is the main postsynaptic protein regulating the availability of calmodulin, binding to it in the absence of calcium ...
Calmodulin Binding to the Polybasic C-Termini of STIM Proteins Involved in Store-Operated Calcium Entry.. Bauer, Mikael LU ; ... By binding STIM1 and STIM2, calmodulin may regulate store refilling, thereby ensuring the maintenance of its own action in ... By binding STIM1 and STIM2, calmodulin may regulate store refilling, thereby ensuring the maintenance of its own action in ... We show by isothermal titration calorimetry that calmodulin binds in a calcium-dependent manner to the polybasic C-termini of ...
Calmodulin from the Eucestoda and its use as a probe for calmodulin binding proteins. JANE L. BROOK, C. BRANFORD-WHITE, W.J. ... Calmodulin from the Eucestoda and its use as a probe for calmodulin binding proteins ... Calmodulin from the Eucestoda and its use as a probe for calmodulin binding proteins ... Calmodulin from the Eucestoda and its use as a probe for calmodulin binding proteins ...
"CALMODULIN AND CALMODULIN-BINDING PROTEINS IN PLANTS, Annual Review of Plant Biology" on DeepDyve, the largest online rental ... CALMODULIN AND CALMODULIN-BINDING PROTEINS IN PLANTS. CALMODULIN AND CALMODULIN-BINDING PROTEINS IN PLANTS Zielinski, Raymond E ... CALMODULIN AND CALMODULIN-BINDING PROTEINS IN PLANTS. Zielinski, Raymond E. Annual Review of Plant Biology. , Volume 49 (1) - ... Zielinski, R. (1998). CALMODULIN AND CALMODULIN-BINDING PROTEINS IN PLANTS. Annual Review of Plant Biology, 49(1), 697-725.. ...
The cDNA encoding the Trpl protein was initially isolated on the basis that the expressed protein binds calmodulin. Using both ... CBS-1 binds calmodulin in a Ca2+-dependent fashion, requiring Ca2+ concentrations above 0.3-0.5 μM for calmodulin binding. In ... Identification and characterization of two distinct calmodulin-binding sites in the Trpl ion-channel protein of Drosophila ... Identification and characterization of two distinct calmodulin-binding sites in the Trpl ion-channel protein of Drosophila ...
This family includes a unique kinesin with a calmodulin-binding domain referred to as kinesin-like calmodulin-binding protein ( ... Wargo et al., 2005)] to detect calmodulin binding. However, in both cases CrKCBP does not bind to calmodulin in either high or ... And finally, these differences in calmodulin binding may be due to the variability of the calmodulin-binding domain of CrKCBP ... calmodulin binding of CrKCBP or that our preparation of CrKCBP contains flagellar proteins that inhibit calmodulin binding. ...
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... and calmodulin-binding proteins. Title Cellular regulators. Title number Part A. Title part Calcium- and calmodulin-binding ... A. Calcum- and calmodulin-binding proteins -- pt. B. Calcum and lipids. Dimensions 24 cm.. Extent xxxi, 917 pages. Isbn ... A. Calcum- and calmodulin-binding proteins -- pt. B. Calcum and lipids. Dimensions 24 cm.. Extent xxxi, 917 pages. Isbn ... The item Cellular regulators, Part A, Calcium- and calmodulin-binding proteins, edited by Anthony R. Means, P. Michael Conn ...
... Academic Article ... Direct binding between CaM and FLIPL was demonstrated using recombinant proteins, and a CaM-binding region was identified in ... Furthermore, a 37.3 ± 5.7% increase (n = 6, P = 0.001) in CaM-FLIP binding was observed at 30 min after Fas stimulation, which ... Therefore we characterized the interaction of CaM with proteins recruited into the Fas-mediated DISC, including FADD (Fas- ...
In view of the role of calmodulin and calmodulin binding proteins in modulating the second messenger functions of Ca2+, we ... Affinity chromatography of amniotic fluid proteins revealed the presence of calmodulin binding proteins in samples obtained ... Culturing murine splenocytes in presence of the isolated calmodulin binding proteins (10 micrograms protein/10(6) cells) ... Full text: Available Index: IMSEAR (South-East Asia) Main subject: Calmodulin-Binding Proteins / Female / Humans / Pregnancy / ...
... ... In recent years, a variety of calcium/calmodulin-binding proteins with unique regulatory controls have been identified that ... Herein, for the first time two plant calcium/calmodulin-binding transcription factors, GT2-like protein (AtGT2L) and ... It has been well documented that calcium/calmodulin signaling plays important roles in plant growth, development and responses ...
All three genes are functional and encode the exact same CaM protein. Moreover, CaM has a completely conserved amino acid ... Biochemical studies have demonstrated differential effects on Ca2+ binding affinities for these CaM variants. Moreover, CaM ... Calmodulin (CaM) senses local changes in Ca2+ concentration and relays the information to numerous interaction partners. The ... All three genes are functional and encode the exact same CaM protein. Moreover, CaM has a completely conserved amino acid ...
phosphatidylinositol phosphate binding. • phosphatidylserine binding. • calmodulin binding. • protein binding. Cellular ... Growth Associated Protein 43 also known as GAP43 is a protein that in humans is encoded by the GAP43 gene.[5] ... neuron growth-associated protein 43. Function[edit]. GAP43, is a nervous tissue-specific cytoplasmic protein that can be ... protein kinase C-activating G-protein coupled receptor signaling pathway. • response to wounding. • regulation of growth. • ...
  • Striatin, a putative protein phosphatase 2A (PP2A) B-type regulatory subunit, is a multi-domain scaffolding protein that has recently been linked to several diseases including cerebral cavernous malformation (CCM), which causes symptoms ranging from headaches to stroke. (biomedcentral.com)
  • Excessive elevation of intracellular Ca 2+ levels and, subsequently, hyperactivation of Ca 2+ /calmodulin-dependent processes might play an important role in the pathologic events following cerebral ischemia. (fujita-hu.ac.jp)
  • Taken together, these results provide evidence that PEP-19 overexpression enhances resistance to Ca 2+ -mediated cytotoxicity, which might be mediated through calmodulin inhibition, and also raises the possibility that PEP-19 degradation by calpain might produce an aberrant activation of calmodulin functions, which in turn causes neuronal cell death. (fujita-hu.ac.jp)
  • Deletion of the caveolin-binding domain on striatin abolishes striatin family oligomerization and PP2A binding. (biomedcentral.com)
  • PEP-19 is a neuronally expressed polypeptide that acts as an endogenous negative regulator of calmodulin by inhibiting the association of calmodulin with enzymes and other proteins. (fujita-hu.ac.jp)
  • Currently, much effort is devoted toward studying gene, and hence protein, function and regulation by analyzing mRNA expression profiles, gene disruption phenotypes, two-hybrid interactions, and protein subcellular localization ( 2 ). (sciencemag.org)
  • A novel calmodulin-binding protein, belonging to the WD-repeat family, is localized in dendrites of a subset of CNS neurons. (wikigenes.org)
  • The microarrays can also be used to screen protein-drug interactions and to detect posttranslational modifications. (sciencemag.org)
  • As there are 6 TIR1/AUXIN SIGNALING F-BOX proteins (AFBs) and 29 Aux/IAA proteins in Arabidopsis thaliana, combinatorial interactions may result in many co-receptors with distinct auxin-sensing properties. (ipb-halle.de)
  • In addition to binding the PP2A A/C heterodimer to form a PP2A holoenzyme, striatin associates with cerebral cavernous malformation 3 (CCM3) protein, the mammalian Mps one binder (MOB) homolog, Mob3/phocein, the mammalian sterile 20-like (Mst) kinases, Mst3, Mst4 and STK25, and several other proteins to form a large signaling complex. (biomedcentral.com)
  • We also demonstrate that the AFB5Aux/IAA co-receptor selectively binds the auxinic herbicide picloram. (ipb-halle.de)
  • To facilitate studies of the yeast proteome, we cloned 5800 open reading frames and overexpressed and purified their corresponding proteins. (sciencemag.org)
  • The proteins were printed onto slides at high spatial density to form a yeast proteome microarray and screened for their ability to interact with proteins and phospholipids. (sciencemag.org)
  • We have constructed a yeast proteome microarray containing approximately 80% yeast proteins and screened it for a number of biochemical activities. (sciencemag.org)
  • The yeast proteins are fused to glutathione S -transferase-polyhistidine (GST-HisX6) at their NH 2 -termini and expressed in yeast using the inducible GAL1 promoter ( 5 , 9 ). (sciencemag.org)
  • The proteins were expressed in yeast to help ensure that the proteins were modified and folded properly. (sciencemag.org)
  • Heterologous experiments in yeast and quantitative IAA binding assays using purified proteins showed that different combinations of TIR1 and Aux/IAA proteins form co-receptor complexes with a wide range of auxin-binding affinities. (ipb-halle.de)
  • Two distinct regions of striatin are capable of stably binding directly or indirectly to Mob3--one N-terminal, including the coiled-coil domain, and another more C-terminal, including the WD-repeat domain. (biomedcentral.com)
  • PP2A associates with the coiled-coil domain of striatin, but unlike Mob3 and Mst3, its binding appears to require striatin oligomerization. (biomedcentral.com)
  • It binds Mst3 likely as a dimer with CCM3 via residues lying between striatin's calmodulin-binding and WD-domains and recruits the PP2A A/C heterodimer to its coiled-coil/oligomerization domain. (biomedcentral.com)
  • Mob3 can associate with striatin sequences C-terminal to the Mst3 binding site but also with sequences proximal to striatin-associated PP2A, consistent with a possible role for Mob 3 in the regulation of Mst3 by PP2A. (biomedcentral.com)
  • In contrast to the reduction in PEP-19, no changes in calmodulin occurred following excitotoxicity, suggesting the loss of negative regulation of calmodulin by PEP-19. (fujita-hu.ac.jp)
  • A daunting task after a genome has been fully sequenced is to understand the functions, modification, and regulation of every encoded protein ( 1 ). (sciencemag.org)
  • Expression of PEP-19 in HEK293T cells suppressed calmodulin-dependent signaling and protected against cell death elicited by Ca 2+ ionophore. (fujita-hu.ac.jp)
  • Major hurdles in screening an entire proteome array have been the ability to generate the necessary expression clones and also the expression and purification of proteins in a high-throughput fashion. (sciencemag.org)
  • We found that the biological activity of (-)-JA-L-Ile is unexpectedly low compared to coronatine and the synthetic isomer (+)-JA-L-Ile, which suggests that the stereochemical orientation of the cyclopentanone-ring side chains greatly affects receptor binding. (ipb-halle.de)
  • Here we show that efficient auxin binding requires assembly of an auxin co-receptor complex consisting of TIR1 and an Aux/IAA protein. (ipb-halle.de)
  • In principle, the biochemical activities of proteins can be systematically probed by producing proteins in a high-throughput fashion and analyzing the functions of hundreds or thousands of protein samples in parallel using protein microarrays ( 5 , 6 , 8 ). (sciencemag.org)
  • The kinesin-like calmodulin binding protein (KCBP) is a new member of the kinesin superfamily that appears to be present only in plants. (plantcell.org)
  • Kinesin-like calmodulin-binding protein, KCBP, is a novel member of the C-kinesin superfamily first discovered in flowering plants. (biologists.org)
  • This family includes a unique kinesin with a calmodulin-binding domain referred to as kinesin-like calmodulin-binding protein (KCBP). (biologists.org)
  • Calmodulin acts by binding to short peptide sequences within target proteins, thereby inducing structural changes, which alters their activities in response to changes in intracellular Ca 2+ concentration. (deepdyve.com)
  • It seems likely that calmodulin performs diverse functions by interacting with many different target proteins. (genetics.org)
  • The results reveal new details of how calmodulin binds and regulates its target proteins. (innovations-report.com)
  • The results Rief and biophysicist Jan Philipp Junker report in the early edition of PNAS show that their approach also enables detailed comparative studies of binding sequences for different target proteins. (innovations-report.com)
  • CaM is a multifunctional intermediate messenger protein that transduces calcium signals by binding calcium ions and then modifying its interactions with various target proteins. (moleculardevices.com)
  • Calmodulin (CaM) is an essential Ca 2+ -binding protein that transduces intracellular Ca 2+ signals into cellular effects by binding to target proteins ( Chin and Means, 2000 ). (biologists.org)
  • Enkurin interacts with several TRPC proteins (TRPC1, TRPC2, TRPC5, but not TRPC3) and colocalizes with these channels in sperm. (nih.gov)
  • Interacts with protein phosphatase 2A (PP2A) (Potential). (genecards.org)
  • Interacts with calmodulin. (abcam.com)
  • Calmodulin binds to calcium, undergoing a conformational change, and then interacts with enzyme-molecules. (google.com)
  • Moreover, RalA specifically interacts with Exo84 and Sec5 to regulate transport of membrane proteins in polarized epithelial cells and GLUT4 to the plasma membrane, as well as mitochondrial fission for cell division. (wikipedia.org)
  • We purified the part of the calcium pump that interacts with calcium-activated calmodulin, and we managed to crystallise a protein complex. (ku.dk)
  • I am looking at how calmodulin interacts with CaMKII in the presence of calcium," he said. (uh.edu)
  • Phage display has been used to isolate CaM-binding sequences from short synthetic peptide libraries, but the selected CaM-binding sequences were poorly correlated with naturally occurring proteins ( 17 ). (pnas.org)
  • Peptide with sequence C-KIKSGERGRKG, from the internal region of the Neurogranin precursor protein sequence according to NP_006167.1. (neuromics.com)
  • Using both fusion proteins and a synthetic peptide, we now show that two calmodulin-binding sites are present in the C-terminal domain of the Trpl protein, CBS-1 and CBS-2. (biochemj.org)
  • Professor Matthias Rief and colleagues at the Technische Universitaet Muenchen had previously shown that they could fix a single calmodulin molecule between a surface and the cantilever tip of a specially built atomic-force microscope, expose it to calcium ions in solution, induce peptide binding and unbinding, and measure changes in the molecule's mechanical properties as it did its work. (innovations-report.com)
  • By applying mechanical force," Junker says, "we are able to dismantle the calmodulin-target peptide complex with surgical precision. (innovations-report.com)
  • For single-step purification of calmodulin-binding peptide fusion proteins.Purification of calmodulin-regulated proteins from all eukaryotic cells. (bio-medicine.org)
  • We show here that recombinant, purified nNOSμ, despite the peptide insertion, has the same spectroscopic properties, l -arginine k cat and K m values, optimal pH, and calmodulin binding affinity constant as nNOSα. (aspetjournals.org)
  • This novel isoform is slightly larger than the neuronal nNOSα because of an alternative splicing that inserts a 34 amino acid peptide into the protein sequence between the CaM- and FMN-binding domains of the protein. (aspetjournals.org)
  • This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca++-dependent inhibition of smooth muscle contraction. (wikipedia.org)
  • C almodulin (CaM) is a small ubiquitously expressed EF-hand Ca 2+ -binding protein that mediates a wide variety of cellular functions in eukaryotes ( 1 - 7 ). (pnas.org)
  • Cellular Distribution of Calmodulin and Calmodulin-Binding Proteins in Vicia faba L. (plantphysiol.org)
  • Kinesins and kinesin-like proteins (KLPs), a family of microtubule motor proteins, are involved in many dynamic cellular processes, such as transport of vesicles and organelles, nuclear fusion, spindle formation, and chromosome movement during cell division ( Bloom and Endow, 1994 ). (plantcell.org)
  • CALMODULIN AND CALMODULIN-BINDING PROTEINS IN PLANTS Zielinski, Raymond E. 1998-06-01 00:00:00 ▪ Abstract Calmodulin is a small Ca 2+ -binding protein that acts to transduce second messenger signals into a wide array of cellular responses. (deepdyve.com)
  • The item Cellular regulators, Part A, Calcium- and calmodulin-binding proteins, edited by Anthony R. Means, P. Michael Conn represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in Boston University Libraries . (bu.edu)
  • Phylogenetic analyses indicate that the CaM-binding site is conserved in peroxisomal processing proteases of higher plants (dicots, monocots) but not present in orthologs of animals or cellular slime molds. (deepdyve.com)
  • Apart from one whole day focused on the fascinating roles played by calmodulin in cellular activities, the other sessions were devoted to calmodulin-related calcium binding proteins in muscle and non- muscle tissues and to some selected biological systems such as mitochondria, secretory cells or sarcoplasmic reticulum in which calcium also plays a crucial role. (springer.com)
  • This protein is involved in many cellular processes such as glycogen metabolism, cytoskeletal control, neurotransmission, phosphate activity and control of NAD + /NADP + ratios. (sigmaaldrich.com)
  • Cellular calcium changes can be sensed and interpreted by calcium-binding proteins that function as signal sensors [ 20 ]. (pubmedcentralcanada.ca)
  • The interaction of CaM with a specific target is determined not only by the Ca 2+ -dependent affinity of calmodulin but also by the proximity to that target in the cellular environment. (biologists.org)
  • The interaction of CaM with its binding targets is determined not only by its affinity for them in the presence or absence of Ca but also by its proximity to those targets in the cellular environment. (biologists.org)
  • Chaperones are critical for the folding and regulation of a wide array of cellular proteins. (chemweb.com)
  • David Kuykendall, Jonathan Shao, and Tammy Murphy, "Conserved Microsynteny of NPR1 with Genes Encoding a Signal Calmodulin-Binding Protein and a CK1-Class Protein Kinase in Beta vulgaris and Two Other Eudicots," International Journal of Plant Genomics , vol. 2008, Article ID 391259, 8 pages, 2008. (hindawi.com)
  • Microsynteny was discovered for NPR1 with genes CaMP , encoding a chloroplast-targeted signal calmodulin-binding protein, and CK1PK , a CK1-class protein kinase. (hindawi.com)
  • CaM with bound Ca 2+ can activate at least six signaling enzymes in the spine. (pnas.org)
  • PEP-19 is a neuronally expressed polypeptide that acts as an endogenous negative regulator of calmodulin by inhibiting the association of calmodulin with enzymes and other proteins. (fujita-hu.ac.jp)
  • The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme-substrate-calmodulin-Ca 2+ complexes are particularly stable. (sigmaaldrich.com)
  • This protein is found in all eukaryotic cells, where it regulates and modifies the activities of many calcium-binding enzymes. (sciencephoto.com)
  • The protein has a reversible effect on Ca 2+ -induced activation and inhibition of many enzymes in both the liver and renal cortex cells [ PMID: 1315924 ]. (ebi.ac.uk)
  • Calmodulin (CaM) is a calcium-binding messenger protein expressed in all eukaryotic cells. (moleculardevices.com)
  • Filamin A (FlnA) is a ubiquitous actin binding protein which anchors various transmembrane proteins to the cell cytoskeleton and provides a scaffold to many cytoplasmic signaling proteins involved in actin cytoskeleton remodeling in response to mechanical stress and cytokines stimulation. (chemweb.com)
  • GAP43, is a nervous tissue-specific cytoplasmic protein that can be attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4. (wikipedia.org)
  • GAP43, the consensus choice for its designation, [9] is a nervous system-specific protein that is attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4, though it can exist in the non-bound form in the cytoplasm . (wikipedia.org)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • Among the detailed insights this approach made accessible are the hierarchy of folding and target binding, the sequence of unbinding events, and target-specific differences in terms of what is called cooperative binding. (innovations-report.com)
  • CaBP4 protein sequence, tissue distribution and immunolocalization. (nih.gov)
  • 1. A method for increasing stem and tuber elongation in a potato plant relative to a control plant, comprising the steps of introducing a nucleic acid construct into a potato plant cell and growing the cell into a plant, wherein the nucleic acid construct comprises a nucleotide sequence that encodes the protein of Sequence Listing ID No. 2. (google.com)
  • 5. A transgenic potato plant comprising a nucleic acid construct, wherein the nucleic acid construct comprises a nucleotide sequence that encodes the protein of Sequence Listing ID No. 2, and wherein the nucleotide sequence is isolated from nature or is synthetic. (google.com)
  • The Ral isoforms share an 80% overall match in amino acid sequence and 100% match in their effector-binding region. (wikipedia.org)
  • The sequence for Calmodulin is shown below. (whozoo.org)
  • Calmodulin, which mediates many calcium-driven metabolic reactions, is found in all eukaryotes and its sequence is remarkably conserved. (whozoo.org)
  • Moreover the calmodulin sequence is itself a variant of the calcium-binding protein muscle troponin C. (whozoo.org)
  • The Ca 2+ /calmodulin-dependent protein kinase type IV/Gr (CaMKIV/Gr) is of particular interest in neuronal Ca 2+ signaling because of its expression profile and its function as a transcriptional activator. (jneurosci.org)
  • Estrogen receptor α (ERα) is a modular protein of the steroid/nuclear receptor family of transcriptional regulators that upon binding to the hormone undergoes structural changes, resulting in its nuclear translocation and docking to specific chromatin sites. (mcponline.org)
  • So far, the conventional SDS/PAGE gel overlay with recombinant, radiolabeled CaM is still the most commonly used method for the identification and characterization of CaM-binding proteins, although it is time-consuming and the number of CaM-binding proteins it can reveal is limited ( 11 , 15 ). (pnas.org)
  • The inducer binding site of the E. coli lactose repressor has been examined by in vitro characterization of mutant proteins with amino acid substitutions at the hypothetical inducer binding site (Sams, C. F., Vyas, N. K. (rice.edu)
  • We report here heterologous expression of nNOSμ in Escherichia coli and purification and characterization of the protein. (aspetjournals.org)
  • To distinguish whether the binding of purified beta gamma subunits to calmodulin was unique to brain beta gamma or to the method of purification, similar experiments were performed using transducin. (biochemj.org)
  • Calmodulin, an EF-hand Calcium 2 + -binding protein, undergoes a conformational shift when binding Calcium 2 +, exposing several hydrophobic residues. (asahq.org)
  • Troponin C shows little effect on the cyclase activity regardless of the presence or absence of Tetrahymena calmodulin. (nih.gov)
  • Both calmodulins from Tetrahymena and bovine brain elicited stimulation of heart phosphodiesterase, while troponin C and S-100 did not affect the phosphodiesterase activity in the presence and absence of Tetrahymena calmodulin. (nih.gov)
  • Calmodulin-like protein AtCML3 mediates dimerization of peroxisomal processing protease AtDEG15. (deepdyve.com)
  • F92A) is synthetically lethal with a mutation in MYO2 that encodes a class V unconventional myosin with calmodulin-binding domains. (genetics.org)
  • that she studied encodes a protein called calmodulin. (coursera.org)
  • The major components of the cytoskeleton are considered, contractile proteins, actin-myosin, microtubule-dynein and intermediate filaments. (nii.ac.jp)
  • However, at present, there are no selective small-molecule inhibitors available for this protein kinase. (ox.ac.uk)
  • Search proteins in UniProtKB for this molecule. (uniprot.org)
  • Methods developed by biophysicists at the Technische Universitaet Muenchen (TUM) have enabled them to manipulate and observe calmodulin in action, on the single-molecule scale. (innovations-report.com)
  • Measuring the force needed to bend the calmodulin molecule out of its stable condition at any given moment enables the researchers to compute the energies associated with binding both the calcium ions and the amino acid chains. (innovations-report.com)
  • Original paper: "Single-molecule force spectroscopy distinguishes target binding modes of calmodulin," by Jan Philipp Junker and Matthias Rief, published in the online Early Edition of PNAS, Proceedings of the National Academy of Sciences for the week of August 10, 2009. (innovations-report.com)
  • Molecule model of the calcium-binding protein calmodulin (CaM). (sciencephoto.com)
  • immunogen = raised against a recombinant protein corresp. (alzforum.org)
  • Results: The presence of volatile anesthetics altered the Calcium 2 + -binding affinity of calmodulin in a dose-dependent fashion. (asahq.org)
  • At 0.57% (0.25 mM) halothane and 1.7% (0.66 mM) isoflurane, the affinity of calmodulin for Calcium 2 + relative to control was decreased. (asahq.org)
  • In the CNS, proteins of the Munc13 family are critical in regulating neurotransmitter release and synaptic plasticity. (jneurosci.org)
  • Essential role of Ca2+-binding protein 4, a Cav1.4 channel regulator, in photoreceptor synaptic function. (nih.gov)
  • g ) Colocalization of synaptic vesicle proteins SV2 and synaptophysin (green) with mouse CaBP4 (red). (nih.gov)
  • Enzyme assays confirmed that most of these compounds are equipotent inhibitors of both human CAMKKs and isothermal titration calorimetry (ITC) revealed that binding to some of these molecules to CAMKK2 is enthalpy driven. (ox.ac.uk)
  • These sites are exposed after a conformational change induced by the action of Ca 2+ on separate Ca 2+ -binding sites. (sigmaaldrich.com)
  • Chelating agents strip Ca 2+ from the calmodulin, reversing the conformational change that exposed the protein binding sites. (sigmaaldrich.com)
  • I'll be looking at the conformational change of proteins," Ezerski said. (uh.edu)
  • In vitro binding studies have revealed multiple CaM-binding sites on TRPC proteins. (nih.gov)
  • This minus-end-directed kinesin exhibits Ca 2+ -calmodulin-sensitive motor activity in vitro and has been implicated in trichome morphogenesis and cell division. (biologists.org)
  • Indeed, a large number of calmodulin-binding proteins possessing diverse activities in vitro have been identified so far ( C ohen and K lee 1988 ). (genetics.org)
  • 1) Dynamic features of microtubules in vitro and the modification by Maps protein and dynein ATP-ase, visualized under a dark-field microscope. (nii.ac.jp)
  • 2) Translocation of microtubules and actin filaments in vitro driven by rotational motor proteins, dynein, myosin, and kinesin. (nii.ac.jp)
  • Calmodulin (CaM) is a major Ca 2+ effector protein that binds up to four Ca 2+ ions. (pnas.org)
  • In fluctuating cytosolic Ca 2+ , a large fraction of free CaM is bound to fewer than four Ca 2+ ions. (pnas.org)
  • Thus, initial binding of CaM to a target may depend on the target's affinity for CaM with only one or two bound Ca 2+ ions. (pnas.org)
  • We show that CaM with two Ca 2+ ions bound in its C-terminal lobe not only binds to CaMKII with low micromolar affinity but also partially activates kinase activity. (pnas.org)
  • When CaM with two Ca 2+ ions bound to either the N- or the C-terminal lobe associates with a target, the affinity of the remaining lobe for Ca 2+ increases, often by at least one order of magnitude, causing high positive cooperativity of Ca 2+ binding ( 5 ). (pnas.org)
  • When there are many calcium ions in a cell, some of these bind to calmodulin, which is thereby able to activate the calcium pump. (ku.dk)
  • With two calmodulin-binding domains in the calcium pump, the cell can adjust the transportation to be energy efficient, at the same time as being able to quickly reduce the number of calcium ions if the concentration approaches a toxic level," Dr Poulsen concludes. (ku.dk)
  • This fellowship, which can be renewed for two additional years, will support Ezerski's research on modeling how the proteins calmodulin and Calmodulin-Kinase-II (CaMKII) change shape to fit each other as a result of calcium signaling. (uh.edu)
  • Additional non-conserved CaM-binding sites are present at the amino and carboxyl termini of several TRPC proteins. (nih.gov)
  • Ca 2+ /CaM can bind to many different protein targets with high affinity ( 8 - 11 ). (pnas.org)
  • Intriguingly, protein targets of CaM continue to be discovered ( 10 , 11 , 14 - 16 ). (pnas.org)
  • A key obstacle to studies on Ca 2+ -sensor proteins is the difficulty in identifying their downstream targets because of the technical limitations of various methods that have been used, especially on a proteome-wide scale. (pnas.org)
  • Binding to targets increases the affinity of CaM's remaining Ca 2+ -binding sites. (pnas.org)
  • This sensitivity is possible because association of CaM with its targets stabilizes its Ca 2+ -bound conformation and, therefore, increases its affinity for Ca 2+ ( 2 - 4 ). (pnas.org)
  • Because of this property, we predict that when Ca 2+ concentrations fluctuate in the cytosol, competition among targets for binding of CaM will depend on their affinities for CaM with one or two bound Ca 2+ and also on the magnitude of cooperativity induced by binding to each target. (pnas.org)
  • Many of these proteins have been well characterized biochemically, but it has generally remained unclear which calmodulin targets have functional significance and how these functions are regulated in the cell. (genetics.org)
  • Two essential calmodulin targets have thus far been identified in yeast. (genetics.org)
  • demonstrated that the availability of CaM to interact with low-affinity CaM-binding targets is dynamically regulated and raised the question of the source of the CaM made available by PKC activation in smooth-muscle cells. (biologists.org)
  • Employing the mRNA display technique, we have scanned the human proteome for CaM-binding proteins and have identified and characterized a large number of both known and previously uncharacterized proteins that interact with CaM in a Ca 2+ -dependent manner. (pnas.org)
  • Each group has a characteristic functional defect in actin organization ( cmd1A ), calmodulin localization ( cmd1B ), nuclear division ( cmd1C ), or bud emergence ( cmd1D ). (genetics.org)
  • These domains include (i) CG-1 DNA-binding domain containing a predicted bipartite nuclear localization signal (NLS) at the N-terminus. (nature.com)
  • These stains are used to detect the presence, abundance, and localization of specific proteins to aid in determining the direction of differentiation in neoplasms with similar morphology as well as to provide prognostic or therapeutic information, among other applications. (aruplab.com)
  • Therefore, new methodologies are necessary to identify the downstream binding partners of CaM. (pnas.org)
  • Previous studies have shown that calcium pumps in both animals and plants work together with a protein called calmodulin. (ku.dk)
  • Previous studies have shown that calcium/calmodulin-regulated SR/CAMTAs are important for modulation of disease resistance, cold sensitivity and wounding response in vegetative tissues. (pubmedcentralcanada.ca)
  • Addition of bovine brain calmodulin and S-100 inhibited Tetrahymena calmodulin-induced stimulation of guanylate cyclase, but they did not affect enzymatic activity in the presence of calcium alone. (nih.gov)
  • The effect was independent of the type of bovine brain G protein (Go/Gi, Gs), method of activation and the presence of magnesium. (biochemj.org)
  • Bovine testicular calmodulin coupled to Sepharose 4B by the CNBr method. (sigmaaldrich.com)
  • In comparison, similar incorporation of the radiolabel into lymphocytes obtained from cord blood was enhanced only by 2 fold in presence of calmodulin binding proteins , though at a much lower protein concentration (50 ng/10(6) cells ). (bvsalud.org)
  • Protein concentration is above or equal to 0.05 mg/ml. (abcam.com)
  • With increasing Ca21 concentration, Ca21-CaM first binds to and displaces high-affinity CaMBS1 before even higher Ca21 concentration leads to displacement of CaMBS2 from the catalytic core, allowing free movement of the A domain as required for ion pumping. (ku.dk)
  • The activities of these two isoforms are regulated by the Ca 2+ -concentration because they bind CaM in a Ca 2+ -dependent manner. (aspetjournals.org)
  • Protein concentration was determined using a colorimetric assay. (creativebiomart.net)
  • The CAMTAs are a family of well-characterized Calmodulin (CaM) binding transcription factors that are reported to be evolutionarily conserved from plants to animals 1 . (nature.com)
  • Members of this family share a common domain structure that consists of a transcription activation domain, a DNA-binding domain, and a calmodulin-binding domain. (genecards.org)
  • The intrinsic tyrosine fluorescence of calmodulin was measured at an excitation wavelength of 280 nm and an emission wavelength of 320 nm. (asahq.org)
  • The KCBP is unique in its ability to interact with calmodulin in a Ca 2 -dependent manner. (plantcell.org)
  • May interact with calmodulin. (genecards.org)
  • Although the vast majority of FlnA binding partners interact with the carboxy-terminal immunoglobulin like (Igl) repeats of FlnA, little is known on the role of the amino-N-terminal repeats. (chemweb.com)
  • Depending on how many of these sites are occupied affects calmodulin's shape and its ability to interact with other proteins, one of which is CaMKII. (uh.edu)
  • Ezerski's research will focus on modeling how these three molecules - calcium, calmodulin, and CaMKII - interact with each other. (uh.edu)
  • The older idea of how proteins interact with each other was the lock-and-key mechanism, which said that one protein has one shape and the other has another shape and if they're compatible, then they'll lock together. (uh.edu)