A 13.2-kDa member of the S-100 family of calcium-binding proteins that can form homo- or heterocomplexes with CALGRANULIN A and a variety of other proteins. The calgranulin A/B heterodimer is known as LEUKOCYTE L1 ANTIGEN COMPLEX. Calgranulin B is expressed at high concentrations in GRANULOCYTES during early monocyte differentiation, and serum calgranulin B levels are elevated in many inflammatory disorders such as CYSTIC FIBROSIS.
A 10.8-kDa member of the S-100 family of calcium-binding proteins that can form homo- or heterocomplexes with CALGRANULIN B and a variety of other proteins. The calgranulin A/B heterodimer is known as LEUKOCYTE L1 ANTIGEN COMPLEX. Calgranulin A is found in many cell types including GRANULOCYTES; KERATINOCYTES; and myelomonocytes, and has been shown to act as a chemotactic substance for NEUTROPHILS. Because it is present in acute inflammation but absent in chronic inflammation, it is a useful biological marker for a number of pathological conditions.
A family of highly acidic calcium-binding proteins found in large concentration in the brain and believed to be glial in origin. They are also found in other organs in the body. They have in common the EF-hand motif (EF HAND MOTIFS) found on a number of calcium binding proteins. The name of this family derives from the property of being soluble in a 100% saturated ammonium sulfate solution.
A member of the S-100 protein family that is present at high levels in the blood and interstitial fluid in several infectious, inflammatory, and malignant disorders, including rheumatoid arthritis, inflammatory bowel disease, and cystic fibrosis. It is a complex of a light chain (CALGRANULIN A) and a heavy chain (CALGRANULIN B). L1 binds calcium through an EF-hand motif, and has been shown to possess antimicrobial activity.
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A transmembrane-protein belonging to the TNF family of intercellular signaling proteins. It is a widely expressed ligand that activates APOPTOSIS by binding to TNF-RELATED APOPTOSIS-INDUCING LIGAND RECEPTORS. The membrane-bound form of the protein can be cleaved by specific CYSTEINE ENDOPEPTIDASES to form a soluble ligand form.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.
The valve between the left ventricle and the ascending aorta which prevents backflow into the left ventricle.
A pathological process consisting of hardening or fibrosis of an anatomical structure, often a vessel or a nerve.
Enlargement of the LEFT VENTRICLE of the heart. This increase in ventricular mass is attributed to sustained abnormal pressure or volume loads and is a contributor to cardiovascular morbidity and mortality.
Pathological conditions involving any of the various HEART VALVES and the associated structures (PAPILLARY MUSCLES and CHORDAE TENDINEAE).
A pathological constriction that can occur above (supravalvular stenosis), below (subvalvular stenosis), or at the AORTIC VALVE. It is characterized by restricted outflow from the LEFT VENTRICLE into the AORTA.
Products derived from the nonenzymatic reaction of GLUCOSE and PROTEINS in vivo that exhibit a yellow-brown pigmentation and an ability to participate in protein-protein cross-linking. These substances are involved in biological processes relating to protein turnover and it is believed that their excessive accumulation contributes to the chronic complications of DIABETES MELLITUS.
Pathologic deposition of calcium salts in tissues.
20-carbon saturated monocarboxylic acids.
A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
A genus of deer, Rangifer, that inhabits the northern parts of Europe, Asia, and America. Caribou is the North American name; reindeer, the European. They are often domesticated and used, especially in Lapland, for drawing sleds and as a source of food. Rangifer is the only genus of the deer family in which both sexes are antlered. Most caribou inhabit arctic tundra and surrounding arboreal coniferous forests and most have seasonal shifts in migration. They are hunted extensively for their meat, skin, antlers, and other parts. (From Webster, 3d ed; Walker's Mammals of the World, 5th ed, p1397)
A subfamily of HERPESVIRIDAE characterized by a short replication cycle. The genera include: SIMPLEXVIRUS; VARICELLOVIRUS; MAREK'S DISEASE-LIKE VIRUSES; and ILTOVIRUS.
EPIDEMIOLOGIC STUDIES based on the detection through serological testing of characteristic change in the serum level of specific ANTIBODIES. Latent subclinical infections and carrier states can thus be detected in addition to clinically overt cases.
The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)
Exclusive legal rights or privileges applied to inventions, plants, etc.
Traumatic injuries to the brain, cranial nerves, spinal cord, autonomic nervous system, or neuromuscular system, including iatrogenic injuries induced by surgical procedures.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
Acute and chronic (see also BRAIN INJURIES, CHRONIC) injuries to the brain, including the cerebral hemispheres, CEREBELLUM, and BRAIN STEM. Clinical manifestations depend on the nature of injury. Diffuse trauma to the brain is frequently associated with DIFFUSE AXONAL INJURY or COMA, POST-TRAUMATIC. Localized injuries may be associated with NEUROBEHAVIORAL MANIFESTATIONS; HEMIPARESIS, or other focal neurologic deficits.
Penetrating and non-penetrating injuries to the spinal cord resulting from traumatic external forces (e.g., WOUNDS, GUNSHOT; WHIPLASH INJURIES; etc.).
Damage inflicted on the body as the direct or indirect result of an external force, with or without disruption of structural continuity.
Proteins and peptides found in SALIVA and the SALIVARY GLANDS. Some salivary proteins such as ALPHA-AMYLASES are enzymes, but their composition varies in different individuals.
Tumors or cancer of the MOUTH.
Polyketides of up to a few dozen carbons in length, formed by chain extension of multiple PROPIONATES and oxygenated to form tetrahydrofuran and lactone rings along the length of the chain. They are found in ANNONACEAE and other PLANTS. Related compounds cyclize to MACROLIDES.
Pathological processes involving the PERIODONTIUM including the gum (GINGIVA), the alveolar bone (ALVEOLAR PROCESS), the DENTAL CEMENTUM, and the PERIODONTAL LIGAMENT.
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
An essential ribonucleoprotein reverse transcriptase that adds telomeric DNA to the ends of eukaryotic CHROMOSOMES.
Products of viral oncogenes, most commonly retroviral oncogenes. They usually have transforming and often protein kinase activities.
Insertion of viral DNA into host-cell DNA. This includes integration of phage DNA into bacterial DNA; (LYSOGENY); to form a PROPHAGE or integration of retroviral DNA into cellular DNA to form a PROVIRUS.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A predominantly X-linked recessive syndrome characterized by a triad of reticular skin pigmentation, nail dystrophy and leukoplakia of mucous membranes. Oral and dental abnormalities may also be present. Complications are a predisposition to malignancy and bone marrow involvement with pancytopenia. (from Int J Paediatr Dent 2000 Dec;10(4):328-34) The X-linked form is also known as Zinsser-Cole-Engman syndrome and involves the gene which encodes a highly conserved protein called dyskerin.
Proteins obtained from ESCHERICHIA COLI.
A calcium-binding protein that is 92 AA long, contains 2 EF-hand domains, and is concentrated mainly in GLIAL CELLS. Elevation of S100B levels in brain tissue correlates with a role in neurological disorders.
Proteins prepared by recombinant DNA technology.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Antibodies produced by a single clone of cells.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.

Oxidation regulates the inflammatory properties of the murine S100 protein S100A8. (1/297)

The myeloid cell-derived calcium-binding murine protein, S100A8, is secreted to act as a chemotactic factor at picomolar concentrations, stimulating recruitment of myeloid cells to inflammatory sites. S100A8 may be exposed to oxygen metabolites, particularly hypochlorite, the major oxidant generated by activated neutrophils at inflammatory sites. Here we show that hypochlorite oxidizes the single Cys residue (Cys41) of S100A8. Electrospray mass spectrometry and SDS-polyacrylamide gel electrophoresis analysis indicated that low concentrations of hypochlorite (40 microM) converted 70-80% of S100A8 to the disulfide-linked homodimer. The mass was 20,707 Da, 92 Da more than expected, indicating additional oxidation of susceptible amino acids (possibly methionine). Phorbol 12-myristate 13-acetate activation of differentiated HL-60 granulocytic cells generated an oxidative burst that was sufficient to efficiently oxidize exogenous S100A8 within 10 min, and results implicate involvement of the myeloperoxidase system. Moreover, disulfide-linked dimer was identified in lung lavage fluid of mice with endotoxin-induced pulmonary injury. S100A8 dimer was inactive in chemotaxis and failed to recruit leukocytes in vivo. Positive chemotactic activity of recombinant Ala41S100A8 indicated that Cys41 was not essential for function and suggested that covalent dimerization may structurally modify accessibility of the chemotactic hinge domain. Disulfide-dependent dimerization may be a physiologically significant regulatory mechanism controlling S100A8-provoked leukocyte recruitment.  (+info)

Cell type and gene-specific activity of the retinoid inverse agonist AGN 193109: divergent effects from agonist at retinoic acid receptor gamma in human keratinocytes. (2/297)

Retinoids are important regulators of epithelial differentiation. AGN 193109 is a high-affinity antagonist and inverse agonist for the nuclear retinoic acid receptors (RARs). Paradoxically, both AGN 193109 and retinoid agonists inhibit the expression of the differentiation marker MRP-8 in normal human keratinocytes (NHKs). TTNPB, an RAR agonist, and AGN 193109 mutually antagonize MRP-8 inhibition at both mRNA and protein levels. We find that this antagonism, which is greatest at an AGN 193109:TTNPB ratio of about 10:1, is absent when either compound is in significant excess. The potent RARalpha-specific agonist, AGN 193836, has no effect on MRP-8 regulation. These data indicate that inverse agonists and agonists suppress MRP-8 in NHKs through RARgamma using distinct and mutually inhibitory mechanisms. The activity of AGN 193109 on MRP-8 is cell type specific. In differentiating ECE16-1 cervical cells, TTNPB inhibits while AGN 193109 induces MRP-8 mRNA levels. The effect of AGN 193109 on genes inhibited by retinoid agonists in NHKs is also selective; expression of the differentiation markers transglutaminase 1 and keratin 6 is not down-regulated by AGN 193109 whereas stromelysin-1 expression is suppressed. These results show a complex gene and cell context-specific interplay between agonist and inverse agonist for the regulation of gene expression.  (+info)

A null mutation in the inflammation-associated S100 protein S100A8 causes early resorption of the mouse embryo. (3/297)

S100A8 (also known as CP10 or MRP8) was the first member of the S100 family of calcium-binding proteins shown to be chemotactic for myeloid cells. The gene is expressed together with its dimerization partner S100A9 during myelopoiesis in the fetal liver and in adult bone marrow as well as in mature granulocytes. In this paper we show that S100A8 mRNA is expressed without S100A9 mRNA between 6.5 and 8. 5 days postcoitum within fetal cells infiltrating the deciduum in the vicinity of the ectoplacental cone. Targeted disruption of the S100A8 gene caused rapid and synchronous embryo resorption by day 9. 5 of development in 100% of homozygous null embryos. Until this point there was no evidence of developmental delay in S100A8-/- embryos and decidualization was normal. The results of PCR genotyping around 7.5-8.5 days postcoitum suggest that the null embryos are infiltrated with maternal cells before overt signs of resorption. This work is the first evidence for nonredundant function of a member of the S100 gene family and implies a role in prevention of maternal rejection of the implanting embryo. The S100A8 null provides a new model for studying fetal-maternal interactions during implantation.  (+info)

S100A12 is expressed exclusively by granulocytes and acts independently from MRP8 and MRP14. (4/297)

Changes in cytosolic calcium concentrations regulate a wide variety of cellular processes, and calcium-binding proteins are the key molecules in signal transduction, differentiation, and cell cycle control. S100A12, a recently described member of the S100 protein family, has been shown to be coexpressed in granulocytes and monocytes together with two other S100 proteins, MRP8 (S100A8) and MRP14 (S100A9), and a functional relationship between these three S100 proteins has been suggested. Using Western blotting, calcium overlays, intracellular flow cytometry, and cytospin preparations, we demonstrate that S100A12 expression in leukocytes is specifically restricted to granulocytes and that S100A12 represents one of the major calcium-binding proteins in these cells. S100A12, MRP8, and MRP14 translocate simultaneously from the cytosol to cytoskeletal and membrane structures in a calcium-dependent manner. However, no evidence for direct protein-protein interactions of S100A12 with either MRP8 or MRP14 or the heterodimer was found by chemical cross-linking, density gradient centrifugation, mass spectrometric measurements, or yeast two hybrid detection. Thus, S100A12 acts individually during calcium-dependent signaling, independent of MRP8, MRP14, and the heterodimer MRP8/MRP14. This granulocyte-specific signal transduction pathway may offer attractive targets for therapeutic intervention with exaggerated granulocyte activity in pathological states.  (+info)

S100A8: emerging functions and regulation. (5/297)

The functional importance of members of the S100 Ca2+-binding protein family is becoming apparent. Murine (m)S100A8 (initially named CP-10) is a potent chemoattractant (10(-13) to 10(-11) M) for myeloid cells and the chemotactic activity of other S100s has since been reported, suggesting a new class of chemoattractants. Murine S100A8 has been associated with a number of acute and chronic inflammatory conditions including bacterial infection, atherogenesis, and cystic fibrosis. It is expressed constitutively with S100A9 in neutrophils and is regulated by inflammatory stimulants in macrophages and microvascular endothelial cells. The lack of co-expression of S100A9 with S100A8 in activated macrophages suggests distinct functions for the proteins expressed by different cell types. Glucocorticoids up-regulate induction of mS100A8 by inflammatory mediators, and its exquisite sensitivity to oxidation suggests that it may protect against oxidative tissue damage. Inactivation of the mS100A8 gene is embryonic lethal, providing the first evidence for non-redundant function of a member of the S100 gene family. S100A8 may have an immunoregulatory role by contributing to the regulation of fetal-maternal interactions. It may play a protective role and its absence may allow infiltration by maternal cells, a process eventually manifesting as resorption. This review focuses on the variety of emerging functions attributed to murine S100A8, a protein implicated in embryogenesis, growth, differentiation, and immune and inflammatory processes.  (+info)

Calcium-induced noncovalently linked tetramers of MRP8 and MRP14 detected by ultraviolet matrix-assisted laser desorption/ionization mass spectrometry. (6/297)

MRP8 and MRP14 are members of the S100 family of calcium-binding proteins which play an important role during calcium-induced activation of phagocytes. Both proteins form noncovalently associated complexes as a prerequisite for biological functions. The exact stoichiometric composition of these complexes, however, has not been completely clarified yet. In the present study we show for the first time by ultraviolet matrix-assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) the calcium-induced formation of noncovalently associated (MRP8/MRP14)2 tetramers. Furthermore, we could determine posttranslational modifications of MRP8 and MRP14, the stoichiometric proportion of the two known MRP14 isoforms in the complexes as well as the number of calcium ions bound to the single MRP8 and MRP14 monomers and tetramers. MRP14 showed a higher affinity for calcium than MRP8. Upon complex formation the calcium binding increased to maximal saturation of the known EF hands in the complexed forms. Calcium-induced stabilization of the MRP8/MRP14 complexes was confirmed by DSC studies. Our results extend scope and application of UV-MALDI-MS by allowing identification of noncovalent protein complexes, the identification of minor alterations of subunits in such complexes as well as the determination of bound calcium ions.  (+info)

The two calcium-binding proteins, S100A8 and S100A9, are involved in the metabolism of arachidonic acid in human neutrophils. (7/297)

Recently, we identified the two myeloid related protein-8 (MRP8) (S100A8) and MRP14 (S100A9) as fatty acid-binding proteins (Klempt, M., Melkonyan, H., Nacken, W., Wiesmann, D., Holtkemper, U., and Sorg, C. (1997) FEBS Lett. 408, 81-84). Here we present data that the S100A8/A9 protein complex represents the exclusive arachidonic acid-binding proteins in human neutrophils. Binding and competition studies revealed evidence that (i) fatty acid binding was dependent on the calcium concentration; (ii) fatty acid binding was specific for the protein complex formed by S100A8 and S100A9, whereas the individual components were unable to bind fatty acids; (iii) exclusively polyunsaturated fatty acids were bound by S100A8/A9, whereas saturated (palmitic acid, stearic acid) and monounsaturated fatty acids (oleic acid) as well as arachidonic acid-derived eicosanoids (15-hydroxyeicosatetraenoic acid, prostaglandin E(2), thromboxane B(2), leukotriene B(4)) were poor competitors. Stimulation of neutrophil-like HL-60 cells with phorbol 12-myristate 13-acetate led to the secretion of S100A8/A9 protein complex, which carried the released arachidonic acid. When elevation of intracellular calcium level was induced by A23187, release of arachidonic acid occurred without secretion of S100A8/A9. In view of the unusual abundance in neutrophilic cytosol (approximately 40% of cytosolic protein) our findings assign an important role for S100A8/A9 as mediator between calcium signaling and arachidonic acid effects. Further investigations have to explore the exact function of the S100A8/A9-arachidonic acid complex both inside and outside of neutrophils.  (+info)

Zinc binding reverses the calcium-induced arachidonic acid-binding capacity of the S100A8/A9 protein complex. (8/297)

Analysis of the calcium-induced arachidonic acid (AA) binding to S100A8/A9 revealed that maximal AA binding was achieved at molar ratios of 1 mol S100A8 and 1 mol S100A9 and for values greater than 3 calciums per EF-hand. The AA binding capacity was not induced by the binding of other bivalent cations, such as Zn2+, Cu2+, and Mg2+, to the protein complex. In contrast, the binding of AA was prevented by the addition of either Zn2+ or Cu2+ in the presence of calcium, whereas Mg2+ failed to abrogate the AA binding capacity. The inhibitory effect was not due to blocking the formation of S100A8/A9 as demonstrated by a protein-protein interaction assay. Fluorescence measurements gave evidence that both Zn2+ and Cu2+ induce different conformational changes thereby affecting the calcium-induced formation of the AA binding pocket within the protein complex. Due to the fact that the inhibitory effect of Zn2+ was present at physiological serum concentrations, it is assumed that released S100A8/A9 may carry AA at inflammatory lesions, but not within the blood compartment.  (+info)

S100 calcium-binding protein A9 (S100A9) also known as migration inhibitory factor-related protein 14 (MRP14) or calgranulin B is a protein that in humans is encoded by the S100A9 gene. The proteins S100A8 and S100A9 form a heterodimer called calprotectin. S100-A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase. MRP14 complexes with MRP-8 (S100A8), another member of the S100 family of calcium-modulated proteins; together, MRP8 and MRP14 regulate myeloid cell function by binding to Toll-like receptor 4 (TLR4) and the receptor for advanced glycation end products. Altered expression of the S100A9 protein is ...
In this work, we describe novel gadolinium containing designer nanoprobes displaying antibodies against Mrp-8/14 to target inflammation in a murine model of atherosclerosis. Molecular probes targeting atherosclerosis-associated moieties have been widely used in research settings.16-19 The challenge has been to identify suitable ligands that simultaneously provide sufficient selectivity and high levels of expression and serve in a pathophysiologic context so that ligation of the target results in neutral or even beneficial effects on the disease process. Inflammation-associated calgranulins, S100A8 (Mrp8) and S100A9 (Mrp14) are upregulated following activation in response to cell contact with activated endothelium.8,20,21 Mrp-14 forms a heterodimeric complex with Mrp-8 and is isolated almost exclusively in the dimeric form (Mrp).1,3-5 Mrp-14 is functionally homologous across species, is highly expressed in atherosclerosis, and participates in amplification of inflammation, providing a ...
The calcium-binding, migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The…
Clone REA917 recognizes the calcium- and zinc-binding protein S100A8, also known as migration inhibitory factor-related protein 8 (MRP-8), calprotectin, or calgranulin-A. S100A8 is mainly expressed in myeloid cells, e.g., monocytes, macrophages, and granulocytes and upregulated in different tumors, e.g., in skin, breast, colon, pancreatic, bladder, and ovarian malignancies. S100A8 is involved in the regulation of inflammatory processes and immune response and plays a role in antimicrobial, cytostatic, and chemotactic activities.Additional information: Clone REA917 displays negligible binding to Fc receptors. - Norge
CAGBMigration inhibitory factor-related protein 14; Calgranulin B; calgranulin-B; Calprotectin L1H subunit; CFAGMRP-14,60B8AG; CGLB; L1AG; LIAG; MAC387; MIF; MRP-14; MRP14Leukocyte L1 complex heavy chain; NIF; P14; protein S100-A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100A9 ...
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Using transcriptional profiling of platelets from patients presenting with acute myocardial infarction, we identified myeloid-related protein-14 (MRP-14, also known as S100A9) as an acute myocardial infarction gene and reported that platelet MRP-14 binding to platelet CD36 regulates arterial thrombosis. However, whether MRP-14 plays a role in venous thrombosis is unknown. We subjected WT and Mrp-14-deficient (Mrp-14-/-) mice to experimental models of deep vein thrombosis (DVT) by stasis ligation or partial flow restriction (stenosis) of the inferior vena cava. Thrombus weight in response to stasis ligation or stenosis was reduced significantly in Mrp-14-/- mice compared with WT mice. The adoptive transfer of WT neutrophils or platelets, or the infusion of recombinant MRP-8/14, into Mrp-14-/- mice rescued the venous thrombosis defect in Mrp-14-/- mice, indicating that neutrophil- and platelet-derived MRP-14 directly regulate venous thrombogenesis. Stimulation of neutrophils with MRP-14 induced ...
The p38 MAPK pathway participates in a number of neutrophil functions critical to generation and regulation of the inflammatory response, including chemotaxis, adherence, respiratory burst activity, degranulation, and cytoskeletal reorganization (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12). Understanding the molecular mechanisms by which p38 MAPK participates in these responses is hindered by the limited number of targets of p38 MAPK identified to date in neutrophils. MAPKAPK2 and p47phox are the only clearly identified p38 MAPK targets in human neutrophils (1, 9, 10, 21). A previous study by Lewis et al. (45) determined that 20 of 25 ERK targets identified in a global screen were not previously known. Thus, it is likely that a number of important targets of p38 MAPK that participate in regulation of neutrophil responses remain to be identified. The goal of the present study was to apply a recently developed proteomic approach that allows simultaneous identification of multiple substrates of a single ...
Oligosaccharides are increasingly being recognized as important mediators of signaling in innate and adaptive immune responses (59, 60, 61, 62, 63). Considerable diversity of oligosaccharide structures provides enormous potential for information display on cell surfaces and specific recognition by different lectins. Glycans that have the same structure can also have different functions depending upon the proteins and cell types that carry them. Examples are the selectin ligands that mediate both inflammation-initiated leukocyte rolling as well as physiological lymphocyte homing and recirculation. However, not all glycan structures in mammals have been proven, much less functionally characterized. We earlier identified a family of novel carboxylated glycans on endothelial cells and macrophages that mediate inflammation. Here, we show that interfering with the interaction between these glycans and their putative lectin partners using a monoclonal anti-glycan Ab prevents the pathogenic process in a ...
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BioVendor - BioVendor Research and Diagnostic Products is a developer and manufacturer of immunoassays, recombinant proteins, antibodies and endotoxin-removal products.
A structurally diverse group of endogenous molecules that are multifunctional, having physiological functions inside the cell, but when released from dying cells or from cells under stress or certain immune cells, they function to activate INNATE IMMUNITY. Uncontrolled and excessive release of alarmins may contribute to INFLAMMATION; CARCINOGENESIS, and NEOPLASM METASTASIS. Alarmins are also critical for heart and nerve tissue homeostasis. . ...
Schnitzlers syndrome (SchS) is a disabling autoinflammatory disorder, characterized by a chronic urticarial rash, an M-protein, arthralgia, and other signs of systemic inflammation. Anti-interleukin-1 (IL-1) beta antibodies are highly effective, but the pathophysiology is still largely unknown. Here we studied the effect of in-vivo IL-1 inhibition on serum markers of inflammation and cellular immune responses. Eight patients with SchS received monthly subcutaneous (s.c.) injections with 150 mg canakinumab for six months. Blood was drawn for measurement of serum markers of inflammation (12 times per patient) and for functional and phenotypic analysis of both freshly isolated and toll-like receptor (TLR)-ligand-stimulated peripheral blood mononuclear cells (PBMCs) (five times per patient). All data were compared to results of healthy controls. IL-6 levels in serum and in lysates of freshly isolated PBMCs and serum myeloid-related protein (MRP8)/14 and S100A12 levels correlated with disease activity. In
From NCBI Gene:. This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]. From UniProt: ...
PAA114Mu01, Polyclonal Antibody to Placenta Growth Factor (PLGF), 胎盘生长因子(PLGF)多克隆抗体, PlGF2; PGF; PGFL; Placental Growth Factor-Like; Vascular Endothelial Growth Factor-Related Protein | 仅供体外研究使用,不用于临床诊断!请索取进口关税税单及报关单!
HDGFRP2 - HDGFRP2 (untagged)-Human hepatoma-derived growth factor-related protein 2 (HDGFRP2), transcript variant 2 available for purchase from OriGene - Your Gene Company.
In this study, we characterized mice that were deficient in the S100 protein MRP-14 (S100A9). Although there was normal expression of MRP-8 mRNA in the MRP-14−/− myeloid cells, surprisingly, no MRP-8 protein was present. The absence of MRP-8 protein could be due to inefficient translation of MPR-8 mRNA or, more likely, due to instability of MRP-8 protein in the absence of its partner, MRP-14. This finding contrasts with evidence that murine MRP-14 and MRP-8 (CP-10) exist separately in myeloid cells (28, 40) and that CP-10 alone can function as a potent chemotactic factor (28, 29). In addition, immunohistochemical studies of mouse tissues show that MRP-14 (this study) and MRP-8 (data not shown) are coexpressed in myeloid cells. In support of this finding, the heterodimer can be isolated from spleen (data not shown) and bone marrow (13). Information from physical studies with the human proteins indicates that MRP-8 and MRP-14 form a heterodimer more readily than either forms a homodimer and ...
BÜHLMANN is a world leader in calprotectin and offers a range of assays as diagnostic tools to determine the inflammatory disease process.
MRP8小鼠单克隆抗体[MRP8 7C12/4](ab20220)可与人样本反应并经WB, ELISA, IHC, Flow Cyt实验严格验证,被1篇文献引用。所有产品均提供质保服务,中国75%以上现货。
MRP4兔多克隆抗体(ab32550)可与大鼠, 人样本反应并经WB实验严格验证,被2篇文献引用。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
in Clinical Chemistry (2008), 54. BACKGROUND: SELDI-TOF mass spectrometry (MS) is a high-throughput proteomic approach with potential for identifying novel forms of serum biomarkers of arthritis. METHODS: We used SELDI-TOF MS to analyze ... [more ▼]. BACKGROUND: SELDI-TOF mass spectrometry (MS) is a high-throughput proteomic approach with potential for identifying novel forms of serum biomarkers of arthritis. METHODS: We used SELDI-TOF MS to analyze serum samples from patients with various forms of inflammatory arthritis. Several protein profiles were collected on different Bio-Rad Laboratories ProteinChip arrays (CM10 and IMAC-Cu(2+)) and were evaluated statistically to select potential biomarkers. RESULTS: SELDI-TOF MS analyses identified several calgranulin proteins [S100A8 (calgranulin A), S100A9 (calgranulin B), S100A9*, and S100A12 (calgranulin C)], serum amyloid A (SAA), SAA des-Arg (SAA-R), and SAA des-Arg/des-Ser (SAA-RS) as biomarkers and confirmed the results with other techniques, ...
Our previous studies illustrated that deletion of RAGE was protective in murine models of long-term diabetes-associated neuropathy (14,15). Because RAGE is critically involved in inflammatory mechanisms by virtue of its ability to bind members of the S100/calgranulin family, HMGB1 and Mac-1 (19,20,36), we tested its role in superimposed acute nerve crush injury. These studies bear clinical relevance because diabetic subjects often sustain thermal and other acute injuries to their extremities during advancing neuropathy (7-10). This present work reveals that RAGE, particularly in bone marrow cells and in diabetes, contributes to maladaptive inflammatory mechanisms after acute nerve crush.. Our data confirmed that diabetes was associated with increased expression of AGEs in the peripheral nerve in the basal state (15) and buttressed our findings that AGE levels were lower in diabetic RAGE-null mice compared with diabetic WT mice (37). We previously showed that RAGE suppresses mRNA and protein ...
Human S100A7 Induces Mature Interleukin1α Expression by RAGE-p38 MAPK-Calpain1 Pathway in Psoriasis. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
References for Abcams Recombinant Human S100 alpha 2 protein (ab104821). Please let us know if you have used this product in your publication
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Ops I meant CRP Are these different measures and tests or are they the same thing? ETA: ok I realize now that they are different. My CRP is .7 (within...
BACKGROUND/OBJECTIVES: Obesity is related to a dynamic extracellular matrix (ECM) remodeling, which involves the synthesis and degradation of different proteins, such as tenascin C (TNC) in the adipose tissue (AT). Given the functional relationship between leptin and inducible nitric oxide synthase (iNOS), our aim was to analyze the impact of the absence of the iNOS gene in AT inflammation and ECM remodeling in ob/ob mice. SUBJECTS/METHODS: The expression of genes involved in inflammation and ECM remodeling was evaluated in 10-week-old male double knockout (DBKO) mice simultaneously lacking the ob and iNOS genes as well as in ob/ob mice classified into three groups [control, leptin-treated (1 mg kg -1 day -1 ) and pair-fed ...
COLLECTION OF FAECAL SAMPLES | Sampling of faecal calprotectin; general adviceIn proctitis the area of inflamed mucosa is very limited and the contact time…
Our results indicate a steady decline in calprotectin concentration in the first 6 days after stool collection in samples kept at room temperature. A similar slope was seen in stool extracts kept at room temperature, while the stability of calprotectin was preserved in samples stored in a refrigerator at 4°C. We did not test the superiority of one ELISA kit over another. For that, we would have needed to perform a multitude of tests. The decline in calprotectin concentration over time was observed regardless of the ELISA kit used, which makes inadvertently smoothening of the curve unlikely.. Literature on calprotectin stability under various preservation conditions is scarce. A Swedish group observed a decline in calprotectin when stool samples were stored at room temperature for 7 days but claimed that the concentration remained unchanged in the first 3 days after collection.4 A Spanish group claimed that calprotectin remained stable in stool extracts stored at room temperature for 4 days,5 as ...
0083] 1. Davies J R, Rudd J H, Weissberg P L. Molecular and metabolic imaging of atherosclerosis. J Nucl Med. 2004; 45:1898-1907. [0084] 2. Kislinger T, Fu C, Huber B, Qu W, Taguchi A, Yan S D, Hofmann M, Yan S F, Pischetsrieder M, Stern D, Schmidt A M. N.sup.ε-(carboxymethyl) lysine adducts of proteins are ligands for receptor for advanced glycation endproducts that activate cell signaling pathways and modulate gene expression. J Biol Chem. 1999; 274:31740-31749. [0085] 3. Hofmann M A, Drury S, Fu C, Qu W, Taguchi A, Lu Y, Avila C, Kambham N, Bierhaus A, Nawroth P, Neurath M F, Slattery T, Beach D, McClary J, Nagashima M, Morser J, Stern D, Schmidt A M. RAGE mediates a novel proinflammatory axis: a central cell surface receptor for S100/calgranulin polypeptides. Cell. 1999; 97:889-901. [0086] 4. Schmidt A M, Yan S D, Brett J, Mora R, Nowygrod R, Stern D. Regulation of human mononuclear phagocyte migration by cell surface binding proteins for AGE. J. Clin. Invest. 1993; 91:2155-2168. [0087] 5. ...
0175]1. Hugel B, Martinez M C, Kunzelmann C, Freyssinet J M. Membrane microparticles: two sides of the coin. Physiology (Bethesda, Md. February 2005; 20:22-27. [0176]2. Mallat Z, Benamer H, Hugel B, Benessiano J, Steg P G, Freyssinet J M, Tedgui A. Elevated levels of shed membrane microparticles with procoagulant potential in the peripheral circulating blood of patients with acute coronary syndromes. Circulation. Feb. 29 2000; 101(8):841-843. [0177]3. Nieuwland R, Berckmans R J, Rotteveel-Eijkman R C, Maquelin K N, Roozendaal K J, Jansen P G, ten Have K, Eijsman L, Hack C E, Sturk A. Cell-derived microparticles generated in patients during cardiopulmonary bypass are highly procoagulant. Circulation. Nov. 18 1997; 96(10):3534-3541. [0178]4. Tesse A, Martinez M C, Hugel B, Chalupsky K, Muller C D, Meziani F, Mitolo-Chieppa D, Freyssinet J M, Andriantsitohaina R. Upregulation of proinflammatory proteins through NF-kappaB pathway by shed membrane microparticles results in vascular hyporeactivity. ...
Calprotectin is released by white blood cells (neutrophils) in the digestive tract with inflammation. Calprotectin tests measure levels in stool to help detect conditions such as inflammatory bowel disease (IBD) and infections.
Semantic Scholar extracted view of Dynamic changes in calprotectin and its correlation with traditional markers of oxidative stress in patients with acute ischemic stroke. by Antonios Chatzopoulos et al.
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When asking the question of carboxylated vs. non-carboxylated, the answer comes down to an adhesion coating controlled by carboxylation.
Kit Component:- KN203070G1, MRPS18A gRNA vector 1 in pCas-Guide vector- KN203070G2, MRPS18A gRNA vector 2 in pCas-Guide vector- KN203070D, donor…
複合型酸化的リン酸化異常(COXPD)は、ミトコンドリアの酸化的リン酸化システムの欠陥により起こる多様な症状を持つ疾患群である。リボソームタンパクの遺伝子(MRPS16 and MRPS22)の変異は、出生前に重症な小児病を引き起こすことが報告されている。また、COXPD患者のミトコンドリアの翻訳伸長因子の遺伝子(GFM1, TUFM, TSFM およびC12orf65)に変異があることも報告されている ...
Objective: To study the active involvement of S100A8 and A9 in joint inflammation and cartilage destruction during antigen-induced arthritis (AIA).. Methods: Joint inflammation and cartilage destruction was measured with 99mTc uptake and histology. The role of S100A8/A9 was investigated by inducing AIA in S100A9-/- mice, which lacks also S100A8 at protein level, or after intra-articular injection of rS100A8 in mouse kneejoints. Cartilage destruction was measured using immunolocalisation of the neoepitope VDIPEN or NITEGE. mRNA levels of MMPs and cytokines were measured using RT-PCR.. Results: Immunization of S100A9-/- mice with the antigen mBSA induced normal cellular and humoral responses, not different from WT controls. However, joint swelling measured at day 3 and 7 after AIA induction was significantly lower (36 and 70% respectively). Histologically, at day 7 AIA, cellular mass was much lower (63- 80%) and proteoglycan depletion from cartilage layers was significantly reduced (between ...
One of the most definitive examples of a vertebrate extraorganismal structural protein can be found in three-spined sticklebacks (Gasterosteus aculeatus). In the breeding male the kidney hypertrophies and synthesizes an adhesive protein called spiggin, which is secreted into the urinary bladder from where it is employed as a structural thread for nest building. This paper describes the first molecular characterization of spiggin and demonstrates that this adhesive is a protein complex assembled from a potential of three distinct subunits (alpha, beta, and gamma). These subunits arise by alternative splicing, and 11-ketoandrogens induce their expression in stickleback kidneys. Analysis of the predicted amino acid sequence of each subunit reveals a modular organization whose structural elements display a similarity to the multimerization domains found within von Willebrand Factor-related proteins. These results implicate that spiggin utilizes a conserved multimerization mechanism for the ...
Inflammatory mediators trigger polymorphonuclear neutrophils (PMN) to produce reactive oxygen species (ROS: O2-, H2O2, ∙OH). Mediated by myeloperoxidase in PMN, HOCl is formed, detectable in a chemiluminescence (CL) assay. We have shown that the abundant cytosolic PMN protein calprotectin (S100A8/A9) similarly elicits CL in response to H2O2 in a cell-free system. Myeloperoxidase and calprotectin worked synergistically. Calprotectin-induced CL increased, whereas myeloperoxidase-triggered CL decreased with pH | 7.5. Myeloperoxidase needed NaCl for CL, calprotectin did not. 4-hydroxybenzoic acid, binding ∙OH, almost abrogated calprotectin CL, but moderately increased myeloperoxidase activity. The combination of native calprotectin, or recombinant S100A8/A9 proteins, with NaOCl markedly enhanced CL. NaOCl may be the synergistic link between myeloperoxidase and calprotectin. Surprisingly- and unexplained- at higher concentration of S100A9 the stimulation vanished, suggesting a switch from pro-oxidant to
Division of Chemical Biology and Medicinal Chemistry. Researching the COVID-19 spike protein that creates infection in a host by binding to its cell receptors. Recent scientific reporting suggests heparin sulfate interacts with the COVID-19 spike protein, and the Liu group seeks to determine which specific heparin sulfate structure displays the highest affinity to COVID-19 spike protein. Heparin sulfate is also known to attenuate inflammation induced by a COVID-19 infection - a significant outcome in many infected patients that causes uncontrolled inflammation responses in the lung, leading to lung failure. Using heparin sulfate, Lius team will seek to inhibit a series of proinflammatory proteins released after COVID-19 infection to reduce symptoms in patients.. Learn more about the Jian Liu Lab ...
Head and neck squamous cell carcinoma express high levels of the EF-hand calcium-binding protein S100A2 in contrast to other tumorigenic tissues and cell lines where the expression of this protein is reduced. Subtractive hybridization of tumorigenic versus normal tumor-derived mammary epithelial cells has previously identified the S100A2 protein as potential tumor suppressor. The biological function of S100A2 in carcinogenesis, however, has not been elucidated to date. Here, we report for the first time that during recovery from hydroxyurea treatment, the S100A2 protein translocated from the cytoplasm to the nucleus and co-localized with the tumor suppressor p53 in two different oral carcinoma cells (FADU and SCC-25). Co-immunoprecipitation experiments and electrophoretic mobility shift assay showed that the interaction between S100A2 and p53 is Ca(2+)-dependent. Preliminary characterization of this interaction indicated that the region in p53 involved with binding to S100A2 is located at the C ...
Results qPCR analysis showed increased expression of the alarmins S100A8/A9 and several members of the canonical Wnt signaling pathway in the CIOA model at all time points measured. In the DMM model, the expression of S100A8/A9 and Wnt16 and WISP1 was mainly increased at day 28 after induction. Kinetics of S100 and Wnt expression were comparable, as was observed in both models. This gave rise to the question if an interrelationship existed between these factors. Therefore, we overexpressed Wnt8a and Wnt16, two canonical Wnts, with the use of adenoviral vectors. However, this did not result in increased expression of S100A8 and S100A9, both on RNA and protein level. In contrast, we found that injection of S100A8 increased the expression of Wnt16 in the synovium and accumulation of β-catenin, a hallmark of canonical Wnt signaling, in both cartilage and synovium. In addition, the downstream mediator of canonical Wnt signaling WISP1, was increased. Furthermore, we found reduced β-catenin ...
Calprotectin (CP) is an antimicrobial protein produced and released by neutrophils that inhibits the growth of pathogenic microorganisms by sequestering essential metal nutrients in the extracellular space. In this work, spectroscopic and thermodynamic metal-binding studies are presented to delineate the zinc-binding properties of CP. Unique optical absorption and EPR spectroscopic signatures for the interfacial His3Asp and His4 sites of human calprotectin are identified by using Co(II) as a spectroscopic probe. Zinc competition titrations employing chromophoric Zn(II) indicators provide a 2:1 Zn(II):CP stoichiometry, confirm that the His[subscript 3]Asp and His[subscript 4] sites of CP coordinate Zn(II), and reveal that the Zn(II) affinity of both sites is calcium-dependent. The calcium-insensitive Zn(II) competitor ZP4 affords dissociation constants of K[subscript d1] = 133 ± 58 pM and K[subscript d2] = 185 ± 219 nM for CP in the absence of Ca(II). These values decrease to K[subscript d1] ...
Calprotectin is a 36kDa calcium and zinc binding protein expressed by the gene S100 calcium-binding protein A8, S100A8. It accounts for 30 to 40% of neutrophils cytosol. In vitro studies show it has bacteriostatic and fungistatic properties. It is...
Recombinant human S100B Calcium-Binding Protein, Western Blot Control protein - 230-00002-WBC. Liquid . Expression system is Escherichia coli (E.coli).
Calgranulin Brophy MB, Nolan EM (March 2015). "Manganese and microbial pathogenesis: sequestration by the Mammalian immune ...
Other names for calprotectin include MRP8-MRP14, calgranulin A and B, cystic fibrosis antigen, L1, 60BB antigen, and 27E10 ...
Other endogenous inhibitors include calgranulin (an S-100 calcium binding protein), Tamm-Horsfall protein, glycosaminoglycans, ... Considering its extremely high levels of inhibition of growth and aggregation of calcium oxalate crystals, calgranulin might be ... some researchers have found that calgranulin, a protein formed in the kidney, is a potent inhibitor of the in vivo formation of ...
Ankylosing spondylitis: A version of the HLA-B gene called HLA-B27 increases the risk of developing ankylosing spondylitis. It is uncertain how HLA-B27 causes this increased risk. Researchers speculate that HLA-B27 may abnormally display to the immune system peptides that trigger arthritis. Other research suggests that joint inflammation characteristic of this disorder may result from improper folding of the HLA-B27 protein or the presence of abnormal forms of the protein on the cell surface. Although most patients with ankylosing spondylitis have the HLA-B27 variation, many people with this particular variation never develop the disorder. Other genetic and environmental factors are likely to affect the chances of developing ankylosing spondylitis and influence its progression. HLA-B27 is associated with the spondyloarthropathies, a group of disorders that includes ankylosing spondylitis and other inflammatory joint diseases. Some of these diseases are associated with a common skin condition ...
The human leukocyte antigen (HLA) system or complex is a gene complex encoding the major histocompatibility complex (MHC) proteins in humans. These cell-surface proteins are responsible for the regulation of the immune system in humans. The HLA gene complex resides on a 3 Mbp stretch within chromosome 6p21. HLA genes are highly polymorphic, which means that they have many different alleles, allowing them to fine-tune the adaptive immune system. The proteins encoded by certain genes are also known as antigens, as a result of their historic discovery as factors in organ transplants. Different classes have different functions:. HLAs corresponding to MHC class I (A, B, and C) present peptides from inside the cell. For example, if the cell is infected by a virus, the HLA system brings fragments of the virus to the surface of the cell so that the cell can be destroyed by the immune system. These peptides are produced from digested proteins that are broken down in the proteasomes. In general, these ...
GAP43, is a nervous tissue-specific cytoplasmic protein that can be attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4. This sequence targets GAP43 to lipid rafts. It is a major protein kinase C (PKC) substrate and is considered to play a key role in neurite formation, regeneration, and plasticity.[7][8] The role of GAP-43 in CNS development is not limited to effects on axons: It is also a component of the centrosome, and differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. As a consequence, in the cerebellum, the neuronal precursor pool fails to expand normally and the cerebellum is significantly smaller.. Several different laboratories studying the same protein, now called GAP43, initially used different names. It was designated F1, then B-50, then GAP43, pp46, and finally neuromodulin, each name reflecting a different function of the same molecule.[9] F1 was ...
The peptide translocation from the cytosol into the lumen of the ER is accomplished by the transporter associated with antigen processing (TAP). TAP is a member of the ABC transporter family and is a heterodimeric multimembrane-spanning polypeptide consisting of TAP1 and TAP2. The two subunits form a peptide binding site and two ATP binding sites that face the cytosol. TAP binds peptides on the cytoplasmic side and translocates them under ATP consumption into the lumen of the ER. The MHC class I molecule is then, in turn, loaded with peptides in the lumen of the ER. The peptide-loading process involves several other molecules that form a large multimeric complex called the Peptide loading complex[7] consisting of TAP, tapasin, calreticulin, calnexin, and Erp57 (PDIA3). Calnexin acts to stabilize the class I MHC α chains prior to β2m binding. Following complete assembly of the MHC molecule, calnexin dissociates. The MHC molecule lacking a bound peptide is inherently unstable and requires the ...
... calgranulin-B explanation free. What is calgranulin-B? Meaning of calgranulin-B medical term. What does calgranulin-B mean? ... Looking for online definition of calgranulin-B in the Medical Dictionary? ... Calgranulin-B , definition of calgranulin-B by Medical dictionary https://medical-dictionary.thefreedictionary.com/calgranulin- ... redirected from calgranulin-B) S100A9. A gene on chromosome 1q21 that encodes a member of the S100 family of proteins, which ...
RATIONALE: Measuring levels of calgranulin A and calgranulin B in the blood of patients with breast cancer in the laboratory ... PURPOSE: This laboratory study is measuring calgranulin A and calgranulin B levels in the blood of patients with newly ... Blood draw following diagnosis of breast cancer to assess levels of circulating tumor markers, calgranulin A and calgranulin B. ... Correlation between circulating levels of calgranulin A and calgranulin B and the presence of estrogen receptor negative breast ...
The calgranulin A/B heterodimer is known as LEUKOCYTE L1 ANTIGEN COMPLEX. Calgranulin B is expressed at high concentrations in ... or heterocomplexes with CALGRANULIN A and a variety of other proteins. ... GRANULOCYTES during early monocyte differentiation, and serum calgranulin B levels are elevated in many inflammatory disorders ... Cellular Distribution and Gene Expression Pattern of Metastasin (S100A4), Calgranulin A (S100A8), and Calgranulin B (S100A9) in ...
Background of S100A9 / Calgranulin-B / MRP14 antibody. The calcium-binding, migration inhibitory factor-related proteins, MRP-8 ... Human tonsil Frozen section stained with Biotin conjugated S100A9 / Calgranulin-B / MRP14 Antibody Cat.-No BM4026B. *BM4026B ... Alternative names for S100A9 / Calgranulin-B / MRP14 antibody. S100-A9, CAGB, MRP-14, S100 calcium-binding protein A9, ...
S100/Calgranulin-Mediated Inflammation Accelerates Left Ventricular Hypertrophy and Aortic Valve Sclerosis in Chronic Kidney ... S100/Calgranulin-Mediated Inflammation Accelerates Left Ventricular Hypertrophy and Aortic Valve Sclerosis in Chronic Kidney ... S100/Calgranulin-Mediated Inflammation Accelerates Left Ventricular Hypertrophy and Aortic Valve Sclerosis in Chronic Kidney ... S100/Calgranulin-Mediated Inflammation Accelerates Left Ventricular Hypertrophy and Aortic Valve Sclerosis in Chronic Kidney ...
Additionally, in response to infection, the neutrophil protein calgranulin C (S100A12) was found to be increased in the feces ... Additionally, in response to infection, the neutrophil protein calgranulin C (S100A12) was found to be increased in the feces ... Shank, Janette Marie, "The Roles of Calprotectin and Calgranulin C in Campylobacter jejuni Infection. " Masters Thesis, ...
S100A12 (EN-RAGE, calgranulin) is a member of the S100 protein family, which, in humans, consists of twenty five EF-hand (alpha ... You are here: Home Products by Molecule of Interest S100A12 (S100 calcium-binding protein A12, Calgranulin-C, CAGC, CGRP, ... References to S100A12 (S100 calcium-binding protein A12, Calgranulin-C, CAGC, CGRP, Neutrophil S100 protein, Calcium-binding ... S100A12 (S100 calcium-binding protein A12, Calgranulin-C, CAGC, CGRP, Neutrophil S100 protein, Calcium-binding protein in ...
ELISA Calgranulin-B,Migration inhibitory factor-related protein 14,MRP14,MRP-14,Oryctolagus cuniculus,p14,Protein S100-A9, ... ELISA Calgranulin-B,Migration inhibitory factor-related protein 14,MRP14,MRP-14,Oryctolagus cuniculus,p14,Protein S100-A9, ... Product name : ELISA Calgranulin-B,Migration inhibitory factor-related protein 14,MRP14,MRP-14,Oryctolagus cuniculus,p14, ... E1793Rb ELISA Calgranulin-B,Migration inhibitory factor-related protein 14,MRP14,MRP-14,Oryctolagus cuniculus,p14,Protein S100- ...
Calgranulin B, Mab anti_Human; Clone CF_557, Biotin conj. \ ACL2712B for more molecular products just contact us ... Index / Accu / Calgranulin B, Mab anti_Human; Clone CF_557, Biotin conj. / Product Detail : ACL2712B Calgranulin B, Mab anti_ ... We have also other products like : Calgranulin B, Mab anti_Human; Clone CF_557, Biotin conj.. Related products : Calgranulin B ... Calgranulin B, Mab anti_Human; Clone CF_557, Biotin conj. Human samples 80 % of the research is conducted on human samples. ...
Tandem mass spectrometry analysis identified them asmyeloperoxidase chain A (MPO-A), alpha-defensin, and calgranulin. ELISA, ... Tandem mass spectrometry analysis identified them asmyeloperoxidase chain A (MPO-A), alpha-defensin, and calgranulin. ELISA, ... Identification of myeloperoxidase, alpha-defensin and calgranulin in calcium oxalate renal stones. ... alpha-defensin and calgranulin in calcium oxalate renal stones.. Clinica Chemica Acta: International Journal of Clinical ...
Calgranulin Brophy MB, Nolan EM (March 2015). "Manganese and microbial pathogenesis: sequestration by the Mammalian immune ...
Calgranulin-A; CAGA; CGLA; MA387; NIF. The encoded protein has an amino acid length of 93 and a mass of 10.8 kDa. CP-10 is a ...
S100 Calcium Binding Protein A8/Calgranulin A) ELISA Kit OSCAR DIAGNOSTIC SERVICES PVT. LTD.is an India based Company in Delhi. ... Chicken S100A8 (S100 Calcium Binding Protein A8/Calgranulin A) ELISA Kit » Chicken S100A8 (S100 Calcium Binding Protein A8/ ... Calgranulin A) ELISA Kit. Chicken S100A8 (S100 Calcium Binding Protein A8/Calgranulin A) ELISA Kit. Chicken S100A8 (S100 ... Chicken S100A8 (S100 Calcium Binding Protein A8/Calgranulin A) ELISA Kit. Chicken S100A8 (S100 Calcium Binding Protein A8/ ...
Mouse S100A8/Calgranulin A PicoKine ELISA Kit *Detection Target: Protein S100-A8 ...
Calgranulin AB, alpha-Synuelein (P37377), beta-Synuclein (Q63754), HNP 22; Neural nuclear proteins: NeuN-1, S/G(2) nuclear ... Calgranulin AB, alpha-Synuclein (P37377), beta-Synuclein (Q63754), HNP 22; Neural nuclear proteins: NeuN-1, S/G(2) nuclear ... Calgranulin AB, alpha-Synuclein (P37377), beta-Synuclein (Q63754), HNP 22; Neural nuclear proteins: NeuN-1, S/G(2) nuclear ... Calgranulin AB, alpha-Synuclein (P37377), beta-Synuclein (Q63754), HNP 22; Neural nuclear proteins: NeuN-1, S/G(2) nuclear ...
4. The method of any one of claims 1 to 3, wherein said method comprises the detection of M2BP, calgranulin B, CD59, profilin, ... In another particular embodiment, the methods of the invention comprise the detection of M2BP, calgranulin B, CD59, profilin, ... The discovered candidate biomarkers include calgranulin A, serum amyloid A-4 protein and related isoforms, haptoglobin-related ... Further validation of calgranulin A by ELISA (n=20 for each group, p=0.039) and tetranectin by immunoblotting (n=35 for each ...
Serum calgranulin C is a highly sensitive autoinflammation activity indicator in patients with familial periodic fevers].. [ ... To determine the possibility of using the serum proinflammatory calcium-binding protein, or calgranulin C (S100A12), to assess ...
Calgranulin B / metabolism * Cell Proliferation * Chronic Disease * Female * Inflammation / enzymology * Inflammation / ...
S100A8 (calgranulin A, MRP8) and S100A9 (calgranulin B, MRP14) are members of the calcium-binding protein family S100 genes (54 ... 33), the proto-oncogene Bcl-2, and two calcium-binding protein genes, S100A8 (calgranulin A, MRP8) and S100A9 (calgranulin B, ... calgranulin A, MRP8) and S100A9 (calgranulin B, MRP14), apoptosis inhibitor BIRC5 (surviving, baculoviral IAP repeat-containing ... Similarly, calgranulin expression extended from the intermediate layers to superficial cells as the CIN grade progressed (P , ...
S100 calcium binding protein A9 calgranulin B. *S100 calcium-binding protein A9 ...
... some researchers have found that the molecule calgranulin is able to inhibit calcium oxalate crystal growth.[41] Calgranulin is ... Given the large amounts of calcium oxalate in the urine, and considering its potency, calgranulin could become an important ...
S100A8/12, calgranulin A/B heterodimer ligand for RAGE.. Molecular and Cellular Mechanisms of Cardiovascular Disorders in ...
S100 calcium binding protein A9,calgranulin B,epidermal differentiation complex expressed in monocytes and granulocytes *S100A9 ...
S100 calcium-binding protein A12 (calgranulin C) antibody. *S100 calcium-binding protein A12 antibody ...
Other names for calprotectin include MRP8-MRP14, calgranulin A and B, cystic fibrosis antigen, L1, 60BB antigen, and 27E10 ...
Interleukin 22 inhibits intracellular growth of Mycobacterium tuberculosis by enhancing calgranulin A expression. J. Infect. ...
Calgranulin A * Chromosome Mapping * Chromosomes, Human, Pair 7 / ultrastructure* * Cystic Fibrosis / genetics* ...
Bcl2 protein family; S100/calgranulin; Cancer regression; Drp1; Receptor for advanced glycated endproducts (RAGE); ...
Compound: calgranulin c. Species: Homo sapiens [TaxId:9606]. Database cross-references and differences (RAF-indexed): *Uniprot ... Compound: calgranulin c. Species: Homo sapiens [TaxId:9606]. Database cross-references and differences (RAF-indexed): *Uniprot ... Compound: calgranulin c. Species: Homo sapiens [TaxId:9606]. Database cross-references and differences (RAF-indexed): *Uniprot ... Compound: calgranulin c. Species: Homo sapiens [TaxId:9606]. Database cross-references and differences (RAF-indexed): *Uniprot ...
  • Calgranulin B (S100A9/MRP14): A Key Molecule in Idiopathic Pulmonary Fibrosis? (semanticscholar.org)
  • Human tonsil Frozen section stained with Biotin conjugated S100A9 / Calgranulin-B / MRP14 Antibody Cat. (acris-antibodies.com)
  • Approach and Results- A bacterial artificial chromosome of the human S100/calgranulin gene cluster containing the genes and regulatory elements for S100A8, S100A9, and S100A12 was expressed in C57BL/6J mouse (hBAC-S100) to generate a novel humanized mouse model. (ahajournals.org)
  • ELISA Calgranulin-B,Migration inhibitory factor-related protein 14,MRP14,MRP-14,Oryctolagus cuniculus,p14,Protein S100-A9,Rabbit,S100 calcium-binding protein A9,S100A9 rabbit polyclonal These antibodies are very stable and can be stored up to 2 months at fridge temperature under 10C. (antibody-antibodies.com)
  • These transgenic mice express S100a9 (S100 calcium binding protein A9 (calgranulin B)), a myeloid-related protein, and GFP under the control of the hematopoietic cell-specific H2-K promoter. (jax.org)
  • Human MRP14, Granulocytes, stimulated Monocytes and Macrophages: This clone identifies the Ca2+-binding 14 kDa subunit of the inflammatory L-1 protein complex, also called S100A9 or Calgranulin B. It is useful for the characterization of circulating granulocytes or inflammatory infiltrates of the myelo-monocytic lineage which express MRP14 differently depending on the inflammatory status of the disease. (antibodies-online.com)
  • MRP8 (Calgranulin A, 10.8 kDa) and MRP14 (Calgranulin B, 13.2 kDa) belong to the S-100 protein family (S100A8 and S100A9) and can form Ca2+ dependent homo- or hetero-complexes of various composition. (acris-antibodies.com)
  • Mrp exists as a heterodimer of Mrp-14 (S100A9 or calgranulin B) and Mrp-8 (S100A8 or calgranulin A), with prior studies demonstrating an important role for the Mrp complex in the inflammatory response to injury. (ahajournals.org)
  • This antibody identifies the Ca2+-binding light subunit of the inflammatory L-1 protein complex, also called S100A8 or Calgranulin A. It is useful for the identification of the 8 kDa subunit in various immunological techniques. (antikoerper-online.de)
  • MRP8 (10.8 kDa) is also known as p8, L1 light chain, calgranulin A, and cystic fibrosis antigen (CFA). (biomedcentral.com)
  • Additionally, in response to infection, the neutrophil protein calgranulin C (S100A12) was found to be increased in the feces of both ferrets and humans infected with C. jejuni , while calprotectin (another neutrophil protein) was not. (tennessee.edu)
  • S100A12 (EN-RAGE, calgranulin) is a member of the S100 protein family, which, in humans, consists of twenty five EF-hand (alpha helix-loop-alpha helix), calcium-binding proteins, of which the vast majority is in homodimer, heterodimer or more complex forms. (biovendor.com)
  • 1999) reported that RAGE is a central cell surface receptor for S100A12, which they referred to as EN-RAGE (Extracellular Newly identified RAGE-binding protein), and related members of the S100/calgranulin superfamily. (biovendor.com)
  • Interaction of EN-RAGE (S100A12) with cellular RAGE on endothelium, mononuclear phagocytes, and lymphocytes triggered cellular activation, with generation of key proinflammatory mediators. (biovendor.com)
  • S100A12, also known as extracellular newly identified RAGE or calgranulin C, is a proinflammatory protein predominantly secreted by neutrophils 12 , 13 . (jrheum.org)
  • This includes a member of the S100/calgranulin family termed S100A12, calgranulin C, or extracellular newly identified RAGE-binding proteins (EN-RAGE). (ahajournals.org)
  • Excessive amounts of calgranulin A/B in extracellular compartments of tissues correlates with a variety of inflammatory disorders. (intechopen.com)
  • Expression and release of calgranulin A/B in the extracellular milieu can be triggered by IL-1α. (intechopen.com)
  • Extracellular calgranulin A/B can then bind to and activate toll like receptor 4 (TLR 4), which in turn initiates further expression of pro-inflammatory mediators such as IL-1, IL-6, and IL-8. (intechopen.com)
  • The term calprotectin specifically refers to the Calgranulin A and B heterodimer that is formed by a non-covalent interaction. (intechopen.com)
  • Patients undergo a blood draw following diagnosis of breast cancer to assess levels of circulating tumor markers, calgranulin A and calgranulin B. Serum samples are evaluated by enzyme-linked immunosorbent assay for tumor marker expression. (clinicaltrials.gov)
  • Serum calgranulin C is a highly sensitive autoinflammation activity indicator in patients with familial periodic fevers]. (cdc.gov)
  • For example, granulocytes depend on the intracellular calgranulin A/B complex to adequately participate in cell adhesion, cytokinesis, cytokine secretion, and activation of the respiratory burst. (intechopen.com)
  • Calgranulin A expression may also be important in promoting the induction of a regulatory macrophage phenotype that down-regulates inflammation and facilitates a healing response. (intechopen.com)
  • RATIONALE: Measuring levels of calgranulin A and calgranulin B in the blood of patients with breast cancer in the laboratory may help doctors identify and learn more about biomarkers related to breast cancer. (clinicaltrials.gov)
  • 3. The method of any one of claim 1 or 2, wherein at least one of said oral cancer biomarkers is selected from the group consisting of M2BP, calgranulin B, CD59, profilin, and catalase. (freepatentsonline.com)
  • In the urinary tract, over expression of calgranulin A is not specific for infection. (intechopen.com)
  • Determine circulating levels of calgranulin A and calgranulin B in patients with estrogen receptor negative or estrogen receptor positive, newly diagnosed, primary stage I-III adenocarcinoma of the breast. (clinicaltrials.gov)
  • Conclusions- Myeloid-derived human S100/calgranulin is associated with the development of cardiac hypertrophy and ectopic cardiac calcification in a receptor for advanced glycation end products-dependent manner in a mouse model of CKD. (ahajournals.org)
  • In murine models, blockade of EN-RAGE/RAGE quenched delayed-type hypersensitivity and inflammatory colitis by arresting activation of central signaling pathways and expression of inflammatory gene mediators. (biovendor.com)
  • The interaction of these cytokines and chemokines with calgranulin A can create an autocrine / paracine mediated pro-inflammatory feedback loop that does not necessarily resolve infection. (intechopen.com)
  • Tandem mass spectrometry analysis identified them asmyeloperoxidase chain A (MPO-A), alpha-defensin, and calgranulin. (aku.edu)
  • We tested the hypothesis that human S100/calgranulin would accelerate cardiovascular disease in mice subjected to CKD. (ahajournals.org)
  • Shank, Janette Marie, "The Roles of Calprotectin and Calgranulin C in Campylobacter jejuni Infection. (tennessee.edu)
  • Identification of myeloperoxidase, alpha-defensin and calgranulin in c" by Shamim Mushtaq, Anwar Ali Siddiqui et al. (aku.edu)
  • Identification of myeloperoxidase, alpha-defensin and calgranulin in calcium oxalate renal stones. (aku.edu)